Patent Grant
7658927
Domestic cats are subject to infection by several retroviruses, including feline leukemia virus (FeLV), feline sarcoma virus (FeSV), endogenous type C oncoronavirus (RD-114), and feline syncytia-forming virus (FeSFV). Of these, FeLV is the most significant pathogen, causing diverse symptoms including lymphoreticular and myeloid neoplasms, anemias, immune-mediated disorders, and an immunodeficiency syndrome that is similar to human acquired immune deficiency syndrome (AIDS). Recently, a particular replication-defective FeLV mutant, designated FeLV-AIDS, has been more particularly associated with immunosuppressive properties.
The discovery of feline T-lymphotropic lentivirus (now designated as feline immunodeficiency virus, FIV) was first reported in Pedersen et al. (1987). Characteristics of FIV have been reported in Yamamoto et al. (1988a); Yamamoto et al. (1988b); and Ackley et al. (1990). Seroepidemiologic data have shown that infection by FIV is indigenous to domestic and wild felines throughout the world. A wide variety of symptoms are associated with infection by FIV, including abortion, alopecia, anemia, conjunctivitis, chronic rhinitis, enteritis, gingivitis, hematochezia, neurologic abnormalities, periodontitis, and seborrheic dermatitis. The immunologic hallmark of domestic cats infected with FIV is a chronic and progressive depletion of feline CD4+ peripheral blood lymphocytes, a reduction in the CD4:CD8 cell ratio and, in some cases, an increase in CD8-bearing lymphocytes. Based on molecular, biochemical and immunopathologic characteristics, FIV infection of cats is now considered to be a better feline AIDS model than FeLV-FAIDS.
Cloning and sequence analysis of FIV has been reported in Olmsted et al. (1989a); Olmsted et al. (1989b); and Talbott et al. (1989). Hosie and Jarrett (1990) described the serological response of cats infected with FIV. FIV virus subtypes can be classified according to immunotype based on the level of cross-neutralizing antibodies elicited by each strain (Murphy and Kingsbury, 1990). Recently, viruses have been classified into subtypes according to genotype based on nucleotide sequence homology. Although HIV and FIV subtyping is based on genotype (Sodora et al., 1994; Rigby et al., 1993; and Louwagie et al., 1993), little is known about the correlation between the genotype and immunotype of subtypes. FIV viral isolates have been classified into four FIV subtypes: A, B, C and D. (Kakinuma et al., 1995). Infectious isolates and infectious molecular clones have been described for all FIV subtypes except for subtype C (Sodora et al., 1994). Subtype C FIV has originally been identified from cellular DNA of cats from Canada (Sodora et al., 1994; Rigby et al., 1993; Kakinuma et al., 1995). FIV strains identified in the art include (subtype of the strain is shown in parenthesis) Petaluma (A), Dixon (A), UK8 (A), Dutch113 (A), Dutch19K (A), UK2 (A), SwissZ2 (A), Sendai-1 (A), USCAzepy01A (A), USCAhnky11A (A), USCAtt-10A (A), USCAlemy01 (A), USCAsam-01A (A), PPR (A), FranceWo, Netherlands, Bangston (A/B), Aomori-1 (B), Aomori-2 (B), USILbrny03B (B), TM2 (B), Sendai-2 (B), USCKlgri02B (B), Yokohama (B), USMAsboy03B (B), USTXmtex03B (B), USMCglwd03B (B), CABCpbar03C (C), CABCpbar07C (C), CABCpady02C (C), Shizuoka (D), and Fukuoka (D).
Although major strides have been made with antiviral drug therapy, the development of an effective vaccine against HIV-1 is still considered central to the control of the AIDS epidemic (Calarota et al., 2003). Multiple HIV vaccine designs using different HIV-1 strains are currently in various phases of clinical trials (Calarota et al., 2003; Cohen, 2003; IAVI Report. Ongoing trials of preventive HIV vaccines (last updated: Dec. 14, 2004). IAVI Report Online Special Features: www.iavireport.org/specials/OngoingTrialsofPreventiveHIVVaccines.pdf). Even in light of these clinical trials, it is still unclear what HIV-1 epitopes and immune mechanisms are essential for vaccine protection (Calarota et al., 2003; McMichael et al., 2003). Similar issues were faced during the development of an FIV vaccine for domestic cats (Uhl et al., 2002). As a means to broaden FIV vaccine efficacy, a dual-subtype vaccine was developed using FIV strains from long-term nonprogressor cats (Uhl et al., 2002; Yamamoto et al., 2002). This vaccine demonstrated moderate to significant protection of cats against both homologous and heterologous FIV challenges (Uhl et al., 2002; Yamamoto et al., 2002, Pu et al., 2001; Pu et al., 2005). Furthermore, this vaccine induced not only broad neutralizing antibodies (Pu et al., 2001) but antibodies cross-reactive to HIV-1 proteins, especially to HIV-1 core protein (p24) and group-specific antigens (Gag) (Pu et al., 2002). The FIV epitopes responsible for providing the dual-subtype vaccine protection have yet to be determined.
Gag and other antigens conserved among viruses from the same subfamily frequently induce antibodies that cross-react with other subfamily members (Pu et al., 2002; Matsuo et al., 1992; Nath et al., 2001; Zvelebil et al., 1988; Murphy, 1996). Some cross-reactive antigens have been used as immunogens for vaccine against viruses from the same subfamily (Henderson et al., 2004; Yazbak et al., 2002). Classic examples of such vaccines are the use of vaccinia virus vaccines for smallpox in humans and human measles vaccines for canine distemper in puppies (Henderson et al., 2004; Yazbak et al., 2002). Consequently, protective vaccines based on cross-reactive antigens have been shown to provide broad immunity, and may be useful against viruses that are currently evolving in a new host, such as HIV infection of humans.
Although cross-protection against HIV-1 with prior HIV-2 infection has been reported in multiple retrospective studies (Travers et al., 1995; Greenberg et al., 1996), controversy still exists with multiple studies reporting no protection (Norrgren et al., 1999; Schim van der Loeff et al., 2001). Even though amino acid (aa) sequences of the structural gene products exhibit only limited identity (<60%) between HIV-1 and HIV-2 (Guyader et al., 1987), some of the cross-reactive epitopes between these two major HIV groups have been reported to induce cross-neutralizing antibodies and cross-reactive cytotoxic T lymphocyte (CTL) activities (Robert-Guroff et al., 1992; Bottiger et al., 1990; Nixon et al., 1990; Rowland-Jones et al., 1995). Moreover, poxvirus-vectored recombinant HIV-1 vaccine priming followed by HIV-1 protein boost, conferred cross-protection against HIV-2 challenge in macaques (Abimiku et al., 1995). However, cross-reactive antigen-induced protective immunity has not been reported against distinct heterologous-species viruses (HIV-1 and FIV).
The subject invention pertains to methods and compositions for protecting feline animals from infection by FIV using immunogens derived from primate immunodeficiency viruses, including HIV and SIV. Methods and compositions for vaccinating feline animals with the subject vaccine compositions are described. Feline animals vaccinated according to the methods and compositions of the subject invention exhibit protective immune responses to FIV when challenged with FIV.
The subject invention also concerns methods for selecting for epitopes that are evolutionarily conserved among immunodeficiency viruses. Evolutionarily conserved epitopes of immunodeficiency viruses identified using methods of the present invention also constitute part of the invention. The subject invention further concerns methods and compositions for protecting humans and other animals against infection by immunodeficiency viruses, such as HIV and FIV.
The file of this patent contains at least one drawing executed in color. Copies of this patent with the color drawing will be provided by the Patent and Trademark Office upon request and payment of the necessary fee.
The FIV infection results from PBMC based on RT activity (RT) and/or proviral PCR (PCR) are shown with either positive (+) or negative (−) immediately below the corresponding time points. The RT/PCR results for LN and BM cells are shown on the row designated as LN/BM with the first result (+/ or −/) for LN cells and the second result (/+ or /−) for BM cells. All vaccinated/unprotected cats (#IZ1, [two cats not shown], Study 3; #AA1, Study 4) and all adjuvant-immunized and PBS-immunized control cats (#JB2, #IW2, #MK2, [three cats not shown], Study 3; #MD3, #MG4, #MK4, Study 4) after challenge were positive FIV infection by 3-12 wpi in virus isolation/PCR analyses and by 3-15 wpi in immunoblot analysis (immunoblots of 15 wpi not shown). The earliest time points of virus detection in challenged control cats were 3 wpi for Study 3 (FIVBang) and 6 wpi for Study 4 (FIVFC1), which were identical to the earliest time points of FIV antibody detection in these cats by immunoblot analysis. Based on the infected-control-cat results, none of the vaccinated/unprotected cats in Studies 3 (#IZ1, two other cats not shown) and 4 (#AA1) had enhancement in FIV infection.
Sequence homology analysis between HIV-1UCD1 and FIVBang is shown with (.) for aa homology and (:) for aa identity according to the GeneStream Align program (GeneStream Align program. http://xylian.igh.cnrs.fr/) (
FeIFNγ ELISpot analysis was used to determine the T-cell epitope reactivity of PBMC from vaccinated/protected cats and infected control cats (panels B1, B2, B3 of
SEQ ID. NO: 1 is an amino acid sequence of an HIV-1UCD1 envelope protein.
SEQ ID NO: 2 is an amino acid sequence of an HIV-1UCD1 gag protein.
SEQ ID NO: 3 is a nucleotide sequence of an HIV-1UCD1 polynucleotide encoding an envelope protein.
SEQ ID NO: 4 is a nucleotide sequence of an HIV-1UCD1 polynucleotide encoding a gag protein.
SEQ ID. NO: 5 is an amino acid sequence of an HIV-1IIIB envelope protein.
SEQ ID NO: 6 is an amino acid sequence of an HIV-1IIIB gag protein.
SEQ ID NO: 7 is a nucleotide sequence of an HIV-1IIIB polynucleotide encoding an envelope protein.
SEQ ID NO: 8 is a nucleotide sequence of an HIV-1IIIB polynucleotide encoding a gag protein.
