Method for determining the presence of at least one allele of a GSTT1 deletion mutant

This invention relates to a method for detecting the presence of at least one single allele of a deletion mutant, a PCR assay for detecting the presence of at least one GSTT1* allele and a procedure for diagnostic testing of patients to check whether they are susceptible to toxins or resistant to certain therapeutic agents or belonging to risk groups.

Human glutathione S-transferase theta (GSTT1) is an important detoxification enzyme comprising a deletion polymorphism. Approximately 20% of Caucasians are homozygous GSTT1*0/0 failing to express any GSTT1 activity. Non conjugators may have an impaired ability to metabolically eliminate toxic compounds and may therefore be at increased risk for cancer, inflammatory diseases or chemical poisoning.

Any conclusion drawn from current genotyping was limited because heterozygous (*A/0) and homozygous (*A/A) samples could yet not be discriminated. Phenotypically suggested high- and intermediate conjugators remained genotypically unexplained. The classification of all three genotypes has so far been hampered by the elucidation of the correct molecular mechanism of the GSTT1 deletion.

Thus, it is the object of this invention to provide a method for detecting the presence of at least one single allele of this deletion mutant.

This problem is solved by a method for detecting the presence of at least one single allele of a deletion mutant, wherein a PCR is performed with two primers, of which one stems from the sequence upstream of the deletion area, and the other stems from the sequence downstream of deletion area, wherein the production of the corresponding DNA fragment in a PCR is checked.

It is preferred to use this invention for detecting the presence of at least one GSTT

1

*

0

allele, wherein a combination of one primer from the enclosed sequence

1

and one primer from the enclosed sequence

2

is used and specific DNA fragments for the GSTT

1

*

0

allele are obtained by PCR.

Thereby, using the following primers showed very good results:

CAG TTG TGA GCC ACC GTA CCC

(SEQ ID NO: 21)

CGA TAG TTG CTG GCC CCC TC

(SEQ ID NO: 22)

A special purpose of this invention is to use the invention in a procedure for diagnostic testing of patients to check whether they are susceptible to toxins or resistant to certain therapeutic agents or belonging to risk groups, wherein

blood samples from the patients are obtained and genomic DNA is prepared from these blood samples

a PCR-Mapping of the obtained DNA is performed using a combination of one primer from the enclosed sequence 1 and one primer from the enclosed sequence 2 and

it is analysed whether corresponding DNA-fragments have been produced by the PCR.

In a preferred embodiment, there is an additional PCR mapping of the obtained DNA using a primer pair from within the GSTT1 gene and it is analysed whether PCR fragments of the primers according to claim

3

and/or PCR fragments of the primers from within the GSTT1 gene have been produced. In this way, all possible allele combinations (GSTT1*0/0, GSTT1*A/0 and GSTT1*A/A) can be detected.

It is preferred to use this GSTT1-genotyping assay to predict the risk for UV mediated skin damage and/or the genetic risk for skin cancer and/or the genetic risk for cancers that are associated with oxidative stress and/or damage.

According to this invention, it is further possible to obatin a quantitative PCR assay for detecting the number of active GSTT1 alleles using the ABI TaqMan® technology.

With the method according to this invention, it is preferrably possible to use the knowledge of the exact GSTT1 copy number to develop quantitative PCR based assays for detecting the number of active GSTT1 alleles using the ABI TaqMan® technology.

It is further possible according to the invention to use the knowledge of the exact numbers of GSTT1 copoies obtained according to the invention to calibrate quantitative PCR based assays for detecting the number of active GSTT1 alleles using the ABI TaqMan® technology.

The knowledge of the exact GSTT1 copy numbers obtained according to the invention can also be used to calibtrate assays for detecting the number of active GSTT1 alleles.

In the following, the invention is explained in detail with reference to the enclosed figures and tables, wherein

FIG. 1

shows the structure of the GSTT1 gene region and mapping of the deletion and the genomic organisation of GSTT1 (*A allele)

FIG. 2

shows the mapping of the deletion and primer localization as well as selective primer combinations and corresponding PCR fragments for mapping of the deletion:

Primer Combinations:

lane 1 and 2: GST-TF13/TRF13,

lane 3 and 4: GST-TF12/TRF12,

lane 5 and 6: GST-TR11/TFR11,

lane 7 and 8: GST-TR13/GST-TRF13;

FIG. 3

shows the differentiation of GSTT1 genotypes by PCR assays:

a) Method according to the invention: a 1,5 kb fragment defines the *0/0 genotype, a 1,5 kb and 466 bp fragment indicate *A/O and a 466 bp fragment *A/A.

b) Method according to the state of the art: no fragment defines the *0/0 genotype, a 459 bp fragment indicates *A/0 or *A/A;

wherein lanes 1-3 represent samples from completely deleted (*0/0) individuals, lanes 4-6 from heterozygous (*A/0) and lanes 7-9 from homozygous *A individuals (*A/A), a 100 bp ladder (M) was used; identical samples were investigated;

FIG. 4

shows the sequence and the mechanism of the GSTT1 deletion:

The schematic representation of the recombination event above and the sequence of the recombinant fragment; the positions for sequences for HA5 and HA3 correspond to the positions on the complement of SEQ ID NO: 32 these sequence of H0 is SEQ ID NO 31;

FIG. 5

shows the frequency distribution of GSTT1 conjugation activity in 130 samples;

FIG. 6

shows the mean UV skin sensivity by minimal erythema dose according to GSTT1 genotype in 60 healthy volunteers;

FIG. 7

shows the ratio between GSTT1 and CCR 5 in dependance on the number of PCR cycles;

FIG. 8

shows the clusters with low, intermediate and high flurescense ratios corresponding to the genotypes GSTT1*0/0, GSTT1*A/0 and GSTT1*A/A respectively;

Table 1 shows the primers for the characterization of the GSTT1 deletion locus and diagnostic PCR;

Table 2 shows the GSTT1 allele distribution and phenotype correlation;

Table 3 shows the PCR fragments for GSTT1 genotyping;

Table 4 shows the skin sensitivity to UV irradiation dependent on GSTT1 genotype; and

Table 5 shows the cluster analysis and statistical significance of the genotype correlation.

The invention characterizes the structure and mechanism of the GSTT1 deletion by PCR mapping and sequencing: a 54251 bp fragment carrying the GSTT1 gene was deleted from the functional allele by a homologous recombination event. The deletion breakpoints are concealed within a 403bp region on the null allele.

Based on this date a PCR assay using primer pairs (CAGTTGTGAGCCACCGTACCC (SEQ ID NO: 21), CGATAGTTGCTGGCCCCCTC (SEQ ID NO: 22)) and (CCAGCTCACCGGATCATGGCCAG (SEQ ID NO: 19), CCTTCCTTACTGGTCCTCACATCTC (SEQ ID NO: 20)) for GSTT1*0 and GSTT1*A, respectively, has been established according to the invention, that revealed all three GSTT1 genotypes (GSTT1*0/0, GSTT1*A/A, and GSTT1*A/0).

Furthermore, a Mendelian intermediary inheritance was proved by correlating the GSTT1 genotype with the enzyme activity using the substrate dichioromethane. Samples with two active alleles (GSTT1*A/A) expressed a statistically significant higher enzymatic activity compared to those with one null allele (p<0.0001, ANOVA)

This improved method can be introduced into routine genotyping as a new diagnostic tool and will help to elucidate the clinical relevance of this gene.

Glutathione S-transferase theta enzyme activity involved in the metabolism of toxic compounds is absent in approximately 20% of Caucasians due to a homozygous deletion of GSTT1(*0/0). Because the exact manner of the GSTT1 deletion was unknown, current genotyping of GSTT1 was limited to detect the presence vs complete absence of the gene by a GSTT1-specific PCR. Thus, heterozygous (*A/0) and homozygous (*A/A) samples could not be discriminated. The invention characterizes the boundaries of the deletion of the human glutathione S-transferase theta (GSTT1) gene: PCR mapping and sequencing revealed a 54251 bp fragment including GSTT1 to be deleted from chromosome 22, most likely by a homologous recombination event between two highly homologous sequence stretches that flank GSTT1. Based on the knowledge of the GSTT1*0 region, a PCR assay was devised for unambiguous discrimination of homozygously deleted (*0/0), heterozygously (*A/0) and homozygously GSTT1 carrying (*A/A) individuals. Genotyping of 180 samples of a Caucasian population revealed that the deletion consists of one defined allele, whose distribution in the population fits the Hardy-Weinberg equilibrium with observed 20% *0/0, 46% *A/0 and 34% *A/A individuals. The number of GSTT1*A alleles detected by this procedure correlated highly significant with the enzyme activity in erythrocytes. Genotype-phenotype comparisons proved a codominant type of inheritance by a gene-dose effect: samples with two active alleles expressed a statistically significant higher enzymatic activity compared to those with one null allele (p<0.0001, ANOVA).

Glutathione S-transferases (GSTs), a multi gene family of enzymes comprising several classes (class alpha, mu, pi, theta, and zeta), are expressed in many tissues, including liver, lung, heart, intestine, erythrocytes, and lymphocytes (Hayes et al., 1995). GSTs regulate the conjugation of toxic compounds to excretable hydrophilic metabolites. Because of that, their activity may affect individual susceptibility to environmental toxins, carcinogens, cancer, and other diseases (Strange 1999). The glutathione S-transferase theta (GSTT1) gene, and its corresponding enzyme activity is lacking in about 20% of Caucasians (Brockmöller et al., 1996). However, even within the Caucasian population, this frequency differs with respect to ethnicity (Nelson et al., 1995). A nonfunctional GSTT1 allele (GSTT1*0) is the result of a partial or complete deletion of the gene, the enzyme is completely absent in homozygots (GSTT1*0/0) (Pemble et al., 1994). Many chemicals, such as halogenated alkanes and epoxides whose use is widely spread in industry, are substrates for GSTT1 and thus their toxicity can be modulated by GSTT1. Interestingly, glutathione conjugation may cause detoxification as well as toxification. For instance, the conjugation of dichloromethane yields the toxic metabolite formaldehyde (Hallier et al., 1994) and the mutagenicity of several halogenated alkanes was enhanced in a GSTT1-expressing model system (Their et al., 1996). Thus, GSTT1 polymorphism may determine individual susceptibility towards toxic compounds. For example, of two workers that were accidentally exposed to methyl bromide, the GSTT1 conjugator suffered severe poisoning while the deficient developed only mild neurotoxic symptoms (Garnier et al., 1996). Since glutathione conjugation may provide a step in elimination of substances which are toxic per se, GSTT1 activity can also act protective (Ketterer et al., 1993). This explains that GSTT1 protected human lymphocytes from DNA and chromosomal damage after exposure to several halomethanes (Wiencke et al., 1995; Hallier et al., 1993). Consequently, GSTT1 has been investigated as risk factor in epidemiological studies. The deletion conferred an increased risk in myelodysplastic syndrome (Chen et al., 1996) and some studies have suggested a correlation with susceptibility of the skin to UV-irradiation (Kerb et al., 1997). Although substantial data on epidemiological associations between GSTT1 deficiency and cancer exist, the results are conflicting, which might be partly due to limitations of the currently used genotyping procedures for GSTT1. Using biochemical analyses, three groups, high-, intermediate-, and non-conjugators, can be discriminated (Hallier et al., 1990). This may suggest a Mendelian intermediary inheritance (Wiebel et al., 1999), but so far the genetic background of intermediate and high conjugators and a gene-dose effect could not be unambiguously established. The currently used genetic assay for GSTT1 deficiency is a gene-specific PCR fragment that is present in conjugators, and absent in GSTT1*0/0. The fragment is therefore diagnostic for the presence of at least one functional allele (GSTT1*A) and a differentiation between homo- and heterozygous carriers of GSTT1*A is not possible, according to the state of the art.

This invention shows the characterization of the GSTT1 deletion, probably the result of a recombination event between two highly homologous regions that flank the GSTT1 gene. Utilizing the sequence of the GSTT1*0 recombination region, a PCR assay was devised that permits not only the unequivocal determination of homozygously deleted (*0/0) but also the discrimination of the heterozygously (*A/0) from the homozygously active (*A/A) individuals. The three GSTT1 genotypes detected by this procedure correlated highly significant with enzyme activity in erythrocytes. The trimodular distribution of phenotypes with high-, intermediate-, and null activity in homo- and heterozygotes for the *A allele and *0/0 homozygotes, respectively, indicates a gene-dose effect.

For initial determination and characterization of the GSTT1 deletion, samples of Caucasian volunteers from the Dr. Margarete Fischer-Bosch-Institute of Clinical Pharmacology in Stuttgart, and from the Institute of Clinical Pharmacology at the University Medical Center, Charité in Berlin have been used. Samples were obtained under consideration of all ethical and legal requirements. Genomic DNA was prepared from blood using the Qiagen (QiaAmp) kits on a Qiagen 9604 robot. For geno-phenotype correlations, phenotyped subjects have been used (n=130, male, mean age 30.7 years, ranging from 22 to 49 years) which were part of a previous study (Bruhn et al. 1998), DNA was obtained from these samples using phenol/chloroform extraction.

Determination of formaldehyde production rate (pmol HCHO/min/μl) from 31, 62, and 124 mM dichloromethane in hemolysate was used as a measure for GSTT1 activity (Bruhn et al., 1998).

NCBI database entries Z84718.1 and AP000351.2 (Genbank) contain GSTT1 sequences in annotated form (Z84718.1) or as raw data files, respectively. DNA sequence comparisons, alignments and the construction of composite files from raw data sequence files were performed using the programs FASTA and BLAST at the NCBI server.

Specific oligonucleotide primers for PCR of GSTT1 gene fragments from genomic DNA were derived (Table 1). Sequences of purified PCR fragments were obtained by automated DNA sequencing on ABI 377 (gel) or ABI 3700 (capillary) sequencers using BigDye Terminator cycle sequencing reactions (PE Biosystems). Amplification of fragments less than 2 kb was performed in 25 μl volume: 100 ng DNA template added to buffer containing 1.5 mM MgCl

2

, 200 μM dNTPs, 0.2 mM each primer and 1 U HotStarTaq polymerase (all reagents Qiagen, Hilden, Germany). PCR was carried out in a Perkin Elmer GeneAmp System 9700 with an initial denaturation of 15 min at 95° C. followed by 30 cycles of 94° C. for 30 s, 30 s annealing and 60 s of extension at 72° C. Final extension was carried out for 7 min at 72° C. For longer amplicons 50 μl PCR reactions contain 200 ng of genomic DNA, reaction buffer 3, 500 μM dNTPs, 2.6 U Expand Taq-System (Roche, Basel, Switzerland) and 0.3 mM primers (Metabion, Munich, Germany). Samples were incubated at 92° C. for 2 min, followed by 35 cycles at 92° C. for 10 s, 45 s annealing at 68° C. for each kb per min extension time. The extension time of each cycle was increased by 20 s for the last cycles. 10 min final extension at 68° C. were applied.

The human GSTT1 gene is located on chromosome 22q11.2 which has recently been completely sequenced in the course of the human genome sequencing project (Dunham et al., 1999); prior to that, partial chromosome sequences were available in public databases from the Sanger Center. A 76799 bp DNA sequence of a BAC clone (gb: Z84718.1) contains the GSTT1 and the GSTT2 gene. Homology searches in unnotated raw sequences revealed one additional GSTT1-containing clone, AP000351.2 (118999 bp) consisting only of preliminary BAC sequences which are not assembled to a defined linear gene. A defined sequence file that contains GSTT1 and the flanking regions, which extends the annotated GSTT1 gene region of clone Z84718.1 was constructed by homology alignments using FASTA and BLAST at the NCBI server. Due to the high homology of parts of the sequences, the stringency of the alignments was set higher than default and the correctness of the assembled sequence was confirmed by visual inspection. The composition and prominent features of the GSTT1 gene region are shown in FIG.

1

: GSTT1 and the homologous GSTT2 gene (55% protein homology, Tan et al., 1995) are separated by 49741 bp. In addition to many repetitive elements, GSTT1 is flanked by 18-kb regions which are more than 90% homologous (defined in the *A i.e. non-deleted allele, as homology region HA5 upstream and HA3 downstream of GSTT1).

Although the detailed site of the GSTT1 deletion was unknown, the homozygous null allele could be diagnosed by the absence of a PCR fragment that is specifically amplified from the GSTT1 coding region (Pemble et al., 1994). The deletion removes GSTT1 but not GSTT2 (Tan et al., 1995). Therefore, one breakpoint of the deletion is positioned between GSTT1 and GSTT2 and the other must be downstream of GSTT1 (FIG.

1

). To map the GSTT1 deletion we determined the presence or absence of specific sequences upstream and downstream of GSTT1 by PCR. Ten samples of the genotype GSTT1*0/0, and ten samples with at least one GSTT1*A allele were preselected (see

FIG. 3

a

). Primer sets for specific amplification of fragments up- and downstream of GSTT1 were generated. This required extensive optimization considering the highly homologous flanking regions of GSTT1. Fragments that could be amplified from both, GSTT1*0/0 and samples containing at least one *A allele (*A) were regarded to be outside the deletion, whereas fragments that could exclusively be amplified from *A samples, but not from *0/0 contain the region which is deleted.

FIG. 2

shows results of this mapping procedure. Primer combinations that bind upstream from position 50191, and downstream from position 110007 of the sequence file can be obtained from all samples. Fragments closer to GSTT1, downstream position 53494 and upstream position 105675 cannot be amplified from *0/0 samples. Therefore, the boundaries of the deletion must be localized within a region between position 50192 to 53493 upstream and 105676 to 110006 downstream of GSTT1.

To home in on the exact positions of the deletion, we applied long range PCR has been applied to span the deletion in *0/0 samples. Various sets of PCR primers were selected close to the deletion boundaries as defined by PCR-mapping. In GSTT1*A samples, the 5′ and 3′ primers were separated by more than 60 kb, in *0/0 samples the distance between these primers was reduced by the size of the deletion. Utilizing different primer combinations, reproducibly deletion-spanning PCR fragments of 10065 bp, 3187 bp and of 1460 bp have been generated (

FIG. 3

, Table 3 for primer positions). The 10 kb fragment was sequenced to characterize the deletion region. The comparison of this null allele sequence with the GSTT1*A allele revealed the boundaries of the deletion (FIG.

4

): it is flanked upstream and downstream by sequences that are part of the highly homologous HA5 and HA3 regions. In the *A allele, these regions flank GSTT1. In *0, the deletion generates a fusion sequence (H0) which differs from HA5 and HA3 by only a few nucleotide deviations as shown in FIG.

4

. Using these deviations as “markers”, for identification of the HA5 or HA3 portions of H0, the region where the deletion had happened could be narrowed down to a 403 bp sequence stretch. This sequence is identical in HA5 and HA3. These data support the assumption that the mechanism which generated the deletion is homologous recombination between the regions HA5 and HA3, which removes GSTT1 and generates H0.

The method according to the state of the art to analyze GSTT1 includes a critical negative test output: lack of a PCR signal defines *0/0 samples, whereas generation of the fragment detects the presence of at least one GSTT1*A allele (*A/A or *A/0). In addition to problems associated with negative test readouts (e.g. false results due to test failure may be misinterpreted as *0/0), another drawback of that method is that homozygous (*A/A) and heterozygous (*A/0) samples cannot be distinguished. Utilizing the molecular composition of the deletion, the invention devises a new genotyping protocol that allows not only a positive detection of the deletion allele, but also permits the unambiguous discrimination of all GSTT1 genotypes (*A/A, *A/0, and *0/0). The procedure generates deletion-spanning PCR fragments, which are combined with fragments that indicate the presence of GSTT1. Various PCR assays were evaluated using GSTT1 fragments in combination with deletion spanning fragments of sizes between 10065 and 1460 bp (table 3). Among these, one assay that had been established was found to be “robust” and allowed a reproducible simultaneous discrimination of all genotypes. The 1460 bp deletion-specific PCR fragment was combined with a 466 bp fragment that detected GSTT1*A.

FIG. 3

shows the results of this assay applied to known *0/0 samples and *A/? samples: all samples that had previously been genotyped homozygous for the deletion by the standard method confirmed the *0/0 genotype with a positive readout of 1460 bp. The genotype GSTT1*A/A is diagnosed by the single 466 bp fragment and can be differentiated from heterozygous individuals who are characterized by the presence of both fragments. Testing more than 50 GSTT1*0/0 samples, no discrepancies were detected between the methods according to the state of the art and according to the invention.

The GSTT1*0/0 genotype correlates with the non conjugator phenotype. To evaluate whether intermediate and high conjugators are caused by *A/0 and *A/A genotypes, 130 samples whose GSTT1 activity in blood had been determined have been genotyped.

FIG. 5

shows that the three GSTT1 genotypes could be assigned to distinct phenotypes: enzyme deficiency in GSTT1*0/0 samples, intermediate activity in *A/0 samples, and high activity in *A/A samples has been observed. The correlation of genotype and phenotype is statistically highly significant, p<0.0001 for all group comparisons (ANOVA with Bonferroni/Dunn correction for multiple testing). The allelic frequencies were in agreement with Hardy-Weinberg's law, the difference between observed and expected results (calculation based on the frequency of *0/0) was not significant (Table 2).

Characterization and Mechanism of the GSTT1 Deletion:

The invention allows to characterize the structure and mechanism of the GSTT1 deletion and identify two 18 kb homology regions flanking GSTT1 which are involved in the deletion (most likely crossing over) event that produced the *0 allele. Extensive sequence identity between both repeat regions in the *A allele, and between these repeats and the corresponding region in the deletion allele allowed to define the deletion boundaries within a 403 bp region. Gene deletion by homologous unequal crossing over has been described in other detoxification enzymes, in cytochrome P450 2D6 and glutathione S-transferase M1 (Steen et al., 1995; Xu et al., 1998; Kerb et al., 1999). Like in GSTM1, the GSTT1 deletion has a high frequency in the Caucasian population. Does this deletion consist of one defined null allele or are there various deletions with the loss of GSTT1 as the common denominator ? Since the assay showed the deletion allele as “measurable” PCR fragment, any variations in the size of the deletion indicative of multiple deletion alleles would have been detectable. In more than 150 *0 allele harboring samples that have been analyzed, the “deletion fragment” showed the same size of 1460 bp, suggesting that all GSTT1 deficiencies are caused by one allele.

Improved genotyping assay and allele distribution in Caucasians: So far, genotyping could detect the absence of GSTT1, but provided neither information about the boundaries of the deletion nor about the precise genotype. In spite of the extreme homology, a few single nucleotide variations specific for the recombinant region allow to create an assay for the detection of the inactive allele by presence of a PCR-fragment. A single PCR assay that detects this deletion-spanning PCR-fragment, combined with a fragment that indicates the presence of GSTT1, allows the unambiguous discrimination of all genotypes. Using the assay, the allele distribution was analyzed in Caucasian individuals and found to be 34% homozygous *A/A, 46% heterozygous, and 20% *0/0. This frequency fits to the distribution that would be expected on the basis of Hardy-Weinberg equilibrium (Table 2).

GSTT1 as risk factor in cancer: A number of epidemiological studies have been published on the medical importance of GSTT1 (Strange and Fryer, 1999). GSTT1*0/0 was found to be associated with brain cancer (Kelsey et al., 1997, Elexpuru Camiruaga et al., 1995), head- and neck cancer (Cheng et al., 1999), lung cancer in Hispanic- and African Americans (Kelsey et al., 1997). However, results were often ambiguous (Duncan et al., 1995, Heagerty et al., 1996) or gave conflicting results in bladder cancer (Kempkes et al., 1996, Brockmöller et al., 1996) and colorectal cancer (Clapper and Szarka, 1998; Zhang et al., 1999, Chenevix-Trench et al., 1995; Katoh et al., 1995; Gertig et al., 1998). In all these studies only a comparison of the null genotype with an active genotype was done. The difference between heterozygous and homozygous active individuals has not yet been elucidated, but determining both, could improve the statistical power in epidemiological studies.

Genotype-phenotype Correlation:

Phenotypic data have indicated the presence of intermediate conjugators displaying only half the activity of high conjugators (Warholm et al., 1995). These observations, and a family study that analyzed GSTT1 by semiquantitative PCR (Wiebel et al., 1999), suggest a gene-dosage effect on GSTT1 activity. In this, it has unambiguously been proven the intermediate Mendelian type of inheritance of GSTT1 for the first time. The enzyme activity of GSTT1 correlated highly significant with the number of functional alleles and phenotypically classified intermediate- and high conjugators were genotypically detected hetero (GSTT*A/0) and homozygous (*A/A), respectively. In only 9 among a total of 130 samples the genotype did not correlate well with the phenotype. Three intermediate conjugators had two active alleles and 6 samples with high enzyme activity displayed unexpectedly only one active allele. Since the enzymatic activity of all discrepant individuals was close to the antimode and the enzyme assay had a CV of 7%, differences are most likely the result of biological variability. With codominant inheritance, each allele confers a measurable, yet variable component to the phenotype resulting in a wide range of enzymatic activities from a distinct genotype and overlapping activities between homo- and heterozygotes. Furthermore, GSTT1 genotype-phenotype discrepancies can be modulated by exposure to inducers or inhibitors, whereas the genotype remains constant. Two other subjects attracted our attention because their extraordinary high conjugation activity was 2-fold higher than the mean of homozygous conjugators (68 pmol/min/μl versus 32 pmol/min/μl, FIG.

5

). These subjects displayed the *A/A genotype in our assay. One possible explanation could be a gene duplication or amplification of GSTT1. Members of the GST multigene family have been evolutionary derived from a Theta-class gene duplication (Pemble and Taylor, 1992), and a duplicated class M1 gene that causes ultrarapid enzyme activity has already been described (McLellan et al., 1997). Thus, it is feasible that rare (2 of 130) ultrahigh GSTT1 activity may be caused by additionally amplified gene copies.

The identification of GSTT1 genotypes with a procedure that unambiguously discriminates *0/0, *A/0 and *A/A alleles predicts highly significant the phenotype and will allow an accurate assessment of health risk from halogenated alkanes or pesticides (Bruning et al., 1997; Au et al., 1999; El-Masri et al., 1999). It also provides a useful approach for the evaluation of the importance of GSTT1 as risk factor for various diseases.

This invention is especially suitable to check whether an individual has a genetic risk for UV mediated skin damage and/or skin cancer and/or cancers that are associated with oxidative stress and/or damage.

Ultraviolet (UV) irradiation by sun exposure and family history are risk factors for the development of cutaneous melanoma. Inherited susceptibility to this type of skin cancer could therefore result from genetic factors that affect the capacity of cells to prevent UV-induced DNA lesions. UV light mediates the formation of radical oxygen species (ROS) such as hydroxyl and superoxide radicals, hydrogen peroxide, and single oxygens. These molecules comprise “oxidative stress” and damage cellular proteins, lipids, and DNA. Oxidative stress can cause inflammation, mutations, and genotoxicity. The skin is equipped with a defense system against oxidative stress (Vessey, 1993). Glutathione S-transferases (GSTs) contribute to this protection either by direct inactivation of peroxidized lipids and DNA (Berhane et al., 1994; Ketterer and Meyer, 1989; Tan et al., 1988), or by detoxification of xenobiotics, which can serve as cofactors of radical formation. Our improved assay system to detect GSTT1 deficiencies and heterozygotes shows that GST genotypes affect the susceptibility of individuals to oxidative or chemical stress.

FIG. 6

shows the results of a panel comparison of two groups of genotypically characterized GSTT1 deficient, homozygous and heterozygous active subjects in respect to sunlight (UV) sensitivity. The GSTT1 genotyping was performed with the assay according to the invention that is described above. Healthy subjects (54 male, 6 female, 18 to 48 (mean 27.6) years old and of German Caucasian origin were selected for the panel comparison study. The constitutional skin types were assigned from tanning and burning histories using the Fitzpatrick's Classification (Fitzpatrick et al., 1987) and the study was performed during the winter months. Reactivity to UV light was determined in eight skin fields (1×0.6 cm) on non-UV exposed buttock skin by increasing doses of simulated sunlight with 20% dose increments (Wucherpfennig, 1931). Before each irradiation, UV intensity of the radiation source (dermalight 2001% equipped with an h2 filter, Dr. Höhnle, Munich, Germany) was calibrated to 1.33 mW/cm

2

at 280-315 nm, by use of a UVA/B Meter (Dr. Höhnle). UV dose [J/cm2) was calculated by intensity [mW/cm

2

]×time [sec]/1000. Dose variations, from 0.07 J/cm

2

to 0.34 J/cm

2

, were achieved by irradiation time. Erythema reactions were scored visually 20 hours post-exposure, independently by two examiners. All skin reactions were photographically documented. The irradiation dose of the first field with a barely perceptible erythema determined the minimal erythema dose (MED). The software package for statistical analyses SPSS 10.0 was applied to evaluate genotype-phenotype correlation and statistical significance (SPSS Inc., Chicago, USA). The dependency of UV sensitivity from genotype was tested with ANCOVA to control for constitutional skin type as confounding variable. Adjustment for multiple testing was done according to Bonferroni/Dunn.

The results of the panel comparison study showed a clear correlation of GSTT1-genotype and UV sensitivity of the skin: The GSTT1-deficient group has a higher inflammatory response after exposure to UV irradiation compared to the group with GSTT1 activity. Subjects homozygous for the GSTT1 deletion mutation required a statistically significant lower UV dose to barely perceptible erythema than those with one (GSTT1*A/0; p=0.067) or two (GSTT1*A/A; p=0.032) functionally active alleles. The erythema reaction did not decrease with the number of functional alleles and was equal in both GSTT1*A/0 and *A/A subjects. Among the 5 most UV-sensitive subjects (MED≦0.10 J/cm

2

) were 4 GSTT1 deficient and the two subjects with the lowest inflammatory response (MED≧0.29) were both of enzyme-expressing genotype.

GSTT1 and GSTM1 are expressed in the skin and deficiencies in these enzymes by gene deletions impairs the capacity of cells to detoxify specific substrates, which include molecules that are generated by oxidative damage. The modification of molecules with reactive oxygens as substrate of GSTs provides one link between GSTs enzyme activity and protection against UV radiation-induced cutaneous damage. GST deficiencies result in increased susceptibility of cells to the consequences of ROS attack, such as inflammation or cancerogenesis (Fahey and Sundquist, 1991). UV irradiation by sun exposure and family history are risk factors for the development of skin cancer, particularly cutaneous melanoma. The fact that the GSTT1 and, to a lesser degree the GSTM1 genotype identifies individuals with increased UVsunlight-sensitivity, suggests GSTT1 and -M1 genotype variations to be among the genetic components that result in the inherited susceptibility or predisposition to skin cancer.

This invention further relates to a method for detecting the number of active alleles of the GSTT1 gene using the ABI TaqMan® technology and to a method for calibrating assays to detect the number of active GSTT1 alleles using quantitative techniques (e.g. TaqMan, Light Cyler, MALDI-TOF). Based on the molecular stucture of the GSTT1 gene a realtime quantitative TaqMan® PCR assay was developed, that amplifies a fragment within the coding sequence, and that coamplifies another fragment outside the GSTT1 gene as internal standard, so that the ratio between the yields of the both fragments (meassured by fluorescence signals) in a certain PCR cycle is independent from factors like template concentraion or DNA quality. Provided that the genomic seuquence of the internal standard is non-polymorphic, the number of active GSTT1 alleles is proportional to the described ratio. The internal standard to be used here is based on a fragment within the coding sequence of the CCR5 gene. Different polymorphic sites according to CCR5 are known (32 bp Del, NT794, 1 bp Del, Arg223Gln, Ala335Val, Cys303Ter), but none of them affect amplification of the internal standard fragment. The quantitative GSTT1 Taqman Method, described in this example, permits automized high throughput analyses of individual GSTT1 genotype. Furthermore, genomic DNA of lower quality than required for long range PCR (e.g. degraded DNA as yielded from paraffin-embedded or formalin-fixated tissue samples) can be used as template. The prerequisite for developing the Taqman assay for GSTT1 genotyping is the knowledge of the exact numbers of individual GSTT1 alleles as described in the previous examples. Without knowing the exact number of the active GSTT1 alleles a calibration of the new assay is impossible. Therefore, this method can be considered as a variation, or a direct consequence, respectively, of the GSTT1 genotyping methods described above.

The ABI TaqMan® technology bases on molecular probes labelled by two fluorescence dyes: one is used as reporter and the other is used as quencher. The probe is placed within the fragment to be amplified. The 5′-3′-exonuclease activity of the Taq polymerase hydrolyses the probe, so that the reporter dye is no longer closed to the quencher dye and a fluorescence signal can be measured after excitation at λ=488 nm. The detected reporter signal ist directly proportional to the amplified PCR fragment. For coamplifying two fragments, two different reporter dyes must be used.

An example for the successful application of quantitative PCR (Taqman) to determine GSTT1 genotypes is shown below. The reaction conditions for the assay, in which the CCR5 gene was used as internal standard, were:

GSTT1

forward primer

GTG CCC TTC CCT TAC CCA TC

(SEQ ID NO: 23)

88589-88570

reverse primer

GGG TAC CAG TAG TCA GGG ACC TTA

(SEQ ID NO: 24)

88494-88517

probe

FAM-ACA GTG TGG CCA TCC TGC TCT ACC TGA-TAMRA

(SEQ ID NO: 25)

88554-88528

positions according to GI: 9937243

CCR5

forward primer

TGG CCT GAA TAA TTG CAG TAG CT

(SEQ ID NO: 26)

804-826

reverse primer

GTG CGT CAT CCC AAG AGT CTC T

(SEQ ID NO: 27)

879-858

probe

VIC-TAA GAG GTT GGA CCA AGC TAT GCA GGT GA-TAMRA

(SEQ ID NO: 28)

828-856

positions according to GI: 2347111

component

amount

template

about 10 ng

GSTT1

forward primer

0.4 μM

reverse primer

0.4 μM

probe

0.2 μM

CCR5

forward primer

0.2 μM

reverse primer

0.2 μM

probe

TaqMan Universal PCR Master Mix (ABI)

1 ×

water

ad 25 μL

cycler

ABI Prism 7700

reaction's volume

25 μL

initial steps

50° C.

2 min

95° C.

10 min

denaturation

95° C.

15 s

annealing and extension

60° C.

90 s

cycle number

35

PCR data were exported as “clipped data” to be analyzed, so that for each well and each cycle one fluorescence signal value was available. The ratios were built between the GSTT1 values and the CCR5 values for each sample and each cycle and then were diagrammed in dependance on the number of PCR cycles (FIG.

7

).

The computerized determination of the GSTT1 copy numbers from the output described in

FIG. 7

was performed on data that were obtained at cycle counts>cycle 25. The calculation of fluorescence ratios from data obtained at cycles earlier than cycle 25 is not sensible, because the initial amplification's efficiency is not the same for both fragments. Between cycles 25 and 30, the amplicon yield is suifficiently high to calculate the ratio. In later cycles the reaction kinetics of the PCR leads to a plateau with no further increase of fluorescence signals, levelling differences between the clusters and therefore making the differentiation between homo- and heterozygous carriers of the null allele impossible. Assay evaluation was performed from data generated at 27 PCR cycles. Cluster analysis from the data output of the quantitative PCR resulted in three distinctive clusters of mean (min to max) signal ratios of −0.03 (−0.05-−0.01), 0.28 (0.23-0.33), and 0.58 (0.48-0.54) (table 5). The three clusters did not overlap and differed statistically highly significant from each other (p<0.00001, ANOVA with Bonferroni-Dunn adjustment for multiple testing) and the assignment to clusters was clear without ambiguity. Calibrating this new Taqman assay by comparing the results with those generated by the “method for detecting the presence of at least one single allele of a deletion mutant” revealed that the three clusters correlated completly with the number of active GSTT1 alleles as indicated by a Spearman-Rho of 1.0 (p<0.001).

