Patent Application
20230060581
This invention relates to treatment of pancreatic cancer.
All publications herein are incorporated by reference to the same extent as if each individual publication or patent application was specifically and individually indicated to be incorporated by reference. The following description includes information that may be useful in understanding the present invention. It is not an admission that any of the information provided herein is prior art or relevant to the presently claimed invention, or that any publication specifically or implicitly referenced is prior art.
Pancreatic cancer (PC) has a 5-year survival of 9% and is projected to be the second leading cause of cancer-related mortality in the United States before 2030. FDA approved therapies specifically for PC are limited to combinatorial cytotoxic regimens including FOLFIRINOX, gemcitabine with nab-paclitaxel, and nanoliposomal irinotecan with 5-fluorouracil. Despite the advent of precision medicine, olaparib as maintenance therapy in germline BRCA-mutated patients is the sole FDA approved targeted therapeutic. In addition, two classes of tumor agnostic targeted therapies include pancreatic cancer. PD-1 inhibitors have been approved for MSI-high or MMR deficient solid tumors, however, these types of tumors represent approximately 0.5-1% of pancreatic cancers. NTRK inhibitors are also FDA approved for TRK gene fusions, which are very rare in pancreatic cancer. As such, there remains an urgent need in the art for treatments for pancreatic cancer.
The following embodiments and aspects thereof are described and illustrated in conjunction with compositions and methods which are meant to be exemplary and illustrative, not limiting in scope.
Various embodiments of the present invention provide for a method of treating pancreatic cancer in a subject in need thereof, comprising administering one or more MAPK pathway inhibitors to the subject, wherein the subject has a mutation in one or more genes in the MAPK signaling pathway.
In various embodiments, the subject can be KRAS wild type.
In various embodiments, the mutation can be in BRAF. In various embodiments, the mutation can be selected from: (a) BRAF V600E, (b) oncogenic fusion of BRAF and another gene, (c) non-V600 mutation, insertion or deletion, or (d) a mutation other than (a)-(c) with an additional driver mutation. In various embodiments, the mutation is BRAF N486 P490del.
In various embodiments, the subject can have a mutation in BRAF, and the one or more MAPK pathway inhibitors is a BRAF inhibitor, a MEK inhibitor or both.
In various embodiments, the one or more MAPK pathway inhibitors can be a MEK inhibitor. In various embodiments, the MEK inhibitor can be trametinib, cobimetinib, binimetinib, selumetinib, or mirdametinib. In various embodiments, the MEK inhibitor is Cobimetinib, Binimetinib, or Trametinib. In various embodiments, one or more MAPK pathway inhibitors can be dabrafenib mesylate and trametinib dimethyl sulfoxide, cobimetinib fumarate, binimetinib, binimetinib and encorafenib, trametinib dimethyl sulfoxide, selumetinib sulfate, LNP-3794, dabrafenib mesylate, panitumumab and trametinib dimethyl sulfoxide, HL-085, mirdametinib, MK-2206 and selumetinib sulfate, trametinib dimethyl sulfoxide and uprosertib, ATI-450, ATR-002, CKI-27, CS-3006, durvalumab and selumetinib sulfate, E-6201, FCN-159, SHR-7390, TQB-3234, ABM-1383, ATR-004, ATR-005, ATR-006, EBI-1051, KZ-001, OTS-514, OTS-964, Small Molecule to Inhibit MKK4 for Acute Liver Failure and Chronic Liver Disease, Small Molecules to Inhibit Mitogen Activated Protein Kinase Kinase for Oncology, or SNR-1611.
In various embodiments, the one or more MAPK pathway inhibitors can be a BRAF inhibitor. In various embodiments, the BRAF inhibitor is dabrafenib mesylate and trametinib dimethyl sulfoxide, sorafenib tosylate, binimetinib and encorafenib, dabrafenib mesylate, encorafenib, vemurafenib, sorafenib tosylate, dabrafenib mesylate, panitumumab and trametinib dimethyl sulfoxide, hydroxychloroquine and sorafenib tosylate, lifirafenib maleate, TAK-580, BAL-3833, belvarafenib, CKI-27, LUT-014, LXH-254, RXDX-105, TQB-3233, XP-102, UB-941, ABM-1310, AFX-1251, APL-102, ARI-4175, AZ-304, BGB-3245, INU-152, LYN-204, PV-103, REDX-05358, or SJP-1601.
In various embodiments, the BRAF inhibitor can be Dabrafenib, Vemurafenib, Encorafenib, Lifirafenib, belvarafenib, or sorafenib tosylate. In various embodiments, the BRAF inhibitor can be Vemurafenib, Dabrafenib or Encorafenib, or combinations thereof.
In various embodiments, the one or more MAPK pathway inhibitors can be Dabrafenib and trametinib, Vemurafenib and cobimetinib, Trametinib, or Vemurafenib. In various embodiments, the one or more MAPK pathway inhibitor can be Binimetinib and Encorafenib. In various embodiments, the one or more MAPK pathway inhibitors can be Vemurafenib and the method further comprises administering carboplatin, paclitaxel or both. In various embodiments, the one or more MAPK pathway inhibitors can be Trametinib and the method further comprises administering Pembrolizumab.
In various embodiments, the method can further comprise administering one or more chemotherapy drugs, one or more PD-1 inhibitors or PD-L1 inhibitors, both a chemotherapy drug and a PD-1 inhibitor, or both a chemotherapy drug and a PD-L1 inhibitor.
In various embodiments, the one or more MAPK pathway inhibitor can be Binimetinib and Encorafenib, wherein the mutation is BRAF V600E, and the pancreatic cancer is BRAF V600E mutated pancreatic ductal adenocarcinoma (PDAC).
In various embodiments, the subject's pancreatic cancer has progressed on at least one line of therapy for metastatic disease or wherein the subject can be intolerant of at least one line of therapy for metastatic disease.
In various embodiments, the subject's pancreatic cancer has recurred with metastatic disease less than or equal to 12 weeks of completion of neoadjuvant or adjuvant systemic chemotherapy, or wherein the subject has locally advanced pancreatic cancer whose pancreatic cancer progressed to metastatic disease less than or equal to 12 weeks after completion of systemic chemotherapy, or wherein the subject's pancreatic cancer has recurred with metastatic disease less than or equal to 12 weeks of completion of systemic chemotherapy.
Other features and advantages of the invention will become apparent from the following detailed description, taken in conjunction with the accompanying drawings, which illustrate, by way of example, various features of embodiments of the invention.
Exemplary embodiments are illustrated in referenced figures. It is intended that the embodiments and figures disclosed herein are to be considered illustrative rather than restrictive.
All references cited herein are incorporated by reference in their entirety as though fully set forth. Unless defined otherwise, technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Singleton et al., Dictionary of Microbiology and Molecular Biology 3rd ed., Revised, J. Wiley & Sons (New York, N.Y. 2006); March, Advanced Organic Chemistry Reactions, Mechanisms and Structure 7th ed., J. Wiley & Sons (New York, N.Y. 2013); and Sambrook and Russel, Molecular Cloning: A Laboratory Manual 4th ed., Cold Spring Harbor Laboratory Press (Cold Spring Harbor, N.Y. 2012), provide one skilled in the art with a general guide to many of the terms used in the present application.
One skilled in the art will recognize many methods and materials similar or equivalent to those described herein, which could be used in the practice of the present invention. Indeed, the present invention is in no way limited to the methods and materials described. For purposes of the present invention, the following terms are defined below.
As used herein the term “about” when used in connection with a referenced numeric indication means the referenced numeric indication plus or minus up to 5% of that referenced numeric indication, unless otherwise specifically provided for herein. For example, the language “about 50%” covers the range of 45% to 55%. In various embodiments, the term “about” when used in connection with a referenced numeric indication can mean the referenced numeric indication plus or minus up to 4%, 3%, 2%, 1%, 0.5%, or 0.25% of that referenced numeric indication, if specifically provided for in the claims.
As used herein, a “subject” means a human or animal. Usually the animal is a vertebrate such as a primate, rodent, domestic animal or game animal. Primates include chimpanzees, cynomologous monkeys, spider monkeys, and macaques, e.g., Rhesus. Rodents include mice, rats, woodchucks, ferrets, rabbits and hamsters. Domestic and game animals include cows, horses, pigs, deer, bison, buffalo, feline species, e.g., domestic cat, and canine species, e.g., dog, fox, wolf. The terms, “patient”, “individual” and “subject” are used interchangeably herein. In an embodiment, the subject is mammal. The mammal may be a human, non-human primate, mouse, rat, dog, cat, horse, or cow, but are not limited to these examples. In addition, the methods described herein may be used to treat domesticated animals and/or pets. In some embodiments, the subject is a human. In some embodiments, the subject is a male subject. In some embodiments, the subject has a cancer. In some embodiments, the subject has a tumor.