SEQ ID NO: 9 is an amino acid sequence of a peptide fragment (H2) of an HIV protein that can be used according to the subject invention.
SEQ ID NO: 10 is an amino acid sequence of a peptide fragment (H3) of an HIV protein that can be used according to the subject invention.
SEQ ID NO: 11 is an amino acid sequence of a peptide fragment (H4) of an HIV protein that can be used according to the subject invention.
SEQ ID NO: 12 is an amino acid sequence of a peptide fragment (H4.5) of an HIV protein that can be used according to the subject invention.
SEQ ID NO: 13 is an amino acid sequence of a peptide fragment (H5) of an HIV protein that can be used according to the subject invention.
SEQ ID NO: 14 is an amino acid sequence of a peptide fragment (H6) of an HIV protein that can be used according to the subject invention.
SEQ ID NO: 15 is an amino acid sequence of a peptide fragment (H7) of an HIV protein that can be used according to the subject invention.
SEQ ID NO: 16 is an amino acid sequence of a peptide fragment (H7.8) of an HIV protein that can be used according to the subject invention.
SEQ ID NO: 17 is an amino acid sequence of a peptide fragment (H8) of an HIV protein that can be used according to the subject invention.
SEQ ID NO: 18 is an amino acid sequence of a peptide fragment (F2) of an FIV protein that can be used according to the subject invention.
SEQ ID NO: 19 is an amino acid sequence of a peptide fragment (F3) of an FIV protein that can be used according to the subject invention.
SEQ ID NO: 20 is an amino acid sequence of a peptide fragment (F4) of an FIV protein that can be used according to the subject invention.
SEQ ID NO: 21 is an amino acid sequence of a peptide fragment (F4.5) of an FIV protein that can be used according to the subject invention.
SEQ ID NO: 22 is an amino acid sequence of a peptide fragment (F5) of an FIV protein that can be used according to the subject invention.
SEQ ID NO: 23 is an amino acid sequence of a peptide fragment (F6) of an FIV protein that can be used according to the subject invention.
SEQ ID NO: 24 is an amino acid sequence of a peptide fragment (F7) of an FIV protein that can be used according to the subject invention.
SEQ ID NO: 25 is an amino acid sequence of a peptide fragment (F7.8) of an FIV protein that can be used according to the subject invention.
SEQ ID NO: 26 is an amino acid sequence of a peptide fragment (F8) of an FIV protein that can be used according to the subject invention.
SEQ ID NO: 27 is an amino acid sequence of a peptide fragment (FB1) of an FIV protein that can be used according to the subject invention.
SEQ ID NO: 28 is an amino acid sequence of a peptide fragment (FB2) of an FIV protein that can be used according to the subject invention.
SEQ ID NO: 29 is an amino acid sequence of a peptide fragment (FC2) of an FIV protein that can be used according to the subject invention.
SEQ ID NO: 30 is an amino acid sequence of a peptide fragment (FB3) of an FIV protein that can be used according to the subject invention.
SEQ ID NO: 31 is an amino acid sequence of a peptide fragment (FB4) of an FIV protein that can be used according to the subject invention.
SEQ ID NO: 32 is an amino acid sequence of a peptide fragment (FB5) of an FIV protein that can be used according to the subject invention.
SEQ ID NO: 33 is an amino acid sequence of a peptide fragment (FB6) of an FIV protein that can be used according to the subject invention.
SEQ ID NO: 34 is an amino acid sequence of a peptide fragment (FB7) of an FIV protein that can be used according to the subject invention.
SEQ ID NO: 35 is an amino acid sequence of a peptide fragment (FB8) of an FIV protein that can be used according to the subject invention.
SEQ ID NO: 36 is an amino acid sequence of a peptide fragment (FB9) of an FIV protein that can be used according to the subject invention.
SEQ ID NO: 37 is an amino acid sequence of a peptide fragment (FB10) of an FIV protein that can be used according to the subject invention.
SEQ ID NO: 38 is an amino acid sequence of a peptide fragment (FB11) of an FIV protein that can be used according to the subject invention.
SEQ ID NO: 39 is an amino acid sequence of a peptide fragment (HIV MHR) of an HIV protein that can be used according to the subject invention.
SEQ ID NO: 40 is an amino acid sequence of a peptide fragment (FIV MHR) of an FIV protein that can be used according to the subject invention.
SEQ ID NO: 41 is an amino acid sequence of a peptide fragment (F3.I) of an FIV protein that can be used according to the subject invention.
SEQ ID NO: 42 is an amino acid sequence of a peptide fragment (F4.5.I) of an FIV protein that can be used according to the subject invention.
SEQ ID NO: 43 is an amino acid sequence of a peptide fragment (F4.5.B) of an FIV protein that can be used according to the subject invention.
SEQ ID NO: 44 is an amino acid sequence of a peptide fragment (F5.B) of an FIV protein that can be used according to the subject invention.
SEQ ID NO: 45 is an amino acid sequence of a peptide fragment (F5.I) of an FIV protein that can be used according to the subject invention.
SEQ ID NO: 46 is an amino acid sequence of a peptide fragment (F7.B) of an FIV protein that can be used according to the subject invention.
The subject invention concerns materials and methods for protecting a feline animal from infection by FIV by administering to the animal immunogens derived from primate immunodeficiency viruses, such as human immunodeficiency virus (HIV) or simian immunodeficiency virus (SIV). In one embodiment, an effective amount of a composition comprising an immunogen or immunogens derived from a primate immunodeficiency virus is administered to a feline animal. In addition, the various immunogen compositions described herein can be used separately and in combination with each other.
Advantageously, the present invention allows for protection against FIV infection wherein the generation of an immune response against FIV in the animal allows a veterinarian or other person of ordinary skill in the art to determine whether an animal's immune response to FIV or FIV immunogens or antigens is a result of an immunization to protect against FIV infection or if it is a result of FIV infection in the animal. In one embodiment, animals immunized using a composition of the present invention do not produce antibodies that cross-react with FIV gp95. Animals that have been infected with FIV or that may have received vaccines containing FIV-derived immunogens or antigens produce antibodies that bind to FIV gp95. Feline animals encompassed within the scope of the present invention include domestic house cats, feral cats, and other wild cats including bobcat, cougar, mountain lion, tiger, jaguar, leopard, puma, cheetah, and lion.
The present invention can also be used to generate an immune response, cellular and/or humoral, against FIV in a feline animal using immunogens derived from primate immunodeficiency viruses, such as HIV and SIV. In one embodiment, an amount of an immunogen sufficient to induce an immune response against FIV is administered to a feline animal. Serum antibodies from immunized cats can be tested for virus neutralizing antibody activity against HIV using PBMCs from healthy HIV-uninfected humans as indicator cells. Lymphocytes from immunized cats can be tested for both HIV-specific T-helper (Th) and cytotoxic T lymphocyte (CTL) activities. Th and CTL activity can be measured by the level of Th cytokines and CTL cytotoxins produced by the lymphocytes from unimmunized and immunized cats in response to in vitro stimulation with inactivated HIV whole-virus that are either the same as (homologous) or different from (heterologous to) the immunogen strain(s). The inactivated heterologous strains used for the in vitro stimulation can be from the same or different subtypes as the immunogen strain(s). Preferred immunogens produce high levels of Th cytokines in the cultures with CD4+ T lymphocytes from immunized cats and/or induce strong Th activity against FIV and/or HIV. Similarly, preferred immunogens produce high levels of CTL cytotoxin(s) by CD8+ T lymphocytes from immunized cats and/or induce strong CTL activity against FIV and/or HIV.
Primate immunodeficiency viruses encompassed within the scope of the methods and materials of the present invention include HIV and SIV. In regard to HIV, the virus can be from either HIV-1 or HIV-2. In one embodiment, the HIV is HIV-1. Several distinct strains of HIV-1 have been described in the art and include, for example, HIV-1IIIB, HIV-1UCD1, HIV-1LAI/LAV, and HIV-1BRU. In an exemplified embodiment described herein, immunogens of the present invention are derived from the strain of HIV-1 designated as HIV-1IIIB and/or HIV-1UCD1. SIV strains within the scope of the invention include, but are not limited to, SIVsyk, SIVsmm, SIVmac, SIVmnd, SIV1′hoest, SIVagm and SIVcpz (Whetter et al., 1999). The nucleotide sequences of numerous HIV and SIV genes have been described in the scientific literature, deposited in sequence databanks, such as the NCBI Genbank, and have been disclosed in published patent applications and issued patents. For example, complete genome sequences of HIV-1 and HIV-2 isolates are disclosed at Genbank accession numbers NC 001802 and NC 001722 and complete genome sequences of SIV are disclosed at Genbank accession numbers L06042, AF131870, M32741, and M66437. Similarly, amino acid sequences of proteins encoded by HIV and SIV genes have been disclosed and are well known in the art. Sequences of HIV are also disclosed in databases available at the website http://www.hiv.lanl.gov/content/index. Genes of primate immunodeficiency viruses that encode proteins that can be used in the present invention, but are not limited to, include env, gag, pol, and nef and fragments and subunits thereof.
Immunogens derived from primate immunodeficiency viruses can be in isolated form or provided in any suitable composition. Preferably, the primate immunodeficiency viral immunogens are provided in a composition and administered in a manner to induce a strong cell-mediated and humoral immune response to the immunogen in a feline animal. In one embodiment, immunogens can be provided in the form of virus infected cells or whole cell-free virus. Virus in virus-infected cells and cell-free virus can be treated in a manner to inactivate or attenuate the virus. Methods for inactivating or attenuating virus are known in the art, and include, for example, treatment with paraformaldehyde, formalin, phenol, UV light, elevated temperature and the like. U.S. Pat. No. 6,503,753 describes methods for photoinactivation of HIV reverse transcriptase which thereby inactivates the HIV virus. Primate immunodeficiency viruses can also be prepared that are replication-defective. U.S. Pat. No. 6,500,623 describes HIV replication-defective viral particles and means for producing them. Other techniques for producing inactivated, attenuated and replication defective viruses are known in the art.
