Claims
- 1. A method of optimizing the expression level of a monoclonal antibody which comprises:
(a) producing a eucaryotic host cell containing and capable of expressing a first DNA sequence encoding an antibody heavy chain, said first DNA sequence being associated with a first heterologous selectable amplifiable marker gene, and a second DNA sequence encoding an antibody light chain, said second DNA sequence being associated with a second heterologous selectable amplifiable marker gene, (b) culturing said host cell in a suitable culture medium. (c) measuring the relative amounts of said first and second DNA sequences expressed, and (d) differentially amplifying said amounts of said first and second DNA sequences with appropriate selective agents to allow maximized production of said antibody.
- 2. A host cell produced by the method of claim 1.
- 3. A monoclonal antibody produced by the method of claim 1.
- 4. A method of optimizing the expression level of a monoclonal antibody which comprises:
(a) producing a eucaryotic host cell containing and capable of expressing a first DNA sequence encoding an antibody heavy chain and a second DNA sequence encoding an antibody light chain, said first and second DNA sequences being associated with a first selectable amplifiable marker gene, (b) culturing said host cell in a suitable culture medium, (c) measuring the relative amounts of said first and second DNA sequences expressed, (d) transfecting into said host cell a third DNA sequence associated with a second selectable amplifiable marker gene, said third DNA sequence corresponding to the DNA sequence expressed in lower amount in step (c), and (e) differentially amplifying said amounts of the DNA sequences to allow maximized production of said antibody.
- 5. A host cell produced by the method of claim 4.
- 6. A functional monoclonal antibody produced by the method of claim 4.
Parent Case Info
[0001] This is a continuation-in-part application of pending U.S. application Ser. No. 07/386,489, filed Jul. 28, 1989.
Continuations (1)
|
Number |
Date |
Country |
Parent |
07765281 |
Sep 1991 |
US |
Child |
10244894 |
Sep 2002 |
US |
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
07386489 |
Jul 1989 |
US |
Child |
07765281 |
Sep 1991 |
US |