Methods for Identifying Risk of Low Bmd and Treatments Thereof

Abstract

Provided herein are methods for identifying a risk of low BMD in a subject, reagents and kits for carrying out the methods, methods for identifying candidate therapeutics for treating low BMD-related disorders, such as osteoporosis, and therapeutic and preventative methods applicable to osteoporosis. These embodiments are based upon an analysis of polymorphic variations in nucleotide sequences within the human genome.

Description

FIELD OF THE INVENTION

The invention relates to genetic methods for identifying susceptibility to low bone mineral density (BMD) and/or bone damage generally associated with human diseases, and in particular to osteoporosis, and treatments that specifically target the disease.

BACKGROUND

Osteoporosis is a common disease characterized by low bone mineral density (BMD), deterioration of bone micro-architecture and increased risk of bone damage, such as fracture. Common types of osteoporosis include postmenopausal and senile osteoporosis, which generally occur later in life, e.g., 70+ years.

Osteoporosis is a major public health problem which affects quality of life and increases costs to health care providers. It is estimated that 44 million Americans and 100 million people worldwide are at risk for osteoporosis. In the United States today, 10 million individuals are estimated to already have the disease and almost 34 million more, or 55% of the people 50 years of age and older, have low bone mass, which puts them at increased risk of developing osteoporosis and related fractures. Of the 10 million Americans estimated to have osteoporosis, eight million are women and 2 million are men. These numbers are growing as the elderly population increases. It is estimated that by the middle of the next century the number of osteoporosis sufferers will double in the West, but may increase six-fold in Asia and South America. The estimated national direct expenditures (e.g., hospitals and nursing homes) for osteoporotic and associated fractures was $17 billion in 2001 ($47 million each day)—and the cost is rising (See National Osteoporosis Foundation; http://www.nof.org/osteoporosis/stats.htm).

Fracture is the most serious endpoint of osteoporosis, particularly fracture of the hip which affects up to 1.7 million people worldwide each year. One in two women and one in four men over age 50 will have an osteoporosis-related fracture in their lifetime. It is estimated that by the year 2050, the number of hip fractures worldwide will increase to over 6 million, as life expectancy and age of the population increase (See Spangler et al. “The Genetic Component of Osteoporosis Mini-review”; http:www.csa.com.osteointro.html).

Peak bone mass is mainly genetically determined, though dietary factors and physical activity can have positive effects. Peak bone mass occurs when skeletal growth ceases, after which time bone loss starts. In contrast to the positive balance that occurs during growth, in osteoporosis, the resorbed cavity is not completely refilled by bone and BMD decreases. Based on studies of family histories, twin studies, and racial factors, some attribute 50-60% of total bone variation (e.g., Bone Mineral Density to genetic effects and suggest there may be a predisposition for osteoporosis.

Osteoporosis can be considered a complex genetic trait with variants of several genes underlying the genetic determination of the variability of the phenotype. Low BMD is an important risk factor for fractures, the clinically most relevant feature of osteoporosis. Segregation analysis in families has shown that BMD is under polygenic control. In addition, biochemical markers of bone turnover have shown to have strong genetic components. Several candidate genes have been analyzed in relation to BMD, but the most widely studied gene in this respect, the vitamin D receptor (VDR) gene, explains only a small part of the genetic effect on BMD. Numerous studies, focusing on the BsmI allele of the vitamin D receptor gene have concluded that absence of the restriction site correlates with low bone mineral density.

SUMMARY

It has been discovered that certain polymorphic variations in human genomic DNA are associated with the occurrence of low bone mineral density (BMD) and/or bone damage generally associated with human diseases, and in particular to osteoporosis. In particular, polymorphic variants in loci containing CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1/TNIP1 regions in human genomic DNA have been associated with risk of low BMD.

Thus, featured herein are methods for identifying a subject at risk of low bone mineral density (BMD) and/or bone fracture, which indicates bone damage and related conditions such as osteoporosis in a subject. The methods comprise detecting the presence or absence of one or more of the polymorphic variations described herein in a human nucleic acid sample. In an embodiment, two or more polymorphic variations are detected and in some embodiments, 3 or more, or 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20 or more polymorphic variants are detected.

Also featured are nucleic acids that include one or more polymorphic variations associated with occurrence of low BMD, as well as polypeptides encoded by these nucleic acids. In addition, provided are methods for identifying candidate therapeutic molecules for osteoporosis and other low BMD-related disorders, as well as methods for treating osteoporosis in a subject by identifying a subject at risk of low BMD and treating the subject with a suitable prophylactic, treatment or therapeutic molecule.

