Patent Application
20140096284
Studying the role of the components found in living cells is one of the major purposes of basic biology research. These components are biomolecules or chemical products that have a particular function of interest in the cell. A traditional way of studying the function of such molecules has been the manipulation of the genome, by the over-expression or silencing of nucleic acid sequences like genes or regulatory sequences related to the molecule of interest.
In recent years, delivery of molecules other than nucleic acids has been a major focus of study, mostly in biomedical sciences, where different drug delivery systems have been developed (Ravichandran, 2009). The intracellular delivery of chemicals (e.g., hormones, dyes or inhibitors), proteins (e.g., antibodies, labeled proteins or enzymes) and other biomolecules or a combination of molecules has an enormous potential for basic and applied research. The direct intracellular delivery of a protein could, for instance, save the time, cost and labor needed to create a transgenic organism that expresses it. Moreover, the protein could be labeled for monitoring or have different modifications that cannot be achieved by its expression in the host organism. Also, the functional analysis of the protein could be multidimensional if it could be co-delivered with other biomolecules or chemicals related to its role in the cell. For biotechnological purposes, the delivery of enzymes with a specific function for gene editing or DNA recombination could for instance improve plant transformation efficiency or facilitate genome edition.
Several technology platforms have been developed to achieve intracellular protein delivery, like cell penetrating peptides or protein transduction domains (Eggenberger et al., 2009). These short peptides, after incubation with the molecule of interest, are able to cross the cell membrane and deliver the cargo (Eggenberger et al., 2009). This technology has been effective to deliver biomolecules to plant tissues overcoming the plant cell wall (Chugh et al., 2009; Lu et al., 2010; Eggenberger et al., 2011; Qi et al., 2011). However, this technique requires peptide synthesis, incubation parameters optimization, and generally a covalent bond to a protein of interest (Lu et al., 2010), and the uptake could depend on the type of plant tissues (Qi et al., 2011).
Another technology platform that is nowadays a major focus of research is nanoparticle mediated drug delivery (Ravichandran, 2009). Most of the research in this area is in the biomedical field, where nanoparticles are being tailored to deliver a particular molecule to a specific cell under certain conditions. Nevertheless, nanoparticle mediated molecule delivery to plant cells has been reported, including delivery of nucleic acids (Vijayakumar et al., 2010; Wang et al., 2011; Martin-Ortigosa et al., 2012a; Martin-Ortigosa et al., 2012b; Naqvi et al., 2012), proteins (Martin-Ortigosa et al., 2012a), and chemicals (Grichko et al., 2006; Torney et al., 2007; Wild and Jones, 2009). The suitability of mesoporous silica nanoparticles for chemical and DNA (Torney et al., 2007), or protein and DNA co-delivery (Martin-Ortigosa et al., 2012a) through the biolistic method, has also been reported. The use of nanoparticles for delivery of substances to plant cells is a technology currently in development and it can require nanoparticle modifications such as increasing nanoparticle density for a better biolistic delivery to plant tissues (Martin-Ortigosa et al., 2012b), or increasing the pore size for protein loading (Martin-Ortigosa et al., 2012a).
In order to improve plant genetic transformation, Wu and colleagues followed two biolistic approaches to deliver transposomes using 1 μm gold microparticles (Wu et al., 2011a; Wu et al., 2011b). In both cases, the surface of the gold microparticle had to be modified to promote the binding of helper proteins that locked the transposome to the projectile and favored the release of that complex once it was intracellularly delivered. Also, the transposon sequence or the transposase itself had to be engineered to form this microparticle-protein-DNA complex (Wu et al., 2011a; Wu et al., 2011b).
More than a decade ago the term “diolistics” was used for the first time to refer to a technique in which dyes were dried onto microprojectiles and bombarded to nervous system cells for labeling (Gan et al., 2000). This technique has been used to deliver dyes indicators of cellular physiological state or permitted the visualization of cell architecture (O'Brien and Lummis, 2007; Roizenblatt et al., 2006). This method has also been used in plant and algal cells to monitor changes in cytosolic calcium concentrations (Bothwell et al., 2006). In this technique, a dye (chemical compound) is solubilized in a solvent like water (Roizenblatt et al., 2006) or methylene chloride (Gan et al., 2000). This solution is mixed with a particle suspension (gold or tungsten particles of 0.6-1.7 μm in diameter). This mix is usually poured over a glass slide and left to dry by evaporation. While the solvent evaporates, the dye is precipitated over the surface of the particles. The dye coated projectiles are then used for bombardment of different tissues. Delivery of RNA to parasitic helminths has also been achieved by the biolistic method, lyophilizing the RNA onto 1.6 μm gold microcarriers (Davis et al., 1999).
The present invention provides for the coating of particles, such as microparticles or nanoparticles (collectively “particles”), e.g., those formed of gold, tungsten, mesoporous silicate, silver, quantum dots, carbon nanotubes, or polystyrene beads, with molecules including molecules of biological origin (biomolecules) and chemicals (agents that are not obtained from cells or viruses) using lyophilization (“freeze-drying”) or air-drying (drying that does not rely on sublimation or the use of temperatures below 0° C.; rather it relies on evaporation at temperatures above 0° C., e.g., around 4° C. to about 28° C.). The freeze-dry method uses sublimation to precipitate the molecules over projectiles, thereby preserving the integrity and activity of molecules such as biomolecules that could be degraded or are more sensitive to degradation using simple evaporation methods.