A subject may be one who has been previously diagnosed with or identified as suffering from or having a disease, disorder or condition in need of treatment or one or more complications related to the disease, disorder, or condition, and optionally, have already undergone treatment for the disease, disorder, or condition or the one or more complications related to the disease, disorder, or condition. Alternatively, a subject can also be one who has not been previously diagnosed as having a disease, disorder, or condition or one or more complications related to the disease, disorder, or condition. For example, a subject may be one who exhibits one or more risk factors for a disease, disorder, or condition or one or more complications related to the disease, disorder, or condition or a subject who does not exhibit risk factors. A “subject in need” of treatment for a particular disease, disorder, or condition may be a subject suspected of having that disease, disorder, or condition, diagnosed as having that disease, disorder, or condition, already treated or being treated for that disease, disorder, or condition, not treated for that disease, disorder, or condition, or at risk of developing that disease, disorder, or condition.
“Mammal” as used herein refers to any member of the class Mammalia, including, without limitation, humans and nonhuman primates such as chimpanzees, and other apes and monkey species; farm animals such as cattle, sheep, pigs, goats and horses; domestic mammals such as dogs and cats; laboratory animals including rodents such as mice, rats and guinea pigs, and the like. The term does not denote a particular age or sex. Thus adult and newborn subjects, as well as fetuses, whether male or female, are intended to be including within the scope of this term.
“Therapeutically effective amount” as used herein refers to that amount which is capable of achieving beneficial results in a patient with pancreatic cancer. A therapeutically effective amount can be determined on an individual basis and will be based, at least in part, on consideration of the physiological characteristics of the mammal, the type of delivery system or therapeutic technique used and the time of administration relative to the progression of the disease.
“Treatment” and “treating,” as used herein refer to both therapeutic treatment and prophylactic or preventative measures, wherein the object is to prevent, slow down and/or lessen the disease even if the treatment is ultimately unsuccessful.
“Disease progression” as used herein refers to clinical or radiographic disease progression, as defined by RECIST 1.1 criteria.
“Progression-free survival” (PFS) as used herein refers to the time from the first administration of the inventive therapy described herein to the first of either disease progression or death from any cause, where disease progression is determined based on RECIST 1.1 criteria. PFS will be estimated using the Kaplan-Meier method. The median PFS and corresponding 95% confidence interval will be reported. Patients who do not experience disease progression or death while on protocol will be censored at the last disease assessment date.
“Overall survival” (OS) as used herein refers to the time from the first administration of the inventive therapy described herein to death from any cause.
“Duration of response” as used herein refers to the duration of time from first documentation of an objective response to the earliest date disease progression is documented or death from any cause.
“Time to response” as used herein refers to the duration of time from the first administration of the inventive therapy described herein to the first documentation of an objective response.
PC sequencing efforts by the International Cancer Genome Consortium (ICGC), The Cancer Genome Atlas (TCGA) and others have identified a preponderance of KRAS mutations (92-93%). In those lacking KRAS alterations, driver mutations have been identified including: BRAF, GNAS, CTNNB1, ROS, NRG1, and ALK fusions. BRAF mutations have been previously identified in KRAS wild-type (WT) and mutant pancreatic cancer cell lines and patient tumors.
Alterations in BRAF are classified into 3 functional groups based on the dependence of RAS activation, BRAF kinase activity, and signaling properties. Class I BRAF mutations result in RAS-independent kinase activation and signal as monomers, e.g., BRAF V600E. Class II mutations are also RAS-independent but signal as heterodimers and rarely co-occur with other alterations in the MAPK pathway. This group includes activating BRAF mutations, fusions, and in-frame deletions. Class III mutations are RAS dependent and occur when they heterodimerize with wild-type CRAF and amplify RAS signaling, often co-occurring with activating RAS or NF1 loss-of-function mutations.
Beyond PC, recurrent BRAF alterations are observed across multiple cancer types including lung, colon, thyroid, and melanoma, as well as in non-Hodgkin lymphoma. In pancreatic acinar cell carcinomas, rearrangements involving BRAF and RAF1 (CRAF) have been identified in approximately 23% of tumors. Combined BRAF and MEK inhibition is now FDA approved for metastatic melanoma, non-small cell lung cancer and anaplastic thyroid cancer. In addition, targeting BRAF V600 alterations with combinations of RAF/MEK inhibition and anti-EGFR therapy has improved outcomes in colorectal cancer and received breakthrough status in 2018.
Commercial and in-house Next Generation Sequencing (NGS) platforms are providing precision medicine in PC. These efforts have identified several potential targets for development. However, there remains a lack of functional studies and outcome data in BRAF mutated PC.
As described herein we address this knowledge gap by examining all reported BRAF alterations in epithelial pancreatic malignancies. To provide clinical support for genomic and preclinical BRAF predictions and therapeutic strategies we have compiled the largest case series of BRAF altered PC, and report outcomes and responses to BRAF directed therapy.
Activation of the RAS/RAF/MEK/ERK pathway is critical for the proliferation, survival, and tumorigenesis in approximately 30% of all malignancies and 90-95% of all pancreatic malignancies. To date, only oncogenic mutations in KRAS have been well characterized for PC. We demonstrate herein that BRAF alterations have biological and clinical relevance in the KRAS WT population. Utilizing large published datasets, and a multi-institutional retrospective case series, we report the first comprehensive evaluation of BRAF alterations in pancreatic cancer, associated clinical outcomes, and evaluate response to BRAF-directed therapy.
Previous studies have identified driver BRAF mutations in KRAS wild-type pancreatic cancer. In our review of all publicly available sequencing PC data we have confirmed BRAF alterations occurred in 2% of pancreatic ductal adenocarcinoma and 25% of pancreatic acinar cell tumors. These alterations in PDAC occurred evenly between Exon 15 (V600), fusions, Exon 11(non-V600), and non-canonical alterations. Exon 15 (V600) alterations were also identified for the first time in a pancreaticoblastoma and a pseudopapillary neoplasm of the pancreas.
We then compiled the largest retrospective series of PC patients with BRAF alterations to date. We confirm that pancreatic cancer is characterized by a unique spectrum of non-exon 15 (V600) variants in the BRAF gene including a recurring five-amino-acid (ΔNVTAP) deletion in the BRAF β3-αC loop (i.e. BRAFAΔNvTAP), and BRAF gene fusions.
In our 81-case cohort, exon 15 and exon 11 mutations, and fusion events were found to be almost entirely exclusive of KRAS alterations (62/63). In individuals with missense mutations, categorized as non-canonical, 11/19 had KRAS mutations. Our findings indicate that BRAF alterations are an important driver in many KRAS WT pancreatic tumors.
In this cohort we confirm reports of clinical responses to MEK and BRAF inhibition in subjects with biologically significant BRAF alterations. Benefit was well aligned with the classification system described by Yaeger et al., as many subjects with class 1 (V600E) and 2 (fusion) abnormalities had enhanced responses. Whereas, we report no responses in individuals with other abnormalities, some of which are considered class 3 variants or were poorly-characterized alterations that were reported as pathogenic by the genomic testing laboratory.
Many of the BRAF alterations found in this cohort have not been fully characterized and were not able to be placed in this functional classification system. For example, BRAF T599 V600insT is a change in the amino acid sequence of the serine/threonine protein kinase B-raf protein where a threonine residue has been inserted between the threonine at position 599 and the valine at position 600. In-vitro experiments have demonstrated high kinase activity and dimerization dependence. However, there are clinical reports of responsiveness to BRAF inhibition in a patient with metastatic melanoma. The BRAF ΔNVTAP deletion was unusually prevalent in our cohort of BRAF alterations 16/81. This short, in-frame 5 amino acid deletion within the kinase domain (β3-αC Loop) which regulates BRAF kinase activity. These alterations result in increased kinase activity but are resistant to the BRAF inhibitor vemurafenib. However, there are clinical case reports of significant activity with dabrafenib in patients with ΔNVTAP deletion which aligns with the observation that dabrafenib fits better than vemurafenib inside the BRAF pocket. In addition, many of the fusions and missense mutations presented here have not been fully characterized. These include: D566E; E26D; E501K; G596R; K601N; N236K; P403Lfs*8; Q94K; R389H; S467L; T241P; T310I; and truncation intron 8.
