Claims
- 1. A method for preparing nucleic acid microchips comprising:
attaching nucleic acid molecules to a first surface of a first chip, and contacting said first surface of said first chip with a first surface of a second chip.
- 2. The method of claim 1, wherein the nucleic acid molecules are DNA.
- 3. The method of claim 1, wherein the nucleic acid molecules are RNA.
- 4. The method of claim 1, wherein the first surface of the second chip is in a relatively liquid state.
- 5. The method of claim 1, wherein the first surface of the second chip comprises a rubber material.
- 6. The method of claim 1, wherein the first surface of the second chip comprises an acrylamide layer.
- 7. The method of claim 1, wherein the first surface of the first chip comprises a nucleic acid surface density of at least 50 pmoles/cm2, more preferably ranging from 50-2000 pmoles/cm2, and most preferably greater than 2000 pmoles/cm2.
- 8. The method of claim 1, wherein the nucleic acid molecules are attached to the first surface of the first chip by disulphide bonds.
- 9. The method of claim 1, wherein the printing temperature is 25° C.
- 10. The method of claim 1, wherein the printing temperature ranges from 25° C.-100° C.
- 11. The method of claim 1, wherein the printing temperature is 95° C., more preferably 99° C., and most preferably 100° C.
- 12. The method of claim 1, wherein the printing temperature is at least 30° C.
- 13. The method of claim 1, wherein the printing time varies from about 10 seconds to about 10 minutes.
- 14. The method of claim 1, wherein the printing time is at least 15 seconds.
- 15. The method of claim 1, wherein the number of print chips generated from a single master chip ranges from 2-200 print chips.
- 16. The method of claim 1, wherein the number of print chips generated from a single master chip is at least two.
- 17. The method of claim 1, wherein the nucleic acid is RNA or DNA.
- 18. A nucleic acid microchip prepared by a method comprising:
attaching nucleic acid molecules to a first surface of a first chip, and contacting said first surface of said first chip with a first surface of a second chip.
- 19. The microchip of claim 18, wherein the nucleic acid molecules are DNA.
- 20. The microchip of claim 18, wherein the nucleic acid molecules are RNA.
- 21. The microchip of claim 18, wherein the first surface of the second chip is in a relatively liquid state.
- 22. The microchip of claim 18, wherein the first surface of the second chip comprises a rubber material.
- 23. The microchip of claim 18, wherein the first surface of the second chip comprises an acrylamide layer.
- 24. The microchip of claim 18, wherein the first surface of the first chip comprises a nucleic acid surface density of at least 50 pmoles/cm2, more preferably ranging from 50-2000 pmoles/cm2, and most preferably greater than 2000 pmoles/cm2.
- 25. The microchip of claim 18, wherein the nucleic acid molecules are attached to the first surface of the first chip by disulphide bonds.
- 26. The microchip of claim 18, wherein the printing temperature is 25° C.
- 27. The microchip of claim 18, wherein the printing temperature ranges from 25° C.-100° C.
- 28. The microchip of claim 18, wherein the printing temperature is 95° C., more preferably 99° C., and most preferably 100° C.
- 29. The microchip of claim 18, wherein the printing temperature is at least 30° C.
- 30. The microchip of claim 18, wherein the printing time varies from about 10 seconds to about 10 minutes.
- 31. The microchip of claim 18, wherein the printing time is at least 15 seconds.
- 32. The microchip of claim 18, wherein the number of print chips generated from a single master chip ranges from 2-200 print chips.
- 33. The microchip of claim 18, wherein the number of print chips generated from a single master chip is at least two
- 34. The microchip of claim 18, wherein the nucleic acid is RNA or DNA.
PRIORITY CLAIM
[0001] This Application for Patent claims the benefit of priority from, and hereby incorporates by reference the entire disclosure of, U.S. Provisional Application for Patent Ser. No. 60/219,376 filed Jul. 19, 2000.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60219376 |
Jul 2000 |
US |