In one embodiment, the present invention employs the biolistic method to deliver, for example, bioactive enzymes, to plant cells using a projectile coating that is achieved by lyophilizing (freeze-drying) or air-drying a solution of proteins along with a projectile suspension. Lyophilization is fast, and prevents macromolecules such as proteins from degradation. Delivery of lyophilized molecules to cells may be useful for transient modifications, e.g., using proteins with a short half-life, or in gene editing, where the delivery of an enzyme involved in DNA recombination, DNA cleavage, or DNA modification could help to engineer accurately plant genomes including organelle genomes such as chloroplasts and mitochondria, thereby improving plant transformation or allowing for precise gene edition. Since the gene gun is also applied in medical sciences for therapeutic purposes, the bombardment of organisms such as humans, or a canine, equine, bovine, swine, caprine, ovine, feline, or non-human primates with lyophilized agents, e.g., proteins or peptides, could deliver vaccines and other therapeutic substances.
As disclosed below, DNA encoding protein or proteins were freeze-dried or air-dried onto the surface of particles and those coated particles were successfully delivered to plant cells and mouse cells after bombardment and the proteins were shown to be active. The present method offers a straight forward, simple and inexpensive way of achieving delivery of any molecule, including proteins or other molecules, and including combinations of molecules, to cells such as plant cells and mammalian cells. The present freeze-dry or air-dry method does not require loading of particle pores with molecules, the use of particle surface modifying moieties, e.g., to covalently link agents to the surface, or techniques for efficient release of the agent once the particles reach the cell.
In one embodiment, solutions of molecules such as proteins, hormones, or enzymes are freeze-dried or air-dried over particles, allowing for layering over the surface of the particles. For instance, a mixture of a solution having one or more isolated molecules and a particle suspension is layered over a macrocarrier (which is also referred to as a cartridge or projectile holder) used for bombardment. This loaded macrocarrier is frozen in liquid nitrogen for several minutes and then freeze-dried in a lyophilizer, or is subjected to air-drying. The methodology works not only with proteins, but also with chemicals and combinations of molecules. The freeze-dry or air-dry coating method thus provides for delivery of molecules such as proteins, or mixtures of molecules, such as plasmid DNA and proteins.
As disclosed herein, particles are not required to deliver molecules using biolistic methods. In one embodiment, a mixture of a solution having one or more molecules is layered over a macrocarrier or other substrate, which is subjected to freeze drying or air drying, and then loaded in the gene gun. Thus, in one embodiment, solutions of molecules such as proteins or nucleic acid are freeze-dried or air-dried, thereby producing a dried materials, which is then layered over a macrocarrier used for bombardment. In another embodiment, a solution of molecules, e.g., liquid droplets having molecules, are placed on over a macrocarrier used for bombardment.
In one embodiment of the invention, the molecule, or particle and molecule containing mixture, can be in cell media, ethanol, water or a buffer, e.g., phosphate buffered saline, prior to freeze-drying or air-drying. Any suitable and effective solvent can be employed.
In one embodiment, the freeze-dry or air-dry process is employed directly with a macrocarrier that is going to be used in bombardment. In another embodiment, the freeze-dry or air-dry process is employed with a solution of particles and at least one agent in a tube, slide or other receptacle, where the coated particles are then loaded in the gene gun.
In one embodiment, the invention provides a plurality of particles such as tungsten or gold particles, e.g., about 0.3 μm to about 3.0 μm in diameter or about 0.2 μm to about 1.2 μm in diameter, having a freeze-dried or air-dried coating of at least one isolated molecule. The molecule may be, for example, isolated protein, isolated hormone, isolated glycoprotein, isolated nucleic acid, e.g., isolated DNA, or a mixture or complex of isolated nucleic acid and isolated protein.
Also provided is a method to prepare particles coated with at least one molecule. The method includes providing a substrate having a solution with a mixture of a plurality of particles, e.g., of about 0.3 μm to about 1.2 μm in diameter, and at least one isolated molecule, and freeze-drying or air-drying the solution in or on the substrate to provide a preparation of particles coated with the at least one molecule. In one embodiment, the molecule is not isolated nucleic acid such as isolated ribonucleic acid. In one embodiment, the at least one molecule is an enzyme.
Further provided is a method to deliver at least one molecule to a eukaryotic cell, such as a plant cell or a mammalian cell. The method includes providing a plurality of particles, for instance, gold particles of about 0.2 μm to about 2 μm in diameter, having a freeze-dried or air-dried coating with at least one isolated molecule; and biolistically delivering the plurality to eukaryotic cells such as mammalian cells in an amount effective to deliver the at least one molecule into the cells. The method also includes providing freeze-dried or air-dried isolated molecules, or a combination of isolated molecules, for instance, about 0.2 μm to about 3 μm in diameter; and biolistically delivering the freeze-dried or air-dried isolated molecules (i.e., in the absence of particles) to eukaryotic cells in an amount effective to deliver the at least one molecule into the cell. In one embodiment, the cells are in a plant. In one embodiment, the cells are mammalian cells in a mammal. In one embodiment, the coated particles, or freeze-dried or air-dried material, may be delivered to wounds, skin, tumor cells, mucosal tissue, retina and the like. In one embodiment, the particles are on a macrocarrier substrate. In one embodiment, the molecule comprises isolated nucleic acid, enzyme, antibacterial agent, antifungal agent, antiviral agent, or hormone. In one embodiment, the molecule is an antigen and so may be useful to vaccinate an animal. In one embodiment, the molecule is an enzyme and so may be useful for enzyme replacement therapy. In one embodiment, the molecule is a drug including biologics and so may be useful for other therapies including cancer therapy and wound healing.
