Claims
- 1. An isolated nucleic acid molecule which encodes a tumor rejection antigen precursor, wherein the complementary sequence of said isolated nucleic acid molecule hybridizes to the nucleotide sequence set forth in SEQ ID NO: 20 at 0.1×SSC, 0.1%SDS.
- 2. An isolated nucleic acid molecule which encodes a fragment of a tumor rejection antigen precursor, wherein the complementary sequence of said isolated nucleic acid molecule hybridizes to the nucleotide sequence set forth in SEQ ID NO: 20 at 0.1×SSC, 0.1%SDS.
- 3. An isolated nucleic acid molecule which encodes a tumor rejection antigen, wherein the complementary sequence of said isolated nucleic acid molecule hybridizes to the nucleotide sequence set forth in SEQ ID NO: 20 at 0.1×SSC, 0.1%SDS.
- 4. An isolated cDNA molecule which encodes a tumor rejection antigen precursor, wherein the complementary sequence of said isolated nucleic acid molecule hybridizes to nucleotides 451-1156 of SEQ ID NO: 20 at 0.1×SSC, 0.1%SDS.
- 5. The isolated nucleic acid molecule of claim 1, wherein said nucleic acid molecule is cDNA.
- 6. An isolated cDNA molecule which encodes a fragment of a tumor rejection antigen precursor, wherein said fragment is processed by cell to a tumor rejection antigen, wherein the complementary sequence of said isolated nucleic acid molecule hybridizes to nucleotides 451-1156 of SEQ ID NO: 20 at 0.1×SSC, 0.1%SDS.
- 7. An isolated cDNA molecule which encodes a tumor rejection antigen, said tumor rejection antigen consisting of an amino acid sequence that is part of a tumor rejection antigen precursor, wherein said tumor rejection antigen precursor is encoded by a nucleic acid molecule the complementary sequence of which hybridizes to nucleotides 451-1156 SEQ ID NO: 20 at 0.1×SSC, 0.1% SDS.
- 8. The isolated nucleic acid molecule of claim 1, comprising SEQ ID NO: 20.
- 9. An expression vector comprising the isolated nucleic acid molecule of claim 5, operably linked to a promoter.
- 10. An expression vector comprising the isolated nucleic acid molecule of claim 6, operably linked to a promoter.
- 11. An expression vector comprising the isolated nucleic acid molecule of claim 7, operably linked to a promoter.
- 12. A host cell transformed or transfected with the isolated nucleic acid molecule of claim 1.
- 13. A host cell transformed or transfected with the isolated nucleic acid molecule of claim 2.
- 14. A host cell transformed or transfected with the isolated nucleic acid molecule of claim 3.
- 15. The host cell of claim 12, wherein said cell is a fibroblast.
- 16. The host cell of claim 13, wherein said cell is a fibroblast.
- 17. The host cell of claim 14, wherein said cell is a fibroblast.
- 18. The host cell of claim 12, wherein said cell is a mammalian cell.
- 19. The host cell of claim 13, wherein said cell is a mammalian cell.
- 20. The host cell of claim 19, wherein said cell is a mammalian cell.
- 21. An isolated nucleic acid molecule which encodes a tumor rejection antigen precursor encoded by nucleotides 451-1156 of SEQ ID NO: 20.
- 22. An isolated nucleic acid molecule which encodes a fragment of a tumor rejection antigen precursor that is encoded by nucleotides 451-1156 of SEQ ID NO: 20.
- 23. An isolated nucleic acid molecule which encodes a tumor rejection antigen, the amino acid sequence of which consists of an amino acid sequence that is a part of the amino acid sequence encoded by nucleotides 451-1156 of SEQ ID NO: 20.
- 24. The isolated nucleic acid molecule of claim 21, wherein said nucleic acid molecule is cDNA.
- 25. The isolated nucleic acid molecule of claim 22, wherein said molecule is cDNA.
- 26. The isolated nucleic acid molecule of claim 23, wherein said molecule is cDNA.
- 27. An isolated genomic DNA molecule which encodes a tumor rejection antigen precursor, and comprises a nucleotide sequence consisting of nucleotides 451-1156 of SEQ ID NO: 20.
- 28. An isolated genomic DNA molecule which encodes the tumor rejection antigen precursor encoded by the isolated genomic DNA molecule of claim 27.
- 29. The isolated genomic DNA molecule of claim 27, comprising the nucleotide sequence of SEQ ID NO: 20.
- 30. The isolated genomic DNA molecule of claim 27, consisting of the nucleotide sequence of SEQ ID NO: 20.
RELATED APPLICATION
This application is a divisional of Ser. No. 09/404,026 filed Sep. 23, 1999, which is a divisional of Ser. No. 08/967,727, filed on Nov. 12, 1997, now U.S. Pat. No. 6,025,474, which is a divisional of Ser. No. 08/037,230, filed Mar. 26, 1993, now U.S. Pat. No. 6,235,525 which is a continuation-in-part of PCT Application PCT/US92/04354 filed on May 22, 1992 designating the United States, which is a continuation-in-part of Ser. No. 07/807,043, filed Dec. 12, 1991 now U.S. Pat. No. 5,342,774, which is a continuation-in-part of Ser. No. 07/764,365, filed Sep. 23, 1991, which is a continuation-in-part of Ser. No. 07/728,838, filed Jul. 9, 1991, which is a continuation-in-part of Ser. No. 07/705,702, filed May 23, 1991, and now abandoned.
Non-Patent Literature Citations (1)
Entry |
Reiger and Green. Glossary of Genetics and Cytogenetics, Classical and Molecular, 4th Ed., Springer-Verlay, Berlin, pp. 17-18,1976. |
Continuation in Parts (5)
|
Number |
Date |
Country |
Parent |
PCT/US92/04354 |
May 1992 |
US |
Child |
08/037230 |
|
US |
Parent |
07/807043 |
Dec 1991 |
US |
Child |
PCT/US92/04354 |
|
US |
Parent |
07/764365 |
Sep 1991 |
US |
Child |
07/807043 |
|
US |
Parent |
07/728838 |
Jul 1991 |
US |
Child |
07/764365 |
|
US |
Parent |
07/705702 |
May 1991 |
US |
Child |
07/728838 |
|
US |