In our cohort, only individuals receiving approved MEK inhibitors or approved combinations of MEK and BRAF inhibitors had clinical benefit. In a recently published study, 3 patients with pancreatic cancer were enrolled in a 172 patient pan-cancer BRAF V600 study and results were consistent with our findings. Individuals who had received BRAF-directed therapy demonstrated a trend toward improved outcomes vs. those who did not. Notably these BRAF alterations occur across a spectrum of epithelial pancreatic tumors underscoring the importance of routine molecular profiling irrespective of histology across pancreatic cancers, particularly acinar cell carcinomas (which commonly harbor BRAF fusions) and other pancreaticobiliary tumors (e.g. cholangiocarcinoma, ampullary, and duodenal carcinomas).
We examined BRAF categorization as a prognostic or predictive factor. In our cohort, BRAF categorization was not associated with differences in overall survival. Unlike previous reports in colon cancer, BRAF V600E alterations were not predictive of poor response to chemotherapy. However, we found that BRAF fusion abnormalities may speculatively represent a predictive marker of improved response to FOLFIRINOX and poor response to gemcitabine and nab-paclitaxel. We were unable to find any evaluation of chemotherapeutic response to tumors harboring fusion abnormalities outside of pemetrexed therapy in lung cancers with ROS1 fusion abnormalities.
Due to the high unmet need in the PC patient population and the infrequency of BRAF alterations, a single arm prospective trial confirming substantial response rates and durability of responses would likely be sufficient to convince the FDA to extend the indication labelling of the previously FDA approved BRAF and MEK inhibitors to include patients with BRAF-mutated PC.
Herein we have described a cohort of BRAF-mutated pancreatic cancer that comprises 2% of PC cases and can be classified into 4 functional categories. We report promising treatment responses and encouraging outcomes in patients receiving BRAF-directed therapy. These responses were functional class dependent and occurred in Class 1 and 2 abnormalities.
Embodiments of the present invention, are based at least in part, on these findings.
Various embodiments of the present invention provide for a method of treating pancreatic cancer in a subject in need thereof, comprising administering one or more MAPK pathway inhibitors to the subject, wherein the subject has a mutation in one or more genes in the MAPK signaling pathway.
In various embodiments, the subject has been tested and determined to have the mutation in one or more genes in the MAPK signaling pathway. In various embodiments, the method further comprises testing the subject for a mutation in one or more genes in the MAPK signaling pathway prior to administering the one or more MAPK pathway inhibitors to the subject. In various embodiments, the subject's cancer has a mutation in one or more genes in the MAPK signaling pathway. In various embodiments, the pancreatic cancer comprises the mutation in one or more genes in the MAPK signaling pathway.
In various embodiments, administering one or more MAPK pathway inhibitors to the subject imparts a duration of response that is longer as compared to standard of care therapy available at the time of the present invention. In various embodiments, administering one or more MAPK pathway inhibitors to the subject imparts a progression free survival that is longer as compared to standard of care therapy available at the time of the present invention. In various embodiments, administering one or more MAPK pathway inhibitors to the subject imparts an overall survival that is longer as compared to standard of care therapy available at the time of the present invention.
In various embodiments, the subject's pancreatic cancer has progressed on at least one line of therapy for metastatic disease or wherein the subject is intolerant of at least one line of therapy for metastatic disease. In various embodiments, the subject's pancreatic cancer has recurred with metastatic disease less than or equal to 12 weeks of completion of neoadjuvant or adjuvant systemic chemotherapy. In various embodiments, wherein the subject has locally advanced pancreatic cancer whose pancreatic cancer progressed to metastatic disease less than or equal to 12 weeks after completion of systemic chemotherapy. In various embodiments, the subject's pancreatic cancer has recurred with metastatic disease less than or equal to 12 weeks of completion of systemic chemotherapy.
In various embodiments, the pancreatic cancer is adenocarcinoma, acinar cell carcinoma, mixed acinar/neuroendocrine carcinoma, or solid pseudopapillary neoplasm.
In various embodiments, the mutation is in BRAF. In various embodiments, the mutation is selected from: (a) BRAF V600E, (b) oncogenic fusion of BRAF and another gene, (c) non-V600 mutation, insertion or deletion, or a mutation other than (a)-(c) with an additional driver mutation.
In various embodiments, the mutation is BRAF V600E. In various embodiments, the mutation is BRAF N486_P490del.
In various embodiments, the mutation is on exon 15. In various embodiments, the mutation is V600E, V600_K601delinsE, T599dup, V600R, or V600E and Q609L.
In various embodiments, the mutation is on exon 11. In various embodiments, the mutation is G469A, K483E, L485F, or V487_P492delinsA.
In various embodiments, the mutation is a fusion mutation. In various embodiments, the mutation is SND1-BRAF Fusion, BRK1-RAF1 Fusion, DTNA-RAF1 Fusion, FGD5-RAF1 Fusion, GIPC2-BRAF Fusion, GLI2-BRAF Fusion, JHDM1D-BRAF Fusion; LUC7L2-BRAF Fusion, MKRN1-BRAF Fusion, TMEM9-BRAF Fusion, RAF1 Rearrangement, or BRAF Rearrangement.
In various embodiments, the mutation is T310I, D594G, G469S, G596D, G596R, N236K, S467L, T241P, X327_splice, ARAF S214A, RAF1 S259Y, T599K, V600 and a Confounding Driver, or N486_P490del and ROS1 Fusion.
In various embodiments, the subject has a mutation in BRAF, and the one or more MAPK pathway inhibitors is a BRAF inhibitor, a MEK inhibitor or both. In various embodiments, the subject has KRAS wild type.
In various embodiments, the one or more MAPK pathway inhibitors is a MEK inhibitor. In various embodiments, the MEK inhibitor is trametinib, cobimetinib, binimetinib, selumetinib, or mirdametinib. In various embodiments, the MEK inhibitor is Cobimetinib, Binimetinib, or Trametinib.
In various embodiments, the one or more MAPK pathway inhibitors is dabrafenib mesylate and trametinib dimethyl sulfoxide, cobimetinib fumarate, binimetinib, binimetinib and encorafenib, trametinib dimethyl sulfoxide, selumetinib sulfate, LNP-3794, dabrafenib mesylate, panitumumab and trametinib dimethyl sulfoxide, HL-085, mirdametinib, MK-2206 and selumetinib sulfate, trametinib dimethyl sulfoxide and uprosertib, ATI-450, ATR-002, CKI-27, CS-3006, durvalumab and selumetinib sulfate, E-6201, FCN-159, SHR-7390, TQB-3234, ABM-1383, ATR-004, ATR-005, ATR-006, EBI-1051, KZ-001, OTS-514, OTS-964, Small Molecule to Inhibit MKK4 for Acute Liver Failure and Chronic Liver Disease, Small Molecules to Inhibit Mitogen Activated Protein Kinase for Oncology, or SNR-1611.
In various embodiments, the one or more MAPK pathway inhibitors is a BRAF inhibitor. In various embodiments, the BRAF inhibitor is dabrafenib mesylate and trametinib dimethyl sulfoxide, sorafenib tosylate, binimetinib and encorafenib, dabrafenib mesylate, encorafenib, vemurafenib, sorafenib tosylate, dabrafenib mesylate, panitumumab and trametinib dimethyl sulfoxide, hydroxychloroquine and sorafenib tosylate, lifirafenib maleate, TAK-580, BAL-3833, belvarafenib, CKI-27, LUT-014, LXH-254, RXDX-105, TQB-3233, XP-102, UB-941, ABM-1310, AFX-1251, APL-102, ARI-4175, AZ-304, BGB-3245, INU-152, LYN-204, PV-103, REDX-05358, or SJP-1601. In various embodiments, the BRAF inhibitor is Dabrafenib, Vemurafenib, Encorafenib, Lifirafenib, belvarafenib, or sorafenib tosylate. In various embodiments, the BRAF inhibitor is Vemurafenib, Dabrafenib and Encorafenib.
In various embodiments, the one or more MAPK pathway inhibitors is Dabrafenib and trametinib, Vemurafenib and cobimetinib, Trametinib, or Vemurafenib. In various embodiments, the one or more MAPK pathway inhibitor is Binimetinib and Encorafenib. In various embodiments, the one or more MAPK pathway inhibitors is Vemurafenib and the method further comprises administering carboplatin and paclitaxel. In various embodiments, the one or more MAPK pathway inhibitors is Trametinib and the method further comprises administering Pembrolizumab.