In another embodiment, immunogens of the invention are derived from viral proteins, or immunogenic fragments or variants thereof, of primate immunodeficiency viruses. Proteins that can be utilized as immunogens in the present invention include, but are not limited to, proteins encoded by the env, gag, pol, tat, rev, and/or nef genes. In one embodiment, envelope proteins encoded by the env gene that can be used in the invention include the envelope proteins gp120 and gp160, including fragments thereof, fusions of gp120 and gp160, and variations of gp120 and gp160, including variations in glycosylation which includes gp120 and/or gp160 proteins that are unglycosylated. A cellular protein cleaves gp160 into gp41 and gp120 moieties. The gp41 moiety comprises the transmembrane domain of the envelope protein, whereas gp120 is located on the surface of the infected cell or virion. The gp120 molecule consists of a polypeptide core of approximately 60 kd, but N-linked glycosylation can increase the molecular weight of the protein to approximately 120 kd. The viral envelope proteins used in conjunction with the present invention can be either unclipped or clipped (see, for example, U.S. Pat. No. 5,849,533). Envelope protein variants have been described in U.S. Pat. No. 5,846,546. Synthetic peptides which immunologically mimic HIV gp120 and/or gp160 envelope proteins can also be used in the present invention (see, for example, U.S. Pat. No. 5,763,160). In an exemplified embodiment, a combination of gp120 and gp160 envelope protein from HIV-1IIIB is utilized as an immunogen. In one embodiment, the envelope polypeptide of HIV-1UCD-1 comprises the amino acid sequence shown in SEQ ID NO: 1. In one embodiment, the envelope polypeptide of HIV-1IIIB comprises the amino acid sequence shown in SEQ ID NO: 5.
Proteins encoded by a primate immunodeficiency virus gag gene, such as the p17 matrix protein, the p24 capsid protein, and the p7 nucleocapsid protein of HIV and the p27 protein of SIV, and fragments and variants thereof, can also be used in accordance with the present invention. In an exemplified embodiment, a p24 protein from HIV-1UCD-1 is utilized as an immunogen. In one embodiment, the p24 polypeptide of HIV-1UCD-1 comprises amino acids 132 to 362 of SEQ ID NO: 2. In one embodiment, the p24 polypeptide of HIV-1IIIB comprises amino acids 132 to 363 of SEQ ID NO: 6. Immunological fragments of the p24 protein have been described in U.S. Pat. No. 5,700,469.
Proteins encoded by a primate immunodeficiency virus pol gene, such as reverse transcriptase (RT), protease (PR), and Rnase H of HIV, and fragments and variants thereof, can also be used in the present invention. Endonuclease protein p31 of HIV, and fragments and variants thereof, which is a protein encoded at the 3′-end of the pol gene of HIV, can also be used in accordance with the present invention. Immunological peptide fragments of the p31 protein of HIV have been described in U.S. Pat. No. 5,401,628. Regulatory proteins encoded by tat and rev genes of primate immunodeficiency viruses, and accessory proteins encoded by nef, vif, vpr, vpu, and vpx genes of primate immunodeficiency viruses, and fragments and variants of these proteins, can also be used in the present invention.
Peptides corresponding to immunogenic or antigenic regions of primate immunodeficiency viruses can be synthesized or prepared by recombinant means and utilized in the methods of the present invention. In one embodiment, peptides that correspond to overlapping fragments of a primate immunodeficiency virus protein can be prepared and used in the methods of the present invention. Any suitable combination of the peptide fragments can be used for immunizing an animal according to methods of the present invention. For example, peptide fragments that correspond to the carboxy terminus region of a viral protein can be used with peptide fragments that correspond to an amino terminus region or a transmembrane region of the protein.
Recombinant virus or viral vector-based expression constructs that comprise, primate immunodeficiency virus nucleotide sequences, for example, HIV or SIV env, gag, pol, tat, rev, nef, vif, vpu, vpr, gag/pol, or env-gag/pol sequences, or fragments or variants thereof, are also contemplated for use with the present invention. Any suitable viral vector that can be used to prepare recombinant vector/HIV or recombinant vector/SIV constructs is contemplated for use with the subject invention. For example, viral vectors derived from adenovirus, avipox, feline herpesvirus, vaccinia, canarypox, entomopox, swinepox and others known in the art can be used with the compositions and methods of the present invention. Recombinant polynucleotide vectors that encode and that can express HIV or SIV components in a suitable host cell can be constructed using standard genetic engineering techniques known in the art.
DNA or RNA vaccines which comprise nucleic acid that encodes one or more primate immunodeficiency virus proteins, or a fragment or variant thereof, are also contemplated for use in the subject invention. The nucleic acid can be provided in the form of a plasmid or eukaryotic expression construct. DNA vaccines and formulations have been described, for example, in published patent applications 20040076632; 20040047878; 20040009941; 20020156037; 20020032165; and 20010004531.
As used herein, the term “expression construct” refers to a combination of nucleic acid sequences that provides for transcription of an operably linked nucleic acid sequence. As used herein, the term “operably linked” refers to a juxtaposition of the components described wherein the components are in a relationship that permits them to function in their intended manner. In general, operably linked components are in contiguous relation.
Expression constructs of the invention will also generally include regulatory elements that are functional in the intended host cell in which the expression construct is to be expressed. Thus, a person of ordinary skill in the art can select regulatory elements for use in, for example, bacterial host cells, yeast host cells, plant host cells, insect host cells, mammalian host cells, and human host cells. Regulatory elements include promoters, transcription termination sequences, translation termination sequences, enhancers, and polyadenylation elements.
An expression construct of the invention can comprise a promoter sequence operably linked to a polynucleotide sequence encoding a peptide of the invention. Promoters can be incorporated into a polynucleotide using standard techniques known in the art. Multiple copies of promoters or multiple promoters can be used in an expression construct of the invention. In a preferred embodiment, a promoter can be positioned about the same distance from the transcription start site as it is from the transcription start site in its natural genetic environment. Some variation in this distance is permitted without substantial decrease in promoter activity. A transcription start site is typically included in the expression construct.
For expression in animal cells, an expression construct of the invention can comprise suitable promoters that can drive transcription of the polynucleotide sequence. If the cells are mammalian cells, then promoters such as, for example, actin promoter, metallothionein promoter, NF-kappaB promoter, EGR promoter, SRE promoter, IL-2 promoter, NFAT promoter, osteocalcin promoter, SV40 early promoter and SV40 late promoter, Lck promoter, BMP5 promoter, TRP-1 promoter, murine mammary tumor virus long terminal repeat promoter, STAT promoter, or an immunoglobulin promoter can be used in the expression construct.
Expression constructs of the invention may optionally contain a transcription termination sequence, a translation termination sequence, signal peptide sequence, and/or enhancer elements. Transcription termination regions can typically be obtained from the 3′ untranslated region of a eukaryotic or viral gene sequence. Transcription termination sequences can be positioned downstream of a coding sequence to provide for efficient termination. Signal peptides are a group of short amino terminal sequences that encode information responsible for the relocation of an operably linked peptide to a wide range of post-translational cellular destinations, ranging from a specific organelle compartment to sites of protein action and the extracellular environment. Targeting a peptide to an intended cellular and/or extracellular destination through the use of operably linked signal peptide sequence is contemplated for use with the immunogens of the invention. Chemical enhancers are cis-acting elements that increase gene transcription and can also be included in the expression construct. Chemical enhancer elements are known in the art, and include, but are not limited to, the cytomegalovirus (CMV) early promoter enhancer element and the SV40 enhancer element. DNA sequences which direct polyadenylation of the mRNA encoded by the structural gene can also be included in the expression construct.
Unique restriction enzyme sites can be included at the 5′ and 3′ ends of the expression construct to allow for insertion into a polynucleotide vector. As used herein, the term “vector” refers to any genetic element, including for example, plasmids, cosmids, chromosomes, phage, virus, and the like, which is capable of replication when associated with proper control elements and which can transfer polynucleotide sequences between cells. Vectors contain a nucleotide sequence that permits the vector to replicate in a selected host cell. A number of vectors are available for expression and/or cloning, and include, but are not limited to, pBR322, pUC series, M13 series, and pBLUESCRIPT vectors (Stratagene, La Jolla, Calif.).
Polynucleotides, vectors, and expression constructs of the invention can also be introduced in vivo via lipofection (DNA transfection via liposomes prepared from synthetic cationic lipids) (Felgner et al., 1987). Synthetic cationic lipids (LIPOFECTIN, Invitrogen Corp., La Jolla, Calif.) can be used to prepare liposomes to encapsulate a polynucleotide, vector, or expression construct of the invention. A polynucleotide, vector, or expression construct of the invention can also be introduced as naked DNA using methods known in the art, such as transfection, microinjection, electroporation, calcium phosphate precipitation, and by biolistic methods.
As used herein, the terms “nucleic acid” and “polynucleotide sequence” refer to a deoxyribonucleotide or ribonucleotide polymer in either single- or double-stranded form, and unless otherwise limited, would encompass known analogs of natural nucleotides that can function in a similar manner as naturally-occurring nucleotides. The polynucleotide sequences include both the DNA strand sequence that is transcribed into RNA and the RNA sequence that is translated into protein. The polynucleotide sequences include both full-length sequences as well as shorter sequences derived from the full-length sequences. It is understood that a particular polynucleotide sequence includes the degenerate codons of the native sequence or sequences which may be introduced to provide codon preference in a specific host cell. The polynucleotide sequences falling within the scope of the subject invention further include sequences which specifically hybridize with the exemplified sequences. The polynucleotide includes both the sense and antisense strands as either individual strands or in the duplex.
