Claims
- 1. An isolated polynucleotide comprising SEQ ID NO: 1 wherein the nucleic acid at 1792 is A or C.
- 2. An isolated polynucleotide comprising a nucleic acid that encodes a polypeptide comprising SEQ ID NO: 2, wherein the amino acid 598 is Thr or Pro
- 3. An isolated polynucleotide comprising SEQ ID NO: 3.
- 4. An isolated polynucleotide comprising a nucleic acid that encodes a polypeptide comprising SEQ ID NO: 4.
- 5. An isolated polynucleotide comprising a molecule selected from the group consisting of:
a) A polynucleotide that encodes a polypeptide comprising amino acid residues 384-687 of SEQ ID NO: 2, wherein the amino acid at 598 is Thr or Pro; b) A polynucleotide that encodes a polypeptide comprising amino acid residues 379-687 of SEQ ID NO: 2, wherein the amino acid at 598 is Thr or Pro; c) A polynucleotide that encodes a polypeptide comprising amino acid residues 389-685 of SEQ ID NO: 4; d) A polynucleotide that encodes a polypeptide comprising amino acid residues 379-685 of SEQ ID NO: 4; e) A polynucleotide that encodes a polypeptide comprising amino acid residues 449-687 of SEQ ID NO: 2, wherein the amino acid at 598 is Pro or Thr; f) A polynucleotide that encodes a polypeptide comprising amino acid residues 449-685 of SEQ ID NO: 4 g) A polynucleotide that encodes a fragment of a polypeptide described in (a-g), wherein the fragment interacts with a signal transduction factor; h) An isolated nucleic acid molecule that hybridizes to either strand of a denatured, double-stranded DNA comprising the polynucleotide of any one of a)-g) under conditions of moderate stringency in 50% formamide and 6×SSC, at 42° C. with washing conditions of 60° C., 0.5×SSC, 0.1% SDS; i) An isolated nucleic acid molecule that encodes a polypeptide that is at least 85% identical to the polypeptides described in a)-g), wherein the polypeptide interacts with a signal transduction factor; j) A polynucleotide that is degenerate to any of the polynucleotides of a)-i).
- 6. An expression vector comprising a polynucleotide of claim 5.
- 7. An expression vector comprising a polynucleotide that encodes a polypeptide comprising SEQ ID NO: 2, wherein the amino acid residue at 598 is Pro or Thr.
- 8. An expression vector comprising a polynucleotide that encodes a polypeptide comprising SEQ ID NO: 4.
- 9. A host cell comprising the vector of claim 5.
- 10. A process of preparing a polypeptide, the process comprising culturing a host cell of claim 9 under conditions promoting expression of the polypeptide.
- 11. A process of preparing a polypeptide, the process comprising culturing a host cell transformed with a vector of claim 7 under conditions promoting expression of the polypeptide.
- 12. A polypeptide selected from the group consisting of:
a) A polypeptide comprising SEQ ID NO: 2, wherein the amino acid at 598 is Thr or Pro; b) A polypeptide comprising SEQ ID NO: 4. c) A polypeptide comprising amino acids 449-685 of SEQ ID NO: 4; d) A polypeptide comprising amino acids 449-687 of SEQ ID NO: 2, wherein the amino acid at 598 is Thr or Pro; e) A polypeptide comprising amino acids 384-687 of SEQ ID NO: 2, wherein the amino acid at 598 is Thr or Pro; f) A polypeptide comprising amino acids 379-687 of SEQ ID NO: 2, wherein the amino acid at 598 is Thr or Pro g) A polypeptide comprising amino acids 379-685 of SEQ ID NO: 4; h) A polypeptide comprising amino acids 389-685 of SEQ ID NO: 4; i) A polypeptide comprising a fragment of a polypeptide of a)-h) wherein the fragment interacts with a signal transduction factor. j) A polypeptide that is at least 85% identical to a polypeptide of a)-g), wherein the polypeptide interacts with a signal transduction factor.
- 13. An antibody that is specific to a polypeptide of claim 10.
- 14. A method for screening for an agonist or antagonist of IL-1 comprising:
Contacting a polypeptide of claim 8 with an IL-1 family member and an IL-1 receptor family member in the presence of a candidate compound, and comparing the interaction of the polypeptide in the presence of the candidate compound with the interaction in the absence of the compound, whereby a compound the modulates the interaction of the polypeptide is identified as an agonist or antagonist of the polypeptide of claim 8.
Parent Case Info
[0001] This application claims the benefit under U.S.C. 119(e) of U.S. provisional application serial No. 60/244,831, filed Oct. 31, 2000. All of which is incorporated by reference herein.
Provisional Applications (1)
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Number |
Date |
Country |
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60244831 |
Oct 2000 |
US |