FIG. 8

illustrates the clusters with low, intermediate, and high fluorescence ratios corresponding to the genotypes GSTT1*0/0, GSTT1*A/O, and GSTT1*A/A, respectively.

The results of this analysis, performed on multiple samples of known GSTT1 genotype demonstrate the capability of the new method to unambiguously determine the copy number of the GSTT1 allele in individual samples.

TABLE 1

Primers for the characterization of the GSTT1 deletion locus and diagnostic PCR

SEQ ID

Primer positions

Name

Primer sequences [5′- . . . -3′]

Nos

Annealing [° C.]

5′

3′

Current Standard Method

GST-TF

TTC CTT ACT GGT CCT CAC ATC TC

003

66

85920*

85898*

GST-TR

TCA CCG GAT CAT GGC CAG CA

004

85462*

85481*

PCR mapping primers

GST-TF13

CCC TCA CTC AGG GTT AGT GG

005

63

110007*

110026*

GST-TRF13

GAT GCC ACG CGG CTT GTA GG

006

110301*

110282*

GST-TF12

GAT TGG TGG AAG GTG CCG GG

007

63

105553*

105576*

GST-TRF12

CGT GTC TCT ACT TCA AAT TCC ATG

008

105675*

105652*

GST-TFR11

TAA GAT ACC TCA TAA AAT TAA CAG

009

59

53904*

53881*

GST-TR11

GGG AGA ATG GAT AGT GGG GAG

010

53494*

53514*

GST-TFR13

GCA AGA AGA CCA GTG ACT GAG G

011

63

50191*

50170*

GST-TR13

CTG CTC TTC TTC AGC AAC TCA G

012

49778*

49800*

10065 bp *0 fragment primers

GST-TRF13

GAT GCC ACG CGG CTT GTA GG

013

65

110301*

110282*

GST-TR12

CTT TTT CTG GAG CAA ACG CAT TG

014

45986*

46008*

3187 bp *0 fragment primers

GST-TRF13.2

GAG CCA AGA AGT TCT GAG TCT TG

015

65

108037*

108015*

GST-TR9n

ATA TCA GCC AGA GAT CTC TGG G

016

50600*

50621*

Sequencing primers

GST-TRF13.3

GCA TCC CAA TTC AAC ACG TGT TG

017

62

107075*

107053*

GSTT-F.1000

CTT CTC AGC TGA AAC TTC CTC

018

51440*

51460*

GSTT1 deletion assay primers

GT*Af

CCA GCT CAC CGG ATC ATG GCC AG

019

70

85457*

85479*

GT*Ar

CCT TCC TTA CTG GTC CTC ACA TCT C

020

85922*

85898*

GT*0f

GAG TTG TGA GCC ACG GTA CCC

021

52069*/6084**

52089*/6114**

GT*0r

CGA TAG TTG CTG GCC CCC TC

022

107779*/7543**

107760*/7524**

*according to AP000351.2

**according to GSTT1*0

TABLE 2

GSTT1 allele distribution and phenotype correlation

Genotype

*A/A

*A/0

*0/0

N

44

60

26

% observed

33.8

46.2

20.0

% expected

a

30.6

b

49.4

b

20.0

b

Mean (SD) enzyme activity

32.1(10.2)

c

15.0(7.4)

c

3.3(0.9)

c

a

based on *0/0, according to Hardy-Weinberg

b

χ

2

= 0.23

c

p < 0.0001, ANOVA with Bonferroni/Dunn correction for multiple testing

TABLE 3

PCR-fragments for GSTT1 genotyping.

SEQ ID

Sequence

Nos

Position

Size [bp]

Specificity

Comment

GATGCCACGCGGCTTGTAGG

006

45986-46008

10065

GSTT1*0

Long range

PCR

CTTTTTCTGCACCAAACGCATTG

014

110301-110282

ATATCAGCCAGAGATCTCTGGG

016

50600-50621

3187

GSTT1*0

Long range

PCR

CAGCCAAGAAGTTCTGAGTCTTG

015

108015-108037

CAGTTGTGAGCCACCGTACCC

021

52069-52089

1460

GSTT1*0

Standard PCR

CGATAGTTGCTGGCCCCCTC

022

107779-107760

CCAGCTCACCGGATCATGGCCAG

019

85457-85479

466

GSTT1*A

Standard PCR

CCTTCCTTACTGGTCCTCACATCTC

020

85922-85898

TABLE 4

GST-Enzyme

MED (J/cm

2

)

a

Activity

n

Mean

Range

SD

GSTT1*A/A

High

15

0.164

0.10-0.24

0.043

GSTT1*A/0

Intermediate

22

0.157

0.12-0.29

0.048

GSTT1*0/0

Deficient

23

0.129

0.08-0.20

0.027

Total

60

0.145

0.08-0.29

0.037

a

Inflammatory reaction was detected as minimal erythema dose (MED) at 20 h after irradiation with UV

TABLE 5

Cluster analyses and statistical significance of the genotype correlation

Distance from

Number

Cluster

cluster center

1

3

0.00

2

3

0.01

3

3

0.05

4

3

0.00

5

2

0.01

6

2

0.01

7

3

0.00

8

3

0.04

9

3

0.01

10

1

0.02

11

1

0.01

12

2

0.01

13

2

0.00

14

1

0.02

15

3

0.01

16

1

0.01

17

3

0.03

18

3

0.01

19

1

0.00

20

3

0.01

21

3

0.01

22

2

0.00

23

3

0.00

24

2

0.02

26

1

0.01

27

1

0.02

28

3

0.01

29

1

0.01

30

3

0.00

31

3

0.02

32

1

0.02

33

1

0.01

34

1

0.01

35

3

0.02

36

1

0.02

37

3

0.00

38

3

0.02

39

1

0.00

40

1

0.03

41

1

0.01

42

3

0.03

43

2

0.01

44

1

0.00

45

3

0.01

46

3

0.04

47

3

0.03

48

1

0.01

49

1

0.02

50

3

0.05

51

3

0.02

52

1

0.03

53

3

0.00

54

1

0.01

55

3

0.01

56

3

0.01

57

3

0.03

58

3

0.01

59

3

0.01

61

1

0.04

62

3

0.02

63

2

0.00

64

1

0.02

65

2

0.00

66

1

0.01

67

3

0.01

68

3

0.02

69

3

0.03

70

1

0.01

71

2

0.02

72

3

0.00

73

1

0.02

74

3

0.01

75

1

0.01

76

3

0.00

77

3

0.01

78

3

0.01

79

3

0.00

80

1

0.01

81

2

0.01

82

1

0.03

83

2

0.01

84

2

0.01

85

2

0.00

86

2

0.01

87

3

0.01

88

3

0.01

89

2

0.01

90

2

0.01

91

3

0.03

92

3

0.02

93

3

0.02

94

3

0.01

95

3

0.03

96

3

0.03

36

1

5000

DNA

Homo sapiens

1
gggtctggcg ggcaccagtg cgatggtgcg ctgtacttgc ggcacaggta gtaaaggatg 60
gccgcgctgc agaaggggcc ggtcaggggc actgcccttg ccttcctgag tgccactaca 120
tcaaccaccc cggtgtggcc tgggcccaac tgctggggct tccagagcaa agaggagccc 180
aaacggcccc gagaaagacc ttcaccagag ctgtctgtct gacagtcagt aagggctggg 240
aaggagccct gcggggtgag taggagttgg gggctggtgg tataacaaag agtaggccag 300
cagggggaac aacacgtgtt gaattgggat gctgaggtgg gaggatcact tgatcccagg 360
aatttggggc tactgtgagc caagatcaca ccactgcact ccagcttggg tgaaagatca 420
agatcctttt tcaaaaacaa aaacgggggg gcacgatggc tcacacctgt aatcccggca 480
ctttgggagg ccaatggggg cagatccctt gaggccagga gttggagacc agcctggcca 540
acatggtgaa accctgtctc tactaaaatg aaaatacaaa aattagctag ttgtggtggc 600
acacacctgt aatcccagct acttgggaag ctgaggcacg ggagtcactt gaacctggga 660
ggcagaggtt gtagtgagcc aagattgtgc cactgtactc cagcctgggc cacagagcaa 720
gactctgtct caaaaaacca acaaagaaaa acacatgctg aaatacgagg gtaaagggag 780
caaggtaaat ctgaagaaaa gagagtaggg ggttgcaact ggaagaaggg tgggggtgat 840
tggggagtga tgaggcagcc agagacactg tggagtccac ggagggtagc ccctggaggt 900
gcagggaggt tatggactta atgcttaaga ttaggcatta tataagccag ggcatgaaag 960
gatccatctc tctggtgctg gatggagggt gagcccgagg gggcagaatg gacaatgagg 1020
gggccagcaa ctatcgggaa ggttgtggtg tctgggaatg ttggaggcca tggggacaga 1080
gggaagggga tggaggggag acatgcttcg gaggggatgt cctaggcctt gctgattgat 1140
ggctggtgtg ggaacctccg cagcacaagg gctcctttat catcaccagc agcaaccatg 1200
ccaaggtaaa aaggtcaggg catggagaga gctatcggtt aaaaagtggc aggagagaca 1260
gcaactggct gcaagactca gaacttcttg gctgggcacg gtggctcacg cctgtaatcc 1320
cagcactctg ggaggccgag gcggggggat catggggtca ggagatcgag accatcctgg 1380
ttaacacagt gaaaccccgt ctctactaaa aatacaaaaa aattagccag gcatggtggc 1440
gggcacctgt agtcccagct actcaggagg ctgaggcagg agaatggcgt gaacccggga 1500
ggcggagctt gcagtgagcc aagatagcgc cactgcactc cagcctgggc aacagagcga 1560
gactccgtct caaaaaaaaa aaaaaaaaaa aacttctttg gatcctgatc caaacaaact 1620
gccaagaaaa tgtttaggag ataatcatag agttttgaac aggagccaca tattagatga 1680
aatccaggaa ttattgttaa ttttatgagg tatcttaatg gtatcgtagt gatgctacgc 1740
tctatcctag cccaggctgg agtgcagtgg cgcaatcaga gttcactgca gttctgaact 1800
tcctggcctc aagcgatcct cccgtgtcag cctctggaag tgctcggatt ataggcatga 1860
gccaccacac ccagcctgtt gctttttttt tgtttgtttt aagaactctt atctctgaaa 1920
agtatgttcc taaacattta ttgatttatt tacttattta tttttatttt tgagatggga 1980
tctcactctg ttgcccacgc tgaagtgcaa cgacgcagtc ttggctcact gcatcctctg 2040
cctcctggct caagcagtct ttccgcctca gcctcccgag tagctgggac tacaggtgca 2100
gaccaccatg ctggctaatt tttgtatttt ttgtagagat ggggttttgc catgttgtct 2160
aggctaggct ggtcttgaac acgtgagctc aggccatccc ctcacttcag cctctcaaag 2220
tgctagaatt acaggcatga gctggcttct aaacatttat gaatggaatg atggggtgtc 2280
tgggaggcag gggaatagaa atgatgtaaa ctggacccca agttggcaag agtcagagct 2340
gggcgatgga tttgtggggt tcctcgtgtc cctcattagt tagtattcac tctcctttag 2400
tgcacgtgtg agattttcca tggtcaaaca gacaaatgct tgcactgaac ctcccaggag 2460
aagcagagac agatggtgca agggccccag ggaagactta cctttcactt aagataaatt 2520
tcccatcttt gaggctgggc agcttcctga gggggttgat gtcaatgtat cctttgctgt 2580
ggtggtgacc tgggaggggc agggaaggtc tgaggctgtg ggactccagg ggagagagaa 2640
ctgagactcc cagagaccca aacgcctccc tctctatttt ctcaagaaga gggaactgag 2700
gcccggaggg acattgcgtc tcaccccagg tcacagggca aggcagttgc agaaccggac 2760
tgcgatcaga actgctggct cccagcctgc tccaccctag gtttggtgac tcccgtgcct 2820
cctacctgtg tcccaggacc aggacgaccc ttttacccag aagccggagg cctccagtgc 2880
ccacccccaa agctggatct gaaaacacag cctttgaatc acctgaagcc ctgagggcct 2940
gggtcccatc cgcaatccca tcgctctcac tctgtctcca ctttaaggaa gccaggccca 3000
gcacacagct ggacatccaa agggaagctt ctcggacaca atcagggtca tcttaacagg 3060
gaacctgagg tgggggcagg aactgaaact cttcctggac cagccgcctc cagttggaaa 3120
catttctggg ggctccactc gcagcccgtt catttccaca gcttccctgt ctcttcctct 3180
gtgttctaga ggcttctgct tttgcaggct gagcttttgg agtccctctg tgctggggat 3240
ggagttggag cccacccctc tgaccctcac tcagggttag tggagccctg agcctttctg 3300
aacactgggg aggatgggtg tagacggact gtgcacttct gccccctttg ccaacctggt 3360
gggcaggtgc tgagttcaca aggtcctaga atcccacaag gaagccaggg tgcctggtgg 3420
gagcccaggg agtcccagct actgttcctt cccccttctc ctcgaaaagc ctgttcatct 3480
gtggcgtggg gactgtcatt agtgagcact gactaaggta ggctggacaa ggatgcagcc 3540
tacaagccgc gtggcatctt ttccttccct gtggacctct ggggtgattc ccttgtctct 3600
gtctctgctc ctcagaaacg cccctatcag gctgtgcgcg gtggctcacg cctgtaatcc 3660
cagcactctg gaggctgagg tgggcagatc acttgaggtc aggagtttga gaccagcctg 3720
gccaacatgg tgaaacccct gttaaaaata caaaaaatta gctgggcgtg gtggcatgca 3780
cctctaatcc cagctactcg ggaggctgag gcaggagacg cacttgaacc cagcagaggt 3840
tgcagtgagc cgagatagca ccaccgtact ccatgctggg caacagagcg agactccatc 3900
aaaaaacaag aaaaaaagaa aagccgcaat ctgtgtgtcc tgcctccccc caggaccagg 3960
cctgccaggc agcagtggga gttgaccttt cagcagatcc acaaactgaa agttgaactg 4020
gatgtcatgc ttcttcgaga agatgtagac ggcacggcag ggtgctgaca gcaggtccat 4080
gtagagctcc agtgccatgt tgagacacat gccaggcccc acagccgcag ttggccagcc 4140
acagacctgg gcctatgtct ggccagagtc cctggccctg tgccctctcc gatctgggcc 4200
caggatcctg tgttccccag ggaaacctct tgtttccctt tgtgttgtca taaggccagg 4260
aagcctgcaa ttctcacagc atcaaggatt ctaaggaggc ccaggagtag gctggggaga 4320
ggcccgtggc aaaggtgtgg cagccgtgac cctactctcc cccttccacg tgtgcctgtg 4380
ccccgtggtg ccacctcaca gacaccagtc tgagaaggga ttatgcctgg gaattcccac 4440
ggctggattt tcattgcaga acctgacgaa aggggctttg cagggtccag aatgaagagg 4500
aggcaatgag aattatccct ggaggattct agaagtagag gctgggagta tccacaggta 4560
aatcgagcct gaactatgac tagaaaggaa ttgggagaaa gagacacagg tgaatcgagc 4620
ctgaactatg actagaaagg aactgggaga aagagacaca ggaaactgtg agctttggga 4680
gcaatgggga caccaccacc aggaagtcag ggggcactca gccggtgtgt gccacacaga 4740
ggagcctaga aacttcctgg ccttggttgg ggctgcagtg gccagactgt gtacctggtg 4800
gccaaggaag gtaactagag ccccacgtag aggactgagt gccactcact ctatgctgtg 4860
atctaatagg tctaggctga gaaatgggac tgaccccact tctggtgaca gagtaagcct 4920
ggagacaagc gaagagcatg cagtgtgttt attgcagaca gcagggtgca gtggagtggg 4980
ctgcacccac tgcacctgct 5000

2

5000

DNA

Homo sapiens

2
tgtggtttta attttgtttt tcatccttta ttgcaaatgt atactattga tttttgtgta 60
ttaatcttgt accctgcaac cttgctgaac ttattttatt ttattgagat ggagtctcag 120
tcagtcaccc aggctggagt gcagtggctt gatcttggca cactgcaacc tccacctcct 180
gggctctggt gattctcctg cctttgcctc ctgagtagct gggactacag gcatgcgcca 240
ccacgcccgg ctaatttttg tatttttagt agagacgggg tttcaccata ttggacaggc 300
tggtctcaaa cttctgacct catgatctgc ccgccttggc ctcccaaagt gctgggatta 360
caggtgtgag ccaccgtgcc cagccttgaa cttgtttatt agctctaatg gtttttaagt 420
gagttaagaa tttctgtaat gtcatctgtg aatagagatg gttttacttc tttctttctg 480
atctagattc caccttgttc ctttttcttg cctaattgcc ctaataagta gaagtagtgt 540
gaatgaacat tcttgtcctg ttcctgatct taggggggaa acgcttgatc tttcacaatg 600
gatgaagtat ggtgttagtt ataggttttt catagatgct ttttgtcaag ttgaggaagt 660
tcctttctcg tcttcatctg ctgagtaatt ttatcgtaaa aggatgttag gttttgtcaa 720
atgccttctg tgcattagga tgatcatgtg actttctatt aacatggtat gctacactga 780
ttgatttttg tatgttgaac tgcatttgta ctcctgggat aaatcctacc tggtcaaggt 840
gtatgatcct tttaatatgc tgctggattt gatttgctaa tattttgctg aggattttta 900
catctatatt tataaggata ttgctttgta actttatttt cttgtaatgt ctttttctgt 960
ctttggtatc agagtaattc tggcctcata gtatgagttg aaaaatattt cttcctgttt 1020
tatttttttg gaagagtttg tgaaggcttt gtgttagttc ttcaagcttt tggttgaatt 1080
caccagggaa gccatctggt cctgggcttt tctttgtgga atttttaaaa ataattaata 1140
ttttaatctc tttatttgtt acaggcctat taaaatttta tctttcttct taagtcagtt 1200
ttggtagatt gtgtgtttct aaaattttcc catttcatct aggttgtcta aattgtccac 1260
atatagttat tcatagtatt tctaaacttt tgaatttctc tatgaccaat gtgatgtctc 1320
cattttcttt ctttctggtt tccatttcat tttttcattt ttgtttttgt ttgttttttg 1380
agataaggtt cttctatgtg cccaggctgg agtgcagtgg tgcaatcata gctcggtgta 1440
accttgaact cttggactcg agtgatcctc ccacctcggc cacccagtta gctaagacta 1500
caggcttgca ccaccacacc aagctaattt ttttaaaaaa tatatttttt agaaaaaagt 1560
ctcattgtgt tacccaggct gttatcaatc tcctggcctc aagtgatcct tctgcctctg 1620
ctttccaaag tgctgggatt gcaggtgtga gccattgcac ccagcctcca ctttctttct 1680
tggtgaataa tttgaccctt atttttttca cttggctatt ctaagtaaag gtttgccaat 1740
tttgttgatc tttgcaaaga tccaattttt ggttttattg attttattgc ttttctattt 1800
tctatttcat ttatctccat tctaatcttt attatttcct gttttctagt ggtgttgggt 1860
tgagtttgct cttatttttc tagttcctta aggtgtaaag ttaggttatt gattcaagat 1920
cattcttctt taacttatgt gtttacagct ataaattttg ctcttagaac tgcttctgct 1980
gcatcctata aatctgggca tgttgtgttt tcatttttat ttgttcagga tactttttga 2040
tgtcccttgt gatttcttct ttaacccatt tgttgtttaa gagtgtgctg tttaatttct 2100
atgtgcttgt gagttttcta gttttccttc tgttcttgat ttttaacttt attccactgt 2160
gtccagagaa catactttgt gtgatttcaa tcatcttaaa tttgagactt aggaccaaat 2220
atatgctcta tactggagaa tattccatgt gctctttaga agaatgctta ttctgctcct 2280
gtcgggtgga acgttctgtt catgtctagt gtatgcgatt ggttaatcgt gcttttcaaa 2340
ttccctattt ccttgatggt cttctgtagt ttttctatta ctgaaagtgg ggtattgaag 2400
tcaccaacta ttattactca caatgtaacc atatttaacc ctttactttt aaaattcttt 2460
ttggaaactg gaaggatctg taaccaccac ccactcccac atcagaccct atgcatatgc 2520
caactgtctg ctgctcagca gctgtgatgg ttgttccagt tctttattag gcaaagaaca 2580
gttgtttttt tgtttttttg tttttttttt tttaacattt cctttaagga aggtggctca 2640
gattgctaag ccagccaggc cctgcgggac aggctgcgcc tagggtcacc tgctcttctt 2700
cagcaactca gaaatattct ccttgaccat tgaatccaat gttgaaaagt cccagtcggc 2760
caactgcatt agtcgatcat gggcctccct aaagaggcca gagccaatat tcagctccac 2820
ctgcatacgc cactcagcta gcttggagct gttgaggaag acattatagt tggctgccat 2880
gggctgtaga tagacgaaga caaagacgtg gtcagcctgg ggaccagccc cacctgggtc 2940
ctctcccaca gcctcaggcc cacgtcccta acatctacct atgcaaagaa ttaggctgct 3000
gacccccaag gcctgagcaa ggggtcacag actatgtaat agatgtaggt ggggatggag 3060
gatccagaat ccctcagagt cctcagtcac tggtcttctt gctccaagcc ttctgaacca 3120
tgctgagagg gctcctggcc caggcacttc ccacttctcc agcctgcgac ctcgcatgaa 3180
tcctgccttc ttccagggaa accccttatc ccaggtgtgt atatgtggat gagggagaga 3240
actcaggttt tttcctccat gtttagcctc ccactgtgat caagctcagg ggctaggatg 3300
ggagacctgg cgggcagtct accctgcagt ttcgctggct tactaagagt ttggtttgca 3360
cccaagatct ttgggaagcc caagaatggg tgtgtgtggg tgaaatgtaa ggggtgggga 3420
cgaagcatat ggctgaaccc ttggggcagg ccagaatgat ttttcctggt gctggtctgc 3480
cctgcaaaca gaccaaggag actaattttc atatcagcca gagatctctg ggataaggaa 3540
aagaatactg cattttctgg tcaatccacc aggaccccag gtccctcctc tcgggcatat 3600
ctctggctga tatgcaaatt agtctctttg gtcagtgtgc agtgccctag ctggtgtgca 3660
ggatggccag ttgagaccct ggccagtgtc ttgacaagca gaactggtca ccctcccctg 3720
catgtagagg ccacataaat gccccacact caggtgtgcc tccaaatgca cagtggatgc 3780
ccctcagacc cagccacgag agctgtcctc cagagctgtc tgtctggagc tctgggaaac 3840
aggcagggcc agaaggacac ccaggaagcc agtgaacatt tcctggagag tccagcaaga 3900
ggaggaggta tctgggatgc tggtggattg agcaggaaat gcagtgttct tctctatccc 3960
aggctcaccc tccgggtcct cccacaccga agaatctttg tcaagtgtgg agaactgtga 4020
tccttcctga ttcataacat tctgtgcttc ctgttgcccc gattgagtcc aggcccccag 4080
gcctggttcc cgcagccccc atggcagctc tgcctgcctt tcccgcctca ccagcctatc 4140
ctcaagtgat ggccccattg gtcacagagg agtcctacct ctgcccaggg tctaaccctc 4200
ctccaatcca ctccacacct gcatcatctc caccacggcc accaggtcag ccagtgagat 4260
ttggttcccg gtgatgaaca tcttatcctg cagaaaatac tcctcaaaga gctgcaggct 4320
gttcttcacc tcttccactg catgctccat cttctcagct gaaacttcct cccctgttat 4380
ctttgggatc agcaactggc cagggttggg aagaggaggg aagaggaggc tgcactccag 4440
ggccacctgc cctgccaggt ctctgtactc ttgtctgctg gatagatatt gaacacttcc 4500
caggatataa agcagtttca cctcttttag cagttctgat tggtggaagt tgctgggaac 4560
catgtgttca caaggatttg gggagctcag caggcataag tcctgtgatt gattagtgat 4620
gtctgtcaca ggcatagaat tcaaagtaga gacacatgta ctggttattt gtcatcttct 4680
aattttctat aggccatact cttttttgtt tttgtttttt gagatggagt ctcactctgt 4740
cgcccaggct ggagtgcagt ggcacaatct tggctcactg ccaactccaa ctcctgggtt 4800
caagcaattg tcctgcctca gcgtcctgag tagctgggat tacaggtgcc catcaccaca 4860
cccagctaat aattttgtat ttttagtaga gatggtgttt cacaatgttg gcaaggtagg 4920
tcttgaactc ctgacctcaa gtgatctgcc cgcctcggcc tcccaaagta ctgggattac 4980
agttgtgagc caccgtaccc 5000

3

23

DNA

Homo sapiens

3
ttccttactg gtcctcacat ctc 23

4

20

DNA

Homo sapiens

4
tcaccggatc atggccagca 20

5

20

DNA

Homo sapiens

5
ccctcactca gggttagtgg 20

6

20

DNA

Homo sapiens

6
gatgccacgc ggcttgtagg 20

7

20

DNA

Homo sapiens

7
gattggtgga aggtgccggg 20

8

24

DNA

Homo sapiens

8
cgtgtctcta cttcaaattc catg 24

9

24

DNA

Homo sapiens

9
taagatacct cataaaatta acag 24

10

21

DNA

Homo sapiens

10
ggcagaatgg atagtgggga g 21

11

22

DNA

Homo sapiens

11
gcaagaagac cagtgactga gg 22

12

22

DNA

Homo sapiens

12
ctgctcttct tcagcaactc ag 22

13

20

DNA

Homo sapiens

13
gatgccacgc ggcttgtagg 20

14

23

DNA

Homo sapiens

14
ctttttctgc accaaacgca ttg 23

15

23

DNA

Homo sapiens

15
cagccaagaa gttctgagtc ttg 23

16

22

DNA

Homo sapiens

16
atatcagcca gagatctctg gg 22

17

23

DNA

Homo sapiens

17
gcatcccaat tcaacacgtg ttg 23

18

21

DNA

Homo sapiens

18
cttctcagct gaaacttcct c 21

19

23

DNA

Homo sapiens

19
ccagctcacc ggatcatggc cag 23

20

25

DNA

Homo sapiens

20
ccttccttac tggtcctcac atctc 25

21

21

DNA

Homo sapiens

21
cagttgtgag ccaccgtacc c 21

22

20

DNA

Homo sapiens

22
cgatagttgc tggccccctc 20

23

20

DNA

Homo sapiens

23
gtgcccttcc cttacccatc 20

24

24

DNA

Homo sapiens

24
gggtaccagt agtcagggac ctta 24

25

27

DNA

Homo sapiens

25
acagtgtggc catcctgctc tacctga 27

26

23

DNA

Homo sapiens

26
tggcctgaat aattgcagta gct 23

27

22

DNA

Homo sapiens

27
gtgcgtcatc ccaagagtct ct 22

28

29

DNA

Homo sapiens

28
taacaggttg gaccaagcta tgcaggtga 29

29

25

DNA

Homo sapiens

misc_feature

Sequence of Figure 4 5? region HA3

29
tgtccgcact cgtgacgagg accgg 25

30

26

DNA

Homo sapiens

misc_feature

Sequence of Figure 4 5? region HA0 and HA5

30
tgtcaacact cggtggcatg ggccgg 26

31

548

DNA

Homo sapiens

31
tgtcaacact cggtggcatg ggccggaaga cacccggaaa actgcgactc taagagatca 60
agtcttactt tacggagctt acgacaggac ccactcagtg tagtcgggga gggcatacgg 120
gaaggggagc gggattattc tgagaaagta cgggtaacaa agtcaggtgt gaggactgcc 180
gagacattcc gcccgtcctc ttcctcgact cctttactcc gggtctctcc ctccctctga 240
acgaactcca gcggccgtca gtcgtcccgg tcttgaccgg aggtcggaag gactgaaggg 300
acacgggcac aggggttcgg ggtcttcacc gggacgagtg gaactcggtc tgatagaaga 360
agtacccgtc gactttccgg cacacaacgg ttcggtgctt gagtaggtgt gcccgtgcac 420
gcacgcccag accgcccgtg gtcacgctac cacgcgacat gaacgccgtg tccatcattt 480
cctaccggcg cgacgtcttc cccggccagt ccccgtgacg ggaacggaag gactcacggt 540
gatgtagt 548

32

118999

DNA

Homo sapiens

gene

(1)..(118999)

Homo sapiens genomic DNA, chromosome 22q11.2,
clone KB226F1, Genbank accession number AP000351.2 GI5420485

