Claims
- 1. A DNA construct comprising as operably linked components in the direction of transcription, a promoter region obtainable from a gene selected from the group consisting of a napin gene, an EA9 gene or an acyl carrier protein gene; a DNA sequence of interest other than the native coding sequence of said gene; and a transcription termination region, wherein said components are functional in a plant cell, and wherein said DNA construct is flanked by T-DNA.
- 2. The DNA construct according to claim 1, wherein said DNA sequence of interest encodes an enzyme.
- 3. The DNA construct according to claim 1, wherein said DNA sequence of interest is an antisense sequence.
- 4. A plant cell having an altered phenotype as a result of expression of a DNA construct according to claim 1.
- 5. The plant cell according to claim 4, wherein said DNA construct is flanked by T-DNA.
- 6. The plant cell according to claim 5 wherein said cell is one from the group consisting of a soybean cell and a rapeseed cell.
- 7. The plant cell according to claim 4, wherein said DNA sequence of interest encodes an enzyme.
- 8. The plant cell according to claim 4, wherein said DNA sequence of interest is an antisense sequence.
- 9. A plant comprising cells comprising a DNA construct according to any one of claims 1-3.
- 10. The plant according to claim 9, wherein said plant is dicotyledonous.
- 11. Seed obtained from a plant according to claim 10.
- 12. Seed having a DNA construct according to claim 1.
- 13. The seed according to claim 12, wherein said seed is an oil seed or a grain seed.
- 14. The seed according to claim 12, wherein said seed is from a dicotyledonous plant.
- 15. The seed according to claim 14, wherein said seed is from a plant of the genus Brassica.
- 16. The seed according to claim 14, wherein said dicotyledonous plant is selected from the group consisting of cotton, soybean, safflower and sunflower.
- 17. A method for obtaining a plant having a modified phenotype, said method comprising;
transforming a host plant cell with a DNA construct under genomic integration conditions, wherein said construct comprises as operably linked components in the direction of transcription, a promoter region obtainable from a gene, wherein transcription of said gene is regulated in plant seed tissue, a DNA sequence of interest other than the native coding sequence of said gene, and a transcription termination region, wherein said components are functional in a plant cell, whereby said DNA construct becomes integrated into a genome of said plant cell; regenerating a plant from said transformed plant cell, and growing said plant under conditions whereby said DNA sequence of interest is expressed and a plant having said modified phenotype is obtained.
- 18. A method for altering the phenotype of plant seed tissue as distinct from other plant tissue, said method comprising:
growing a plant, wherein said plant comprises cells containing a DNA construct integrated into their genome, said DNA construct comprising, in the 5′ to 3′ direction of transcription, a transcriptional initiation region from a gene, wherein transcription of said gene is regulated in a plant seed tissue, a DNA sequence of interest other than the coding sequence native to said transcriptional initiation region, and a transcriptional termination region, whereby said DNA sequence of interest is transcribed under transcriptional control of said transcriptional initiation region and a plant having an altered phenotype is obtained.
- 19. The method according to claim 17 or 18, wherein said DNA construct is flanked by T-DNA.
- 20. The method according to claim 19, wherein said plant is a soybean or rapeseed plant.
- 21. The method according to claim 17 or 18 wherein said DNA sequence of interest encodes an enzyme.
- 22. The method according to claim 17 or 18 wherein said DNA sequence of interest is an antisense sequence.
- 23. The method according to claim 17 or 18 wherein said gene is transcribed during seed embryogenesis.
- 24. The method according to claim 23 wherein said gene is transcribed from about day 7 to day 40 postanthesis.
- 25. The method according to claim 17 or 18 wherein said gene is transcribed during seed maturation.
- 26. The method according to claim 25 wherein said gene is transcribed from about day 11 to day 30 postanthesis.
- 27. The method according to claim 18, wherein said transcriptional initiation region further comprises a translational initiation region and said DNA sequence of interest is an open reading frame encoding an amino acid sequence.
- 28. A method for modifying the genotype of a plant to impart a desired characteristic to seed as distinct from other plant tissue, said method comprising:
transforming under genomic integration conditions, a host plant cell with a DNA construct comprising in the 5′ to 3′ direction of transcription, a transcriptional initiation region from a gene; wherein transcription of said gene is regulated in a plant seed tissue, a DNA sequence of interest other than the native coding sequence of said gene, and a transcriptional termination region, whereby said DNA construct becomes integrated into the genome of said plant cell; regenerating a plant from said transformed host cell; and growing said plant to produce seed having a modified genotype.
