Claims
- 1. A purified polynucleotide consisting of a nucleic acid sequence encoding the polypeptide having the sequence shown in SEQ ID NO:2, or its complement.
- 2. An isolated polynucleotide fragment comprising a nucleic acid sequence selected from the group consisting of:(a) a nucleic acid sequence encoding the polypeptide set forth as amino acid residues 1 to 224 of SEQ ID NO:2; (b) a nucleic acid sequence encoding the polypeptide set forth as amino acid residues 2 to 224 of SEQ ID NO:2; (c) a nucleic acid sequence encoding the polypeptide set forth as amino acid residues 30 to 224 of SEQ ID NO:2; (d) a nucleic acid sequence encoding the polypeptide set forth as amino acid residues 30 to 51 of SEQ ID NO:2; (e) a nucleic acid sequence encoding a fragment of the polypeptide set forth as amino acid residues 1 to 244 of SEQ ID NO:2, said fragment having protease inhibiting activity; (f) a nucleic acid sequence encoding at least 30 contiguous amino acid residues of SEQ ID NO:2 wherein said polynucleotide fragment encodes a polypeptide capable of inducing an antibody specific to the polypeptide of SEQ ID NO:2; (g) the nucleic acid sequence of a polynucleotide which specifically hybridizes to a polynucleotide consisting of the complement of SEQ ID NO:1, wherein said hybridizes includes incubation in Church buffer with 100 μg/ml denatured herring sperm DNA followed by washing sequentially with 2×SSC/0.1% SDS and 0.2×SSC/0.1% SDS at 65° C.; and (h) a nucleic acid sequence complementary to the nucleic acid sequence of (a), (b), (c), (d), (e), (f) or (g).
- 3. The isolated polynucleotide of claim 2 wherein the nucleic acid sequence is (a).
- 4. The isolated polynucleotide of claim 2 wherein the nucleic acid sequence is (b).
- 5. The isolated polynucleotide of claim 2 wherein the nucleic acid sequence is (c).
- 6. The isolated polynucleotide of claim 2 wherein the nucleic acid sequence is (d).
- 7. The isolated polynucleotide of claim 2 wherein the nucleic acid sequence is (e).
- 8. The isolated polynucleotide of claim 2 wherein the nucleic acid sequence is (f).
- 9. The isolated polynucleotide of claim 8 wherein the nucleic acid sequence encodes at least 50 contiguous amino acid residues of SEQ ID NO:2.
- 10. The isolated polynucleotide of claim 2 wherein the nucleic acid sequence is (g).
- 11. The isolated polynucleotide of claim 10 wherein the polynucleotide encodes a polypeptide having protease inhibiting activity.
- 12. The isolated polynucleotide of claim 2 wherein said polynucleotide is DNA and further wherein said nucleic acid sequence is (a), (b), (c), (d), (e) or (f).
- 13. A recombinant vector comprising the DNA of claim 12.
- 14. A recombinant host cell comprising the DNA of claim 12.
- 15. A polynucleotide comprising the DNA of claim 12 linked to a heterologous regulatory sequence which controls gene expression.
- 16. A process for producing a polypeptide comprising expressing from the host cell of claim 14 the encoded polypeptide and recovering said polypeptide.
- 17. The isolated polynucleotide of claim 5 comprising the nucleic acid sequence shown as nucleotides 88 to 672 in SEQ ID NO:1.
- 18. The isolated polynucleotide of claim 2 consisting of a nucleic acid sequence selected from the group consisting of:(a) a nucleic acid sequence encoding the polypeptide set forth as amino acid residues 1 to 224 of SEQ ID NO:2; (b) a nucleic acid sequence encoding the polypeptide set forth as amino acid residues 2 to 224 of SEQ ID NO:2; (c) a nucleic acid sequence encoding the polypeptide set forth as amino acid residues 30 to 224 of SEQ ID NO:2; (d) a nucleic acid sequence encoding the polypeptide set forth as amino acid residues 30 to 51 of SEQ ID NO:2; (e) a nucleic acid sequence encoding a fragment of the polypeptide set forth as amino acid residues 1 to 244 of SEQ ID NO:2, said fragment having protease inhibiting activity; (f) a nucleic acid sequence encoding at least 30 contiguous amino acid residues of SEQ ID NO:2; and (g) a nucleic acid sequence complementary to the nucleic acid sequence of (a), (b), (c), (d), (e) or (f).