In one embodiment, the invention provides a method to treat an animal having a disorder. The method includes providing an amount of a plurality of particles having a freeze-dried or air-dried coating with at least one molecule, wherein an amount of the molecule when administered to an animal is effective to inhibit or prevent at least one symptom associated with the disorder. The amount is biolistically delivered to an animal having the disorder. In one embodiment, the animal is a mammal, e.g., a human. In one embodiment, the disorder is cancer. In one embodiment, the amount is delivered to the epidermis of the animal.
If particles are employed in biolistic transformation, those particles may be formed of any material, including but not limited to gold, tungsten, silica, nickel, silver, platinum, palladium, titanium, iron, alumina, copper, metal alloys, calcium phosphate, emulsified wax, ceramics, carbon, chitosan, cellulose, lignine, chitin, starch, alginate, hyaluronan, dextran, cyclodextrins, dextran, arabinogalactan, pullulan, heparin, polystyrene, styrene, poly(vinylpyridine), polyvinyl alcohol, titanium oxide, cerium oxide, cadmium selenide or zinc sulfide, and may have a shape including but not limited to a sphere, a rod, a whisker, a cylinder, a tube, a cone, a prism, a polyhedron, with diameters or dimensions including from but not limited to, for a sphere 4 nm to 3 μm, for a rod 10×30 nm to 50×200 nm, for a whisker 5 nm to 5 μm, for a cylinder 5 nm to 5 μm, for a tube 5 nm to 5 μm, for a cone 4 nm to 3 μm, for a prism 4 nm to 3 μm, and for a polyhedron from 4 nm to 3 μm.
The term “amino acid,” comprises the residues of the natural amino acids (e.g., Ala, Arg, Asn, Asp, Cys, Glu, Gln, Gly, His, Hyl, Hyp, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, and Val) in D or L form, as well as unnatural amino acids (e.g., phosphoserine, phosphothreonine, phosphotyrosine, hydroxyproline, gamma-carboxyglutamate; hippuric acid, octahydroindole-2-carboxylic acid, statine, 1,2,3,4,-tetrahydroisoquinoline-3-carboxylic acid, penicillamine, ornithine, citruline, α-methyl-alanine, para-benzoylphenylalanine, phenylglycine, propargylglycine, sarcosine, and tert-butylglycine). The term also comprises natural and unnatural amino acids bearing a conventional amino protecting group (e.g., acetyl or benzyloxycarbonyl), as well as natural and unnatural amino acids protected at the carboxy terminus (e.g., as a (C1-C6)alkyl, phenyl or benzyl ester or amide). Other suitable amino and carboxy protecting groups are known to those skilled in the art (See for example, T. W. Greene, Protecting Groups In Organic Synthesis; Wiley: New York, 1981, and references cited therein).
The term “polypeptide” describes a sequence of at least 50 amino acids (e.g., as defined hereinabove) or peptidyl residues while a peptide describes a sequence of at least 2 and up to 50 amino acid residues. The sequence may be linear or cyclic. For example, a cyclic peptide can be prepared or may result from the formation of disulfide bridges between two cysteine residues in a sequence. A polypeptide can be linked to other molecules through the carboxy terminus, the amino terminus, or through any other convenient point of attachment, such as, for example, through the sulfur of a cysteine. In one embodiment of the invention a polypeptide comprises about 50 to about 300 amino acids. In another embodiment a peptide has about 5 to about 25 amino acids Peptide and polypeptide derivatives can be prepared as disclosed in U.S. Pat. Nos. 4,612,302; 4,853,371; and 4,684,620, or as described in the Examples hereinbelow. Polypeptide sequences specifically recited herein are written with the amino terminus on the left and the carboxy terminus on the right.
The term “nucleic acid”, “polynucleic acid” or “polynucleic acid segment” refers to deoxyribonucleotides or ribonucleotides and polymers thereof in either single- or double-stranded form, composed of monomers (nucleotides) containing a sugar, phosphate and a base which is either a purine or pyrimidine. Unless specifically limited, the term encompasses nucleic acids containing known analogs of natural nucleotides which have similar binding properties as the reference nucleic acid and are metabolized in a manner similar to naturally occurring nucleotides. Unless otherwise indicated, a particular nucleic acid sequence also implicitly encompasses conservatively modified variants thereof (e.g., degenerate codon substitutions) and complementary sequences as well as the sequence explicitly indicated. Specifically, degenerate codon substitutions may be achieved by generating sequences in which the third position of one or more selected (or all) codons is substituted with mixed-base and/or deoxyinosine residues (Batzer et al., 1991; Ohtsuka et al., 1985; Rossolini et al., 1994). An “oligonucleotide” typically includes 30 or fewer nucleotides.
As used herein, the terms “isolated and/or purified” refer to in vitro preparation, isolation and/or purification of a nucleic acid or protein (polypeptide or peptide) so that it is not associated with in vivo substances, or is substantially purified from in vitro substances. Thus, with respect to an “isolated nucleic acid molecule”, which includes a polynucleotide of genomic, cDNA, or synthetic origin or some combination thereof, or an “isolated polypeptide or peptide”, the “isolated nucleic acid molecule” or “isolated polypeptide or peptide” (1) is not associated with all or a portion of cell based molecules with which the “isolated nucleic acid molecule” or “isolated polypeptide or peptide” is found in nature, (2) is operably linked to a molecule which it is not linked to in nature, or (3) does not occur in nature as part of a larger sequence. An isolated nucleic acid molecule means a polymeric form of nucleotides of at least 10 bases in length, either ribonucleotides or deoxynucleotides or a modified form of either type of nucleotide. The term includes single and double stranded forms of DNA. The term “oligonucleotide” referred to herein includes naturally occurring, and modified nucleotides linked together by naturally occurring, and non-naturally occurring oligonucleotide linkages. Oligonucleotides are a polynucleotide subset with 200 bases or fewer in length. In one embodiment, oligonucleotides are 10 to 60 bases in length including 12, 13, 14, 15, 16, 17, 18, 19, or 20 to 40 bases in length. Oligonucleotides may be usually single or double stranded. Oligonucleotides can be either sense or antisense oligonucleotides. The term “naturally occurring nucleotides” referred to herein includes deoxyribonucleotides and ribonucleotides. The term “modified nucleotides” referred to herein includes nucleotides with modified or substituted sugar groups and the like. The term “oligonucleotide linkages” referred to herein includes oligonucleotides linkages such as phosphorothioate, phosphorodithioate, phosphoroselenoate, phosphorodiselenoate, phosphoroanilothioate, phosphoraniladate, phosphoroamidate, and the like.
