Method and apparatus for peptide synthesis and screening

SPECIFICATION
FIELD
This invention relates to methods and apparatus for preparing a non-volatile, reusable, Addressable Synthetic Biopolymer Combinatorial Library (ASBCL) having known sequences at identifiable designated addresses arrayed on a permanent substrate, which library is rapidly creatable by a unique and simple slotted block system. The invention also relates to the use of ASBCLs to screen for sequences having biologic, biochemical, biomedical or therapeutic activity relative to a specified target. The invention permits rapid optimization of leads for identification of active components. When applied to the specific area of peptides. The invention may be termed PILOT, for Peptide Identification and Lead Optimization Technique.
BACKGROUND
Moderate length peptides have attracted considerable research and commercial interest by virtue of the properties some exhibit in enhancing, blocking or otherwise affecting the activity of receptors, microbes, and other molecules deemed biologically significant. Specifically, hexapeptides have proven to have a sufficient chain length to block much larger molecules such as receptors, enzymes and antibodies. Thus, synthetic and natural hexapeptides have exhibited diverse therapeutic properties, among them: Antimicrobials with minimum inhibitory concentrations an order of magnitude less than known natural antimicrobial peptides; bactericides; antivirals; activity as antigenic determinants; and the like. The problem is that there are 64 million (64 m) hexapeptide combinations for the twenty L-amino acids, and another 64 m for the D-amino acids. Indeed if the selection were made from all of the L and D combinations the number amounts to 4.096 billion. Since there are in turn millions of biologically/medically significant targets, preparing a complete suite of just 64 m L-hexapeptides and assaying activity for each of the millions of targets is, practically speaking, an infinite, and therefore, impossible, task.
Accordingly, the Synthetic Peptide Combinational Library (SPCL) approach has recently resulted in a manageable approach to the problem of screening for a unique hexapeptide among the 64 m that is the most active for a given target. In order to be feasible, libraries of large numbers of hexapeptides, on the order of 100,000 or so at a time, must be prepared in quantities sufficient to result in a positively determinable reaction.
There are currently four basic library techniques offered: viral approachs (originated by George Smith of LSU, and by Cetus and Affymax independently); the Houghten approach using Tea-bags; the Selectide bead approach; and the Affymax Chip approach. The latter three have distinct advantages over the viral approach in which peptide libraries are displayed by bacteriophages (viruses that prey on bacteria). A short degenerate oligonucleotide encoding all combinations of a short peptide sequence is cloned into Gene III or XIII of a filamentous phage and expressed on the phage surface. Recombinant phage are screened with the target molecule (e.g. receptor), and phage expressing a certain peptide that binds to the target are identified. Nucleotide sequence analysis of the recombinant Gene III or Gene XIII identifies the peptide sequence displayed by the binding phage.
The problem with the viral approach is that the range of peptides is limited to those tolerable by virus and E. Coli. That is, only a limited suite of peptides can be produced from among the 64 m possible hexapeptides, and likewise for the even greater numbers of longer peptides. Additionally, only L- amino acids are allowed, and each individual hexapeptide of the library is produced within the phage as fusion products. This reduces the flexibility of the sequences, and may mask them entirely.
The Selectide bead approach uses vast quantities of spherical crosslinked polymer beads (Millipore or Cambridge Research Laboratories polyacrylamide beads or Rapp Tentagel polystyrene) divided into 20 equal piles, each of which then has a different L-amino acid coupled to all the beads in the pile. The bead piles are then combined and thoroughly mixed. The resulting single pile is again divided into 20 different piles, each of which is reacted with a different one of the 20 different L-amino acids. This Divide, Couple and Recombine process (DCR) is repeated through six reactions to produce hexapeptides bound to the beads. The beads are then screened against a "target" molecule which is marked with a conjugated enzyme, such as horseradish peroxidase. The target "sticks" to active hexapeptide(s). The bead is rendered visible by adding a substrate for the enzyme which converts it to a colored dye which is precipitated within the beads, and then the visually identified bead(s) are picked out with tweezers. The peptides on the beads are then analyzed, for example by the Edman sequencing method, and soluble versions produced in a synthesizer. The initial screening (locating the target bead(s)) takes only days, the makeup of each identified hexapeptide is unknown, and the analysis and synthesis for confirmation and further work takes much longer.
The Houghten (Iterex) Tea-Bag method, shown in U.S. Pat. No. 4,631,211, employs methylbenzhydrylamine (MBHA) polystyrene beads in a number of foraminous containers, e.g. porous polypropylene bags (Tea-Bags), to prepare a truncated SPCL. In order to shorten the processing time, the Tea-Bag process employs partially known, partially undetermined hexapeptide sequences in repeated screenings, followed by iterative resynthesis to replace the unknown AA sequence positions with known AAs, i.e., A-O.sub.1 O.sub.2 O.sub.3 XXX, A-O.sub.1 O.sub.2 O.sub.3 O.sub.4 XX, etc. The method works on the assumption that a biologically significant response can be detected from a solution which contains hundreds of thousands of inactive components.
The Tea-Bag process typically uses 18 of the 20 L-AAs (cysteine and tryptophane are omitted in the initial library for ease of synthesis), starting with 104,976 combinations of non-determined tetrapeptide resins (XXXX-peptide resins) in 324 aliquots, and adds the 324 known dipeptide sequences (18.sup.2) in the terminal two positions. For epitope determination of antibody binding, the 324 pools are screened to see which best inhibits binding of the target antibody with its natural antigen. The most active amino terminal dipeptide sequences are then incorporated into a further set of 20 pools in which the third residue is varied. These are rescreened for low IC. The most active sequences are again reincorporated iteratively to define positions 4-6 to finally obtain a characterized active hexapeptide.
The Tea-Bags employ MBHA-styrene beads and standard t-Boc chemistry (the conventional Merrifield method) in combination with simultaneous multiple peptide synthesis (SMPS) to prepare the starting 18.sup.4 non-determined XXXX-tetrapeptide library by a DCR process, which assures equimolarity of the peptides on the resin. Briefly, 18 porous polypropylene packets, each containing 4.65 mmol (5.00 g) of MBHA resin, are coupled with each of the protected N-x-t-Boc amino acids. Coupling reactions are checked to ensure they are complete (>99.5%) as assessed by Gisin's picric acid or Kaiser's tests. The resulting resins are then combined and thoroughly mixed as with the Selectide bead process. The resulting resin mixture is separated into 18 portions of equal weight which are placed into porous polypropylene packets, followed by N-a-t-Boc protecting group removal and neutralization of the resulting amine TFA salts. The resin packets are then reacted with solutions of the individual activated amino acids to yield the 324 dipeptide combinations (18.sup.2). The above DCR process is repeated twice more, yielding a final mixture of 104,976 protected tetra-peptide resins (18.sup.4). This XXXX-resin is divided into 324 aliquots (150 mg each) and placed in numbered porous polypropylene packets. Synthesis of the next two defined positions is carried out by SMPS. The peptide mixtures are deprotected and cleaved from their respective resins using low-high hydrogen fluoride (HF) in a multiple HF cleavage apparatus (Multiple Peptide Systems, San Diego, Calif.). Extraction of the individual peptide mixtures was carried out with H.sub.2 O. The competitive ELISA used is a modification of the direct ELISA technique, differing only in the antibody additions step in which 25 microliters each peptide mixture of the SPCL was added with a fixed dilution of the antibody (25 microliters per well).
The foraminous container of the Tea-Bag must retain the solid phase beads, yet have a sufficient number of openings to permit ready entrance and exit of solvent and solute molecules at the reaction temperature, but bar exit of the solid phase. While the synthesis is the standard Merrifield technique, new linking groups that attach the X.sub.n -peptide to the styrene bead supports are disclosed. This process can be characterized as not calling for a continuous support, and it is not addressable.
The Affymax "chip" approach is described in PCT publication W090/10570, which discloses a method for multiple peptide synthesis on a solid support. Photolabilely-blocked amino groups are chemically attached (bonded) to a silicon chip, then irradiated through a patterned mask to selectively remove the blocking groups in a pre-arranged pattern. An amino acid will bond by addition only to the irradiation exposed areas. Additional masks are imposed and radiation applied as a prelude to adding second amino acids. Each amino acid added can include a blocking group so that further addition to that site occurs only after irradiation unblocking. Repeating the process with plural masks builds location specific polypeptides. When the chip is exposed to the target molecule, it may stick to one or more locations. By checking coordinates on a map of the chip, the peptide is identified. However, this process does not work with target molecules stuck to, or part of, cells, and there are exposure problems during processing, i.e., some AA's are light sensitive and cannot be used. Further, the reactions at the surface are not complete; for example, where reaction completion is only 90%, by the 6th iteration to obtain a hexapeptide, only half of them will be made properly.
