Methods for identifying genomic deletions

BACKGROUND OF THE INVENTION

1. Field of the Invention

This invention relates generally to disease diagnosis and to the identification of disease carriers. More specifically, the present invention provides methods for identifying individuals who are afflicted with or carriers of diseases associated with one or more genomic deletion.

2. Description of the Related Art

Van Buchem's disease (VBD) is a rare autosomal recessive disorder that results in a bone dysplasia referred to as craniotubular hyperostosis. VBD was first described in 1962 as including osteosclerosis of the skull, mandible, clavicles, ribs, and diaphysis of the long bones beginning during puberty and, in some cases, leading to optic atrophy and perceptive deafness from nerve pressure. Van Buchem et al.,

Am. J. Med

. 33:387-397 (1962).

More recently, additional occurrences of VBD have been reported. In 1988, Fryns et al. described a 7.5-year-old boy with VBD. This patient had presented at 2 months of age with left-side peripheral facial nerve palsy but had, at that time, no radiologically visible signs of sclerosis of the skull.

Europ. J. Pediat

. 147:99-100 (1988).

In 1997, Balemans et al. studied 11 VBD patients from a highly inbred and geophraphically isolated Dutch family. Each of these patients shared a common ancestor from 9 preceding generations. By applying a genome wide search for linkage using more than 300 microsatellite markers having an average spacing of 10 cM, these authors found a maximum lod score of 9.33 at theta=0.01 with marker D17S1299 and narrowed the assignment to a region of less than 1 cM between markers D17S1787 and D17S934

. Am. J. Hum. Genet

. 61(Suppl.):A12 (1997); See, also, Van Hul et al.,

Am. J. Hum. Genet

. 62:391-399 (1998).

A related disease sclerosteosis is an autosomal semi-dominant disease that shares some of the clinical symptoms of VBD. The term “sclerosteosis” has been applied to a disorder similar to Van Buchem hyperostosis corticalis generalisata but differing in the radiologic appearance of the bone changes and in the presence of asymmetric cutaneous syndactyly of the index and middle fingers in many cases. In

Handbuch der Kinderheilkunde

351-355 (Opitz, H. et al., Berlin: Springer (pub.), 1967). More specifically, this disease resembles VBD in that it comprises a progressive sclerosing bone dysplasia characterized by generalized osteosclerosis and hyperostosis of the skeleton, affecting mainly the skull and mandible thereby causing facial paralysis and hearing loss. In contrast to VBD, however, sclerosteosis is further characterized by gigantism and hand abnormalities.

The rare genetic mutation responsible for the sclerosteosis syndrome has been localized to the region of human chromosome 17 that encodes a novel member of the TGF-beta binding-protein family (one representative example of which is designated “hSOST”). In 1999, Balemans et al. assigned the locus for sclerosteosis to 17q12-q21 which is the same general region as the locus for VBD.

Am. J. Hum. Genet

. 64:1661-1669 (1999). Due to the clinical similarities between VBD and sclerosteosis, Beighton et al. suggested that these conditions might be caused by mutations within the same gene.

Clin. Genet

25:175-181 (1984). This hypothesis was further supported by the genetic experimentation later performed by Balemans et al. Supra.

Traditional methodologies for identifying genomic deletions such as, for example, restriction fragment length polymorphism (RFLP), fluorescence in situ hybridization (FISH) and Southern blotting permit the identification of individuals who are homozygous for a genomic deletion and, as a consequence, are afflicted with the associated genetic disease. Because these methods are time consuming and/or require high-quality DNA samples or live cells, they are of limited use in the identification of individuals who are heterozygous for and, therefore, carriers of a genetic disease. What is needed in the art are methods that permit the rapid identification of genetic disease carriers, which methods distinguish between individuals who are homozygous for a genomic deletion, individuals who are heterozygous for a genomic deletion and individuals who do not possess a given genomic deletion. As described in detail herein, the present invention fulfills this and other related needs.

BRIEF SUMMARY OF THE INVENTION

The present invention is directed generally to disease diagnosis and to the identification of disease carriers. More specifically, the invention disclosed herein provides methods for identifying chromosomal deletions that are associated with a disease phenotype. Particular methods within the scope of the present invention are directed to the identification of individuals who are afflicted with or carriers for the genomic deletion associated with Van Buchem's disease. By alternate embodiments, the present invention also provides methods having general utility in the detection of a wide variety of diseases characterized by genomic deletions.

The present invention provides, in one embodiment, methods for distinguishing between an individual who is homozygous for a genomic deletion, an individual who is heterozygous for a genomic deletion and an individual who is negative for a genomic deletion.

A first method comprises: (a) obtaining a sample of genomic DNA from an individual; (b) performing a first amplification reaction with a first oligonucleotide primer pair comprising a first oligonucleotide primer and a second oligonucleotide primer wherein the first oligonucleotide primer is complementary to the nucleotide sequence upstream of the genomic deletion and the second oligonucleotide primer is complementary to the nucleotide sequence downstream of said genomic deletion; (c) performing a second amplification reaction with a second oligonucleotide primer pair comprising a third oligonucleotide primer and a fourth oligonucleotide primer wherein the third oligonucleotide primer is complementary to the nucleotide sequence either upstream or downstream of the genomic deletion and the fourth oligonucleotide primer is complementary to the nucleotide sequence comprising the genomic deletion; and (d) detecting the product of the amplification reactions of (b) and (c).

By this first type method, a positive amplification reaction of (b) and a negative amplification reaction of (c) indicates an individual who is homozygous for the large genomic deletion; a positive amplification reaction of (b) and a positive amplification reaction of (c) indicates an individual who is heterozygous for the large genomic deletion; and a negative amplification reaction of (b) and a positive amplification reaction of (c) indicates an individual who is negative for the large genomic deletion.

A second method comprises: (a) obtaining a sample of genomic DNA from said individual; (b) performing an amplification reaction employing at least two oligonucleotide primer pairs in which an oligonucleotide primer is common to both said primer pairs, wherein a first primer pair has a first oligonucleotide primer complementary to a nucleotide sequence that flanks said genomic deletion upstream of said genomic deletion and a second oligonucleotide primer complementary to a nucleotide sequence that flanks said genomic deletion downstream of said genomic deletion, and a second primer pair has third oligonucleotide primer complementary to a nucleotide sequence within said genomic deletion and either said first or second oligonucleotide primer; and (c) detecting an amplified product of said amplification reaction.

By this second method, a positive amplification reaction of said first primer pair and a negative amplification reaction of said second primer pair indicates an individual that is homozygous for said large genomic deletion; a positive amplification reaction of said first primer and a positive amplification reaction of said second primer pair indicates an individual that is heterozygous for said large genomic deletion; and a negative amplification reaction of said first primer pair and a positive amplification reaction of said second primer pair indicates an individual that is negative for said large genomic deletion.

Both these methodologies will find utility in the detection of large genomic deletions comprising at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 40 or 50 kb. By some embodiments, the presence of the large genomic deletion is indicative of an individual who is either afflicted with or a carrier of a genetic disease. The genetic disease is exemplified herein by Van Buchem's disease, but the methods are suitable for any disease characterized by large genomic deletions.

In further embodiments, the present invention provides a first method for identifying individuals who are afflicted with or carriers of Van Buchem's disease. This first method comprises (a) obtaining a sample of genomic DNA from an individual; (b) performing a first amplification reaction with a first oligonucleotide pair primer having a first oligonucleotide primer and a second oligonucleotide primer wherein the first oligonucleotide primer is complementary to the nucleotide sequence upstream of the 51,719 bp sequence depicted in SEQ ID NO:2 which sequence corresponds to nucleotides 5,798 through 57,516 of the 92,149 bp nucleic acid sequence depicted in SEQ ID NO:1 and the second oligonucleotide primer is complementary to the nucleotide sequence downstream of the 51,719 bp sequence depicted in SEQ ID NO:2; and (c) performing a second amplification reaction with a second oligonucleotide pair primer comprising a third oligonucleotide primer and a fourth oligonucleotide primer wherein the third oligonucleotide primer is complementary to the nucleotide sequence either upstream or downstream of the 51,719 bp sequence depicted in SEQ ID NO:2 and the fourth oligonucleotide is complementary to the nucleotide sequence within the 51,719 bp sequence depicted in SEQ ID NO:2; and (d) detecting the product of the amplification reactions of (b) and (c).

By this first method, a positive amplification reaction of (b) and a negative amplification reaction of (c) indicates an individual afflicted with Van Buchem's disease; a positive amplification reaction of (b) and a positive amplification reaction of (c) indicates an individual who is a carrier of Van Buchem's disease; and a negative amplification reaction of (b) and a positive amplification reaction of (c) indicates an individual that is neither afflicted with nor a carrier of Van Buchem's disease.

Exemplary first oligonucleotide primer pairs may be selected from the group consisting of 12952/VBspan1 (SEQ ID NO:84/SEQ ID NO:85), Span1F/Span1R (SEQ ID NO:86/SEQ ID NO:87), Span2F/Span2R (SEQ ID NO:88/SEQ ID NO:89) and Vbspan2/Vbspan1 (SEQ ID NO:104/SEQ ID NO:85). Exemplary second oligonucleotide pairs may be selected from the group consisting of 12952/Wt1R (SEQ ID NO:84/SEQ ID NO:90), Wt2F/Wt2R (SEQ ID NO:91/SEQ ID NO:92), Wt3F/Wt3R (SEQ ID NO:93/SEQ ID NO:94) and VBspan2/VBint1 (SEQ ID NO:105/SEQ ID NO:102).

A second method comprises: (a) obtaining a sample of genomic DNA from said individual; (b) performing a polymerase chain reaction employing at least two oligonucleotide primer pairs in which an oligonucleotide primer is common to both said primer pairs, wherein a first primer pair has a first oligonucleotide primer that is complementary to a nucleotide sequence upstream of the 51,719 bp sequence provided in SEQ ID NO:2 and a second oligonucleotide primer that is complementary to a nucleotide sequence downstream of the 51,719 bp sequence provided in SEQ ID NO:2, and a second primer pair has a third oligonucleotide primer that is complementary to a nucleotide sequence within said genomic deletion and either said first or second oligonucleotide primer; and (c)detecting an amplified product of said amplification reaction.

By this second method, a positive polymerase chain reaction of said first primer pair and a negative polymerase chain reaction of said second primer pair indicates an individual afflicted with Van Buchem's disease; a positive polymerase chain reaction of said first primer pair and a positive polymerase chain reaction of said second primer pair indicates an individual that is a carrier of Van Buchem's disease; and a negative polymerase chain reaction of said first primer pair and a positive polymerase chain reaction of said second primer pair indicates an individual that is neither afflicted with nor a carrier of Van Buchem's disease.

Exemplary first oligonucleotide primer pair is selected from the group consisting of 12952/VBspan1 (SEQ ID NO:84/SEQ ID NO:85), Span1F/Span1R (SEQ ID NO:86/SEQ ID NO:87), Span2F/Span2R (SEQ ID NO:88/SEQ ID NO:89), Wt2F/VBspan1 (SEQ ID NO:91/SEQ ID NO:85) and VBspan2/VBspan1 (SEQ ID NO:104/SEQ ID NO:85).

Still further embodiments of the present invention provide alternative methods for identifying individuals afflicted with Van Buchem's disease. Exemplary methods comprise the steps of performing an amplification reaction with a pair of oligonucleotides selected from the region between nucleotide 1 and nucleotide 51,719 of SEQ ID NO:2 wherein the absence of an amplification product indicates an individual homozygous for Van Buchem's disease. Exemplary oligonucleotide pairs are selected from the group consisting of Del1F/Del1R (SEQ ID NO:95/SEQ ID NO:96), Del2F/Del2R (SEQ ID NO:97/SEQ ID NO:98), and Del3F/Del3R (SEQ ID NO:99/SEQ ID NO:100).

Other embodiments provide methods for identifying an individual who is homozygous for Van Buchem's disease comprising the step of detecting a deletion in human chromosome 17 at 17q21 between nucleotide 5,798 and nucleotide 57,516 as depicted in SEQ ID NO:1.

By still other embodiments are provided methods for detecting an individual who is afflicted with or a carrier of Van Buchem's disease which methods comprise detecting the nucleotide sequence spanning the deletion breakpoint as depicted in FIG.

2

and SEQ ID NO:101. By these methods, the presence of a deletion breakpoint indicates an individual who is either afflicted with or a carrier of Van Buchem's disease. Exemplary nucleotide sequences spanning the deletion breakpoint comprise the nucleotide sequence 5′-ACCATGCCCGGCTAAT-3′ (SEQ ID NO:102); the nucleotide sequence 5′-CTACCATGCCCGGCTAATTTT-3′ (SEQ ID NO:103); and the nucleotide sequence 5′-TGGGATTACAGGTGCATGCTACCATGCCCGGCTAATTTTTTTGTA TTTTTTTAGTA-3′ (SEQ ID NO:101).

In still further embodiments of the present invention are provided isolated polynucleotides. Preferred polynucleotides comprise at least 10, 15, 20, 25, 30, 40, 50, 100, 250 or 500 contiguous nucleotides of the nucleic acid depicted in SEQ ID NO:1. Alternatively, isolated polynucleotides according to the present invention hybridize under moderately stringent conditions to the nucleic acid depicted in SEQ ID NO:1 or the complement thereof. Preferred moderately stringent conditions comprise 2×SSC, 0.1% SDS at 65° C.

Other embodiments provide isolated polynucleotides that comprise at least 10, 15, 20, 25, 30, 40, 50, 100, 250 or 500 nucleotides of one of the amplicons comprising the predicted exons within human chromosome 17 at 17q21 which amplicons are selected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, and SEQ ID NO:17.

Still further embodiments of the present invention provide diagnostic kits for distinguishing between an individual who is homozygous for a large genomic deletion, an individual who is heterozygous for a large genomic deletion and an individual who is negative for a large genomic deletion.

A first kit comprises: (a) a first oligonucleotide primer pair comprising a first oligonucleotide primer and a second oligonucleotide primer, wherein said first oligonucleotide primer is complimentary to a nucleotide sequence upstream of said genomic deletion and said second oligonucleotide primer is complementary to a nucleotide sequence downstream of said genomic deletion; and (b) a second oligonucleotide primer pair comprising a third oligonucleotide primer and a fourth oligonucleotide primer wherein said third oligonucleotide primer is complementary to a nucleotide sequence either upstream or downstream of said genomic deletion and said fourth oligonucleotide primer is complementary to a nucleotide sequence within said genomic deletion. These types of diagnostic kit can further comprises instructions for distinguishing between an individual who is homozygous for a large genomic deletion, an individual who is heterozygous for a large genomic deletion and an individual who is negative for a large genomic deletion.

A second kit comprises: (a) a first primer pair having a first oligonucleotide primer that is complementary to a nucleotide sequence that flanks said genomic deletion upstream of said genomic deletion and a second oligonucleotide primer that is complementary to a nucleotide sequence that flanks said genomic deletion downstream of said genomic deletion; and (b) a second primer pair having a third oligonucleotide primer that is complementary to a nucleotide sequence within said genomic deletion and either said first or second oligonucleotide primer.

The genomic deletion mentioned can be associated with Van Buchem's disease. In which case one type of diagnostic kit for identifying a carrier of Van Buchem's disease comprises: (a) a first oligonucleotide primer pair comprising a first oligonucleotide and a second oligonucleotide wherein said first oligonucleotide is complementary to a nucleotide sequence upstream of the 51,719 bp sequence depicted in SEQ ID NO:2 and said second oligonucleotide is complementary to a nucleotide sequence downstream of the 51,719 bp sequence depicted in SEQ ID NO:2; and (b) a second oligonucleotide primer pair comprising a third oligonucleotide and a fourth oligonucleotide wherein said third oligonucleotide is complementary to either the nucleotide sequence upstream or downstream of said 51,719 bp sequence depicted in SEQ ID NO:2 and said fourth oligonucleotide is complementary to a nucleotide sequence within said 51,719 bp sequence depicted in SEQ ID NO:2. These diagnostic kits can further comprise instructions for identifying a carrier of Van Buchem's disease.

A second kit comprises: (a) a first oligonucleotide pair having a first oligonucleotide primer that is complementary to a nucleotide sequence upstream of the 51,719 bp sequence provided in SEQ ID NO:2 and a second oligonucleotide primer that is complementary to a nucleotide sequence downstream of the 51,719 bp sequence depicted in SEQ ID NO:2; and (b) a second oligonucleotide pair having a third oligonucleotide primer that is complementary to a nucleotide sequence within said 51,719 bp sequence provided in SEQ ID NO:2 and either said first or second oligonucleotide primer.

BRIEF DESCRIPTION OF THE SEVERAL VIEWS OF DRAWINGS

FIG. 1A

illustrates results obtained from microsatellite typing and exon sequencing of the approximately 90 kb region between the MEOXI and SOST genes within human chromosome region 17q21.

FIG. 1B

shows the about 53 bp genomic deletion as well as illustrates the nucleotide sequence spanning the deletion breakpoint (SEQ ID NO:101) in nucleic acid sequences obtained from individuals afflicted with Van Buchem's disease.

FIG. 2

illustrates expected results from the polymerase chain reactions utilizing exemplary oligonucleotide primer pairs in the first-described method, where the nucleic acid sample is obtained from an individual who is (1) a non-carrier for Van Buchem's disease; (2) heterozygous for, and therefore a carrier of, Van Buchem's disease; or (3) homozygous for, and therefore afflicted with, Van Buchem's disease.

FIG. 3

illustrates expected results from the polymerase chain reaction utilizing exemplary oligonucleotide primer pairs in the second-described method, where the nucleic acid sample is obtained from an individual who is (1) a non-carrier for Van Buchem's disease; (2) heterozygous for, and therefore a carrier of, Van Buchem's disease; or (3) homozygous for, and therefore afflicted with, Van Buchem's disease.

DETAILED DESCRIPTION OF THE INVENTION

As part of the present invention, it was discovered, through haplotype analysis of a number of VBD patients carrying recombinant chromosomes, that the VBD gene localized to a critical region of less than 1 Mb, between the polymorphic markers BP12574 (D17S2250) and JM6307 (D17S2253) (see Example 1). Further microsatellite typing, as well as characterization of the candidate genes within the 1 Mb region through exon sequencing, revealed a specific chromosomal aberration that segregated absolutely with VBD (see Example 3).

As discussed in detail in Example 3, two independent experimental approaches to identifying the VBD candidate region revealed the presence of a chromosomal deletion associated with VBD. First, the oligonucleotide pair D17S1789 (see FIG.

1

and Table 3) did not permit the PCR amplification of a DNA fragment from genomic DNA isolated from VBD patients but did facilitate the amplification of a fragment from normal individuals. Second, nucleotide sequencing and computational analysis, with the GENSCAN exon prediction algorithm of Burge et al., of a 92,149 bp fragment (SEQ ID NO:1) within the human 17q21 chromosomal locus revealed the presence of 15 putative exon sequences.

J. Mol. Biol

. 268:78-94 (1997). As presented in FIG.

1

and as discussed in detail in Example 3, 12 of these 15 exon fragments did not amplify from genomic DNA isolated from VBD-afflicted individuals.

In total, these results demonstrated for the first time the presence of a genomic deletion within human chromosome 17q21 that segregates absolutely with Van Buchem's disease. Furthermore, nucleotide sequencing of the region spanning the deletion breakpoint (SEQ ID NO:101) revealed that approximately 52 kb of contiguous genomic DNA is invariably deleted from chromosome 17 at position 17q21 in individuals afflicted with VBD. The sequence of the 52 kb region is presented herein as SEQ ID NO:2.

Isolated Polynucleotides

As noted above, the present invention provides isolated polynucleotides comprising at least 10, 15, 20, 25, 30, 40, 50, 100, 250, 500, 600, 700, 800 up to and including the 92,101 base pairs (bp) nucleotide sequence of human chromosome 17 at 17q21 disclosed herein as SEQ ID NO:1. Also provided by the present invention are isolated polynucleotides comprising at least 10, 15, 20, 25, 30, 40, 50, 100, 250, 500 bp up to and including the 52 kb sequence of 17q21 that is deleted in individuals who are either afflicted with or carriers of Van Buchem's disease (i.e. SEQ ID NO:2). As well, the present invention provides polynucleotides comprising at least 10, 15, 20, 25, 30, 40, 50, 100, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850 or 890 bp of any of the exons depicted in SEQ ID NOs: 3-17. Other embodiments of the present invention provide oligonucleotide probes of at least 10, 15, 20, 25, 30, 40, 50, 100, 250, 500, 600, 750, 1000, 1050, 1100, 1200 or 1300 that hybridize under moderately stringent conditions to the nucleotide sequence of SEQ ID NO:1 or complements thereto. Exemplary oligonucleotide probes according to the present invention include those designated herein by SEQ ID NOs:28-100.

As used herein, the phrase “isolated polynucleotide” refers to a DNA molecule that has been isolated free of total genomic DNA of a particular species. As will be understood by those skilled in the art, the isolated polynucleotides of the present invention can include genomic sequences, extra-genomic and plasmid-encoded sequences and smaller engineered gene segments that express, or may be adapted to express, proteins, polypeptides, peptides and the like. Such segments may be naturally isolated, or synthesized de novo.

Thus, “isolated,” as used herein, means that the polynucleotide is substantially separated away from other genomic sequences and that the polynucleotide does not contain large portions of unrelated coding DNA, such as large chromosomal fragments. Of course, this refers to the polynucleotide as originally isolated, and does not exclude genes or coding regions later added to the segment.

As will be recognized by the skilled artisan, isolated polynucleotides may be single-stranded or double-stranded, and may be DNA or RNA molecules. Additional coding or non-coding sequences may, but need not, be present within a polynucleotide of the present invention, and a polynucleotide may, but need not, be linked to other molecules and/or support materials.

It is also contemplated that the present invention encompasses polynucleotides having substantial identity to any of the sequences disclosed herein, for example the present invention encompasses those isolated polynucleotides comprising at least 50% or more sequence identity, preferably at least 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% or higher, sequence identity compared to a polynucleotide sequence of this invention using the methods described herein, (e.g., BLAST, described in Altschul et al.,

J. Mol. Biol

. 215:403-410 (1990)). For the purposes of this invention, a preferred method of calculating percent identity is the Smith-Waterman algorithm, using the following. Global DNA sequence identity must be at least 50% as determined by the Smith-Waterman homology search algorithm as implemented in MPSRCH program (Oxford Molecular) using an affine gap search with the following search parameters: gap open penalty, 12; and gap extension penalty, 1.

In additional embodiments, the present invention provides isolated polynucleotides comprising various lengths of contiguous stretches of sequence identical to or complementary to one or more of the sequences disclosed herein. For example, isolated polynucleotides are provided by this invention that comprise at least about 10, 15, 20, 25, 30, 40, 50, 100, 250, 500, 600, 750, 1000, 1050, 1100, 1200 or 1300 or more contiguous nucleotides of one or more of the sequences disclosed herein.

The polynucleotides of the present invention, or fragments thereof, may be combined with other DNA sequences, such as promoters, polyadenylation signals, additional restriction enzyme sites, multiple cloning sites, other coding segments, and the like, such that their overall length may vary considerably. It is therefore contemplated that a nucleic acid fragment of almost any length may be employed, with the total length preferably being limited by the ease of preparation and use in the intended recombinant DNA protocol.

In other embodiments, the present invention is directed to polynucleotides that are capable of hybridizing under moderately stringent conditions to a polynucleotide sequence provided herein, or a fragment thereof, or a complementary sequence thereof. Hybridization techniques are well known in the art of molecular biology. For purposes of illustration, suitable moderately stringent conditions for testing the hybridization of a polynucleotide of this invention with other polynucleotides includes hybridization in a solution of 2×SSC, 0.1% SDS, 1.0 mM EDTA (pH 8.0) at 65° C.

In still other embodiments of the present invention, the polynucleotide sequences provided herein may be employed as primers in the amplification methodology disclosed herein, infra. As such, it is contemplated that polynucleotides comprising at least about 10 nucleotides that have the same sequence as, or that are complementary to, a 10 nucleotide long contiguous sequence disclosed herein will find utility as an amplification primer. Longer contiguous identical or complementary sequences, e.g., those of about 15, 20, 25, 30, 40, 50, 100, 250, 500, 600, 750, 1000, 1050, 1100, 1200 or 1300 or more will also be of use in certain embodiments.

Small polynucleotide segments or fragments may be readily prepared by, for example, directly synthesizing the fragment by chemical means, as is commonly practiced using an automated oligonucleotide synthesizer. Also, fragments may be obtained by application of nucleic acid reproduction technology, such as the PCR™ technology of U.S. Pat. No. 4,683,202 (incorporated herein by reference), by introducing selected sequences into recombinant vectors for recombinant production, and by other recombinant DNA techniques generally known to those of skill in the art of molecular biology. See, e.g., Ausubel et al. (eds.),

Short Protocols in Molecular Biology

(3

rd

Ed., John Wiley & Sons 1995).

Methodologies for Detecting Van Buchem's Disease Carriers

As noted above, the present invention provides methods for detecting carriers of Van Buchem's disease. More specifically, within certain embodiments, these methods permit the differentiation between individuals who are homozygous for, and therefore afflicted with, VBD; individuals who are heterozygous for, and therefore carriers of, VBD; and individuals who are normal with respect to VBD. As described in detail herein, infra, the presently disclosed methodology will find general utility in the detection of a wide variety of diseases associated with genomic deletions of any length.

The methodology according to the present invention relies, in part, on the inability of various DNA polymerases that are typically utilized in amplification reactions to amplify DNA fragments beyond a maximum nucleotide length. As discussed above, the genomic locus associated with Van Buchem's disease is associated with a large chromosomal deletion of approximately 52 kb at 17q21. Consequently, this deletion results in the juxtapositon of nucleotide sequences present in the genomic DNA of non-VBD-afflicted individuals that are normally separated by greater than 52 kb. Because DNA polymerases normally utilized for the amplification of DNA fragments cannot amplify a 52 kb fragment, a pair of oligonucleotide primers that bind to nucleotide sequences upstream or downstream of this 52 kb region will not permit the amplification of DNA from a non-VBD-afflicted individual. In contrast, however, the same pair of oligonucleotide primers will facilitate the amplification of a DNA fragment from genomic DNA isolated from either a VBD-afflicted individual or from a VBD carrier.

The figures illustrate the genomic DNA corresponding to human chromosome 17 at 17q21 between the MEOXI gene and exon 4. The genomic DNA from non-VBD-afflicted individuals comprises a 52 kb fragment described above while genomic DNA from VBD-afflicted individuals lack this 52 kb region.

FIGS. 2 and 3

also illustrate exemplary oligonucleotide primers that hybridize to various regions within the 17q21 locus. Oligonucleotide primers designated VBspan2 and VBspan1/12953, and 12952 and VBspan1/12953 hybridize to nucleotide sequences, on complementary strands, that flank this 52 kb region in normal genomic DNA and, consequently, that flank the deletion breakpoint in genomic DNA from VBD-afflicted individuals. Because of the intervening 52 kb fragment in normal genomic DNA, neither the VBspan2/VBspan1 oligonucleotide primer pair nor the 12952/12953 oligonucleotide primer pair will permit the amplification (e.g., by PCR; see infra) of a DNA fragment from non-VBD-afflicted individuals. In contrast, the VBspan2/VBspan1 oligonucleotide pair primer will facilitate the amplification of a 642 bp fragment, and the 12952/12953 oligonucleotide pair primer will facilitate the amplification of a 798 bp fragment from the DNA of a VBD-afflicted individual.

The methodology according to the present invention additionally permits the differentiation between an individual who is heterozygous for the 52 kb genomic deletion and therefore a carrier of VBD; an individual who is homozygous for the 52 kb genomic deletion and therefore afflicted with VBD; and an individual who is neither a carrier of nor afflicted with VBD. This is achieved by the performance of a second amplification reaction employing a second pair of oligonucleotide primers wherein a third oligonucleotide primer hybridizes to a region of the genomic DNA flanking, either upstream or downstream of, the 52 kb sequence and a fourth oligonucleotide primer hybridizes to a region of the genomic DNA within the 52 kb sequence.

Alternatively, this can be performed using a single multiplex reaction employing a second pair of oligonucleotide primers wherein a third oligonucleotide primer hybridizes to a region of the genomic DNA within the 52 kb sequence and the other oligonucleotide primer is one of the primers from the first oligonucleotide primer pair. Such an oligonucleotide pair is exemplified in

FIG. 3

by the VBspan2/VBint1 (SEQ ID NO:104/SEQ ID NO:105) oligonucleotide pair (SEQ ID NO:91/SEQ ID NO:92). As depicted in

FIG. 3

, a PCR reaction employing this oligonucleotide pair will amplify a 720 bp DNA fragment from the genomic DNA from an individual who is neither afflicted nor a VBD-carrier and from the genomic DNA of a heterozygous VBD-carrier but will not amplify a DNA fragment from the genomic DNA of a homozygous VBD-afflicted individual.

The methodologies according to the present invention permit an alternative approach to differentiating between an individual who is heterozygous for the 52 kb genomic deletion and therefore a carrier of VBD; an individual who is homozygous for the 52 kb genomic deletion and therefore afflicted with VBD; and an individual who is neither a carrier of nor afflicted with VBD, as described in Examples 4 and 5.

In Example 4, this can be achieved using at least two amplification reactions. In Example 5, this can be achieved by the performance of a single multiplex amplification reaction employing at least two oligonucleotide primer pairs, in which one oligonucleotide is common to both pairs. Thus the amplification reaction includes three oligonucleotide primers in total.

Thus, by the present invention provides two methodologies that permit the identification of VBD carriers by allowing the differentiation between an individual who is homozygous for the 52 kb genomic deletion and, therefore, afflicted with VBD; an individual who is heterozygous for the 52 kb genomic deletion and, therefore, a carrier for VBD; and an individual who is normal with respect to the 52 kb deletion and, therefore, neither a carrier of nor afflicted with VBD.

While the present invention discloses the nucleotide sequences of specific exemplary oligonucleotide primers that may be utilized according to the methodologies disclosed herein, it will be apparent to the skilled artisan that alternative oligonucleotide primers may be designed based on the nucleotide sequence depicted in SEQ ID NO:1 which primers are entirely within the scope of the present invention. Thus, for example, suitable oligonucleotide primers according to the present invention include polynucleotides comprising at least 10, 15, 20, 25, 30, 40, 50, 60, 75, 90 or 100 contiguous nucleotides of the nucleic acid depicted in SEQ ID NO:1.

It will also be apparent to one skilled in the art that the choice of reaction conditions and DNA polymerases employed for performing the amplification reactions may be varied in accordance with such parameters as the nucleotide sequence of the oligonucleotide primers and the distance between the primer pairs. Exemplary conditions for 25 ul reactions according to the present methodologies include, but are not limited to, the following: 10 ng genomic DNA; 60 mM TrisHCl pH 7.5; 15 mM ammonium sulfate; 2.5 mM magnesium chloride; 0.4 mM dNTP; and 0.2 uM of each oligonucleotide primer. A typical amplification protocol involves an initial denaturation at 94° C. for 3 min.; 40 cycles of 94° C. for 1 min., 68° C. for 1 min. and 72° C. for 1 min.; and a final extension reaction at 72° C. for 5 min. Amplicons may be resolved by electrophoresis on a 1% agarose gel run in 1×TAE buffer and may be detected by staining with ethidium bromide. Further exemplary conditions for 50 μl reactions according to the present methodologies include, but are not limited to, the following: 20 ng genomic DNA, 0.4 μM each of VBspan1 and VBint1, 0.8 μM of VBspan2, 0.2 mM of each dNTP, 120 mM TrisHCl, pH 7.5, 30 mM ammonium sulfate, 5.0 mM magnesium chloride and 2.5U Taq polymerase (Roche Molecular Biochemicals, Indianopolis, Ind.). A further typical amplification protocol involves an initial denaturation at 94° C. for 3 min, 40 cycles of 94° C. for 30 sec, 63° C. for 30 sec, 72° C. for 2 min and a final extension of 72° C. for 5 min. Amplicons may be resolved by electrophoresis on a 2.5% agarose gel run in 1×TAE buffer and may be detected by staining with ethidium bromide.

Genomic DNA may be isolated by standard procedures that are readily available to those of skill in the art. Representative methodology are provided, for example, in Sambrook et al.,

Molecular Cloning: A Laboratory Manual

(Cold Spring Harbor Laboratories, Cold Spring Harbor, N.Y., 1989) and in Ausubel et al. (eds.),

Short Protocols in Molecular Biology

(4

th

Ed., John Wiley & Sons 1999).

General methodology for performing the polymerase chain reaction (PCR™) are described in detail in U.S. Pat. Nos. 4,683,195; 4,683,202 and 4,800,159, each of which is incorporated herein by reference in its entirety. Briefly, in PCR™, two primers are prepared which have sequences that are complementary to regions on opposite complementary strands of the target sequence. An excess of deoxynucleoside triphosphates is added to a reaction mixture along with a DNA polymerase (e.g., Taq polymerase; see, infta, for exemplary alternative polymerases). If the target sequence is present in a sample, the primers will bind to the target and the polymerase will cause the primers to be extended along the target sequence by adding on nucleotides. By raising and lowering the temperature of the reaction mixture, as indicated, supra, the extended primers will dissociate from the target to form reaction products, excess primers will bind to the target and to the reaction product and the process is repeated.

In addition to the polymerase chain reaction, it is contemplated that alternative amplification procedures that are readily available in the art may be employed in the methodology disclosed herein. For example, another suitable method for amplification is the ligase chain reaction (LCR) and is disclosed in Eur. Pat. Appl. Publ. No. 320,308 (incorporated herein by reference). Briefly, in LCR, two complementary probe pairs are prepared, and in the presence of the target sequence, each pair will bind to opposite complementary strands of the target such that they abut. In the presence of a ligase, the two probe pairs will link to form a single unit. By temperature cycling, as in PCR™, bound ligated units dissociate from the target and then serve as “target sequences” for ligation of excess probe pairs. For a related technique, see also, U.S. Pat. No. 4,883,750, incorporated herein by reference in its entirety.

Alternatively, other methods may be similarly employed in place of the polymerase chain reaction for amplification of nucleic acid sequences include the following: (1) Qbeta Replicase, PCT Intl. Pat. Appl. Publ. No. PCT/US87/00880; (2) the isothermal amplification method of Walker et al.; (3) strand displacement amplification (SDA); (4) repair chain reaction (RCR); (5) cyclic probe reaction (CPR); see, also, Great Britain Pat. Appl. No. 2 202 328, and in PCT Intl. Pat. Appl. Publ. No. PCT/US89/01025; (7) transcription-based amplification systems (TAS) Kwoh et al., 1989; PCT Intl. Pat. Appl. Publ. No. WO 88/110315; (8) Eur. Pat. Appl. Publ. No. 329,822; (9) PCT Intl. Pat. Appl. Publ. No. WO 89/06700; (10) “RACE” (Frohman, 1990); (11) “one-sided PCR” (Ohara, 1989). Each of these methods is incorporated herein by reference.

The choice of DNA polymerase to be employed in the polymerase chain or other amplification reaction can also be determined by the skilled artisan without undue experimentation. As discussed above, the major consideration in selecting an appropriate polymerase is the maximum length of DNA fragment that may be amplified. Such a determination may be achieved initially by reference to the guidelines set forth by the individual manufacturers and ultimately through empirical means by testing stepwise increases in the length of DNA fragment amplified. Additionally or alternatively, the precise reaction condition parameters such as, for example, salt concentration, divalent cation employed and reaction temperature may all be varied in order to adjust, as desired, the length of DNA fragment that may be amplified. The determination of optimal reaction parameters is wholly within the expertise of the skilled artisan.

Exemplary polymerases that may be employed in the present methodology include the various DNA polymerases, and variants thereof, isolated from thermostable bacteria such as the following: Taq, Vent and Deep Vent DNA polymerases (New England BioLabs; Beverly, Mass.); AdvanTaq™, AdvanTaq Plus™, and Advantage

R

2 (Clontech; Palo Alto, Calif.); SuperTaq (Ambion; Austin, Tex.); Tth and Pwo polymerases Roche Molecular Biochemicals; Indianapolis, Ind.); AmpliTaq (PE Biosystems; Foster city, Calif.); and Taq2000 and Pfu DNA polymerases (Stratagene; La Jolla, Calif.).

General Methods for Detection of Large Genomic Deletions

The methodology disclosed herein, supra, for the detection of the large genomic deletion associated with Van Buchem's disease and carriers thereof may be applied broadly, and therefore will find general utility, in the detection of a wide variety of diseases associated with large genomic deletions. In short, the present methods take advantage of the inability of commonly available DNA polymerases to amplify nucleic acid fragments above a certain maximum size when employed in a polymerase chain or other amplification reaction.

Thus, as noted above, the present invention provides general methodologies for distinguishing between an individual who is homozygous for a large genomic deletion, an individual who is heterozygous for a large genomic deletion and an individual who is negative for a large genomic deletion. These methods are discussed in detail in Examples 4 and 5.

As used herein, the phrase “large genomic deletion” refers to those deletions that result from the loss of a contiguous stretch of genomic DNA of such a length that it cannot be amplified in an amplification reaction as disclosed herein, supra. One skilled in the art will recognize, therefore, that the phrase “large genomic deletion” will vary depending on the precise reaction conditions and DNA polymerases employed in the amplification reactions. Generally, the phrase “large genomic deletion” may refer to deletions of at least 2 contiguous kilobases (kb) of genomic DNA. Alternatively, a “large genomic deletion” refers to deletions of at least 3, 4, 5, 6, 7, 8, 9, 10 or more contiguous kb of genomic DNA. Also included within the definition of “large genomic deletions” are deletions of at least 15, 20, 25, 30, 40 or 50 contiguous kb of genomic DNA.

EXAMPLES

The following experimental examples are offered by way of illustration, not limitation.

Example 1

The Van Buchem's Disease Locus Localizes to a Region of Less Than 1 Mb

This example discloses that haplotype analysis of a number of Van Buchem's Disease patients carrying recombinant chromosomes localized the disease to a critical region of less than 1 Mb in a region between the polymorphic markers BP12574 (D17S2250) and JM6307 (D17S2253).

The Van Buchem's Disease region was previously localized to the ˜1 cM interval between polymorphic markers D17S1787 and D17S1861 on chromosome 17q12-21. To refine the interval further, genomic DNA samples were obtained from 15 affected individuals, 4 of whom had not been previously analyzed. A number of microsatellite markers from chromosome 17q12-21 were used to analyze the DNA samples. Patient DNA samples were genotyped using PCR amplification of polymorphic microsatellite markers. The microsatellite markers selected (see Table 1) are described in public databases or were developed using the SPUTNIK algorithm on sequence obtained from a BAC contig across the van Buchem disease/selerosteosis region (Brunkow et al.,

Am. J. Hum. Genet

. 68:577-584 (2001)), D17S1787 to D17S1861. PCR products were labeled with infrared IRDyes™ using an M13 tailing approach as described in Oetting et al.,

Genomics

30:450-458 (1995) and were resolved on a LiCor 4000 DNA sequencer. Allele determinations were made using the SAGA genotyping analysis software (University of Washington) or by a trained eye.

Using this large set of novel polymorphic markers derived from a BAC contig across the D17S1787 and D17S1861 interval, a large region of homozygosity across the disease locus was observed. Furthermore, all affected individuals (including 12-15) shared a common disease haplotype (Table 1). Individuals 2, 3, 6-8 and 12-15 were nonrecombinant across the entire D17S1787-D17S1861 interval, while individuals 1, 4, 5 and 9, 10, 11 carried recombinant chromosomes. Accordingly, the disease locus was refined to the <1 Mb region between BP12574 (D17S2250) and JM6307 (D17S2253) (see Table 1).

TABLE 1

Marker genotypes of affected individuals for selected markers at the van Buchem disease

locus on chromosome

17q12-q21

CONSENSUS

CONSENSUS

DUTCH VAN BUCHEM DISEASE GENOTYPES

VBD

SCLEROSTEOSIS

AFFECTED INDIVIDUAL

HAPLOTYPE

HAPLOTYPE

MARKER

1

4

5

9

10

11

(DUTCH)

(AFRIKANER)

D17S1787

4

4

4

4

4

4

4

8

4

8

4

8

4

1

D17S2231

4

4

4

4

4

4

4

11

4

11

4

11

4

6

D17S1793

4

4

4

4

4

4

4

7

4

7

4

7

4

4

BP7060

11

11

11

11

11

11

11

12

11

12

11

12

11

11

BP7872

2

2

2

2

2

2

2

3

2

3

2

3

2

2

D17S855

6

6

6

6

6

6

6

8

6

8

6

8

6

7

BP12568

1

1

1

1

1

1

1

3

1

3

1

3

1

ND

BP12574

3

3

3

3

3

3

1

3

1

3

1

3

3

ND

(D17S2250)

BP12578

2

2

2

2

2

2

2

2

2

2

2

2

2

ND

BP6991

3

3

3

3

3

3

3

3

3

3

3

3

3

3

D17S1789

Δ

Δ

Δ

Δ

Δ

Δ

Δ

8

D17S951

1

1

1

1

1

1

1

1

1

1

1

1

1

3

SOST

C

C

C

C

C

C

C

T

JM6307

1

7

1

1

1

1

1

1

1

1

1

1

1

4

(D17S2253)

D17S2234

2

1

2

2

2

2

2

2

2

2

2

2

2

2

D17S1860

7

11

7

7

7

7

7

7

7

7

7

7

7

10

BP7129

2

6

2

2

2

2

2

2

2

2

2

2

2

4

BP7141

10

1

10

1

10

1

10

10

10

10

10

10

10

8

D17S2235

3

1

3

1

3

1

3

3

3

3

3

3

3

3

D17S1861

8

13

8

2

8

2

8

8

8

8

8

8

8

8

Example 2

Sclerosteosis and Van Buchem's Haplotypes are Distinct

To determine whether the Dutch van Buchem disease haplotype was related directly to the disease haplotype found in Afrikaner individuals affected with sclerosteosis, scierosteosis DNA samples were typed with the same set of markers described in Example 1. These were then directly compared to the van Buchem disease DNA samples. The analysis showed marked differences between the two disease haplotypes (Table 1). In instances where the two disease chromosomes carried identical alleles at adjacent markers, true identity by descent (IBD) was unlikely, due either to the fact that one or the other shared allele was found at a high frequency (>0.50) in normal unaffected controls (not shown), or to the close spacing (i.e., <50 kb) between the markers. Finally, the van Buchem disease samples were typed for a single nucleotide polymorphism (SNP) specific for the Afrikaner SOST gene mutation. It was found that they all carried the normal “C” allele. These results indicate that van Buchem disease haplotype is distinct from sclerosteosis haplotype.

Example 3

Van Buchem's Disease is Caused by a Specific Chromosomal Deletion that Represents a Unique Identifier for the Disease Chromosome

This example discloses that patients afflicted with VBD have a chromosomal deletion of approximately 52 kb that is correlative of the disease phenotype.

Characterization of all the candidate genes within the 1 Mb region between polymorphic markers BP12574 (D17S2250) and JM6307 (D17S2253) revealed a specific chromosomal aberration that segregated absolutely with Van Buchem's disease. Two independent experimental approaches to identifying the VBD candidate region revealed the presence of a chromosomal deletion associated with VBD.

First, a publicly available oligonucleotide primer pair (i.e. the SSLP designated D17S1789; see FIG.

1

A and Table 5) failed to PCR amplify a DNA fragment from genomic DNA obtained from VBD patients while permitting the amplification of a DNA fragment from genomic DNA obtained from normal individuals. As part of the present invention, the nucleotide sequence of a 92,101 bp stretch of genomic DNA from a normal individual, encompassing the D17S1789 amplified fragment, was obtained and is presented herein as SEQ ID NO:1.

Second, the 92 kb stretch of DNA was computationally analyzed, using the GENSCAN exon prediction algorithm, to identify putative exon sequences (Burge et al.,

J. Mol. Biol

. 268:78-94 (1997)). PCR primer pairs corresponding to sequences flanking each exon were designed and tested in amplification reactions using as template the genomic DNA obtained from non-VBD-afflicted individuals and individuals afflicted with VBD.

FIG. 1A

discloses the normal exon structure across the relevant chromosomal region between the Mox1 and SOST genes, as predicted by the GENSCAN exon prediction algorithm. Exons that amplified from both VBD and normal DNAs are indicated by the filled blocks (i.e. 1-4 and 26-28) while exons that failed to amplify from VBD DNA are indicated by the open blocks (i.e. 9-19). The location of the amplicons that include these exon sequences within the 92 kb nucleic acid of SEQ ID NO:1 and the respective SEQ ID NO assigned to each of the exon sequences as provided by the present invention are indicated in Table 2. The results of these amplification reactions using genomic DNA from VBD afflicted and nonafflicted individuals is summarized in Table 4.

