Claims
- 1. A method of producing high density arrays of target substances comprising sectioning a bundle of target-strands, where the target-strands comprise the target substances, and where the sectioning results in a high density array of target substances present in three Cartesian axes.
- 2. The method of claim 1, where the sectioning is performed with a cutting device selected from the group consisting of a microtome, laser, saw, and hot wire.
- 3. The method of claim 1, where the sectioning is performed such that the resultant high density array has a thickness of from about 0.1 μm to about 1.0 mm.
- 4. The method of claim 1, where the sectioning is performed such that the resultant high density array has a thickness of greater than 50 μm.
- 5. The method of claim 1, further including stabilizing the bundle.
- 6. The method of claim 5, where stabilizing is performed by embedding the bundle in a material selected from the group consisting of epoxy, polypropylene and polystyrene.
- 7. The method of claim 1, where the bundle in the sectioning step comprises target-strands selected from the group consisting of a cast rod of target substance, a target substance absorbed onto a glass fiber, a target substance absorbed onto a silk thread, a target substance attached to a polymer fiber, a target substance embedded in a porous rod, a target substance coated on a metal wire, a target substance contained within a matrix of gelatin, a line of a target substance drawn on a glass slide, a line of a target substance drawn on a membrane, and a target substance attached to the inside of a tube.
- 8. The method of claim 1, where at least one of the target substances comprising the sectioned bundle of target-strands is selected from the group consisting of DNA, RNA, peptides, proteins, glycoproteins, lipoproteins, carbohydrates, lipids and immunoglobulins.
- 9. The method of claim 1, where at least one of the target substances comprising the sectioned bundle of target-strands is selected from the group consisting of zinc, sulfur and gold.
- 10. The method of claim 1, where at least one of the target substances comprising the sectioned bundle of target-strands is selected from the group consisting of viruses, chromosomes, mitochondria, prokaryotic cells, archaebacteria and eukaryotic cells.
- 11. The method of claim 1, where at least one of the target substances comprising the sectioned bundle of target-strands is selected from the group consisting of ceramics, glasses, plastics, polymeric materials, wood, fabric and concrete.
- 12. The method of claim 1, where at least one of the target substances comprising the sectioned bundle of target-strands is selected from the group consisting of semiconductors and superconductors
- 13. The method of claim 1, further including incorporating a material in addition to the target-strands into the bundle.
- 14. The method of claim 13, where the additional material is an antioxidant.
- 15. The method of claim 13, where the additional material is a microbial inhibitor.
- 16. The method of claim 13, where the additional material is a nonfluorescent counterstain.
- 17. The method of claim 13, where the additional material is a reflecting substance.
- 18. The method of claim 13, where the additional material is a secondary enzyme.
- 19. A method of interrogating a high density array, comprising producing a high density array according to the method of claim 1 and then interrogating the array.
- 20. The method of claim 19, where interrogating comprises visual inspection.
- 21. The method of claim 19, where interrogating comprises chemical deposition.
- 22. The method of claim 19, where interrogating comprises electrical probing.
- 23. The method of claim 19, where interrogating comprises magnetic sensing.
- 24. The method of claim 19, where interrogating comprises mechanical sensing.
CROSS-REFERENCE TO RELATED APPLICATION
[0001] This Application is a divisional of U.S. patent application Ser. No. 09/827,505 filed Apr. 6, 2001 and titled “Method of Making High Density Arrays,” which is a continuation of U.S. patent application Ser. No. 09/145,140 filed Aug. 28, 1998 and titled “Method of Making High Density Arrays,” which is a divisional of U.S. patent application Ser. No. 08/927,974 titled “Method of Making High Density Arrays,” filed Sep. 11, 1997, now abandoned, the contents of which are incorporated herein by reference in its entirety.
Divisions (2)
|
Number |
Date |
Country |
Parent |
09827505 |
Apr 2001 |
US |
Child |
10317927 |
Dec 2002 |
US |
Parent |
08927974 |
Sep 1997 |
US |
Child |
09145140 |
Aug 1998 |
US |
Continuations (1)
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Number |
Date |
Country |
Parent |
09145140 |
Aug 1998 |
US |
Child |
09827505 |
Apr 2001 |
US |