Accordingly, there is a need in the art for a peptide synthesis and screening process that is rapid and accurately identifies the active peptides from amongst those in an extended, reusable SPCL.
THE INVENTION
OBJECTS
It is among the objects of this invention to provide methods and apparatus for creating a non-volatile, reusable Addressable Synthetic Biopolymer Combinatorial Library (ASBCL) having known amino acid sequences at identifiable designated addresses arrayed on a permanent substrate for rapid screening of target receptors and molecules.
It is another object of the methods and apparatus of this invention to produce ASBCLs in which the biopolymers are peptides, to provide ASPCLs for use in a Peptide Identification and Lead Optimization Technique (PILOT).
It is another object of this invention to provide an improved addressable substrate for adsorption thereon or bonding thereto of biopolymers, such as peptides in known AA sequences, by means of a slotted block system.
It is another object of this invention to provide a simple slotted block system which permits rapid multiple amino acid addition reactions to build peptides of known sequences at identifiable designated addresses in an X-Y coordinate array on a variety of planar substrates.
Still other objects will be evident from the specification, drawings and claims of this application.
DRAWINGS
The invention is disclosed in more detail with reference to the drawings in which:
FIG. 1 is an isometric view of an assembled PILOT slotted block system apparatus, partly broken away to show the various parts in proper alignment and ready for introduction of AA's for reaction with the substrate areas or moieties thereon;
FIGS. 2a and 2b are section views taken in elevation along line 2--2 of FIG. 1 showing two alternative structures of a substrate area in detail;
FIGS. 3A, 3B and 3C illustrate using a combination of two different blocks, one radial and one with concentric annular slots, with a circular support plate to produce a circular array;
FIG. 4 is a section view of the concentric annular block taken along line 4--4 of FIG. 3; and
FIG. 5 is an isometric view of the peripheral frame system of this invention for functionalizing plates prior to condensing biopolymers thereon.
SUMMARY
The invention comprises methods and apparatus for preparing non-volatile, reusable, Addressable SBCLs (ASBCLs) or SPCLs (ASPCLs), having known arrayed dipeptide amino acid sequences incorporated at any desired and known position within any biopolymer, e.g., polypeptide, sequence of length producible by chemical synthesis methodology, in which up to 6 positions (typically 4) may be composed of mixtures of residues, the remaining positions comprising specified amino acids. The arrayed peptides are identifiable from designated addresses provided on a permanent, reusable substrate which permits creation of an ASPCL, typically within a day. While the discussion herein is with reference to hexapeptides by way of example, the principles of the invention are applicable to any binding determinant biopolymer to produce an ASBCL for interaction with any biologically significant target.
The ASBCLs of this invention can uniquely identify the binding determinant biopolymer, e.g. an active hexapeptide, at a unique X-Y coordinate axis upon bonding with or adhering to a fluorescently labeled, radio-labeled or enzyme linked target molecule or receptor, e.g., in solution flowed into contact with the ASPCL-bearing substrate. The amino acids and peptide sequences are substantially equimolar concentrations on the substrate so good quantitative activity is determinable by the fluorescent or radio intensity, or by the optical density of the dye product formed in the enzyme linked probing.
In addition, the hexapeptide array is permanently bound to the plate i.e., is non-volatile, and at each site on the substrate on the order of 1 .mu. mole or more can be bonded. The target molecule can be washed off the substrate plate so that it can be reused repeatedly, particularly for diagnostic testing, as well as rapid active peptide screening on a wide variety of target receptors. For example, a preselected library of peptides, or any other condensation chemistry-based screening agent, may be permanently bonded to a substrate as a diagnostic tool. One example involves exposing an ASPCL plate of this invention to one or more aliquots of a serum which requires diagnosis, and then visualizing binding by passing a flourescently or radiolabeled anti-IGG antibody over the ASPCL plate. Consequently, one or more conditions, such as the presence of antibodies to HIV-1, or the presence of other viral infections can be rapidly and simply diagnosed.
More broadly considered, the peptide may be any biopolymer, thus the terms ASBCL applies to the library on the identifiable designated addresses arrayed on the permanent reusable substrate. It should be understood that the term "substrate" as used herein includes broadly: a) polyolefin plate alone or an activated plate plane substrates; b) a plate with a bead or gel substrate, amino functionalized or or bare, (receptor substrate); c) such beads or gels with spacer arms, amino-functionalized or bare, spacer receptor substrates; and d) reacted substrates i.e. such substrates above with one or more AAs or peptides linked thereto.
The apparatus system of this invention comprises employing an inert substrate support plate, such as a polyolefin polymer, having a plurality of discrete sites (preferably small, well-like, shallow flat-bottomed circular or square depressions) in a spaced array, e.g., 10.times.10, 16.times.16, 20.times.20, 40.times.40, 100.times.100, 400.times.400, or any other desired number. Each of the sites are permanent uniquely addressable locations for assembly of the biopolyer chains or attachment of preactivated molecules. Each site includes an amino functionalized substrate media such as glue-bonded beads or chemically grafted polymeric films, which may be gel-type films. Any conventional peptide substrate, media, or addition chemistry-based agent substrate, may be used.
Examples of bead type substrates are polydimethylacrylamide (PDMA) particles, silica beads, MBHA polystyrene beads, and the like, which are glued to the substrate areas of the support plate. The presently preferred bead substrate is Kieselguhr-encapsulated PDMA particles (Pepsyn-K from Millipore Corp.), secured to a polyethylene plate with a low temperature (<100 degrees C.) hot-melt polyethylene adhesive. The preferred polymeric film is chemically grafted to the surface of the wells by a process disclosed herein, and is particularly useful for screening involving large proteins.
Two methods of attachment of amino-functionalized polymers to form substrate areas on the plates are disclosed by way of examples of the principles of the invention; in situ polymerization (disclosed in detailed examples); and bonding of a pre-polymerized material to activated areas on the plate (disclosed in general). The first polymerizes acryloylated monomers and crosslinking agents onto acryloyl groups attached to the areas of the polyolefin surface (plate activation). This establishes a gel-type polymer covalently grafted into the depressions. This polymer, since it may possess low structural strength, i.e. it need not have high structural strength, can be prepared from monomers at low concentration and with a low molar percentage of cross-linking. The resulting gel substrate materials are therefore highly permeable to proteins, thus greatly improving the sensitivity of detection. The gel film may then be amino functionalized.
A spacer arm derivative is attached to the functional amino groups of the resultant films. These spacer arms, which are also used for the glued beads, increase sensitivity since they reduce unfavorable steric and electronic interactions between the incoming tagged protein and the polymer backbone. An ideal spacer is non-hydrophobic, incapable of forming aggregates by hydrogen bonding, and typically longer than 10A. A variety of materials, including functionalized polyethylene glycols, sugars, and short natural and unnatural peptides may be used as spacers.
The second (pre-polymerized) method of attachment of functional polymers involves attachment of preformed amino-functionalized polymers to the designated areas on the inert support plate (polyolefin). The preformed polymer itself serves as a spacer arm, and access of proteins is improved as compared to the in situ gel type polymers. Examples of preformed amino-functionalized polymers include polyethyleneimine, polyallylamine, long chain functionalized sugars (e.g. dextrans), polyamino acids (e.g. poly-L-lysine) and the like. They can be coupled to acid chloride activated plate areas by reactions of the type described herein. We prefer to use a 500,000 MW dextran that is amino-functionalized pre- or post-attachment to the plate area.