The term “complexed” refers to binding of a molecule to a different molecule, typically through means other than covalent bonding. Such binding can take to form of, e.g., ionic or electrostatic interactions, or other attractive forces. For instance, DNA may be complexed with a protein.
Many molecules are unable to cross the membrane barrier of cells without the assistance of transport systems. For example, hydrophilic molecules are generally unable to cross the membrane barrier of cells without transport mechanisms due to the hydrophobic nature of the lipid bilayer, e.g., proteins are generally unable to cross the membrane barrier of cells without the assistance of protein transport systems. This challenge has led to the development of protein delivery systems using materials including polymers, carbon nanotubes and mesoporous silica nanoparticles. There are a few examples of protein delivery methodologies to cells such as plant cells, such as microinjection and cell penetrating peptides. While these methodologies could be used to introduce model proteins into plant cells, they have major disadvantages including the requirement of skillful handling of cell materials or lack of protection of the introduced protein during the process.
Delivery of bioactive, e.g., proteins, or co-delivery of bioactive agents, such as protein and DNA, to plant cells has great biological significance. Thus, with respect to delivery of protein and nucleic acid, in addition to the potential of enhancing genetic transformation and gene targeting in plants, researchers could assess loss or gain of function of different post-translationally modified forms of a protein, and protein interactions with other biomolecules. Also, direct delivery and release of proteins in plant cells could facilitate the understanding of cellular machinery or signal pathways more effectively. For example, this would allow for a greater understanding of protein functions in host cells where protein production pathways are impaired, or analyzing cellular regulatory functions through delivery of antibodies.
The biolistic method, or gene-gun method, is based on the bombardment of living organisms, tissues or cells using projectiles. Nanoparticle mediated delivery of bioactive (biogenic) molecules to plant cells, such as double or single stranded DNA and small interfering RNA, and delivery and release of chemical substances such as phenanthrene and plant growth regulators, have been reported. However, biolistic methods to deliver molecules in an effective amount to, for instance, intact plant cells, depend on the density of the delivery vehicle and the loading capacity of the vehicle.
The projectiles for plant biolistics usually are between 0.4 and 1.5 μm in diameter and are made of tungsten or gold. The biolistic method has been used mainly to deliver nucleic acid sequences like plasmid DNA to cells in order to create genetically modified organisms. Usually, these nucleic acids are physically precipitated on the projectile surface following a calcium chloride-spermidine based precipitation protocol (Klein et al., 1987; Sanford et al., 1993). This method is broadly used in plant transformation experiments. Plant researchers use homemade gene-guns or commercially available ones like PDS-1000/He from Bio-Rad. In recent years, other research fields like human and animal medicine are using gene-guns to deliver, for example, DNA vaccines (Haynes, 2004; de Andrés et al., 2009).
Table 1 summarizes delivery systems that are primarily used for plant transformation and includes the present method.
The present invention provides for freeze-dried or air-dried solutions of molecules, e.g., dyes, proteins and nucleic acids, coated onto particles, for biolistic delivery regardless of the presence or absence of a naturally occurring uptake or transport mechanism for the molecule, and may be used to deliver agents to any type of cell, including mammalian cells, e.g., ovine, porcine, equine, bovine, feline, canine or primates, such as humans, and plant cells.
The molecules for delivery include, but are not limited to, genes, nutrients (vitamins, etc.), and biocidal or pesticidal agents (e.g., insecticides or herbicides). For example, the term includes but is not limited to antibacterial agents, antifungal agents, antiviral agents, polypeptides, hormones, enzymes, antibodies, and RNA or DNA molecules of any suitable length, or any combination thereof. For instance, the RNA or DNA molecules may encode herbicide resistance, drought tolerance, a polypeptide associated with enhanced nutritional value, and the like.
Exemplary molecules for delivery to cells, including plant cells, include but are not limited to polypeptides, and/or polynucleotides (DNA or RNA) encoding a screenable marker, a polypeptide that can enhance or stimulate cell growth, an enzyme such as a recombinase, an integrase, a site-specific recombinase, a DNA topoisomerase, an endonuclease, a transposase, a restriction enzyme, a DNA polymerase, a DNA ligase, and the like, a transcription factor, a repressor, a DNA binding protein, a DNA repair protein a cell cycle protein, a RNA binding protein, RNase, a RNA-dependent RNA polymerase, ribosomal proteins, methyltransferase enzymes, hydroxylase enzymes, histone modifying enzymes, chromatin modifying enzymes, and the like.