- 19. An isolated polynucleotide comprising the polynucleotide fragment of claim 18 fused to a heterologous polynucleotide.
- 20. An isolated polynucleotide fragment comprising a nucleic acid sequence selected from the group consisting of:(a) a nucleic acid sequence encoding the polypeptide encoded by the human cDNA contained in ATCC Deposit No. 75946; (b) a nucleic acid sequence encoding the mature polypeptide encoded by the human cDNA contained in ATCC Deposit No. 75946; (c) a nucleic acid sequence encoding the amino acid sequence of a fragment of the polypeptide encoded by the human cDNA contained in ATCC Deposit No. 75946, said fragment having protease inhibiting activity; (d) a nucleic acid sequence encoding at least 30 contiguous amino acids of the polypeptide encoded by the human cDNA contained in ATCC Deposit No. 75946 wherein said polynucleotide fragment encodes a polypeptide capable of inducing an antibody specific to the polypeptide encoded by the human cDNA contained in ATCC Deposit No. 75946; (e) the nucleic acid sequence of a polynucleotide which specifically hybridizes to the complement of the coding portion of the human cDNA contained in ATCC Deposit No. 75946, wherein said hybridizes includes incubation in Church buffer with 100 μg/ml denatured herring sperm DNA followed by washing sequentially with 2×SSC/0.1% SDS and 0.2×SSC/0.1% SDS at 65° C.; and (f) a nucleic acid sequence complementary to the nucleic acid sequence of (a), (b), (c), (d) or (e).
- 21. The isolated polynucleotide of claim 20 wherein said nucleic acid sequence is (a).
- 22. The isolated polynucleotide of claim 20 wherein said nucleic acid sequence is (b).
- 23. The isolated polynucleotide of claim 20 wherein said nucleic acid sequence is (c).
- 24. The isolated polynucleotide of claim 20 wherein said nucleic acid sequence is (d).
- 25. The isolated polynucleotide of claim 24 wherein said nucleic acid sequence encodes at least 50 contiguous amino acids of the polypeptide encoded by the human cDNA contained in ATCC Deposit No. 75946.
- 26. The isolated polynucleotide of claim 20 wherein said nucleic acid sequence is (e).
- 27. The isolated polynucleotide of claim 26 wherein said polynucleotide encodes a polypeptide having protease inhibiting activity.
- 28. The isolated polynucleotide of claim 20 wherein said polynucleotide is DNA and further wherein said nucleic acid sequence is (a), (b), (c) or (d).
- 29. A recombinant vector comprising the DNA of claim 28.
- 30. A recombinant host cell comprising the DNA of claim 28.
- 31. The isolated DNA of claim 28 linked to regulatory sequence which controls gene expression.
- 32. A process for producing a polypeptide comprising expressing from the host cell of claim 30 the encoded polypeptide and recovering said polypeptide.
- 33. The isolated polynucleotide of claim 20 consisting of a nucleic acid sequence selected from the group consisting of:(a) a nucleic acid sequence encoding the polypeptide encoded by the human cDNA contained in ATCC Deposit No. 75946; (b) a nucleic acid sequence encoding the mature polypeptide encoded by the human cDNA contained in ATCC Deposit No. 75946; (c) a nucleic acid sequence encoding the amino acid sequence of a fragment of the polypeptide encoded by the human cDNA contained in ATCC Deposit No. 75946, said fragment having protease inhibiting activity; (d) a nucleic acid sequence encoding at least 30 contiguous amino acids of the polypeptide encoded by the human cDNA contained in ATCC Deposit No. 75946; (e) a nucleic acid sequence complementary to the nucleic acid sequence of (a), (b), (c) or (d).
- 34. An isolated polynucleotide comprising the polynucleotide fragment of claim 33 fused to a heterologous polynucleotide.
Parent Case Info
This application is a continuation-in-part of, and claims priority under 35 U.S.C. §120 to, co-pending U.S. patent application Ser. No. PCT US94/14498 filed Dec. 13, 1994.
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Number |
Name |
Date |
Kind |
5643752 |
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Jul 1997 |
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Oct 1990 |
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Non-Patent Literature Citations (3)
Entry |
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Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
PCT/US94/14498 |
Dec 1994 |
US |
Child |
08/463261 |
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US |