Patent Application
20050175746
The presently disclosed subject matter relates to methods and compositions for anti-microbial treatment of food products. More particularly, the presently disclosed subject matter relates to derivatives of liquid smoke and methods of treating food products with the derivatives to inhibit the growth of microorganisms without imparting smoky flavors to the food product.
The presence of microorganisms on food products is a considerable public health concern. For example, outbreaks of food-borne illness and death associated with various strains of Escherichia coli are frequently reported. In fact, E. coli 0157: H7 is a recognized food-borne pathogen that has been found to contaminate undercooked beef products, especially ground beef.
Another such food-borne pathogen is Listeria monocytogenes, which can cause pneumonia, meningitis, and sepsis. The incidence of Listeria monocytogenes in the meat processing industry has caused great concern to packers, and it is believed to be a major current health threat. The presence of Listeria monocytogenes bacteria on processed meat products represents a danger to the public in that the bacteria can grow on food and increase to an infectious dose under refrigerated conditions.
Given the importance of maintaining a safe food supply, various approaches have been taken in attempts to kill pathogens that might be present in or on food. One traditional approach is to smoke the food product in a smokehouse. This is, however, a time consuming process requiring large amounts of space, and thus more efficient approaches are desirable.
Currently, the treatment of foods with wood smoke has been substantially replaced by the use of “liquid smoke”, which is a solution comprising liquid condensates capable of imparting smoky hue or coloration and smoky flavor to a meat exposed to a liquid or vapor phase of the solution. In addition to these attributes of liquid smoke, it is also now known that liquid smoke preparations that are applied in the manufacturing of meat products have antimicrobial activity against bacteria such as Listeria monocytogenes.
Unfortunately, although liquid smoke application is probably quite effective during meat processing to kill Listeria monocytogenes bacteria, it has recently become apparent that there is a danger that re-inoculation or re-contamination by Listeria monocytogenes bacteria can occur at some point between cooking and final packaging during the meat processing sequence. Unfortunately, even very low levels of bacterial contamination due to re-inoculation can result in dangerously high levels of bacteria by the time the meat product has been on the store shelf for a period of time.
In an effort to address this problem, research has been conducted by the meat processing industry into the use of an application of liquid smoke directly to the meat product prior to packaging in order to prevent re-inoculation and subsequent growth of Listeria monocytogenes bacteria on the packaged meat product. See U.S. Pat. No. 5.043,174 to Lindner. Thus, the bacteria is initially killed or minimized by the liquid smoke and heat treatment that takes place during the meat processing cycle, and prior to packaging, another treatment of liquid smoke is applied directly to the surface of the processed food product to control the re-inoculation of the meat product with bacteria or other microorganisms.
While simple and efficient, this approach is not always acceptable, however, since meat products having an application of liquid smoke applied thereto subsequent to the conventional meat packaging process are adversely affected with respect to taste. The additional liquid smoke results in an undesirable over-enhancement of the smoky flavor of the meat product and a generally commercially unsatisfactory product. More importantly, treatment of a food product with liquid smoke would be particularly undesirable with foods that are not intended to have a smoky flavor at all. Thus, the search has continued for a solution to the problem of growth on food products of dangerous pathogens that does not render the meat product substantially inedible and consequently unmarketable.
What is needed, then, is a derivative of liquid smoke that retains anti-microbial activity but does not impart smoky flavors to the food product. To meet this need, the presently disclosed subject matter provides derivatives of liquid smoke and methods of treating food products with derivatives of liquid smoke to inhibit the growth of microorganisms without altering the flavor of the food product.
The presently disclosed subject matter provides methods for inhibiting growth of a microorganism in a food product. In some embodiments, the method comprises treating the food product with a derivative of liquid smoke that imparts no smoke flavoring to the food product, whereby growth of a microorganism is inhibited. In some embodiments, the microorganism is selected from the group consisting of a bacterium, a yeast, and a fungus. In some embodiments, the bacterium is selected from the group consisting of a strain of Streptococcus, Shigella, Hafnia, Enterobacter, Serratia, Staphylococcus, Pseudomonas, Citrobacter, Klebsiella, Escherichia coli, Listeria, and Salmonella. In some embodiments, the yeast is a strain of Saccharomyces. In some embodiments, the fungus is a strain of Aspergillus.