In various embodiments, administering one or more MAPK pathway inhibitors to the subject comprises one or more cycles. In various embodiments, administering one or more MAPK pathway inhibitors to the subject comprises 2, 3, 4, 5, 6, 7, 8, 9, or 10 or more cycles. In various embodiments, each cycle is about 28 days. In various embodiments, each cycle is about 21 days. In various embodiments, each cycle is about 35 days. In various embodiments, each cycle is about 7, 14, 21, 28, 35, 42, 49 or 56 days.
In various embodiments, the method comprises administering up to 36 cycles. In other embodiments, the method comprises administering more than 36 cycles. In various embodiments, the method comprises administering 1-3 cycles, 4-6 cycles, 7-9 cycles, 10-12 cycles, 13-15 cycles, 16-18 cycles, 19-21 cycles, 22-24 cycles, 25-27 cycles, 28-30 cycles, 31-33 cycles, or 35-56 cycles. In various embodiments, the method comprises administering until disease progression.
In various embodiments cycle 2 is the same as cycle 1. In various embodiments, the doses for cycle 3 is less than cycle 1 or cycle 2. In various embodiments, the doses for cycle 3 and/or subsequent cycles is less than cycle 1 or cycle 2.
In various embodiments the doses can be reduced after one cycle. In various embodiments the doses can be reduced after two cycles. Reduction of doses can be due; for example, to adverse events as described herein.
In some embodiments of the invention, the dose of the one or more MAPK pathway inhibitors can each be in the range of about 10-50 μg/day, 50-100 μg/day, 100-150 μg/day, 150-200 μg/day, 100-200 μg/day, 200-300 μg/day, 300-400 μg/day, 400-500 μg/day, 500-600 μg/day, 600-700 μg/day, 700-800 μg/day, 800-900 μg/day, 900-1000 μg/day, 1000-1100 μg/day, 1100-1200 μg/day, 1200-1300 μg/day, 1300-1400 μg/day, 1400-1500 μg/day, 1500-1600 μg/day, 1600-1700 μg/day, 1700-1800 μg/day, 1800-1900 μg/day, 1900-2000 μg/day, 2000-2100m/day, 2100-2200 μg/day, 2200-2300 μg/day, 2300-2400 μg/day, 2400-2500 μg/day, 2500-2600 μg/day, 2600-2700m/day, 2700-2800 μg/day, 2800-2900m/day or 2900-3000 μg/day.
In various embodiments, the dose of the one or more MAPK pathway inhibitors can be can each be in the range of about 10-50 mg/day, 50-100 mg/day, 100-150 mg/day, 150-200 mg/day, 100-200 mg/day, 200-300 mg/day, 300-400 mg/day, 400-500 mg/day, 500-600 mg/day, 600-700 mg/day, 700-800 mg/day, 800-900 mg/day, 900-1000 mg/day, 1000-1100 mg/day, 1100-1200 mg/day, 1200-1300 mg/day, 1300-1400 mg/day, 1400-1500 mg/day, 1500-1600 mg/day, 1600-1700 mg/day, 1700-1800 mg/day, 1800-1900 mg/day, 1900-2000 mg/day, 2000-2100 mg/day, 2100-2200 mg/day, 2200-2300 mg/day, 2300-2400 mg/day, 2400-2500 mg/day, 2500-2600 mg/day, 2600-2700 mg/day, 2700-2800 mg/day, 2800-2900 mg/day or 2900-3000 mg/day.
In various embodiments, the method further comprises administering one or more chemotherapy drugs, one or more PD-1 inhibitors or PD-L1 inhibitors, both a chemotherapy drug and a PD-1 inhibitor, or both a chemotherapy drug and a PD-L1 inhibitor.
Examples of chemotherapeutic agents include cytotoxic agents (e.g., 5-fluorouracil, cisplatin, carboplatin, methotrexate, daunorubicin, doxorubicin (Adriamycin®), vincristine, vinblastine, oxorubicin, carmustine (BCNU), lomustine (CCNU), cytarabine USP, cyclophosphamide, estramucine phosphate sodium, altretamine, hydroxyurea, ifosfamide, procarbazine, mitomycin, busulfan, cyclophosphamide, mitoxantrone, carboplatin, cisplatin, interferon alfa-2a recombinant, paclitaxel, teniposide, and streptozoci), cytotoxic akylating agents (e.g., busulfan, chlorambucil, cyclophosphamide, melphalan, or ethylesulfonic acid), alkylating agents (e.g., asaley, AZQ, BCNU, busulfan, bisulphan, carboxyphthalatoplatinum, CBDCA, CCNU, CHIP, chlorambucil, chlorozotocin, cis-platinum, clomesone, cyanomorpholinodoxorubicin, cyclodisone, cyclophosphamide, dianhydrogalactitol, fluorodopan, hepsulfam, hycanthone, iphosphamide, melphalan, methyl CCNU, mitomycin C, mitozolamide, nitrogen mustard, PCNU, piperazine, piperazinedione, pipobroman, porfiromycin, spirohydantoin mustard, streptozotocin, teroxirone, tetraplatin, thiotepa, triethylenemelamine, uracil nitrogen mustard, and Yoshi-864), antimitotic agents (e.g., allocolchicine, Halichondrin M, colchicine, colchicine derivatives, dolastatin 10, maytansine, rhizoxin, paclitaxel derivatives, paclitaxel, thiocolchicine, trityl cysteine, vinblastine sulfate, and vincristine sulfate), plant alkaloids (e.g., actinomycin D, bleomycin, L-asparaginase, idarubicin, vinblastine sulfate, vincristine sulfate, mitramycin, mitomycin, daunorubicin, VP-16-213, VM-26, navelbine and taxotere), biologicals (e.g., alpha interferon, BCG, G-CSF, GM-CSF, and interleukin-2), topoisomerase I inhibitors (e.g., camptothecin, camptothecin derivatives, and morpholinodoxorubicin), topoisomerase II inhibitors (e.g., mitoxantron, amonafide, m-AMSA, anthrapyrazole derivatives, pyrazoloacridine, bisantrene HCL, daunorubicin, deoxydoxorubicin, menogaril, N,N-dibenzyl daunomycin, oxanthrazole, rubidazone, VM-26 and VP-16), and synthetics (e.g., hydroxyurea, procarbazine, o,p′-DDD, dacarbazine, CCNU, BCNU, cis-diamminedichloroplatimun, mitoxantrone, CBDCA, levamisole, hexamethylmelamine, all-trans retinoic acid, gliadel and porfimer sodium).
Examples of anti-PD1 inhibitor that can be used in the methods described herein can be selected from the group consisting of pembrolizumab, nivolumab, pidilizumab, AMP-224, AMP-514, spartalizumab, cemiplimab, AK105, BCD-100, BI 754091, JS001, LZMO09, MGA012, Sym021, TSR-042, MGD013, AK104, XmAb20717, tislelizumab, PF-06801591, anti-PD1 antibody expressing pluripotent killer T lymphocytes (PIK-PD-1), autologous anti-EGFRvIII 4SCAR-IgT cells, and combinations thereof. Examples of anti-PDL1 inhibitor that can be used in the methods described herein can be selected from the group consisting of BGB-A333, CK-301, FAZ053, KN035, MDX-1105, MSB2311, SHR-1316, atezolizumab, avelumab, durvalumab, BMS-936559, CK-301, M7824, and combinations thereof.
In various embodiments, the method of treating pancreatic cancer in a subject in need thereof, comprising administering one or more MAPK pathway inhibitors to the subject, wherein the subject has a mutation in one or more genes in the MAPK signaling pathway, wherein the one or more MAPK pathway inhibitor is Binimetinib and Encorafenib, wherein the mutation is BRAF V600E, and the pancreatic cancer is BRAF V600E mutated pancreatic ductal adenocarcinoma (PDAC). In various embodiments, the subject's pancreatic cancer has progressed on at least one line of therapy for metastatic disease or wherein the subject is intolerant of at least one line of therapy for metastatic disease. In various embodiments, the subject's pancreatic cancer has recurred with metastatic disease less than or equal to 12 weeks of completion of neoadjuvant or adjuvant systemic chemotherapy. In various embodiments, wherein the subject has locally advanced pancreatic cancer whose pancreatic cancer progressed to metastatic disease less than or equal to 12 weeks after completion of systemic chemotherapy. In various embodiments, the subject's pancreatic cancer has recurred with metastatic disease less than or equal to 12 weeks of completion of systemic chemotherapy.