32
aagctttaaa ccaagtgcat ggcccttctg cgttgtaggc ccacaaggcc gacactgcta 60
ttcacatcct tgtatggtcc cctaccctgg agcgtggctg gggcctgtgc tctactgctt 120
ggaagcaagg cttatcccta agccttattt tcgggaaggt ggatttgaga gctactatcc 180
cacatccttg cttggtgccc tgtgaataaa tcttttctct tttgcaaaac tcgtgtcatg 240
gtgattaatt tactgtgcaa tgccaggcac tgttggaaac ctctggatag gcctcacagg 300
gcctcagcca tctcggggag gcgttagcta gagtgtaaca aatatgaccc cagcaactgg 360
ggagggacag ctcattcctt catgaggtgt ggggctggca gccccgcagg cagtcagcca 420
cccttcctct ctggcctccg tcctgggcac tgggcccagg cactgggcaa ggaggctgct 480
gcacctcctt tctcctcttc cttttttttt attttttgag acggagtatg gctctgccgc 540
ccaggctgga gtgcagtggc gtgagctcgg ctcactgcaa gctccgcctc ccgggttcac 600
gccattctcc tgccccagcc tcccgagtag ctgggactac aggcgcccgc caccccgccc 660
ggctaatttt ttgtattttt agtagagacg ggtttcaccg ttttagccag tatggtctcg 720
atctcctgac ctcgtgatcc gcccccctcg gtatcccaaa gtgctgggat tacaggcgtg 780
agccaccgcg cccagcctct cctcttccct tttagtggga aatatacctg cagcctagat 840
tcccagaaag gggtggtgag agagcagaca cgccactgat tgacccgaag ttctaggagc 900
caagggatga gtgaagaggg cgtggccgga ctcggggctc ctggtgaggt gcaccgctgc 960
aggctcggtg acccagcagt tcctgcccaa ccctcaccta cccccagccg ttcctgtccg 1020
ccgcccccaa ccagccattc ctgcccgcag ttcctgttcc cgggcctgtc cccgggtttg 1080
tccccagggc ccgtcccctg ggtttaggtc tgccctccca cgccccaccc cattgccccc 1140
gccatgggtc tgctgtccca gccctgccgt tcagtctaca tcttcgccaa gaatggcatc 1200
cccttcgagc ttcgcacagt ggagctgacc aaagctgggc tgggcaggca ggcccagggg 1260
atattggccg cggatccctg cctgtccccg ctgctttgca gttgggccaa gaatgcagac 1320
taacacgaag aatcagactc tccggtgtgg aaactattac ataattatct atgttattat 1380
ttttactttt tggagacagc atctaactct gtcactcagg ctggagtgca gtggcacaat 1440
catggctcac tgcagcctcg accttccagg cttaagcgat cctcccgcct cagcccctca 1500
agtagctggg actacaggtg cccaccgaca tgcctggcca atttattatt attattatta 1560
ttgagaggag gtcttgctgt gtttccgagg ctggcctaga actcctgggc tcaagtgatc 1620
ctcttgcctt ggcctcccaa agtgttggga ttacaagcat gagccgccat gccccactgg 1680
ttgtctattt taatagaggc ttgatctttg cttgtgaata caaaacattc ttcaaaatac 1740
aggtttagag agggcacagc aggcacagaa cgagggtagg gaaatggagt ggggcaagaa 1800
tatcttcacc ccatgtgctt ggaaaaaaca atgaaactgc ctcgttgttt cctaagtaaa 1860
atagtccctg tctacccctg cctgtgtctc ttccactttt gtattatttg tttgtttatt 1920
tatttattta tttatttatt tatttattta tttatttatt acagtgtctc aatctgcagc 1980
ccaggctaga gggcagtggc atgatcccgg ctcactgcag ccttgacctc tcaggatcaa 2040
gtgatcctcc caacttactc ctcctaagta gaatagttgg gactacaggc acgtccccgt 2100
gcccagctac ttttttcttt ggtgtgtgta gacgcatgat tttgccatgt agaccaggct 2160
gaccttgaac tcctgagctc aagtgatcct cctgcctcct ccttctaaag tgttgagatc 2220
acagacatgc accaccatgt ctggccctgg gtctcttcca cttttatgta tttcttggca 2280
ttcttctgcc ttttcctttt cctgcatctg acttgctcta tcccatgggg cttctcaccc 2340
agctgaaggg gctactccta gaggctagag gctggagggc cctgtggcag ggcagtgggg 2400
gaggccagca ccctcaccct gagctccagc gtttgctggt ctggctggca tacagagatg 2460
cagctggagc acaattcagt ggaggaggca gggctataac taagcagtag ggcagagagc 2520
agagccagag ccccctgggc aggccagagg gaggaccctg ctcagctgac tcttcccaac 2580
tgctgccctt ggtacaacca gcttgagtca cctgctacag aacaggggct ggacacagaa 2640
gcagtctgtg gagggagaat gggcaggggt gtgtatgtcc attcagtaga agggcaaaga 2700
ggcccacatg taggcctatg atcagactgg agcctggctt gactgaggtc ctctgatcca 2760
aacaccaggc acctaatgcg gcctctagca ctggcctaga gtgctaaagt gagtcagacc 2820
tgggaacccc cactggggag ggagccttga gagtttggca taagaaagca agaagagctg 2880
agccaccgga ggctaagtag ctgaatgggg cagggattcc cagcaagaat gaggttggtc 2940
tgagggttgg gaacatgttt ggctgggact ctggggtatt gacaggtcac ccagcctggc 3000
cagtagggcc taagtcccca gctgggatag gaagctgaat cagagggggc aagcctagag 3060
cccagggagg aggctggagg ggtctgacct atggatggaa ctggctgggg gtggggtgtg 3120
aattacagct ccagggaatt gtccttgagc catgcttcaa ggagctggac acaggaaaga 3180
tggccctctg atggggtcaa ggtctgtgac ccagtgacta gcagagaggc ttcctcttcc 3240
ctgatgcctc ccagataggg gcctcagaaa gctgctgctg ggattttggg gtgggggtgg 3300
gcagagacct cagagaactc tgggttatcc ccactcacct ctctgatctt tcctgcaggc 3360
cagcagcaca gcgatgcctt tgcccaggtg aaccccctga ggaaggtgcc gggccttgaa 3420
ggatggggac ttcaccttgg ctgagaggta accggtccgt gggctgctgc cgggccttgt 3480
ggggccagtc ggctgtctgt tcactgttga tggcttggat catggactgt gggccctgca 3540
ccagccccag ggtgaagaat gggtggaggc aggcagaggt gcccagtgtt gacaagggtt 3600
atagaagtcc cctcctgcat tgtacaagtg aggaaactga ggctggagga ggggagggac 3660
ttgcacaagg ccacagggcc cagtctgctt ccgggcctcc tcaccgccag ccttcccctc 3720
cgaaaaaagg agagtgctaa gtttcgagga gcggctgtac caggcatctt agtgctacca 3780
ttttgcccac aaatcctgtg gggcaagtgc tgtttccctg gagagtcgag ggaccactca 3840
gcgagcttca gtggctattt gctaaacatc caccagggcc agaccccatt taagccctgg 3900
ggcttgagca gtgaacgtgt ctgcgtcctt ctcctcatgg atctcacatt ccattaatag 3960
aagggggagc tgacagtaac ccatgtaaac atggaacata tcggatgttg atggatgcca 4020
tggaaaaaat aaacagggta agagtggggg tgtgtgtcat tatgagtagg atgctgaggg 4080
aagactccct ggaggaggtg gcatctgagc agggagctgt aggaatgtgc aaggcatgag 4140
gagaagggtg ttctcactgg agggaagagt gagggcgaag gctccaggtg ggacaatgca 4200
tggggttcag actggagagc aacgggccag ggtgggtgca gtgagcagga agagcaggag 4260
gtcacgacag aggggagcat gggacactgg gaaggctttg gctcttaccg caggtggatt 4320
gagttgttgg agagttttga gcagaggaga actgtgatct gacttacgtc gtaaaggatc 4380
cccctggcag gggtgagaaa gccactgcag tcatctggca tgcagcgctg gagccccaga 4440
ccaaggtggt ggtggtgcag ggttgcgggg gattgtggtc agttctgact tttattgaaa 4500
tctaactgca gggtttgctg aagggttgga ggtgggtggg aaaaatgaaa gccaaaacca 4560
gcctccaggt tggtggccgc atggtggcgt cctttaccga ggccacatag tgcctccttg 4620
gtggagctgg attcacattc agagcctggg atcactggat ggtccaacac ccataaaggc 4680
atgatgagct ctgagggtca gactgcacaa cccttcacca taggtgaacg ggagttggga 4740
cagggtggga agcaatgctg ccatgtcacc tggaaggctc tgtccctgct ggccttgctc 4800
ctggccctgt ccacactttg gatatgttgg ggagatatcc aacatgctct cgttcttcag 4860
actcacactg ggtgcccctt cctcctggaa gccttccctg agcccctgct gaagttcatg 4920
acattttgtt tcttccttgc tgcccttagt tgagatgaca gtctggcccc gtgtagggct 4980
gtgtgaccct gggtagttac ctaacctctc tgagcctcac atccctcctc tgcatcatgg 5040
ggatagtgaa aatgccccag aaaacggttg aagagtgttc agggcatgtc ttctgcccct 5100
gccatgtccc cccagtgtgg ctagcttgct ctatatgagc tgcatgtaca aagcacctga 5160
ccactggtac ctccaggacc tgcaggcccg cgcccatggg gatcagtacc tgtcatggca 5220
gcacatggcc ctgcagagta gctgctgctg ggccatgtgg cagacggtga gccatggagg 5280
gcagggacac ctcccgggat gcccaaggga tgctgctttc actttacaaa attctgttta 5340
gacctgagac caaatcctgg aatgccagag actgcctaat tcacccaact tgtttcatag 5400
gagggaaacc aaggaccaga gccaaggaag gattccccta atgtgcctta attccacagc 5460
taccacctgc acacttacac tcacacactg ttcgacacac agccacactc acacacacat 5520
ccacccacgt ccacccacag tcacacgatg tatcacattc acacgtatac acccacacac 5580
acaatcataa catttacaca cagtcacaca gttaactcct atacacacat atacacccac 5640
acaatcacac aactcactca tattcaacaa agtcacacaa ctcactcaca ttgacacaca 5700
tacacaccaa ccccacacac aattcacata catacacaca catatacacc cacatacaca 5760
accacaccat tcactgacat taatacaaag tcatcacaca gttcactccc acacacacac 5820
atacacctca cacacacagg cacacaattc acaccaacaa aatcacacag ttcactcatt 5880
cacacacata cacccacata cacaaattca cactgctcag tcacatttac acaccattca 5940
aattcacaca catatacacc tacatacaca cagttcactc acatcacaca catatacacc 6000
cacatacaca cagttcactc acatcacaca catatacacc cacacataaa cacagtcaca 6060
caattcactc acgttcacac acacagttca ctgacattcg cacacataca cacacaccac 6120
acacaggcaa ttcagtcaca tggccgaaag ccagtcctta ccttcctata gttcctttcc 6180
cagtgtcacc tcagcctttg gccctcagcg tccccttccc atgcctggac tgcggaagag 6240
ccctgaacac accccatctt catgcctcca gcgctagccc gagcccttcc ctctgccaag 6300
agcactaagt gcaactcaag atcacttcta gggacccttc cctgcccctg ctgccaggtg 6360
acctgaggat cctgctggtc ccgcttgaca atgatgcaca gcatggttag ttcagctgag 6420
gtcgctctcc cagacttact gccacctaca cagcactagg actgactgtt taactttatt 6480
ctttatttcg tgtactttat tggagtgtct gttgaaagca aaaccatcca ataaggaaca 6540
atcccaaggc agccagcatg gatcaagaag gcacaggctg tggccgccat ccccagtgcc 6600
tgatggggag agggggtggt aatggaaaag gcccaggaaa gcaggccaga gtgtccctca 6660
aaatatgaga aatcactgga agagagagcc agaatgggac ctaagacaga agccaccacc 6720
aggcaccctt aaggcaggac agttgcaatg gttacttgga atacttccgg ctaggagtgg 6780
tgttgcggga agtcagggac cccaaacaga gggaccggct gaagccatga cagaaaaacg 6840
tggattgtga agattttatg gacatttatt agttccccaa attaatactt ttataatttc 6900
ttatacctgt ctttactgca atctctaaac ataaattgta aagatttcat ggacacttat 6960
cacttcccca atcaataccg ttgtgatttc ctatgcctgt ctttacttta atctcttaat 7020
cctgtcaacc aaggaggatg tatatggcct caggaccctg taataattgc attaactgca 7080
cacattgtac aggatgtgtg tttgagcaat atgaaatgtg ggcaccttga aaaaagaaca 7140
ggataacagc aattgttcag ggaataagag agataacctt aaactctgac tgctggtgag 7200
ccaggcggaa cagagccaca tttctcttct ttcaaaagca aataggagaa atatcgctga 7260
attctttttc tcagcatgga acgtccctga gaaagagaat acgcaccagg aggtatcagc 7320
ttataaacag cacccctggg cgtggcctgt ctcttatggt cgagactgca gagatgaaat 7380
atactccagt ctcccatagc actcccaggc ttattaggaa gaggaaattc ccacctaata 7440
aattttggtc agaccagttg gtctcaaaac cctgtctcct gataagatgt tatcaatgac 7500
aatggtgccc gaaacttcat tagcaatttt aattttgcct cggtcctgtg gtcctgtgat 7560
cttgccctgc ctccacttgc cttgtgatat tctattaccc tcttaagtac ttgatgtctg 7620
tcactcacac ctgttcgcac actccctccc cttttgaaaa tccctaataa aaacttgctg 7680
gtttttgtgg cttgtggggc atcacggatc ctaccaacgt gtgacgtctc ccccggatgc 7740
ccagctttac aatttctctc ttttgtactc tctcccttta tttctcaagc cggctgacgg 7800
ttaggaaaaa taggaaagaa cctacgtgat tattggggca ggtcccccga tagagtggca 7860
catgatgggc acagggcagg cttgggaaat ggggggccgt aaggccacaa gacatgtggg 7920
caggtagccc agagtgtgtc tggaacatcc tgtttattgg tcggcagtga catctactgt 7980
cacatttgag aactgcatcc acagccacat gacctcctgt gaagacctca gggcaaactg 8040
gggaaacctg cccttcttgg aaggtgatac taggggggat gagcaacacc tttcttggag 8100
gtttgaattc ttcagggccc ctccagcatc ttggtcttcc taattcctcc cttgatcccc 8160
cagccctagg ggtggtagct tcttgcagtg tttacctccg tgttaatctg gattctctct 8220
gtcttcggag ttctctaatg cctggttacc aattctttat atcaagttct ctccgttaaa 8280
atcattgtgg tggggtccat cccatggata tacatggagg tctgtgtgac aatgtataat 8340
cttactattt gcagttatta caaaactgtc ctagaagaca tgaatgcttg ttaaaagaaa 8400
tcttttaagt ttcctagact tcaaagaccg ttgctatgcc ctgtgaaaca tcgcctatat 8460
cctttaacag tttgcaattc gaattggtta cttacttaac attttgttgc cctggtgata 8520
caattatgtt gactcgttat tgtgaggtta ctttgttatt aaccatgatg aggattacta 8580
gcacatactt ttactccata tgtataacac aacaatgtag taaaaccaat aactcttggt 8640
tctctattca aagtgtttgg aatctttatt ctgtttaaaa cactgatgac atttgccaat 8700
ttttgagtgt tcttcatgtt actgatttgt aggagctctt tatatattgc agatttagtc 8760
tcttactggt tatgtatgct gcacctgagg agtagcagca ctgccaagga cactgagtgt 8820
cttgtctgtg cagagcttca catgaagctg gagagaaggg actggggcaa gagggaaaga 8880
taaaaagggg caagtaagta acagaatgaa tccttgttgc taatctcctg gcccccatcc 8940
cagacccttc aggggatcct ggcgattcga agcagcatag cctgataggc ctctggtgag 9000
ggtgttggga gggttttctt ggccgcctgt tccaggatgc tcaagatgat gctgtgggcc 9060
tcctggcata gctcagcacc caggaaagcc tccactcgtc cacgccatgc tgccagtcgt 9120
ggccgtccct caaacagttc atagccgaga gccaccggct gtagggacaa ggatgaaaaa 9180
gttgtgggca tacaactttc ccagttgtca ctgccgaccc gaatgctgga accatgggca 9240
gaagtatcag agacatggtc cattccccag cccctaagtg aatggaccac aggggcccag 9300
gcagtatgtt cagccttaga gcccaagagc agccagggtg agaccccaca cccaggcagg 9360
aggcagaagt tcagggttct ggccccagat catccagaga cttgctctat gattctgggc 9420
aaagttctgg ctttctccag gcctcagtgt ttctgtctgc ctcatgggtg tgacacgaag 9480
agggacactg cccacaggct gagctcacac ctgcatcagc tcctccaggg ccatgagatc 9540
agccagtgtc acctgctggc cagcgaggaa gggcctgtcc cccaggaact tgtcctccag 9600
ccattgcagg gcctggtcca tggcagtcct gttgcgttcc accttctcct cgggcacctg 9660
gaccccaatg aggggcccca acacctgatg ggggcagaga gtgggtcagt ctatggcccc 9720
ggcctactgc caactactcc ctgatggcca atcactctcc agatggctct cctcacctgg 9780
acccacaggg gtataccaaa ggtgccacgg atgcagtcgg catgccagcc caggtactca 9840
tgaacacggg cacgagcctg caggtcagat ggataccagt ggtccggcgt ctggtactta 9900
cagctcaggt aaatcaggat ggccgagctg ggaacaaatg ggcagtggct ataaggacac 9960
tggcaccagg catttacccc taactgctcc catctgccag tggggcccgg gggcttctct 10020
gcatttgaag gactgccctc cagcctcgcc ctccatctcc agctccccaa caccaccccc 10080
tcctcctacg agtctcctcc tgtctttgtt gccttgggca ccgactcttg gctggacctt 10140
ctgctttgca ttttgagaac ctcatctctg tgatatgcca gcagccgccc tgtgaggtag 10200
acactcctgt tagcctttta gcacaagaga aaactcaagg tcagaatggt caagtgactt 10260
gtccgaggcg gtgcagctca gaataggctc cctggacccc tggaaccctg catggggctg 10320
gcacacagtg gatgctccgt ggagtccctt gcatgtccac gcgcttgggt cagggatcac 10380
gaggggagga atgtctttgg cccccgtctg acaggccccc agccatcact tctgcagcca 10440
cctgacctct tgtccttttc ctgcccagca gctggacctc accatgagct gtgcccttgg 10500
gccatggcat tggcctctag gcccggcctg ggtagattgg gtagtctgcg acaagtcact 10560
agaggctctt ttcagctagc atttgttgaa caaatgcgca acagtggaaa aatgttccct 10620
tgtcttctct tctaataccc ttcagtctgg gagtggagag gccctgcggc tcagaaaagg 10680
ctggaagatg agaggtgggg ggacatgttc tgtcagcccc tgctcatccc ggtcacaccc 10740
caagctgtac cttctcccca gatacctctc ctttctttct ttcctttttt tttgagacgg 10800
agtctccctc tgtcgcccag gctggagtgg gctccctctg tcacccaggc tggagtgcag 10860
tggtgctatc tctgctcatt gcaagctccg cctcctgggt tcacgccatt ctcctgcctc 10920
agcctcccca gtagctggga ctacaggcgg ccgccaccac gccctgctaa tgttttgtat 10980
ttttagtaga gacagggttt caccgtgtta cccaggatgg tctcgatctc ctgacctcgt 11040
gatccgccca cctcggcctc ccaaagtgct gggattacag gcgtaaacca ccgcgcccgg 11100
cctttttgag acggagtcta gctctgtcgc ccaggctgga gtgcagtggc gcgatctcag 11160
cacactgcaa gctccgcctc ctgggttcac accattctcc tgcctcagcc tccagagtag 11220
ctgggactac aggcgcccgc caccacgccc ggttaacttt ttttccattt ttagtagaga 11280
cggtgtttca cggtgttagc caggatggtc tcggtctcct gacctcgtga tccgcccgcc 11340
ttggcctccc gaagtgccgg gatgacaggc gtgagccacc gcgcccggcc agatccctcg 11400
cctttcccct ccctcgctgc cccatggggt atgggagggc cactggggcc cggggccagt 11460
ggggagagcc aaggtcacat gggctccgga tgcggtgagg ggtgagggaa ggagggcacc 11520
tttcagtcaa gatgaaatca ccatccttga gcgtcggcag tttccccagg ctgttgatct 11580
gcaagaactc cttgctcttg tgctgccctg aagaggaaga agtcagaaaa ggtcttcaga 11640
ataaaaacgc tgcacctcta caccctccct cctcctcccc aagcggagcc ccacagccct 11700
gagaaacagc aaggtctggg actagaggcc tggatcagcc tcactccctg gctgggcctc 11760
cctgccctgc cacattccgg ggcagctggg gggttttggt gggtggggcc aggtgcagca 11820
ggtggataaa gagaagtttt ctagccggac tctggtgggc caggggaggg gaggcaaact 11880
ggggtggctc atctcttccc atctcttaat tctcacaaca gtatgtcctt cttcccgagg 11940
tcttctgggc tttgtgaatt tatatgcgtg cattccacgc aagaactctg tcaactccat 12000
tcagtatttt ctttccatcc ctacagagca gaaggtaacg ttatcctcct tttcagaagg 12060
caagctaagg ttcagagagg ctgtgatccc ttcaaggcca ctcagtccac agcattctct 12120
ggtggcagac gctgcaggag gaggtgaggg ggctgtgcgg gcggcgagac gctcagggca 12180
gagcagggcg ggggcctggg ggtgcactgg gtttgtggac acgcggggaa acgggctggg 12240
cccacctttg accaaatcca cggtgcgcag ctctaagggg atgccattct tcttggcgaa 12300
gatgtagacg gcgcggctgg gctgggacac caggtcaaga aacagctcta ggcccatggc 12360
gggggcggca aggacagcgg ggatggcagt gaaggcgctg agcgcggtgt gggcagcagc 12420
tgtggcagga tcccggcgcg ccgggaaata ggggatcagg ccccaccccc tggggacagc 12480
acccaattgg agcgcaccac ccccggaccc gcctcgcccc ttcggttcct gtccagtcct 12540
gccggccaag actccaccac cagatccgtg cgtccctaca gggagggcgg tcgctatgaa 12600
cgcacagctg ggagggtgga gttggagctg gggaccctcg actggcaggg aggacgcgga 12660
tgcagggggc cgactgcaag gggaagggga accggctgga cagggagaag caggtctgct 12720
tttcgggatc ccggtgccag ggaccctgcc cagttccagg cgtcgccctg acccagaaac 12780
gactgggcgc cgccgtcctg gaaaggcccc agcgcacgga catctgaggg ttcgttcaga 12840
gctctgtttc tcggcgctgc atggtggcgg aagggaggga gcgaatggga tcccctaaaa 12900
gggatcttag agtttcaccc agtgggatgt gacacttgca ggtgtcccaa actggtggga 12960
accttgactg gaaggctggg gttaaggatg aactttctgc cgtccagact gtcccctgca 13020
gagcagctgc tgccagacag cgggagctgc cagacagcgg gagctcccac ttcgtgcaca 13080
ggatgggggc agggagcccg cagccgcggg aggcaggaat gactgtccgg gaacctcctt 13140
tcttctccct gaatcccagc cctggcatct caccaggggg cacagtgatg gtccagggct 13200
gggcccggga ctctagctga atctttcaga gtatcccatc cctctggcca gtggcccaag 13260
cgagtgaacc agaatgcttc cttgggagtt ttgaaactgg aactggagag aggagctccc 13320
tatggggagg taaacgggag ctggggccac ctgtagtgac atttcctgag ttcgaggagt 13380
agacgagact gagagagaaa agctgactca gagaaaggga gtgataacag ggcatgctgg 13440
cccacacctg caatcccagt tactctcacg ggatctgttt ctctgatgtc tgggtatgaa 13500
aggactttct aagcctcaga acagtgggag aactcaacaa agaaaaaacc aatacatata 13560
caaggttact ttgtggagga aaaaatggat taaccttaag caaaaaactg ggaaggatct 13620
cggtaaaaga tacgtcagaa ggaagtaatg tcctttaaga tgttcttaga aacccatcag 13680
acaggtgggg tgtggtggtt cacgcctgta atccctgtac tttgggaggc agagatgggc 13740
ggatcagttg aggtcaggag tttgagacca gcctgggcaa cacggtgaag ccccgtctct 13800
actaaaaata caaaaattag ctgggtgcgg tggcacactc gggaggctga gacaggagaa 13860
tcacttgaac cttggaggca gaggtttcag tgagctgaga tcataccact gcactccagc 13920
cgggccactg agcgagactg tctcaaaaca aacaaacgaa caaacaaaaa gaagagaaac 13980
tcatcagacg aagacacagg aaaaaaatga gcgaaggaaa tcagcagagg tttcattgaa 14040
ggacaaagag aaatggtcaa tacatggatg aaaacatgtt taacttcagt aataatcaag 14100
gaagcacaca ccaacacaac atgcacatac tgtttttatt tatcaaaggc acacatattt 14160
ttgaaatgag tactcctaat taatatgtac agagcactta cccagtgccc agcacagggg 14220
tggcaccctg tgtgtgagac agcatgaaac aggtagacac gcgccctgct gaaagtaagg 14280
gaccgcctct ctggaggatc catcgggcaa taaggaggtt tccacacctt aactgtgtct 14340
gccttgacct ctggggcctg ggagcagagc cccctccagc tggtggggaa ggaagcgtgg 14400
tgtgtttgag gacagaatgg agagaagttg agtagagcaa gtgtagactc ttcctatcca 14460
aagtgtggtc aatggactgg gagtatcagc atcaccaggg agcttgttgg aaatgcagag 14520
gctcaggccc cactctgacc ttactgattg ggagcataaa ctgtaaccag attccaggga 14580
ggattcatac acacattccc gtttgataag tggggggtag taggaggtga ggtgaaaagt 14640
ggggagggga tggctaccgc actggagaag gctggctttg tgttgaggcc tttctcatga 14700
gggcagtggg gagccatgga aggctttatg caagagaggg tacgggcaga ttgagattac 14760
agaaggatcc cactggttgc cttgtgtgag gacccaggga gaagggggag gctgtccctg 14820
tttgagtagg agatgagagc tactggtggc tatgagtgga aagaagtgag cagaggtgag 14880
cagaaatccc aaggactggt gatgatagga tgatggtggg aaccaggatg ggctttgggc 14940
agaccctgac ccctctctct gcctgtcggc ccaccagata ataatccctg tgttcctggg 15000
cgagtcagtg ccacccgaga tgttggcggc cactttggct gagctggacg gatgcctgca 15060
gctgctcgag gacaagttcc tgcgggacca ggccttcctt actgggcccc gtatctctgt 15120
ggctggcttg gtggcaatca cggagctgag gcatgagagt gccatggggt gcggtggccc 15180
gctgggcagt ggtgtatccg ggaagggagc tgacatccca gctcatgttg tcttttctgg 15240
ctgtgggacc ctgtgtgagt cacttctcct tctgagcctc agtgtcctca tctataaaat 15300
ggggctttac aaacccctca ccgcagctat attaagaggc ttccaagtgt ccccagggag 15360
ggggacatcc tagcccatca cacacatggt ggaggaggga aaatccaatc agagaacccc 15420
taaagcaggt catgctgcct tacacttggc tatgcccagc ccctccgctg actctgtctt 15480
cccctagccc atcagtgctg gctgctgagt ctttgaaagc ggacccacgg tggcagcatg 15540
gtgcccaaca cgtggaggct gcagtgcggg aggacctctt ccaggaggcc ctcccagctg 15600
tcctgaaggc caaggacctg cctccagtag aacctgctgt taaagagaat ctgaagacct 15660
taatgcagct tttcttgctg tgagtgcgtg tcccacactt gctgagccac tgaggggatg 15720
ctgtgttggt agaataaaga catggagctg tccgtctcct tggttgaaga gaagacatat 15780
ctgcaaaggc tctggtccac agttcctcca gatatcatgc ctgcattcct tttgtctcct 15840
accccattcc attctagctt ccatgtgacc tctgaaaaga gcttgggcaa acgcttactt 15900
gaccctgacc ttctgtgtgg aactttgtat ggttcctcac tgcccagaag ctaaagtaca 15960
agtcattgaa ctttgcattc aaggccttgc tcccactcct ccaggtggcc ccttctgcct 16020
gcacactcct aacccctctc tgggctccca ccttggcctc ttggcctccg ctcatgctgt 16080
tccctcctca tcttcgcctg gtgcccttcc tggtcttttg gcatttggca ccctgtctct 16140
tctccaggga gacttccctg acctccccag ccccagtcca ggtcaggcga cctctctggg 16200
ctcctcagcc ccagtgtttc cttgcggggg atacccccgg actgagcatg tatcatcgat 16260
gaggggtgac ctggtggaaa gatgtctgaa tcaggatcag ccttggtctc ctcggtctct 16320
ctcctcactg tggggcgatg ttgtgttcca caagtggggt gcagggctgg ttcatgaacc 16380
tccttgcagg aagggaagtc cctggttatt tcctggccct ctcccatgcc tcctcataac 16440
cttgaccaca gtcttctcta caccccactc ccggaccact ggcttgtcca tgccccacac 16500
ctggtcccag acttgccacc tgtccctgcc ctctcccctt aggactgtca tatttacctg 16560
tcgtgtgtct cagaccttct gatatctgag tcatctaata aacaaactgc taataggaca 16620
agatcatgac agacagagac tctgtcggcc tttcagtgga ggctccttta agtatgcaca 16680
cttatagaga attttataca cgtagttgaa attgtacata taaacttgca aattttttct 16740
ttcttttttg tgggggaggg gggagcctgg gtctcactct gtcatctagg gtggagtgca 16800
gtggcacagt catagctccc tgcagcctag atctcctgcg ctcaagcaat cctcctgcct 16860
cagccgccca ggtagctagg actacaggca cacaccacag cacccagatt tctttctttt 16920
tttttttttt ttttgtagaa aataaagaag agaatcttgc tatgttgcct agcctgatct 16980
ttaactcctg ggctcaagca atcctccctt cttagcctcc caaagtgctg agattacagg 17040
tgtgagccac attgctcgac cacaacttca gtcatatttg taatgtacaa ttcaagtgat 17100
tttgccacac tgaccacact catgtttaag ccacgccaac agagttccat gttttctgat 17160
gttttttcga gatggagtct cgctctgtca cccaggctgg agtgcaatgg cgcgatctcg 17220
gctcactgca acctccacct ccccggttca agcgattctc ctgcctcagc ctcccgagca 17280
gctgggatta caacgtctgc caccacgccc ggctaatttt tgtatcttta gtagagacgg 17340
ggtttcgtca tgttggccag gctggtctca aactcctgac ctcaggtgat ccgcccgcct 17400
ggacctcgca aagtgctggg attacaggcg tgagctaccg tgcccggcct ttccatgttt 17460
taaagaacat attttgccac cccctggtgg acagtggctc accaccggca caagaggcta 17520
cacaggcaga tgtcaatggg gaccaggcag ggacaggtat tgtcgtgagc ctagccctac 17580
ccgcgccccc gcgagtaacc acatctcctg actgcccaag cgcagatttc catactgaac 17640
atgaaattgc ctgacttcga aatggtggca aatcattcaa aaaaacttta agctcccgtt 17700
gtattggtta ttagggtcga gcctggggaa gacccctata ggtgtgtgtg tgtccttgtg 17760
tgtcgggggt gtggtgttca gacctctaat agggctagga accgggcgac cacagcgcgg 17820
aagcttgaga gggaaaccca cctggcgcca ggcaggaggg tcgggggaga cagggtgggt 17880
ccactaccgg gttaaagacc tgtagtgggt ggggctacac gtagggcgga gacgatggga 17940
cttccggaaa tcagccggca cacgtgactt ttgtttgcag aagcgggagg taccctaggc 18000
agccaatcgg ggagcgccga gtctctgtcc agccaatgag aagccaggtt gctgtggcgc 18060
ctcgcccctc ctccctggtc cgcgagcctt gggtaccccc agcttttctt ccgccagagc 18120
tgtttccgtt cctctgcccg ccatgccgtt cctggagctg gacacgaatt tgcccgccaa 18180
ccgagtgccc gcggggctgg agaaacgact ctgcgccgcc gctgcctcca tcctgggcaa 18240
acctgcggac gtaagcgtgg gccgggcagc acggggcgag gggaggttgg tgggccaggg 18300
gtccggccct gtccctgctc cgcctccccg acagtgaccc cgaatctttt ccccagggac 18360
cactccccac tcctttcctc acgccaagct ctgactttcc gtgctccacg atcccgcggc 18420
tccccctccg cacgtctttc ccttgtcgcc ctccccagtc atgacccggg cgtgaccttc 18480
agggaccgcg gcccgtatcg ggatccctgc cccgcgaaca ctgcgcgttt cggctttcgc 18540
gcgctcgggt cccgtcccca gaggtagccc ggccggctcc aacttcgggc aaaacttttc 18600
atgtccccct cagcgcgtga acgtgacggt acggccgggc ctggccatgg cgctgagcgg 18660
gtccaccgag ccctgcgcgc agctgtccat ctcctccatc ggcgtagtgg gcaccgccga 18720
ggacaaccgc agccacagcg cccacttctt tgagtttctc accaaggagc tagccctggg 18780
ccaggaccgg tgcgtagggg tagtagggga tccatgtggg actgccgcag actggagcca 18840
ctgatcctgc ctcaggggga aaaacccatt tcttgccctg cccagtaagg acacatcagg 18900
gtctggagct ttggggcccc ctgacccctt aggttcctgc tgttaggacc atcttcaaag 18960
tgcgagcagg attgaatgaa tttctggctc tgctcctcag tgtgtaagtc tgtgaaccgg 19020
gaaggctctc ttttaacacc cccggggcag tgcaagggtc atgtgggatt gtctgtgtgc 19080
tgtacctgcc ttggcacctg acagggtagg tacacgtggc tgaagtgtga ttttctagaa 19140
cttttccagg ctggtcagaa ggaattctgg gtatgttctg aagttacgta ttttggacct 19200
gtgtcccagc caggttccag gtgaagttca cgggagactc acagagtagt gaaagaccat 19260
tggcctggat gtctagacat ctgctttctg ggtcctgcat agctggggga ccccagacaa 19320
acttggaaat gaaccatctc cagttggcaa cctcctcttc tgtgaataca ggggaaaaga 19380
cctccctccc ccacaagaag cgtctgcaac ccaaacctgg cgttctgtga ccgagttaaa 19440
gtttcctctt gggtaaaaga tattcttgag ccacatccat gtctaggagg aagtaagggc 19500
atgagaagct tgaaaggaca ctgtccaggc acggtggctc atgcctgtaa tcccagcact 19560
ttgggagacc aaggcgggag gttcatttga cccaggagtt gtagaccagt ctgggtaaca 19620
tagtgagatg ccatccccca aaacagtttt aaaaattaac cggacatggt ggtgtgcaca 19680
tgtagtctca cttagttggc aggctgaagt gggaggatgg tttcagccca ggaggttgag 19740
gctgcagtga gctatgattg cgcattgcac tccatcctgt gtgacagtga gaccttggct 19800
cacaaaaaaa gaccttctga aatggagcct ttgttagtcc atgaaggtcg atgaggaatg 19860
gctgatcctg ctgggtcctc cctcaagcta cagaggaata atacagtcag ccccctgtat 19920
cactgggtta cgcatctgtt gattcaaaca accatggatt gaaatattag aggaaaaaaa 19980
attgataatt gcatctatac tgaaaatacg tatagacttt attccttgtc attattccct 20040
aaacaataca actatttaca tagtacgtac attatattag gtattataag taacccagag 20100
attatttaaa gtatatgaga ggatgtgtgt aggttacatg caaatattac accattttat 20160
gtaagggact tgagcaaatg tagattctgg tttcctctgg ggattctaga acaaatctcc 20220
cacggatact cagggaaaac tgttactcta acaacaagtg ttatacactt accatgtgct 20280
aggtcctcta caggtacttt acactcatga tcccatttga tccttacaat ccctatccac 20340
tctccctttg ctcagacaag acatgctatc cccatgaggt agataatctc cattatgcca 20400
attttatgat gagaagacta aggctcttca tactcccccg ccctcagcca ggctgtccca 20460
agctgtggcg ctcctcactg aaaactgctg tcacctctga gaggggcatg ttggcttatg 20520
gcacagacat tagtggtggg ggggccaccc tgtgcccaac ctttgagaaa acaggtgtct 20580
gaggtactgt gtccttaggg agtctgcaat taggaggagg cagattgccc ctcagcccac 20640
agactgacag agatgatgga gtgtgcagca cacacgatga ggctactgtg ttgagtgtct 20700
gtcctaggtg ggtcatgctg atgtgcagcc accattccac acctgagtgt cccactgccc 20760
tgctgggggt tggggaatgc tcattaccgg gatgagcact tttgcaaaat gggctgaggt 20820
gggaggatca cttaagccca ggaattcaag accaccctgg gaaacaaagt gcgaccccat 20880
ctctacaaag tcatacaatt agcccagcat ggtggcatga gcctgtgatc caaactacat 20940
gggaggctga ggcagaaggt ttgtttaggc ccaggaggtc gaggctgcaa taatctgagt 21000
ttccaccact gcactcactc catgctggct gcctttggat tgttcctaat tggatggttt 21060
tgctttcaac agacactcca ataaagtaca cgaaataaag aataaagtta aacagtcagt 21120
cccagtgctg tgtaggtggc agtaagtctg ggagagcgac ctcggctgaa ctaactgtgg 21180
tgtgcatcat tgttaagcag ggccagcagg atcctcatgt cacctggcag gaggagggac 21240
agggaagggt ccctagaagt gaccttgagt tgcacttagt gctcttggca gagggaaggg 21300
cttgggctag tgctggaggc atgaagatgg ggtgtgttca gggctctcca gcagtccagg 21360
catgggaagg ggacgctgag ggccaaaggc ggaggtgaca ctgggaaggg aactagcaag 21420
gtgaggactg gcttttggcc atgtgactga attgcctgtg tggtgcgtgt gtatgtgtgc 21480
gaatgtcagt gaactgtgtg tgagtgaatt gtgtgtttat gtgtgggtgt atatgtgtgt 21540
gatgtgagtg aactgtgtac atgggtgtat acgtgaattt tagtgaattg tgtgtaaatg 21600
tgagcagtgt gaatttgagt atgtgggtgg ataagtgaat tgtgattttg ttggtgtgaa 21660
ttgtgtgcct gtgtgtgacg gggagtatgt gtgtgtgtga gtgaactgtg tgatgactat 21720
gaatgtcagt gaatgctgtg attgtgtgtg tgtgtgtacg tgtgtgggga tttggtctat 21780
atgtgtgaat ttgagttgtg tgactttgtt ggatgtgagt gaattatgtg actgggtgta 21840
tatgtgtgtg tacacgagtt aactgtgtgt aaatggtatg attgtatatg tgtgtgaatg 21900
tgatgaatcg tgactagatg cgggtggatg tgtgtatgtg catggtgtgt ccatgagtgt 21960
gtaagtgtgc aggtggcaca gttgcaacag gagagaagac acaggaagcc ttacctgcca 22020
ttcatccccc cagctgtcct cccagcctgg ggacccctgg gctggaaagg ttatgaaaag 22080
tggtgctact gccaggaagg ggtgatgggg cagctgggag gacggtttga gtcttgccaa 22140
agggagggag tcgaagcttg caaccctggg gaggaggagg ggcagactgg ggaccctgct 22200
tctggaaaac cagcgaccaa cccccaaagg gacacaggca acccctgcct ccactctaca 22260
gtggggaaaa ggggagagtg gcgtggcagg ctgcccagcc agtcatggag ggagtagact 22320
tcaggaggag tacagaggtc tggtcccatt ttggggctga ggacaggagc ctcaggtcag 22380
cagtctgcag gaaggcccca gctggaccag ctgctcacac cttcccacag gcctgcagat 22440
gggtgtggag agcagagcct ccaggctgag tctcccagcc ccagatctga gcagtctaaa 22500
tcatccccct ccaggttccc tacggtctta tccaccagcc ctgctgccca tggtggcccc 22560
agatgcccag gagagataat agaaggtaag aagtcatgtt tgaatgagga agctctcttc 22620
atttatttca tatgaggatg aagaagagga ttatgtgatc acaggaatgt tgcatgcggg 22680
ataatccaaa gctggttatc tccaggccct cactctgcca agagatctct ctggaagaag 22740
cagccagttc acagatgccc tggatccctc cgtgcccaat cataaaaaag tcatgaccgt 22800
ccctatcttg ccaatctgcc aggactccaa ggggaaaaag cggataagta tccttcagga 22860
gacagagaaa aagatatcat cagctccttg gctaatacca catcttgcaa gacccctgcc 22920
aggtactccc actgtgggta ctcaggacag cctgcctcag tccaccaggc attttgcaaa 22980
cctgctcatc ccaataatga ttttccccaa cccccagcag ggcagtggga cactcaggtg 23040
tgggaggtag ccgcacatca tgacccagct aggacagaca cacaatacag tagcctcggt 23100
gtgtgtgccg tacactctat catctccatc agtttgtgta ctgaggggta ccctgcctcc 23160
taattgcagg ctccctaagg gcacagtacc tcagccacct gttttctcaa aggctgagca 23220
cagggtgacc ccccacccca ccaaccccgc cactaatgtc tgggccataa gtgaacatgc 23280
ccctctcaga ggtaacagca agtttccagt gaggaaaacc agaacttggg acagcctggt 23340
tggggcgggc gggagtatga acagcctgtc ttctcatcat aaaattggca taatggagat 23400
tatctacctc atggggatag catgtcttgt ctgagcaaag ggagagtgga tagggattgt 23460
aaggatcaaa tgggatcatg agtgtaaagt acctgtagag gacctagcac atggtaagtg 23520
tataacactt gttgttagag taacagtttt ccctgagtat ccgtgggaga tttgttctag 23580
aatccccaga ggaaaccaga atctacattt gctcaagtcc cttacataaa atggtgtaat 23640
atttgcatgt aacctacaca catcctctca tatactttaa ataatctctg ggttacttat 23700
aatacctaat ataatgtacg tactatgtaa atagttgtat tgtttaggga ataatgacaa 23760
ggaataaagt ctatacgtat tttcagtata gatgcaatta tcaatttttt ttcctctaat 23820
atttcaatcc atggttgttt gaatcaacag atgcgtaacc cagtgataca gggggctgac 23880
tgtattattc ctctgtagct tgagggagga cccagcagga tcagccattc ctcatcgacc 23940
ttcatggact aacaaaggct ccatttcaga aggtcttttt ttgtgagcca aggtctcact 24000
