Methods for detecting Hepatitis C virus using polynucleotides specific for same

Description

TECHNICAL FIELD
The invention relates to materials and methodologies for managing the spread of non-A, non-B hepatitis virus (NANBV) infection. More specifically, it relates to an etiologic agent of non-A, non-B hepatitis (NANBH), hepatitis C virus (HCV), and to polynucleotides and analogs thereof, which are useful in assays for the detection of HCV in biological samples.
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CITED PATENTS
U.S. Pat. No. 4,341,761
U.S. Pat. No. 4,399,121
U.S. Pat. No. 4,427,783
U.S. Pat. No. 4,444,887
U.S. Pat. No. 4,466,917
U.S. Pat. No. 4,472,500
U.S. Pat. No. 4,491,632
U.S. Pat. No. 4,493,890
U.S. Pat. No. 4,683,202
U.S. Pat. No. 4,458,066
U.S. Pat. No. 4,868,105
BACKGROUND ART
Non-A, Non-B hepatitis (NANBH) is a transmissible disease or family of diseases that are believed to be viral-induced, and that are distinguishable from other forms of viral-associated liver diseases, including that caused by the known hepatitis viruses, i.e., hepatitis A virus (HAV), hepatitis B virus (HBV), and delta hepatitis virus (HDV), as well as the hepatitis induced by cytomegalovirus (CMV) or Epstein-Barr virus (EBV). NANBH was first identified in transfused individuals. Transmission from man to chimpanzee and serial passage in chimpanzees provided evidence that NANBH is due to a transmissible infectious agent or agents.
Epidemiologic evidence is suggestive that there may be three types of NANBH: the water-borne epidemic type; the blood or needle associated type; and the sporadically occurring (community acquired) type. However, the number of agents which may be the causative of NANBH are unknown.
There have been a number of candidate NANBV. See, for example the reviews by Prince (1983), Feinstone and Hoofnagle (1984), and Overby (1985, 1986, 1987) and the article by Iwarson (1987). However, there is no proof that any of these candidates represent the etiological agent of NANBH.
The demand for sensitive, specific methods for screening and identifying carriers of NANBV and NANBV contaminated blood or blood products is significant. Post-transfusion hepatitis (PTH) occurs in approximately 10% of transfused patients, and NANBH accounts for up to 90% of these cases. The major problem in this disease is the frequent progression to chronic liver damage (25-55%).
Patient care as well as the prevention of transmission of NANBH by blood and blood products or by close personal contact require reliable screening, diagnostic and prognostic tools to detect nucleic acids, antigens and antibodies related to NANBV.
Methods for detecting specific polynucleotides by hybridization assays are known in the art. See, for example, Matthews and Kricka (1988), Analytical Biochemistry 169:1; Landegren et al. (1988), Science 242:229; and Mittlin (1989), Clinical chem. 35:1819. U.S. Pat. No. 4,868,105, issued Sep. 9, 1989, and in EPO Publication No. 225,807 (published Jun. 16, 1987).
DISCLOSURE OF THE INVENTION
Methods for isolating and/or detecting specific polynucleotides by hybridization could not be used for screening for HCV until Applicants' discovery of HCV, which provides materials and methods for obtaining the viral genomic sequences, which are provided in U.S. Pat. No. 5,350,671, and infra. Accordingly, one aspect of the invention is an oligomer capable of hybridizing to an HCV sequence in an analyte polynucleotide strand, wherein the oligomer is comprised of an HCV targeting sequence complementary to at least 4 contiguous nucleotides of HCV cDNA shown in FIG. 1.
Another aspect of the invention is a process for detecting an HCV sequence in an analyte strand suspected of containing an HCV polynucleotide, wherein the HCV polynucleotide comprises a selected target region, said process comprising:
(a) providing an oligomer capable of hybridizing to an HCV sequence in an analyte polynucleotide strand, wherein the oligomer is comprised of an HCV targeting sequence complementary to at least 4 contiguous nucleotides of HCV cDNA shown in FIG. 1
(b) incubating the analyte strand with the oligomer of (a) which allow specific hybrid duplexes to form between the targeting sequence and the target sequence; and
(d) detecting hybrids formed between target region, if any, and the oligomer.
Yet another aspect of the invention is a method for preparing blood free of HCV comprising:
(a) providing analyte nucleic acids from a sample of blood suspected of containing an HCV target sequence;
(b) providing an oligomer capable of hybridizing to the HCV sequence in an analyte polynucleotide strand, if any, wherein the oligomer is comprised of an HCV targeting sequence complementary to a sequence of at least 8 nucleotides present in a conserved HCV nucleotide sequence in HCV RNA;
(c) reacting (a) with (b) under conditions which allow the formation of a polynucleotide duplex between the targeting sequence and the target sequence, if any;
(d) detecting a duplex formed in (c), if any; and
(e) saving the blood from which complexes were not detected in (d).

BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 shows the the compiled HCV cDNA sequence derived from the clone described herein and from the compiled HCV cDNA sequence presented in U.S. Ser. No. 07/456,637. The clones from which the sequence was derived are 5'-clone32, b114a, 18g, ag30a, CA205a, CA290a, CA216a, pi14a, CA167b, CA156e, CA84a, CA59a, K9-1 (also called k9-1),26j, 13i, 12f, 14i, 11b, 7f, 7e, 8h, 33c, 40b, 37b, 35, 36, 81, 32, 33b, 25c, 14c, 8f, 33f, 33g, 39c, 35f, 19g, 26g, 15e, b5a, 16jh, 6k, and p131jh. In the figure the three horizontal dashes above the sequence indicate the position of the putative initiator methionine codon. Also shown in the figure is the amino acid sequence of the putative polyprotein encoded in the HCV cDNA. Heterogeneities in cloned DNAs of HCV1 are indicated by the amino acids indicated above the putatively encoded sequence of the large ORF; the parentheses indicate that the heterogeneity was detected at or near to the 5'- or 3'- end of the HCV cDNA in the clone.
FIG. 2 shows the sequences of capture and label probes for the detection of HCV RNA in biological samples.
FIG. 3 shows schematic alignment of a flaviviral polyprotein and a putative HCV polyprotein encoded in the major ORF of the HCV genome. Also indicated in the figure are the possible functions of the flaviviral polypeptides cleaved from the flaviviral polyprotein. In addition, the relative placements of the HCV polypeptides, NANB.sub.5-1-1 and C100, with respect to the putative HCV polyprotein are indicated.
FIG. 4 shows the nucleotide sequence of HCV cDNA in clone CA156e, the amino acids encoded therein, and the sequences which overlap with CA84a.
FIG. 5 shows the double-stranded nucleotide sequence of the HCV cDNA insert in clone 81, and the putative amino acid sequence of the polypeptide encoded therein.
FIG. 6 shows the HCV cDNA sequence in clone 36, the segment which overlaps the NANBV cDNA of clone 81, and the polypeptide sequence encoded within clone 36.
FIG. 7 shows the HCV cDNA sequence in clone 37b, the segment which overlaps clone 35, and the polypeptide encoded therein.
FIG. 8 shows autoradiographs of the HCV cPCR assay on RNA derived from liver samples of chimpanzees with NANBH (FIG. 8A) and on Italian patients with NANBH (FIG. 8B).
FIGS. 9A and 9B are graphs showing the temporal relationship between the display of liver damage, the presence of HCV RNA, and the presence of anti-HCV antibodies for two chimpanzees with NANBH.
FIG. 10 shows the nucleotide sequence of HCV cDNA in clone CA84a, the amino acids encoded therein, and the sequences which overlap with clone CA59a.
FIG. 11 shows the HCV cDNA sequence in clone 40b, the segment which overlaps clone 37b, and the polypeptide encoded therein.
FIG. 12 is an autoradiograph showing the labeled amplified products of approximately 300, 30, and 3 CID of HCV genomes.
FIG. 13 shows the nucleotide sequence of HCV cDNA in clone CA216a, the amino acids encoded therein, and the overlap with clone CA167b.
FIG. 14 shows the nucleotide sequence of HCV cDNA in clone CA290a, the amino acids encoded therein, and the overlap with clone CA216a.
FIG. 15 shows the nucleotide sequence of HCV cDNA in clone ag30a and the overlap with clone CA290a.
FIG. 16 is an autoradiograph showing amplified products extended from primers derived from conserved regions of the HCV genome.
FIG. 17 shows the HCV cDNA sequence in clone 35, the segment which overlaps clone 36, and the polypeptide encoded therein.
FIG. 18 shows the consensus sequences for five different HCV isolates from Japan and the United States.
FIG. 19 is a set of probes useful for the detection of HCV RNA derived from the core region.
FIG. 20 correlates the probes in FIG. 19 with their corresponding complementary regions in the HCV genome.
FIG. 21 shows an autoradiograph of a Northern blot of RNA isolated from the liver of an HCV infected chimpanzee, probed with HCV cDNA of clone 81.
FIG. 22 shows an autoradiograph of HCV nucleic acid treated with RNase A or DNase I, and probed with HCV cDNA of clone 81.
FIG. 23 shows autoradiographs of filters containing isolated HCV nucleic acids, probed with .sup.32 P-labeled plus and minus strand DNA probes derived from HCV cDNA in clone 81. FIG. 23 consists of two panels, FIGS. 23a and 23b, which are reproductions of the filters probed with the plus and minus strands, respectively.