The methods of the present invention contemplate a primary immunization with a vaccine composition of the invention. Subsequent or secondary immunizations are also contemplated within the scope of the subject methods. The vaccine composition used for secondary immunizations can be the same as or vary from the composition used for primary immunization. For example, primary immunizations of an animal may use recombinant vector-based HIV or SIV constructs, having single or multiple strain components, followed by secondary boosts with vaccine compositions comprising HIV- or SIV-infected cell lines, or HIV or SIV polypeptides, or cell free HIV or SIV virus, also having single or multiple strain components. Primary immunizations can also use an HIV and/or SIV DNA vaccine. In one embodiment, a recombinant vector construct is used for the primary immunization, whereas a protein, or protein plus recombinant vector construct, subunit vaccine composition is used for secondary boosts. In addition, the subject invention contemplates methods wherein the immunogen(s) used for a primary immunization is an HIV or SIV derived immunogen, e.g., an HIV p24 protein, and the immunogen(s) used for secondary immunizations are derived from FIV, e.g., FIV gp95 and/or gp36 protein, or whole FIV virus, or FIV-infected cells. Other immunization protocols with the vaccine compositions of the invention are apparent to persons skilled in the art and are contemplated within the scope of the present invention.
In one embodiment, immunogens from various primate immunodeficiency viral proteins are combined for administration to an animal. For example, immunogens from any of HIV p7, p17, p24, gp41, gp120, and gp160 can be combined for administration. In an exemplified embodiment, a composition comprising an immunogen of HIV-1 p24 and an immunogen of HIV-1 gp120 and gp160 is administered to a feline animal. In another exemplified embodiment, a composition comprising HIV-1 p24 as the only immunogen is administered to a feline animal.
The subject invention also concerns materials and methods for protecting a feline animal against infection by an FIV or for generating an immune response against an FIV by administering to the animal a composition comprising immunogens derived from primate immunodeficiency viruses and immunogens derived from FIV. FIV-derived immunogens have been described in the art. See, for example, U.S. Pat. Nos. 5,118,602; 5,565,319; 5,510,106; 5,275,813; 5,037,753; and 4,861,720. FIV immunogens that can be used in accordance with the subject invention can be either of a single subtype or strain of FIV, or the immunogen can be derived from multiple subtypes of FIV. Multi-subtype FIV immunogens have been described in U.S. Pat. Nos. 5,846,825; 6,254,872; and 6,447,993.
In one embodiment of the subject methods, the immunogen to be administered to a feline animal comprises an epitope that is conserved between the primate immunodeficiency virus and FIV. In a further embodiment, the conserved epitope is present in the envelope protein of an immunodeficiency virus. Also contemplated within the scope of the invention are molecules (mimotope) which have a conformation that has a topology equivalent to a conserved epitope of the present invention and which binds to the same antigen-binding region of an antibody that binds to the conserved epitope. Typically, mimotopes are peptide molecules, but mimotopes can also be prepared from other non-peptide molecules.
The subject invention also concerns immunogens derived from a primate immunodeficiency virus, wherein the immunogen is a protein or peptide and comprises an epitope conserved between the primate immunodeficiency virus and FIV. In an exemplified embodiment, the immunogen comprises a gp120 and gp160 protein from HIV-1IIIB or an immunogenic fragment or variant thereof, and, optionally, a p24 protein from HIVUCD1, or an immunogenic fragment or variant thereof. In another embodiment, the immunogen comprises a p24 protein from HIVUCD1, or an immunogenic fragment or variant thereof. The subject invention also concerns polynucleotides that encode the protein or peptides comprising conserved epitopes of the immunodeficiency virus derived immunogens.
The subject invention also concerns antibodies that cross-react with epitopes of the immunogens derived from primate immunodeficiency viruses and FIV. The antibodies can be polyclonal or monoclonal in form. The antibodies can be derived from any animal capable of producing antibodies to the epitopes, and include, for example, human, ape, monkey, mouse, rat, goat, sheep, pig, cow, and feline animals. Also contemplated within the scope of the invention are non-human antibodies that cross-react with an epitope of the immunogen derived from a primate immunodeficiency virus and FIV but that have been “humanized” using standard procedures known in the art, such as those described in U.S. Pat. Nos. 5,530,101; 5,585,089; 5,693,762; 6,180,370; and 6,407,213.
Peptide and/or polypeptide immunogens of the present invention can also be provided in the form of a multiple antigenic peptide (MAP) construct. The preparation of MAP constructs has been described in Tam (1988). MAP constructs utilize a core matrix of lysine residues onto which multiple copies of an immunogen are synthesized (Posnett et al., 1988). Multiple MAP constructs, each containing the same or different immunogens, can be prepared and administered in a vaccine composition in accordance with methods of the present invention. In one embodiment, a MAP construct is provided with and/or administered with one or more adjuvants.
Natural, recombinant or synthetic polypeptides of primate immunodeficiency viral proteins, and peptide fragments thereof, can also be used as vaccine compositions according to the subject methods. In one embodiment, different polypeptides are administered as a combined preparation to a feline animal. In an exemplified embodiment, HIV-1 gp120/160 and HIV-1 p24 polypeptides are administered to a feline animal. In another embodiment, HIV polypeptides derived from more than one HIV strain are combined in a vaccine composition and are used to vaccinate a host animal. For example, polypeptides based on the HIV envelope glycoprotein from at least two strains of HIV-1 can be combined in the vaccine. The polypeptides may comprise “hybrid” or “chimeric” polypeptides whose amino acid sequence is derived from joining or linking polypeptides from at least two distinct HIV strains. Procedures for preparing HIV polypeptides are well known in the art. For example, HIV polypeptides can be synthesized using solid-phase synthesis methods (Merrifield, 1963). HIV polypeptides can also be produced using recombinant DNA techniques wherein a polynucleotide molecule encoding an HIV protein or peptide is expressed in a host cell, such as bacteria, yeast, or mammalian cell lines, and the expressed protein purified using standard techniques of the art.
According to the methods of the subject invention, the immunogenic compositions described herein are administered to susceptible hosts, typically domestic cats, in an effective amount and manner to induce protective immunity against subsequent challenge or infection of the host by FIV. The immunogens are typically administered parenterally, by injection, for example, either subcutaneously, intraperitoneally, or intramuscularly, or by oral or nasal administration, or any combination of such routes of administration. Usually, the immunogens are administered to a host animal at least two times, with an interval of one or more weeks between each administration. However, other regimens for the initial and booster administrations of the immunogens are contemplated, and may depend on the judgment of the practitioner and the particular host animal being treated.
Immunogens that can be used in accordance with the present invention can be provided with a pharmaceutically-acceptable carrier or diluent. Compounds and compositions useful in the subject invention can be formulated according to known methods for preparing pharmaceutically useful compositions. Formulations are described in detail in a number of sources which are well known and readily available to those skilled in the art. For example, Remington's Pharmaceutical Science by E. W. Martin, Easton Pa., Mack Publishing Company, 19th ed., 1995, describes formulations which can be used in connection with the subject invention. In general, the compositions of the subject invention will be formulated such that an effective amount of an immunogen is combined with a suitable carrier in order to facilitate effective administration of the composition. The compositions used in the present methods can also be in a variety of forms. These include, for example, solid, semi-solid, and liquid dosage forms, such as tablets, pills, powders, liquid solutions or suspension, suppositories, injectable and infusible solutions, and sprays. The preferred form depends on the intended mode of administration and therapeutic application. The compositions also preferably include conventional pharmaceutically acceptable carriers and diluents which are known to those skilled in the art. Examples of carriers or diluents for use with the subject peptidomimetics include, but are not limited to, water, saline, oils including mineral oil, ethanol, dimethyl sulfoxide, gelatin, cyclodextrans, magnesium stearate, dextrose, cellulose, sugars, calcium carbonate, glycerol, alumina, starch, and equivalent carriers and diluents, or mixtures of any of these. Formulations of an immunogen of the invention can also comprise suspension agents, protectants, lubricants, buffers, preservatives, and stabilizers. To provide for the administration of such dosages for the desired therapeutic treatment, pharmaceutical compositions of the invention will advantageously comprise between about 0.1% and 45%, and especially, 1 and 15% by weight of the immunogen or immunogens based on the weight of the total composition including carrier or diluent.
The immunogenic compositions of the subject invention can be prepared by procedures well known in the art. For example, the immunogens are typically prepared as injectables, e.g., liquid solutions or suspensions. The immunogens are administered in a manner that is compatible with dosage formulation, and in such amount as will be therapeutically effective and immunogenic in the recipient. The optimal dosages and administration patterns for a particular immunogens formulation can be readily determined by a person skilled in the art.
As noted above, virus and cells in a immunogenic formulation may be inactivated or attenuated using methods known in the art. The amount of cell-free whole or partial virus in a vaccine dose will usually be in the range from about 0.1 mg to about 5 mg, and more usually being from about 0.2 mg to about 2 mg. The dosage for formulations comprising virus-infected cell lines will usually contain from about 106 to about 108 cells per dose, and more usually from about 5×106 to about 7.5×107 cells per dose. The amount of protein or peptide immunogen in a dose for a feline animal can vary from about 0.1 μg to 10000 μg, or about 1 μg to 5000 μg, or about 10 μg to 1000 μg, or about 25 μg to 750 μg, or about 50 μg to 500 μg, or 100 μg to 250 μg, depending upon the size, age, etc., of the animal receiving the dose.
In one embodiment, an immunogen of the invention is provided with one or more adjuvants that increase the animal's immune response against the immunogen. Immunogens of the invention can be provided with and/or administered with any suitable adjuvant or adjuvants known in the art. In one embodiment, the adjuvant is one that helps induce a strong cellular immune response. Adjuvants that can be used in the immunogen formulations of the invention include threonyl muramyl dipeptide (MDP) (Byars et al., 1987), Ribi adjuvant system components (Corixa Corp., Seattle, Wash.) including the cell wall skeleton (CWS) component, Freund's complete, and Freund's incomplete adjuvants, bacterial lipopolysaccharide (LPS), such as from E. coli, or a combination thereof. A variety of other adjuvants suitable for use with the methods and vaccines of the subject invention, such as alum, aluminum hydroxide, and saponin are well known in the art and are contemplated for use with the subject invention. Cytokines (γ-IFN, GM-CSF, CSF, etc.) and lymphokines and interleukins (IL-1, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8. IL-9, IL-10, IL-11, IL-12, IL-13, IL-14, IL-15, IL-16, IL-17, IL-18, IL-19, IL-20, IL-21, and IL-22) have also been used as adjuvants and/or supplements to vaccine compositions and are contemplated within the scope of the present invention. One or more different cytokines and lymphokines can be included in a composition comprising an immunogen of the invention. In one embodiment, an immunogen of the invention is administered to an animal in combination with the lymphokine interleukin-12 (IL-12) in combination with another adjuvant. Also specifically contemplated within the scope of the invention is the use of the lymphokine interleukin-18 (IL-18) as part of an adjuvant composition. In one embodiment, an adjuvant composition used with the subject invention comprises a combination of IL-12 and IL-15, or IL-15 and IL-18, or IL-12 and IL-18, or IL-12, IL-15, and IL-18. The cytokine selected is of a species that has biological activity in the animal receiving the immunogen. For example, if the animal is a cat, then the cytokine can be a human cytokine or a feline cytokine, e.g., feline IL-12, feline IL-15, feline IL-18, etc. In an exemplified embodiment, a primate derived immunogen, such as HIV-1 p24, is administered in combination with Ribi adjuvant system component including CWS and IL-12 and/or IL-18.