gtcacacagg atggagtgca atgcgcaatc atagctcact gcagcctcaa cctcctgggc 24060
tgaaaccatc ctcccacttc agcctgccaa ctaagtgaga ctacatgtgc acaccaccat 24120
gtccggttaa tttttaaaac tgttttgggg gatggcatct cactatgtta cccagactgg 24180
tctacaactc ctgggtcaaa tgaacctccc gccttggtct cccaaagtgc tgggattaca 24240
ggcatgagcc accgtgcctg gacagtgtcc tttcaagctt ctcatgccct tacttcctcc 24300
tagacatgga tgtggctcaa gaatatcttt tacccaagag gaaactttaa ctcggtcaca 24360
gaacgccagg tttgggttgc agacgcttct tgtgggggag ggaggtcttt tcccctgtat 24420
tcacagaaga ggaggttgcc aactggagat ggttcatttc caagtttgtc tggggtcccc 24480
cagctatgca ggacccagaa agcagatgtc tagacatcca ggccaatggt ctttcactac 24540
tctgtgagtc tcccgtgaac ttcacctgga acctggctgg gacacaggtc caaaatacgt 24600
aacttcagaa catacccaga attccttctg accagcctgg aaaagttcta gaaaatcaca 24660
cttcagccac gtgtacctac cctgtcaggt gccaaggcag gtacagcaca cagacaatcc 24720
cacatgaccc ttgcactgcc ccgggggtgt taaaagagag ccttcccggt tcacagactt 24780
acacactgag gagcagagcc agaaattcat tcaatcctgc tcgcactttg aagatggtcc 24840
taacagcagg aacctaaggg gtcagggggc cccaaagctc cagaccctga tgtgtcctta 24900
ctgggcaggg caagaaatgg gtttttcccc ctgaggcagg atcagtggct ccagtctgcg 24960
gcagtcccac atggatcccc tactacccct acgcaccggt cctggcccag ggctagctcc 25020
ttggtgagaa actcaaagaa gtgggcgctg tggctgcggt tgtcctcggc ggtgcccact 25080
acgccgatgg aggagatgga cagctgcgcg cagggctcgg tggacccgct cagcgccatg 25140
gccaggcccg gccgtaccgt cacgttcacg cgctgagggg gacatgaaaa gttttgcccg 25200
aagttggagc cggccgggct acctctgggg acgggacccg agcgcgcgaa agccgaaacg 25260
cgcagtgttc gcggggcagg gatcccgata cgggccgcgg tccctgaagg tcacgcccgg 25320
gtcatgactg gggagggcga caagggaaag acgtgcggag ggggagccgc gggatcgtgg 25380
agcacggaaa gtcagagctt ggcgtgagga aaggagtggg gagtggtccc tggggaaaag 25440
attcggggtc actgtcgggg aggcggagca gggacagggc cggacccctg gcccaccaac 25500
ctcccctcgc cccgtgctgc ccggcccacg cttacgtccg caggtttgcc caggatggag 25560
gcagcggcgg cgcagagtcg tttctccagc cccgcgggca ctcggttggc gggcaaattc 25620
gtgtccagct ccaggaacgg catggcgggc agaggaacgg aaacagctct ggcggaagaa 25680
aagctggggg tacccaaggc tcgcggacca gggaggaggg gcgaggcgcc acagcaacct 25740
ggcttctcat tggctggaca gagactcggc gctccccgat tggctgccta gggtacctcc 25800
cgcttctgca aacaaaagtc acgtgtgccg gctgatttcc ggaagtccca tcgtctccgc 25860
cctacgtgta gccccaccca ctacaggtct ttaacccggt agtggaccca ccctgtctcc 25920
cccgaccctc ctgcctggcg ccaggtgggt ttccctctca agcttccgcg ctgtggtcgc 25980
ccggttccta gccctattag aggtctgaac accacacccc cgacacacaa ggacacacac 26040
acacctatag gggtcttccc caggctcgac cctaataacc aatacaacgg gagcttaaag 26100
tttttttgaa tgatttgcca ccatttcgaa gtcaggcaat ttcatgttca gtatggaaat 26160
ctgcgcttgg gcagtcagga gatgtggtta ctcgcggggg cgcgggtagg gctaggctca 26220
cgacaatacc tgtccctgcc tggtccccat tgacatctgc ctgtgtagcc tcttgtgccg 26280
gtggtgagcc actgtccacc agggggtggc aaaatatgtt ctttaaaaca tggaaaggcc 26340
gggcacggta gctcacgcct gtaatcccag cactttgcga ggtccaggcg ggcggatcac 26400
ctgaggtcag gagtttgaga ccagcctggc caacatgacg aaaccccgtc tctactaaag 26460
atacaaaaat tagccgggcg tggtggcaga cgttgtaatc ccagctgctc gggaggctga 26520
ggcaggagaa tcgcttgaac cggggaggtg gaggttgcag tgagccgaga tcgcgccatt 26580
gcactccagc ctgggtgaca gagcgagact ccatctcgaa aaaacatcag aaaacatgga 26640
actctgttgg cgtggcttaa acatgagtgt ggtcagtgtg gcaaaatcac ttgaattgta 26700
cattacaaat atgactgaag ttgtggtcga gcaatgtggc tcacacctgt aatctcagca 26760
ctttgggagg ctaagaaggg aggattgctt gagcccagga gttaaagatc aggctaggca 26820
acatagcaag attctcttct ttattttcta caaaaaaaaa aaaaaaaaga aagaaatctg 26880
ggtgctgtgg tgtgtgcctg tagtcctagc tacctgggcg gctgaggcag gaggattgct 26940
tgagcgcagg agatctaggc tgcagggagc tatgactgtg ccactgcact ccaccctaga 27000
tgacagagtg agacccaggc tcccccctcc cccacaaaaa agaaagaaaa aatttgcaag 27060
tttatatgta caatttcaac tacgtgtata aaattctcta taagtgtgca tacttaaagg 27120
agcctccact gaaaggccga cagagtctct gtctgtcatg atcttgtcct attagcagtt 27180
tgtttattag atgactcaga tatcagaagg tctgagacac acgacaggta aatatgacag 27240
tcctaagggg agagggcagg gacaggtggc aagtctggga ccaggtgtgg ggcatggaca 27300
agccagtggt ccgggagtgg ggtgtagaga agactgtggt caaggttatg aggaggcatg 27360
ggagagggcc aggaaataac cagggacttc ccttcctgca aggaggttca tgaaccagcc 27420
ctgcacccca cttgtggaac acaacatcgc cccacagtga ggagagagac cgaggagacc 27480
aaggctgatc ctgattcaga catctttcca ccaggtcacc cctcatcgat gatacatgct 27540
cagtccgggg gtatcccccg caaggaaaca ctggggctga ggagcccaga gaggtcgcct 27600
gacctggact ggggctgggg aggtcaggga agtctccctg gagaagagac agggtgccaa 27660
atgccaaaag accaggaagg gcaccaggcg aagatgagga gggaacagca tgagcggagg 27720
ccaagaggcc aaggtgggag cccagagagg ggttaggagt gtgcaggcag aaggggccac 27780
ctggaggagt gggagcaagg ccttgaatgc aaagttcaat gacttgtact ttagcttctg 27840
ggcagtgagg aaccatacaa agttccacac agaaggtcag ggtcaagtaa gcgtttgccc 27900
aagctctttt cagaggtcac atggaagcta gaatggaatg gggtaggaga caaaaggaat 27960
gcaggcatga tatctggagg aactgtggac cagagccttt gcagatatgt cttctcttca 28020
accaaggaga cggacagctc catgtcttta ttctaccaac acagcatccc ctcagtggct 28080
cagcaagtgt gggacacgca ctcacagcaa gaaaagctgc attaaggtct tcagattctc 28140
tttaacagca ggttctactg gaggcaggtc cttggccttc aggacagctg ggagggcctc 28200
ctggaagagg tcctcccgca ctgcagcctc cacgtgttgg gcaccatgct gccaccgtgg 28260
gtccgctttc aaagactcag cagccagcac tgatgggcta ggggaagaca gagtcagcgg 28320
aggggctggg catagccaag tgtaaggcag catgacctgc tttaggggtt ctctgattgg 28380
attttccctc ctccaccatg tgtgtgatgg gctaggatgt ccccctccct ggggacactt 28440
ggaagcctct taatatagct gcggtgaggg gtttgtaaag ccccatttta tagatgagga 28500
cactgaggct cagaaggaga agtgactcac acagggtccc acagccagaa aagacaacat 28560
gagctgggat gtcagctccc ttcccggata caccactgcc cagcgggcca ccgcacccca 28620
tggcactctc atgcctcagc tccgtgattg ccaccaagcc agccacagag atacggggcc 28680
cagtaaggaa ggcctggtcc cgcaggaact tgtcctcgag cagctgcagg catccgtcca 28740
gctcagccaa agtggccgcc aacatctcgg gtggcactga ctcgcccagg aacacaggga 28800
ttattatctg gtgggccgac aggcagagag aggggtcagg gtctgcccaa agcccatcct 28860
ggttcccacc atcatcctat catcaccagt ccttgggatt tctgctcacc tctgctcact 28920
tctttccact catagccacc agtagctctc atctcctact caaacaggga cagcctcccc 28980
cttctccctg ggtcctcaca caaggcaacc agtgggatcc ttctgtaatc tcaatctgcc 29040
cgtaccctct cttgcataaa gccttccatg gctccccact gccctcatga gaaaggcctc 29100
aacacaaagc cagccttctc cagtgcggta gccatcccct ccccactttt cacctcacct 29160
cctactaccc cccacttatc aaacgggaat gtgtgtatga atcctccctg gaatctggtt 29220
acagtttatg ctcccaatca gtaaggtcag agtggggcct gagcctctgc atttccaaca 29280
agctccctgg tgatgctgat actcccagtc cattgaccac actttggata ggaagagtct 29340
acacttgctc tactcaactt ctctccattc tgtcctcaaa cacaccacgc ttccttcccc 29400
accagctgga gggggctctg ctcccaggcc ccagaggtca aggcagacac agttaaggtg 29460
tggaaacctc cttattgccc gatggatcct ccagagaggc ggtcccttac tttcagcagg 29520
gcgcgtgtct acctgtttca tgctgtctca cacacagggt gccacccctg tgctgggcac 29580
tgggtaagtg ctctgtacat attaattagg agtactcatt tcaaaaatat gtgtgccttt 29640
gataaataaa aacagtatgt gcatgttgtg ttggtgtgtg cttccttgat tattactgaa 29700
gttaaacatg ttttcatcca tgtattgacc atttctcttt gtccttcaat gaaacctctg 29760
ctgatttcct tcgctcattt ttttcctgtg tcttcgtctg atgagtttct cttctttttg 29820
tttgttcgtt tgtttgtttt gagacagtct cgctcagtgg cccggctgga gtgcagtggt 29880
atgatctcag ctcactgaaa cctctgcctc caaggttcaa gtgattctcc tgtctcagcc 29940
tcccgagtgt gccaccgcac ccagctaatt tttgtatttt tagtagagac ggggcttcac 30000
cgtgttgccc aggctggtct caaactcctg acctcaactg atccgcccat ctctgcctcc 30060
caaagtacag ggattacagg cgtgaaccac cacaccccac ctgtctgatg ggtttctaag 30120
aacatcttaa aggacattac ttccttctga cgtatctttt accgagatcc ttcccagttt 30180
tttgcttaag gttaatccat tttttcctcc acaaagtaac cttgtatatg tattggtttt 30240
ttctttgttg agttctccca ctgttctgag gcttagaaag tcctttcata cccagacatc 30300
agagaaacag atcccgtgag agtaactggg attgcaggtg tgggccagca tgccctgtta 30360
tcactccctt tctctgagtc agcttttctc tctcagtctc gtctactcct cgaactcagg 30420
aaatgtcact acaggtggcc ccagctcccg tttacctccc catagggagc tcctctctcc 30480
agttccagtt tcaaaactcc caaggaagca ttctggttca ctcgcttggg ccactggcca 30540
gagggatggg atactctgaa agattcagct agagtcccgg gcccagccct ggaccatcac 30600
tgtgccccct ggtgagatgc cagggctggg attcagggag aagaaaggag gttcccggac 30660
agtcattcct gcctcccgcg gctgcgggct ccctgccccc atcctgtgca cgaagtggga 30720
gctcccgctg tctggcagct cccgctgtct ggcagcagct gctctgcagg ggacagtctg 30780
gacggcagaa agttcatcct taaccccagc cttccagtca aggttcccac cagtttggga 30840
cacctgcaag tgtcacatcc cactgggtga aactctaaga tcccttttag gggatcccat 30900
tcgctccctc ccttccgcca ccatgcagcg ccgagaaaca gagctctgaa cgaaccctca 30960
gatgtccgtg cgctggggcc tttccaggac ggcggcgccc agtcgtttct gggtcagggc 31020
gacgcctgga actgggcagg gtccctggca ccgggatccc gaaaagcaga cctgcttctc 31080
cctgtccagc cggttcccct tccccttgca gtcggccccc tgcatccgcg tcctccctgc 31140
cagtcgaggg tccccagctc caactccacc ctcccagctg tgcgttcata gcgaccgccc 31200
tccctgtagg gacgcacgga tctggtggtg gagtcttggc cggcaggact ggacaggaac 31260
cgaaggggcg aggcgggtcc gggggtggtg cgctccaatt gggtgctgtc cccagggggt 31320
ggggcctgat cccctatttc ccggcgcgcc gggatcctgc cacagctgct gcccacaccg 31380
cgctcagcgc cttcactgcc atccccgctg tccttgccgc ccccgccatg ggcctagagc 31440
tgtttcttga cctggtgtcc cagcccagcc gcgccgtcta catcttcgcc aagaagaatg 31500
gcatcccctt agagctgcgc accgtggatt tggtcaaagg tgggcccagc ccgtttcccc 31560
gcgtgtccac aaacccagtg cacccccagg cccccgccct gctctgccct gagcgtctcg 31620
ccgcccgcac agccccctca cctcctcctg cagcgtctgc caccagagaa tgctgtggac 31680
tgagtggcct tgaagggatc acagcctctc tgaaccttag cttgccttct gaaaaggagg 31740
ataacgttac cttctgctct gtagggatgg aaagaaaata ctgaatggag ttgacagagt 31800
tcttgcgtgg aatgcacgca tataaattca caaagcccag aagacctcgg gaagaaggac 31860
atactgttgt gagaattaag agatgggaag agatgagcca ccccagtttg cctcccctcc 31920
cctggcccac cagagtccgg ctagaaaact tctctttatc cacctgctgc acctggcccc 31980
acccaccaaa accccccagc tgccccggaa tgtggcaggg cagggaggcc cagccaggga 32040
gtgaggctga tccaggcctc tagtcccaga ccttgctgtt tctcagggct gtggggctcc 32100
gcttggggag gaggagggag ggtgtagagg tgcagcgttt ttactctgaa gaccttttct 32160
gacttcttcc tcttcagggc agcacaagag caaggagttc ttgcagatca acagcctggg 32220
gaaactgccg acgctcaagg atggtgattt catcttgacc gaaaggtgcc ctccttccct 32280
cacccctcac cgcatccgga gcccatgtga ccttggctct ccccactggc cccgggcccc 32340
agtggccctc ccatacccca tggggcagcg agggagggga aaggcgaggg atctggccgg 32400
gcgcggtggc tcacgcctgt catcccggca cttcgggagg ccaaggcggg cggatcacga 32460
ggtcaggaga ccgagaccat cctggctaac accgtgaaac accgtctcta ctaaaaatgg 32520
aaaaaaaagt taaccgggcg tggtggcggg cgcctgtagt cccagctact ctggaggctg 32580
aggcaggaga atggtgtgaa cccaggaggc ggagcttgca gtgtgctgag atcgcgccac 32640
tgcactccag cctgggcgac agagctagac tccgtctcaa aaaggccggg cgcggtggct 32700
cacgcctgta atcccagcac tttgggaggc cgaggtgggc ggatcacgag gtcaggagat 32760
cgagaccatc ctggctaaca cggtgaaacc ctgtctctac taaaaataca aaacattagc 32820
agggcgtggt ggcggccgcc tgtagtccca gctactgggg aggctgaggc aggagaatgg 32880
cgtgaactca ggaggcggag cttgcaatga gcagaggtcg cgccactgca ctccagcctg 32940
ggtgacagag ggagcccact ccagcctggg cgacagaggg agactccgtc tcaaaaaaaa 33000
aggaaagaaa gaaaggagag gtatctgggg agaaggtaca gcttggggtg tgtccgggat 33060
gagcaggggc tgacagaaca tgtcccccca cctctcatct tcagcctttt ctgagccgca 33120
gggcctctcc actcccagac tgaagggtat tagaagagaa gacaagggaa catttttcca 33180
ctgttgcgca tttgttcaac aaatgctagc tgaaaagagc ctctagtgac ttgtcgcaga 33240
ctacccaatc tacccaggcc gggcctagag gccaatgcca tggcccaagg gcacagctca 33300
tggtgaggtc cagctgctgg gcaggaaaag gacaagaggt caggtggctg cagaggtgat 33360
ggctgggggc ctgtcagacg ggggccaaag acattcctcc cctcgtgatc cctgacccaa 33420
gcgcgtggac atgcaaggga ctccacggag catccactgt gtgccagccc catgcagggt 33480
tccaggggtc cagggagcct attctgagct gcaccgcctc ggacaagtca cttgaccatt 33540
ctgaccttga gttttctctt gtgctaaaag gctaacagga gtgtctacct cacagggcgg 33600
ctgctggcat atcacagaga tgaggttctc aaaatgcaaa gcagaaggtc cagccaagag 33660
tcggtgccca aggcaacaaa gacaggagga gactcgtagg aggagggggt ggtgttgggg 33720
agctggagat ggagggcgag gctggagggc agtccttcaa atgcagagaa gcccccgggc 33780
cccactggca gatgggagca gttaggggta aatgcctggt gccagtgtcc ttatagccac 33840
tgcccatttg ttcccagctc ggccatcctg atttacctga gctgtaagta ccagacgccg 33900
gaccactggt atccatctga cctgcaggct cgtgcccgtg ttcatgagta cctgggctgg 33960
catgccgact gcatccgtgg cacctttggt atacccctgt gggtccaggt gaggagagcc 34020
atctggagag tgattggcca tcagggagta gttggcagta ggccggggcc atagactgac 34080
ccactctctg cccccatcag gtgttggggc cactcattgg ggtccaggtg cccaaggaga 34140
aggtggaacg caacaggact gccatggacc aggccctgca atggctggag gacaagttcc 34200
tgggggacag gcccttcctc gctggccagc aggtgacact ggctgatctc atggccctgg 34260
aggagctgat gcaggtgtga gctcagcctg tgggcagtgt ccctcttcgt gtcacaccca 34320
tgaggcagac agaaacactg aggactggag aaagccagaa ctttgcccag aatcatagag 34380
caagtctctg gatgatctgg ggccagaacc ctgaacttct gcctcctgcc tgggtgtggg 34440
gtctcaccct ggctgctctt gggctctaag gctgaacata ctgcctgggc ccctgtggtc 34500
cattcactta ggggctgggg aatggaccat gtctctgata cttctgccca tggttccagc 34560
attcgggtcg gcagtgacaa ctgggaaagt tgtatgccca caactttttc atccttgtcc 34620
ctacagccgg tggctctcgg ctatgaactg tttgagggac ggccacgact ggcagcatgg 34680
cgtggacgag tggaggcttt cctgggtgct gagctatgcc aggaggccca cagcatcatc 34740
ttgagcatcc tggaacaggc ggccaagaaa accctcccaa caccctcacc agaggcctat 34800
caggctatgc tgcttcgaat cgccaggatc ccctgaaggg tctgggatgg gggccaggag 34860
attagcaaca aggattcatt ctgttactta cttgcccctt tttatctttc cctcttgccc 34920
cagtcccttc tctccagctt catgtgaagc tctgcacaga caagacactc agtgtccttg 34980
gcagtgctgc tactcctcag gtgcagcata cataaccagt aagagactaa atctgcaata 35040
tataaagagc tcctacaaat cagtaacatg aagaacactc aaaaattggc aaatgtcatc 35100
agtgttttaa acagaataaa gattccaaac actttgaata gagaaccaag agttattggt 35160
tttactacat tgttgtgtta tacatatgga gtaaaagtat gtgctagtaa tcctcatcat 35220
ggttaataac aaagtaacct cacaataacg agtcaacata attgtatcac cagggcaaca 35280
aaatgttaag taagtaacca attcgaattg caaactgtta aaggatatag gcgatgtttc 35340
acagggcata gcaacggtct ttgaagtcta ggaaacttaa aagatttctt ttaacaagca 35400
ttcatgtctt ctaggacagt tttgtaataa ctgcaaatag taagattata cattgtcaca 35460
cagacctcca tgtatatcca tgggatggac cccaccacaa tgattttaac ggagagaact 35520
tgatataaag aattggtaac caggcattag agaactccga agacagagag aatccagatt 35580
aacacggagg taaacactgc aagaagctac cacccctagg gctgggggat caagggagga 35640
attaggaaga ccaagatgct ggaggggccc tgaagaattc aaacctccaa gaaaggtgtt 35700
gctcatcccc cctagcatca ccttccaaga agggcaggtt tccccagttt gccctgaggt 35760
cttcacagga ggccatgtgg ctgtggatgc agttctcaaa tgtgacagta gatgtcactg 35820
ctgaccaata aacaggatgt tccagacaca ctctgcgcta cctgcccaca tgtcttgtgg 35880
ctttggggcc tatttcccat gcctgacctg tgcccaccat gtgccactct attgggggac 35940
ctgccccaat aatcacgtag attgtttcct atttttccta agtgtcggcc ggcttgagaa 36000
ataaagggac agagtacaaa agagagaaat tgtaaagctg tgcatccggg ggagacgtca 36060
cacgttagta ggatctgtga tgccccacaa gccacaaaaa ccagcaagtt tttattaggg 36120
attttcaaaa ggggagggag tgtgcaaata ggtgtgagtg acagacatca agtacttaac 36180
agggtaatag aatatcacaa ggcaagtgga ggcagggcga gatcacagga tcacaggacc 36240
gaggcaaaat taaaattgct aatgaagttt cgggcaccat tgtcattgat aacatcttat 36300
caggagacag ggttttgaga ccaaccggtc tcaccaaaat ttattaggcg ggaatttcct 36360
cttcctaata agcctgggag cactatggga gactggagtc tatctcacct ctgcagtctc 36420
gaccataaga gagaggccac gcccgggggg ctgtttataa gccgatacct ccaggtgcgt 36480
attctctttc tcagggacat tccatgctga gaaaaagaat tcagcgatat ttctcccatt 36540
tgcttttgaa agaagagaaa tatggctgtg ttctgcccgg ctcaccggtg gtcagagttt 36600
aaggttatct ctcttattcc ctgaacaatt gctgttatcc tcttcttttt tcaaggtgct 36660
cagatttcat attgctcaaa cacacatcct gtacaatttg tgcagttaat gcaattatta 36720
aagggtcctg aggcgacata catcttcctc agctgacagg attaagagat taaagacagg 36780
tataggaaat cataagggtg ttgattgggg aagtgataag tgtccatgaa atctttacaa 36840
tttatgttta gagattgcag taaagacagg cataagaaac tataatagta ttaatttggg 36900
gaactaataa atgtccataa aatcttcaca ctccacgttc ttctgtcatg gcttcagccg 36960
gtccctctgt ttggggtcac tgacttcctg taacaccact cctagccgga agtattccaa 37020
gtaaccattg caactgtcct gccttaaggg tgcctggtga tggcttctgt cttaggtccc 37080
attctggctc tctcttccag tgatttctca tactttgggg gacactctgg cctgctttcc 37140
tgggcctttt ccattaccac cccctctccc catcaggcac tggggatggc ggccacagcc 37200
tatgccttct tgatccatgc tggctgcctt gggattgttc cttattggat ggttttgctt 37260
tcaacagaca ctccaataaa gtacacgaaa taaagaataa agttaaacag tcagtccccg 37320
tgctgtgtag gtggcagtaa gtctgggaga gcgacctcag ctgaactaac catgctgtgc 37380
atcattgtca agcgggacca gcaggatcct taggtcacct ggcaggagga ggggcaggga 37440
agggtcccta gaagtgatct tgagttgcac ttagtgctct tggcagaggg aagggcttgg 37500
gctagtgctg gaggcatgaa gatggggtgt gttcagggct ctccagcagt ccaggcatgg 37560
gaaggggacg ctgagggcca aaggctaagg tgacactggg aagggaacca tagcaaggtg 37620
aggaatggct ttcggccatg tgactgaatt gcctgtgtgt ggtgcgtgtg tacgtgtgtg 37680
aatgtcagtg aactgtgtgt gtgaacgtga gtgaattgtg taactttgtt tatgtgtggg 37740
tgtatatgtg tgtgatgtga gtgaactgtg tgtatgtggg tgtatatgtg tgtgatgtga 37800
gtgaactgtg tgtatgtggg tgtatatgtg tgtggtgtga gtgaactgtg tgtaagtagg 37860
tgtatatgtg tgtgaatttg aatggtgtgt aaatgtgact gagcagtgtg aatttgtgta 37920
tgtgggtgta tgtgtgtgag tgaactgtgt gattttgttg gtgtgaattg tgtgcctgtg 37980
tgtgtgaggt gtgtgtgtgt gtgtgactga actgtgtgat gactttgtat taatgtcagt 38040
gaatggtgtg gttgtgtatg tgggtgtata tgtgtgtgta tgtatgtgaa ttgtgtgtgg 38100
ggttggtgtg tatgtgtgtc aatgtgagtg agttgtgtga ctgttgaata tgagtgaatt 38160
atgtgattgt gtgggtgtat atgtgtgtac aggagttaac tgtgtgactg tgtgtaaatg 38220
ttatgattgt gtgtgtgggt gtatatgtgt gaatgtgata catcgtgtga ctgtgggtgg 38280
acgtgggtgg atgtgtgtgt gagtgtgcgt ggctgtgtgt cgaacagtgt gtgagtgtaa 38340
gtgtgcaggt ggtagctgtg gaattaaggc acattagggg aatccttcct tggctctggt 38400
ccttggtttc cctcctatga aacaagttgg gtgaattagg cagtctctgg cattccagga 38460
tttggtctca ggtctaaaca ggattttgta aagtgaaagc agcatccctt gggcatcccg 38520
ggaggtgtcc ctgccctcca tggctcacct tctgccacat ggcccagcag cagctactct 38580
gcagggccat gtgctgccat gacaggtact gatccccatg ggcgcgggcc tgcaggtcct 38640
ggaggtacca gtggtcaggt gctttgtaca tgcagctcat atagagcaag ctagccacac 38700
tggggggaca tggcaggggc agaagacatg ccctgaacac tcttcagcca ttttctgggg 38760
cattttcact atccccatga tgcagaggag ggatgtgagg ctcagagagg ttaggtaact 38820
gcccagggtc acacagccct acacggggcc agattatcat ctcaactaat ggcagcaagg 38880
aagaaacaaa atgtcatgaa cttcagcagg ggctcaggga aggcttccag gaggaagggg 38940
cacccagtgt gagtctgaag aatgagagca agttgggtat ctccccaaca tatccaaagc 39000
atggacaggg ccaggagcaa ggccagcagg gagagagcct tccaggtgac atggcagcat 39060
tgctccccac cccgtcccat ctcctgttca cctatggtga agggttgtgc agtctgaccc 39120
ccagagctca tcatgcctct atgggtgttg gaccatccag tgatcccagg ctctgaatgt 39180
gaatccagct ccaccaagga ggcactatgt ggcctcggta aaggacgcca ccatgcggcc 39240
accaacctgg aggctggttt tggctttcat ttttcccacc cacctccaac ccttcagcac 39300
accctgcagt tagagtttca agaaaagtca gaaccgacca tcaccccacc aaccctgcac 39360
caccaccacc ttggtctggg gctccagcgc tgcatgccag atgactgcag tggctttctc 39420
acccctgcca gggggatcct ttatgacgta agtcagatca cagttctcct ctgctcaaaa 39480
tactccagcg actcaatccc cctgcggtaa gagccaaagc cttcccagtg tcccatgctc 39540
ccctctgtcg tgacctcctg ctcttcctgc tcactacacc caccctggcc cgttgctctc 39600
cagtctgaac cccatgcatt gtcccacctg gagccttcgc cctcactctt ccctccagcc 39660
agaacaccct tctcctcatg ccttgcacat tcctacagct ctctgctcag atgccacttc 39720
ctccagggag tcttccctca acatcctact cacacacccc caatcttacc ctgtttattt 39780
tttccatggc atccatcaac atccgatatg ttccatgttt acatgggtta ctgtcagctc 39840
ccccttctat taatggaatg tgagatccat gaggagaagg acgtagacac gttcactgct 39900
caagccccag ggcttaaatg gggtctggcc ctggtggatg tttagcaaat agccactgaa 39960
gctcgctgag tggtccctcg actctccagg gaaacagcac ttgccccaca ggatttgtgg 40020
gcaaaatggt agcactaaga tgcctggtac agccgctcct cgaaacttag cactctcctt 40080
ttttcggagg ggaaggctgg cggtgaggag gcccggaagc agactgggcc ctgtggcctt 40140
gtgcaagtcc ctcccctcct ccagcctcag tttcctcact tgtacaatgc aggaggggac 40200
ttctataacc cttgtcaaca ctgggcacca ctgcctgcct ccacccattc ttcaccctgg 40260
ggctggtgca gggcccacag tccatgatcc aagccatcaa cagtgaacag acagccgact 40320
ggccccacaa ggcccggcag cagcccacgg accggttacc tctcagccaa ggtgaagtcc 40380
ccatccttca aggcccggca ccttcctcag ggggttcacc tgggcaaagg catcgctgtg 40440
ctgctggcct gcaggagaga tcagagaggt gagtggggat aacccagagt tctctgaggt 40500
ctctgcccac ccccacccca aaatcccagc agcagctttc tgaggcccct atctgggagg 40560
catcagggaa gaggaagcct ctctgctagt cactgggtca cagaccttga ccccatcaga 40620
gggccatctt tcctgtgtcc agctccttga agcatggctc aaggacaatt ccctggagct 40680
gtaattcaca ccccaccccc agccagttcc atccataggt cagacccctc cagcctcctc 40740
cctgggctct aggcttgccc cctctgattc agcttcctat cccagctggg gacttaggcc 40800
ctactggcca ggctgggtga cctgtcaata ccccagagtc ccagccaaac atgttcccaa 40860
ccctcagacc aacctcattc ttgctgggaa tccctgcccc attcagccac tcagcccccg 40920
gtggctcagc tcttcttcct ttcttatgcc aaactctcaa ggctccctcc ccactggggg 40980
ttcccaggtc tgactcactt tagcattcta ggccagtgct agaggccgca ttaggtgcct 41040
ggtgtttgga tcagaggacc tcagtcaagc caggctccag tctgatcata ggcctacatg 41100
tgggcctctt tgcccttcta ctgaatggac atgcacaccc ctgcccattc tccctccaca 41160
gactgcttct gtgtccagcc cctgttctgt agcaggtgac tcaagctggt tgtaccaagg 41220
gcagcagttg ggaagagtca gctgagcagg gtcctccctc tggcctgccc agggggctct 41280
ggctctgctc tctgccctac tgcttagtta tagccctgcc tcctccactg aattgtgctc 41340
cagctgcatc tctgtatgcc agccagacca gcaaacgctg gagctcaggg tgagggtgct 41400
ggcctccccc actgccctgc cacagggccc tccagcctct agcctctagg agtagcccct 41460
tcagctgggt gagaagcccc atgggataga gcaagtcaga tgcaggaaaa agaaaagaca 41520
gaagaatgcc aagaaataca taaaagtgga agagacccag ggccagacat ggtggtgcat 41580
gtctgtgatc tcaacacttt agaaggagga ggcaggagga tcacttgagc tcaggagctc 41640
aaggtcagcc tggtctacat ggcaaaatca cgcgtctaca cacaccaaaa aaaaaaaaaa 41700
aaaaagtagc tgggcacggg gacgtgcctg tagtcccaac tattctactt aggaggagta 41760
agttgggagg atcacttgat cctgagaggt caaggctgca gtgagccggg atcatgccac 41820
tgccctctag cctgggctgc agattgagac actgtaataa ataaataaat aaataaataa 41880
ataaataaat aaacaaacaa taaaaaagtg gaagagacac aggcaggggt agacagggac 41940
tattttactt aggaaacaac gaggcagttt gttttttcca agcacatggg gtgaagatat 42000
tcttgcccca ctccatttcc ctaccctcgt tctgtgcctg ctgtgccctc tctaaacctg 42060
tattttgaag aatgttttgt attcacaagc aaagatcaag cctctattaa aatagacaac 42120
cagtggggca tggcggctca tgcctgtaat cccaacactt tgggaggcca aggcaagagg 42180
atcacttgag cccaggagtt ctaggccagc ctcggaaaca cagcaagacc tcctctcaat 42240
aataataata ataataataa attggccagg catgtcggtg ggcacctgta gtcccagcta 42300
cttgaggggc tgaggcggga ggatcgctta agcctggaag gtcgaggctg cagtgagcca 42360
tgattgtgcc actgcactcc agcctgagtg acagagttag atgctgtctc caaaaagtaa 42420
aaataataac atagataatt atgtaatagt ttccacaccg gagagtctga ttcttcgtgt 42480
tagtctgcat tcttggcccg actgcaaagc agcggggaca ggcagggatt cgtggccaat 42540
atcccctggg cctgcctgcc cagcccagct ttggtcagct ccactgtgcg aagctcgaag 42600
gggatgccat tcttggcgaa gatgtagact gaacggcagg gctgggacag cagacccatg 42660
gcgggggcaa tggggtgggg cgtcggaggg cagacctaaa cccaggggac gggccctggg 42720
gacaaacccg gggacaggcc cgggaacagg aactgcgggc aggaatggct ggttgggggc 42780
ggcggacagg aacggctggg ggtaggtgag ggatgggcag gaactgctgg gtcaccgagc 42840
ctgcagcggt gcacctcacc aggagccccg agtccggcca cgccctcttc actcatccct 42900
tggctcctag aactttgggt caatcagtgg cgtgtctgct ctctcaccac ccctttctgg 42960
gaatctaggc tgcaggtata tttcccacta aaagggaaga ggagaggctg ggcgcggtgg 43020
ctcacgcctg taatcccagc actttgggag accgaggggg gtggatcacg aggtcaggag 43080
atcgagacca tactggctaa aacggtgaaa cccgtctcta ctaaaaatac aaaaaattag 43140
ccgggcgggg tggcgggcgc ctgtagtccc agctacttgg gaggctgggg caggagaatg 43200
gcgtgaaccc gggaggcgga gcttgcagtg agccgagctc acgccactgc actccagcct 43260
gggcgacaga gccatactcc gtctcaaaaa aaaaaaaaaa aaaaaggaag aggagaaagg 43320
aggtgcagca gcctccttgc ccagtgcctg ggcccagtgc ccaggacgga ggccagagag 43380
gaagggtggc tgactgcctg cggggctgcc agccccacac ctcatgaagg aatgagctgt 43440
ccctccccag ttgctggggt catatttgtt acactctagc taacgcctcc ccgagatggc 43500
tgaggccctg tgaggcctat ccagaggttt ccaacagtgc ctggcattgc acagtaaatt 43560
aatcaccatg acacgagttt tgcaaaagag aaaagattta ttcacagggc accaagcaag 43620
gatgtgggat agtagctctc aaatccacct tcccgaaaat aaggcttagg gataagcctt 43680
gcttccaagc agtagagcac aggccccagc cacgctccag ggtaggggac catacaagga 43740
tgtgaatagc agtgtcggcc ttgtgggcct acaacgcaga agggccatgc acttggttta 43800
aagctttgct gttgccatct taacattctt aacaagttaa aaataagggt ccccacattt 43860
tcatttatag ccagtcctgt ccaaggagtt caaagatctc atgagggtgg acagggcact 43920
gtctgctctg ggcagtgatg ggaccactaa cagttgttct ccagtctccc agcgcctacc 43980
ccagggacac accttcccct ggtctctact attctttttt tttttcgaga cagggtcttg 44040
ctgttgccca ggctggaatg agtgcagtgg tacaaactta gcttactgca gcctcgacct 44100
gggctcaaac aatccttctg cctcaccctc ccatgtagct cagaccacat gcgcatgcca 44160
ccaatctggg ctaatttttt gactttgtag agacggggtc gcactttgtt tcccagggtg 44220
ctcttgaatt ccttggctca agtgatcctc ccacctcagc ctcccaaagt gctgggatta 44280
taggtgtgat ccaccacccc tggcctctct cacattcttg acatttaaag ttaccatcat 44340
tgacaaggaa catgggtaca ctccaattta ctcaagaaaa agctcttttc tccattgaag 44400
acatatgtgg cctagctgct tcatattcta gttgaggtct tcttgttgtt tcctttttct 44460
tcttctctct tgttttttag agacgaggtc ctgctgcgtt gcccaggctg gtctcaaagt 44520
tctagcctca agagaccctc ctgccttgtc ctcccaaagt gttgggatta caggtgtgag 44580
ccactctgtc cagcctcaaa ctgtggtcat tagatcagca gcagtagcag ccttgcctgg 44640
gagcttgtta gaaatatact aacaggctcc cacccccaaa cccagaccta ctgaatcaga 44700
cagagtttat tttcataaga tcctcaggtg atccaaatgc accttaaact ttgaggagct 44760
ttgattcaga acctgggctg gaggccccta ggggccacct tgttcctcac agactgcaag 44820
gtatgttttt ttcttgcctc ttctctctgt cccaatgtct ccttctctct gtgtctgacc 44880
ctgaggaccc tgtgacatcc tcccagggga gtttccactg ccaaaggcct gattctctag 44940
aaactgatgg gctcctctag acaccaatct ctacctgggc tcttgttgaa gaagccaatc 45000
agctcattgg ttggcgatca cgtgatatac aacatggctg ccttccaagc cttatgtgtg 45060
gacaaggaga tagggacaag taggcatatc atgttctgct acctggtcca tcgtctctcc 45120
tcaggccccc aggatacacg tttatgggga gttttcctcc cttcccccaa tatggtcact 45180
cataacttta cacccaacag tcaaacaggg gtggaagcta cagggtttca tgtaaacaat 45240
aggctaggct gtggggctga gtaaaaggca ctggactgtg gtaggtctta ctccaagatg 45300
gccaaatgga agggctttta gctttctttg tttttgtttt atattttgag atataatgtt 45360
caaatttaca cttcagaata aaaaagagcc acaatttatg cttcatattt tttgtagctt 45420
cttccctatg cttttgaaca atgtaatcat attttcatta tcagatataa ttcccatacc 45480
ataatatcca ccctttcaaa gtatacttta aaattcaatt ggtttaaagc caattcagtg 45540
gtttttagta tattaacaaa gttttgtcac catcacagtt atctaattct agatcatttt 45600
catcacccaa aaagaaactc tgtacccaat aaagagtcac tccccattcc cctcttcccc 45660
tagcccctgg ttaccattca tctactttct acctctgtgg ataggaagta gctgcctatt 45720
ctggagattt cattcaaatg taataatgaa atatgtggcc ttttatgact ggcttctttc 45780
atttagcata atggtgtcaa ggttcatcca tgttgtagca tataacggta cttcatccct 45840
tcttatggct gaataatatt caattttatg gactttttag caaaacattc taatttttac 45900
ttctctactg tgcttattgt ttgtctttct ccacaacaat gtgagcacta aaggcagaca 45960
tttttgccta ttttgttcac tactgctttt tctgcaccaa acgcattgcc tggttgtggg 46020
tacttttcta gttttaggaa ggagaagaca agatataggt cctgactctg gttctgcctc 46080
tcattcatat gctacccttc cccttgaaga acttgtttct tcatctacaa aatgggcata 46140
atactactac tcatcagggt ggttgtgagg attaaacaag gtaagagatg taaaattgca 46200
tgttagttat cagatagatt tgtgttccca tctagctcca atgagggaga ggatttgctc 46260
tagtctgtcc ctctgggctc tgggcttcta atgggcagac agcttcctcc atgatgtttg 46320
tagctgttaa ggctggcgta gtccctcaag acaagttttt tttttgtttg tttgtttttt 46380
ttttgagtca gagtatcgct ctgtcaccag gctggagtac agtggtgcga tcttggctca 46440
ctgcaatctc tgcttcccgg attcaagcga ttctcctgcc tcagcctcct gagtagctgg 46500
gattacaggc atacgccacc acacccagct aattttaaga caagcttttg gaattgaact 46560
gctgatggtc ttccatgaac aattcaacag aaacccagta gcgtacactt ccttgtatgc 46620
acttgcaccc tctctttaca tttattacac atttaatgct acctaataat tccctatact 46680
tttactttat ccaatcgttc aacagattcc aaccgaaata catagaagag tgatattcca 46740
acttcatcag accctgagat tctattttta acatttcttc catggattgt tgaatgtttc 46800
caatgattga tccaccttcc cttggccttt tctacattac agatttggtg gcatgtccat 46860
gtctatgttg ccaaacttgc ctcatagacc cagcttgaac acaagatggg tgcttgctga 46920
agagcaggac tcattctgtt gaacctgcat gttttctcag gtcagattgc ttggtggcgc 46980
ttctttaatt tctttcaaca gcacattgta gtttgctgtg ttgaaatgtt gcttttcttt 47040
ggttaaattt attctatttt attctttttg atggtattat aaatggaagt gtggttttaa 47100
ttttgttttt catcctttat tgcaaatgta tactattgat ttttgtgtat taatcttgta 47160
ccctgcaacc ttgctgaact tattttattt tattgagatg gagtctcagt cagtcaccca 47220
ggctggagtg cagtggcttg atcttggcac actgcaacct ccacctcctg ggctctggtg 47280
attctcctgc ctttgcctcc tgagtagctg ggactacagg catgcgccac cacgcccggc 47340
taatttttgt atttttagta gagacggggt ttcaccatat tggacaggct ggtctcaaac 47400
ttctgacctc atgatctgcc cgccttggcc tcccaaagtg ctgggattac aggtgtgagc 47460
caccgtgccc agccttgaac ttgtttatta gctctaatgg tttttaagtg agttaagaat 47520
ttctgtaatg tcatctgtga atagagatgg ttttacttct ttctttctga tctagattcc 47580
accttgttcc tttttcttgc ctaattgccc taataagtag aagtagtgtg aatgaacatt 47640
cttgtcctgt tcctgatctt aggggggaaa cgcttgatct ttcacaatgg atgaagtatg 47700
gtgttagtta taggtttttc atagatgctt tttgtcaagt tgaggaagtt cctttctcgt 47760
cttcatctgc tgagtaattt tatcgtaaaa ggatgttagg ttttgtcaaa tgccttctgt 47820
