Claims
- 1. A process for producing high levels of recombinant factor VIII:c protein or its analogs which comprises the steps of:
- (a) adding a serum-free preparation of phospholipid to a serum-free culture medium containing mammalian cells transfected with an expression vector containing DNA encoding recombinant factor VIII:c protein or its analogs, said phospholipid being added in an amount greater than 100 ug/ml of culture medium,
- (b) culturing said cells in said medium under suitable culture conditions to produce said factor VIII:c or its analogs, and
- (c) recovering said factor VIII:c or its analogs from said medium.
- 2. The process according to claim 1 wherein the DNA encodes human Factor VIII:c.
- 3. The process according to claim 1 wherein the mammalian cells are CHO cells.
- 4. The process according to claim 2 wherein the mammalian cells are CHO cells.
Parent Case Info
This application is a continuation of application Ser. No. 07/260,085 (filed Oct. 19, 1988) now abandoned, which is a continuation-in-part of application Ser. No. 816,031 (filed Jan. 3, 1986) now abandoned, and application Ser. No. 942,338 (filed Dec. 16, 1986) now abandoned, and application Ser. No. 034,882 (filed Apr. 6, 1987) now abandoned, and application Ser. No. 068,865 (filed Jul. 2, 1987) now abandoned, the contents of each of which is hereby incorporated by reference.
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Related Publications (3)
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Number |
Date |
Country |
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942338 |
Dec 1996 |
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34882 |
Apr 1987 |
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68865 |
Jul 1987 |
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Continuations (1)
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Number |
Date |
Country |
Parent |
260085 |
Oct 1988 |
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Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
816031 |
Jan 1986 |
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