MODES FOR CARRYING OUT THE INVENTION
The term "hepatitis C virus" (HCV) has been reserved by workers in the field for an heretofore unknown etiologic agent of NANBH. The prototype isolate of HCV has been identified in U.S. Ser. No. 122,714 now abandoned (See also E.P.O. Publication No. 318,216). The term HCV also includes new isolates of the same viral species. As an extension of this terminology, the disease caused by HCV, formerly called blood-borne NANB hepatitis (BB-NANBH), is called hepatitis C. The terms NANBH and hepatitis C may be used interchangeably herein.
HCV is a viral species of which pathogenic strains cause BB-NANBH. There may also be attenuated strains or defective interfering particles derived therefrom. As shown infra, the HCV genome is comprised of RNA. It is known that RNA containing viruses have relatively high rates of spontaneous mutation, i.e., reportedly on the order of 10.sup.-3 to 10.sup.-4 per incorporated nucleotide (Fields & Knipe (1986)). Therefore, since heterogeneity and fluidity of genotype are inherent in RNA viruses, there are multiple strains/isolates, which may be virulent or avirulent, within the HCV species. The compositions and methods described herein, enable the propagation, identification, detection, and isolation of the various HCV strains or isolates.
Several different strains/isolates of HCV have been identified (See U.S. Pat. No. 5,350,671). One such strain or isolate, which is a prototype, is named CDC/HCV1 (also called HCV1). Information from one strain or isolate, such as a partial genomic sequence, is sufficient to allow those skilled in the art using standard techniques to isolate new strains/isolates and to identify whether such new strains/isolates are HCV. For example, several different strains/isolates are described infra. These strains, which were obtained from a number of human sera (and from different geographical areas), were isolated utilizing the information from the genomic sequence of HCV1.
Using the techniques described in U.S. Ser. No. 07/456,637 and infra, the genomic structure and the nucleotide sequence of HCV1 genomic RNA has been deduced. The genome appears to be single-stranded RNA containing .about.10,000 nucleotides. The genome is positive-stranded, and possesses a continuous, translational open reading frame (ORF) that encodes a polyprotein of about 3,000 amino acids. In the ORF, the structural protein(s) appear to be encoded in approximately the first quarter of the N-terminus region, with the majority of the polyprotein responsible for non-structural proteins. When compared with all known viral sequences, small but significant co-linear homologies are observed with the non-structural proteins of the flavivirus family, and with the pestiviruses (which are now also considered to be part of the Flavirus family).
A schematic alignment of possible regions of a flaviviral polyprotein (using Yellow Fever Virus as an example), and of a putative polyprotein encoded in the major ORF of the HCV genome, is shown in FIG. 3. In the figure the possible domains of the HCV polyprotein are indicated. The flavivirus polyprotein contains, from the amino terminus to the carboxy terminus, the nucleocapsid protein (C), the matrix protein (M), the envelope protein (E), and the non-structural proteins (NS) 1, 2 (a+b), 3, 4 (a+b), and 5. Based upon the putative amino acids encoded in the nucleotide sequence of HCV1, a small domain at the extreme N-terminus of the HCV polyprotein appears similar both in size and high content of basic residues to the nucleocapsid protein (C) found at the N-terminus of flaviviral polyproteins. The non-structural proteins 2, 3, 4, and 5 (NS2-5) of HCV and of yellow fever virus (YFV) appear to have counter parts of similar size and hydropathicity, although there is divergence of the amino acid sequences. However, the region of HCV which would correspond to the regions of YFV polyprotein which contains the M, E, and NS1 protein not only differs in sequence, but also appears to be quite different both in size and hydropathicity. Thus, while certain domains of the HCV genome may be referred to herein as, for example, NS1, or NS2, it should be borne in mind that these designations are speculative; there may be considerable differences between the HCV family and flaviviruses that have yet to be appreciated.
Different strains, isolates or subtypes of HCV are expected to contain variations at the amino acid and nucleic acids compared with HCV1. Many isolates are expected to show much (i.e., more than about 40%) homology in the total amino acid sequence compared with HCV1. However, it may also be found that there are other less homologous HCV isolates. These would be defined as HCV according to various criteria such as, for example, an ORF of approximately 9,000 nucleotides to approximately 12,000 nucleotides, encoding a polyprotein similar in size to that of HCV1, an encoded polyprotein of similar hydrophobic and/or antigenic character to that of HCV1, and the presence of co-linear peptide sequences that are conserved with HCV1. In addition, it is believed that the genome would be a positive-stranded RNA.
All HCV isolates encode at least one epitope which is immunologically identifiable (i.e., immunologically cross-reactive) with an epitope encoded in the HCV cDNAs described herein. Preferably the epitope is contained in an amino acid sequence described herein and is unique to HCV when compared to previously known pathogens. The uniqueness of the epitope may be determined by its immunological reactivity with anti-HCV antibodies and lack of immunological reactivity with antibodies to known pathogens.
HCV strains and isolates are evolutionarily related. Therefore, it is expected that the overall homology of the genomes at the nucleotide level may be about 40% or greater, probably will be about 50% or greater, probably about 60% or greater, and even more probably about 80% or greater; and in addition that there will be corresponding contiguous sequences of at least about 13 nucleotides. It should be noted, as shown infra, that there are variable and hypervariable regions within the HCV genome; therefore, the homology in these regions is expected to be significantly less than that in the overall genome. The correspondence between the putative HCV strain genomic sequence and, for example, the CDC/HCV1 cDNA sequence can be determined by techniques known in the art. For example, they can be determined by a direct comparison of the sequence information of the polynucleotide from the putative HCV, and the HCV cDNA sequence(s) described herein. They also can be determined by hybridization of the polynucleotides under conditions which form stable duplexes between homologous regions (for example, those which would be used prior to S.sub.1 digestion), followed by digestion with single stranded specific nuclease(s), followed by size determination of the digested fragments.
Because of the evolutionary relationship of the strains or isolates of HCV, putative HCV strains of isolates are identifiable by their homology at the polypeptide level. Generally, HCV strains or isolates are expected to be at least 40% homologous, more than about 50% homologous, probably more than about 70% homologous, and even more probably more than about 80% homologous, and some may even be more than about 90% homologous at the polypeptide level. The techniques for determining amino acid sequence homology are known in the art. For example, the amino acid sequence may be determined directly and compared to the sequences provided herein. Alternatively the nucleotide sequence of the genomic material of the putative HCV may be determined (usually via a cDNA intermediate), the putative amino acid sequence encoded therein can be determined, and the corresponding regions compared.
As used herein, a polynucleotide "derived from" a designated sequence refers to a polynucleotide sequence which is comprised of a sequence of approximately at least about 6 nucleotides, preferably at least about 8 nucleotides, more preferably at least about 10-12 nucleotides, and even more preferably at least about 15-20 nucleotides corresponding to a region of the designated nucleotide sequence. "Corresponding" means homologous to or complementary to the designated sequence. Preferably, the sequence of the region from which the polynucleotide is derived is homologous to or complementary to a sequence which is unique to an HCV genome. More preferably, the derived sequence is homologous or complementary to a sequence that is unique to all or to a majority of HCV isolates. Whether or not a sequence is unique to the HCV genome can be determined by techniques known to those of skill in the art. For example, the sequence can be compared to sequences in databanks, e.g., Genebank, to determine whether it is present in the uninfected host or other organisms. The sequence can also be compared to the known sequences of other viral agents, including those which are known to induce hepatitis, e.g., HAV, HBV, and HDV, and to members of the Flaviviridae. The correspondence or non-correspondence of the derived sequence to other sequences can also be determined by hybridization under the appropriate stringency conditions. Hybridization techniques for determining the complementarity of nucleic acid sequences are known in the art, and are discussed infra. See also, for example, Maniatis et al. (1982). In addition, mismatches of duplex polynucleotides formed by hybridization can be determined by known techniques, including for example, digestion with a nuclease such as S1 that specifically digests single-stranded areas in duplex polynucleotides. Regions from which typical DNA sequences may be "derived" include but are not limited to, for example, regions encoding specific epitopes, as well as non-transcribed and/or non-translated regions.
The derived polynucleotide is not necessarily physically derived from the nucleotide sequence shown, but may be generated in any manner, including for example, chemical synthesis or DNA replication or reverse transcription or transcription. In addition, combinations of regions corresponding to that of the designated sequence may be modified in ways known in the art to be consistent with an intended use.
The term "recombinant polynucleotide" as used herein intends a polynucleotide of genomic, cDNA, semisynthetic, or synthetic origin which, by virtue of its origin or manipulation: (1) is not associated with all or a portion of a polynucleotide with which it is associated in nature, (2) is linked to a polynucleotide other than that to which it is linked in nature, or (3) does not occur in nature.
The term "polynucleotide" as used herein refers to a polymeric form of nucleotides of any length, either ribonucleotides or deoxyribonucleotides. This term refers only to the primary structure of the molecule. Thus, this term includes double- and single-stranded DNA and RNA. It also includes known types of modifications, for example, labels which are known in the art, methylation, "caps", substitution of one or more of the naturally occurring nucleotides with an analog, internucleotide modifications such as, for example, those with uncharged linkages (e.g., methyl phosphonates, phosphotriesters, phosphoamidates, carbamates, etc.) and with charged linkages (e.g., phosphorothioates, phosphorodithioates, etc.), those containing pendant moieties, such as, for example proteins (including for e.g., nucleases, toxins, antibodies, signal peptides, poly-L-lysine, etc.), those with intercalators (e.g., acridine, psoralen, etc.), those containing chelators (e.g., metals, radioactive metals, boron, oxidative metals, etc.), those containing alkylators, those with modified linkages (e.g., alpha anomeric nucleic acids, etc.), as well as unmodified forms of the polynucleotide.
As used herein, the "sense strand" of a nucleic acid contains the sequence that has sequence homology to that of mRNA. The "anti-sense strand" contains a sequence which is complementary to that of the "sense strand".
As used herein, a "positive stranded genome" of a virus is one in which the genome, whether RNA or DNA, is single-stranded and which encodes a viral polypeptide(s). Examples of positive stranded RNA viruses include Togaviridae, Coronaviridae, Retroviridae, Picornaviridae, and Caliciviridaeo Included also, are the Flaviviridae, which were formerly classified as Togaviradae. See Fields & Knipe (1986).
The term "primer" as used herein refers to an oligomer which is capable of acting as a point of initiation of synthesis of a polynucleotide strand when placed under appropriate conditions. The primer will be completely or substantially complementary to a region of the polynucleotide strand to be copied. Thus, under conditions conducive to hybridization, the primer will anneal to the complementary region of the analyte strand. Upon addition of suitable reactants, (e.g., a polymerase, nucleotide triphosphates, and the like), the primer is extended by the polymerizing agent to form a copy of the analyte strand. The primer may be single-stranded, or alternatively maybe partially or fully double-stranded.
The terms "analyte polynucleotide" and "analyte strand" refer to a single- or double-stranded nucleic acid molecule which is suspected of containing a target sequence, and which may be present in a biological sample.
As used herein, the term "oligomer" refers to primers and to probes. The term oligomer does not connote the size of the molecule. However, typically oligomers are no greater than 1000 nucleotides, more typically are no greater than 500 nucleotides, even more typically are no greater than 250 nucleotides; they may be no greater than 100 nucleotides, and may be no greater than 75 nucleotides, and also may be no greater than 50 nucleotides in length.
As used herein, the term "probe" refers to a structure comprised of a polynucleotide which forms a hybrid structure with a target sequence, due to complementarity of at least one sequence in the probe with a sequence in the target region. The polynucleotide regions of probes may be composed of DNA, and/or RNA, and/or synthetic nucleotide analogs. Included within probes are "capture probes" and "label probes". Preferably the probe does not contain a sequence complementary to sequence(s) used to prime the polymerase chain reaction (PCR).
As used herein, the term "target region" refers to a region of the nucleic acid which is to be amplified and/or detected. The term "target sequence" refers to a sequence with which a probe or primer will form a stable hybrid under desired conditions.
The term "capture probe" as used herein refers to a polynucleotide comprised of a single-stranded polynucleotide coupled to a binding partner. The single-stranded polynucleotide is comprised of a targeting polynucleotide sequence, which is complementary to a target sequence in a target region to be detected in the analyte polynucleotide. This complementary region is of sufficient length and complementarity to the target sequence to afford a duplex of stability which is sufficient to immobilize the analyte polynucleotide to a solid surface (via the binding partners). The binding partner is specific for a second binding partner; the second binding partner can be bound to the surface of a solid support, or may be linked indirectly via other structures or binding partners to a solid support.
The term "targeting polynucleotide sequence" as used herein, refers to a polynucleotide sequence which is comprised of nucleotides which are complementary to a target nucleotide sequence; the sequence is of sufficient length and complementarity with the target sequence to form a duplex which has sufficient stability for the purpose intended.
The term "binding partner" as used herein refers to a molecule capable of binding a ligand molecule with high specificity, as for example an antigen and an antibody specific therefor. In general, the specific binding partners must bind with sufficient affinity to immobilize the analyte copy/complementary strand duplex (in the case of capture probes) under the isolation conditions. Specific binding partners are known in the art, and include, for example, biotin and avidin or streptavidin, IgG and protein A, the numerous known receptor-ligand couples, and complementary polynucleotide strands. In the case of complementary polynucleotide binding partners, the partners are normally at least about 15 bases in length, and may be at least 40 bases in length; in addition, they have a content of Gs and Cs of at least about 40% and as much as about 60%. The polynucleotides may be composed of DNA, RNA, or synthetic nucleotide analogs.
The term "coupled" as used herein refers to attachment by covalent bonds or by strong non-covalent interactions (e.g., hydrophobic interactions, hydrogen bonds, etc.). Covalent bonds may be, for example, ester, ether, phosphoester, amide, peptide, imide, carbon-sulfur bonds, carbon-phosphorus bonds, and the like.
The term "support" refers to any solid or semi-solid surface to which a desired binding partner may be anchored. Suitable supports include glass, plastic, metal, polymer gels, and the like, and may take the form of beads, wells, dipstics, membranes, and the like.
The term "label" as used herein refers to any atom or moiety which can be used to provide a detectable (preferably quantifiable) signal, and which can be attached to a polynucleotide or polypeptide.
As used herein, the term "label probe" refers to an oligomer which is comprised of targeting polynucleotide sequence, which is complementary to a target sequence to be detected in the analyte polynucleotide. This complementary region is of sufficient length and complementarity to the target sequence to afford a duplex comprised of the "label probe" and the "target sequence" to be detected by the label. The oligomer is coupled to a label either directly, or indirectly via a set of ligand molecules with high specificity for each other. Sets of ligand molecules with high specificity are described supra., and also includes multimers.
The term "multimer", as used herein, refers to linear or branched polymers of the same repeating single-stranded polynucleotide unit or different single-stranded polynucleotide units. At least one of the units has a sequence, length, and composition that permits it to hybridize specifically to a first single-stranded nucleotide sequence of interest, typically an analyte or an oligomer (e.g., a label probe) bound to an analyte. In order to achieve such specificity and stability, this unit will normally be at least about 15 nucleotides in length, typically no more than about 50 nucleotides in length, and preferably about 30 nucleotides in length; moreover, the content of Gs and Cs will normally be at least about 40%, and at most about 60%. In addition to such unit(s), the multimer includes a multiplicity of units that are capable of hybridizing specifically and stably to a second single-stranded nucleotide of interest, typically a labeled polynucleotide or another multimer. These units are generally about the same size and composition as the multimers discussed above. When a multimer is designed to be hybridized to another multimer, the first and second oligonucleotide units are heterogeneous (different), and do not hybridize with each other under the conditions of the selected assay. Thus, multimers may be label probes, or may be ligands which couple the label to the probe.
As used herein, the term "viral RNA", which includes HCV RNA, refers to RNA from the viral genome, fragments thereof, transcripts thereof, and mutant sequences derived therefrom.
As used herein, a "biological sample" refers to a sample of tissue or fluid isolated from an individual, including but not limited to, for example, plasma, serum, spinal fluid, lymph fluid, the external sections of the skin, respiratory, intestinal, and genitourinary tracts, tears, saliva, milk, blood cells, tumors, organs, and also samples of in vitro cell culture constituents (including but not limited to conditioned medium resulting from the growth of cells in cell culture medium, putatively virally infected cells, recombinant cells, and cell components).
DESCRIPTION OF THE INVENTION
The practice of the present invention will employ, unless otherwise indicated, conventional techniques of chemistry, molecular biology, microbiology, recombinant DNA, and immunology, which are within the skill of the art." Such techniques are explained fully in the literature. See e.g., Maniatis, Fitsch & Sambrook, MOLECULAR CLONING; A LABORATORY MANUAL (1982); DNA CLONING, VOLUMES I AND II (D. N Glover ed. 1985); OLIGONUCLEOTIDE SYNTHESIS (M. J. Gait ed, 1984); NUCLEIC ACID HYBRIDIZATION (B. D. Hames & S. J. Higgins eds. 1984); the series, METHODS IN ENZYMOLOGY (Academic Press, Inc.), particularly Vol. 154 and Vol. 155 (Wu and Grossman, and Wu, eds., respectively). All patents, patent applications, and publications mentioned herein, both supra and infra, are hereby incorporated herein by reference.
The useful materials and processes of the present invention are made possible by the identification of HCV as the etiologic agent of BB-NANBV, and by the provision of a family of nucleotide sequences isolated from cDNA libraries which contain HCV cDNA sequences. These cDNA libraries were derived from nucleic acid sequences present in the plasma of an HCV-infected chimpanzee. The construction of one of these libraries, the "c" library (ATCC No. 40394), is described in U.S. Pat. No. 5,350,671.
Utilizing the above described HCV cDNA sequences, as well as that described herein, oligomers can be constructed which are useful as reagents for detecting viral polynucleotides in biological samples. For example, from the sequences it is possible to synthesize DNA oligomers of about 8-10 nucleotides, or larger, which are useful as hybridization probes to detect the presence of HCV RNA in, for example, donated blood, blood fractions, sera of subjects suspected of harboring the virus, or cell culture systems in which the virus is replicating. In addition, the novel oligomers described herein enable further characterization of the HCV genome. Polynucleotide probes and primers derived from these sequences may be used to amplify sequences present in cDNA libraries, and/or to screen cDNA libraries for additional overlapping cDNA Sequences, which, in turn, may be used to obtain more overlapping sequences. As indicated infra. and in U.S. Pat. No. 5,350,671, the genome of HCV appears to be RNA comprised primarily of a large open reading frame (ORF) which encodes a large polyprotein.
In addition to the above, the information provided infra allows the identification of additional HCV strains or isolates. The isolation and characterization of the additional HCV strains or isolates may be accomplished by, for example, isolating the nucleic acids from body components which contain viral particles and/or viral RNA, creating cDNA libraries using the oligomers described infra., for screening the libraries for clones containing HCV cDNA sequences described infra., and comparing the HCV cDNAs from the new isolates with the cDNAs described in U.S. Pat. No. 5,350,671 and infra. Strains or isolates which fit within the parameters of HCV, as described in the Definitions section, supra., are readily identifiable. Other methods for identifying HCV strains will be obvious to those of skill in the art, based upon the information provided herein.
Isolation of the HCV cDNA Sequences
The oligomers of the invention contain regions which form hybrid duplex structures with targeted sequences in HCV polynucleotides. The HCV polynucleotide hybridizing regions of the oligomers may be ascertained from the HCV cDNA sequence(s) provided herein, and described in U.S. Pat. No. 5,350,671. A composite of HCV cDNA from HCV1, a prototypic HCV, is shown in FIG. 1. The composite sequence is based upon sequence information derived from a number of HCV cDNA clones, which were isolated from a number of HCV cDNA libraries, including the "c" library present in lambda gt11 (ATCC No. 40394), and from human serum. The HCV-cDNA clones were isolated by methods described in U.S. Pat. No. 5,350,671. Briefly, the majority of clones which were isolated contained sequences from the HCV cDNA "c" library which was constructed using pooled serum from a chimpanzee with chronic HCV infection and containing a high titer of the virus, i.e., at least 10.sup.6 chimp infectious doses/ml (CID/ml). The pooled serum was used to isolate viral particles; nucleic acids isolated from these particles was used as the template in the construction of cDNA libraries to the viral genome. The initial clone, 5-1-1, was obtained by screening the "c" library with serum from infected individuals. After the isolation of the initial clone, the remainder of the sequence was obtained by screening with synthetic polynucleotide probes, the sequences of which were derived from the 5'-region and the 3'-region of the known HCV cDNA sequence(s).
The description of the methods to retrieve the cDNA sequences is mostly of historical interest. The resultant sequences (and their complements) are provided herein, and the sequences, or any portion thereof, could be prepared using synthetic methods, or by a combination of synthetic methods with retrieval of partial sequences Using methods similar to those described in U.S. Pat. No. 5,350,671.
Oligomer Probes and Primers
Using as a basis the HCV genome (as illustrated in FIG. 1), and/or preferably conserved regions of the HCV genome, oligomers of approximately 8 nucleotides or more can be prepared which hybridize with the positive strand(s) of HCV RNA or its complement, as well as to HCV cDNAs. These oligomers can serve as probes for the detection (including isolation and/or labeling) of polynucleotides which contain HCV nucleotide sequences,. and/or as primers for the transcription and/or replication of targeted HCV sequences. The oligomers contain a targeting polynucleotide sequence, which is comprised of nucleotides which are complementary to a target HCV nucleotide sequence; the sequence is of sufficient length and complementarity with the HCV sequence to form a duplex which has sufficient stability for the purpose intended. For example, if the purpose is the isolation, via immobilization, of an analyte containing a target HCV sequence, the oligomers would contain a polynucleotide region which is of sufficient length and complementarity to the targeted HCV sequence to afford sufficient duplex stability to immobilize the analyte on a solid surface, via its binding to the oligomers, under the isolation conditions. For example, also, if the oligomers are to serve as primers for the transcription and/or-replication of target HCV sequences in an analyte polynucleotide, the oligomers would contain a polynucleotide region of sufficient length and complementarity to the targeted HCV sequence to allow the polymerizing agent to continue replication from the primers which are in stable duplex form with the target sequence, under the polymerizing conditions. For example, also, if the oligomers are to be used as label probes, or are to bind to multimers, the targeting polynucleotide region would be of sufficient length and complementarity to form stable hybrid duplex structures with the label probes and/or multimers to allow detection of the duplex. The oligomers may contain a minimum of about 4 contiguous nucleotides which are complementary to targeted HCV sequence; usually the oligomers will contain a minimum of about 8 continguous nucleotides which are complementary to the targeted HCV sequence, and preferably will contain a minimum of about 14 contiguous nucleotides which are complementary to the targeted HCV sequence.
