Claims
- 1. A method for immunochemical measurement of an antigen or antibody which comprises labelling an antigen or antibody with a labelling substance,
- said labelling substance being a cyanine or merocyanine dye comprising a carboxy-containing substituent on a heterocyclic nucleus of said dye, wherein said cyanine dye comprises a carboxy-containing substituent bound to an atom other than the nitrogen atom in the cyanine color-forming moiety of the terminal heterocyclic nucleus of said cyanine dye and wherein said merocyanine dye comprises a carboxy-containing substituent on the heterocyclic nucleus of said merocyanine dye.
- 2. A method for immunochemically measuring an antigen or antibody which comprises:
- labelling an antigen or antibody with a cyanine or merocyanine dye comprising a carboxy-containing substituent on a heterocyclic nucleus of said dye, wherein said cyanine dye comprises a carboxy-containing substituent bound to an atom other than the nitrogen atom in the cyanine color-forming moiety of the terminal heterocyclic nucleus of said cyanine dye and wherein said merocyanine dye comprises a carboxy-containing substituent on the heterocyclic nucleus of said merocyanine dye,
- subjecting the thus obtained dye-labelled antigen or antibody to an immune reaction,
- bringing either the bound antibody or antigen or the free antigen or antibody into contact with silver halide after or at the same time as B/F separation,
- exposing the resulting product to light which the corresponding dye absorbs,
- developing the exposed silver halide, and,
- measuring the resulting optical density.
- 3. The method of claim 2 wherein said cyanine dye comprises a carboxy-containing substituent bound to an atom other than the nitrogen atom in the cyanine-color-forming moiety of the terminal heterocyclic nucleus of said dye.
- 4. The method of claim 1 or 2 wherein said cyanine dye has the formula: ##STR8## wherein ma and na each represents 1 or 2 and may be the same or different; pa represents 2 or 3; qa represents 1 or 2; L.sub.1, L.sub.2 and L.sub.3, which each may be the same or different represents a methine group optionally substituted with a lower alkyl group, an aryl group or a halogen atom; Z.sub.a and Z.sub.1a, which may be the same or different, each represents the non-metallic atomic group necessary to complete a 5- or 6-membered nitrogen-containing heterocyclic nucleus; X represents an inorganic or organic anion; R.sub.a and R.sub.1a each represents an unsubstituted or substituted alkyl group; and R.sub.2a represents a carboxy-containing substituent.
- 5. The method of claim 2 wherein said merocyanine dye comprises a carboxy-containing substituent on the heterocyclic nucleus of said merocyanine dye.
- 6. The method of claim 5 wherein said merocyanine dye is represented by formula: ##STR9## wherein pb represents an integer of 2 or 3; nb represents an integer of 1 or 2; Z.sub.b represents the non-metallic atomic group necessary to complete a 5- or 6-membered nitrogen containing heterocyclic basic nucleus; Q.sub.b represents the non-metallic atom group necessary to complete a 5- or 6-membered nitrogen-containing acidic heterocyclic nucleus; and R.sub.b represents an unsubstituted or substituted alkyl group.
- 7. The method of claim 2, 3, or 4, wherein said carboxy-containing substituent is represented by formula:
- --P.sub.i --Qa.sub.j --COOH
- wherein P represents ##STR10## --CO--, --O-- or --S-- wherein R.sub.4 represents a hydrogen atom, an unsubstituted or substituted alkyl group; Qa represents an unsubstituted or substituted alkylene group, an unsubstituted or substituted arylene group, an aralkylene group, an alkarylene group, a dipeptide residue or a tripeptide residue; and i and j each represents 0 or 1 which may be the same or different.
Priority Claims (2)
Number |
Date |
Country |
Kind |
55-120594 |
Sep 1980 |
JPX |
|
55-120595 |
Sep 1980 |
JPX |
|
Parent Case Info
This application is a continuation of application Ser. No. 298,815, filed Sept. 2, 1981, abandoned.
US Referenced Citations (8)
Continuations (1)
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Number |
Date |
Country |
Parent |
298815 |
Sep 1981 |
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