An elastomeric sealing gasket having a plurality of holes therethrough aligned with the substrate areas is placed over the substrate array plate, and a slotted block is placed over the gasketed plate so that individual slots align with rows of substrate areas. Since each area is part of a predetermined array, each defined substrate area has a unique X-Y coordinate address, such as: Row 1, position 1; Row 2, position 20; Row 3, position 78; to Row X.sub.n, position Y.sub.m. Any desired address system may be used, such as sequential numbers for each succeeding area, dual alpha system (AA, AB, AC etc.), or alpha numeric (A1, A2 . . . B1, B2 . . . ).
The slotted block has a height sufficient to provide a well of sufficient volume to receive reaction solution having selected moieties for bonding with the exposed substrate address area or reaction with a previous moiety. Each well can receive a different reactant, e.g. a blocked AA, so that each row has a different A.sub.n position AA. Next, the reactants are removed from the slots in the block, e.g. by decanting or suction, then the amino blocking group removed by a deblocking agent, e.g. piperidine. Then the block is rotated horizontally 90.degree., and each slot well receives another, same or different, reactant so that the A.sub.n+1 position has a predetermined AA. Where the sequence of AAs are the same in each well at each block orientation, turning the block 90.degree. produces all 400 combinations of dipeptides for a 20 slot block/400 substrate area plate system. Iterative application of 3 such plates, two positions being optimized at a time, allows for the identification of the single optimal binding peptide from a 64 m hexapeptide SPCL. The substrate areas can be quite small to provide peptides in adequate (picomolar) amounts.
As an illustration of one method of use of the system of this invention, a random sequence of all XXXX-tetrapeptides on Pepsyn-K beads is prepared, and these beads adhered to the substrate areas. Alternately, a polymeric film gel may be prepared for each area. Then, using the system of this invention, the terminal two AA's can be added thereto in the all-combination 20.times.20 array, and the hexapeptides exposed to targets. A deductive process involving iterative resynthesis of successively smaller libraries can be used to successively characterize the resulting screening-active hexapeptide. Alternately, the procedure and apparatus of this invention can work from a defined middle dipeptide with random ends, followed by replacement of each end in sequence with known dipeptides. Likewise 4 or 5 residues may be mixed, or an array of any kind of peptide, including those including one or more non-natural AAs, can be employed on the reusable substrate of this invention.
The use of beads adhered to the support address area is preferred for diagnostic or drug use applications, as single preselected known-sequence peptide-containing beads can be prepared and glued to the support in a specified address. Likewise, DNA moieties can be bonded to the support, in which case a 16.times.16 array, or an array of 16-4.times.4 subarrays, on a single plate is preferred.
Advantages of the apparatus and methods of this invention include: Synthesis of defined peptides, portions of which optionally can consist of redundant known or unknown (uncharacterized or non-defined sequence) mixtures which are bonded in micromolar amounts in defined arrays with known addresses so that a physical barrier (e.g. an appropriately apertured member) can permit simultaneous screening. Another feature is fluorescent or radio-labeled detection of binding, which provides higher sensitivity and is far more suitable for detection of low affinity interactions than the current Selectide or Iterex technology. The solid array support also permits inference of optional binding elements (e.g. AA sequences) from the spatial position (unique address) rather than requiring chemical determination of sequence.
The ASBCLs and ASPCLs constructed by the method and system of this invention are selectively variable at any two or more positions, while redundant (random selection of all combination) at several other positions (3-6) within peptides or biopolymers of a wide range of size and structure. The system is also useful for screening by itself, or in conjunction with current methods (such as the Iterex Tea-Bag or Selectide methods), for any two or more AA position sequencing, and can be used for progressive refinement of initially identified hits (indications of activity). Because of the effectiveness of the support system of this invention, the separate zones (one or more support address area(s)) can be functionalized for synthesis of peptides at loadings as low as about 0.001 micromoles per cm.sup.2, and typically in the range of from about 0.05 to about 50 .mu. mole/area. The system also permits simultaneous or sequential synthesis by standard Fmoc or t-Boc-chemistry of identified areas (addresses) of distinct known or non-defined peptides, by attaching the slotted block to the substrate plate for simultaneously performing individual separate couplings in the slot compartments. By transforming the block orientation, arrays of peptides may be synthesized at any two positions within a peptide or biopolymer of any length. The previous or following AAs in the peptide sequence may be uniform across the entire substrate surface, and may be unique or consist of mixtures of one or more peptides of known or uncharacterized composition.
While the same block is shown used in different orientations, e.g. rotating a slotted block 90.degree., two dissimilar blocks may be used in the array generating steps, such as a radial slot block (slots extending radially outwardly from a common center) in combination with a block with concentric circular (annular) slots, and the resultant array may be addressed by polar coordinates.
The system of the invention permits displacing the label on the target with a natural ligand to insure specificity of the identification. It also permits reuse of the substrate for repeated probing of the surface by alternative proteins i.e. exposure to different targets followed by washing. Different areas (addresses) may employ the same or different binding materials, e.g. Pepsyn K particles in one area, and grafted films in another.
Although the method and apparatus shown herein are directed to definition of optimal binding linear hexapeptides, it has great applicability in different formats. Especially where the protein of interest, e.g. cytokine receptor, binds a large ligand, then it is advantageous to insert the library within longer sequences, particularly those which are known to form stable secondary structures, as in loops, beta conformations or alpha helices. In the latter, since the library is then displayed on a cylindrical surface, it is of interest to construct the components 3 or 4 residues apart, the components being separated by helix-forming residues, such as alanine. For libraries based on loop structures, either end may be designated as a Cys residue which can then be coupled together by intramolecual disulfide bridges. Cyclic peptides, especially cyclic hexapeptides and cyclic decapeptides can be constructed on PILOT substrate matrices of this invention, and are especially useful for the relative rigidity of these molecules compared to their linear counterparts.
The novel PILOT ASBCL's and methods of this invention provide distinct advantages over the numerous alternatives discussed above in the Background to meet the need for developing new pharmaceutically useful compounds. The specificity of the binding may be uniquely established by side-by-side comparative processing of dual plates which are then probed, one with the presence of the natural ligand, the other without, and the two compared.
One particular advantage of the invention is that it allows detection by numerous methods, but it is unique in being suitable for detection with radiolabelled derivatives, with autoradiographic and counting methods providing the enhanced sensitivity vital for the detection of relatively low-affinity binding peptides which are present in picomolar amounts within pools containing thousands of other non-binding sequences.
Another advantage of the invention is that it allows for the use of standard particulate substrate materials (e.g. Pepsyn K for peptide synthesis, controlled pore glass for DNA/RNA synthesis), for synthesis on the plates, or for preassembly by automated synthesizers followed by arraying these for diagnostic applications. In library applications, the unique method of grafting in-situ generated polymers or attaching preformed polymers to functionalized polyolefin surfaces provides materials far better suited for screening methods than conventional particulate solids. A special virtue is the optical clarity of the film substrates of this invention, combined with their low intrinsic fluorescence which greatly enhances the sensitivity when used with fluorescent tags. Of even greater importance is that the substrate films of this invention are formulated to provide excellent penetration of proteins within their bounds, and side-by-side comparisons with prior art methods have shown significantly enhanced sensitivity with use of this invention.
This invention is unique in being suitable for construction of libraries containing monomer units of almost any kind, for example, bound together by ether, thioether, ester, amine, phosphate, amide or any such bond establishable by organic chemistry methods. Identification is performed solely through spatial recognition, and does not require sequencing, which is generally impossible with other than natural peptide and DNA units.
The PILOT ASBCL's and methods of this invention, are therefore unique, simple, generally applicable and readily duplicated. They provide high sensitivity detection by a variety of tagging procedures.