- 29. The method according to claim 28, wherein said DNA construct is flanked by T-DNA.
- 30. The method according to claim 28, wherein said plant is a Brassica plant.
- 31. The method according to claim 28, wherein said DNA sequence of interest encodes an enzyme.
- 32. The method according to claim 28, wherein said DNA sequence of interest is an antisense sequence.
- 33. The method according to claim 28, wherein said plant is a soybean plant.
- 34. A method for modifying transcription in seed tissue as distinct from other plant tissue, said method comprising:
growing a plant capable of developing seed tissue under conditions to produce seed, wherein said plant comprises cells containing a DNA construct integrated into their genome, said DNA construct comprising, in the 5′ to 3′ direction of transcription, a seed-specific transcriptional initiation region, a DNA sequence of interest other than the coding sequence native to said transcriptional initiation region, and a transcriptional termination region, whereby said DNA sequence of interest is transcribed under transcriptional control of said seed-specific transcription initiation region.
- 35. The method according to claim 34, wherein said DNA sequence of interest is an antisense sequence.
- 36. The method according to claim 34, wherein said plant is of the genus Brassica.
- 37. The method according to claim 34, wherein said transcriptional initiation region further comprises a translational initiation region and said DNA sequence of interest is an open reading frame encoding an amino acid sequence.
- 38. The method according to claim 34, wherein said plant is a soybean plant.
- 39. A method to selectively express a heterologous DNA sequence of interest in seed tissue as distinct from other plant tissue, said method comprising:
growing a plant capable of developing seed tissue under conditions to produce seed, wherein said plant comprises cells having a genomically integrated DNA construct comprising, as operably linked components in the 5′ to 3′ direction of transcription, a seed-specific transcriptional initiation region and a translational initiation region, a DNA sequence of interest other than the coding sequence native to said transcriptional initiation region, a transcriptional termination region downstream of said DNA sequence of interest, whereby said DNA sequence of interest is expressed under control of said seed-specific transcriptional and translational initiation region.
- 40. The method according to claim 39, wherein said plant is of the genus Brassica.
- 41. The method according to claim 39, wherein said plant is a soybean plant.
Parent Case Info
[0001] This application is a continuation of U.S. Ser. No. 08/484,941, filed Jun. 7, 1995, which is a continuation of U.S. Ser. No. 08/105,852, filed Aug. 10, 1993, pending; U.S. Ser. No. 08/105,852 is a continuation in part of Ser. No. 07/526,123, filed May 21, 1990, pending, which is a continuation of Ser. No. 07/267,865, filed Nov. 2, 1988, abandoned, which is a continuation of Ser. No. 06/692,605, filed Jan. 17, 1985, abandoned; U.S. Ser. No. 08/105,852, is also a continuation in part of Ser. No. 07/582,241, filed Sep. 14, 1990, abandoned, which is a continuation of Ser. No. 07/188,361, filed Apr. 29, 1988, abandoned, which is a continuation in part of Ser. No. 07/168,190, filed Mar. 15, 1988, abandoned, which is a continuation in part of Ser. No. 07/054,369, filed May 26, 1987, which issued on Jul. 24, 1990 as U.S. Pat. No. 4,943,674; U.S. Ser. No. 08/105,852 is also a continuation in part of U.S. Ser. No. 07/742,834, Aug. 8, 1991, which issued as U.S. Pat. No. 5,420,034 issued on May 30, 1995, which is a continuation in part of Ser. No. 07/550,804, filed Jul. 9, 1990, abandoned, which is a continuation in part of Ser. No. 07/147,781, filed Jan. 25, 1988, abandoned, which is a continuation in part of Ser. No. 07/078,538, filed Jul. 28, 1987, abandoned, which is a continuation in part of Ser. No. 06/891,529, filed Jul. 31, 1986, which is abandoned.
Continuations (7)
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May 1990 |
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Continuation in Parts (9)
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07168190 |
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07188361 |
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07054369 |
May 1987 |
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07168190 |
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07742834 |
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08105852 |
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07550804 |
Jul 1990 |
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07742834 |
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07147781 |
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07550804 |
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07078538 |
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Jul 1986 |
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Jul 1987 |
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