Claims
- 1. A method of preparing an immobilized enzyme composition comprising:
- a. providing an aqueous-fluid-absorbing composition comprising water-insoluble alkali salts of aqueous alkali saponified gelatinized-starch-polyacrylonitrile graft polymers, which alkali salts were prepared by saponifying the product resulting from graft polymerization of acrylonitrile onto gelatinized starch, and which alkali salts contain gelatinized starch (GS) and saponified polyacrylonitrile (HPAN) in molar ratios of from about 1:1.5 to 1.9 GS:HPAN, said water-insoluble alkali salts of aqueous alkali saponified gelatinized-starch-polyacrylonitrile graft polymers being water-insoluble solids capable of absorbing in excess of 300 parts of water per part of said water-insoluble solids;
- b. mixing a solution of enzyme with the aqueous-fluid-absorbing composition whereby the solution of enzyme is absorbed into the fluid-absorbing composition thereby swelling said composition; and
- c. adding to said swollen composition an amount of water-soluble mineral salts sufficient to shrink the composition to an extent sufficient to entrap the enzyme within its matrices.
- 2. A method of preparing an immobilized enzyme composition as described in claim 1 wherein the aqueous-fluid-absorbing composition provided in step (a) is in the form of a film.
- 3. A method of preparing an immobilized enzyme composition as described in claim 1 wherein the enzyme is .alpha.-amylase or glucoamylase.
- 4. A method of preparing an immobilized enzyme composition as described in claim 1 wherein the aqueous-fluid-absorbing composition is in the form of a film, wherein the enzyme is glucoamylase, and wherein 0.25 g. (dry basis) of the film absorbs 10 ml. of an aqueous solution of the glucoamylase having about 160 glucoamylase units, and the resulting swollen film is shrunk with 10 ml. of a 1% (by weight) calcium chloride solution.
- 5. An immobilized enzyme composition produced by the process of claim 1.
- 6. A method of preparing an immobilized enzyme composition comprising:
- a. providing an aqueous-fluid-absorbing composition comprising water-insoluble alkali salts of aqueous alkali saponified gelatinized-starch-polyacrylonitrile graft polymers, which alkali salts were prepared by saponifying the product resulting from graft polymerization of acrylonitrile onto gelatinized starch, and which alkali salts contain gelatinized starch (GS) and saponified polyacrylonitrile (HPAN) in molar ratios of from about 1:1.5 to 1.9 GS:HPAN, said water-insoluble alkali salts of aqueous alkali saponified gelatinized-starch-polyacrylonitrile graft polymers being water-insoluble solids capable of absorbing in excess of 300 parts of water per part of said water-insoluble solids; and
- b. mixing a solution of the enzyme and a coupling reagent with the fluid-absorbing composition thereby covalently bonding said enzyme to said composition and simultaneously swelling said composition.
- 7. A method of preparing an immobilized enzyme composition as described in claim 6 and including the following step:
- c. adding to said swollen composition an amount of water-soluble mineral salts sufficient to shrink said composition.
- 8. A method of preparing an immobilized enzyme composition as described in claim 6 wherein the aqueous-fluid-absorbing composition provided in step (a) is in the form of a film.
- 9. A method of preparing an immobilized enzyme composition as described in claim 6 wherein the enzyme is .alpha.-amylase or glucoamylase.
- 10. A method of preparing an immobilized enzyme composition as described in claim 6 wherein the aqueous-fluid-absorbing composition is in the form of a film, wherein the enzyme is .alpha.-amylase, and wherein 0.25 g. (dry basis) of the film absorbs 10 ml. of an aqueous solution of the .alpha.-amylase, and the resulting swollen film is shrunk with 10 ml. of a 1% (by weight) calcium chloride solution.
- 11. An immobilized enzyme composition produced by the process of claim 6.
Parent Case Info
This is a division of application Ser. No. 456,911, filed Apr. 3, 1974, now U.S. Pat. No. 3.935,099
US Referenced Citations (3)
Number |
Name |
Date |
Kind |
3425971 |
Gugliemelli et al. |
Feb 1969 |
|
3661815 |
Smith |
May 1972 |
|
3745088 |
Mehltretter et al. |
Jul 1973 |
|
Non-Patent Literature Citations (1)
Entry |
zaborsky, O., Immobilized Enzymes, CRC Press, Cleveland, Ohio, 1973, (pp. 83-88). |
Divisions (1)
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Number |
Date |
Country |
Parent |
456911 |
Apr 1974 |
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