In various embodiments, administering one or more MAPK pathway inhibitors to the subject comprises one or more cycles. In various embodiments, administering one or more MAPK pathway inhibitors to the subject comprises 2, 3, 4, 5, 6, 7, 8, 9, or 10 or more cycles. In various embodiments, each cycle is about 28 days. In various embodiments, each cycle is about 21 days. In various embodiments, each cycle is about 35 days. In various embodiments, each cycle is about 7, 14, 21, 28, 35, 42, 49 or 56 days.
In various embodiments, the method comprises administering up to 36 cycles. In other embodiments, the method comprises administering more than 36 cycles. In various embodiments, the method comprises administering 1-3 cycles, 4-6 cycles, 7-9 cycles, 10-12 cycles, 13-15 cycles, 16-18 cycles, 19-21 cycles, 22-24 cycles, 25-27 cycles, 28-30 cycles, 31-33 cycles, or 35-56 cycles. In various embodiments, the method comprises administering until disease progression.
In various embodiments, each cycle comprises administering about 150-525 mg of encorafenib orally daily and about 7.5-52.5 mg of binimetinib orally twice daily; for example, for 28 days.
In various embodiments, each cycle comprises administering about 225-450 mg of encorafenib orally daily and about 15-45 mg of binimetinib orally twice daily; for example, for 28 days.
In various embodiments, each cycle comprises administering about 450 mg of encorafenib orally daily and about 45 mg of binimetinib orally twice daily; for example, for 28 days. In various embodiments, each cycle comprises administering about 300 mg of encorafenib orally daily and about 30 mg of binimetinib orally twice daily; for example, for 28 days. In various embodiments, each cycle comprises administering about 225 mg of encorafenib orally daily and about 15 mg of binimetinib orally twice daily; for example, for 28 days.
In various embodiments, cycle 1 comprises administering about 450 mg of encorafenib orally daily and about 45 mg of binimetinib orally twice daily. In various embodiments cycle 2 is the same as cycle 1. In various embodiments, the doses for cycle 3 is less than cycle 1 or cycle 2. In various embodiments, the doses for cycle 3 and/or subsequent cycles is less than cycle 1 or cycle 2.
In various embodiments, cycle 1 comprises administering about 300 mg of encorafenib orally daily and about 30 mg of binimetinib orally twice daily. In various embodiments cycle 2 is the same as cycle 1.
In various embodiments, cycle 1 comprises administering about 225 mg of encorafenib orally daily and about 15 mg of binimetinib orally twice daily. In various embodiments cycle 2 is the same as cycle 1.
In various embodiments the doses can be reduced after one cycle. In various embodiments the doses can be reduced after two cycles. Reduction of doses can be due; for example, to adverse events as described herein.
Reduced doses can comprise; for example, administering about 300 mg of encorafenib orally daily and about 30 mg of binimetinib orally twice daily; or administering about 225 mg of encorafenib orally daily and about 15 mg of binimetinib orally twice daily.
In various embodiments, doses of encorafenib is not re-escalated after the dose reduction; for example, due to prolonged ATcF ≥about 501 msec.
In various embodiments, doses of binimetinib is not allowed to be re-escalated; for example, after a dose reduction due to LVEF dysfunction.
In various embodiments, doses of binimetinib or encorafenib is not allowed to be re-escalated; for example, after a dose reduction due to retinal toxicity ≥Grade 2.
In various embodiments, if binimetinib is withheld, encorafenib can be reduced to a maximum dose of 300 mg daily until binimetinib is resumed.
As used herein, the term “administering,” refers to the placement an agent as disclosed herein into a subject by a method or route which results in at least partial localization of the agents at a desired site. “Route of administration” may refer to any administration pathway known in the art, including but not limited to aerosol, nasal, via inhalation, oral, anal, intra-anal, pen-anal, transmucosal, transdermal, parenteral, enteral, topical or local. “Parenteral” refers to a route of administration that is generally associated with injection, including intratumoral, intracranial, intraventricular, intrathecal, epidural, intradural, intraorbital, infusion, intracapsular, intracardiac, intradermal, intramuscular, intraperitoneal, intrapulmonary, intraspinal, intrastemal, intrathecal, intrauterine, intravascular, intravenous, intraarterial, subarachnoid, subcapsular, subcutaneous, transmucosal, or transtracheal. Via the parenteral route, the compositions may be in the form of solutions or suspensions for infusion or for injection, or as lyophilized powders. Via the enteral route, the pharmaceutical compositions may be in the form of tablets, gel capsules, sugar-coated tablets, syrups, suspensions, solutions, powders, granules, emulsions, microspheres or nanospheres or lipid vesicles or polymer vesicles allowing controlled release. Via the topical route, the pharmaceutical compositions may be in the form of aerosol, lotion, cream, gel, ointment, suspensions, solutions or emulsions. In accordance with the present disclosure, “administering” may be self-administering. For example, it is considered as “administering” that a subject consumes a composition as disclosed herein.
The following examples are provided to better illustrate the claimed invention and are not to be interpreted as limiting the scope of the invention. To the extent that specific materials are mentioned, it is merely for purposes of illustration and is not intended to limit the invention. One skilled in the art may develop equivalent means or reactants without the exercise of inventive capacity and without departing from the scope of the invention.
BRAF Alteration Frequency in a Real-World Cohort with Genomic Testing Results
To assess the frequency of BRAF alterations in PC, real-world data were obtained via the Perthera Platform which includes 1802 patients who underwent molecular profiling as part of the Know Your Tumor Program (KYT) and other hospital programs. Additional public data were obtained from 1979 patients with genomic testing results available via the AACR GENIE project (release 6.1.0). Genomic profiling data from this aggregated cohort of 3781 patients with pancreatic cancer were analyzed to assess the prevalence of BRAF alterations (see the “Prevalence Cohort” described in Table 1). Molecular profiles with fewer than 3 genomic variants detected were removed from the aggregated prevalence cohort to exclude low quality genomic testing results. Given the deidentified nature of these two data sets, the possibility of subjects with duplicative entries cannot be entirely ruled out; however, spot checking for highly similar variant profiles across databases suggested minimal overlap (data not shown). Histologic subtypes included in our case series were epithelial pancreatic cancers including ductal adenocarcinoma, acinar cell carcinoma, solid pseudopapillary neoplasm, and pancreaticoblastoma. Tumors with predominantly neuroendocrine features were excluded from the clinical case series cohort.
Case Series of BRAF-Mutated Pancreatic Cancer from KYT and Academic Collaborators
De-identified patient and genomic information was collected by collaborators from Dana-Farber, MD Anderson Cancer Center (MDACC), Memorial Sloan Kettering (MSK), PanCAN, Inova Schar Cancer Institute (ISCI) and Cedars-Sinai Medical Center (CSMC).
Individual patient charts were retrospectively reviewed, and clinical information was extracted.
PanCAN and Perthera initiated an IRB-approved observational registry trial to capture real-world outcomes across all lines of therapies and NGS testing results from CLIA-certified commercial laboratories in addition to proteomics/phosphoproteomics data as previously described [PMID: 32135080]. At MSK, DNA testing through MSK-IMPACT Assay (Memorial Sloan Kettering-Integrated Mutation Profiling of Actionable Cancer Targets) was used to identify the somatic genomic mutation profile of treated patients. BRAF somatic mutations in PC patients were identified using the cBioPortal. At MDACC samples were identified using the Molecular and Clinical Data Integration Platform (MOCLIP) of the Khalifa Institute for Personalized Cancer Therapy. Somatic DNA sequencing at Dana-Farber/Brigham and Women's Cancer Center was accomplished with an institutionally supported, CLIA-certified, hybrid-capture and massively parallel sequencing assay (OncoPanel) for adult and pediatric patients with cancer. Tumor biopsy samples from the ISCI and CSMC were sent to a CLIA-certified, CAP-accredited commercial laboratory (Foundation Medicine).