In some embodiments of the presently disclosed subject matter, the food product is a ready-to-eat (RTE) food product. In some embodiments, the ready-to-eat food product comprises poultry, pork, or beef. In some embodiments, RTE foods include deli meats (e.g. turkey, roast beef, ham, chicken, salami, bologna, etc.), and hot dogs.
In some embodiments of the presently disclosed subject matter, the food product is a ready-to-cook (RTC) food product. In some embodiments, the ready-to-cook food product comprises poultry, pork, beef or a par-baked dough product. In some embodiments, the ready-to-cook food product comprises ground beef, or par-baked dough products such as bread and rolls.
In some embodiments of the presently disclosed subject matter, the derivative of liquid smoke comprises: (a) titratable acidity in a concentration of 0 to about 6% weight per unit volume (w/v); (b) at least about 3% weight per unit volume (w/v) carbonyl; (c) phenol in a concentration of less than about 0.5% weight per unit volume (w/v); and (d) water in a concentration of less than about 97% weight per unit volume (w/v). In some embodiments, the derivative of liquid smoke comprises carbonyl in a concentration of about 8.0 to about 12.0% weight per unit volume (w/v), and a pH of about 5.0 to about 6.0. In some embodiments, the derivative of liquid smoke comprises carbonyl in a concentration of about 3.0 to about 8.0% weight per unit volume (w/v), phenol in a concentration of about 0.01 to 0.5% weight per unit volume (w/v), and a pH of about 5.0 to about 6.0. In some embodiments, the derivative of liquid smoke has a pH of at least about 3.0. In some embodiments, the pH is between about 4.5 and 6.5. In some embodiments, the derivative of liquid smoke comprises carbonyl of at least 10% weight per unit volume (w/v).
In some embodiments, one derivative of liquid smoke is produced by processing liquid smoke through an evaporator to separate and condense the low boiling elements thereof to produce the derivative of liquid smoke. In some embodiments, the sawdust is delignified prior to pyrolysis to produce low flavor products. Various of these products and derivatives can be blended together to produce a range of carbonyl levels. They can be carbon treated to substantially reduce phenols. They can also be treated with neutralizing agents to adjust pH/acidity. In some embodiments, the derivative of liquid smoke is sprayed onto the food product. In some embodiments, the food product is dipped into a bath of the derivative of liquid smoke. In some embodiments, the derivative of liquid smoke comprises an additional welting agent. In some embodiments, the additional wetting agent comprises polysorbate.
In some embodiments, the presently disclosed subject matter further comprises heating the food product to at least about 165° F. for at least about 1 minute. In some embodiments, the heating step is performed after the treating step.
The presently disclosed subject matter also provides antimicrobial derivatives of liquid smoke. In some embodiments, the derivative of liquid smoke: (i) comprises carbonyl of at least 3% weight per unit volume (w/v); (ii) imparts no liquid smoke flavor to a food product when the food product is treated with the derivative of liquid smoke; and (iii) inhibits growth on a food product of a microorganism selected from the group consisting of Streptococcus, Shigella, Hafnia, Enterobacter, Serratia, Staphylococcus, Pseudomonas, Citrobacter, Klebsiella, Eschetichia col, Listeria, Salmonella, Saccharomyces, and Aspergillus when the food product is treated with the derivative of liquid smoke. In some embodiments, the derivative of liquid smoke has a pH of about 3.0 or more. In some embodiments, the pH is between about 4.5 and 6.5. In some embodiments, the derivative of liquid smoke comprises carbonyl of at least 10% weight per unit volume (w/v).