TABLE 2

Amplicons Spanning Potential Exons Predicted by GENSCAN

Location in

Amplicon Name

Amplicon #

Primers (DMO#)

SEQ ID NO:1

SEQ ID NO

Gscn12.210A.09

19

12098/12104

14021-13436

SEQ ID NO:17

Gscn12.210A.8

18

12097/12103

18353-17837

SEQ ID NO:16

Gscn12.210A.7

17

12090/12096

18903-18334

SEQ ID NO:15

Gscn12.210A.6

16

12089/12095

19556-18884

SEQ ID NO:14

Gscn12.210A.5

15

12088/12094

19593-19063

SEQ ID NO:13

Gscn12.210A.4

14

12087/12093

29498-28949

SEQ ID NO:12

Gscn12.210A.3

13

12086/12092

33353-32666

SEQ ID NO:11

Gscn12.210A.2

12

12085/12091

35036-34373

SEQ ID NO:10

Gscn12.210A.1

11

12078/12084

36817-36212

SEQ ID NO:9

Gscn12.668.08

10

12110/12116

49386-49957

SEQ ID NO:8

Gscn12.668.07

9

12109/12115

50550-51151

SEQ ID NO:7

Gscn12.668.06

4

9260/9261

58974-58394

SEQ ID NO:6

Gscn12.668.05

3

12709/12710

58922-59740

SEQ ID NO:5

Gscn12.668.04

2

12101/12107

67161-67717

SEQ ID NO:4

Gscn12.668.03

1

12100/12106

75612-76103

SEQ ID NO:3

In total, 16 fragments, corresponding to the SSLP (D17S1789) and at least 15 potential coding exons, were successfully amplified from genomic DNA obtained from non-VBD-afflicted individuals. In contrast, 12 of these 16 fragments did not amplify from genomic DNA obtained from individuals afflicted with VBD. These results revealed the presence of, and partially localized, a genomic deletion within human chromosome 17q21 in the locus associated with VBD (see FIG.

1

A).

To better define the endpoints of this chromosomal deletion, additional PCR amplification reactions were performed with primers designed to amplify within the presumably non-coding regions located between the exons at either end of the VBD-specific deletion. The PCR primer pairs used and results obtained are summarized in Tables 3 and 4.

TABLE 3

Amplicons Corresponding to Non-coding Sequences

Located within SEQ ID NO:1

Location in

Amplicon Name

Amplicon #

Primers (DMO#)

SEQ ID NO:1

SEQ ID NO

5′moxA

25

12697/12698

1499-1018

SEQ ID NO:27

5′moxB

24

12699/12700

2524-2065

SEQ ID NO:26

5′moxC

23

12701/12702

5608-4939

SEQ ID NO:25

5′moxD

22

12703/12704

8684-8249

SEQ ID NO:24

5′moxE

21

12705/12706

11248-10880

SEQ ID NO:23

D17S1789

20

1789for/rev

14257-14060

SEQ ID NO:22

668intronD

8

12622/12623

51371-52377

SEQ ID NO:21

668intronC

7

12624/12625

53062-54232

SEQ ID NO:20

668intronB

6

12626/12627

55163-56398

SEQ ID NO:19

668intronA

5

12628/12629

57103-58202

SEQ ID NO:18

The deletion associated with VBD was further characterized by amplifying a fragment from VBD genomic DNA, using the PCR primer pair 9260/12702 (SEQ ID NO:30/SEQ ID NO:77), sub-cloning this fragment and sequencing it to completion. As disclosed in

FIG. 2

, the 9260/12702 primer pair spans the deletion identified in VBD afflicted individuals. The 2317 bp fragment obtained from PCR amplification comprised a deletion breakpoint flanked on both the 5′ and 3′ ends with normal genomic DNA sequences. Without being limited to any particular theory of the present invention, it is believed that these results indicate that the VBD aberration is a simple chromosomal deletion, with no other associated rearrangements. The nucleotide sequence spanning the deletion breakpoint is depicted in FIG.

1

B and is provided in SEQ ID NO:101.

Comparison of the SEQ ID NO:101 with the 92,149 bp sequence obtained from non-VBD-afflicted individuals revealed that approximately 52 kb of contiguous genomic DNA is invariably deleted from chromosome 17 at position 17q21 in individuals afflicted with Van Buchem's disease. This deletion is not present in individuals who are non-carriers or not afflicted with Van Buchem's disease. The sequence of the 52 kb sequence is provided in SEQ ID NO:2.

TABLE 4

Amplification Results Obtained from VBD Affected and Unaffected

Genomic DNA Samples

PCR Results

Amplicon Name

Amplicon #

Primers (DMO#)

VBD Affected

Unaffected

Mox1.03

28

12158/12164

+

+

Mox1.02

27

12157/12163

+

+

Mox1.Oi

26

12265/12156

+

+

5′moxA

25

12697/12698

+

+

5′moxB

24

12699/12700

+

+

5′moxC

23

12701/12702

+

+

5′moxD

22

12703/12704

−

+

5′moxE

21

12705/12706

−

+

D17S1789

20

1789for/rev

−

+

gscn12.210A.09

19

12098/12104

−

+

gscn12.210A.8

18

12097/12103

−

+

gscn12.210A.7

17

12090/12096

−

+

gscn12.210A.6

16

12089/12095

−

+

gscn12.210A.5

15

12088/12094

−

+

gscn12.210A.4

14

12087/12093

−

+

gscn12.210A.3

13

12086/12092

−

+

gscn12.210A.2

12

12085/12091

−

+

gscn12.210A.1

11

12078/12084

−

+

gscn12.668.08

10

12110/12116

−

+

gscn12.668.07

9

12109/12115

−

+

668intronD

8

12622/12623

−

+

668intronC

7

12624/12625

−

+

668intronB

6

12626/12627

−

+

668intronA

5

12628/12629

−

+

gscn12.668.06

4

9260/9261

+

+

gscn12.668.05

3

12709/12710

+

+

gscn12.668.04

2

12101/12107

+

+

gscn12.668.03

1

12100/12106

+

+

TABLE 5

Polynucleotide Sequences Derived from SEQ ID NO: 1

Primer

Name

Nucleotide Sequence (5′-3′)

SEQ ID NO

1789for

ATTGGCCTGGCTTCTG

SEQ ID NO:28

(5522)

1789rev

GGCTGGAGCAGGGACT

SEQ ID NO:29

(5524)

9260

TCCTGTGATCGCATTGAGAC

SEQ ID NO:30

9261

CCCTGCCATTCTGGATAGTTT

SEQ ID NO:31

12078

CAGTGGCTTTATTTTCCTAA

SEQ ID NO:32

12084

GAAGCTTCTCCATGTTCTTA

SEQ ID NO:33

12085

GTCTAAAAATGAAGAAGGCA

SEQ ID NO:34

12086

TTAAGTGACTTGTCCGAGAT

SEQ ID NO:35

12087

CAACTCAATCTTTTGGTGTT

SEQ ID NO:36

12088

AAATCAGATTCAAGCAGTGT

SEQ ID NO:37

12089

TCTTAACTGGCTTTTCAGAC

SEQ ID NO:38

12090

ATTGTTTCATTTTACCCTCA

SEQ ID NO:39

12091

GCCATAAAATCAGGATAATG

SEQ ID NO:40

12092

TGCCTAGAACATTCTGGTAT

SEQ ID NO:41

12093

CAAAGTGGCTCTGATTATTT

SEQ ID NO:42

12094

GGATCTCCTACGTACTGCTA

SEQ ID NO:43

12095

TGAGGGTAAAATGAAACAAT

SEQ ID NO:44

12096

AAAGACACTGCAGAGAAAAG

SEQ ID NO:45

12097

CTTTTCTCTGCAGTGTCTTT

SEQ ID NO:46

12098

GAGACCTCTCCTCTTTGAAT

SEQ ID NO:47

12100

GGTTTCAACTAGTTCTGGTG

SEQ ID NO:48

12101

CTAGGGCTTAGAAGTTTCCT

SEQ ID NO:49

12103

ACTCCTAGAACCCTAAAGGA

SEQ ID NO:50

12104

GTATCACCAGTGAAGTTGGT

SEQ ID NO:51

12106

GATAAATGGATATGGCAAAG

SEQ ID NO:52

12107

GGTTTATAATTTGCAACCAG

SEQ ID NO:53

12109

ATTCCCTAAGAGATTTGTCC

SEQ ID NO:54

12110

GAGAGGACAAACATTCAAAC

SEQ ID NO:55

12115

TTTAGACCTATCACTCCCAA

SEQ ID NO:56

12116

CTACTGGGACAAACCATTAC

SEQ ID NO:57

12156

AGAGAGGGTGAGTAACTTCC

SEQ ID NO:58

12157

AATAAAAGAAAGTTTGGGGT

SEQ ID NO:59

12158

GCAGAGTGCTTTTAGAACAT

SEQ ID NO:60

12163

AGGTGGAGGTTACAGTAAGA

SEQ ID NO:61

12164

AAGCAGTATCTCTGAAGCTG

SEQ ID NO:62

12265

CCTTTTCTTGGTTCAGATAA

SEQ ID NO:63

12622

ACGGTGTACACATTTGGTTAG

SEQ ID NO:64

12623

CGGATATTTGTCTGTGATACG

SEQ ID NO:65

12624

ACCGTGCAGAGGTAGATGGTA

SEQ ID NO:66

12625

CCAGTGGAAGAGACAGGTGA

SEQ ID NO:67

12626

GAGCTGAGATCGCACCACTT

SEQ ID NO:68

12627

CAACACGACATGGAATGGACT

SEQ ID NO:69

12628

CACAGCTGGAGACATGTTACA

SEQ ID NO:70

12629

AGGGTTCACACCATATCAGAA

SEQ ID NO:71

12697

ACGCTGCTGTTAAGGTCCA

SEQ ID NO:72

12698

TGCCAATTAGCCACACTCTTC

SEQ ID NO:73

12699

GTGCAAAGTGCCTTACACAG

SEQ ID NO:74

12700

GAGGTTAGACGGGTCTGAGTT

SEQ ID NO:75

12701

TGGCAGGCAGTAGTAACTCTG

SEQ ID NO:76

12702

CTGGGATTACAGGTGTCTGG

SEQ ID NO:77

12703

TGAGCTGTTCCCACACCACAT

SEQ ID NO:78

12704

TCAGGACGTTGCACTTTGACA

SEQ ID NO:79

12705

GAATGCTGGATGTGGATTGAG

SEQ ID NO:80

12706

GAGCAGAAGGCCTTGACTGA

SEQ ID NO:81

12709

GCCTTCAGTTTGTCCCGTTCT

SEQ ID NO:82

12710

CGCGAGGTCAGGAGTTCGAT

SEQ ID NO:83

12952

CCACTGAACAGGGCACAAGAT

SEQ ID NO:84

VBspan1/

GAATTACTGGCTGAGGCAACC

SEQ ID NO:85

12953

Span1F

GCCTGCGCTTACATAGT

SEQ ID NO:86

Span1R

AGGCGGGCGGATCACAAGGTC

SEQ ID NO:87

Span2F

GGCTCACTGCAACCTCCACCTA

SEQ ID NO:88

Span2R

AACCAAACAAGCCAGCAATCACTC

SEQ ID NO:89

Wt1R

TGGGAGGCTGAGGCAAGAGAAT

SEQ ID NO:90

Wt2F

ACTGGGCCTGGGATGTAT

SEQ ID NO:91

Wt2R

AAAAATTGGCTGGGTGTGG

SEQ ID NO:92

Wt3F

CAGGCTGGAGTGCAATGGTGTG

SEQ ID NO:93

Wt3R

TGGGAGGCTGAGGCAAGAGAAT

SEQ ID NO:94

Del1F

CTCACTGCAGCCTCAACTCG

SEQ ID NO:95

Del1R

TAGCAGAAGGGGGAAGGGGGAACG

SEQ ID NO:96

Del2F

GACGGTGTACACATTTGGTTAGTT

SEQ ID NO:97

Del2R

GCACAGTTACATCCGCCTTATCGT

SEQ ID NO:98

Del3F

TGCAGACACCCAGAGAAGACGACT

SEQ ID NO:99

Del3R

TGGACCCTGCCCTCACGATGGA

SEQ ID NO:100

VBspan2

TACTACTGGGCCTGGGATGTA

SEQ ID NO:104

VBint1

TAGAGAAAGACCTCGTTATTGG

SEQ ID NO:105

Example 4

First Methodology for Identification of Van Buchem's Disease Carriers by Detection of a 52 KB Genomic Deletion

This example discloses a methodology for distinguishing individuals who are afflicted with or carriers of Van Buchem's disease as well as from individuals who are normal with respect to the 52 kb deletion. The methodology is based on the observation that the large, i.e. 52 kb genomic deletion that correlates absolutely with Van Buchem's disease may be identified by amplification of the region encompassing the deletion breakpoint.

The deletion on the Van Buchem's disease chromosomal locus 17q21 results in the apposition of DNA sequences normally separated by approximately 52 kb. A PCR assay specific for the deleted chromosome was developed. Starting with the sequence of the 2.3 kb VBD-specific fragment that includes the deletion endpoints, primers were designed to span the endpoints and yield a 400-800 bp amplicon from VBD DNA but would not, because of the presence of the 52 kb genomic fragment, permit the amplification of any fragment from normal, non-carrier genomic DNA samples.

As illustrated in

FIG. 2

, the following oligonucleotide primer pairs were used to amplify chromosomal DNA within the locus 17q21 from individuals who are afflicted with or carriers of VBD: Primer Nos. 12952 and 12953 hybridize within the region upstream and downstream, respectively, of the 52 kb genomic region deleted in VBD-afflicted individuals and amplify a 798 bp fragment when the 52 kb region is deleted; Primer Nos. Wt2F and Wt2R hybridize upstream of and within, respectively, the 52 kb genomic region deleted in VBD-afflicted individuals and amplify a 473 bp fragment in the absence of the deletion; Primer Nos. 9261 and 9260 hybridize within the region downstream of the 52 kb genomic region deleted in VBD-afflicted individuals and amplify a 581 bp fragment in all cases.

The VBD diagnostic was designed such that individuals who are homozygous for the 52 kb deletion and therefore afflicted with VBD may be differentiated from individuals who are heterozygous for the 52 kb deletion and therefore carriers of VBD as well as individuals who are normal with respect to the 52 kb deletion. Two separate PCR reactions were performed on the individual to be tested. In the first, a primer pair spanning upstream and within the VBD deletion (i.e. the Wt2F/Wt2R oligonucleotide primer pair) yielded an amplicon from normal non-carriers and heterozygous carriers. In the second reaction, primers which span the 52 kb deletion (i.e. the 12952/12953 oligonucleotide primer pair) yielded an amplicon from heterozygous VBD carriers and homozygous patients. A primer pair spanning a region unaffected by the 52 kb genomic deletion (i.e. the 9260/9261 oligonucleotide primer pair) was included as a positive control for the PCR reactions.

The following reaction conditions were used for amplification of 400 to 800 bp nucleic acid fragments using as template genomic DNA isolated from patients who are afflicted with or carriers of Van Buchem's disease: 25 ul reactions included 10 ng genomic DNA; 60 mM TrisHCl, pH 7.5; 15 mM Ammonium Sulfate; 2.5 mM Magnesium Chloride; 0.4 mM each of dATP, dCTP, dGTP and dTTP; 0.2 μM each of Primer Nos. Wt2F and Wt2R, or 12952 and 12953, or 9260 and 9261. The fragments (473, 798, and 581 bp, respectively) were amplified by the following protocol: Denaturation at 94° C. for 3; 40 cycles of 94° C. for 1 minute, 68° C. for 1 minute and 72° C. for 1 minute; final extension at 72° C. for 5 minutes. The amplified nucleic acid fragments were resolved on a 1% agarose gel using 1×TAE buffer. DNA bands were visualized with ethidium bromide staining.

The results are summarized in FIG.

2

. The summary shows that nucleic acid fragments amplified in all PCR reactions containing the 9260/9261 oligonucleotide primer pair regardless of the source of genomic DNA template. The 12952/001 oligonucleotide primer pair amplified a nucleic acid fragment from non-VBD afflicted individuals and individuals who are heterozygous for and therefore carriers of VBD but did not amplify a nucleic acid fragment from individuals who are homozygous for and therefore afflicted with VBD. The 12952/12953 oligonucleotide primer pair amplified a nucleic acid fragment from individuals who are heterozygous for and therefore carriers of VBD and from individuals who are homozygous for and therefore afflicted with VBD but did not amplify a nucleic acid fragment from non-VBD afflicted individuals.

These results demonstrated that a first methodology presented herein permit the differentiation between an individual who is homozygous for the 52 kb genomic deletion and therefore afflicted with VBD, an individual who is heterozygous for the 52 kb genomic deletion and therefore a carrier of VBD and an individual who does not bear the 52 kb genomic deletion and is therefore normal with respect to VBD.

Example 5

Second Methodology for Identification of Van Buchem's Disease Carriers by Detection of a 52 KB Genomic Deletion

This example discloses a second methodology for identifying individuals who are afflicted with or carriers of Van Buchem's disease as well as from individuals who are normal with respect to the 52 kb deletion. The methodology is based on the observation that the large, i.e. 52 kb genomic deletion that correlates absolutely with Van Buchem's disease may be identified by amplification of the region encompassing the deletion breakpoint.

As illustrated in

FIG. 3

, the following oligonucleotide primer pairs were used to amplify chromosomal DNA within the locus 17q21 from individuals who are afflicted with or carriers of VBD: Primers VBspan2 and VBspan1 hybridize upstream and downstream, respectively, to a region that flanks the 52 kb genomic region deleted in VBD-afflicted individuals and amplify a 642 bp fragment when the 52 kb region is deleted. Primer VBint hybridizes within the 52 kb genomic region which is deleted in VBD-afflicted individuals and with primer VBspan2 amplify a 720 bp fragment in the absence of the deletion.

The VBD diagnostic was designed such that individuals who are homozygous for the 52 kb deletion and therefore afflicted with VBD may be differentiated from individuals who are heterozygous for the 52 kb deletion and therefore carriers of VBD as well as individuals who are normal with respect to the 52 kb deletion.

A single multiplexed PCR reaction was performed on the individual to be tested. The pair of oligonucleotide primers span the 52 kb deletion (i.e. the VBspan1/VBspan2 oligonucleotide primer pair) yielded an amplicon from heterozygous VBD carriers and homozygous patients. The second pair of oligonucleotide primers pair spanning upstream and within the VBD deletion (i.e. the VBspan2/VBint1 oligonucleotide primer pair) yielded an amplicon from normal non-carriers and heterozygous carriers. A primer pair spanning a region unaffected by the 52 kb genomic deletion (i.e. the 9260/9261 oligonucleotide primer pair) was included as a positive control for the PCR reactions.

The following reaction conditions were used for amplification of 400 to 800 bp nucleic acid fragments using as template chromosomal DNA isolated from patients who are afflicted with or carriers of Van Buchem's disease: 50 μl reactions include 20 ng genomic DNA, 0.4 μM each of Vbspan1 and Vbint1, 0.8 μM of VBspan2, 0.2 mM of each dNTP, 120 mM TrisHCl, pH 7.5, 30 mM ammonium sulfate, 5.0 mM magnesium chloride and 2.5U Taq polymerase (Roche Molecular Biochemicals, Indianopolis, Ind.). The fragments (642 and 720 bp) were amplified by the following protocol: Denaturation at 94° C. for 3 min, 40 cycles of 94° C. for 30 sec, 63° C. for 30 sec, 72° C. for 2 min and a final extension of 72° C. for 5 min. The amplified nucleic acid fragments were resolved on a 2.5% agarose gel using 1×TAE buffer. DNA bands were visualized with ethidium bromide staining.

The results are summarized in FIG.

3

. The summary shows that nucleic acid fragments amplified in all PCR reactions containing the 9260/9261 oligonucleotide primer pair regardless of the source of genomic DNA template. The VBspan2/VBint1 oligonucleotide primer pair amplified a nucleic acid fragment from non-VBD afflicted individuals and individuals who are heterozygous for and therefore carriers of VBD but did not amplify a nucleic acid fragment from individuals who are homozygous for and therefore afflicted with VBD. The VBspan2/VBspan1 oligonucleotide primer pair amplified a nucleic acid fragment from individuals who are heterozygous for and therefore carriers of VBD and from individuals who are homozygous for and therefore afflicted with VBD but did not amplify a nucleic acid fragment from non-VBD afflicted individuals.

These results demonstrated that a second methodology presented herein permit the differentiation between an individual who is homozygous for the 52 kb genomic deletion and therefore afflicted with VBD, an individual who is heterozygous for the 52 kb genomic deletion and therefore a carrier of VBD and an individual who does not bear the 52 kb genomic deletion and is therefore normal with respect to VBD in a single multiplexed amplification reaction.

In providing the forgoing description of the invention, citation has been made to several references that will aid in the understanding or practice thereof. All such references are incorporated by reference herein.

From the forgoing, it will be appreciated that, although specific embodiments of the invention have been described herein for purposes of illustration, various modifications may be made without deviating from the spirit and scope of the invention. Accordingly, the invention is not limited except as by the appended claims.