The investigators established the hypotheses and data points of interest after a series of scheduled meetings. Demographic, clinical descriptors, and outcomes data were collected under institution-specific IRB-approved protocols for each individual site. The data from each site was de-identified before being shared in a HIPAA-compliant database. A total of 81 patients with BRAF-mutated PC were identified from these institutions. however, population-level frequencies of BRAF alterations in PC could not be assessed since the denominator (i.e. the number of patients with adequate molecular profiling data) was not available outside the PanCAN/Perthera database. This motivated the need to combine public data with the PanCAN/Perthera dataset (as described above) to establish a Prevalence Cohort suitable for frequency analyses. Notably, this retrospective case series reflects a population of patients being treated predominantly at academic medical centers across coastal regions of the United States. Thus, this case series may not adequately represent important population-level factors (e.g. differences in insurance coverage, socioeconomic status, urban vs rural cohorts, and academic vs community settings) that can influence patient outcomes as well as access to targeted therapies either off label or on a clinical trial.
Demographic data, date of diagnosis, staging information, treatment history, and response to therapy were collected from all participating sites. Overall survival (OS) was calculated from the time of the patient's diagnosis of advanced PC (i.e. the date of recurrence following surgical resection or the date of initial diagnosis in the context of metastatic or unresectable disease) until death (survival event) or last follow-up (censored event). All OS survival analyses excluded patients who did not receive any therapy in the advanced setting. Progression-free survival (PFS) was calculated from the time of initiation of a therapy until discontinuation due to disease progression (survival event), cessation due to tolerability issues (censored event), or the last follow-up (censored event).
All survival analysis methods were implemented in an R/Bioconductor programming environment. All PFS analyses excluded patients with early stage or resectable disease. All OS analyses included only the subset of patients who were diagnosed with metastatic (Stage IV) disease at initial presentation. Patient outcomes related to PFS and OS were assessed using Cox proportional hazards regression models from the survival package and visualized using the survminer package. Multivariate Cox regression models were utilized to assess potentially confounding factors (e.g. 1st line of therapy versus later lines of therapy for PFS analyeses; adenocarcinoma versus acinar cell carcinoma histology) when appropriate (see
In a real-world cohort of 3781 PC patients with genomic testing results available, we identified 84 patients (2.2% of 3781 with PC) whose tumors harbored BRAF alterations within this “Prevalence Cohort” (Table 1).
We categorized each patient's molecular profile that included a BRAF alteration within the prevalence cohort into one of four subgroups: 26/84 (31%) Exon 15 (V600) mutations, 21/84 (25%) Exon 11 (non-V600) mutations/insertions/deletions, 20/84 (24%) oncogenic fusions (Fusions), or 17/84 (20%) non-canonical profiles (Other) with either multiple oncogenic drivers or atypical BRAF variants (Table 1). Although BRAF mutations are often mutually exclusive of mutations in other driver genes, we identified confounding genomic alterations (e.g. a KRAS/HRAS activating mutation or a ROS1 activating fusion event) within the molecular profiles of four patients with mutations that might otherwise have been assigned to the Exon 15 (10.3%, 3/29) or Exon 11 (4.5%, 1/22) subgroups. For those without any confounding drivers, the most common BRAF alterations included the canonical V600E mutation in Exon 15 (17/84, 20.2%), a recurring five-amino-acid in-frame deletion in the BRAF β3-αC loop within Exon 11 commonly referred to as ΔNVTAP or N486_P490del (16/84, 19.0%), and SND1-BRAF Fusions (9/84, 10.7%). These 3 specific BRAF alterations have distinct implications for targeted therapy (see Table 3) and form the basis of the Exon 15, Exon 11, and Fusion subgroups considered throughout this study.
Within the prevalence cohort, the proportion of BRAF alterations was higher (p=0.000000691, Fisher's Exact Test) in pancreatic acinar cell carcinoma (9/49, 18.4%) relative to pancreatic adenocarcinoma (64/3298, 1.9%). We observed that pancreatic acinar cell carcinoma frequently harbored BRAF fusion events (6/49, 12.2%) compared to mutation rates within pancreatic adenocarcinoma that are not expected to exceed 1% for any of the four BRAF mutational subgroups. BRAF Exon 15 (V600) mutations were also observed in rare PC histologies, with one in a pancreatoblastoma and in a solid pseudopapillary neoplasm.
In a retrospective case series of PC patients, with clinically annotated outcomes data, we identified 81 patients with genomic alterations in BRAF (
In this cohort, BRAF alterations occurring in adenocarcinoma subjects were evenly distributed between BRAF Exon 15 mutations (16/62), BRAF Exon 11 mutations (17/62), BRAF/RAF1 oncogenic fusion events (14/62) and the “Other” subgroup consisting of other genomic alterations, poorly-characterized variants, or genomic profiles with multiple drivers (14/62), In acinar cell tumors, the majority of alterations were fusion abnormalities (10/14) and Exon 15 mutations (V600) mutations (3/14). In the 4 subjects with IPMN, there was an Exon 15 V600 mutation, an Exon 11 in-frame deletion, and 2 missense mutations. One subject with a pancreaticoblastoma had a V600E alteration, which has not been reported previously.
In order to assess response to BRAF-directed therapy, genomic profiles from the clinical cohort were classified into one of four subgroupings (Table 1) as described above for the prevalence cohort: Exon 15 (V600) (17/81, 21.0%), Fusions (25/81, 30.9%), Exon 11 (non-V600) (18/81, 22.2%), or Other (21/81, 25.9%) which included BRAF mutations found alongside confounding oncogenic drivers or uncharacterized/atypical alterations that were not appropriate for assignment to the Exon 11, Exon 15, or Fusion subgroups (see Table 3 for additional details related to the rationale for each patient's subgrouping). Of the 17 BRAFV600E alterations, 14 were mutually exclusive of additional alterations in BRAF, KRAS, or other potential driving alterations. If a known confounding driver was identified alongside any BRAF alteration (including 3 profiles with BRAF V600E mutations), they were grouped in Other. Activating KRAS mutations were mutually exclusive with BRAF fusion events (0/25 co-occurrences) within the clinical cohort (as well as the prevalence cohort); however, one tumor with a BRAF V600E mutation also had a SND1-BRAF fusion. The canonical BRAF ΔNVTAP mutation, BRAF N486_P490del, was the most common variant (n=16) within the Exon 11 cohort (n=18). This BRAF N486_P490del variant was mutually exclusive with KRAS mutations (n=0 overlap) in the clinical cohort (note: n=1 overlap with a ROS1 fusion from the prevalence cohort).
Our last category, termed “Other”, included 21 patients with BRAF variants that were interpreted as pathogenic by the genomic testing laboratory but not considered appropriate for the Exon 11, Exon 15, or Fusion subgroups when taking into consideration the entire tumor genomic profile. known to be RAS-dependent (e.g. BRAF “class 3” variants do not share the same implications for therapy as “class 1” V600 variants), found alongside a confounding driver mutation (e.g. KRAS), or not otherwise classifiable into the Exon 11, Exon 15, or Fusion categories (see Table 3 for patient-specific assignment details for this category). In this grouping, 15/21 had multiple distinct drivers. Of those with multiple drivers outside of the BRAF gene, 13/15 had KRAS alterations, one NTRK fusion, and a RAF1 mutation. Of the three patients with 2 alterations in the BRAF gene, one had both a V600E and a SND1-BRAF fusion, whereas another had both a BRAF S467L mutation with a complex BRAF Exon 2-10 deletion (unclear functional impact; however, similar alterations have been associated with acquired resistance to BRAF inhibitors, but no targeted therapies were documented in this patient's history). Each of the 17 non-canonical BRAF alterations were unique in this clinical cohort (
We performed exploratory survival analyses to assess the prognostic impact of each BRAF subgrouping (
MEK and RAE Inhibitors have Activity in Patients WIth KRAS Wild Type and BRAT-Mutated Pancreatic Cancers
Response to BRAF targeted therapy was evaluated by the subgrouping described herein (
To better understand the possible real-world impact of BRAF-directed therapy on patient outcomes, we compared overall survival between patients with BRAF alterations (delineated by BRAF subclass) who received a molecularly-matched therapy targeting the MAPK signaling pathway (e.g. a BRAF, pan-RAF, MEK, or ERK inhibitor) vs patients with BRAF alterations who only received unmatched therapies (see Table 2 for baseline characteristics across the matched and unmatched cohorts). First, we assessed overall survival for each BRAF subclass individually (
As an exploratory analysis, we evaluated median PFS for standard chemotherapy regimens across the clinical cohort and within each BRAF subgroup (
We analyzed PFS outcomes in the 1st line setting (
In a follow-up analysis focusing on the Fusion cohort, we identified a significant difference in PFS (p=0.0051 (HR=0.1 [0.02-0.50]) between FOLFIRINOX (mPFS=8.9m [7.5-N/R], n=14, 1st line or later) and Gem/nab-P (mPFS=2.8m [1.9-N/R], n=12, 1st line or later) via univariate Cox regression (
The following inclusion criteria are for purposes of the trial described herein. Performance of the embodiments of the present invention does not limit subject to these inclusion criteria. Pre-registration—Inclusion Criteria:
The following registration criteria are for purposes of the trial described herein. Performance of the embodiments of the present invention does not limit subject to these registration criteria. Registration—Inclusion Criteria
The following registration criteria are for purposes of the trial described herein. Performance of the embodiments of the present invention does not necessarily exclude subjects having these registration criteria. Registration—Exclusion Criteria
1CT scans-chest, abdomen, and pelvis with IV and PO contrast; in the case of iodine contrast allergies an MRI of the abdomen and pelvis with gadolinium + a non-contrast chest CT is appropriate. Use same imaging throughout the study. Imaging at PD should only be repeated if not done within 28 days prior.