DETAILED DESCRIPTION OF THE BEST MODE OF THE INVENTION
The following detailed description illustrates the invention by way of example, not by way of limitation of the principles of the invention. This description will clearly enable one skilled in the art to make and use the invention, and describes several embodiments, adaptations, variations, alternatives and uses of the invention, including what we presently believe is the best mode of carrying out the invention.
Referring to FIG. 1 the slotted block system of this invention 1 includes a base 2 which has or receives orienting members, such as a plurality of guide and securing rods 3 with wing nuts 4. The base 2 receives a substrate support plate 5 which includes an array of areas 6. As best seen in FIG. 2a, each area 6 includes substrate 7 (in this case beads) secured in depression 8 by a suitable glue 9. FIG. 2b shows a grafted polymeric film as the substrate 7 in depression 8. Overlying the substrate support plate 5 is a gasket 10, preferably a sheet of chemically inert elastomeric material (e..g. Viton or silicon rubber), having an array of holes 11 therethrough which are in alignment with the substrate areas 6. The gasket functions to prevent leakage between individual substrate areas 6. A slotted block 12, having holes 13 therethrough to receive the guide/securing rods 3 is placed over the gasket 10. This block 12 includes a plurality of slots 13, 14, 15 etc. therein, which are aligned with and extend a full row width of the substrate areas 6. The slots may be identified, as by the alpha designations A,B,C etc. shown. Note in the substrate support plate 5, additional corresponding rows L,M,N . . . are shown. In a typical block 12, there will be some 10 to 400 such slots. Fastening the wingnuts 4 secures the assembly together in the proper orientation and prevents leakage between adjacent slots and substrate areas when individual reaction solutions are placed in the wells formed by the slots. After reaction and removal of solution, upon rotating the block 12 by 90.degree. and reintroducing solutions in the slots, a known array of dipeptide sequences is produced.
FIGS. 3A, 3B and 3C show an important variation involving a round substrate support plate 20 having a concentric/radial array 21 of discrete substrate areas 6. A gasket 22 also includes a corresponding concentric/radial array of holes 23. In this embodiment, two slotted plates may be employed sequentially: slotted radial plate 24, and concentric slotted plate 25, in either sequence, 24, 25 or 25, 24. There may be fewer radial slots 26 than the radial array of holes 23 or substrate areas 21, in which case the block 24 may be turned between application of reactants (e.g., AA solutions). The inner concentric segments 27, 28, 29 etc. may be secured in spaced relationship by horizontal rods 30, 31, which are spaced above the bottom 32 of block 25 to insure access of solution to all relevant substrate areas. In FIG. 2 the base plate and pins are omitted for clarity.
FIG. 4 shows in vertical cross section the construction of the concentric slotted plate 25 in which outer ring 33 is spaced from inner core 34 by rod 31. A series of tubular spacers 35, 36, 37, 38 on rod 31 space the concentric intermediate rings 27, 28, 29 to provide concentric annular slots. The rod 31 may be countersunk in bore 39.
FIG. 5 shows a border frame assembly for functionalizing the substrate areas 8 on support plate 5, e.g., with common AAs, or mixtures of AAs, and for deblocking, washing, probing (screening) and addition of spacer arm derivatives. An edge gasket 40 is placed on the substrate plate 5, then a border frame 41 is placed thereover and secured with clamps 42, 43. This provides a central well 45 for the entire array for receiving the appropriate solutions for the functionalizing chemistry.
The following example protocols describe the sequential steps of the method:
EXAMPLE 1
Determination of AA Mixture Proportions for Equimolar Incorporation on Substrate Beads:
The component amino acid (Fmoc-X-OH,1 equivalent) is mixed with Fmoc-Nle-OH (1 equivalent), then dissolved and activated by the addition of PyBOP, HOBt and NMM solutions. After 10 minutes the mixture is added to Nva-PAL-Pepsyn K support (5 mg). After 2 hours the support is washed with DMF repeatedly, treated with 30% piperidine in DMF (to remove incorporated Fmoc- groups), washed with DMF, and methanol, then treated with TFA/water (95:5) for 2 hours. The TFA solution is expelled into a vial, a known proportion of it dried down in vacuo, and the resultant mixed dipeptides X-Nva and Nle-Nva are hydrolysed with 6M HCl at 150 degrees for 1 hour. The relative incorporations of X and Nva are then determined by amino acid analysis. A graph is constructed plotting molar % X (in this initial round X is 50%) against molar percentage incorporated; and the curve which results when using extremities points at 0 and 100% is used to predict what molar percent X would give equal incorporation to that of Nle.
The process is repeated using the predicted molar percent X to confirm and, if necessary, iteratively refine the molar percent. This method has been applied to all 20 natural L-amino acids, most D-amino acids, and several unnatural amino acids, such as beta-alanine and 2-napthylalanine.
For any desired library mixture, the amino acids are selected, mixed in the correct ratios, activated, coupled to the support, and the equal incorporation confirmed by analysis. For the 10 amino acid library the subject of these examples the following recipe gives equal incorporation: Fmoc-L-Nle-OH 0.188 g; Fmoc-L-His(Trt)-OH 0.73 g; Fmoc-L-Pro-OH 0.24 g; Fmoc-L-Gln(Trt)-OH 0.797 g; Fmoc-L-Tyr(tBu)-OH 0.398 g; Fmoc-Gly-OH 0.093 g; Fmoc-L-Phe-OH 0.206 g; Fmoc-L-Arg(Pmc)-OH 1.25 g; Fmoc-L-Glu(OtBu)-OH 0.288 g; and Fmoc-D-Ala-OH 0.130 g. To these mixed amino acids were added HOBt 1.614 g, and the entire solids totally dissolved in DMF and made up to a volume of 40 mL. For coupling, 10 mL of this solution, called MIX solution, is added to 1.71 g PyBOP reagent, mixed, 0.35 mL of N-methylmorpholine is added, remixed and left for 10 minutes. This solution is adequate to completely cover and react a single 10.times.10 plate giving equal incorporation.
EXAMPLE 2
Preparation of A "Beaded" Plate (Support Plate With Array of Bead-Type Substrate Areas):
The sequence BAla-BAla-BAla-Nle-BAla-Nle-BAla-BAla was assembled on 0.2 mmol/g Pepsyn-K (Millipore) functionalized by treatment with ethylenediamine using a Milligen/Biosearch model 9600 peptide synthesizer using standard BOP+HOBt coupling protocols. Ace Hardware Hot Melt adhesive was cut into thin sections and melted at as low a temperature as possible on a flat PTFE sheet to produce a thin sheet (in a range of from about 0.2 to about 1.0 mm thick) of hot melt adhesive (HMA sheet). The PTFE sheet was removed from the heat, and dry, peptide-bearing Pepsyn-K beads were sprinkled over the melted glue surface and gently patted down. After several hours of cooling, excess beads were removed and the glue sheet lifted off the PTFE sheet, then punched into appropriate circles (e.g. 1-10 mm dia) with a standard hole punch. The resultant discs were then attached to an array of shallow, dished wells in a polyethylene sheet using a Black and Decker Thermogrip glue gun adding a dab of glue in the well and pushing the discs down firmly. The discs can be reinforced with polypropylene or metal mesh. Typically, each patch bears 5 mg of beads, having 1 micromole of spacer arm linked Pepsyn-K. The top right hand corner of every plate is notched or drilled as a reference to prevent the plate being incorrectly aligned at any step.
EXAMPLE 3
A Preparation of A Polymeric "Film" Plate:
A 10 mm thick plate of linear high density polyethylene is floated in a water bath at 70.degree. C. and treated with 5M chromium trioxide in 5.3M sulfuric acid for two hours. The plate is washed with water many times, then with methanol, and then with ether, and dried under vacuum. The surface bound carboxylic acids are converted to acid chlorides by treatment with 20% thionyl chloride in chloroform for two hours. The plate is rapidly washed with chloroform, then ether, and dried under a stream of nitrogen and used immediately. This acid chloride functionalized plate can be derivatized by a variety of reagents to introduce many functionalities. Linear polymers, such as polyethylenimine, poly(amino)functionalized polyethylene glycols, and saccharide may be added to the plates by conventional chemistries. For example, for introduction of acryloyl groups, the plate may be treated with either a solution of N-(3-aminopropyl) methacrylamide hydrochloride and triethylamine in DMF, or it may be treated with neat (undiluted) diaminopropane for two hours, followed by washing thoroughly with water, then methanol, then ether, and then treated with a solution of acryloyl chloride and disopropylethyl amine in THF. The plate is washed well consecutively with methanol and ether, and dried under vacuum. The plate is now ready for grafting or casting of a gel film thereon as the substrate in the specific address areas.
The gels which may be cast into the wells of the substrate plate or grafted to the acryloyl groups on the surface of a film plate can have a variety of concentrations, cross-linking levels, functional linkers and amino linker loading. To prepare a typical gel, under nitrogen, a 5 ml portion of deoxygenated water (under vacuum for 20 minutes) is added to 18.5 mg of bisacryloyldiaminohexane, 295 mg of dimethylacrylamide, and 186 mg of the monoacrylamide of 1,6 diaminohexane hydrochloride. This is filtered onto 15 mg of ammonium persulfate and treated with 30 uL of pH 6 TEMED solution in water. In a glove bag under nitrogen, the monomer solution is rapidly transferred to each well of the plate. The plate is sealed in a plastic bag with an open beaker of deoxygenated water and allowed to gel. After curing over night the plate is washed with water and soaked in 1N sodium hydroxide for 2 hours. Two water washes of 15 minutes each followed by at least four washes in DMF give a plate which is ready for peptide synthesis.
EXAMPLE 4
Spacer Arm Derivatization of The Film:
Optionally a gel film plate of the type in Example 3 may have a tetrapeptide spacer attached to the substrate. A plate prepared as in Example 3 had Fmoc-beta-alanine (BAla) coupled to it (standard PyBOP+HOBt/NMM procedure, 2 hours). Following thorough DMF washes, the plate was treated with 30% piperidine in DMF (1, 45 minutes). The plate was washed 2 times with DMF, and the piperidine treatments and subsequent washes were pooled and read spectrophotometrically at 301 nm to determine the Fmoc-loading (in this example 2 micromoles per substrate area). Three more coupling cycles were then performed adding Fmoc-epsilon-aminocaproic acid twice, then beta-alanine again to give the final BAla-Aca-BAla spacer arm film plate as used in the preferred embodiment for ASBCL or ASPCL libraries.
EXAMPLE 5
Construction of A 10.times.10 Hexapeptide ASPCL Plate:
The method of this invention allows the construction of arrays of sequences at any 2 sequence positions within peptides of any reasonable size with several positions being incorporated as mixtures. The preferred embodiment of the method is to prepare hexapeptides with the central 2 AA's arrayed, the other 4 positions redundantly mixed, and the final sequence AA is N-acetylated. For film plates it is preferred to add a spacer arm peptide to the film prior to construction of the library, and with beaded plates an octapeptide spacer is attached prior to embedding in the glue. The following sequence of operations is followed to prepare a Hexapeptide ASPCL with a known central (3,4) AA sequence:
i) Apply an edge gasket and a border-frame spacer on the substrate plate (see FIG. 5) to make a flat "dish" type reactor. As an alternative to use of the screwed pin base plate assembly of FIG. 1, one may use standard large office clips, or wing nuts and standard bolts and washers to hold the parts together;
ii) Wash with DMF 2.times. using horizontal action shaker;
iii) Couple 10 mL preactivated MIX solution made as per Example 1 for 2 hours, while covering plate with foil tent;
iv) Wash with DMF 3.times.;
v) Deblock with 30% piperidine in DMF 1 min, 10 min;
vi) Wash with DMF 5.times.;
vii) Repeat steps iii) to vi);
viii) Dismount edge gasket and frame spacer, and mount slotted block assembly with 100 hole gasket to base plate as in FIG. 1 with slots in a first, horizontal orientation (L TO R when facing the assembly). DMF solvent is placed in alternate wells and the dry wells observed carefully to ensure no leakage. Prepare in vials the following amino acids: 1. Fmoc-L-Nle-OH 0.14 g; 2. Fmoc-L-His(Trt)-OH 0.25 g; 3. Fmoc-L-Pro-OH 0.135 g; 4. Fmoc-L-Gln(Trt)-OH 0.244 g; 5. Fmoc-Tyr(tBu)OH 0.183 g, 6. Fmoc-Gly-OH 0.116 g; 7. Fmoc-L-Phe-OH 0.154 g; 8. Fmoc-L-Arg(Pmc)-OH 0.265 g; 9. Fmoc-L-Glu(OtBu)-OH 0.17 g; 10. Fmoc-D-Ala-OH 0.124 g. To each of these vials add and mix PyBOP 0.27 g and HOBt 0.06 g, and 2 mL of 0.3M N-methylmorpholine in DMF. Add each to a designated horizontal slot: 1 to top slot; 2 to next slot, etc.;
ix) Maintain at room temperature for 2 hours to complete coupling;
x) Disassemble and remount with edge-gasket and border-frame spacer;
xi) Wash with DMF 3.times.;
xii) Deprotect with 30% piperidine in DMF 1 minute, 10 minutes;
xiii) Wash with DMF 5.times.;
xiv) Mount slot block with slots rotated 90.degree., i.e., in a vertical orientation, and repeat coupling as described in viii) except 1 is coupled to the left hand slot, 2 to the next slot, etc.;
xv) Disassemble and remount with edge-gasket and border-frame spacer;
xvi) Remove Fmoc group and perform 2 cycles of mixture incorporation as described in i) to vii);
xvii) Remove Fmoc-group and wash with DMF 5.times.;
xviii) Acetylate with 0.3M acetic anhydride +0.3M HOBt in DMF (10 mL) for 1 hour;
xix) Wash DMF 5.times., Methanol 5.times.;
xx) Treat with 95:5 TFA/water for 2 hours;
xxi) Wash Methanol 5.times., aqueous buffer 5.times.. Store in sealed bag at 4 degrees prior to probing to screen a target.
Numbering from the carboxy terminus, the resulting hexapeptides are characterized as XX-A.sub.4, A.sub.3 -XX with the A.sub.3 and A.sub.4 known sequence being uniquely addressable. That is, the central dipeptide is known from its unique address by use of the slotted block, the hexapeptide at address 001 being XX-D-Ala-L-Nle-XX, at address 002 being XX-L-Glu-L-Nle-XX, etc., to address 100 being XX-L-Nle-D-Ala-XX.
EXAMPLE 6
Construction of A 10.times.10 Hexapeptide ASPCL on a Gel Film Plate
Instead of a bead plate, a gel film plate as in Examples 3 and 4 may be similarly employed to construct an ASPCL library by the process of Example 5. This gel film ASPCL is used to screen, see Example 6 below.
EXAMPLE 7
Determination of Binding Elements in the Interaction of Streptavidin with Peptides
A film library plate of Examples 3 and 4 was constructed similarly to Example 6 with the selection of 10 amino acids as indicated. .sup.125 I labelled streptavidin was prepared and purified by standard procedures; a fluorescently labelled form was also prepared by reaction of AMCA-NHS (Pierce) with the protein, excess reagent being removed by dialysis. Firstly, the iodinated protein was incubated with the plate overnight in a phosphate buffer containing 150 mM salt, Tween detergent and bovine serum albumin (1 mg/mL). The plate was washed with the buffer 3 times, placed on a standard laboratory X-ray film with an enhancer plate and exposed overnight. The developed film shows strong affinity in specific address locations corresponding to 2 central dipeptides HP and RR. The plate was then washed repeatedly with 6M guanidine hydrochloride, and buffer medium, then reincubated with the fluorescent AMCA-streptavidin overnight. After washing the plate was irradiated with long wave length uv light and visible confirmation obtained of the previously deduced binding elements. Subsequent iterations as described above further defines the active dipeptides at each end for complete hexapeptide characterization.
It should be understood that various modifications within the scope of this invention can be made by one of ordinary skill in the art without departing from the spirit thereof. We therefore wish our invention to be defined by the scope of the appended claims in view of the specification as broadly as the prior art will permit.
__________________________________________________________________________SEQUENCE LISTING(1) GENERAL INFORMATION:(iii) NUMBER OF SEQUENCES: 101(2) INFORMATION FOR SEQ ID NO: 1:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Addresses 1.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 1: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 1:XaaXaaLeuAlaXaaXaa15(2) INFORMATION FOR SEQ ID NO: 2:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 2.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 2: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 2:XaaXaaLeuGluXaaXaa15(2) INFORMATION FOR SEQ ID NO: 3:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 3.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 3: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 3:XaaXaaLeuArgXaaXaa15(2) INFORMATION FOR SEQ ID NO: 4:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 4.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 4: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 4:XaaXaaLeuPheXaaXaa15(2) INFORMATION FOR SEQ ID NO: 5:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 5.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 5: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 5:XaaXaaLeuGlyXaaXaa15(2) INFORMATION FOR SEQ ID NO: 6:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 6.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 6: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 6:XaaXaaLeuTyrXaaXaa15(2) INFORMATION FOR SEQ ID NO: 7:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 7.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 7: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 7:XaaXaaLeuGlnXaaXaa15(2) INFORMATION FOR SEQ ID NO: 8:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 8.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 8: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 8:XaaXaaLeuProXaaXaa15(2) INFORMATION FOR SEQ ID NO: 9:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 9.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 9: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 9:XaaXaaLeuHisXaaXaa15(2) INFORMATION FOR SEQ ID NO: 10:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 10.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 10: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 10:XaaXaaLeuLeuXaaXaa15(2) INFORMATION FOR SEQ ID NO: 11:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 11.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 11: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 11:XaaXaaHisAlaXaaXaa15(2) INFORMATION FOR SEQ ID NO: 12:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 12.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 12: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 12:XaaXaaHisGluXaaXaa15(2) INFORMATION FOR SEQ ID NO: 13:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 13.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 13: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 13:XaaXaaHisArgXaaXaa15(2) INFORMATION FOR SEQ ID NO: 14:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 14.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 14: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 14:XaaXaaHisPheXaaXaa15(2) INFORMATION FOR SEQ ID NO: 15:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 15.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 15: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 15:XaaXaaHisGlyXaaXaa15(2) INFORMATION FOR SEQ ID NO: 16:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 16.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 16: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 16:XaaXaaHisTyrXaaXaa15(2) INFORMATION FOR SEQ ID NO: 17:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 17.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 17: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 17:XaaXaaHisGlnXaaXaa15(2) INFORMATION FOR SEQ ID NO: 18:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 18.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 18: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 18:XaaXaaHisProXaaXaa15(2) INFORMATION FOR SEQ ID NO: 19:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 19.