Claims
- 1. An isolated polynucleotide which encodes a mammalian Zcyto7 polypeptide wherein said polynucleotide encodes a polypeptide comprised of an amino acid sequence selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 12, SEQ ID NOs:14-25 and SEQ ID NOs: 39 and 40 or a polypeptide that is at least 90% identical to said polypeptides.
- 2. The polynucleotide of claim 1 wherein the polypeptide is fused to a carrier polypeptide or other carrier molecule.
- 3. An expression vector comprising the following operably linked elements:
a transcription promoter; a DNA segment which encodes a polypeptide comprised of an amino acid sequence selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 12, SEQ ID NOs: 14-25 and SEQ ID NOs: 39 and 40 or a polypeptide that is at least 90% identical to said polypeptides; and a transcription terminator.
- 4. An expression vector comprising the following operably linked elements:
(a) a transcription promoter; (b) a DNA segment encoding a chimeric polypeptide, wherein said chimeric polypeptide is comprised of a first portion and a second portion joined by a peptide bond, said first portion being comprised of a polypeptide comprised of an amino acid sequence selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 12, SEQ ID NOs:14-25 and SEQ ID NOs: 39 and 40 or a polypeptide that is at least 90% identical to said polypeptides and said second portion being a second polypeptide or protein. (c) a transcription terminator.
- 5. An isolated polypeptide comprised of an amino acid sequence selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 12, SEQ ID NOs: 14-25 and SEQ ID NOs: 39 and 40 or a polypeptide that is at least 90% identical to said polypeptides.
- 6. An antibody, antibody fragment or single-chain antibody that specifically binds to a mammalian polypeptide, said polypeptide being comprised of an amino acid sequence selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 12, SEQ ID NOs: 14-25 and SEQ ID NOs: 39 and 40 or a polypeptide that is at least 90% identical to said polypeptides.
- 7. An antibody of claim 6 wherein said antibody is either monoclonal or polyclonal.
- 8. The antibody, antibody fragment or single-chain antibody of claim 6 wherein said antibody, antibody fragment or single-chain antibody is humanized.
- 9. A method for producing an antibody which binds to a polypeptide comprised of an amino acid sequence selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 12, SEQ ID NOs: 14-25 and SEQ ID NOs: 39 and 40 or a polypeptide that is at least 90% identical to said polypeptides comprising inoculating an animal with said polypeptide or with a nucleic acid which encodes said peptide or polypeptide, wherein said animal produces antibodies to said or polypeptide; and isolating said antibody.
- 10. The method of claim 9 wherein said antibody is either a polyclonal or monoclonal antibody.
- 11. An anti-idiotypic antibody, anti-idiotypic antibody fragment or anti-idiotypic single-chain antibody which binds to an antibody, an antibody fragment or single-chain antibody that specifically binds to a polypeptide comprised of an amino acid sequence selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 12, SEQ ID NOs: 14-25 and SEQ ID NOs: 39 and 40 or a polypeptide that is at least 90% identical to said polypeptides.
Parent Case Info
[0001] This application is a continuation of U.S. application Ser. No. 09/066,745, filed Apr. 24, 1998, which claims the benefit of U.S. Provisional Application Serial No. 60/071,676 filed Jan. 16, 1998; and U.S. Provisional Application Serial No. 60/044,886 filed Apr. 25, 1997.
Provisional Applications (2)
|
Number |
Date |
Country |
|
60071676 |
Jan 1998 |
US |
|
60044886 |
Apr 1997 |
US |
Divisions (1)
|
Number |
Date |
Country |
Parent |
09611152 |
Jul 2000 |
US |
Child |
10340042 |
Jan 2003 |
US |
Continuations (1)
|
Number |
Date |
Country |
Parent |
09066745 |
Apr 1998 |
US |
Child |
09611152 |
Jul 2000 |
US |