In some embodiments, the derivative of liquid smoke comprises: (a) titratable acidity in a concentration of 0 to about 6% weight per unit volume (w/v); (b) at least about 3% weight per unit volume (w/v) carbonyl; (c) phenol in a concentration of less than about 0.5% weight per unit volume (w/v); and (d) water in a concentration of less than about 97% weight per unit volume (w/v). In some embodiments, the derivative of liquid smoke comprises carbonyl in a concentration of about 8.0 to about 12.0% weight per unit volume (w/v) and a pH of about 5.0 to about 6.0. In some embodiments, the derivative of liquid smoke comprises carbonyl in a concentration of about 5.0 to about 8.0% weight per unit volume (w/v), phenolics in a concentration of about 0.01 to 0.5% weight per unit volume (w/v), and a pH of about 5.0 to about 6.0.
In some embodiments of the presently disclosed subject matter, the food product is a ready-to-eat food product.
In some embodiments, the ready-to-eat food product comprises poultry, pork, beef, or a baked dough product. In some embodiments, RTE foods include deli meats (e.g. turkey, roast beef, ham, chicken, salami, bologna, etc.) or hot dogs.
In some embodiments, the food product is a ready-to-cook food product. In some embodiments, the ready-to-cook food product comprises poultry, pork, or beef. In some embodiments, the ready-to-cook food product comprises ground beef. In some embodiments, the RTC food comprises par-baked dough products such as bread and rolls.
In some embodiments, the derivative of liquid smoke comprises an additional wetting agent. In some embodiments, the additional wetting agent comprises polysorbate.
An object of the presently disclosed subject matter having been stated above, other objects and advantages of the presently disclosed subject matter will become apparent to those of ordinary skill in the art after a study of the following description and non-limiting Examples.
All references cited in the specification, including patents, patent application publications, and non-patent literature, are incorporated herein by reference to the extent that they supplement, explain, provide a background for, or teach methodology, techniques, and/or compositions employed herein. Also included by reference are the following U.S. Patents to Moeller and/or Moeller et al.: U.S. Pat. Nos. 5,637,339; 6,214,395; 6,261,623; 6,541,053.
I. Definitions
All technical and scientific terms used herein, unless otherwise defined below, are intended to have the same meaning as commonly understood by one of ordinary skill in the art. References to techniques employed herein are intended to refer to the techniques as commonly understood in the art, including variations on those techniques or substitutions of equivalent techniques that would be apparent to one of skill in the art. While the following terms are believed to be well understood by one of ordinary skill in the art, the following definitions are set forth to facilitate explanation of the presently disclosed subject matter.
Following long-standing patent law convention, the terms “a”, “an” and “the” mean “one or more” when used in this application, including the claims.
As used herein, unless specifically indicated otherwise, the word “or” is used in the “inclusive” sense of “and/or” and not the “exclusive” sense of “either/or”.
As used herein, the phrase “liquid smoke (LS)” refers to a solution comprising liquid reagents capable of imparting a smoky hue or coloration and smoky flavor to a food product exposed to a liquid or vapor phase of the solution. The use of liquid smoke provides many advantages in meat processing including the ability to use continuous processing when smoking meat as well as more uniform smoke taste and smoke coloration to the meat products treated therewith. The use of liquid smoke in lieu of wood smoke is now conventional in meat processing and can be more fully appreciated with reference to representative U.S. Pat. Nos. 3,873,741; 4,250,199; 4,298,435; and 5,043,174.
LS has also been found to possess antimicrobial activity. For example, U.S. Pat. No.5,043,174 discloses that treatment of hot dogs with LS (ZESTI-SMOKE (Code 10), available from Mastertaste of Crossville, Tenn., United States of America) can prevent post-processing re-inoculation with Listeria monocytogenes. However, the LS used imparts significant smoky flavor on the treated food, which can be undesirable under certain circumstances. Thus, it would be advantageous to generate derivatives of liquid smoke that retain antimicrobial activity yet impart no smoky flavor to food products during the treatment stage. That is the unexpected and surprising result of the methods and compositions discovered and described and claimed herein.