gcattaggat gatcatgtga ctttctatta acatggtatg ctacactgat tgatttttgt 47880
atgttgaact gcatttgtac tcctgggata aatcctacct ggtcaaggtg tatgatcctt 47940
ttaatatgct gctggatttg atttgctaat attttgctga ggatttttac atctatattt 48000
ataaggatat tgctttgtaa ctttattttc ttgtaatgtc tttttctgtc tttggtatca 48060
gagtaattct ggcctcatag tatgagttga aaaatatttc ttcctgtttt atttttttgg 48120
aagagtttgt gaaggctttg tgttagttct tcaagctttt ggttgaattc accagggaag 48180
ccatctggtc ctgggctttt ctttgtggaa tttttaaaaa taattaatat tttaatctct 48240
ttatttgtta caggcctatt aaaattttat ctttcttctt aagtcagttt tggtagattg 48300
tgtgtttcta aaattttccc atttcatcta ggttgtctaa attgtccaca tatagttatt 48360
catagtattt ctaaactttt gaatttctct atgaccaatg tgatgtctcc attttctttc 48420
tttctggttt ccatttcatt ttttcatttt tgtttttgtt tgttttttga gataaggttc 48480
ttctatgtgc ccaggctgga gtgcagtggt gcaatcatag ctcggtgtaa ccttgaactc 48540
ttggactcga gtgatcctcc cacctcggcc acccagttag ctaagactac aggcttgcac 48600
caccacacca agctaatttt tttaaaaaat atatttttta gaaaaaagtc tcattgtgtt 48660
acccaggctg ttatcaatct cctggcctca agtgatcctt ctgcctctgc tttccaaagt 48720
gctgggattg caggtgtgag ccattgcacc cagcctccac tttctttctt ggtgaataat 48780
ttgaccctta tttttttcac ttggctattc taagtaaagg tttgccaatt ttgttgatct 48840
ttgcaaagat ccaatttttg gttttattga ttttattgct tttctatttt ctatttcatt 48900
tatctccatt ctaatcttta ttatttcctg ttttctagtg gtgttgggtt gagtttgctc 48960
ttatttttct agttccttaa ggtgtaaagt taggttattg attcaagatc attcttcttt 49020
aacttatgtg tttacagcta taaattttgc tcttagaact gcttctgctg catcctataa 49080
atctgggcat gttgtgtttt catttttatt tgttcaggat actttttgat gtcccttgtg 49140
atttcttctt taacccattt gttgtttaag agtgtgctgt ttaatttcta tgtgcttgtg 49200
agttttctag ttttccttct gttcttgatt tttaacttta ttccactgtg tccagagaac 49260
atactttgtg tgatttcaat catcttaaat ttgagactta ggaccaaata tatgctctat 49320
actggagaat attccatgtg ctctttagaa gaatgcttat tctgctcctg tcgggtggaa 49380
cgttctgttc atgtctagtg tatgcgattg gttaatcgtg cttttcaaat tccctatttc 49440
cttgatggtc ttctgtagtt tttctattac tgaaagtggg gtattgaagt caccaactat 49500
tattactcac aatgtaacca tatttaaccc tttactttta aaattctttt tggaaactgg 49560
aaggatctgt aaccaccacc cactcccaca tcagacccta tgcatatgcc aactgtctgc 49620
tgctcagcag ctgtgatggt tgttccagtt ctttattagg caaagaacag ttgttttttt 49680
gtttttttgt tttttttttt ttaacatttc ctttaaggaa ggtggctcag attgctaagc 49740
cagccaggcc ctgcgggaca ggctgcgcct agggtcacct gctcttcttc agcaactcag 49800
aaatattctc cttgaccatt gaatccaatg ttgaaaagtc ccagtcggcc aactgcatta 49860
gtcgatcatg ggcctcccta aagaggccag agccaatatt cagctccacc tgcatacgcc 49920
actcagctag cttggagctg ttgaggaaga cattatagtt ggctgccatg ggctgtagat 49980
agacgaagac aaagacgtgg tcagcctggg gaccagcccc acctgggtcc tctcccacag 50040
cctcaggccc acgtccctaa catctaccta tgcaaagaat taggctgctg acccccaagg 50100
cctgagcaag gggtcacaga ctatgtaata gatgtaggtg gggatggagg atccagaatc 50160
cctcagagtc ctcagtcact ggtcttcttg ctccaagcct tctgaaccat gctgagaggg 50220
ctcctggccc aggcacttcc cacttctcca gcctgcgacc tcgcatgaat cctgccttct 50280
tccagggaaa ccccttatcc caggtgtgta tatgtggatg agggagagaa ctcaggtttt 50340
ttcctccatg tttagcctcc cactgtgatc aagctcaggg gctaggatgg gagacctggc 50400
gggcagtcta ccctgcagtt tcgctggctt actaagagtt tggtttgcac ccaagatctt 50460
tgggaagccc aagaatgggt gtgtgtgggt gaaatgtaag gggtggggac gaagcatatg 50520
gctgaaccct tggggcaggc cagaatgatt tttcctggtg ctggtctgcc ctgcaaacag 50580
accaaggaga ctaattttca tatcagccag agatctctgg gataaggaaa agaatactgc 50640
attttctggt caatccacca ggaccccagg tccctcctct cgggcatatc tctggctgat 50700
atgcaaatta gtctctttgg tcagtgtgca gtgccctagc tggtgtgcag gatggccagt 50760
tgagaccctg gccagtgtct tgacaagcag aactggtcac cctcccctgc atgtagaggc 50820
cacataaatg ccccacactc aggtgtgcct ccaaatgcac agtggatgcc cctcagaccc 50880
agccacgaga gctgtcctcc agagctgtct gtctggagct ctgggaaaca ggcagggcca 50940
gaaggacacc caggaagcca gtgaacattt cctggagagt ccagcaagag gaggaggtat 51000
ctgggatgct ggtggattga gcaggaaatg cagtgttctt ctctatccca ggctcaccct 51060
ccgggtcctc ccacaccgaa gaatctttgt caagtgtgga gaactgtgat ccttcctgat 51120
tcataacatt ctgtgcttcc tgttgccccg attgagtcca ggcccccagg cctggttccc 51180
gcagccccca tggcagctct gcctgccttt cccgcctcac cagcctatcc tcaagtgatg 51240
gccccattgg tcacagagga gtcctacctc tgcccagggt ctaaccctcc tccaatccac 51300
tccacacctg catcatctcc accacggcca ccaggtcagc cagtgagatt tggttcccgg 51360
tgatgaacat cttatcctgc agaaaatact cctcaaagag ctgcaggctg ttcttcacct 51420
cttccactgc atgctccatc ttctcagctg aaacttcctc ccctgttatc tttgggatca 51480
gcaactggcc agggttggga agaggaggga agaggaggct gcactccagg gccacctgcc 51540
ctgccaggtc tctgtactct tgtctgctgg atagatattg aacacttccc aggatataaa 51600
gcagtttcac ctcttttagc agttctgatt ggtggaagtt gctgggaacc atgtgttcac 51660
aaggatttgg ggagctcagc aggcataagt cctgtgattg attagtgatg tctgtcacag 51720
gcatagaatt caaagtagag acacatgtac tggttatttg tcatcttcta attttctata 51780
ggccatactc ttttttgttt ttgttttttg agatggagtc tcactctgtc gcccaggctg 51840
gagtgcagtg gcacaatctt ggctcactgc caactccaac tcctgggttc aagcaattgt 51900
cctgcctcag cgtcctgagt agctgggatt acaggtgccc atcaccacac ccagctaata 51960
attttgtatt tttagtagag atggtgtttc acaatgttgg caaggtaggt cttgaactcc 52020
tgacctcaag tgatctgccc gcctcggcct cccaaagtac tgggattaca gttgtgagcc 52080
accgtacccg gccttctgtg ggccttttga cgctgagatt ctctagttca gaatgaaatg 52140
cctcgaatgc tgtcctgggt gagtcacatc agcccctccc gtatgccctt cccctcgccc 52200
taataagact ctttcatgcc cattgtttca gtccacactc ctgacggctc tgtaaggcgg 52260
gcaggagaag gagctgagga aatgaggccc agagagggag ggagacttgc ttgaggtcgc 52320
cggcagtcag cagggccaga actggcctcc agccttcctg acttccctgt gcccgtgtcc 52380
ccaagcccca gaagtggccc tgctcacctt gagccagact atcttcttca tgggcagctg 52440
aaaggccgtg tgttgccaag ccacgaactc atccacacgg gcacgtgcgt gcaggtctgg 52500
cgggcaccag tgcgatggtg cgctgtactt gcggcacagg tagtaaagga tggccgcgct 52560
gcagaagggg ccggtcaggg gcactgccct tgccttcctg agtgccacta cgtcaaccac 52620
cccggtgtgg cctgggccca actgctgggg cttccagagc aaagaggagc ccaaacggcc 52680
ccgagaaaga ccttcaccag agctgtctgt ctgacagtca gtaagggctg ggaaggagcc 52740
ctgcggggtg agtaggagtt gggggctggt ggtataacaa agagtaggcc agcaggggga 52800
acaacacgtg ttgaattggg atgctgaggt gggaggatca cttgatccca ggaatttggg 52860
gctactgtga gccaagatca caccactgca ctccagcttg ggtgaaagat caagatcctt 52920
tttcaaaaac aaaaacgggg gggcacgatg gctcacacct gtaatcccgg cactttggga 52980
ggccaatggg ggcagatccc ttgaggccag gagttggaga ccagcctggc caacatggtg 53040
aaaccctgtc tctactaaaa tgaaaataca aaaattagct agttgtggtg gcacacacct 53100
gtaatcccag ctacttggga agctgaggca cgggagtcac ttgaacctgg gaggcagagg 53160
ttgtagtgag ccaagattgt gccactgtac tccagcctgg gccacagagc aagactctgt 53220
ctcaaaaaac caacaaagaa aaacacatgc tgaaatacga gggtaaaggg agcaaggtaa 53280
atctgaagaa aagagagtag ggggttgcaa ctggaagaag ggtgggggtg attggggagt 53340
gatgaggcag ccagagacac tgtggagtcc acgtagggta gcccctggag gtgcagggag 53400
gttatggact taatgcttaa gattaggcat tatataagcc agggcatgaa aggatccatc 53460
tctctggtgc tggatggagg gtgagcccga gggggcagaa tggacaatga ggtggcgggc 53520
cagcaactat cgggaaggtt gtggtgtctg gggatggtgg aggccatggg gacagaggga 53580
aggggacgga ggggagacat gcttcggagg ggatgtccta ggccttgctg attgatggct 53640
ggtgtgggaa cctccgcagc acaagggctc ctttatcatc accaacagca accatgccaa 53700
ggtaaaaagg tcagggcatg gagagagcta tcggttaaaa agtggcagga gagacagcaa 53760
ctggctgcaa gactcagaac ttctttggat cctgatccaa acaaactgcc aagaaaatat 53820
ttaggagata atcagagagt tttgaacagg agccacgtat tagatgaaat ccaggaatta 53880
ctgttaattt tatgaggtat cttaatggta tcgtagtgat gctacgctct atcctagccc 53940
aggctggagt gcagtggcac aatcagagtt cactgcagtc gtgaactcct ggcctcaagc 54000
gatcctcccg tgtcagcctc tcaaagtgct gggattacag gcatgagcca ccgcgcccag 54060
cctgttgctt tttttttttt tttttttttt tttttaaaga acgcttatct ctaagaggta 54120
tgttcctgaa cgtttattga tttatttact tacttatttt tatttttgag atggggtctc 54180
actctgttgc ccaggctgaa gctcaatctt tgctcactgc aacctctgcc tcccgggctc 54240
cagcaatccc tccacctcag cctcctgagt agctgggact acaggtgcgg accaccatgc 54300
tggctaactt ttgtattttt tgtagagatg ggggttttgc catgttgtct aggctaggct 54360
ggtcttgaac acgtgagctc aggccatccc ctcacttcag cctctcaaag tgctagaatt 54420
acaggcatga gctggcccct aaacatttat gaatggaatg atggggtgtc tgggaggcag 54480
gggaatagaa atgatgtaaa ctggacccca agttggcaag agtcagagct gggcgatgga 54540
tttgtggggt tcctcgtgtc cctcattagt tagtattcac tctcctttag tgcacgtgtg 54600
agattttcca tggtcaaaca gacaaatgct cgcactgaac ctcccaggag gagcagagac 54660
agatggtgca agggccccag ggaagactta cctttcactt aagataaatt tcccatcttt 54720
gaggctggga agcttcctga gggggttgat gtcaatgtat tctttgctgt ggtggtgacc 54780
tgggaggagc agggaaggtc tgaggctgtg ggactccagg ggagagagaa ctgagactcc 54840
cagagacaca aacgcctccc tctctatttt ctcaagaaga gaggactgag gcccggaggg 54900
acatggcggc tcaccccagg tcacagggca aggcagttgc agaaccggac tgggatcaga 54960
actgctggct cccagcctgc tccaccctag gtttggtgac tcccgtgcct cctacctgtg 55020
tcccaggacc aggacgaccc ttttacccag aagccggagg cctccagtgc ccacccccaa 55080
agctggatct gaaaacacag cctttgaatc acctgaagcc ctgagggcct gggtcccatc 55140
cgcaatccca tcgctctcac tctgtctcca ctttaaggaa gccaggccca gcacacagct 55200
ggacatccaa agggaagctt ctcggacaca atcagggtca tcttaacagg gaacctgagg 55260
tgggggcagg aactgaaact cttcctggac cagccgcctc cagttggaaa catttctggg 55320
ggctccactc gcagcccgtt catttccaca gcttccctgt ctcttcctct gtgttctaga 55380
ggcttctgct tttgcaggct gagttttgga gtccctctgt gctcgggatg gagttggagc 55440
ccacccctct gaccctcact cgggagcagt ggagccctga gcctttctga acactgggga 55500
ggatgggtgt agacggactg tgcacttctg ccccctttgc caacctggtg ggcaggtgct 55560
gagttcacaa ggtcctagaa tcccacaacg aagccagggt gcctggtggg agcccaggga 55620
gtcccagctg ctgttcattc ccccttctcc tcaaaaagcc tgttcatctg tggcgtgggg 55680
actatcatta gtggggagtg gctaaggtag gctaggcagg gacgcagcct acaagccccg 55740
tggcatcttt tccttccctg tggacctctg gggtgattcc cttgtctctg tccctgcttc 55800
tcagaaatgc ccctatcaat ctgagcgtgg tggctcacgc ctgtaatccc agcactttgg 55860
aggctgaggc ggggcagatc acttttggtc aggggttcga gaccagcctg gccaacatgg 55920
tgaaactcca tctcgactaa aaatacaaaa aatgagccgg gcatggtggc gtttgcctat 55980
aaacccagca actcgggagg ctgagctggg agaatcactt gaacccagtg gagattgcag 56040
tgagccaaga tagcaccact gcactccagc ctgggtgaca aagtgaggct ctaccaaaaa 56100
gaaaaagaaa aaaaaagctc caatctgtgt gtcctgcctc cccccaggac caggcctgcc 56160
aggcagcagt gggagctgac ctttcagcag atccacaaac tgaaagttga actggatgtc 56220
atgcttcttc gagaagatgt agacggcacg gcagggtgct gacagcaggt ccatgtagag 56280
ctccagggcc atgttgagac acatgccagg ccccacagct gcagctggcc agccacagac 56340
ctgggcctat gtctggccag agtccctggc cctgtgccct ctccgatctg ggcccaggac 56400
cctgcattct ggaacctctt gtttcccttt gtgttgtcat aaggccagga agcctgcaat 56460
tctcacagca tcaaggattc taaggaggcc caggagtagg ctggcagagg cccgtggcaa 56520
aggtgtggca gccgtgaccc tactctcccc ctctcacgtg tgtctgtgtc ccgtggcgcc 56580
acctcacaga caccctgtct gagaagggat tatgcctggg aattcccagg gccggacttt 56640
gattgcagaa cctgacgaaa ggggctttgc agggtccaga atgaggagga ggcaatgaga 56700
attatccctg gaggattcta gaagtagagg ctgggagcat ccacaggtga atggagcctg 56760
aactatgact agaaaggaat tgggagaaag agacacagga acctgggagc ttcgggagca 56820
gtggggacac caccaccagg aagtcagggg gcactcggca atgaaagtgg cagatgcaat 56880
aaaactttta acatattgtt gtataatgta caataatgta ataaaaatac accccctata 56940
cattattgtt ccaactctag ctctatctat tgccctcctc atatgaatcc ctctccccat 57000
accccattct ctagttaatc ttaactaggc ctcctgttta tattagccac atcaagccta 57060
gctgtctact ctatcctgtg atcaggatga gcatctaatt tgaaatatgc actcatcggt 57120
gcactatgta cagtggccca aacagtctca tatgaagtca ctctagctat tatcctccta 57180
tcagtcctat taataagtgg ttcatttaat ttgtatatac tcattggaac agaagaattt 57240
ctctggctgc tcctaccatc atgacctctg gccataatat gacttatttc cacactagca 57300
gaaaccagcc gagcctcttt agacctaaca gagggggaat cagaattaat ctcgggcttt 57360
aacgtcgaat atgctgcagg cccatttgcc ctgttcttta tagcagacta tataaatatc 57420
attataataa atgccctgaa ggccgcaaga ggttcgtggg tgcagtgcac aggagaccat 57480
atctggggac agcagctgca gcagacccca agctgggcca tccctgccac ttgccaggtg 57540
gtgcttggcg aaggcaagca gctcccaccc gcccggggaa tacagcgcga cccccggcgg 57600
catgctcttc agcaccaccc caggaggtac caggatcatc taccactgga aattcctgat 57660
ggagtgttga aacttacccg tgaccaaaac actcccaagg gatctgccca ccattcccgg 57720
ggtcaccagc cctgccagtg atgatccccc caacggaagc cagccagagc cacctgagca 57780
atagtccaga agataagcag ggcgggcagt gaagaagcac agttttgaga tggacattta 57840
aagcaccagc catcgcatgg agcactgcca agggacccct caggcccttc ctggaggagt 57900
cccaccagcc aggccttatg aaagtgatca tactaggcag gcattggtgt ggggccagtc 57960
accccagccc tctctccctc actcgcggca cctgccccct cctctttgtg aacaccagca 58020
ggtacctcct tgtgcctccg ctgatgcagg agcagctgcc ccaaggggag taacccctgc 58080
cagcacaccc tgcagctgag ggccaggaag tggacaagaa tgaacccttt cttccaaatg 58140
atcagctctt ccagcccctg ctgggggccc cacgacccct tccttaggtt gacgtgcctg 58200
ggaaagctca cctccccctc ttccctaaga gaggaaataa aagccacctt cgccctaggg 58260
caaaaaataa ataaataata aatcccgtaa tcactattgt cttcctagga gcactgcata 58320
acatatatat accagaactc tataccacaa atttcgtcac ccaaactctc attttaacaa 58380
gtcttttttt atgaattcga acatcatatt cctgattcca ttatgatcag ctcatatatc 58440
tccttgaaaa aattttctac cactcacact agtactttca tatgacatat cttcatgcca 58500
gtcctaacct ccagcatccc accccaaaca taagaaatat gtctcacaaa agagttactt 58560
tgatagaata aattacagag gtttaaaccc ttttgtttct agaattacag gaattgaacc 58620
tacccatgag aatccaaaat tctccgtgct acataataca ccacatccta tcacacccca 58680
cagtaaggac agctaagtaa gctattgggc ccataccctg aaaatgttgg ttataccctt 58740
cctatactaa ttaatcctcc agtgtatgcc atgcagagga gcccagaaac ttcctggcct 58800
gggttggggc tgcagtggcc agactgtgca cctggtggcc caggaaggta actagagccc 58860
cacgtagagg actgagtgcc actcactcta tgctttgtga tttaataggt ctaggctcag 58920
aaatgggact gatcccactt ccagtgacag agtaagcctg gagacaagcg aagagcatgc 58980
agtgtgttta ttgcagacag cagggtgcag tgcagtgggc tgtacccact cagtggagcc 59040
actcctgcag caactgcacc tgcttctggg ccagctgggg gtcctgccga gcctcactgt 59100
ggttcagagg ctggagtatg agcttgtggg cctccaggaa gagctcagta ccaagggcag 59160
ccttcatatg ggcctgtcac actgccagcc agggccagtc ttggaagagg tcgcagccaa 59220
cggcagtggg ctgggaagac agggcagctg gggttggggc tcagccccat gcccagagtc 59280
cccccgaggg caggtgggta ggaaagcttc acctgcatca cctccgtcag caccaagtct 59340
tccagggaga tctgctccat tgccaggaag ggcctggcca ccaggacccc cccatccagg 59400
tgctgcaggg ctggcgtcag cctccccaac agccgctcca gctgtgcagc atctgcaggc 59460
tgtctggaga agtggggcag gagagactgg gctgtgagga gaggagcaac ttaacccaga 59520
ctcttggaag cctcttgggc ctcctctcca tcccttcccg gctgccattc attagtgatg 59580
tcctctgggg ctctgtgttc agaaggtttc taaggattca ttcaggctta ggggaaggag 59640
tactgtgata gattagttat gtctgctgag gacagtggaa gtgaagctaa tatgtaatat 59700
gtaccaactg gtaccttcca tctctggggg tccaggcccc tgccccagcc tgcatgacag 59760
ggcctaggaa gctcaccttg cacaggtaga cattggtggc aggcagctgg atggtgacat 59820
gcttccacgc caagtactca tccacgcagg tgcgggcttg cagctcaggt gggtaccagt 59880
gtccccgtat ctggtacttt cgactcaggt ataaaacgat gaccatgctg tgggcagggg 59940
gtgtcagaga tcttttctcc agcctgtcag ggccaggtgg cttcattgct catactcagg 60000
gtgatttgac caggttttct ccccaccttc accttcaggg gaggacctcc cctatactgc 60060
aactccctcc aggcagtatc tccccacgac caaaccactc tgtttctcca tttccaactc 60120
tgatcctccc tgggcccatg tttccacctc ccctcccagt taccatacca tcctgccctc 60180
tgtgcccagg ccccactccc tggcatcagg gtccccactc agagtgcctg ccgtgtgggc 60240
cctgcccaaa aggtctccaa gttcccttcc tagtgcccca gaacactagg tgcccatttt 60300
ctatcctcag gtaagccctg ggaggtggat acattattcc tcccactccg ttttacagag 60360
gagaaacctg gcacacaaag ttctgtgatt tccccaaggg catggattga ggaagtggtg 60420
agctgggatt gaatgtggag tctcccctgg gtctgcccag ctcctaggac tctgaaagga 60480
tgagctgggg acatttgggg aggagccagc ctcaagcctg ggccttgccc gctggcttca 60540
ggttgtccag gtttatctgg gtgacctacg tcctttcccc atctgggcag ctccagctgg 60600
cttagactct agccaggttc cagcctccag ctttagccca aaggtgcccc tgggtccagc 60660
ctctgcatcg gagctacaca ggctgaaatg aggaaggggc cacagtgatt tggaaagatc 60720
tccaacccaa ggaaagatct cccctctcgc ctctgcctcc ccctacaact ctgagcccca 60780
gaacccaggc agatcctggc tctgaccctg ggtgggcacc aggtcaccat tacctctctg 60840
ctagtaggaa gtcgccatct ctgagggcag gcaccttccc cagggggttc accttcagga 60900
actcaggctt caggtgctcc cctggtgggc agaaaggaga gaaggctcag tgcagggcct 60960
ggtccttggc acccactcca tggggcccag cccagccacc tggctcaccc caagccaact 61020
ccactgtctg cagctgaaaa gggatgtcat tctgcctggc aaaagtggat agcatggcag 61080
ggggctgagt gcatgtccag gaacagcttg ggccccattg ctgctcctcc cacctgccct 61140
ccccagaggt ctgtacctcc aggagttgct cagcccttct cggccagtgc cttcccccaa 61200
aatggggcca gaactctgta ctcctcctga agcctattcc ctccatgact ggctgggagg 61260
cctgccaagc ccctctcccc ttttctccac ctctgtagag tggaggcagg ggtggccagt 61320
gtccctttgg gggtttcaga tagaaactca atgacaattg gcttagcaaa aacaaacaaa 61380
caaaaaaaaa acaacaaaaa aaaaacaaag ggagtggggt gggtggggag atttctcaga 61440
aaacttctcc agtcatagga ggggaggggt gtagaagcca ctaagagaac tggaatcaga 61500
acctgtttct gtctccccac ttttcctccc ctctcctcct ctcccctcct tcccctcctg 61560
tcccctcccc tcctctcccc tcccctccct tccctttcct tccttccctt cctcctttcc 61620
tcccttcttt ctttgagaca ggatctctgt ctccctacac ttttttcttt taattctctg 61680
tctctttctt gacatagaat cttgctctgt cacccagcct ggagttcagt ggcacgatca 61740
tggcttactg caatctccaa gtcctgtgct ccagcagtca ttccactaca gcctctggag 61800
tacctaggac tacaggtgca catgaccata ccccactagt taaaaaaaac aaaaacaaaa 61860
acaaaaaaac ccttgggcct ggcagggcgg ctcgtgccta caatcccagc cctttgggag 61920
gccaagatgg gctgatcaca tgagcccagg acatcaagac gagcctgggc aacgtgccga 61980
aacccagtct ctacaaaaaa tacaaaaatt agctgggcat ggtggcgcat gcctgtagtc 62040
ccagctactc aagagactga ggtggcagga tcacttgagc ccaggaggca gaggttgcag 62100
tgagccaaga tcacgccact gcgctccagc ctaggcaaca gagccagact ctgtctcaaa 62160
aaaaaaaaaa agagagagag acagataggg tctcactatg ttgtccaagc tgatcacaaa 62220
ctcctggcct aaagtgatcc ttgggtctca gcctcccgag ctgattagct gggattgcag 62280
gtgtgggcta ccacgccctg ttatcactcc ctttctctga gtcagctttt ctctctcaga 62340
ctagcctact ccatgaactc aggaaatgtc actacaggtg gccccagctc ccatttacct 62400
ccccataggg agctcctctc tccagttcca atttcaaaac tcccaaggaa gcattctggt 62460
tcactcgctt gggccactgg ccagagggat gggatactct gaaagattca gctagagtcc 62520
cgggcccagc cctggaccat cactgtgcac cctggttaga tgccagggct gggattcagg 62580
gagaagaaag gaggttcccg gacagtcatt cctgcctcct gcggctgcgg gctccctgca 62640
cagtcattcc tgcctcctgc ggctgcgggc tccctgcccc catcctgtgc acgaagtggg 62700
agctcccgct gtctggcagc agctgctctg caggggacag tctggacggc agaaagttca 62760
tccttaaccc cagccttcca gtcaaggttc ccacaagtct gggacacctg caagtgtcat 62820
atcccgctgg gtgaaagtct gagatccctt ttagaggatc ccattcgctc cctcccttct 62880
gccaccttcc agctccgaga aacagagctc tgaacgaacc ctcagatgtc catgcgctgg 62940
ggcctttcca ggacggcggg gcgcagtcct ttctgggttg gggtgacacc tggaaacatg 63000
taaggtccct ggcacaggga cccctcccag tttcaagcga ttctcctgcc tcagtctccc 63060
gagtagctgg gattacaggc gcccaccact gcacccagct aattttttgt gtttttagta 63120
gagatggggt ttcaccgtgt tagccaggat ggtcgcgatc tcctgacctc gtgactagcc 63180
cgcctcagcc tcccaaagtg ctgggattac aggcgtgagc caccatgcag ggccaatttt 63240
tgtattttca gtagagacag ggttttacca tattggacag gttggtctcg aactcctgac 63300
ctcaggtgat ctgcctgcat tggcctccca aagtgctggg attacaggcg tgagccacca 63360
agcctggtgg taatttataa ggggtggaca tttgttggct catggtcctg aaggctggaa 63420
agtccaagat cgaggggcca gcatctggcc agagctttct tgctgtatca tcccctggtg 63480
aaaggataaa gagagggtaa gagagagcaa gaggctgaac tcacagcctc aaagtccttt 63540
tataatccac atcaatccat ctgtaacggt ggaggcctca tgagataaac acctctcatt 63600
aggccccacc tcccaacact gttgcattgg ggattacatt tccaacacat gctttgtggg 63660
ggacccattc aaaccacagc acagggacac cttctctttc cttcctgtga tatgctgaat 63720
aatgacccct aaggatatac aagtcctgat ctctggaacc tgtgaatatt accttctgtg 63780
gcaaaaggga ctttgcagaa ttgattaaga atgttgagat ggaggcattg tcttggatta 63840
tctgagtgag ccctaaatgc agtcacaagt atctgtgtaa gaggagacag acggagattt 63900
gaaacagaag acaggagaag atggaagcag agagatttga agatgctaca ttgtgggctt 63960
tgaagatgga agaaggggct acatgaccca agacaggcag ctccaaaagc tggaaaaatt 64020
gaggaaacca attcccctct cagagcctct ggagggtgtg caaccctatt ggcacctggg 64080
ttttggccca gagaaataga ttataaagtt ctggtctcca taactgtaag agaatagatt 64140
tatgttgttt taagccacca aggtggtggt aatttgttat accatccaga ggagaggaac 64200
acagattttg gtgccaggaa gcagggtgct gctataaaaa agaaaatcta ggctgggcac 64260
agtggctcat gtctgtaatt tcaacatttt cggatgccaa ggtgggtgga ttacttgagc 64320
cctggagttt gagactagcc tgggcaacat ggcaaaaccc cgtctctact aaaaatatat 64380
aaattagctg gggatgggcc gggagcagtg gctcacgcct ataatcccag cactttgggt 64440
ggcaaaggca ggcggatcac gaggtcagaa ggttgagacc atcctggcga acatggtgaa 64500
acccagtctc tactaaaaat acaaaaaatt aaccaggcgt ggtggcgggt gcctgttgtc 64560
ccagctactc aggaggctga ggtagcagaa tggcatgaac ccaggaggcg gagcttgcag 64620
agagccaaga tcgtgccact gcactccagc ctggggaaca aagcgcgact ccgtctcaaa 64680
aaaaaaaaaa attagttggg gatggtagtg catgcttgta gtcccagcta ctggggagac 64740
cgaggtggga ggattgcttg aggctgggaa gtctaggctg cagtgacctg tgactgtgac 64800
actgcactcc agcctgggca acagagtcag accctgtctc aaacaaacaa acaaacaaaa 64860
atctaaaaat gtggaagtag ctttaaattt gggtgatgtg cagaggccag aaaaattatg 64920
aggcccatga tagagaaaac ctagattgcc ttgcacagat tgttggtaga aatatggatg 64980
ttaaagacta tagggagggc tcacgagtat caactaaaca tggtcagagc aagtctacat 65040
tgccttacag catacctaag tcatcatagg cagctgtagg tagatatata gatattggct 65100
gggtgcagtg gctcacgcct gtaatgccag cactttggga ggctgaggtg ggtggatcac 65160
ctgaggtcag gagttcaaac cagcctggcc aacatagtga aaccccgtct ctactaaaaa 65220
tacaaaaatt agctaggcgt ggtggcatcc cagctactag ggaggcggaa gcaggagaat 65280
tgctcgaacc tgggagacgg aggttgcagt gagccaagat tgcgccacta tactccatcc 65340
tgggcgacca agcgagaatc tgcctcaaaa aaagaaaaaa tagatattaa aaatcctgct 65400
gctgaggact ctgaagaaat gaggaacgtg tttctggaaa ctggaggaag gaggatcatt 65460
tcccagaaaa ggagcccgta gggaggtaga aagcttagtt gaattgtgtc cgatagttat 65520
atggtgggtg aacttaaagc aacgaagttg gatatttagc tgaggagact tccaagcaaa 65580
gcgttgaagg tgaagcctca tttcttcttg ctgtttatgg tgaaaagcaa gaggaaagtg 65640
ataaaatgag gaaaaagctg ttaagcaaaa agaaaccaag gtttgatgat ttgggaaatt 65700
atcagcctgt gcagattgca aaagatgcta aaattagaag attcgctgtt tggaaaacac 65760
aactctatcc ctgtcctggg gatcactcaa gacttgtatc catagacaac cagatttcta 65820
tgggcttgaa ctctgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt 65880
gtgtgtgtgt agcaaggttg ggggagcaca gtggaaagac cttgaattta tttgtttatt 65940
ttttagacgg agtcttgctc tgtcgcccag gctggagtgc aatggcacga tctcagctca 66000
ctgcaacctc cacctccagg gtggttcgag cgattctcct gcctcagcct cccgagtagc 66060
tgggactaca ggcgtatacc actatgccca gctaattttt gtattgttag tagagatggg 66120
gtttcaccat gttggtcagg ctggcctcaa attcctgacc tcaggtgatc aggctggtct 66180
caaactcctg acatcaggag atccaccagc ctcggccttc caaggtgctg ggattacagg 66240
catgagccac catgcccagc cttattattt atttatttat ttttgagaca aagtctccct 66300
ctgtcaccca gactggagtg cagtggcgca atctctgctc actgcaacct ctgtctccca 66360
ggttcaagtg attctcccag ctcaacctct caagtagctg ggactacagg tgcgcaccac 66420
catgacctgc taatttttgt atttttattt atttatgtat tattttattt tatttttcca 66480
taagttattg gggtatgggt ggtatttggt tacatgagta agttcttttg tagcaatttg 66540
tgagattttg gtgcacctgt caccccagca gtatacactg caccatattt gtagtctttt 66600
atccctcacc ctcctcccac tcttcccctc aagtccccaa agtccattct tatgcctttg 66660
cgtcctcata gcttagctcc cacatatcag tgaaaacata agatgtttgg ttttccattc 66720
ctcagttact tcacttagaa taatagtctc caaactcatc caggtcactg caaatgctgt 66780
taattaattc cttttcatga ctgagtagta ttccacatat acatattcca tatatattca 66840
ttccatatat atattccata tatgctcatt ccatatacat attccatata tgctcattcc 66900
atatacatat tccatatata ctcattccat atacgtattc catacgtatg tactccatat 66960
acgtattcca tacgtatgta ctccatatac gtattccatg tatgtattcc atacgtatgt 67020
attccatgta tgtattccat gtatattttc catatgtata tattccatgt atatattcca 67080
tatgtatata ttccatatat attccttatg tatatattct gtatgtatat actccatgta 67140
tatattccgt atatctatat tccatatatg tattccgtat gtatatattc catatgtact 67200
ccatatgtat tccatatgtt ccatatatat tccatatatt ccatataaat tccatatatt 67260
ccatatatat cccatataca ttccatatat tccacatata ttccatatac attccatata 67320
ttccacatat attccgtata ttccatatag attccataca tttattccat atatattcca 67380
tatagattcc atatatattc agatatattc catatttata ttccatatat ggaatatata 67440
tgtattccat atatatgtat tccgtatatg tatgtattcc acatatatat tccatatatg 67500
tatgtattcc acatatatat tccatatacg tatattccag atatatactc catatgtata 67560
tcccagatat atatattcca gatgtatata ttccacatat atattccaga tgtatatatt 67620
ccatgtgtgt atattccata tatgtatatt ccatgtgtgt atattccatg tgtattccat 67680
atatatattc catgtgtgta ttccatatat atagtccatg tgtgtattcc ctatatatat 67740
tccctatata tatattccct atatatattc cccatgtaca ttccatatat atattcccca 67800
tgtacattcc atatatattc cccatatata ttccatatat attccccata tatattccat 67860
atatatattc cccatatata ttccatatat atattcccca tatatattcc atatatatat 67920
tccccatata tattccatat atatattccc tatatatata ttccctatat atattcccta 67980
tatattccat atatatatat atatatatat atatatatac accctatata tatattccct 68040
atatattcca tatatatatg gagtctcgct ctgttgccca ggctggagtg cagtggcgcg 68100
atctctgctc attgcaagct ccacctccca ggttcacgcc attctcctgc ctcagcctcc 68160
tgagtagctg ggactacagg tgccagccac catgcccagc taattttgtt ttcgtatttg 68220
tagtagagac ggggtttcac catgttagat aggatggtct cgatctcccg acctcgtgat 68280
ccgcccacct cggcctccca aagtgctagg attacaggct tgagcctccg cgcccggcct 68340
ctgaattctt tttcaggtaa atcagggatt tcttcttggt ttggatccgt tgctggtgaa 68400
ctagtgtgat atttgggggt gttgacgacc cttgttttgt catgttacca cagttggttt 68460
tctggttcct tctcattggg gtaggctctg tcagagggaa ggtatagggc tgaaggttgt 68520
tgttaagatt ttttttgtcc cagggctatt cccttgatgt agtctctccc ccttttcctg 68580
tggatgtggc ttcctgtgag ccaaactgca gtgattgttg tttctcttct gggtctagct 68640
gcccagtgag tctagtcagc tccaggctgg tactgggggt tgtctgcaca gagtcctgtg 68700
atgtgaaccg tctatgggtc tctcagccat ggataccagc acctattctg gtggaggcgg 68760
cggacggtgc agtgcactcc gtgagctttg gtggtttaat gctctatttt tgtgctggtt 68820
ggcctcctgc caggaggtgg ctctctccag aaagcatcaa ctgtagtagt gtggagaggg 68880
gccagtggtg ggtggggccc taggactccc aagattatgc gtcctttgtc taccactact 68940
agggtgggta ggaaaggacc ctcaggtgag gacggtgcta ggcatgtctg agatcagact 69000
cttcttgggc aggtgttgct gtagctgctg tggaggatgg gggtgagatg cccaggtcat 69060
tggagttgtg tgcctaggag aattatggct gcctctgctg agtcatgcag gttgtcaggg 69120
aagtggggga aagccggaag tcacaggcct catccagctc ccatgcaaac tgaaggggcg 69180
gtctcactcc caccatgtcc tgcaaacagc cctccatctg gaaacagcaa acagtgtgtt 69240
tccagatgga gggccagacg aattgaaaac ttgcctgagg ctttccacct cccagctgcc 69300
aaagaaaagg gctttagttc ttcctccgcc tgtgaaatct gcaagccaga ttcacaccct 69360
cccctgggtt ctggcccaga ggcttctcac cccattcaaa ttgttacaaa gttcagctag 69420
agaattcctt ctccctgtgg agctgtggag ttttacccct tgctttctgg ccaccctcct 69480
gatggatccc tgtggtgcca ggcaggaatg ggctgcttgg ggatccagtg agctcccagg 69540
gcctttctgc tgcttcctct gaccctgtat tttgctcggc tgagttttgt aacttgactc 69600
agctccagga aaagtcggaa acttcttcca caaacaggcc ttcagcttct ccagtggggg 69660
tgtgtgctca ggagaagcgg gtctcccttt cccacttcca cagctggggc actcacagta 69720
tttggggtgt ctcctgggcc ctacaggagc aagtctgctt ccttcagagg gtctgtggat 69780
cctctcagga ttgctggttt gttctttcag tggatctgca gctaaaattc acaatgcaag 69840
cctccacatg ctgctctgtc cggagctgca atctggctat gcctcccgtc tgccatgatc 69900
ccgaaatccc tctccaattt ttatattttt agtagagttg gggttttgcc atgttggcca 69960
ggctggtatt gaacttctga cctcaagtta tctgcccatc tcggcctccc aaagtgttgg 70020
tgtaccagga caagctgcag acaaaacctc tgagacacca agttaaagaa ggaagggctt 70080
tattcggccg ggaacttcag caagactcac gtctccaaca accgagctcc ctgagtgagc 70140
aattcttgtc ccttttaagg gcttacaatt ctaaggggtc tgtgtgagag ggtcatgatc 70200
aattgagcaa gcagggggta cacgactggg ggctgcatgc accagtaatt agaatggaac 70260
agaacaggac agggattttc acaatgcttt tccatacaat gtctggagtc tatagataac 70320
ataactggtt aggtcagggg tggatcttta accaagccca aggtgcagcg ccgggctgtc 70380
tgcctgtgga tttcatttct gccttttagt ttttacctct ttctttggaa gcagaaattg 70440
ggcataagac aatatgaggg gtggtctcct accttattgg ggttacaggc atgagccacc 70500
gtgcccagcc aagaccctga atttaaatcc tgactctgtc attcattagc tcctgacctc 70560
aagtatgcca ttaactactt tgaatcctgg tatccttgct gtcaaaggag atgagagttc 70620
tgacacttat aggtccactg taagacataa tttttaaaat atctgtaaaa actgggccgg 70680
gcatggtggc tcatacctgt aatcccaata ctttgggagg tcaaggaagg cagattgctt 70740
gagcccagga gtttgagacc agcctgggca acatgatgaa accccgtctt taccaaaaat 70800
tcaaaaaaat ttagctaggc attggcacgt gtctgttagt cccatctgct cgggaggctg 70860
aagtgggagg atcactggag cccaggaggc agaggttgca gtgagccgag attgtgccac 70920
tgcactccag cctggcagag tgagaccctg tctctacaaa aatgtaaaat aaacttaaaa 70980
aatgtcaaag catgataaag tacaggacat aaagaccatg attaatctaa cacccatcac 71040
tacaatcttt gcctctgttc tggcattccc ctatgtgtct tcgcatcacc ctccttctgg 71100
gtgactcctt tatgttgtat ttttttgttt ggtttggttt ttttggttag tttgttctga 71160
gacagagtct tgctctgttg ctcaggctgg agtgtagtgg tgtgatcttg gctcactgct 71220
acctctgcct cccaagtttg tacctcagcc tcccgagtag ttgggattac aggcatgcgc 71280
caccatgccc tgctaatttt tgtattttta gtagagacgg ggttttgcca tgttggccag 71340
gctggtctca gactcctggc tcaagtgacc tgcccacctc agcctcctaa agtgttggga 71400
ctacaggcat gagccaccac gcccggccac cccactcatt ttgaatgtaa gtagattacc 71460
aattctgaaa tgggttttga gcagtagcaa cattgacaca aatttataat attttcaaga 71520
agatacttcc ttcaatatgt aaaatgcaac agatatcatt tgcaatcata catatgtaca 71580
tatatacata cacacatata atatatatat ctcaccatgg aataagtctg agctgaaaca 71640
