Patent Grant
9611514
The foregoing applications, and all documents cited therein or during their prosecution (“appln cited documents”) and all documents cited or referenced in the appln cited documents, and all documents cited or referenced herein (“herein cited documents”), and all documents cited or referenced in herein cited documents, together with any manufacturer's instructions, descriptions, product specifications, and product sheets for any products mentioned herein or in any document incorporated by reference herein, are hereby incorporated herein by reference, and may be employed in the practice of the invention. More specifically, all referenced documents are incorporated by reference to the same extent as if each individual document was specifically and individually indicated to be incorporated by reference.
The instant application contains a Sequence Listing which has been submitted electronically in ASCII format and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Nov. 18, 2015, is named 45981_01_2001_SL.txt and is 35,208 bytes in size.
Technical Field
The present invention relates to a method of selection of hDIS3 PIN domain inhibitors, yeast strains and cell lines used in such methods. It also relates to new therapeutic agents selected hDIS3 PIN domain inhibitors and uses thereof in the treatment of cancers having mutations in hDIS3 RNB domain, especially multiple myeloma. The invention also relates to composition comprising new therapeutic agent hDIS3 PIN domain inhibitor and method for inducing synthetic lethality in a cancer cell.
The invention also relates to the methods of selection and screening for hDIS3 PIN domain inhibitors, as well as to the methods of screening of cancer patients, like multiple myeloma for the susceptibility of cancer cells to synthetic lethality with hDIS3 PIN domain catalytic inhibition.
Background Art
Synthetic lethality has recently emerged as one of the most promising strategies for development of targeted cancer therapies (Porcelli et al. 2012). Such a strategy enables the targeting of cancer cells with a potentially low effect on untransformed cells. For example, breast cancer cells with mutations in the BRCA1 and BRCA2 are very sensitive to inhibition of poly [ADP-ribose] polymerase (PARP1), whereas inhibition of PARP1 has little influence on survival and proliferation of normal cells with wild-type BRCA (Bryant et al. 2005; Farmer et al. 2005). Lead compounds based on PARP1 inhibitors are in advanced clinical trials (Barber et al. 2013).
Multiple myeloma (MM) is a lethal neoplastic disease which accounts for over 1% of all tumors, 10-15% of hematologic malignances, and 20% of deaths related to cancer of the blood and bone marrow (Laubach et al. 2011). It is difficult to treat and no successful therapy has been found up to this time.
The genetic background of MM is not completely understood. In up to 90% of MM patients chromosomal abnormalities are detected.
Recent publication describing whole-genome sequencing of 38 MM patients provided a global view on the somatic mutations associated with this cancer (Chapman et al. 2011). Approximately 50% of patients had either NRAS or KRAS activating mutations placing them together with chromosomal translocations as major prerequisites for development of MM. Unexpectedly, hDIS3 gene, not previously described as proto-oncogene or tumor suppressor was frequently mutated in the MM patients. hDIS3 protein is a well described catalytic subunit of the exosome complex, which plays a crucial role in eukaryotic RNA processing and decay (see below) (Chapman et al. 2011). High frequency of hDIS3 gene mutations in MM patients has been lately confirmed in another high-throughput study (Walker et al. 2012).
Interestingly, hDIS3 gene mutations were also found in global screens dedicated to other types of cancer, like medulloblastoma (in this case it was a true catalytic mutation changing D479 to V, which disrupts coordination of the Mg2+ ion thereby abolishing hDIS3 exonucleolytic activity) and several cases of acute myeloid leukaemia (including a substitution of another aspartate in the active site: D488N) (Parsons et al. 2011; Ding et al. 2012). Additionally, hDIS3 was identified in transcriptomic analyses as one of the genes, whose expression differentiates superficial spreading melanoma from nodular melanoma (Rose et al. 2011). Furthermore, hDIS3 overexpression was earlier observed in human colorectal cancer and in a mouse model of this cancer, where elevated levels of respective mRNA and protein positively correlated with the incidence of metastasis (Lim et al. 1997; Liang et al. 2007). Very recently, functional genomics study defined hDIS3 as one of several genes essential for the growth maintenance of colorectal cancer cell lines (Camps et al. 2013).
The eukaryotic RNA decay and processing pathways are the essential components of the regulation of gene expression. RNA turnover is a crucial part of the cell homeostasis.
The exosome complex, which catalyzes 3′-5′ exonucleolytic RNA degradation, is a primary eukaryotic ribonuclease that plays a fundamental role in virtually all pathways of RNA turnover (Lebreton & Séraphin, 2008; Schmid & Jensen, 2008; Tomecki et al. 2010a). Altogether, it participates in: turnover of normal mRNAs and AU-rich elements-regulated decay of unstable mRNAs (Gherzi et al. 2004; Mukherjee et al. 2002); nuclear processing of stable RNA classes (snRNA, snoRNA, rRNA, tRNA) (Allmang et al. 1999; Gudipati et al. 2012); degradation of unstable transcripts arising from intergenic regions, whose presence was discovered in exosome mutants (Wyers et al. 2005); complete degradation of mRNA after endonucleolytic cleavage initiated by siRNA in RNA interference pathway (Orban and Izaurralde, 2005). Furthermore, the exosome complex plays a crucial role in quality control of RNA both in the nucleus and the cytoplasm. It participates in such cytoplasmic RNA quality control pathways as the degradation of transcripts containing premature stop codons (NMD, nonsense-mediated decay), lacking stop codon (NSD, non-stop decay), or mRNAs with some steric obstacles, which cause ribosome stalling during translation (NGD, No-Go decay) (Allmang et al. 1999; Allmang et al. 2000; Assenholt et al. 2008; Gudipati et al. 2012; Isken & Maquat, 2007; Kadaba et al. 2004; Parker, 2012; Wang et al. 2008). Most importantly, it is a primary enzyme responsible for the degradation of unwanted molecules in the nucleus, including improperly processed pre-mRNAs, rRNAs and tRNAs and precursors of their synthesis. An interesting function of the exosome is in suppressing pervasive transcription since in exosome mutants huge amount of RNAs arising from intergenic and antisense regions accumulate (Preker et al. 2008; Lubas et al. 2011). Some of such transcripts have regulatory functions and participate in chromatin silencing of the genes located near sites they arise from (Camblong et al. 2007).
hDIS3 protein is a catalytic subunit of the RNA exosome complex, which plays a crucial role in RNA processing and decay. The exosome complex has an evolutionarily conserved structure. It is composed of 9-subunit ring, which despite homology to archaeal and bacterial phosphorolytic ribonucleases is devoid of any detectable catalytic activity (Liu et al. 2006; Dziembowski et al. 2007). The catalytically-inactive central exosome channel formed by the ring subunits has important functions in the regulation of ribonucleolytic activities of the complex (Wasmuth & Lima, 2012; Drazkowska et al. 2013), which are supplied entirely by associated catalytic subunits. Ribonucleases responsible for the enzymatic activity of the exosome belong to two different families: Dis3-like proteins are similar to bacterial RNases II/R while Rrp6-like subunits are members of RNase D family (reviewed in Tomecki et al. 2010a). The exosome was discovered in yeast and the majority of the research work has been done using this model system (Mitchell et al. 1997). In yeast genome there are single genes coding for Dis3 and Rrp6 proteins. Dis3 is the only essential catalytic subunit being present both in the nucleus and the cytoplasm. This multidomain protein is endowed with two different catalytic activities: 3′-5′ exonucleolytic one residing in RNB domain and endonucleolytic one arising from the N-terminal PIN domain (Dziembowski et al. 2007; Lebreton et al; 2008, Schaeffer et al. 2009; Schneider et al; 2009). Dis3 exonuclease active site is located near the bottom of the central channel of the 9-subunit ring through which substrates are delivered (Bonneau et al. 2009; Malet et al. 2010). Both activities cooperate with each other but the exo one is more important for the cell physiology (Lebreton et al. 2008; Schaeffer et al. 2009; Schneider et al. 2009). Rrp6 is restricted to the nucleus and is responsible for only a subset of nuclear exosome functions. The exosome does not function on its own and needs appropriate cofactors for its full activity and efficient recognition of multiple substrates. The central components of all cofactors are RNA helicases, which are believed to transfer the substrate into the central channel of the ring, which then delivers RNA to Dis3 exonucleolytic active site.
There are important differences between human and yeast exosomes despite the well conserved structure, especially in the properties of the catalytic subunits. Human genome encodes two proteins—hDIS3 and hDIS3L, which contain all Dis3 elements including newly discovered CR3 motif and a structurally intact PIN domain required for the interaction with the 9-subunit ring (Tomecki et al. 2010b; Staals et al. 2010; Schaeffer et al. 2012). Proteomic analyses confirmed that both hDIS3 and hDIS3L interact with the human exosome ring, most probably in mutually exclusive manner. Notably, both human Dis3 homologues are processive 3′-5′ hydrolytic exonucleases, whereas only hDIS3 has also retained endonuclease activity in its PIN domain. In vivo localization studies and analyzes of substrate specificities revealed that hDIS3L is restricted to cytoplasmic exosome, whereas hDIS3 is mainly a nucleoplasmic protein with a small fraction present in the cytoplasm (Tomecki et al. 2010b, Staals et al. 2010). Additionally, human RRP6 is mainly nuclear and significantly enriched in the nucleoli, with a minor fraction in the cytoplasm. Thus, human RNA exosomes, although basing on the same structural scaffold as their S. cerevisiae counterparts, exist as functionally and compositionally distinct variants in different areas of the nucleus and in the cytoplasm (Lykke-Andersen et al. 2011).
Because of the unsatisfactory performance, the occurrence of severe side effects and the fact that resistance to currently used therapeutic agents is quickly developing in cancer (tumor) cells like myeloma cells, new, dedicated therapeutics that specifically affect cancer cells are greatly needed.
The information presented above suggests the existence of a molecular link between functions of the exosome complex and development of different cancers.
The present invention is based on the unexpected finding that inhibition of endonucleolytic DIS3 PIN domain activity in the DIS3 bearing mutation corresponding to mutation found in cancer patients in DIS3 RNB domain gives the effect of synthetic lethality in the cells having such mutation.
As described above, changes in hDIS3 activity, often due to mutations in hDIS3 gene, are frequently found in cancer patients, such as melanoma, colorectal cancer, medulloblastoma, acute myeloid leukaemia, and, in particular, multiple myeloma (MM) patients. Because of the importance of RNA metabolism for cell homeostasis, cancer cells harboring such mutations may be potentially more vulnerable to further impairments to the exosome complex activity. However, in the art there are no successful therapy methods or effective therapeutic agents capable of exploiting this. There are also no methods for selecting, screening and efficient testing of potential new therapeutic agents for such treatment, especially there is no method available for effective selecting and screening for new therapeutic agents, and there is no therapeutic agent which can be used in the treatment of cancers based on synthetic lethality between the mutations present in the RNB domain and the inhibition of endonucleolytic activity of the hDIS3 PIN domain.
Thus, the aim of the invention is to provide for a methods of selection and screening of hDIS3 PIN domain inhibitors, yeast strains and cell lines used in such methods. The aim of the invention is to provide new therapeutic agents—selected hDIS3 PIN domain inhibitors and uses thereof in the treatment of cancers, especially multiple myeloma, bearing mutations in hDIS3 RNB domain, based on synthetic lethality between the mutations present in the RNB domain and the inhibition of endonucleolytic activity of the hDIS3 PIN domain.