Suitable HCV nucleotide targeting sequences may be comprised of nucleotides which are complementary nucleotides selected from the following HCV cDNA nucleotides, which are shown in FIG. 1, (nn.sub.x -nn.sub.y denotes from about nucleotide number x to about nucleotide number y)):
nn.sub.-340 -nn.sub.-330 ; nn.sub.-330 -nn.sub.-320 ; nn.sub.-320 -nn.sub.-310 ;
nn.sub.-310 -nn.sub.-300 ; nn.sub.-300 -nn.sub.-290 ; nn.sub.-290 -nn.sub.-280 ;
nn.sub.-280 -nn.sub.-270 ; nn.sub.-270 -nn.sub.-260 ; nn.sub.-260 -nn.sub.-250 ;
nn.sub.-250 -nn.sub.-240 ; nn.sub.-240 -nn.sub.-230 ; nn.sub.-230 -nn.sub.-220 ;
nn.sub.-220 -nn.sub.-210 ; nn.sub.-210 -nn.sub.-200 ; nn.sub.-200 -nn.sub.-190 ;
nn.sub.-190 -nn.sub.-180 ; nn.sub.-180 -nn.sub.-170 ; nn.sub.-170 -nn.sub.-160 ;
nn.sub.-160 -nn.sub.-150 ; nn.sub.-150 -nn.sub.-140 ; nn.sub.-140 -nn.sub.-130 ;
nn.sub.-130 -nn.sub.-120 ; nn.sub.-120 -nn.sub.-110 ; nn.sub.-110 -nn.sub.-100 ;
nn.sub.-100 -nn.sub.-90 ; nn.sub.-90 -nn.sub.-80 ; nn.sub.-80 -nn.sub.-70 ;
nn.sub.-70 -nn.sub.-60 ; nn.sub.-60 -nn.sub.-50 ; nn.sub.-50 -nn.sub.-40 ;
nn.sub.-40 -nn.sub.-30 ; nn.sub.-30 -nn.sub.-20 ; nn.sub.-20 -nn.sub.-10 ;
nn.sub.-10 -nn.sub.1 ; nn.sub.1 -nn.sub.10 ; nn.sub.10 -nn.sub.20 ; nn.sub.20 -nn.sub.30 ;
nn.sub.30 -nn.sub.40 ; nn.sub.40 -nn.sub.50 ; nn.sub.50 -nn.sub.60 ; nn.sub.60 -nn.sub.70 ;
nn.sub.70 -nn.sub.80 ; nn.sub.80 -nn.sub.90 ; nn.sub.90 -nn.sub.100 ; nn.sub.100 -nn.sub.110 ;
nn.sub.110 -nn.sub.120 ; nn.sub.120 -nn.sub.130 ; nn.sub.130 -nn.sub.140 ;
nn.sub.140 -nn.sub.150 ; nn.sub.150 -nn.sub.160 ; nn.sub.160 -nn.sub.170 ;
nn.sub.170 -nn.sub.180 ; nn.sub.180 -nn.sub.190 ; nn.sub.190 -nn.sub.200 ;
nn.sub.200 -nn.sub.210 ; nn.sub.210 -nn.sub.220 ; nn.sub.220 -nn.sub.230 ;
nn.sub.230 -nn.sub.240 ; nn.sub.240 -nn.sub.250 ; nn.sub.250 -nn.sub.260 ;
nn.sub.260 -nn.sub.270 ; nn.sub.270 -nn.sub.280 ; nn.sub.280 -nn.sub.290 ;
nn.sub.290 -nn.sub.300 ; nn.sub.300 -nn.sub.310 ; nn.sub.310 -nn.sub.320 ;
nn.sub.320 -nn.sub.330 ; nn.sub.330 -nn.sub.340 ; nn.sub.340 -nn.sub.350 ;
nn.sub.350 -nn.sub.360 ; nn.sub.360 -nn.sub.370 ; nn.sub.370 -nn.sub.380 ;
nn.sub.380 -nn.sub.390 ; nn.sub.390 -nn.sub.400 ; nn.sub.400 -nn.sub.410 ;
nn.sub.410 -nn.sub.420 ; nn.sub.420 -nn.sub.430 ; nn.sub.430 -nn.sub.440 ;
nn.sub.440 -nn.sub.450 ; nn.sub.450 -nn.sub.460 ; nn.sub.460 -nn.sub.470 ;
nn.sub.470 -nn.sub.480 ; nn.sub.480 -nn.sub.490 ; nn.sub.490 -nn.sub.500 ;
nn.sub.500 -nn.sub.510 ; nn.sub.510 -nn.sub.520 ; nn.sub.520 -nn.sub.530 ;
nn.sub.530 -nn.sub.540 ; nn.sub.540 -nn.sub.550 ; nn.sub.550 -nn.sub.560 ;
nn.sub.560 -nn.sub.570 ; nn.sub.570 -nn.sub.580 ; nn.sub.580 -nn.sub.590 ;
nn.sub.590 -nn.sub.600 ; nn.sub.600 -nn.sub.610 ; nn.sub.610 -nn.sub.620 ;
nn.sub.620 -nn.sub.630 ; nn.sub.630 -nn.sub.640 ; nn.sub.640 -nn.sub.650 ;
nn.sub.650 -nn.sub.660 ; nn.sub.660 -nn.sub.670 ; nn.sub.670 -nn.sub.680 ;
nn.sub.680 -nn.sub.690 ; nn.sub.690 -nn.sub.700 ; nn.sub.700 -nn.sub.710 ;
nn.sub.710 -nn.sub.720 ; nn.sub.720 -nn.sub.730 ; nn.sub.730 -nn.sub.740 ;
nn.sub.740 -nn.sub.750 ; nn.sub.750 -nn.sub.760 ; nn.sub.760 -nn.sub.770 ;
nn.sub.770 -nn.sub.780 ; nn.sub.780 -nn.sub.790 ; nn.sub.790 -nn.sub.800 ;
nn.sub.800 -nn.sub.810 ; nn.sub.810 -nn.sub.820 ; nn.sub.820 -nn.sub.830 ;
nn.sub.830 -nn.sub.840 ; nn.sub.840 -nn.sub.850 ; nn.sub.850 -nn.sub.860 ;
nn.sub.860 -nn.sub.870 ; nn.sub.870 -nn.sub.880 ; nn.sub.880 -nn.sub.890 ;
nn.sub.890 -nn.sub.900 ; nn.sub.900 -nn.sub.910 ; nn.sub.910 -nn.sub.920 ;
nn.sub.920 -nn.sub.930 ; nn.sub.930 -nn.sub.940 ; nn.sub.940 -nn.sub.950 ;
nn.sub.950 -nn.sub.960 ; nn.sub.960 -nn.sub.970 ; nn.sub.970 -nn.sub.980;
nn.sub.980 -nn.sub.990 ; nn.sub.990 -nn.sub.1000 ; nn.sub.1000 -nn.sub.1010 ;
nn.sub.1010 -nn.sub.1020 ; nn.sub.1020 -nn.sub.1030 ; nn.sub.1030 -nn.sub.1040 ;
nn.sub.1040 -nn.sub.1050 ; nn.sub.1050 -nn.sub.1060 ; nn.sub.1060 -nn.sub.1070 ;
nn.sub.1070 -nn.sub.1080 ; nn.sub.1080 -nn.sub.1090 ; nn.sub.1090 -nn.sub.1100 ;
nn.sub.1100 -nn.sub.1110 ; nn.sub.1110 -nn.sub.1120 ; nn.sub.1120 -nn.sub.1130 ;
nn.sub.1130 -nn.sub.1140 ; nn.sub.1140 -nn.sub.1150 ; nn.sub.1150 -nn.sub.1160 ;
nn.sub.1160 -nn.sub.1170 ; nn.sub.1170 -nn.sub.1180 ; nn.sub.1180 -nn.sub.1190 ;
nn.sub.1190 -nn.sub.1200 ; nn.sub.1200 -nn.sub.1210 ; nn.sub.1210 -nn.sub.1220 ;
nn.sub.1220 -nn.sub.1230 ; nn.sub.1230 -nn.sub.1240 ; nn.sub.1240 -nn.sub.1250 ;
nn.sub.1250 -nn.sub.1260 ; nn.sub.1260 -nn.sub.1270 ; nn.sub.1270 -nn.sub.1280 ;
nn.sub.1280 -nn.sub.1290 ; nn.sub.1290 -nn.sub.1300 ; nn.sub.1300 -nn.sub.1310 ;
nn.sub.1310 -nn.sub.1320 ; nn.sub.1320 -nn.sub.1330 ; nn.sub.1330 -nn.sub.1340 ;
nn.sub.1340 -nn.sub.1350 ; nn.sub.1350 -nn.sub.1360 ; nn.sub.1360 -nn.sub.1370 ;
nn.sub.1370 -nn.sub.1380 ; nn.sub.1380 -nn.sub.1390 ; nn.sub.1390 -nn.sub.1400 ;
nn.sub.1400 -nn.sub.1410 ; nn.sub.1410 -nn.sub.1420 ; nn.sub.1420 -nn.sub.1430 ;
nn.sub.1430 -nn.sub.1440 ; nn.sub.1440 -nn.sub.1450 ; nn.sub.1450 -nn.sub.1460 ;
nn.sub.1460 -nn.sub.1470 ; nn.sub.1470 -nn.sub.1480 ; nn.sub.1480 -nn.sub.1490 ;
nn.sub.1490 -nn.sub.1500 ; nn.sub.1500 -nn.sub.1510 ; nn.sub.1510 -nn.sub.1520 ;
nn.sub.1520 -nn.sub.1530 ; nn.sub.1530 -nn.sub.1540 ; nn.sub.1540 -nn.sub.1550 ;
nn.sub.1550 -nn.sub.1560 ; nn.sub.1560 -nn.sub.1570 ; nn.sub.1570 -nn.sub.1580 ;
nn.sub.1640 -nn.sub.1650 ; nn.sub.1650 -nn.sub.1660 ; nn.sub.1660 -nn.sub.1670 ;
nn.sub.1580 -nn.sub.1590 ; nn.sub.1590 -nn.sub.1600 ; nn.sub.1600 -nn.sub.1610 ;
nn.sub.1610 -nn.sub.1620 ; nn.sub.1620 -nn.sub.1630 ; nn.sub.1630 -nn.sub.1640 ;
nn.sub.1670 -nn.sub.1680 ; nn.sub.1680 -nn.sub.1690 ; nn.sub.1690 -nn.sub.1700 ;
nn.sub.1700 -nn.sub.1710 ; nn.sub.1710 -nn.sub.1720 ; nn.sub.1720 -nn.sub.1730 ;
nn.sub.1730 -nn.sub.1740 ; nn.sub.1740 -nn.sub.1750 ; nn.sub.1750 -nn.sub.1760 ;
nn.sub.1760 -nn.sub.1770 ; nn.sub.1770 -nn.sub.1780 ; nn.sub.1780 -nn.sub.1790 ;
nn.sub.1790 -nn.sub.1800 ; nn.sub.1800 -nn.sub.1810 ; nn.sub.1810 -nn.sub.1820 ;
nn.sub.1820 -nn.sub.1830 ; nn.sub.1830 -nn.sub.1840 ; nn.sub.1840 -nn.sub.1850 ;
nn.sub.1850 -nn.sub.1860 ; nn.sub.1860 -nn.sub.1870 ; nn.sub.1870 -nn.sub.1880 ;
nn.sub.1880 -nn.sub.1890 ; nn.sub.1890 -nn.sub.1900 ; nn.sub.1900 -nn.sub.1910 ;
nn.sub.1910 -nn.sub.1920 ; nn.sub.1920 -nn.sub.1930 ; nn.sub.1930 -nn.sub.1940 ;
nn.sub.1940 -nn.sub.1950 ; nn.sub.1950 -nn.sub.1960 ; nn.sub.1960 -nn.sub.1970 ;
nn.sub.2030 -nn.sub.2040 ; nn.sub.2040 -nn.sub.2050 ; nn.sub.2050 -nn.sub.2060 ;
nn.sub.1970 -nn.sub.1980 ; nn.sub.1980 -nn.sub.1990 ; nn.sub.1990 -nn.sub.2000 ;
nn.sub.2000 -nn.sub.2010 ; nn.sub.2010 -nn.sub.2020 ; nn.sub.2020 -nn.sub.2030 ;
nn.sub.2060 -nn.sub.2070 ; nn.sub.2070 -nn.sub.2080 ; nn.sub.2080 -nn.sub.2090 ;
nn.sub.2090 -nn.sub.2100 ; nn.sub.2100 -nn.sub.2110 ; nn.sub.2110 -nn.sub.2120 ;
nn.sub.2120 -nn.sub.2130 ; nn.sub.2130 -nn.sub.2140 ; nn.sub.2140 -nn.sub.2150 ;
nn.sub.2150 -nn.sub.2160 ; nn.sub.2160 -nn.sub.2170 ; nn.sub.2170 -nn.sub.2180 ;
nn.sub.2180 -nn.sub.2190 ; nn.sub.2190 -nn.sub.2200 ; nn.sub.2200 -nn.sub.2210 ;
nn.sub.2210 -nn.sub.2220 ; nn.sub.2220 -nn.sub.2230 ; nn.sub.2230 -nn.sub.2240 ;
nn.sub.2240 -nn.sub.2250 ; nn.sub.2250 -nn.sub.2260 ; nn.sub.2260 -nn.sub.2270 ;
nn.sub.2270 -nn.sub.2280 ; nn.sub.2280 -nn.sub.2290 ; nn.sub.2290 -nn.sub.2300 ;
nn.sub.2300 -nn.sub.2310 ; nn.sub.2310 -nn.sub.2320 ; nn.sub.2320 -nn.sub.2330 ;
nn.sub.2330 -nn.sub.2340 ; nn.sub.2340 -nn.sub.2350 ; nn.sub.2350 -nn.sub.2360 ;
nn.sub.2360 -nn.sub.2370 ; nn.sub.2370 -nn.sub.2380 ; nn.sub.2380 -nn.sub.2390 ;
nn.sub.2390 -nn.sub.2400 ; nn.sub.2400 -nn.sub.2410 ; nn.sub.2410 -nn.sub.2420 ;
nn.sub.2420 -nn.sub.2430 ; nn.sub.2430 -nn.sub.2440 ; nn.sub.2440 -nn.sub.2450 ;
nn.sub.2450 -nn.sub.2460 ; nn.sub.2460 -nn.sub.2470 ; nn.sub.2470 -nn.sub.2480 ;
nn.sub.2480 -nn.sub.2490 ; nn.sub.2490 -nn.sub.2500 ; nn.sub.2500 -nn.sub.2510 ;
nn.sub.2510 -nn.sub.2520 ; nn.sub.2520 -nn.sub.2530 ; nn.sub.2530 -nn.sub.2540 ;
nn.sub.2540 -nn.sub.2550 ; nn.sub.2550 -nn.sub.2560 ; nn.sub.2560 -nn.sub.2570 ;
nn.sub.2570 -nn.sub.2580 ; nn.sub.2580 -nn.sub.2590 ; nn.sub.2590 -nn.sub.2600 ;
nn.sub.2600 -nn.sub.2610 ; nn.sub.2610 -nn.sub.2620 ; nn.sub.2620 -nn.sub.2630 ;
nn.sub.2630 -nn.sub.2640 ; nn.sub.2640 -nn.sub.2650 ; nn.sub.2650 -nn.sub.2660 ;
nn.sub.2660 -nn.sub.2670 ; nn.sub.2670 -nn.sub.2680 ; nn.sub.2680 -nn.sub.2690 ;
nn.sub.2690 -nn.sub.2700 ; nn.sub.2700 -nn.sub.2710 ; nn.sub.2710 -nn.sub.2720 ;
nn.sub.2720 -nn.sub.2730 ; nn.sub.2730 -nn.sub.2740 ; nn.sub.2740 -nn.sub.2750 ;
nn.sub.2750 -nn.sub.2760 ; nn.sub.2760 -nn.sub.2770 ; nn.sub.2770 -nn.sub.2780 ;
nn.sub.2780 -nn.sub.2790 ; nn.sub.2790 -nn.sub.2800 ; nn.sub.2800 -nn.sub.2810 ;
nn.sub.2810 -nn.sub.2820 ; nn.sub.2820 -nn.sub.2830 ; nn.sub.2830 -nn.sub.2840 ;
nn.sub.2840 -nn.sub.2850 ; nn.sub.2850 -nn.sub.2860 ; nn.sub.2860 -nn.sub.2870 ;
nn.sub.2870 -nn.sub.2880 ; nn.sub.2880 -nn.sub.2890 ; nn.sub.2890 -nn.sub.2900 ;
nn.sub.2900 -nn.sub.2910 ; nn.sub.2910 -nn.sub.2920 ; nn.sub.2920 -nn.sub.2930 ;
nn.sub.2930 -nn.sub.2940 ; nn.sub.2940 -nn.sub.2950 ; nn.sub.2950 -nn.sub.2960 ;
nn.sub.2960 -nn.sub.2970 ; nn.sub.2970 -nn.sub.2980 ; nn.sub.2980 -nn.sub.2990 ;
nn.sub.2990 -nn.sub.3000 ; nn.sub.3000 -nn.sub.3010 ; nn.sub.3010 -nn.sub.3020 ;
nn.sub.3020 -nn.sub.3030 ; nn.sub.3030 -nn.sub.3040 ; nn.sub.3040 -nn.sub.3050 ;
nn.sub.3050 -nn.sub.3060 ; nn.sub.3060 -nn.sub.3070 ; nn.sub.3070 -nn.sub.3080 ;
nn.sub.3080 -nn.sub.3090 ; nn.sub.3090 -nn.sub.3100 ; nn.sub.3109 -nn.sub.3110 ;
nn.sub.3110 -nn.sub.3120 ; nn.sub.3120 -nn.sub.3130 ; nn.sub.3130 -nn.sub.3140 ;
nn.sub.3140 -nn.sub.3150 ; nn.sub.3150 -nn.sub.3160 ; nn.sub.3160 -nn.sub.3170 ;
nn.sub.3170 -nn.sub.3180 ; nn.sub.3180 -nn.sub.3190 ; nn.sub.3190 -nn.sub.3200 ;
nn.sub.3200 -nn.sub.3210 ; nn.sub.3210 -nn.sub.3220 ; nn.sub.3220 -nn.sub.3230 ;
nn.sub.3230 -nn.sub.3240 ; nn.sub.3240 -nn.sub.3250 ; nn.sub.3250 -nn.sub.3260 ;
nn.sub.3260 -nn.sub.3270 ; nn.sub.3270 -nn.sub.3280 ; nn.sub.3280 -nn.sub.3290 ;
nn.sub.3290 -nn.sub.3300 ; nn.sub.3300 -nn.sub.3310 ; nn.sub.3310 -nn.sub.3320 ;
nn.sub.3320 -nn.sub.3330 ; nn.sub.3330 -nn.sub.3340 ; nn.sub.3340 -nn.sub.3350 ;
nn.sub.3350 -nn.sub.3360 ; nn.sub.3360 -nn.sub.3370 ; nn.sub.3370 -nn.sub.3380 ;
nn.sub.3380 -nn.sub.3390 ; nn.sub.3390 -nn.sub.3400 ; nn.sub.3400 -nn.sub.3410 ;
nn.sub.3410 -nn.sub.3420 ; nn.sub.3420 -nn.sub.3430 ; nn.sub.3430 -nn.sub.3440 ;
nn.sub.3440 -nn.sub.3450 ; nn.sub.3450 -nn.sub.3460 ; nn.sub.3460 -nn.sub.3470 ;
nn.sub.3470 -nn.sub.3480 ; nn.sub.3480 -nn.sub.3490 ; nn.sub.3490 -nn.sub.3500 ;
nn.sub.3500 -nn.sub.3510 ; nn.sub.3510 -nn.sub.3520 ; nn.sub.3520 -nn.sub.3530 ;
nn.sub.3530 -nn.sub.3540 ; nn.sub.3540 -nn.sub.3550 ; nn.sub.3550 -nn.sub.3560 ;
nn.sub.3560 -nn.sub.3570 ; nn.sub.3570 -nn.sub.3580 ; nn.sub.3580 -nn.sub.3590 ;
nn.sub.3590 -nn.sub.3600 ; nn.sub.3600 -nn.sub.3610 ; nn.sub.3610 -nn.sub.3620 ;
nn.sub.3620 -nn.sub.3630 ; nn.sub.3630 -nn.sub.3640 ; nn.sub.3640 -nn.sub.3650 ;
nn.sub.3650 -nn.sub.3660 ; nn.sub.3660 -nn.sub.3670 ; nn.sub.3670 -nn.sub.3680 ;
nn.sub.3680 -nn.sub.3690 ; nn.sub.3690 -nn.sub.3700 ; nn.sub.3700 -nn.sub.3710 ;
nn.sub.3710 -nn.sub.3720 ; nn.sub.3720 -nn.sub.3730 ; nn.sub.3730 -nn.sub.3740 ;
nn.sub.3740 -nn.sub.3750 ; nn.sub.3750 -nn.sub.3760 ; nn.sub.3760 -nn.sub.3770 ;
nn.sub.3770 -nn.sub.3780 ; nn.sub.3780 -nn.sub.3790 ; nn.sub.3790 -nn.sub.3800 ;
nn.sub.3800 -nn.sub.3810 ; nn.sub.3810 -nn.sub.3820 ; nn.sub.3820 -nn.sub.3830 ;
nn.sub.3830 -nn.sub.3840 ; nn.sub.3840 -nn.sub.3850 ; nn.sub.3850 -nn.sub.3860 ;
nn.sub.3860 -nn.sub.3870 ; nn.sub.3870 -nn.sub.3880 ; nn.sub.3880 -nn.sub.3890 ;
nn.sub.3890 -nn.sub.3900 ; nn.sub.3900 -nn.sub.3910 ; nn.sub.3910 -nn.sub.3920 ;
nn.sub.3920 -nn.sub.3930 ; nn.sub.3930 -nn.sub.3940 ; nn.sub.3940 -nn.sub.3950 ;
nn.sub.3950 -nn.sub.3960 ; nn.sub.3960 -nn.sub.3970 ; nn.sub.3970 -nn.sub.3980 ;
nn.sub.3980 -nn.sub.3990 ; nn.sub.3990 -nn.sub.4000 ; nn.sub.4000 -nn.sub.4010 ;
nn.sub.4010 -nn.sub.4020 ; nn.sub.4020 -nn.sub.4030 ; nn.sub.4030 -nn.sub.4040 ;
nn.sub.4040 -nn.sub.4050 ; nn.sub.4050 -nn.sub.4060 ; nn.sub.4060 -nn.sub.4070 ;
nn.sub.4070 -nn.sub.4080 ; nn.sub.4080 -nn.sub.4090 ; nn.sub.4090 -nn.sub.4100 ;
nn.sub.4100 -nn.sub.4110 ; nn.sub.4110 -nn.sub.4120 ; nn.sub.4120 -nn.sub.4130 ;
nn.sub.4130 -nn.sub.4140 ; nn.sub.4140 -nn.sub.4150 ; nn.sub.4150 -nn.sub.4160 ;
nn.sub.4160 -nn.sub.4170 ; nn.sub.4170 -nn.sub.4180 ; nn.sub.4180 -nn.sub.4190 ;
nn.sub.4190 -nn.sub.4200 ; nn.sub.4200 -nn.sub.4210 ; nn.sub.4210 -nn.sub.4220 ;
nn.sub.4220 -nn.sub.4230 ; nn.sub.4230 -nn.sub.4240 ; nn.sub.4240 -nn.sub.4250 ;
nn.sub.4250 -nn.sub.4260 ; nn.sub.4260 -nn.sub.4270 ; nn.sub.4270 -nn.sub.4280 ;
nn.sub.4280 -nn.sub.4290 ; nn.sub.4290 -nn.sub.4300 ; nn.sub.4300 -nn.sub.4310 ;
nn.sub.4310 -nn.sub.4320 ; nn.sub.4320 -nn.sub.4330 ; nn.sub.4330 -nn.sub.4340 ;
nn.sub.4340 -nn.sub.4350 ; nn.sub.4350 -nn.sub.4360 ; nn.sub.4360 -nn.sub.4370 ;
nn.sub.4370 -nn.sub.4380 ; nn.sub.4380 -nn.sub.4390 ; nn.sub.4390 -nn.sub.4400 ;
nn.sub.4400 -nn.sub.4410 ; nn.sub.4410 -nn.sub.4420 ; nn.sub.4420 -nn.sub.4430 ;
nn.sub.4430 -nn.sub.4440 ; nn.sub.4440 -nn.sub.4450 ; nn.sub.4450 -nn.sub.4460 ;
nn.sub.4460 -nn.sub.4470 ; nn.sub.4470 -nn.sub.4480 ; nn.sub.4480 -nn.sub.4490 ;
nn.sub.4490 -nn.sub.4500 ; nn.sub.4500 -nn.sub.4510 ; nn.sub.4510 -nn.sub.4520 ;
nn.sub.4520 -nn.sub.4530 ; nn.sub.4530 -nn.sub.4540 ; nn.sub.4540 -nn.sub.4550 ;
nn.sub.4550 -nn.sub.4560 ; nn.sub.4560 -nn.sub.4570 ; nn.sub.4570 -nn.sub.4580 ;
nn.sub.4580 -nn.sub.4590 ; nn.sub.4590 -nn.sub.4600 ; nn.sub.4600 -nn.sub.4610 ;
nn.sub.4610 -nn.sub.4620 ; nn.sub.4620 -nn.sub.4630 ; nn.sub.4630 -nn.sub.4640 ;
nn.sub.4640 -nn.sub.4650 ; nn.sub.4650 -nn.sub.4660 ; nn.sub.4660 -nn.sub.4670 ;
nn.sub.4670 -nn.sub.4680 ; nn.sub.4680 -nn.sub.4690 ; nn.sub.4690 -nn.sub.4700 ;
nn.sub.4700 -nn.sub.4710 ; nn.sub.4710 -nn.sub.4720 ; nn.sub.4720 -nn.sub.4730 ;
nn.sub.4730 -nn.sub.4740 ; nn.sub.4740 -nn.sub.4750 ; nn.sub.4750 -nn.sub.4760 ;
nn.sub.4760 -nn.sub.4770 ; nn.sub.4770 -nn.sub.4780 ; nn.sub.4780 -nn.sub.4790 ;
nn.sub.4790 -nn.sub.4800 ; nn.sub.4800 -nn.sub.4810 ; nn.sub.4810 -nn.sub.4820 ;
nn.sub.4820 -nn.sub.4830 ; nn.sub.4830 -nn.sub.4840 ; nn.sub.4840 -nn.sub.4850 ;
nn.sub.4850 -nn.sub.4860 ; nn.sub.4860 -nn.sub.4870 ; nn.sub.4870 -nn.sub.4880 ;
nn.sub.4880 -nn.sub.4890 ; nn.sub.4890 -nn.sub.4900 ; nn.sub.4900 -nn.sub.4910 ;
nn.sub.4910 -nn.sub.4920 ; nn.sub.4920 -nn.sub.4930 ; nn.sub.4930 -nn.sub.4940 ;
nn.sub.4940 -nn.sub.4950 ; nn.sub.4950 -nn.sub.4960 ; nn.sub.4960 -nn.sub.4970 ;
nn.sub.4970 -nn.sub.4980 ; nn.sub.4980 -nn.sub.4990 ; nn.sub.4990 -nn.sub.5000 ;
nn.sub.5000 -nn.sub.5010 ; nn.sub.5010 -nn.sub.5020 ; nn.sub.5020 -nn.sub.5030 ;
nn.sub.5030 -nn.sub.5040 ; nn.sub.5040 -nn.sub.5050 ; nn.sub.5050 -nn.sub.5060 ;
nn.sub.5060 -nn.sub.5070 ; nn.sub.5070 -nn.sub.5080 ; nn.sub.5080 -nn.sub.5090 ;
nn.sub.5090 -nn.sub.5100 ; nn.sub.5100 -nn.sub.5110 ; nn.sub.5110 -nn.sub.5120 ;
nn.sub.5120 -nn.sub.5130 ; nn.sub.5130 -nn.sub.5140 ; nn.sub.5140 -nn.sub.5150 ;
nn.sub.5150 -nn.sub.5160 ; nn.sub.5160 -nn.sub.5170 ; nn.sub.5170 -nn.sub.5180 ;
nn.sub.5180 -nn.sub.5190 ; nn.sub.5190 -nn.sub.5200 ; nn.sub.5200 -nn.sub.5210 ;
nn.sub.5210 -nn.sub.5220 ; nn.sub.5220 -nn.sub.5230 ; nn.sub.5230 -nn.sub.5240 ;
nn.sub.5240 -nn.sub.5250 ; nn.sub.5250 -nn.sub.5260 ; nn.sub.5260 -nn.sub.5270 ;
nn.sub.5270 -nn.sub.5280 ; nn.sub.5280 -nn.sub.5290 ; nn.sub.5290 -nn.sub.5300 ;
nn.sub.5300 -nn.sub.5310 ; nn.sub.5310 -nn.sub.5320 ; nn.sub.5320 -nn.sub.5330 ;
nn.sub.5330 -nn.sub.5340 ; nn.sub.5340 -nn.sub.5350 ; nn.sub.5350 -nn.sub.5360 ;
nn.sub.5360 -nn.sub.5370 ; nn.sub.5370 -nn.sub.5380 ; nn.sub.5380 -nn.sub.5390 ;
nn.sub.5390 -nn.sub.5400 ; nn.sub.5400 -nn.sub.5410 ; nn.sub.5410 -nn.sub.5420 ;
nn.sub.5420 -nn.sub.5430 ; nn.sub.5430 -nn.sub.5440 ; nn.sub.5440 -nn.sub.5450 ;
nn.sub.5450 -nn.sub.5460 ; nn.sub.5460 -nn.sub.5470 ; nn.sub.5470 -nn.sub.5480 ;
nn.sub.5480 -nn.sub.5490 ; nn.sub.5490 -nn.sub.5500 ; nn.sub.5500 -nn.sub.5510 ;
nn.sub.5510 -nn.sub.5520 ; nn.sub.5520 -nn.sub.5530 ; nn.sub.5530 -nn.sub.5540 ;
nn.sub.5540 -nn.sub.5550 ; nn.sub.5550 -nn.sub.5560 ; nn.sub.5560 -nn.sub.5570 ;
nn.sub.5570 -nn.sub.5580 ; nn.sub.5580 -nn.sub.5590 ; nn.sub.5590 -nn.sub.5600 ;
nn.sub.5600 -nn.sub.5610 ; nn.sub.5610 -nn.sub.5620 ; nn.sub.5620 -nn.sub.5630 ;
nn.sub.5630 -nn.sub.5640 ; nn.sub.5640 -nn.sub.5650 ; nn.sub.5650 -nn.sub.5660 ;
nn.sub.5660 -nn.sub.5670 ; nn.sub.5670 -nn.sub.5680 ; nn.sub.5680 -nn.sub.5690 ;
nn.sub.5690 -nn.sub.5700 ; nn.sub.5700 -nn.sub.5710 ; nn.sub.5710 -nn.sub.5720 ;
nn.sub.5720 -nn.sub.5730 ; nn.sub.5730 -nn.sub.5730 ; nn.sub.5740 -nn.sub.5750 ;
nn.sub.5750 -nn.sub.5760 ; nn.sub.5760 -nn.sub.5770 ; nn.sub.5770 -nn.sub.5780 ;
nn.sub.5780 -nn.sub.5790 ; nn.sub.5790 -nn.sub.5800 ; nn.sub.5800 -nn.sub.5810 ;
nn.sub.5810 -nn.sub.5820 ; nn.sub.5820 -nn.sub.5830 ; nn.sub.5830 -nn.sub.5840 ;
nn.sub.5840 -nn.sub.5850 ; nn.sub.5850 -nn.sub.5860 ; nn.sub.5860 -nn.sub.5870 ;
nn.sub.5870 -nn.sub.5880 ; nn.sub.5880 -nn.sub.5890 ; nn.sub.5890 -nn.sub.5900 ;
nn.sub.5900 -nn.sub.5910 ; nn.sub.5910 -nn.sub.5920 ; nn.sub.5920 -nn.sub.5930 ;
nn.sub.5930 -nn.sub.5940 ; nn.sub.5940 -nn.sub.5950 ; nn.sub.5950 -nn.sub.5960 ;
nn.sub.5960 -nn.sub.5970 ; nn.sub.5970 -nn.sub.5980 ; nn.sub.5980 -nn.sub.5990 ;
nn.sub.5990 -nn.sub.6000 ; nn.sub.6000 -nn.sub.6010 ; nn.sub.6010 -nn.sub.6020 ;
nn.sub.6020 -nn.sub.6030 ; nn.sub.6030 -nn.sub.6040 ; nn.sub.6040 -nn.sub.6050 ;
nn.sub.6050 -nn.sub.6060 ; nn.sub.6060 -nn.sub.6070 ; nn.sub.6070 -nn.sub.6080 ;
nn.sub.6080 -nn.sub.6090 ; nn.sub.6090 -nn.sub.6100 ; nn.sub.6100 -nn.sub.6110 ;
nn.sub.6110 -nn.sub.6120 ; nn.sub.6120 -nn.sub.6130 ; nn.sub.6130 -nn.sub.6140 ;
nn.sub.6140 -nn.sub.6150 ; nn.sub.6150 -nn.sub.6160 ; nn.sub.6160 -nn.sub.6170 ;
nn.sub.6170 -nn.sub.6180 ; nn.sub.6180 -nn.sub.6190 ; nn.sub.6190 -nn.sub.6200 ;
nn.sub.6200 -nn.sub.6210 ; nn.sub.6210 -nn.sub.6220 ; nn.sub.6220 -nn.sub.6230 ;
nn.sub.6230 -nn.sub.6240 ; nn.sub.6240 -nn.sub.6250 ; nn.sub.6250 -nn.sub.6260 ;
nn.sub.6260 -nn.sub.6270 ; nn.sub.6270 -nn.sub.6280 ; nn.sub.6280 -nn.sub.6290 ;
nn.sub.6290 -nn.sub.6300 ; nn.sub.6300 -nn.sub.6310 ; nn.sub.6310 -nn.sub.6320 ;
nn.sub.6320 -nn.sub.6330 ; nn.sub.6330 -nn.sub.6340 ; nn.sub.6340 -nn.sub.6350 ;
nn.sub.6350 -nn.sub.6360 ; nn.sub.6360 -nn.sub.6370 ; nn.sub.6370 -nn.sub.6380 ;
nn.sub.6380 -nn.sub.6390 ; nn.sub.6390 -nn.sub.6400 ; nn.sub.6400 -nn.sub.6410 ;
nn.sub.6410 -nn.sub.6420 ; nn.sub.6420 -nn.sub.6430 ; nn.sub.6430 -nn.sub.6440 ;
nn.sub.6440 -nn.sub.6450 ; nn.sub.6450 -nn.sub.6460 ; nn.sub.6460 -nn.sub.6470 ;
nn.sub.6470 -nn.sub.6480 ; nn.sub.6480 -nn.sub.6490 ; nn.sub.6490 -nn.sub.6500 ;
nn.sub.6500 -nn.sub.6510 ; nn.sub.6510 -nn.sub.6520 ; nn.sub.6520 -nn.sub.6530 ;
nn.sub.6530 -nn.sub.6540 ; nn.sub.6540 -nn.sub.6550 ; nn.sub.6550 -nn.sub.6560 ;
nn.sub.6560 -nn.sub.6570 ; nn.sub.6570 -nn.sub.6580 ; nn.sub.6580 -nn.sub.6590 ;
nn.sub.6590 -nn.sub.6600 ; nn.sub.6600 -nn.sub.6610 ; nn.sub.6610 -nn.sub.6620 ;
nn.sub.6620 -nn.sub.6630 ; nn.sub.6630 -nn.sub.6640 ; nn.sub.6640 -nn.sub.6650 ;
nn.sub.6650 -nn.sub.6660 ; nn.sub.6660 -nn.sub.6670 ; nn.sub.6670 -nn.sub.6680 ;
nn.sub.6680 -nn.sub.6690 ; nn.sub.6690 -nn.sub.6700 ; nn.sub.6700 -nn.sub.6710 ;
nn.sub.6710 -nn.sub.6720 ; nn.sub.6720 -nn.sub.6730 ; nn.sub.6730 -nn.sub.6740 ;
nn.sub.6740 -nn.sub.6750 ; nn.sub.6750 -nn.sub.6760 ; nn.sub.6760 -nn.sub.6770 ;
nn.sub.6770 -nn.sub.6780 ; nn.sub.6780 -nn.sub.6790 ; nn.sub.6790 -nn.sub.6800 ;
nn.sub.6800 -nn.sub.6810 ; nn.sub.6810 -nn.sub.6820 ; nn.sub.6820 -nn.sub.6830 ;
nn.sub.6830 -nn.sub.6840 ; nn.sub.6840 -nn.sub.6850 ; nn.sub.6850 -nn.sub.6860 ;
nn.sub.6860 -nn.sub.6870 ; nn.sub.6870 -nn.sub.6880 ; nn.sub.6880 -nn.sub.6890 ;
nn.sub.6890 -nn.sub.6900 ; nn.sub.6900 -nn.sub.6910 ; nn.sub.6910 -nn.sub.6920 ;
nn.sub.6920 -nn.sub.6930 ; nn.sub.6930 -nn.sub.6940 ; nn.sub.6940 -nn.sub.6950 ;
nn.sub.6950 -nn.sub.6960 ; nn.sub.6960 -nn.sub.6970 ; nn.sub.6970 -nn.sub.6980 ;
nn.sub.6980 -nn.sub.6990 ; nn.sub.6990 -nn.sub.7000 ; nn.sub.7000 -nn.sub.7010 ;
nn.sub.7010 -nn.sub.7020 ; nn.sub.7020 -nn.sub.7030 ; nn.sub.7030 -nn.sub.7040 ;
nn.sub.7040 -nn.sub.7050 ; nn.sub.7050 -nn.sub.7060 ; nn.sub.7060 -nn.sub.7070 ;
nn.sub.7070 -nn.sub.7080 ; nn.sub.7080 -nn.sub.7090 ; nn.sub.7090 -nn.sub.7100 ;
nn.sub.7100 -nn.sub.7110 ; nn.sub.7110 -nn.sub.7120 ; nn.sub.7120 -nn.sub.7130 ;
nn.sub.7130 -nn.sub.7140 ; nn.sub.7140 -nn.sub.7150 ; nn.sub.7150 -nn.sub.7160 ;
nn.sub.7160 -nn.sub.7170 ; nn.sub.7170 -nn.sub.7180 ; nn.sub.7180 -nn.sub.7190 ;
nn.sub.7190 -nn.sub.7200 ; nn.sub.7200 -nn.sub.7210 ; nn.sub.7210 -nn.sub.7220 ;
nn.sub.7220 -nn.sub.7230 ; nn.sub.7230 -nn.sub.7240 ; nn.sub.7240 -nn.sub.7250 ;
nn.sub.7250 -nn.sub.7260 ; nn.sub.7260 -nn.sub.7270 ; nn.sub.7270 -nn.sub.7280 ;
nn.sub.7280 -nn.sub.7290 ; nn.sub.7290 -nn.sub.7300 ; nn.sub.7300 -nn.sub.7310 ;
nn.sub.7310 -nn.sub.7320 ; nn.sub.7320 -nn.sub.7330 ; nn.sub.7330 -nn.sub.7340 ;
nn.sub.7340 -nn.sub.7350 ; nn.sub.7350 -nn.sub.7360 ; nn.sub.7360 -nn.sub.7370 ;
nn.sub.7370 -nn.sub.7380 ; nn.sub.7380 -nn.sub.7390 ; nn.sub.7390 -nn.sub.7400 ;
nn.sub.7400 -nn.sub.7410 ; nn.sub.7410 -nn.sub.7420 ; nn.sub.7420 -nn.sub.7430 ;
nn.sub.7430 -nn.sub.7440 ; nn.sub.7440 -nn.sub.7450 ; nn.sub.7450 -nn.sub.7460 ;
nn.sub.7460 -nn.sub.7470 ; nn.sub.7470 -nn.sub.7480 ; nn.sub.7480 -nn.sub.7490 ;
nn.sub.7490 -nn.sub.7500 ; nn.sub.7500 -nn.sub.7510 ; nn.sub.7510 -nn.sub.7520 ;
nn.sub.7520 -nn.sub.7530 ; nn.sub.7530 -nn.sub.7540 ; nn.sub.7540 -nn.sub.7550 ;
nn.sub.7550 -nn.sub.7560 ; nn.sub.7560 -nn.sub.7570 ; nn.sub.7570 -nn.sub.7580 ;
nn.sub.7580 -nn.sub.7590 ; nn.sub.7590 -nn.sub.7600 ; nn.sub.7600 -nn.sub.7610 ;
nn.sub.7610 -nn.sub.7620 ; nn.sub.7620 -nn.sub.7630 ; nn.sub.7630 -nn.sub.7640 ;
nn.sub.7640 -nn.sub.7650 ; nn.sub.7650 -nn.sub.7660 ; nn.sub.7660 -nn.sub.7670 ;
nn.sub.7670 -nn.sub.7680 ; nn.sub.7680 -nn.sub.7690 ; nn.sub.7690 -nn.sub.7700 ;
nn.sub.7700 -nn.sub.7710 ; nn.sub.7710 -nn.sub.7720 ; nn.sub.7720 -nn.sub.7730 ;
nn.sub.7730 -nn.sub.7740 ; nn.sub.7740 -nn.sub.7750 ; nn.sub.7750 -nn.sub.7760 ;
nn.sub.7760 -nn.sub.7770 ; nn.sub.7770 -nn.sub.7780 ; nn.sub.7780 -nn.sub.7790 ;
nn.sub.7790 -nn.sub.7800 ; nn.sub.7800 -nn.sub.7810 ; nn.sub.7810 -nn.sub.7820 ;
nn.sub.7820 -nn.sub.7830 ; nn.sub.7830 -nn.sub.7840 ; nn.sub.7840 -nn.sub.7850 ;
nn.sub.7850 -nn.sub.7860 ; nn.sub.7860 -nn.sub.7870 ; nn.sub.7870 -nn.sub.7880 ;
nn.sub.7880 -nn.sub.7890 ; nn.sub.7890 -nn.sub.7900 ; nn.sub.7900 -nn.sub.7910 ;
nn.sub.7910 -nn.sub.7920 ; nn.sub.7920 -nn.sub.7930 ; nn.sub.7930 -nn.sub.7940 ;
nn.sub.7940 -nn.sub.7950 ; nn.sub.7950 -nn.sub.7960 ; nn.sub.7960 -nn.sub.7970 ;
nn.sub.7970 -nn.sub.7980 ; nn.sub.7980 -nn.sub.7990 ; nn.sub.7990 -nn.sub.8000 ;
nn.sub.8000 -nn.sub.8010 ; nn.sub.8010 -nn.sub.8020 ; nn.sub.8020 -nn.sub.8030 ;
nn.sub.8030 -nn.sub.8040 ; nn.sub.8040 -nn.sub.8050 ; nn.sub.8050 -nn.sub.8060 ;
nn.sub.8060 -nn.sub.8070 ; nn.sub.8070 -nn.sub.8080 ; nn.sub.8080 -nn.sub.8090 ;
nn.sub.8090 -nn.sub.8100 ; nn.sub.8100 -nn.sub.8110 ; nn.sub.8110 -nn.sub.8120 ;
nn.sub.8120 -nn.sub.8130 ; nn.sub.8130 -nn.sub.8140 ; nn.sub.8140 -nn.sub.8150 ;
nn.sub.8150 -nn.sub.8160 ; nn.sub.8160 -nn.sub.8170 ; nn.sub.8170 -nn.sub.8180 ;
nn.sub.8180 -nn.sub.8190 ; nn.sub.8190 -nn.sub.8200 ; nn.sub.8200 -nn.sub.8210 ;
nn.sub.8210 -nn.sub.8220 ; nn.sub.8220 -nn.sub.8230 ; nn.sub.8230 -nn.sub.8240 ;
nn.sub.8240 -nn.sub.8250 ; nn.sub.8250 -nn.sub.8260 ; nn.sub.8260 -nn.sub.8270 ;
nn.sub.8270 -nn.sub.8280 ; nn.sub.8280 -nn.sub.8290 ; nn.sub.8290 -nn.sub.8300 ;
nn.sub.8300 -nn.sub.8310 ; nn.sub.8310 -nn.sub.8320 ; nn.sub.8320 -nn.sub.8330 ;
nn.sub.8330 -nn.sub.8340 ; nn.sub.8340 -nn.sub.8350 ; nn.sub.8350 -nn.sub.8360 ;
nn.sub.8360 -nn.sub.8370 ; nn.sub.8370 -nn.sub.8380 ; nn.sub.8380 -nn.sub.8390 ;
nn.sub.8450 -nn.sub.8460 ; nn.sub.8460 -nn.sub.8470 ; nn.sub.8470 -nn.sub.8480 ;
nn.sub.8390 -nn.sub.8400 ; nn.sub.8400 -nn.sub.8410 ; nn.sub.8410 -nn.sub.8420 ;
nn.sub.8420 -nn.sub.8430 ; nn.sub.8430 -nn.sub.8440 ; nn.sub.8440 -nn.sub.8450 ;
nn.sub.8480 -nn.sub.8490 ; nn.sub.8490 -nn.sub.8500 ; nn.sub.8500 -nn.sub.8510 ;
nn.sub.8510 -nn.sub.8520 ; nn.sub.8520 -nn.sub.8530 ; nn.sub.8530 -nn.sub.8540 ;
nn.sub.8540 -nn.sub.8550 ; nn.sub.8550 -nn.sub.8560 ; nn.sub.8560 -nn.sub.8570 ;
nn.sub.8570 -nn.sub.8580 ; nn.sub.8580 -nn.sub.8590 ; nn.sub.8590 -nn.sub.8600 ;
nn.sub.8600 -nn.sub.8610 ; nn.sub.8610 -nn.sub.8620 ; nn.sub.8620 -nn.sub.8630 ;
nn.sub.8630 -nn.sub.8640 ; nn.sub.8640 -nn.sub.8650 ; nn.sub.8650 -nn.sub.8660 ;
nn.sub.8660 -nn.sub.8670 ; nn.sub.8670 -nn.sub.8680 ; nn.sub.8680 -nn.sub.8690 ;
nn.sub.8690 -nn.sub.8700 ; nn.sub.8700 -nn.sub.8710 ; nn.sub.8710 -nn.sub.8720 ;
nn.sub.8720 -nn.sub.8730 ; nn.sub.8730 -nn.sub.8740 ; nn.sub.8740 -nn.sub.8750 ;
nn.sub.8750 -nn.sub.8760 ; nn.sub.8760 -nn.sub.8770 ; nn.sub.8770 -nn.sub.8780 ;
nn.sub.8780 -nn.sub.8790 ; nn.sub.8790 -nn.sub.8800 ; nn.sub.8800 -nn.sub.8810 ;
nn.sub.8810 -nn.sub.8820 ; nn.sub.8820 -nn.sub.8830 ; nn.sub.8830 -nn.sub.8840 ;
nn.sub.8840 -nn.sub.8850 ; nn.sub.8850 -nn.sub.8860 ; nn.sub.8860 -nn.sub.8870 ;
nn.sub.8870 -nn.sub.8880 ; nn.sub.8880 -nn.sub.8890 ; nn.sub.8890 -nn.sub.8900 ;
nn.sub.8900 -nn.sub.8910 ; nn.sub.8910 -nn.sub.8920 ; nn.sub.8920 -nn.sub.8930 ;
nn.sub.8930 -nn.sub.8940 ; nn.sub.8940 -nn.sub.8950 ; nn.sub.8950 -nn.sub.8960 ;
nn.sub.8960 -nn.sub.8970 ; nn.sub.8970 -nn.sub.8980 ; nn.sub.8980 -nn.sub.8990 ;
nn.sub.8990 -nn.sub.9000 ; nn.sub.9000 -nn.sub.9010 ; nn.sub.9010 -nn.sub.9020 ;
nn.sub.9050 -nn.sub.9060 ; nn.sub.8960 -nn.sub.8970 ; nn.sub.8970 -nn.sub.8980 ;
nn.sub.9020 -nn.sub.9030 ; nn.sub.9030 -nn.sub.9040 ; nn.sub.9040 -nn.sub.9050 ;
The oligomer, however, need not consist only of the sequence which is complementary to the targeted HCV sequence. It may contain in addition, nucleotide sequences or other moieties which are suitable for the purposes for which the oligomers are used. For example, if the oligomers are used as primers for the amplification of HCV sequences via PCR, they may contain sequences which, when in duplex, form restriction enzyme sites which facilitate the cloning of the amplified sequences. For example, also, if the oligomers are to be used as "capture probes" in hybridization assays (described infra), they would contain in addition a binding partner which is coupled to the oligomer containing the nucleotide sequence which is complementary to the targeted HCV sequence. Other types of moieities or sequences which are useful of which the oligomers may be comprised or coupled to, are those which are known in the art to be suitable for a variety of purposes, including the labeling of nucleotide probes.
