Claims
- 1. A method for producing a stable polymerized hemoglobin blood-substitute from a hemoglobin solution, comprising the steps of:
- a) contacting the hemoglobin solution with a dialdehyde, thereby forming a polymerization reaction mixture;
- b) polymerizing the polymerization reaction mixture, thereby forming a polymerized hemoglobin solution;
- c) contacting the polymerized hemoglobin solution with an alkaline solution to form a basified polymerized hemoglobin solution having a pH greater than about 10;
- d) reacting the basified polymerized hemoglobin solution with sodium borohydride, thereby forming a stable polymerized hemoglobin solution; and
- e) filtering said stable polymerized hemoglobin solution to separate tetrameric hemoglobin from the stable polymerized hemoglobin solution, thereby forming a stable polymerized hemoglobin blood-substitute.
- 2. The method of claim 1, wherein the dialdehyde comprises glutaraldehyde.
- 3. The method of claim 1, wherein the hemoglobin solution comprises bovine hemoglobin.
- 4. The method of claim 1, wherein the hemoglobin solution comprises hemoglobin selected from the group consisting of: human hemoglobin, primate hemoglobin, ovine hemoglobin, and porcine hemoglobin.
- 5. The method of claim 4, wherein the hemoglobin is transgenically produced.
- 6. The method of claim 4, wherein the hemoglobin is recombinantly produced.
RELATED APPLICATIONS
This application is a Continuation-in-Part of copending U.S. patent application Ser. No. 08/458,916, filed Jun. 2, 1995, which is a Continuation of U.S. patent application Ser. No. 08/409,337, filed Mar. 23, 1995. This application is also a Continuation-in-Part of U.S. patent application Ser. No. 08/209,949, filed Mar. 11, 1994, now U.S. Pat. No. 5,618,919, which is a Continuation of U.S. patent application Ser. No. 07/820,153, filed Jan. 13, 1992, now U.S. Pat. No. 5,296,465, which is a Continuation of U.S. patent application Ser. No. 07/119,121, filed Nov. 10, 1987, now U.S. Pat. No. 5,084,558, which is a Continuation-in-Part of U.S. patent application Ser. No. 07/107,421, filed Oct. 13, 1987, now abandoned, which is a Continuation-in-Part of U.S. patent application Ser. No. 06/928,345, filed Nov. 10, 1986, now abandoned. The teachings of the patents and patent applications noted above are incorporated herein by reference in their entirety.
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Continuations (3)
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409337 |
Mar 1995 |
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820153 |
Jan 1992 |
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119121 |
Nov 1987 |
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Continuation in Parts (3)
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209949 |
Mar 1994 |
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107421 |
Oct 1987 |
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928345 |
Nov 1986 |
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