The invention relates to a method for selection for the DIS3 PIN domain endonuclease activity inhibitor for use in therapy of cancer cells having the mutation in hDIS3 RNB domain, wherein the method comprises the following steps:
a) construction of a model system comprising DIS3, its fragments, homologues or variants with a mutation in RNB domain, preferably by introducing the mutation occurring in hDIS3 in cancer cells,
b) contacting the model system obtained in a) with a tested agent, a putative DIS3 PIN domain endonuclease activity inhibitor,
c) evaluation of the activity of the PIN domain in the presence and absence of the tested agent, by the way appropriate to the model system,
d) selection of the tested agent for which PIN domain activity is changed as contacted with the tested agent and by this obtaining the DIS3 PIN domain endonuclease activity inhibitor.
The invention also relates to the method for selection for the DIS3 PIN domain endonuclease activity inhibitor for the use in therapy of cancer cells having the mutation in hDIS3 RNB domain, wherein the method comprises:
a) obtaining a purified protein comprising the DIS3 PIN domain, its fragment, variant or homologue, preferably the isolated hDIS3 PIN domain;
b) contacting the protein obtained in a) with a tested agent, a putative DIS3 PIN domain endonuclease activity inhibitor,
c) evaluation of the activity of the PIN domain in the presence and absence of the tested agent, preferably by in vitro digestion of RNA substrates,
d) selection of the tested agent for which PIN domain activity is changed as contacted with the tested agent and by this, obtaining the DIS3 PIN domain endonuclease activity inhibitor.
The method involving the use of purified hDIS3 variants with mutations in hDIS3 RNB domain is a suitable model system for selection and screening for hDIS3 PIN domain inhibitors in vitro.
The invention also relates to the method of selection for a DIS3 PIN domain endonuclease activity inhibitor for the use in therapy of cancer cell having the mutation in hDIS3 RNB domain, wherein the method comprises:
a) construction of a yeast strain comprising Dis3 with the mutation in RNB domain, preferably by introducing the mutation equivalent to the mutation occurring in hDIS3 in cancer cells,
b) contacting the yeast strain obtained in a) with a tested agent, a putative DIS3 PIN domain endonuclease activity inhibitor,
c) evaluation of the activity of the PIN domain in the presence and absence of the tested agent,
d) selection of the tested agent as the DIS3 PIN domain endonuclease activity inhibitor which after treatment of the yeast constructed in a), but not the yeast with wild type Dis3, leads to selective growth retardation or cell death (synthetic lethality).
Method involving the use of yeast strains with mutations in Dis3 RNB domains is a suitable model system for selection and screening for DIS3 PIN domain inhibitors in vivo.
The invention also relates to the method for selection for a hDIS3 PIN domain endonuclease activity inhibitor for the use in therapy of cancer cells having a mutation in hDIS3 RNB domain, wherein the method comprises:
a) construction of an engineered cell line, preferably a human cell line, comprising hDIS3 with a mutation in RNB domain, its fragment or variant, preferably by introducing a mutation occurring in hDIS3 in cancer cells,
b) contacting the cell line obtained in a) with a tested agent, a putative hDIS3 PIN domain endonuclease activity inhibitor,
c) evaluation of the activity of the PIN domain in the presence and absence of the tested agent,
d) selection of the tested agent as a hDIS3 PIN domain endonuclease activity inhibitor, which after treatment of the cell line constructed in a) but not the cell line expressing wild type hDIS3, leads to selective growth retardation or cell death (synthetic lethality).
The method involving the use of engineered human cell lines with mutations in hDIS3 RNB domain is a suitable model system for selection and screening for hDIS3 PIN domain inhibitors in vivo.
In the preferred method for selection for a DIS3 PIN domain endonuclease activity inhibitor according to invention, the mutation in hDIS3 gene in cancer cells is at least one mutation occurring in multiple myeloma, medulloblastoma, acute myeloid leukaemia.
In the preferred method for selection for a DIS3 PIN domain endonuclease activity inhibitor according to invention, the mutation in hDIS3 RNB domain in cancer cells is at least one mutation selected from S477R, G766R, R780K, V504G, A524P, I845V, D487N with relation to SEQ ID NO: 1.
In the preferred method for selection for a DIS3 PIN domain endonuclease activity inhibitor according to invention the hDIS3 has at least one mutation in the coding sequence within its RNB domain of AA 427-843of SEQ ID NO: 1, preferably contains at least one amino acid change within its RNB domain of AA 427-843 of SEQ ID NO: 1.
In the preferred method for selection for a hDIS3 PIN domain endonuclease activity inhibitor the DIS3 PIN domain is hDIS3 PIN of AA 1-217 of SEQ ID NO: 1.
The invention also relates to therapeutic agent for use in the treatment of cancer cell having a mutation in hDIS3 RNB domain, wherein the therapeutic agent is obtained with the method of selction for a DIS3 PIN domain endonuclease activity inhibitor according to invention.
The invention also relates to therapeutic agent 2-hydroxy-(4H)-isoquinoline-1,3-dione (also known as AClLAHYL), its derivatives, resolved enantiomers, diastereoisomers, solvates and pharmaceutically acceptable salts thereof for use in treatment of cancer cell having a mutation in hDIS3 RNB domain.
The preferred therapeutic agent is used for cancer cell having a mutation in hDIS3 RNB domain in the cancer cell with at least one mutation selected from S477R, G766R, R780K, V504G, A524P, I845V, D487N with relation to SEQ ID NO: 1.
The preferred therapeutic agent is used for cancer cell in which the hDIS3 RNB domain is hDIS3 RNB domain with at least one mutation in the sequence AA 427-843of SEQ ID NO: 1.
If the therapeutic agent is a chemical compound the invention also includes solvates, pharmaceutically acceptable prodrugs, pharmaceutically active metabolites, and pharmaceutically acceptable salts of such compounds. With respect to the therapeutic agent 2-hydroxy-(4H)-isoquinoline-1,3-dione the invention also includes derivatives, resolved enantiomers, diastereoisomers, solvates and pharmaceutically acceptable salts thereof having inhibitor activity for DIS3 PIN domain endonuclease activity.
The invention also relates to the use of the therapeutic agent of the invention or the one obtained by the method for selection for a DIS3 PIN domain endonuclease activity inhibitor, for preparation of a medicament for treating cancer cells having a mutation in hDIS3 RNB domain.
The invention also relates to the use of 2-hydroxy-(4H)-isoquinoline-1,3-dione, its derivatives, resolved enantiomers, diastereoisomers, solvates and pharmaceutically acceptable salts thereof for preparation of a medicament for treating cancer cells having a mutation in hDIS3 RNB domain.
The therapeutic agent is preferably used for cancer cell having the mutation in hDIS3 RNB domain with at least one mutation selected from S477R, G766R, R780K, V504G, A524P, I845V, D487N with relation to SEQ ID NO: 1.
Preferably the cancer cell having a mutation in hDIS3 RNB is selected from multiple myeloma, medulloblastoma, acute myeloid leukaemia.
In the preferred use of the therapeutic agent, hDIS3 has at least one mutation in the coding sequence within its RNB domain of AA 427-843 of SEQ ID NO: 1, preferably contains at least one amino acid change within its RNB domain of AA 427-843 of SEQ ID NO: 1.
The invention also relates to the composition comprising a therapeutic agent for use in the treatment of cancer cells having a mutation in hDIS3 RNB domain, wherein the therapeutic agent is obtained with the method for selection for a DIS3 PIN domain endonuclease activity inhibitor of the invention or is therapeutic agent of the invention.
In the preferred embodiment the composition is used for the cancer cell having a mutation in hDIS3 RNB domain in the cancer cell with at least one mutation selected from S477R, G766R, R780K, V504G, A524P, I845V, D487N with relation to SEQ ID NO: 1.
In the preferred use of composition the cancer cell having a mutation in hDIS3 RNB is selected from multiple myeloma, medulloblastoma, acute myeloid leukaemia.
The composition preferably further comprises at least one additional agent having anti-cancer activity, wherein the additional agent has anti-cancer activity against multiple myeloma, medulloblastoma or acute myeloid leukaemia.
Preferably the additional agent is selected from bortezomib, carfilzomib clafen (cyclophosphamide), cyclophosphamide, cytoxan (cyclophosphamide), doxil (doxorubicin hydrochloride liposome), doxorubicin hydrochloride liposome, dox-SL (doxorubicin hydrochloride liposome), evacet (doxorubicin hydrochloride liposome), kyprolis (carfilzomib), lenalidomide, LipoDox (doxorubicin hydrochloride liposome), mozobil (plerixafor), neosar (cyclophosphamide), plerixafor, pomalidomide (pomalyst), pomalyst, revlimid (lenalidomide), synovir (thalidomide), thalidomide, thalomid (thalidomide), velcade (bortezomib), zoledronic acid, zometa (zoledronic acid) for myeloma;
adriamycin PFS (doxorubicin hydrochloride), adriamycin RDF (doxorubicin hydrochloride), arsenic trioxide, cerubidine (daunorubicin hydrochloride), clafen (cyclophosphamide), cyclophosphamide, cytarabine, cytosar-U (cytarabine), cytoxan (cyclophosphamide), daunorubicin hydrochloride, doxorubicin hydrochloride, neosar (cyclophosphamide), rubidomycin (daunorubicin hydrochloride), tarabine PFS (cytarabine), trisenox (arsenic trioxide), vincasar PFS (vincristine sulfate), vincristine sulfate for acute myeloid leukemia (AML);
lomustine, cisplatin, carboplatin, vincristine or cyclophosphamide for medulloblastoma.
In the preferred use of composition DIS3 in cancer cell has at least one mutation in the coding sequence within its RNB domain of AA 427-843 of SEQ ID NO: 1, preferably contains at least one amino acid change within its RNB domain of AA 427-843 of SEQ ID NO: 1.
The present invention also relates to therapeutic agent, its preparations or compositions comprising a therapeutic agent of the invention, or composition of the invention in a pharmaceutically acceptable carrier. The choice of a pharmaceutically acceptable carrier will depend on the administration route, as well as on the solubility of the therapeutic agent and the need to protect it from inactivation or degradation prior to entering the cell, tissue or organism. Pharmaceutically acceptable carriers may comprise solvents, dispersants and additives (coatings, surfactants, flavoring agents, antioxidants and others).
The composition of the invention may also comprise at least one additional agent with an anti-cancer activity, preferably an agent with an activity against multiple myeloma, medulloblastoma or acute myeloid leukaemia. The therapeutic agent of the invention and the additional anti-cancer agent(-s) may be co-administered or administered simultaneously or sequentially or in any suitable order.
The composition and the therapeutic agent of the invention may be administered by various routes, including injection, orally or topically. Preparations for oral administration may be in the form of solutions, suspensions, emulsions, capsules, tablets or powders. Oral liquid preparations may contain diluents such as water and alcohols, optionally with the addition of surfactants, suspending agents or emulsifiers. Capsules can be of the gelatin type, and tablets may contain excipients known in the art. Injectable preparations include aqueous and non-aqueous isotonic sterile solutions, optionally containing antioxidants, buffers, isotonic agents and the like. A typical carrier is water or physiological saline.
