Claims
- 1. A method of detecting the presence of a polypeptide in a sample, wherein the polypeptide is encoded by a nucleic acid molecule encoding an amyloid precursor mutein, wherein the nucleic acid molecule comprises a nucleic acid sequence encoding a marker and a nucleic acid sequence encoding about 419 amino acid residues of the APP-695 isoform, about 475 amino acid residues of the APP-751 isoform or about 494 amino acid residues of the APP-770 isoform, wherein the nucleic acid molecule is an XbaI-SalI fragment of the gene encoding an amyloid precursor protein isoform, the method of detecting the presence of the polypeptide comprising the steps of:
- (a) contacting the sample with an antibody, which specifically binds the marker under suitable conditions to favor the formation of an antibody-antigen complex, and
- (b) detecting the presence of any complex so formed.
- 2. The method according to claim 1, wherein the amyloid precursor mutein is selected from the group consisting of pCLL602 which is identified as Sequence I.D. No. 6, pCLL603, pCLL605, pCLL606, pCLL608, pCLL609, pCLL610, pCLL611, pCLL612, pCLL613, pCLL621 which is identified as Sequence I.D. No. 8, pCLL918, pCLL919, pCLL962, pCLL964, pCLL987 and pCLL989.
- 3. A method of detecting the presence of a polypeptide in a sample, wherein the polypeptide is encoded by a nucleic acid molecule encoding an amyloid precursor mutein, wherein the nucleic acid amolecule comprises a nucleic acid sequence encoding a marker and a nucleic acid sequence encoding about 419 amino acid residues of the APP-695 isoform, about 475 amino acid residues of the APP-751 isoform or about 494 amino acid residues of the APP-770 isoform and wherein the amino acid residues from position 11 to position 28 are deleted from the portion of the nucleic acid sequence encoding the entire .beta.-amyloid protein domain and wherein the nucleic acid molecule is an XbaI-SalI fragment of the gene encoding an amyloid precursor protein isoform, the method of detecting the presence of the polypeptide comprising the steps of:
- (a) contacting the sample with an antibody which specifically binds the marker under suitable conditions to favor the formation of an antibody-antigen complex, and
- (b) detecting the presence of any complex so formed.
- 4. The method according to claim 3, wherein the amyloid precursor mutein is selected from the group consisting of pCLL604, pCLL607, pCLL920, pCLL988 and pCLL990.
- 5. A method of detecting the presence of a polypeptide in a sample, wherein the polypeptide is encoded by a nucleic acid molecule encoding an amyloid precursor mutein, wherein the nucleic acid molecule comprises a nucleic acid sequence encoding a marker and a nucleic acid sequence encoding about 419 amino acid residues of the APP-695 isoform, about 475 amino acid residues of the APP-751 isoform or about 494 amino acid residues of the APP-770 isoform in which an alanine substitution at a phosphorylation site within the cytoplasmic domain of an amyloid precursor protein is encoded, wherein the nucleic acid molecule is an XbaI-SalI fragment of the gene encoding an amyloid precursor protein isoform, the method of detecting the presence of the polypeptide comprising the steps of:
- (a) contacting the sample with an antibody, which specifically binds the marker under suitable conditions to favor the formation of an antibody-antigen complex, and
- (b) detecting the presence of any complex so formed.
- 6. The method according to claim 5, wherein the amyloid precursor mutein is selected from the group consisting of pCLL614, pCLL615, pCLL616, pCLL626, pCLL627, pCLL628, pCLL629, pCLL630 and pCLL631.
RELATED U.S. APPLICATION DATA
This is a divisional of copending application(s) Ser. No. 08/123,659 filed on Sep. 20, 1993, which is a continuation-in-part of application Ser. No. 07/877,675 filed on May 1, 1992 now abandoned.
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3318793 |
Oct 1993 |
AUX |
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EPX |
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Divisions (1)
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Number |
Date |
Country |
Parent |
123659 |
Sep 1993 |
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Continuation in Parts (1)
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Number |
Date |
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Parent |
877675 |
May 1992 |
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