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 19: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 19:XaaXaaHisHisXaaXaa15(2) INFORMATION FOR SEQ ID NO: 20:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 20.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 20: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 20:XaaXaaHisLeuXaaXaa15(2) INFORMATION FOR SEQ ID NO: 21:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 21.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 21: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 21:XaaXaaProAlaXaaXaa15(2) INFORMATION FOR SEQ ID NO: 22:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 22.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 22: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 22:XaaXaaProGluXaaXaa15(2) INFORMATION FOR SEQ ID NO: 23:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 23.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 23: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 23:XaaXaaProArgXaaXaa15(2) INFORMATION FOR SEQ ID NO: 24:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 24.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 24: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 24:XaaXaaProPheXaaXaa15(2) INFORMATION FOR SEQ ID NO: 25:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 25.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 25: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 25:XaaXaaProGlyXaaXaa15(2) INFORMATION FOR SEQ ID NO: 26:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 26.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 26: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 26:XaaXaaProTyrXaaXaa15(2) INFORMATION FOR SEQ ID NO: 27:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 27.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 27: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 27:XaaXaaProGlnXaaXaa15(2) INFORMATION FOR SEQ ID NO: 28:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 28.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 28: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 28:XaaXaaProProXaaXaa15(2) INFORMATION FOR SEQ ID NO: 29:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 29.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Exhibits specific bindingactivity with streptavidin. Xaa represents arandom amino acid selected from mixtures of thefollowing 10 amino acids to give equalincorporation: Nle, His, Pro, Gln, Tyr, Gly, Phe,Arg, Glu, Ala. Where Leu or Ala is specified atany position in the sequence, then Leu refers toNle (or normal-leucine) and Ala refers to Ala (orbeta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 29: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 29:XaaXaaProHisXaaXaa15(2) INFORMATION FOR SEQ ID NO: 30:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 30.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 30: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 30:XaaXaaProLeuXaaXaa15(2) INFORMATION FOR SEQ ID NO: 31:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 31.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 31: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 31:XaaXaaGlnAlaXaaXaa15(2) INFORMATION FOR SEQ ID NO: 32:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 32.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 32: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 32:XaaXaaGlnGluXaaXaa15(2) INFORMATION FOR SEQ ID NO: 33:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 33.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 33: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 33:XaaXaaGlnArgXaaXaa15(2) INFORMATION FOR SEQ ID NO: 34:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 34.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 34: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 34:XaaXaaGlnPheXaaXaa15(2) INFORMATION FOR SEQ ID NO: 35:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 35.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 35: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 35:XaaXaaGlnGlyXaaXaa15(2) INFORMATION FOR SEQ ID NO: 36:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 36.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 36: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 36:XaaXaaGlnTyrXaaXaa15(2) INFORMATION FOR SEQ ID NO: 37:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 37.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 37: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 37:XaaXaaGlnGlnXaaXaa15(2) INFORMATION FOR SEQ ID NO: 38:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 38.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 38: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 38:XaaXaaGlnProXaaXaa15(2) INFORMATION FOR SEQ ID NO: 39:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 39.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 39: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 39:XaaXaaGlnHisXaaXaa15(2) INFORMATION FOR SEQ ID NO: 40:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 40.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 40: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 40:XaaXaaGlnLeuXaaXaa15(2) INFORMATION FOR SEQ ID NO: 41:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 41.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 41: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 41:XaaXaaTyrAlaXaaXaa15(2) INFORMATION FOR SEQ ID NO: 42:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 42.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 42: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 42:XaaXaaTyrGluXaaXaa15(2) INFORMATION FOR SEQ ID NO: 43:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 43.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 43: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 43:XaaXaaTyrArgXaaXaa15(2) INFORMATION FOR SEQ ID NO: 44:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 44.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 44: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 44:XaaXaaTyrPheXaaXaa15(2) INFORMATION FOR SEQ ID NO: 45:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 45.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 45: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 45:XaaXaaTyrGlyXaaXaa15(2) INFORMATION FOR SEQ ID NO: 46:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 46.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 46: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 46:XaaXaaTyrTyrXaaXaa15(2) INFORMATION FOR SEQ ID NO: 47:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 47.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 47: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 47:XaaXaaTyrGlnXaaXaa15(2) INFORMATION FOR SEQ ID NO: 48:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 48.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 48: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 48:XaaXaaTyrProXaaXaa15(2) INFORMATION FOR SEQ ID NO: 49:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 49.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 49: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 49:XaaXaaTyrHisXaaXaa15(2) INFORMATION FOR SEQ ID NO: 50:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 50.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 50: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 50:XaaXaaTyrLeuXaaXaa15(2) INFORMATION FOR SEQ ID NO: 51:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 51.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 51: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 51:XaaXaaGlyAlaXaaXaa15(2) INFORMATION FOR SEQ ID NO: 52:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 52.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 52: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 52:XaaXaaGlyGluXaaXaa15(2) INFORMATION FOR SEQ ID NO: 53:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 53.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 53: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 53:XaaXaaGlyArgXaaXaa15(2) INFORMATION FOR SEQ ID NO: 54:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 54.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 54: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 54:XaaXaaGlyPheXaaXaa15(2) INFORMATION FOR SEQ ID NO: 55:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 55.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 55: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 55:XaaXaaGlyGlyXaaXaa15(2) INFORMATION FOR SEQ ID NO: 56:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 56.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 56: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 56:XaaXaaGlyTyrXaaXaa15(2) INFORMATION FOR SEQ ID NO: 57:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 57.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 57: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 57:XaaXaaGlyGlnXaaXaa15(2) INFORMATION FOR SEQ ID NO: 58:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 58.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 58: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 58:XaaXaaGlyProXaaXaa15(2) INFORMATION FOR SEQ ID NO: 59:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 59.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 59: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 59:XaaXaaGlyHisXaaXaa15(2) INFORMATION FOR SEQ ID NO: 60:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 60.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 60: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 60:XaaXaaGlyLeuXaaXaa15(2) INFORMATION FOR SEQ ID NO: 61:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 61.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 61: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 61:XaaXaaPheAlaXaaXaa15(2) INFORMATION FOR SEQ ID NO: 62:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 62.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 62: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 62:XaaXaaPheGluXaaXaa15(2) INFORMATION FOR SEQ ID NO: 63:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 63.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 63: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 63:XaaXaaPheArgXaaXaa15(2) INFORMATION FOR SEQ ID NO: 64:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 64.