2For women of childbearing potential only. Must be done ≤ 7 days prior to registration.
3The diary must begin the day the patient starts taking the medication and must be completed per protocol and returned to the treating institution OR compliance must be documented in the medical record by any member of the care team.
4Streck tubes are required for this collection.
5 A full ophthalmic exam will be performed by an ophthalmologist at screening, as needed during on-study treatment phase and at end of treatment, and include best corrected visual acuity, slit lamp examination, intraocular pressure, dilated fundoscopy and Ocular Coherence Tomography (OCT). Examination of the retina is required, especially to identify findings associated with serous retinopathy and RVO.
6 After baseline, patients receiving binimetinib should be assessed at every physical examination for decreased visual acuity using a gross perimetry test (as opposed to automated visual field testing). Symptomatic patients should be referred for a full ophthalmic consultation.
7Results from a CLIA/CAP certified testing lab (commercial or institutional) that confirm the presence of a BRAFV600E mutation in the patient's tumor must be submitted for central review. See section 6.16 for detailed instructions.
9The 30 days after last dose of treatment evaluation can be done over the phone.
10 Scans are to be performed at screening, on Cycle 2 Day 1 and Cycle 5 Day 1, then every 12 weeks and end of treatment.
11CPK and Troponin levels to be completed Cycle 1 Day 1 and Cycle 2 Day 1.
Treatment Schedule—Starting Day 1 of Cycle 1 (28 day Cycles), patient will administer: Encorafenib 450 mg orally daily, Binimetinib 45 mg orally twice daily.
Strictly follow the modifications in this table for the first two cycles, until individual treatment tolerance can be ascertained. Thereafter, these modifications should be regarded as guidelines to produce mild-to-moderate, but not debilitating, side effects. If multiple adverse events are seen, administer dose based on greatest reduction required for any single adverse event observed. Reductions or increases apply to treatment given in the preceding cycle and are based on adverse events observed since the prior dose.
The lowest recommended dose level of encorafenib is 225 mg QD and the lowest recommended dose level of binimetinib is 15 mg BID. When the AE that resulted in a dose reduction improves to and remains stable at the patient's baseline level for a minimum of 14 days, the dose can be re-escalated to the next dose level at the discretion of the Investigator, provided there are no other concomitant toxicities that would prevent drug re-escalation. There is no limit to the number of times the patient can have their dose reduced or re-escalated, however:
Recommended Binimetinib Dose Modifications
Response and progression will be evaluated in this study using the new international criteria proposed by the revised Response Evaluation Criteria in Solid Tumors (RECIST) guidelines (version 1.1). Changes in the largest diameter (unidimensional measurement) of the tumor lesions and the short axis measurements in the case of lymph nodes are used in the RECIST guideline (Eisenhauer et al, 2009).
Schedule of Evaluations: For the purposes of this study, patients should be reevaluated every 8 weeks. In addition to a baseline scan, confirmatory scans should also be obtained not less than 4 weeks following initial documentation of objective response.
Measurable Disease—
Non-Measurable Disease—
Measurement Methods:
Patients who are CR, PR, or SD will continue treatment per protocol for a maximum of 36 cycles. After 36 cycles, patients will go to event monitoring. Patients who develop PD while receiving therapy will go to the event-monitoring phase. Patients who go off protocol treatment for reasons other than PD will go to the event-monitoring phase per Section 18.0. Patients who develop non-CNS PD at any time should go to event monitoring. These patients should be treated with alternative chemotherapy if their clinical status is good enough to allow further therapy. Event monitoring is every 3 months (±14 days) for 5 years after registration. If the patient is still alive 5 years after registration, no further follow up is required.
A patient is deemed ineligible if after registration, it is determined that at the time of registration, the patient did not satisfy each and every eligibility criteria for study entry. The patient may continue treatment off-protocol at the discretion of the physician as long as there are no safety concerns, and the patient was properly registered. The patient will go directly to the event-monitoring phase of the study (or off study, if applicable). If the patient received treatment, all data up until the point of confirmation of ineligibility must be submitted. Event monitoring will be required.
A patient is deemed a major violation if protocol requirements regarding treatment in cycle 1 of the initial therapy are severely violated that evaluability for primary end point is questionable. All data up until the point of confirmation of a major violation must be submitted. The patient will go directly to the event-monitoring phase of the study. The patient may continue treatment off-protocol at the discretion of the physician as long as there are no safety concerns, and the patient was properly registered. Event monitoring will be required per Section 18.0 of the protocol.
A patient is deemed a cancel if he/she is removed from the study for any reason before any study treatment is given.
Background: Binimetinib is an orally bioavailable, selective and potent MEK1 and MEK 2 inhibitor. As a MEK inhibitor, this compound has the potential to benefit patients with advanced cancers by inhibiting the MAPK (mitogen-activated protein kinases) pathway.
Formulation: Binimetinib drug product is supplied as film-coated tablets in a dose strength of 15 mg. The film coated-tablets consist of binimetinib, colloidal silicon dioxide/silica colloidal anhydrous; croscarmellose sodium; lactose monohydrate; magnesium stearate; microcrystalline cellulose/cellulose, microcrystalline; and a commercial film coating. The tablet is ovaloid biconvex (capsule shaped), yellow to dark yellow in color. Binimetinib tablets can be constituted in 3:1 (v/v) Ora Sweet®/water at 1 mg/mL binimetinib concentration to provide an easy to swallow oral suspension.
Preparation and storage: Binimetinib film-coated tablets should not be stored above 25° C. and should be protected from light. Tablets are packaged in plastic bottles acceptable for pharmaceutical use.
Administration: Binimetinib is administered twice daily with water, approximately 12 hours apart with or without meals. Tablets should be swallowed whole and should not be chewed.
Pharmacokinetic information—Absorption: The pharmacokinetics of binimetinib are characterized by moderate to high variability, accumulation of approximately 1.5-fold, and steady state concentrations reached within 15 days. The human ADME study CMEK162A2102 indicated that approximately 50% of binimetinib dose was absorbed. Distribution: Binimetinib is more distributed in plasma than blood. The blood-to-plasma concentration ratio of binimetinib in humans is 0.718. It is highly bound to plasma proteins (humans: 97.2%). Metabolism: The primary metabolic pathways include glucuronidation (up to 61.2% via UGT1A1), N-dealkylation (up to 17.8% via CYP1A2 and CYP2C19) and amide hydrolysis. Excretion: The excretion route was 31.7% of unchanged binimetinib in feces and 18.4% in urine. Estimated renal clearance of unchanged binimetinib was 6.3% of total dose.
Potential Drug Interactions: Overall, the risk for binimetinib to be a cause of or be affected by significant drug-drug interactions is predicted to be low. However, given the predominant role of UGT1A1 in the metabolism of binimetinib, and because the effect of a UGT1A1 inhibitor or inducer has not been evaluated in a formal clinical study, special consideration should be taken for co-administration of drugs that are UGT1A1 inhibitors or inducers, and administration of binimetinib to patients with low UGT1A1 activity. Binimetinib has been shown to be a substrate for P-gp and BCRP in vitro. The impact of P-gp/BCRP inhibitors on the PK of binimetinib in vivo is unknown; therefore, it is recommended that P-gp and BCRP inhibitors are dosed with caution.