tgcattactt aaaaaagaaa acacttacag acaggatctt gctccgtcat ccaggctgaa 71700
gtacagtggc acgattgtgg ctcactgcag cctcgacctc ctgggctcaa gtgatcctcc 71760
cacctcagcc tcccaagtag ctgggactac aggcatgagc tacctcgcct ggcctgcatg 71820
atctttatga agaatgtcat agaaagtttt tgaaagcgat taaagttcac ctaaacaagc 71880
agggatctat gctgtatgcc atgctattat tggggaggaa aaaaattctc ctctacattc 71940
ttagggtctc tggagatctg gaactaagaa taaaactgaa ataagacaga ttaacagaag 72000
aaaatcattc aagttttgct aaattttcag tgtacatggg tactttcaca agagaatgaa 72060
ggcctaaaga agtcactaaa gtagaaaaac aatacctttt tttttttttt ttttaagaca 72120
aggacgggtg cggtggctca tgcctataat cccagcactt tgggaggcca aggcgggcag 72180
atcacaaggt caggagattg agaccatcct ggccaacatg gtgaaacccc atctctacta 72240
aaaatacaaa aaaattagcc aggtgcggtg gcgggcacct gtagtcccag ctactcagga 72300
ggctgaggca ggagaatggc atgaatcctg gaggcggagc ttgcagtgag cggagatcgc 72360
accactgcac tccagcctgg gtgacagagc aagactctgc ctcaaaaaaa aaaaatgaca 72420
agacaaagcg gcttggatta tagggagtaa attgtggaga agggactagg acatacatgc 72480
ggagcaaaat tagaagataa ggattatttt tagtcgtgtg tttatacaga tccattggaa 72540
ccttgattca cagtcacttg tgatgacaat gttcttctct tcctggtaaa gggagggtgc 72600
tttcttatgg gaaattgtgt ggcctattct tagatagaaa agggcaggtc agagagtctt 72660
tcctgcatgt gctatttctc aagtgccttc aactcaaaat aattaatact ccaaagcagc 72720
atatttgggg tgacgtgttt ttaattcctt catttcccct gctgcagact ttcctagaag 72780
tttcagatat taaaagctga gttggtggct atggagataa gagccagatt cataacgagg 72840
gatctacaaa tggggagaag aacatagata agaataggaa tgaataagct gaaaggaaca 72900
ggtctgagca catttcccct tatcctgtta aacacacatc ccattgctgg gacaaggtca 72960
gtacagcaga aggaatgcac ctcttttgtt tgatggagag tgtttagctt gttctttaac 73020
aggtgcagct taggcactat ttgtcccagt ggatttacac agaccagcct tatccaccaa 73080
tgatcacaca aactcctcct tttgaggctg tcagtgtgtc cagggtgcaa caattttgga 73140
atacagcaga agctaagcta ttaacttctt ttgttgtggt tgctgttgtt gttttgagac 73200
agaatctcac tttgttgagt gcaggctgga gtgcagtggt gcgatcatgg cttgctgcat 73260
ccccagcctc ctgggctcaa gtgatcctcc cacctcagcc cccagagtag ctggaactac 73320
tactatgccc tactaatttt taaacttttt gtagagacag tctcactatg ctgcccaggc 73380
tggcctcgaa cttctgggct caagtgatcc tccctcctca gactttcaaa gtgctgggat 73440
tagaggcctc agccaccatg cccagattgc tttgacatct tgaggcctta ctgatccagg 73500
agagactgcc cctccctggg ccagctaatt cctacagaga ataaatgatt tgcttatggt 73560
gtgccttcga tgggccaatc aaccgattcg tatctccaac ataactttac cagccacctt 73620
tcagcaggtt cctgccccaa atcactccag ggacaggtac caggtaacaa aggaccactc 73680
ctataaccca gagcccatca caattattaa aactagccag tcctaagtct gtttaccctg 73740
tcttgccttc ttgcaaaaaa cacagtaaag ccttttgtcc accttttgcc ctcactcatt 73800
ctgccacctg actgaccctg gtgcttcccc atggccctgc atggtgtggc atgtcccccc 73860
tattgtcttg ggaactgtga gtaacaaact ttcttttaca tggctatcac ctctgatctg 73920
tggcctcgcc atacctgaat aaagccaaaa tcccaggtac attttaaaac aagtctacaa 73980
gtcaaggaat accaaggatt tccagcaaca ccagagaggc cggcccacgc ccggcctgac 74040
tcttactgtt aatcactagt tttgcatttc tcttgaggaa ctagtcagga ggagaaaacc 74100
tagacagcag ctctcctggt caagacccag tcattcagaa gcctaaatgg catgcctagg 74160
tgcttggctt taaactgtta gcaggtatgg agtgctctga ttgtgtttta gaataatatt 74220
ctgggctggg cacggtggct cacgcctgta atcccagcac tttgggaggc ggaggcaggc 74280
ggatcacgag gtcaggagat tgagaccatc ctggctaaga tggtgaaacc ccgtctctac 74340
taaaaataga aaaaattagc cgggcgtggt ggtgggcgcc tgtcgtccca gctactcggg 74400
aggctgaggc aggagaatgg cgtaaaccca ggagtcggag cttgcagtga gccgagattg 74460
cgccactcat tccagcctgg gcaacagagc gagactccat ctcaaaaaaa aaaaaaaaaa 74520
aaaaaagaat aatattctgg ctgctgctgg aaggcagcca gggaggaagc aaggacattg 74580
gatactgcag taatcctggc caaaggcgac ttgtgaaaag gggtgggcaa ctgaaggggc 74640
aggagaatgt ggcccctctc agtctggaga gagctgcagc actaggccag gaaggcactc 74700
ttctgcgggg ggataggtct taaatgggga ccatgggatt gggtgcagtg gctcatgcct 74760
gtaatctcaa cacttcagga gtctgaggca ggaagaccac ttgagcccag gagttggaga 74820
ccagcctggg caacatagtg agaccctgtc tcaaaaaaaa aaaaaaagac aatttaaaaa 74880
ctagccagat gtgatgcctg tggtcccagc tacttgggag gctgaggtga gatgatcact 74940
tgatcacttg agccctggag gtcaaggcta cactgagcca tgatcatccc actgcactcc 75000
agcctgcgca acaaagtgag actcttatct caaaaaaggc gacaggtggg aaccatgggg 75060
aaccagagaa gcaaagctat tctcaagttt tccagtaacc atcctcttgc caatggtctt 75120
tctctgaatc ttggatttgg gaataggatt ttcctgcctt gggaggctcc cttgggaaat 75180
gggagtgtca cagccacagg catcaaaagc cctagagttg cacttgaata gtgggtagaa 75240
agttctgtgc aatgccaggc actgttggaa acctctgggt agggcttaca gggccttagc 75300
catctgtggg aggcattagc tggagtgtaa taaatatgac cctcaggact ggggagaaac 75360
agctcattcc ttcatgaggt gtggggctgg cagccttgca ggcagccagc cacccttccc 75420
ctctggcctc catcctgggc actgagccag gcactgggta agaaggctgc tgcacctcct 75480
ttctcctctt cccttttaga gagaaagata cccccagccg aggatcccag aaaggcgaga 75540
gcagacgcgc cattgattgg cccaaagttc tccggagcca acctttgagc aaggagggcg 75600
tggctgggct ccagtctcct agtgaggtgc accactgcag gcttggtgac ccagccgttc 75660
ctgcccgctg cccccaacca gccgttcctg cccacagttc ctgttcccgg gcctgtcccc 75720
gggtttgtcc ccagggcccg tcccctgggt ttaggtctac cctccctcgc cccaccccat 75780
cgccctcgcc atgggtctgg agctgtacct ggacctgctg tcccagccct gccgttcagt 75840
ctacatcttc accaagaacg gcatcccctt cgagcttcgc aaagtggagc tgaccaaagc 75900
tgggctgggc aggcaggccc aggggatatt ggctgcggat ccccacctgt ccccactgct 75960
ttgcagtcgg gccgagaatg cagactaaca tgaagaatca gactctctgg tgtggaaact 76020
attacgtaat tatctatgtt attattttta ctttttggag acaggagcta actctgtcac 76080
tcaggctgga gtgcagtggc acaatcatgg ctcactgcag cctcgacctt ccaggctcaa 76140
gcgatcctcc catctcagcc cctcaagtag ctgggactac agatgcccac cgatatgccc 76200
ggccaattta ttattattat tattattatt attactattg agaggaggtc ttcctgtgtt 76260
gcccaggcag gtctcaaact cctgagctca ggcgatcctc tttccttggc ctcccaaagt 76320
gctgggatta caggcatcag ctgtcatgcc ctgctggttg tttattttaa aagaggcttc 76380
atctttgctt agtgaataca aagcattctt caaaatacag gttaagaggg ggcagagcag 76440
atgcagcagg agggcaggga aatggagtgg ggctagaata tcttcacccc atgtgcttgg 76500
aaaaaacaat gaaactacct ccttgtttcc taagtggagt agtccttgtt tacctctgcc 76560
tgggtctctt cccttcttgc ctcttctttt tttgacacgg tctcactctg cagcccaggt 76620
agagggcagt ggcatgatcc cggctcactg cagccttgac ctctcaggct caagtgatcc 76680
tcccacttcc tcctctcaag tagagtagtt gggaccacag gcacgcaacc acatccagca 76740
atttcttttt tttccttttt tttttttttt ttggtatttg tggagacggg attttgccat 76800
gtagaccagg ctgaccttga actccagagc tcaagtgatc ctcctgcctc ctccttctaa 76860
agtgttgaga ttacagacat gcactaccat gtctggccct gggtctcttc cacttttatg 76920
tatttcttgg cattcttctg ccttttcttt tcctgcatct gacttgctct atcccatggg 76980
gcttctcacc cagctgaagg ggctgctcct agaggctaga ggctggaggg ccctgtggca 77040
gggcagtggg ggaggccagc accctcaccc tgagctccag cgtttgctgg tctggctggc 77100
atacagagat gcagctggag cacaattcag tggaggaggc agggctataa ctaagcagta 77160
gggcagagag cagagccaga gccccctggg caggccagaa ggaggaacct gctcagctga 77220
ctcttcccaa ctgctgccct tggtacaacc agcttgagtc acctgctaca gaacaggggc 77280
tggacacaga agcagtctgt ggagggagaa tgggcagggg tgtgcatgtc cattcagtag 77340
aaggccagag aggcccacat gtaggcctat gatcagactg gagcctggct tgactgatgc 77400
cctctgatcc aaacaccagg cacctaatgc ggcctctagc actggcctag agtgctaaag 77460
tgagtcagac ctgggaaccc ccagtgggga gggagccttg agagtttggc acaagaaagg 77520
aagaagagct gagccaccgg gggctgagtg gctgaatggg gcagggattc ccagcaagaa 77580
tgaggttggt ctgagggttg ggaacatgtt tggctgggac tctggggtat tgacaggtca 77640
cccagcctgg ccagtagggc ctaaggcccc agctgggata ggaagctgaa tcagaggggg 77700
caagcctaga gcccagggag gaggctggag gggtctgacc tatggatgga actggctggg 77760
ggtggggtgt gaattacagc tccagggaat tgtccttgag ccatgcttca aggaactgga 77820
cacaggaaag gtggccctct gatagggtca gggtctgtga cccagtgact aacagagagg 77880
cttcctcttc cctgatgcct cccagatagg ggcctcagaa agctgctgct gggagtttgg 77940
agtgggggtg ggcagagaca tcagagaact cagggttctc cccactcacc tctctgatct 78000
cgcctgcagg ccagcagcac agtgatgcct ttgcccaggt gaatgccctg aggaaggtgc 78060
caggccttga aggatgggga cttcaccttg gctgagaggt aaccggtccc tgggctgctg 78120
ccgggccttg tggggccagt cagtcagctg tctgtttact gctgatggct tggatcatgg 78180
actgtgggcc ctgcaccagc cccagggtga agaatgggtg gaggcaggca gaggtgccca 78240
gtgttgacaa gggctataga agtcccctcc tgcattgtac aagtgaggaa actgaggctg 78300
gaggagggga gggacttgca caaggccaca gggcccagtc tgcttccggg cctcctcacc 78360
gccagtcttc ccctccgaaa aaaagagagt gctaagtttc gaggagcggc tgtaccaggc 78420
atcttagtgc taccattttg cccacaaatc ctgtggggca agtgctgttt ccctggagag 78480
tcgagggacc actcagcgag cttcagtggc tatttgctaa acatccacca gggccagacc 78540
ccatttaagc cctggggctt gagcagtgaa cgtgtctacg tccttctcct catggagctc 78600
acattccatt aatagaaggg ggagctgaca gtaacccatg taaacatgga acatatcgga 78660
tgttgatgga tgccatggaa aaaataaaca gggtaagagt gggggtgtgt gtcattatga 78720
gtaggatgtt gagggaagac tccctggagg aggtggcatc tgagcagaga gctgtaggaa 78780
tgtgcaaggc atgaggggaa gggtgttctc actggaggga agagtgaggg cgaaggctcc 78840
aggtgggaca atgcatgggg ttcagactgg agagcaacgg gccagggtgg gtgcagtgag 78900
caggaagagc aggaggtcat gacagagggg agcatgggac actgggaagg ctttggctct 78960
taccacaggt agattgagtc gctggagggt tttgagcaga ggagaactgt gatctgactt 79020
acgtcataaa ggatccccct ggcaggggtg agaaagccac tgcagtcatc tggcatgcag 79080
cgctggagcc ccagaccaag gtggtggtgg tgcagggttg gtggggattg tggtcagttc 79140
tgacttttat tgaaatctaa ctgcagggtt tgctgaaggg ttggaggtgg gtgggaaaaa 79200
tgaaagccaa aaccagcctc taggttggtg gctgcatggt ggcgtccttt accgaggcca 79260
aatagtgcct ccttggtgga gctggattca cattcagagc ctgggatcac tggatggtcc 79320
aacacccata gaggcatgat gagctctggg ggtcagactg cacaaccctt caccataggt 79380
gaacaggaga tgggacaggg tggggagcaa tgctgccatg tcacctggaa ggctctctcc 79440
ctgctggcct tgctcctggc cctgtccacg ctttggatat gttggggaga tatccaacat 79500
gctctcgttc ttcagactca cagtggatgc cccttcctcc tggaagcctt ccctgagccc 79560
ctgctgaagt tcatgacatt ttgtttctta cttgctgccc ttagttgaga tgatagtctg 79620
gccccgtgta gggctgtgtg accctgggca cttacctaac ctctctgagc ctcacatccc 79680
tcctctgcat catggggata gtgaaaatgc cccagaaaat ggctgaagag tgttcagggc 79740
atgttttctg cccctgccat gtccccccag tgtggctagc ttgctgtgta tgaactgcat 79800
gtacaaagca cctgaccact ggtaccccca ggacctgcag gcccgcgccc gtggggatga 79860
gtacctgtca tggcagcaca tggccctgca gagtagctgc tgctgggcca tgtggcagaa 79920
ggcgagccat ggagggcagg gacatctccc aagatgccaa gggatgctgc tttcacttta 79980
caaaatcctg tttagacctg agaccaaatc ctggaatgcc agagactgcc taattcaccc 80040
aacttgtttc ctaggaggga aaccaaggac cagagccaag gaaggattcc cctaatatgc 80100
cttaattcca gagctatgag cagttaatcc tatgctcttt tgcatactcc aggttgcctg 80160
ggaatcttgc ctttaggggg agaaaataaa caaaccctca ctttgctaca cacataattc 80220
gtagatggag aagtatgaag tgaaaaaagt caaactagga agaagtgagg agacatttca 80280
ataaacgttt gtctgatgtc tcggtatgaa aggactttct aaccctcaga acagtgggag 80340
aactcaacaa agaaataacc actacatgcc agctgcggta gcatctgtaa tgagcacacc 80400
tgtaatccca gcactttggg aggccgaggc aggcagatca cctgagattg ggagtttgag 80460
accagcgtga ccaacatgga gaaaccccgt ctctaccaaa aatgcaaaat tagccgggta 80520
tggtggcgca tgcctgtaat cccagctact cgggaggctg agtcaggaga attgcttgaa 80580
tctgggaggc agagattgca gtcagctgag atcacgccat tgaactccag ccttggcaac 80640
aagagcaaaa ctctgtctca aaaaacaaac aaacaaaaaa acatatacaa ggacatgttg 80700
tgtaagaaaa aatggattaa acataaaaca agcaaaaaac cgggaaggat cttgataaaa 80760
gatatgtcag aaggaggtaa tgttctttaa gatgctctta gaaaccctta agagaggcct 80820
ggtatggtgg ctcacccctg taatcccagc attttgggag gccaaggtgg gcagatcact 80880
tgaggtcagg agtttgagac caccctgggc aacatgatga aaccccatct atattaaaaa 80940
tacaaaaatt agttgggtgc ggtggcacac tcaggagact gagacaggag aatcacttga 81000
acctgggagg cggaggttgc agtgagctga catcatacca ctgcactcaa gctccactta 81060
gagcgagact gtctcaaaag caaacaaaga aaaataagag aaacccatca gacaagagac 81120
aggaaaaaaa tgagtcatag ctcactgcat tctagatctc ctgggctcaa gcgatcctcc 81180
tgcctccgcc caggtagcta gaactacagg tgcacaccac aacactggga ttcttttttt 81240
ttttcttttt ttttgtagaa aacaacgaag ggaatcttgc tatgttgcct agcctggtct 81300
tgaactcctg ggctcaagca atcctccctt cttggcctcc caaagagctg agattacagg 81360
tgtcagccac tttgcgtgac cacaatttca atcatattta aaatgtacaa ttcaagtgat 81420
tttgccacac taaccacaga ctcatgttta agccacacca acggatttcc atgttttaaa 81480
taacatattt tgccaccccc tagtggacag tggctcacca ccggcacaag gggctacata 81540
ggcagtcaat gggaaccagg cggggacggg aaatgtcgtg aacccaacgg tccctgccca 81600
gccccacccg cgtccgcaac gtgtaaccac atctcctgac tgcccaagag aagcgcagat 81660
ttccatactg aacatgaaat tgcctgactt cgaaatggtg gcaaatcatt aaaaaaaatt 81720
ttaaactcac tttgcattgg ttattagggt cgaacttggg gaagacccct acaggtgtgt 81780
gtgtgtctgt atgttagggg tgttcagacc tttaataggg ctagaaacca ggcaaccaca 81840
ccgcggaaag cttgaggaga gggaaatcca ccagacgcca agcaggaggg tcagggaaga 81900
gacagggtgg ggccactacc gggttaaaga cttgtagtgg gtggggctac acgtagggcc 81960
gagacgaccg gatttccgga aatcagagcc ggcacacgtg acttttgttt gcagaagtgg 82020
gaaggtaccc taggcagcca atcggggagc gctgagtctc tgtccagcca atgagaagcc 82080
aggttgtttt agcgcctcgc ccctcctctc cggtccgcga gccttgggta tctccagctt 82140
ttttccgcca gagctgtttc cgttcctctg cccgccatgc cgttcctaga gctgcacacg 82200
aatttccccg ccaaccgagt gcccgcgggg ctggagaaac ggctgtgcgc cgtcgctgcc 82260
tccatcttgg gcaaacctgc agacgtgagc gtgggccggg cagcactggg cgaggggaag 82320
ttggtgggcc aggggtccgg ccttgtccct gctctgcctc cgcaacagcg accccgatcc 82380
ctttccccag ggaccacccc ccaccccatt ccgcaggcca agctctgact ttccgtgctt 82440
cacgatcccg cggctccccc tccgcacgtc tttcccttgt cgccctcccc agtcatgacc 82500
cggacgtgac cttcagggac ctcggcctgt actgggatcc ctgtccccgc gaacactgcg 82560
cgtttcggct ttcgcgcgct cgggtcggga ccccagacgt agcccgactg gctccagctt 82620
cgggtaaaac ttttcatgtt cccctcagct tgtgaacgtg acggtacggc cgggcctggc 82680
cagggcgctg agcgggtcca ccgagccctg cgcgcagctg tccatctcct ccatcggcgt 82740
agtgggcacc gccgaggaca accgcagcca cagtgcccac ttctttgagt ttctcaccaa 82800
ggagctagcc ctgggccagg accggtgcgc aggggtagta ggcccggaat attattctaa 82860
aacacaatca gagtactcca ttcctgctaa cagtttaaag ccaaacacct aggcaggcca 82920
tttaggcttc tgaatgactg ggtcttgacc aggagagctg ctgtctaggt tttctcttcc 82980
tgaccagttc ctcaagagaa atgcaaaact agtgattaac agtaagagtc aggcagggcg 83040
cggtggctca cgcctgtaat cccagcactt tgggaggccg aggcgggcgg attatgaggt 83100
caggagatcg agaccatcct gactaacacg gtgaaactcc gtctctacta aaaatacaaa 83160
aaactagccg gacgtggtgg gcgcctgtag tccccgctac tcgggaggct gaggcaggag 83220
aatggcgtga acccgggagg cggagcttgc agtgagccga aattgcgcta ctgcacacca 83280
gccggggcga caaagcgaga ctgtctcaaa aaaataccaa aaaacaagca aaagaaaaca 83340
aaacaaaaac agtaagagtc gtcccaacgc agtggcttat gcctgtaatt ccagcacttt 83400
gggaggccga ggcaggcaga tcacctgtgg ttgggagttc gagaccagcc tgaccaacat 83460
ggagaaaccc tgtctctact acaaatacaa aattagctgg gcgtggtggt gcatgcctgt 83520
aatcccagct actcagaagg ctgaggcagg agaatcactt taacccggga ggcggaggtt 83580
gtggtgagct gagattttgc cactgaactc cagcctgggc aaaaagagca aaactccttc 83640
tcaaaaaaac aaacaaacaa acaaaaaaaa aaaacacaca cacacacaat ccaaaaacca 83700
gtaagggtca tgtcctggga ggctaactca gccgactgtt aggtcaccat gcttatagcc 83760
aagaaatgtg ggatgctgct gggccctgag cggtcagata aactactttc tctgataatt 83820
agaaggctac aggaaatgtt ttgttttgtt ttgttttggg tttctttgtg tgtttttaga 83880
gacaggatct tgttctgttg ccccaggctg gagtgctatg gctcaatcag agctcactgc 83940
agcctcgaac tcctgggctc aagcaatcct cccacctcag cctcccaagt agctagaact 84000
acaggtatgc atcaccacgc ctgactaatt aaaaaaaaaa tttcttttgg ttgcgtgtgg 84060
tgtcttggca cccgtaatcc cagcactttg ggaggtagag gtgggagcat ctcttgagca 84120
caggagtgca gggctagcct gggcaacatg gcaagaccct gtctctacaa aaataaaaaa 84180
gaaatgagct gggcgtggtg gggtgcacct gttgtcccag cttcttagga ggctgaggtg 84240
ggaatattgc ttgagcctgg gatgtcaagg ctgcagtgag ccatgactgt gccactgcac 84300
tccaggctgg gcaacagagt gagaccccat ctctgaaaaa atattttttt ggggagccag 84360
gcacagtggc tcatggtaat cccagcactt tcagaggtgg aggtgcacag atcatttgag 84420
gtacagagtt tgacaccagc ctggacaaca cggtgaaacc ctgtttctac taaaaacaca 84480
aaaattagcc aggtgtgata gtgcgtgcct ataatcccag cttctgggga gactgaggca 84540
agagaatcgc ttgaacctgg gaggtggagg ttgcagtgag ctgtgatcat gccattgcac 84600
tccagcctgg gcaacagagc aagacactat ctcaaaaaaa aaaaaaaaaa agtttgtttt 84660
gaaatcctgg actcaagcaa tccccccaac ctggacctcc caaagtacta ggattataag 84720
tatgagctac cacactcagc cacttttttt ttttttgaga tggagtctcg ctcggttgcc 84780
caggctggag tgcagtggcg cgatctcggc tcattgcaag gtccgcctcc tgggttcatg 84840
ccattctcct gcctcagcct cccgagtagc tgggactaca ggcacccacc accacgcccg 84900
gctaattttt tgtattttta gtagagacgg ggtttcaccg tgttagccag gatggtctcg 84960
atctcctgac ctcgtgatcc ccctgccccg gcctcccaaa gtgctgggat tacaagcgtg 85020
agccaccgca cgcggcctca gccacattta ttaatttcac tcttggcaaa catcaggggg 85080
aagctgaccc acacggcctg ggaagggggt tgtcttttgc atagagacca tgaccaggtc 85140
tgggacagag gaaagtcaaa taaatcacac attagagtta gaagcagagg ctcaggctga 85200
gcccaggttt attatccaaa atcaaaatga aatgcagtga ttaaaggaca caaggcctca 85260
gtgtgcatca ttctcattgt ggctttcagg cggctgtgga agacagggtg gggatggtgg 85320
cttcgggagg tgaggtgctc tgggacttgg gcaagtctta ggcaagccat tcctgctttc 85380
tgggcctggc tcccatgggc cattagaaat gaaaatgctt tgtggactgc tgaggacggt 85440
gcaagggtga ggtttcccag ctcaccggat catggccagc acccagggca tcagcttctg 85500
ctttatggtg gggtctgcag gtgggaagtc cttggccttc agaatgacct catgggcctc 85560
ctggaagagg tcctccccca ctgctgcctc cacgcgctgc cgccatgtgg ccagcttggg 85620
tcggccttcg aagacttggc agccagcacc cacgggctgt ggggaaaagg gtacagactg 85680
gggatggatg gttgtgaggg cagggatggg cagcatctga tttggggacc acagatctcc 85740
aggaggtgtt tgcacacaca cttaagcaca gtgccatagc ccggtgtggc agcataagca 85800
ggacttcagc aactagccag ggccccctgc ctagtgggtt cacctgccca cagcactcac 85860
atgcatcagc tccgtgatgg ctacgaggtc agctaaggag atgtgaggac cagtaaggaa 85920
ggccttgttc tggaggaact tgtcctcgag caactgcagg gtcacatcca actctgccag 85980
ggtggctgcc agtgtctggg gagatactgg ctcacccagg aaaacaggga acatcacctg 86040
gggattgggc aggcaaagtc gggagttact ggagtaggga gttagttttg atttgcattt 86100
ccctaatgat tagtgatgct gagcatcttt tctttttttt ttttttttga gactgagtct 86160
tgctctctcg cccaggctgg agtgcagtgg ggtgatctcg gctcactgca agctccgcct 86220
cctgggttca cgccattctc ctgcctcagc ctcccgagta gctgggacta caggtgcccg 86280
ccaccacgcc cagctaattt tttttttatt tttagtagag acagggtttc actgtgttag 86340
ccaggatggt ctcaatctcc tgaccttgtg atctgcctgc ctccgcctcc caaagtgctg 86400
ggattacagg cgtgagccac cgcgcccggc caagcatctt ttcttgtact tgttggccaa 86460
aatgataata ttctcaacac attgggttaa gtaaaatata tcaatttcct tttacttttt 86520
ttttttcttt tgagacagtg ttcagctctg tcacccaggc tggagtgaag tggcaccatc 86580
tcggcacact gccaactttg ccccctgggt tcaagcaatt ctcctgcctc agcatcctga 86640
gtagctggga ttataggcac aggccaccaa gcctggctaa tttttgtggt tttagtagag 86700
acaaggtttc cccatgttga ccaggctggt ctcgaactcc tgacctcagg tgatccactg 86760
ccttggcctc ccaaagtgct aggattattg gcatgagcca ctgtgcctgg actcttttta 86820
ctttttaaat gtgcctacta gaaaatttaa aattacgtat gtggctcact ttatacttct 86880
atcggacagc acttgactaa atgaactaaa ttccagagag gcataagccg ttcctagatg 86940
agctactttc attccaattt catttccttg ggcccaagca ggctgggaga aagactgtca 87000
caggcatggg cttttctgac tgagcagcag tttgggaact gggggcaggg atgaaaagca 87060
gagagatctt acaggcaaca tcgtagttgg tgattagatt ccaaagccat gctagactga 87120
gctagtgacc tgatctcaca ttcaagatat ttgaaaccaa agataaactg aaaccaacta 87180
aacctgcaac ccagcccacc tcctgattag cttagaagag aacagcctca catcctggct 87240
acctgacaca gggaagtgca agccctttct tgggggtgtt tattatgcaa ttcaatctct 87300
gctcttcttt tatcataatg tctggcacac aaggatacat catgaggccg ggcatggtgg 87360
ctcatgccta caatcccagc actttgggag gccgagatgg gaggatcacc tgaggtcagg 87420
agttcaagac cagcctggcc aacatggtga aaccctgtct ctacaaaaat acaaaaatta 87480
gccaggcatg atggcaggtg cctgtaattc tagctacttg ggaggctgag gtgggagaat 87540
cgcttgaacc caggaggcgg aggttgcagt gagccaagat cgtgccattg cacgccagcc 87600
tgggcaatgg agtgagagtc cgtctcaaaa aaataaaaaa taatgataat acatcatgag 87660
acatgcaaag aagcaggcaa atgtgaccca taatcaagag ggagaaaaca acaatcaata 87720
gaaatagacc cgtaggctgg gtatggtggc tcatgcctgt aatcccaaca ctttgggaag 87780
ccaaggagga cgaatcactt caggtggaga gttcgaaacc agcctggcca acatggtgaa 87840
acccggtctc cactacaaat aacaaaagtt agccaggtgt gctggtgggt gcctgtaatc 87900
ccagctacgc aggaggctga ggcaggagaa tcgcttgagc ctgggaggtg gaggttgcag 87960
tgagctgaga tcgcaccatt gcactcagcc tgggtgacag agtgagaccc catctcaaaa 88020
acaaaacaaa acaaaaaaac cagacccaca gaacacctac gtgttgaaat tagttgacaa 88080
agatttttag gtaacagtga taaatatgtt aaagaattta caagaaaaga cagataaaat 88140
ggatgaacag atggtgaatt tcatcagaga agtggaaatt ctaaaaaaca accaaatgga 88200
atttccagag ctgaaaaata catatctcaa acaaagaatg tattggatgg gcttaatagc 88260
aatttggcac aacagaggaa agaatctgtg aaggcaaaga caggtcagta gaaataatcc 88320
aaaactgaaa agaagagact ggtgcctgaa cacctttggg gaatgctctc gctgccgccc 88380
cccacattcc cagcctcacc ttatgccaca aggcccggag gcagcttctc cgcagagtcg 88440
tgtgctgcca tgccaggtac tcatccacac gggcacgggc ctgcaggtcc tgagggtacc 88500
agtagtcagg gaccttatat ttgcgcgtca ggtagagcag gatggccaca ctgtgggtgt 88560
gggggtcatg atgggtaagg gaagggcact gtgctgggta tttcatacat agtcgctttt 88620
tttttttttt gatacggagt ctcgctctgt cgctcaggct ggagtgcagt ggtgcgatct 88680
tggctcactg caacctccac ctcccgggtt caagtgattc tcctgcctca gcctcccaag 88740
tagctggaac tacaggtgcg tgccaccatg cctggctaat tttttgtagt tttagtagag 88800
atggggtttc accatattag ccaggatggt ctcgaactcc tgaccttgtg atctgcctgc 88860
ctcggcctcc caaagtgctg tgattacacg tataagcaac cgcacctggc cttttttttt 88920
tttttttttt tttttttttt tggagacaag gtcttcctca gtcacccagg ctggagtgca 88980
gtggtgcagt ctcagctcac tgcagcttca atttttatag gagtgcacca ccatgcctgg 89040
ctaccttttt tcttttttgt agagatggga tctcactgta ttgccaggct ggtcttaaac 89100
tcctatcctc aagcaatcct ccagccttgg catcccaaag cgctggcatt ataggcatga 89160
accactgtgc ccagcacact tctttaacct ttctgacaat ttgccaaagt attagcctca 89220
ttttccagag aagaaaacca ggcttagaaa ggcagagact tgccctaggg cacacagctc 89280
ttaatggcag agctgagaat ctttctgagg ctaataccca agcttttcct accaccaggc 89340
aaaatcccac aacagaatgt cttgccaaat gtttagagac agtttctgat cagagaacca 89400
gagagagctt ccaggagaac ggggcatcaa agctgagctt taaagaagct cagttctttc 89460
cattcggtat ccactctcat ctgctgggat tccgaccagc ctctgatggc ctgactgttc 89520
aaaaaggctt ccgtgtccct ttgttctttc tgtcattctt tctgccaaga atcagctcct 89580
atctaagctg actggggtag tttggcttcc taaagtacca ctcaggaagc actcctgcag 89640
ctgcaggatc ctagaacaac agaacagaca aggccccctg gcactctcct ccttccctgg 89700
gttttgaggg ccgccatggt gatgagatga gcatggacct ggaaggacac aggatgtgtc 89760
catttggaga cacagaaaag ctttcaggaa cttggggttt cccaaaggtt gggcatgcag 89820
gccaaaaggg cagaaaacct ctgtatctgc cacgggccta tattctcttt taagctgggg 89880
ctacacacgt gtcccagctt aacgactccg ttgtctgtgc agggtgccca ggagctcctt 89940
ttgctcccag ccaattgttc aagccacctc tgaggagggc tgcaggtctc tgtgagccac 90000
agacctgaag gtgagagtag tgacagaggc agagattgca cattgactct ggctggtacc 90060
tgggggtcac aacgctgagt gacacaagga ctcaggacac ctggggcacc agggaggaag 90120
acgggccctg gcagaatgtc agaggaattc atcggaggtg cgtccatcct gagatttcag 90180
agatgagctt taaagcacaa actccaggtt cttgttaggg gaagggtgac tctggtgaca 90240
aaaggggcag aactggggaa agattggctt ttcctgaaga ggattcaact tcttgggacc 90300
agctgtgatg taagacgtcc atatctggtg gctcttccat ggggaagacc catcctggga 90360
agctgcagcc cagagatgga gccaccgggc agcccagatg ccactgagcc atcgccacac 90420
aagcttggcc agaggcctgc cccacggtgg gcaaagctgc catcgtcctc tggcctaccc 90480
tgtctcaagg atactctcac cactcaccag caaccaaagc actggtgtgg aagtaaagta 90540
gatatttgct tttctaatca tcagggtgaa ctggaatagc aggaaggcaa gggcctgggg 90600
tccagtactg actctctgca ggatgcgggg cacagagact ctctgcctct gaagacttta 90660
gtttccttgt ctatgaaata gagggttaga acaggatggt tccagcaggc ctggcagcag 90720
tcctagggtc ccagttacct ctccgtcaag gtgaagtccc cgtccttcaa ggctggcacc 90780
ttcttgaggg ggttcacctg ggcaaaggca tcgcttaagt gctgacctgg gaagagtcca 90840
gcatggtggg tggtgggagg agaacgctga gcccgccatg gcctgtgccc ctccctgcaa 90900
tgctctggcc ccagtttcac agttcttctt cccaccagtc tgcactcctg gttggcaaag 90960
actggaaggg cccctgcggg aggaggagga gaccactcca ccggctggta caaaatagac 91020
aacacccctt gctaagtcct gtaggcacag cctcctccca caggctgccc ctctgcaaca 91080
gagctcactg tgcagggtgt ggttcctgga cctttgcatg caccctaccc atctcctctt 91140
tccactattg ccagcttcat gaactcacaa tcagagcacc ccaagctgca cgataggtca 91200
cctgagggga ctgactacct tgtcccttcc accattaatt ctaaggcagg gtcatgaaac 91260
acagagaagt ggtctttttc tggtatctac ctacttagaa gcaacttcta ctggccccac 91320
catgtccctt tgttcaccct gccctgggca ctgtggagct gtgtgtagca tacctgctac 91380
caccccctgt gccccaaact tttattcccc ttacagttgg ggtctcacta tgttgcccag 91440
gctggtcttg aactactggc ctcaagtaat cttcctactt cggcctccca aagtgctggg 91500
attacaggtg tgagccacca tgcccagcct gctaccctct cttcaccctc ccttcagact 91560
gttccttcac caggaaaacc ctcctctgtc tcctgggaaa acacacttcc ctctgcacca 91620
ctacatttga caaccagctt ggacgtcctc ttgtccccca ttccctttcc tctactccac 91680
actctgcaga ggaagtcctt gcgcaggaca gtgggcagtt tgcagaagag attatgtgga 91740
gaagtttcag gcagagggcc agccatggag tgggagccct gggcagaagg cttagccaag 91800
tagagtcgcc ttacttgccc caccagccat acctggattc tgcttccaac ccctcctact 91860
accccaccct gcactgggcc tgggcctggg cctgccagct gagtcctcaa gggccttgga 91920
ggcatgctag agaaacaaaa cagggtgtag ggctcatcaa gccccagggt ccccctgctg 91980
cttccagcat cagcagcctg gggcaaccta cctccccagc aggaacaaga ggcagaggac 92040
aaggtttcgg gcggcagagg ttgcagtgag ctgtgatcct gccactgcac tccagcctgg 92100
gcgacagagc gagactccgt ctcaaaaaca aacaaacaaa taaacaaaat gccttgggcc 92160
aggcacagtg gctcatgcct gtaatcccag cactttggga ggctaagccg gcagatcatt 92220
tgatgtcagg agttcgagaa cagcctgacc aaaattgtga aatcccatct ctgttaaaaa 92280
tacaaaaaaa ttagccagac atagtggcac acatttgtaa tctagctact caggaggctg 92340
aagcaggaga attgcttgaa cccgggaggt ggtggttgca gtgattcgag atccgtgcca 92400
ctgcactgca acaaagcaaa agcaagactc tgtctcaaaa aaaaaaggac aaggacaagg 92460
tcagaggcaa aacaggagga acagaggaag gaagggacaa agagggaccc caatgcagac 92520
tggacgagtg cagagggcag ggatggaaag tcacgtcctg agctgtttgc tgttgcagaa 92580
tctcagcagc tgggccataa agaagggact tgacgctgtc ccagtctcta cacttggcac 92640
ccttgacctc tctcatccca cctttcccta ggccgaggtc acacctgctt tcattttgtt 92700
tttttttgtt tttttttttt aagacggggt ctcgctagat tgccctgacg ggagtgcagt 92760
ggctattcaa gggcaccatg atagtgcact gcggcctgga attcctgggc tcaagcgatc 92820
ctcccacccc agcctctcga gttgctggga ctacaggcgt gtgcccctgc acccagctgc 92880
gcccgttttt gtcctcctgt ctggcttctc ttcatgatcc ccaccccttt attcggcctc 92940
aggggaaccc cgtggtctat tccgtgagac cgagcagagt tttggggagc cccgactctt 93000
ggatctccct gctggctcac ctttctctgt tcggtctcca aaccagacca gcaatggccc 93060
caggaagaag gtggcgctgg gagaagttcc ctaagctatg tatgcctacc tgtccccttc 93120
ctgacttttc ggttccccaa acccgaggct ggacctacct ttaatcagat ccacgatgcg 93180
cagctcgaag ggaatgtcgt tcttcttggc aaagatgtaa acagcgcggc agggctggga 93240
cagcaggtcc aggtacagct ccaggcccat agtggggacc gaccgacctg accggaaacc 93300
agagggagtc agcaaactcc agtgccagga ctcgggtctc ggacctcaga ccacgcccct 93360
gcggctacgc accccgaccg ctgtgcccca ttgggcggca cggacgcctg ggacactctt 93420
tagtccaatt gcgggcggcg ggcggcctgt ctcagggctg ggcccgcctg gctcctgtgg 93480
gatcagggtg ggtggccaag agaccgttgt ctctcagggg gacgcacaga gaggccccag 93540
tcccatccct ttcgtgcctg gctcgctcat ttcacttagc ataatgtctt caaggtgcat 93600
ctgtgttgaa gcgtgtgtca gaatttccca ccgtccgcat tttagaggag gagacagaca 93660
ctgaatgatt atagacccag aaagtctggc tccaaagtcc atcctcttgg ctcaacactg 93720
cagagtcctt ggtgccagca agtggcaaag cctcagggct tacgttcagt gttttgtggt 93780
cactcgcttc actgcagtcg cactccctgg aatgtcacag ccttggtctt cctgcccctg 93840
ccagcacagt gccatctatc gcattccctg ggtttatttc ttcctttctc caggcctgtg 93900
tttctccatc tttaaaatga cagggaagct agagccctcc agagtccttg caagtccacc 93960
cccctggatt catctgctga gccgtaagcc caggcctgcc ccttgctgcc atggagcctg 94020
tcaaatagta tcagggtcac atgtcatttt ctaaaacgaa tcccactgaa agtgtgtcag 94080
ccaacgcata atgttttcta gagaataagg aacatttctg caaataaagt ggaagtaaca 94140
actgttttat tttatttaga tagagtctca ctctcttgcc cagggtagaa tgcagtggca 94200
tgatcacagc tcactacaga ctccaactcc tgggctcaag ccatcctccc atctcagcct 94260
ccccaagtag ctgggactac aggtgcgcac aaagacgccc agctagttaa aaaaaatttt 94320
tttctgtaga ggcagggtct cactatgttg cctaggctga tcctcccacc tctgccacct 94380
gctgggatta cagatgtgag ccactacaac tggccagtaa taatgatttt taactggaaa 94440
aaaaattaag gaaagatgag aaattcagat ttccatgtta aagttttgaa aggaggtaga 94500
cactcagaga aggcaatggc tgccttatcc tcacctctgg ataactccag tcagaggccc 94560
aaggaggagc tgtcaggatt ctggttcaag gctcagcctg tcttttaagt tcaaaaccta 94620
atttggggcg ggagtggtgg ctcattccta taaccccagc accttgggag gctgaggccg 94680
gcggatcacc tgagttcagg agtttgagac cagcctggcc aacttggtga aaccccatct 94740
ctgctaaaaa tataaaaatt atctgggcgt ggtggcatgc acctgtaatt ccagctactc 94800
aggaggctga ggcaggagaa tcgttgaact ctagaggcag aggttgccgt gtgccaagac 94860
tgcgccattg cactccaggc tgggcgacaa gagagtaact ccaactaaaa aacaaaacaa 94920
ctaatttggt ccacgtttca ggattgatca ttttctttgg gtctgactca cataaaccaa 94980
ctcacaaagg ctgtatttat taatttatag aaacaagata aaagtgagag tgtgtgggtt 95040
ggttcgtttg tttgtttttt gactttgccc ctctctgtca ctcaggctga agtgcagtgg 95100
tgcaatctct gctcactgca acctctgcct cctgggttca agcgattctc gtgcctcagc 95160
cacccgggta gctgggattt caggcatgtg ccaccatgct cagctaattg tatttttagt 95220
agagacaggg tttcaccatg ttggccaggc tggtcttgaa ctcctggcct caagtgacct 95280
gccagcctcg gcctctcaaa gtgctgggat tacaggcgtg agccaccgtg cccggcctag 95340
aaagaagaga aaagtcattt agacagaatg ctcccaacca ttgctcagtg ctctcttggc 95400
agtgtagtct cctaggaagc ctcgtgggtg ttactggcca ggaccaggtc cgttctgccc 95460
