Claims
- 1. A method of recovering soluble intercellular adhesion molecule-1 (sICAM-1, having the amino acid sequence set forth in FIG. 1) in substantially pure form comprising the steps of:
- (A) removing the supernatant from unlysed cells,
- (B) introducing the supernatant to an affinity matrix containing immobilized antibody which binds to sICAM-1,
- (C) permitting said sICAM-1 to bind to said antibody of said matrix,
- (D) washing said affinity matrix to remove unbound contaminants, and
- (E) recovering said sICAM-1 in substantially pure form by eluting said sICAM-1 from said matrix.
- 2. The method of claim 1, wherein the immobilized antibody of step (B) is selected from the group consisting of antibodies against ICAM-1 and sICAM-1.
- 3. The method of claim 1 further including the step of introducing the supernatant to a wheat germ agglutinin column.
- 4. The method of claim 3 wherein the supernatant is introduced to said wheat germ agglutinin column before the supernatant is introduced to said antibody column.
- 5. The method of claim 3 wherein the supernatant is introduced to said wheat germ agglutinin column after the supernatant is introduced to said antibody column.
- 6. The method of claim 1 or 2 additionally comprising a purification step selected from the group consisting of velocity sedimentation through sucrose gradients, sizing chromatography, gel electrophoresis, and ion chromatography.
Parent Case Info
This is a divisional of copending application U.S. Ser. No. 08/425,989 filed Apr. 20, 1995, which is a continuation of U.S. Ser. No. 08/156,653 filed Nov. 22, 1993, now abandoned, which is a continuation of U.S. Ser. No. 08/005,204 filed Jan. 15, 1993, now abandoned, which is a continuation of U.S. Ser. No. 07/449,356 filed Dec. 21, 1989, now abandoned, which is a continuation-in-part of U.S. Ser. No., and a continuation-in-part of U.S. Ser. No. 07/445,951 filed Dec. 13, 1989, now abandoned, which is a continuation-in-part of U.S. Ser. No. 07/301,192 filed Jan. 24, 1989, which issued as U.S. Pat. No. 5,235,049 on Aug. 10, 1993.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
5235049 |
McClelland et al. |
Aug 1993 |
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Divisions (1)
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Number |
Date |
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Parent |
425989 |
Apr 1995 |
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Continuations (3)
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156653 |
Nov 1993 |
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Parent |
05204 |
Jan 1993 |
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Parent |
449356 |
Dec 1989 |
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Continuation in Parts (2)
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Parent |
445951 |
Dec 1989 |
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Parent |
301192 |
Jan 1989 |
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