As used herein, the phrase “antimicrobial activity” refers generally to an activity of a liquid smoke or derivative of liquid smoke that results in either the killing of a microorganism (including, but not limited to microbicidal and microbiolytic activities) or the inhibition of the growth of a microorganism (including, but not limited to a microbiostatic activity). With respect to an inhibition of microbial growth, the term “antimicrobial activity” is intended to encompass both total inhibition (i.e. the microorganism does not grow at all or at an undetectable rate in the presence of the DLS) and partial inhibition, the latter being characterized by either a delay in the initiation of growth of the microorganism or a reduction in the rate at which the microorganism grows, or both.
As used herein, the phrase “derivative of liquid smoke (DLS)” refers to a derivative of liquid smoke that has characteristics that are appropriate for a given use. DLSs are typically fractions of a liquid smoke that are prepared, for example, by processing a conventional liquid smoke through an evaporator, which separates and condenses the low boiling elements of the liquid smoke to produce the derivative of liquid smoke solution. Accordingly, the phrases “derivative of liquid smoke”, “derivative”, “liquid smoke fraction”, and “fraction” are used interchangeably herein and refer to a component of a liquid smoke that has been isolated from the liquid smoke itself, either with or without subsequent additional preparation and/or modification steps. In some embodiments, a DLS is characterized by antimicrobial activity and does not impart smoky flavors to a food product when the food product is treated with the DLS.
As used herein, the phrase “ready-to-eat (RTE)” refers to food products that are prepared such that they can be consumed either immediately or after re-heating. Exemplary RTE foods include deli meats (e.g. turkey, roast beef, ham, chicken, salami, bologna, etc.), and hot dogs.
RTE food products can be contrasted with ready-to-cook food products. Ready-to-cook food products typically include raw, uncooked foodstuffs such as poultry, pork, and beef, and partially cooked/baked foods such as par-baked dough products. Exemplary ready-to-cook food products are poultry, pork, and beef (e.g. ground beef) and par-baked dough products such as bread and rolls. RTC food products can also include seafood, vegetables, and other minimally processed foods.
II. Preparation of Liquid Smoke Derivatives from Liquid Smoke
As is well known to those skilled in the art, liquid smoke compositions obtained from pyrolysis of hardwood sawdust contain constituents primarily from the thermal degradation of cellulose, hemicellulose, and lignin. More particularly, the liquid smoke compositions contain a wide array of over 400 chemical compounds, and hence, liquid smoke compositions are characterized by their content of certain classes of compounds, namely, acids (% titratable acidity, determined using the method disclosed in U.S. Pat. No. 6,214,395), phenolics, and carbonyls.
The acids are preservatives and pH control agents. Commercial liquid smoke compositions typically have a pH under about 2.5, and more typically under about 2.3, and a % titratable acidity by volume from about 3% to about 18%. The phenolics give a smoky flavor, and also aroma, to liquid smoke compositions, which typically have a phenolics content from about 10 to about 45, and more typically, from about 14 to about 30 mg/ml. The carbonyls impart the brown color-forming capacity to liquid smoke compositions. The phenolics and the carbonyls can be measured as described in U.S. Pat. No. 4,431,032, which describes techniques for the removal of an undesirable tar component from liquid smoke compositions. It is noted that the acids and carbonyls are secondary in contributing to the smoky flavor of liquid smoke compositions.
Mastertaste of Crossville, Tenn. is a manufacturer of various liquid smokes. Exemplary liquid smokes include ZESTI-SMOKE Code 10 and ZESTI-SMOKE Code V. The specifications of these liquid smokes are as follows:
The ZESTI-SMOKE Code V fraction utilized as a starting material by the presently disclosed subject matter can be produced as a derivative or secondary product of ZESTI-SMOKE Code 10. The Code 10 can be processed through a separator (for example, an AVP evaporator) by feeding the Code 10 as a feed stock which is first heated to remove low boiling acids from the top of the evaporator and then is condensed into Code V as a secondary product. This process also yields a concentrated liquid smoke that has higher percent acid, staining index, carbonyl and phenolic levels, specific gravity, density, and darker color than conventional liquid smoke, and that is sold under the trademark SUPERSMOKE™ by Mastertaste of Crossville, Tenn. for a variety of end uses.