105

1

92139

DNA

Homo sapiens

misc_feature

7043, 8369, 8401

n = A,T,C or G

1
caaagatttg attctttata ctttttatgt tcaaattttt aaaaatgcaa aagaaaaaaa 60
actaaatagg agggaaaaat gttcaacaga aaatttaccc caggaaccaa aaaaaaaaaa 120
aaacccaaaa ctaaagtctc tccttaaagt acacacacat gtggccagct actcctatgt 180
gtgtgcacac acctatgtcg ggcttgctcc tgcccctcca atgcaccagc ctacctactg 240
ggatcccctg tatgtgtatt tgtcgccaat gacactaaac attttcactg tcccaaccca 300
gacgcaccag ctgacgagga gttcgtcctg gagcccagag caaatgcctg cagagggctg 360
gaccagggct gctgggacac acttttaaat cctgcggtgg ggagagagtg acaaatttct 420
gaagtgaaat gtgagagagg agagggaggg gttaacccac acacacaaga tcccctccaa 480
ccaaaaccct agcaggcaac tattaatcat cagaaacctt atatgcacat ctaatgggat 540
ttgcagatgg agacttttaa agaaacattg tctgtatttt ttttttaagg aaagagagca 600
gcacacacac acatacacac acaccgtctt taatgtgcct cctgtaacac tatgaagtta 660
tttttattgc actgttaaca aaattcccca agtcttggat ttagaaaaag ccttaagtca 720
gatccagaat ccatcaaatg ccaaattcca gggagtgaag tgaggaataa gagcaagaga 780
caagcttggt gattgcattt gatttaaagg cccttctact gctgcctgcg aaaaaggaag 840
gtggattaaa agaaatcttt tttgcaagtc tcagcggccc actgtggtct gcagtacaaa 900
gaaaggcaca ctctagaaaa aaagaatttc tctctgccca gagtgacatt ctcactttct 960
cagtaactta aaaaaacaat cagattcttc cttccgctct ttaacctcca acatacatgc 1020
caattagcca cactcttctc tagagaggtt tcaagtcatt tttcttcaca gcaatggcaa 1080
ggctcttaaa taaggtcctt gaagtttctt cgggtctcct cccacctgct ccctgccccc 1140
ttcacctcca cccacctgct tccctttctc atcccccaga ggcggaggct tccgaggaat 1200
ttggggtagg gaaataggaa tcaggggctc ctcattcccc aaaggagcct ctttggcaag 1260
caggcacgtg ggtctccggg ctggtgcaca tcacagggca gccagcccaa gtgccatctt 1320
gatgcccaat cagtcttcct catggctgcg ccctgctggt ctctcagagg gttaatgcaa 1380
tttcttggag gacgacattc ctaacaccca ggggccagaa ctccttcccc actggttatt 1440
cccatggccc agagcagcag gatgggggca gaaacaggca tggaccttaa cagcagcgtc 1500
tctgctggct gcttccaaag acggcgaggg ttccctgagg aggggcccct aggtcttagc 1560
tctgcagcct ggaggtgggg ccctgggggg tgggtgcagg agatgctcct aggaggagcc 1620
gaagggcacc cagggatgtg gaatgaagcc acgactggcc tttaatccag aaactccagt 1680
cagatacaca tcctgggagc aggagcagct gtaaaagatg gggaggggtg tacatatgaa 1740
aagatgtgcc ctcttctgga acaagcggtc agagtgttgt agaccagtag agtggatggg 1800
gtcatagctg tcaaccagac tcatctttct gctttgccca gggcttagct gtggcacaca 1860
caattctctg agcctcacaa ctttcctagg atgggtaagt aagaattatg tctccatttt 1920
gcagatgaca aaaccgaggc tcagatggat gactgggctg cccagactca cacagctaag 1980
aaatggtgga atcagcactt ggcctcaggt gtcctgctgt tcagtttgga gcaggccaga 2040
tgcagaggag gaggcatggg gtttgaggtt agacgggtct gagttcaagc cccaggtctg 2100
ccacttcctg gctgtgtgcc ctggaacaag tcaccttatc tctctgaact tctttttttt 2160
ttttttcttt ttgagatgga gtctcgttct gtcgcccagg ctggagtgca gtggcatgat 2220
ctcagctcac tgcaagctcc gcctcccggg ctcacgccat tctcttgcct cagcctcccg 2280
agtagctggg actacaagca cccgccacca cgcccggcta attttttgta ttttttagta 2340
gagacggggt ttcaccgtgt tagccaggat ggtctcaatc tcctgacctc gtgatccgcc 2400
cgcctcggcc tcccaaagtg ctgggattac aggcatgagc caccatgcct ggccatctct 2460
ctgaacttct gtttcctcat ctgagatgac agtcagagtg ggatctgtgt aaggcacttt 2520
gcaccttaca catttttacc cccacggatt cccacaaaag ccttctgagg caacagtcat 2580
tctcatcagc tccgtttctc tctctctctt ttctttttat cagctccgtt tttcagcaga 2640
agactctgat gcacagatag actgtgtaac ctgcacaagg ccacacagca gggaagcagc 2700
ggacccagga ttcaaattga ggcagcccag cccacagaca tttgcaaatc ttcaatgaaa 2760
tcctttatgt aggccaggca cggtggctca gacctataat cccagcactt tggaaggctg 2820
aggcaggcag atcatttgag gccaggagtt caagaccagc ccggccaaca tggtaaaacc 2880
ccgtctctaa tagaaataca aaaattaggc cgggcgcggt ggctcatgcc tgtaatccca 2940
ccactttggg aggccgaggc gggtggatca tgcggtcagg agatcgagac catcctggct 3000
aacacagtga aaccccgtct ctactagaaa tacaaaaaaa aaaaaaaaaa ggcgggcgtg 3060
gtggcgggcg cctgtagtcc cagctacttg ggaggctgaa gcaggagaat ggtgtgaacc 3120
cgggaagcag agcttgcagt gcaccgagat cacgtcactg cactccagcc tgggtgacag 3180
agcgagactc catctcaaaa aaaaaaaaaa aaagaaatac aaaaattagc tgggtgtggt 3240
ggcacatgcc tgtaatccca gctactcggg aggctgaggg caggagaatc gcttgagcct 3300
gggaagcaga gaggttgcgg taagccaaga ttgtacaact gcactccggc ctgggcgaca 3360
gagtgagaca ctgtctcaaa aaaaaaaaaa aagaaaaaga aaaagaaata aagaaagaaa 3420
tcctttatgc aaaaggcaga gtaagtaatg gacaaatgtg gctcccttcc tagcacatct 3480
atttctggtt aacctcgatg atcccaaagg gtgaacctgg gaatggggag ttctgaggaa 3540
attctacaga aacagccttg tgaggtcctt tgtggggggc acgctgtgct gtgggggttc 3600
tggaaggaat ccgtgggagg ctgggaggaa gatctggctt gtcagcttcc ctaggaaaac 3660
cttcccctgg gctggccgca ggctgtaacc ggattcctgc tccacctctg catctggccc 3720
agggacctca tggcagggag gcccagcgcc tggccctttg cccctggacg gggtgggccc 3780
tgggtcatgg tggggtgggt ggggaggtca ggagggccat ggggaggggg cgcggtgggg 3840
tgctttgccc tgagaacaca ggcctctggc accccggagc ccccggcagc tgctggcgtc 3900
tgtcagccac cttgcggggc gcggccgggg gcctgctggc ccctacatct tcctgacagg 3960
cccctcttct gaggccagga aaaaacaaca acagttcctc ccctcacggc aacccatttg 4020
ttagatgaag gccgggcacc agcaccttta acctcctcaa agtcagcgtt tccctgtcaa 4080
ggccccacag ggccagagac agagatggat ggaaggagct gtgtgtcgaa aaagccctgt 4140
ggcctcatga ggagagctct gttttcagga agggagggga ccccggtttc tgattgttgg 4200
ggagaataag gggaggaaga ggaaaagtgt gagtcacgag gaggtccccc aggggcgtgg 4260
gggggcccag gaggctttca gcctggccac acctgagcca tcacgtggaa cttgcggaat 4320
gtctcccatt gtgcgtggca ggcaggcgtg tacttggcag gacagggctg cttctgtttg 4380
tggccacccc acccccttgt gcttggagag gcagggtgtc agggcaaggc cctggacctg 4440
gaggcagaag acacgggttc aaggtgtgac cttgcctgtt accggctgtg tggtctcagg 4500
caaatcacac ccttctctga gcttcatttt tctcactggc aaaatggagt tgacagttcc 4560
ggcctcacct ggcggttagg aggataaatg aactgcatct gagaacagag ctggctgact 4620
ataaagggtg atgcatgtga ggaatagctt gtccttaact atgctgaacc actgggcagg 4680
acacagcaca atgtaggtga agacggctca tcccagcccc cagatacctt ctatggcaac 4740
ataacacaag ggtccaaatc atggccttgg gaattggggg cctgtgggtt caaacctcag 4800
ctctgttttt ttgttttttg ttttgagacg gagtcttagc tctgttgccc aggctggagt 4860
gcagtggtgg gatctcggct caccgcaacc tctgcctccc aggttcaagc gattctcctg 4920
cctcagcctc ccgagtagct gggattacag gtgtctggca ccatacctag ctaatttttg 4980
tatttttagt agagatgggg tcttgccatg ttggccagac tggtttccaa ctccccacct 5040
caggtgatct gcccgccctg gcctcccaaa gtgctgggat tacgggcatg agccactgtg 5100
cctgacctca gctctgttat taataagcta aatggctttg agcgacttgc cttatcactt 5160
gagcctcagt ttcctcatct gtaaaatggg gataaacttc ttccgtccgc atgaggatgc 5220
tgagagacgt gagtgaggtg gtctatgaaa gctcttgtca tagcctggca tgcaggggta 5280
acatctggat gatgaagatg atgatacctg agatttttgc cttacagaca actccagaga 5340
gccctgtgaa atatttatat gccactgaac agggcacaag atgaagccat tagcctgcgc 5400
ttacatagta gaatgtgtga atcagatgag atgcttggtc tctagtaaga ccttaaggga 5460
tggacagaag acaggcagat tttggatatg gtatacgtgg ctgtgggcta gcgtgtttac 5520
tactgggcct gggatgtatt tggaatgtac acatgtgtcc tttgcttctc agaacacttt 5580
gaggcagcag agttactact gcctgccagc ctgcctcaca gggttttttg ttttgttttg 5640
ttttcttttt ttttgagatg agtttcactc ttgttgccca ggctggagtg caatggtgtg 5700
atcttggctc actgcaacct ccacctactg ggttcaagcg attctcctgc ctcagccacc 5760
tgagtagctg ggattacagg tgcatgctac catgcccggc taatattttt tatttttatt 5820
tttatttatt tatttatttt tgagatggag tctcgctctg tcaccaggct ggagtgcagt 5880
ggtgcaatct cggctcactg caacctctgc ctcccaagtt taagtgattc tcttgcctca 5940
gcctcccaag tagctgggac tataggcacg cgccaccaca cccagccaat tttttgtact 6000
tttaatagag acgaggtttc actatattgg ctagggtggt cttgaactcc tgacctcagg 6060
tgatctgccc gcctcagcct ccaaaagtgc tggaattaca ggcatgagcc accgcgcctg 6120
gccctgacag ggctttgtaa tgctcaaata atatgatcca agggtcagag ttgagtaaac 6180
tatagaacat agtccttgca ttccacccca ggggcccaat aacgaggtct ttctctaaat 6240
ctcagaaaac actaagctaa gggaggtaag ttctgcagat gggttttctc tgtgtgtgtg 6300
tgtgtgtgtg tgtatacata tatatataca cacacacata tatatatata cacacatata 6360
tatacattta aacctttggt gctctacttg tctgctacca aaattgtagg cagatgactg 6420
aaaaatgtaa cacacagaat ggcctgcata gaaacctggc tcttggaagt catatgagtc 6480
agtttcctca actgtaaagt ggtggctcac tgtacagaca ctcagtaagc acccagcaaa 6540
tgaacacctt ccaggggttc tgtattggag tcagctgtat agaatgaaaa caaatgacaa 6600
aaggggaaac acacagagtt tattattctc ccccataaaa aaggccggag gtgacagtcc 6660
aagctgagat ggcctctccc ccaggcactg tctctctact ttgcccccct gcggcacctt 6720
gttcagctgg catgtcagca ttccacacag cacgaaggag gaaggacaaa tggactctcc 6780
ccatctctct gcccactgcc cccctcccca caacccgttt aaaaaataga tatggggggc 6840
cgggtgcggt ggctcgtgcc tgtaacccca gcactttggg aggccgaggc gggtggatca 6900
cgaggtcagg agatcgagac catcctggct aacacggtga aaccccgtct ccactaaaaa 6960
tacaaaaaat tctccaggca tggtggtggc aggcgcctgt agtctcagct actctggagg 7020
ctgaggcagg agaatagcgt ganccgggag gcgtagcttg cagcaaggcg agattgcgcc 7080
actgcactcc aagcctaggc gacagagcga gactccatct caaaaaaaaa aaaaaaaaaa 7140
aaatagacat gggggcctgg cgcggtggct cacgcccgta atcctagcac tttgggagtc 7200
cgaggtgggc ggatcacgag gtcaggggtt cgagaccagc ctggtcagcg tggtgaaaat 7260
ccatctctac taaaaatata aaaatcagct gggcgtggtg gcgcatggct gtagtcccaa 7320
ccactcagga ggctgaggca ggagaatctc ttgaacccgg gaggcggagc ttgcagcgag 7380
ccgagatcgt gccaccacac tccagcctgg gcgacagagg cgagactccg tctcaaaaaa 7440
aaaaaaaaaa tagacatagg gtcttgctat gttgcccagg ctggtcttaa actccttcct 7500
tgagtgattc tcccaccttg gcttcccaaa gtgctgggat ttacaggagt gagacacagt 7560
gcccagcctc tctacatttt tttttttttt ttttaagaca gactctcact cagtccctca 7620
ggctggagtg cagtggtgtg atctcagctc actgcaatct ccacctccca ggttcaattg 7680
attctcctgc ctcagcctcc caagtagctg ggactacacg cctggctaat ttttgtattt 7740
ttagtagaga cagggttttg tcatgttggc caggctgatc tcaaactcct gacctcaaat 7800
catccacccg ccttggcctc ccaaagtcct gggattacag gcatgagcca ttgtgcccaa 7860
ccctccctgc cctgttttaa ggagggtcct gttcaacaat tactctacag accaattgcc 7920
cgcaactata tacttggcag tgaggaactg ctggggaggc tgggagattc agcctatagg 7980
caccttggtg gccctaataa ctcttttatc ttcttcttct tccccatcag aacctgttct 8040
caaggaaaag agctaaggta ggctgccaga taaaatgctg aggtaagtct gttccagaaa 8100
atattgggac atagactaaa aaattgttgt ttgtttatct gaaactcaaa tttaactgag 8160
tgtcttcttt tccccctaaa tctggcaatc ctaatccaag gctaaactca ttttcaggac 8220
gcaaaaggct ctggccttac cttttgagtc aggacgttgc actttgacag aaggctctgg 8280
aaggaaactt taaagggagc cttccagagg gaaatgcggt gttggggtag gtctgccttt 8340
ggctatgggc tttctggctg ccggagggnc ccagggtccc ccaggaaagc cttctgtgga 8400
nggtcttttg agagagacaa agcagagggg tggaggaagg gcggctcagg tggaaggagt 8460
gaggacaaag gtgagtgccc ctgggcagga agtgctgaaa gagagaagga gggaggccac 8520
caggcctggg cctggagcca gcctgggaga ctcccagccg cccacttctc ggggcctccc 8580
ttttccagcc ccttgctttc gaggcagcag tgccattatt tggggaaacc agctaaccag 8640
ataggacagc aaaccgggga tttatgtggt gtgggaacag ctcaggtttc cctccctgtt 8700
tacccagcag tattttttaa aacagaaatc agcgtgtggg taaccgcagc tgtgagttac 8760
tagctctggc tgtgagggct ggggtggggg gagtctcttc agagccctct gtccactggc 8820
ctgggagcta ctgaaggaat gtgcctctcc ccatcccagg ccaggtggag aaggtggccc 8880
tgcggaagtt cccagatcac tgcccacctc acccttcccc tcccgacgaa ggccagcaca 8940
cctgggggag gtgtgatgat ggttcaaggt ctaaagcttt agagatcagt cagtttaggg 9000
gtcagaaccc atggagccag gcaagtaaac acaggttccc caagccagct gggagggaga 9060
cacctgggtg cctttgatgg gagaaagagg gggccaacag ctacttggca ctggccaatt 9120
ttcccttgca tgaacatggg cccagtgtaa ccaaccatat ctttccattt gtcaaaaaaa 9180
agccatattt ccagattttt tataggcaac ctgtcaactt ttaaatgttg gcaatgaatt 9240
caaaacactg ccattcaatt aaaagcagcc cagaacatgg cacgactggt tcttggaata 9300
ccaatctgca atctctgatc ttgtccaact ctctctgctc cccattttac agatcaggaa 9360
gctgaggccc agagaggcct agggacttag ccatggctgc agcagttgtt attgggaatg 9420
ccatgaggcc aggatgcctg ttatgtgttc tttgcccagc ctagcagttt ggctggcttg 9480
gctatgccag aggtctcacc atgcagttct caagtgcttc ctgagcatct ctcatttgca 9540
tagacatgac atttacaaac acatccatca tctacttgat tctggcaatg acaccgaggg 9600
agcagaactg gcattaatcc atttttttta atggtgagaa cactgaggtt tggagaggct 9660
ggtgacctgc caaagtcaca gtcctagaaa gggctgcagc cagggcctcc aattacagat 9720
caagggtttt tccaccccca aactcagtca tctcgtaaaa acgtcaggct ccttggggaa 9780
tacagcttga gatggataat caactcctcc taaaagggaa ccataaatgg atgagatact 9840
tgggtaaact gaatattctt tgtaacttaa agttaattga aaatccatag gttatcaatt 9900
ttccaagcag gagaataaaa tagaaggtat ttcaaagagc aattatagta ggcatggctt 9960
aatctttctt tgatgattct gaaggcattt caggggaatg taatgcctta gggcatcatt 10020
atgaacacca ataattactg tgtagggctg tgacgcaaat tctcatcaat cccctcctgg 10080
ctgtcggggt gttttcagag aggatcatgc agggagtgtg aacgtgtgcc tgtctgtttc 10140
ttgctgctct ctccgccttt gtcaatttca gggtgctgtt gtggaaaatg cagtgtccct 10200
ggaaggggga ggtcacttaa ccttaatgag cttctgtttc tttattaaaa tggggtaatg 10260
cagactccta tactcactgt caaggttttg tggggctcag atgtcagtgt cagagcagga 10320
agaaacttta gatcatgata tgcaccagct tcattctgcc atggaaaaat ctgaggccca 10380
gagaggttag gagcctcgtc caaggtcacc cagccaggga gagggtgcta gaacctgggt 10440
tttctgcttc ctaacctctt tcctgaatat aacactatgg aagaaaaaga tctggaggaa 10500
cggaaagatg aacacatgat tacacatgca aacaatactg gggtctctct gatcagagaa 10560
ggtgtgatct tccagcctca ggagaggggc catccaagtc ctggaggagg tgaacccctt 10620
agttcagggc tgggagtagc ctgggggcag cagagacact gctgtgaggt ttatagtttc 10680
atgactgtca gagcttttta aaatgtggta attttaagtg tgcagcctcc cagggtcttt 10740
cttcttttaa ttgaagaaat aaaccatctc ccctaaggca tgcttggcga aggagaaagg 10800
caggtgcaag gctcacagag gagagcagca gcctagaagg gctctgtgtc atggggaagt 10860
aaaacatccc agaaacagag agcagaaggc cttgactgag ccccaggaga ggcaggacac 10920
caggggtgca cacccataaa cacacacata cacatgtatg tctcctccct ggagcctgag 10980
agtccctata tacagcaggt gcatgtgggc cacacatcac acaaaattga atacaggcag 11040
gctcagagca ccagcacaca cgtatgtcct tgacaccctt agagatacta ctaagcacgt 11100
gtgtgtacct gctcacccat atggcagagc ccctggatct gggcagaaat gccaaagcag 11160
gggcaggcgc gtgtgcgcgc acacacacac acacacacac acacacacac tagcacagcc 11220
acaaaagctc aatccacatc cagcattcct aacaacacac acacagctag acacgctagg 11280
agacacatca ggacaatgtt tccactcccg ctgccataca catatgcagg tccacattca 11340
ccagtggggt agggtagagt ctcatcagtc cagactcgca gacagctgga cacagaggtg 11400
atctctgaaa cccaatgtct acacactgtg gtctttgtta cacacacaca cacacacatt 11460
gaaatgatgt cctcagcctt tggttatttt tggtttcttc tgagctggag tttcactctt 11520
gttgcctagg ctagagtgca gtggcgtgat ctcagctcac tgcaacctcc acctcccggg 11580
ttcaagtgat tctcctgcct cagcctccca agtggttggg actacaggcg cccgccacca 11640
caccctacta atttttgtat ttttagtgga aacagggttt caccatgttg gccaggctgg 11700
ccttgaactc ctgacctcag atgatccttc cgccttggcc tcccaaagtg ctgggattac 11760
aagcttgagc caccgcaccc ggccgagcct tcgggtattt tgaaagctga atgtgtggtt 11820
acattttctt ttctttttct tctttttttt ttgagacgga gtctctctct gtcaccaggc 11880
tggagtgcag tggcgcgatc ctggctcact gcaacctctg actccccggt tcaagtgatt 11940
ctcctgtctc agcctcctga gtagctagga ttacaggcat gcaccaccac gtccagctaa 12000
tttttgtatt tttagtagag ccagggtttc accatgttgg ccaggatggt ctccatctcc 12060
tcaaatcgtc atccgcccgc cttggcctcc cgaagtgctg ggattacagg cgtgagccac 12120
agtgcccggc ctacgttttc aaacagcaat agcattcgcc tcctctgtca gtttaacccc 12180
catcacaact cccacttttg gcacctaaac agttaatttc ccagttcatg ggccttcaaa 12240
gtcctgctct agtctctgga ggaactttca cctacagagg aaggtgtaag ggaaactagt 12300
tcatggattt aagtagaaac attttaggtg tagctttcac atacagagga gtgagaaaaa 12360
actgattcat ggatttagat ggaaacattg tagtatgaac ccagcggagg gtctgggagc 12420
gccttctggt ggtgagaatt agaaccgcag cactttctgc aatgtgccca ggccagaaag 12480
ctctaccttc tgataggacc cacttctgac cctagaatgg gggaactgat ggaggtgtca 12540
agccactgtg gtcccacagc tgcatgcagg cacaggggat aggaagagag ctacctacag 12600
gttactaaac cattcccttt taaaacagca ccaggcttat gtctactctg cgcttccatt 12660
ttctaggttt taagtggaag atatgtgaac acccagtggg ctggatggct gtccctgcta 12720
caagtctgtg atgtctccgt ccagtggcag agctggaagg caggtgctgt cggggctgca 12780
tctgccttgt tcaccagcat aggcctaaaa ccatggaggg ggtgctttgg cttagatccc 12840
cacttggcct gtgtgtgtaa gaggctctca ggcaccttaa tgctacatca ccaaccaaac 12900
ctcctgatga ttcttttagg ttctccgttt ccaggcagat tcacttctgt agatttattt 12960
atttattttt gagacagggt ctggctctgt cccgcaggct ggagtacagt gatgcaatct 13020
cggctcactg tagccttgac ctcaacttca agtgatcctc caacctcagc ctcccagtat 13080
gagggaccac aggtgtgcac catcacacct ggctaatttt tgtacttttt gtagagacgg 13140
ggtctcacca tgttgcccag ctggtctcaa actcctaggc tcaagcgatc caccaacctt 13200
ggcctctgaa atgctgggat tccaggtgtg aagcaccgcg cccagtccct gacttctgta 13260
gacgtttgta ttgtttacat ctactgtgtg caatgtacga gatgcagtca ggtgtctgga 13320
tggaccaggg gatctggcat cttatagact tgggtttgaa ccccaactga gccatttact 13380
ggctgggtga ctttgggtga gttcttaaac ctctctgacc cttaaggtgg taacagtatc 13440
accagtgaag ttggtgcaca cagcagggcc caaactgaca tctcacagca cccagcaggt 13500
cactgtgggc atgataggat gatgggtcac tgtgccagcc ctgaaggagt tcaagtccag 13560
ataggggaag gtggtggacc agacccagac agagattctg agtcgctgct gagactgggt 13620
gagggtagtg ggtacatggg aggacatata gcccggcagc ccagggctgg agtccacact 13680
caggttgggg cagcctggtc tgcctctcct gcaggagact tttccaggca ggcttgtccc 13740
tccagaatgc acgaatcaaa tcctctcagg atcagtctca ttttcctcgt gctgggggag 13800
caggctactc acagaagatg ttgttgcaaa tgtaagaatc acatgtcgat ccacaaactg 13860
gcattgagca gctacctagg agatcaaaga aactcttact ttgggagctc ctgccagggc 13920
tctttgggag gtctggctag ctctggagga agagaatgaa cttggggagg gcgtggaaca 13980
gatgaggacg caggcactgc cattcaaaga ggagaggtct cccggacagg gctggcctgg 14040
gcaggcccag ggagggtggg gctggagcag ggacttgaaa aagggagagg gctcaggaga 14100
ctcagaggag gaggaaagtg tgtgagcagt aggcagggtg tgtgtgtgtg tgtgtgtgtg 14160
tgtgtgtgtg tgtgcacgcg cgcgcatgca ggcctgtgta gggctgggaa agaacaaagc 14220
aaaagggtgc acaaggcatc cagaagccag ggcaatgcag aacaggaaca aagcagtttg 14280
gctcaagggg aaagttctga tggaaagcaa ggaaagagaa tgttagaagg gctgagagcc 14340
agaatgccca gtatggggta cagagggcaa cagaggggct gatgttggtg gcgaggtggg 14400
gaggagcatt tcacagggaa ctgggcagct ccaaggatgg gctggaggag ggacggtcct 14460
aaagggggtg gtgggagaca ggcagttagt gatgggggga gagaaatgct ctgagggggg 14520
ctgctgtagg cagggagggg caggtagcag gaggcagagg gttgagcctg cagaagcccg 14580
gagcagtctg tagctttcta atgcttccta gggaattctg ctgctgagta gccatgggtt 14640
caacaggtct ctaggatttg ccttgaatta ctgccctgag gccccctaag tctggggtct 14700
cataaattca gcggggaggg cacacctgtg tctgagtctg agcagcccag agcccccagt 14760
gttgtcagtg aacagctcac tgaacttgca gagcaattaa atcccagggc agaagaaaga 14820
gcaggagggc agggtggccc ccagctccct cccatcccgg gtttggggag agacacctct 14880
ggcctgagac tcctcgtggc ctctaacaag tcttgctctc tctctctttt tttttttttt 14940
ttaaattatt gtctcccgcc ctccttcctt cttcttggga atcagaaaag aaacctcaat 15000
gcctggcttt gcccctccct ggctgtggtc aagccatgtg acatttagac atttcagagc 15060
ctcctgactc ctgaggctgg agacagcatg aagccaaatg ggcttggcaa acagaagaga 15120
ggggcagtta acattctctg caatgctctc ttacttcccg tttaaacacc cctcctactc 15180
ggacacagac ccccaacccc tgtgccattg tgctcactcc caccttggag gggtcaggct 15240
gtggtgaaag gtcaaggtga aaagtcatga tgatcctatt gctgccacct ccctggggtg 15300
agcaagattc tgtggtggct ccagctctcc catggcagtc cacacagagg ctgttccagg 15360
ttctggagag gtcacttggt gtgacctggg aatctgcatc ttttctctgc caaagcagag 15420
ggcattgcag caaccacacc tgggtcccag tcctcaacaa agtccctcca gctcccgctg 15480
ctgtctgcaa ggtttggtca tctgtccctg cattggagaa gagtttgcac tgtgaatctt 15540
gcctccacca gggaaggctg gtaaaagtcc ctgccccggg ggccctcatt gctccagggt 15600
gcatctgggg gccagacctg tggaccctca cccttgagcc tcattttgtt cctcttagaa 15660
caaagccatt tctggtaaat aggtttgatg ggtttggcag cagggaaggc acagaacctt 15720
tcacgattag caggctcatg acaatttctc tttaggcaag agagaaggtt gaggaggaaa 15780
gactggcagg gcttggagag ctggaaagga agaaaggccc tacaggcctg gggatcccat 15840
ctctgaccac cgaccccagc ccccaactct catagactct gatttgatat tcttatttac 15900
caaagaagct gcatgtattt gcatatcaat tctctttccc atatcgacac gcagggaggc 15960
ttcttcgttt tggatcaacc agagatcacc aatgtctgca gggtgctcag cccctggtac 16020
cttggtcact ctaggcagcc tcctagatgt gcctcttaag ataggttttc tgaaatggga 16080
gaggaccacg ccccgccccc caccatggac tctggagtct gcggcgcaca aatcctgcga 16140
ttttacagat ggggaaacag aggcacggag aaagtctcag ctttcccacg gcccactggg 16200
accagaaccc aggtcgcagg acggggacag ccacctcctt tttctctcct tgcaaccagc 16260
ctgagctcgc tcagcgggtg ggggctgccg ctatccagag caccctgttt ctggatgcaa 16320
aataaaggcc caggcagtgt ttggccctcc tctgcccaca gactttggct ccagggcagt 16380
ctccgagaga gaagccttgg gtcccactgg tccgagctct gcgtgctgag tctagaggct 16440
gcagtctcta agccgaaact agcccaggcc tcacagccgc cttcccgccg gtctcccact 16500
gccttggcgg ggcgctggcg ccctctgctg gccaagtctc gctctgcgcg caaacgcccc 16560
agaaatcagc cggaagttcc ctggggcccc acaggagtga ggacccacct ggattcactt 16620
tgtcattttt ttttagctgc atgaccttaa gaaagctaat gaacctcaga gcactacctg 16680
tgaagtggaa ataatagcgc ctacacttca gggtcggtgt aaggattgca tagaataccg 16740
tatagaaagc acccagcaca ttgtaggtat gtttaatggt agcgattatt aataatgccc 16800
aactcacatg gtgagatgag atctgagaag cctccttata aaggtaaatg ataaccaagt 16860
cttgttgcaa ggcagctggt gtgttactaa tacatcatga ggtggtgggc cctctgggga 16920
ctgtcaggag acagcctctg ttttattagg aaataaaaca gaatctggtc tgctccagtt 16980
ttcttccttt tatttactgc attagaaaac tttcatttta tttattgcct attctggggg 17040
ttgagggtga ggggaaggca tgactccagc caggtaggat acaaaagtat ctcacccccc 17100
aggaggggtt tgaggaccgg ccctccggct tttagagaag ggcagctggc ttctgttgct 17160
aacgatttgg atttggcttt taacagaata tcaaggtgaa agtgaaagga gcttttcact 17220
ccttggagag tcaagagggt gtggggtggg acctccaggg gaatgaagag tgagcgaggg 17280
tgctgggggg atcccagacc taggaatcag atctggggaa ggggtctgca agacccctcc 17340
atgagtcaag aaaagccggt gggtggatgg tgagaaggac cagtgaagac actgtttgct 17400
ggagttgtcc cagccagtgg ctatgactga gactgtccca tggcgtgtgc ccagggtcct 17460
gccattggat gatgggacat tctttttttt tttttttttt ttgagacgga gtctcgctct 17520
gtcatccaga gctggagtgc agtggcgcaa tatcaaagtc gctgcagcct ccacctcccg 17580
ggttcaagca attctcctgc ctcagcctcc caagtagctg ggattacagg cgtgggccac 17640
cacatctggc taatttttgt atttttagta gagatggagt ttctactcca tcaggctggt 17700
ctccaacttc tggcctcaag tgatctgctg cctcggcctc ccaaagtgct gggattacag 17760
acgtgagtca ctgtgcctgg cctggatgga acattctgct ctttctccat ctgctgccta 17820
cccatgatgt cttgggactc ctagaaccct aaaggaagcc cctggacacg caggaaggtg 17880
tggagaggag ttctcatact tgcacttggg aggagggctc aggagaaaca gaggctggca 17940
acaccccctc acacactggt cctctggagg gccagtgtct acagacactg tggactgagt 18000
ccacagagag gaaagggtcc tgccttcatc agaactgctc agcaagcagt tccatcccag 18060
ggggtcctgc gaggtaaggg aggggcagct agctaggtga ggggctgaga gagtggggag 18120
gggaaagagg gaagaagaga gtgagaggga gagggaggga ctgagccgat tctcagctcc 18180
ttgaccgttt gctgagctct gtctgagtgg acagatggtc ccaagtcagg ccacaccaga 18240
gtggcctttc tgctccccta caccctgcat tcctcaacat tgctggcccc ggagagactt 18300
tccttcagag aagcaaatgg ctggggaatg gtgaaagaca ctgcagagaa aagaaagcac 18360
agcctgctgc cctgggaatt aacatgattt aggagacctg caggtcaccc cctcatgact 18420
aaaagccatc ctggaatgaa ggtctgtggc tatttctagg caaaactgtc tgataagata 18480
aaatagctca actcctgacc attaagtcgt gaaggccatg gccatcgtaa atctcatctt 18540
tccggccctc tggcctgcat gcagtgcagc ccagccagtc ggtggcagcc accttggtag 18600
gaagggccct catcctcctg gctgtgcccc aaggactggg caggcttcgg tgccaagggt 18660
agtgcgagca cttgaaagcc gccctgtatg tttattgttt tccccaggtg atccagaatt 18720
actcccgaac tctaccagct gaaatcctcc tcaactcaca tcagacaaga cggccctgcc 18780
acttacctgt cagatcactt tgggcaggta agctcatttt cctgaatctt tacttccaca 18840
ccttaaaatg tgagcaatac tatctccctg gcaaggttgt ttgtgagggt aaaatgaaac 18900
aataatcacg ggtgcatcct ggagctcttt cttacaaggc gtgcccccaa atctgtcccc 18960
tctttctgag gatgcccttc ccctattgtc tccctggcca tttcctaccc attctcaagg 19020
gccatgatct cagggagttc tcctgactca cccaggcata ttggatctcc tacgtactgc 19080
tacgactgca cacaggtgca ggaaatggct gtttgctttg cgtttgagga acttggaaag 19140
ggagacgtgg tagggaaagt agtggttagg ggcactttca gactgaagga tgtgggttgg 19200
ggaatacggg attcttggag ttgaggacgc cgcttctctc gtctctagct aatgtgagaa 19260
agacccttct ggacactaag cctgcaattc cactggtggc taccaggtgt ccgtggtgtc 19320
ctggggcggg tgtaatgaga gcgggagcct gtgaaaccaa aagcattgtt tttataaatt 19380
cagcactctt caatccctat taataaggtt agcggtgcag ttcttgcgtc tcctgccctg 19440
cctcaccttg caatcatatt cattggcatt cctttcttcc aagaacccac ctaggaggcc 19500
ttgcaggaga tatctctggt gctggctgtt cctgcagtct gaaaagccag ttaagataca 19560
aatatgtgag aggacactgc ttgaatctga tttcattttt taaaaagttt aattattcac 19620
agactttgct tctttctgac tagtaatgtg acacctgtga ctcagttcaa gatggtgtgt 19680
ggtgaccttg cagttgagaa gcactgagcg ctatagccat gccaagaagg ttgccctcct 19740
gggaccagca aaaattccat ccagggggcc atctctgtca ggcttgtcct gactatcttt 19800
ttggagaggc ggcagagtgg agtggttaaa agcatgattc tggagcccag ctacctggga 19860
gcaaagctcg tctctaccgc ttaccagctc tgagagctta ggcaagtgac ctattctctc 19920
tgtgcctctg agttttcatc tgtgaaatgg gagtaacaat agtcctgtct cacagggttg 19980
ctgggaggct tgaatgagtt aatgtccatg gggtgctgaa tcagtgcctg gcatacggtg 20040
aaggccatgt gagcagtaaa tattattatt attagaaagg ttggctgggc gtggtggctc 20100
acacctgtaa tcccagcact ttgggaggct gaggtgggca gattacgagg tcaagagtta 20160
gagaccagcc tggccaacat ggtgaaaccc catctctact aaaaatacaa aagacattag 20220
ccggtatagt ggtgcgtgct tgtagtccca gctactcagg aggctaaggc agaaggatcg 20280
cttgaacccg ggaggcagag gtttcagtga gctgagatca cgccactgca ctccagcctg 20340
ggcaacagag tgagactcca tctcaaaaaa aaaaaaaaaa agaaagaaag aaagaaaaaa 20400
agaaaaggtc ccaacctact cacttattgt tcacttgcat caaatgctag gttcagtgta 20460
ttgttcttgt aagattagaa atggagagat gggtcagaag gggttggtca aaatagacct 20520
ctcaacccaa gcagacaggg ctggcttcat ggccgggcaa ccatgcagga cccagggctc 20580
taagataaga aaggcacgta gtttaatgct ttgctgtccc atcttgatac tgtttttttt 20640
ttcttttttt tttggaaatg gagtttcact attgtcaccc aagataaagt acaatggcgt 20700
gatctcagct cactgcaatc tctgcctcct gggttcaagc aattctcctg cttcagctcc 20760
ttgagtagct gggattacag gcatgtacca ccacactcag ctaatttttg tatttttagt 20820
agagacgggg tttcaccatg ttggccaggc tggctggtct cgaactcctg atcgcaggtg 20880
atccacccac ctcggcccca caaagtgctg ggattacagg catgaaccac cgtgcctagc 20940
tgatattctt aatttatgaa ggaagggccc caaattttca ttttgcactg ggcccacaaa 21000
taacgtagca ggtcccacaa acaaattcgt ctagattcag agggcccctt gccttcctcc 21060
tctgctcaca ttcgttcctt tctcccatca caggcgggta ccctaccttg ggggatttgc 21120
cccagaataa gccttttttt tcccttctaa cattttaatg aaaaatttca agtgtatagc 21180
aatgttgaaa agattttata gtgagcaccc atacacctgc cacccaaagt ctaccattaa 21240
tgtcttctgg acttactttt gccgtcttat tcatactctg cctggactgt cctcagctac 21300
aggactcaca tctcttgccg acagctctaa ggcttccagt cctgctgtct ggaccaagaa 21360
aggcttcctg ggctctgagt gtcaaatggc ggccttcaag gaaggggaat ggtggaaaag 21420
gccgtggggg gttttggaga aattgctagg gaaagactgg caccagagtt ccaccagccc 21480
aggcaatggg gggtacagaa cccataagat gagttctaga aaagcaagga aggtttcgtt 21540
ctggagtttg tggactgagg tttccatttg tgactaggat tctcattggt tccttatgta 21600
gtttcttacg ccctgcatag tcttctaagc atttctcaca gacgtggtct gggggcgaac 21660
accagcagcc ctgagaggtg gctaaagagg agattcttct ctccacttca catcgtagga 21720
aactgagtct cagaggttcc ttccctggcc tgcccacaac cccagggcta aaagaggcag 21780
accaagccca gggccttgaa ccccaacaat gggcctcttt cttttgatcc catgataggg 21840
gtgcaaaagc attgcattcc cctgggtaat ttgaagaaaa aacccaaaaa actccaactt 21900
tgtctccagg aaaaagaggg tgtctgggct atgatttacc tctgagggtg tggttgcact 21960
gagcgtgatc acacttcaaa gggttagatc tcatttctct gcctttctag cttgggccca 22020
gggctcagaa atgtgtggac tccctcacag cccctcccag catccctgcc ccctcccaac 22080
tgccttgggc aggtgacacc tgtattattg ctaagggtta aaaagccccc aaatcaataa 22140
aacccattaa tgagtgttgg tacctcgaag gctacagata aatcccttct actcagtgag 22200
ttcaatccca taaaacagct ctcccctttc aatcctagca ttcatttgat agaaaatgtg 22260
gagaaatttt aaaaaggtga cttactaatt gcctgtaaaa taaaaggcag atggaagctt 22320
tattacagtt gaaggaagtc gggaatatta aggtaaaatg tcaaataaca attgattttc 22380
cttagacata aaggggcgat ttatggcttc ctagttacta caaacgagaa attatttgaa 22440
gttctgaaaa gtatgaggag aaataaagat taaatagaag atgaaatcat agggatttct 22500
ctgggaggtg acttcagtgc ccctggggac tagaattcat gtggccagtg gcctagccag 22560
ctggggcttg gcagtttcaa gatttagagg caaggtgtct ctgaggagcg gggaagtggc 22620
tgtttgcttt gtgtctgagg aactaggaaa gaaagatgaa gatagggaaa gttgtggtta 22680
gggccaattt cagactgagg gatgtgggat taggggattc ttgtggatgg gcctatagct 22740
ctgcttcctg actagcagat actggggatc tggggaaggg aagggtgagc tgcctttcct 22800
gggacttcga tggcatcctt acagtcaggg gacactgtcc ttgctgggtc ccggacatca 22860
gtgtctatgt atccctgcaa gccacagcac tatccaggcc ctctggtggc tttgcttggt 22920
ctgggccaac ctggtctcca cactgacagt caaagacgag gagaggaaaa agaactcacg 22980
gctaggcgtg gtggctcctg cctgtaattc cagcactttg agaggccgag gcgggtggat 23040
cccttgagcc caggagtttg agaccagcct gggcaacatg gcgaaatccc cgtctctaca 23100
aaaaatagag aacaattacc caggaatggt ggtactctgg aggttgaggt gggaagatgg 23160
cttgagctcg ggaggttgag gctacagtga gctgtgattg tgccaccgca ctccagcatg 23220
ggcgacagag caagacactg tctcaaaaaa aaggctcatt tccaggctgc caggcttatg 23280
ttagcctggg gtcccgcagg agctgacctg agacaagtac ttgagggcaa gttgtttgtt 23340
tgagggatgc tcacaggaag ctccagtagg agggtaggga ggtgacccag ggaaggaagg 23400
cagcttacag gggcgtgttg tcaggaaggg caccccggcg ggtgacggaa gcttaatttt 23460
gctgggaaac tcaggagcca gcatggaacc tgcacctcag agttatccca ctggaaggat 23520
gagggagcgg gtacttatac accaactcca tccccgtcct ttgttaaggc tgctggatgg 23580
agtgggtggg acactcattc ttcagcactc ccaggggccc tcaggctgtc agaagttagg 23640
tccgttgggc tccaggatga ggggaccccc agaagatgcg ggagggcgtc cgcagcatct 23700
gcctctgtgc tttccctgtt tatctgaatc ctcataattc ccgccacatg cagatcagag 23760
cccccagctt tatggaagag aacacaggtt tggagaagat aaagagcatt cccacaacta 23820
ggaggtgggg aaagctagga gttcagccca gagctccctg acttcaaagt ccattctctt 23880
tctacttcct gatttttttt tttttttttt ttttttgaga cggagtttca ctctgttgtc 23940
taggctgggg tgcagtggca caaacttggc tcactgcagt ctctgcctcc tgggttcaag 24000
caattctcct gcctcagcct ctcgagtagc tgggactaca ggcgcccgct accatgcctg 24060
gctaattttt tgtgttttta atagagacag ggtttcaccg tgttggccag gctggtctcg 24120
aactcctgac ctcaggtgat ccgcttgcct tggcctccca aagtgctggg attacaagca 24180
cgagccactg tgctcggcct acttcctgat ttttgtataa gacacatccc agcagcatgg 24240
tagactgaag actcctgggc ccactctcag agattctgct ctggcaagga tgtgtttatt 24300
ggtgagaggg gtgtccagga atacgtgcct tttttttttt ttctagagac agggtctcgt 24360
tgtccaggct ggagtgcagt ggtgcaatca tagcttactg caatctcaaa cttctgggtt 24420
cgagcgatcc tcctatctca gcctcctaag tagctgggac tacaggtgca cgccaccatg 24480
tctggctaat ttttaaattt tttctggagt tggggtcttg tgatgttgcc caggctgatc 24540
tttaacttca ggtctcaaga gatcctccca ccttggcctc ccaaattttt gggattacag 24600
ccatgagcca ctatgcccaa ctagaatatg cacttttttt tttttgagac cgagttttgc 24660
tcttgttgcc caggctggag tataatggtg cgatctcggc tcaccgcaac ctccccctcc 24720
caggtccaag cgattcttct gcctcagcct cctgaggagc tgggattaca ggcatgtgcc 24780
accataccag gctaattttg tatttttagt agagatgggg tttcttcgtg ttggtcaggc 24840
tggtcttgaa ctcctgacct caggtgatca gccccgcctg ctttggcctc ccaaagtgct 24900
aggattacag gcatgagcca ccaagcctga cctttttttt tttttttgag acagagtctt 24960
gctttgtcac ccaggctgga gtgcagtggc acgatctcgg ctcactgcaa gctctgcctc 25020
ccaggttcat gccattctcc tgcctcaggc tcccaagtag ctgggactac aggcacttgc 25080
caccacgccc ggctaatttt ttgtattttt agtagagatg gggtttcacc gtgttagcca 25140
ggatggtctt gatttcctga cctcgtgatc cacctgcctt ggcctcccaa agtgctggga 25200
ttataggcgt gagccaccgt gcccggcctt tttttttttt tttttttttt ttgagacagg 25260
atcttgcttg gttgcccagg ctggagtgca gtggcctgat tacagctcac tgcagcctca 25320
atctcctgga ctcaagcaat cctctcacct cagcctcttg agtagctggg accacaggtg 25380
tgtgctacca cacccagata atttttgtgc tttttgtaga gacagggttt cgacatgttg 25440
cccaggccgg actaaaattc ctgggctcaa gtgatcctcc tgcctcagct tcccaaagtg 25500
ctgggattac aggtatgtgc catcgtcacc agccagaata tgcattttct ttttttcaga 25560
cggggtctca ctctgttgcc caggctggag tgcagtggtg tgatctcagc tcactgcaac 25620
ctctgccttc tgagttcaag cgattctcct gcctcagcct cccaagcagc taggattaca 25680
ggtgtctgcc acaacacccg gctaattttt gcatttttag taaagatggg gtttcaccat 25740
gttggtcagg ctgatcacaa actcccgact gcaagtgatc cgcttacctc ggcctcccaa 25800
agtgttagga ttacaggcat gagccattgt gcctggccga atatgcactt ttaataagca 25860
tcagccaggc taggcaggcc gggggccaca ctcgagaaca tttgcaccac agccactggc 25920
tacctgcccc tttttccata aggttccact gccctctctc ccctctatct gggtctgttc 25980
ttcaggttct tccctgggag ctctctgagt gacataactg tccccaagtg ctgggagatg 26040
gagagaggaa tcaccagact ggagcaggcc cccagagcgg agatgggaag gggaggctgg 26100
tgttctgagg ctcccgaggc agtgagaggt gaccggaggc agtgagaggt gaccgaagac 26160
agtggctgag aaccagggag gggctgcggg aaaaagccct gggtgcaagt cgctctttcc 26220
ttagcgtctt tgagaggagg gatggggaaa ggtgaggtac tagggaaaac catctggaag 26280
gaggtcaggc tgcagaaaag ctgcaggagt ctgggggact aagaaaacag tgggagaccc 26340
cgctgcagcc cagcacgtga gggtgagaac gtcatgaatg agggaagagc aggcaggggg 26400
tggtggcctt gtggcctctg gagaggagga gccacatgac tctggggtga tctggggtga 26460
ccctcagggt aagggtacct ccctagcact gagtacagag ggaagcctac actgccaggt 26520
gcagtttccc tggcaatgct tctccttcta acactatgtg agtttcctag tgctgctgta 26580
acaaactgcc ataaaccaag tggcttcaga gaacacagac ggactatgac agttgtggag 26640
ggaagaagtt tgaaaagcgg ggtgcccgca ggctgcagtg cttctgcagg ctccagggag 26700
aatctggtcc ttgcctcttc cagcctctag agcctgccgc actccttggc tcatggcccc 26760
gtgtcattgt agcctctgct tctgccatca catctcctcc tctgcctctc ctgcctccct 26820
ctgtccttta taagaacgct tttgagtata ttggacccat ctggatagtc caggataaat 26880
ccctccatcc tcatatcctt aatttaatca cacctggaag gttccctttg ccacataagg 26940
taacatattc acattgggac agttagggga ttgggatgtg gacatctttt gaggtgggaa 27000
gaaggagctg gggttctttt taacattttt ttgagacagg gtctctgtca cctaggctgg 27060
agtgcagtgg tgtgattgag actcactgca gcagcctcaa cctcctgggc tcaagtgatc 27120
ctcccacctc agcttcctga gtagctggga ctacaggtgt gcaccaccat gcccagatga 27180
tttttgtatt attttaatat tttgtacaca tggaggtctt ttaatgctgc ccaggctgga 27240
ctcaaactcc tgggctcaag caatcctccc gtcttagcct cccaaagtgc tgggattaca 27300
ggtgtgtgcc accagaccca gcccggacct ggggttcctg tctcatgtca gccttagcaa 27360
tctgggtgac cctgggcaag gcctatcccc tctctgggcc tttgtttctc cacctgtgca 27420
atgaggctgt tccctctggc tccttcactc tgagtttttc agttgggaga atatcttggc 27480
agggagcaga ggtcggcggg ggtggttgtc attccatttc agggcctctc agagtcctgc 27540
cgtggtgtgc actgtgtgtg tgtttaattt tctacatttg gatgtgatcc taatccaata 27600
aatgcttagg agacttctat agaatagatt aatttttact agaaaaaaat ataattggct 27660
gatgttaagg ctactgccct gacaaatctg ccttggccat atatctgaga aggtaaaaga 27720
cccgctacgc ttgcacataa atatgccatc ttccccacag gccctggaga agcaccccgg 27780
ggaggtttcc cttggtgatt tattcttcat taataagctc tatgctatat taggatcaga 27840
tttatgactc tgcctttcta atatttctga catttcatct gaaaagaatt acaaatgaaa 27900
tcttgaaact ttgccacttc tccctgctag tgctctggca ctctgtgtcc aaggggagat 27960
ggtgggctgg ggagacccca agaagcaggg acagaggcat gtttctcagg gaaaggagcg 28020
atcagcttga ctttgggaga gctttattca gtttgcaagc agcttgggag gtgagcggtt 28080
caggcgagaa ggctgcagac cagaccacaa gcccagcagc agcagtgatg cctgtaacat 28140
gtatgagatg gtggcaggca cattcattca ttcaacagct atttgttaag cactttactg 28200
tgtgccaaac actgtgctgc ttggtgcttg ggatacagga aagaataaaa gtgaagcagt 28260
gatgaagatc ttggccttca tgcatatggt gttctagcag aggtagggga gtgtcaagca 28320
ctgatgaatg aaaatcctga ataggtaaaa tatatcatat gttcacacgt ggtaagggct 28380
atggcaaaac agaaacaaac cctccacaca gggaaaaggg aaccaagagt gccgggtggt 28440
caagttgcaa tgttaattaa gaaagcactt caactcatca actcaaggaa ccgtattatc 28500
cccattttac agatgaggac ggaggctcag cgtggattag gagctacaca aagacacaaa 28560
gggaactttg cacaacttga aagtttgcaa agtgccgtct gagccattag tctcccttct 28620
ccccactgac tgccctacca caatcacatg gggaccataa aaatactgtt gcctgggtcc 28680
cgctcccctg aggctttgat tcaattggtt tggatgcagc ctgggacttg gcatgtgact 28740
cttgtggtgc caggactaag aatcttgtgt gaactcccag cacagcctct caggcctcta 28800
ttttctcacc tgcaagatgt gggtaaaaat attcaccctg tactgcaaga ggatgtcagg 28860
agtgaaggca gaaacacagc accagccctc caggaccccc aacccctccc ccaccccaat 28920
ccttcacccc tgttgtacct tctgacctca aagtggctct gattatttca ctcccacagg 28980
ccactggctc agaggtatag agctcacctg tggcagatgg agatgcggat ctgaggcttc 29040
tgatgctgcc acacccagcg gcgcccccca aattccgggc ccctggatga catctggtct 29100
gttcctgcag catcagagca caatagagcc agccaccagt cccagccctg cctgcatccc 29160
atccattcct gggtgcctaa ccccgaggat cccctggcag tatgatgcgg acctgtcttg 29220
gatcccaggg atatgctggc cacggggagg agccggaaac caacctttgt gtcactgtgt 29280
agtgacaagt gcctttggag gtcacaatag ccagtggtga tttctaccac tgcccccagc 29340
agccaaggtg gcagaggagc cctgtcagtc acccccattc tgttcatggt ctcacggtgg 29400
gctccacatg gggggtggca gccctctccc ccaccccacc cgaccccttt cgacagatag 29460
ggtaatacaa atacaaataa caccaaaaga ttgagttgct gggcagaaag ggaccaaagg 29520
ccagtgtgtg tgtgaggggt gggggcaggg caggagagga gcagcaaaag gctgtgaccg 29580
cctggctgag cactggatac tcactgaagg gcagggaggc ttcctggaga aggagacctg 29640
gcaggggctg agggagtgat gccaggcatg ggggtttgga gggaccccag gcatggcatg 29700
cctccattcc tccctgtgct atccactcta tataaggggt gctgtgcagg gagacagctt 29760
gcatccaagc agggaggcag ggaggatgag aggcagagag gagcccagct gggttgatgg 29820
aaagtctggg aaatgcagga aatccaggag ggggagaatg attccaagct gtggcctgtg 29880
atgggccttg aaaccaggtg taggcacttg gatctgatcg ctggggagcc agagctgctt 29940
cctgagcagc agaagggcag gatgcgaatc agactagggg cagtgggagg aactgagagg 30000
cctcaggtca ccggagaaaa tgcacagggc cgggaggcag agatgctcct gttttcttgc 30060
tctggggctc aggacagtca gtcaccctga gcttcagact cagctcactc attttgcaga 30120
gatcctgacg gcgatgcttc aggatgatct gggaagagtc aatgaggtaa aatatgtgaa 30180
atatgccttg aaaactacaa accacagcac atgttctgtt tttgcttctg cttgatggac 30240
tcagtgagat ggtggggaca agaattagag aagcccatga ggaggccaag gggcaccaaa 30300
tagacccacc aaggaccagt ggggacttag agaaaggatg agtcagagag aaatgacagg 30360
agcagaaggc aggccttgta tggaggatga aggtgaagat catacaccat taaacttgag 30420
aaagaggcgg gaggagctgc catttcttga gtctactgga tgccagcagt agtgctgggc 30480
aagggcttaa caggtgggga aatcgaggca cagagaggtt aagtagcttg cctaagatca 30540
cccagttagt aagtagcaga gcctggcagc ttaactccaa agtctttgta ctaaagccag 30600
attttccaaa tttgcccaac tgtaagaatc acctgggcct gtaatcctag cattttggga 30660
ggctgaggtg ggtggatcac ctgaggtcag gagtttgaga ccagcctggc caacatggtg 30720
aaaccctgtc tctactaaaa atacaaacaa ttagccgggc gtggtggcag gtgcctgtaa 30780
