BRIEF DESCRIPTION OF THE DRAWINGS
Further features and advantages of the present invention will become apparent from the following detailed description, taken in combination with the appended drawings, in which:
FIG. 1 is a schematic drawing of an optical oxidative stress measurement device having a 50 μL sample cell with a 1 cm path length supplied with light from a broadband Tungsten Halogen lamp via a first optical fiber, a short wavelength near infrared (SWNIR) spectrophotometer coupled to an opposite end of the sample cell via a second optical fiber for detecting CW intensity in the 600 nm to 1100 nm range, and a computer connected to the spectrophotometer for recording and analyzing the spectra.
FIG. 2 is graph showing absorption levels from a variety of molecular species in the 600 to 1000 nm wavelength range.
FIG. 3 shows a Raman spectra of 20 IVF embryo culture media samples.
FIG. 4 shows mean Raman spectra with Standard Deviation.
FIG. 5 shows spectra for viable samples.
FIG. 6 shows cross validation.
FIG. 7 is a graph of metabolomic signatures of the oxidative stress constituents in the developing embryo obtained using capillary electrophoresis with ultraviolet optical detection which identifies nutrient and metabolite fractions of the media.