Claims
- 1. A method of measuring X-prolyl dipeptidyl-aminopeptidase activity which comprises contacting a compound having the structure:
- X-L-proline-Y
- or an acid salt thereof with said X-prolyl dipeptidyl-aminopeptidase in an aqueous medium at pH 7 to 9 under conditions suitable for the enzymatic reaction to occur and assaying the enzymatically liberated Y-H, said X being a residue of an amino acid selected from the group consisting of glycine, alanine, valine, leucine, serine, threonine, phenylalanine, tyrosine, tryptophane, glutamic acid, aspartic acid, lysine and arginine; and said Y being a residue of said Y-H selected from the group consisting of p-nitroaniline, p-phenylazoaniline, and 4-phenylazo-1-naphthylanine.
- 2. A method as set forth in claim 1, wherein human serum is used as the source of said X-prolyl dipeptidyl-aminopeptidase.
- 3. A method as set forth in claim 2 wherein the said compound is glycyl-L-proline p-nitroanilide.
- 4. A method as set forth in claim 2 wherein the said compound is glycyl-L-proline p-nitroanilide tosylate.
- 5. A method as set forth in claim 2 wherein the said compound is L-lysyl-L-proline p-nitroanilide.
- 6. A method as set forth in claim 2 wherein the said compound is L-arginyl-L-proline p-nitroanilide.
- 7. A method as set forth in claim 2 wherein the said compound is L-alanyl-L-proline p-nitroanilide.
- 8. A method as set forth in claim 2 wherein the said compound is L-glutamyl-L-proline p-nitroanilide.
- 9. A method as set forth in claim 2 wherein the said compound is L-aspartyl-L-proline p-nitroanilide.
- 10. A method for detecting a human disease which disease is characterized by elevated levels of X-prolyl dipeptidyl-aminopeptidase in human serum which comprises measuring the activity of said X-prolyl dipetidyl-aminopeptidase in human serum by contacting a compound having the structure:
- X-L-proline-Y
- or an acid salt thereof with said human serum in an aqueous medium at from 30.degree. to 45.degree. C. at a pH of from 7.5 to 8.9; and assaying enzymatically liberated Y-H; said X being an amino acid selected from the group consisting of glycine, alanine, valine, leucine, serine, threonine, phenylalanine, tyrosine, tryptophane, glutamic acid, aspartic acid, lysine and arginine; and said Y being a residue of said Y-H selected from the group consisting of p-nitroaniline, p-phenylazoaniline, and 4-phenylazo-1-naphthylanine.
- 11. A method as set forth in claim 10 wherein the said compound is glycyl-L-proline p-nitroanilide.
- 12. A method as set forth in claim 10 wherein the said compound is glycyl-L-proline p-nitroanilide tosylate.
- 13. A method as set forth in claim 10 wherein the said compound is L-lysyl-L-proline p-nitroanilide.
- 14. A method as set forth in claim 10 wherein the said compound is L-arginyl-L-proline p-nitroanilide.
- 15. A method as set forth in claim 10 wherein the said compound is L-alanyl-L-proline p-nitroanilide.
- 16. A method as set forth in claim 10 wherein the said compound is L-glutamyl-L-proline p-nitroanilide.
- 17. A method as set forth in claim 10 wherein the said compound is L-aspartyl-L-proline p-nitroanilide.
- 18. A method as set forth in claim 10, wherein said disease is malignant tumor.
- 19. A ,ethod as set forth in claim 10, wherein said disease is a disease selected from the group consisting of gastric cancer, pancreatic cancer, pulmonary cancer, leukemia, lymphosarcoma and Hodgkin's disease.
- 20. A method as set forth in claim 10, said disease is hepatobiliary disease or essential hypertension.
Priority Claims (1)
Number |
Date |
Country |
Kind |
50-130809 |
Oct 1975 |
JPX |
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Parent Case Info
This is a division of application Ser. No. 733,343 filed Oct. 18, 1976, now U.S. Pat. No. 4,119,620.
US Referenced Citations (3)
Number |
Name |
Date |
Kind |
4016042 |
Svendsen |
Apr 1977 |
|
4061625 |
Ekenstum et al. |
Dec 1977 |
|
4070245 |
Svendsen |
Jan 1978 |
|
Non-Patent Literature Citations (2)
Entry |
Hino, et al., "Glycyclprolyl .beta.-Naphthylamidase Activity in Human Serum", Clin. Chim. Acta., vol. 62 (1975), pp. 5-11. |
Pettit, Synthetic Peptides I, (1971), pp. 124-125. |
Divisions (1)
|
Number |
Date |
Country |
Parent |
733343 |
Oct 1976 |
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