Abbreviations of FIV strains used herein are shown below:
The subject invention also concerns methods of use of an animal model for selecting for epitopes conserved between immunodeficiency viruses, such as HIV and FIV, that can be used to immunize a person or animal against infection by an immunodeficiency virus. In one embodiment of the method, HIV is isolated from an HIV-infected, long-term nonprogressor patient. As used herein, the term “long-term nonprogressors” refers to HIV-infected patients that exhibit a stable CD4 count for at least 10 years, exhibit a low virus load (i.e., virus level in plasma is low to undetectable as measured by RT-PCR) and typically exhibit few or no disease symptoms. Immunogens from the isolated HIV are prepared and used to immunize a feline animal, such as a domestic cat. In one embodiment of the method, peptide fragments of an HIV protein are used as immunogens. Preferably, overlapping fragments that represent the full length of the HIV protein are prepared for use as immunogens. In one embodiment, different combinations of the peptide fragments are administered to different cats in order to identify those fragments that contain epitopes that provide the strongest prophylactic protection against FIV infection. Immunized cats are subsequently challenged with FIV. Immunogens that protect a cat against infection when challenged with FIV comprise evolutionarily conserved epitopes and can be used as immunogens to immunize humans, feline animals, and other mammals against infection by immunodeficiency viruses. Preferably, the immunogen protects a cat against infection by FIV of more than one subtype. Fragments of an immunogen comprising an evoluationarily conserved epitope can be prepared and tested using the subject method to further isolate the epitope. Fragments can also be sequenced to determine the primary amino acid sequence of the epitope. In a preferred embodiment, an immunogen comprising an evolutionarily conserved epitope selected using the subject method can be used to immunize a human against infection from HIV. In one embodiment of the method, the immunogen used to immunize cats is an HIV p24 protein, or an immunogenic fragment thereof. The subject invention also concerns evolutionarily conserved epitopes of immunodeficiency viruses identified using the subject method.
The subject invention also concerns methods for inducing an immune response in humans and other animals, such as cats and other felids, against immunogens, antigens, or viruses comprising epitopes that are evolutionarily conserved among immunodeficiency viruses, such as HIV and FIV. In one embodiment, an immunogen(s) or antigen(s) comprising one or more evolutionarily conserved epitope(s) from an immunodeficiency virus(es) identified by methods described herein is administered to a person or animal in an amount and for a duration sufficient to induce an immune response against the immunogen or antigen and any virus or cell displaying or having the immunogen or antigen. The immune response induced can be humoral or cell-mediated or both. In one embodiment for inducing an immune response against HIV, a human is administered an effective amount of an immunogen comprising an evolutionarily conserved epitope identified from an HIV. Immunogens contemplated within the scope of the invention include, but are not limited to, an HIV p24 protein, or an immunogenic fragment thereof. In one embodiment, an immunogen used in the present method comprises an HIV-1UCD1 p24 protein, or an immunogenic fragment thereof. In a specific embodiment, at least one immunogen is an HIV peptide of the invention comprising an amino acid sequence shown in any of SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, or SEQ ID NO: 39, or an immunogenic fragment or variant of SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, or SEQ ID NO: 39. In a further embodiment for inducing an immune response against HIV, a human is administered an immunogen comprising an evolutionarily conserved epitope identified from an FIV. In one embodiment, an immunogen used in the present method comprises an FIV p24 protein, or an immunogenic fragment thereof. In a specific embodiment, at least one immunogen is an FIV peptide of the invention comprising an amino acid sequence shown in any of SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 42, SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, or SEQ ID NO: 46, or an immunogenic fragment or variant of SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 42, SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, or SEQ ID NO: 46.
In one embodiment for inducing an immune response in a feline animal against FIV, the animal is administered an immunogen comprising an evolutionarily conserved epitope identified from an HIV. In one embodiment, an immunogen used in the present method comprises an HIV-1 p24 protein, or an immunogenic fragment thereof. In a specific embodiment, at least one immunogen is an HIV peptide of the invention comprising an amino acid sequence shown in any of SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, or SEQ ID NO: 39, or an immunogenic fragment or variant of SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, or SEQ ID NO: 39. In a further embodiment for inducing an immune response in a feline animal against FIV, the animal is administered an immunogen comprising an evolutionarily conserved epitope identified from an FIV. In one embodiment, an immunogen used in the present method comprises an FIV p24 protein, or an immunogenic fragment thereof. In a specific embodiment, at least one immunogen is an FIV peptide of the invention comprising an amino acid sequence shown in any of SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 42, SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, or SEQ ID NO: 46, or an immunogenic fragment or variant of SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 42, SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, or SEQ ID NO: 46.
The subject invention also concerns methods for treating and protecting humans, and other animals, such as cats and other felids, against infection by immunodeficiency viruses, such as HIV and FIV. In one embodiment of the method, an effective amount of an immunogen(s) or antigen(s) comprising one or more evolutionarily conserved epitope(s) from an immunodeficiency virus(es) identified by methods described herein is administered to a person or animal for a duration sufficient to immunize a person or animal and provide the person or animal with some level of protection against infection by an immunodeficiency virus. In one embodiment for treatment or protection against HIV infection, a human is immunized with an immunogen comprising an evolutionarily conserved epitope identified from an HIV. Immunogens contemplated within the scope of the invention include, but are not limited to, an HIV p24 protein, or an immunogenic fragment thereof. In one embodiment, an immunogen used in the present method comprises an HIV-1UCD1, HIV-1LAI/LAV, or HIV-1IIIB p24 protein, or an immunogenic fragment thereof. In a specific embodiment, at least one immunogen is an HIV peptide of the invention comprising an amino acid sequence shown in any of SEQ ID NO:9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, or SEQ ID NO: 39, or an immunogenic fragment or variant of SEQ ID NO:9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, or SEQ ID NO: 39. In another embodiment for treatment or protection against HIV infection, a person is immunized with an immunogen comprising an evolutionarily conserved epitope identified from an FIV. In one embodiment, an immunogen used in the present method comprises an FIV p24 protein, or an immunogenic fragment thereof. In a specific embodiment, at least one immunogen is an FIV peptide of the invention comprising an amino acid sequence shown in any of SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 42, SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, or SEQ ID NO: 46, or an immunogenic fragment or variant of SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 42, SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, or SEQ ID NO: 46.
In one embodiment for treating or protecting a feline animal from infection by FIV, the animal is administered an immunogen comprising an evolutionarily conserved epitope identified from an HIV. In one embodiment, an immunogen used in the present method comprises an HIV-1 p24 protein, or an immunogenic fragment thereof. In a specific embodiment, at least one immunogen is an HIV peptide of the invention comprising an amino acid sequence shown in any of SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, or SEQ ID NO: 39, or an immunogenic fragment or variant of SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, or SEQ ID NO: 39. In a further embodiment for protecting a feline animal from infection by FIV, the animal is administered an immunogen comprising an evolutionarily conserved epitope identified from an FIV. In one embodiment, an immunogen used in the present method comprises an FIV p24 protein, or an immunogenic fragment thereof. In a specific embodiment, at least one immunogen is an FIV peptide of the invention comprising an amino acid sequence shown in any of SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 42, SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, or SEQ ID NO: 46, or an immunogenic fragment or variant of SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 42, SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, or SEQ ID NO: 46.
The subject invention also concerns FIV peptides representing epitopes conserved with primate immunodeficiency viruses, such as HIV and SIV. In one embodiment, the FIV peptide comprises an amino acid sequence from an FIV p24 protein, or an immunogenic fragment or variant thereof. In a specific embodiment, the FIV p24 protein is an FIVBANG, FIVSHI, or FIVFC1 p24 protein. In an exemplified embodiment, an FIV peptide of the invention has an amino acid sequence shown in any of SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 42, SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, or SEQ ID NO: 46, or an immunogenic fragment or variant of SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 42, SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, or SEQ ID NO: 46.
The subject invention also concerns HIV peptides representing epitopes conserved with feline immunodeficiency viruses. In one embodiment, the HIV peptide comprises an amino acid sequence from an HIV p24 protein, or an immunogenic fragment or variant thereof. In a specific embodiment, the HIV p24 protein is an HIV-1UCD1 or an HIV-1LAI/LAV p24 protein. In an exemplified embodiment, an HIV peptide of the invention has an amino acid sequence shown in any of SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, or SEQ ID NO: 39, or an immunogenic fragment or variant of SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, or SEQ ID NO: 39.
Immunogens of the invention are typically administered parenterally, by injection, for example, either subcutaneously, intraperitoneally, or intramuscularly. Other suitable modes of administration include oral or nasal administration. Usually, the immunogens are administered to a human or other animal at least two times, with an interval of one or more weeks between each administration. However, other regimens for the initial and booster administrations of the immunogens are contemplated, and may depend on the judgment of the practitioner and the patient being treated.
Immunogenic compositions of the subject invention can be prepared by procedures well known in the art. For example, the immunogens are typically prepared as injectables, e.g., liquid solutions or suspensions. The immunogens are administered in a manner that is compatible with dosage formulation, and in such amount as will be therapeutically effective and immunogenic in the recipient. The optimal dosages and administration patterns for a particular immunogens formulation can be readily determined by a person skilled in the art.