The Code V derivative is a low pH, low flavor, low or no stain product. In some embodiments, Code V is then treated with a suitable pH adjustment agent, such as sodium bicarbonate, sodium carbonate, sodium hydroxide, or potassium hydroxide, in order to bring the pH up to at least about 5.0. The pH can be brought up to as high as about 7.0. In some embodiments, the pH ranges from about 5.0 to about 6.0. This pH-adjusted material can be further modified to produce a derivative of liquid smoke as disclosed herein.
In some embodiments, the Code V derivative is first treated with carbon in accordance with the method disclosed in U.S. Pat. No. 5,637,339 to Moeller. This removes phenolics. The resultant is then treated with the suitable pH adjustment agent. Optionally, the pH adjustment can be performed prior to the carbon treatment. This carbon treated, pH adjusted material can also be used as a starting material for producing a derivative of liquid smoke as used herein.
In some embodiments, the sawdust is delignified prior to pyrolysis to produce low flavor products. Various of these products and derivatives can be blended together to produce a range of carbonyl levels. They can be carbon treated to substantially reduce phenolics. They can also be treated with neutralizing agents to adjust pH/acidity.
In the Examples presented below, various derivatives of liquid smoke were used to test the antimicrobial activity of DLSs versus bacteria, yeast, and mold.
The following Examples have been included to illustrate modes of the presently disclosed subject matter. Certain aspects of the following Examples are described in terms of techniques and procedures found or contemplated by the present inventors to work well in the practice of the presently disclosed subject matter. These Examples illustrate standard practices of the inventors. In light of the present disclosure and the general level of skill in the art, those of skill will appreciate that the following Examples are intended to be exemplary only and that numerous changes, modifications, and alterations can be employed without departing from the scope of the presently disclosed subject matter.
Smokes 1, 2, and 5 were derived from primary condensates of smokes that had been substantially stripped of their phenolic content by various combinations of delignification and dephenolization as described herein. Smoke 3 was a standard version of a primary smoke condensate having the full complement of acids, carbonyls, and phenolics. Its strength was adjusted by dilution to have a titratable acidity similar to some of the other LS/DLS fractions for easier comparison. Smoke 4 was an extract of the insoluble tar fraction that settled out of a typical primary smoke condensate. It was predominantly a high phenolic fraction with lesser amounts of titratable acidity and carbonyl compounds. It was rendered water-soluble by the addition of Polysorbate 80. The preparation of Smokes 6, 7, 8, and 9 involved various manipulations of the condensate from evaporation of a primary LS. These manipulations adjusted the products to have varying levels of titratable acidity, pH, and phenolics with little or no impact on carbonyl compositions.
A total of nine derivatives of liquid smoke were generated with the characteristics summarized in Table 1:
*Quantified as acetic acid
**Quantified as 2,6-dimethoxy phenol
***Quantified as 2-butanone
Derivatives of liquid smoke 1-9 from Example 1 were used in broth or agar dilution methods against a cocktail of Gram-negative bacteria. The cocktail included Salmonella muenster, Salmonella seftenberg, Salmonella typhimurium and E. coli 8677. 1000 cells of each bacterial species were used to inoculate various dilutions (dilutions are presented as v/v%) of the smoke fractions.
In order to determine the minimum inhibitory concentrations for each of the fractions, appropriate inoculations were then carried out to TSB containing different percentages of derivatives of liquid smoke in a test tube. The test tubes were incubated at 37° C. for 24 and 48 hours and scored for growth/no growth. The MIC values were conducted in triplicate and repeated three times for each. The MIC values are presented in Table 2.
Growth curves were constructed below the predetermined MIC levels for selected derivatives of liquid smoke (DLS) for several bacterial strains individually. The growth curves presented in FIGS. 14 each represents the average of three replicates.
