Claims
- 1. A method of assaying a candidate ligand compound for binding affinity to a human EAA receptor, which comprises the steps of incubating a labeled form of said compound with a cell or with a membrane preparation derived from said cell, said cell having incorporated expressibly therein a heterologous DNA molecule that encodes a human EAA2 receptor, having the amino acid sequence of residues 1-962 of SEQ ID NO:2, or a kainate-binding variant of said EAA2 receptor which is at least 95% homologous to said EAA2 receptor, washing unbound ligand compound from the incubation mixture, and then determining the presence of membrane-bound ligand compound.
- 2. A method for determining the binding affinity of a candidate ligand compound for a human EAA receptor, which comprises the steps of incubating a cell or a membrane preparation derived from said cell with a labeled EAA receptor ligand to form a ligand/receptor complex, said cell having incorporated expressibly therein a heterologous DNA molecule that encodes a human EAA2 receptor having the amino acid sequence of residues 1-962 of SEQ ID NO:2 or a kainate-binding variant of said EAA2 receptor which is at least 95% homologous to said EAA2 receptor, removing unbound ligand, incubating the receptor/ligand complex with said compound, and measuring the amount of labeled ligand displaced from or remaining in the receptor/ligand complex.
Parent Case Info
This application is a division of application Ser. No. 07/750,081, filed Aug. 27, 1991, now abandoned.
Non-Patent Literature Citations (5)
Entry |
Egebjerg, J. et al. Nature 351:745-749 (1991). |
Wada, K. et al. Nature 342:684-689 (1989). |
Werner, P. et al. Nature 351:742-744 (1991). |
Bochet, P. et al. in Comparative Molecular Neurobiology, ed. by Y. Pideon, pp. 224-231. (1993). |
Bowie, D. et al., British Journal of Pharmacology 111(3):803-810 (1994). |
Divisions (1)
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Number |
Date |
Country |
Parent |
750081 |
Aug 1991 |
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