The preparation of the oligomers is by means known in the art, including, for example, by methods which include excision, transcription, or chemical synthesis. The target sequences and/or regions of the genome which are selected to which the targeting polynucleotides of the oligomers are complementary depend upon the purpose. For example, if the goal is to screen for the presence of HCV in biological samples (e.g. blood), the preferred oligomers would be used as probes and/or primers, and would hybridize to conserved regions of the HCV genome. Some of the conserved regions of the HCV genome to which the oligomers may bind are described herein, for example, the regions which include nucleotide numbers from about the 5-terminus to about 200, or from about 4000 to about 5000, or from about 8000 to about 9040 as shown in FIG. 1, or preferably nucleotides about -318 to about 174, about 4056 to about 4448, and about 4378 to about 4902. Particularly preferred primers and probes are derived from about nucleotides -313 to about -173, and from about nucleotide 1 to about nucleotide 540, as shown in FIG. 1. Other regions of the genome which are conserved are readily ascertainable by comparison of the nucleotide sequences of various isolates of HCV, including the prototype HCV, HCV1. Methods for conducting comparisons between genotypes to determine conserved and nonconserved regions are known in the art, and examples of these methods are disclosed in U.S. Pat. No. 5,350,671, which is incorporated herein by reference.
In the basic nucleic acid hybridization assay, single-stranded analyte nucleic acid (either DNA or RNA) is hybridized to a nucleic acid probe, and resulting duplexes are detected. The probes for HCV polynucleotides (natural or derived) are a length which allows the detection of unique viral sequences by hybridization. While 6-8 nucleotides may be a workable length, sequences of 10-12 nucleotides are preferred, and about 20 nucleotides or more appears optimal. Preferably, these sequences will derive from regions which lack heterogeneity. These probes can be prepared using routine methods, including automated oligonucleotide synthetic methods. Among useful probes, for example, are those derived from the newly isolated clones disclosed herein, as well as the various oligomers useful in probing cDNA libraries, set forth below. A complement to any unique portion of the HCV genome will be satisfactory. For use as probes, complete complementarity is desirable, though it may be unnecessary as the length of the fragment is increased.
For use of such probes as agents to detect the presence of HCV polynucleotides (for example in screening for contaminated blood), the biological sample to be analyzed, such as blood or serum, may be treated, if desired, to extract the nucleic acids contained therein. The resulting nucleic acid from the sample may be subjected to gel electrophoresis or other size separation techniques; alternatively, the nucleic acid sample may be dot blotted without size separation. In order to form hybrid duplexes with the targeting sequence of the probe, the targeted region of the analyte nucleic acid must be in single stranded form. Where the sequence is naturally present in single stranded form, denaturation will not be required. However, where the sequence is present in double stranded form, the sequence will be denatured. Denaturation can be carried out by various techniques known in the art. Subsequent to denaturation, the analyte nucleic acid and probe are incubated under conditions which promote stable hybrid formation of the target sequence in the probe with the putative targeted sequence in the analyte, and the resulting duplexes containing the probe(s) are detected.
Detection of the resulting duplex, if any, is Usually accomplished by the use of labeled probes; alternatively, the probe may be unlabeled, but may be detectable by specific binding with a ligand which is labeled, either directly or indirectly. Suitable labels, and methods for labeling probes and ligands are known in the art, and include, for example, radioactive labels which may be incorporated by known methods (e.g., nick translation or kinasing), biotin, fluorescent groups, chemiluminescent groups (e.g., dioxetanes, particularly triggered dioxetanes), enzymes, antibodies, and the like.
The region of the probes which are used to bind to the analyte can be made completely complementary to the HCV genome. Therefore, usually high stringency conditions are desirable in order to prevent false positives. However, conditions of high stringency should only be used if the probes are complementary to regions of the viral genome which lack heterogeneity. The stringency of hybridization is determined by a number of factors during hybridization and during the washing procedure, including temperature, ionic strength, length of time, and concentration of formamide. These factors are outlined in, for example, Maniatis, T. (1982).
Variations of this basic scheme which are known in the art, including those which facilitate separation of the duplexes to be detected from extraneous materials and/or which amplify the signal from the labeled moiety, may also be used. A number of these variations are reviewed in, for example: Matthews and Kricka (1988), Analytical Biochemistry 169:1; Landegren et al. (1988), Science 242:229; and Mittlin (1989), Clinical chem. 35:1819. These and the following publications describing assay formats are hereby incorporated by reference herein. Probes suitable for detecting HCV in these assays are comprised of sequences which hybridize with target HCV polynucleotide sequences to form duplexes with the analyte strand, wherein the duplexes are of sufficient stability for detection in the specified assay system.
A suitable variation is, for example, one which is described in U.S. Pat. No. 4,868,105, issued Sep. 9, 1989, and in EPO Publication No. 225,807 (published Jun. 16, 1987). These publications describe a solution phase nucleic acid hybridization assay in which the analyte nucleic acid is hybridized to a labeling probe set and to a capturing probe set. The probe-analyte complex is coupled by hybridization with a solid-supported capture probe that is complementary to the capture probe set. This permits the analyte nucleic acid to be removed from solution as a solid phase complex. Having the analyte in the form of a solid phase complex facilitates subsequent separation steps in the assay. The labeling probe set is complementary to a labeled probe that is bound through hybridization to the solid phase/analyte complex.
Generally, it is expected that the HCV genome sequences will be present in serum of infected individuals at relatively low levels, i.e., at approximately 10.sup.2 -10hu 3 chimp infectious doses (CID) per ml. This level may require that amplification techniques be used in hybridization assays. Such techniques are known in the art. For example, the Enzo Biochemical Corporation "Bio-Bridge" system uses terminal deoxynucleotide transferase to add unmodified 3'-poly-dT-tails to a DNA probe. The poly dT-tailed probe is hybridized to the target nucleotide sequence, and then to a biotin-modified poly-A. PCT Publication 84/03520 and EP Publication No. 124221 describe a DNA hybridization assay in which: (1) analyte is annealed to a single-stranded DNA probe that is complementary to an enzyme-labeled oligonucleotide; and (2) the resulting tailed duplex is hybridized to an enzyme-labeled oligonucleotide. EPA 204510 describes a DNA hybridization assay in which analyte DNA is contacted with a probe that has a tail, such as a poly-dT tail, an amplifier strand that has a sequence that hybridizes to the tail of the probe, such as a poly-A sequence, and which is capable of binding a plurality of labeled strands. A type of hybridization assay which is described in EPO Publication No. 317,077 (published May 24, 1989), which should detect sequences at the level of approximately 10.sup.6 /ml, utilizes nucleic acid multimers which bind to single-stranded analyte nucleic acid, and which also bind to a multiplicity of single-stranded labeled oligonucleotides. A particularly desirable technique may involve amplification of the target HCV sequences in sera approximately 10,000 fold (i.e., to approximately 10.sup.6 sequences/ml), as part of the hybridization system. The amplification may be accomplished, for example, by the polymerase chain reactions (PCR) technique described by Saiki et al. (1986), by Mullis, U.S. Pat. No. 4,683,195, and by Mullis et al. U.S. Patent No. 4,683,202. Amplification may be prior to, or preferably subsequent to purification of the HCV target sequence. For example, amplification may be utilized in conjunction with the assay methods described in U.S. Pat. No. 4,868,105, or if even further amplification is desired, in conjunction with the hybridization system described in EPO Publication No. 317,077.
Preferred methods for detecting HCV sequences in an analyte polynucleotide strand are based upon the hybridization detection methods described in U.S. Pat. No. 4,868,105 and in EPO Publication No. 317,077. These methods are solution-phase sandwich hybridization assays which utilize both capture and label probes which hybridize to target sequences in an analyte nucleic acid. In the use of these assays to screen biological samples for HCV, the probes used would bind to conserved regions of the HCV genome. The capture and label probes may be interspersed in their binding to the target sequence. Alternatively, in a preferred mode the capture and label probes are in sets, and the probes of one set do not intersperse with the probes of another set. In the latter mode, preferably the set(s) of multiple capture probes hybridize to the most conserved regions of the genome, while the set(s) of multiple label probes may hybridize to regions which exhibit small amounts of divergence. For example, using the prototype HCV1 CDNA sequence shown in FIG. 1, probes could be used which hybridize to sequences in the region of nucleotides from about -318 to about 174, and/or nucleotides in the region of about 4378 to about 4902, and/or nucleotides in the region of from about 4056 to about 4448. The preferred probes would hybridize to sequences in the 5'-region of the HCV genome, since, as shown infra., this region appears to be highly conserved. Thus, preferred probes may hybridize to, for example, nucleotides from about -318 to about 174 as shown in FIG. 1. Probes could be used which hybridize to either the positive strand in conserved regions, and/or its complement, depending upon the purpose, for example, to detect viral genomic sequences, or to detect HCV cDNA sequences resulting from PCR amplification, or to detect replicative intermediates to the positive HCV RNA strand.
Identification of RNA Which Hybridizes to HCV cDNA in infected Individuals
A. Identification of RNA in the Liver of an HCV-Infected Chimpanzee Which Hybridizes to HCV cDNA
RNA from the liver of a chimpanzee which had been infected with HCV was shown to contain a species of RNA which hybridized to the HCV cDNA contained within clone 81 by Northern blotting, as follows.
RNA (from a liver biopsy of a chimpanzee from which high titer plasma was derived) was isolated using techniques described in Maniatis et al (1982) for the isolation of total RNA from mammalian cells, and for its separation into poly A+ and poly A- fractions. These RNA fractions were subjected to electrophoresis on a formaldehyde/agarose gel (1% w/v), and transferred to nitrocellulose (Maniatis et al (1982)). The nitrocellulose filters were hybridized with radio-labeled HCV cDNA from clone 81 (see FIG. 5 for the nucleotide sequence of the insert.) To prepare the radio-labeled probe, the HCV cDNA insert isolated from clone 81 was radio-labeled with .sup.32 P by nick translation using DNA Polymerase I (Maniatis et al (1982)). Hybridization was for 18 hours at 42.degree. C. in a solution containing 10% (w/v) Dextran sulphate, 50% (w/v) deionized formamide, 750 mM NaCl, 75 mM Na citrate, 20 mM Na.sub.2 HPO.sub.4, pH 6.5, 0.1% SDS, 0.02% (w/v) bovine serum albumin (BSA), 0.02% (w/v) Ficoll-400, 0.02% (w/v) polyvinylpyrrolidone, 100 .mu.g/ml salmon sperm DNA which had been sheared by sonication and denatured, and 10.sup.6 CPM/ml of the nick-translated cDNA probe.
An autoradiograph of the probed filter is shown in FIG. 21. Lane 1 contains .sup.32 P-labeled restriction fragment markers. Lanes 2-4 contain chimpanzee liver RNA as follows: lane 2 contains 30 micrograms of total RNA; lane 3 contains 30 micrograms of poly A.sup.- RNA:, and lane 4 contains 20 micrograms of poly A.sup.+ RNA. As shown in FIG. 21, the liver of the HCV-infected chimpanzee contains a heterogeneous population of related poly A+ RNA molecules which hybridizes to the HCV cDNA probe, and which appears to be roughly greater than 5000 nucleotides in size. This RNA, which hybridizes to the HCV cDNA, could represent vital genomes and/or specific transcripts of the vital genome.
B. Identification of HCV-Derived RNA in Serum from Infected Individuals
Nucleic acids were extracted from particles isolated from high titer chimpanzee HCV plasma as follows. First, viral particles were isolated from the plasma; a 90 ml aliquot was diluted with 310 ml of a solution containing 50 mM Tris-HCl, pH 8.0, 1 mM EDTA, 100 mM NaCl. Debris was removed by centrifugation for 20 min at 15,000.times.g at 20.degree. C. Viral particles in the resulting supernatant were then pelleted by centrifugation in a Beckman SW28 rotor at 28,000 rpm for 5 hours at 20.degree. C. To release the vital genome, the particles were disrupted by suspending the pellets in 15 ml solution containing 1% sodium dodecyl sulfate (SDS), 10 mM EDTA, 10 mM Tris-HCl, pH 7.5, also containing 2 mg/ml proteinase K, followed by incubation at 45.degree. C. for 90 min. Nucleic acids were isolated by adding 0.8 .mu.g MS2 bacteriophage RNA as carrier, and extracting the mixture four times with a 1:1 mixture of phenol:chloroform (phenol saturated with 0.5M Tris-HCl, pH 7.5, 0.1% (v/v) beta-mercaptoethanol, 0.1% (w/v) hydroxyquinolone, followed by extraction two times with chloroform. The aqueous phase was concentrated with 1-butanol prior to precipitation with 2.5 volumes absolute ethanol overnight at -20.degree. C. Nucleic acid was recovered by centrifugation in a Beckman SW41 rotor at 40,000 rpm for 90 min at 4.degree. C., and dissolved in water that had been treated with 0.05% (v/v) diethylpyrocarbonate and autoclaved.
Aliquots (equivalent to 1 ml of original plasma) of the isolated nucleic acids were resuspended in 20 microliters 50 mM Hepes, pH 7.5, 1 mm EDTA and 16 micrograms/ml yeast soluble RNA. The samples were denatured by boiling for 5 minutes followed by immediate freezing, and were treated with RNase A (5 microliters containing 0.1 mg/ml RNase A in 25 mM EDTA, 40 mM Hepes, pH 7.5) or with DNase I (5 microliters containing 1 unit DNase I in 10 mM MgCl.sub.2, 25 mM Hepes, pH 7.5); control samples were incubated without enzyme. Following incubation, 230 microliters of ice-cold 2.times.SSC containing 2 micrograms/ml yeast soluble RNA was added, and the samples were filtered on a nitrocellulose filter. The filters were hybridized with a cDNA probe from clone 81, which had been .sup.32 P-labeled by nick-translation. FIG. 22 shows an autoradiograph of the filter. Hybridization signals were detected in the DNase treated and control samples (lanes 2 and 1, respectively), but were not detected in the RNase treated sample (lane 3). Thus, since RNase A treatment destroyed the nucleic acids isolated from the particles, and DNase I treatment had no effect, the evidence strongly suggests that the HCV genome is composed of RNA.
C. Characterization of the Strandedness of the HCV Genome
The HCV genome was characterized with respect to its strandedness by isolating the nucleic acid fraction from HCV captured on anti-HCV.sub.5-1-1 antibody coated polystyrene beads, and determining whether the isolated nucleic acid hybridized with plus and/or minus strands of HCV cDNA.
HCV was captured from HCV-infected chimpanzee plasma using polystyrene beads coated with immunopurified anti-HCV.sub.5-1-1 antibody as follows. Protein-nucleic acid complexes present in infectious plasma of a chimp with HCV were isolated Using purified human polyclonal anti-HCV antibodies which were bound to polystyrene beads. Polyclonal anti-HCV.sub.5-1-1 antibodies were purified from serum from a human with HCV using the SOD-HCV polypeptide encoded in clone 5-1-1. The purified anti-HCV.sub.5-1-1 antibodies were bound to polystyrene beads (1/4" diameter, specular finish, Precision Plastic Ball Co., Chicago, Ill.) by incubating each at room temperature overnight with 1 ml of antibodies (1 microgram/ml in borate buffered saline, pH 8.5). Following the overnight incubation, the beads were washed once with TBST �50 mM Tris HCl, pH 8.0, 150 mM NaCl, 0.05% (v/v) Tween 20!, and then with phosphate buffered saline (PBS) containing 10 mg/ml BSA. Control beads were prepared in an identical fashion, except that the purified anti-HCV.sub.5-1-1 antibodies were replaced with total human immunoglobulin.
An aliquot (1 ml) of the HCV-infected chimp plasma was incubated for 3 hours at 37.degree. C. with each of 5 beads coated with either anti-HCV.sub.5-1-1 antibodies, or with control immunoglobulins. The beads were washed 3 times with TBST. The washed beads were incubated for 60 min, at 37.degree. C. with 0.2 ml per bead of a solution containing proteinase K (1 mg/ml), 10 mM Tris HCl, pH 7.5, 10 mM EDTA, 0.25% (w/v) SDS, 10 micrograms/ml soluble yeast RNA, and the supernatant solution was removed. The supernatant was extracted with phenol and chloroform, and the nucleic acids precipitated with ethanol overnight at -20.degree. C. The nucleic acid precipitate was collected by centrifugation, dried, and dissolved in 50 mM Hepes, pH 7.5. Duplicate aliquots of the soluble nucleic acids from the samples obtained from beads coated with anti-HCV.sub.5-1-1 antibodies and with control beads containing total human immunoglobulin were filtered onto to nitrocellulose filters.
Aliquots of the isolated genomic nucleic acid equivalent to 3 mls of high titer plasma were blotted onto nitrocellulose filters. As controls, aliquots of denatured HCV cDNA from clone 81 (2 picograms) was also blotted onto the same filters. The filters were probed with .sup.32 P-labeled mixture of plus or mixture of minus strands of single stranded DNA cloned from HCV cDNAs; the cDNAs were excised from clones 40b, 81, and 25c.
The single stranded probes were obtained by excising the HCV cDNAs from clones 81, 40b, and 25c with EcoRI, and cloning the cDNA fragments in M13 vectors, mp18 and mp19 �Messing (1983)!. The M13 clones were sequenced to determine whether they contained the plus or minus strands of DNA derived from the HCV cDNAs. Sequencing was by the dideoxy chain termination method of Sanger et al. (1977).
Each of a set of duplicate filters containing aliquots of the HCV genome isolated from the captured particles was hybridized with either plus or minus strand probes derived from the HCV cDNAs. FIG. 23 shows the autoradiographs obtained from probing the HCV genome with the mixture of probes derived from clones 81, 40b, and 25c. This mixture was used to increase the sensitivity of the hybridization assay. The samples in panel I were hybridized with the plus strand probe mixture. The samples in panel II were probed by hybridization with the minus strand probe mixture. The composition of the samples in the panels of the immunoblot are presented in the following Table.
TABLE______________________________________lane A B______________________________________1 HCV genome *2 -- *3 * cDNA 814 -- cDNA 81______________________________________ * is an undescribed sample.
As seen from the results in FIG. 23, only the minus strand DNA probe hybridizes with the isolated HCV genome. This result, in combination with the result showing that the genome is sensitive to RNase and not DNase, suggests that the genome of NANBV is positive stranded RNA.
Detection of HCV RNA and Polynucleotides Derived Therefrom Using an HCV/cPCR Method
A particularly useful method for detecting HCV RNA or polynucleotides derived from HCV RNA is the HCV/cPCR method, which is a subject of the herein application, and which utilizes the polymerase chain reaction technique (PCR) which is described by Saiki et al. (1986), by Mullis in U.S. Pat. No. 4,683,195, and by Mullis et al. in U.S. Pat. No. 4,683,202. The HCV/cPCR method utilizes primers and probes derived from the information provided herein concerning the nature of the HCV genome.
Generally, in the PCR technique, short oligonucleotide primers are prepared which match opposite ends of a desired sequence. The sequence between the primers need not be known. A sample of polynucleotide is extracted and denatured, preferably by heat, and hybridized with oligonucleotide primers which are present in molar excess. Polymerization is catalyzed by a template- and primer-dependent polymerase in the presence of deoxynucleotide triphosphates or nucleotide analogs (dNTPs). This results in two "long products" which contain the respective primers at their 5'-termini, covalently linked to the newly synthesized complements of the original strands. The replicated DNA is again denatured, hybridized with oligonucleotide primers, returned to polymerizing conditions, and a second cycle of replication is initiated. The second cycle provides the two original strands, the two long products from cycle 1, and two "short products" replicated from the long products. The short products contain sequences (sense or antisense) derived from the target sequence, flanked at the 5'- and 3'-termini with primer sequences. On each additional cycle, the number of short products is replicated exponentially. Thus, this process causes the amplification of a specific target sequence.
In the method, a sample is provided which is suspected of containing HCV RNA, or a fragment thereof. The sample is usually taken from an individual suspected of having NANBH; however, other sources of the sample are included, e.g., conditioned medium or cells from in vitro systems in which the virus has been replicated. The sample, however, must contain the target nucleic acid sequence(s).
The sample is then subjected to conditions which allow reverse transcription of HCV RNA into HCV cDNA. Conditions for reverse transcribing RNA are known to those of skill in the art, and are described in, for example, Maniatis et al. (1982), and in Methods in Enzymology. A preferred method of reverse transcription utilizes reverse transcriptase from a variety of sources, including recombinant molecules, and isolated from, for example, a retrovirus, preferably from avian myeloblastosis virus (AMV), and suitable conditions for the transcription. The HCV cDNA product of reverse transcription is in a RNA:DNA hybrid, which results from the first round of reverse transcription; subsequently, DNA:DNA hybrids result from two or more rounds of transcription.
The HCV cDNA resulting from reverse transcription is then subjected to PCR to amplify the target sequence. In order to accomplish this, the HCV cDNA is denatured, and the separated strands are hybridized with primers which flank the target sequence.
Strand separation may be accomplished by any suitable denaturing method, including physical, chemical, or enzymatic means, which are known to those of skill in the art. A preferred method, which is physical, involves heating the nucleic acid until it is completely (>99%) denatured. Typical heat denaturation involves temperatures ranging from about 80.degree. C. to about 105.degree. C., for times ranging from about 1 to 10 minutes.
After hybridization of the HCV cDNA with the primers, the target HCV sequences are replicated by a polymerizing means which utilizes a primer oligonucleotide to initiate the synthesis of the replicate chain. The primers are selected so that they are complementary to sequences of the HCV genome. Oligomeric primers which are complementary to regions of the sense and antisense strands of HCV cDNA can be designed from the HCV cDNA sequences from the composite cDNA sequence provided in FIG. 1.
The primers are selected so that their relative positions along a duplex sequence are such that an extension product synthesized from one primer, when it is separated from its template (complement), serves as a template for the extension of the other primer to yield a replicate chain of defined length.
The primer is preferably single stranded for maximum efficiency in amplification, but may alternatively be double stranded. If double stranded, the primer is first treated to separate its strands before being used to prepare extension products. Preferably, the primer is an oligodeoxyribonucleotide. The primer must be sufficiently long to prime the synthesis of extension products in the presence of the agent for polymerization. The exact lengths of the primers will depend on many factors, including temperature and source of the primer and use of the method. For example, depending on the complexity of the target sequence, the oligonucleotide primer typically contains about 15-45 nucleotides, although it may contain more or fewer nucleotides. Short primer molecules generally require cooler temperatures to form sufficiently stable hybrid complexes with the template.
The primers used herein are selected to be "substantially" complementary to the different strands of each specific sequence to be amplified. Therefore, the primers need not reflect the exact sequence of the template, but must be sufficiently complementary to selectively hybridize with their respective strands. For example, a non-complementary nucleotide fragment may be attached to the 5'-end of the primer, with the remainder of the primer sequence being complementary to the strand. Alternatively, non-complementary bases or longer sequences can be interspersed into the primer, provided that the primer has sufficient complementarity with the sequence of one of the strands to be amplified to hybridize therewith, and to thereby form a duplex structure which can be extended by the polymerizing means. The non-complementary nucleotide sequences of the primers may include restriction enzyme sites. Appending a restriction enzyme site to the end(s) of the target sequence Would be particularly helpful for cloning of the target sequence.
It will be understood that "primer", as used herein, may refer to more than one primer, particularly in the case where there is some ambiguity in the information regarding the terminal sequence(s) of the target region to be amplified. Hence, a "primer" includes a collection of primer oligonucleotides containing sequences representing the possible variations in the sequence or includes nucleotides which allow a typical basepairing. One of the primer oligonucleotides in this collection will be homologous with the end of the target sequence. A specific case is shown in the Examples, where oligomer sets of 44-mers and 45-mers were utilized to prime the amplification of a potentially variant region of the HCV genome.