Immunogens that can be used in accordance with the present invention can be provided with a pharmaceutically-acceptable carrier or diluent. In one embodiment, an immunogen of the invention is provided with one or more adjuvants that increase the human or animal's immune response against the immunogen. Immunogens of the invention can be provided with and/or administered with any suitable adjuvant or adjuvants known in the art.
The subject invention also concerns compositions comprising an immunogen of the invention derived from a primate immunodeficiency virus and a feline or human cytokine or lymphokine. The immunogen can be from HIV, including HIV-1, e.g., HIV-1IIIB, HIV-1LAI/LAV, HIV-1UCD1, and HIV-1BRU, and HIV-2. HIV immunogens of the composition include, but are not limited to, HIV gp160, gp120, gp41, p24, p31, p17, p7 or a protein encoded by an HIV gag, pol, env, tat, rev, nef, vif, vpr, vpu, or vpx genes, or a fragment or variant thereof. In a specific embodiment, an HIV peptide of the invention has an amino acid sequence shown in any of SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, or SEQ ID NO: 39, or an immunogenic fragment or variant of SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, or SEQ ID NO: 39. In one embodiment, the lymphokine is feline or human IL-12, IL-15, and/or IL-18. In an exemplified embodiment, the immunogen is HIV-1 p24 and the lymphokine is feline IL-18. Compositions can also include an adjuvant, such as one of the adjuvants described herein.
The subject invention also concerns compositions comprising an immunogen of the invention derived from a feline immunodeficiency virus and a feline or human cytokine or lymphokine. The immunogen can be from any subtype or strain of FIV, including FIVPet, FIVBang, and FIVshi. FIV immunogens of the composition include, but are not limited to, FIV gp160, gp120, gp41, p24, p31, p17, p7 or a protein encoded by an FIV gag, pol, env, tat, rev, nef, vif, vpr, vpu, or vpx genes, or a fragment or variant thereof. In a specific embodiment, an FIV peptide of the invention has an amino acid sequence shown in any of SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 42, SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, or SEQ ID NO: 46, or an immunogenic fragment or variant of SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 42, SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, or SEQ ID NO: 46. In one embodiment, the lymphokine is feline or human IL-12, IL-15, and/or IL-18. In a specific embodiment, the immunogen is FIV-1 p24 and the lymphokine is human IL-18. Compositions can also include an adjuvant, such as one of the adjuvants described herein.
The subject invention also concerns kits and dosage formulations comprising in one or more containers an immunogen of the invention derived from a primate immunodeficiency virus and a feline or human cytokine or lymphokine. The immunogen can be from HIV, including HIV-1, e.g., HIV-1IIIB, HIV-1LAI/LAV, HIV-1UCD1, and HIV-1BRU, and HIV-2. HIV immunogens of the composition include, but are not limited to, HIV gp160, gp120, gp41, p24, p31, or a protein encoded by an HIV gag, pol, env, tat, rev, nef, vif, vpr, vpu, or vpx gene, or an immunogenic fragment or variant thereof. In an exemplified embodiment, an HIV peptide of the invention has an amino acid sequence shown in any of SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, or SEQ ID NO: 39, or an immunogenic fragment or variant of SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, or SEQ ID NO: 39. In one embodiment, the lymphokine is feline or human IL-12, IL-15, and/or IL-18. In an exemplified embodiment, the immunogen is HIV-1 p24 and the lymphokine is feline IL-18. Kits and dosage formulations can also include an adjuvant, such as one of the adjuvants described herein.
The subject invention also concerns kits and dosage formulations comprising in one or more containers an immunogen of the invention derived from a feline immunodeficiency virus and a feline or human cytokine or lymphokine. The immunogen can be from any subtype or strain of FIV, including FIVPet, FIVBang, and FIVShi. FIV immunogens of the composition include, but are not limited to, FIV gp160, gp120, gp41, p24, p31, p17, p7, or a protein encoded by an FIV gag, pol, env, tat, rev, nef, vif, vpr, vpu, or vpx gene, or an immunogenic fragment or variant thereof. In an exemplified embodiment, an FIV peptide of the invention has an amino acid sequence shown in any of SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 42, SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, or SEQ ID NO: 46, or an immunogenic fragment or variant of SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 42, SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, or SEQ ID NO: 46. In one embodiment, the lymphokine is feline or human IL-12, IL-15, and/or IL-18. In a specific embodiment, the immunogen is FIV p24 and the lymphokine is human IL-18. Kits and dosage formulations can also include an adjuvant, such as one of the adjuvants described herein.
The peptides contemplated in the subject invention include the specific peptides exemplified herein as well as equivalent peptides which may be, for example, somewhat longer or shorter than the peptides exemplified herein. For example, using the teachings provided herein, a person skilled in the art could readily make peptides having from 1 to about 15 or more amino acids added to, or 1 to 3 amino acids removed from, either or both ends of the disclosed peptides using standard techniques known in the art. Preferably, any added amino acids would be the same as the corresponding amino acids of the full-length protein from which the peptide is derived. The skilled artisan, having the benefit of the teachings disclosed in the subject application, could easily determine whether a longer or shorter peptide retained the immunogenic activity of the specific peptides exemplified herein.
Substitution of amino acids other than those specifically exemplified or naturally present in a peptide of the invention are also contemplated within the scope of the present invention. For example, non-natural amino acids can be substituted for the amino acids of a peptide, so long as the peptide having the substituted amino acids retains substantially the same immunogenic activity as the peptide in which amino acids have not been substituted. Examples of non-natural amino acids include, but are not limited to, ornithine, citrulline, hydroxyproline, homoserine, phenylglycine, taurine, iodotyrosine, 2,4-diaminobutyric acid, α-amino isobutyric acid, 4-aminobutyric acid, 2-amino butyric acid, γ-amino butyric acid, ε-amino hexanoic acid, 6-amino hexanoic acid, 2-amino isobutyric acid, 3-amino propionic acid, norleucine, norvaline, sarcosine, homocitrulline, cysteic acid, τ-butylglycine, τ-butylalanine, phenylglycine, cyclohexylalanine, β-alanine, fluoro-amino acids, designer amino acids such as β-methyl amino acids, C-methyl amino acids, N-methyl amino acids, and amino acid analogues in general. Non-natural amino acids also include amino acids having derivatized side groups. Furthermore, any of the amino acids in the protein can be of the D (dextrorotary) form or L (levorotary) form.
Amino acids can be generally categorized in the following classes: non-polar, uncharged polar, basic, and acidic. Conservative substitutions whereby a peptide of the present invention having an amino acid of one class is replaced with another amino acid of the same class fall within the scope of the subject invention so long as the peptide having the substitution still retains substantially the same immunogenic activity as the peptide that does not have the substitution. Table 1 below provides a listing of examples of amino acids belonging to each class.
Polynucleotides encoding a specifically exemplified peptide of the invention, or a shorter or longer peptide, or a peptide having one or more amino acid substitutions in the sequence are contemplated within the scope of the present invention. The subject invention also concerns variants of the polynucleotides of the present invention that encode a peptide of the invention. Variant sequences include those sequences wherein one or more nucleotides of the sequence have been substituted, deleted, and/or inserted. The nucleotides that can be substituted for natural nucleotides of DNA have a base moiety that can include, but is not limited to, inosine, 5-fluorouracil, 5-bromouracil, hypoxanthine, 1-methylguanine, 5-methylcytosine, and tritylated bases. The sugar moiety of the nucleotide in a sequence can also be modified and includes, but is not limited to, arabinose, xylulose, and hexose. In addition, the adenine, cytosine, guanine, thymine, and uracil bases of the nucleotides can be modified with acetyl, methyl, and/or thio groups. Sequences containing nucleotide substitutions, deletions, and/or insertions can be prepared and tested using standard techniques known in the art.
Fragments and variants of a peptide of the present invention can be generated as described herein and tested for the presence of immunogenic activity using standard techniques known in the art.
Polynucleotides and peptides contemplated within the scope of the subject invention can also be defined in terms of more particular identity and/or similarity ranges with those sequences of the invention specifically exemplified herein. The sequence identity will typically be greater than 60%, preferably greater than 75%, more preferably greater than 80%, even more preferably greater than 90%, and can be greater than 95%. The identity and/or similarity of a sequence can be 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, or 99% as compared to a sequence exemplified herein. Unless otherwise specified, as used herein percent sequence identity and/or similarity of two sequences can be determined using the algorithm of Karlin and Altschul (1990), modified as in Karlin and Altschul (1993). Such an algorithm is incorporated into the NBLAST and XBLAST programs of Altschul et al. (1990). BLAST searches can be performed with the NBLAST program, score=100, wordlength=12, to obtain sequences with the desired percent sequence identity. To obtain gapped alignments for comparison purposes, Gapped BLAST can be used as described in Altschul et al. (1997). When utilizing BLAST and Gapped BLAST programs, the default parameters of the respective programs (NBLAST and XBLAST) can be used. See Worldwide Website: ncbi.nlm.nih.gov.
All patents, patent applications, provisional applications, and publications referred to or cited herein are incorporated by reference in their entirety to the extent they are not inconsistent with the explicit teachings of this specification.
Vaccine Efficacy of Studies 1-4.
