Claims
- 1. A method of estimating fat concentration in a waste fluid sample, the method comprising:
- inducing cytolysis in the waste fluid sample producing a cytolytic fluid;
- variably scattering at a least a portion of light from a light source;
- intercepting the variably scattered light at two comparison chambers where one comparison chamber receives and transmits the cytolytic fluid and a second comparison chamber receives and transmits a reference standard fluid;
- detecting output light emanating from the two comparision chambers;
- comparing light absorption characteristics of the cytolytic fluid with light absorption characteristics of the reference standard fluid; and
- looking up in a table a fat concentration of a reference standard fluid having light absorption characteristics substantially similar to the cytolytic fluid.
- 2. A method of estimating hemoglobin concentration in a waste fluid sample, the method comprising:
- processing erythrocytes in the waste fluid sample including subjecting the erythrocytes to cytolysis producing a cytolytic fluid;
- converting free hemoglobin in the cytolytic fluid to another colored compound;
- after converting the free hemoglobin, comparing light absorption characteristics of the cytolytic fluid with light absorption characteristics of a reference standard; and
- looking up in a table a hemoglobin concentration of a reference standard having light absorption characteristics substantially similar to the cytolytic fluid.
- 3. The method of claim 2 where the step of converting includes chemically converting the free hemoglobin.
- 4. The method of claim 2 where the step of converting includes mixing the waste fluid sample with Drabkin's solution.
- 5. The method of claim 2 where the step of processing includes diluting the waste fluid sample with distilled water.
- 6. The method of claim 2 where the step of processing includes subjecting erythrocytes in the waste fluid sample to osmotic cytolysis.
- 7. The method of claim 2 where the step of processing includes subjecting erythrocytes in the waste fluid sample to complement-induced cytolysis.
- 8. A method of estimating hemoglobin concentration in a waste fluid sample, the method comprising:
- inducing cytolysis of red blood cells in the waste fluid sample producing a cytolytic fluid;
- converting free hemoglobin in the cytolytic fluid to another colored compound; and
- after converting the free hemoglobin, comparing light absorption characteristics of the cytolytic fluid with light absorption characteristics of a reference standard at substantially a same wavelength.
- 9. The method of claim 8 where the step of inducing includes subjecting erythrocytes in the waste fluid sample to osmotic cytolysis.
- 10. The method of claim 8 where the step of inducing includes subjecting erythrocytes in the waste fluid sample to complement-induced cytolysis.
CONTINUATION INFORMATION
This application is a division of application Ser. No. 08/645,349, filed May 13, 1996, now U.S. Pat. No. 5,709,670, which is a continuation-in-part of PCT Application Serial No. PCT/US95/14101 filed Oct. 31, 1995, which is a continuation-in-part of application Ser. No. 08/484,877 filed Jun. 7, 1995, now U.S. Pat. No. 5,522,805, which is a continuation-in-part of application Ser. No. 08/237,350 filed May 3, 1994, now U.S. Pat. No. 5,492,537.
US Referenced Citations (65)
Foreign Referenced Citations (1)
Number |
Date |
Country |
WO 9218049 |
Oct 1992 |
WOX |
Non-Patent Literature Citations (3)
Entry |
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Divisions (1)
|
Number |
Date |
Country |
Parent |
645,349 |
May 1996 |
|
Continuation in Parts (3)
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Number |
Date |
Country |
Parent |
PCT/US95/14101 |
Oct 1995 |
|
Parent |
484,877 |
Jun 1995 |
|
Parent |
237,350 |
May 1994 |
|