BRIEF DESCRIPTION OF THE DRAWINGS
Further features and advantages of the present invention will become apparent from the following detailed description, taken in combination with the appended drawings, in which:
FIG. 1 is a schematic drawing of an optical oxidative stress measurement device having a 50 μL sample cell with a 1 cm path length supplied with light from a broadband Tungsten Halogen lamp via a first optical fiber, a short wavelength near infrared (SWNIR) spectrophotometer coupled to an opposite end of the sample cell via a second optical fiber for detecting CW intensity in the 600 nm to 1100 nm range, and a computer connected to the spectrophotometer for recording and analyzing the spectra.
FIG. 2 is graph showing absorption levels from a variety of molecular species in the 600 to 1000 nm wavelength range.
FIG. 3 shows a Raman spectra of 20 IVF embryo culture media samples.
FIG. 4 shows mean Raman spectra with Standard Deviation.
FIG. 5 shows spectra for viable samples.
FIG. 6 shows cross validation.
FIG. 7 is a graph of metabolomic signatures of the oxidative stress constituents in the developing embryo obtained using capillary electrophoresis with ultraviolet optical detection which identifies nutrient and metabolite fractions of the media.
Patent Application
20070160973