Derivatives of liquid smoke 1-9 were also tested against a Gram-positive bacterium (Listeria innocua Ml) using the techniques described in Example 2. The MIC values are presented in Table 3.
Growth curves were constructed below the predetermined MIC levels for selected derivatives of liquid smoke (DLS) for Listeria innocua M1. The growth curves presented in
Derivatives of liquid smoke (DLSs) 1, 2, 3, and 8 were also tested against Saccharomyces cerevisiae using the techniques described in Example 2, except that malt extract broth (MEB) was used instead of TSB. The MIC values were 1.5% for each DLS.
Growth curves were constructed for DLSs 1, 2, 3, and 8 at 0.50%, and for DLS 1 at 0.25%, 0.5%, and 0.75%, for Saccharomyces cerevisiae. The growth curves presented in
Derivatives of liquid smoke 1, 2, 3, and 8 were also tested against Aspergillus niger using the techniques described in Example 2, except that instead of inoculating 1000 cells to each DLS dilution, an equal volume of A. niger spores was added to potato dextrose agar (PDA). The MIC values are presented in Table 4.
As a proxy for growth curves, the circumference of A. nigerspores was measured at 37° C. for DLS 1 at 0.75%, DLS 2 at 1.25%, DLS 3 at 1.25%, and DLS 8 at 5.0%, at days 1, 3, 4, and 7 of treatment. The data are presented in
As is disclosed hereinabove and in the Figures, components of smoke possess antimicrobial properties. While different smoke condensates behave somewhat differently against different microorganisms, with some exceptions, the data presented suggest a general correlation between acidity and pH on MIC values. Interestingly, these data also suggest that carbonyls contribute to the antimicrobial efficacy against Gram-negative bacteria, Gram-positive bacteria, yeast, and fungi. MIC values may also be influenced by the compensation of one variable over another (e.g. carbonyls vs. phenolics, and vice versa).
Par-baked dinner rolls were purchased two days before the expiration date on the package. On the day before expiration, they were sprayed lightly with a 30% solution of Smoke 1, packaged, and held at room temperature. Pick-up level was 1.5% to 2% to ensure coverage. Rolls were observed beginning 24 hours after treatment (i.e. on the expiration date).
The results are depicted in
RTE high end (whole breast parts formed to have no more than 40% binders and broth added) and low end (minced turkey breast parts that can have up to 60% binders and broth added before forming and cooking) turkey rolls and roast beef cuts from a commercial manufacturer were used to test the ability of smoke fractions to control L. innocua M1 infection over the course of 4 weeks. The turkey products ranged from 3.5 to 4.5 kg and were cooked in hot water in shrinkable cook-in bags to an internal temperature of 71° C. followed by cooling in water to an internal temperature of 7° C. Each piece of the roast beef was about 1 kg and was in case-ready shrink film packages, and was used as a unit. The turkey rolls were each cut into 4 sections, with each section considered a unit.
Four different derivatives of liquid smoke (DLSs) were obtained from Mastertaste of Crossville, Tenn., United States of America. Each meat was dipped in 100% DLS and remained submerged for at least 60 seconds. After removal, the meats were allowed to air dry over a screen at room temperature for no less than 5 minutes before they were inoculated with L. innocua M1 (obtained from Dr. P. M. Foegeding, North Carolina State University, Raleigh, N.C., United States of America).
For each piece of meat, two 25 cm2 areas on the surface (marked with food grade ink using a sterile template) were inoculated with 100 CFU of L. innocua M1 in 50 μL from an actively growing (18 hour) culture to obtain a total inoculum of 100 CFU/25 cm2. Each piece was then placed in a CRYOVAC® barrier bag (available from CRYOVAC® Food Packaging of Duncan, S.C., United States of America), vacuum-sealed, and placed at 4° C.