tcctgtaatc ccagctactt gggaggctga ggcaggagaa ttgcttgaac cccaaaggtg 30840
gaggttgcag tgagctgaga ttgcgccatc gcactccagc ctgggcaaca agagagaaac 30900
gccatctcaa aaaaataaaa gcccgggagt ttattacaga tgcatattcc caggcacctc 30960
ctacggaggt tttgagttag tgagtccaag gccttctgcc tcttcccaat gtattcatta 31020
tgcaccatca ttactcttgt tcagatacaa tgtgagtgat agcttgtctc tggcagcaca 31080
gcagccacac ccaaccaatc cagacctcag tcatgagggt gccaatcaca gctaacattt 31140
ttttttttga gacggagtct tgctctgtcg cccaggctgg agtgcagtgg cacgatcttg 31200
gttcactgca gcctctgcct cctgggttca agcgattctc ctgcctcagc ctcctaagta 31260
gctgggatta caggcacctg ccaccatgcc cggctaattg tatttttagt agagatgggg 31320
gtttcactat gttggccagg ctggccttga actcctgacc ttgtgagcca cctgcctcag 31380
cttcccaaag tgctgggatt acaggtgtga gccaccgcac ctggcccaca tagctaacat 31440
ttaatcagca catacagggc catgctcatc attttttgcg cacaatgcca tttaaccttc 31500
acaacagcct gtgagaaggt gtgttagcct cattttacag aggaagaaac taaggcccag 31560
agaagttatg caacttgccc aaggacacac agcttgaagg agctgaggtt taacccgttt 31620
ctacggggtc tgaatcctcc ttaccacccc tatctcccct gactcccagg ttgtgtttgg 31680
tgtacttggg tagtgtccag ctgacaaatg agatgtttta gcttcagaca gtctatgcca 31740
tgtagaaatg cacccaggac tgtgcatatt agggaggttt gcaaatgtgt ccacattaga 31800
taatttttct caaaatgccc ctcactgaac ttctgtcatg cagtatgtac tgagcacctg 31860
cctcttgcta ggtcccggga gagaggatga agagggcaga gcccccaccc tcaggggacc 31920
tgaaaaatgg gagtcctttg tgctcatgga agcagggcct tggggcctca gcactatgga 31980
catttggggc cgggtaattc tttgttgtgg ggggctgtcc tgtagattgc aggatgctcc 32040
gccgcatccc ttgcccctgc cctctagaag ccagtagcac cctccagttg tgaaagccgt 32100
aaaatgtctc cagacattgc tcccagttga gagccacttc attaaagaaa aaaaaataaa 32160
aaagatttct gtatcctcta tacatggact gaatttcact gacatttctc taaaataact 32220
gaatctattt ctatactcat ttttttttac cagctgccat tttatttatt tttcttttct 32280
ttcttttttt aagaaacagg gtctcactct gtcacccagg ctggagtgca gtggcgcaat 32340
tatggctcac tgcagcctcg acctcctggg ctcaagcaat cctcccacct tagcgtcccc 32400
agtagctggc acgccactgt gcccggctat ttattttatt ttattttatt ttttttgtgg 32460
agacagggtc tcccaatgtt gttcaggctg gtctcaaact cctgggttta agcaattctt 32520
ctgccttcat ttcccagagt gttaggattg caggcgtgag ccactgcacc cggccatttt 32580
atttcttaac aaagcacaaa tcaaatgtac aatgcagcta gattttcctt tataaataat 32640
gtctaatgga tttgtttctg tcagctgcct agaacattct ggtatcccag acagaagtgc 32700
aacaccaggt ggaggtgttg cagctgagaa gttctgacca gcataccagc acccccttaa 32760
aagctgtcta tacttgcggt tctcagctag gctgccacag aatcgcccaa gtcttaaaaa 32820
aacaggtctc tgtcctactg cttgagatgc tggttctcta ggtctgaggt tctggcacct 32880
gtgtgttttt aaaactttac aaataattta aaagcgcctt caagtccaca acaacagaat 32940
atttccctcc actcctcatt gtcctggagt tctctaacag tgcagttcta caactggaca 33000
cacgatgtcg cttttcagcc acagttctca ctaagcggcc ccacagggcg gcaggtgcct 33060
tctgcagaga gagagagagg gccttggctg acaggccaag accgggcatc ctggctcctc 33120
ctctgtacag acttttcaca gatgtgaact ctccctactc cctgtcttct gcccccaaat 33180
gaagcctctc agctggcaag agctgagaac taccaagcga gccattgcta atttctattg 33240
tgtttggaac cacaaaaggc agaattatta aggctgtaaa ggacctcaga gcatctggtg 33300
cagtgagttt ccaacttgtg aaaatctgat gtgatctcgg acaagtcact taaccccctg 33360
cctcaatttc ttcatctgta aaataggata ggaatatatc ttgcccgtct gattgttatg 33420
aagacaaaga gaacaaatgc acataaagcc cgtgaccatg tgctttgtga atggaagctt 33480
taatttattc attcatttat tttattattt atttttattt atttatttat ttttgagaca 33540
tagtctcgct ctgttgccca ggctggagtg caatggtgcg acctcggctc actgcaacct 33600
ccatctcccg gattcaaaca attctcctgc ctcagccttc tgagtagctg ggattacagg 33660
tgcctgtcac catggccagc tagtttttgt atttttagta gagactgggt ttcaccatgt 33720
tggccagggt ggtctcaaac tcctgacctc gtgatctgcc cgctttggcc tcccaaagga 33780
agcttttatt tttattatag ttttacataa ggataaattc agccttagtg aagggaagtg 33840
acttgcccaa gatcatacag tgagactgct ggatctgggg ctctacttca gaattttttt 33900
ttgagatgga gtttcactct gttgcccagg ctggagtgca gtggcacaat ctcggctcac 33960
tgcaacctcc acctcccggg ttcaagcgat tctcctgcct cagcatccct gatagctggg 34020
actacaggct cccaccacca cgcccagcta atttttatat tttgagtaga gacagggttt 34080
caccatgttg gccaggctgg tctcaacctg cctggcctcc cagagtgctg agattacagg 34140
tgtgagccac cacgccagtc tactgccatt gtccatgatt tttcctacag ggaaaatcac 34200
aatcccagaa gataagacaa agaacagtaa aaggtggctt ttgaggcagt gagttctacc 34260
tgaaggtggg aacagcccag agtgtctggg gacagagtgg taaattctaa tcaagccttc 34320
ccatggcttt gtggatgagg atgagtttct accctgaagc tcggcctgtt cagccataaa 34380
atcaggataa tggtggctgt gcctccttag agtagaatga gaatcagagc agaacaaggg 34440
aaagctgcag tgacttgtca ggtgtcaacc ttcagcatga tgggagagcc atgggaccct 34500
tcccctttcc ctaagagagc cagccctcac agcaggcctg ggatccaatg cccagcaccc 34560
agctgggagc ccagggacct tggccaaaat ctggttctgc ttcaacttgg tgcccggcct 34620
ttgtcaagtc acttcctcat ttgcaaactg ggagagtttg gatgaaacta ttgaatgaaa 34680
ttattttggg ggtttctttc tggctctcac agtcctcgca tgctcaccat gttcccttca 34740
atttcattag cacagcccaa caaagggtta agcagtggca gttcctctcg ttctttggtt 34800
aggacaggaa ggtcaggggt gaggccaata caagaggtag ccgccacagc tgatgctgga 34860
aatgacaata gttctttcct agactcatat ttgtcccctc tccctgaagc tttgcctgca 34920
gtgcccttgt aaagaagttg gcaagaagca ggagtgaggc tcagcccctc tctgaaatgg 34980
atacgccggt tgctccccct catggctggt ctcatttgcc ttcttcattt ttagacacat 35040
tccaaacttt tcagcaaatt atagtgtttg ccaactggcc gtctggggcc caggagagat 35100
gctatttata gcgatgctgg gatgctgcca tcccagagca gcctggtaag aaacggagcc 35160
agagtgcctg ggagtggcgt cctgcaccct ggggagaggc cagggccctg gagcagggtg 35220
gcaaagctgg tggcccgtgg caaggaccac tggcacatcc cctgcctgcc tgggccctgg 35280
ggtctgtgcc cataccccac acggggggct gcttccgtgc tccttggaga gacgatggtg 35340
ctgtggggcc actgagcaca gtaaaggcta agacccacca taggtcagcc cttgctcatg 35400
ctgactgttg ccccatttcc cttcattctc tcactcgttc attcctcaga atctgcatcc 35460
tggttttgtc actacctgga gttgtaaaga taccaccaag ctcaccttgt ggtgtgagcc 35520
ttgattttcc ccatctgtgt aaagggtgga tctgagctcc aaagttcctt ctagtcatat 35580
gcagagtgca taataaatgt gtttgtattc ccactgtgct cagtgggcac tggggcatgc 35640
agaagagaaa ttggaataaa tgtgaccctt gtcctccaga ggctcagtac cagactggaa 35700
ccaggaccca gatgaggggc ctacccagag agggcagcgt gctctcctat ttgtgttagg 35760
cgttaccatt tacaaagggc tttacggctt tgaaggtccc cacaacgccc tgaggaggtg 35820
aagtgtggca atgcccgttc acttgggaaa atactgcatc tcagagagac caagggactc 35880
gcttaaggtc acacagatac agtaagtaag tagggaaaga gctggggcct ctgggctcct 35940
tttccggggc tctggcctcg gttttaaccc gttctcctag acctctcagt ctctgggcct 36000
cccctctgtg ttccccaccc ccactttcca tcaagagctc agttctctta agttctatat 36060
tctctcttcc ccacccctag aaatctctgc ccgcttccag aaaaggcttt gattccgtca 36120
tttttggcat ttcccaccca ggaatcaagg ctgcctcctc tgtgaggaag gcgtgtggaa 36180
ggcgagcagc tgaggacacc tctttttaag agaagcttct ccatgttctt agccaggtta 36240
tataacttct tttgcttctt tcttttccac attctacata tttttcacaa tgaggatgtg 36300
tcacttctat agttaaaaat ggaaatcttc atttaaaaga acaatcagac acaaggcaag 36360
gtatgcttac cttcccaatg accaaggagc agggagacat tggtcgtgga ggccataggt 36420
gaccagcctt gagggaaggg aaggaagggg aggtgcaggg aggcgcagca gaggcacccg 36480
agtgtgctct acgaatgtaa gtctgccagc tgctcctctg tgcctagcac cctggaaagc 36540
gcccgcacac agtgggccct cagcaaactc ccactgagca aagggccctg tgagtaagga 36600
cctagaagca gggtgtgctt gaggcatggg gtgggagggc atccagcttg gctggcggtg 36660
ggagccgatg caaaggtgag ctgagaccag acggtagaag accttcagtg ctgggccgag 36720
gagggttcct tcatcccata gagaagagct gccatccaag acagaaggct ggggagtgac 36780
atgttgaaat cagtatctta ggaaaataaa gccactggct gggccactgg ctcccctttg 36840
tagaggggtc tacgaagctc ccgaggtttc agatagtccc tgagagccct tcccctcgcg 36900
atgggctccg tttgaggcat gtccagtgtg aaaggaccac aggccacctg agggacagga 36960
ccaagcagag agctagtgac agaatgccca gggctccaag acagggctgg agaggtggga 37020
gtggtcccca ccttgagagc caaaggggct gagggctgag ggttgaaggc cgagagccag 37080
gaaggtccag gggagcaaaa gggggagcag agggggagct ggaggtgttg gggtggtggg 37140
tagagagcca agatagggag gggacaagag aggggagaat ctcaaggaaa agcaggagca 37200
tgagactgag agaaggcccc tggaacactg gcttgaatgt ggctgacatt ggcactgcca 37260
gtactcatcc caaaccaggg agctcagcca gcgccctttc tgcatgtccg ctctttcatc 37320
agacagctag gaacaagtta tacgagatga tgtccaagtc caataaatgt ctaagtcctt 37380
tccactggat ctggccccag cctcctctct gaaccatctc ctatcactcc accctatccc 37440
tgctggctcc ttctgctcca gccatacagg cttgctcctg ggctcagctt gtgcctgcct 37500
ggggagcctc gcgcttgctg tttcctcctt gagaaattcc tgccctggat aactccttcc 37560
cccacttcct tcagatctct gctcccttat cagggggctt cccctggctg ccctatctat 37620
aacagccctg accactcttt tcctcttctc ctgctttctt tgtcttcctg ttcttatcac 37680
taagacatca cacatgtctt agttggcttg tttatattct gtctccaccc atcttagtca 37740
gctactgctg aaatgatgct gtgtaacaaa ccaccccgat actcagaggc tccaaacaag 37800
tgctgatctt tctcatttgt gagtctgtgt gtcagcccga gcagggcagc tccaggctat 37860
ggcttgggtt tgggtggact ccatgtctct ctccattctc cttggaccag cagctccctg 37920
gggcacatgc ttgtcttgga gaatggcagg aacttaaaag ccaagccaaa cctcacagca 37980
cggttaaggg tgatcatgac acaccgctaa cattctagtg gccaaagaag tctcatggcc 38040
aagtgtaata gcagtggatt gggcaagttt actctcttac acctgttgtg aaggggagtg 38100
ggaggggatg ggggggtgac cgttttcaca acaacagtgc aaactatccc atctcccaat 38160
agaacataaa caccttaagg ttggaactgg gtctgtatcc ccagcactta aaatagtttt 38220
tggcatacag taagggtgca atagacattt gttaaataaa tatacagact aaccaattag 38280
cataatacag atgacaaagg tgtctctctt ctgccagctt ctgtgcctga aatgttgatg 38340
atctcctccc tatcttccta acatctctcc tgtccccacc tgtccatgcc cagggtttgg 38400
ctgctgtgac agaagtgtga gagcctcctg tctcccttgg gatcccagta agagcttcca 38460
tgcctctctc cctgctcacc tgggctccca tccctgggga ccttctggaa acagcttcca 38520
gggctcccag agcttactta gccagattct acatctggct ccagctgtta tcctaagctt 38580
ggccttgttt tctgatctga ccacagcttc atcactccta cctgactgtg ggattctagc 38640
ccccagtggg gtgggggacc aagggtgaca attacctggg accttgacta ttgaaaggct 38700
aatcaggttt gattgggaaa aaagagttgc taaaaaggat tgtattggat aattggtgag 38760
atttgaacat ggattttata ttagtattgt gttattacat tttctgatat tagtcattct 38820
tttttttttt tttttttgac ggagtttgct ctgtcaccag gctggagtgc aatagtgtga 38880
tctcggctca ctgcaacctc tgccacccgg gttcaagcga ttcccctgct tcggcctcct 38940
gagtagctgg gactacaggc gggtgccacc acacccagct tgtttttgta cttttagtag 39000
agacagggtt tcaacatgtt ggccaggatg gtctcgatct cttgacctcg tgatctgccc 39060
gcctcagcct cccaaagtgc tgggattaca ggcatgagtc accgctcccg gccgttagtc 39120
attcttatag gtatgtaaga aaatgtcctt gaggtattta gcagggaagt tcatgatctc 39180
tgcagcttac tcacaaatgg ttaaacagaa tgagactaag aacagactag taagaaaaga 39240
ctgaagaata taattattag gccaggtgca gtgactcaca cctgtaaatc ccaacatttt 39300
gggaagctga gataggagga tcacttgagg ccaggagttt gagaccagcc tgggcaacat 39360
agtgagaccc catctctaca aaaaaaaaaa aaagtaaaaa ttacgtgggc acggtggtat 39420
acgcctgtac ttccagcttc ttgggaggct gaagtgggat gatcagagga cctcaggagt 39480
ttgaggctgc agtgagctat gattgcagca ctgcactcca gccagggaga cagactgagg 39540
cccggtctct aaaaaaaaag gaatctatgt gagtctatat attcgtgcaa cttttctata 39600
ggattgaaac tctttaaaat aagcttaata ataaaaacaa aagctggtgg gtgagacctt 39660
tcatgtactc tccaggagag ttaagccccc caacattcct gtccccttgt ttactctcaa 39720
gcaccccctc ccccacccaa ggaccaggtc tttgtttact gagcatctca gcgatgagct 39780
ctcaccccct gatttcatca attataaatg tgctcgctac tcaccacacg gcaatttgtg 39840
acggactgtg gtttgtggtg agagtagcac catccaagtt caccgcagcc gcgagtagag 39900
atgagggttg gggccagaca cagggctgtg ggggcggcaa gggcacgcag gcagccctgc 39960
caccttcctg tttgtcagcc aagtgaggct tccgagggca gcgggcgagc gggtcactac 40020
tcagggccag ctactgcggc caggccaggc tagtcaggtc tgtgcagcca gaactagagg 40080
ctccgccagg atgtgaggtc tcccagctcc tgggaactga agcaaacact ctgactaccc 40140
cttcttgaag tgccttacgg tgtatacatt tgtttaatct gcacaacaaa cctaggagca 40200
gttattgtta ctgtcctcat tttgcagatg aggaaactga ggcaaagaga aattaagtaa 40260
ctccttcaaa gtctggtaag tgacagaacc agattttacc ctcttactca ttgtccatat 40320
tgccgagtaa cttacattaa tagatactat gcatgtttat tttatttttt attttttttt 40380
agagatgggg tctcattctt tttttttttt ttttttgaga cggagtctcg ctctgtcgcc 40440
caggctggag tccagtgggg cgtgatcttg gctcactgca agctccgcct ccctggttca 40500
ctccattctc ctgcctcagc ctcccgagta gctgggacta caggcacctg ccaccacgcc 40560
cggctaattt tttgtatatt tagtagagtc ggggtttcac cgcgttacca gtatggtctc 40620
gatctcccga actcgtgatc cgcccacctc ggcctcccaa agtgctggga ttacaggcgt 40680
gagccaccgc gcccggtcct cattctttca ctcagactgg agtgccgtcg tgcaatctca 40740
gctcactgca gcctcaactc ggggctcaag caatcctgcc acctcagcct cccgagtagc 40800
tgggaccaca ggcagtgcca ccacacccaa ctaatttttg cattttttgt agagaccggc 40860
ttttgccatg ttgcccaggc tggtctcgaa ctcctgagct cagacaatcc acttgcctcg 40920
gcctccaaaa gtgctgggat tatagcacca cgcccagccg atactatgca tgtttaactg 40980
acacctaatg attaggtagc aacaatttct tggctgcctt attcaccttc atgaccccca 41040
acattgaaaa tgtcttttcc catgtgaaag catttgtggg ctcctactgc caatctataa 41100
agtagaaatc cttctgtggt acaaagccct ctttcttatc taatttccca tttcccgttc 41160
ccccttcccc cttctgctaa gagctcttcc ctacccacat tgtaagtagg gccggggagc 41220
taactccacc ttgcgtactc caaatcaacc acatgaccca ggcctgacca atcagggctc 41280
caaatcctct agctatagtg attggtttag ggagggacat gtgacccgcc tgagccaatg 41340
aggatctgtt ctgggactcc tgtttgaact cttgggaaaa taaactcctt atgttgggtg 41400
gctgagggga tgaatgtgag ctgggagctg caagcaggca tcatgctatt cccttcagct 41460
ttcaagtagt gttttcactg ctgttaacaa ttccaaatct taagtgttcc ttgcatcttc 41520
ctctcgccaa aaatattaca gtactggagg gcttacatgg tgtctcaaat gtctggagtt 41580
taaatctgcc ttgcagcttc catggccaca gtgagtgtct gagtcctggc cgctttctgc 41640
tgatcccggg gagagtttag cctgcctgcc cttcctccct ggttcgttct catgtactca 41700
gatccctgcc tcactcttct ctcacacaga caacaaaaga gagtcagatt gtggtcttaa 41760
gtgcacagga ctctgaagtt aggaccagaa gaccttggtt agagtctaaa ccttgtcagt 41820
taccaaatat cattaggcac attagttaat ctctctgagg cttatttccc catcagtaaa 41880
gtgggtactg ctgaagatgt agtctttaga agtgttctct agattgtaag ccccgtgctt 41940
cctggttatt gactaaacag ggataaaaat gagtttctcc tcacctgggt gtcattgagt 42000
ttctggtcat tgtgagatgg gtgatgaagc caagggcatc aaacatccca acgaggctgt 42060
tttctgcatc tccagggcta tcccagcgtt aatcacatat ggacctttaa tcagagtttg 42120
cagaataagt gagaattgtg ttagagtatg aatgtagata ttatcttgtt ttagtcattg 42180
ttactctagg ggaccattct tactacagga atattgcaca aaacccagaa atttattgac 42240
tttctcctaa ccaaggccta aagagctggt gttagacata ggccaaggcc agccagaggc 42300
ccaaagcctg tttcccaggg taggactgcc ctggcctccc cctctttctc cccaggctcc 42360
accccagaga gctgaagacc aggctgggta cggcactgct gagaaactga ggaaaaggcc 42420
actggcctcc tctctcactg caggctgccc acccgggagg gggaaagctt gtcactaaat 42480
caggttcagt tttggtcact gtcttggact ggatattcta gcatcagaac tgagatgttt 42540
cttgtgactt aaagtaactt caggactcta ttctacctag gattgggcag aaaagttatg 42600
ggcctgcggg agttccaatt cagaaacagg ggagattact tgcactaaag aaagtctaaa 42660
ggaaggtagg agacaaaaat aaagttgtgt attgatgatc ctaggagtta tgcttgtttg 42720
acataccagt tatacctgct gtcacggtag ttatgcatta ggggacccag gtgtctgaag 42780
ttatatccag aagacttctg agggtgcacc ggggggtccc ttggctaaaa gtgtgattta 42840
aaccctaaga gcctgcccag actatcagtc ccagtttcta cgtccactgt ccctgaatct 42900
cgctgcttct tccttaggct gctgggagtc tgaaccctcc cccgccaaca cccctccccc 42960
atgcctcagt cgtgggaagg gggggccctt gagcagtagg gccaagccct gttcagcctg 43020
ggaccaagtt cccatcaaca aggtggtctg ggcagtggcc agccagaaag cagtaattac 43080
tgtcgaggtg cagggacccc aggtagggcc cccacctccc acctctgtgt gggcagtgaa 43140
tgggcctgcc cctgggtaag gctgtgtcag caggcgcctg cccacccctt gctgggttcc 43200
caggccccta gagccctctc gtaataggag ccatttgcgc tgtaaccagt gggtgaccag 43260
atttttaatc ttggagaccc cttggatccc aggcgggaag tgggatttgt caaatgggga 43320
gaggcggggc tgtctgggaa tgccagacgg ggttgtgctg gggaaatatg tctcctttcc 43380
atacagcccc cttcccatac ctccagcctc cctctacccc gcaagtcagc tctgtagctc 43440
ctaggaggta tctccaacat gcttagctgt tgaaagtaaa tgaatgccgg aagttgaaat 43500
ctgaatggct tgttttgcac taactcaggt gcatgccaaa taggggtgtc tctttgcttg 43560
atcctaatcc ttcctcctct gaaatccttt ctgacctgct gcctatcagc aattgccctg 43620
caaagaccct ctagctggcc gcgggagaag ctgtgttctt ggctatcagg agtgagaaca 43680
ctggacccaa gcttgcctat tctctgccac aactcactgt gtgatcttgg acaagtcact 43740
tcctttcttt cagcctcagt ttcaggagac ttggcacttg ctgagtgcca atgtgtgcca 43800
tgttcttgac aagtatggtc ccacacaacc ttgccaatga ccctgtgacc tgtgtcttct 43860
tgttcccagt ttacaggaga gaagatgcag cctgggagag atgcagcagg tgtctgaggc 43920
cacacagcaa gtcacccagg gccaggatct gaagctgggt ctctccagct ccactgcctg 43980
ggcactttct cctccacagc gaccttcagg tcatcatgag gagcctttcg gactaaagct 44040
agagagctgg gattccaaca gttcagcaac ccatgacttc tccatggcag ctgctgcctg 44100
accacctagt gcctttccac taagattgtt ccttccctct cctgaagatt atcctcctgc 44160
ccctctctcc caaacatctg cggtgtgcac ctgctgccca aagttgaact tttttttgtt 44220
agagacaggg cctcactgtg tcatccaggc tggagtgcag tggcgtgatc atagctcact 44280
gcagcctcca actcctgggc tcaagtgacc ctctcacctc agcttcctga gtagctggga 44340
ctacaggcat gcaccaccac gcctggctaa tttttaaatt ttttgcagag acagggtcta 44400
gctatgttgc ccaccctggt cttgaactcc tgggctcaag caatctgtct gccttggtct 44460
cccagctcgc tgggattaca ggcatgaatc accatgcccg gtcctccaaa gttgaacttt 44520
tgagactcag tttccttctt ggtaaattca ggctcctagg tgctacctcc tcagagacat 44580
cctccttgac cacatcacat gacctctcgc agaggccaga ttctttaatg tattcatttg 44640
tctattatca tgtttcccca gtagaagttg cacaaacggc aggggcttca tctgttttgt 44700
ttgccgttat gtccttagca cctaaaattg tgcctggcac atatagtact cagtatgtat 44760
ttgctgggtc aatgagtgag tgaatttata ctaataacag cagctaccat ttctagagtg 44820
tttaccatat attgggcact gtgtcagtct tcccaacaac ccacagacga agatcaatta 44880
ttacacccat tgtacagatc aggaaactga gtcaggttaa gaaacttgcc ctaaatccta 44940
cagtctcact tagaacttct gactgcagtg ctcatcagaa tgcattgtca acccaaaggt 45000
catttccagc tcaggtggct tctatcaaaa gagctcatcc tggcctttcc aagagccaga 45060
cctccgacat cggtggagcc ctgtgcatag ctggcctctc ctgggcgtct tgtcccaagt 45120
acagagacct ggatcctttc ccactcatgt gcaacagccc aaaattaaaa acaaaagcca 45180
tattaaaaaa caaaaccaac tttctgcctt aaaatattgt gagccagggg gcaattagca 45240
attatgctgt attttattat gagaagatag aattctaatt ggactgattt gaattccaca 45300
cacctccaca gattgttttg ggaattaagg tatcagttgt atcggtaatt atggtttacc 45360
attcaattac ccccccacag aaaactgtta aattgtctgt gacggggctt aaatttagct 45420
cagacctatg tcctatgaag actgcgcgag tcaatacaag ccatccggaa accaccgggt 45480
gccctgtgcc aggcggtaat taggggttga ggtttccaaa gttttacctg agacagcagg 45540
gacaagtgcc tgggctgggc gtgctcacgt gggggggctt ggatgctccc ccagcacagt 45600
gctcttggct cctgccctgc gttgctggtg caatagctga tcatctggaa gacaatgtgg 45660
tttcagccgc aagtgacatt ttggcgaggt gcaccagcat agaagccctg agacagcgag 45720
ggaccatgta aaactcacgg acattgtaat tggacacatc tgggattgaa ttcccactct 45780
tccacttagg gagtgaactt gagagggcca cttaatatct ttgagcctct gtttccccac 45840
ctgtcaaatg ggtacacctc ctgcctcaca cacgggttat tgtgaagatg gatggagaat 45900
aataatggtg cctggcccaa attgcatttt tttctctttt tttagtactc actacatgcc 45960
agatacttta caaacatctc taaacctcct aagccctata gggcaggcac tgtggttatt 46020
ctctgtttta tggatgggaa aactgagtcc cagagaggtt aaataacatg ccgaaagtcc 46080
catgactatg aaatggggca gctgggatcc aaacccaggt gacctagggc caaaccctaa 46140
gcatgaagct gccctgctgg gtgccttctg tacctgtccc tctaagtgga agtgcctaga 46200
aatgacccag ccaaaagcag cagactgtat tttatcattt caacaattct ctctgtccat 46260
aaaaagttct atgcagcttg gccagttcct ttttcctgaa tacaaaactg agaatggtaa 46320
cagggaactg actttttaat gtcatggtgc aaaggagcta gctctgccat gacctccttg 46380
aagtgacctg gtagagtgag ggtaagacaa gccacactcc ctaggctatg gaggccactc 46440
tccatggaga taggggaata gggaatcctg caaaatacag tctctgggga tgggaaggat 46500
cagggaaggg gccaggtgca gtagctcatg cctgtaatct cagcatttcg ggaggctgag 46560
gtgggaggat cgcttgagcc caggagtttg agaccagcag tttgagacca gcctgagcaa 46620
catatcaaga ccctgtcttt acaaaaaatt aacaaaaagg ccaggcgtga tggctcacac 46680
ctgtaacccc agtactttga gaggccgagg tgggcggatc acctgaggtc gggagtttga 46740
gaccagcctg gccaacgtgg tgaaactccg tctctactaa aaatacaaaa attagccagg 46800
tgtggtggct gtaataccag ctactcggga ggctgaggca ggagaatcac ttgaatccag 46860
gaggcagagg ttgcagtgag cccagatcat gccactgcac tccagcctgg gagacagagt 46920
gagactccgt ctcaaaaaaa aaaaaaaaca attaacaaat tagatgggtg tggtggtgca 46980
cagctgtagt cccagctact caggaggctg aggtgaaggg atcacttgat ccaggtgttc 47040
gaggctatag taagctgtga tcaccccact gcactccagc ctgggtgaca gagtgagatc 47100
ttgtctcaaa aaaaaaaaat tttttttttt aaaggatcag ggtaggtaca tggactcctc 47160
ctattccttc tggcaccatc agtggttgaa cacacaaact ttggttcaaa tcctgcctct 47220
tccaatttgc tagctgtgtg accttgaaaa agttacttaa cccctcttat ccttagtttc 47280
ctcatcttca gaataaaaat agtgcttgcc tgatggggga gatgatggat gtaaagggcc 47340
tcatagaggg cttggcattt accaagtgtt tgagaaatat tggctgtttt tattcaaggc 47400
ctgctcattg ctagcatgag ctacctgcaa cagccacctc taggcctctt ttgctcccta 47460
tagtccagcc acaccaagaa accatgatgg cctgaatcct ctctgacttt gtcaggcctc 47520
cgtgccttta tacatgctgt tccttctgcc tagaaggcct ttctcttttt tcaacctcct 47580
tgagtccatt ttagtccctg ccagatggtc acttctctgt gatgctgccc taactccttg 47640
gcagccatgc tgctcccttg accattcact agccccttta attcagcccc agccacagtg 47700
aggattaaat gggatgatgt ctgagaaagc ccatgggcaa gaacctggca tggagtggtg 47760
ccccataaat gatgaatgga gctggggttg ctttctcagc cagctgcctg gcaggggcct 47820
ggcttcctca tcttcacacg tcagtgccta gagtaacacc tggcacacag tgaacacaat 47880
ctcagtaact gttagacatt tgtcttttgg ggagctgctc agcctcccac attggggtgt 47940
gggttttttg ttttgttttg ttttgttttt tgagacggag tttcattctt gttgcccagg 48000
ctggagtgca atggtgcgat cttggctcac tgcagcctcc gctgcctggg ttcaagtgat 48060
tctcctacct cagcctcccg agtagtagct gggattacag gcacgtgcca ccatgcctgg 48120
ctaatgtttt gtatttttag tagagacagg atttcaccat gttggtcagg ctggtcttga 48180
actcctgacc tcaggtgatc cacccgcctt ggcctcccaa agtgctgaga ttataggcgt 48240
gagccaccac gcccggccta ggggtgtggg gtcttttacc gactaggcct ggtgggggaa 48300
gtcagggacc ccaccacaaa agttggagag gccacatcct cccacctcca tctggggtta 48360
gacatgtgac ccaggcttgg ccaatcggat gttcctccat cttgcccaga attcacactc 48420
agcagtggtc ttcacgaggg gtggtagtgg tggccagtag gagcagggct gggcagcctc 48480
cctaacccac gttcatgaga cttgaagctg gctggtttcc tgtcccctgg cctcccttgc 48540
ccattcccat cctggtgctc cctccctatg gactcctttt gctcataaat atccctccaa 48600
ttaattgctt tgctgcttct tagctagtgt tgtttctgtc acttgcaacc aagagcctat 48660
tcaggtgcag gaagggttga atgaacaatg gaaatggatc aacacatgtt tctgagcatg 48720
tgctgacagg caggccctgg gcggagtacc acggtgttca aatgatgtgt aagatgtggg 48780
cagtgccccg gggggtctat ggggagacag accatgcatg acatcataaa caagaccaca 48840
gggtttagtt ggaccttgca gacacccaga gaagacgact ttgagccctc attggatggt 48900
gccatcgcaa agtacaaaac gaattgggtt gtaggaactg gaggcactgg gacaatattc 48960
cagattaggg attgagctga aggggttgga aatggctgag tagggtggtg gaaagggatg 49020
cctgctagta ggcaattgtc ttagtctgtt ttgtgctgct gtaatggatt accacagact 49080
gggtaattta taacaaacag aaatttattt ggctcatggt tctgaaggat gggaaatcca 49140
aaatcaagga gccggcatct ggtgagggcc ttcttgttgc atcataacat gccagagggt 49200
gtcacatggt ggaagggcaa agagagggag aaagggaaag agagggagca ggaaagggca 49260
aacccactcc catgataata agctcactca tatcatgagt cattcatcca tcgtgagggc 49320
agggtccacc tcccaatacc atcacaatgg caactaaacc tcaacatgat ttttggagag 49380
gacaaacatt caaaccatgg cagcagcgtt tgtgggaaac acgcgccaaa gcaagaaaag 49440
accaacctaa gagtgagtga cttggctcct cattctacat tcattttcat ccaatggggc 49500
ccaagggcat gtacccatta cccatctggg cagttccctt gaatgtgggc ttctgtttgc 49560
ccgtggaggt gaggaacttc aaggaagaaa ccatgaaaga cctcttgagg ctgagggctg 49620
gcaccagcac caagatctcc aggcagctgg aacagtgatg gctcctccgt cctcgcaggc 49680
ggggcaccca acagggtgtg accgtcacct gaggggagac agccagaggc acaggcctga 49740
tcctgggact gaggttggcg gtttgggtgg agaggtgatt ctgagtgtga caccctccag 49800
tgataaagtg gggggcttcc cagcagcccc tggggaaaca ggctgcatct ctggagacag 49860
gagatatgtg gaggcctgag gggcagtgga aagccctgtg tgtctgggtc aggtctctcc 49920
ttggcaggta atggtttgtc ccagtagttt tcagactccc catcctgccc tgtccttctc 49980
ctgtctgatg ctcagacctg tcactcccag ctcagccccc accttactgt ctacccctca 50040
gagtccctcc caccaagggc accttctgtc ccacatctcc atggtgcagt catgggagga 50100
gaacttggga gcataagaaa ctccaccaag ttggggccag gcatggtggc tcacgcctgt 50160
aatcccagca ctttgggagg ccgaggctgg tgaatcacaa ggtcaggagt ttgagaccag 50220
cctgaccaac ataatgaaat cccgtctcta ttaaaaatgc aaaaaattag ccgggcatgg 50280
tggcaggtgc ctgtaatccc agctactcac gaggctgagg caggagaatc acttgaaccg 50340
ggaggcagag gatgcagtga gccaagatcg cgccattgca ctccagcctg ggtggcagtg 50400
aaagacttgg tctcaaaaaa aaaaaaaaag aaagaaagaa aaaaaaagaa actccaccaa 50460
gggcttttgg gtcctaggga cagcaatgat gtggctggtc cagccagaac ctcattttgt 50520
cagtaaagtt gggacccata ttccctaaga gatttgtccc aagaacacat gggttgctac 50580
agcggaggag aaatcaagtc tgttttccct ctggtgaatt gtcccctgaa cgtgctttct 50640
tcctacagtg ttgccaggaa agtaaaaaaa aaaaaaaaaa aaaaaaaaaa gtccacgatg 50700
tcagctgggg tgataccaaa acaattgtgg gaggaacaac atccgcaaat tgaatagtgt 50760
gaggagtgtg gacagaagat gttttgtctt tggcctcatc tcccagactt gatctttgta 50820
aatacagaag tttccaccag agccgaacct ggcaatgact tgaggagcag ctgcaaggaa 50880
gacagcctct cccagggtat cacctggggg cacaccccag cttcccctcc tgagcctcat 50940
cgaggggtta gtgctacctc tcgggaaaac ataaagatga caagaagcca aaggtgccaa 51000
tagttcccat ttagtataaa agctggctca gcaaatcatg ctatttcagg gcctagggtg 51060
ggccagttcc caggcagccc tggcaggaag gactctgaga ggcggacagt aaggtagggg 51120
cttgggagtg ataggtctaa acatctgatg gaagcaaaag ggaggacaag ggagggagtc 51180
aaaaaatccg ggaagcctgt ccttccagaa agtaccgaca ccagggtgag gtggatccca 51240
gcacccgcac ctctgaatgg cctttcccct gcacccctat cccgccccca ctcggagcta 51300
ccccaagcac ctgttcctct gggccccaag gtgacgccct ttgtgtgtgt ataggaaagg 51360
acggtgtaca catttggtta gttccttctt ttacaccata aattatcaga gacaaccctt 51420
ttggaaagca atttggcaat aatatcaagt gacataaaga tgttcatagc ctttggccca 51480
ataatcttcc tcctgggaat taatcctaag aaaataattc acaaggaaga aagaaccatt 51540
ttgtatctac aaagacattt attggggtgt tatttatgat agggaaaaac tggagacacc 51600
ctccccagcc aacatagagg gatggtccta taaattatga tacatccatc caatgaaatg 51660
ttaccctgcc agtaaaaatg gtaaattgaa aattgtgtag caacaagaaa gagtgttcag 51720
aaaataaaag caagtgaaaa aaggagcaca ggattgtcta tatgctgtga tcaatggcaa 51780
tgctgacaaa tccatgtatg tgtagatagt tgtagtgtta cctgtaaggc acgataaggc 51840
ggatgtaact gtgcttcaaa tgttcttttc catagctgtg gagtccttct agtatgaaat 51900
catttttgta aaatcgcaat ttgtcaccag gggttttgac tttccttatt gcctcgtggg 51960
aggtggcaga ggcagcacat ctcagaccga gcctcggttc ctccccctac ctcccaccgc 52020
tgcgcttgaa gaaagatgct gcagcctccc cagtccccat gccagcgctc ccacttttct 52080
ctgagctttc ggtggcagac agcgccttgg gcactttttc atgctcataa ttcgaattac 52140
ctgtttaagt cggtcaaatg aaaaaatacc agctccgccc ccacgcgggc tggccggggc 52200
gcctggagcg ccagggcggc tgcagcgcgc tctccgcggc cgtcggccct gagctcattt 52260
cctggggcgc gcgcgccggg ctatttcagc ctggcgctgt gcaaacagga caatttactg 52320
cggccaaaag ggacccaaat tacaatcgta tcacagacaa atatccgcca cgccaggtct 52380
ccaggggcca ggaggggcct ctctcccggc gcggggggcg ggcgcggggt caggcaggtc 52440
cgcggggctc ggctcggcct cgccgtgccc tgatcggcgt ttgccaccga gctgtgcctg 52500
ctctctgcaa caggaagggg cccagctccc ccgggcgacc gttcctatct gagttctcgt 52560
tcctatctgg gttttcgtgc agaaaaactt catctcttcc cagggatttt cccctgattt 52620
agggtcccct tttatttggt ttcttttcag cacttgggat gaaaacatcc ctccatccag 52680
ccactccagg gctcaaagtc atatctcctc gttcagaaac ctcagtgcct gtccaccact 52740
cttccggaaa agtcggaatc cttagtgtga tggtcaaaga ccgctcttag tccagtccac 52800
ctgtccaggg ccagcccctg tacccagatg ctccaaccac ggagagcaca tcagactttc 52860
acaattttat ccttgacaat tgccttttca tcctgcctaa atgttccctt gtttctttct 52920
gcctagtgag ctcttattcc ttctttaaga cccagataca catcacctct tccatgaagc 52980
cttcccagac ttcccctttc cctgcttgac atctgccttc caagcagagt cagccccttt 53040
tccttcctag caccgtgcag aggtagatgg tacttctcac atcctgtaat taattgcttg 53100
catgcctgtg gtacaccgta ccattgctca caaatatttc ctcttcctct ctggagaagg 53160
attacacttc cttgatccat tggcctcact caggcttcgc cacatgactg gtgctggcca 53220
gtgaagtggg acatgtgagt tactggggag cagaaacttt caaagccaag catggctcac 53280
cttgttctct cttccctctg ttacaaggat ggcagtgccc cagccagaaa ccacactgcc 53340
agcctggcat aggagtggag cagggcacac ataagtgtga gagaaatgaa cttcacctca 53400
gtgctcgagg cccctgggac catggactca tttgttactg cagcataaac tagctggtct 53460
tgacttcagc ttctgcctct cttccctggc tttgccttca caaaatcaag agtctgatct 53520
tactcatatt atagagctgg tcttcaacca tgttcttttt ttttttttcc tttttttttt 53580
tttgagatgg agtttcgctc atgtagtcca ggatggagtg caatggtgcg atctgggctc 53640
actgcaatct ctgcctccct ggttcaagcg attctcctgc ctcagcctcc caaatggctg 53700
ggattacagg tgcttgccac cacgcctagc taatttttgt atttttagaa gagataggat 53760
ttcaccatgt tggccaggct atctcaaact cctgacctca ggagatctgc ccgcttcggc 53820
ctcccaaagt gctggcatta caggcatgag ccaccatgcc cagccccatg ttcttcgaat 53880
ggccaagaat atcagtaggt aaggtttgtt gagcatggat tgtgtgccag gtgctgtgct 53940
atgcctttgg caagcgttat cccattacat cctttaacag cactctctgg tgggtactgc 54000
tcttatacaa gatttacaaa agagaaaaga gactcagagg aatgtagggg ctcacgtgag 54060
attgtaaagt agtgagaagt ggagctggtc cttcagctca aatccctctg atgccaaaga 54120
actcaaactc tgcagttcca tcatgtgcat cctcactctc tcacttcctg cagaggatct 54180
gtggaggttg taactattgt tttcacctgt ctcttccact gggctctgag ctgcccgaag 54240
acagatcttt tttttttttt tgagacagaa ttttgctctt gttgcccagg ctggagtgca 54300
atggcacgat cttggctcac cacagcctct gcctcttggg ttcaagcgat tctcctgcct 54360
cagcctcccg agtaactggg attacaggca tgcgccacca tgcctggcta cttttgtatt 54420
tttagtagag acggggtttc tctgtgttgg tcaggctggt cttgaactcc cgaccttagg 54480
tgatctgtct gtctcggcct cccaaagtgc tgggattata ggcgtgagcc accacgcccg 54540
gccccgaaga cagatcttaa tcatctgatt tcccatccat taattcagtc tttcaccaag 54600
gatgtattga gtgccaatta tatgccaggc atcatctgat gtgttgggga cagagaaata 54660
aaacagataa aaatccctgc tgttaattct agcctatggt gacaagaagc agatccgtgg 54720
ctgcctgggg tgagtgtggg gtaggaaatg ggagaaatgg ggatgactgc aactttgggg 54780
gtagtggaaa atgttcagta tcgtggtagt ggtggtggct gtacaggtat atatatccgc 54840
atacatccca cagttcagaa ttatacactt taaatggtaa atggatacag tttattatat 54900
gtaaatgatc cctcaattaa gttgatttag aggaacctgg ccgggcgtgg tggctcacac 54960
ctagtattct cagcactttg ggaggccgag gtgggcggat catgaggtca ggagatagag 55020
accatcctag ctaacatggt gaaaacccat ctctactaaa aacacgaaaa caaaaaatta 55080
gccgggtgtg gtggtgggcg cctgtagtcc cagctactcg ggacactgaa cctgggaggc 55140
ggagctggca gtgagctgag atcgcaccac ttcactccag cctgggcaac agagcaagac 55200
tctgccttga aaaaaaaaaa aaaaaaagaa cccttgctct tatggagaag cattctactg 55260
gggaaaacag acaataaaca catagataaa taaaacatat gtcagaaagg ggtaagtgat 55320
gctcagaaaa ataggacaag ggatcagaag tgctaggcgt tgcaatttaa aataatgggg 55380
tctggaaagc caccctgaga agggccaatg gagctatgcc cagcctgtgg tatccttcct 55440
caggcagggg actgtacccc cagcttacaa gccttttcga ggcaccttca gggtttgagc 55500
ccaaaatgca ctgcacatta agtgttattg atgggcttgt ggaaatctgc tctccgtcag 55560
gctctgatga aacttttcca acaggagact gaactcagta ctgggcatgg ccccctgcac 55620
atagtaagtc cacagcaaat gtgtgtgggg tgaatactgt cttctttatc ctcccttttt 55680
ctactccagt ccaggtagac ggtgtatcta cccacccaat tttgctgttt ctggtaccct 55740
cagtgggtct tgcctgctct tcctccttga aatcattact cagaggtctc cgtgtcagct 55800
ccagacagat gggcctggtg ctcctttatc tcaatacaat tcccccgctc ccaggctcca 55860
ggggaaatcc tgagctaact ttggtctttc ctaaactcca cacttccctt tgggcacctc 55920
tttccccatt gcggggctcc ctttcagaat ttgctgcttt atttatttat ttatttttgt 55980
cagaggtgca ttaatgatgc tttatttaaa aacaaaaaac ttggccaggc gcggtggctt 56040
atgcctgtaa tcccagcact ttgggaggct gaggtgggcg gatcacaagg tcaggagatc 56100
aagaccatcc agaccaacat ggtgaaaccc agtctgtact aaaaatacaa aaaaaaaaaa 56160
aaaaaaaaat cagccgggcg tggcacatgc ctgtaattcc agctactcag gaggctgagg 56220
caggagaatc acttgaaccc gggaggcaga ggttgcagtg agccgagatt gggccactgc 56280
actccagcct gggtgacaga gcgagactcc atctcaaaca aaaaaacacc acaaataaat 56340
aaaaaataaa aacaaaaaac caaaaacagt ccattccatg tcgtgttgaa actgatcagt 56400
gtaagttaaa tggtggtttt taggctggac ccatgattta agctgtaccc atccagctca 56460
aactgaaaaa aaaaaaaatc atttgaatgt taaagcaatc gttcagagtc ttcaagaaga 56520
aaccaggcag gaaaatgcca ataatgatga ctggcaaaat caaaatctaa aacaaataaa 56580
ctgtttatca agctgccgac agaaaaagaa atcttgcatg gagactacaa gtctggattt 56640
tctgggatga aattgtacag gaatctcagt ctacagtttc ctcaatcgct gtggagatgg 56700
agctgtcact gaatctgaca gagccctgca ctccccagtc cgccgaccct ttctgtaatc 56760
cagtcttcac tgtagcctga ggaactattt caacctgctc cttttttatc ttcttctttg 56820
gcacaacctc agtggacttc tctgattcag aacaagttct aattgatctt ctctgttgct 56880
tcttttctac tgagcctgta gaaccagatg ttgcttcaag agatgatata ttctgcattg 56940
gcttttcatt tctctggttt ggtttagaaa ttataagcct gtcttgcccc ctgacactta 57000
tttctgtttt gttaccaatt ccctttgttg aataaacaaa ttaatttccc atcctctgta 57060
gcattctgaa gagcaaacac ttcttcaatt ttcacagctg gagacatgtt acacttctgc 57120
aaatccaggc tccctttgtg cattgtaatg gaagctggta ggatttcctt gctgccacag 57180
ttttccaggc tattttaaca ggaggtggct cttcctcgtc cgcgcttgtg tgctgcctcg 57240
ggctgtgtct ccaaatgtca gtacttgaga gtgaggaggc cacctcccct gcattgatct 57300
gttctggctg agtttaaagc acagatcttg gtcatcaggt ttttttaact tcggctttgg 57360
agacaacatt cttttttttt ttttttttag atggagtctc gctctgtcgc ccaggctgga 57420
atgcagtggt gcgatctccg gttcatgcca ttctcctgcc tcagcctccc gagtacctgg 57480
gattacaggc gcccaccacc atgcccggct aatttttttg tattttttta gtagagacgg 57540
ggtttcaccg tgttagccag gatggtctca atctcctgac cttgtgatcc gcccgcctgg 57600
gcctcccaaa gtgctgggat tacagacgtg agccaccgcg cccggcccca acattctttt 57660
ttgcttggga taaaccctct tcaggctgtt aatcaatata gataaaagta tactgttcta 57720
ttctttcttc tcaagtcatt ttcaatgctt tctctgcatg ggcaatgcca aaatcccatt 57780
gagcatgttc tctctgaggt cagggtttcc aaatcttttg tttctcagag tgattgctgg 57840
cttgtttggt tgcctcagcc agtaattctt cataccgctt atgaccttta tactcctgta 57900
cccatttttc atgaacccac accctctctg gctgtttgct aaaacactgg acatgatatt 57960
ttcgggcacc tcctgtgtta attttggtat gaacctccag ctggggatca cttcaatcca 58020
tacaaggcca gcacggatag gttcccacct tggaccacac aagatcacca acttgaaact 58080
taacaccagt ggacacttct gttgttggaa cagaagatag tattggctga actggggctt 58140
cctcttttag tactggatct tcccttggtt tttctgatat ggtgtgaacc ctctcatttg 58200
gtctattttg ttcctctggt tctaatttgg ggattttgtg tgacttgcgc tcttcagatc 58260
ttgatgagtc atgctttttg ggttttttcc tcttttcttt tctgctttca tgctttgagt 58320
tcgtgtgctc acttgcctgt acttcattta aaaggtctcc acaaagggaa gacttgaaca 58380
attccctgcc attctggata gttttggtta tttttagttt aacttcaggt gagccactct 58440
tctttggaat cacagtttgt ggtaccgaag tggtggctgt ggaggggaag ggttttctag 58500
aatttaatgt ggtcttctgt ttagaatttc tgaatggtaa tagtcagtgg ggctaaagtt 58560
tctagctgcg ccaaagtcat tggctgaccc attaggatat tgattatgcg actggtattt 58620
ggtttgaatt tcatacatgc tgattgatgg caggtagcca tttgtgagtg gaggaagatc 58680
tgttgtaggt gagtattgaa agctttgctg caaggtagct tcgtatggtg tctggccacc 58740
atcttcagca atgtcacttt tgttatcaaa ggcatcctcc tgacggatgc tggcggagtc 58800
agtgagttga ggtggttgtt gaactgtgtt tcccgtgatc ccttgcatga aagagaaaga 58860
gaaatccatt gttctgctcc agcattctta actttccctt tctctcatcg ggccttcagt 58920
ttgtcccgtt ctaaccgccc ggccgtctca atgcgatcac aggagggaca cggagatcga 58980
ggagggccac agccggacca ggccaggcgt gctgcggcag gaggaaggct ccggaggtgg 59040
ggtgggaagg ggaggccgag agacgggtgt cgccgcgccc ccgctgccgc cagagaggag 59100
cctacggctg gcagcctggc ctgggcagca gggtcctcgg cgctcggctg ggaaatcgca 59160
cgtctctccg cggtgacctg tgcacagccc ctgggcctcc gcctccgtgc tggcagcctc 59220
cgcctcagcg cacaaagccc cgtcaccccg actctcggag cgccgccgcc gccaaatcct 59280
cagcccctcc ctcattggcc gcggcgtctg ccgggaagtg cagtcccggg tttggggcga 59340
tggagcccag gaggaagcgg cgagtcagcg cggcggagag ggcggagggg acggaggggg 59400
cggaagggga cgaaccacga acgcccgcgg ccgcgaaggg tctggacgac aaaggagaga 59460
ctagcgagag ggcttgttgc tttttttttt tttttttttt tttttttttt tgagacggag 59520
tctcgctctg tcacccaggc tggagtgcaa tggcgcgatc tcggctcgct gcaacctccg 59580
cctcccgggt tcaagcgatt ctcctgcctc agcctccgag gagctgggat tacgggggcg 59640
cgcctccacg cccagctaat tctttgtact tttagtagag atgaggtttc accatgttgg 59700
ccaggctggt atcgaactcc tgacctcgcg atccacccgc cttggtgttg cacttttata 59760
actcttttta ccttctcacc aaactagcat ttaggaaaaa aaaaaaaatc aacaaaaaca 59820
aaacaaaaaa accaaaaacc tctatcagtc atttacacgt tagttggaaa taatttagac 59880
cagtgtgtct ctccttcgca gaacacattc agtcctctct gattattaga agcaaggttc 59940
accttttgat cttggttaaa gaaatttcag gctaaatttc tggggaggag atgtatttca 60000
ggttccagga tcccagaatt gctctgcaca agtcacagcc atccttgttc cctgaagtct 60060
ctgttggctg caccatgtct gctgctcctt tatgagtggt tttgggagag gccaggctga 60120
gctcttacta gctccttgca acaaaacaag cgaatgtgaa aagtcttaaa cactcagctt 60180
tctggtcaga ggaaatccca aaatcagcat gactcaggat tttaagtgcc ccatagagtg 60240
tccaagagaa gaaacctagg agtctagggt ctttcaaaac cccaaagtta aaacctttta 60300
tcaccctttc ctgaccttcc tggcttagga ttttaggcag cctcttggag gcctcccttg 60360
cctggggcac acttggccat aaacccattc attcagccct actgatttag tgcccattat 60420
gtgccaggca gaggaaaagt agaaatggta ggatctatct tcaaggggct caaagtctgc 60480
aggaaacagg atccgctaac aaattagagc acaagggtgt gccgagtgct ataatagcag 60540
tgtgggttgc tgttgagaaa atacagagga gggtaccacc agggagcttc tatgagttga 60600
gctgagcctt gaaggttgaa gaggcatttc tcacatagag aagacgaaat agtaccctga 60660
ttgtatacta ggaatgaaaa caccaagtgc ttaccaagct cagataggta acgtaaatga 60720
atggagtagg tgagtaaagt agcagggatt gtcagcggtg gtggggaagt ggaaggcctg 60780
catcctgcct acagggcttc tgctggaaag acagcctggc ctggattcag cctccatttg 60840
caccttctgc taggtgcttt tgtgcagtgc acaactcaca caacctcaca tggctgccct 60900
gcctgcctaa gctggccatc agctgcttgt tgccatactg aaaaagaggg tttggttttg 60960
cctcgccttg ctttttcaat agaagccaga aacttgaatt tttatgtaaa aatttccaat 61020
ttgtcaaatg ccattgtagc caaacaaaaa tctagcctca ggctggatcc accatagagt 61080
ttcagtttga gaccccgatg ggaaccagtc ccctgggcac atgctgctct ctctcctcca 61140
taccatgcaa cacagtcctt tgctgagatt tgacaaggga aacacagtca tagatgaaac 61200
cccctacagc tgctgcaaag cccttcagaa acatgagatt ggcctctgaa ccagaggaaa 61260
gtaacgtggg gctgtacaac agcagccagc ctgtgagact gatggataaa gctttaattt 61320
atcggtgcac cttgtttgga gaaagcttcc ctgccctctt ggctgccccc acctccaacc 61380
cctcctgttt tgctaatgat aaatgacaag cagtggacat ttattaatta aaccacccgc 61440