A composition is generally obtained by diluting and mixing the active ingredient with the carrier or closing it in a carrier. If the therapeutic agent is a nucleic acid, it can be administered in a special liposomal systems that recognize the type of cells or tissues. In another embodiment, the nucleic acid can be introduced by using an expression vector containing a sequence encoding the desired inhibitor, for example, micro RNA or siRNA.
The dose of the therapeutic agent is determined taking into account the route of administration, the progression of the disease, and other significant circumstances like age and weight of the patient.
The invention also relates to the method for inducing synthetic lethality in a cancer cell, wherein the cancer cell having a mutation in hDIS3 RNB domain is treated with an agent inhibiting the endonucleolytic activity of hDIS3 PIN domain.
In the preferred method for inducing synthetic lethality in cancer cells, the agent inhibiting the activity of hDIS3 PIN domain is obtained by the method for selection for a DIS3 PIN domain endonuclease activity inhibitor or is the therapeutic agent of the invention or is the composition of the invention.
In the preferred method for inducing synthetic lethality in cancer cells DIS3 has at least one mutation in the coding sequence within its RNB domain of AA 427-843 of SEQ ID NO: 1, preferably contains at least one amino acid change within its RNB domain of AA 427-843 of SEQ ID NO: 1.
In the preferred method for inducing synthetic lethality in cancer cells the DIS3 PIN domain is hDIS3 PIN of AA 1-217 of SEQ ID NO: 1.
The invention also relates to the yeast strain, preferably a S. cerevisiae strain, for use in the method of selection for a DIS3 PIN domain endonuclease activity inhibitor, wherein the yeast strain comprises DIS3 with a mutation in Dis3 RNB domain, which is an equivalent of a mutation found in cancer cell, preferably mutation found in medulloblastoma, acute myeloid leukaemia or multiple myeloma cell.
In the yeast strain mutation in Dis3 RNB domain preferably at least one mutation selected from human equivalents S477R, G766R, R780K, V504G, A524P, I845V, D487N with relation to SEQ ID NO: 1 is present.
In the yeast strain the Dis3 has preferably at least one mutation in the coding sequence within its RNB domain, preferably contains at least one amino acid change within its RNB domain which is homologous to human hDIS3RNB domain of AA 427-843 of SEQ ID NO: 1 and therefore such mutations are equivalent to mutation in cancer cell selected from medulloblastoma, acute myeloid leukaemia or multiple myeloma.
In the preferred yeast strain the DIS3 PIN domain is hDIS3 PIN of AA 1-217 of SEQ ID NO: 1 or the DIS3 PIN domain is DIS3 PIN of (AA 1-241 of SEQ ID NO: 2). The preferred yeast strain is selected from dis3-G833R, dis3-R847K, dis3-V568G, dis3-A588P, dis3-D551N.
In the preferred method of selection for a DIS3 PIN domain endonuclease activity inhibitor for use in therapy of cancer cells having a mutation in hDIS3 RNB domain, the method comprises the use of the yeast strain of the invention.
The invention also relates to the use of the yeast strain of invention for the screening for a DIS3 PIN domain endonuclease activity inhibitor for its use in the therapy of cancer cell having the mutation in hDIS3 RNB domain.
The invention also relates to the cell line for use in the method of selection for a DIS3 PIN domain endonuclease activity inhibitor, in which the cell line comprises DIS3 with the mutation in DIS3 RNB domain which is an equivalent of the mutation found in cancer cell.
The cell line is preferably used in the method of selection for a DIS3 PIN domain endonuclease activity inhibitor for the cancer cell selected from medulloblastoma, acute myeloid leukaemia, multiple myeloma.
The preferred cell line is a human cell line, preferably a derivative of the HEK293 Flp-In T-Rex cell line.
The preferred human cell line of the invention, is constructed with the use of a vector comprising a bidirectional inducible promoter expressing:
The invention also provides the method for construction of the engineered cell line according to the invention and vector for use in such a method.
In the preferred cell line the mutation in DIS3 RNB domain at least one mutation selected from S477R, G766R, R780K, V504G, A524P, I845V, D487N with relation to SEQ ID NO: 1 is present.
In the preferred cell line DIS3 RNB codes for hDIS3 RNB with at least one mutation in the sequence AA 427-843 of SEQ ID NO: 1.
In the preferred cell line DIS3 PIN domain is hDIS3 PIN of AA 1-217 of SEQ ID NO: 1.
In the preferred method of selection for a DIS3 PIN domain endonuclease activity inhibitor for use in therapy of cancer cells having a mutation in hDIS3 RNB domain the method comprises the use of cell line of invention.
The invention also relates to the use of the cell line of invention for the screening for a DIS3 PIN domain endonuclease activity inhibitor for its use in the therapy of cancer cell having the mutation in hDIS3 RNB domain.
In another aspect, the present invention provides a method of screening of cancer patients, in particular medulloblastoma, acute myeloid leukaemia, multiple myeloma patients, with cancer cell harboring a hDIS3 mutation, for the susceptibility of cancer cell to inhibitors of hDIS3 PIN domain activity, wherein the method is an in vitro assay using purified hDIS3 protein, its fragments or homologues, harboring the said mutation.
In another aspect, the invention provides a method of screening of cancer patients, in particular medulloblastoma, acute myeloid leukaemia, multiple myeloma patients, with cells harboring a hDIS3 mutation, for the susceptibility of cancer cells to inhibitors of hDIS3 PIN domain activity, wherein the method involves the use of yeast strains with corresponding mutations in Dis3 RNB domain. The invention also provides the yeast strains for use in such a method of screening of cancer patients, The yeast strains according to the invention for use in such a method harbor a non-lethal Dis3 RNB domain mutation, corresponding to the mutation found in human patient.
In yet another aspect, the invention provides the method involving the use of engineered human cell lines with mutations in hDIS3 RNB domain for screening of cancer patients, in particular medulloblastoma, acute myeloid leukaemia, multiple myeloma patients, for the susceptibility of cancer cells to inhibitors of hDIS3 PIN domain activity. The invention also provides the engineered human cell lines for use in such a method for screening of cancer patients. The engineered human cell line used in such a method, harbors a non-lethal Dis3 RNB domain mutation, corresponding to the mutation found in patient.
As discussed herein, the invention relates to the novel methods of selection of exosome complex inhibitors which are hDIS3 PIN domain inhibitors, which can be further use as therapeutic agent for the treatment of cancers bearing mutations in the RNB domain of the hDIS3 ribonuclease. Preferably RNB domain of the hDIS3 is (427-843 of SEQ ID NO: 1), and hDIS3 PIN domain is (1-217 of SEQ ID NO: 1). The mutations in the RNB domain of the hDIS3 ribonuclease are preferably the same as the mutations occurring in cancers like medulloblastoma, acute myeloid leukaemia, and, in particular, multiple myeloma. The mutations in the RNB domain of the hDIS3 ribonuclease are preferably selected from mutations S477R, G766R, R780K, V504G, A524P, I845V with relation to SEQ ID NO: 1. The invention also relates to the novel hDIS3 PIN domain inhibitor which is selected by the method of invention and its use as therapeutic agent for the treatment of cancers bearing mutations in the RNB domain of the hDIS3 ribonuclease, such as medulloblastoma, acute myeloid leukaemia, and, in particular, multiple myeloma. The mutations in the RNB domain of the hDIS3 ribonuclease are preferably the same as the mutations occurring in cancers like multiple myeloma, medulloblastoma, acute myeloid leukaemia The mutations in the RNB domain of the hDIS3 ribonuclease are preferably selected from mutation S477R, G766R, R780K, V504G, A524P, I845V with relation to SEQ ID NO: 1. The use of exosome complex inhibitor which is hDIS3 PIN domain inhibitor as the therapeutic agent is based on synthetic lethality between the mutations already present in the RNB domain and externally-induced inhibition of endonucleolytic activity of the hDIS3 PIN domain by the novel exosome complex inhibitor which is hDIS3 PIN domain inhibitor.
As used herein, the terms “protein”, “peptide”, “protein fragment” are used interchangeably to refer to polymers of amino acid residues of any length. The polymer can be linear or branched, it may comprise modified amino acids or amino acid analogs, and it may be interrupted by chemical moieties other than amino acids. The terms also encompass an amino acid polymer that has been modified naturally or by intervention; for example disulfide bond formation, glycosylation, lipidation, acetylation, phosphorylation, or any other manipulation or modification, such as conjugation with a labeling or bioactive component.
A “protein domain” as used herein, is a conserved part of a given protein sequence and structure that can evolve, function, and exist independently of the rest of the protein chain. Each domain forms a compact three-dimensional structure and often can be independently stable and folded. The “DIS3 RNB domain” as used herein, is a part of the DIS3 ribonuclease, preferably yeast (for example Saccharomyces cerevisiae) or human, with an exoribonucleolytic activity. The hDIS3 RNB domain comprises amino acids 427-843 in the hDIS3 protein sequence (AA 427-843 of SEQ ID NO: 1). The S. cerevisiae Dis3 RNB domain comprises amino acids 478-910 in the yeast Dis3 protein sequence (AA 478-910 of SEQ ID NO: 2).
The “DIS3 PIN domain” as used herein, is a part of the DIS3 ribonuclease, preferably yeast (for example Saccharomyces cerevisiae) or human, with an endoribonucleolytic activity. The DIS3 PIN domain in human cells comprises amino acids 1-217 in the DIS3 protein sequence(AA 1-127 of SEQ ID NO: 1). The S. cerevisiae Dis3 PIN domain comprises amino acids 1-241 in the yeast Dis3 protein sequence (AA 1-241 of SEQ ID NO: 2).
An “isolated” polynucleotide or polypeptide is one that is substantially free of the materials with which it is associated in its native environment.
The term “homology” or “identity”, with respect to a nucleotide or amino acid sequence, indicates a quantitative measure of homology between two sequences. Homologues have at least a 50% identity compared to a reference sequence. Preferably a homologue has 80, 85, 90, 95, 98 or 99% identity to a sequence of reference. A “homologous nucleic acid sequence” or “homologous amino acid sequence,” or variations thereof, refer to sequences characterized by a homology at the nucleotide level or amino acid level as defined above. Sequence similarity for polypeptides, which is also referred to as sequence identity, is typically measured using sequence analysis software. In the present invention, homologous protein sequences include protein sequences of a polypeptide of species other than humans, including, but not limited to, yeast (Saccharomyces cerevisiae).
As used herein, the terminology “equivalent mutation” refers to mutations in substantially equivalent sequences, for example homologues as defined above, that are in locations different from, but corresponding to one another.
“Synthetic lethality” used herein is intended to refer to a type of interaction where the co-occurrence of a genetic event and the activity of an agent (like therapeutic agent, DIS3 PIN domain endonuclease activity inhibitor or drug) results in organismal or cellular death or selective growth retardation, in particular cancer cell death.
A “vector” refers to a recombinant DNA or RNA plasmid or virus that comprises a heterologous polynucleotide to be delivered to a target cell, either in vitro or in vivo.
The term “recombinant” means a polynucleotide semisynthetic, or synthetic origin which either does not occur in nature or is linked to another polynucleotide in an arrangement not found in nature.
