The present invention relates to a mass spectrometric device and a mass spectrometric device control method.
As a device for quickly determining a component of trace material included in a sample, a small and lightweight mass spectrometric device (often referred to as MS) has become necessary. In particular, a market is expanding as a sensing device of an illegal drug and an explosive. The mass spectrometric device ionizes a molecule in the sample to be analyzed, and detects an ion ionized by mass separation using an electric field and a magnetic field with a detector.
As a method for ionizing the molecule in the sample, APCI (Atmospheric Pressure Chemical Ionization Source), an electron impact ionization method, glow discharge, and the like have been put into practice; however, there are many inadequate points such as low ionization efficiency, and occurrence of fragmentation. Therefore, high precision adjustment is required to cope with these inadequate points, and the device tends to become large. On the other hand, as a relatively new method that is superior in terms of the ionization efficiency and the fragmentation, an atmospheric pressure dielectric barrier discharge method has begun to be studied in recent years. The barrier discharge ionizes the molecule in the sample by making a discharge current flow by applying a pulse-like or sine-wave-like high voltage via a dielectric barrier to a discharge unit in which the sample is introduced and that has a pressure close to the atmospheric pressure.
As a mass spectrometric device using the barrier discharge in an ionization unit, there are techniques described in PATENT LITERATURE 1 (JP 2012-104247 A), PATENT LITERATURE 2 (PCT/US2008/065245), PATENT LITERATURE 3 (PCT/JP2009/060653).
In PATENT LITERATURE 1, there is provided a small and lightweight mass spectrometric device capable of high precision mass spectrometry. The mass spectrometric device has an ionization source for ionizing a gas flowing in from the outside for ionizing a measurement sample, and a mass spectrometry unit for separating the ionized measurement sample. The barrier discharge is used for the ionization source. In PATENT LITERATURE 1, the mass spectrometric device has a suppression means for suppressing a flow rate of the gas taken into the ionization source, and an opening/closing means for opening and closing flow of the gas taken into the ionization source. By making the gas introduced from the outside intermittently flow into the ionization unit, and also by operating the barrier discharge unit intermittently at a pressure of 100 Pa to 10000 Pa lower than the atmospheric pressure, high efficiency and downsizing are achieved.
In PATENT LITERATURE 2, it is described about a method for obtaining high efficiency by ionizing the sample at the atmospheric pressure with the barrier discharge in the mass spectrometric device and discontinuously introducing the ionized sample to the mass spectrometry unit.
In PATENT LITERATURE 3, it is described about a method for improving ionization efficiency of the sample by devising electrode structure of the barrier discharge unit.
Although it is not an example of the barrier discharge, as a stabilization technique of the discharge unit, in relation to a device for detecting the discharge current, there are devices disclosed in PATENT LITERATURE 4 (JP 2011-232071 A), PATENT LITERATURE 5 (JP 2008-53020 A).
In PATENT LITERATURE 4, the device performs high S/N ionization current detection by detecting the discharge current of the discharge unit and integrating the ionization current in the device only in a period in which the discharge current flows.
In PATENT LITERATURE 5, it is described about a method for achieving noise reduction by detecting a current flowing through a discharge electrode and controlling an applied voltage so that the current becomes a predetermined current, to stabilize the ionization with the APCI (Atmospheric Pressure Chemical Ionization method) and reduce a noise level in the mass spectrometric device.
Patent Literature 1: JP-A-2012-104247
Patent Literature 2: PCT/US2008/065245
Patent Literature 3: PCT/JP2009/060653
Patent Literature 4: JP-A-2011-232071
Patent Literature 5: JP-A-2008-53020
It has become apparent by experiments that, in the barrier discharge, depending on the surrounding environment, variation occurs in an applied high voltage at the time of discharge-start, and in time from the high voltage application start to the discharge start.
In documents in CITATION LIST, the ionization efficiency is improved by such as pressure reduction of the ionization unit, intermittent operation of the ionization source, electrode structure optimization of the ionization source, and the discharge current is detected and the applied voltage is controlled so that the discharge current becomes the predetermined discharge current, and the S/N of the measurement value is improved by measuring the ionization current only in the period in which the discharge current flows, however, variation in the discharge-start voltage and variation in discharge-start time are not focused.
In addition, in a mass spectrometric device that intermittently operates the ionization source, an atmosphere is ionized by causing the barrier discharge by applying a high voltage to the atmosphere of a low pressure multiple times intermittently, and a measured object is ionized by the ionized body to perform mass spectrometry. Since each application period is constant of the high voltage to be applied multiple times, when variation occurs in the time from the high voltage application start to the discharge start as described above, there are problems that variation occurs in a period in which the barrier discharge is caused depending on each period, change occurs in an amount of the measured object to be ionized, and accuracy of a mass spectrometry result is degraded.