Also provided are compositions comprising a cell from a subject suffering from low BMD or at risk of low BMD, and a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1/TNIP1 nucleic acid, with a RNAi, siRNA, antisense DNA or RNA, or ribozyme nucleic acid designed from a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence, or a nucleic acid that hybridizes to such a nucleotide sequence under stringent conditions. In an embodiment, the RNAi, siRNA, antisense DNA or RNA, or ribozyme nucleic acid is designed from a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence that includes one or more low BMD associated polymorphic variations, and in some instances, specifically interacts with such a nucleotide sequence. Further, provided are arrays of nucleic acids bound to a solid surface, in which one or more nucleic acid molecules of the array have a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence, or a fragment or substantially identical nucleic acid thereof, or a complementary nucleic acid of the foregoing. Featured also are compositions comprising a cell from a subject having low BMD or at risk of low BMD and/or a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 polypeptide, with an antibody that specifically binds to the polypeptide. In an embodiment, the antibody specifically binds to an epitope in the polypeptide that includes a non-synonymous amino acid modification associated with low BMD (e.g., results in an amino acid substitution in the encoded polypeptide associated with low BMD). In an embodiment, the antibody specifically binds to an epitope comprising an arginine corresponding to position 120 in a PROL4 polypeptide (SEQ ID NO: 12).

BRIEF DESCRIPTION OF THE DRAWINGS

FIGS. 1-4 show the position of each SNP in the chromosome on the x-axis, while the y-axis provides the negative logarithm of the p-value comparing the estimated allele frequency in the cases to that of the control group. Also shown in the figures are exons and introns of the genes in approximate chromosomal positions. More specifically, FIG. 1 shows proximal SNPs in a CETP region in genomic DNA. FIG. 2 shows proximal SNPs in a PROW region in genomic DNA. FIG. 3 shows proximal SNPs in a GRID2 region in genomic DNA. FIG. 4 shows proximal SNPs in a PDE4D region in genomic DNA. FIG. 5 shows proximal SNPs in a GPX3/TNIP1 region in genomic DNA.

DETAILED DESCRIPTION

It has been discovered that polymorphic variants described in a CETP, PROL4, GRID2, PDE4D and GPX3/TNIP1 loci in human genomic DNA are associated with occurrence of low BMD in subjects. Thus, detecting genetic determinants in and around this locus associated with an increased risk of low BMD occurrence can lead to early identification of a risk of low BMD, or its associated disorders such as osteoporosis, and early application of preventative and treatment measures. Associating the polymorphic variants with low BMD also has provided new targets for diagnosing low BMD, for prognosing osteoporosis, and methods for screening molecules useful in osteoporosis treatments and osteoporosis preventatives.