The terms “cancer” and “cancerous” refer to or describe the physiological condition in mammals that is typically characterized by unregulated cell growth. A “tumor” comprises one or more cancerous cells. Examples of cancers include, but are not limited to, multiple myeloma, medulloblastoma, acute myeloid leukaemia. Thus by the term cancer as use herein both cancer and tumor should be understood.
The phrase “pharmaceutically acceptable” indicates that the substance or composition must be compatible chemically and/or toxicologically, with the other ingredients comprising a formulation, and/or the mammal being treated therewith.
As used herein, the terms “treat” and “treatment” refer to both therapeutic treatment and prophylactic or preventative measures, wherein the object is to prevent or slow down (lessen) an-undesired physiological change or disorder, such as the development or spread of cancer. For purposes of this invention, beneficial or desired clinical results include, but are not limited to, alleviation of symptoms, diminishment of extent of disease, stabilized (i.e., not worsening) state of disease, delay or slowing of disease progression, amelioration or palliation of the disease state, and remission (whether partial or total), whether detectable or undetectable. “Treatment” can also mean prolonging survival as compared to expected survival if not receiving treatment. Those in need of treatment include those already with the condition or disorder as well as those prone to have the condition or disorder or those in which the condition or disorder is to be prevented. In the case of cancer, the treatment should be understood as reducing the number of cancer cells; reducing the cancer (tumor) size; inhibiting (i.e., slowing to some extent and preferably stopping) cancer cell or tumor growth; and/or relieving to some extent one or more of the symptoms associated with the cancer.
The term “derivative” for chemical compound shall be understood as compound that is derived from a similar compound by some chemical or physical process.
The majority of mutations found in MM and other cancers affect conserved residues in the hDIS3 RNB domain which points to the fact that they may have an impact on the exonucleolytic activity of this enzyme (FIG. IA). On the contrary, none of these mutations locate within PIN domain, entirely responsible for the second, endoribonucleolytic activity of hDIS3 (
The applicants have found that several mutations in the hDIS3 gene, observed in MM patients, like the hDIS3S477R, hDIS3G766R and hDIS3R780K variants, significantly diminish exoribonucleolytic activity of hDIS3 protein (
In support of this, the applicants further demonstrate that yeast strains expressing endogenous Dis3 with introduced amino acid changes equivalent to those found in MM patients (such as: dis3-G833R (G766R in humans), dis3-R847K (R780K in humans), dis3-V568G (V504G in humans) and dis3-A588P (A524P in humans)), display growth defects of various strength. This is accompanied by molecular phenotypes typical for exosome dysfunction (
It turned out that the inactivation of hDIS3 PIN domain endonucleolytic activity acts synergistically with MM-associated mutations within RNB domain, causing enhanced growth aberrations and additional accumulation of PROMPTs (
Further, it was shown by the applicants that 2-hydroxy-(4H)-isoquinoline-1,3-dione (AClLAHYL) acts as an inhibitor of the hDIS3 PIN domain activity and is capable of specifically inhibiting the growth of cells with DIS3 MM-associated mutations.
Therefore, an exosome complex inhibitor, preferably a hDIS3 PIN domain inhibitor, in particular 2-hydroxy-(4H)-isoquinoline-1,3-dione (AC1LAHYL), its derivatives, resolved enantiomers, diastereoisomers, solvates and pharmaceutically acceptable salts thereof may be used to specifically target cancer cells, such as medulloblastoma, acute myeloid leukaemia and, in particular, multiple myeloma cells, bearing the hDIS3 mutations. The mutations in the RNB domain of the hDIS3 in these cancers are preferably selected from mutation S477R, G766R, R780K, V504G, A524P, I845V with relation to SEQ ID NO: 1.
The in vitro assay, based on the in vitro digestion of RNA substrates, described herein, may also be used for screening for novel hDIS3 PIN domain endonuclease activity inhibitors, with the use of purified hDIS3 protein, its fragments (such as a recombinant version of the isolated PIN domain) or homologs. The purified hDIS3 protein or its isolated PIN domain may be obtained and purified from bacteria, preferably E. coli, following heterologous expression. Preferably, the isolated hDIS3 PIN domain comprises the sequence disclosed in 1-217 of SEQ ID NO: 1. The RNA substrate may be for example 5′ fluoresceine-labeled ss17-(A)34. The evaluation of the activity of the PIN domain in the presence and absence of the tested agent may be evaluated by any means readily known to the ones skilled in the art, such as denaturing PAGE and fluorimaging. Therapeutic agents found with the use of this method may preferably be used in cancer therapy of cancers bearing hDIS3 mutations, preferably mutations in the RNB domain of the hDIS3 such as multiple myeloma, medulloblastoma, acute myeloid leukaemia.
As described herein, the yeast strains expressing endogenous Dis3 with introduced amino acid changes equivalent to those found in MM patients, such as dis3-G833R (G766R in humans), dis3-R847K (R780K in humans), dis3-V568G (V504G in humans), dis3-A588P (A524P in humans), may also be applied for screening for novel therapeutic agents which are hDIS3 PIN domain inhibitors. Such agents may be detected by their capability to inhibit the growth of said yeast strains when added to the yeast growth medium. The exosome function may also be evaluated for such yeast strains and compared for yeast grown in the presence and absence of the tested agent. The evaluation of exosome function may be performed by any means known to the ones skilled in the art, for example by isolation of total RNA, further northern-blot analysis using probes specific to typical exosome substrates; such as 5′-ETS, NEL025 cryptic unstable transcript and 7S precursor of 5.8S rRNA synthesis (as well as their degradation intermediates). Therapeutic agents found with this method may preferably be used in cancer therapy of cancers bearing hDIS3 mutations, preferably mutations in the RNB domain of the hDIS3 such as multiple myeloma, medulloblastoma, acute myeloid leukaemia.
The engineered human cell line assay, described herein, may also be used for screening for novel PIN domain endonuclease activity inhibitors. The human model stable cell lines producing hDIS3 variants for example with MM-like mutations may be obtained with the use of a vector for transfection, said vector comprising a bidirectional inducible promoter expressing for example:
The vector may additionally comprise a marker gene, such as eGFP. The vector of the invention may comprise the nucleotide sequence of pMM9 or pMM10.
This type of vector allows for the construction of a human cell line expressing a desired hDIS3 variant. Such an engineered human cell line of the invention may be used for the evaluation of exosome function by any means known to the ones skilled in the art. Such methods may include, for example: assessing cellular growth of the cells after induction of expression of miRNA silencing the endogenous copy of hDIS3 and induction of expression of the exogenous hDIS3 variant. This may be accomplished by microscopy (preferably fluorescence microscopy) and/or flow cytometry. It may also be coupled with cell viability assessment (preferably an assay sensitive to cell metabolic activity, for example AlamarBlue®. The evaluation of exosome function may include examining exosome substrates, such as 5.8S processing intermediates, tRNAs, RNA polymerase III transcripts and PROMPTs. This may be achieved, for example, by total RNA isolation after induction of expression of sh-miRNA silencing of the endogenous hDIS3 copy and simultaneous induction of expression of the exogenous hDIS3 variant. This may be followed by low- or high-resolution northern blots. Isolated RNA may also be reverse transcribed to DNA and further analyzed with methods such as PCR or RealTime PCR.
The in vitro assay disclosed herein, based on RNA substrates and purified hDIS3 protein bearing the mutation found in specific cancer patients, may also be used for evaluating the effects on exosome functions of new hDIS3 mutations found in the cancer patients and further to assess the potential susceptibility of the cancer cells with the specific mutation to future therapy using an exosome complex inhibitor, preferably a hDIS3 PIN domain inhibitor. The purified hDIS3 protein bearing the mutation found in specific cancer patients may be prepared as a recombinant variant of wild type hDIS3 sequence and purified from a bacteria, preferably E. coli, following heterologous expression. The RNA substrates may be for example an in vitro transcribed RNA substrate uniformly labeled with UTP, or as another example, 5′-end labeled RNA substrates (such as used in Example 1, below).
The evaluation of the activity of the hDIS3 protein bearing the mutation found in specific cancer patients in presence and absence of the tested agent may be evaluated by any means readily known to the ones skilled in the art, such as: TLC, or denaturing PAGE and phosphorimaging, depending on labeling of RNA substrates.
The yeast methods described herein, based on yeast strains expressing endogenous Dis3 with introduced amino acid changes equivalent specifically to those found in cancer patients, may also be used for evaluating the effects on exosome functions of new hDIS3 mutations found in the cancer patients and further to assess the potential susceptibility of the cancer cells with the specific mutation to future therapy using an exosome complex inhibitor, preferably a hDIS3 PIN domain inhibitor. The evaluation of exosome function in such mutant yeast strains may be performed by any means known to the ones skilled in the art, for example by isolation of total RNA, further northern-blot analysis using probes specific to typical exosome substrates; such as 5′-ETS, NEL025 cryptic unstable transcript and 7S precursor of 5.8S rRNA synthesis (as well as their degradation intermediates).
The vector of the invention may also be used to obtain an engineered human cell line for a specific mutation found in patient's cancer cells in order to evaluate the exoribonucleolytic activity of hDIS3 protein for the specific variant and further to assess the potential susceptibility of the cancer cells with the specific mutation, to therapy using an exosome complex inhibitor, preferably a hDIS3 PIN domain inhibitor. Such an engineered human cell line may be used for evaluation of exosome function by any means known to the ones skilled in the art. Such methods may include, for example: assessing cellular growth of the cells after induction of expression of miRNA silencing the endogenous copy of hDIS3 and induction of expression of the exogenous hDIS3 variant. This may be accomplished by microscopy (preferably fluorescence microscopy) and/or flow cytometry. It may also be coupled with cell viability assessment (preferably an assay sensitive to cell metabolic activity, for example AlamarBlue®. The evaluation of exosome function may include examining exosome substrates, such as 5.8S rRNA processing intermediates, tRNAs, RNA polymerase III transcripts and PROMPTs. This may be achieved for example by total RNA isolation after induction of expression of sh-miRNA silencing of the endogenous hDIS3 copy and simultaneous induction of expression of the exogenous hDIS3 variant. This may be followed by low- or high-resolution northern blots. Isolated RNA may also be reverse transcribed to DNA and further analyzed with methods such as PCR or RealTime PCR.
Although the present invention and its advantages have been described in detail, it should be understood that various changes, substitutions and alterations can be made herein without departing from the spirit and scope of the invention as defined in the appended claims.
The present invention will be further illustrated in the following examples which are given for illustration purposes only and are not intended to limit the invention in any way.
In the following examples, unless specified otherwise, standard materials and methods are used as described in Sambrook J. et al. “Molecular Cloning: A Laboratory Manual, 2nd edition, 1989, Cold Spring Harbor, N.Y. Cold Spring Harbor Laboratory Press, or proceeding in accordance with manufacturers' recommendations for specific materials and methods.
Bacterial Strains
The following E. coli strains were used: MH1 (E. coli araD lacX74 galU hsdR hsdM rpsL), dam-/dcm-(New England Biolabs; E. coli ara-14 leuB6fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10) TetS endA1 rspL136 (StrR) dam13::Tn9 (CamR) xylA-5 mtl-1 thi-1 mcrB1 hsdR2), BL21-CodonPlus-RIL (Stratagene; E. coli B F− ompT hsdS(rB− mB−) dcm+ Tetr gal endA Hte [argU ileY leuW Camr]).
