Claims
- 1. A method for detecting the presence of a vancomycin-resistant microorganism in a sample, comprising:
(a) contacting a sample that may have a target nucleic acid molecule with a nucleic acid probe and a cleaving agent, wherein the probe is substantially complementary to a portion of the target and comprises a nucleic acid molecule having the sequence of 5′-CATGATGTGTCGGTAAAATCTGCAATAGAGA-3′ (SEQ ID NO:6); (b) incubating under conditions and for a time sufficient for the formation of a probe:target hybridized duplex and for the probe to be cleaved within the probe:target duplex; (c) recycling the target molecule at least once through step (b); and (d) determining whether at least one probe fragment is released from the complex, thereby detecting the presence of a vancomycin-resistant microorganism.
- 2. The method of claim 1 wherein the probe comprises a nucleic acid molecule having the sequence of 5′-CATGATGTGTCGGTaaaaTCTGCAATAGAGA-3′ (SEQ ID NO:5).
- 3. The method according to claim 1 or claim 2 wherein the cleaving agent is RNase H.
CROSS-REFERENCE TO RELATED APPLICATION
[0001] This application claims the benefit of U.S. Provisional Patent Application No. 60/352,750 filed Jan. 30, 2002, where this provisional application is incorporated herein by reference in its entirety.
Provisional Applications (1)
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Number |
Date |
Country |
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60352750 |
Jan 2002 |
US |