In some examples the agent comprises a polynucleotide or polypeptide that stimulates cell growth. The agent employed in compositions for delivery to cells may provide a means for positive selection of recipient target cells, increased transformation efficiency, increased plastid transformation efficiency, increased gene targeting or combinations thereof. Genes that enhance or stimulate cell growth include genes involved in transcriptional regulation, homeotic gene regulation, stem cell maintenance and proliferation, cell cycle regulation, cell division, and/or cell differentiation.
The agent may be an antigen from any pathogen including any virus, bacteria, parasite or fungi that generates a pathological condition in an animal, e.g., one useful for a vaccine. The virus can be, for example, a herpesvirus, an influenza virus, a orthomyxovirus, a rhinovirus, a picornavirus, an adenovirus, a paramyxovirus, a coronavirus, a rhabdovirus, a togavirus, a flavivirus, a bunyavirus, a rubella virus, a reovirus, a measles virus, a hepadna virus, a filovirus, or a retrovirus (including a human immunodeficiency virus; including all clades of HIV-1 and HIV-2 and modifications thereof). The bacteria can be, for example, a mycobacterium (e.g., M. tuberculosis, which causes tuberculosis or M. leprae, which causes leprosy), a spirochete, a rickettsia, a chlamydia, or a mycoplasma. The parasite can be, for example, a parasite that causes malaria, and the fungus can be, for example, a yeast or mold. In one embodiment, the antigen is a glycoprotein. In one embodiment, the antigen is a viral capsid protein. In one embodiment, the antigen is a nonstructural protein, e.g., a protein that is not a viral polymerase. For example, the antigen may be any of the M, E or C proteins of West Nile virus, any of the N protein, P protein, M protein, F protein or glycoprotein of a paramyxovirus, or the capsid or a nonstructural protein of an astrovirus. In one embodiment, the antigen may be a toxin protein or a modified toxin protein, e.g., from Clostridium botulinum.
In one embodiment, the antigen may be a Meningococcus protein, a Streptococcus protein, a pneumococcus protein, a Neisseria protein or capsular polysaccharide, Hemophilus influenza protein, a Plasmodium falciparum protein, a mycobacterial protein, a protein from Bacillus anthracis, a protein from Corynebacerium diptheriae, a Bordetella pertussis protein, a protein or capsular polysaccharide from Salmonella typhi, a protein from Vibrio cholera, a HIV protein, a West Nile virus protein, a polio virus protein, a hepatitis B virus protein, a hepatitis C virus protein, an influenza virus protein, a respiratory syncytial virus protein or a dengue virus protein.
Pharmaceutical compositions of the present invention, suitable for inoculation comprise one or more agents such as antigens, e.g., isolated nucleic acid encoding one or more proteins thereof, optionally prepared (prior to freeze drying or air drying) using sterile aqueous or non-aqueous solutions, suspensions, and emulsions. The compositions can further comprise auxiliary agents or excipients, as known in the art. The composition of the invention is generally presented in the form of individual doses (unit doses). For instance, vaccines generally may contain about 0.1 to 200 mg, e.g., 30 to 100 μg, of protein into their composition.
When a composition of the present invention is used for administration to an individual, it can further comprise salts, buffers, adjuvants, or other substances which are desirable for improving the efficacy of the composition. For vaccines, adjuvants, substances which can augment a specific immune response, can be used. Normally, the adjuvant and the composition are mixed prior to presentation to the immune system, or presented separately, but into the same site of the organism being immunized.
A pharmaceutical composition according to the present invention may further or additionally comprise at least one chemotherapeutic compound, for example, for gene therapy, immunosuppressants, anti-inflammatory agents or immune enhancers, and for vaccines, chemotherapeutics including, but not limited to, gamma globulin, amantadine, guanidine, hydroxybenzimidazole, interferon-α, interferon-β, interferon-γ, tumor necrosis factor-alpha, thio semicarbarzones, methisazone, rifampin, ribavirin, a pyrimidine analog, a purine analog, foscarnet, phosphonoacetic acid, acyclovir, dideoxynucleosides, a protease inhibitor, or ganciclovir.
The administration of the composition (or the antisera that it elicits) may be for either a “prophylactic” or “therapeutic” purpose. In one embodiment, when provided prophylactically, the compositions of the invention which are vaccines are provided before any symptom or clinical sign of a pathogen infection becomes manifest. The prophylactic administration of the composition serves to prevent or attenuate any subsequent infection. In one embodiment, when provided prophylactically, a composition of the invention, is provided before any symptom or clinical sign of a disease becomes manifest. The prophylactic administration of the composition serves to prevent or attenuate one or more symptoms or clinical signs associated with the disease.
In one embodiment, when provided therapeutically, a vaccine is provided upon the detection of a symptom or clinical sign of actual infection by a pathogen. The therapeutic administration of the composition serves to attenuate any actual infection. In one embodiment, when provided therapeutically, a composition is provided upon the detection of a symptom or clinical sign of the disease. The therapeutic administration of the compound(s) serves to attenuate a symptom or clinical sign of that disease.
Thus, a vaccine composition of the present invention may be provided either before the onset of infection (so as to prevent or attenuate an anticipated infection) or after the initiation of an actual infection. Similarly, the composition may be provided before any symptom or clinical sign of a disorder or disease is manifested or after one or more symptoms are detected.
A composition is said to be “pharmacologically acceptable” if its administration can be tolerated by a recipient. Such an agent is said to be administered in a “therapeutically effective amount” if the amount administered is physiologically significant. A composition of the present invention is physiologically significant if its presence results in a detectable change in the physiology of a recipient, e.g., enhances at least one primary or secondary humoral or cellular immune response against at least one pathogen.