Cholestery ester transfer protein (CETP) transfers cholesteryl esters between lipoproteins. The transfer of insoluble cholesteryl esters among lipoprotein particles (HDL to LDL) by CETP is a step in normal cholesterol homeostasis. The lipoprotein phenotype of CETP deficiency, which is characterized by increased levels of HDL and decreased levels of low density lipoprotein (LDL), appears to have antiatherogenic potential. Bone mineral density is reduced by atherogenic diets (increased LDL/HDL ratios), possibly as a result of a shift in the balance of bone marrow stromal cells away from osteoblasts and towards adipocytes, which could result in reduced bone synthesis during remodeling. Lipoproteins may directly regulate bone density via the LRP5, the low-density lipoprotein receptor related protein 5. LRP5 was cloned from an osteoblast cDNA library (Dong, et al.: Biochem Biophys Res Commun. 1998 Oct. 29; 251(3):784-90) and mouse knockouts show a reduced BMD phenotype (Kato et. al.: J Cell Biol. 2002 Apr. 15; 157(2):303-14). A G171V mutation in LRP5 resulted in increased numbers of osteoblasts, increased AP activity, and increased trabecular number and thickness in mice (Babij et al.: J Bone Miner Res. 2003 June; 18(6): 960-74). The human G171V mutation was found to have the same phenotype, presumably by increasing WNT signaling in osteoblasts (Mao et. al.: Mol Cell. 2001 April; 7(4):801-9). Overall, there is evidence that lipoprotein profiles play a role in osteoblast differentiation. Provided is a method for treating osteoporosis or low bone mineral density by modulating a CETP function in a subject suffering from osteoporosis or low bone mineral density. CETP inhibitors and methods of making them are described in U.S. Pat. No. 6,586,613 (Substituted tetrahydronaphthaline and analogous compounds); U.S. Pat. No. 6,562,976 (4-phenyltetrahydrochinoline utilized as an inhibitor of the cholesterol ester transfer protein); U.S. Pat. No. 6,387,929 (4-heteroaryl-tetrahydroquinolines and their use as inhibitors of the cholesterin-ester transfer protein); U.S. Pat. No. 6,291,477 (Tetrahydroquinolines, processes for their preparation, pharmaceutical compositions containing them, and their use to prevent or treat hyperlipoproteinaemia); U.S. Pat. No. 6,218,431 (Substituted biphenyls); U.S. Pat. No. 6,207,671 (Cycloalkano-pyridines); U.S. Pat. No. 6,127,383 (2-aryl-substituted pyridines); U.S. Pat. No. 6,121,330 (5-Hydroxyalkyl substituted phenyls and their use in medicaments for the treatment of arteriosclerosis and hyperlipoproteinaemia); U.S. Pat. No. 6,069,148 (Cycloalkano-pyridines); U.S. Pat. No. 6,063,788 (Bicyclic-fused pyridines); U.S. Pat. No. 5,932,587 (Heterocyclic-fused pyridines); U.S. Pat. No. 5,925,645 (2-aryl-substituted pyridines); U.S. Pat. No. 6,753,346 (CETP activity inhibitor); U.S. Pat. No. 6,706,881 (Methods for preparing CETP inhibitors); U.S. Pat. No. 6,689,897 (Intermediates of CETP inhibitors); U.S. Pat. No. 6,600,045 (Methods for preparing CETP inhibitors); U.S. Pat. No. 6,573,383 (Preparation of anhydrous CETP inhibitor); U.S. Pat. No. 6,555,113 (Modulation of cholesteryl ester transfer protein (CETP) activity); U.S. Pat. No. 6,426,365 (CETP activity inhibitors); U.S. Pat. No. 6,410,022 (Modulation of cholesteryl ester transfer protein (CETP) activity); U.S. Pat. No. 6,410,020 (Monoclonal antibody reactive to human CETP and assay method for human CETP); U.S. Pat. No. 6,140,474 (Monoclonal antibody reactive with human-origin CETP and method of quantifying human-origin CETP); U.S. Pat. No. 5,948,435 (Methods of regulating CETP genes, enzymes and other compound, and pharmaceutical composition therefor); U.S. Pat. No. 5,519,001 (CETP inhibitor polypeptide antibodies against the synthetic polypeptide and prophylactic and therapeutic anti-atherosclerosis treatments); and U.S. Pat. No. 5,512,548 (CETP inhibitor polypeptide, antibodies against the synthetic polypeptide and prophylactic and therapeutic anti-atherosclerosis treatments).

PROL4, also known as Lacrimal proline rich protein (LPRP) is a member of the proline-rich secreted protein family and contains a conserved acidic N-terminal region. It has 45.5% amino acid homology to a salivary Parotid acidic protein (PRH1). PRH1 and related proline-rich (salivary) proteins act as potent inhibitors of hydroxyapatite crystal growth and bind calcium with a strength that suggests that they are important in maintaining the concentration of ionic calcium in saliva. The N-terminal region of salivary proline-rich proteins, which is also the region of strongest homology to PROL4, mediates this binding. It is expected that PROL4 plays a role in maintaining calcium levels during bone remodeling, and that a loss of function of PROL4 facilitates bone loss. Provided is a method of treating low BMD or osteoporosis by increasing expression of PROL4 or otherwise increasing levels of active PROL4 in a patient suffering from low BMD or osteoporosis.

Human glutamate receptor delta-2 (GRID2) is a member of the family of ionotropic glutamate receptors that are excitatory neurotransmitter receptors in mammalian brain. A point mutation in mouse GRID2, associated with the phenotype named “lurcher” in the heterozygous state, leads to ataxia resulting from selective, cell-autonomous apoptosis of cerebellar Purkinje cells during postnatal development. Mice homozygous for this mutation die shortly after birth from massive loss of mid- and hindbrain neurons during late embryogenesis. Glutamate receptors have been detected in osteoblasts and osteoclasts and may regulate bone resorption. Provided is a method of treating low bone mineral density or osteoporosis by modulating GRID2 function in a patient suffering from low BMD or osteoporosis.