It is anticipated that there will be a variety of strains or isolates of HCV with sequences which deviate from HCV1, the prototype strain. Therefore, in order to detect variant Strains it is preferable to construct primers which hybridize to conserved regions of the HCV genome. The conserved regions may be determined by comparing the nucleotide or amino acid sequences of several HCV strains/isolates. There appear to be at least three regions of conserved amino acid in the HCV genome, described supra., from which primers may be derived. These regions are believed to be. The primers described infra., in the Examples, are derived from what are believed to be conserved regions of HCV, based upon sequence homology to that of the Flaviviruses.
The oligonucleotide primers may be prepared by any suitable method. Methods for preparing oligonucleotides of specific sequence are known in the art, and include, for example, cloning and restriction of appropriate sequences, and direct chemical synthesis. Chemical synthesis methods may include, for example, the phosphotriester method described by Narang et al. (1979), the phosphodiester method disclosed by Brown et al. (1979), the diethylphosphoramidate method disclosed in Beaucage et al. (1981), and the solid support method in U.S. Pat. No. 4,458,066.
The primers may be labeled, if desired, by incorporating means detectable by spectroscopic, photochemical, biochemical, immunochemical, or chemical means.
Template-dependent extension of the oligonucleotide primer(s) is catalyzed by a polymerizing agent in the presence of adequate amounts of the four deoxyribonucleotide triphosphates (dATP, dGTP, dCTP and dTTP) or analogs, in a reaction medium which is comprised of the appropriate salts, metal cations, and pH buffering system. Suitable polymerizing agents are enzymes known to catalyze primer- and template-dependent DNA synthesis. Known DNA polymerases include, for example, E. coli DNA polymerase I or its Klenow fragment, T.sub.4 DNA polymerase, and Taq DNA polymerase. The reaction conditions for catalyzing DNA synthesis with these DNA polymerases are known in the art.
The products of the synthesis are duplex molecules consisting of the template strands and the primer extension strands, which include the target sequence. These products, in turn, serve as template for another round of replication. In the second round of replication, the primer extension strand of the first cycle is annealed with its complementary primer; synthesis yields a "short" product which is bounded on both the 5'- and the 3'-ends by primer sequences or their complements. Repeated cycles of denaturation, primer annealing, and extension result in the exponential accumulation of the target region defined by the primers. Sufficient cycles are run to achieve the desired amount of polynucleotide containing the target region of nucleic acid. The desired amount may vary, and is determined by the function which the product polynucleotide is to serve.
The PCR method can be performed in a number of temporal sequences. For example, it can be performed step-wise, where after each step new reagents are added, or in a fashion where all of the reagents are added simultaneously, or in a partial step-wise fashion, where fresh reagents are added after a given number of steps.
In a preferred method, the PCR reaction is carried out as an automated process which utilizes a thermostable enzyme. In this process the reaction mixture is cycled through a denaturing region, a primer annealing region, and a reaction region. A machine may be employed which is specifically adapted for use with a thermostable enzyme, which utilizes temperature cycling without a liquid handling system, since the enzyme need not be added at every cycle. This type of machine is commercially available from Perkin Elmer Cetus Corp.
After amplification by PCR, the target polynucleotides are detected by hybridization with a probe polynucleotide which forms a stable hybrid with that of the target sequence under stringent to moderately stringent hybridization and wash conditions. If it is expected that the probes will be completely complementary (i.e., about 99% or greater) to the target sequence, stringent conditions will be used. If some mismatching is expected, for example if variant strains are expected with the result that the probe will not be completely complementary, the stringency of hybridization may be lessened. However, conditions are chosen which rule out nonspecific/adventitious binding. Conditions which affect hybridization, and which select against nonspecific binding are known in the art, and are described in, for example, Maniatis et al. (1982). Generally, lower salt concentration and higher temperature increase the stringency of binding. For example, it is usually considered that stringent conditions are incubation in solutions which contain approximately 0.1.times.SSC, 0.1% SDS, at about 65.degree. C. incubation/wash temperature, and moderately stringent conditions are incubation in solutions which contain approximately 1-2.times.SSC, 0.1% SDS and about 50.degree.-65.degree. C. incubation/wash temperature. Low stringency conditions are 2.times.SSC and about 30.degree.-50.degree. C.
Probes for HCV target sequences may be derived from the HCV cDNA sequence shown in FIG. 1, or from new HCV isolates. The HCV probes may be of any suitable length which span the target region, but which exclude the primers, and which allow specific hybridization to the target region. If there is to be complete complementarity, i.e., if the strain contains a sequence identical to that of the probe, since the duplex will be relatively stable under even stringent conditions, the probes may be short, i.e., in the range of about 10-30 base pairs. If some degree of mismatch is expected with the probe, i.e., if it is suspected that the probe will hybridize to a variant region, the probe may be of greater length, since length seems to counterbalance some of the effect of the mismatch(es). An example of this is found in the Examples, where the probe was designed to bind to potential variants of HCV1. In this case, the primers were designed to bind to HCV cDNA derived from a hypothetical conserved region of the HCV genome, and the target region was one which potentially contained variations (based upon the Flavivirus model). The probe used to detect the HCV target sequences Contained approximately 268 base pairs.
The probe nucleic acid having a sequence complementary to the target sequence may be synthesized using similar techniques described supra. for the synthesis of primer sequences. If desired, the probe may be labeled. Appropriate labels are described supra.
In some cases, it may be desirable to determine the length of the PCR product detected by the probe. This may be particularly true if it is suspected that variant HCV strains may contain deletions within the target region, or if one wishes to confirm the length of the PCR product. In such cases it is preferable to subject the products to size analysis as well as hybridization with the probe. Methods for determining the size of nucleic acids are known in the art, and include, for example, gel electrophoresis, sedimentation in gradients, and gel exclusion chromatography.
The presence of the target sequence in a biological sample is detected by determining whether a hybrid has been formed between the HCV polynucleotide probe and the nucleic acid subjected to the PCR amplification technique. Methods to detect hybrids formed between a probe and a nucleic acid sequence are known in the art. For example, for convenience, an unlabeled sample may be transferred to a solid matrix to which it binds, and the bound sample subjected to conditions which allow specific hybridization with a labeled probe; the solid matrix is than examined for the presence of the labeled probe. Alternatively, if the sample is labeled, the unlabeled probe is bound to the matrix, and after the exposure to the appropriate hybridization conditions, the matrix is examined for the presence of label. Other suitable hybridization assays are described supra.
Determination of Variant HCV Sequences Using PCR
In order to identify variant HCV strains, and thereby to design probes for those variants, the above described HCV/cPCR method is utilized to amplify variant regions of the HCV genome, so that the nucleotide sequences of these variant target regions can be determined. Generally, variant types of HCV might be expected to occur in different geographic locations than that in which the HCV1 strain is predominant, for example, Japan, Africa, etc.; or in different vertebrate species which are also infected with the virus. Variant HCV may also arise during passage in tissue culture systems, or be the result of spontaneous or induced mutations.
In order to amplify the variant target region, primers are designed to flank the suspect region, and preferably are complementary to conserved regions. Primers to two regions of HCV which are probably conserved, based upon the Flavivirus model, are described in the Examples. These primers and probes may be designed utilizing the sequence information for the HCV1 strain provided in FIG. 1.
Analysis of the nucleotide sequence of the target region(s) may be by direct analysis of the PCR amplified products. A process for direct sequence analysis of PCR amplified products is described in Saiki et al. (1988).
Alternatively, the amplified target sequence(s) may be cloned prior to sequence analysis. A method for the direct cloning and sequence analysis of enzymatically amplified genomic segments has been described by Scharf (1986). In the method, the primers used in the PCR technique are modified near their 5'-ends to produce convenient restriction sites for cloning directly into, for example, an M13 sequencing vector. After amplification, the PCR products are cleaved with the appropriate restriction enzymes. The restriction fragments are ligated into the. M13 vector, and transformed into, for example, a JM 103 host, plated out, and the resulting plaques are screened by hybridization with a labeled oligonucleotide probe. Other methods for cloning and sequence analysis are known in the art.
Universal Primers for Flaviviruses and for HCV
Studies of the nature of the genome of the HCV, utilizing probes derived from the HCV cDNA, as well as sequence information contained within the HCV cDNA, are suggestive that HCV is a Flavi-like virus. These studies are described in U.S. Pat. No. 5,350,671 owned by the herein assignee, and which is incorporated herein in its entirety. A comparison of the HCV cDNA sequence derived from the HCV cDNA clones with known sequences of a number of Flaviviruses show that HCV contains sequences which are homologous to conserved sequences in the Flaviviruses. These conserved sequences may allow the creation of primers which may be universal in their application for amplification of target regions of Flaviviruses, and for HCV. These sequences are the 16-mer or smaller sequences from the 3'-termini of the primers described in the Examples. Identification of the species is then accomplished utilizing a probe specific for the species. The genomes of a number of Flaviviruses are known in the art, and include, for example, Japanese Encephalitis Virus (Sumiyoshi et al. (1987)), Yellow Fever Virus (Rice et al. (1985)), Dengue Type 2 Virus (Hahn et al. (1988)), Dengue Type 4 Virus (Mackow (1987)), and West Nile Virus (Castle et al. (1986)). Identification of HCV RNA is accomplished utilizing a probe specific for HCV, the sequence of which can be determined the HCV cDNA sequences provided herein.
Alternatively, utilization of sets of probe(s) designed to account for codon degeneracy and therefore contain common sequences to the Flaviviruses and to HCV, as determined by a comparison of HCV amino acid sequences with the known sequences of the Flaviviruses, allows a general detection system for these viruses.
Construction of Desired DNA Sequences
Synthetic oligonucleotides may be prepared using an automated oligonucleotide Synthesizer as described by Warner (1984). If desired the synthetic strands may be labeled with .sup.32 P by treatment with polynucleotide kinase in the presence of .sup.32 P-ATP, using standard conditions for the reaction.
DNA sequences, including those isolated from cDNA libraries, may be modified by known techniques, including, for example site directed mutagenesis, as described by Zoller (1982). Briefly, the DNA to be modified is packaged into phage as a single stranded sequence, and converted to a double stranded DNA with DNA polymerase using, as a primer, a synthetic oligonucleotide complementary to the portion of the DNA to be modified, and having the desired modification included in its own sequence. The resulting double stranded DNA is transformed into a phage supporting host bacterium. Cultures of the transformed bacteria, which contain replications of each strand of the phage, are plated in agar to obtain plaques. Theoretically, 50% of the new plaques contain phage having the mutated sequence, and the remaining 50% have the original sequence. Replicates of the plaques are hybridized to labeled synthetic probe at temperatures and conditions which permit hybridization with the correct strand, but not with the unmodified sequence. The sequences which have been identified by hybridization are recovered and cloned.
Kits for Screening for HCV Derived Polynucleotides
Oligomers which are probes and/or primers for amplification and/or screening of samples for HCV can be packaged into kits. Kits for screening for HCV sequences include the oligomeric probe DNAs. Kits for amplification of HCV sequences may include the oligomeric primers used in the amplification. The kits usually contain the probes or primers in a premeasured or predetermined amount, as well as other suitably packaged reagents and materials, in separate suitable containers, needed for the particular hybridization and/or amplification protocol(s). For example, the kit may contain standards, buffers, supports, enzymes, substrates, label probes, binding partners, and/or instructions for conducting the test.
EXAMPLES
Described below are examples of the present invention which are provided only for illustrative purposes, and not to limit the scope of the present invention.
Isolation and Sequence of Clone 5'-clone32
A clone containing sequence from the 5'-region of the HCV genome, upstream of the sequence in clone b114a, was isolated and the nucleotide sequence determined by a modification Of the method for the isolation and sequence of clones generated by PCR amplification of the 3'-region of the genome, described in U.S. Pat. No. 5,350,671 which is incorporated by reference. Generally, a target region of the genome was amplified by the PCR technique described in Saiki et al. (1986), and in Saiki et al (1988). The HCV RNA which was amplified was obtained by extracting human serum (U.S. clinical isolate, HCV27) using a cold guanidinium thiocyanate method described by Han et al. (1987). The extracted RNA was converted into single stranded cDNA with reverse transcriptase, using a primer, JH94, which is complementary to nucleotides -250 to -223 of the HCV genome (see FIG. 1). The sequence of JH94 is:
5' CCT GCG GCC GCA CGA CAC TCA TAC TAA 3'.
Conversion of single- to double-stranded HCV cDNA was accomplished by tailing the DNA with approximately 20 to 50 dA residues using terminal deoxynucleotidyl transferase (Sambrook et al. (1989), MOLECULAR CLONING), and replicating the tailed molecule using the following oligo-dT primer-adapter, which contains a NotI site, and an sp6 promoter:
__________________________________________________________________________Stuffer NotI SP6 Promoter Primer__________________________________________________________________________AATTC GCGGCCGC CATACGATTTAGGTGACACTATAGAA T.sub.15__________________________________________________________________________
The resultant cDNA was subjected to amplification by PCR using two primers, JH94 (described supra.) and JH11, which has the following sequence.
______________________________________Primer Sequence______________________________________JH11 (20mer) AATTCGGGCGGCCGCCATACGA______________________________________
The PCR reaction was carried out by suspending the cDNA and the primers in 100 microliters of reaction mixture containing the four deoxynucleoside triphosphates, buffer salts and metal ions, and a thermostable DNA polymerase isolated from Thermus aquaticus (Taq polymerasea, which are in a Perkin Elmer Cetus PCR kit (N801-0043 or N801-0055). The PCR reaction was performed for 35 cycles in a Perkin Elmer Cetus DNA thermal cycler. Each cycle consisted of a 1.5 min denaturation step at 94.degree. C., an annealing step at 60.degree. C. for 2 min, and a primer extension step at 72.degree. C. for 3 min.
The PCR products were digested with NotI, and cloned into pUC18S. Clones containing HCV nucleotide sequences were obtained by screening with a probe, Alex90, which is derived from nucleotides -312 to -283 of the HCV1 genome, and which has the sequence:
5' ACC ATG AAT CAC TCC CCT GTG AGG AAC TAC 3'.
The HCV cDNAs in the isolated clones were sequenced by the dideoxy chain termination method (Sanger et al. (1977)). The sequence of HCV cDNA in one of the isolated clones, 5'-clone32, spans the region of nucleotides -224 to -341 in FIG. 1.
An analysis of the nucleotide sequence of the HCV cDNA showed that the replicate of the HCV RNA strand contains a GC-rich stretch which may be capable of forming a stable hairpin structure: ##STR1## In the structure, the dashed lines indicate possible hydrogen bonds between complementary nucleotides.
A search in the computer database, Genebank, revealed that homologous sequences were absent from known viral sequences. Thus, this sequence may be unique to the 5'-terminus of the HCV genome.
A hairpin structure may serve as a recognition signal for a transcriptase and/or it may contribute to the stability of the RNA at the 5'-terminus.
Compiled HCV cDNA Sequences
An HCV cDNA sequence has been compiled from a series of overlapping clones derived from various HCV cDNA libraries described in U.S. Pat. NO. 5,350,671, to which has been added the sequence of 5'-clone32. The other clones from which FIG. 1 has been derived are b114a, 18g, ag30a, CA205a, CA290a, CA216a, pi14a, CA167b, CA156e, CA84a, CA59a, K9-1 (also called k9-1), 26j, 13i, 12f, 14i, 11b, 7f, 7e, 8h, 33c, 40b, 37b, 35, 36, 81, 32, 33b, 25c, 14c, 8f, 33f, 33g, 39c, 35f, 19g, 26g, 15e, b5a, 16jh, C6k and p131jh. The methods for isolation of these clones, as well as their sequences, are discussed in U.S. Ser. No. 07/456,637, which is incorporated herein by reference. In FIG. 1, the three dashes above the sequence indicate the position of the putative initiator methionine codon.
Clone b114a overlaps with clones 18g, ag30a, and CA205a, except that clone b114a contains an extra two nucleotides upstream of the sequence in clone 18g (i.e., 5'-CA). These extra two nucleotides have been included in the HCV genomic sequence shown in FIG. 1.
It should be noted that although several of the clones described supra. have been obtained from libraries other than the original HCV cDNA lambda-gt11 C library (ATCC No. 40394), these clones contain HCV cDNA sequences which overlap HCV cDNA sequences in the original library. Thus, essentially all of the HCV sequence is derivable from the original lambda-gt11 C library (ATCC No. 40394) which was used to isolate the first HCV cDNA clone (5-1-1). The isolation of clone 5-1-1 is described in U.S. Pat. No. 5,350,671 which is incorporated herein by reference.
The putative sequence of the major HCV polyprotein encoded in the composite of HCV1 cDNA is also shown. The first amino acid in the sequence is the putative initiator methionine of the large ORF. The variant amino acids, due to the clonal heterogeneities, are indicated above the sequence. Since the lambda gt11 library was created from serum obtained from one individual (see U.S. Pat. No. 5,350,671), the results suggest that variant viral sequences (both nucleotide and amino acid) are present in that individual.
An examination of the composite HCV cDNA sequence shows that besides the large ORF, there are a number of ORFs upstream of that encoding the polyprotein, and within the sequence encoding the polyprotein there are a large number of smaller ORFs in the other two translational frames. The ORFs upstream of the HCV polyprotein are shown in the Table immediately below.
TABLE______________________________________ORFs Upstream of that Encoding the LargeHCV PolyproteinNucl. # Translation Frame Amino Acid Sequence______________________________________-310 1 MNHSPVRNYCLHAESV-329 3 MGATLHHESLPCEELL SSRRKRLAMALV-246 2 MSVVQPPGPPLPGEP-127 1 MPGDLGVPPQDC______________________________________
The reading frame, position, and size of the ORFs downstream of the sequence encoding the putative initiator MET of the polyprotein are shown in the Table below. The major polyprotein is that translated from reading frame 2.
TABLE______________________________________ORFs Downstream of the Putative Initiator METEncoding SequenceReading Frame Size(aa) Position(bp)______________________________________1 168 6961 105 23431 119 56162 3025 -423 160 53 111 16673 148 6893______________________________________
In addition to the above, an examination of the sequence which is complementary to the genomic strand of HCV RNA also contains several small ORFs. One of these ORFs, which is complementary to nucleotides -341 to +837 in the HCV RNA sequence, encodes a polypeptide of 385 amino acids.
Comparison of the Sequences of 5'-Regions Obtained from HCV Isolates from Different Geographical Locations
Nucleotide sequences from the 5'- regions of HCV isolates from the U.S.A. (HCV18, HCV27), from Italy (HCVI1, HCVI24), and from Korea (HCVK1) were compared.
Isolation of the HCV cDNA sequences was essentially as described supra., for the isolation of 5'-clone32, except for the following. The extracted RNA was reverse-transcribed into cDNA using as primers either JH51 or r16, which are complementary to HCV nucleotides -90 to -73 and 366 to 383, respectively. The sequences of these primers are as follows.
______________________________________Primer Sequence______________________________________JH51 5' CCC AAC ACT ACT CGG CTA 3'r16 5' CAC GTA AGG GTA TCG ATG 3'______________________________________
Amplification of the HCV dsDNA was by the PCR method using JH93 and JH52 as 5'- and 3'- primers, respectively. The HCV sequence in JH93 is derived from HCV nucleotides -317 to -296, that in JH52 is from HCV nucleotides -93 to -117; the nucleotide numbers are indicated in parentheses below the sequences. In JH52 the underlined dinucleotide has been mutated to create the NotI site. The sequences of these primers are the following.
__________________________________________________________________________(Primer) Stuffer NotI HCV sequence__________________________________________________________________________(JH93) 5' TTC GCGGCCGC ACTCCATGAATCACTCCCC 3' (-317) (-296)(JH52) 5' AGTCTT GCGGCCGC ACGCCCAAATC 3' (-93) (-117)__________________________________________________________________________
After amplification, the PCR products were cleaved by NotI, and cloned into pUC18S. The HCV cDNAs were sequenced either by direct sequencing after amplification by PCR, or alternatively, the cloned HCV cDNAs were sequenced by the primer extension and the dideoxy method. Primer extension and the dideoxy method of sequencing were performed as described supra., for the sequence of 5'-clone32.
The PCR method for direct sequencing used Alex90 (see supra. for the sequence) as the 5'-primer, and r25 as the 3'-primer. Alex90 is derived from HCV nucleotides -312 to -283, and r25 is derived from nucleotides 365 to 342 (See FIG. 1). The sequence of r25 is:
5' ACC TTA CCC AAA TTG CGC GAC CTA 3'.
A comparison of the sequences of the 5'-region of HCV27, HCVK1, HCVI1, HCVI24, and HCV18 with the sequence of the prototype HCV, HCV1, showed the following. The examined 5'- region is highly conserved amongst the 5 HCV isolates. The sequences appeared to be identical except for one nucleotide which was deleted at position -171 in HCVI24, and for the ambiguity in four nucleotides at positions -222 to -219 in isolate HCVK1.
The high levels of sequence conservation in this region may reflect the role of this region in viral replication, and/or transcription, and/or translation.
Detection of Positive and Negative Strand 5'-HCV RNA in Serum
The RNA in HCV27, isolated from serum, was analyzed for the presence of positive and negative strands using the PCR method. The PCR method was performed essentially as described above, except for the following. The extracted HCV27 RNA was reverse transcribed into single-stranded cDNA using as a primer either Alex90 or JH52 (see supra. for the sequences). The sequence of Alex90 matches that in nucleotides -312 to -283 of the positive strand of HCV RNA, whereas JH52 matches that of nucleotides -117 to -93 of the negative strand the resulting single-stranded HCV cDNAs were each separately amplified by PCR using Alex90 and JH52. Detection of the amplified products was accomplished by Southern blotting, using Alex89 as the probe. Alex89 matches nucleotide numbers -203 to -175 of HCV RNA. The sequence of Alex89 is:
5' CCA TAG TGG TCT GCG GAA CCG GTG AGT ACA 3'.
The analysis indicated that, by this method, the signals of the amplified products of both RNA strands were of equal intensity. These results are suggestive that HCV RNA in the 5'-region may exist as double-stranded RNA.
Probes for Sandwich Hybridization for HCV
This example exemplifies the sets of label and capture probes useful to detect HCV RNA in biological samples, using essentially the assay described in U.S. Pat. No. 4,868,105. The method is a solution-phase sandwich hybridization assay which utilizes both capture and label probes which hybridize to target sequences in an analyte nucleic acid. In the screening of biological samples for HCV, the probes used bind to conserved regions of the HCV genome, and the HCV binding regions are selected for their uniqueness to the HCV genome. The regions which bind to the binding partner of the capture probe, or the portion of the label probe which binds to the labeling moiety (or to an amplifying multimer if the method described in EPO Publication No. 317,077 is used), are selected such that they do not bind to any of the known sequences in the databank or in HCV, and which have the appropriate content of Gs and Cs to allow stable duplex formation with their complements under the selection conditions. The capture and label probes are in sets, and the probes of one set do not intersperse with the probes of another set. These probes are comprised of sequences which are complementary to the following nucleotide sequences in the coding strand of the prototype HCV cDNA sequence shown in FIG. 1.