The “protection” provided need not be absolute, e.g., the infection need not be totally prevented or eradicated, if there is a statistically significant improvement compared with a control population. Protection may be limited to mitigating the severity or rapidity of onset of symptoms or clinical signs of the infection.
A typical regimen for preventing, suppressing, or treating a pathogen related pathology, comprises administration of an effective amount of a vaccine composition, administered as a single treatment, or repeated as enhancing or booster dosages, over a period up to and including between one week and about 24 months, or any range or value therein.
According to the present invention, an “effective amount” of a composition is one that is sufficient to achieve a desired effect. It is understood that the effective dosage may be dependent upon the species, age, sex, health, and weight of the recipient, kind of concurrent treatment, if any, frequency of treatment, and the nature of the effect wanted.
The invention will be further described by the following non-limiting examples.
200-800 cells were found to express GFP per sample, which is comparable to CaCl2/spermidine precipitation. TRITC-BSA was also successfully delivered to cells.
Onion epidermis tissue was obtained from the scale leaves of white onion bulbs. Rectangular (3×2.5 cm) pieces were peeled right before the bombardment and placed in solid agar media (0.5 mM of 2-(N-morpholino)ethanesulfonic acid (MES), 15 g L−1 Bacto agar (BD), pH 5.7) with the peeled side upwards. For quantitative measurements, epidermis pieces of the same scale leaf were distributed among treatments. Tobacco leaves (Nicotiana tabacum var. Petite Havana) were obtained from 3-6 week old in vitro grown plants on MS media (MS medium (Murashige and Skoog, 1962), 2% sucrose, 2.5 g L−1 gelrite, pH 5.7). Leaves were cut right before bombardment and put with the adaxial surface up on agar media. Hill maize immature embryos were obtained from immature ears provided by the Center for Plant Transformation—Iowa State University. Immature embryos (1-2 mm long) were cultured as previously described (Frame et al., 2000).
The saturated solutions of chemical or biomolecules used were done as follows (data per shot): for bromophenol blue (Sigma) delivery 10 μL of a 100 μg 1 μL−1 mg solution were used. For plasmid DNA delivery, 2 μL of a 500 ng μL−1 solution of the plasmid pLMNC95 (Luke Mankin and Thompson, 2001) were used for GFP expression. For mCherry expression linear dsDNA, the plasmid ER-rk (Nelson et al., 2007) was digested with the restriction enzymes SacI-HindIII. The digestion was electrophoresed in a 0.8% agarose gel and the 1885 bp band was cut and purified using a gel DNA extraction kit (IBI Scientific). For the delivery, 14 μL of a 2.8 ng μL−1 solution of the purified linear dsDNA were used. Plasmids pLMNC95 and ER-rk were obtained from the Arabidopsis Biological Resource Center. For maize embryo bombardment, 1 μg of plasmid pACH25 (Christensen and Quail, 1996) was used. For delivery of eGFP (Biovision) 2.5 μL of a 1 μg μL−1 were used. TRITC-BSA, β-glucuronidase and trypsin were dissolved in 250 mM NaCl, 15 mM Tris pH 8 buffer. For TRITC-BSA (Invitrogen), β-glucuronidase (Sigma), trypsin (Sigma) and RNAse A (Sigma) delivery, 10 μL of a 25 μg μL−1 solution, 5.5 μL of a 50 μg uL−1 solution, 4 μL of a 50 μg μL−1 solution and 4 μL of a 10 μg μL−1 solution respectively were used.
The solutions above described were mixed by pipetting with 2 μL of a 30 μg μL−1 solution in water of 0.6 μm gold microcarriers (Cat. 165-2262, Bio-Rad) per shot. The mix was loaded and evenly distributed over the inner circle (the central part that gets hit by the helium blast) of the macrocarrier set (a plastic macrocarrier already placed into the macrocarrier holder). The loaded macrocarrier set was frozen in liquid nitrogen for 5-10 minutes and freeze-dried using a lyophilizer (Freezone 2.5 from Labconco) for at least 1 hour. Bombardments were performed right after the lyophilization process.
For plant tissue bombardment the PDS-1000/He gene gun (Bio-Rad) was used according to the general settings described previously (Frame et al., 2000). All the gene gun supplies used are from Bio-Rad. For onion epidermis tissue, 1100 psi rupture discs and a 6 cm target distance were used. For tobacco leaf and maize immature embryos, 650 psi and 6 cm target distance bombardment conditions were used.
The procedure followed has been described previously (Jefferson, 1987). The X-gluc substrate was from Biosynth. Right after bombardment, bombarded tissues were soaked in the X-gluc solution overnight at 37° C.
Onion epidermis tissues were submerged in 10 mL of MS liquid media to which 100 μL of a 5 mg mL−1 of fluorescein diacetate (Alfa Aesar) solution in acetone were added. The samples were incubated for 2-5 minutes and directly observed under the green channel filter of the fluorescence microscope. For quantitative measurements, 12 images (10.5×8 mm) were taken with the 5× microscope objective scattered every 0.5 cm (4 columns×3 rows). The total amount of dead cells found on each image was counted.
Bright field and fluorescence images were taken using a Zeiss Axio star plus microscope. The objectives used were A-plan 5×/0.12 and 10×/0.25. For the green channel fluorescence images a GFP BP filter (Chroma Technology Corp.) was used (λex=470 nm, beam splitter=495 nm and λem=525 nm). For the red channel, a Texas Red filter (Chroma Technology Corp.) was used (λex=560 nm, beam splitter=595 nm and λem=645 nm). Images were taken with the ProgRes Capture Pro 2.6 software and the ProgRes C3 digital camera (Jenoptik). False magenta color of red channel images was edited using Adobe Photoshop.