Therefore, the present invention aims to provide a mass spectrometric device and a mass spectrometric device control method that suppress change of the amount of the measured object to be ionized and accuracy degradation of the mass spectrometry result.
To solve the above problems, a configuration is adopted according to the appended claims, for example.
The present application includes several means for solving the above problems, and an example thereof is a mass spectrometric device including: a sample container for containing a measurement sample; a detector for detecting a drug and the like included in the sample by analyzing mass of the sample; a dielectric container for coupling with the sample container and for ionizing an atmosphere by making a discharge current flow through the atmosphere; a valve for intermittently sending the atmosphere to the sample container, the dielectric container, and the detector; a barrier discharge high-voltage power source for causing discharge in the dielectric container; a current detection unit connecting with the barrier discharge high-voltage power source and for detecting a discharge current; a discharge-start timing detection unit for connecting with the current detection unit and for detecting discharge-start timing based on a current detection result of the current detection unit to transmit a discharge-start timing signal; and a control unit for controlling each constituent, wherein the current detection unit converts the detected current to a voltage, and compares the converted voltage with a threshold set in the discharge-start timing detection unit, and transmits a discharge-start signal to the control unit when the converted voltage exceeds the threshold, and the control unit performs control to cause discharge for a certain period after receiving the discharge-start signal.
In the present invention, a mass spectrometric device and a mass spectrometric device control method can be provided that suppress change of an amount of a measured object to be ionized and accuracy degradation of a mass spectrometry result.
In the following, embodiments are described with reference to the drawings.
In the present embodiment, a configuration and a control method are presented for detecting discharge-start timing by using discharge current detection and for controlling a high voltage output by using the timing.
At sequence 1 (S1), the mass spectrometry is started. At sequence 2 (S2), the valve 2 is closed. At sequence 3 (S3), gases in the dielectric container 3 and the detector 9 are exhausted by the vacuum pump 14 to reduce the pressures (for example, 100 Pa in the dielectric container 3, 0.1 Pa in the detector 9). At sequence 4 (S4), by opening the valve 2, the atmosphere is introduced to the dielectric container 3 via the capillary 1.
After introducing the atmosphere, a predetermined time has elapsed and an inside of the dielectric container 3 is filled with the atmosphere of a low pressure (for example, 1000 Pa), and then at sequence 5 (S5), by applying a pulse-like high voltage to the electrode 4, electrode 4′ from the barrier discharge high-voltage power source 6 and causing the barrier discharge in the dielectric container, the introduced atmosphere of the low pressure is ionized (reactant ion generation).
After completion of the barrier discharge, at sequence 6 (S6), the valve 2 is closed. The atmosphere including the reactant ion is introduced to the sample container 8 to ionize a sample 12 of the inside. At sequence 7 (S7), the ionized sample 12 is introduced to the detector 9 to be trapped and accumulated in the detector 9. At the same time, exhaust is started by the vacuum pump 14, and an unnecessary atmosphere is exhausted, and the pressures in the dielectric container 3 and the detector 9 are reduced again.
Then, at sequence 8 (S8), the ionized state sample 12 trapped and accumulated in the detector 9 is processed in the detector 9 to detect the drug and the like included in the sample 12. When the mass detection operation is continued, the operation is returned to sequence 4 (S4), and the sequences described above are repeated, and after completing n times of repetition that is the number of times of repetition determined in the control circuit 11, at sequence 9 (S9), the mass spectrometry is completed.
Incidentally, for the mass spectrometry result, an average of results in the n times of repetition can be used as a detection result, and the most sensitive result can be used as a detection result, and only some measurement results of the n times of repetition can be used as detection results.
Described above is the general flow of the mass spectrometry. Here, it is described for the detailed sequence according to the present embodiment. At sequence 5 (S5), the pulse-like high voltage is applied to the electrode 4, electrode 4′ from the barrier discharge high-voltage power source 6. In a period in which the barrier discharge is caused in the dielectric container, at sequence 51 (S51), the current detection unit 5 detects the discharge current 28 that flows due to the high voltage applied to the electrode from the barrier discharge high-voltage power source 6. From the detection result at sequence 52 (S52), the discharge-start timing detection unit 7 detects the timing at which the discharge is caused in the period in which the high voltage is applied. At sequence 53 (S53), in the control circuit 11, for a certain period from the discharge-start timing, by controlling the barrier discharge high-voltage power source 6 to output the high voltage to apply to the electrode 4, the discharge period is controlled to be constant.
As described above, in the repeated mass detection operation from sequence 4 (S4) to sequence 8 (S8), the barrier discharge period at sequence 5 (S5) is controlled to be a constant period, so that an amount of a measured object to be ionized becomes constant at any operation of the n times of repeated operation, and there is an effect of improving accuracy of the mass spectrometry result.