tgtagccatt ggtaaaaccc aaggccacat gcaggggaag cattcaaagc attcctgctg 61500
cccctgccca gggcacaggc cccagggtta tcctttgctg gagagagcag gccacggcgg 61560
ccacagcctc acatgatggg ccttcctagg atctgtctgg gttctggctg ataaatggaa 61620
atcacctcca tgggccacac agtaattaaa ctcctggcat tcttttgaca aaaaaaaagt 61680
tcctcatggg acattcccac aagctgctga aggtctggga cctgcaagct cccagctgat 61740
taccaaggag ttttcgaagt tggccttgac tgaggatcaa aggaggatgg gagttcaggg 61800
aatgagggtg ggggtgggaa atgccttaga attaagttga tgatggcctg ttgcggtggc 61860
ttatgcctct aatctcagtg ctttgggagg ctgaggcggg aaaatcactt gagcccagga 61920
gtttgagacc agcatggaca acatggtgag accttttctt aacaaaaaat ttaaaaatta 61980
gcctggtgtg gtggcacatg cctgtggtcc catctacacg aggaggctga ggtgctagga 62040
ttgcttgagt ccaggaggtt gaggatgcag tgagctgtgt ttgcaccact gcacttcagc 62100
ctggtgaagg aggagaccct gagacagaag gagaccctgt ctcaaaaaaa agttgatcat 62160
ttggatgttc ggcctgaaac acccacattt atttccccag gaccgagatg ggcagagaga 62220
taaaagcagg ccttcaaggc tcatttattt atatttctac tgtttatttg ttccactgat 62280
gtaccccaag tacctaaaac agagcctggc acatagtagg tgctcaataa atatttgttg 62340
actgattagc tggagatgtg ctcatgtctt tttaacgaaa gcagtaatca ggagaaattc 62400
ctaattaagg tcctagaacc agtggcttct aattgtttgc tgccacagga ttgtaaacat 62460
gttgaagatg aggccagtgt cttattcaat tgactgtccc cagaagcagg cacagtgccc 62520
agcctagggc ctacacaccg tggtttctca acgatatcaa ttgtctgaat gactcagggg 62580
taaaccattt ttcagtgctg ggtcaggcag agccccactg ggacgtgacc tgcacacact 62640
ttggcagtcc cctgggatgc agtggtagca gcagcagagt cataaagcct ggggatcaga 62700
gccacactgg atatcacagc tggaaaggcc tgagatctca gccagtccct tgctaagcag 62760
aaagaagaaa tgaattagct acaaatcttg gggcctctaa aaacacagct tcatttttct 62820
gtttctccta tagactcccc attctgtcca actgccaaaa acatttaagt ttatttaata 62880
atcaatgtca gctgaagttg gtcatatcag acttaaattc aatctattcc cagttgcctc 62940
aacttccctt gtcaggtcat ctacttagtt ctagtggctt ctgtgccata ggagggggag 63000
ctggaggagg gggagagccg agccatggtc atcagtatct gtcttcacta acctcttggg 63060
catggtcacc ccattttgtt ggtgtctgct aaatccacat cttttttttc tgtagtaatt 63120
agcccacaca ctctgtgtcc tcctgactct gcagctgagg ccctcccagc ccaccaggcc 63180
ctgcccagca ccttccatgc cttccttgga agtgttggaa aactagctgg cccctcatac 63240
tatagggacc cagggaagtt ctgattgacc ctgggtgcca tgatagaaca ggactccacc 63300
cactcagcat tccagataaa cagtggacac agtctccttt gcactcctat tttcccaact 63360
ggttgggagg attctgttgg tgcctgctcc ccaccctatt gggatctctg tctccctggt 63420
gagctgtgta tgtgcatgat ggtgagggtc tccagcaaca aggaaaggag ttacatttgt 63480
ccatattcac actgctataa agaaatacct gagactgggt aatttataaa gaaaagaggt 63540
tcaattgact catagttcca catggctggg gaagcctcag gaaacttaca atcatggcgg 63600
aagggaaaag gcatgtctta catggtggca ggtgagagag agtgtgtagg aagtgaaggg 63660
ggaagagcat cttataaaac cgtcagatct cctgagaact cactcactat caccagaaca 63720
gcatggggga accaccctca tgatccagtc acctcccagc agctctctcc ctcaacacct 63780
ggaaattaca attcaagatg agacttgggt ggggacacaa agcctgacca tatcagaggt 63840
tgatcttgca gcattttatt ctctgaatgt cacatgggga aacggaggtc aagaaggtgg 63900
aaaagacttg cctaaggcca cacagcatga gtgagtgtgc ctaaggccac acagcattgg 63960
gccaagagcc tgggacttgg taagcccctc ctgagccctc ctcctgagcc aggccctgtc 64020
tcaggttcta gcacatgcaa ctgcaaccag cagggtatgt gtcatagtct taaaatatgg 64080
ccacaaattc tttgatggtc ttcccattga gctgtggggt ctatttctct cttttctttg 64140
aatattggca gggttatgac tgcttcaatt aattgagtac agagaggtga tgctgtatga 64200
cttccaagcc taagtcatct caggccatca gcttctgcct ttgttaatgg aacacttgcc 64260
tttggagtcc tgagcctcca ggtaaaaagt tcaactatcc caaggctacc atgctgtgag 64320
gaagcccaaa ctacacagag gggccatgtg aaggcactct ggttgacagt ccccactgag 64380
atgtctcagt ccaggcacca ggcatgtgag ggaagaagcc cccaggtgat ttcagccctc 64440
agcctttcca gccacctcta gccatttgag tcttcacaac cctggcccta gacattggag 64500
agcagagaca ggccaccctg ttgggccctg gccaaattct taaacctctg aatctgtgag 64560
cctaataaaa tggttgttgt tttatgtcac caactttggg gtggcttgtt acacagccac 64620
agatagctgt gccatcttaa cctgctggat tggtacccct taagtctctg atatccatgc 64680
ttctgcccgg ccactaatga agccacatct gttcccacca gaagaccact gagaaggcca 64740
gtgagtgtgt ggtggggacc cttgtttgaa ggaagttggg tgaaggaagt tgctgacatg 64800
catgactgct aacaaaagaa tatacatctg cggaggttgt tctggaagta tcttagggaa 64860
gtaaaggaaa ggaattaaga ctcatcgaca acagactcca ttccttgccc tgcccatcct 64920
tattcattta ttcaacaagg ggtctatgag caactacgag gtgccaggcc ctgtgttaag 64980
attctgggac atggacacag gcctggccct agtggttctc ctagtccagc tagagacaaa 65040
cattaaacga tgattaatat acatactttg aatgaaaaac ttagggtgtt ctgagagtgc 65100
gtggagacct atttaatcct cataatatcc ttctgaggta ggcaccatcc tttctatttt 65160
gcaggggaat actaaggctc agaggggtta agagaccttc ccaaggtcac ccagcagggg 65220
agacaccaga ttgcaaagaa gcagccccca cccacctagt gaggccagga aagcagaggc 65280
ataaatgtcc agctgagcat gagactcctt gggccaacag gagcacggtg gggtcagcac 65340
cctcagacag gcaacctctg agggagcagc attaacccct gggaagccag ggtagcttgt 65400
taatccagca aacctgcact tctcaagaaa aaatggccct ctgttgggaa ggaatgattg 65460
agctctaatc atctagcagt ccctgggcag ccacactcac catctctata atgagggcag 65520
aaatagaggg caagaggtat ggtaaccatc cagaggcagt gactgggact cctaaacccc 65580
tccttgattc tgagtcccca ggggccagtg gaggggcatt gggaggtgag acaagacatg 65640
cagacaaccc gcctccaaga gatgtcatag agaagtgggc ttcagacctt ggggtgtgga 65700
agcacctgga gaataggtga aaacgctgat gcttggcccc accctgaggg atttgggttc 65760
atttggtctg gggcgaagcc cgggcatctg tgggtttttt tttttttttt tttttgagac 65820
agtcttgctt tgttgcccag gctggagtgc attggcatga tctgagctca ctgcaacttc 65880
tgcctcctgg gttcaagcga ttctcctgcc tcagcctcct gagtagctgg gattacaggc 65940
acctgctcac acacctggct aatttttgta ttttgagtag agacggggtt tcaccatgtt 66000
ggccaggctg gtctggaact ccagcactca agcgatccgc ctgcctcggc ctcccaaagt 66060
gctaggatta caggcatgag ccaccgtact ctgccaaaaa tatatatctt tagattagct 66120
tttaatagag ttcttcaaat acccaaaaga ttagggaaaa ctaggatgct aaaagatgaa 66180
ataggtgact aggcaaataa atcattaggg aaattggttt caggctcatg gtattgttcc 66240
ccacaatgct gggcaagtta cttgtccatt ccaagtttca ttttccccta cctgaacaat 66300
ggagctaata aaattgattt caccgggcgt ggtgggtcat gcctgtaatc ctagcatttg 66360
ggaggccaaa gtggggatgg atcacctgag gtcaggagtt cgagaccagc ctggcccatg 66420
tggtgaaacc tcatttctac taaaaaaaat acaaaaaatt agccgggcat ggtgtggcat 66480
gtctgtagtc ccagttactc gggaggctga ggcagagaat tgcttgaact tgggaggcgg 66540
aggttgcagt gagccgagat cgcatcactg cactcagcct gggtgagtgc agagtgagtg 66600
agactctgcc taaaaaaaaa aaaaaaaaaa tttcacagtg tagttttgag gacgttgtaa 66660
gagggcgtga gttatgtgcc aggtgggtgg taaacgttca atagatgcta ctttccttcc 66720
ttcctgagca tttggaccac acagagacct ccctgctctt ggctttggct ttaaggagct 66780
tgtggtcact gagaggattt tgcactggaa gtacatgcat tcaaaatgga tacctaagtg 66840
tatattttct ggtgtaaact atatgtcgac tctaacttta gcccggggga gctttattat 66900
ttgtctccct ttcatgaaag ctataataga ggaagagaaa accctgcctc gcacattccg 66960
attcctaaat acataattta taattttctg ggatattatt taagtttatt ttagttctgg 67020
atacacacca tccccgtggg gtgcttattt aagtatcggg tgggctctgg aaaggcctgg 67080
aatgcctcaa aaggagggaa gaagattctt tccattcatt aaacagcagg gcctggggtc 67140
tttgcaggtt ctagggctta gaagtttcct taacaccaac tgaggagtcc cagggtggga 67200
gctgagctca cacgaagctc tacttcgcgt gtcctgaagc tttgacagtt gggcctcttt 67260
ctggcttttg catcctctgc tcatactaag gccagcagag ctacaggttg gaccatggtc 67320
tggccgggag ctccagcttc ctcttttcct ctcagtagtc atcgggccag ctgcccatac 67380
ctggtgccca ggtatgagaa gagacctttg gctttcacca ggttcttgag agggtcaggg 67440
acctccaaaa ggataaatgc cattggtcag atagtcactg tctacctccc tcccatccat 67500
ttcctgcctt caacccctgt tcttgggaca aggccttcgc tggcattcaa tggcgggtcc 67560
ctgaggaagg ccccagcctt gccgaacatc cttcaaggga gacttcagcc cctggccctg 67620
cagtgagtgt gttgttagca gcagaggggc tgagtgaggc agtgggatgg ggcttttctt 67680
ttttgttctg gttgcaaatt ataaaccccc aaatgctgga gttccagact gaggttcaca 67740
aactttagag ggcagaacta aagtcaaatg tgcttttaaa agcaccccag gagatgctga 67800
tgtgggtgga cacttgaaga aacactggtt gataaagttt ctctctttct tttttttttg 67860
agatggagtc tcgctctgcg gcccaggctg gagtacagtg gggccatctc acctcaccac 67920
aacctccacc tcccgggttc aagcaattct cctgcctcag cctcctgagt agctgggatt 67980
acaggcgtgc gccaccatga ccagctaatt tttgtatttt tagtagagat ggggtttcgc 68040
catggtggcc aggctggtct cgaactcctg acctcaggtg atccgcctgt ctcggcctcc 68100
caaagtgctg ggattacagg tgtgagccac cacgcccagc catggttgat agtttctaga 68160
aagctgttcc aagttactgg ctgtttttga attgttcaag cttaaaaaaa agtaggcacc 68220
ctttaggaag gccagtagaa gtcccattcc caaggagggc aggcctgtga gcacgttaat 68280
ggcaacagtg gcagctaaca accacttttg tagccctact gtgccccaga cactgttcta 68340
agcactcata aacattaatc taaacctccc aacagcccca catgccagga cacccgtgcc 68400
tctatttcag gaaaggccat ctggatcttg caggacggca aatgggagag gacaaacaga 68460
gccccatagg tgaaaaggag gaacgagagg ctggagagga atggggagca ttagagatca 68520
caaaatgtgg tagacagaat aatggtcccc agatgttcgc atccacatcc ctggaacttt 68580
tgaacgttac cttatcttac cttatgtggc aaaagggact ttgcaggtat gattacgtta 68640
agaatcctga catgtgaaga tcattctgga ttatccagat taatccaggt ggattggggg 68700
aacccagtgt catcacattt aagggtcctt agatgtggaa gataggggta gaagggtgag 68760
tcaagggagt gccatgagag aaggtggaag gggtcacaag ccgaggaatg caggccactt 68820
ctcggatccg caaaaggcga ggatatgtgg tctctccaga taggagcaca gccctgctgg 68880
aacctggatt ttagccccat gagacccatc ttacacttgt gacctttaga gctgtaaggt 68940
cataaatttg tgttgcttta aggctctaag tttgtggtaa tttgttgcag cagccataga 69000
aaactagtag aaggaagaaa aggagcatta agagagattt cccactttgc tttgaaaagt 69060
ggctagaaat agatggggaa acggggagaa gatggcccag agcggaacgg gggagaaagt 69120
cttcttctat gactaaggaa gagtgaggct tctaaacggc cctcaggaaa gtgtttgtgc 69180
catgatgctt ttcaggagag agtagagatc aattgatctc tgtgctgtgt tcttacatga 69240
aatatttggg agggaagtgt tgtggtgagt cacttgtatt tttttttaaa tacctgccac 69300
tcccagtttt ccagatagct gaggaaaggc tggggttcaa atcccataaa aaaccaacat 69360
cattcaattg gtacattctc taaaatgtac ataagacatc taggaggact tcataatgat 69420
taagagcaat caactgttaa tgcagtgagt gcttattttt tccaggcact gtaccaggca 69480
tgctgcttca tttaaatttt gcagggactt ttgaagtagg tcctaccttg atttccattt 69540
tgcaagtgaa aagcatcaca ttgagatgtg gcccttcagc cttagccctt tgatatggtt 69600
tggctctgtg tctgcaccca aatctcattt tgaattgtgc tgccataatt cccatgtgtt 69660
gtgggaggac ctggtgggag ataattgaat cgtgggggca gtttccccca tactgttctc 69720
gtgatagtga ataagtctca cgagatctta tggttttatc aggggtttcc actttagcgt 69780
cttcctcatt ctcactttgc ctgctgccat ccatgtaaga cgggacttgc tcctccttgc 69840
tttctgccat gattatgagg cttccctaat cacatggaac tgtaagtcca attaaacctc 69900
tttcttttgt aaattgccca atgtcaggta tgtctttatt agcagtgtga aaatggacta 69960
atagaccctt cttcctcctt gctaaaatac ttttttgatg gtgatcatga attggccttt 70020
gcgtgcagtg agttttgact ggtctgccaa tgcctttcct ttccagctat gaatggtttt 70080
aaaaaatatc aatgacaagg ccccacctca taaccattaa atgcaaaact ctggtagggg 70140
cccaggtgtt tcttttcttt tcttttcttt tctttttttt tttttttgag acggagtctc 70200
tctctgtcgc cagggtggag tgcagtggca caatctcagc tcactgcaac ctccacctcc 70260
caggttcaag agattctcct gcttcagcct cccaagtagc tgggactaca ggtgcgtacc 70320
accacgcccg gctaattttc tgtattttta gtagagatgg agtttcacct tgttagccag 70380
gatggtctca atctcctgac ctcattatcc tcccacctcc gcctcccaaa gtgctgggat 70440
tacaggtgtg agccactgtg cccagctggg cccaggtgtt tcttaaaagc tctcaggtga 70500
ttctaatgtt gcagccaggg atgagaccat ttgtctaagg caatctggat aactcctttc 70560
ccattagcca gggattagcc tgtgaactat tctgaccaag gaggcatgaa cttttctgtt 70620
tctggatgtt gtcatctgca aataacagga aggaacctag gatctgaaga tgctgctgag 70680
ctgcctgagt agctcacctg gatccaccct atttctagac ttcttgttat atggtgaata 70740
aatgtcctcc tggtttaaga caagggttgg caaactatga cccaggagcc aagtctggcc 70800
cactgcttga ttttgtaaat aaagttttat tggaatacag ccatgcccat ttgtttatgt 70860
attgtctaca tattgtttag tgctacaatg gcagggatgc atagtcatga cagagacctt 70920
atggcctgca aagcctcaaa tatttactct ctggcccttg gccctttgca gaaaaagttt 70980
gccagctcct ggtctaaggg tctttttgtg ggcctttcta ttacttgcag ttgagggtat 71040
ctgttaattt gggagtttag cttccccaag gtggcattgt tagtgatggc aagttttgcc 71100
ccctttaagc cattttttca tgggagaata attacagaat gatctttaag ggatttcaat 71160
aatatcagcc aatcctactg cccatgggga agtgggatca cacaggttgg ggcttcagat 71220
aggcctggat ttatggcctg gttttatcat atagatccaa ttgtctttga aaagtgactt 71280
catcttctag tcctcaactt tctcatctaa aatgaggaca gttatatcta cacgaaggat 71340
gggtaccctc atggaggcaa ttccagacaa aggaatcagt gcaagcaaag gaagcgaggt 71400
gaaaaatctc caggtccaca tggagagccg caagctgctc tgcctgacct aagccaagag 71460
catagaaaag gggtcatggg gcacactgtg gtgaagatag gctggcattt gttgcataat 71520
aatttctcca gttgtcttgg gtttctgaga ggtagcttct aaacccttgg aatttcccaa 71580
gtgataggaa tgtctttgtt attcatagtg agccactggg accatgcctg agttcatact 71640
aacaaggtga ctcatggtgg gcccctagat agtttcaaga tggggactag ccattaggaa 71700
ccaaccaaat gattagaggg ttggggtttt gagccagatt atatcagcct gacttccagg 71760
gaggttagag attgagttca atcacatggc caattatttg atcaatcatg cctcgtgatg 71820
aaacaccaat aaaaactctg gacctcaagg ctcaattgag cctcctggtg ggtgaacacc 71880
agtgtgctag gagggtgata catcctgact ccatggggag aagatatgga agctctgtgt 71940
ttgggatcct cccagacctc accctatgga tctcttcatt tggctggtcc tgatttgtac 72000
cctttataat aaaagtgtaa tggtaagtat agcactttgc tgagttatgt gagtcattgt 72060
agcaaattat caaatatgag gggttgtggg aacccccaaa tttgtagcca gttgatcaga 72120
aatgcaggta gcctggggac cccatattat gtggggtctc tgaagtgagg gcagtcttgt 72180
tgaagactat gtccttaacc tgtggagtct gtactaactc caggtagttg acactgcaaa 72240
agtatatact gctgtattgc agagctggat tcatgaatgc ctgtttccag acttggattt 72300
aatttggttg gtggtgggga gccatagaag gtttatgaga agatgaatga caggatcact 72360
aactgagatt ctggaagaat aattgggcag agataaaggc tatctagagc tctccctgag 72420
tcacaggatc ttttggtcat gcactctcaa ctttgacatg tgatgctggt caccaaagat 72480
gcttcctttc aaaaataaac agcagctata aaggaactca catatattgg tagggttgga 72540
gccatcccag ggcaggccag gcctaggagc tacaggaaat cctcacttct ggaagcagaa 72600
ggtcagttgc ttcttcctgg accacaccct ctgcagacac tagggctcat ggaggagtcc 72660
tggagggata cacagcccaa gcctttgctt tccaccgtaa gaggatggca gtagaatctg 72720
ggtacagctt cctcttggag gtttggggaa cccagtgaca ttcaggagcc ccctttttta 72780
aaccttctgt gccctccagc ttgcttcttt ccagcaacag ctagccactg gggcattgta 72840
tttcagcctt tctggggcat cccctgcaag ataggcaccg gcactgcctg ctctctctct 72900
ccctggatct gatttagcct tcactgaaac cctgcccaag atgatgactt aatctctggg 72960
ctgagcgaat gcagccaata tgttcagatg gattttgcaa gacaaaagct caaatctgtt 73020
ttgtggctgc agatacacat tcagactgaa ccacatccgt ggtcattcca gtcacaaaca 73080
caaagggtgc catgaattct ttccatgaaa aataatttcc attgtattgt ttctgtcatc 73140
attcatttca tgataagtca gttaaaacag ccggtccgag tctgtcattg attccaagag 73200
ccccattgtc gtgtccttaa cttactgata gttttaggtc ttaattgtta gtagcaaagt 73260
ttcttttcag gaattctgtt ttctcattga ctttgatgag aaagaaaatg tagagagatg 73320
ctagcgaaaa aatttaattg ccctcaaaga agtagcaatc ttttttcttt aatatatatt 73380
atgcatcaag caaaatcatt ttctgagaac tagttccaga ttgcgtccaa tgtcatgggt 73440
tgtgtgtctt atttatttat tttctcattg agatataatt cacataccgt aaaatttacc 73500
attttaaagt gtacaattca gtggttttag tatattcaaa agactgtaca accatcacca 73560
ctacttccag aacattttca tgacagaaaa aagaaacttc gtcctcatta gcagtcactc 73620
tccacttcca ctgtcttaca ttccctgaca acaactaatc tctcttccat ctctatgtac 73680
ttgcctattc tggacatttc atataagtgg agtcatataa tatgtggcct tttggtctgg 73740
cttctttcac ttagcatgtt ttcaaggttc atccatgtcg tgcgatatat cagtacttca 73800
tttcacttca gaattgcata atactctatt gtatgtgatg cagggcagat gaaccccaaa 73860
ttgggactta gtccatgagg atttttggct ttgcccagga aggaattcaa gggcaagcca 73920
gaggtagaag acagctttat tgaagcagca gtggttacag ctctgtgact gctcctgcag 73980
aacagggcta ccccataggc aaagagtagc agctcagggt attttgcagt catatttata 74040
ccacttttaa ttacatatgg attaaggggt ggtttatgca gaaatttcta gggaaggggt 74100
agtcactttt gggtcagcgg gtcattgcca tggaaaggag tggtaactcc caggtgttgt 74160
catggcaatg gtaaactgac atggctcact ggtgggcata tcttatggaa agctggttct 74220
gccccttcct tgttttagct agtccgcagt ttggtgtctg acttcatctc tggagtcgag 74280
tctcacttcc tacctcatat ggatatacaa taatgtgtat atccattcat caattgatgg 74340
atattggatt gtttccaatt tttggctatt atgaataatg ctgctatgaa cattcatgtg 74400
caagtttttg tactgacata ttttcaaatc tcttgggtat atacctagga gtggaattga 74460
cgtgtcatat agtaaactct atgtttaacc tactgaatta gagtatctgg gactacggcc 74520
cagcaatctg ttttaacagc tctccaggtg attctatgat caccacaatt tgagaggcac 74580
tgccctagag taaggggcag taaacagcta gatagtaaat attggggttt gcaggccaag 74640
agtcaaaact gaggatgtta ggtaagcact tacttgataa gacggtaaac aaatttccaa 74700
acatttttat tgacaaattg aaaaatataa taataataat tggtaacagg gttgtttctg 74760
tttgttggtt tttggttttt tgagacatgg tctcactctg tctcccaggc ttgaatgcat 74820
agctcactgc agccttgacc tcctgggctc aagtgatcct cccaagtaac ttggagctac 74880
aggcatgcac cagcacactg gctacttttt acattttttg taaagatgag gtctccctgt 74940
gtcgcccagg ctggtctcaa acttctgggc tcatgcgatc ctcccacttc agcttcccaa 75000
agtgctggga ttataggcat gagtcactga gtcctgccag gaacagtttt ttgtaatgca 75060
ggtttactaa tgagaagaat gtgtttggtt tttttgtttt tttgtttgtt tgttttttgc 75120
tttgttttgt tttttgcagg attgggaggg caacatttta cttaattggg attcaaagtt 75180
ggtgttccct gtcatcaaat caattgcaga tattcatctg taaaaaccat tcttccggcc 75240
agcgcagtgg ctcatgcctg taatcccagc actttgggag gccgaggcgg gcggatcacc 75300
tgatgcaggg agtctgagac cagcctgacc aacatggaga aaccccgttt ctactaaaaa 75360
tacaaaatta gctgggcgtg gtggtgcatg cctgtaatcc cagctacttg ggagctgagg 75420
caggagaatc atttgaacgc gggaggtgga ggttgcagtg agctgagatt gcgccattgc 75480
actccagcct gggcaacaag agcaaaactc tgtctcaaaa ctacaacacc aacaaaaacc 75540
attcttcctt catgggctgt aaagtaataa gccgtgggcc agatttggcc tgtggctcta 75600
tggtttcaac tagttctggt gcatagcagc ccccaaagtt ttagcagcgt ggttagtgga 75660
gagctgtggc ttttgcctcc atctaaattt ttctttgagc aaattacctc tctcctgaca 75720
gccctgttgg gggtgtcagt tgtggggttc tggtacctcc catcccggcc ccagctagag 75780
caggcaggcc cacaggaatt tgaatctgga gggagttatc agtggtggag cgtagaggct 75840
gcaggaatct tcatataagt ggagtcatat aatatgtggt cttttggtct ggcttcttta 75900
cttagcatgc tttcaaggtt catccatgtc gtgggatata ttagtgcttc atttcacttc 75960
tgatggcagg gtcctgaatt aagtcctgcc ctgaggatca cactgttaca tctaggcctt 76020
gtaggattcc tgaaaattgc atcccatctg ccctgctcta aaatctacac agcctttgcc 76080
atatccattt atcgatttgg ctcagcaaca tctactaatg atgcttaaat cctgggcaag 76140
tgtatttgaa gtggccccag aggttaaaga tctggatgtt ctccctccag caacagcacc 76200
actgccccac agcctgggga ttttctgttc tgcttgctat taatcactta cttattgcat 76260
acttatatgt gccaaaaatg ggatcctctc ttgccctatt gtaaagtgat acccatcttc 76320
tagaaaccaa tccagcaata tatgagaaga gccatagaaa tgtgccccct tggggaaaag 76380
aaataatcct aagtagagaa aaatgtattt gagcaaagtc attcatggcc acattctgcc 76440
cttccgtggc cagaaatgga taaaagctga atctactgcc agcaagtatc acctccactc 76500
attcatgatg cagagcgtcc ccacccttcc cactgggttg ccttccagaa aacagcttcc 76560
atgatgccca gtaatctctg tttcccttga tctctctcat ctccttcctt gtctaacctt 76620
cttctcttag aaaaggagct tcatcccctt tggtcagtag ccagcaaatc tggattgctc 76680
cctgtggccc ttgtgctcaa ggcaaataga accacctgct ccacatcctc ccatcttctg 76740
agtggagggg ccttgagata atccacttct ctgcttagag tggattcttg atgtatcact 76800
ctaatgattt atgaggttaa aagactccct cctcagtagg ggtgagctta aaatgttccc 76860
tcctcagtaa ggatgagcat agagagggaa aaggagctga cagaaccaga gaatcccatc 76920
caccacattt acgatagtga aaacattaga agccatccaa tgttccaaac acctctggta 76980
ccagccaagg gggctgaccc aaggcaggat cccaggagct agggttgagt gggtggggga 77040
ggcactccag gcaacaaggc atcagatctg aggacaggtg ccaggctcag cctgacaagg 77100
taggccaagg gacagttcta gagaagcagc caggataacc tgttttattt tgtttttaat 77160
tgggtgcaga cacaagggga gtatggaaag ccattggctc aggtctcaat gggtctcaaa 77220
tttagacttc tgctattttt ttcttcaagt gcaattgcat atttagtctc agagagtgac 77280
tctgacaaga cccaaagaga acatttgtca tttggccttg accttgtctc cacatctgga 77340
gagttggtat cacgtgtggt accttatttg tacatttctt gggggttcat aacaatgtta 77400
taccacatta ctggtgaaag gaatattaat ttgttcagtg taaacactga aatagctgac 77460
aatcttttat ttttaatttt aatctattta tttgttttga gactgggtta agagattggc 77520
taatatttgt attttgggta gaggtgggat tttaccatgt tgccaaggtt ggtcctgaac 77580
tcttgggctc aagaaatcca ccccccttgg cctcccaaag tgctgggatt acaggtgtga 77640
gccaccacac ccagcctaaa atagctgaca atctttataa ccctctttag gcatatttac 77700
attttatagt atattaggaa cctcaaaaac tgatggcaat tcaggttttt ccagtattca 77760
agaaggcatt atctaagctg tcagatctag gggggtggtg agtgtgatac ataaattggg 77820
aggtaaatca aaaagtctgg agcaggcaga agaatgcagg aagaggtcag ggagtgaagg 77880
actggattga gagggccaag atttggggtt tgaacagggc tgacttcagg gagcgggagt 77940
ctggccccag gtaaaactta gtgtcattgt gtgggtcaga agacaacaaa ggatccttaa 78000
ggaaactatg gcccatcatc tcagtagacc aggcagcctt tgggaatgat ggtcacaaag 78060
actctgtggc aacatagaaa atgctaatgg tgtcatacag aatacagaac tttatggacc 78120
tatggtacaa acaaacacat gcatgtgcct gcacacacac acacacacac acacacacac 78180
atacacagag gaaacaagac tggaaggggt tacacctaaa tataagtagt gcttgtgtcc 78240
atggggtgga gtgatattgt gggtttattt gctctgtatt tttaaataaa acaatggctc 78300
aactcagaag ataactgagt cagcgtctca tccagcagcc cccggggccc ctgggagaag 78360
ccacctccca ccacctggcc aagaagatct agacaggctg agaggctccc tctccctctc 78420
ctggggtccc gagccacaaa tctaaagcca cattccttgg gtgaccaggc agggactggg 78480
gacagatgtc gaaactggca aggccccact ctgtggtacc cagcctgctg tggctcctgt 78540
cctagtgcca gctcctctgc tgtcatcccg tccccacccc acacatcccc acctccccag 78600
ccagttgggc ccaaggtgcc ctcactgata tgttggagga ataatctgct tctctccagc 78660
tcctcaggag acccgcgtgg gaggtcccgc gtcacgggct actgaggcac atgctgagtc 78720
ccctcccatg ggcctcagca cacctactat ggcttagctc ttcccaccca ggacctcctc 78780
agggcttctc ggcattccag aaagacaaga agattttctt ggggccaagg cgttgctggg 78840
cctctctgtc ccggccaggc cctcggcaag ctccatgcct cgactctact gccttctctc 78900
tggtcctctc tgtcaccctt cagagacttt taaatcccat agtctgggct tcagggcctc 78960
caataaaagc agaataagac ctataaggac ttaatagagc ttttatctat aaaagagaca 79020
gaccttctaa tctaactaga aactaaggtt aaaaaaaatg aaaagagtta gacttaaact 79080
cttaaaaaaa taaaaaaaga ttcagctctg tcagagtcag cctgatgtgg tagacaaatt 79140
gctcatgaat tactcacagt gtttgctaaa aaatgctggt tcctgggagt ctctgagcct 79200
actctggctc tagaggctgc ccaatttaaa aaaaagaaaa atgcaggttc ctgagtctag 79260
ctccagaact accaaatcaa aatctcgggg aggtgaggac tgggaatctg aatgtgtaac 79320
acgttcccta cgtgattctg aagcacactg caccggtggt ttagtggttt ccatgccagg 79380
agggcattgc gccctccttg agaaccctgc cttcccagca ctagctgctg ggaggctggc 79440
cagcacagac acagtcctcc ttcccaagag attcctagcc ctggcgagga ggaagactcg 79500
gtgggtaaca ggagttgcaa tgtgggtttg tacccagttc ttggggagca cagaggagag 79560
gggagttctc agagagggaa gatgtctcag ggaatgagtc tctggggcca gatcctgagg 79620
gctcagtagc attgactagc taaagaagag agcagaggta tcccaaattg aggacatagc 79680
atgggcagag gcacaatggg gagctgtaaa cagtttaggg cctgggcaca ggtagggagt 79740
agaggggagg aggccagaga gatgagtggg gctggtcttc ctgctggttt gtgttttgtc 79800
catttggcta atctgggaat aatacttccc agaaacccct tctccagagg ggtctagggt 79860
agagttggtc aaaagaggag cttgcatgag atttggaaaa taaaagtgaa gcagcatcct 79920
gactctcagg gggttgtcac ggtcatgtgc cgtgatgaca ctgaggcaga gatatgcctg 79980
gccaatccac ctctcgggct gctggctccc ggtctggccc gtgttctggc cagctgggct 80040
tgctgagcta cagctgcccg cagacctgtc caccggctcc cttcacggcc tcactcaggc 80100
agctgatgtg cttgcttctt gggcttccct gcaaactctg acttgtcacc cgtattaggc 80160
cattcttgca ttgctataaa gaaatatctg agaccagatg cggtggctca tgcctctgat 80220
cccagcagtt tgggagccca aggcaggagg atcacttaag cctaggaaga ggaagttgca 80280
gtgagccaag attgcaccac tacactccag cctgggcaac agaggaaaac tctgtctcaa 80340
aattaaaaaa tttaaaagaa ggaaggaagg aaggctgact gacctgagtc tgggtaattt 80400
ataaagaaaa gaggtttaat tggcttgtgg tcctgcaggc ggtacaggaa gcatggtgct 80460
gggcatctgc tgggtttctg gggaggcctc aggaagcttc caatcatgtc aaaaggtgaa 80520
gggggaagcc agcacctcac atggagaggg ggagcaaggg agataatggg ggagaggagg 80580
gaagtgctac acgccttttt ttttcttaga cagggtcttg ctctgtcacc caggctgggg 80640
tgcggtggtg caaccacagc tcactgcagc ctcaaactcc caggctcagg tgatcctccc 80700
acctcagtct gcctagtagc tgggactaca ggtgcacacc accattcctg gctaattttt 80760
ttttttttgt attttttgcc atgttgacca ggctggtctt gaactcctgg gctaaagtga 80820
tccacctgac tcagcctcct aaagtattgg aattacaagc atgagccacc atgcccagcc 80880
aggagtccct ttttgttctt tttttaaatt ttattttaga cagagtttca ctcttgttgc 80940
cccggctgga gggtagtgga gcgatctcgg ctcaccgcaa cctctgcctc ctgggttcaa 81000
gcaattctcc tgcctcagcc tcccgagtag ctgggattac aggcatgtgc caccatgccc 81060
ggctaatttt gtatttttat tagagacagg gtttctccat gttggtcagg ctggtctcga 81120
attcctgacc tcaggtgatc cacctgcctc ggcctcccaa actgcttgga ttacaggcat 81180
gagccaccat gcctggcagg agtccctttt taagagcagg aaaaatattt tccataagca 81240
actcctccaa cacacacaca cacacacaca cacacacaca cacacacact cagagtccct 81300
ccatggctca tcggggtaga ccttttaata tccagggctg gagaaggaca atcccttcac 81360
acttgaacgc tgtgaagtct gtgcccttgt ccagggcacc tgccaggagg agtaggcagt 81420
gggtccggtc tttcagtgga gggcttctgc agcttcagat cgtctctagg agtctggagg 81480
tctatcttat ggcttgtatt tctggaaccg agtcatgcct gcttgaagat aagaagatca 81540
aacaacagga tgctgtggct tctcctatcc caggggcctc gaggacaaat cagttccctt 81600
cctgggggag cagatcctcc tggctgggat cttcagtcca agttggttca atttatatcc 81660
ttcacgtctg gcttctcctg catgtccaag tcacaggaat tttaagtcag gatcttctct 81720
gcacccctga gcatgacagc cagcctctgc cctcatgaag ctcccagtct aagggaagaa 81780
ataataaatt ggtggccggg catagtggct catgcctata atcccagcac tttgggaggc 81840
caaggtgggc agatcacttg aggtcgggag ttcgagacca gcctggacaa catggtgaaa 81900
ccccatctct actaacaata caaaaattag ccaggcgtgg tggcatgtgc ctgtagtccc 81960
agctactcag gaggctgagg caggagaatc gctggaacct gggaggcaga ggttgcagtg 82020
agccaagatc atgccactgc cctccagcct gggtgacaga gcaagactct gagattatta 82080
tctcaaaata ataataataa taaaataata ataataaatt ggcagttaga gggcacttca 82140
ccaaggcaat atgagggaaa ctacaatctg tcaggaaaac acaggggaag gccctaactg 82200
gcccaggagg ttcagcgggg catccaggag gatgtatgag agccgaggag ggagtagaag 82260
gtgatgtagg aaaaccactt agctgaaaga ctcgctgccc aaaggccgga ggtgggagag 82320
agtgcgggac ttgcaggaac taagaggagc ccagagaggc atctgagaag agaagggctt 82380
ggagagctgg gagcatcaga tcgggtttag cctgggggcc tggctgaggt gagtgctctc 82440
catcctaagg ccacaggaag cagaggaggg acaggatcag attctgcgcc cagaggaaga 82500
ctggcttaga ggaggtgagt ccagaggcca ggagaatatc agaggccagg acaagggaga 82560
cggcggccaa accagggaag tggcactgga gagaaatgga tggattcaag agacgttagg 82620
caaatggaca gaaaacagaa accgcgcacg tggtggttgg tgagttgaat gcatgcagga 82680
gataggggcc caagggagaa ccataggggg ttgattccaa gggtctggtg gagcccctga 82740
ggggttggag acacccagct ggtcagccac acagtctggt attaaatgac acacggctgg 82800
tcagccacac agaagctggg cagtgtgaat gggtccagta ccgacttcag agtgtgcacc 82860
tctgtctccc cagacagcaa cagaaggacc agggaaattt aaaagggagt ctatgaatga 82920
tattgtaata taaaatctct aatgaagatg tgactactag aaaaaaggga aataaagcga 82980
ttgaggaaag gaaaacaact tgtgcataaa gaagcaaaga ataagcaact gaaagctcaa 83040
aacatttttc caggcctggg ggatgaggtg gagagagtct cttgctccca ctccaggagg 83100
agggctgcct ggaactaaag ttggtgctta tgatctggta tttaaatgac attcttagtg 83160
gtggtgttag cggtagcaga gcaattgtgc cagggcccac cgaccccttg ctggttcact 83220
cagttgccaa catatactga tatactgagc acctgctgca ggccaggctc tgtggtctca 83280
gatctcaaga acaagccagc tcagtccctg tctaatagag cttacaactg ggcagcggag 83340
gcagacctgc catgagacaa atataaaatg acaacttgtg acaagggtta ggaaaaagaa 83400
tagtggaatc ctgttttaga tgggatggtc agggaaggcc tctgaggagg tgatatttga 83460
gccgacatca gatggatggc agagaatgca agaagagtgt ccctggcaga gggaacagca 83520
tcgtcataat tgggagctgg tgctttcgag gaactaacag acaggcaggg tgggtggagg 83580
gtgggagcca gggctgggct gggggttgtg aggacactga caagagacca gttggagagg 83640
gggcgggggc tgaccatgca ggaattggaa tctttcagta aagtccagac caaatgattc 83700
ttcagggtcc ttgttccccc aggtttcaat gacactttaa aggtgttgtc gtaaaaggct 83760
ggggagtctg tttctatgcc cccaatctca tttaagaagg ggggcatcca ggccatcatg 83820
gaacaggtga cctggaagat gttccttggt ccagtttctg caaactgccc cgcagcattt 83880
ttagaaaatg ttccctttta gattcgattt atcttagcca aattgaccag ggaaaatagg 83940
tgcctacaaa tagcgatcac tggcaaacaa ggagagttat tatcttaaat taaggctggt 84000
ttctaacaac aaaaaaaaac ccaccaaatg ccactggcca ccccccacca accccgatcc 84060
ccagcgcata cgtgaggacg atggctccac caccctccct gtaagtacca ggctcaatgc 84120
cgggctctgt gcaaagggaa ggaaacagac agaggaagga aggcaagaga ttagagaagc 84180
tgacagatgt aaatagcctc agaggagcca cactgtcccg gcatttctcc ccagggagcc 84240
ttttgtacca aggaatctgg ttgcctgaaa gaaaaatggt atcatttatt tctttagtca 84300
gagctgagct gttttcttca gacatagaaa taacctaaca tcacacacca aattgttggc 84360
caaatgacag agatacctgt aatgtgggtg tttaataatg tccagggtaa acaatcatgg 84420
acttggtttc ttgggaaggg ccccgttccc cgccatggtt gcaaagctac agagggtctt 84480
gaatgaaata agtgctttgg aaaagttgtc ccaaagttct tgcctgctgc tgagctggga 84540
ggaagctggg cttttcattt ctgctgctca aatcatgccc cagcagccaa acatccatta 84600
attcaacaaa tatttatgga gcccggattc tggccaagcc ctgtgcaaaa gcactgaggc 84660
tacaaatgtg cagaggctgg ggtcctgtcc tcaatagcct catagtccag aggggagacg 84720
gccacgttca gcctgtgtgt caatcttgta acacagcctg gatgtggcgt ggtggtcagc 84780
tctgtggagg aggcagtctc ctcaaaagga gacttgaagg ggaatttcca ggccagagga 84840
gttggaggag gggatcctag acagactaag gagaatgtgc aaaagcagag gcttgaaacc 84900
ccctgggcga tttagaaatt aagagaaata cgatattgca gagaacaaag tgctaaggaa 84960
gtcagcggtc aaatgcgtgg ctggacaggt agatggatac cagatcatgt acttgtaagc 85020
taaggagttt agagttgacc ttttaggtaa taaattccat ggaggaattt gagcggggaa 85080
atagcatgat tagatttttg tattagaaca ctcactcctc tctgtcctga agaaactcta 85140
gcatatgtcg cgtcaggaga caaattcggg catcttcctt agtgcattgg gtttgatggt 85200
gaaaacactg gaaacaaact aaatcagtag aaaaatagat aagcaggcca ggcgcagtgg 85260
ctcacgcctg taatctcagc actttgggag gccgaggcag gtggatcacc tgaggccagg 85320
agttcgagac cagcctggcc aacatggtga aacctcgtct ctactacaaa tacaaaaaat 85380
tagctgggtg tggtggcaca tacctgtagt tccagctact caggaagctg aggcaggaga 85440
attgcttgaa ctcaggaggc agaagttgca gtgagccaac attgcgccat tgcactccag 85500
cctgggtgac agagcgagac tctgtctcaa aaaaaaaaaa aaaagaaaaa tagataagca 85560
aatagtggta tagtcataaa atataatacc attcaagatt ttaaaataat ccaacatggc 85620
tagatcttaa aagcataatt ttgagtaaaa aaaaaaggtg agtttcagca tatgtatggt 85680
atgataaaat gcatgtgaaa tttaaaaacg cacgtttgta tattgattat aaataatata 85740
tgtaggatgg tatgaaaaca tggattgttg cctttggaaa caatccatgg agggaaaggc 85800
atggaaagag aaatacatag aatttttcta ttggttttat atctaaaaga aatatgggcg 85860
gggcatggtg gcttacacct ataatcccag cactttggga ggccaaggca ggaggatcac 85920
ttgagctcag gagtttaaga ccagcctggg aaacatagtg agaccttgtc tctaataaaa 85980
ataaaaaaca ttagctaggt gtagtggtgc acacctgtag tctcagcttc ttgggaggct 86040
gaggtaggaa gaccacttga gcccaggaga tagaggctgc agtgagctat gattgtgcca 86100
ctgtactgca gtctgggcaa cagagtgaaa ccctgtgtaa aaaataaaga aatactgtag 86160
atggagctaa aatgctaacg tattaaattt cctgtgttta aaatattttt tgaactttgc 86220
tttttaaaaa aacagagaaa gtaaaatcca tctggcagcg gcatagcaga tggaagggag 86280
gggattgggg aatgggaccc cgagccagtc tctgtctcca cacagcagct cctgggtgga 86340
ggagagctgg aggccgatct tgggggaccc agggagtaat gcatgcagag aacacttcac 86400
cttgttgggc tgcttttggc ctctgggcag agcggaggcg tggtcctggc atcttgtcta 86460
gtgtccctgg aagatacggg gctctgtctt gatcggaact ggcgaccatt ctgacctggg 86520
atgtcagtat catttttgga catctgccaa ccaagggtca gaaacaggag gattttagaa 86580
tacctgggaa agcttggcat tgtatattca gagtaaagat ggtgtgaatg tgtgtgtgtg 86640
agtgtgtgtg tgcatatgca agcacatgtg caaatccaga agcacagtga aggtgagtgt 86700
tccccaaggg ggtttgcagt ggatgcagtg acgaatacat tcactgatga ggatgctctt 86760
ctatagttca gagaagtaag aatgaatcat gaatctttcc agaaggaatt catttctaga 86820
ctccttctta cctttcagat ctccatcgcc tgacctagag tctcggctga atagcataaa 86880
cctgtatcca tctacatata tttatacatc tggcttagag ccacagcagg gaagaattca 86940
cagcaggatc ttataggcac acagcctata ggatcttata gattacagcc tggatcttat 87000
aggcatccat taaagaaaac tacaggcatt tccgcaactg catatgaatt gtgtcacatc 87060
tgtatgtgct agaaggagtt caagggcatc aatattccta ttgtaataca tctgcccagt 87120
gctagcagca ttacttaagc aaacctatca aaagcctaaa gaataaagat gtggcggggt 87180
gcagtggctc acacctgtaa tcccaacact ttgggaggcc gaggcggggg aatcacgagg 87240
tcaggagatt gagaccatcc tggctaacac agtgaaaccc cgtctctact aaaaatacaa 87300
aaacaaatta gccgggcgtg gtggcacgtg cctgtagtcc cggctactca ggaggctgag 87360
gcaggagaat cacttgaagc caggaggcag aggttgcagt gagccgtgag atcgcaccac 87420
tgcactccag cctgggcgac agagtgagac tctgtctcaa aaaaaaaaaa aaaaaagaat 87480
aaagatgtgc accagcttgg gcaacaaagt gaagccccat ctctaaaaaa atacaaaaat 87540
tagccgggca tggtggcaac tgcctgtagc ccagctactc aggagagtga gacaggagga 87600
tggcttgagc ctgggaggca gaggctgcag tgagccatga ttgctgccac tgcactccag 87660
tctgggtgac aaagcaagac tttgtctcaa aaaaaacatg tatagctaca taattaataa 87720
tatgcaaata ttcttcttta gggtcactta ggttttcaac atacagaccc aagagaaggt 87780
acagagactc attctctctc tctttcaatc acacacacac acacacacac acacacacac 87840
acacccctct tcactataat tataattact atgttggctt ccacatcagg ggttagagcc 87900
ttggcatgga gacgcctgaa aggcacccaa ggcaattagt ggtgtccctt ctccaccccc 87960
tacatacctt caggcccttt gcacttgctg ctccccctcc cagtgcacac acccccagat 88020
ccttgtgtgg ctccctctcc caccacattt tggtcttagc tcaaatgcca cctcctcaga 88080
aatgcctggc ctggccgcct tcagggctgt ctatatgctc accagcttta tttttctttc 88140
gagaactttc cattcccgga cattgtacta aatgtttatt tgttatccgt ccattatcca 88200
tctccttgcc agcactccct gtgagctcca tgaggctcca agattacaac cacatgccca 88260
gagctagggt ggtgcctggc tcacagctgg catccagtca gtagcaatgg aatgaatgaa 88320
tgatcaccct ctcttcctct ccatccccac cctctccttt ttcacttcct ctcccgcccc 88380
atctcccctg cctccgcctt aaatctgctg gcatgaccag ccctcaggaa gttgtcaggc 88440
aggggaggtg tgtgctctgc acccctctcc ttccatctct ctttcccacc tcccttatgg 88500
gtacctatct ctcctacctg cagctgctcg ctgccagctc tggccctctt ggtgggcaga 88560
agcttaggca ccccagacaa ttgtagggaa attgggcatt ggagggcagc cgtaagaaag 88620
gattcagtac ctgccaagga atgatgaatt tagtctctca cccatgaaca ggcatgcact 88680
ttcagggctc aggttcggtc tctaaagcag aacgtcattc agggcaccct tcagtaccca 88740
tgacccagtg tctcgccttt cctgcaggag tcggtgctgc cactatggtg actcagcccc 88800
tctcttctcc tcattcatta cctgggttct tcctgcagtg attgacagta tcctttttct 88860
tttgagatga agtcttgctc tgttgccagg gctggagggc agtggcacaa tcttggctca 88920
ctgcaacctc tgcctcctgg gttcaagcca ttctcctgtc tcagcctccc gagtacctgg 88980
gattacaggt gcatgccacc atgccttgct aatttttgta tttttagtaa agatggggtt 89040
ttgccatgct ggccaggcca gtctctaact cgtgacctca ggtgatccac ccgccttgac 89100
gtcccaaagt actgggatta caggtgtgag ccaccacgcc cagcaagttg cagctcttaa 89160
tagcatgggc accatataat ttactgccca gattgggtca ttttttagag tagaagtggg 89220
agctgttaat aattacgctt ggcctacagg tgtaaacgag accaccctgg gcaaaccagg 89280
cacatggtcg cattgactaa gaggaatatt cccctgcctc ctgcatctcc agccacaaaa 89340
ggagcctcag ctgttggtca aagctgccca atagctgagc ccttgcctgc ccttggacaa 89400
gggagaagac agagggcagg ttccaagaat ggggtgggga tgtggcagga aggacggtgc 89460
aggccccaga gctataatca atgatctggg agccgggttg tggagcacag ggctgtgtca 89520
aggagcaggt gggaggtggg cgggtgtggg acatggcacc agcttttggt gtctgtgact 89580
ctgaacttgg cttactcgca ggtggcttct gcaggctctc caccctccca gctgcgtaag 89640
tcctgcctga ttcaaggaaa acagggaatt ttggggtcct gtggctcctc caacgttctt 89700
ttcctttacc tccttgtgta aatgtggcct gatttgtact tggagcataa gtagacccct 89760
acagagcgtg tgtgtgtgtc tgtgtgtgtg tgtgagagag agagagagtg agagagagag 89820
agagagagag agagaaaggg acctatttcc ttctatccct ctgtctgtct tactctcaga 89880
ctattaatac aagccctgag tctggctgta cccccagaac atgtgccccg ccccctacaa 89940
caaaatgctg cccctcccag ctaggtctgt tgtttgttcc ttttctgatt ggcgccaggc 90000
ttatagaccc catgtaggta gaatataact ttccataaat aacctctaac ccgacctaca 90060
atttagcctt caggtttttt tccccctcgt ggtaatggga ttgcagcctg ggctgatcca 90120
tcctgtatct tcaggtccca gaaagcagac cctaggtttg gacattgctt ggaattcctg 90180
gtacccccat gttgccttgc acatggcaag gactcggtac atgttgagga atggtggatt 90240
ctcttctacc catgagcagc catgcacttg cagtctttgc ttgggctatg ccttctgcct 90300
agaagccccc ttctccacct ggaaaatctc taagcaacac ccactttgtg aagctttccc 90360
taaccactat ccctcatccc ctccacagag caaattactc ctatcttgga ttcccataac 90420
acttggtaca aaacagtgtg acctggttat tgcccatgtg cccaccaggt tctaagcagc 90480
tcaagtgagg ggtagggcaa ggaactgagc ccaagtatgg ctgtgggcaa aacatttaac 90540
cccttggtgc ttcagtctcc tcagattcaa acaccaacct ggggctgggt gtggtggctc 90600
acgcctgtaa tcccagcact ttgggaggcc gaggcaggca gatcacttga ggtcaagagt 90660
tcgagactag cctggccaac atggtgaaac cccgtctcta tcaaaaatac aaaaattagc 90720
caggcgtggt ggcacgcacc tgtaatccca gctactctgg aggctgaggc aggagaatca 90780
cttgaatccg ggaaggcaga agttgcagta agccgagatt gtgacactgc actccagcct 90840
gggcaataga gcgagactct gtctcaaaac aaacaaacaa acaacacaaa cctgacccag 90900
agcccgtgtc cccacccact ggaccgcact gcctctcagg gatggaagat gtctctgttt 90960
cctacctgct ctcatattct gggccctgag acactcatga tgcatggatt ttctgcaggg 91020
gctcactgag gcctcctcgg gggctggagc cccaggaagg gaccggccac tgatacccac 91080
tcacacctat caaagacttg atgatgatga ttaattcaag tgggttattg gtagttaaac 91140
tgacaagtca tcaggaggga cctcagcttt ctttgtgggg gtgggttccc ccaaggtagg 91200
ctggacttaa tttaaagctt tccagttgac catcccacct tagggcaact gaagagtaac 91260
tttagggtta ctcttcatct gaagatgcta actcagctag catccagcac tattaagaca 91320
ccttggggag gcgagacttt gagagaagtg caggaggctg gcccactttt gaagatccta 91380
catttctacc caggaggcat caaatccaca cttcgaagaa gatgaagttt cctgtgagct 91440
ggtcctgaat tgtacttggc tttgtgttgt ctatgccagt actaagcagg acttggccag 91500
gctggtcctc catgaatgac tgtcaaatgg aaaacagaat taggaaccaa agtagtggtg 91560
tagacaaaag gggctggaac ctcagaggag agagggatca tgggagattg gaacatctct 91620
ggaaggttag gggaagagat gacaagtaat gtgaatccta aagaaagagg attagttatt 91680
gggggtgcta agtgtggatg gcaaactaag gaagcaggga tctgcatgac catttggagt 91740
cagtaggaag cttggtggag tgggaggtgg gagtcaagca gagaaggcct cccatgtcag 91800
atagggaaac tgcacttgat cctgggcaag ggggagctat ggaaggcttt tgagcagggg 91860
agtgatacaa tgagaacagc attttggaag ccagcatgga ggggaggctg ctaaaaggat 91920
acaaggctga ggttattacc atgccctggg tgaagaaatg cggtctgggt ttggaaagga 91980
agggataaac atgacttttc aaaggaagac ataattgcca caagggccac cagagagcag 92040
tcaaaggtgt gtccaaggtt ggagctctag tgaccaagag aagggtgtca actttgacag 92100
aaatgacgtc atgtgtggtt gacaaaatga gaccacatc 92139