Delivery of a Chemical Lyophilized onto Microparticles
The gene gun used to deliver different molecules to plant cells is the PDS1000/He gun from BioRad, a gene gun routinely used in plant transformation experiments. Usually, gold microparticles or microcarriers (0.6-1 μm diameter) are coated with plasmid DNA following a CaCl2/Spermidine precipitation protocol (Frame et al., 2000). These projectiles are suspended in an ethanol solution and poured over the surface of a “macrocarrier set”, composed of a plastic macrocarrier arranged in a macrocarrier holder (
Lyophilization or freeze drying consists in the removal of a solvent from a frozen sample by sublimation and is routinely used to preserve labile molecules like proteins (Tang and Pikal, 2004). Hypothetically, any molecule could be coated onto the surface of the microcarriers after lyophilizing a solution containing the molecule of interest and microparticles, e.g., 0.6 μm gold particles (
The dye bromophenol blue was used to test the ability of the system to deliver a chemical. As a control, a saturated solution of the dye (10 μL of a 100 μg μL−1 mg) was deposited with a pipette on top of onion epidermis tissue. The dye did not permeate and remained outside the cells (
Delivery of DNA Lyophilized onto Microparticles
Delivery of plasmid DNA to plant cells is a routine technique used in plant biotechnology. As mentioned, plasmid DNA is usually coated onto microcarriers following a calcium chloride and spermidine based DNA precipitation protocol. In theory, lyophilizing plasmid DNA onto the surface of microcarriers could also be used as a coating protocol. The plasmid pLMNC95 (Luke Mankin and Thompson, 2001) for GFP expression was mixed with 0.6 μm gold particles and, as in the case of bromophenol blue, a lyophilization process was followed to coat the microparticles over a macrocarrier set. These macrocarriers were used to bombard different plant tissues. One day after bombardment, several GFP fluorescent cells could be observed in onion epidermis cells (
Furthermore, coating 0.6 μm gold with linear double stranded DNA (dsDNA) was also tested. The 1.8 Kb linear DNA cassette for mCherry expression was obtained after digestion of plasmid ER-rk (Nelson et al., 2007). Following the same protocol, linear dsDNA was mixed with 0.6 μm gold microparticles and the suspension lyophilized in a macrocarrier set. Intracellular delivery of mCherry dsDNA was confirmed when red fluorescent cells were observed 1 day after bombardment in onion epidermis tissue (
Proteolistics: Delivery of Proteins Lyophilized onto Microparticles
Protein delivery can be considered a difficult challenge because proteins could be subjected to denaturation during the delivery process. To prove the delivery of an intact protein through the lyophilization coating method eGFP was chosen. This protein will not fluoresce if denatured (Ward and Bokman, 1982). As a control, to test if the protein could be diffused into plant cells, 10 μL of a 100 ng/μL eGFP solution were incubated for 30 minutes on the surface of intact onion epidermis tissue or bombarded with bare 0.6 μm gold microparticles. No intracellular eGFP detection could be observed (data not shown). Lyophilization of eGFP onto 0.6 μm gold microcarriers was done using 2.5 μL of 1 μg μL−1 solution of the protein and 60 μg of microcarriers per shot. Green fluorescent cells could be observed 30 minutes after bombardment (
To corroborate this protein delivery methodology, tetramethylrhodamine isothiocyanate labeled bovine serum albumin (TRITC-BSA) was used. Per shot, 10 μL of a 25 μg μL−1 solution were mixed with 60 μg of 0.6 μm gold microcarriers. This suspension was distributed over the center of a macrocarrier set (
The high concentration of protein used per shot (250 μg per shot) and the lyophilization process could cause cell death by: (1) physical damage of the potential clumps produced after lyophilizing protein and microparticles and (2) excess of intracellular protein delivery reaching toxic levels. To assess cellular death, onion epidermis tissues were stained with fluorescein diacetate, which stains in fluorescent green the living cells, while the dead ones remain dark (
The next step was to assess the delivery of enzymes and confirm activity in cells. β-glucuronidase (275 μg per shot) was lyophilized onto 0.6 μm gold projectiles and onion epidermis tissue was bombarded. Right after bombardment samples were incubated in an X-gluc solution for histochemical staining. One day after bombardment several blue cells could be observed in the bombarded samples (
As another example of active enzyme delivery, trypsin and RNAse A were chosen. These enzymes, if active, should cause cell death when delivered in enough amounts to digest intracellular protein or RNA content. The number of dead cells was measured using fluorescein diacetate staining in samples not bombarded, bombarded only with 0.6 μm gold or with 200 μg of trypsin or 40 μg of RNAse per shot. An optical field obtained with a microscope 5× objective for each treatment is presented in
In theory, any compatible mixed solution of chemicals and biomolecules could be lyophilized onto the projectiles. To assess the co-delivery of two biomolecules, GFP expression plasmid pLMNC95 and TRITC-BSA protein were lyophilized onto 0.6 μm gold. One day after bombardment, the co-delivery of both biomolecules could be observed in the same cells (
Lyophilizing protein, chemicals and other biomolecules along with microparticles offers a projectile coating method for the efficient intracellular delivery of these molecules through the biolistic method. For more than two decades the main purpose of the biolistic method has been the delivery of DNA. This methodology has been broadly used by biologists to manipulate cell genomes for basic and applied research. Recently, new molecule delivery methodologies like cell penetrating peptides or nanotechnology mediated delivery are being developed. The main purpose of these methodologies is the delivery of other biomolecules, in particular, the delivery of proteins (Chugh et al., 2009; Ravichandran, 2009; Lu et al., 2010; Martin-Ortigosa et al., 2012a). These methodologies require the design and synthesis of the vehicles (peptides or nanoparticles) that will allow the delivery of the molecules what could be complicated. Additionally, in the case of nanoparticle mediated protein delivery, nanoparticle surfaces can interact with the proteins affecting the structure or activity upon adsorption (Kane and Stroock, 2007) or remain stuck in the nanoparticle pores (Martin-Ortigosa et al., 2012a).