The coil 22 is terminated by an integral resistance 21. A discharge detection current 24, which is induced in the coil 22 by the discharge current 28 flowing through the high-voltage cable 19, is converted to a voltage. The converted voltage is input to the discharge-start timing detection unit 7 to detect the discharge-start timing.
In the present configuration, when the discharge is caused, an induction current is induced in the coil 22 due to the discharge current 28 flowing through the high-voltage cable 19. The induction current is converted to an induction voltage by the integral resistance. When the induction voltage exceeds a predetermined threshold, the discharge-start timing detection unit 7 determines that the discharge is started, and a timing pulse is output to the counter 15 of the control circuit 11. With the present configuration, since the discharge current is detected using the induction current induced in the coil, a noise-resistant, stable discharge current detection is possible.
Since the period in which the high voltage 23 is applied is the same period in each of the sequences, as a result, the discharge periods become different periods τ1, τ2, τ3, τ4.
On the other hand, discharge timing chart (b) is a timing chart in a configuration of the present invention that detects the discharge-start timing. This is an example of the mass spectrometry flow in
In the present embodiment, a configuration and a control method are presented for estimating the discharge-start timing by using pressure detection results in the dielectric container 3 and the detector 9 and for controlling the high voltage output by using the timing.
At sequence 5 (S5), the pulse-like high voltage is applied to the electrode 4, electrode 4′ from the barrier discharge high-voltage power source 6. In a period in which the barrier discharge is caused in the dielectric container, at sequence 501 (S501), the pressure detector 10 detects the pressures in the detector 9 and the dielectric container 3. At sequence 502 (S502), from a pressure detection result of the pressure detector 10, the timing is estimated at which the discharge is caused in the period in which the high voltage is applied. As a method for estimating the discharge timing, when a pressure detection value of the pressure detector 10 exceeds a pressure reference value preset in the control circuit 11, it is determined that the discharge is started, and that point of time is made to be the discharge-start timing.
At sequence 503 (S503), based on the estimation result, in the control circuit 11, for a certain time from the estimation discharge-start timing, the discharge period is controlled to be constant by outputting a high voltage from the barrier discharge high-voltage power source 6 to apply to the electrode 4.
As described above, in the repeated mass detection operation from sequence 4 (S4) to sequence 8 (S8), the barrier discharge period at sequence 5 (S5) is controlled to be a constant period, so that an amount of a measured object to be ionized becomes constant at any operation of the n times of repeated operation, and there is an effect of improving accuracy of the mass spectrometry result.
In the present embodiment, a configuration and a control method are presented for detecting whether or not the discharge current flows by using discharge current detection and for controlling a high voltage output when the discharge current does not flow.
First,
At sequence 5 (S5), the pulse-like high voltage is applied to an electrode 4, electrode 4′ from the barrier discharge high-voltage power source 6. In a period in which the barrier discharge is caused in a dielectric container 3, at sequence 100 (S100), a current detection unit 5 detects a discharge current 28 that flows due to the high voltage applied to the electrode from the barrier discharge high-voltage power source 6. From the detection result, a discharge-start timing detection unit 7 detects the timing at which the discharge is caused in the period in which the high voltage is applied.
At this time, when the discharge-start timing detection unit 7 does not detect the discharge, at sequence 101 (S101), a discharge voltage detection signal 28 is fed back to a control circuit 11 to increase the discharge voltage. When the discharge-start timing detection unit 7 detects the discharge, the discharge voltage detection signal 28 is fed back to the control circuit 11 not to change the discharge voltage.
As described above, in the repeated mass detection operation from sequence 4 (S4) to sequence 8 (S8), it is detected whether or not the discharge current flows, and when the discharge current does not flow, the applied high voltage is controlled to be increased in the next flow, so that an amount of a measured object to be ionized is stabilized in some of the n times of repeated operation, and there is an effect of improving accuracy of the mass spectrometry result.
On the other hand, discharge timing chart (b) is a timing chart in a configuration of the present invention according to the present embodiment that detects the discharge-start timing. In the mass spectrometry flow in
Number | Date | Country | Kind |
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2013-141300 | Jul 2013 | JP | national |
Filing Document | Filing Date | Country | Kind |
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PCT/JP2014/064359 | 5/30/2014 | WO | 00 |
Publishing Document | Publishing Date | Country | Kind |
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WO2015/001881 | 1/8/2015 | WO | A |
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Number | Date | Country |
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2008-053020 | Mar 2008 | JP |
2009-060653 | Mar 2009 | JP |
2011-232071 | Nov 2011 | JP |
2012-104247 | May 2012 | JP |
2013-037815 | Feb 2013 | JP |
2009023361 | Feb 2009 | WO |
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Number | Date | Country | |
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20160141163 A1 | May 2016 | US |