Claims
- 1. Isolated nucleic acid molecule which encodes a mammalian .alpha.2,8 polysialyl transferase.
- 2. The isolated nucleic acid molecule of claim 1, wherein said .alpha.2,8 polysialyl transferase is a rodent .alpha.2,8 polysialyl transferase.
- 3. The isolated nucleic acid molecule of claim 1, wherein said .alpha.2,8 polysialyl transferase is a human .alpha.2,8 polvsialyl transferase.
- 4. The isolated nucleic acid molecule of claim 1, consisting of the nucleotide sequence of SEQ ID NO: 1.
- 5. The isolated nucleic acid molecule of claim 1, consisting of the nucleotide sequence of SEQ ID NO: 7.
- 6. The isolated nucleic acid molecule of claim 1, wherein said molecule encodes a protein having the amino acid sequence set forth in SEQ ID NO: 2.
- 7. The isolated nucleic acid molecule of claim 1, wherein said molecule encodes a protein having the amino acid sequence set forth in SEQ ID NO: 8.
- 8. The isolated nucleic acid molecule of claim 6, wherein said isolated nucleic acid molecule consists of nucleotides 301-1377 of SEQ ID NO: 1.
- 9. The isolated nucleic acid molecule of claim 7, wherein said isolated nucleic acid molecule consists of nucleotides 213-1289 of SEQ ID NO: 7.
- 10. The isolated nucleic acid molecule of claim 1, the complementary sequence of which hybridizes to nucleotides 301-1277 of SEQ ID NO: 1 under stringent conditions.
- 11. The isolated nucleic acid molecule of claim 1, the complementary sequence of which hybridizes to nucleotides 213-1289 of SEQ ID NO: 7 under stringent conditions.
- 12. The isolated nucleic acid molecule of claim 1, wherein said .alpha.2,8 polysialyl transferase is a soluble .alpha.2,8 polysialyl transferase.
- 13. The isolated nucleic acid molecule of claim 12, wherein said soluble .alpha.2,8 polysialyl transferase consists of: amino acids 21-359 of SEQ ID NO: 2; amino acids 26-359 of SEQ ID NO: 2; amino acids 31-359 of SEQ ID NO: 2; amino acids 21-359 of SEQ ID NO: 8; amino acids 26-359 of SEQ ID NO: 8; amino acids 31-359 of SEQ ID NO: 8, and amino acids 40-359 of SEQ ID NO: 8.
- 14. Isolated cell line or cell strain transformed or transfected with the isolated nucleic acid molecule of claim 1.
- 15. The isolated cell line of claim 14, wherein said cell line is eukaryotic.
- 16. Expression vector comprising the isolated nucleic acid molecule of claim 1, operably linked to a promoter.
- 17. The expression vector of claim 16, comprising SEQ ID NO: 1.
- 18. The expression vector of claim 16, comprising SEQ ID NO: 7.
- 19. The expression vector of claim 16, comprising nucleotides 301-1377 of SEQ ID NO: 1 or nucleotides 213-1289 of SEQ ID NO: 7.
Government Interests
Funds from NIH Grant CA 33895 were used in the development of portions of the invention described herein. Thus the U.S. government may have rights to portions of this invention.
Non-Patent Literature Citations (3)
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