On the contrary, lyophilization based protein coating of microprojectiles, “Proteolistics,” is a simple and straight forward method that offers an easy, cost effective and quick alternative for the delivery of any protein or molecule combination that can be delivered by the biolistic method to the required target tissue. In this work, we have shown the biolistic delivery to different plant tissues of chemical dyes like bromophenol blue (
In order to expand biolistic methodology uses, dyes have been delivered to different tissues (Gan et al., 2000; Bothwell et al., 2006; Roizenblatt et al., 2006; O'Brien and Lummis, 2007) by the diolistic approach. In this case, a dye is precipitated over the surface of a microparticle after evaporation of the dye solvent. Then, these dye covered microparticles are used as projectiles to deliver the chemical to cells upon bombardment. In this research, lyophilization has been used for the successful delivery of bromophenol blue, and it also has been applied to other labile biomolecules like proteins (
In the experiments described above, saturated solutions of molecules have been used to be mixed with the 0.6 μm gold microparticles. While in some embodiments the delivery of a high concentration of a macromolecule such as a protein may be desired, because this methodology does not offer a controlled release, there may be a concentration that could be toxic for a cell.
Plant genome editing or enzyme assisted plant transformation are hot topics in plant sciences since a precise engineering of the genome is highly desirable. For instance, Wu and colleagues have developed methodologies in which transposon and transposase complexes are attached using different approaches to gold microparticles to improve plant transformation (Wu et al., 2011a; Wu et al., 2011b). As described herein, the simple combination of protein and DNA lyophilized with the microparticles can effectively deliver both molecules to plant tissues upon bombardment, without modifying the enzyme, the DNA sequence or the gold microparticle. This result indicates that proteolistics could be routinely used for these enzyme assisted gene editing purposes.
This methodology could also be applied for the biolistic delivery of molecules to other organisms (Obregón-Barboza et al., 2007), or even be useful for biomedical purposes (Davidson et al., 2000; Yager et al., 2009; Davtyan et al., 2012). This coating methodology may be applied to any type of projectile and to any type of compatible molecule combination.
Previously, protein and 0.6 μm gold projectiles were mixed and poured on top of a macrocarrier of the gene gun, frozen in liquid nitrogen, and lyophilized or freeze-dried. The same experiment was repeated by pouring the protein/0.6 μm gold mixture over a macrocarrier and leaving it to dry on top of a bench for 1 hour or overnight, termed “air-dried” (
The results showed that, in both cases, protein delivery was achieved (
Delivery of Proteins Air-Dried Directly onto Macrocarriers (without Gold Projectiles).
An experiment was conducted using β-glucuronidase and DS-RED2, air-dried directly onto the macrocarriers without 0.6 μm gold projectiles (
Bombardment of Tissues with Liquid Solutions of the Proteins.
As a control, β-glucuronidase and DS-RED2 protein solutions were poured over the macrocarrier and onion epidermis cell tissues were bombarded. Tissue damage was caused by the liquid propelling (
Delivery of DNA Air-Dried onto 0.6 μm Gold Projectiles.
An experiment was conducted to compare DNA coating based on CaCl2/spermidine precipitation, freeze-drying or air-drying, and 0.6 μm gold particles. As seen in
Delivery of DNA Air-Dried or Freeze-Dried Directly onto Macrocarriers (without Gold Projectiles).
A DNA containing solution was freeze-dried or air-dried (without 0.6 μm gold projectiles) directly onto macrocarriers. Onion epidermis tissues were bombarded and 1 to 20 fluorescent cells could be observed (
A mixture of 90 μg of 0.6 μm gold and 200 μg of β-glucuronidase per shot were air-dried onto the macrocarrier set for 2 hours. Ear pinnas of 8 week old CD-1 mice were dissected right after animals were euthanized. The tissues were disposed with the inner part of the ear upwards on an agar medium plate (
To prove the technique in other type of particles, tungsten particles were used. M5 (0.4 μm in size) and M17 (1.1 μm in size) tungsten particles from Bio-Rad were used. 100 μg of each type of particle and 200 μg of β-glucuronidase enzyme or 1 μg of GFP expressing plasmid pLMNC95 were used per shot. The suspensions were air dried for 2 hours onto the macrocarrier-set. Onion epidermis tissues were bombarded at 1100 psi/6 cm. Samples bombarded with β-glucuronidase were incubated overnight at 37° C. in X-gluc solution as previously described. After this incubation period, several cells were showing blue coloration due to active β-glucuronidase enzyme delivery air-dried onto M5 tungsten (
Green fluorescent cells could be observed 1 day after bombardment in the onion epidermis tissues bombarded with the GFP expressing plasmid pLMNC95 air dried onto M5 tungsten (
Sixty μg of gold particles (0.6 μm in diameter) were coated with 1 μg of DNA following the calcium chloride-spermidine protocol (
As seen in
This application claims the benefit of the filing date of U.S. application Ser. No. 61/708,329, filed on Oct. 1, 2012, the disclosure of which is incorporated by reference herein.
| Number | Date | Country | |
|---|---|---|---|
| 61708329 | Oct 2012 | US |
