Claims
- 1. An isolated polynucleotide segment encoding an amino acid sequence as set forth in SEQ ID NO:2.
- 2. An isolated polynucleotide segment selected from the group consisting of:a) SEQ ID NO:1; b) SEQ ID NO:1, wherein each of the T residues is a U; and c) nucleic acid sequences fully complementary to SEQ ID NO:1.
- 3. An expression vector containing the polynucleotide of claim 1.
- 4. The expression vector of claim 3, wherein the vector is a plasmid.
- 5. The expression vector of claim 3, wherein the vector is a viral vector.
- 6. The polynucleotide of claim 1, wherein the polynucleotide sequence is from Leptospira kirschneri.
- 7. A host cell transformed with an expression vector of claim 3.
- 8. The host cell of claim 7, wherein the cell is a eukaryotic cell.
- 9. The host cell of claim 7, wherein the cell is a prokaryotic cell.
- 10. A method of producing LipL32 polypeptide comprising:a) transforming a host cell with a polynucleotide of claim 1; b) expressing the polynucleotide in the host; and c) recovering the LipL32 polypeptide.
- 11. The method of claim 10, wherein the host cell is a prokaryotic cell.
- 12. A kit useful for the detection of a LipL32 nucleic acid, the kit comprising one or more containers comprising a first container containing a polynucleotide selected from the group consisting of:a) SEQ ID NO:1; b) SEQ ID NO:1, wherein each of the T residues is a U; and c) nucleic acid sequences fully complementary to SEQ ID NO:1.
- 13. An isolated polynucleotide segment encoding an amino acid sequence as set forth in SEQ ID NO:2 operably linked to a heterologous nucleic acid sequence.
- 14. The isolated polynucleotide of claim 13, wherein the heterologous nucleic acid sequence is a coding region for a polypeptide.
- 15. The isolated polynucleotide of claim 13, wherein the heterologous nucleic acid sequence is a regulatory element.
- 16. The isolated polynucleotide of claim 15, wherein the regulatory element is a promoter.
Government Interests
This invention was made with Government support under Grant No. AI-34431, awarded by NIH. The Government has certain rights to this invention.
US Referenced Citations (3)
Number |
Name |
Date |
Kind |
5178859 |
Simon et al. |
Jan 1993 |
|
5302527 |
Birkett et al. |
Apr 1994 |
|
6068843 |
Duhamel et al. |
May 2000 |
|
Foreign Referenced Citations (1)
Number |
Date |
Country |
WO 9532220 |
Nov 1995 |
WO |
Non-Patent Literature Citations (1)
Entry |
Wilson et al (Nature vol. 368, pp, 32-38) Mar. 3, 1994. |