2

51719

DNA

Homo sapiens

misc_feature

1246, 2572, 2604

n = A,T,C or G

2
ggctaatatt ttttattttt atttttattt atttatttat ttttgagatg gagtctcgct 60
ctgtcaccag gctggagtgc agtggtgcaa tctcggctca ctgcaacctc tgcctcccaa 120
gtttaagtga ttctcttgcc tcagcctccc aagtagctgg gactataggc acgcgccacc 180
acacccagcc aattttttgt acttttaata gagacgaggt ttcactatat tggctagggt 240
ggtcttgaac tcctgacctc aggtgatctg cccgcctcag cctccaaaag tgctggaatt 300
acaggcatga gccaccgcgc ctggccctga cagggctttg taatgctcaa ataatatgat 360
ccaagggtca gagttgagta aactatagaa catagtcctt gcattccacc ccaggggccc 420
aataacgagg tctttctcta aatctcagaa aacactaagc taagggaggt aagttctgca 480
gatgggtttt ctctgtgtgt gtgtgtgtgt gtgtgtatac atatatatat acacacacac 540
atatatatat atacacacat atatatacat ttaaaccttt ggtgctctac ttgtctgcta 600
ccaaaattgt aggcagatga ctgaaaaatg taacacacag aatggcctgc atagaaacct 660
ggctcttgga agtcatatga gtcagtttcc tcaactgtaa agtggtggct cactgtacag 720
acactcagta agcacccagc aaatgaacac cttccagggg ttctgtattg gagtcagctg 780
tatagaatga aaacaaatga caaaagggga aacacacaga gtttattatt ctcccccata 840
aaaaaggccg gaggtgacag tccaagctga gatggcctct cccccaggca ctgtctctct 900
actttgcccc cctgcggcac cttgttcagc tggcatgtca gcattccaca cagcacgaag 960
gaggaaggac aaatggactc tccccatctc tctgcccact gcccccctcc ccacaacccg 1020
tttaaaaaat agatatgggg ggccgggtgc ggtggctcgt gcctgtaacc ccagcacttt 1080
gggaggccga ggcgggtgga tcacgaggtc aggagatcga gaccatcctg gctaacacgg 1140
tgaaaccccg tctccactaa aaatacaaaa aattctccag gcatggtggt ggcaggcgcc 1200
tgtagtctca gctactctgg aggctgaggc aggagaatag cgtganccgg gaggcgtagc 1260
ttgcagcaag gcgagattgc gccactgcac tccaagccta ggcgacagag cgagactcca 1320
tctcaaaaaa aaaaaaaaaa aaaaaataga catgggggcc tggcgcggtg gctcacgccc 1380
gtaatcctag cactttggga gtccgaggtg ggcggatcac gaggtcaggg gttcgagacc 1440
agcctggtca gcgtggtgaa aatccatctc tactaaaaat ataaaaatca gctgggcgtg 1500
gtggcgcatg gctgtagtcc caaccactca ggaggctgag gcaggagaat ctcttgaacc 1560
cgggaggcgg agcttgcagc gagccgagat cgtgccacca cactccagcc tgggcgacag 1620
aggcgagact ccgtctcaaa aaaaaaaaaa aaatagacat agggtcttgc tatgttgccc 1680
aggctggtct taaactcctt ccttgagtga ttctcccacc ttggcttccc aaagtgctgg 1740
gatttacagg agtgagacac agtgcccagc ctctctacat tttttttttt tttttttaag 1800
acagactctc actcagtccc tcaggctgga gtgcagtggt gtgatctcag ctcactgcaa 1860
tctccacctc ccaggttcaa ttgattctcc tgcctcagcc tcccaagtag ctgggactac 1920
acgcctggct aatttttgta tttttagtag agacagggtt ttgtcatgtt ggccaggctg 1980
atctcaaact cctgacctca aatcatccac ccgccttggc ctcccaaagt cctgggatta 2040
caggcatgag ccattgtgcc caaccctccc tgccctgttt taaggagggt cctgttcaac 2100
aattactcta cagaccaatt gcccgcaact atatacttgg cagtgaggaa ctgctgggga 2160
ggctgggaga ttcagcctat aggcaccttg gtggccctaa taactctttt atcttcttct 2220
tcttccccat cagaacctgt tctcaaggaa aagagctaag gtaggctgcc agataaaatg 2280
ctgaggtaag tctgttccag aaaatattgg gacatagact aaaaaattgt tgtttgttta 2340
tctgaaactc aaatttaact gagtgtcttc ttttccccct aaatctggca atcctaatcc 2400
aaggctaaac tcattttcag gacgcaaaag gctctggcct taccttttga gtcaggacgt 2460
tgcactttga cagaaggctc tggaaggaaa ctttaaaggg agccttccag agggaaatgc 2520
ggtgttgggg taggtctgcc tttggctatg ggctttctgg ctgccggagg gncccagggt 2580
cccccaggaa agccttctgt gganggtctt ttgagagaga caaagcagag gggtggagga 2640
agggcggctc aggtggaagg agtgaggaca aaggtgagtg cccctgggca ggaagtgctg 2700
aaagagagaa ggagggaggc caccaggcct gggcctggag ccagcctggg agactcccag 2760
ccgcccactt ctcggggcct cccttttcca gccccttgct ttcgaggcag cagtgccatt 2820
atttggggaa accagctaac cagataggac agcaaaccgg ggatttatgt ggtgtgggaa 2880
cagctcaggt ttccctccct gtttacccag cagtattttt taaaacagaa atcagcgtgt 2940
gggtaaccgc agctgtgagt tactagctct ggctgtgagg gctggggtgg ggggagtctc 3000
ttcagagccc tctgtccact ggcctgggag ctactgaagg aatgtgcctc tccccatccc 3060
aggccaggtg gagaaggtgg ccctgcggaa gttcccagat cactgcccac ctcacccttc 3120
ccctcccgac gaaggccagc acacctgggg gaggtgtgat gatggttcaa ggtctaaagc 3180
tttagagatc agtcagttta ggggtcagaa cccatggagc caggcaagta aacacaggtt 3240
ccccaagcca gctgggaggg agacacctgg gtgcctttga tgggagaaag agggggccaa 3300
cagctacttg gcactggcca attttccctt gcatgaacat gggcccagtg taaccaacca 3360
tatctttcca tttgtcaaaa aaaagccata tttccagatt ttttataggc aacctgtcaa 3420
cttttaaatg ttggcaatga attcaaaaca ctgccattca attaaaagca gcccagaaca 3480
tggcacgact ggttcttgga ataccaatct gcaatctctg atcttgtcca actctctctg 3540
ctccccattt tacagatcag gaagctgagg cccagagagg cctagggact tagccatggc 3600
tgcagcagtt gttattggga atgccatgag gccaggatgc ctgttatgtg ttctttgccc 3660
agcctagcag tttggctggc ttggctatgc cagaggtctc accatgcagt tctcaagtgc 3720
ttcctgagca tctctcattt gcatagacat gacatttaca aacacatcca tcatctactt 3780
gattctggca atgacaccga gggagcagaa ctggcattaa tccatttttt ttaatggtga 3840
gaacactgag gtttggagag gctggtgacc tgccaaagtc acagtcctag aaagggctgc 3900
agccagggcc tccaattaca gatcaagggt ttttccaccc ccaaactcag tcatctcgta 3960
aaaacgtcag gctccttggg gaatacagct tgagatggat aatcaactcc tcctaaaagg 4020
gaaccataaa tggatgagat acttgggtaa actgaatatt ctttgtaact taaagttaat 4080
tgaaaatcca taggttatca attttccaag caggagaata aaatagaagg tatttcaaag 4140
agcaattata gtaggcatgg cttaatcttt ctttgatgat tctgaaggca tttcagggga 4200
atgtaatgcc ttagggcatc attatgaaca ccaataatta ctgtgtaggg ctgtgacgca 4260
aattctcatc aatcccctcc tggctgtcgg ggtgttttca gagaggatca tgcagggagt 4320
gtgaacgtgt gcctgtctgt ttcttgctgc tctctccgcc tttgtcaatt tcagggtgct 4380
gttgtggaaa atgcagtgtc cctggaaggg ggaggtcact taaccttaat gagcttctgt 4440
ttctttatta aaatggggta atgcagactc ctatactcac tgtcaaggtt ttgtggggct 4500
cagatgtcag tgtcagagca ggaagaaact ttagatcatg atatgcacca gcttcattct 4560
gccatggaaa aatctgaggc ccagagaggt taggagcctc gtccaaggtc acccagccag 4620
ggagagggtg ctagaacctg ggttttctgc ttcctaacct ctttcctgaa tataacacta 4680
tggaagaaaa agatctggag gaacggaaag atgaacacat gattacacat gcaaacaata 4740
ctggggtctc tctgatcaga gaaggtgtga tcttccagcc tcaggagagg ggccatccaa 4800
gtcctggagg aggtgaaccc cttagttcag ggctgggagt agcctggggg cagcagagac 4860
actgctgtga ggtttatagt ttcatgactg tcagagcttt ttaaaatgtg gtaattttaa 4920
gtgtgcagcc tcccagggtc tttcttcttt taattgaaga aataaaccat ctcccctaag 4980
gcatgcttgg cgaaggagaa aggcaggtgc aaggctcaca gaggagagca gcagcctaga 5040
agggctctgt gtcatgggga agtaaaacat cccagaaaca gagagcagaa ggccttgact 5100
gagccccagg agaggcagga caccaggggt gcacacccat aaacacacac atacacatgt 5160
atgtctcctc cctggagcct gagagtccct atatacagca ggtgcatgtg ggccacacat 5220
cacacaaaat tgaatacagg caggctcaga gcaccagcac acacgtatgt ccttgacacc 5280
cttagagata ctactaagca cgtgtgtgta cctgctcacc catatggcag agcccctgga 5340
tctgggcaga aatgccaaag caggggcagg cgcgtgtgcg cgcacacaca cacacacaca 5400
cacacacaca cactagcaca gccacaaaag ctcaatccac atccagcatt cctaacaaca 5460
cacacacagc tagacacgct aggagacaca tcaggacaat gtttccactc ccgctgccat 5520
acacatatgc aggtccacat tcaccagtgg ggtagggtag agtctcatca gtccagactc 5580
gcagacagct ggacacagag gtgatctctg aaacccaatg tctacacact gtggtctttg 5640
ttacacacac acacacacac attgaaatga tgtcctcagc ctttggttat ttttggtttc 5700
ttctgagctg gagtttcact cttgttgcct aggctagagt gcagtggcgt gatctcagct 5760
cactgcaacc tccacctccc gggttcaagt gattctcctg cctcagcctc ccaagtggtt 5820
gggactacag gcgcccgcca ccacacccta ctaatttttg tatttttagt ggaaacaggg 5880
tttcaccatg ttggccaggc tggccttgaa ctcctgacct cagatgatcc ttccgccttg 5940
gcctcccaaa gtgctgggat tacaagcttg agccaccgca cccggccgag ccttcgggta 6000
ttttgaaagc tgaatgtgtg gttacatttt cttttctttt tcttcttttt tttttgagac 6060
ggagtctctc tctgtcacca ggctggagtg cagtggcgcg atcctggctc actgcaacct 6120
ctgactcccc ggttcaagtg attctcctgt ctcagcctcc tgagtagcta ggattacagg 6180
catgcaccac cacgtccagc taatttttgt atttttagta gagccagggt ttcaccatgt 6240
tggccaggat ggtctccatc tcctcaaatc gtcatccgcc cgccttggcc tcccgaagtg 6300
ctgggattac aggcgtgagc cacagtgccc ggcctacgtt ttcaaacagc aatagcattc 6360
gcctcctctg tcagtttaac ccccatcaca actcccactt ttggcaccta aacagttaat 6420
ttcccagttc atgggccttc aaagtcctgc tctagtctct ggaggaactt tcacctacag 6480
aggaaggtgt aagggaaact agttcatgga tttaagtaga aacattttag gtgtagcttt 6540
cacatacaga ggagtgagaa aaaactgatt catggattta gatggaaaca ttgtagtatg 6600
aacccagcgg agggtctggg agcgccttct ggtggtgaga attagaaccg cagcactttc 6660
tgcaatgtgc ccaggccaga aagctctacc ttctgatagg acccacttct gaccctagaa 6720
tgggggaact gatggaggtg tcaagccact gtggtcccac agctgcatgc aggcacaggg 6780
gataggaaga gagctaccta caggttacta aaccattccc ttttaaaaca gcaccaggct 6840
tatgtctact ctgcgcttcc attttctagg ttttaagtgg aagatatgtg aacacccagt 6900
gggctggatg gctgtccctg ctacaagtct gtgatgtctc cgtccagtgg cagagctgga 6960
aggcaggtgc tgtcggggct gcatctgcct tgttcaccag cataggccta aaaccatgga 7020
gggggtgctt tggcttagat ccccacttgg cctgtgtgtg taagaggctc tcaggcacct 7080
taatgctaca tcaccaacca aacctcctga tgattctttt aggttctccg tttccaggca 7140
gattcacttc tgtagattta tttatttatt tttgagacag ggtctggctc tgtcccgcag 7200
gctggagtac agtgatgcaa tctcggctca ctgtagcctt gacctcaact tcaagtgatc 7260
ctccaacctc agcctcccag tatgagggac cacaggtgtg caccatcaca cctggctaat 7320
ttttgtactt tttgtagaga cggggtctca ccatgttgcc cagctggtct caaactccta 7380
ggctcaagcg atccaccaac cttggcctct gaaatgctgg gattccaggt gtgaagcacc 7440
gcgcccagtc cctgacttct gtagacgttt gtattgttta catctactgt gtgcaatgta 7500
cgagatgcag tcaggtgtct ggatggacca ggggatctgg catcttatag acttgggttt 7560
gaaccccaac tgagccattt actggctggg tgactttggg tgagttctta aacctctctg 7620
acccttaagg tggtaacagt atcaccagtg aagttggtgc acacagcagg gcccaaactg 7680
acatctcaca gcacccagca ggtcactgtg ggcatgatag gatgatgggt cactgtgcca 7740
gccctgaagg agttcaagtc cagatagggg aaggtggtgg accagaccca gacagagatt 7800
ctgagtcgct gctgagactg ggtgagggta gtgggtacat gggaggacat atagcccggc 7860
agcccagggc tggagtccac actcaggttg gggcagcctg gtctgcctct cctgcaggag 7920
acttttccag gcaggcttgt ccctccagaa tgcacgaatc aaatcctctc aggatcagtc 7980
tcattttcct cgtgctgggg gagcaggcta ctcacagaag atgttgttgc aaatgtaaga 8040
atcacatgtc gatccacaaa ctggcattga gcagctacct aggagatcaa agaaactctt 8100
actttgggag ctcctgccag ggctctttgg gaggtctggc tagctctgga ggaagagaat 8160
gaacttgggg agggcgtgga acagatgagg acgcaggcac tgccattcaa agaggagagg 8220
tctcccggac agggctggcc tgggcaggcc cagggagggt ggggctggag cagggacttg 8280
aaaaagggag agggctcagg agactcagag gaggaggaaa gtgtgtgagc agtaggcagg 8340
gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgcac gcgcgcgcat gcaggcctgt 8400
gtagggctgg gaaagaacaa agcaaaaggg tgcacaaggc atccagaagc cagggcaatg 8460
cagaacagga acaaagcagt ttggctcaag gggaaagttc tgatggaaag caaggaaaga 8520
gaatgttaga agggctgaga gccagaatgc ccagtatggg gtacagaggg caacagaggg 8580
gctgatgttg gtggcgaggt ggggaggagc atttcacagg gaactgggca gctccaagga 8640
tgggctggag gagggacggt cctaaagggg gtggtgggag acaggcagtt agtgatgggg 8700
ggagagaaat gctctgaggg gggctgctgt aggcagggag gggcaggtag caggaggcag 8760
agggttgagc ctgcagaagc ccggagcagt ctgtagcttt ctaatgcttc ctagggaatt 8820
ctgctgctga gtagccatgg gttcaacagg tctctaggat ttgccttgaa ttactgccct 8880
gaggccccct aagtctgggg tctcataaat tcagcgggga gggcacacct gtgtctgagt 8940
ctgagcagcc cagagccccc agtgttgtca gtgaacagct cactgaactt gcagagcaat 9000
taaatcccag ggcagaagaa agagcaggag ggcagggtgg cccccagctc cctcccatcc 9060
cgggtttggg gagagacacc tctggcctga gactcctcgt ggcctctaac aagtcttgct 9120
ctctctctct tttttttttt tttttaaatt attgtctccc gccctccttc cttcttcttg 9180
ggaatcagaa aagaaacctc aatgcctggc tttgcccctc cctggctgtg gtcaagccat 9240
gtgacattta gacatttcag agcctcctga ctcctgaggc tggagacagc atgaagccaa 9300
atgggcttgg caaacagaag agaggggcag ttaacattct ctgcaatgct ctcttacttc 9360
ccgtttaaac acccctccta ctcggacaca gacccccaac ccctgtgcca ttgtgctcac 9420
tcccaccttg gaggggtcag gctgtggtga aaggtcaagg tgaaaagtca tgatgatcct 9480
attgctgcca cctccctggg gtgagcaaga ttctgtggtg gctccagctc tcccatggca 9540
gtccacacag aggctgttcc aggttctgga gaggtcactt ggtgtgacct gggaatctgc 9600
atcttttctc tgccaaagca gagggcattg cagcaaccac acctgggtcc cagtcctcaa 9660
caaagtccct ccagctcccg ctgctgtctg caaggtttgg tcatctgtcc ctgcattgga 9720
gaagagtttg cactgtgaat cttgcctcca ccagggaagg ctggtaaaag tccctgcccc 9780
gggggccctc attgctccag ggtgcatctg ggggccagac ctgtggaccc tcacccttga 9840
gcctcatttt gttcctctta gaacaaagcc atttctggta aataggtttg atgggtttgg 9900
cagcagggaa ggcacagaac ctttcacgat tagcaggctc atgacaattt ctctttaggc 9960
aagagagaag gttgaggagg aaagactggc agggcttgga gagctggaaa ggaagaaagg 10020
ccctacaggc ctggggatcc catctctgac caccgacccc agcccccaac tctcatagac 10080
tctgatttga tattcttatt taccaaagaa gctgcatgta tttgcatatc aattctcttt 10140
cccatatcga cacgcaggga ggcttcttcg ttttggatca accagagatc accaatgtct 10200
gcagggtgct cagcccctgg taccttggtc actctaggca gcctcctaga tgtgcctctt 10260
aagataggtt ttctgaaatg ggagaggacc acgccccgcc ccccaccatg gactctggag 10320
tctgcggcgc acaaatcctg cgattttaca gatggggaaa cagaggcacg gagaaagtct 10380
cagctttccc acggcccact gggaccagaa cccaggtcgc aggacgggga cagccacctc 10440
ctttttctct ccttgcaacc agcctgagct cgctcagcgg gtgggggctg ccgctatcca 10500
gagcaccctg tttctggatg caaaataaag gcccaggcag tgtttggccc tcctctgccc 10560
acagactttg gctccagggc agtctccgag agagaagcct tgggtcccac tggtccgagc 10620
tctgcgtgct gagtctagag gctgcagtct ctaagccgaa actagcccag gcctcacagc 10680
cgccttcccg ccggtctccc actgccttgg cggggcgctg gcgccctctg ctggccaagt 10740
ctcgctctgc gcgcaaacgc cccagaaatc agccggaagt tccctggggc cccacaggag 10800
tgaggaccca cctggattca ctttgtcatt tttttttagc tgcatgacct taagaaagct 10860
aatgaacctc agagcactac ctgtgaagtg gaaataatag cgcctacact tcagggtcgg 10920
tgtaaggatt gcatagaata ccgtatagaa agcacccagc acattgtagg tatgtttaat 10980
ggtagcgatt attaataatg cccaactcac atggtgagat gagatctgag aagcctcctt 11040
ataaaggtaa atgataacca agtcttgttg caaggcagct ggtgtgttac taatacatca 11100
tgaggtggtg ggccctctgg ggactgtcag gagacagcct ctgttttatt aggaaataaa 11160
acagaatctg gtctgctcca gttttcttcc ttttatttac tgcattagaa aactttcatt 11220
ttatttattg cctattctgg gggttgaggg tgaggggaag gcatgactcc agccaggtag 11280
gatacaaaag tatctcaccc cccaggaggg gtttgaggac cggccctccg gcttttagag 11340
aagggcagct ggcttctgtt gctaacgatt tggatttggc ttttaacaga atatcaaggt 11400
gaaagtgaaa ggagcttttc actccttgga gagtcaagag ggtgtggggt gggacctcca 11460
ggggaatgaa gagtgagcga gggtgctggg gggatcccag acctaggaat cagatctggg 11520
gaaggggtct gcaagacccc tccatgagtc aagaaaagcc ggtgggtgga tggtgagaag 11580
gaccagtgaa gacactgttt gctggagttg tcccagccag tggctatgac tgagactgtc 11640
ccatggcgtg tgcccagggt cctgccattg gatgatggga cattcttttt tttttttttt 11700
tttttgagac ggagtctcgc tctgtcatcc agagctggag tgcagtggcg caatatcaaa 11760
gtcgctgcag cctccacctc ccgggttcaa gcaattctcc tgcctcagcc tcccaagtag 11820
ctgggattac aggcgtgggc caccacatct ggctaatttt tgtattttta gtagagatgg 11880
agtttctact ccatcaggct ggtctccaac ttctggcctc aagtgatctg ctgcctcggc 11940
ctcccaaagt gctgggatta cagacgtgag tcactgtgcc tggcctggat ggaacattct 12000
gctctttctc catctgctgc ctacccatga tgtcttggga ctcctagaac cctaaaggaa 12060
gcccctggac acgcaggaag gtgtggagag gagttctcat acttgcactt gggaggaggg 12120
ctcaggagaa acagaggctg gcaacacccc ctcacacact ggtcctctgg agggccagtg 12180
tctacagaca ctgtggactg agtccacaga gaggaaaggg tcctgccttc atcagaactg 12240
ctcagcaagc agttccatcc cagggggtcc tgcgaggtaa gggaggggca gctagctagg 12300
tgaggggctg agagagtggg gaggggaaag agggaagaag agagtgagag ggagagggag 12360
ggactgagcc gattctcagc tccttgaccg tttgctgagc tctgtctgag tggacagatg 12420
gtcccaagtc aggccacacc agagtggcct ttctgctccc ctacaccctg cattcctcaa 12480
cattgctggc cccggagaga ctttccttca gagaagcaaa tggctgggga atggtgaaag 12540
acactgcaga gaaaagaaag cacagcctgc tgccctggga attaacatga tttaggagac 12600
ctgcaggtca ccccctcatg actaaaagcc atcctggaat gaaggtctgt ggctatttct 12660
aggcaaaact gtctgataag ataaaatagc tcaactcctg accattaagt cgtgaaggcc 12720
atggccatcg taaatctcat ctttccggcc ctctggcctg catgcagtgc agcccagcca 12780
gtcggtggca gccaccttgg taggaagggc cctcatcctc ctggctgtgc cccaaggact 12840
gggcaggctt cggtgccaag ggtagtgcga gcacttgaaa gccgccctgt atgtttattg 12900
ttttccccag gtgatccaga attactcccg aactctacca gctgaaatcc tcctcaactc 12960
acatcagaca agacggccct gccacttacc tgtcagatca ctttgggcag gtaagctcat 13020
tttcctgaat ctttacttcc acaccttaaa atgtgagcaa tactatctcc ctggcaaggt 13080
tgtttgtgag ggtaaaatga aacaataatc acgggtgcat cctggagctc tttcttacaa 13140
ggcgtgcccc caaatctgtc ccctctttct gaggatgccc ttcccctatt gtctccctgg 13200
ccatttccta cccattctca agggccatga tctcagggag ttctcctgac tcacccaggc 13260
atattggatc tcctacgtac tgctacgact gcacacaggt gcaggaaatg gctgtttgct 13320
ttgcgtttga ggaacttgga aagggagacg tggtagggaa agtagtggtt aggggcactt 13380
tcagactgaa ggatgtgggt tggggaatac gggattcttg gagttgagga cgccgcttct 13440
ctcgtctcta gctaatgtga gaaagaccct tctggacact aagcctgcaa ttccactggt 13500
ggctaccagg tgtccgtggt gtcctggggc gggtgtaatg agagcgggag cctgtgaaac 13560
caaaagcatt gtttttataa attcagcact cttcaatccc tattaataag gttagcggtg 13620
cagttcttgc gtctcctgcc ctgcctcacc ttgcaatcat attcattggc attcctttct 13680
tccaagaacc cacctaggag gccttgcagg agatatctct ggtgctggct gttcctgcag 13740
tctgaaaagc cagttaagat acaaatatgt gagaggacac tgcttgaatc tgatttcatt 13800
ttttaaaaag tttaattatt cacagacttt gcttctttct gactagtaat gtgacacctg 13860
tgactcagtt caagatggtg tgtggtgacc ttgcagttga gaagcactga gcgctatagc 13920
catgccaaga aggttgccct cctgggacca gcaaaaattc catccagggg gccatctctg 13980
tcaggcttgt cctgactatc tttttggaga ggcggcagag tggagtggtt aaaagcatga 14040
ttctggagcc cagctacctg ggagcaaagc tcgtctctac cgcttaccag ctctgagagc 14100
ttaggcaagt gacctattct ctctgtgcct ctgagttttc atctgtgaaa tgggagtaac 14160
aatagtcctg tctcacaggg ttgctgggag gcttgaatga gttaatgtcc atggggtgct 14220
gaatcagtgc ctggcatacg gtgaaggcca tgtgagcagt aaatattatt attattagaa 14280
aggttggctg ggcgtggtgg ctcacacctg taatcccagc actttgggag gctgaggtgg 14340
gcagattacg aggtcaagag ttagagacca gcctggccaa catggtgaaa ccccatctct 14400
actaaaaata caaaagacat tagccggtat agtggtgcgt gcttgtagtc ccagctactc 14460
aggaggctaa ggcagaagga tcgcttgaac ccgggaggca gaggtttcag tgagctgaga 14520
tcacgccact gcactccagc ctgggcaaca gagtgagact ccatctcaaa aaaaaaaaaa 14580
aaaagaaaga aagaaagaaa aaaagaaaag gtcccaacct actcacttat tgttcacttg 14640
catcaaatgc taggttcagt gtattgttct tgtaagatta gaaatggaga gatgggtcag 14700
aaggggttgg tcaaaataga cctctcaacc caagcagaca gggctggctt catggccggg 14760
caaccatgca ggacccaggg ctctaagata agaaaggcac gtagtttaat gctttgctgt 14820
cccatcttga tactgttttt tttttctttt ttttttggaa atggagtttc actattgtca 14880
cccaagataa agtacaatgg cgtgatctca gctcactgca atctctgcct cctgggttca 14940
agcaattctc ctgcttcagc tccttgagta gctgggatta caggcatgta ccaccacact 15000
cagctaattt ttgtattttt agtagagacg gggtttcacc atgttggcca ggctggctgg 15060
tctcgaactc ctgatcgcag gtgatccacc cacctcggcc ccacaaagtg ctgggattac 15120
aggcatgaac caccgtgcct agctgatatt cttaatttat gaaggaaggg ccccaaattt 15180
tcattttgca ctgggcccac aaataacgta gcaggtccca caaacaaatt cgtctagatt 15240
cagagggccc cttgccttcc tcctctgctc acattcgttc ctttctccca tcacaggcgg 15300
gtaccctacc ttgggggatt tgccccagaa taagcctttt ttttcccttc taacatttta 15360
atgaaaaatt tcaagtgtat agcaatgttg aaaagatttt atagtgagca cccatacacc 15420
tgccacccaa agtctaccat taatgtcttc tggacttact tttgccgtct tattcatact 15480
ctgcctggac tgtcctcagc tacaggactc acatctcttg ccgacagctc taaggcttcc 15540
agtcctgctg tctggaccaa gaaaggcttc ctgggctctg agtgtcaaat ggcggccttc 15600
aaggaagggg aatggtggaa aaggccgtgg ggggttttgg agaaattgct agggaaagac 15660
tggcaccaga gttccaccag cccaggcaat ggggggtaca gaacccataa gatgagttct 15720
agaaaagcaa ggaaggtttc gttctggagt ttgtggactg aggtttccat ttgtgactag 15780
gattctcatt ggttccttat gtagtttctt acgccctgca tagtcttcta agcatttctc 15840
acagacgtgg tctgggggcg aacaccagca gccctgagag gtggctaaag aggagattct 15900
tctctccact tcacatcgta ggaaactgag tctcagaggt tccttccctg gcctgcccac 15960
aaccccaggg ctaaaagagg cagaccaagc ccagggcctt gaaccccaac aatgggcctc 16020
tttcttttga tcccatgata ggggtgcaaa agcattgcat tcccctgggt aatttgaaga 16080
aaaaacccaa aaaactccaa ctttgtctcc aggaaaaaga gggtgtctgg gctatgattt 16140
acctctgagg gtgtggttgc actgagcgtg atcacacttc aaagggttag atctcatttc 16200
tctgcctttc tagcttgggc ccagggctca gaaatgtgtg gactccctca cagcccctcc 16260
cagcatccct gccccctccc aactgccttg ggcaggtgac acctgtatta ttgctaaggg 16320
ttaaaaagcc cccaaatcaa taaaacccat taatgagtgt tggtacctcg aaggctacag 16380
ataaatccct tctactcagt gagttcaatc ccataaaaca gctctcccct ttcaatccta 16440
gcattcattt gatagaaaat gtggagaaat tttaaaaagg tgacttacta attgcctgta 16500
aaataaaagg cagatggaag ctttattaca gttgaaggaa gtcgggaata ttaaggtaaa 16560
atgtcaaata acaattgatt ttccttagac ataaaggggc gatttatggc ttcctagtta 16620
ctacaaacga gaaattattt gaagttctga aaagtatgag gagaaataaa gattaaatag 16680
aagatgaaat catagggatt tctctgggag gtgacttcag tgcccctggg gactagaatt 16740
catgtggcca gtggcctagc cagctggggc ttggcagttt caagatttag aggcaaggtg 16800
tctctgagga gcggggaagt ggctgtttgc tttgtgtctg aggaactagg aaagaaagat 16860
gaagataggg aaagttgtgg ttagggccaa tttcagactg agggatgtgg gattagggga 16920
ttcttgtgga tgggcctata gctctgcttc ctgactagca gatactgggg atctggggaa 16980
gggaagggtg agctgccttt cctgggactt cgatggcatc cttacagtca ggggacactg 17040
tccttgctgg gtcccggaca tcagtgtcta tgtatccctg caagccacag cactatccag 17100
gccctctggt ggctttgctt ggtctgggcc aacctggtct ccacactgac agtcaaagac 17160
gaggagagga aaaagaactc acggctaggc gtggtggctc ctgcctgtaa ttccagcact 17220
ttgagaggcc gaggcgggtg gatcccttga gcccaggagt ttgagaccag cctgggcaac 17280
atggcgaaat ccccgtctct acaaaaaata gagaacaatt acccaggaat ggtggtactc 17340
tggaggttga ggtgggaaga tggcttgagc tcgggaggtt gaggctacag tgagctgtga 17400
ttgtgccacc gcactccagc atgggcgaca gagcaagaca ctgtctcaaa aaaaaggctc 17460
atttccaggc tgccaggctt atgttagcct ggggtcccgc aggagctgac ctgagacaag 17520
tacttgaggg caagttgttt gtttgaggga tgctcacagg aagctccagt aggagggtag 17580
ggaggtgacc cagggaagga aggcagctta caggggcgtg ttgtcaggaa gggcaccccg 17640
gcgggtgacg gaagcttaat tttgctggga aactcaggag ccagcatgga acctgcacct 17700
cagagttatc ccactggaag gatgagggag cgggtactta tacaccaact ccatccccgt 17760
cctttgttaa ggctgctgga tggagtgggt gggacactca ttcttcagca ctcccagggg 17820
ccctcaggct gtcagaagtt aggtccgttg ggctccagga tgaggggacc cccagaagat 17880
gcgggagggc gtccgcagca tctgcctctg tgctttccct gtttatctga atcctcataa 17940
ttcccgccac atgcagatca gagcccccag ctttatggaa gagaacacag gtttggagaa 18000
gataaagagc attcccacaa ctaggaggtg gggaaagcta ggagttcagc ccagagctcc 18060
ctgacttcaa agtccattct ctttctactt cctgattttt tttttttttt tttttttttg 18120
agacggagtt tcactctgtt gtctaggctg gggtgcagtg gcacaaactt ggctcactgc 18180
agtctctgcc tcctgggttc aagcaattct cctgcctcag cctctcgagt agctgggact 18240
acaggcgccc gctaccatgc ctggctaatt ttttgtgttt ttaatagaga cagggtttca 18300
ccgtgttggc caggctggtc tcgaactcct gacctcaggt gatccgcttg ccttggcctc 18360
ccaaagtgct gggattacaa gcacgagcca ctgtgctcgg cctacttcct gatttttgta 18420
taagacacat cccagcagca tggtagactg aagactcctg ggcccactct cagagattct 18480
gctctggcaa ggatgtgttt attggtgaga ggggtgtcca ggaatacgtg cctttttttt 18540
tttttctaga gacagggtct cgttgtccag gctggagtgc agtggtgcaa tcatagctta 18600
ctgcaatctc aaacttctgg gttcgagcga tcctcctatc tcagcctcct aagtagctgg 18660
gactacaggt gcacgccacc atgtctggct aatttttaaa ttttttctgg agttggggtc 18720
ttgtgatgtt gcccaggctg atctttaact tcaggtctca agagatcctc ccaccttggc 18780
ctcccaaatt tttgggatta cagccatgag ccactatgcc caactagaat atgcactttt 18840
ttttttttga gaccgagttt tgctcttgtt gcccaggctg gagtataatg gtgcgatctc 18900
ggctcaccgc aacctccccc tcccaggtcc aagcgattct tctgcctcag cctcctgagg 18960
agctgggatt acaggcatgt gccaccatac caggctaatt ttgtattttt agtagagatg 19020
gggtttcttc gtgttggtca ggctggtctt gaactcctga cctcaggtga tcagccccgc 19080
ctgctttggc ctcccaaagt gctaggatta caggcatgag ccaccaagcc tgaccttttt 19140
tttttttttt gagacagagt cttgctttgt cacccaggct ggagtgcagt ggcacgatct 19200
cggctcactg caagctctgc ctcccaggtt catgccattc tcctgcctca ggctcccaag 19260
tagctgggac tacaggcact tgccaccacg cccggctaat tttttgtatt tttagtagag 19320
atggggtttc accgtgttag ccaggatggt cttgatttcc tgacctcgtg atccacctgc 19380
cttggcctcc caaagtgctg ggattatagg cgtgagccac cgtgcccggc cttttttttt 19440
tttttttttt tttttgagac aggatcttgc ttggttgccc aggctggagt gcagtggcct 19500
gattacagct cactgcagcc tcaatctcct ggactcaagc aatcctctca cctcagcctc 19560
ttgagtagct gggaccacag gtgtgtgcta ccacacccag ataatttttg tgctttttgt 19620
agagacaggg tttcgacatg ttgcccaggc cggactaaaa ttcctgggct caagtgatcc 19680
tcctgcctca gcttcccaaa gtgctgggat tacaggtatg tgccatcgtc accagccaga 19740
atatgcattt tctttttttc agacggggtc tcactctgtt gcccaggctg gagtgcagtg 19800
gtgtgatctc agctcactgc aacctctgcc ttctgagttc aagcgattct cctgcctcag 19860
cctcccaagc agctaggatt acaggtgtct gccacaacac ccggctaatt tttgcatttt 19920
tagtaaagat ggggtttcac catgttggtc aggctgatca caaactcccg actgcaagtg 19980
atccgcttac ctcggcctcc caaagtgtta ggattacagg catgagccat tgtgcctggc 20040
cgaatatgca cttttaataa gcatcagcca ggctaggcag gccgggggcc acactcgaga 20100
acatttgcac cacagccact ggctacctgc ccctttttcc ataaggttcc actgccctct 20160
ctcccctcta tctgggtctg ttcttcaggt tcttccctgg gagctctctg agtgacataa 20220
ctgtccccaa gtgctgggag atggagagag gaatcaccag actggagcag gcccccagag 20280
cggagatggg aaggggaggc tggtgttctg aggctcccga ggcagtgaga ggtgaccgga 20340
ggcagtgaga ggtgaccgaa gacagtggct gagaaccagg gaggggctgc gggaaaaagc 20400
cctgggtgca agtcgctctt tccttagcgt ctttgagagg agggatgggg aaaggtgagg 20460
tactagggaa aaccatctgg aaggaggtca ggctgcagaa aagctgcagg agtctggggg 20520
actaagaaaa cagtgggaga ccccgctgca gcccagcacg tgagggtgag aacgtcatga 20580
atgagggaag agcaggcagg gggtggtggc cttgtggcct ctggagagga ggagccacat 20640
gactctgggg tgatctgggg tgaccctcag ggtaagggta cctccctagc actgagtaca 20700
gagggaagcc tacactgcca ggtgcagttt ccctggcaat gcttctcctt ctaacactat 20760
gtgagtttcc tagtgctgct gtaacaaact gccataaacc aagtggcttc agagaacaca 20820
gacggactat gacagttgtg gagggaagaa gtttgaaaag cggggtgccc gcaggctgca 20880
gtgcttctgc aggctccagg gagaatctgg tccttgcctc ttccagcctc tagagcctgc 20940
cgcactcctt ggctcatggc cccgtgtcat tgtagcctct gcttctgcca tcacatctcc 21000
tcctctgcct ctcctgcctc cctctgtcct ttataagaac gcttttgagt atattggacc 21060
catctggata gtccaggata aatccctcca tcctcatatc cttaatttaa tcacacctgg 21120
aaggttccct ttgccacata aggtaacata ttcacattgg gacagttagg ggattgggat 21180
gtggacatct tttgaggtgg gaagaaggag ctggggttct ttttaacatt tttttgagac 21240
agggtctctg tcacctaggc tggagtgcag tggtgtgatt gagactcact gcagcagcct 21300
caacctcctg ggctcaagtg atcctcccac ctcagcttcc tgagtagctg ggactacagg 21360
tgtgcaccac catgcccaga tgatttttgt attattttaa tattttgtac acatggaggt 21420
cttttaatgc tgcccaggct ggactcaaac tcctgggctc aagcaatcct cccgtcttag 21480
cctcccaaag tgctgggatt acaggtgtgt gccaccagac ccagcccgga cctggggttc 21540
ctgtctcatg tcagccttag caatctgggt gaccctgggc aaggcctatc ccctctctgg 21600
gcctttgttt ctccacctgt gcaatgaggc tgttccctct ggctccttca ctctgagttt 21660
ttcagttggg agaatatctt ggcagggagc agaggtcggc gggggtggtt gtcattccat 21720
ttcagggcct ctcagagtcc tgccgtggtg tgcactgtgt gtgtgtttaa ttttctacat 21780
ttggatgtga tcctaatcca ataaatgctt aggagacttc tatagaatag attaattttt 21840
actagaaaaa aatataattg gctgatgtta aggctactgc cctgacaaat ctgccttggc 21900
catatatctg agaaggtaaa agacccgcta cgcttgcaca taaatatgcc atcttcccca 21960
caggccctgg agaagcaccc cggggaggtt tcccttggtg atttattctt cattaataag 22020
ctctatgcta tattaggatc agatttatga ctctgccttt ctaatatttc tgacatttca 22080
tctgaaaaga attacaaatg aaatcttgaa actttgccac ttctccctgc tagtgctctg 22140
gcactctgtg tccaagggga gatggtgggc tggggagacc ccaagaagca gggacagagg 22200
catgtttctc agggaaagga gcgatcagct tgactttggg agagctttat tcagtttgca 22260
agcagcttgg gaggtgagcg gttcaggcga gaaggctgca gaccagacca caagcccagc 22320
agcagcagtg atgcctgtaa catgtatgag atggtggcag gcacattcat tcattcaaca 22380
gctatttgtt aagcacttta ctgtgtgcca aacactgtgc tgcttggtgc ttgggataca 22440
ggaaagaata aaagtgaagc agtgatgaag atcttggcct tcatgcatat ggtgttctag 22500
cagaggtagg ggagtgtcaa gcactgatga atgaaaatcc tgaataggta aaatatatca 22560
tatgttcaca cgtggtaagg gctatggcaa aacagaaaca aaccctccac acagggaaaa 22620
gggaaccaag agtgccgggt ggtcaagttg caatgttaat taagaaagca cttcaactca 22680
tcaactcaag gaaccgtatt atccccattt tacagatgag gacggaggct cagcgtggat 22740
taggagctac acaaagacac aaagggaact ttgcacaact tgaaagtttg caaagtgccg 22800
tctgagccat tagtctccct tctccccact gactgcccta ccacaatcac atggggacca 22860
taaaaatact gttgcctggg tcccgctccc ctgaggcttt gattcaattg gtttggatgc 22920
agcctgggac ttggcatgtg actcttgtgg tgccaggact aagaatcttg tgtgaactcc 22980
cagcacagcc tctcaggcct ctattttctc acctgcaaga tgtgggtaaa aatattcacc 23040
ctgtactgca agaggatgtc aggagtgaag gcagaaacac agcaccagcc ctccaggacc 23100
cccaacccct cccccacccc aatccttcac ccctgttgta ccttctgacc tcaaagtggc 23160
tctgattatt tcactcccac aggccactgg ctcagaggta tagagctcac ctgtggcaga 23220
tggagatgcg gatctgaggc ttctgatgct gccacaccca gcggcgcccc ccaaattccg 23280
ggcccctgga tgacatctgg tctgttcctg cagcatcaga gcacaataga gccagccacc 23340
agtcccagcc ctgcctgcat cccatccatt cctgggtgcc taaccccgag gatcccctgg 23400
cagtatgatg cggacctgtc ttggatccca gggatatgct ggccacgggg aggagccgga 23460
aaccaacctt tgtgtcactg tgtagtgaca agtgcctttg gaggtcacaa tagccagtgg 23520
tgatttctac cactgccccc agcagccaag gtggcagagg agccctgtca gtcaccccca 23580
ttctgttcat ggtctcacgg tgggctccac atggggggtg gcagccctct cccccacccc 23640
acccgacccc tttcgacaga tagggtaata caaatacaaa taacaccaaa agattgagtt 23700
gctgggcaga aagggaccaa aggccagtgt gtgtgtgagg ggtgggggca gggcaggaga 23760
ggagcagcaa aaggctgtga ccgcctggct gagcactgga tactcactga agggcaggga 23820
ggcttcctgg agaaggagac ctggcagggg ctgagggagt gatgccaggc atgggggttt 23880
ggagggaccc caggcatggc atgcctccat tcctccctgt gctatccact ctatataagg 23940
ggtgctgtgc agggagacag cttgcatcca agcagggagg cagggaggat gagaggcaga 24000
gaggagccca gctgggttga tggaaagtct gggaaatgca ggaaatccag gagggggaga 24060
atgattccaa gctgtggcct gtgatgggcc ttgaaaccag gtgtaggcac ttggatctga 24120
tcgctgggga gccagagctg cttcctgagc agcagaaggg caggatgcga atcagactag 24180
gggcagtggg aggaactgag aggcctcagg tcaccggaga aaatgcacag ggccgggagg 24240
cagagatgct cctgttttct tgctctgggg ctcaggacag tcagtcaccc tgagcttcag 24300
actcagctca ctcattttgc agagatcctg acggcgatgc ttcaggatga tctgggaaga 24360
gtcaatgagg taaaatatgt gaaatatgcc ttgaaaacta caaaccacag cacatgttct 24420
gtttttgctt ctgcttgatg gactcagtga gatggtgggg acaagaatta gagaagccca 24480
tgaggaggcc aaggggcacc aaatagaccc accaaggacc agtggggact tagagaaagg 24540
atgagtcaga gagaaatgac aggagcagaa ggcaggcctt gtatggagga tgaaggtgaa 24600
gatcatacac cattaaactt gagaaagagg cgggaggagc tgccatttct tgagtctact 24660
ggatgccagc agtagtgctg ggcaagggct taacaggtgg ggaaatcgag gcacagagag 24720
gttaagtagc ttgcctaaga tcacccagtt agtaagtagc agagcctggc agcttaactc 24780
caaagtcttt gtactaaagc cagattttcc aaatttgccc aactgtaaga atcacctggg 24840
cctgtaatcc tagcattttg ggaggctgag gtgggtggat cacctgaggt caggagtttg 24900
agaccagcct ggccaacatg gtgaaaccct gtctctacta aaaatacaaa caattagccg 24960
ggcgtggtgg caggtgcctg taatcctgta atcccagcta cttgggaggc tgaggcagga 25020
gaattgcttg aaccccaaag gtggaggttg cagtgagctg agattgcgcc atcgcactcc 25080
agcctgggca acaagagaga aacgccatct caaaaaaata aaagcccggg agtttattac 25140
agatgcatat tcccaggcac ctcctacgga ggttttgagt tagtgagtcc aaggccttct 25200
gcctcttccc aatgtattca ttatgcacca tcattactct tgttcagata caatgtgagt 25260
gatagcttgt ctctggcagc acagcagcca cacccaacca atccagacct cagtcatgag 25320
ggtgccaatc acagctaaca tttttttttt tgagacggag tcttgctctg tcgcccaggc 25380
tggagtgcag tggcacgatc ttggttcact gcagcctctg cctcctgggt tcaagcgatt 25440
ctcctgcctc agcctcctaa gtagctggga ttacaggcac ctgccaccat gcccggctaa 25500
ttgtattttt agtagagatg ggggtttcac tatgttggcc aggctggcct tgaactcctg 25560
accttgtgag ccacctgcct cagcttccca aagtgctggg attacaggtg tgagccaccg 25620
cacctggccc acatagctaa catttaatca gcacatacag ggccatgctc atcatttttt 25680
gcgcacaatg ccatttaacc ttcacaacag cctgtgagaa ggtgtgttag cctcatttta 25740
cagaggaaga aactaaggcc cagagaagtt atgcaacttg cccaaggaca cacagcttga 25800
aggagctgag gtttaacccg tttctacggg gtctgaatcc tccttaccac ccctatctcc 25860
cctgactccc aggttgtgtt tggtgtactt gggtagtgtc cagctgacaa atgagatgtt 25920