Viable L. innocua M1 were evaluated at 0, 2, and 4 weeks at 4° C. The marked area was aseptically excised and transferred to a stomacher bag. 100 mL of 0.1% sterile peptone water (PW) was added and the mixture was stomached for 2 minutes. Aliquots were removed and directly plated or dilutions made and enumerated. Enumeration of viable L. innocua M1 cells was done by direct spiral plating methods on an AUTOPLATE® 4000 (available from Spiral Biotech, Inc. of Norwood, Mass., United States of America) using DIFCO® TSA (available from Difco Laboratories, Inc. of Detroit, Mich., United States of America) supplemented with 250 mg/L of streptomycin and 50 mg/mL rifampicin. There were three batches of each RTE product (high end and low end turkey, and roast beef cut) with 15 tests conducted on each batch. Three samples from each batch served as positive controls (no DLS treatment), one for each sampling time. Data are presented as an average of CFU from the two designated areas on each product and two samples of purge in Tables 5-7.
* Indicates a positive identification of Listeria innocua M1 after enrichment as per the United States Department of Agriculture's Section 36.512 enrichment procedure for isolation of L. monocytogenes. All other samples tested were negative after enrichment (tests not performed at time=0 weeks). ND: not detectable (less than 10 CFU/mL).
*Indicates a positive identification of Listeria innocua M1 after enrichment as per the United States Department of Agriculture's Section 36.512 enrichment procedure for isolation of L. monocytogenes. All other tests were negative after enrichment.
—: not determined (no purge at day 0);
ND: not detectable (less than 10 CFU/mL).
*Indicates a positive identification of Listeria innocua M1 after enrichment as per the United States Department of Agriculture's Section 36.512 enrichment procedure for isolation of L. monocytogenes. All other tests were negative after enrichment.
ND: not detectable (less than 10 CFU/mL).
A bacterial composite containing equal numbers of three strains of Listeria monocytogenes (SLR10, ½a; SLR31, ½b; and SLR1234, 4b ScottA; available from Silliker, Inc. of South Holland, Ill., United States of America) was employed to test the antimicrobial activity of DLSs Smoke 8 and Smoke 2 on food products. Approximately 1000-10,000 CFU were inoculated onto ham, roast beef, hot dogs, and turkey samples. The inoculum was spread over the surface of the food products using a sterile loop, and allowed to dry for 15 minutes. Individual pieces of each deli style meat product were dipped into Smoke 8 or Smoke 2 for 15 seconds and allowed to drip for 1 minute to remove excess liquid. The products were then packaged in a heat-sealed packaging, and stored at 4° C. Individual samples were analyzed at days 0, 1, 2, 5, 10, 30, 60, 90, and/or 120 days. Both the meat itself and any exudate will be tested. The data presented in FIGS. 15-18 represent the average of three replicates at each time point.
As shown in
As shown in
Smoke 8 and Smoke 2 also inhibited the growth of Listeria on ham. As shown in
Summarily, treatment of these RTE food products with DLS fractions Smoke 8 and Smoke 2 resulted in an increased shelf life of at least 10 days.
The experiments described in Example 8 were repeated, but this time the initial inoculation was between 105 and 107 CFU for each food product. The test products were inoculated with Listeria monocytogenes. The Listeria cocktail was made by combining equal portions of five USDA-recognized Listeria strains that were 12 to 18 hours old. To inoculate the test products, 0.1 ml of the Listeria cocktail was placed on the products with a micropipetor. The data presented in
Hot dogs purchased from a commercial source were treated with 100% liquid smoke (Smoke 2; Mastertaste) and/or steam pasteurized (1 minute at 1650° F.). Hot dogs were dipped in Smoke 2 for 2 minutes and allowed to drip for 60 seconds to remove excess liquid. A second set of control hot dogs was set aside without treatment. Then they were inoculated with a four-strain cocktail of Listeria monocytogenes. Samples were vacuum-packaged in suitable plastic films. A portion of the hotdogs from each set (treated and untreated) was subjected to a heat pasteurization step (1 minute at 165° F.). Trials were done in triplicate and samples were held at 50° F. (abuse temperature). The results presented in
The combination treatment with both liquid smoke and heat had an initial effect that was similar to that seen with heat alone. However, the liquid smoke treatment prevented the rapid recovery of the bacterium seen with heat alone. Additionally, the bacterial titer continued to diminish through about 3 weeks, reaching a final reduction of about 7 logs and remained at that level for the duration of the test period.