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 64: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 64:XaaXaaPhePheXaaXaa15(2) INFORMATION FOR SEQ ID NO: 65:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 65.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 65: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 65:XaaXaaPheGlyXaaXaa15(2) INFORMATION FOR SEQ ID NO: 66:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 66.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 66: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 66:XaaXaaPheTyrXaaXaa15(2) INFORMATION FOR SEQ ID NO: 67:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 67.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 67: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 67:XaaXaaPheGlnXaaXaa15(2) INFORMATION FOR SEQ ID NO: 68:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 68.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 68: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 68:XaaXaaPheProXaaXaa15(2) INFORMATION FOR SEQ ID NO: 69:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 69.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 69: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 69:XaaXaaPheHisXaaXaa15(2) INFORMATION FOR SEQ ID NO: 70:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 70.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 70: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 70:XaaXaaPheLeuXaaXaa15(2) INFORMATION FOR SEQ ID NO: 71:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 71.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 71: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 71:XaaXaaArgAlaXaaXaa15(2) INFORMATION FOR SEQ ID NO: 72:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 72.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 72: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 72:XaaXaaArgGluXaaXaa15(2) INFORMATION FOR SEQ ID NO: 73:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 73.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Exhibits specific bindingactivity with Streptavidin. Xaa represents arandom amino acid selected from mixtures of thefollowing 10 amino acids to give equalincorporation: Nle, His, Pro, Gln, Tyr, Gly, Phe,Arg, Glu, Ala. Where Leu or Ala is specified atany position in the sequence, then Leu refers toNle (or normal-leucine) and Ala refers to Ala (orbeta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 73: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 73:XaaXaaArgArgXaaXaa15(2) INFORMATION FOR SEQ ID NO: 74:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 74.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 74: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 74:XaaXaaArgPheXaaXaa15(2) INFORMATION FOR SEQ ID NO: 75:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 75.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 75: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 75:XaaXaaArgGlyXaaXaa15(2) INFORMATION FOR SEQ ID NO: 76:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 76.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 76: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 76:XaaXaaArgTyrXaaXaa15(2) INFORMATION FOR SEQ ID NO: 77:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 77.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 77: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 77:XaaXaaArgGlnXaaXaa15(2) INFORMATION FOR SEQ ID NO: 78:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 78.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 78: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 78:XaaXaaArgProXaaXaa15(2) INFORMATION FOR SEQ ID NO: 79:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 79.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 79: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 79:XaaXaaArgHisXaaXaa15(2) INFORMATION FOR SEQ ID NO: 80:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 80.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 80: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 80:XaaXaaArgLeuXaaXaa15(2) INFORMATION FOR SEQ ID NO: 81:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 81.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 81: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 81:XaaXaaGluAlaXaaXaa15(2) INFORMATION FOR SEQ ID NO: 82:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 82.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 82: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 82:XaaXaaGluGluXaaXaa15(2) INFORMATION FOR SEQ ID NO: 83:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 83.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 83: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 83:XaaXaaGluArgXaaXaa15(2) INFORMATION FOR SEQ ID NO: 84:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 84.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 84: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 84:XaaXaaGluPheXaaXaa15(2) INFORMATION FOR SEQ ID NO: 85:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 85.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 85: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 85:XaaXaaGluGlyXaaXaa15(2) INFORMATION FOR SEQ ID NO: 86:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 86.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 86: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 86:XaaXaaGluTyrXaaXaa15(2) INFORMATION FOR SEQ ID NO: 87:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 87.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 87: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 87:XaaXaaGluGlnXaaXaa15(2) INFORMATION FOR SEQ ID NO: 88:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 88.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 88: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 88:XaaXaaGluProXaaXaa15(2) INFORMATION FOR SEQ ID NO: 89:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 89.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 89: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 89:XaaXaaGluHisXaaXaa15(2) INFORMATION FOR SEQ ID NO: 90:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 90.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 90: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 90:XaaXaaGluLeuXaaXaa15(2) INFORMATION FOR SEQ ID NO: 91:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 91.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 91: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 91:XaaXaaAlaAlaXaaXaa15(2) INFORMATION FOR SEQ ID NO: 92:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 92.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 92: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 92:XaaXaaAlaGluXaaXaa15(2) INFORMATION FOR SEQ ID NO: 93:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 93.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 93: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 93:XaaXaaAlaArgXaaXaa15(2) INFORMATION FOR SEQ ID NO: 94:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 94.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 94: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 94:XaaXaaAlaPheXaaXaa15(2) INFORMATION FOR SEQ ID NO: 95:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 95.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 95: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 95:XaaXaaAlaGlyXaaXaa15(2) INFORMATION FOR SEQ ID NO: 96:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 96.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 96: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 96:XaaXaaAlaTyrXaaXaa15(2) INFORMATION FOR SEQ ID NO: 97:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 97.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 97: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 97:XaaXaaAlaGlnXaaXaa15(2) INFORMATION FOR SEQ ID NO: 98:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 98.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 98: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 98:XaaXaaAlaProXaaXaa15(2) INFORMATION FOR SEQ ID NO: 99:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 99.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 99: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 99:XaaXaaAlaHisXaaXaa15(2) INFORMATION FOR SEQ ID NO: 100:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 6(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: 10 .times. 10 Array Sequence Ligands forStreptavidin Test, Address 100.(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using anArris Pharmaceutical PILOT Support Array System(D) OTHER INFORMATION: Biological activity unknown.Xaa represents a random amino acid selected frommixtures of the following 10 amino acids to giveequal incorporation: Nle, His, Pro, Gln, Tyr, Gly,Phe, Arg, Glu, Ala. Where Leu or Ala isspecified at any position in the sequence, then Leurefers to Nle (or normal-leucine) and Ala refers toAla (or beta- alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 100: 1 to 6(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 100:XaaXaaAlaLeuXaaXaa15(2) INFORMATION FOR SEQ ID NO: 101:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 8(B) TYPE: Amino Acids(C) STRANDEDNESS:(D) TOPOLOGY: Linear(ii) MOLECULE TYPE: Peptide(A) DESCRIPTION:(ix) FEATURE:(A) NAME/KEY: Pepsyn-K Bead Test Peptide(B) LOCATION:(C) IDENTIFICATION METHOD: Constructed using aMilligen/Biosearch Model 9600 peptide synthesizer.(D) OTHER INFORMATION: Biological activity notdetermined. Xaa represents a random amino acidselected from mixtures of the following 10 aminoacids to give equal incorporation: Nle, His, Pro,Gln, Tyr, Gly, Phe, Arg, Glu, Ala. Where Leu orAla is specified at any position in the sequence,then Leu refers to Nle (or normal-leucine) and Alarefers to Ala (or beta-alanine).(x) PUBLICATION INFORMATION:(A) AUTHORS:(B) TITLE:(C) JOURNAL:(D) VOLUME:(E) ISSUE:(F) PAGES:(G) DATE:(H) DOCUMENT NUMBER:(I) FILING DATE:(J) PUBLICATION DATE:(K) RELEVANT RESIDUES IN SEQ ID NO: 101: 1 to 8(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 101:AlaAlaAlaLeuAlaLeuAlaAla15__________________________________________________________________________