Four separate studies were performed to evaluate the protective efficacy of HIV-1 p24 vaccine against FIVBang and FIVFC1 strains in cats (Table 2, Studies 1-4). FIVFC1 belongs to subtype B and FIVBang is a recombinant of subtype Agag,pol,env(V1-V3) and subtype Benv(V4-V9) (Pu et al., 2005). The p24 from subtype B HIV-1 (HIV-1UCD1 or HIV-1LAI/LAV) that cross-reacted strongly with sera from dual-subtype FIV vaccinated cats was used as vaccine immunogen (Pu et al., 2001). The HIV-1 p24 vaccine consisted of recombinant p24 (200-250 μg) of HIV-1UCD1 or HIV-1LAI/LAV mixed in modified Ribi adjuvant (Corixa Corporation, Hamilton, Mont.) (1 ml/dose) containing recombinant human interleukin-12 (rHuIL-12, Genetics Institute, Cambridge, Mass.; 5 μg/dose) (Ribi/rHuIL-12), recombinant feline IL-18 (rFeIL-18; 5 μg/dose) (Ribi/rFeIL-18), or without cytokine (Ribi). The modified Ribi adjuvant (modification of Corixa's Ribi R-730) contained cell wall skeleton (25 μg/dose) (Corixa Corporation. Ribi Adjuvant System (RAS) research adjuvant fact sheet. http://www.corixa.com/ras.pdf) and E. coli M15 lipopolysaccharide (5-50 EU/dose) as our approach to enhance toll-receptor recognition (Reis e Sousa, 2004). In Study 1, the cats were immunized with either HIV-1UCD1 p24 in Ribi/rHuIL-12 (Group 1), HIV-1LAI/LAV p24 in Ribi/rHuIL-12 (Group 2), HIV-1UCD1 p24 in Ribi (Group 3), or Ribi alone (Group 4). In Study 2, the cats were immunized with either HIV-1LAI/LAV p24 in Ribi/rHuIL-12 (Group 1), FIVBang p24 in Ribi/rHuIL-12 (Group 2), FIVPet/Shi p24 in Ribi/rHuIL-12 (Group 3), or Ribi/rHuIL-12 (Group 4). In Study 3, the cats were immunized with HIV-1UCD1 p24 in Ribi/rFeIL-18 (Group 1) or Ribi (Group 2); and the control cats were immunized with Ribi/rFeIL-18, Ribi, or PBS (Group 3). In Study 4, the cats were immunized with HIV-1UCD1 p24 in Ribi/rHuIL-12 (Group 1) or with PBS (Group 2). These studies with individual groups are shown in Table 2.
All cats were challenged intravenously with 15 median cat infectious dose (CID50) of inoculum consisting of either pooled FIVBang-infected plasma (Studies 1-3) or pooled FIVFC1-infected peripheral blood mononuclear cells (PBMC) (Study 4) derived directly from infected animals (Pu et al., 2001). Infection status of the cats was monitored by virus isolation based on RT activity and proviral PCR performed on the PBMC collected every 3-4 weeks post-inoculation (wpi) and on the lymph node (LN) and bone marrow (BM) cells at 18 or 24 wpi as previously described (Pu et al., 2001) or at 33-34 and 52 wpi. Cats were considered FIV negative by the absence of detectable virus, virus neutralizing (VN) antibodies, and immunoblot antibodies to FIV (nucleoprotein/matrix [p10/p15], polymerase [p65, p50], transmembrane envelope [gp40], and surface envelope [gp95]) at 1:50 and 1:250 serum dilutions (Pu et al., 2001). Immunoblot analysis was also used to detect cross-reactive antibodies to FIV core p24. All cats were monitored for 18 or 24 wpi, except for the vaccinated/protected cats from Studies 1, 3, and 4, which were monitored until 52 wpi.
Vaccine Immunogenicity
The FIVBang p24 reactivity of the antibodies from vaccine-immunized cats and adjuvant/PBS-immunized controls were determined by immunoblot analysis using FIVBang p24 as a substrate. The cross-reactive specificity of these antibodies (1:200 serum dilution) was determined by ELISA analysis (Yamomoto et al., 1993) using overlapping 28-30mer peptides of FIVBang p24 as substrates. Only those reactivities that are 3-fold the pre-immunization/pre-infection sera were considered positive reactivity. The FIV/HIV-1 specific cellular immune responses were determined by feline interferon-γ (FeIFNγ) ELISpot of dendritic cell (DC)-primed PBMC from vaccine-immunized and PBS-immunized cats. Briefly, plastic adherent BM cells were cultured in RPMI media containing recombinant feline granulocyte-macrophage colony-stimulating factor (50 ng/ml; R&D Systems, Minneapolis, Minn.) and rFeIL-4 (25 ng/ml) produced according to a previously described method (Lerner et al., 1997; Wondimu et al., 2001). On culture day 10, the non-adherent fraction was re-cultured for additional 2 days in RPMI media containing recombinant human tumor necrosis factor-α (50 ng/ml, R&D Systems), to induce maturation (Weigel et al., 2002). The mature DCs were then incubated with peptide (2 μg/0.2 μl/well) in 96 round-bottom wells (2×104 cells/well) for 18 hr and transferred to 96-well FeIFNγ ELISpot plates containing 2×105 autologous PBMC. ELISpot plates were processed according to manufacturer's method (R&D Systems) and analyzed with an ELISpot reader (MVS Pacific, LLC, Minneapolis, Minn.).
Sequencing, Expression, and Purification of HIV-1 p24, FIV p24, and FeIL-18
The proviral genes of HIV-1LAI/LAV (kindly provided by Dr. Francious Barre-Sinoussi, Pasteur Institute, France; NCBI accession #K02013), HIV-1UCD1 (isolated from an HIV-positive individual from San Diego, Calif.; NCBI #AY679786), FIVBang (NCBI #AY684181), FIVPet (NCBI #NC001482), and FIVShi (NCBI #D37818; #AY679785) were used to derive the viral p24 proteins. The whole gag sequence was obtained by PCR amplification of proviral DNA from HIV-1UCD1- or HIV-1LAI/LAV-infected HuT-78 cells, and cloned into pCR2.1 vector (TA cloning kit, Invitrogen, Carlsbad, Calif.). Three to eight clones were used for sequence confirmation. The HIV-1LAI/LAV p24 sequence was identical to the reported HIV-1HXB2 p24 sequence (NCBI #K03455) and differed from HIV-1UCD1 by eight aa. The confirmed sequence of p24 gene was subcloned into pQE30 for expression in E. coli M15[pREP4] (QIAexpressionist, Qiagen Inc., Valencia, Calif.). Recombinant p24 product was purified on Ni-NTA resin using the manufacturer's protocol. Purity of the product was >97% as determined by silver stain analysis on SDS-PAGE. The specificity of the product was determined by immunoblot analysis using human and feline polyclonal antibodies reactive to either HIV-1 or FIV p24, respectively.
The rFeIL-18 was produced from E. coli expression system using a previously described method with modification (Ishizaka et al., 2001; Salek-Ardakani et al., 2002). The FeIL-18 sequence was obtained by RT-PCR amplification of mRNA from feline splenocytes. Amplified products were cloned into pCR2.1 vector for sequence confirmation based on published FeIL-18 sequence (Ishizaka et al., 2001). The confirmed sequence was subcloned into pMAL-c2G (New England Biolabs, Inc., Beverly, Mass.) for expression in E. coli ER2508 strain as a fusion protein (FeIL-18 fused to maltose-binding protein). The fusion protein was cleaved with Genenase I and the cleaved product was purified by amylose resin chromatography using the manufacturer's protocol (New England Biolabs, Inc.). Purity of the product was determined by silver stain analysis. Biological activity of the rFeIL-18 was determined by proliferation-based IL-18 bioassay using PBMC instead of spleen cells (Gaucher et al., 2003).
Statistical Analyses
Individual immunization groups in each study (Table 2) and combined groups from different studies (Table 3) were analyzed for statistical significant difference by analysis of variance (ANOVA). Comparisons that demonstrated overall significance by ANOVA were evaluated by two-way paired T-test (SAS program, version 8.0) and were considered to have statistical difference when p<0.05.
Following are examples which illustrate procedures for practicing the subject invention. These examples should not be construed as limiting. All percentages are by weight and all solvent mixture proportions are by volume unless otherwise noted.
Four SPF cats each (n=4) were immunized four times with either HIV-1 vaccine formulation A or HIV-1 vaccine formulation B at 3 week intervals and challenged 3 weeks after the last boost with 15 cat infectious doses (CID50) of in vivo-derived FIVBangston innoculum. Additional four SPF cats (control group) received no immunization but were similarly challenged with FIV. HIV-1 vaccine formulation A consisted of 200 μg of HIV-1UCD1 p24, 25 μg of HIV-1IIIB gp120 (ImmunoDiagnostics Inc., Woburn, Mass.), and 25 μg of HIV-1IIIB gp160 (ImmunoDiagnostics Inc.) mixed in 0.5 ml of Ribi Adjuvant System comprising the cell wall skeleton (CWS) component (25 μg/dose cell wall skeleton)(Corixa Corp., Seattle, Wash.) containing 5 μg of recombinant human interleukin-12 (rHuIL-12). HIV-1 vaccine formulation B consisted of 200 μg of HIV-1UCD1 p24 mixed in 0.5 ml of Ribi Adjuvant System CWS component containing 5 μg of rHuIL-12. Recombinant HIV-1UCD1 p24 was produced in an E. coli expression system using QIAexpress pQE vector (Qiagen Inc., Valencia, Calif.) and the expressed 6×His-tagged p24 product was purified by Ni-NTA affinity chromatography (Qiagen Inc.). In vivo-derived FIV innoculum consisted of pooled plasma from two FIVBangston-infected cats which was titrated in vivo using groups of 3-4 SPF cats per log10 dilution (Pu et al., 2001).
Results of this study are shown in Table 1. As an additional method of determining FIV infection status, virus isolation was performed using both reverse transcriptase (RT) assay with proviral PCR as virus detection systems. One of four (1/4) cats immunized with HIV-1 vaccine formulation A and two of four (2/4) cats immunized with HIV-1 vaccine formulation B and subsequently challenged with FIV were negative for FIV by virus isolate, lost antibodies to FIV p24 and developed no antibodies to FIV gp95 at 16 weeks post challenge, while the remaining cats developed antibodies to FIV gp95 and developed persistently stronger antibody responses to FIV p24. All four control cats developed antibodies to both FIV p24 and gp95 by 16 weeks post challenge and virus isolation positive for FIV by 13 weeks post challenge. Hence, HIV-1 vaccine formulation B (HIV-1 p24 alone) appeared to have better or equivalent efficacy at protecting cats against FIV challenge than HIV-1 vaccine formulation A, suggesting that HIV-1UCD1 p24 is a vaccine component that can induce protective immunity against FIV challenge. All four cats immunized with HIV-1 vaccine formulation A and three of four cats immunized with HIV-1 vaccine formulation B developed cross-reactive antibodies to FIV p24 after the fourth (4th) vaccination.
