Claims
- 1. A substantially pure protein having the amino acid sequence of SEQ ID NO: 5 or SEQ ID NO: 7.
- 2. The protein of claim 1 wherein said protein has the amino acid sequence of SEQ ID NO: 5.
- 3. The protein of claim 1 wherein said protein has the amino acid sequence of SEQ ID NO: 7.
- 4. A pharmaceutical composition comprising the protein of claim 1 and a pharmaceutically acceptable carrier.
- 5. An isolated nucleic acid molecule that comprises a nucleic acid sequence that encodes the protein of claim 1.
- 6. A pharmaceutical composition comprising the nucleic acid molecule of claim 5 and a pharmaceutically acceptable carrier.
- 7. An isolated nucleic acid molecule consisting of SEQ ID NO: 4 or SEQ ID NO: 5 or a fragment thereof having at least 10 nucleotides.
- 8. The nucleic acid molecule of claim 7 consisting of SEQ ID NO: 4 or SEQ ID NO: 6.
- 9. A recombinant expression vector comprising the nucleic acid molecule of claim 8.
- 10. A host cell comprising the recombinant expression vector of claim 9.
- 11. The nucleic acid molecule of claim 7 consisting of a fragment of SEQ ID NO: 4 or SEQ ID NO: 6 having at least 10 nucleotides.
- 12. The nucleic acid molecule of claim 7 consisting of a fragment of SEQ ID NO: 4 or SEQ ID NO: 6 having 12-150 nucleotides.
- 13. The nucleic acid molecule of claim 7 consisting of a fragment of SEQ ID NO: 4 or SEQ ID NO: 6 having 15-50 nucleotides.
- 14. An oligonucleotide molecule comprising a nucleotide sequence complimentary to a nucleotide sequence of at least 5 nucleotides of SEQ ID NO: 4 or SEQ ID NO: 6.
- 15. The oligonucleotide molecule of claim 14 wherein said oligonucleotide molecule comprises a nucleotide sequence complimentary to a nucleotide sequence of 5-50 nucleotides of SEQ ID NO: 4 or SEQ ID NO: 6.
- 16. The oligonucleotide molecule of claim 14 wherein said oligonucleoride molecule comprises a nuclectide sequence complimentary to a nucleotide sequence of 10-40 nucleotides of SEQ ID NO: 4 or SEQ ID NO: 6.
- 17. The oligonucleotide molecule of claim 14 wherein said oligonucleotide molecule comprises a nucleotide sequence complimentary to a nucleotide sequence of 15-25 nucleotides of SEQ ID NO: 4 or SEQ ID NO: 6.
- 18. The oligcnucleotide molecule of claim 14 consisting of a nucleotide sequence complimentary to a nucleotide sequence of at least 10-150 nucleotides of SEQ ID NO: 4 or SEQ ID NO: 6.
- 19. The oligonucleotide molecule of claim 18 consisting of a nucleotide sequence complimentary to a nucleotide sequence of at least 18-28 nucleotides of SEQ ID NO: 4 or SEQ ID NO: 6.
- 20. An isolated antibody which binds to an epitope on SEQ ID NO: 5 and/or SEQ ID NO: 7.
- 21. The antibody of claim 20 wherein said antibody is a monoclonal antibody.
- 22. A method of identifying inhibitors of Mch2α activity comprising the steps of:
performing a test assay by contacting an Mch2α with a substrate in the presence of a test compound under conditions in which said Mch2α processes said substrate in the absence of said test compound, determining whether said substrate is processed.
- 23. A method of identifying activators of Mch2α activity comprising the steps of:
performing a test assay by contacting an Mch2α with a substrate in the presence of a test compound under conditions in which said Mch2α processes said substrate in the absence of said test compound, comparing the rate of processing of the substrate in the presence of the Best compound to the rate of processing the substrate in the absence of the compound.
- 24. A method of inhibiting expression of Mch2 comprising the step of:
contacting cells that express Mch2 with a nucleic acid molecule that comprises oligonucleotide molecule that comprises a nucleotide sequence complimentary to a nucleotide sequence of 5-50 nucleotides of SEQ ID NO: 4 or SEQ ID NO: 6.
ACKNOWLEDGEMENT OF GOVERNMENT RIGHTS
[0001] This invention was made with Government support under Grant A1 35035-01 from the National Institutes of Health. The Government has certain rights in this invention.
Divisions (1)
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Number |
Date |
Country |
Parent |
08896885 |
Jul 1997 |
US |
Child |
09376156 |
Aug 1999 |
US |
Continuations (2)
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Number |
Date |
Country |
Parent |
09376156 |
Aug 1999 |
US |
Child |
10171077 |
Jun 2002 |
US |
Parent |
08446925 |
May 1995 |
US |
Child |
08896885 |
Jul 1997 |
US |