Yeast Strains
S. cerevisiae strain encoding fusion of wild-type Dis3p with and protein A tag-TEV protease cleavage site and TRP selection marker (ADZY532; referred herein to as wt) was a derivative of haploid BMA64 (MATa ade 2-1 his3-11,15 leu2-3, 112 trp1Δ ura3-1 can1-100), created by homologous recombination (Puig et al. 2001). S. cerevisiae strain dis3-D551N mutant was constructed previously (Dziembowski et al. 2007). rrp6Δ strain was from Euroscarf (acc. no.: Y11777; BY4742 MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 YOR001w::kanMX4). S. cerevisiae strains dis3-V568G, dis3-A588P, dis3-G833R and dis3-R847K mutants were constructed as specified in Example II.
S. cerevisiae strain ADZY531 was constructed as follows. D171N mutation was introduced into the DIS3 gene in the BMA64 wild type haploid strain by in vivo recombination. First, the fragment of DIS3 gene with the D171N mutation was amplified using ADZKD 106 (CGGTCATATGAGAGTGTGTTGCG) (SEQ ID NO: 3) and ADZKD110 primers (GAGAATTTGTATTTTCAGGGTGAGC) (SEQ ID NO: 4) and pBS3278 vector (Lebreton et al. 2008) as a template. The remaining part of the DIS3 gene, together with protein A tag-protease TEV cleavage site and TRP selection marker was amplified using ADZKD111 (GCTCACCCTGAAAATACAAATTCTC) (SEQ ID NO: 5) and ADZKD107 (AGTGGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCG) (SEQ ID NO: 6) primers and a genomic DNA isolated from ADZY532 strain as a template. In the last step, overlap PCR was performed using ADZKD106 and ADZKD107 primer and both amplicons obtained in reactions described above, in order to join both fragments. Transformation of the haploid BMA64 strain with the final PCR product gave strain ADZY531.
All restriction enzymes and T4 DNA ligase were from Thermo Scientific. CIP was from New England Biolabs. All DNA purification kits: DNA Plasmid Mini, DNA Plasmid Midi and
Gel-Out were from A&A Biotechnology. T4 PNK was from New England Biolabs. PCR reactions were performed with the Phusion DNA polymerase (Thermo Scientific).
In the Table 6 there are gathered hDIS3 RNB domain mutations, their equivalents in yeast strains or cell lines and effects observed for such mutants, obtained in the below Examples.
Proteins Encoded by some hDIS3 Gene Versions with MM-Associated Mutations Display Various Defects in the Degradation of Different RNA Substrates.
Oligonucleotides, Plasmids and Cloning.
The E. coli BL21-CodonPlus-RIL strain was used. Plasmids pMM1, pMM2, pMM3, pMM4, pMM5 and pMM6 (for heterologous expression of various hDIS3 versions in E. coli) were generated by site-directed mutagenesis with oligonucleotide pairs shown in Table 1, using a pHEX1 construct (encoding wild-type hDIS3; Tomecki et al. 2010b) as template.
The presence of S477R, V504G, A524P, G766R, R780K and I845V mutations was confirmed by digestion with PstI, MlsI, XmaJI, BshTI, Psp1406I and Eco1471 restriction enzymes, respectively and sequencing of hDIS3 inserts using primers (Table 2).
pHEX8 construct, encoding hDIS3 with D487N mutation—a genuine catalytic mutant of RNB domain (Tomecki et al. 2010b) was also used for comparison.
Heterologous Expression and Purification of hDIS3 Proteins.
For purification of different hDIS3 versions, E. coli BL21-CodonPlus-RIL strain (Stratagene) was transformed with appropriate plasmids (pHEX1, pHEX8, pMM1, pMM2, pMM3, pMM4, pMM5, pMM6). Transformant growth and further protein isolation was performed according to standard procedures. Proteins were purified from native extracts by nickel affinity chromatography and gel filtration on Superdex S-200 10/300 GL column (GE Healthcare). All steps were done automatically using an ÄKTAxpress apparatus. The purity of the proteins was assessed by electrophoresis in SDS-PAGE gels. Protein amounts were estimated by densitometric analysis of the gels stained with Coomassie Brilliant Blue R-250 using serial dilutions of BSA as a standard. The obtained purified hDIS3 variants corresponding to wild type, catalytic mutant and variants found in MM patients were used further in in vitro biochemical assays.
Substrate Preparation for in vitro Biochemical Assays.
Oligoribonucleotides:
were purified by electrophoresis, excision and elution 5′-end labeling of substrates was performed with T4 PNK (NEB) and [γ-32P]ATP (GE Healthcare). All labeled single-stranded RNA substrates were further purified following electrophoresis. ds17-(A)14 partial RNA duplex was prepared by mixing nonradioactive single-stranded oligoribonucleotides ss17-(A)14 and compl in a 1:1.2 molar ratio, with addition of radiolabeled ss17-(A)14 oligo in standard conditions. Internally labeled RNA was obtained by in vitro transcription performed using pLsml plasmid digested with Sall as a template, in the presence of [α-32P]UTP and T7 RNA polymerase (NEB). Radiolabeled transcript was extracted and further purified by centrifugation in Spin Modules (MP Biomedicals) packed with Sephadex G-50 (Bio-Rad).
pLsml plasmid, used for preparing internally labeled RNA, kindly provided by Prof. Joanna Kufel (University of Warsaw), is pGEM-T Easy with insert encompassing nucleotides 1-387 of the open reading frame corresponding to A. thaliana Lsml protein.
Exoribonuclease Assays.
In vitro enzymatic assays of hDIS3 exoribonucleolytic activity were performed in 10 mM Tris-HCl, pH 8.0; 75 mM NaCl; 1 mM 2-mercaptoethanol, 100 μM MgCl2. Protein concentration was 0.1 μM, while substrate concentration was 0.2 μM or 2 μM for duplex and unstructured RNA molecules, respectively. For gel-based analyses, reactions were performed at 37° C. for the indicated times and then terminated by formamide loading dye Reaction products were resolved in denaturing 20% polyacrylamide, 8 M urea, 1xTBE gels. In the case of TLC-based experiment, samples were collected by mixing aliquots of the reaction mixtures with 1 μl of 0.5 M EDTA and subsequently analysed by running them on PEI-cellulose plates (Schleicher & Schuell) in 0.5 M LiCl/1M formic acid, using non-radioactive UMP as a marker. For both types of analyses, reaction products were visualized using a FUJI Phosphorlmager.
In order to analyze the exonucleolytic activity, recombinant versions of hDIS3 protein (either wild-type or bearing different mutations detected in MM) were purified from E. coli following heterologous overexpression. hDIS3S477R, hDIS3V504G, hDISG766R, hDIS3R780K and hDIS3I845V mutant proteins were successfully purified (
Then, a preliminary biochemical experiment was performed using all purified hDIS3 variants and in vitro transcribed RNA substrate uniformly labeled with UTP. Time-course-based analysis of degradation of such RNA substrate by a standard TLC method allowed to tentatively assess the influence of individual mutations on exoribonucleolytic activity. It was noted that while the degradative potential of the protein was almost unchanged in the case of hDIS3V504G and hms3I845V(i.e. the rate of RNA decay was comparable to hDIS3WT), the activity was severely reduced for hDIS3S477R, hDIS3G766R and hDIS3R780K variants (
In order to assess defects in RNA degradation caused by different hDIS3 mutations in relation to the wild-type protein in a more qualitative way, biochemical assays were performed on various 5′-end labeled oligoribonucleotide substrates, i.e. 1) single-stranded RNA oligonucleotide, composed of 17-mer generic sequence, followed by 14-nucleotide-long oligo(A) tail (5′P*-ss17-(A)14); 2) 44-mer, lacking oligo(A) extension (5′P*-ss44) and 3) structured substrate containing a 17-nucleotide duplex followed by a 14-nt-long oligo(A) tail (5′P*-ds17-(A)14), and decay products were analyzed in denaturing polyacrylamide gels (
Concluding, results of biochemical assays performed employing different techniques and using various RNA substrates strongly indicated that mutations in hDIS3 gene leading to the following amino acid substitutions: S477R, G766R and R780K cause significant aberrations of hDIS3 exoribonucleolytic activity, while in the case of V504G and I845V the effects are more subtle, if any.
Mutations in Yeast DIS3 Gene Corresponding To those Found in MM Patients Cause Cell-Growth Defects and Molecular Phenotypes Leading To an Impairment of the Exosome Function.
S. Cerevisiae Strains (Yeast Strains) and Construction of Mutants.
dis3-V568G, dis3-A588P, dis3-G833R and dis3-R847K mutants (see
Total genomic DNA isolated from ADZY532 (wt) strain served as template in all above-mentioned amplifications. The full-length amplicons with desired mutations were obtained with ADZKD106 and ADZKD107 primers in overlap PCR (where products of the 5′ and 3′ amplifications served as templates), and were subsequently used for transformation. Selected transformants were sporulated and spores were dissected. A spore harboring dis3-V568G mutation gave strain ADZY679, dis3-A588P-ADZY681, dis3-G833R-ADZY783 and dis3-R847K-strain ADZY685. The presence of V568G, A588P, G833R and R847K mutations was validated by amplification of the genomic DNA fragments using ADZKD145 (GGATGATGTTAATTGCTTGG) (SEQ ID NO. 37)—ADZKD146 (TTGAAACTCTACCACCGACC) (SEQ ID NO. 38) primer pair, followed by digestion of PCR products with MlsI, ApaI, BshTI and Psp1406I restriction enzymes, respectively. Finally, correctness of the ORF sequence was confirmed by sequencing of the obtained PCR product, using primers:
rrp6Δ dis3-G833R and rrp6Δ dis3-R847K strains were obtained through crossing rrp6Δ strain with either ADZY783 or ADZY685. Spores were dissected and double mutants were selected. Spores harboring RRP6 deletion together with either DIS3 G833R or R847K mutation gave strains ADZY732 and ADZY742, respectively.
Diploid strains harboring mutations in both PIN and RNB domains of Dis3 - dis3-D171N G833R-pA/DIS3 WT (ADZY713) and dis3-D171N R847K-pA/DIS3 WT (ADZY716), were obtained as follows. Diploid BMA64 strain was transformed with a DNA fragment containing DIS3 gene harboring D171N mutation (mutation in PIN domain) and G833R or R847K mutation (mutation in RNB domain), respectively, a tag (encompassing TEV protease cleavage site and sequence encoding protein A) and the TRP selection marker. First, two parts of the DIS3 gene were amplified: 5′—using ADZKD106-ADZKD141 (CCTAAATAGAGCATTCAACGGTGACCAGG) (SEQ ID NO. 46). primer pair and total genomic DNA isolated from ADZY531 strain (with the DIS3 locus containing D171N mutation in the PIN domain) as a template; and 3′—with ADZKD142 (CCTGGTCACCGTTGAATGCTCTATTTAGG) (SEQ ID NO. 47).—ADZKD107 primer pair, employing total genomic DNA isolated from ADZY783 or ADZY685 (with the DIS3 locus containing G833R or R847K mutation), respectively, as template. The full-length amplicons with the desired mutations were obtained with ADZKD 106 and ADZKD 107 primers in overlap PCR (where products of the 5′ and 3′ amplifications served as templates), and were subsequently used for transformation. Selected transformants gave desired strains.