Since there were no cross-reactive antibodies to FIV gp95 in HIV-1 vaccinated cats that exhibited a protective immune response to FIV, the absence or presence of antibodies to FIV gp95 was indicative of whether an animal had been vaccinated with HIV or infected with FIV, respectively. In addition, the loss of cross-reactive antibodies to FIV p24 in vaccinated cats by the end of the study was also indicative that the vaccinated animals were protected from FIV challenge.
In Study 1 (Table 2), all four cats (100%, Group 1) vaccinated with HIV-1UCD1 p24 in Ribi/rHuIL-12 were protected against FIVBang challenge that infected all three control cats (Group 4). In contrast, only two of four cats (50%, Group 2) vaccinated with HIV-1LAI/LAV p24 in Ribi/rHuIL-12, and one of three cats (33%, Group 3) vaccinated with HIV-1UCD1 p24 in Ribi were protected. Statistically significant difference was only observed between the protection rates of Groups 1 and 4 (p<0.01). All vaccinated/protected cats in Study 1 remained negative for FIV infection even at 52 wpi. In Study 2, all three cats (100%, Group 1) vaccinated with HIV-1LAI/LAV p24 in Ribi/rHuIL-12 were protected, while all three Ribi/rHuIL-12-immunized cats (Group 4) were infected. However, only one of three FIVBang p24-vaccinated cats (33%, Group 2) and two of three FIVPet/Shi p24-vaccinated cats (67%, Group 3) were protected.
Using a different T-helper 1 cell (Th1)-promoting cytokine adjuvant, in Study 3, two of three cats (67%, Group 1) vaccinated with HIV-1UCD1 p24 mixed in Ribi/rFeIL-18 were protected (
ELISA- and immunoblot-antibody titers to HIV-1 and FIV p24 proteins in Studies 1-4 increased with each HIV-1 p24 immunization (only immunoblot results shown,
The HIV-1LAI/LAV p24 sequence was identical to the reported HIV-1HXB2 p24 sequence (NCBI #K03455) and differed from HIV-1UCD1 p24 by eight aa (
A selected number of cats from Studies 3 and 4 were tested for cellular immune function (FeIFNγ ELISpot) after challenge and after demonstration of full protection at 33-34 wpi. Since the DC-priming was unavailable in our laboratory until recently, only three vaccinated/protected cats (#JB6 and #IW1, Study 3; #MF3, Study 4) and two infected control cats (#MK4 and #MG5, Study 4) were tested by FeIFNγ ELISpot analysis of the DC-primed PBMC. The PBMC from all three vaccinated/protected cats had strong FeIFNγ ELISpot responses to three FIV p24 peptides (F4.5/122-131, F6/155-164, F7/183-191) (≧baseline, FIG. 3B1). Two of these cats also responded to peptide F7.8/210-219. Furthermore, the PBMC from all three cats responded to the three counterpart HIV-1 p24 peptides (H4.5/130-140, H6/162-172, H7/191-199) and also to peptide H7.8/217-226 (≧baseline, FIG. 3B2). In contrast, the PBMC from infected control cat (#MK4) had only minimal FeIFNγ ELISpot responses to three FIV p24 peptides (F2/15-25, F7.8/210-219, F8/214-223) (≧baseline, FIG. 3B3), while the PBMC from another infected cat (#MG5) had no responses to any of the nine FIV p24 peptides (HIV-1 peptides not tested). Since these targeted peptides corresponded to HIV-1 sequences that are the reported CTL epitopes for HIV-1-positive individuals (Los Alamos National Laboratory. HIV Databases. Epitope maps. http://hiv-web.lanl.gov/content/immunology/maps/maps.html/), we speculate that they may represent FIV-specific CTL epitopes. Future studies using separated CD4+ and CD8+ T-cell subpopulations from vaccinated cats will determine whether the responses are CTL, Th, or both.
It should be understood that the examples and embodiments described herein are for illustrative purposes only and that various modifications or changes in light thereof will be suggested to persons skilled in the art and are to be included within the spirit and purview of this application. In addition, any elements or limitations of any invention or embodiment thereof disclosed herein can be combined with any and/or all other elements or limitations (individually or in any combination) or any other invention or embodiment thereof disclosed herein, and all such combinations are contemplated with the scope of the invention without limitation thereto.
aDetailed descriptions of the groups in Studies 1-4 are included in the Methods. The total number of cats in each study group is shown on the last column as # Total.
bBased on ANOVA, the protection rates between groups in only Study 1 had statistically significant differences. As a result, T-tests were performed between the protection rates of HIV-1 p24-vaccinated groups vs. the control group from Study 1 (i.e., Groups 1 vs. 4, Groups 2 vs. 4, Groups 3 vs. 4). Comparison between only Groups 1 and 4 had statistical significance and is shown with (p < 0.01) next to the protection rate.
cTwo cats each were immunized with either Ribi/rFeIL-18 (n = 2), Ribi (n = 2), or PBS (n = 2).
aThe combined protection rates and the statistical analyses performed on the combined groups from different studies are presented in Table 2. Only groups with similar vaccine formulations are combined. The first column shows the groups from different studies that were combined to obtain the different combined vaccinated groups and their corresponding combined control groups.
bBy combining the groups with similar vaccine formulations, statistically significant differences were achieved between the protection rates of the vaccinated (HIV-1 p24/Ribi/cytokine, FIV p24/Ribi/rHuIL-12) groups and the corresponding combined control (PBS, Ribi, Ribi/cytokine) groups by ANOVA, but not all comparisons were statistically significant by two-way paired T-test. Based on paired T-test, statistical differences were observed between the protection rates of the combined Groups A1 vs. A2 (p < 0.001); Groups B1 vs. B2 (p < 0.001); and Groups C1 vs. C2 (p < 0.01); but not between Groups D1 vs. D2 (p = 0.113) and Groups E1 vs. E2 (p = 0.170). Furthermore, except for the comparisons between Groups A1 vs. D1 (p = 0.048) and Groups B1 vs. D1 (p = 0.049), there were no statistically significant differences amongst the protections rates of the different p24-vaccinated groups (i.e., Group A1 vs. Group B1 vs. Group C1 vs. Group D1 vs. Group E1), including comparisons between the protection rates of Groups A1 vs. E1 (p = 0.211) and Groups B1 vs. E1 (p = 0.191). Those comparisons with statistical significance at p < 0.05 are shown with (*).
cThe protection rates of only Ribi-immunized (n = 2) and PBS-immunized (n = 2) cats in Study 3 were used (i.e., protection rate of Ribi/rFeIL-18-immunized cats not used). When the four Ribi/PBS-immunized cats from Study 3 are combined with the three Ribi-immunized cats from Study 1, the total number of control cats in Group D2 is seven (n = 7).
This application is a continuation-in-part of U.S. application Ser. No. 10/844,658, filed May 12, 2004, which claims the benefit of U.S. Provisional Application Ser. No. 60/470,066, filed May 12, 2003, each of which are hereby incorporated by reference in its entirety, including all figures, nucleic acid sequences, amino acid sequences, and tables.
The subject invention was made with government support under a research project supported by National Institutes of Health Grant No. NIH AI30904. The government has certain rights in this invention.
| Number | Name | Date | Kind |
|---|---|---|---|
| 4861720 | Pedersen et al. | Aug 1989 | A |
| 5037753 | Pedersen et al. | Aug 1991 | A |
| 5118602 | Pedersen et al. | Jun 1992 | A |
| 5275813 | Yamamoto et al. | Jan 1994 | A |
| 5401628 | Chiodi | Mar 1995 | A |
| 5510106 | Yamamoto et al. | Apr 1996 | A |
| 5530101 | Queen et al. | Jun 1996 | A |
| 5565319 | Pedersen et al. | Oct 1996 | A |
| 5585089 | Queen et al. | Dec 1996 | A |
| 5693762 | Queen et al. | Dec 1997 | A |
| 5700469 | McMichael et al. | Dec 1997 | A |
| 5763160 | Wang | Jun 1998 | A |
| 5846546 | Hurwitz et al. | Dec 1998 | A |
| 5846825 | Yamamoto | Dec 1998 | A |
| 5849533 | Berman et al. | Dec 1998 | A |
| 6107077 | Yamamoto | Aug 2000 | A |
| 6180370 | Queen et al. | Jan 2001 | B1 |
| 6254872 | Yamamoto | Jul 2001 | B1 |
| 6407213 | Carter et al. | Jun 2002 | B1 |
| 6447993 | Yamamoto | Sep 2002 | B1 |
| 6500623 | Tung | Dec 2002 | B1 |
| 6503753 | Rios | Jan 2003 | B1 |
| 6544528 | Yamamoto | Apr 2003 | B1 |
| 20010004531 | Sung et al. | Jun 2001 | A1 |
| 20020032165 | Johnson et al. | Mar 2002 | A1 |
| 20020156037 | Volkin et al. | Oct 2002 | A1 |
| 20030104611 | Johnston et al. | Jun 2003 | A1 |
| 20030223964 | Barnett et al. | Dec 2003 | A1 |
| 20040009941 | Johnson et al. | Jan 2004 | A1 |
| 20040047878 | Deng et al. | Mar 2004 | A1 |
| 20040076632 | Deng et al. | Apr 2004 | A1 |
| 20050031639 | Yamamoto | Feb 2005 | A1 |
| Number | Date | Country |
|---|---|---|
| WO 9301278 | Jan 1993 | WO |
| WO 9420622 | Sep 1994 | WO |
| WO 9428929 | Dec 1994 | WO |
| WO 9630045 | Oct 1996 | WO |
| WO 02067984 | Sep 2002 | WO |
| Number | Date | Country | |
|---|---|---|---|
| 20060147467 A1 | Jul 2006 | US |
| Number | Date | Country | |
|---|---|---|---|
| 60470066 | May 2003 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | 10844658 | May 2004 | US |
| Child | 11326062 | US |