______________________________________ Complement ofProbe type Probe Number Nucleotide Numbers______________________________________Capture 42.XT1.1 -318 to -289Capture 42.XT1.2 -285 to -256Capture 42.XT1.3 -252 to -223Capture 42.XT1.4 -219 to -190Label 42.LLA2C.5 -186 to -157Label 42.LLA2C.6 -153 to -124Label 42.LLA2C.7 -120 to -91Label 42.LLA2C.8 -87 to -58Label 42.LLA2C.9 -54 to -25Label 42.LLA2C.10 -21 to 9Label 42.LLA2C.11 13 to 42Label 42.LLA2C.12 46 to 75Label 42.LLA2C.13 79 to 108Label 42.LLA2C.14 112 to 141Label 42.LLA2C.15 145 to 174______________________________________
______________________________________ Complement ofProbe type Probe Number Nucleotide Numbers______________________________________Capture 42.16.XT1 4378 to 4407Capture 42.17.XT1 4411 to 4440Capture 42.18.XT1 4444 to 4473Capture 42.19.XT1 4477 to 4506Capture 42.20.XT1 4510 to 4539Label 42.21.LLA2C 4543 to 4572Label 42.22.LLA2C 4576 to 4605Label 42.23.LLA2C 4609 to 4638Label 42.24.LLA2C 4642 to 4671Label 42.25.LLA2C 4675 to 4704Label 42.26.LLA2C 4708 to 4737Label 42.27.LLA2C 4771 to 4770Label 42.28.LLA2C 4774 to 4803Label 42.29.LLA2C 4807 to 4836Label 42.30.LLA2C 4840 to 4869Label 42.31.LLA2C 4873 to 4902______________________________________
______________________________________ Complement ofProbe type Probe Number Nucleotide Numbers______________________________________Capture 42.32.XT1 4056 to 4085Capture 42.33.XT1 4089 to 4085Capture 42.34.XT1 4122 to 4151Capture 42.35.XT1 4155 to 4184Label 42.36.LLA2C 4188 to 4217Label 42.37.LLA2C 4221 to 4250Label 42.38.LLA2C 4254 to 4283Label 42.39.LLA2C 4287 to 4316Label 42.40.LLA2C 4230 to 4349Label 42.41.LLA2C 4353 to 4382Label 42.42.LLA2C 4386 to 4415Label 42.43.LLA2C 4419 to 4448______________________________________
In the above sets, each capture probe contains, in addition to the sequences complementary to the HCV sequences, the following sequence downstream of the HCV sequence (i.e., at the 3'-end):
5' CTT CTT TGG AGA AAG TGG TG 3'.
The sequence common to each capture probe is complementary to a sequence in the binding, partner(s), so that after hybridization, the duplex can be captured via affixation to the solid phase.
Also, in each set, each label probe contains, in addition to the sequences complementary to the HCV sequences, the following sequence downstream of the HCV sequence:
5' TTA GGC ATA GGA CCC GTG TC 3'.
If the method described in EPO Publication No. 317,077 is used, the sequence common to each label probe is complementary to a sequence in a multimer, to allow hybrid duplex formation with that multimer.
The sequences of the probes in the above sets are shown in FIG. 2.
Detection of HCV Polynucleotide Sequences Using PCR Amplification
The generalized method for amplification of HCV RNA by cPCR is shown in FIG. 4. In the diagram it is contemplated that the RNA strand is a virion or mRNA strand, which is a "sense" strand. However, it is also possible that replicative intermediate forms may also be detected which would be "anti-sense"; in this case the primer would be "sense". An RNA sense strand containing the target region is hybridized with an anti-sense primer which primes the synthesis of the replicate strand containing the target. cDNA to the RNA template is synthesized with a primer- and template-dependent reverse transcriptase. The cDNA in the resulting RNA:cDNA hybrid is released by denaturation and treatment with RNAse. Primers are annealed to the cDNA, and extended with a primer- and template-dependent DNA polymerase. The products are denatured, re-annealed to primers, and a second round of synthesis is conducted. A number of cycles are run until the amplified product containing the target region is in a desired amount, which is at least a detectable level.
Detection of Amplified HCV Nucleic Acid Sequences derived from HCV Nucleic Acid Sequences in Liver and Plasma Specimens from Chimpanzees with NANBH
HCV nucleic acids present in liver and plasma of chimpanzees with NANBH, and not in control chimpanzees, were amplified using essentially the polymerase chain reaction (PCR) technique described by Saiki et al. (1986). The primer oligonucleotides were derived from the HCV cDNA sequences in clone 81, or clones 36 and 37b. The amplified sequences were detected by gel electrophoresis and a modified Southern blotting method, using as probes the appropriate cDNA oligomer or nick-translated cDNA sequence with a sequence from the region between, but not including, the two primers.
Samples of RNA containing HCV sequences to be examined by the amplification system were isolated from liver biopsies of three chimpanzees with NANBH, and from two control chimpanzees. The isolation of the poly A.sup.+ RNA fraction was by the guanidinium thiocyanate procedure described in Maniatis et al. (1982).
Samples of RNA which were to be examined by the amplification system were also isolated from the plasmas of two chimpanzees with NANBH, and from one control chimpanzee, as well as from a pool of plasmas from control chimpanzees. One infected chimpanzee had a titer equal to or greater than 10.sup.6 CID/ml, and the other infected chimpanzee had a titer equal to or greater than 10.sup.5 CID/ml.
The nucleic acids were extracted from the plasma as follows. Either 0.1 ml or 0.01 ml of plasma was diluted to a final volume of 1.0 ml, with a TENB/proteinase K/SDS solution (0.05M Tris-HCL, pH 8.0, 0.001M EDTA, 0.1M NaCl, 1 mg/ml Proteinase K, and 0.5% SDS) containing 10 micrograms/ml polyadenylic acid, and incubated at 37.degree. C. for 60 minutes. After this proteinase K digestion, the resultant plasma fractions were deproteinized by extraction with TE (10.0 mM Tris-HCl, pH 8.0, 1 mM EDTA) saturated phenol. The phenol phase was separated by centrifugation, and was reextracted with TENB containing 0.1% SDS. The resulting aqueous phases from each extraction were pooled, and extracted twice with an equal volume of phenol/chloroform/isoamyl alcohol �1:1(99:1)!, and then twice with an equal volume of a 99:1 mixture of chloroform/isoamyl alcohol. Following phase separation by centrifugation, the aqueous phase was brought to a final concentration of 0.2M Na Acetate, and the nucleic acids were precipitated by the addition of two volumes of ethanol. The precipitated nucleic acids were recovered by ultracentrifugation in a SW 41 rotor at 38K, for 60 minutes at 4.degree. C.
In addition to the above, the high titer chimpanzee plasma and the pooled control plasma alternatively were extracted with 50 micrograms of poly A carrier by the procedure of Chomcyzski and Sacchi (1987). This procedure uses an acid guanidinium thiocyanate extraction. RNA was recovered by centrifugation at 10,000 RPM for 10 minutes at 4.degree. C. in an Eppendorf microfuge.
On two occasions, prior to the synthesis of cDNA in the PCR reaction, the nucleic acids extracted from plasma by the proteinase K/SDS/phenol method were further purified by binding to and elution from S and S Elutip-R Columns. The procedure followed was according to the manufacturer's directions.
The cDNA used as a template for the PCR reaction was derived from the nucleic acids (either total nucleic acids or RNA) prepared as described above. Following ethanol precipitation, the precipitated nucleic acids were dried, and resuspended in DEPC treated distilled water. Secondary structures in the nucleic acids were disrupted by heating at 65.degree. C. for 10 minutes, and the samples were immediately cooled on ice. cDNA was synthesized using 1 to 3 micrograms of total chimpanzee RNA from liver, or from nucleic acids (or RNA) extracted from 10 to 100 microliters of plasma. The synthesis utilized reverse transcriptase, and was in a 25 microliter reaction, using the protocol specified by the manufacturer, BRL. The primers for cDNA synthesis were those also utilized in the PCR reaction, described below. All reaction mixtures for cDNA Synthesis contained 23 units of the RNAase inhibitor, RNASIN.TM. (Fisher/Promega). Following cDNA Synthesis, the reaction mixtures were diluted with water, boiled for 10 minutes, and quickly chilled on ice.
The PCR reactions were performed essentially according to the manufacturer's directions (Cetus-Perkin-Elmer), except for the addition of 1 microgram of RNase A. The reactions were carried out in a final volume of 100 microliters. The PCR was performed for 35 cycles, utilizing a regimen of 37.degree. C. (2 min), 72.degree. C. (3 min), and 94.degree. C. (1 min).
The primers for cDNA synthesis and for the PCR reactions were derived from the HCV cDNA sequences in either clone 81, clone 36, or Clone 37b. (The HCV cDNA sequences of clones 81, 36, and 37b are shown in FIGS. 5, 6, and 7, respectively.) The sequences of the two 16-mer primers derived from clone 81 were:
5' CAA TCA TAC CTG ACA G 3'
and
5' GAT AAC CTC TGC CTG A 3'.
The sequence of the primer from clone 36 was:
5' GCA TGT CAT GAT GTA T 3'.
The sequence of the primer from clone 37b was:
5' ACA ATA CGT GTG TCA C 3'.
In the PCR reactions, the primer pairs consisted of either the two 16-mers derived from clone 81, or the 16-mer from clone 36 and the 16-mer from clone 37b.
The PCR reaction products were analyzed by separation of the products by alkaline gel electrophoresis, followed by Southern blotting, and detection of the amplified HCV-cDNA sequences with a .sup.32 p-labeled internal oligonucleotide probe derived from a region of the HCV cDNA which does not overlap the primers. The PCR reaction mixtures were extracted with phenol/chloroform, and the nucleic acids precipitated from the aqueous phase with salt and ethanol. The precipitated nucleic acids were collected by centrifugation, and dissolved in distilled water. Aliquots of the samples were subjected to electrophoresis on 1.8% alkaline agarose gels. Single stranded DNA of 60, 108, and 161 nucleotide lengths were co-electrophoresed on the gels as molecular weight markers. After electrophoresis, the DNAs in the gel were transferred onto Biorad Zeta Probe.TM. paper. Prehybridization and hybridization, and wash conditions were those specified by the manufacturer (Biorad).
The probes used for the hybridization-detection of amplified HCV cDNA sequences were the following. When the pair of PCR primers were derived from clone 81, the probe was an 108-mer with a sequence corresponding to that which is located in the region between the sequences of the two primers. When the pair of PCR primers were derived from clones 36 and 37b, the probe was the nick-translated HCV cDNA insert derived from clone 35. The primers are derived from nucleotides 155-170 of the clone 37b insert, and 206-268 of the clone 36 insert. The 3'-end of the HCV cDNA insert in clone 35 overlaps nucleotides 1-186 of the insert in clone 36; and the 5'-end of clone 35 insert overlaps nucleotides 207-269 of the insert in clone 37b. (Compare FIGS. 6, 17 and 7.) Thus, the cDNA insert in clone 35 spans part of the region between the sequences of the clone 36 and 37b derived primers, and is useful as a probe for the amplified sequences which include these primers.
Analysis of the RNA from the liver specimens was according to the above procedure utilizing both sets of primers and probes. The RNA from the liver of the three chimpanzees with NANBH yielded positive hybridization results for amplification sequences of the expected size (161 and 586 nucleotides for 81 and 36 and 37b, respectively), while the control chimpanzees yielded negative hybridization results. The same results were achieved when the experiment was repeated three times.
Analysis of the nucleic acids and RNA from plasma was also according to the above procedure utilizing the primers and probe from clone 81. The plasmas were from two chimpanzees with NANBH, from a control chimpanzee, and pooled plasmas from control chimpanzees. Both of the NANBH plasmas contained nucleic acids/RNA which yielded positive results in the PCR amplified assay, while both of the control plasmas yielded negative results. These results have been repeatedly obtained several times.
Defective viruses have been known to occur in RNA viruses. By using PCR technology it is possible to design primers to amplify sequences of the HCV genome. By analysis of the amplified products, it is expected to be able to identify both defective versions of the viral genome as well as wild-type viral species. Accordingly, using two primers based on known HCV sequence, one can predict accurately the expected size of the PCR product. Any larger species observed by gel electrophoresis and hybridization analysis could represent potential variant genomes. Alternatively, any smaller species observed in this fashion might represent defective agents. Analyses of these types would be useful in confirming the exact origin of the known HCV sequence, whether it is indeed a wild-type viral sequence or a defective genome. Techniques and methods for these analyses are well known in the art and have been previously described. This methodology will enable one skilled in the art to obtain related (wild-type or defective) forms of the viral genome.
Detection of Sequences in Captured Particles Which When Amplified by PCR Hybridize to HCV cDNA Derived from Clone 81
The RNA in captured particles was obtained as described below. The analysis for sequences which hybridize to the HCV cDNA derived from clone 81 was carried out utilizing the PCR amplification procedure, as described supra., except that the hybridization probe was a kinased oligonucleotide derived from the clone 81 cDNA sequence. The results showed that the amplified sequences hybridized with the HCV cDNA probe.
Particles were captured from HCV infected chimpanzee plasma using polystyrene beads coated with an immunopurified antibody directed against the polypeptide encoded in clone 5-1-1. The procedure for producing the immunopurified antibody preparation is described in U.S. Ser. No. 07/456,637, which is commonly owned by the herein assignee, and which is incorporated herein by reference. Briefly, the HCV polypeptide encoded within clone 5-1-1 was expressed as a fusion polypeptide with superoxide dismutase (SOD). This was accomplished by subcloning the clone 5-1-1 cDNA insert into the expression vector pSODcf1 (Steimer et al. (1986)). DNA isolated from pSODcf1 was treated with BamHI and EcoRI, and the following linker was ligated into the linear DNA created by the restriction enzymes:
5' GAT CCT GGA ATT CTG ATA AGA CCT TAA GAC TAT TTT AA 3'
After cloning, the plasmid containing the insert was isolated. Plasmid containing the insert was restricted with EcoRI. The HCV cDNA insert in clone 5-1-1 was excised with EcoRI, and ligated into this EcoRI linearized plasmid DNA. The DNA mixture was used to transform E. coli strain D1210 (Sadler et al. (1980)). Recombinants with the 5-1-1 cDNA in the correct orientation for expression of the ORF were identified by restriction mapping and nucleotide-sequencing. Recombinant bacteria from one clone were induced to express the SOD-NANB5-1-1 polypeptide by growing the bacteria in the presence of IPTG. The fusion polypeptide was purified from the recombinant E. coli by differential extraction of the cell extracts with urea, followed by chromatography on anion and cation exchange columns. The purified SOD-NANB5-1-1 polypeptide was attached to a nitrocellulose membrane. Antibody in samples of HCV infected serum was absorbed to the matrix-bound polypeptide. After washing to remove non-specifically bound materials and unbound materials, the bound antibody was released from the bound polypeptide.
cPCR Method to Detect HCV RNA in Liver and in Serum from Individuals with NANBH
The reliability and utility of a modified form of the PCR assay, i.e., a cPCR assay, for detecting HCV infection was determined by performing the assay on total liver RNA and on serum from infected individuals. In the cPCR assay, putative viral RNA in the sample is reverse transcribed into cDNA with reverse transcriptase; a segment of the resulting cDNA is then amplified utilizing a modified version of the PCR technique described by Saiki et al. (1986). The primers for the cPCR technique are derived from HCV RNA, which can be identified by the family of HCV cDNAs provided herein. Amplified product corresponding to the HCV-RNA is detected utilizing a probe derived from the family of HCV cDNAs provided herein.
The cPCR/HCV assay used in these studies were performed utilizing the following methods for the preparation of RNA, the reverse transcription of the RNA into cDNA, the amplification of specific segments of the cDNA by PCR, and the analysis of the PCR products.
RNA was extracted from liver utilizing the guanidium isothiocyanate method for preparing total RNA described in Maniatis et al. (1982).
In order to isolate total RNA from plasma, the plasma was diluted five- to ten-fold with TENB (0.1M NaCl, 50 mM Tris-HCl, pH 8.0, 1 mM EDTA) and incubated in a Proteinase K/SDS solution (0.5% SDS, 1 mg/ml Proteinase K, 20 micrograms/ml Poly A carrier) for 60 to 90 minutes at 37.degree. C. The samples were extracted once with phenol (pH 6.5), the resulting organic phase was re-extracted once with TENB containing 0.1% SDS, and the aqueous phases of both extractions were pooled and extracted twice with an equal volume of phenol/CHCl.sub.3 /isoamyl alcohol �1:1(99:1)!. The resulting aqueous phases were extracted with an equal volume of ChCl.sub.3 /isoamyl alcohol (99:1) twice, and ethanol precipitated using 0.2M sodium acetate, pH 6.5, and 2.5 volumes of 100% ethanol; precipitation was overnight at -20.degree. C.
The cDNA used as a template for the PCR reaction was prepared utilizing the designated samples for preparation of the corresponding cDNAs. Each RNA sample (containing either 2 micrograms of heat denatured total chimpanzee liver RNA, RNA from 2 microliters of plasma, or 10% of the RNA extracted from 10mm.times.4 mm cylindrical human liver biopsies) was incubated in a 25 microliter reaction containing 1 micromolar of each primer, 1 millimolar of each deoxyribonucleotide triphosphate (dNTP), 50 millimolar Tris-HCL, pH 8.3, 5 millimolar MgCl.sub.2, 5 millimolar dithiothreitol (DTT), 73 millimolar KCl, 40 units of RNase inhibitor (RNASIN), and 5 units of AMV reverse transcriptase. The incubation was for 60 minutes at 37.degree. C. Following cDNA synthesis, the reactions were diluted with 50 microliters of deionized water (DIW), boiled for 10 minutes, and cooled on ice.
Amplification of a segment of the HCV cDNA was performed utilizing two synthetic oligomer 16-mer primers whose sequences were derived from HCV cDNA clones 36 (anti-sense) and 37b (sense). The sequence of the primer from clone 36 was:
5' GCA TGT CAT GAT GTA T 3'.
The sequence of the primer from clone 37b was:
5' ACA ATA CGT GTG TCA C 3'.
The primers were used at a final concentration of 1 micromolar each. In order to amplify the segment of HCV cDNA which is flanked by the primers, the cDNA samples were incubated with 0.1 microgram of RNAse A and the PCR reactants of the Perkin Elmer Cetus PCR kit (N801-0043 or N801-0055) according to the manufacturer's instructions. The PCR reaction was performed for either 30 cycles or 60 cycles in a Perkin Elmer Cetus DNA thermal cycler. Each cycle Consisted of a 1 minute denaturation step at 94.degree. C., an annealing step of 2 minutes at 37.degree. C., and an extension step of 3 minutes at 72.degree. C. However, the extension step in the final cycle (30 or 60) was 7 minutes rather than 3 minutes. After amplification the samples were extracted with an equal volume of phenol: chloroform (1:1), followed by extraction with an equal volume of chloroform, and then the samples were precipitated with ethanol containing 0.2M sodium acetate.
The cPCR products were analyzed as follows. The products were subjected to electrophoresis on 1.8% alkaline agarose gels according to Murakawa et al. (1988), and transferred onto Zeta.TM. Probe paper (BioRad Corp.) by blotting gels overnight in 0.4M NaOH. The blots were neutralized in 2.times.SSC (1.times.SSC contains 0.15M NaCl, 0.015M sodium citrate), prehybridized in 0.3M NaCl, 15 mM sodium phosphage buffer, pH 6.8, 15 mM EDTA, 1.0% SDS, 0.5% nonfat milk (Carnation Co.), and 0.5 mg/ml sonicated denatured salmon sperm DNA. The blots to be analyzed for HCV cDNA fragments were hybridized to a .sup.32 P-labeled probe generated by nick translation of the HCV cDNA insert sequence in clone 35, described in U.S. Ser. No. 07/456,637. After hybridization, the blots were washed in 0.1.times.SSC (1.times.SSC contains 0.15M NaCl, 0.01M Na citrate) at 65.degree. C. dried, and autoradiographed. The expected product size is 586 nucleotides in length; products which hybridized with the probe and migrated in the gels in this size range were scored as positive for viral RNA.
As a control, cPCR primers designed to amplify alpha-1 anti-trypsin mRNA was performed to verify the presence of RNA in each sample analyzed. The coding region of the alpha-1 anti-trypsin gene is described in Rosenberg et al. (1984). Synthetic oligomer 16-mer primers designed to amplify a 365 nucleotide fragment of the coding region of the alpha-1 antitrypsin gene were derived from nucleotides 22-37 (sense) and nucleotides 372-387 (antisense). The PCR products were detected using a .sup.32 P nick-translated probe which lies between, and not including, the cDNA/PCR primer sequences.
Due to the extreme sensitivity of the PCR reaction, all samples were run a minimum of three times. All false positive signals were eliminated when the following precautions were taken: 1) eliminating aerosols by using screw capped tubes with rubber O-ring seals; 2) pipetting with Ranin Microman.TM. positive displacement pipetters with disposable pistons/capillaries; and 3) selecting the oligonucleotide sequences for the cDNA and PCR primers from two non-contiguous cDNA clones.
Detection of HCV RNA in Liver Samples by a cPCR Method
The cPCR assay was performed on total RNA isolated from livers of three chimpanzees experimentally infected with a NANBH agent, and from liver biopsies of Italian patients diagnosed as having chronic NANBH.
FIG. 8A shows the results of the cPCR assay using 1 microgram of each preparation of total liver RNA. The RNA was isolated from liver samples of a chimpanzee in the chronic phase of NANBH (910)(lane 1), two chimpanzees in the acute phase of infection (1028 and 508)(lanes 2 and 3, respectively). PCR was performed on the samples in lanes 1-3 for 30 cycles and the autoradiogram of the blot containing those lanes was exposed for 5 hours. cDNA from 1 microgram of total RNA from acutely infected animal 1028 (lane 4), and three uninfected chimpanzees (lanes 5-7), were amplified for 60 cycles and the autoradiograms containing those lanes were exposed for 7 days. .sup.32 P labeled MspI-digested pBR322 DNA served as markers on all the autoradiograms. It may be seen from the results that cDNA corresponding to HCV RNA was seen only in the samples from Chimpanzees with NANBH, whether acute or chronic (lanes 1, 3, and 4). The cPCR products in these lanes migrated between marker fragments of 527 and 622 nucleotides (not shown).
FIG. 8B shows the results of the cPCR assay using 10% of the RNA extracted from 10 mm.times.4 mm liver biopsy cylinders from 15 chronic NANB patients (lanes 1-15), one patient with cryptogenic liver disease (lane 16) and one control sample from a patient with chronic Hepatitis B (lane 17). Amplification by PCR was for 30 cycles and the autoradiogram for the blots were exposed for 4 days, except that lane 1 was exposed for 15 hours. As seen from the results, 9/15 (60%) of the human samples were positive for HCV RNA (lanes 1, 2, 4, 6, 7, 10-13). One patient diagnosed with cryptogenic liver disease (lane 16) and one patient with a Chronic HBV infection (lane 17) were repeatedly negative in the cPCR assay.
Comparison of the HCV/cPCR Assay on Human Liver Biopsies and RIA of Serum Using HCV C100-3 Polypeptide
SOD/HCV C100-3 polypeptide (also called C100) is a recombinant fusion polypeptide which contains 363 viral amino acids. The polypeptide is useful for detecting antibodies to HCV (See Kuo et al. (1989)).
Radioimmune assay using C100 was performed on the sera collected from the same 17 human patients whose liver samples were subjected to HCV/cPCR assay as described in supra. The sera was collected on the same day as the liver biopsies. The assay was performed essentially as described in U.S. Ser. No. 07/456,637, which is commonly owned and incorporated herein by reference. Briefly, Microtiter plates (Immulon 2, Removeawell strips) were coated with 0.1 microgram of purified C100. The coated plates were incubated for 1 hour at 37.degree. C. with the serum samples (100 microliters of a 1:100 dilution) or appropriate controls. After incubation, the unbound material was removed, the plates were washed, and complexes of human antibody-C100 were detected by incubation with .sup.125 I-labeled sheep anti-human immunoglobulin. Unbound labeled antibody was removed by aspiration, and the plates were washed. The radioactivity in individual wells was determined.
The results of the RIA showed that sixty-seven percent of these samples were positive for anti-C100 antibodies. Sera from the patient diagnosed with cryptogenic liver disease was positive for anti-C100 antibodies, although the levels of, viral RNA were undetectable in the patient's liver in this sample. The level of correlation between the presence of anti-C100 antibodies and HCV RNA was seventy percent; two patients who were negative for antibodies by RIA had significant levels of HCV RNA in their livers (data not shown).
The results indicate that virus is frequently present in the liver of patients with circulating anti-C-100 antibodies, and confirms claims that the presence of anti-C100 antibodies accurately reflects exposure to HCV. Moreover, taken together, these results indicate that HCV of this type accounts for NANBH in at least 75% of the patients in this study, and that the predominant strain of HCV in Italy appears to be closely related to the strain of HCV prevalent in the United States.
HCV/cPCR Assay of Sera: Detection of Viral RNA in Acute Phase Infection in Chimpanzees
The temporal relationship between the display of liver damage, the presence of HCV RNA, and the presence of anti-HCV antibodies was monitored in serum from two experimentally infected chimpanzees with NANBH (nos. 771 and 910). Liver damage was determined by alanine amino transferase (ALT) levels; the presence of HCV RNA was determined by the HCV cPCR assay described above; anti-HCV antibodies were detected utilizing the C100 RIA.
The HCV/cPCR analysis was performed on RNA extracted from 1 microliter of chimpanzee plasma. Serum was taken from chimpanzee 771 on days 25, 32, 70 and 88 post-infection; cPCR was performed for 30 cycles and the autoradiogram was exposed for 18 days. Serum was taken from chimpanzee 910 on days 11, 28, and 67 post-infection; cPCR was performed for 60 cycles and the autoradiogram was exposed for 5 days.
The results of the assays are shown in FIG. 9A for chimpanzee 771, and FIG. 9B for chimpanzee 910. From a comparison of FIGS. 9A and 9B, it appears that an early, well defined peak of ALT values during acute hepatitis correlates with the presence of viral RNA in the infected individual.