Yeast Growth Assays.
Yeast strains described above were grown in complete YPD medium at 30° C. overnight until OD600 reached 0.2 before spotting serial dilutions onto YPD plates. Cell growth was analyzed after 60 h of incubation at 25° C., 30° C. or 37° C. Analysis of viability for strains harboring mutations in both PIN and RNB domains of Dis3 was carried out by sporulation of ADZY713 and ADZY716 diploid strains, followed by tetrad dissection.
Rna Isolation and Northern-Blot Analysis.
RNA was isolated from yeast cultures using standard hot acid phenol procedure Northern blots were handled according to standard procedures and probed either at 42° C. (5′-labeled oligonucleotide probes) or at 63° C. (DNA fragments labeled by random-priming).
For detection of yeast 5′-ETS and NEL025 CUT, PCR probes labeled by random-priming with α-32P[dATP] and DecaLabel DNA Labeling Kit (ThermoScientific), as described in Lebreton et al. 2008 were employed. For other transcripts, 32P-labeled oligonucleotides were used as probes. Sequences of these oligonucleotides were the following: yeast 7S antisense oligo—GGCCAGCAATTTCAAGTTA (SEQ ID NO. 48), yeast 5.8S antisense oligo—GCGTTGTTCATCGATGC (SEQ ID NO. 49), yeast 5S antisense oligo—CTACTCGGTCAGGCTC (SEQ ID NO. 50). After hybridization membranes were washed and exposed to Phosphorlmager screens (FujiFilm), which were scanned following exposure using a FLA 7000 scanner (FujiFilm).
Western Blotting.
Protein samples from yeast cultures were run in 10-12% SDS-PAGE gels and immobilized on Protran nitrocellulose membranes (Whatman) Protein A tag was detected directly by using rabbit Peroxidase-Anti-Peroxidase Soluble Complex antibody (Sigma-Aldrich; P1291) (1:3000). Eventually, blots were developed in Curix 60 machine (AGFA), using Immun-Star™ WesternC™ Kit (Bio-Rad) and CL-XPosure™ Films (Thermo Scientific).
The functional analyses of hDIS3 MM mutations were started with the construction of yeast strains expressing endogenous Dis3 with introduced equivalent amino acid changes. It was possible to produce all mutants except dis3-S541R (S477R in humans). First, basic growth tests were performed and it was noticed that dis3-G833R (G766R in humans) and dis3-R847K (R780K in humans) strains display a strong growth retardation phenotype in comparison with the wild-type control, even at physiological temperature (
Then, the molecular effects of DIS3 gene mutations in yeast were tested by northern-blot analyses on total RNA samples isolated from mutant and control strains, using probes detecting typical exosome substrates: 5′-ETS by-product of ribosomal RNA processing, NEL025 CUT (cryptic unstable transcript) and 7S precursor in 5.8S rRNA maturation pathway. It was observed that all DIS3 mutations (genuine D551N catalytic one and four corresponding to those found in MM patients) cause comparable accumulation of full-length 5′-ETS and 7S rRNA with regard to their amounts in wild-type strain (
The observed phenotypes are not due to the lack of expression of the respective DIS3 mutants, as confirmed by western blotting against the protein A tag which was introduced at the 3′-end of all inserts employed during generation of the strains (
Combining DIS3 G833R or R847K mutation with deletion of RRP6 gene did not seem to remarkably enhance the analyzed molecular phenotypes (maybe except additional accumulation of NEL025 CUT in rrp6Δ dis3-G833R in comparison with dis3-G833R single mutant) (
Altogether, experiments performed using the yeast model showed that changing amino acids in Dis3 positions analogous to those cancers in which hDIS3 activity is changed, especially in mutations in RNB domain being equivalent of mutations observed in multiple myeloma patients inhibits S. cerevisiae growth through the impairment of the exosome ability to exonucleolytically degrade its physiological substrates.
MM-Associated hDIS3 Mutations Result in Accumulation of Different RNA Species in Human Cellular Model.
A multistep cloning procedure for generation of vectors for co-expression of different versions of recoded hDIS3 with FLAG epitope at the C-terminus and sh-miRNAs directed against endogenous hDIS3 mRNA was the following. First, inserts encompassing open reading frames coding for WT, RNB MUT (D487N), G766R and R780K variants of hDIS3 were amplified with D3FMluI: (ATATACGCGTGCCGCCACCATGCTCAAGTCCAAGACGTTC) (SEQ ID NO. 51) and D3RB120I: (GCGCGGGCCCTTACTTGTCGTCGTCGTCCTTGTAATCTAT ATCTTTTCCAAGCTTCATCTTCT) (SEQ ID NO. 52) primer pair and using pHEX1, pHEX8, pMM4 or pMMS constructs as respective templates. Next, the inserts were cloned into MluI and Bsp120I sites of BI-16 vector (Sammarco and Grabczyk, 2005), thus replacing hRLUC ORF present therein, with the use of E. coli MH1 strain. This way, [BI-16′] hDIS3 WT, [BI-16′] hDIS3 RNB MUT, [BI-16′] hDIS3 G766R and [BI-16′] hDIS3 R780K transitory vectors were constructed. hDIS3 inserts were sequenced as above.
In parallel, a search was performed for miRNA sequences that should specifically and efficiently target endogenous hDIS3 mRNA, using BLOCK-iT™ RNAi Designer tool from Invitrogen (with “miR RNAi” option) (http://rnaidesigner.invitrogen.com/rnaiexpress). Candidate sequences were chosen, ranked as 1., 3., 5., 6. and 7. (with the highest scores returned by the program; sequences ranked as 2. and 4. were rejected, as they partially overlapped with 1. and 3.), starting at positions 495., 898., 1159., 1273. and 1404. of hDIS3 ORF, respectively. Basing on the general idea of BLOCK-iT™ Pol II miR RNAi Expression Vector Kits from Invitrogen (http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/rnai/Vector-based-RNAi/Pol-II-miR-RNAi-Vectors.html), then a synthetic DNA fragment was designed, encompassing a combination of miRNA sequences listed above miR1159-miR1273-miR1404 (tri-miR2) which was utilized in further experiments. It encoded three tandemly positioned shRNAs corresponding to pre-designed miRNAs—so-called sh-miRs, where sense and antisense miRNA sequences were separated by the loop element enabling formation of the hairpin. Each of the sh-miR sequence was flanked at both termini with motifs ensuring correct miRNA processing from the artificial pre-miRNA precursor, following a natural miRNA biogenesis pathway active in human cells. In addition, polyadenylation signal derived from the gene encoding herpes simplex virus thymidylate kinase (HSV-TK-pA) was placed at the 3′-end of this synthetic cassette, allowing for correct termination of transcription in human cells. The cassette contained EcoRI/Sall and ClaI/HindIII restriction site combinations at the 5′ and 3′ extremities, respectively, which were used in subsequent cloning steps. It was synthesized by BlueHeronBio and inserted between EcoRI and HindIII sites of pUCAmpMinusMCS vector. Next, a sequence encoding eGFP (allowing for monitoring of expression of the cassette containing artificial pre-miRNA) was amplified in PCR using
primer pair and pEGFP-N1 plasmid (Clontech) as a template and inserted into EcoRI and SalI sites of the provided [pUCAmpMinusMCS] tri-miR 2 plasmid, thus giving [pUCAmpMinusMCS] eGFP-tri-miR 2 transitory construct. Additionally, a site recognized by XmaJI restriction endonuclease was introduced in eGFPFor oligonucleotide upstream the 5′-end of eGFP ORF, which was used at further cloning stage.
In the next phase of construct generation, it was necessary to change the sequence of exogenous hDIS3 ORF in order to make it insensitive to miRNA action. To this end, a synthesis of recoded hDIS3 fragment encompassing nucleotides 451.-1457. of the open reading frame was ordered. It is worth noting that this fragment covered all five sites that were initially aimed to be targeted with miRNA, and—on the other hand—it was located outside the region where D487N (RNB MUT), G766R and R780K mutations had been earlier introduced. The idea of recoding was to introduce synonymous mutations into all possible codons (at those positions where degeneration of genetic code could be utilized) within the fragment containing sites recognized by miRNA and taking codon usage frequency into account, so that the sequence would be as much divergent from the initial one as possible. Recoded hDIS3 ORF fragment was synthesized by BlueHeronBio, also as an insert in pUCAmpMinusMCS vector, surrounded with ˜30-45 nt-long flanking regions fully complementary to the initial sequence and terminated at both extremities with sites recognized by SchI restriction enzyme—an endonuclease cleaving DNA at some distance from its site and leaving blunt ends following cleavage. Owing to the presence of such termini, the insert could be excised from the provided [pUCAmpMinusMCS] rec13 hDIS3 plasmid (propagated in E. coli MH1 strain) with SchI and then utilized as a “megaprimer” in overlap extension PCR (Bryksin and Matsumura, 2010), employing [BI-16′] hDIS3 WT, [BI-16′] hDIS3 RNB MUT, [BI-16′] hDIS3 G766R and [BI-16′] hDIS3 R780K plasmids generated in the first step (see above) as templates. Products were digested with DpnI restriction enzyme and introduced to E. coli MH1 strain by transformation. This gave rise to [BI-16′] hDIS3rec WT, [BI-16′] hDIS3rec RNB MUT, [BI-16′] hDIS3rec G766R and [BI-16′] hDIS3rec R780K transitory constructs, respectively. The presence of recoded fragment was checked by digestion with Adel restriction enzyme and sequencing, using primers: HD3F1848, HD3F2429, hD3r8l9R-CTTGTTCTCCTCGGAATCTC (SEQ ID NO: 55), HD3R1592 and HD3R2443.
The aim of the ultimate cloning stage was to transfer a DNA fragment containing a co-cistron of eGFP coding sequence and pre-miRNA/HSV-TK-pA from [pUCAmpMinusMCS] eGFP-tri-miR 2 construct to each of the four BI-16 vector derivatives from the previous step, through replacement of the FLUC ORF present in the latter. To this end, all plasmids were propagated in E. coli dam-/dcm-strain prior to the standard cloning procedure, utilizing XmaJI and ClaI restriction sites, followed by transformation of the ligation products into E. coli MH1 strain. This eventually led to the generation of final constructs: pMM7, pMM8, PMM9, pMM10. Both hDIS3rec and eGFP-tri-miR 2 inserts were sequenced using primers: HD3F1848, HD3F2429, hD3r8l9R, HD3R1592 and HD3R2443, BI16seq1-CATTCTCCGCTCCATCGTTC (SEQ ID NO: 56) and BI16seq2-TCCACTGGTCGACTCACTAC (SEQ ID NO: 57).
Cell Culture and Generation of Stable Cell Lines.
