Claims
- 1. A method for delivering a polynucleotide into a human stem cell, said method comprising the steps of:
associating the polynucleotide to a human sperm cell through a linker; and effecting in vitro fertilization of a human oocyte with the human sperm cell to form a zygote.
- 2. The method in claim 1 further comprises culturing and establishing an embryonic stem cell from the zygote
- 3. The method in claim 2 further comprises:
screening cells derived from the embryonic stem cell for immunological compatibility with a patient.
- 4. The method in claim 2 wherein the embryonic stem cell has the ability to differentiate into different cell types selected from the group consisting of: myoblasts, hematopoietic stem cells, and neural stem cells.
- 5. The method in claim 1 wherein the polynucleotide is able to express a gene product selected from a group consisting of an RNA or a protein.
- 6. The method in claim 1 wherein the polynucleotide is associated with an external surface of the human sperm cell through the linker.
- 7. The method in claim 1 wherein the linker is an antibody.
- 8. A vector comprising:
a human sperm cell; and a polynucleotide linked to the human sperm cell through a linker.
- 9. The vector in claim 8 wherein the polynucleotide is introduced into a human oocyte during fertilization by the human sperm cell.
- 10. The vector in claim 8 further comprising an embryonic stem cell derived from fertilization of the human sperm cell with a human oocyte.
- 11. The vector in claim 8 wherein the polynucleotide is able to express a gene product selected from a group consisting of an RNA or a protein.
- 12. The vector in claim 8 wherein the polynucleotide is associated to an external surface of the human sperm cell through the linker.
- 13. The method in claim 8 wherein the linker is an antibody.
- 14. An embryonic stem cell derived from fertilization of a human oocyte with a human sperm cell linked to a polynucleotide through a linker.
- 15. The embryonic stem cell in claim 14 wherein the embryonic stem cell is immunologically compatible with a patient.
- 16. The embryonic stem cell in claim 14 wherein the human sperm cell and the human oocyte are derived from biological parents of the patient.
- 17. The embryonic stem cell in claim 14 wherein the polynucleotide is able to express a gene product selected from a group consisting of an RNA and a protein.
- 18. The embryonic stem cell in claim 14 wherein the embryonic stem cell has the ability to differentiate into different types of cells.
- 19. The embryonic stem cells in claim 18 wherein the different types of cells are selected from a group consisting of myoblasts, hematopoietic stem cells, and neural stem cells.
- 20. The embryonic stem cells in claim 14 the polynucleotide is linked to an external surface of the human sperm cell.
- 21. The embryonic stem cells in claim 14 wherein the linker is an antibody.
- 22. An antibody characterized by having binding affinity to a sperm cell and wherein a sperm cell bound antibody retains the ability to fertilize an oocyte.
- 23. The antibody in claim 22 wherein the sperm cell is a human sperm cell.
- 24. The antibody in claim 22 wherein the sperm cell is selected from a group consisting of mouse sperm cell, cow sperm cell, pig sperm cell, chicken sperm cell, sheep sperm cell, and goat sperm cell.
- 25. The antibody in claim 22 wherein the binding affinity to sperm cells is further characterized by the ability to bind to sperm cells from a plurality of species of animal.
- 26. The antibody in claim 22 also exhibiting binding properties to a polynucleotide such that upon fertilization, the polynucleotide is introduced into the zygote.
RELATED APPLICATIONS
[0001] This application is a continuation-in-part application claiming priority to U.S. patent application Ser. No. 09/537,861, filed on Mar. 28, 2000. U.S. application Ser. No. 09/537,861 is hereby incorporated by reference as if fully set forth herein.
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
09537861 |
Mar 2000 |
US |
Child |
09781046 |
Feb 2001 |
US |