The data also indicate that the presence of HCV RNA, which is indicative of a state of viremia, precedes the presence of anti-HCV antibodies. Chimpanzee 771 (FIG. 9A) exhibited a clearly defined acute episode of post-transfusion NANBH at 28 days, as characterized by an initial peak of ALT levels. HCV RNA was detected in the serum collected at day 25, and at day 32. However, during this acute phase, anti-HCV antibodies were absent. In contrast, at day 70 HCV RNA was below the experimental level of detection, and anti-HCV antibodies were rising. At day 88, HCV RNA remained undetectable, while anti-HCV antibodies were significantly increased over that of day 70.
The results obtained from the sera of chimpanzee 910 were somewhat similar in pattern, although the time of HCV antibodies induced by the infection were not detected during the acute phase of the disease, Which extended to at least day 67; the anti-HCV antibodies detected by RIA at day 11 were due to passive immunization of animal 910 with antibodies from the plasma used to inoculate the animal. Anti-HCV antibodies were found in chimpanzee 910 serum during the later, chronic phase of the infection (data not shown).
It should be noted that low ALT values in plasma from individuals with chronic NANBH do not necessarily correlate with weak virus production. A pool of 17 different plasma samples taken from chimpanzee 910 over a period of two to three and one-half years post inoculation was monitored for ALT levels and for HCV RNA. The ALT values of the samples did not exceed 45 mU/ml; nevertheless, titration studies indicated high titers of HCV (3.times.10.sup.6 CID/ml). cPCR was carried out for 30 cycles, and the autoradiogram was exposed for 15 hours; the cPCR analysis clearly showed the presence of viral RNA (data not shown).
HCV/cPCR Assay of Sera: Detection of Viral RNA in Acute Phase Infection in Humans
Plasma from a human surgical patient collected during early acute NANBH was examined for HCV RNA and for anti-HCV antibodies, utilizing the HCV/cPCR assay and C100-RIA, respectively. The HCV/cPCR assay was conducted utilizing 1 microliter of plasma from the patient, and from four human controls with known pedigrees; cPCR was performed for thirty cycles, and after hybridization and washing the autoradiogram was exposed for eight hours.
The results showed that the serum collected from the surgical patient during the acute phase of infection contained a high level of viral RNA, and that anti-HCV antibodies were not detectable by the C100-RIA (data now shown). (The acute phase plasma from the surgical patient was known to have a high titer of NANBH infectious agent �10.sup.6.5 CID/ml, as determined by Feinstone et al. (1981); Feinstone et al. (1983)!). It should be noted, however, that this patient did sero-covert to anti-HCV antibodies by the C100-RIA approximately 9 months after infection. The serum from the pedigreed human control plasmas were negative in both the HCV/cPCR assay and C100-RIA.
Sensitivity of HCV/cPCR Assay
The sensitivity of the HCV/cPCR assay was determined by analyzing ten-fold serial dilutions of a plasma pool of known titer. The chimpanzee plasma had a titer of .about.3.times.10.sup.5 CID/mi, and RNA was extracted from ten-fold dilutions of 1 microliter of the plasma. cPCR was performed for 30 cycles, and after hybridization and washing, the autoradiogram Was exposed for 15 hours. The cPCR products resulting from amplification of .about.300, .about.30, and .about.3 CID of HCV genomes are shown in lanes 1-3, respectively of FIG. 12. The samples in lanes 1 and 2 were detectable on autoradiograms exposed for 2 hours.
Since the average titer of HCV in infected individuals is believed to be between approximately 100 to 10,000 CID/ml of plasma, this data suggests that the HCV/cPCR assay may be clinically useful.
HCV/cPCR Assay for Variant HCV Strains
Primers, consisting of a set of oligomer 44-mers and a set of oligomer 45-mers, were designed to amplify strains of HCV which are similar or identical to the HCV isolate from which the cDNA sequence in FIG. 10 is derived. The premise underlying the design of these primers is our discovery that HCV is a Flavi-like virus. Members of the Flaviviridae family, when compared to HCV, have two major conserved sets of amino acid sequences, TATPPG and QRRGR, in the putative NS3 region of these viruses. Several other smaller sets may be seen, for example, GDD in the putative NS5 region. Other sets are determinable by comparison of the known amino acid sequences with that of HCV. This information was deduced from the sequences for several members of Flaviviridae which have been described, including Japanese Encephalitis Virus (Sumiyoshi et al. (1987)), Yellow Fever Virus (Rice et al. (1985)), Dengue Type 2 Virus (Hahn et al. (1988)), Dengue Type 4 Virus (Mackow (1987)), and West Nile Virus (Castle et al. (1986)). The conserved amino acid sequences and codon utilization are in the table immediately following.
__________________________________________________________________________Conserved Amino Acid (A.A.) Sequences among Flavivirues and__________________________________________________________________________HCVVirus # of 1st A.A. A.A. = T A T P P G__________________________________________________________________________HCV 1348 5' ACC GCC ACC CCT CCG GGC 3'Yellow Fever 1805 ACA GCC ACA CCG CCT GGGWest Nile 1818 ACG GCA ACG CCA CCC GGGDengue-4 1788 ACC GCA ACC CCT CCC GGAJEV 1957 ACA GCG ACC CCG CCT GGAHCV sense primer (44mer) = 5' . . . ACC GCC ACC CCX CC 3' (X = A.T.C.G)__________________________________________________________________________Virus # of 1st A.A. A.A. = Q R R G R__________________________________________________________________________HCV 1486 5' CAA CGT CGG GGC AGG 3'Yellow Fever 1946 CAA AGG AGG CGG CGCWest Nile 1959 CAG CGG AGA GGA CGCDengue-4 1929 CAG AGA AGA GGG CGAJEV 1820 CAA CGG AGG GGC AGAHCV antisense primer (45mer) = 3' GTX GCA GCC CCG TCC . . . 5' (X = T.C)__________________________________________________________________________ Note: the primer sequence was chosen to minimize the number of nucleotide degeneracies at the 3' end of the primer sequence and maximize of the number of nucleotides at the 3' end of each primer which exactly match an of the possible nucleotide sequences, or the complement thereof, encoding the conserved amino acids indicated above.
The 44-mer and 45-mer oligomer primers were designed so that the sequences encoding these amino acids were incorporated within the primer. Moreover, they contain degeneracies at the 3'-end of each primer, and are derived from two different regions of the HCV genome which are present in clone 40b (See FIG. 11), and which are derived from the region encoding putative NS3 of HCV. The formulae for the oligonucleotide primers in the sets are:
5' GAC TGC GGG GGC GAG ACT GGT TGT GCT CGC ACC GCC ACC CCX CC 3'
where X is A, T, G, or C; and
5' TCT GTA GAT GCC TGG CTT CCC CCT GCC AGT CCT GCC CCG ACT YTG 3'
where Y is T or C.
The HCV/cPCR assay was carried out utilizing these primers to amplify HCV RNA in chimpanzee 910 plasma. The assay method was essentially as described in Section supra., except that the 44-mer and 45-mer sets of oligomer primers were substituted for the primers derived from clone 36 and clone 37b. In addition, detection of amplified HCV cDNA was by hybridization with-a probe derived from clone 40a, the sequence of which is shown in FIG. 13.
The probe was prepared by amplifying the segment of clone 40a indicated in the figure utilizing the PCR method described supra., and 18-mer primers containing the following sequences:
5' GAG ACA TCT CAT CTT CTG 3'
and
5' GAG CGT GAT TGT CTC AAT 3'.
After amplification, the probe preparation was labeled with .sup.32 P by nick translation.
FIG. 14 shows an autoradiograph of the Southern blots probed with the sequence derived from Clone 40a. .sup.32 P labeled MspI digested pBR322 DNA fragments served as markers (lane 1). The predicted size of the PCR product resulting from amplification using these primers is 490 nucleotides (nt). Duplicate reactions are shown in lanes 2 and 3.
Analysis for Variants of the 5'-Region of HCV
Based upon the Flavivirus model, the 5'-region HCV cDNA which is flanked by the regions represented in clones ag30a and k9-1 encodes a segment of putative envelope and/or matrix protein(s) (E/M). Serum obtained from the chimpanzee from which the HCV cDNA "c" library, was constructed was analyzed by HCV/cPCR to determine whether variants within this target region were present.
The HCV/cPCR assay was performed essentially as described supra., except for the primers and probes used. FIG. 15 shows the relationship of the primers and probes, and clones from which they were derived, to that of the target region of HCV cDNA. One set of PCR primers, ag30a16A and K91Env16B, were derived from clones ag30a and k9-1, which are upstream and downstream, respectively, of the target sequence. The expected size of the cPCR product primed by ag30a16A and K91Env16B is 1.145 kb based upon the confirmed sequence of HCV cDNA. Two other sets of PCR primers covering the region amplified using ag30a16A and K91Env16B, and overlapping each other were also used for PCR amplification of HCV RNA in the serum. Thus, in this case the PCR reactions were run using as one set of primers ag30a16A and CA156e16B, and as the second set of primers CA156e16A and k91Env16B. The expected PCR product sizes for these pairs were 615 nucleotides (NT) and 683 NT, respectively. The table immediately following lists the primer, the clone from which it was derived, and the primer sequence.
TABLE______________________________________Primer Clone Sequence______________________________________ag30a16A ag30a 5' CTC TAT GGC AAT GAG G 3'K91Env16B k9-1 5' CGT TGG CAT AAC TGA T 3'CA156e16B 156 5' CGA CAA GAA AGA CAG A 3'CA156e16A 156 5' AGC TTC GAC GTC ACA T 3'CA216a16A 216 5' TGA ACT ATG CAA CAG G 3'CA216a16B 216 5' GGA GTG TGC AGG ATG G 3'CA84a16A 84 5' AAG GTT GCA ATT GCT C 3'CA84a16B 84 5' ACT AAC AGG ACC TTC G 3'______________________________________
The probes for all of the HCV/cPCR products consisted of .sup.32 P labeled sections of HCV cDNA which had been prepared by PCR amplification of a region of clone 216 (using CA216a16A and 216a16B as primers), and of clone 84 (using CA84a16A and CA84a16B as primers); .sup.32 P was introduced into the PCR products by nick translation. These probes did not overlap the primers used in the HCV/cPCR reactions.
FIG. 16 shows an autoradiograph of a Southern blot in which the HCV/cPCR products were hybridized with the .sup.32 P-labeled probes. The HCV/cPCR product extended from primers ag30a16A and K91Env16B (lane 1) was approximately 1.1 Kb; no other PCR products were observed in a 15 hour exposure. The HCV products extended from the primer sets ag30a15A/CA156e16B (lane 2) and CA156e16A/K91Env16B (lane 3) were approximately 625NT and approximately 700 NT, respectively. The size of the PCR products were determined by comparison with the relative migrations of fragments resulting from the digestion of pBR322 with MspI and of PhiX 174 digested with HaeIII (lane 5).
The above study will detect insertions or deletions as small as approximately 20NT to 50NT and DNA rearrangements altering the size of the target DNA. The results in FIG. 16 confirm that there is only 1 major species of cDNA derived from the E/M region of the HCV in the chimpanzee serum.
Amplification for Cloning of HCV cDNA Sequences Utilizing the PCR and Primers Derived from Conserved Regions of Flavivirus Genomic Sequences
Our discovery that HCV is a flavi-like virus, allows a strategy for cloning uncharacterized HCV cDNA sequences utilizing the PCR technique, and primers derived from the regions encoding conserved amino acid sequences in flaviviruses. Generally, one of the primers is derived from a defined HCV genomic sequence, and the other primer which flanks a region of unsequenced HCV polynucleotide is derived from a conserved region of the flavivirus genome. The flavivirus genomes are known to contain conserved sequences within the NS1, and E polypeptides, which are encoded in the 5'-region of the flavivirus genome. Thus, to isolate cDNA sequences derived from putatively comparable regions of the HCV genome, upstream primers are designed which are derived from the conserved sequences within these flavivirus polypeptides. The downstream primers are derived from an upstream end of the known portion of the HCV cDNA.
Because of the degeneracy of the code, it is probable that there will be mismatches between the flavivirus probes and the corresponding HCV genomic sequence. Therefore a strategy which is similar to the one described by Lee (1988) is used. The Lee procedure utilizes mixed oligonucleotide primers complementary to the reverse translation products of an amino acid sequence; the sequences in the mixed primers takes into account every codon degeneracy for the conserved amino acid sequence.
Three sets of primer mixes are generated, based on the amino acid homologies found in several flaviviruses, including Dengue-2,4 (D-2,4), Japanese Encephalitis Virus (JEV), Yellow Fever (YF), and West Nile Virus (WN). The primer mixture derived from the most upstream conserved sequence (5'-1), is based upon the amino acid sequence gly-trp-gly, which is part of the conserved sequence asp-arg-gly-trp-gly-aspN found in the E protein of D-2, JEV, YF, and WN. The next primer mixture (5'-2) is based upon a downstream conserved sequence in E protein, phe-asp-gly-asp-ser-tyr-ileu-phe-gly-asp-ser-tyr-ileu, and is derived from phe-gly-asp; the conserved sequence is present in D-2, JEV, YF, and WN. The third primer mixture (5'-3), is based on the amino acid sequence arg-ser-cys, which is part of the conserved sequence cys-cys-arg-ser-cys in the NS1 protein of D-2, D-4, JEV, YF, and WN. The individual primers which form the mixture in 5'-3 are shown in FIG. 13. In addition to the varied sequences derived from conserved region, each primer in each mixture also contains a constant region at the 5'-end which contains a sequence encoding sites for restriction enzymes, HindIII, MboI, and EcoRI.
The downstream primer, ssc5h20A, is derived from a nucleotide sequence in clone 5h, which contains HCV cDNA with sequences with overlap those in clones 14i and 11b. The sequence of ssc5h20A is
5' GTA ATA TGG TGA CAG AGT CA 3'.
An alternative primer, ssc5h34A, may also be used. This primer is derived from a sequence in clone 5h, and in addition contains nucleotides at the 5'-end which create a restriction enzyme site, thus facilitating cloning. The sequence of ssc5h34A is
5' GAT CTC TAG AGA AAT CAA TAT GGT GAC AGA GTC A 3'.
The PCR reaction, which was initially described by Saiki et al. (1986), is carried out essentially as described in Lee et al. (1988), except that the template for the cDNA is RNA isolated from HCV infected chimpanzee liver, or from viral particles isolated from HCV infected chimpanzee serum. In addition, the annealing conditions are less stringent in the first round of amplification (0.6M NaCl, and 25.degree. C.), since the part of the primer which will anneal to the HCV sequence is only 9 nucleotides, and there could be mismatches. Moreover, if ssc5h34A is used, the additional sequences not derived from the HCV genome tend to destabilize the primer-template hybrid. After the first round of amplification, the annealing conditions can be more stringent (0.066M NaCl, and 32.degree. C.-37.degree. C.), since the amplified sequences now contain regions which are complementary to, or duplicates of the primers. In addition, the first 10 cycles of amplification are run with Klenow enzyme I, under appropriate PCR conditions for that enzyme. After the completion of these cycles, the samples are extracted, and run with Taq polymerase, according to kit directions, as furnished by Cetus/Perkin-Elmer.
After the amplification, the amplified HCV cDNA sequences are detected by hybridization using a probe derived from clone 5h. This probe is derived from sequences upstream of those used to derive the primer, and does not overlap the sequences of the clone 5h derived primers. The sequence of the probe is
5' CCC AGC GGC GTA CGC GCT GGA CAC GGA GGT GGC CGC GTC GTG TGG CGG TGT TGT TCT CGT CGG GTT GAT GGC GC 3'.
Detection of HCV RNA in Plasma Using HCV/cPCR, Using Primers and Probes Derived from the 5'-Region of HCV RNA
Extraction of HCV RNA from Plasma
Frozen plasma was thawed in a P3 hood. A 0.2 ml aliquot of digestion mix (100 millimolar Tris-HCl, 2 millimolar. EDTA, 200 millimolar NaCl, 20 microgram/milliliter MS2 RNA, 0.5% SDS, and 2 milligram/milliliter Proteinase K, pH 8) was added to a 2 milliliter microfuge tube and prewarmed to 37.degree. C. The plasma (0.2 milliliter) was then added, and the mixture allowed to incubate at 60.degree. C. for 1 hour. Next, phenol (0.4 milliliter) was added, and the mixture vortexed 3.times. for 30 seconds, allowing 30 seconds between each vortexing. The mixture was then centrifuged for 5 minutes, and the aqueous phase recovered. The organic phase was back-extracted with 0.1 milliliter TES, and the pooled aqueous phases extracted 2.times. with 1 volume phenol/chloroform/IAA, then with 1 volume chloroform. To the extract was added 1 microliter (2 micrograms) glycogen (Boehringer Mannheim Corp.) and 25 microliter NaOAc (3 molar, pH 5.4). and 1.25 ml cold EtOH, and the product frozen on dry ice and spun 10 min. The pellet was dissolved in 100 microliter distilled water, and 5 microliters NaOAc (pH 5.4) added with 250 microliters cold EtOH. The solution was frozen and spun again, and the resulting pellet dissolved in 10 microliters dH.sub.2 O.
cDNA Synthesis
In order to synthesize cDNA from the extracted RNA, initially 5 microliters of the dissolved RNA was incubated with 4 microliters water and 1 microliter (1 microgram or 166 pmols of 18-mer) of RT primer. The incubation was at 70.degree. C. for 3 minutes, followed by chilling on ice. The sequence of the RT primer was:
5' CCC AAC ACT ACT CGG CTA 3'.
After the initial incubation, the following were added to the incubation mixture: 10 microliters of 5.times. first strand buffer (250 mM Tris HCl, pH 8.3, 375 mM KCl, 15 mM MgCl.sub.2, 50 mM dithiothreitol); 2.5 microliters deoxynucleoside triphosphates (10 mM each); 2.5 microliters reverse transcriptase (MMLV from BRL, 200 units per microliter), and distilled water to bring the volume to 50 microliters. The mixture was incubated at 37.degree. C. for 1 hour, heated at 90.degree. C. for 3 minutes, and chilled on ice.
PCR Amplification
PCR amplification of the HCV cDNA produced above was conducted using the control and test reagents listed below, in the Table. In the Table, "RNA" indicates a control sample in which the RNA was extracted from an individual which was uninfected with HCV1; this sample was carried through the steps of cDNA synthesis and PCR amplification. "cDNA" indicates a control sample which was carried through the steps of cDNA synthesis and PCR amplification; however, in this case the aliquot of RNA was replaced with water during cDNA synthesis. "Template.sup.- " was a control for the PCR reaction from which the template was omitted. "Sample" indicates the serum which was tested for the presence of HCV RNA.
TABLE______________________________________ RNA CDNA Template.sup.- Sample (ul) (ul) (ul) (ul)______________________________________water 100.0 to 100.0 to 100.0 to 100.010x buffer 10.0 10.0 10.0 10.0dNTPs 16.0 16.0 16.0 16.0primer 1 0.5 0.5 0.5 0.5primer 2 0.5 0.5 0.5 0.5template 2.5 2.5 -- 2.5Taq Pol 0.3 0.5 0.5 0.5 0.5______________________________________
Primer 1 and Primer 2 were 0.5 micrograms/microliter and 0.42 micrograms/microliter, respectively, and had the following sequences.
Primer 1: 5' ACC ATG AAT CAC TCC CCT GTG AGG AAC TAC 3'
Primer 2: 5' AGT CTT GCG GGG GCA CGC CCA AAT C 3'
These primers hybridize to a conserved region in the 5' end of the HCV genome. The samples contained 12.5 microliter serum equivalents of HCV cDNA. The amplification cycle conditions used were as follows:
35 cycles: Melting--94.degree. C. for 1.5 min
Annealing--60.degree. C. for 2 min
Elongation--72.degree. C. for 3 min
Final Elongation: 72.degree. C. for 30 min
Soak at 4.degree. C. until removed.
The amplified product was probed using a labeled DNA. The sequence of the probe was the following.
Probe: 5' TTT CTT GGA TCA ACC CGC TCA ATG CCT GGA 3'
The detection of amplified DNA which hybridized with the probe was as described in the Examples described supra.
Over 200 HCV sera-positive samples were examined by the above procedure. Only results in which the controls were negative were considered, In this case, all sera-positive samples were also positive in the PCR assay.
Probes Derived from the Putative "Core" Region of HCV RNA
An analysis of the nucleotide sequences of cDNAs to different HCV isolates shows that a high degree of sequence conservation exists in the region from about nucleotide 1 to about nucleotide 570, using the numbering system for nucleotides shown in FIG. 1. This region putatively encodes a "core" polypeptide of HCV. The consensus sequences for five different isolates from different geographic locations (Japan and the U.S.) is shown in FIG. 18, where HCV1 is the prototype HCV; the amino acids encoded in the large ORF of HCV1 are shown above the consensus nucleotide sequences. In the sequences shown in FIG. 18, HCV-JH is from a personal communication from Dr. Tetsu Miyamura (National Institute of Health of Japan), and JC-J1 and JC-J4 are from Mayumi et al. (1990). It may be seen in FIG. 18 that between the consensus sequences in the putative "core" region, there is at least 90% homology relative to the sequence in HCV1. In view of the high degree of homology in the region between nucleotides +1 to +571, probes to this area would be useful in screening for HCV positive Biological specimens.
A set of label probes which may be used for the detection of HCV RNA from the region which putatively encodes an HCV "core" polypeptide are shown in FIG. 19. In FIG. 19, a "probe number" in the set includes a series of polynucleotides with heterogeneities indicated by the by the IUB Group Code listed in FIG. 19. The heterogeneities are to accomodate nucleotide sequence differences found in the consensus sequences of the different isolates. The regions of the HCV sequence to which the probes in the probe set of FIG. 19 are complementary are shown in FIG. 20, in which the nucleotide numbers correspond to the numbering in FIG. 1.
Industrial Applicability
The methods described herein, as well as the oligomers, both probes and primers, derived from HCV cDNA, and kits containing them, are useful for the accurate, relatively simple, and economic determination of the presence of HCV in biological samples, more particularly in blood which may be used for transfusions, and in individuals suspected of having HCV an infection. Moreover, these methods and oligomers may be useful for detecting an earlier stage of HCV infection than are immunological assays based upon the use of a recombinant HCV polypeptides. Also, an amplified polynucleotide hybridization assay detects HCV RNA in occasional samples which are anti-HCV antibody negative. Thus, the probes and primers described herein may be used amplified hybridization assays, in conjunction with an immunoassays based on HCV polypeptides to more completely identify infections due to HCV, and HCV-infected biological specimens, including blood.
The information provided herein allows the design of primers and/or probes which are derived from conserved regions of the HCV genome. The provision of these primers and probes makes available a general method which will detect variant HCV strains, and which will be of use in the screening of blood and blood products.
If the primers used in the method are derived from conserved regions of the HCV genome, the method should aid in the detection and/or identification of variant strains of HCV. This, in turn, should lead to the development of additional immunological reagents for the detection and diagnosis of HCV, as well as the development of additional polynucleotide reagents for detection and or treatment of HCV.
In addition, sets of primers and probes designed from the conserved amino acid sequences of Flaviviruses and HCV allow for a universal detection method for these infectious agents.
The following listed materials are on deposit under the terms of the Budapest Treaty with the American Type Culture Collection (ATCC), 12301 Parklawn Dr., Rockville, Md. 20852, and have been assigned the following Accession Numbers.
______________________________________lambda-gt11 ATCC No. Deposit Date______________________________________HCV cDNA library 40394 1 Dec. 1987clone 81 40388 17 Nov. 1987clone 91 40389 17 Nov. 1987clone 1-2 40390 17 Nov. 1987clone 5-1-1 40391 18 Nov. 1987clone 12f 40514 10 Nov. 1988clone 35f 40511 10 Nov. 1988clone 15e 40513 10 Nov. 1988clone K9-1 40512 10 Nov. 1988JSC 308 20879 5 May 1988pS356 67683 29 April 1988______________________________________
In addition, the following deposits were made on 11 May
______________________________________Strain Linkers ATCC No.______________________________________D1210 (Cf1/5-1-1) EF 67967D1210 (Cf1/81) EF 67968D1210 (Cf1/CA74a) EF 67969D1210 (Cf1/35f) AB 67970D1210 (Cf1/279a) EF 67971D1210 (Cf1/C36) CD 67972D1210 (Cf1/13i) AB 67973D1210 (Cf1/C33b) EF 67974D1210 (Cf1/CA290a) AB 67975HB101 (AB24/C100 #3R) 67976______________________________________
______________________________________Strain Derivative ATCC No.______________________________________pCF1CS/C8f 67956pCF1AB/C12f 67952pCF1EF/14c 67949pCF1EF/15e 67954pCF1AB/C25c 67958pCF1EF/C33c 67953pCF1EF/C33f 67050pCF1CD/33g 67951pCF1CD/C39c 67955pCF1EF/C40b 67957pCF1EF/CA167b 67959______________________________________
______________________________________Strain ATCC No.______________________________________Lambda gt11 (C35) 40603Lambda gt10 (beta-5a) 40602D1210 (C40b) 67980D1210 (M16) 67981______________________________________
The following biological materials were deposited on Mar. 23, 1990.
______________________________________Material ATCC No.______________________________________5'-clone32 (in pUC18S) 68276______________________________________
Upon allowance and issuance of this application as a United States Patent, all restriction on availability of these deposits will be irrevocably removed; and access to the designated deposits will be available during pendency of the above-named application to one determined by the Commissioner to be entitled thereto under. 37 CFR 1.14 and 35 USC 1.22. Moreover, the designated deposits will be maintained for a period of thirty (30) years from the date of deposit, or for five (5) years after the last request for the deposit; or for the enforceable life of the U.S. patent, whichever is longer. The deposited materials mentioned herein are intended for convenience only, and are not required to practice the present invention in view of the descriptions herein, and in addition these materials are incorporated herein by reference.

