Claims
- 1. An isolated or non-human host cell transfected with a first expression vector comprising a first nucleic acid encoding a first polypeptide of SEQ ID NO: 2 and a second expression vector comprising a second nucleic add encoding a second polypeptide of SEQ ID NO: 12.
- 2. The host cell of claim 1, wherein the host cell is:a) a prokaryotic cell; b) a mammalian cell; c) an Insect cell; or d) a yeast cell.
- 3. A method of producing a soluble polypeptide complex of SEQ ID NO:2 and SEQ ID NO:12 comprising:a) culturing the host cell of claim 1 under conditions suitable for expression of the first and second polypeptide; b) allowing the polypeptide complex to form; and c) isolating or purifying the soluble polypeptide complex.
- 4. An expression vector comprising:a) a first nucleic acid encoding a first polypeptide of SEQ ID NO: 2 operably linked to a first promoter; and b) a second nucleic acid encoding a second polypeptide of SEQ ID NO: 12 operably linked to a second promoter.
- 5. An isolated or non-human host cell comprising the expression vector of claim 4.
- 6. The host cell of claim 5, wherein the host cell is:a) a prokaryotic cell; b) a mammalian cell; c) an insect cell; or d) a yeast cell.
- 7. A method of producing a soluble polypeptide complex of SEQ ID NO:2 and SEQ ID NO:12 comprising:a) culturing the host cell of claim 5 under conditions suitable for expression of the first polypeptide and the second polypeptide; b) allowing the polypeptide complex to form; and c) isolating or purifying the soluble polypeptide complex.
Parent Case Info
This filing is a conversion to U.S. Utility Patent Application of U.S. Ser. No. 60/124,319, filed Mar. 11, 1999, which is incorporated herein by reference.
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Provisional Applications (1)
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Number |
Date |
Country |
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Mar 1999 |
US |