Claims
- 1. A method of identifying an antifungal compound comprising the steps of:
- (a) contacting fungal cells with a test compound and with a membrane potential indicator dye, and
- (b) detecting an increasing accumulation of said dye and retention of dye despite reduction of fungal cell viability.
- 2. A method of identifying an antibacterial compound comprising the steps of:
- (a) contacting bacterial cells with a test compound and with a membrane potential indicator dye, and
- (b) detecting an increasing accumulation of said dye and retention of dye despite reduction of bacterial cell viability.
- 3. A method of identifying an antimicrobial compound comprising the steps of:
- (a) contacting microbial cells with a test compound and with a membrane potential indicator dye, and
- (b) detecting an increasing accumulation of said dye and retention of dye despite reduction of microbial cell viability.
- 4. The method of any one of claims 1 through 3 further comprising the step of determining microbial cell viability.
- 5. The method of any one of claims 1 through 3 further comprising the steps of:
- (a) contacting a mammalian cell with said test compound and with said membrane potential indicator dye, and
- (b) detecting no substantial change in dye uptake.
- 6. The method of any one of claims 1 through 3 further comprising the step of assaying said test compound for the ability to inhibit growth of microbial cells or to kill microbial cells.
- 7. The method of any one of claims 1 through 3 further comprising the steps of assaying said test compound for in vivo oral availability or oral activity.
- 8. The method of any one of claims 1 through 3 wherein said membrane potential indicator dye is DiOC.sub.6 (3) (3,3'-dihexyloxacarbocyanine iodide).
- 9. The method of any one of claims 1 through 3 wherein said membrane potential indicator dye is JC-1 (5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine halide, or CBIC.sub.2 (3)).
- 10. The method of any one of claims 1 through 3 wherein said membrane potential indicator dye is dihydrorhodamine 123.
- 11. The method of any one of claims 1 through 3 wherein said membrane potential indicator dye is MitoTracker.RTM. Red CM-H.sub.2 Xros (M-7513; C.sub.32 H.sub.33 ClN.sub.2 O).
Parent Case Info
This application claims priority based on U.S. Provisional Application Serial No. 60/143,485 filed Jul. 12, 1999, U.S. Provisional Application Serial No. 60/109,905 filed Nov. 25, 1998, and U.S. Provisional Application Serial No. 60/101,778 filed Sep. 25, 1998.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
4859584 |
Horan et al. |
Aug 1989 |
|
Non-Patent Literature Citations (3)
Entry |
Wang et al., "Susceptibility of Melanized and Nonmelanized Crypotococcus neoformans to the Melanin-Binding Compounds Trifluoperazine and Chloroquine"Antimicrobial Agents and Chemotherapy 40:541-545 (1996). |
Abrahamson et al., "Mechanism of Action of XMP Antifungal Peptides: Factors that Influence Activity and Subcellular Localization", Abstract for ICAAC meeting, Sep. 28-Oct. 1, 1997, Toronto, Canada. |
Little et al., "XMP Antifungal Peptides Demonstrate Enhanced Selectivity and Oral Availability" Abstract for ICAAC meeting, Sep. 24-27, 1998, San Diego, California. |