Claims
- 1. An isolated and purified protein which is a human augmenter of liver regeneration, said protein having the property of specifically stimulating the growth of liver cells.
- 2. The protein of claim 1, which is further characterized by being purified from human liver.
- 3. The protein of claim 1, whose activity can be detected in dog portacaval shunt, partially hepatectomized rat and partially hepatectomized mouse assays.
- 4. The protein of claim 1, which is further characterized by having a molecular weight selected from the group consisting of about 15,000, about 21,000, about 23,000 and about 24,000, as determined by SDS-PAGE under reducing conditions.
- 5. The protein of claim 1, which is further characterized by having an amino acid sequence selected from the group consisting of SEQ ID NO:29, SEQ ID NO:31, SEQ ID NO:33 and SEQ ID NO:23.
- 6. The protein of claim 1, which is further characterized by having the amino acid sequence described in SEQ ID NO:23.
- 7. A composition comprising a therapeutically effective amount of augmenter of liver regeneration of claim 1.
- 8. A therapeutic agent for hepatocirrhosis comprising the protein of claim 1.
- 9. An isolated and purified protein which is a human augmenter of liver regeneration, said protein comprising an amino acid sequence selected from the group consisting of SEQ ID NO:23, SEQ ID NO:29, SEQ ID NO:31 and SEQ ID NO:33.
- 10. An isolated and purified protein which is a human augmenter of liver regeneration produced by the process of
- a) cultivating a transformant harboring an expression vector containing an exogenous DNA sequence capable of expressing said augmenter of liver regeneration and
- b) harvesting said augmenter of liver regeneration from culture;
- wherein said human augmenter of liver regeneration has an amino acid sequence selected from the group consisting of SEQ ID NO:29, SEQ ID NO:31, SEQ ID NO:33 and SEQ ID NO:23.
- 11. An isolated and purified protein which is a human augmenter of liver regeneration produced by the process of
- a) cultivating a transformant harboring an expression vector containing an exogenous DNA sequence capable of expressing said augmenter of liver regeneration and
- b) harvesting said augmenter of liver regeneration from culture;
- wherein said DNA sequence is selected from the group consisting of SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:32 and SEQ ID NO:22.
Parent Case Info
This is a division of application Ser. No. 08/367,968, filed Jan. 3, 1995, now U.S. Pat. No. 5,607,844, which is a Continuation-In-Part of U.S. patent application Ser. No. 08/197,496, filed Feb. 16, 1994, now U.S. Pat. No. 5,480,797, and a Continuation-In-Part of U.S. patent application Ser. No. 08/275,370, filed Jul. 15, 1994, now U.S. Pat. No. 5,550,037, the entire contents of each are hereby incorporated by reference and relied upon.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
5440022 |
Wu et al. |
Aug 1995 |
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Non-Patent Literature Citations (9)
Entry |
Yao et al., Hepatology 12:1144-1151, 1990. |
Labrecque, Dig. Dis. Sci. 36:669-673, 1991. |
Francavilla et al., Dig. Dis. Sci. 36:674-680, 1991. |
Yao et al., Chin. Med. J. 106:527-532 (1993). |
Zhou et al., Chin. Med. Sci. J. 7:197-200 (1992). |
He et al., "Human Hepatic Stimulator Substance: A Product of Gene Expression of Human Fetal Liver Tissue," Hepatology, Feb. 1993, vol. 17, pp. 225-229. |
Francavilla et al., "Extraction and Partial Purification of a Hepatic Stimulatory Substance in Rats, Mice, and Dogs," Cancer Research 47, 5600-5606, Nov. 1, 1987. |
LaBrecque et al., "Purification and Physical-Chemical Characterization of Hepatic Stimulator Substance," Hepatology, vol. 7, No. 1, 1987, pp. 100-106. |
Hagiya et al., "Cloning and sequence analysis of the rat augmenter of liver regeneration (ALR) gene: Expression of biologically active recombinant ALR and demonstration of tissue distribution," Proc. Natl. Acad. Sci. USA, vol. 91, pp. 8142-8146, Aug. 1994 pp. 8142-8146. |
Related Publications (1)
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Number |
Date |
Country |
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275370 |
Jul 1994 |
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Divisions (1)
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Number |
Date |
Country |
Parent |
367968 |
Jan 1995 |
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Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
197496 |
Feb 1994 |
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