Known potential toxicities—Very Common (≥10%)—diarrhea, nausea, vomiting, fatigue, peripheral edema, increased AST, increased blood creatine phosphokinase, dermatitis acneiform, dry skin, pruritus, rash, decreased ejection fraction. Common (≥1%-<10%)—chorioretinopathy, dry eye, macular edema, retinal detachment, retinal vein occlusion, retinopathy, serous retinal damage, blurred vision, reduced visual acuity, visual impairment, abdominal pain, constipation, dyspepsia, gastroesophageal reflux disease, asthenia, facial edema, edema, malaise, pyrexia, folliculitis, paronychia, pustular rash, increased ALT, increased amylase, increased blood alkaline phosphatase, increased blood creatinine, increased GGT, lipase increased, arthralgia, muscular weakness, myalgia, dizziness, dysgeusia, epistaxis, alopecia, xerosis, nail disorder, palmar-plantar erythrodysesthesia syndrome, eczema, erythema, erythematous rash, papular rash, macular rash, maculo-papular rash, skin fissures, hypertension, neutropenia, a pneumonitis. Uncommon (≥0.1-<1%)— anemia, left ventricular dysfunction, eye edema, gastritis, gastrointestinal hemorrhage, colitis, general physical health deterioration, infection, skin infection, cellulitis, erysipelas, irregular heart rate, hypoglycemia, musculoskeletal pain, rhabdomyolysis, dropped head syndrome, ageusia, pulmonary embolism, xeroderma, follicular rash, pruritic rash, deep vein thrombosis, hypertensive crisis, hypotension.
Background: Encorafenib is a potent and selective ATP-competitive inhibitor of BRAF V600-mutant kinase.
Formulation: The encorafenib drug product is supplied as a hard gelatin capsule in dosage strengths of 75 mg. The dosage forms for each strength have identical formulations which are packaged in different colored capsules: 75 mg capsule (FMI): Size #00 hard gelatin capsules; flesh opaque cap and white opaque body, with the markings “NVR” or stylized “A” on the cap and “LGX 75 mg” on body.
The capsules consist of encorafenib drug substance, copovidone, poloxamer 188, succinic acid, microcrystalline cellulose, colloid silicon dioxide, crospovidone, and magnesium stearate of vegetable origin.
Preparation and storage: Encorafenib hard gelatin capsules should not be stored above 25° C. and should be protected from moisture. Capsules are packaged in plastic bottles acceptable for pharmaceutical use and should not be repackaged at the site.
Administration: Encorafenib capsules are intended for oral administration with water; capsules should be swallowed whole and should not be chewed. Encorafenib capsules may also be opened and the powder mixed with sweetened applesauce; the soft food preparation is intended for oral administration with water. Encorafenib can be administered without regard to food.
Pharmacokinetic information—Absorption: At least 86% of the dose is absorbed. Metabolism: N-dealkylation is the primary metabolic pathway; CYP3A4 (primary), CYP2C19, and CYP2D6 (minor) contribute to total oxidative clearance in human liver microsomes. Half-life elimination: 6.32 hours (range 3.74 to 8.09 hours). Excretion: Feces (39%); urine (47.2%)
Potential Drug Interactions: Since encorafenib is mainly metabolized by CYP3A, the co-administration of CYP3A inducers might decrease the exposure of encorafenib in clinical practice. Thus, long term co-administration of strong and moderate inducers of CYP3A with encorafenib should be avoided. Clinical results from a dedicated DDI study with encorafenib and CYP3A inhibitors indicated concomitant administration of encorafenib with strong or moderate CYP3A inhibitors may increase encorafenib plasma concentration. If co-administration with strong or moderate CYP3A inhibitors cannot be avoided, dose reduction of encorafenib may be warranted. Since no clinical data are available, caution should be used for co-administering substrates of CYP3A4 and UGT1A1. Based on in vitro transporter studies, encorafenib can potentially inhibit the transporters P-gp, BCRP, OATP1B1, OATP1B3, OAT1, OAT3 and OCT2 at clinical concentrations. Co-administration of encorafenib with drugs that are substrates for these enzymes and/or transporters may alter the exposure of the co-administered medication.
Known potential toxicities—Very Common (≥10%): Gastrointestinal: diarrhea; Metabolism and nutrition: decreased appetite; Nervous system: peripheral neuropathy; Psychiatric: insomnia; Skin and subcutaneous: hair loss, dry skin, hyperkeratosis, pruritis, Palmar-plantar erythrodysesthesia syndrome, palmoplantar keratoderma, erythema; Vascular: flushing. Common (≥1%-<10%): Blood and lymphatic: anemia; Cardiac: tachycardia; Ear and labyrinth: vertigo; Eye: iridocyclitis; Gastrointestinal: nausea, vomiting, abdominal pain, constipation, dyspepsia, stomatitis; General: asthenia, fatigue, pyrexia, xerosis, chills, face edema, peripheral edema; Immune system: hypersensitivity; Investigations: AST and ALT increased, blood alkaline phosphatase increased, blood creatinine increased, gamma-glutamyltransferase increased, amylase increased, lipase increased, electrocardiogram QT prolonged; Metabolism and nutrition: dehydration, hyponatremia; Musculoskeletal and connective tissue: arthralgia, musculoskeletal pain, myalgia, muscle spasms, muscular weakness; Neoplasms benign, malignant and unspecified: keratoacanthoma, melanocytic nevus, skin papilloma, squamous cell carcinoma, dysplatic nevus, malignant melanoma; Nervous system: facial paralysis, facial paresis, ageusia, dysgeusia, dysesthesia, hyperesthesia, neuralgia; Renal and urinary: acute kidney injury, renal failure; Skin and subcutaneous: rash, photosensitivity reaction, skin exfoliation, skin hyperpigmentation. Uncommon (≥0.1%-<1%)—Eye: uveitis; Gastrointestinal: pancreatitis; Metabolism and nutrition: hyperglycemia; Musculoskeletal and connective tissue: back pain, pain in extremity; Neoplasms benign, malignant and unspecified: acanthoma, basal cell carcinoma; Nervous system: hypoaesthesia; Skin and subcutaneous: drug eruption, urticarial.
Various embodiments of the invention are described above in the Detailed Description. While these descriptions directly describe the above embodiments, it is understood that those skilled in the art may conceive modifications and/or variations to the specific embodiments shown and described herein. Any such modifications or variations that fall within the purview of this description are intended to be included therein as well. Unless specifically noted, it is the intention of the inventors that the words and phrases in the specification and claims be given the ordinary and accustomed meanings to those of ordinary skill in the applicable art(s).
The foregoing description of various embodiments of the invention known to the applicant at this time of filing the application has been presented and is intended for the purposes of illustration and description. The present description is not intended to be exhaustive nor limit the invention to the precise form disclosed and many modifications and variations are possible in the light of the above teachings. The embodiments described serve to explain the principles of the invention and its practical application and to enable others skilled in the art to utilize the invention in various embodiments and with various modifications as are suited to the particular use contemplated. Therefore, it is intended that the invention not be limited to the particular embodiments disclosed for carrying out the invention.
While particular embodiments of the present invention have been shown and described, it will be obvious to those skilled in the art that, based upon the teachings herein, changes and modifications may be made without departing from this invention and its broader aspects and, therefore, the appended claims are to encompass within their scope all such changes and modifications as are within the true spirit and scope of this invention. It will be understood by those within the art that, in general, terms used herein are generally intended as “open” terms (e.g., the term “including” should be interpreted as “including but not limited to,” the term “having” should be interpreted as “having at least,” the term “includes” should be interpreted as “includes but is not limited to,” etc.).
As used herein the term “comprising” or “comprises” is used in reference to compositions, methods, and respective component(s) thereof, that are useful to an embodiment, yet open to the inclusion of unspecified elements, whether useful or not. It will be understood by those within the art that, in general, terms used herein are generally intended as “open” terms (e.g., the term “including” should be interpreted as “including but not limited to,” the term “having” should be interpreted as “having at least,” the term “includes” should be interpreted as “includes but is not limited to,” etc.). Although the open-ended term “comprising,” as a synonym of terms such as including, containing, or having, is used herein to describe and claim the invention, the present invention, or embodiments thereof, may alternatively be described using alternative terms such as “consisting of” or “consisting essentially of.”
This application includes a claim of priority under 35 U.S.C. § 119(e) to U.S. provisional patent application No. 62/972,345, filed Feb. 10, 2020, the entirety of which is hereby incorporated by reference.
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/US2021/017286 | 2/9/2021 | WO |
| Number | Date | Country | |
|---|---|---|---|
| 62972345 | Feb 2020 | US |