Materials and Methods for Example 11
Inoculum Preparation. A four-strain cocktail of Listeria monocytogenes (109, 108M, serotype 4c, serotype 3) was used for surface inoculation of restructured, skinless turkey breast. Inoculum was prepared from slants after two consecutive transfers to TSB bottles (available from Difco Laboratories, Inc.) in 5 mL tubes and subsequently to 100 mL TSB centrifugation bottles. Stationary phase cultures (20 hours) were centrifuged at 10,000 g in a Beckman J2-21 M/E centrifuge using a JA-14 rotor (available from Beckman Coulter Inc. of Fullerton, Calif., United States of America) at 4° C. for 10 minutes, washed with sterile 0.1% peptone water (PW), and the four strains (about 109 CFU/mL) were combined in equal volumes (50 mL each) and used for mist inoculation. Inoculum levels were determined by direct plating on Modified Oxford Agar (MOX, available from Oxoid Ltd., of Basingstoke, Hampshire, England) using a Whitley spiral plater (available from Don Whitley Scientific Ltd., of Shipley, West Yorkshire, England) and incubated at 37° C. for 24 hours. After incubation, typical black colonies were count and recorded as logio CFU/mL for inoculum level.
Product Inoculation and Treatment. The product casings were removed aseptically, and the products were placed on a tray and mist inoculated with the four strain cocktail in a bio-containment chamber. The products were held for 15 minutes to allow attachment of the L. monocytogenes. Treatments requiring application of DLSs were sprayed with the DLSs (50 mL/turkey breast) using a garden sprayer. The inoculated, treated product was then vacuum packaged and pasteurized at the Kansas State University Aseptic Processing Laboratory (Manhattan, Kans., United States of America) in a Townsend post-process pasteurizer (available from Townsend of Des Moines, Iowa, United States of America) at 96° C. for either 1, 2, or 3 minutes. Pasteurized products were then chilled in an ice water bath for 15 minutes and sampled using a surface-coring device.
Sampling for residual L. monocyto genes. After pasteurization and chilling, the inoculated product was sampled by aseptically removing the product from the package and coring both the top and the bottom surfaces (2 per side). The surface core samples were then mixed with 50 mL of 0.1% PW in a stomacher bag and homogenized (Tekmar Co. of Cincinnati, Ohio, United States of America) for 2 minutes. The homogenized samples were then serially diluted in 0. 1% PW and plated onto MOX and TSA. The plates were incubated at 37° C. for 245 hours. Enumeration was performed by counting typical black colonies on MOX and TSA (used for recovery of heat injured cells). The counts were reported as log10 CFU/cm2.
To evaluate the destruction of surface inoculated Listeria monocytogenes by DLS alone or in combination with saturated steam, a three-by-four experimental design was employed. Three DLS treatments (control, Smoke 1, and Smoke 2) were used, and four heat treatments (0, 1, 2, and 3 minutes) as described in the section above entitled “Materials and Methods for Example 11”.
Spray inoculation of restructured turkey breast products resulted in surface L. monocytogenes populations of 4.17 log10 CFU/cm2 (
While surface spray treatment of processed turkey breast product resulted in reductions of L. monocytogenes, the combination of surface smoke application and surface heat treatment provided greater reductions than either treatment alone (
It will be understood that various details of the presently disclosed subject matter can be changed without departing from the scope of the presently disclosed subject matter. Furthermore, the foregoing description is for the purpose of illustration only, and not for the purpose of limitation.
This application claims the benefit of U.S. Provisional Patent Application Ser. No. 60/536,160, filed Jan. 13, 2004, the disclosure of which is incorporated herein by reference in its entirety.
| Number | Date | Country | |
|---|---|---|---|
| 60536160 | Jan 2004 | US |
