Claims
- 1. An isolated DNA molecule encoding at least two different inward rectifier, G-protein activated, mammalian, potassium Kir3.0 polypeptides, wherein said nucleic acid is characterized by its ability to cause a change in potassium flow across a Xenopus oocyte cell membrane upon expression therein.
- 2. A nucleic acid acccording to claim 1, wherein said mammalian Kir3.0 polypeptides are Kir3.1/KGA and Kir3.2.
- 3. A nucleic acid acccording to claim 1, wherein said mammalian Kir3.0 polypeptides are Kir3.1/KGA and Kir3.3.
- 4. A nucleic acid acccording to claim 1, wherein said mammalian Kir3.0 polypeptides are Kir3.2 and Kir3.3.
- 5. A nucleic acid according to claim 2, wherein said Kir3.1/KGA polypeptide has the amino acid sequence of SEQ ID NO:2.
- 6. A method of producing a functional Kir3.0 channel in an expression host cell, the method comprising:
introducing into said expression host cell a nucleic acid encoding a first mammalian Kir3.0 polypeptide and a nucleic acid encoding a second mammalian Kir3.0 polypeptide into said heterologous cell under conditions that permit expression of said nucleic acids; wherein said mammalian Kir3.0 polypeptides assemble to form a functional Kir3.0 in said expression host cell.
- 7. A method according to claim 6, wherein said nucleic acid encoding said first mammalian Kir3.0 polypeptide and said nucleic acid encoding said second mammalian Kir3.0 polypeptide are present on a single vector.
- 8. A method according to claim 6, wherein said nucleic acid encoding said first mammalian Kir3.0 polypeptide and said nucleic acid encoding said second mammalian Kir3.0 polypeptide are present on different vectors.
- 9. A method acccording to claim 6, wherein said mammalian Kir3.0 polypeptides are Kir3.1/KGA and Kir3.2.
- 10. A method acccording to claim 6, wherein said mammalian Kir3.0 polypeptides are Kir3.1 /KGA and Kir3.3.
- 11. A method acccording to claim 6, wherein said mammalian Kir3.0 polypeptides are Kir3.2 and Kir3.3.
- 12. A host cell comprising a functional heterologous Kir3.0 channel;
wherein said functional Kir3.0 channel comprises a first Kir3.0 polypeptide and a second mammalian Kir3.0 polypeptide assembled to form a functional Kir3.0 channel.
- 13. A host cell acccording to claim 12, wherein said mammalian Kir3.0 polypeptides are Kir3.1/KGA and Kir3.2.
- 14. A host cell acccording to claim 12, wherein said mammalian Kir3.0 polypeptides are Kir3.1/KGA and Kir3.3.
- 15. A host cell acccording to claim 12, wherein said mammalian Kir3.0 polypeptides are Kir3.2 and Kir3.3.
- 16. A host cell according to claim 12, wherein said host cell is a Xenopus laevis oocyte.
- 17. A method of screening for agents that modulate the activity of a Kir3.0 channel, the method comprising:
combining a candidate agent with a functional Kir3.0 channel under conditions that permit inward K+ current determining the induced current; wherein a change in said induced current in the presence of said agent as compared to a control is indicative that said agent modulates the activity of a Kir3.0 channel.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application is a continuation-in-part of U.S. patent application Ser. No. 08/066,371, filed Mar. 21, 1993.
Government Interests
[0002] The invention disclosed herein was made with U.S. Government support under USPHS grants GM29836 and MH49176. The U.S. government has certain rights in this invention.
Continuations (1)
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Number |
Date |
Country |
Parent |
08614801 |
Mar 1996 |
US |
Child |
09039927 |
Mar 1998 |
US |
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
08066371 |
May 1993 |
US |
Child |
08614801 |
Mar 1996 |
US |