atgcacagta aattaatcac tgtgacatga gttttgcaaa agactaaaga tttattcaca 95520
agggtgccaa gcaaggaggt aggataatag ctctcaaatc cacctccccg aaaataaggc 95580
ttagggataa gccttgcttc caagcaataa agcacagacc ccagtcacac tcaaggggag 95640
ggggctatac aaggatgtga ataccagtct gcctgcctcg tgggcctaca acgcagaagg 95700
gccatgcact tggtttaaaa ctttgttgtt gctgtcttga cattcttaac aatttaaaaa 95760
taagggtccc cacattttca tgtgtagcca gtcctgtcca aggagtccaa agaggtcatg 95820
agggtggaca ggtcactgtc tcctctgggc agtgatgggg ccactagcag ttgttctgta 95880
gtctcctagc ccctacccca gggccacacc ttccccatgg tctctcacat tctttttttt 95940
tttttttttt ttttttttga gacagggtcc tgctgttgcc caggctggag tgagtgcagt 96000
gctgcaaact cagcttacta tagcctcgac ctcctgggct caaacaatcc ttctgcctca 96060
ccctcctgtg tagttcggat cacaggcaca tgccaccatg cttggctaat tttttgactt 96120
tgtagagagg gcatcacact ttgtttccca gggtggtctt gaattcctgg gctcaagtga 96180
tcctcccacc tcagcctccc aaagtgctag gattataaag atgtgatccg ccgccccggc 96240
ctctctcaca ttcttggcat ttaaagttac cgtcattgac aaggaacatg ggtacactcc 96300
aatttactca agaaaaaaac tcttttctcc attgaagaga tatgtggcct ggctgcttcc 96360
tactctagtt gaggtcttct ttttgtttcc tttttcttct tctctcttgt tttttagaga 96420
cgaggtcctg ctgcgttgcc caggctggtc tcaaagttct agcctcaaga gaccctcctg 96480
ccttgtcctc ccaaagtgtt gggattacag gtgtgagcca ctctgtccag cctcaaactg 96540
tggtcattag atcagcagca gcagcagcct tgcctgggag cttgttagaa atatactaac 96600
aggctcccac ccccaaaccc agacctactg aatcagacag agtctgtatt ttcataagat 96660
cctcagatga tccaaatgcg ccttaaactt tgaggagctt tgatccagaa cctgggctgg 96720
aggcccctgg gggccactct gttcctcaca gactgcaagg tatgtttttt tcttgcctct 96780
tctctctgtc ctaatgtctc cttctctctg tgtctgaccc tgaggaccct atgacatctt 96840
cccaggggag tttccactgt caaagagctg attctctctc tctttttttt ttttttgaga 96900
cggagtttcg ctcttgttgc ccaggctgga gggcaatggc gtgatctcgg cttaccgcga 96960
cctctgcctc ccaggttgaa gcgattctcc tgcctcagcc tcccgagtag ctgggattac 97020
aggcatgcat caccacgccc agctaatttt ttgtagtttt agtagagaca gggtttctcc 97080
atgttggtca ggctggtctc gaactcctga actcaggtga tctgcccacc tcagcctccc 97140
aaaggcatga gccaccatgc cctactgcca gaggcctgat tctctagaat ctgatgggct 97200
cttctagaca gcaatctcta cctgggctct tgttgaagaa gccaatcagc tcattggttg 97260
gaggtcacat gatataaaac atggccatct tccaagccct atgtgtggac aacgagatag 97320
ggacaagtag gcatatcacg ttctgatacc tggacccaca tctgtcgctg ggcccacgtc 97380
tgtcctcagg cccccaggat atacttttat ggggagtttt cctcccttcc cccaatgtgg 97440
tcactcataa ctttacaccc aacagtcaaa caggagtgga agctacagag tttcacgcaa 97500
acaataggct aggctgtggg actgagtaaa aggcactgga ctgtggtagg tcttactcca 97560
agatggccaa atggaagggc ttttagcttt ctttgttttt gttttatatc ctgagacaaa 97620
atgttcaatt tctacacttt agaataaaaa gagccatatt ttatgtttca tattttttgt 97680
atgcttcctt tcatactctt ttgaacaatg caatcatatt ttcattatga ggtataattc 97740
acataccata atatccgctc tttcaaaata tactttaaaa ttcaattggt ttaaagccaa 97800
ttcattggtt tttagtatat taacaaagtt ttgtcaccat cacaattatc taattctaga 97860
tcattttcat cacccctaaa agaaactctg tacccattaa acagtcactc cccatttccc 97920
tcttctccta gcccctggta accattcatc tactttctac ctctgtggac agtaagtatc 97980
tgcctattct ggacatttca ttcaaattta ataatgaaat atgtgagctt ttatgactgg 98040
cttctttcat ttaacataat ggtgtcaagg ttcattcatg gtgtagcata taacagtact 98100
tcatcccttc ctgtggctga attatattcc attttatgta tacaccacat tctgtttatc 98160
cattcaccag ttgatggact tttgcattga ttctactttt cagctattgt gaatccccat 98220
gctgtgatga acattcacgt acaagttttt gtctggacat atgtcttcag ttgtcttggg 98280
tatacaccta ggaatggaat tgctggataa tataattcta tgttggccgg gcacggtggc 98340
acatgcctgt aatcccagca ctttggaagg ctgaggtggg tggatcacct gaggtcagga 98400
gttcgagacc agcctggcca acatggcgaa accccgtctc tactaaaaat acaaaaacta 98460
gctgggcatg gtggtgcctg cctgtaattc cagcctgtaa ttccagttac tcaggaggct 98520
gaggcaggag aattgcttga aaccaggaga cggaggttgc agtgagccga gatggcacca 98580
ctgcactcta gcgtgggtga cagagcaaga atctgtctcc aaaaaaaaaa aaaacaaaaa 98640
aaacaaaaca aaactaaccc atatatatat aatatgttat atatacataa taatatattt 98700
tatatatatg tgtatatatg tttaactttt ggaggaactg caagctgttt tccaaagcgg 98760
ttatacattt tacattctca gcagcagcac acaagggttt caatttcttc acacatcctc 98820
aacaatactt ttaatctgtc ttttctgtta tacccctcat agagtgtgtg aagtgatatc 98880
tcattgtggt ttgattggca atgtcttgat tatagtatct ttgtactaag ttttgaaatt 98940
gggaagcatg agtcctacag ctttttcttt ttcaaaatta ttttgaagat tctgagtcac 99000
tttaatttcc aaatgaattt tagggccaat ttgtcagttt gtgcaaacag gccaagtgga 99060
attttaatgc agactgtgtt gaatttgaca tcttaacatt actacacctt ccaatcatga 99120
acatgggatg tctttccatt tattaaggtc ttctttaagt tttcccccaa cttttaaatt 99180
atgaaaacat ttaaaatcca gaaaatgaaa ggactagtac aacagcacta atatatgcaa 99240
ccccagtttc aacttactgt tttttgaaca taagatacca acatcatgac accccaccta 99300
aggacttttt agcaaaacat tctaatttct ctactgggat tattgtttgt tgtcttcctc 99360
cacaataatg tgagcactaa aggtagagac ttttgcgtac tttgttcact actgcttcct 99420
ctgcaccaaa aacactgcct ggttgtgggt acttttctag ttttaggaag gagaagacaa 99480
gatataggtc ctgactctgg ttctgcctct cattcatatg ctactccgag gatcccttcc 99540
ccttgaagaa cttgtttctt catctacgaa atggggataa tactactact catcagggtg 99600
gttgtgagga ttaaacaagg taagagatgt aaaattgcat gttagttatc agatagtttt 99660
gttttcccat ctagctccat gagggagagg atttgctcta gtccgtccgt ctgggctctg 99720
ggcttctaat gggcagacac cttcctccat gatgtttgta gctgcaaagc ctggcacagt 99780
ccctcaagac aagctttttg acttgaactg ctgatggtct tccatgcaca attcaacaga 99840
aacccagtag catacacttc cttgtatgca cttgcccctc tttttacatt tattacacat 99900
tcattgctac ctaataatcc ccgatactta tcttttactt tatccaatta ttcaacagtt 99960
ttcaacagaa atacctagaa gagagctatt ccgacttcat tagaccctga gattctattt 100020
ttaacatttc ttccatggat tgttgaatgt ttccaatgat tgatccacct tcccttggcc 100080
ttatctgcat tccagatttg gttgtgtgtc catctctgtg ttgccagact tacctcacag 100140
acccagcttg aacacaagat gggtgcttgc tgaagagcag gactcattct gttgaacctg 100200
catgctttct caggtcagat tgcttggtgg tgcttcttta atttctttca acagcacatt 100260
gtagtttgct gtgtttaagt gttgcttttc tttggttaaa gttattctaa gtattttatt 100320
ctttttgatg ctattgtaaa tgaaagtgtt gttttaattt tgtttttgat cctttactgc 100380
aaatgtatac tattgatttt tgtgtattaa tcttgtatcc tgcaaccttg ctgaacttgc 100440
ttatgagctc taatggtttt taagtgagtt ccttaggagt tcctataatg tcatctgtga 100500
ctaaagatag ttttacttct ttctttccga tctggattcc accttgttcc tttttcttgc 100560
ctaattgccc taataaatag aagtagtatg aatgaacatt cttgtcctgc tcttgatctt 100620
gaggggaaac actcaatctt tcacaaggaa tgaagtatgg tgtttatagg tttttcatag 100680
atgctctttg tcaggctgag gaagttcctt tctagtcttc atctgttgag taattttatc 100740
ataaaaggat gttagatttt gtcaaatgcc ttctctgcat taggatgatc atgtgacttt 100800
ctattaatat gaagtgctat atggattggt ttttgtacat tgaaccacat ttgtactcct 100860
gggataaatc ctacctggtc aatgtgtata atcctcttaa tatgctgctg aatttgattt 100920
gctaatattt tgctgaggat atttacatct atatttataa ggatattgct ttgtaacttt 100980
attttcttat aatgtgtttt tctgtctttg gtatcagggt aatgctggcc tcatagaatg 101040
agttgaaaaa tatttcttcc tgttctattt tttagaagag tttttgaagg gttggtgtca 101100
attcttcaag cttttggttg aattcaccag tgaagctatc tggtcctgga cttttctttg 101160
tagggatttc atttattgat ttattgattt attttttgag acggagtctc gctctgtcac 101220
ccaggctgga gtgcagtggt gcgatctcgg ctcactgcaa gctccgcctt cctggttcac 101280
accattctcc tgcctcagcc tcccgagtag ctgggactac aggtgcccac caccacgccc 101340
catctaattt ttttgtattt ttagtagaga tggggtttca ctgcattagc caggatggtc 101400
ttgatctcct gacctcgtga tccgcccacc tcagcctccc aaagtgctgg gattacaggc 101460
atgagccacc gcgcccggct ttttggtagg attttaaaaa aattaatatt ttaatatctt 101520
tacttttaca ggtctattca aattttatct tccttcttaa gtccgttttg gtagattgca 101580
tgtttctaaa attttctcat ttcatcgagg ttatctcatt tgttcacata caattattca 101640
tagtatttct ttataaacct ttgtacttct ctaataccag tagtgatgtg tccattttct 101700
ttctttctgg tgtctggttt tttggttttt gttttttttg tttttttttt tgatataagg 101760
ttttcctatg ttacccagac tagagtgcag tggtgcaatc acagctcaat gtaaccttga 101820
attcctgggc taaagtgatc ctcccacctt agccacccaa tttgctagga ctacaggcgc 101880
gcaccaacac actcagctaa ttttttcttt cttttttttt tttgagacga aattttgctc 101940
ttgtcaccca gggtggagtg cagcagtgtg atcttggctc actgcaactc tgcttcttgg 102000
gttcaagcga ttctcctgcc tcagcctccc gagtagctgg gattacaggc gcctgccacc 102060
atgcccagct agttttttat ttttactaga gatggtgttt caccatgttg gccaggctgg 102120
tctgaaactc ctgatatcag gtgatccgcc cgtctcagcc tcccaaagtg ctgggattac 102180
aggcatgaac cactgggcct tgcctcctag ctaattaaaa aaaataaata actctttttg 102240
tagaaacaag tctcactatg ttgcccaggc tggtctcaat ctcttggcct caaatgatcc 102300
tcctgcctcg gcctcccaaa gtcctgggat tacaggtgtg agccattgca cccatcctcc 102360
actttcttgg tgaataattt gagtcttatt ttttcacttg gccattctaa ctaaaggttt 102420
gccaagtttt gttgatcttt tcaaagatcc aatttttggt ttctttgatt ttctctattg 102480
tttttctgtt ttccatttca tttatctcca ttctaatctt tattatttcc tgtcttctat 102540
tggtgttggg tttattttgc tcttattttt ctagttcctt aaggtgtaaa gttaggttat 102600
tgattgaaga ttgttcttct ttaacttagg tgtttacagc tataaatttt gctcttagca 102660
ctgcttctgc tgcatcctat aaatttgggc atgttgtgtt ttcattttta ttcattcaag 102720
atacttttta atttcccttg tggttttgtc tttgacccat tggttgttta agagtgtgcc 102780
gtttaatttc tatgtgcttg tgagttttct agttttcctt ttgttcttga ttttaaactt 102840
tattccactg tgtccagaga acaccctttg tatgatttca atcttcttaa atttgagact 102900
taggaccaaa tatatgctct atactggaga atattccatg tgctctttag aagaatgctt 102960
attctgctcc tgtcgggtag aacgttctgt tcatgtctag tgtgtgcaat tggtttacag 103020
tgcttttcaa gtcctctatt tccttgatgg tcttctgcag tttttctatt actgaaagtg 103080
ggggccaggt gtggtggctc acgccccgaa tccctttggg gattcagggt ggctcagcac 103140
tttgggaggc tgaggcgggc gatttgcctg agctcaggag ttcgagacca gcctgggcaa 103200
tacaatgaaa ccctgtctct actaaaatac agaaaaaaat tagctgggca tggcggcatg 103260
tgcctgtaat cccagctact caggaggctg aggcaggagg attgcttgaa cctgggaggt 103320
ggatgttgca gtgagctgag atcgcaccac tgcactccag cctgagtgac agagtgagac 103380
tccatctcaa aaaaaaaaaa aaaaaaaaaa aaaagtcagg tattgaagtc accaactatt 103440
attactgaca acgtaatcat atttaaccct ttacttttaa aattcttttt ggaaactgga 103500
aggatctgta accaccaccc acttccacat cagaccctat gcatacgcca actgtctgtt 103560
gctcagcagc tgtgatgttg ttccagttct ttattaggca aagaacagtt gtttttttag 103620
catttccttt aaggaaggtg gctcagatca ttgagccagc caggccctgc aggatgggct 103680
gcgattaggg tcacaagtac ttctcccgaa actcacagat tttcctcgtg accattggat 103740
ccaatgttga aaagtcccag tcggccaact gcattagtcg atcatgggcc tccctaaaga 103800
ggccacagcc aatattcagc tccacccgca tacgccactc agctagcttg gagctgttga 103860
ggaagacatt atagttggct gccatgggct gtggatagac aaagacgaag atgtggtcag 103920
cctggggacc agccccacct gggtcctctc ccacagcctc tggcccacgt ccctaacatc 103980
tacctatgga aagaattagg ctgctgaccc ccaaggcctg agcaaggggt cacagactat 104040
gtaatagatg taggtgggga tggaggatcc agaatccctc agagtcctca gtcactggtc 104100
ttcttgctcc aagccttctg aaccacactg agagggctcc tggcccaggc agctcccact 104160
tctccagcct gcgacctcgc atgaatcctg ccttcttcca gggaaacccc ttatcccagg 104220
tgtgtatatg cggatgaggg agagaactca ggttttttcc tccatgttta gactcccact 104280
atgatcaagc tcaggggcta agatgggaga cctggcgggc agtctaccct gcagtttcgc 104340
tggctcacta agacagtcta ccctgtagtt ccgtttgcac ccaagatctt tgggaagcca 104400
aagaaggggt gggtcgttgg tgaaatgtaa ggggtgggga cgaagtatat ggctgaaccc 104460
ttggggcagg ccagaatgat ttttcctggt gctggtctgc cctgcaaaca gaccaaagag 104520
actaattttc gtatcaacca gagatctctg caatagggaa aagaacactg tatttcctgg 104580
tcaatccacc aggatcccag gtgcctcctc ctggcatatc tctggctgat atgcaaatta 104640
gtctctttgg tcagtgtgca gtgccctagc tggtgtgcag gatggccagt tgagaccctg 104700
gccagtgtct tgacaagcag aactggtcac cctcccctgc atgtagaggc cacataaatg 104760
ccccacactc aggtgtgcct ccaaatgcac agtggatgcc cctcagaccc agccacgaga 104820
gctgtcctcc agagctgtct gtctggagct ctgggaaaca ggcagggcca gaaggacacc 104880
caggaagcca gtgaacattt cctggagagt ccagcaagag gaggaggtat ctgggatgct 104940
ggtggattga gcaggaaatg cagtgttctt ctctatccca ggctcaccct ccgggtcctc 105000
ccacaccgaa gaatctttgt caagtgtgga gaactgtgat ccttcctgat tcataacatt 105060
ctgtgcttcc tgttgccccg attgagtcca ggcccccagg cctggttccc gcagccccca 105120
tggcagctct gcctgccttt cccgcctcac cagcctatcc tcaagtgatg gccccattgg 105180
tcacagagga gtcctacctc tgcccagggt ctaaccctcc tccaatccac tccacacctg 105240
catcatctcc accacggcca ccaggtcagc cagtgagatt tggttcccgg tgatgaacat 105300
cttatcctgc agaaaatact cctcaaagag ctgcaggctg ttcttcacct cttccactgc 105360
atgctccatc ttctcagctg aaacttcctt ccctgttatc tttgggatca gcaactggcc 105420
agggttggga agaggaggga agaggaggct gtactccagg gccacctgcc ctgccaggtc 105480
tctgtactct tgtctgctgg atagatattg aacacttccc aggatataaa gcagtttcac 105540
ctcttttagc agttctgatt ggtggaaggt gccgggaacc atgcgttcac aaggatttgg 105600
ggagctcagc aggcataagt cctgtgattg attagtgatg ccggtcacag gcatggaatt 105660
tgaagtagag acacgtgtac tggttatttg tcatcttcca attttctttt ttcttctttt 105720
tttttttttt tttttttttt tgagacggag tctcactctg ttgcccatgc tggagtgcag 105780
tggcgggatc ttacctcact gcaacctccg cctgctgggt tcaagaaatt ctcctgcctc 105840
agcctttgga gtagcgtgga ttacaggcac atgccagcat gcctgggtaa cttttatatt 105900
tttagtagag atggggtttc accctgttgg ccaggctgtt attgaagtcc tgaccttgtg 105960
atccgcccac ctcggcctcc caaagtgctg ggattacagg catgagccac tgtgcccggc 106020
catcttcaaa ttttctatga gccatttttt tttttttttt tgagatggaa tctcactttg 106080
tcacccaggc tggagtgcag tggcatgatc tcggctcact gcaacatcca cttccctggt 106140
ttaagcgatt ctcctgcctc agcctcctga gcagctggga ttacaggtgc ccaccaccac 106200
accagctaat tttttttgta tttttagtac agacagtgtt tcacaatgtt ggacaggtag 106260
gtcttgaact cctgacctca agtgatccac ctgcctcggc ctcccaaagt attgggatta 106320
caggcgtgag cactgctcct ggccttctgt gggccttttg acgctgagat tctctagttc 106380
agaatgaaat gcctcgaatg ctgtcctggg tgagtcacat cagcccctcc cgtatgccct 106440
tcccctcgcc ctaataagac tctttcatgc ccattgtttc agtccacact cctgacggct 106500
ctgtaaggcg ggcaggagaa ggagctgagg aaatgaggcc cagagaggga gggagacttg 106560
cttgaggtcg ccggcagtca gcagggccag aactggcctc cagccttcct gacttccctg 106620
tgcccgtgtc cccaagcccc agaagtggcc ctgctcacct tgagccagac tatcttcttc 106680
atgggcagct gaaaggccgt gtgttgccaa gccacgaact catccacacg ggcacgtgcg 106740
tgcgggtctg gcgggcacca gtgcgatggt gcgctgtact tgcggcacag gtagtaaagg 106800
atggccgcgc tgcagaaggg gccggtcagg ggcactgccc ttgccttcct gagtgccact 106860
acatcaacca ccccggtgtg gcctgggccc aactgctggg gcttccagag caaagaggag 106920
cccaaacggc cccgagaaag accttcacca gagctgtctg tctgacagtc agtaagggct 106980
gggaaggagc cctgcggggt gagtaggagt tgggggctgg tggtataaca aagagtaggc 107040
cagcaggggg aacaacacgt gttgaattgg gatgctgagg tgggaggatc acttgatccc 107100
aggaatttgg ggctactgtg agccaagatc acaccactgc actccagctt gggtgaaaga 107160
tcaagatcct ttttcaaaaa caaaaacggg ggggcacgat ggctcacacc tgtaatcccg 107220
gcactttggg aggccaatgg gggcagatcc cttgaggcca ggagttggag accagcctgg 107280
ccaacatggt gaaaccctgt ctctactaaa atgaaaatac aaaaattagc tagttgtggt 107340
ggcacacacc tgtaatccca gctacttggg aagctgaggc acgggagtca cttgaacctg 107400
ggaggcagag gttgtagtga gccaagattg tgccactgta ctccagcctg ggccacagag 107460
caagactctg tctcaaaaaa ccaacaaaga aaaacacatg ctgaaatacg agggtaaagg 107520
gagcaaggta aatctgaaga aaagagagta gggggttgca actggaagaa gggtgggggt 107580
gattggggag tgatgaggca gccagagaca ctgtggagtc cacggagggt agcccctgga 107640
ggtgcaggga ggttatggac ttaatgctta agattaggca ttatataagc cagggcatga 107700
aaggatccat ctctctggtg ctggatggag ggtgagcccg agggggcaga atggacaatg 107760
agggggccag caactatcgg gaaggttgtg gtgtctggga atgttggagg ccatggggac 107820
agagggaagg ggatggaggg gagacatgct tcggagggga tgtcctaggc cttgctgatt 107880
gatggctggt gtgggaacct ccgcagcaca agggctcctt tatcatcacc agcagcaacc 107940
atgccaaggt aaaaaggtca gggcatggag agagctatcg gttaaaaagt ggcaggagag 108000
acagcaactg gctgcaagac tcagaacttc ttggctgggc acggtggctc acgcctgtaa 108060
tcccagcact ctgggaggcc gaggcggggg gatcatgggg tcaggagatc gagaccatcc 108120
tggttaacac agtgaaaccc cgtctctact aaaaatacaa aaaaattagc caggcatggt 108180
ggcgggcacc tgtagtccca gctactcagg aggctgaggc aggagaatgg cgtgaacccg 108240
ggaggcggag cttgcagtga gccaagatag cgccactgca ctccagcctg ggcaacagag 108300
cgagactccg tctcaaaaaa aaaaaaaaaa aaaaacttct ttggatcctg atccaaacaa 108360
actgccaaga aaatgtttag gagataatca tagagttttg aacaggagcc acatattaga 108420
tgaaatccag gaattattgt taattttatg aggtatctta atggtatcgt agtgatgcta 108480
cgctctatcc tagcccaggc tggagtgcag tggcgcaatc agagttcact gcagttctga 108540
acttcctggc ctcaagcgat cctcccgtgt cagcctctgg aagtgctcgg attataggca 108600
tgagccacca cacccagcct gttgcttttt ttttgtttgt tttaagaact cttatctctg 108660
aaaagtatgt tcctaaacat ttattgattt atttacttat ttatttttat ttttgagatg 108720
ggatctcact ctgttgccca cgctgaagtg caacgacgca gtcttggctc actgcatcct 108780
ctgcctcctg gctcaagcag tctttccgcc tcagcctccc gagtagctgg gactacaggt 108840
gcagaccacc atgctggcta atttttgtat tttttgtaga gatggggttt tgccatgttg 108900
tctaggctag gctggtcttg aacacgtgag ctcaggccat cccctcactt cagcctctca 108960
aagtgctaga attacaggca tgagctggct tctaaacatt tatgaatgga atgatggggt 109020
gtctgggagg caggggaata gaaatgatgt aaactggacc ccaagttggc aagagtcaga 109080
gctgggcgat ggatttgtgg ggttcctcgt gtccctcatt agttagtatt cactctcctt 109140
tagtgcacgt gtgagatttt ccatggtcaa acagacaaat gcttgcactg aacctcccag 109200
gagaagcaga gacagatggt gcaagggccc cagggaagac ttacctttca cttaagataa 109260
atttcccatc tttgaggctg ggcagcttcc tgagggggtt gatgtcaatg tatcctttgc 109320
tgtggtggtg acctgggagg ggcagggaag gtctgaggct gtgggactcc aggggagaga 109380
gaactgagac tcccagagac ccaaacgcct ccctctctat tttctcaaga agagggaact 109440
gaggcccgga gggacattgc gtctcacccc aggtcacagg gcaaggcagt tgcagaaccg 109500
gactgcgatc agaactgctg gctcccagcc tgctccaccc taggtttggt gactcccgtg 109560
cctcctacct gtgtcccagg accaggacga cccttttacc cagaagccgg aggcctccag 109620
tgcccacccc caaagctgga tctgaaaaca cagcctttga atcacctgaa gccctgaggg 109680
cctgggtccc atccgcaatc ccatcgctct cactctgtct ccactttaag gaagccaggc 109740
ccagcacaca gctggacatc caaagggaag cttctcggac acaatcaggg tcatcttaac 109800
agggaacctg aggtgggggc aggaactgaa actcttcctg gaccagccgc ctccagttgg 109860
aaacatttct gggggctcca ctcgcagccc gttcatttcc acagcttccc tgtctcttcc 109920
tctgtgttct agaggcttct gcttttgcag gctgagcttt tggagtccct ctgtgctggg 109980
gatggagttg gagcccaccc ctctgaccct cactcagggt tagtggagcc ctgagccttt 110040
ctgaacactg gggaggatgg gtgtagacgg actgtgcact tctgccccct ttgccaacct 110100
ggtgggcagg tgctgagttc acaaggtcct agaatcccac aaggaagcca gggtgcctgg 110160
tgggagccca gggagtccca gctactgttc cttccccctt ctcctcgaaa agcctgttca 110220
tctgtggcgt ggggactgtc attagtgagc actgactaag gtaggctgga caaggatgca 110280
gcctacaagc cgcgtggcat cttttccttc cctgtggacc tctggggtga ttcccttgtc 110340
tctgtctctg ctcctcagaa acgcccctat caggctgtgc gcggtggctc acgcctgtaa 110400
tcccagcact ctggaggctg aggtgggcag atcacttgag gtcaggagtt tgagaccagc 110460
ctggccaaca tggtgaaacc cctgttaaaa atacaaaaaa ttagctgggc gtggtggcat 110520
gcacctctaa tcccagctac tcgggaggct gaggcaggag acgcacttga acccagcaga 110580
ggttgcagtg agccgagata gcaccaccgt actccatgct gggcaacaga gcgagactcc 110640
atcaaaaaac aagaaaaaaa gaaaagccgc aatctgtgtg tcctgcctcc ccccaggacc 110700
aggcctgcca ggcagcagtg ggagttgacc tttcagcaga tccacaaact gaaagttgaa 110760
ctggatgtca tgcttcttcg agaagatgta gacggcacgg cagggtgctg acagcaggtc 110820
catgtagagc tccagtgcca tgttgagaca catgccaggc cccacagccg cagttggcca 110880
gccacagacc tgggcctatg tctggccaga gtccctggcc ctgtgccctc tccgatctgg 110940
gcccaggatc ctgtgttccc cagggaaacc tcttgtttcc ctttgtgttg tcataaggcc 111000
aggaagcctg caattctcac agcatcaagg attctaagga ggcccaggag taggctgggg 111060
agaggcccgt ggcaaaggtg tggcagccgt gaccctactc tcccccttcc acgtgtgcct 111120
gtgccccgtg gtgccacctc acagacacca gtctgagaag ggattatgcc tgggaattcc 111180
cacggctgga ttttcattgc agaacctgac gaaaggggct ttgcagggtc cagaatgaag 111240
aggaggcaat gagaattatc cctggaggat tctagaagta gaggctggga gtatccacag 111300
gtaaatcgag cctgaactat gactagaaag gaattgggag aaagagacac aggtgaatcg 111360
agcctgaact atgactagaa aggaactggg agaaagagac acaggaaact gtgagctttg 111420
ggagcaatgg ggacaccacc accaggaagt cagggggcac tcagccggtg tgtgccacac 111480
agaggagcct agaaacttcc tggccttggt tggggctgca gtggccagac tgtgtacctg 111540
gtggccaagg aaggtaacta gagccccacg tagaggactg agtgccactc actctatgct 111600
gtgatctaat aggtctaggc tgagaaatgg gactgacccc acttctggtg acagagtaag 111660
cctggagaca agcgaagagc atgcagtgtg tttattgcag acagcagggt gcagtggagt 111720
gggctgcacc cactgcacct gcttctgggc cagctggggg tcccgccaag cctcactgtg 111780
gttcagaggc tggagtatga gcttgtgggc ctccagcaag agctcagtac caagggcagc 111840
cgccacacgg gcctgccaca ctgccagccg gggcagtctt ggaagaggtc gcagccaacg 111900
gcagtgggct gggaagacag ggcagctggg gttggggctc agccccatgc ccagagtccc 111960
cccagagggc aggtgggtag gaaagcttca cctgcatcac ctccgtcagc accaagtctt 112020
ccagggagat ctgctccatt gccaggaagg gcctggccac cagcaccccc caatccaggt 112080
gctgcagggc tggcatcagc ctccccaaca gccgctccag ctgtgcagca tctgcaggct 112140
gtccggagaa gtggggcagg agagactggg ctgggaggag aggagcaact taacccagac 112200
tcttggaagc ctcttgggcc tcctctccat cccttcccgg ctgccactca ttagtgatgt 112260
cctctggggc tctgtgttca gaaggtttct aaggattcat tcaggcttag gggaaggagt 112320
actgtgatag attagttatg tctgctgagg acagtggaag tgaagctaat atgtaatatg 112380
taccaactgg tgccttccat ctctgggggt ccaggcccct gccccagcct gcatgacagg 112440
gcctaggaag ctcaccttgc acaggtagac attggtggca ggcagctgga tggtgacatg 112500
cttccacgcc aagtactcat ccacgcaggt gcgggcttgc agctcaggtg ggtaccagtg 112560
tccccgtatc tggtactttc gactcaggta taaaacgatg accatgctgt gggcaggggg 112620
tgtcagagat cttttctcca gcctgtcagg gccaggtggc ttcattgctc atactcaggg 112680
tgatttgacc aggttttctc cccaccttca ccttcagggg aggacctccc ctatactgca 112740
actccctcca ggcagtatct ccccacgacc aaaccactct gtttctccat ttccaactct 112800
gatcctccct gggcccatgt ttccacctcc cctcccagtt accataccat cctgccctct 112860
gtgcccaggc cccactccct ggcatcaggg tccccactca gagtgcctgc cgtgtgggcc 112920
ctgcccaaaa ggtctccaag ttcccttcct agtgccccag aacactaggt gcccattttc 112980
tatcctcagg taagccctgg gaggtggata cattattcct cccactccgt tttacagagg 113040
agaaacctgg cacacaaagt tctgtgattt ccccaagggc atggattgag gaagtggtga 113100
gctgggattg aatgtggagt ctcccctggg tctgcccagc tcctaggact ctgaaaggat 113160
gagctgggga catttgggga ggagccagcc tcaagcctgg gccttgcccg ctggcttcag 113220
gttgtccagg tttatccggg tgacctacgt cctttcccca cctgggcagc tccagctggc 113280
tcagactcta gccaggttcc agcctccagc tttagcccaa aggtgcccct gggtccagcc 113340
tctgcatcgg agctacacag gctgaaatga ggaaggggcc tcagtgattt ggaaagatct 113400
ccaagccaag gaaagatctc ccctctcgcc tctgcctccc cctacaactc tgagccccag 113460
aacccaggca gatcctggct ctgaccctgg gtgggcacca ggtcaccatt acctctttgc 113520
tagtaggaag tcgccatctc tgagggcagg caccttcccc agggggttca ccttcaggaa 113580
ctcaggcttc aggtgctccc ttggtgggca gaaaggcgag aaggctcagt gtagggcctg 113640
gtccttggca cccactccat ggggcccagc ccagccacct ggctcacccc aagccaactc 113700
caccatccac agctcaaaag ggatgtcatt ctgtcttgca aaagtggata gtgtgtcagg 113760
tccaggaaga gctcaagccc cattgctgct cctctcacct gccctcccca gaattctgga 113820
cctccaggag ttgctcagcc cttctccact agttctgagc tgtttcctgt ccatagtctg 113880
ctgtagtggg ccagagcaca gggcagattt gtcttcaggg actttctctg ggcattcctg 113940
ggcagagaac ttcatcttct ctgttaactc ttgctgataa ttctctgtag ctaggcacct 114000
tgtaaaaccc atgtcccaga atgccaaacc tggaaatgca atgtcagcgc cgaaactgca 114060
ttgaaactga atgaatgagg tccccaccct cttatctaaa tatcctatca gtctgttgaa 114120
tgtcaaagta tgtaggggtg gtgtggggga gcctataggg agtctccatt tctgtagctt 114180
tccacatgtt ccatgcagaa ttttcctcta atgacccttg gacaccgtgg tggtcactgt 114240
ttccctgcat aaacctttga tgatccttat ctttagcgta ccagtgaaac accaaattca 114300
gattttcagt ctgtgcagtc tatgtagtta ataaaatcaa tttctaatgg aatatgcctg 114360
tgcctggtgt gtttgtacct aaccactgct aaggactttc attgatagct agaccttgga 114420
gttccttccc ctggacccct gacccaagta ttttgcactc attcctctgc cagtgtgttt 114480
catggtgtcc tgcttcccag agtgcctcag ggaatgcttg cattggatat aaatgaatcg 114540
ttgcggtcac catggatggg attcaggcct aactctcacc aagaccataa ggccacagtc 114600
tttgatatag attccccatg ttaaatctgc catcctcagt gtagtgagag tgatattgag 114660
agagaaaagt taggtaatta aggtgggtcc ttggtaaaac tcctttaaac agaggaacag 114720
cctgaaaaat caggcttcag gcacagataa ggaaacttgc acaaacctct ggctcactca 114780
gataaaggaa caatgcccaa cacagaaaca cctttgatct ttgtgcctaa ggcatgccca 114840
cagctacttt gataagggaa caagacccag catagaaatg cctttgtcct ttgtataacc 114900
agtgggcttc caggaaatag tctcttctcc ttttgtggac atgtacacgg tgggctacag 114960
tgggttccag tgggcacttt cctttcttgg acgtgcttta gaatgtgagc ctctgattgg 115020
tcctgggcca ggctatcact tcagctcctg attggtccct caaggtccag gggccaagct 115080
gagtagtgct ttctccaaga ccagtcagta cattccttcc tttcccagtc cataaaaccc 115140
cctgacccag cctcataatg ggcaacctgc ttgggccccc cgccagctgc aatgagcttt 115200
cttcatttgc ttattaaact tttgcttcaa ccttaccctt tgtgtccaca ctccctaatt 115260
ttcttggtcg tgagacaaag aattctgggt gataccccac aatgagagac tggtacattg 115320
tggtgcacgg gcaagactgc aacaatatgt tttctgcttg cttgtttgga ctggcctctt 115380
cctaactctc cagagtgctc tctccccact gtggccctga gctttgcctc acggtaacac 115440
tgtgctgcat gacactccag catgatctgc atgtttctca cctccggacc ttccccacac 115500
aactttcttg ctgcttggac cacccttccc acctattttc aactgtttct ctccagcgca 115560
tctttcatga taaggcttga gtggaagctg tatccgaggc tgaaggttat ccatgcagct 115620
ttgaaaccat ctgtttatgc ttctgtttac cccccaaata ctgtttaccc caatactcaa 115680
agtcaaggtt ggtgactcaa caattttcag aacttctagc ttatcagtat agctttattg 115740
tactgaactg agtttttcac catgctgtta ctgtctttga ggttaaaaag cccccaacat 115800
tcactcctat ttgaatcatc tgtgcaagac tcaagcagct atgaattcaa ccacagtgta 115860
aagttcttcc ctacaatgct ttattgttat tctagctccc aagagagtac catctttcag 115920
cccaagaccc ttgacagaac cccttcaact ctgtcgcctt ttttcatctc tggaattctg 115980
acaccaccca tgacccctct tcttgttcat ttactaggtt ttgttccttt ttttcctttt 116040
gtttcaatat cctattgtat ctgttccttt tgggcttcac atcctttttg gaaagagagg 116100
cacaggtaat ataatgaatg aggatgctcc tcctccctgg ttccctctaa gtcaagtttc 116160
tccaacgcgc tggtctgctt ggctttgaat gcggctcaac acaaattcat aaactttctt 116220
acaacatttt gagatttttt ggtgattttt atttttatta tttatttatt tattaactta 116280
tcagctatcc ttagtgttag tgtattttat gtgtggccca aaacaattct tccaacgtgg 116340
cccagggaaa ccaaaagatt ggacaccccg ctctaagtgt tccccaagtt tccactgttt 116400
tcagagcaca tgtcccaggt aataacacac agcccatcat ttagtgactg ctcagggtgc 116460
aagctgtgga gaagagagtc cgactacgag ctggtgctct aagcctccag cacgctagga 116520
ctgacgacgg ggatccggaa acaacaagct ccactcccgc aacacacaca cacacacaca 116580
cacacacaca cacacacaca cacacgcaca cgcacacgca cacgcgcacg cgcgcacaca 116640
cgcgcacaca cgcgcgcacg cgcgcgcgcg ccgcccaagg gtagccgtct gcagggcccc 116700
agctaagcag cccgcggaga gcgagcggcg cgtgcgcccc cgctgattgg ctggcacaac 116760
cggcaccgga gccaatggga gcacgccttg ttgtcaggcg cgcagccgga cggcgcggtg 116820
gtcgggacag actggccgtt gctgtggaga cgcctggtga gttcctggaa ggctggtttg 116880
aaggcggtgc cggggtgggg gtgaggctca gcagagcccg gggcacaggc cggaggggct 116940
gcaggcgcga ggctgggtta cgtgaggaag ctgggggttt cgcgggcagc tttagagccc 117000
cagtcaggga aaccgaggcc gggcttcctg gctgcctcgc gagcctcttc atggctctcg 117060
ccgccgccct gaggtgccta gaatgggttc cggcctccgg ggaggttccc agtaaccgca 117120
ggagccacca ttgatttggc gtctgctggg tgcaaagccc agcgcgctaa ccctttactc 117180
gcgacctttc gcttcacctt cacagcagcc ctgcgaggtg agtgttgtta ttggtgccat 117240
ttacaggggt ggaaactgag gccgcgggta ggtgaaatga tttgctccaa gtcacccagt 117300
gggtgagcgc gactccagac tgaaaatttt gaccgttctg cttcactgcc tccccgcaag 117360
aggcgggccc tggccagatg gaactcacct ggcattccca aggtatctgc acaagtggct 117420
ttgggagtca cttaactctt caggacctgg gttattacag gttataagtg tgggaccgtc 117480
ggattccatg tggctaagat tctttctatg tgattattgt ggttagaaat agaatatgct 117540
tagaacagtc tcgttcagcg gtcggcaaac ttttgttgta aatatatcag tgtttacatt 117600
gttatattgt aagtataatg agtataatag tattcataat agtagtttag gcttgggggg 117660
tccatactct gtctacttaa ctgtagcgat gtagtgtgga tacagccaaa gacaatacat 117720
aacagactag ctgcgtcgca ataaaatttt aattataggg accgaagttt taattttata 117780
tattgttaat ataattttca cgtgtcatga ttttttttcc caaatatgaa aaaatgtaaa 117840
aaccattctt tgctcgtggg ctgtacaaaa agggtgggct ggttagcttg cagaaccctc 117900
atctagtaca aagcctcata catgagggga aacagactga gagactgaca tctatgtcaa 117960
gtcagtcaca cagccagggc actgccacca ctgagcttaa gacataggct tcccaccttc 118020
caggccagga aacatcccca gttatcactc tggatcaggg tgctgaggtc tgaaggtgtg 118080
ctactcttta tcgtgttctg aggccatgga aactatattc taaggggttt ggaaacttat 118140
tagcttagac taataagcct ggtgaatgtg ctgctatctg aggaaatgca gctgctgggc 118200
tgggagtcag aaatgaatag atatttttct ttttcatgat tattcaatag cttttttgtg 118260
tgtgtgtgtg acggagcctt ggtctgtcgc ccaggctgga gtgcagtggc atgatctcgg 118320
ctcactgcaa tctccgcctc ccgggttcaa gcagttctcc tgtctcggcc tcccgagtag 118380
ctgggactac agacacccgc caccatgctt ggctaatttt tgtattttta gtagagacgg 118440
ggtttcaccg tattggtcag gctgctctca aactcctgac ctcaggtgat ccacccgcct 118500
cggcctctga aagtgttggg attacaggcg tcagacaccg cgcccggcct caatagcttt 118560
ttttttttta aaagctcctt ttagtgagtt tcttggattt atggtgaaca atacataaat 118620
ggttaccatc ctcacattgc ttacagatga taaatatata atcaaaatgg ttatttcttt 118680
ttaagctttt tttttttttt tttttgagat ggagtctcgc ctgtggccca ggctggagtg 118740
cagtggtgcg atctcgcctc actgcaagct ccgccgcctg ggttcacgcc attctcctgc 118800
ctcagcctcc ctagtagctg ggactacagg cgcctctcac cataccctgc tagttttttg 118860
tattttttag tagagacgga gtttcaccgt gttagccagg atggtcttga tctcctgacc 118920
tcatgatccg cccgcctcgg cctccaaaag tgctgggatt ataggcgtga gccaccatgc 118980
ccggcctctt tttaagctt 118999