ttagcttcag acagtctatg ccatgtagaa atgcacccag gactgtgcat attagggagg 25980
tttgcaaatg tgtccacatt agataatttt tctcaaaatg cccctcactg aacttctgtc 26040
atgcagtatg tactgagcac ctgcctcttg ctaggtcccg ggagagagga tgaagagggc 26100
agagccccca ccctcagggg acctgaaaaa tgggagtcct ttgtgctcat ggaagcaggg 26160
ccttggggcc tcagcactat ggacatttgg ggccgggtaa ttctttgttg tggggggctg 26220
tcctgtagat tgcaggatgc tccgccgcat cccttgcccc tgccctctag aagccagtag 26280
caccctccag ttgtgaaagc cgtaaaatgt ctccagacat tgctcccagt tgagagccac 26340
ttcattaaag aaaaaaaaat aaaaaagatt tctgtatcct ctatacatgg actgaatttc 26400
actgacattt ctctaaaata actgaatcta tttctatact catttttttt taccagctgc 26460
cattttattt atttttcttt tctttctttt tttaagaaac agggtctcac tctgtcaccc 26520
aggctggagt gcagtggcgc aattatggct cactgcagcc tcgacctcct gggctcaagc 26580
aatcctccca ccttagcgtc cccagtagct ggcacgccac tgtgcccggc tatttatttt 26640
attttatttt attttttttg tggagacagg gtctcccaat gttgttcagg ctggtctcaa 26700
actcctgggt ttaagcaatt cttctgcctt catttcccag agtgttagga ttgcaggcgt 26760
gagccactgc acccggccat tttatttctt aacaaagcac aaatcaaatg tacaatgcag 26820
ctagattttc ctttataaat aatgtctaat ggatttgttt ctgtcagctg cctagaacat 26880
tctggtatcc cagacagaag tgcaacacca ggtggaggtg ttgcagctga gaagttctga 26940
ccagcatacc agcaccccct taaaagctgt ctatacttgc ggttctcagc taggctgcca 27000
cagaatcgcc caagtcttaa aaaaacaggt ctctgtccta ctgcttgaga tgctggttct 27060
ctaggtctga ggttctggca cctgtgtgtt tttaaaactt tacaaataat ttaaaagcgc 27120
cttcaagtcc acaacaacag aatatttccc tccactcctc attgtcctgg agttctctaa 27180
cagtgcagtt ctacaactgg acacacgatg tcgcttttca gccacagttc tcactaagcg 27240
gccccacagg gcggcaggtg ccttctgcag agagagagag agggccttgg ctgacaggcc 27300
aagaccgggc atcctggctc ctcctctgta cagacttttc acagatgtga actctcccta 27360
ctccctgtct tctgccccca aatgaagcct ctcagctggc aagagctgag aactaccaag 27420
cgagccattg ctaatttcta ttgtgtttgg aaccacaaaa ggcagaatta ttaaggctgt 27480
aaaggacctc agagcatctg gtgcagtgag tttccaactt gtgaaaatct gatgtgatct 27540
cggacaagtc acttaacccc ctgcctcaat ttcttcatct gtaaaatagg ataggaatat 27600
atcttgcccg tctgattgtt atgaagacaa agagaacaaa tgcacataaa gcccgtgacc 27660
atgtgctttg tgaatggaag ctttaattta ttcattcatt tattttatta tttattttta 27720
tttatttatt tatttttgag acatagtctc gctctgttgc ccaggctgga gtgcaatggt 27780
gcgacctcgg ctcactgcaa cctccatctc ccggattcaa acaattctcc tgcctcagcc 27840
ttctgagtag ctgggattac aggtgcctgt caccatggcc agctagtttt tgtattttta 27900
gtagagactg ggtttcacca tgttggccag ggtggtctca aactcctgac ctcgtgatct 27960
gcccgctttg gcctcccaaa ggaagctttt atttttatta tagttttaca taaggataaa 28020
ttcagcctta gtgaagggaa gtgacttgcc caagatcata cagtgagact gctggatctg 28080
gggctctact tcagaatttt tttttgagat ggagtttcac tctgttgccc aggctggagt 28140
gcagtggcac aatctcggct cactgcaacc tccacctccc gggttcaagc gattctcctg 28200
cctcagcatc cctgatagct gggactacag gctcccacca ccacgcccag ctaattttta 28260
tattttgagt agagacaggg tttcaccatg ttggccaggc tggtctcaac ctgcctggcc 28320
tcccagagtg ctgagattac aggtgtgagc caccacgcca gtctactgcc attgtccatg 28380
atttttccta cagggaaaat cacaatccca gaagataaga caaagaacag taaaaggtgg 28440
cttttgaggc agtgagttct acctgaaggt gggaacagcc cagagtgtct ggggacagag 28500
tggtaaattc taatcaagcc ttcccatggc tttgtggatg aggatgagtt tctaccctga 28560
agctcggcct gttcagccat aaaatcagga taatggtggc tgtgcctcct tagagtagaa 28620
tgagaatcag agcagaacaa gggaaagctg cagtgacttg tcaggtgtca accttcagca 28680
tgatgggaga gccatgggac ccttcccctt tccctaagag agccagccct cacagcaggc 28740
ctgggatcca atgcccagca cccagctggg agcccaggga ccttggccaa aatctggttc 28800
tgcttcaact tggtgcccgg cctttgtcaa gtcacttcct catttgcaaa ctgggagagt 28860
ttggatgaaa ctattgaatg aaattatttt gggggtttct ttctggctct cacagtcctc 28920
gcatgctcac catgttccct tcaatttcat tagcacagcc caacaaaggg ttaagcagtg 28980
gcagttcctc tcgttctttg gttaggacag gaaggtcagg ggtgaggcca atacaagagg 29040
tagccgccac agctgatgct ggaaatgaca atagttcttt cctagactca tatttgtccc 29100
ctctccctga agctttgcct gcagtgccct tgtaaagaag ttggcaagaa gcaggagtga 29160
ggctcagccc ctctctgaaa tggatacgcc ggttgctccc cctcatggct ggtctcattt 29220
gccttcttca tttttagaca cattccaaac ttttcagcaa attatagtgt ttgccaactg 29280
gccgtctggg gcccaggaga gatgctattt atagcgatgc tgggatgctg ccatcccaga 29340
gcagcctggt aagaaacgga gccagagtgc ctgggagtgg cgtcctgcac cctggggaga 29400
ggccagggcc ctggagcagg gtggcaaagc tggtggcccg tggcaaggac cactggcaca 29460
tcccctgcct gcctgggccc tggggtctgt gcccataccc cacacggggg gctgcttccg 29520
tgctccttgg agagacgatg gtgctgtggg gccactgagc acagtaaagg ctaagaccca 29580
ccataggtca gcccttgctc atgctgactg ttgccccatt tcccttcatt ctctcactcg 29640
ttcattcctc agaatctgca tcctggtttt gtcactacct ggagttgtaa agataccacc 29700
aagctcacct tgtggtgtga gccttgattt tccccatctg tgtaaagggt ggatctgagc 29760
tccaaagttc cttctagtca tatgcagagt gcataataaa tgtgtttgta ttcccactgt 29820
gctcagtggg cactggggca tgcagaagag aaattggaat aaatgtgacc cttgtcctcc 29880
agaggctcag taccagactg gaaccaggac ccagatgagg ggcctaccca gagagggcag 29940
cgtgctctcc tatttgtgtt aggcgttacc atttacaaag ggctttacgg ctttgaaggt 30000
ccccacaacg ccctgaggag gtgaagtgtg gcaatgcccg ttcacttggg aaaatactgc 30060
atctcagaga gaccaaggga ctcgcttaag gtcacacaga tacagtaagt aagtagggaa 30120
agagctgggg cctctgggct ccttttccgg ggctctggcc tcggttttaa cccgttctcc 30180
tagacctctc agtctctggg cctcccctct gtgttcccca cccccacttt ccatcaagag 30240
ctcagttctc ttaagttcta tattctctct tccccacccc tagaaatctc tgcccgcttc 30300
cagaaaaggc tttgattccg tcatttttgg catttcccac ccaggaatca aggctgcctc 30360
ctctgtgagg aaggcgtgtg gaaggcgagc agctgaggac acctcttttt aagagaagct 30420
tctccatgtt cttagccagg ttatataact tcttttgctt ctttcttttc cacattctac 30480
atatttttca caatgaggat gtgtcacttc tatagttaaa aatggaaatc ttcatttaaa 30540
agaacaatca gacacaaggc aaggtatgct taccttccca atgaccaagg agcagggaga 30600
cattggtcgt ggaggccata ggtgaccagc cttgagggaa gggaaggaag gggaggtgca 30660
gggaggcgca gcagaggcac ccgagtgtgc tctacgaatg taagtctgcc agctgctcct 30720
ctgtgcctag caccctggaa agcgcccgca cacagtgggc cctcagcaaa ctcccactga 30780
gcaaagggcc ctgtgagtaa ggacctagaa gcagggtgtg cttgaggcat ggggtgggag 30840
ggcatccagc ttggctggcg gtgggagccg atgcaaaggt gagctgagac cagacggtag 30900
aagaccttca gtgctgggcc gaggagggtt ccttcatccc atagagaaga gctgccatcc 30960
aagacagaag gctggggagt gacatgttga aatcagtatc ttaggaaaat aaagccactg 31020
gctgggccac tggctcccct ttgtagaggg gtctacgaag ctcccgaggt ttcagatagt 31080
ccctgagagc ccttcccctc gcgatgggct ccgtttgagg catgtccagt gtgaaaggac 31140
cacaggccac ctgagggaca ggaccaagca gagagctagt gacagaatgc ccagggctcc 31200
aagacagggc tggagaggtg ggagtggtcc ccaccttgag agccaaaggg gctgagggct 31260
gagggttgaa ggccgagagc caggaaggtc caggggagca aaagggggag cagaggggga 31320
gctggaggtg ttggggtggt gggtagagag ccaagatagg gaggggacaa gagaggggag 31380
aatctcaagg aaaagcagga gcatgagact gagagaaggc ccctggaaca ctggcttgaa 31440
tgtggctgac attggcactg ccagtactca tcccaaacca gggagctcag ccagcgccct 31500
ttctgcatgt ccgctctttc atcagacagc taggaacaag ttatacgaga tgatgtccaa 31560
gtccaataaa tgtctaagtc ctttccactg gatctggccc cagcctcctc tctgaaccat 31620
ctcctatcac tccaccctat ccctgctggc tccttctgct ccagccatac aggcttgctc 31680
ctgggctcag cttgtgcctg cctggggagc ctcgcgcttg ctgtttcctc cttgagaaat 31740
tcctgccctg gataactcct tcccccactt ccttcagatc tctgctccct tatcaggggg 31800
cttcccctgg ctgccctatc tataacagcc ctgaccactc ttttcctctt ctcctgcttt 31860
ctttgtcttc ctgttcttat cactaagaca tcacacatgt cttagttggc ttgtttatat 31920
tctgtctcca cccatcttag tcagctactg ctgaaatgat gctgtgtaac aaaccacccc 31980
gatactcaga ggctccaaac aagtgctgat ctttctcatt tgtgagtctg tgtgtcagcc 32040
cgagcagggc agctccaggc tatggcttgg gtttgggtgg actccatgtc tctctccatt 32100
ctccttggac cagcagctcc ctggggcaca tgcttgtctt ggagaatggc aggaacttaa 32160
aagccaagcc aaacctcaca gcacggttaa gggtgatcat gacacaccgc taacattcta 32220
gtggccaaag aagtctcatg gccaagtgta atagcagtgg attgggcaag tttactctct 32280
tacacctgtt gtgaagggga gtgggagggg atgggggggt gaccgttttc acaacaacag 32340
tgcaaactat cccatctccc aatagaacat aaacacctta aggttggaac tgggtctgta 32400
tccccagcac ttaaaatagt ttttggcata cagtaagggt gcaatagaca tttgttaaat 32460
aaatatacag actaaccaat tagcataata cagatgacaa aggtgtctct cttctgccag 32520
cttctgtgcc tgaaatgttg atgatctcct ccctatcttc ctaacatctc tcctgtcccc 32580
acctgtccat gcccagggtt tggctgctgt gacagaagtg tgagagcctc ctgtctccct 32640
tgggatccca gtaagagctt ccatgcctct ctccctgctc acctgggctc ccatccctgg 32700
ggaccttctg gaaacagctt ccagggctcc cagagcttac ttagccagat tctacatctg 32760
gctccagctg ttatcctaag cttggccttg ttttctgatc tgaccacagc ttcatcactc 32820
ctacctgact gtgggattct agcccccagt ggggtggggg accaagggtg acaattacct 32880
gggaccttga ctattgaaag gctaatcagg tttgattggg aaaaaagagt tgctaaaaag 32940
gattgtattg gataattggt gagatttgaa catggatttt atattagtat tgtgttatta 33000
cattttctga tattagtcat tctttttttt tttttttttt gacggagttt gctctgtcac 33060
caggctggag tgcaatagtg tgatctcggc tcactgcaac ctctgccacc cgggttcaag 33120
cgattcccct gcttcggcct cctgagtagc tgggactaca ggcgggtgcc accacaccca 33180
gcttgttttt gtacttttag tagagacagg gtttcaacat gttggccagg atggtctcga 33240
tctcttgacc tcgtgatctg cccgcctcag cctcccaaag tgctgggatt acaggcatga 33300
gtcaccgctc ccggccgtta gtcattctta taggtatgta agaaaatgtc cttgaggtat 33360
ttagcaggga agttcatgat ctctgcagct tactcacaaa tggttaaaca gaatgagact 33420
aagaacagac tagtaagaaa agactgaaga atataattat taggccaggt gcagtgactc 33480
acacctgtaa atcccaacat tttgggaagc tgagatagga ggatcacttg aggccaggag 33540
tttgagacca gcctgggcaa catagtgaga ccccatctct acaaaaaaaa aaaaaagtaa 33600
aaattacgtg ggcacggtgg tatacgcctg tacttccagc ttcttgggag gctgaagtgg 33660
gatgatcaga ggacctcagg agtttgaggc tgcagtgagc tatgattgca gcactgcact 33720
ccagccaggg agacagactg aggcccggtc tctaaaaaaa aaggaatcta tgtgagtcta 33780
tatattcgtg caacttttct ataggattga aactctttaa aataagctta ataataaaaa 33840
caaaagctgg tgggtgagac ctttcatgta ctctccagga gagttaagcc ccccaacatt 33900
cctgtcccct tgtttactct caagcacccc ctcccccacc caaggaccag gtctttgttt 33960
actgagcatc tcagcgatga gctctcaccc cctgatttca tcaattataa atgtgctcgc 34020
tactcaccac acggcaattt gtgacggact gtggtttgtg gtgagagtag caccatccaa 34080
gttcaccgca gccgcgagta gagatgaggg ttggggccag acacagggct gtgggggcgg 34140
caagggcacg caggcagccc tgccaccttc ctgtttgtca gccaagtgag gcttccgagg 34200
gcagcgggcg agcgggtcac tactcagggc cagctactgc ggccaggcca ggctagtcag 34260
gtctgtgcag ccagaactag aggctccgcc aggatgtgag gtctcccagc tcctgggaac 34320
tgaagcaaac actctgacta ccccttcttg aagtgcctta cggtgtatac atttgtttaa 34380
tctgcacaac aaacctagga gcagttattg ttactgtcct cattttgcag atgaggaaac 34440
tgaggcaaag agaaattaag taactccttc aaagtctggt aagtgacaga accagatttt 34500
accctcttac tcattgtcca tattgccgag taacttacat taatagatac tatgcatgtt 34560
tattttattt tttatttttt tttagagatg gggtctcatt cttttttttt tttttttttg 34620
agacggagtc tcgctctgtc gcccaggctg gagtccagtg gggcgtgatc ttggctcact 34680
gcaagctccg cctccctggt tcactccatt ctcctgcctc agcctcccga gtagctggga 34740
ctacaggcac ctgccaccac gcccggctaa ttttttgtat atttagtaga gtcggggttt 34800
caccgcgtta ccagtatggt ctcgatctcc cgaactcgtg atccgcccac ctcggcctcc 34860
caaagtgctg ggattacagg cgtgagccac cgcgcccggt cctcattctt tcactcagac 34920
tggagtgccg tcgtgcaatc tcagctcact gcagcctcaa ctcggggctc aagcaatcct 34980
gccacctcag cctcccgagt agctgggacc acaggcagtg ccaccacacc caactaattt 35040
ttgcattttt tgtagagacc ggcttttgcc atgttgccca ggctggtctc gaactcctga 35100
gctcagacaa tccacttgcc tcggcctcca aaagtgctgg gattatagca ccacgcccag 35160
ccgatactat gcatgtttaa ctgacaccta atgattaggt agcaacaatt tcttggctgc 35220
cttattcacc ttcatgaccc ccaacattga aaatgtcttt tcccatgtga aagcatttgt 35280
gggctcctac tgccaatcta taaagtagaa atccttctgt ggtacaaagc cctctttctt 35340
atctaatttc ccatttcccg ttcccccttc ccccttctgc taagagctct tccctaccca 35400
cattgtaagt agggccgggg agctaactcc accttgcgta ctccaaatca accacatgac 35460
ccaggcctga ccaatcaggg ctccaaatcc tctagctata gtgattggtt tagggaggga 35520
catgtgaccc gcctgagcca atgaggatct gttctgggac tcctgtttga actcttggga 35580
aaataaactc cttatgttgg gtggctgagg ggatgaatgt gagctgggag ctgcaagcag 35640
gcatcatgct attcccttca gctttcaagt agtgttttca ctgctgttaa caattccaaa 35700
tcttaagtgt tccttgcatc ttcctctcgc caaaaatatt acagtactgg agggcttaca 35760
tggtgtctca aatgtctgga gtttaaatct gccttgcagc ttccatggcc acagtgagtg 35820
tctgagtcct ggccgctttc tgctgatccc ggggagagtt tagcctgcct gcccttcctc 35880
cctggttcgt tctcatgtac tcagatccct gcctcactct tctctcacac agacaacaaa 35940
agagagtcag attgtggtct taagtgcaca ggactctgaa gttaggacca gaagaccttg 36000
gttagagtct aaaccttgtc agttaccaaa tatcattagg cacattagtt aatctctctg 36060
aggcttattt ccccatcagt aaagtgggta ctgctgaaga tgtagtcttt agaagtgttc 36120
tctagattgt aagccccgtg cttcctggtt attgactaaa cagggataaa aatgagtttc 36180
tcctcacctg ggtgtcattg agtttctggt cattgtgaga tgggtgatga agccaagggc 36240
atcaaacatc ccaacgaggc tgttttctgc atctccaggg ctatcccagc gttaatcaca 36300
tatggacctt taatcagagt ttgcagaata agtgagaatt gtgttagagt atgaatgtag 36360
atattatctt gttttagtca ttgttactct aggggaccat tcttactaca ggaatattgc 36420
acaaaaccca gaaatttatt gactttctcc taaccaaggc ctaaagagct ggtgttagac 36480
ataggccaag gccagccaga ggcccaaagc ctgtttccca gggtaggact gccctggcct 36540
ccccctcttt ctccccaggc tccaccccag agagctgaag accaggctgg gtacggcact 36600
gctgagaaac tgaggaaaag gccactggcc tcctctctca ctgcaggctg cccacccggg 36660
agggggaaag cttgtcacta aatcaggttc agttttggtc actgtcttgg actggatatt 36720
ctagcatcag aactgagatg tttcttgtga cttaaagtaa cttcaggact ctattctacc 36780
taggattggg cagaaaagtt atgggcctgc gggagttcca attcagaaac aggggagatt 36840
acttgcacta aagaaagtct aaaggaaggt aggagacaaa aataaagttg tgtattgatg 36900
atcctaggag ttatgcttgt ttgacatacc agttatacct gctgtcacgg tagttatgca 36960
ttaggggacc caggtgtctg aagttatatc cagaagactt ctgagggtgc accggggggt 37020
cccttggcta aaagtgtgat ttaaacccta agagcctgcc cagactatca gtcccagttt 37080
ctacgtccac tgtccctgaa tctcgctgct tcttccttag gctgctggga gtctgaaccc 37140
tcccccgcca acacccctcc cccatgcctc agtcgtggga agggggggcc cttgagcagt 37200
agggccaagc cctgttcagc ctgggaccaa gttcccatca acaaggtggt ctgggcagtg 37260
gccagccaga aagcagtaat tactgtcgag gtgcagggac cccaggtagg gcccccacct 37320
cccacctctg tgtgggcagt gaatgggcct gcccctgggt aaggctgtgt cagcaggcgc 37380
ctgcccaccc cttgctgggt tcccaggccc ctagagccct ctcgtaatag gagccatttg 37440
cgctgtaacc agtgggtgac cagattttta atcttggaga ccccttggat cccaggcggg 37500
aagtgggatt tgtcaaatgg ggagaggcgg ggctgtctgg gaatgccaga cggggttgtg 37560
ctggggaaat atgtctcctt tccatacagc ccccttccca tacctccagc ctccctctac 37620
cccgcaagtc agctctgtag ctcctaggag gtatctccaa catgcttagc tgttgaaagt 37680
aaatgaatgc cggaagttga aatctgaatg gcttgttttg cactaactca ggtgcatgcc 37740
aaataggggt gtctctttgc ttgatcctaa tccttcctcc tctgaaatcc tttctgacct 37800
gctgcctatc agcaattgcc ctgcaaagac cctctagctg gccgcgggag aagctgtgtt 37860
cttggctatc aggagtgaga acactggacc caagcttgcc tattctctgc cacaactcac 37920
tgtgtgatct tggacaagtc acttcctttc tttcagcctc agtttcagga gacttggcac 37980
ttgctgagtg ccaatgtgtg ccatgttctt gacaagtatg gtcccacaca accttgccaa 38040
tgaccctgtg acctgtgtct tcttgttccc agtttacagg agagaagatg cagcctggga 38100
gagatgcagc aggtgtctga ggccacacag caagtcaccc agggccagga tctgaagctg 38160
ggtctctcca gctccactgc ctgggcactt tctcctccac agcgaccttc aggtcatcat 38220
gaggagcctt tcggactaaa gctagagagc tgggattcca acagttcagc aacccatgac 38280
ttctccatgg cagctgctgc ctgaccacct agtgcctttc cactaagatt gttccttccc 38340
tctcctgaag attatcctcc tgcccctctc tcccaaacat ctgcggtgtg cacctgctgc 38400
ccaaagttga actttttttt gttagagaca gggcctcact gtgtcatcca ggctggagtg 38460
cagtggcgtg atcatagctc actgcagcct ccaactcctg ggctcaagtg accctctcac 38520
ctcagcttcc tgagtagctg ggactacagg catgcaccac cacgcctggc taatttttaa 38580
attttttgca gagacagggt ctagctatgt tgcccaccct ggtcttgaac tcctgggctc 38640
aagcaatctg tctgccttgg tctcccagct cgctgggatt acaggcatga atcaccatgc 38700
ccggtcctcc aaagttgaac ttttgagact cagtttcctt cttggtaaat tcaggctcct 38760
aggtgctacc tcctcagaga catcctcctt gaccacatca catgacctct cgcagaggcc 38820
agattcttta atgtattcat ttgtctatta tcatgtttcc ccagtagaag ttgcacaaac 38880
ggcaggggct tcatctgttt tgtttgccgt tatgtcctta gcacctaaaa ttgtgcctgg 38940
cacatatagt actcagtatg tatttgctgg gtcaatgagt gagtgaattt atactaataa 39000
cagcagctac catttctaga gtgtttacca tatattgggc actgtgtcag tcttcccaac 39060
aacccacaga cgaagatcaa ttattacacc cattgtacag atcaggaaac tgagtcaggt 39120
taagaaactt gccctaaatc ctacagtctc acttagaact tctgactgca gtgctcatca 39180
gaatgcattg tcaacccaaa ggtcatttcc agctcaggtg gcttctatca aaagagctca 39240
tcctggcctt tccaagagcc agacctccga catcggtgga gccctgtgca tagctggcct 39300
ctcctgggcg tcttgtccca agtacagaga cctggatcct ttcccactca tgtgcaacag 39360
cccaaaatta aaaacaaaag ccatattaaa aaacaaaacc aactttctgc cttaaaatat 39420
tgtgagccag ggggcaatta gcaattatgc tgtattttat tatgagaaga tagaattcta 39480
attggactga tttgaattcc acacacctcc acagattgtt ttgggaatta aggtatcagt 39540
tgtatcggta attatggttt accattcaat taccccccca cagaaaactg ttaaattgtc 39600
tgtgacgggg cttaaattta gctcagacct atgtcctatg aagactgcgc gagtcaatac 39660
aagccatccg gaaaccaccg ggtgccctgt gccaggcggt aattaggggt tgaggtttcc 39720
aaagttttac ctgagacagc agggacaagt gcctgggctg ggcgtgctca cgtggggggg 39780
cttggatgct cccccagcac agtgctcttg gctcctgccc tgcgttgctg gtgcaatagc 39840
tgatcatctg gaagacaatg tggtttcagc cgcaagtgac attttggcga ggtgcaccag 39900
catagaagcc ctgagacagc gagggaccat gtaaaactca cggacattgt aattggacac 39960
atctgggatt gaattcccac tcttccactt agggagtgaa cttgagaggg ccacttaata 40020
tctttgagcc tctgtttccc cacctgtcaa atgggtacac ctcctgcctc acacacgggt 40080
tattgtgaag atggatggag aataataatg gtgcctggcc caaattgcat ttttttctct 40140
ttttttagta ctcactacat gccagatact ttacaaacat ctctaaacct cctaagccct 40200
atagggcagg cactgtggtt attctctgtt ttatggatgg gaaaactgag tcccagagag 40260
gttaaataac atgccgaaag tcccatgact atgaaatggg gcagctggga tccaaaccca 40320
ggtgacctag ggccaaaccc taagcatgaa gctgccctgc tgggtgcctt ctgtacctgt 40380
ccctctaagt ggaagtgcct agaaatgacc cagccaaaag cagcagactg tattttatca 40440
tttcaacaat tctctctgtc cataaaaagt tctatgcagc ttggccagtt cctttttcct 40500
gaatacaaaa ctgagaatgg taacagggaa ctgacttttt aatgtcatgg tgcaaaggag 40560
ctagctctgc catgacctcc ttgaagtgac ctggtagagt gagggtaaga caagccacac 40620
tccctaggct atggaggcca ctctccatgg agatagggga atagggaatc ctgcaaaata 40680
cagtctctgg ggatgggaag gatcagggaa ggggccaggt gcagtagctc atgcctgtaa 40740
tctcagcatt tcgggaggct gaggtgggag gatcgcttga gcccaggagt ttgagaccag 40800
cagtttgaga ccagcctgag caacatatca agaccctgtc tttacaaaaa attaacaaaa 40860
aggccaggcg tgatggctca cacctgtaac cccagtactt tgagaggccg aggtgggcgg 40920
atcacctgag gtcgggagtt tgagaccagc ctggccaacg tggtgaaact ccgtctctac 40980
taaaaataca aaaattagcc aggtgtggtg gctgtaatac cagctactcg ggaggctgag 41040
gcaggagaat cacttgaatc caggaggcag aggttgcagt gagcccagat catgccactg 41100
cactccagcc tgggagacag agtgagactc cgtctcaaaa aaaaaaaaaa acaattaaca 41160
aattagatgg gtgtggtggt gcacagctgt agtcccagct actcaggagg ctgaggtgaa 41220
gggatcactt gatccaggtg ttcgaggcta tagtaagctg tgatcacccc actgcactcc 41280
agcctgggtg acagagtgag atcttgtctc aaaaaaaaaa aatttttttt tttaaaggat 41340
cagggtaggt acatggactc ctcctattcc ttctggcacc atcagtggtt gaacacacaa 41400
actttggttc aaatcctgcc tcttccaatt tgctagctgt gtgaccttga aaaagttact 41460
taacccctct tatccttagt ttcctcatct tcagaataaa aatagtgctt gcctgatggg 41520
ggagatgatg gatgtaaagg gcctcataga gggcttggca tttaccaagt gtttgagaaa 41580
tattggctgt ttttattcaa ggcctgctca ttgctagcat gagctacctg caacagccac 41640
ctctaggcct cttttgctcc ctatagtcca gccacaccaa gaaaccatga tggcctgaat 41700
cctctctgac tttgtcaggc ctccgtgcct ttatacatgc tgttccttct gcctagaagg 41760
cctttctctt ttttcaacct ccttgagtcc attttagtcc ctgccagatg gtcacttctc 41820
tgtgatgctg ccctaactcc ttggcagcca tgctgctccc ttgaccattc actagcccct 41880
ttaattcagc cccagccaca gtgaggatta aatgggatga tgtctgagaa agcccatggg 41940
caagaacctg gcatggagtg gtgccccata aatgatgaat ggagctgggg ttgctttctc 42000
agccagctgc ctggcagggg cctggcttcc tcatcttcac acgtcagtgc ctagagtaac 42060
acctggcaca cagtgaacac aatctcagta actgttagac atttgtcttt tggggagctg 42120
ctcagcctcc cacattgggg tgtgggtttt ttgttttgtt ttgttttgtt ttttgagacg 42180
gagtttcatt cttgttgccc aggctggagt gcaatggtgc gatcttggct cactgcagcc 42240
tccgctgcct gggttcaagt gattctccta cctcagcctc ccgagtagta gctgggatta 42300
caggcacgtg ccaccatgcc tggctaatgt tttgtatttt tagtagagac aggatttcac 42360
catgttggtc aggctggtct tgaactcctg acctcaggtg atccacccgc cttggcctcc 42420
caaagtgctg agattatagg cgtgagccac cacgcccggc ctaggggtgt ggggtctttt 42480
accgactagg cctggtgggg gaagtcaggg accccaccac aaaagttgga gaggccacat 42540
cctcccacct ccatctgggg ttagacatgt gacccaggct tggccaatcg gatgttcctc 42600
catcttgccc agaattcaca ctcagcagtg gtcttcacga ggggtggtag tggtggccag 42660
taggagcagg gctgggcagc ctccctaacc cacgttcatg agacttgaag ctggctggtt 42720
tcctgtcccc tggcctccct tgcccattcc catcctggtg ctccctccct atggactcct 42780
tttgctcata aatatccctc caattaattg ctttgctgct tcttagctag tgttgtttct 42840
gtcacttgca accaagagcc tattcaggtg caggaagggt tgaatgaaca atggaaatgg 42900
atcaacacat gtttctgagc atgtgctgac aggcaggccc tgggcggagt accacggtgt 42960
tcaaatgatg tgtaagatgt gggcagtgcc ccggggggtc tatggggaga cagaccatgc 43020
atgacatcat aaacaagacc acattataaa gtggggttta gttggacctt gcagacaccc 43080
agagaagacg actttgagcc ctcattggat ggtgccatcg caaagtacaa aacgaattgg 43140
gttgtaggaa ctggaggcac tgggacaata ttccagatta gggattgagc tgaaggggtt 43200
ggaaatggct gagtagggtg gtggaaaggg atgcctgcta gtaggcaatt gtcttagtct 43260
gttttgtgct gctgtaatgg attaccacag actgggtaat ttataacaaa cagaaattta 43320
tttggctcat ggttctgaag gatgggaaat ccaaaatcaa ggagccggca tctggtgagg 43380
gccttcttgt tgcatcataa catgccagag ggtgtcacat ggtggaaggg caaagagagg 43440
gagaaaggga aagagaggga gcaggaaagg gcaaacccac tcccatgata ataagctcac 43500
tcatatcatg agtcattcat ccatcgtgag ggcagggtcc acctcccaat accatcacaa 43560
tggcaactaa acctcaacat gatttttgga gaggacaaac attcaaacca tggcagcagc 43620
gtttgtggga aacacgcgcc aaagcaagaa aagaccaacc taagagtgag tgacttggct 43680
cctcattcta cattcatttt catccaatgg ggcccaaggg catgtaccca ttacccatct 43740
gggcagttcc cttgaatgtg ggcttctgtt tgcccgtgga ggtgaggaac ttcaaggaag 43800
aaaccatgaa agacctcttg aggctgaggg ctggcaccag caccaagatc tccaggcagc 43860
tggaacagtg atggctcctc cgtcctcgca ggcggggcac ccaacagggt gtgaccgtca 43920
cctgagggga gacagccaga ggcacaggcc tgatcctggg actgaggttg gcggtttggg 43980
tggagaggtg attctgagtg tgacaccctc cagtgataaa gtggggggct tcccagcagc 44040
ccctggggaa acaggctgca tctctggaga caggagatat gtggaggcct gaggggcagt 44100
ggaaagccct gtgtgtctgg gtcaggtctc tccttggcag gtaatggttt gtcccagtag 44160
ttttcagact ccccatcctg ccctgtcctt ctcctgtctg atgctcagac ctgtcactcc 44220
cagctcagcc cccaccttac tgtctacccc tcagagtccc tcccaccaag ggcaccttct 44280
gtcccacatc tccatggtgc agtcatggga ggagaacttg ggagcataag aaactccacc 44340
aagttggggc caggcatggt ggctcacgcc tgtaatccca gcactttggg aggccgaggc 44400
tggtgaatca caaggtcagg agtttgagac cagcctgacc aacataatga aatcccgtct 44460
ctattaaaaa tgcaaaaaat tagccgggca tggtggcagg tgcctgtaat cccagctact 44520
cacgaggctg aggcaggaga atcacttgaa ccgggaggca gaggatgcag tgagccaaga 44580
tcgcgccatt gcactccagc ctgggtggca gtgaaagact tggtctcaaa aaaaaaaaaa 44640
aagaaagaaa gaaaaaaaaa gaaactccac caagggcttt tgggtcctag ggacagcaat 44700
gatgtggctg gtccagccag aacctcattt tgtcagtaaa gttgggaccc atattcccta 44760
agagatttgt cccaagaaca catgggttgc tacagcggag gagaaatcaa gtctgttttc 44820
cctctggtga attgtcccct gaacgtgctt tcttcctaca gtgttgccag gaaagtaaaa 44880
aaaaaaaaaa aaaaaaaaaa aaagtccacg atgtcagctg gggtgatacc aaaacaattg 44940
tgggaggaac aacatccgca aattgaatag tgtgaggagt gtggacagaa gatgttttgt 45000
ctttggcctc atctcccaga cttgatcttt gtaaatacag aagtttccac cagagccgaa 45060
cctggcaatg acttgaggag cagctgcaag gaagacagcc tctcccaggg tatcacctgg 45120
gggcacaccc cagcttcccc tcctgagcct catcgagggg ttagtgctac ctctcgggaa 45180
aacataaaga tgacaagaag ccaaaggtgc caatagttcc catttagtat aaaagctggc 45240
tcagcaaatc atgctatttc agggcctagg gtgggccagt tcccaggcag ccctggcagg 45300
aaggactctg agaggcggac agtaaggtag gggcttggga gtgataggtc taaacatctg 45360
atggaagcaa aagggaggac aagggaggga gtcaaaaaat ccgggaagcc tgtccttcca 45420
gaaagtaccg acaccagggt gaggtggatc ccagcacccg cacctctgaa tggcctttcc 45480
cctgcacccc tatcccgccc ccactcggag ctaccccaag cacctgttcc tctgggcccc 45540
aaggtgacgc cctttgtgtg tgtataggaa aggacggtgt acacatttgg ttagttcctt 45600
cttttacacc ataaattatc agagacaacc cttttggaaa gcaatttggc aataatatca 45660
agtgacataa agatgttcat agcctttggc ccaataatct tcctcctggg aattaatcct 45720
aagaaaataa ttcacaagga agaaagaacc attttgtatc tacaaagaca tttattgggg 45780
tgttatttat gatagggaaa aactggagac accctcccca gccaacatag agggatggtc 45840
ctataaatta tgatacatcc atccaatgaa atgttaccct gccagtaaaa atggtaaatt 45900
gaaaattgtg tagcaacaag aaagagtgtt cagaaaataa aagcaagtga aaaaaggagc 45960
acaggattgt ctatatgctg tgatcaatgg caatgctgac aaatccatgt atgtgtagat 46020
agttgtagtg ttacctgtaa ggcacgataa ggcggatgta actgtgcttc aaatgttctt 46080
ttccatagct gtggagtcct tctagtatga aatcattttt gtaaaatcgc aatttgtcac 46140
caggggtttt gactttcctt attgcctcgt gggaggtggc agaggcagca catctcagac 46200
cgagcctcgg ttcctccccc tacctcccac cgctgcgctt gaagaaagat gctgcagcct 46260
ccccagtccc catgccagcg ctcccacttt tctctgagct ttcggtggca gacagcgcct 46320
tgggcacttt ttcatgctca taattcgaat tacctgttta agtcggtcaa atgaaaaaat 46380
accagctccg cccccacgcg ggctggccgg ggcgcctgga gcgccagggc ggctgcagcg 46440
cgctctccgc ggccgtcggc cctgagctca tttcctgggg cgcgcgcgcc gggctatttc 46500
agcctggcgc tgtgcaaaca ggacaattta ctgcggccaa aagggaccca aattacaatc 46560
gtatcacaga caaatatccg ccacgccagg tctccagggg ccaggagggg cctctctccc 46620
ggcgcggggg gcgggcgcgg ggtcaggcag gtccgcgggg ctcggctcgg cctcgccgtg 46680
ccctgatcgg cgtttgccac cgagctgtgc ctgctctctg caacaggaag gggcccagct 46740
cccccgggcg accgttccta tctgagttct cgttcctatc tgggttttcg tgcagaaaaa 46800
cttcatctct tcccagggat tttcccctga tttagggtcc ccttttattt ggtttctttt 46860
cagcacttgg gatgaaaaca tccctccatc cagccactcc agggctcaaa gtcatatctc 46920
ctcgttcaga aacctcagtg cctgtccacc actcttccgg aaaagtcgga atccttagtg 46980
tgatggtcaa agaccgctct tagtccagtc cacctgtcca gggccagccc ctgtacccag 47040
atgctccaac cacggagagc acatcagact ttcacaattt tatccttgac aattgccttt 47100
tcatcctgcc taaatgttcc cttgtttctt tctgcctagt gagctcttat tccttcttta 47160
agacccagat acacatcacc tcttccatga agccttccca gacttcccct ttccctgctt 47220
gacatctgcc ttccaagcag agtcagcccc ttttccttcc tagcaccgtg cagaggtaga 47280
tggtacttct cacatcctgt aattaattgc ttgcatgcct gtggtacacc gtaccattgc 47340
tcacaaatat ttcctcttcc tctctggaga aggattacac ttccttgatc cattggcctc 47400
actcaggctt cgccacatga ctggtgctgg ccagtgaagt gggacatgtg agttactggg 47460
gagcagaaac tttcaaagcc aagcatggct caccttgttc tctcttccct ctgttacaag 47520
gatggcagtg ccccagccag aaaccacact gccagcctgg cataggagtg gagcagggca 47580
cacataagtg tgagagaaat gaacttcacc tcagtgctcg aggcccctgg gaccatggac 47640
tcatttgtta ctgcagcata aactagctgg tcttgacttc agcttctgcc tctcttccct 47700
ggctttgcct tcacaaaatc aagagtctga tcttactcat attatagagc tggtcttcaa 47760
ccatgttctt tttttttttt tccttttttt ttttttgaga tggagtttcg ctcatgtagt 47820
ccaggatgga gtgcaatggt gcgatctggg ctcactgcaa tctctgcctc cctggttcaa 47880
gcgattctcc tgcctcagcc tcccaaatgg ctgggattac aggtgcttgc caccacgcct 47940
agctaatttt tgtattttta gaagagatag gatttcacca tgttggccag gctatctcaa 48000
actcctgacc tcaggagatc tgcccgcttc ggcctcccaa agtgctggca ttacaggcat 48060
gagccaccat gcccagcccc atgttcttcg aatggccaag aatatcagta ggtaaggttt 48120
gttgagcatg gattgtgtgc caggtgctgt gctatgcctt tggcaagcgt tatcccatta 48180
catcctttaa cagcactctc tggtgggtac tgctcttata caagatttac aaaagagaaa 48240
agagactcag aggaatgtag gggctcacgt gagattgtaa agtagtgaga agtggagctg 48300
gtccttcagc tcaaatccct ctgatgccaa agaactcaaa ctctgcagtt ccatcatgtg 48360
catcctcact ctctcacttc ctgcagagga tctgtggagg ttgtaactat tgttttcacc 48420
tgtctcttcc actgggctct gagctgcccg aagacagatc tttttttttt ttttgagaca 48480
gaattttgct cttgttgccc aggctggagt gcaatggcac gatcttggct caccacagcc 48540
tctgcctctt gggttcaagc gattctcctg cctcagcctc ccgagtaact gggattacag 48600
gcatgcgcca ccatgcctgg ctacttttgt atttttagta gagacggggt ttctctgtgt 48660
tggtcaggct ggtcttgaac tcccgacctt aggtgatctg tctgtctcgg cctcccaaag 48720
tgctgggatt ataggcgtga gccaccacgc ccggccccga agacagatct taatcatctg 48780
atttcccatc cattaattca gtctttcacc aaggatgtat tgagtgccaa ttatatgcca 48840
ggcatcatct gatgtgttgg ggacagagaa ataaaacaga taaaaatccc tgctgttaat 48900
tctagcctat ggtgacaaga agcagatccg tggctgcctg gggtgagtgt ggggtaggaa 48960
atgggagaaa tggggatgac tgcaactttg ggggtagtgg aaaatgttca gtatcgtggt 49020
agtggtggtg gctgtacagg tatatatatc cgcatacatc ccacagttca gaattataca 49080
ctttaaatgg taaatggata cagtttatta tatgtaaatg atccctcaat taagttgatt 49140
tagaggaacc tggccgggcg tggtggctca cacctagtat tctcagcact ttgggaggcc 49200
gaggtgggcg gatcatgagg tcaggagata gagaccatcc tagctaacat ggtgaaaacc 49260
catctctact aaaaacacga aaacaaaaaa ttagccgggt gtggtggtgg gcgcctgtag 49320
tcccagctac tcgggacact gaacctggga ggcggagctg gcagtgagct gagatcgcac 49380
cacttcactc cagcctgggc aacagagcaa gactctgcct tgaaaaaaaa aaaaaaaaaa 49440
gaacccttgc tcttatggag aagcattcta ctggggaaaa cagacaataa acacatagat 49500
aaataaaaca tatgtcagaa aggggtaagt gatgctcaga aaaataggac aagggatcag 49560
aagtgctagg cgttgcaatt taaaataatg gggtctggaa agccaccctg agaagggcca 49620
atggagctat gcccagcctg tggtatcctt cctcaggcag gggactgtac ccccagctta 49680
caagcctttt cgaggcacct tcagggtttg agcccaaaat gcactgcaca ttaagtgtta 49740
ttgatgggct tgtggaaatc tgctctccgt caggctctga tgaaactttt ccaacaggag 49800
actgaactca gtactgggca tggccccctg cacatagtaa gtccacagca aatgtgtgtg 49860
gggtgaatac tgtcttcttt atcctccctt tttctactcc agtccaggta gacggtgtat 49920
ctacccaccc aattttgctg tttctggtac cctcagtggg tcttgcctgc tcttcctcct 49980
tgaaatcatt actcagaggt ctccgtgtca gctccagaca gatgggcctg gtgctccttt 50040
atctcaatac aattcccccg ctcccaggct ccaggggaaa tcctgagcta actttggtct 50100
ttcctaaact ccacacttcc ctttgggcac ctctttcccc attgcggggc tccctttcag 50160
aatttgctgc tttatttatt tatttatttt tgtcagaggt gcattaatga tgctttattt 50220
aaaaacaaaa aacttggcca ggcgcggtgg cttatgcctg taatcccagc actttgggag 50280
gctgaggtgg gcggatcaca aggtcaggag atcaagacca tccagaccaa catggtgaaa 50340
cccagtctgt actaaaaata caaaaaaaaa aaaaaaaaaa aatcagccgg gcgtggcaca 50400
tgcctgtaat tccagctact caggaggctg aggcaggaga atcacttgaa cccgggaggc 50460
agaggttgca gtgagccgag attgggccac tgcactccag cctgggtgac agagcgagac 50520
tccatctcaa acaaaaaaac accacaaata aataaaaaat aaaaacaaaa aaccaaaaac 50580
agtccattcc atgtcgtgtt gaaactgatc agtgtaagtt aaatggtggt ttttaggctg 50640
gacccatgat ttaagctgta cccatccagc tcaaactgaa aaaaaaaaaa atcatttgaa 50700
tgttaaagca atcgttcaga gtcttcaaga agaaaccagg caggaaaatg ccaataatga 50760
tgactggcaa aatcaaaatc taaaacaaat aaactgttta tcaagctgcc gacagaaaaa 50820
gaaatcttgc atggagacta caagtctgga ttttctggga tgaaattgta caggaatctc 50880
agtctacagt ttcctcaatc gctgtggaga tggagctgtc actgaatctg acagagccct 50940
gcactcccca gtccgccgac cctttctgta atccagtctt cactgtagcc tgaggaacta 51000
tttcaacctg ctcctttttt atcttcttct ttggcacaac ctcagtggac ttctctgatt 51060
cagaacaagt tctaattgat cttctctgtt gcttcttttc tactgagcct gtagaaccag 51120
atgttgcttc aagagatgat atattctgca ttggcttttc atttctctgg tttggtttag 51180
aaattataag cctgtcttgc cccctgacac ttatttctgt tttgttacca attccctttg 51240
ttgaataaac aaattaattt cccatcctct gtagcattct gaagagcaaa cacttcttca 51300
attttcacag ctggagacat gttacacttc tgcaaatcca ggctcccttt gtgcattgta 51360
atggaagctg gtaggatttc cttgctgcca cagttttcca ggctatttta acaggaggtg 51420
gctcttcctc gtccgcgctt gtgtgctgcc tcgggctgtg tctccaaatg tcagtacttg 51480
agagtgagga ggccacctcc cctgcattga tctgttctgg ctgagtttaa agcacagatc 51540
ttggtcatca ggttttttta acttcggctt tggagacaac attctttttt tttttttttt 51600
tagatggagt ctcgctctgt cgcccaggct ggaatgcagt ggtgcgatct ccggttcatg 51660
ccattctcct gcctcagcct cccgagtacc tgggattaca ggcgcccacc accatgccc 51719