HEK293 Flp-In T-REx (Invitrogen) cells were cultured as monolayers in Dulbecco's modified Eagle's medium (D-MEM, Gibco), supplemented with 10% tetracycline-free foetal bovine serum (TET System Approved FBS, Clontech) and antibiotics (Penicillin-Streptomycin; Sigma-Aldrich) at 37° C. in a 5% CO2 humidified atmosphere. The stable inducible HEK293 Flp-In T-REx cell lines were obtained in this study using the Flp-In™ T-REx™ system (Invitrogen), with the use of pMM7, pMM8, PMM9, pMM10 constructs (highly pure Midi preps of plasmid DNA), were grown in the same conditions as above, supplemented with hygromycin B (100 μg/ml) and blasticidin (10-15 μg/ml) (both from Invitrogen). Transfections were done with Lipofectamine2000 (Invitrogen). Expression of exogenous genes was induced by addition of doxycycline to the culture medium at the final concentration of 100 ng/ml.
RNA Isolation and Northern-Blot Analysis.
RNA was isolated from human cell lines using TRI Reagent (Sigma-Aldrich). and followed by RNA immobilization to membrane Northern blots were handled according to standard procedures.
Randomly-primed PCR products, obtained with the use of primer pairs:
were used as probes for detection of GAPDH mRNA and 7SL RNA, respectively. Randomly-primed probe, generated as described in (Tomecki et al. 2010b; Supplementary Information) was used for detection of hDIS3 transcript. Randomly-primed DNA probe specific to histone H2A mRNA was generated by EcoRI/HindIII-mediated excision of sequence corresponding to its full-length CDS (GenBank ID: AY131971.1), cloned between respective sites of pUC19; the construct was kindly provided by Prof. Zbigniew Dominski. For other transcripts, 32P-labeled oligonucleotides were used as probes. Sequences of these oligonucleotides were according to Table 4.
32P-labeled oligonucleotides used as probes.
After hybridization membranes were washed and exposed to PhosphorImager screens (FujiFilm), which were scanned following exposure using a FLA 7000 scanner (FujiFilm).
Western Blotting.
Protein samples from human cell lines were treated as described in Example II for Western blotting and incubated with one of the following primary antibodies: mouse monoclonal anti-eGFP (B2) (Santa Cruz Biotechnology; sc-9996) (1:1000), rabbit polyclonal anti-FLAG (Sigma-Aldrich; F-7425) (1:3000), rabbit polyclonal anti-hDIS3L (Sigma-Aldrich; HPA041805, lot: R38591) (1:500), rabbit polyclonal anti-hRRP6 (Sigma-Aldrich; P4124) (1:3000), rabbit polyclonal anti-hDIS3L2 (home-made; see Lubas et al. 2013) (1:2000), goat polyclonal anti-GAPDH (V-18) (Santa Cruz Biotechnology; sc-20357) (1:2000). Membranes were then washed with TBST and incubated with appropriate secondary antibody (goat anti-mouse, goat anti-rabbit (Calbiochem; 401215, 401393, respectively) or rabbit anti-goat (Sigma-Aldrich; A5420)) conjugated with horseradish peroxidase. Eventually, blots were developed in Curix 60 machine (AGFA), using Immun-Star™ WesternC™ Kit (Bio-Rad) and CL-XPosure™ Films (Thermo Scientific).
Immunolocalization Analysis.
2×104 cells of HEK293 Flp-In T-Rex-derived stable cell lines were plated onto chamber slides (Thermo Scientific), in a medium with doxycycline (100 ng/ml) to induce protein expression. Cells were fixed permeabilized and blocked. Cells were incubated, first with primary antibody and then with secondary antibody coupled to fluorophore. Finally, cells were stained with DAPI (Invitrogen) After the final wash coverslips were mounted on chamber slides in ProLong Gold antifade reagent (Invitrogen), left in the dark overnight at 25° C. and then stored at 4° C. until microscopic analysis. The following antibodies were used (dilutions in parentheses): 1) primary-mouse monoclonal anti-FLAG (M2) (Sigma-Aldrich; F3165) (1:200); rabbit polyclonal anti-hDIS3 (Sigma-Aldrich; HPA039281, lot: R37348) (1:100); rabbit polyclonal anti-fibrillarin (Abcam; ab5821) (1:150); 2) secondary—Alexa Fluor 635-conjugated goat anti-mouse, Alexa Fluor 555-conjugated goat anti-rabbit IgG, Alexa Fluor (both from Molecular Probes) (1:800). Imaging was performed on a FluoView FV1000 system with spectral detectors (Olympus), using appropriate emission filters and a 60×/1.40 oil immersion objective lens. Images were processed using the FluoView software.
Polysome Gradients and RNA Isolation.
Stable cell lines were grown overnight in a medium containing doxycycline on one plate until reaching ˜95% confluence. Cells were treated with cycloheximide (Sigma-Aldrich; 200 μg/ml) at 37° C. for 15 minutes, harvested by trypsinization, spun down for 1 minute at 500 xg; 4° C. and then washed 3 times with ice-cold PBS containing 100 μg/ml cycloheximide. After final wash and complete removal of PBS, the cells were suspended in 0.5 ml of lysis buffer (10 mM Hepes-KOH, pH=7.5; 100 mM KCl; 2.5 mM MgCl2; 1 mM DTT; 100 μg/ml cycloheximide; 1 mg/ml heparin (Sigma-Aldrich); 1% reduced Igepal-CA630 (Sigma-Aldrich); 80 u/μl RiboLock™ RNase Inhibitor (Thermo Scientific); 1 x Protease Inhibitor Cocktail, Complete EDTA-free (Roche)), lysed by thorough pipetting and incubation for 15 minutes at 4° C. on a rotating wheel. Lysates were then centrifuged for 10 minutes at 10000 xg; 4° C. and RNA concentration in collected supernatants was measured using Nanodrop 2000 c device (Thermo Scientific). 8 OD260 units of cytoplasmic lysates in 500 μl of lysis buffer were layered onto 7-47% sucrose gradients (prepared using filtered sucrose solutions in lysis buffer, lacking detergent and ribonuclease inhibitor) and ultracentrifuged for 2 hours at 39000 xg; 4° C. in SW-41 Ti rotor (Beckman Coulter). Subsequently, 0.5 ml fractions were collected from each gradient by pumping 60% sucrose solution (prepared as above) to the bottom of tubes and OD260 was monitored on ÄKTA Purifier. For RNA isolation from polysome gradients, collected fractions were thoroughly mixed with 650 μl of phenol: guanidine thiocyanate (1:1) by vortexing and incubated at 65° C. for 8 minutes. Then, 320 μl of chloroform and 120 μl of 3M sodium acetate (pH=5.2) were added, followed by vortexing and centrifugation for 5 minutes at 13200 rpm. Samples were extracted twice with chloroform and RNA was precipitated from aqueous phase with isopropanol, washed with 75% ethanol and suspended in 20 μl of RNase-free water. RNA samples were subsequently pooled and analysed by northern-blotting.
siRNA Transfection.
siRNA-mediated knockdown was done using stealthRNA and Lipofectamine RNAiMAX (Invitrogen). The stealthRNA oligo against hRRP6 (ID HSS182420) or negative control (Stealth™ RNAi Negative Control Low GC) (both from Invitrogen) were used at the final concentration of 20 nM. The cells were grown in the absence or presence of doxycycline for additional 60 hours before harvesting.
Reverse Transcription and Quantitative PCR.
10 μg of total RNA extracted from stable human cell lines were treated with 6 units of TURBO™ DNase (Ambion), in the presence of RiboLock™ RNase Inhibitor (Thermo Scientific), Following extraction, 2 μg of DNase-treated RNA was reverse transcribed by using a mixture of 50 pmol of an oligo(dT) primer and 250 ng of random hexamers (Invitrogen) and Superscript III™ reverse transcriptase (Invitrogen) 1/100 of a cDNA reaction was mixed with Platinum® Quantitative PCR SuperMix-UDG (Invitrogen), 2.5 pmol of each primer and 0.3 μg BSA in triplicates, in a final volume of 10 μl and applied to real-time PCR analysis in a Roche LightCycler® 480 system using an annealing temperature of 58° C. Negative controls lacking reverse transcriptase showed a negligible background. All data were normalized to GAPDH mRNA. The following specified in Table 5 primer pairs were used for amplification.
Since the results of biochemical analyses and experiments performed using yeast cells were quite promising, it prompted to test the impact of hDIS3 mutations in humans using a proper cellular model. The most appropriate would be obviously multiple myeloma cell lines bearing different hDIS3 variants. However, sequence analysis that were performed for several commercially available MM cell lines from patients did not reveal presence of mutations in hDIS3 gene (data not shown). On the other hand, although I845V mutation was present in SKMM1 cell line (data not shown), it was not advantageous to use this line since the mutation does not influence the exoribonucleolytic activity of the enzyme (
With the use of the model cell lines, it was examined whether expression of mutated versions of hDIS3 protein leads to aberrations in metabolism of RNA molecules representing various classes. To this end, total RNA from the cell lines was isolated, which cell lines were either untreated or subjected to doxycycline-mediated induction for 48 hours. It is important to note that following such period of induction, cell lines expressing hDIS3D487N and hDIS3R780K (mutant proteins grew slightly worse than those producing hDIS3WT or hDIS3G766R variants.
First, low-resolution northern-blot analysis was applied to selected transcripts synthesized by different RNA polymerases. Above all, massive accumulation of unprocessed 5.8S ribosomal RNA precursors (RNA polymerase I transcripts) was noticed following doxycycline-mediated induction in cell lines bearing mutated hDIS3 variants (but not wild-type counterpart) (
As the degree of accumulation was the strongest for 5.8S rRNA precursors, it was analyzed in more detail by high-resolution northern blots, using 5S rRNA as loading control (
Two recent publications underscored importance of exosome and catalytic Dis3 activities in particular, in the global degradation of non-coding RNAs in yeast (Schneider et al. 2012; Gudipati et al. 2012). In both high-throughput analyses, accumulation of tRNAs and their precursors was reported as one of the most prominent phenotypes resulting from Dis3 inactivation. This, together with the finding that levels of some other RNA polymerase III transcripts are increased in cells producing mutant variants of hDIS3 (
The impact of hDIS3 mutations on the accumulation of U3 snoRNA and three different snRNAs was also investigated, however only for U5 snRNA the phenotype resembling the one observed previously in the case of tRNAs was noticed (
In summary, hDIS3 mutations in the model cell lines resulted in accumulation of majority of analyzed exosome substrates, including 5.8S processing intermediates, tRNAs, RNA polymerase III transcripts and PROMPTs. Although the degree of accumulation was variable between different exosome targets, it was clearly observed that in most of the cases it was more significant in the cells producing hDIS3 D487N and hDIS3R780K than hDIS3G766R variant.
MM-Associated hDIS3 Mutations Lead to Growth Inhibition of Human Model Cell Lines.
Cell Growth Analyses.
Stable inducible HEK293 Flp-In T-REx cell lines obtained in Example III were grown as described in Example III until reaching 90% confluence. In the 48-hours induction mode 2×105 cells were plated directly on plates in a medium either lacking or containing doxycycline and grown for another 48 hours. In the 48-hours +48-hours induction mode the cells were first re-plated onto new plates, in a medium either lacking or containing doxycycline, at 40% confluence; 48 hours later, 2×105 cells were plated on plates in the respective medium and grown for another 48 hours. Cell growth and eGFP fluorescence were analyzed using Olympus IX81 microscope (Olympus)
“Cell competition assay” was carried out by mixing equal amounts (5×105) of “empty” HEK293 Flp-In T-REx and established stable cell line, plating them on plates, followed by induction of expression with doxycycline. Eventually, microscopy observations were performed as described above.
