Claims
- 1. A method for identifying substances that affect the interaction of a presenilin-1-interacting protein with a mammalian presenilin-1 protein, or a fragment, variant or mutein of said mammalian presenilin-1 protein that binds to said presenilin-1-interacting protein, comprising:
- (a) providing a preparation containing: a mammalian presenilin-1 protein, or a fragment, variant or mutein of said mammalian presenilin-1 protein that binds to said presenilin-1-interacting protein; a presenilin-1-interacting protein; and a candidate substance; and
- (b) detecting whether said candidate substance affects said interaction of said presenilin-1-interacting protein with said mammalian presenilin-1 protein, or a fragment, variant or mutein of said mammalian presenilin-1 protein that binds to said presenilin-1-interacting protein.
- 2. The method of claim 1, wherein said mammalian presenilin-1 protein is defined by an amino acid sequence set forth in SEQ ID NO:2 or SEQ ID NO:4.
- 3. The method of claim 1, wherein said preparation contains a mammalian presenilin-1 mutein.
- 4. The method of claim 3, wherein said preparation contains a mammalian presenilin-1 mutein of the protein defined in SEQ ID NO:2, and which has at least one amino acid substitution selected from the group consisting of I143T, M146L, L171P, F177S, A260V, C263R, P264L, P267S, E280A, E280G, A285V, L286V, L322V, L392V, C410Y and I439V.
- 5. The method of claim 1, wherein said presenilin-1-interacting protein is an S5a subunit of 26S proteasome.
- 6. The method of claim 1, wherein said presenilin-1-interacting protein is a GT24 protein.
- 7. The method of claim 1, wherein said presenilin-1-interacting protein encoded by a nucleotide sequence selected from the group consisting of SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:40 and SEQ ID NO:41.
CROSS REFERENCE TO RELATED APPLICATIONS
This application is a Continuation-in-Part of U.S. application Ser. No. 08/592,541, filed Jan. 26, 1996, and claims benefit of U.S. Provisional Application Ser. No. 60/021,673, filed Jul. 5, 1996, U.S. Provisional Application Ser. No. 60/021,700, filed Jul. 12, 1996, U.S. Provisional Application Ser. No. 60/029,895, filed Nov. 8, 1996, and U.S. Provisional Application Ser. No. 60/034,590, filed Jan. 2, 1997, and all of which are incorporated by reference herein.
US Referenced Citations (4)
Foreign Referenced Citations (13)
Number |
Date |
Country |
2054302 |
Apr 1992 |
CAX |
2071105 |
Dec 1992 |
CAX |
2096911 |
Nov 1993 |
CAX |
WO 9119810 |
Dec 1991 |
WOX |
WO 9400569 |
Jan 1994 |
WOX |
9410569 |
May 1994 |
WOX |
9423049 |
Oct 1994 |
WOX |
WO 9634099 |
Oct 1996 |
WOX |
WO 9703086 |
Jan 1997 |
WOX |
WO 9703192 |
Jan 1997 |
WOX |
WO 9703999 |
Feb 1997 |
WOX |
WO 9708319 |
Mar 1997 |
WOX |
WO 9746678 |
Dec 1997 |
WOX |
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
592541 |
Jan 1996 |
|