3

492

DNA

Homo sapiens

3
ggtttcaact agttctggtg catagcagcc cccaaagttt tagcagcgtg gttagtggag 60
agctgtggct tttgcctcca tctaaatttt tctttgagca aattacctct ctcctgacag 120
ccctgttggg ggtgtcagtt gtggggttct ggtacctccc atcccggccc cagctagagc 180
aggcaggccc acaggaattt gaatctggag ggagttatca gtggtggagc gtagaggctg 240
caggaatctt catataagtg gagtcatata atatgtggtc ttttggtctg gcttctttac 300
ttagcatgct ttcaaggttc atccatgtcg tgggatatat tagtgcttca tttcacttct 360
gatggcaggg tcctgaatta agtcctgccc tgaggatcac actgttacat ctaggccttg 420
taggattcct gaaaattgca tcccatctgc cctgctctaa aatctacaca gcctttgcca 480
tatccattta tc 492

4

557

DNA

Homo sapiens

4
ctagggctta gaagtttcct taacaccaac tgaggagtcc cagggtggga gctgagctca 60
cacgaagctc tacttcgcgt gtcctgaagc tttgacagtt gggcctcttt ctggcttttg 120
catcctctgc tcatactaag gccagcagag ctacaggttg gaccatggtc tggccgggag 180
ctccagcttc ctcttttcct ctcagtagtc atcgggccag ctgcccatac ctggtgccca 240
ggtatgagaa gagacctttg gctttcacca ggttcttgag agggtcaggg acctccaaaa 300
ggataaatgc cattggtcag atagtcactg tctacctccc tcccatccat ttcctgcctt 360
caacccctgt tcttgggaca aggccttcgc tggcattcaa tggcgggtcc ctgaggaagg 420
ccccagcctt gccgaacatc cttcaaggga gacttcagcc cctggccctg cagtgagtgt 480
gttgttagca gcagaggggc tgagtgaggc agtgggatgg ggcttttctt ttttgttctg 540
gttgcaaatt ataaacc 557

5

819

DNA

Homo sapiens

5
gccttcagtt tgtcccgttc taaccgcccg gccgtctcaa tgcgatcaca ggagggacac 60
ggagatcgag gagggccaca gccggaccag gccaggcgtg ctgcggcagg aggaaggctc 120
cggaggtggg gtgggaaggg gaggccgaga gacgggtgtc gccgcgcccc cgctgccgcc 180
agagaggagc ctacggctgg cagcctggcc tgggcagcag ggtcctcggc gctcggctgg 240
gaaatcgcac gtctctccgc ggtgacctgt gcacagcccc tgggcctccg cctccgtgct 300
ggcagcctcc gcctcagcgc acaaagcccc gtcaccccga ctctcggagc gccgccgccg 360
ccaaatcctc agcccctccc tcattggccg cggcgtctgc cgggaagtgc agtcccgggt 420
ttggggcgat ggagcccagg aggaagcggc gagtcagcgc ggcggagagg gcggagggga 480
cggagggggc ggaaggggac gaaccacgaa cgcccgcggc cgcgaagggt ctggacgaca 540
aaggagagac tagcgagagg gcttgttgct tttttttttt tttttttttt tttttttttt 600
gagacggagt ctcgctctgt cacccaggct ggagtgcaat ggcgcgatct cggctcgctg 660
caacctccgc ctcccgggtt caagcgattc tcctgcctca gcctccgagg agctgggatt 720
acgggggcgc gcctccacgc ccagctaatt ctttgtactt ttagtagaga tgaggtttca 780
ccatgttggc caggctggta tcgaactcct gacctcgcg 819

6

581

DNA

Homo sapiens

6
ccctgccatt ctggatagtt ttggttattt ttagtttaac ttcaggtgag ccactcttct 60
ttggaatcac agtttgtggt accgaagtgg tggctgtgga ggggaagggt tttctagaat 120
ttaatgtggt cttctgttta gaatttctga atggtaatag tcagtggggc taaagtttct 180
agctgcgcca aagtcattgg ctgacccatt aggatattga ttatgcgact ggtatttggt 240
ttgaatttca tacatgctga ttgatggcag gtagccattt gtgagtggag gaagatctgt 300
tgtaggtgag tattgaaagc tttgctgcaa ggtagcttcg tatggtgtct ggccaccatc 360
ttcagcaatg tcacttttgt tatcaaaggc atcctcctga cggatgctgg cggagtcagt 420
gagttgaggt ggttgttgaa ctgtgtttcc cgtgatccct tgcatgaaag agaaagagaa 480
atccattgtt ctgctccagc attcttaact ttccctttct ctcatcgggc cttcagtttg 540
tcccgttcta accgcccggc cgtctcaatg cgatcacagg a 581

7

602

DNA

Homo sapiens

7
attccctaag agatttgtcc caagaacaca tgggttgcta cagcggagga gaaatcaagt 60
ctgttttccc tctggtgaat tgtcccctga acgtgctttc ttcctacagt gttgccagga 120
aagtaaaaaa aaaaaaaaaa aaaaaaaaaa agtccacgat gtcagctggg gtgataccaa 180
aacaattgtg ggaggaacaa catccgcaaa ttgaatagtg tgaggagtgt ggacagaaga 240
tgttttgtct ttggcctcat ctcccagact tgatctttgt aaatacagaa gtttccacca 300
gagccgaacc tggcaatgac ttgaggagca gctgcaagga agacagcctc tcccagggta 360
tcacctgggg gcacacccca gcttcccctc ctgagcctca tcgaggggtt agtgctacct 420
ctcgggaaaa cataaagatg acaagaagcc aaaggtgcca atagttccca tttagtataa 480
aagctggctc agcaaatcat gctatttcag ggcctagggt gggccagttc ccaggcagcc 540
ctggcaggaa ggactctgag aggcggacag taaggtaggg gcttgggagt gataggtcta 600
aa 602

8

572

DNA

Homo sapiens

8
gagaggacaa acattcaaac catggcagca gcgtttgtgg gaaacacgcg ccaaagcaag 60
aaaagaccaa cctaagagtg agtgacttgg ctcctcattc tacattcatt ttcatccaat 120
ggggcccaag ggcatgtacc cattacccat ctgggcagtt cccttgaatg tgggcttctg 180
tttgcccgtg gaggtgagga acttcaagga agaaaccatg aaagacctct tgaggctgag 240
ggctggcacc agcaccaaga tctccaggca gctggaacag tgatggctcc tccgtcctcg 300
caggcggggc acccaacagg gtgtgaccgt cacctgaggg gagacagcca gaggcacagg 360
cctgatcctg ggactgaggt tggcggtttg ggtggagagg tgattctgag tgtgacaccc 420
tccagtgata aagtgggggg cttcccagca gcccctgggg aaacaggctg catctctgga 480
gacaggagat atgtggaggc ctgaggggca gtggaaagcc ctgtgtgtct gggtcaggtc 540
tctccttggc aggtaatggt ttgtcccagt ag 572

9

606

DNA

Homo sapiens

9
gaagcttctc catgttctta gccaggttat ataacttctt ttgcttcttt cttttccaca 60
ttctacatat ttttcacaat gaggatgtgt cacttctata gttaaaaatg gaaatcttca 120
tttaaaagaa caatcagaca caaggcaagg tatgcttacc ttcccaatga ccaaggagca 180
gggagacatt ggtcgtggag gccataggtg accagccttg agggaaggga aggaagggga 240
ggtgcaggga ggcgcagcag aggcacccga gtgtgctcta cgaatgtaag tctgccagct 300
gctcctctgt gcctagcacc ctggaaagcg cccgcacaca gtgggccctc agcaaactcc 360
cactgagcaa agggccctgt gagtaaggac ctagaagcag ggtgtgcttg aggcatgggg 420
tgggagggca tccagcttgg ctggcggtgg gagccgatgc aaaggtgagc tgagaccaga 480
cggtagaaga ccttcagtgc tgggccgagg agggttcctt catcccatag agaagagctg 540
ccatccaaga cagaaggctg gggagtgaca tgttgaaatc agtatcttag gaaaataaag 600
ccactg 606

10

664

DNA

Homo sapiens

10
gccataaaat caggataatg gtggctgtgc ctccttagag tagaatgaga atcagagcag 60
aacaagggaa agctgcagtg acttgtcagg tgtcaacctt cagcatgatg ggagagccat 120
gggacccttc ccctttccct aagagagcca gccctcacag caggcctggg atccaatgcc 180
cagcacccag ctgggagccc agggaccttg gccaaaatct ggttctgctt caacttggtg 240
cccggccttt gtcaagtcac ttcctcattt gcaaactggg agagtttgga tgaaactatt 300
gaatgaaatt attttggggg tttctttctg gctctcacag tcctcgcatg ctcaccatgt 360
tcccttcaat ttcattagca cagcccaaca aagggttaag cagtggcagt tcctctcgtt 420
ctttggttag gacaggaagg tcaggggtga ggccaataca agaggtagcc gccacagctg 480
atgctggaaa tgacaatagt tctttcctag actcatattt gtcccctctc cctgaagctt 540
tgcctgcagt gcccttgtaa agaagttggc aagaagcagg agtgaggctc agcccctctc 600
tgaaatggat acgccggttg ctccccctca tggctggtct catttgcctt cttcattttt 660
agac 664

11

688

DNA

Homo sapiens

11
tgcctagaac attctggtat cccagacaga agtgcaacac caggtggagg tgttgcagct 60
gagaagttct gaccagcata ccagcacccc cttaaaagct gtctatactt gcggttctca 120
gctaggctgc cacagaatcg cccaagtctt aaaaaaacag gtctctgtcc tactgcttga 180
gatgctggtt ctctaggtct gaggttctgg cacctgtgtg tttttaaaac tttacaaata 240
atttaaaagc gccttcaagt ccacaacaac agaatatttc cctccactcc tcattgtcct 300
ggagttctct aacagtgcag ttctacaact ggacacacga tgtcgctttt cagccacagt 360
tctcactaag cggccccaca gggcggcagg tgccttctgc agagagagag agagggcctt 420
ggctgacagg ccaagaccgg gcatcctggc tcctcctctg tacagacttt tcacagatgt 480
gaactctccc tactccctgt cttctgcccc caaatgaagc ctctcagctg gcaagagctg 540
agaactacca agcgagccat tgctaatttc tattgtgttt ggaaccacaa aaggcagaat 600
tattaaggct gtaaaggacc tcagagcatc tggtgcagtg agtttccaac ttgtgaaaat 660
ctgatgtgat ctcggacaag tcacttaa 688

12

550

DNA

Homo sapiens

12
caaagtggct ctgattattt cactcccaca ggccactggc tcagaggtat agagctcacc 60
tgtggcagat ggagatgcgg atctgaggct tctgatgctg ccacacccag cggcgccccc 120
caaattccgg gcccctggat gacatctggt ctgttcctgc agcatcagag cacaatagag 180
ccagccacca gtcccagccc tgcctgcatc ccatccattc ctgggtgcct aaccccgagg 240
atcccctggc agtatgatgc ggacctgtct tggatcccag ggatatgctg gccacgggga 300
ggagccggaa accaaccttt gtgtcactgt gtagtgacaa gtgcctttgg aggtcacaat 360
agccagtggt gatttctacc actgccccca gcagccaagg tggcagagga gccctgtcag 420
tcacccccat tctgttcatg gtctcacggt gggctccaca tggggggtgg cagccctctc 480
ccccacccca cccgacccct ttcgacagat agggtaatac aaatacaaat aacaccaaaa 540
gattgagttg 550

13

531

DNA

Homo sapiens

13
ggatctccta cgtactgcta cgactgcaca caggtgcagg aaatggctgt ttgctttgcg 60
tttgaggaac ttggaaaggg agacgtggta gggaaagtag tggttagggg cactttcaga 120
ctgaaggatg tgggttgggg aatacgggat tcttggagtt gaggacgccg cttctctcgt 180
ctctagctaa tgtgagaaag acccttctgg acactaagcc tgcaattcca ctggtggcta 240
ccaggtgtcc gtggtgtcct ggggcgggtg taatgagagc gggagcctgt gaaaccaaaa 300
gcattgtttt tataaattca gcactcttca atccctatta ataaggttag cggtgcagtt 360
cttgcgtctc ctgccctgcc tcaccttgca atcatattca ttggcattcc tttcttccaa 420
gaacccacct aggaggcctt gcaggagata tctctggtgc tggctgttcc tgcagtctga 480
aaagccagtt aagatacaaa tatgtgagag gacactgctt gaatctgatt t 531

14

673

DNA

Homo sapiens

14
tgagggtaaa atgaaacaat aatcacgggt gcatcctgga gctctttctt acaaggcgtg 60
cccccaaatc tgtcccctct ttctgaggat gcccttcccc tattgtctcc ctggccattt 120
cctacccatt ctcaagggcc atgatctcag ggagttctcc tgactcaccc aggcatattg 180
gatctcctac gtactgctac gactgcacac aggtgcagga aatggctgtt tgctttgcgt 240
ttgaggaact tggaaaggga gacgtggtag ggaaagtagt ggttaggggc actttcagac 300
tgaaggatgt gggttgggga atacgggatt cttggagttg aggacgccgc ttctctcgtc 360
tctagctaat gtgagaaaga cccttctgga cactaagcct gcaattccac tggtggctac 420
caggtgtccg tggtgtcctg gggcgggtgt aatgagagcg ggagcctgtg aaaccaaaag 480
cattgttttt ataaattcag cactcttcaa tccctattaa taaggttagc ggtgcagttc 540
ttgcgtctcc tgccctgcct caccttgcaa tcatattcat tggcattcct ttcttccaag 600
aacccaccta ggaggccttg caggagatat ctctggtgct ggctgttcct gcagtctgaa 660
aagccagtta aga 673

15

570

DNA

Homo sapiens

15
aaagacactg cagagaaaag aaagcacagc ctgctgccct gggaattaac atgatttagg 60
agacctgcag gtcaccccct catgactaaa agccatcctg gaatgaaggt ctgtggctat 120
ttctaggcaa aactgtctga taagataaaa tagctcaact cctgaccatt aagtcgtgaa 180
ggccatggcc atcgtaaatc tcatctttcc ggccctctgg cctgcatgca gtgcagccca 240
gccagtcggt ggcagccacc ttggtaggaa gggccctcat cctcctggct gtgccccaag 300
gactgggcag gcttcggtgc caagggtagt gcgagcactt gaaagccgcc ctgtatgttt 360
attgttttcc ccaggtgatc cagaattact cccgaactct accagctgaa atcctcctca 420
actcacatca gacaagacgg ccctgccact tacctgtcag atcactttgg gcaggtaagc 480
tcattttcct gaatctttac ttccacacct taaaatgtga gcaatactat ctccctggca 540
aggttgtttg tgagggtaaa atgaaacaat 570

16

517

DNA

Homo sapiens

16
actcctagaa ccctaaagga agcccctgga cacgcaggaa ggtgtggaga ggagttctca 60
tacttgcact tgggaggagg gctcaggaga aacagaggct ggcaacaccc cctcacacac 120
tggtcctctg gagggccagt gtctacagac actgtggact gagtccacag agaggaaagg 180
gtcctgcctt catcagaact gctcagcaag cagttccatc ccagggggtc ctgcgaggta 240
agggaggggc agctagctag gtgaggggct gagagagtgg ggaggggaaa gagggaagaa 300
gagagtgaga gggagaggga gggactgagc cgattctcag ctccttgacc gtttgctgag 360
ctctgtctga gtggacagat ggtcccaagt caggccacac cagagtggcc tttctgctcc 420
cctacaccct gcattcctca acattgctgg ccccggagag actttccttc agagaagcaa 480
atggctgggg aatggtgaaa gacactgcag agaaaag 517

17

586

DNA

Homo sapiens

17
gtatcaccag tgaagttggt gcacacagca gggcccaaac tgacatctca cagcacccag 60
caggtcactg tgggcatgat aggatgatgg gtcactgtgc cagccctgaa ggagttcaag 120
tccagatagg ggaaggtggt ggaccagacc cagacagaga ttctgagtcg ctgctgagac 180
tgggtgaggg tagtgggtac atgggaggac atatagcccg gcagcccagg gctggagtcc 240
acactcaggt tggggcagcc tggtctgcct ctcctgcagg agacttttcc aggcaggctt 300
gtccctccag aatgcacgaa tcaaatcctc tcaggatcag tctcattttc ctcgtgctgg 360
gggagcaggc tactcacaga agatgttgtt gcaaatgtaa gaatcacatg tcgatccaca 420
aactggcatt gagcagctac ctaggagatc aaagaaactc ttactttggg agctcctgcc 480
agggctcttt gggaggtctg gctagctctg gaggaagaga atgaacttgg ggagggcgtg 540
gaacagatga ggacgcaggc actgccattc aaagaggaga ggtctc 586

18

1100

DNA

Homo sapiens

18
cacagctgga gacatgttac acttctgcaa atccaggctc cctttgtgca ttgtaatgga 60
agctggtagg atttccttgc tgccacagtt ttccaggcta ttttaacagg aggtggctct 120
tcctcgtccg cgcttgtgtg ctgcctcggg ctgtgtctcc aaatgtcagt acttgagagt 180
gaggaggcca cctcccctgc attgatctgt tctggctgag tttaaagcac agatcttggt 240
catcaggttt ttttaacttc ggctttggag acaacattct tttttttttt ttttttagat 300
ggagtctcgc tctgtcgccc aggctggaat gcagtggtgc gatctccggt tcatgccatt 360
ctcctgcctc agcctcccga gtacctggga ttacaggcgc ccaccaccat gcccggctaa 420
tttttttgta tttttttagt agagacgggg tttcaccgtg ttagccagga tggtctcaat 480
ctcctgacct tgtgatccgc ccgcctgggc ctcccaaagt gctgggatta cagacgtgag 540
ccaccgcgcc cggccccaac attctttttt gcttgggata aaccctcttc aggctgttaa 600
tcaatataga taaaagtata ctgttctatt ctttcttctc aagtcatttt caatgctttc 660
tctgcatggg caatgccaaa atcccattga gcatgttctc tctgaggtca gggtttccaa 720
atcttttgtt tctcagagtg attgctggct tgtttggttg cctcagccag taattcttca 780
taccgcttat gacctttata ctcctgtacc catttttcat gaacccacac cctctctggc 840
tgtttgctaa aacactggac atgatatttt cgggcacctc ctgtgttaat tttggtatga 900
acctccagct ggggatcact tcaatccata caaggccagc acggataggt tcccaccttg 960
gaccacacaa gatcaccaac ttgaaactta acaccagtgg acacttctgt tgttggaaca 1020
gaagatagta ttggctgaac tggggcttcc tcttttagta ctggatcttc ccttggtttt 1080
tctgatatgg tgtgaaccct 1100

19

1236

DNA

Homo sapiens

19
gagctgagat cgcaccactt cactccagcc tgggcaacag agcaagactc tgccttgaaa 60
aaaaaaaaaa aaaaagaacc cttgctctta tggagaagca ttctactggg gaaaacagac 120
aataaacaca tagataaata aaacatatgt cagaaagggg taagtgatgc tcagaaaaat 180
aggacaaggg atcagaagtg ctaggcgttg caatttaaaa taatggggtc tggaaagcca 240
ccctgagaag ggccaatgga gctatgccca gcctgtggta tccttcctca ggcaggggac 300
tgtaccccca gcttacaagc cttttcgagg caccttcagg gtttgagccc aaaatgcact 360
gcacattaag tgttattgat gggcttgtgg aaatctgctc tccgtcaggc tctgatgaaa 420
cttttccaac aggagactga actcagtact gggcatggcc ccctgcacat agtaagtcca 480
cagcaaatgt gtgtggggtg aatactgtct tctttatcct ccctttttct actccagtcc 540
aggtagacgg tgtatctacc cacccaattt tgctgtttct ggtaccctca gtgggtcttg 600
cctgctcttc ctccttgaaa tcattactca gaggtctccg tgtcagctcc agacagatgg 660
gcctggtgct cctttatctc aatacaattc ccccgctccc aggctccagg ggaaatcctg 720
agctaacttt ggtctttcct aaactccaca cttccctttg ggcacctctt tccccattgc 780
ggggctccct ttcagaattt gctgctttat ttatttattt atttttgtca gaggtgcatt 840
aatgatgctt tatttaaaaa caaaaaactt ggccaggcgc ggtggcttat gcctgtaatc 900
ccagcacttt gggaggctga ggtgggcgga tcacaaggtc aggagatcaa gaccatccag 960
accaacatgg tgaaacccag tctgtactaa aaatacaaaa aaaaaaaaaa aaaaaaatca 1020
gccgggcgtg gcacatgcct gtaattccag ctactcagga ggctgaggca ggagaatcac 1080
ttgaacccgg gaggcagagg ttgcagtgag ccgagattgg gccactgcac tccagcctgg 1140
gtgacagagc gagactccat ctcaaacaaa aaaacaccac aaataaataa aaaataaaaa 1200
caaaaaacca aaaacagtcc attccatgtc gtgttg 1236

20

1171

DNA

Homo sapiens

20
accgtgcaga ggtagatggt acttctcaca tcctgtaatt aattgcttgc atgcctgtgg 60
tacaccgtac cattgctcac aaatatttcc tcttcctctc tggagaagga ttacacttcc 120
ttgatccatt ggcctcactc aggcttcgcc acatgactgg tgctggccag tgaagtggga 180
catgtgagtt actggggagc agaaactttc aaagccaagc atggctcacc ttgttctctc 240
ttccctctgt tacaaggatg gcagtgcccc agccagaaac cacactgcca gcctggcata 300
ggagtggagc agggcacaca taagtgtgag agaaatgaac ttcacctcag tgctcgaggc 360
ccctgggacc atggactcat ttgttactgc agcataaact agctggtctt gacttcagct 420
tctgcctctc ttccctggct ttgccttcac aaaatcaaga gtctgatctt actcatatta 480
tagagctggt cttcaaccat gttctttttt tttttttcct tttttttttt ttgagatgga 540
gtttcgctca tgtagtccag gatggagtgc aatggtgcga tctgggctca ctgcaatctc 600
tgcctccctg gttcaagcga ttctcctgcc tcagcctccc aaatggctgg gattacaggt 660
gcttgccacc acgcctagct aatttttgta tttttagaag agataggatt tcaccatgtt 720
ggccaggcta tctcaaactc ctgacctcag gagatctgcc cgcttcggcc tcccaaagtg 780
ctggcattac aggcatgagc caccatgccc agccccatgt tcttcgaatg gccaagaata 840
tcagtaggta aggtttgttg agcatggatt gtgtgccagg tgctgtgcta tgcctttggc 900
aagcgttatc ccattacatc ctttaacagc actctctggt gggtactgct cttatacaag 960
atttacaaaa gagaaaagag actcagagga atgtaggggc tcacgtgaga ttgtaaagta 1020
gtgagaagtg gagctggtcc ttcagctcaa atccctctga tgccaaagaa ctcaaactct 1080
gcagttccat catgtgcatc ctcactctct cacttcctgc agaggatctg tggaggttgt 1140
aactattgtt ttcacctgtc tcttccactg g 1171

21

1007

DNA

Homo sapiens

21
acggtgtaca catttggtta gttccttctt ttacaccata aattatcaga gacaaccctt 60
ttggaaagca atttggcaat aatatcaagt gacataaaga tgttcatagc ctttggccca 120
ataatcttcc tcctgggaat taatcctaag aaaataattc acaaggaaga aagaaccatt 180
ttgtatctac aaagacattt attggggtgt tatttatgat agggaaaaac tggagacacc 240
ctccccagcc aacatagagg gatggtccta taaattatga tacatccatc caatgaaatg 300
ttaccctgcc agtaaaaatg gtaaattgaa aattgtgtag caacaagaaa gagtgttcag 360
aaaataaaag caagtgaaaa aaggagcaca ggattgtcta tatgctgtga tcaatggcaa 420
tgctgacaaa tccatgtatg tgtagatagt tgtagtgtta cctgtaaggc acgataaggc 480
ggatgtaact gtgcttcaaa tgttcttttc catagctgtg gagtccttct agtatgaaat 540
catttttgta aaatcgcaat ttgtcaccag gggttttgac tttccttatt gcctcgtggg 600
aggtggcaga ggcagcacat ctcagaccga gcctcggttc ctccccctac ctcccaccgc 660
tgcgcttgaa gaaagatgct gcagcctccc cagtccccat gccagcgctc ccacttttct 720
ctgagctttc ggtggcagac agcgccttgg gcactttttc atgctcataa ttcgaattac 780
ctgtttaagt cggtcaaatg aaaaaatacc agctccgccc ccacgcgggc tggccggggc 840
gcctggagcg ccagggcggc tgcagcgcgc tctccgcggc cgtcggccct gagctcattt 900
cctggggcgc gcgcgccggg ctatttcagc ctggcgctgt gcaaacagga caatttactg 960
cggccaaaag ggacccaaat tacaatcgta tcacagacaa atatccg 1007

22

198

DNA

Homo sapiens

22
ggctggagca gggacttgaa aaagggagag ggctcaggag actcagagga ggaggaaagt 60
gtgtgagcag taggcagggt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgcacgc 120
gcgcgcatgc aggcctgtgt agggctggga aagaacaaag caaaagggtg cacaaggcat 180
ccagaagcca gggcaatg 198

23

369

DNA

Homo sapiens

23
gagcagaagg ccttgactga gccccaggag aggcaggaca ccaggggtgc acacccataa 60
acacacacat acacatgtat gtctcctccc tggagcctga gagtccctat atacagcagg 120
tgcatgtggg ccacacatca cacaaaattg aatacaggca ggctcagagc accagcacac 180
acgtatgtcc ttgacaccct tagagatact actaagcacg tgtgtgtacc tgctcaccca 240
tatggcagag cccctggatc tgggcagaaa tgccaaagca ggggcaggcg cgtgtgcgcg 300
cacacacaca cacacacaca cacacacaca ctagcacagc cacaaaagct caatccacat 360
ccagcattc 369

24

436

DNA

Homo sapiens

misc_feature

121,153

n = A,T,C or G

24
tcaggacgtt gcactttgac agaaggctct ggaaggaaac tttaaaggga gccttccaga 60
gggaaatgcg gtgttggggt aggtctgcct ttggctatgg gctttctggc tgccggaggg 120
ncccagggtc ccccaggaaa gccttctgtg ganggtcttt tgagagagac aaagcagagg 180
ggtggaggaa gggcggctca ggtggaagga gtgaggacaa aggtgagtgc ccctgggcag 240
gaagtgctga aagagagaag gagggaggcc accaggcctg ggcctggagc cagcctggga 300
gactcccagc cgcccacttc tcggggcctc ccttttccag ccccttgctt tcgaggcagc 360
agtgccatta tttggggaaa ccagctaacc agataggaca gcaaaccggg gatttatgtg 420
gtgtgggaac agctca 436

25

670

DNA

Homo sapiens

25
ctgggattac aggtgtctgg caccatacct agctaatttt tgtattttta gtagagatgg 60
ggtcttgcca tgttggccag actggtttcc aactccccac ctcaggtgat ctgcccgccc 120
tggcctccca aagtgctggg attacgggca tgagccactg tgcctgacct cagctctgtt 180
attaataagc taaatggctt tgagcgactt gccttatcac ttgagcctca gtttcctcat 240
ctgtaaaatg gggataaact tcttccgtcc gcatgaggat gctgagagac gtgagtgagg 300
tggtctatga aagctcttgt catagcctgg catgcagggg taacatctgg atgatgaaga 360
tgatgatacc tgagattttt gccttacaga caactccaga gagccctgtg aaatatttat 420
atgccactga acagggcaca agatgaagcc attagcctgc gcttacatag tagaatgtgt 480
gaatcagatg agatgcttgg tctctagtaa gaccttaagg gatggacaga agacaggcag 540
attttggata tggtatacgt ggctgtgggc tagcgtgttt actactgggc ctgggatgta 600
tttggaatgt acacatgtgt cctttgcttc tcagaacact ttgaggcagc agagttacta 660
ctgcctgcca 670

26

460

DNA

Homo sapiens

26
gaggttagac gggtctgagt tcaagcccca ggtctgccac ttcctggctg tgtgccctgg 60
aacaagtcac cttatctctc tgaacttctt tttttttttt ttctttttga gatggagtct 120
cgttctgtcg cccaggctgg agtgcagtgg catgatctca gctcactgca agctccgcct 180
cccgggctca cgccattctc ttgcctcagc ctcccgagta gctgggacta caagcacccg 240
ccaccacgcc cggctaattt tttgtatttt ttagtagaga cggggtttca ccgtgttagc 300
caggatggtc tcaatctcct gacctcgtga tccgcccgcc tcggcctccc aaagtgctgg 360
gattacaggc atgagccacc atgcctggcc atctctctga acttctgttt cctcatctga 420
gatgacagtc agagtgggat ctgtgtaagg cactttgcac 460

27

482

DNA

Homo sapiens

27
tgccaattag ccacactctt ctctagagag gtttcaagtc atttttcttc acagcaatgg 60
caaggctctt aaataaggtc cttgaagttt cttcgggtct cctcccacct gctccctgcc 120
cccttcacct ccacccacct gcttcccttt ctcatccccc agaggcggag gcttccgagg 180
aatttggggt agggaaatag gaatcagggg ctcctcattc cccaaaggag cctctttggc 240
aagcaggcac gtgggtctcc gggctggtgc acatcacagg gcagccagcc caagtgccat 300
cttgatgccc aatcagtctt cctcatggct gcgccctgct ggtctctcag agggttaatg 360
caatttcttg gaggacgaca ttcctaacac ccaggggcca gaactccttc cccactggtt 420
attcccatgg cccagagcag caggatgggg gcagaaacag gcatggacct taacagcagc 480
gt 482

28

16

DNA

Artificial Sequence

PCR primer

28
attggcctgg cttctg 16

29

16

DNA

Artificial Sequence

PCR primer

29
ggctggagca gggact 16

30

20

DNA

Artificial Sequence

PCR primer

30
tcctgtgatc gcattgagac 20

31

21

DNA

Artificial Sequence

PCR primer

31
ccctgccatt ctggatagtt t 21

32

20

DNA

Artificial Sequence

PCR primer

32
cagtggcttt attttcctaa 20

33

20

DNA

Artificial Sequence

PCR primer

33
gaagcttctc catgttctta 20

34

20

DNA

Artificial Sequence

PCR primer

34
gtctaaaaat gaagaaggca 20

35

20

DNA

Artificial Sequence

PCR primer

35
ttaagtgact tgtccgagat 20

36

20

DNA

Artificial Sequence

PCR primer

36
caactcaatc ttttggtgtt 20

37

20

DNA

Artificial Sequence

PCR primer

37
aaatcagatt caagcagtgt 20

38

20

DNA

Artificial Sequence

PCR primer

38
tcttaactgg cttttcagac 20

39

20

DNA

Artificial Sequence

PCR primer

39
attgtttcat tttaccctca 20

40

20

DNA

Artificial Sequence

PCR primer

40
gccataaaat caggataatg 20

41

20

DNA

Artificial Sequence

PCR primer

41
tgcctagaac attctggtat 20

42

20

DNA

Artificial Sequence

PCR primer

42
caaagtggct ctgattattt 20

43

20

DNA

Artificial Sequence

PCR primer

43
ggatctccta cgtactgcta 20

44

20

DNA

Artificial Sequence

PCR primer

44
tgagggtaaa atgaaacaat 20

45

20

DNA

Artificial Sequence

PCR primer

45
aaagacactg cagagaaaag 20

46

20

DNA

Artificial Sequence

PCR primer

46
cttttctctg cagtgtcttt 20

47

20

DNA

Artificial Sequence

PCR primer

47
gagacctctc ctctttgaat 20

48

20

DNA

Artificial Sequence

PCR primer

48
ggtttcaact agttctggtg 20

49

20

DNA

Artificial Sequence

PCR primer

49
ctagggctta gaagtttcct 20

50

20

DNA

Artificial Sequence

PCR primer

50
actcctagaa ccctaaagga 20

51

20

DNA

Artificial Sequence

PCR primer

51
gtatcaccag tgaagttggt 20

52

20

DNA

Artificial Sequence

PCR primer

52
gataaatgga tatggcaaag 20

53

20

DNA

Artificial Sequence

PCR primer

53
ggtttataat ttgcaaccag 20

54

20

DNA

Artificial Sequence

PCR primer

54
attccctaag agatttgtcc 20

55

20

DNA

Artificial Sequence

PCR primer

55
gagaggacaa acattcaaac 20

56

20

DNA

Artificial Sequence

PCR primer

56
tttagaccta tcactcccaa 20

57

20

DNA

Artificial Sequence

PCR primer

57
ctactgggac aaaccattac 20

58

20

DNA

Artificial Sequence

PCR primer

58
agagagggtg agtaacttcc 20

59

20

DNA

Artificial Sequence

PCR primer

59
aataaaagaa agtttggggt 20

60

20

DNA

Artificial Sequence

PCR primer

60
gcagagtgct tttagaacat 20

61

20

DNA

Artificial Sequence

PCR primer

61
aggtggaggt tacagtaaga 20

62

20

DNA

Artificial Sequence

PCR primer

62
aagcagtatc tctgaagctg 20

63

20

DNA

Artificial Sequence

PCR primer

63
ccttttcttg gttcagataa 20

64

21

DNA

Artificial Sequence

PCR primer

64
acggtgtaca catttggtta g 21

65

21

DNA

Artificial Sequence

PCR primer

65
cggatatttg tctgtgatac g 21

66

21

DNA

Artificial Sequence

PCR primer

66
accgtgcaga ggtagatggt a 21

67

20

DNA

Artificial Sequence

PCR primer

67
ccagtggaag agacaggtga 20

68

20

DNA

Artificial Sequence

PCR primer

68
gagctgagat cgcaccactt 20

69

21

DNA

Artificial Sequence

PCR primer

69
caacacgaca tggaatggac t 21

70

21

DNA

Artificial Sequence

PCR primer

70
cacagctgga gacatgttac a 21

71

21

DNA

Artificial Sequence

PCR primer

71
agggttcaca ccatatcaga a 21

72

19

DNA

Artificial Sequence

PCR primer

72
acgctgctgt taaggtcca 19

73

21

DNA

Artificial Sequence

PCR primer

73
tgccaattag ccacactctt c 21

74

20

DNA

Artificial Sequence

PCR primer

74
gtgcaaagtg ccttacacag 20

75

21

DNA

Artificial Sequence

PCR primer

75
gaggttagac gggtctgagt t 21

76

21

DNA

Artificial Sequence

PCR primer

76
tggcaggcag tagtaactct g 21

77

20

DNA

Artificial Sequence

PCR primer

77
ctgggattac aggtgtctgg 20

78

21

DNA

Artificial Sequence

PCR primer

78
tgagctgttc ccacaccaca t 21

79

21

DNA

Artificial Sequence

PCR primer

79
tcaggacgtt gcactttgac a 21

80

21

DNA

Artificial Sequence

PCR primer

80
gaatgctgga tgtggattga g 21

81

20

DNA

Artificial Sequence

PCR primer

81
gagcagaagg ccttgactga 20

82

21

DNA

Artificial Sequence

PCR primer

82
gccttcagtt tgtcccgttc t 21

83

20

DNA

Artificial Sequence

PCR primer

83
cgcgaggtca ggagttcgat 20

84

21

DNA

Artificial Sequence

PCR primer

84
ccactgaaca gggcacaaga t 21

85

21

DNA

Artificial Sequence

PCR primer

85
gaattactgg ctgaggcaac c 21

86

17

DNA

Artificial Sequence

PCR primer

86
gcctgcgctt acatagt 17

87

21

DNA

Artificial Sequence

PCR primer

87
aggcgggcgg atcacaaggt c 21

88

22

DNA

Artificial Sequence

PCR primer

88
ggctcactgc aacctccacc ta 22

89

24

DNA

Artificial Sequence

PCR primer

89
aaccaaacaa gccagcaatc actc 24

90

22

DNA

Artificial Sequence

PCR primer

90
tgggaggctg aggcaagaga at 22

91

18

DNA

Artificial Sequence

PCR primer

91
actgggcctg ggatgtat 18

92

19

DNA

Artificial Sequence

PCR primer

92
aaaaattggc tgggtgtgg 19

93

22

DNA

Artificial Sequence

PCR primer

93
caggctggag tgcaatggtg tg 22

94

22

DNA

Artificial Sequence

PCR primer

94
tgggaggctg aggcaagaga at 22

95

20

DNA

Artificial Sequence

PCR primer

95
ctcactgcag cctcaactcg 20

96

24

DNA

Artificial Sequence

PCR primer

96
tagcagaagg gggaaggggg aacg 24

97

24

DNA

Artificial Sequence

PCR primer

97
gacggtgtac acatttggtt agtt 24

98

24

DNA

Artificial Sequence

PCR primer

98
gcacagttac atccgcctta tcgt 24

99

24

DNA

Artificial Sequence

PCR primer

99
tgcagacacc cagagaagac gact 24

100

22

DNA

Artificial Sequence

PCR primer

100
tggaccctgc cctcacgatg ga 22

101

56

DNA

Homo sapiens

101
tgggattaca ggtgcatgct accatgcccg gctaattttt ttgtattttt ttagta 56

102

16

DNA

Homo sapiens

102
accatgcccg gctaat 16

103

21

DNA

Homo sapiens

103
ctaccatgcc cggctaattt t 21

104

21

DNA

Artificial Sequence

PCR primer

104
tactactggg cctgggatgt a 21

105

22

DNA

Artificial Sequence

PCR primer

105
tagagaaaga cctcgttatt gg 22

	Number	Date	Country
	60/303386	Jul 2001	US
	60/221855	Jul 2000	US

Methods for identifying genomic deletions

Information

Patent Number

Date Filed

Date Issued

Inventors

Original Assignees

Examiners

Agents

CPC

US Classifications

Field of Search

US

International Classifications

Abstract

Description

Claims

CROSS-REFERENCE TO RELATED APPLICATION

Non-Patent Literature Citations (8)

Provisional Applications (2)

Entry
Keinlinen, M. et al., “Use of Polymerase Chain Reaction to Detect Heterozygous Familial Hypercholesterolemia”, Clin. Chemistry, vol. 36, pp. 900-903 (1990).*
Balemans et al., “Localization of the Gene for Van Buchem Disease to a Candidate Region of Less than 1 cM on Chromosome 17,” American Journal of Medicine 61 (suppl.):A12, 1997.
Balemans et al., “Localization of the Gene for Sclerosteosis to the Van Buchem Disease-Gene Region on Chromosome 17q12-q21,” American Journal of Medicine 64:1661-1669, 1999.
Beighton et al., “The Syndromic Status of Sclerosteosis and Van Buchem Disease,” Clinical Genetetics 25:175-181, 1984.
Brunkow et al., “Bone Dysplasia Sclerosteosis Results from Loss of the SOST Gene Product, a Novel Cystine Knot-Containing Protein,” Am. J. Hum. Genet. 68:577-589, 2001.
Burge et al., “Prediction of Complete Gene Structures in Human Genomic DNA,” J. Mol. Biol. 268:78-94, 1997.
Fryns et al., “Facial Paralysis at the Age of 2 Months as a First Clinical Sign of Van Buchem Disease (Endosteal Hyperostosis),” European Journal of Pediatrics 147:99-100, 1988.
Van Buchem et al., “Hyperostosis Corticalis Generalisata,” American Journal of Medicine 33:387-397, 1962.