33

7

DNA

Homo sapiens

misc_feature

Sequence of Figure 4 HA5

33
acgtcca 7

34

7

DNA

Homo sapiens

misc_feature

Sequence of Figure 4 H0 and HA3

34
acgccca 7

35

7

DNA

Homo sapiens

misc_feature

Sequence of Figure 4 3?sequence HA5

35
atgcagt 7

36

7

DNA

Homo sapiens

misc_feature

Sequence of Figure 4 H0 and HA3

36
atgtagt 7

Number	Name	Date	Kind
5552283	Diamandis et al.	Sep 1996	A
6025480	Massague et al.	Feb 2000	A
6150514	Swensen	Nov 2000	A

Method for determining the presence of at least one allele of a GSTT1 deletion mutant

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Inventors

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Agents

CPC

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US

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Abstract

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Claims

Priority Claims (1)

US Referenced Citations (3)

Non-Patent Literature Citations (14)

Entry
Walker and Little, from DNA PROBES, 2nd ed., section 7: Amplification Systems, pp 255-269. MacMillan Publishers LTD, 1993.*
Research Genetics, “Designer PCR”. from Nucleic Acids Research, vol. 22, 1994.*
Genbank Accession No. AP000351, 2nd version, Jul. 8, 1999.*
Keinlinen et al: Clinical Chemistry, vol. 36, pp. 900-903, 1990.*
Carter, D Human DNA sequence from clone 322B1 on chromosome 22q11-12, complete sequence. Accession No. Z84718, NCBI, Nov. 23, 1999.*
Van Sloon P P H et al:“Determination of spontaneous loss of heterozygosity mutations in Aprt heterozygous mice” Nucleic Acids Research, vol. 26, No. 21, 1998, pp. 4888-4894.
Fortina P et al:“Flourescence-based, Multiplex Allele-Specific PCR MASPCR) Detection of the DeltaF508 Deletion in the Cystic Fibrosis Transmembrane Conductance Regular (CFTR) Gene” Molecular and Cellular Probes, GB, Academic Press, London, vol. 6, Aug. 1, 1992, pp. 353-356.
Lohmann D et al: “Detection of Small RB1 Gene Deletions in Retinoblastoma By Multiplex PCT and High-Resolution Gel Electrophoresis” Human Genetics, DE, Berlin, vol. 89, No. 1, 1992, pp. 49-53.
Shi M M et al: “High-throughput genotyping method for glutathione S-transferase T1 and M1 gene deletions using TaqMan probes” Research Communications In Molecular Pathology And Pharmacology, vol. 103, No. 1, 1999, pp. 3-15.
Kelsey K T et al: “The glutathione S-transferase theta and mu deletion polymorphisms in asbestosis” American Journal of Industrial Medicine, vol. 31, 1997, pp. 274-279.
Oke B et al: “GSITI null genotype frequency in a Turkish population” Archives in Toxicology, vol. 72, 1998, pp. 454-455.
Abdel-Rahman S Z et al:“A multiplex PCR procedure for polymorphic analysis of GSTMI and GSTT1 genes in population studies” Cancer Letters, vol. 107, 1996, pp. 229-233.
Weincke J K et al: “Gene deletion of glutathione S-transferase theta: Correlation with induced genetic damage and potential role in endogenous mutagenesis” Cancer Epidemiology, Biomarkers & Prevention, vol. 4, 1995, pp. 253-259.
Pemble S et al: “Human glutathione S-transferase Thets (GSTT1): cDNA cloning and the characterization of a genetic polymorphism” Biochemical Journal, vol. 300, 1994, pp. 271-276.