Claims

1. A method for detecting an HCV sequence in a test sample suspected of containing an HCV polynucleotide, wherein the HCV polynucleotide comprises a selected target region, said method comprising:
(a) providing an oligonucleotide capable of selectively hybridizing to the genome of a hepatitis C virus (HCV), or its complement, wherein the oligonucleotide comprises a contiguous sequence of at least 8 nucleotides fully complementary to the genome of an HCV or its complement;
(b) providing a set of primer oligonucleotides which are printers for the polymerase chain reaction method and which flank the target region;
(c) amplifying the target region via a polymerase chain reaction method to obtain an amplified test sample;
(d) incubating the amplified test sample with the oligonucleotide of step (a) under conditions which allow hybrid duplexes to form between the oligonucleotide and the target region specifically relative to other viral agents; and
(e) detecting any hybrids formed between the target region and the oligonucleotide, wherein the presence of said hybrid duplex is indicative of HCV being present in the test sample.
2. The method of claim 1 wherein the oligonucleotide of step (a) comprises a contiguous sequence of at least 10 nucleotides fully complementary to the genome of an HCV or its complement.
3. The method of claim 1 wherein the oligonucleotide of step (a) comprises a contiguous sequence of at least 12 nucleotides fully complementary to the genome of an HCV or its complement.
4. The method of claim 1 wherein the oligonucleotide of step (a) comprises a contiguous sequence of at least 15 nucleotides fully complementary to the genome of an HCV or its complement.
5. The method of claim 1 wherein the oligonucleotide of step (a) comprises a contiguous sequence of at least 20 nucleotides fully complementary to the genome of an HCV or its complement.
6. The method of claim 1 wherein the oligonucleotide of step (a) comprises a contiguous sequence of at least 10 nucleotides fully complementary to a nucleotide residue sequence present in one of the strands of FIG. 1.
7. The method of claim 1 wherein the oligonucleotide of step (a) comprises a contiguous sequence of at least 10 nucleotides fully complementary to a unique nucleotide residue sequence of the genome of an HCV or its compliment.
8. The method of claim 1 wherein the oligonucleotide of step (a) comprises a contiguous sequence of at least 12 nucleotides fully complementary to a unique nucleotide residue sequence of the genome of an HCV or its complement.
9. The method of claim 1 wherein the oligonucleotide of step (a) comprises a contiguous sequence of at least 15 nucleotides fully complementary to a unique nucleotide residue sequence of the genome of an HCV or its complement.
10. The method of claim 1 wherein the oligonucleotide of step (a) comprises a contiguous sequence of at least 20 nucleotides fully complementary to a unique nucleotide residue sequence of the genome of an HCV or its complement.
11. The method of claim 1 wherein the oligonucleotide of step (a) comprises a contiguous sequence of at least 10 nucleotides fully complementary to a unique nucleotide residue sequence present in one of the strands of FIG. 1.
12. The method of claim 1 wherein the contiguous sequence is a conserved HCV nucleotide sequence.
13. The method of claim 1 wherein the oligonucleotide provided in step (a) comprises a sequence selected from the group consisting of:
5'-TCC CTT GCT CGA TGT ACG GTA AGT GCT GAG AGC ACT CTT CCA TCT CAT CGA ACT CTC GGT AGA GGA CTT CCC TGT CAG GT-3',
5'-CTG TCA GGT ATG ATT CCC GGC TTC CCG GAC-3',
5'-TTT GGC TAG TGG TTA GTG GGC TGG TGA CAG-3',
5'-AAG CCA CCG TGT GCG CTA GGG CTC AAG CCC-3',
5'-CAG GAT GCT GTC TCC CGC ACT CAA CGT-3',
5'-AGT GCA GTG GAT GAA CCG GCT GAT AGC CTT-3',
5'-TCC TGA GGC GAC TGC ACC AGT GGA TAA GCT-3',
5'-CAG GAT GCT GTC TCC CGC ACT CAA CGT C-3',
5'-ATC AGG ACC GGG GTG AGA ACA ATT ACC ACT-3',
5'-AGA GAC AAC CAT GAG GTC CCC GGT GTT C-3',
5'-TCG GAC CTT TAC CTG GTC ACG AGG CAC-3',
5'-ACC TTC CCC ATT AAT GCC TAC ACC ACG GGC-3',
5'-TCC ATC TCT CAA GGC AAC TTG CAC CGC TAA-3',
5'-TCC ATG GCT GTC CGC TTC CAC CTC CAA AGT-3',
5'-GCG ACA ATA CGA CAA CAT CCT CTG AGC CCG-3',
5'-AGC AGA CAA GGG GCC TCC TAG GGT GCA TAA T-3',
5'-CAC CTA TGT TTA TAA CCA TCT CAC TCC TCT-3',
5'-CTC TGT CAC CAT ATT ACA AGC GCT ATA TCA-3',
5'-CTC GTT GCT ACG TCA CCA CAA TTT GGT GTA-3',
5'-TGC TTG TGG ATG ATG CTA CTC ATA TCC CAA-3',
' -AGC AGC GGC GTC AAA AGT GAA GGC TAA CTT-3',
5'-TTC TCG TAT GAT ACC CGC TGC TTT GAC TCC-3',
5'-TGT GTG GCG ACG ACT TAG TCG TTA TCT GTG-3',
5'-CAC ACT CCA GTC AAT TCC TGG CTA GGC AAC-3',
5'-CTG GCT TGA AGA ATC-3',
5'-AGT TAG GCT GGT GAT TAT GC-3',
5'-GAA CGT TGC GAT CTG GAA GAC AGG GAC AGG-3',
5'-TAT CAG TTA TGC CAA CGG AAG CGG CCC CGA-3',
5'-CTG GTT AGC AGO GCT TTT CTA TCA CCA CAA-3',
5'-AAG GTC CTG GTA GTG CTG CTG CTA TTT GCC-3',
5'-ACT GGA CGA CGC AAG GTT GCA ATT GCT CTA-3',
5'-TTC GAC GTC ACA TCG ATC TGC TTG TCG GGA-3',
5'-GGT GAC GTG GGT TTC-3',
5'-GGC TTT ACC ACG TCA CCA ATG ATT GCC CTA-3',
5'-TTT GGG TAA GGT CAT CGA TAC CCT TAC GTG-3',
5'-GAA GCC GCA CGT AAG-3',
5'-CCG GCG TAG GTC GCG CAA TTT GGG TAA-3',
5'-TCA GAT CGT TGG TGG AGT TTA CTT GTT GCC-3',
5'-CCA TAG TGG TCT GCG GAA CCG GTG AGT ACA-3',
5'-ATT GCG AGA TCT ACG GGG CCT GCT ACT CCA-3',
5'-ATA GCG GCC GCC CTC GAT TGC GAG AGC TAC-3',
5'-AAT TCG GGC GGC CGC CAT ACG A-3',
5'-CTT GAT CTA CCT CCA ATC ATT CAA AGA CTC-3',
5'-TCT TCA ACT GGG CAG TAA GAA CAA AGC TCA-3',
5'-AAT TCG CGG CCG CCA TAC GAT TTA GGT GAC ACT ATA GAA T15-3',
5'-TTC GCG GCC GCT ACA GCG GGG GAG ACA T-3',
' -AAT TCG CGG CCG CCA TAC GA-3',
5'-CGA TGA AGG TTG GGG TAA ACA CTC CGG CCT-3',
5'-GAT CCT GGA ATT CTG ATA AGA CCT TAA GAC TAT TTT AA-3',
5'-AAT TTG GGA ATT CCA TAA TGA GAC CCT TAA GGT ATT ACT CAG CT-3',
5'-GAG TGC TCA AGC TTC AAA ACA AAA TGG CTC ACT TTC TAT CCC AGA CAA AGC AGA GT-3',
5'-GAG TGC TCG ACT CAT TAG GGG GAA ACA TGG TTC CCC CGG GAG GCG AA-3',
5'-GAG TGC TCA AGC TTC AAA ACA AAA TGG GGC TCT ACC ACG TCA CCA ATG ATT GCC CTA AC-3',
5'-GAG TGC TCG TCG ACT CAT TAA GGG GAC CAG TTC ATC ATC ATA TCC CAT GCC AT-3',
5'-GAG TGC AGC TTC AAA ACA AAA TGA GCA CGA ATC CTA AAC CTC AAA AAA AAA AC-3',
5'-GAG TGC TCG TCG ACT CAT TAA CCC AAA TTG CGC GAG CTA CGC CGG GGG TCT GT-3',
5'-GAG TGC TCA AGC TTA CAA AAC AAA ATG GCA CCA GGC GCC AAG CAG AAC GTC CAG CTG ATC-3',
5'-GAG TGC TCC TCG AGG TCG ACT CAT TAC TCG GAC CTG TCC CTA TCT TCC AGA TCG CAA CG-3',
5'-GGA TCC GCT AGC GGC GCC AAG CAG AAC GTC CAG CTG ATC AAC ACC-3',
5'-GGA TCC AAG CTT TTA CTC GGA CCT GTC CCT ATC TTC CAG ATC GCA ACG-3',
5'-CAA TCA TAC CTG ACA G-3',
' -GAT AAC CTC TGC CTG A-3',
5'-GCA TGT CAT GAT GTA T-3',
5'-ACA ATA CTG GTG TCA C-3',
5'-CCA GCG GTG GCC TGG TAT TG-3',
5'-TTT GGG TAA GGT CAT CGA TAC CCT TAC GTG-3',
5'-ATA TGC GGC CGC CTT CCG TTG GAC TAA-3',
5'-AAT TCG CGG CCG CCA TAC GAT TTA GGT GAC ACT ATA GAA CCC CCC CCC CCC CCC-3',
5'-CGA CAA GAA AGA CAG A-3',
5'-CGT TGG CAT AAC TGA T-3',
5'-CTC TAT GGC AAT GAG G-3',
5'-AGC TTC GAC GTC ACA T-3',
5'-CTT GAA TTC GCA ATT TGG GTA AGG TCA TCG ATA CCC TTA CG-3',
5'-CTT GAA TTC GAT AGA GCA ATT GCA ACC TTG CGT CGT CC-3',
5'-CTT GAA TTC GGA CGA CGC AAG GTT GCA ATT GCT CTA TC-3',
5'-CTT GAA TTC CAG CCG GTG TTG AGG CTA TCA TTG CAG TTC-3',
5'-TGA ACT ATG CAA CAG G-3',
5'-GGA GTG TGC AGG ATG G-3',
5'-AAG GTT GCA ATT GCT C-3',
5'-ACT AAC AGG ACC TTC G-3',
5'-TAA CGG GTC ACC GCA T-3',
5'-GTA ATA TGG TGA CAG AGT CA-3',
5'-GAT CTC TAG AGA AAT CAA TAT GGT GAC AGA GTC A-3', and
5'-CCC AGC GGC GTA CGC GCT GGA CAC GGA GGT GGC CGC GTC GTG TGG CGG TGT TGT TCT CGT CGG GTT GAT GGC GC-3'.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a divisional of U.S. Ser. No. 08/040,564, filed 31 Mar. 1993; which is a continuation of U.S. Ser. No. 07/566,209, filed on 10 Aug. 1990, now abandoned; which is a continuation-in-part of U.S. Ser. No. 07/505,435 filed 4 Apr. 1990, now abandoned; which is a continuation-in-part of U.S. Ser. No. 07/456,637 filed 21 Dec. 1989, now abandoned, U.S. Ser. No. 07/355,002 filed 18 May 1989, now abandoned, and of U.S. Ser. No. 07/355,961 filed 18 May 1989, now abandoned; wherein said 07/355,002 and 07/355,961 are each a continuation-in-part of U.S. Ser. No. 07/341,334 filed 20 Apr. 1989, now abandoned, which is a continuation-in-part of PCT Application No. PCT/US88/04125 filed 18 Nov. 1988, and 07/325,338 filed 17 Mar. 1989, now abandoned; wherein said PCT/US88/04125 and Ser. No. 07/325,338 are each a continuation-in-part of U.S. Ser. No. 07/271,450 filed 14 Nov. 1988, now abandoned, which is a continuation-in-part of U.S. Ser. No. 07/263,584 filed 26 Oct. 1988, now abandoned, which is a continuation-in-part of U.S. Ser. No. 07/191,263 filed 6 May 1988, now abandoned, which is a continuation-in-part of U.S. Ser. No. 07/161,072 filed 26 Feb. 1988, now abandoned, which is a continuation-in-part of U.S. Ser. No. 07/139,886 filed 30 Dec. 1987, now abandoned, which is a continuation-in-part of U.S. Ser. No. 07/122,714 filed 18 Nov. 1987, now abandoned; the aforementioned applications are, in their entirety, incorporated herein by reference.

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First Opposition to Chiron Japanese Patent Application No. 01-500565, filed by Steven Woodsmall (and translation) with supporting documents.
Reply to First Opposition to Chiron Japanese Patent Application No. 01-500565.
Second Opposition to Chiron Japanese Patent Application No. 01-500565, filed by Akira Yamamoto (and translation) with supporting documents.
Reply to Second Opposition to Chiron Japanese Patent Application No. 01-500565.
Third Opposition to Chiron Japanese Patent Application No. 01-500565, filed by Asako Sudo (and translation) with supporting documents.
Reply to Third Opposition to Chiron Japanese Patent Application No. 01-500565.
Fourth Opposition to Chiron Japanese Patent Application No. 01-500565, filed by Yashimi Tawara (and translation) with supporting documents.
Reply to Fourth Opposition to Chiron Japanese Patent Application No. 01-500565.
Fifth Opposition to Chiron Japanese Patent Application No. 01-500565, filed by Tetsuo Nakamura (and translation).
Reply to Fifth Opposition to Chiron Japanese Patent Application No. 01-500565.
Sixth Opposition to Chiron Japanese Patent Application No. 01-500565, filed by Toren K.K. (and translation) with supporting documents.
Reply to Sixth Opposition to Chiron Japanese Patent Application No. 01-500565.
Seventh Opposition to Chiron Japanese Patent Application No. 01-500565, filed by Sumitomo Metal Industries, Ltd. (and translation) with supporting documents.
Reply to Seventh Opposition to Chiron Japanese Patent Application No. 01-500565.
Eighth Opposition to Chiron Japanese Patent Application No. 01-500565, filed by Hiroshi Iino (and translation) with supporting documents.
Reply to Eighth Opposition to Chiron Japanese Patent Application No. 01-500565.
Ninth Opposition to Chiron Japanese Patent Application No. 01-500565, filed by Kyokuto Seiyaki Kogyo, Inc. (and translation) with supporting documents.
Reply to Ninth Opposition to Chiron Japanese Patent Application No. 01-500565.
Tenth Opposition to Chiron Japanese Patent Application No. 01-500565, filed by United Biomedical, Inc. (and translation) with supporting documents.
Reply to Tenth Opposition to Chiron Japanese Patent Application No. 01-500565.
Eleventh Opposition to Chiron Japanese Patent Application No. 01-500565, filed by Kokusai Shiyaku (and translation) with supporting documents.
Reply to Eleventh Opposition to Chiron Japanese Patent Application No. 01-500565.
Twelfth Opposition to Chiron Japanese Patent Application No. 01-500565, filed by Shigeo Kimura (and translation) with supporting documents.
Reply to Twelfth Opposition to Chiron Japanese Patent Application No. 01-500565.
Thirteenth Opposition to Chiron Japanese Patent Application No. 01-500565, filed by Akzo Neamloze Vennootschap (and translation).
Reply to Thirteenth Opposition to Chiron Japanese Patent Application No. 01-500565.
Fourteenth Opposition to Chiron Japanese Patent Application No. 01-500565, filed by F. Hoffman la Roche AG (and translation) with supporting documents.
Reply to Fourteenth Opposition to Chiron Japanese Patent Application No. 01-500565.
Translation of Decision on Opposition Against Patent Application No. 01-500565, issued Dec. 22, 1995.
Statement of Grounds for Opposition to Australian Patent No. 640,920, filed by Hoffman LaRoche with supporting documents.
Australian Litigation Bundle, for Chiron Australian Patent No. 624,105.
Murex Reply in the Litigation of Chiron Australian Patent No. 624,105.
First Opposition to Chiron European Application No. 0 318 216, filed by Akzo Pharma BV (Jan. 27, 1994) with supporting documents.
Second Opposition to Chiron European Application No. 0 318 216, filed by Murex Diagnostics Limited(Sep. 12, 1994) with supporting documents.
Third Opposition to Chiron European Application No. 0 318 216, filed by United Biomedical (Sep. 15, 1994) with supporting documents.
Fourth Opposition to Chiron European Application No. 0 318 216, filed by Beringwerke (Sep. 15, 1994).
Fifth Opposition to Chiron European Application No. 0 318 216, filed by The Research Foundation for Microbial Diseases (Sep. 14, 1994) with supporting documents.
Sixth Opposition to Chiron European Application No. 0 318 216, filed by F. Hoffman-La Roche AG(Sep. 15, 1994).
J.A. Kemp & Co.(Chiron): Response By Patentee To Notices of Opposition re EP 318 216, Jun. 1995.
Miscellaneous Documentation (believed to have been supplied to Prof. Donald Chisum by Dr. Bradley or the Center for Disease Control).
Daniel W. Bradley v. Chiron Corporation, Civil Action No. 94-4342 Litigation Bundle, with supporting documents.
�U.K. LITIGATION Bundle 1!
�U.K. LITIGATION Bundle 2!
�U.K. LITIGATION Bundle 5!
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�U.K. LITIGATION Bundle 7a!
�U.K. LITIGATION Bundle 7b!
�U.K. LITIGATION Bundle 7c!
�U.K. LITIGATION Bundle 8!
�U.K. LITIGATION Bundle 9!
�U.K. LITIGATION Bundle 9.a.1!
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�U.K. Interlocutory Affidavits and Exhibits, vol. 1!
�U.K. Interlocutory Affidavits and Exhibits, vol. 2!
�U.K. Interlocutory Affidavits and Exhibits, vol. 3!
�U.K. Interlocutory Affidavits and Exhibits, vol. 4!
�U.K. Transcript Speeches and Evidence, vol. 4!
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Related Publications (3)

	Number	Date	Country
	355002	May 1989
	355961	May 1989
	325338	Mar 1989

Divisions (1)

	Number	Date	Country
Parent	40564	Mar 1993

Continuations (1)

	Number	Date	Country
Parent	566209	Aug 1990

Continuation in Parts (11)

	Number	Date
Parent	505435	Apr 1990
Parent	456637	Dec 1989
Parent	341334	Apr 1989
Parent	341334
Parent	271450	Nov 1988
Parent	271450
Parent	263584	Oct 1988
Parent	191263	May 1988
Parent	161072	Feb 1988
Parent	139886	Dec 1987
Parent	122714	Nov 1987

Methods for detecting Hepatitis C virus using polynucleotides specific for same

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