Assays on metabolic activity of the cell lines were performed as follows. 5000 of the cells were plated in triplicates (for each cell line, condition and time of measurement) on 96-well plates. Cells were either maintained in a medium lacking doxycycline or subjected to treatment with the inducer. 24 hours, 72 hours or 120 hours following induction, 10 μl of AlamarBlue® (Invitrogen) was added to the cultures. Amounts of the reduced reagent were quantified after 120 minutes using DTX880 Multimode Detector (Beckman Coulter). During the growth of cells for RNA isolation, it was noticed that cell lines expressing hDIS3 variants with D487N or R780K substitutions grew slightly worse following 48-hour-long period of doxycycline-mediated induction than two other cell lines. To be absolutely sure whether hDIS3 mutations have impact on cell physiology, additional growth analysis for established cell lines was performed through the observation of the uninduced cells and their counterparts subjected to a prolonged induction (that is: 48 hours, followed by passage of the cells into the fresh medium containing doxycycline and another 48 hours of culture) using a fluorescence microscope. While all of them grew normally and comparably in a medium lacking doxycycline (data not shown), upon longer-term sh-miRNA-mediated repression of endogenous hDIS3 and simultaneous expression of sh-miRNA-insensitive exogenous hDIS3 variants, the four cell lines behaved differently (
It is worth noting that virtually all cells in the field of view displayed eGFP fluorescence (
More detailed comparative analysis of cell viability was performed for the model cell lines using AlamarBlue® reagent. The assay basing on this compound relies on the fact that it is reduced by metabolically-active cells upon addition to cell culture, which changes its spectral properties. The amount of reduced form of the compound reflects the metabolic activity of the cells and—indirectly—their viability (resultant of both proliferation and mortality). Therefore colorimetric measurements of the amounts of reduced AlamarBlue® in cell lines with transgenes coding for different hDIS3 variants were carried out, the cells being either uninduced or subjected to doxycycline treatment. It was noticed that the results were strikingly similar for all cell lines, provided that the co-expression of sh-miRNA and exogenous hDIS3 was not induced (
Summing up, with the use of the experimental system it was demonstrated that hDIS3 D487N catalytic mutation, as well as G766R and R780K substitutions found in multiple myeloma patients, lead to the enhanced mortality and inhibition of metabolic activity of human cells. Growth defects might be an ultimate result of aberrations in RNA metabolism.
MM-Associated Mutations in hDIS3 RNB Domain are Synthetically Lethal with PIN Domain Catalytic Mutation in Human Cells.
Oligonucleotides, Plasmids and Cloning.
pMM11, pMM12, PMM13, pMM14 constructs were generated by site-directed mutagenesis using D3PINF (AGGAATAACCGGGCGATTCGAGTAGCAGCAAAATGGTACAATG) (SEQ ID NO: 97)- D3PINR (TCGAATCGCCCGGTTATTCCTGTCATTAGCATTT TCTCCCTG) (SEQ ID NO: 98)-oligonucleotide pair and pMM7, pMM8, pMM9, pMM10 plasmids constructed in example III as respective templates. These construct were generated in order to examine influence of mutation occurring in PIN and RNB domains simultaneously.
Cell Culture, Generation of Stable Cell Lines and Cell Growth Analyses.
Cell lines were obtained as described in Example III but with the use of pMM11, pMM12, pMM13, pMM14 constructs. Cells were grown in conditions described in Example III. Cell growth was analyzed as described in Example IV.
RNA Isolation and Northern-Blot Analysis.
RNA isolation and high-resolution northern-blots were performed as described in Example III.
Reverse Transcription and Quantitative PCR.
Reverse transcription and quantitative PCR were performed as described in Example III.
Next, it was important to evaluate whether there might be any effect of hDIS3 PIN domain endonuclease activity in the background of different hDIS3 RNB domain mutations. To this end, analogous stable cell lines were constructed, bearing additional mutation in the catalytic site of PIN domain—D146N—which was previously shown to abolish hDIS3 endonucleolytic activity in the in vitro assays. Cell growth assays performed as described in Example IV, revealed that the growth of cell lines expressing different hDIS3 versions with D146N mutation was rather unaffected in the absence of doxycycline (
Then, an analysis of molecular phenotypes resulting from combination of MM-associated hDIS3 mutations within RNB domain with disruption of PIN domain endonucleolytic activity was carried out by comparing the cells to respective single mutants. As shown in the
2-hydroxy-(4H)-isoquinoline-1,3-dione Inhibits the Endonucleolytic Activity of the Isolated PIN domain of hDIS3 in vitro.
2-hydroxy-(4H)-isoquinoline-1,3-dione Synthesis.
2-hydroxy-(4H)-isoquinoline-1,3-dione synthesis was performed according to method described in Bioorganic & Medicinal Chemistry vol. 20 ((2012) 467-479).
Mixture of homophtalic anhydride 1 (0.5 g; 3.08 mmol), benzylhydroxylamine hydrochloride (0.59 g; 3.7 mmol) and triethylamine (0.5 ml; 3.7 mmol) in toluene was heated on heating mantle with Dean-Stark apparatus for 4 h. Substrate is still visible on LCMS, adding amines and heating nothing change so reaction was stopped. White solid was removed by filtration.
The filtrate was concentrated to dryness, dissolved in DCM and purified by column chromatography AcOEt/hexane ⅛. Crystallization from Et2O gave 0.39 g (48%) of 2 as white solid. 1H NMR (CDCl3, 200 MHz):
δ8.23 (d, 2H, J=9Hz); 7.66-7.26 (m, 8H); 5.15 (s, 2H); 4.15 (s, 2H).
To a solution of 2 (0.36 g; 1.34 mmol) in DCM, BBr3 (0.25 ml; 2.66 mmol) was added in one portion and reaction was stirred for 1 h at rt. Then reaction was carefully quenched with water. After 15 min yellow solid was removed by filtration. The filtrate was extracted with DCM, dried over MgSO4 and concentrated. Crystallization from Et2O gave 0.088 g of 3 as beige solid. 1H NMR (DMSO, 200 MHz): δ10.39 (brs, 1H); 8.01 (d, 1H, J=8Hz); 7.68-7.36 (m, 3H); 4.25 (s, 2H).
Activity Assay.
Recombinant protein was obtained with the method described in Example I. Substrate for in vitro biochemical assay was a 5′ fluoresceine-labeled ss17-(A)34, which was prepared similarly to substrates described in Example I. The activity assay was performed as described in Example I in the presence or absence of 2-hydroxy-(4H)-isoquinoline-1,3-dione.
After establishing that inactivation of the hDIS3 PIN domain is synthetically lethal with MM associated mutations in the RNB domain, a search for for putative inhibitors was conducted. 2-hydroxy-(4H)-isoquinoline-1,3-dione, which is a well-known inhibitor of RNAse H (Hang et al. 2004) was synthetized. In order to conduct an activity assay for human DIS3 PIN domain endonucleolytic activity, a recombinant version of isolated PIN domain (
2-hydroxy-(4H)-isoquinoline-1,3-dione Specifically Inhibits Growth of Yeast Strains Harboring mutations in yeast DIS3 gene corresponding to those found in MM patients.
Yeast Growth Assays.
Yeast strains harboring G833R or R847K mutations, obtained in Example II were used for growth assays. Serial dilutions of indicated yeast strains were spotted on YPD plates and YPD plates with 2-hydroxy-(4H)-isoquinoline-1,3-dione (at 1 mM concentration) and incubated at 30° C. for 60 hours.
Knowing that 2-hydroxy-(4H)-isoquinoline-1,3-dione inhibits DIS3 PIN domain activity, an in vivo analysis was performed using yeast strains harboring G833R or R847K mutations, obtained in Example II (
In the below Table 6 there are gathered hDIS3 RNB domain mutations (the position of which relates to SEQ ID NO: 1) and theirs equivalents in yeasts or cell lines.
By the above Examples it has been confirmed that in vivo that application of the compound which inhibits hDIS3 PIN domain activity lead to growth inhibition of the cells having disturbances in hDIS3 activity, especially mutations in hRNB Dis3 domain. These confirms that the compounds which are inhibitors of hDIS3 PIN domain are also effective therapeutic agents for treatment of cancers in which there are mutations in hDIS3 RNB domain. Cancers in which there are mutations in hDIS3 RNB domain can be selected form multiple myeloma, medulloblastoma and acute myeloid leukaemia. The mutations in the RNB domain of the hDIS3 are preferably selected from mutation S477R, G766R, R780K, V504G, A524P, I845V with relation to SEQ ID NO: 1.
| Number | Date | Country | Kind |
|---|---|---|---|
| P.403980 | May 2013 | PL | national |
The present application is filed pursuant to 35 U.S.C.§371 as an U.S. National Phase Application of International Patent Application No. PCT/IB2014/061558 filed on May 20, 2104, which published as PCT Publication No. WO 2014/188337 on Nov. 27, 2014, which claims benefit of Polish Patent Application Number P.403980 filed May 21, 2013 and U.S. Provisional Application No. 61/825,739 filed May 21, 2013.
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/IB2014/061558 | 5/20/2014 | WO | 00 |
| Publishing Document | Publishing Date | Country | Kind |
|---|---|---|---|
| WO2014/188337 | 11/27/2014 | WO | A |
| Number | Name | Date | Kind |
|---|---|---|---|
| 20020064784 | Friend | May 2002 | A1 |
| Entry |
|---|
| Chapman et al. Nature Mar. 2011 467-472. |
| Hiroaki et al. PLoS ONE Mar. 2007 e317 1-12. |
| International Preliminary Report on Patentability dated May 22, 2015, which issued during prosecution of International Application No. PCT/IB2014/061558. |
| International Search Report dated Sep. 24, 2014, which issued during prosecution of International Application No. PCT/IB2014/061558. |
| Chapman, et al. “Initial genome sequencing and analysis of multiple myeloma” Nature, Mar. 2011, 471(7339):467-472. |
| Murakami, et al. “Ribonuclease Activity of Dis3 is Required for Mitotic Progression and Provides a Possible Link between Heterochromatin and Kinetochore Function” PLoS ONE, Mar. 2007, 2(3):e317. |
| Papandreou, et al. “Identification of an Ire1alpha endonuclease specific inhibitor with cytotoxic activity against human multiple myeloma” Blood, Jan. 2011, 117(4):1311-1314. |
| Schaeffer, et al. “The exosome contains domains with specific endoribonuclease, exoribonuclease and cytoplasmic mRNA decay activities” Nature Structural & Molecular Biology, Jan. 2009, 16(1):56-62. |
| Sun, et al. “Catalytic mechanism of Escherichia coli ribonuclease III: kinetic and inhibitor evidence for the involvement of two magnesium ions in RNA phosphodiester hydrolysis” Nucleic Acids Research, Feb. 2005, 33(3):807-815. |
| Number | Date | Country | |
|---|---|---|---|
| 20160090639 A1 | Mar 2016 | US |
| Number | Date | Country | |
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| 61825739 | May 2013 | US |