Number	Name	Date
4631211	Houghten	Dec 1986
4728502	Hamill	Mar 1988
5010175	Rutter et al.	Apr 1991
5100626	Levin	Mar 1992
5143854	Pirrung et al.	Sep 1992
5188733	Wang et al.	Feb 1993

Number	Date	Country
8600991	Feb 1986	WOX
WO9015070	Dec 1990	WOX

Method and apparatus for peptide synthesis and screening

Information

Patent Number

Date Filed

Date Issued

Inventors

Original Assignees

Examiners

Agents

CPC

US Classifications

Field of Search

US

International Classifications

Abstract

Description

Claims

US Referenced Citations (6)

Foreign Referenced Citations (2)

Non-Patent Literature Citations (5)

Entry
Frank et al., "Facile and rapid `spot-synthesis` of large numbers of peptides on membrane sheets", pp. 151-152 in Peptides 1990, ed. by Giralt et al., (1991) ESCOM Science Publishers B.V.
Wang et al., Peptide Research, vol. 5, No. 5, pp. 275-280, (Sep.-Oct. 1992) "Multiple Peptide Synthesis on Polypropylene Membranes for Rapid Screening of Bioactive Peptides".
Light-Directed, Spatially Addressable Parallel Chemical Synthesis by Stephen P. A. Fodor, J. Leighton Read, Michael C. Pirrung, Lubert Stryer, Amy Tsai Lu, and Dennis Solas, Research Article, Feb. 15, 1991, p. 767.
Generation and Use of Synthetic peptide Combinatorial Libraries for Basic Research and Drug Discovery by Richard A. Houghten, Clemencia Pinilla, Sylvie E. Blondelle, Jon R. Appel, Colette T. Dooley and Julio H. Cuervo, Nature, vol. 354, Nov. 7, 1992, p. 84.
Science and Technology--The Silver Shotguns, The Economist, Dec. 14, 1991 p. 119.