PDE4D encodes cyclic AMP-dependent phosphodiesterase 4D. Phosphodiesterases are a superfamily of enzymes involved in degradation of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) (Manganiello V C, et al.: Arch Biochem Biophys 1995, 322(1):1-13; and Beavo J A: Physiol Rev 1995, 75(4):725-748). cAMP and cGMP are important second messengers participating in the response of various cells to hormones. In osteoblasts, cAMP produced in response to parathyroid hormone or prostaglandins regulates osteoblastic differentiation (Farndale R W, et al. Biochem J 1988, 252(1):263-268; Kumegawa M, et al. Calcif Tissue Int 1984, 36(1):72-76; Ishizuya T, et al. J Clin Invest 1997, 99(12):2961-2970; and Partridge N C, et al. J Cell Biochem 1994, 55(3):321-327), which leads to increases in cancellous bone volume as indicated by experiments in animal models (Jee W S, et al. Bone 1987, 8(3):171-178; High W B: et al. Bone 1987, 8(6):363-373; Reeve J: et al. J Bone Miner Res 1996, 11(4):440-445; and Finkelstein J S, et al. N Engl J Med 1994, 331(24): 1618-1623). Intracellular levels of cAMP are regulated by G protein-coupled adenylyl cyclase (Casperson G F, Bourne H R: Annu Rev Pharmacol Toxicol 1987, 27:371-384), and degradation is mediated by the phosphodiesterases. The phosphodiesterase superfamily consists of seven families, PDE1-7, distinguished by substrate specificity, chromatographic behavior during purification, and affinity for biochemical activators and inhibitors. Of these, the PDE4 family is specific for cAMP and is selectively inhibited by rolipram. Four PDE4 genes, 4A, 4B, 4C, and 4D, have been cloned from rat and humans, all of which are predicted to have multiple protein products due to alternate spicing of RNAs. PDE4 inhibitors have been shown to increase bone formation in normal mice (Kinoshita T, et al. Bone 2000, 27(6):811-817) and to ameliorate loss of bone mass in animal models of osteopenia (Miyamoto K, et al. Biochem Pharmacol 1997, 54(5):613-617; and Waki Y, et al. Jpn J Pharmacol 1999, 79(4):477-483). PDE4A and PDE4D are expressed in two common mouse osteoblastic cell lines, ST2 and MC3T3-E1, that represent different stages in the osteoblast differentiation pathway (Wakabayashi S, et al. J Bone Miner Res 2002, 17(2):249-256). PDE4 inhibition with rolipram increased BMP2-induced alkaline phosphatase activity, a marker of early osteoblast differentiation in ST2 cells. Furthermore, rolipram increased the expression of alkalic phosphatase, osteopontin, collagen type I and osteocalcin in the same osteoblast precursor cells (Wakabayashi S, et al. J Bone Miner Res 2002, 17(2):249-256).

Provided herein is a method for treating osteoporosis or low bone mineral density by modulating a PDE4 function in a human suffering from osteoporosis or low bone mineral density. Modulation of PDE4D by PDE4D inhibitors may increase bone formation and treat an osteoporotic condition. PDE4D inhibitors and methods of making them are described in the following U.S. Pat. Nos. 6,218,400 (Treatment method using a cGMP-Specific PDE inhibitor), 5,891,896 (Tri-substituted phenyl derivatives useful as PDE IV inhibitors), 5,849,770 (Tri-substituted phenyl derivatives useful as PDE IV inhibitors), 5,712,298 (Fluoroalkoxy-substituted benzamides and their use as cyclic nucleotide phosphodiesterase inhibitors), 5,491,147 (Tri-substituted phenyl derivatives and their use in pharmaceutical compositions and methods of treatment). Also provided is a method of treating osteoporosis by decreasing expression of PDE4D or otherwise decreasing levels of active PDE4D in a subject suffering from low BMD. Provided also is a method of targeting pertinent information or administering preventative or therapeutic treatments to a subject based on a subject's PDE4D genotype.

SNP rs869975 is contained within the GPX3 gene, and the TNIP3 gene may not be ruled out due to linkage disequilibrium; therefore the GPX3/TNIP3 region is considered associated with low BMD. The GPX3 gene encodes the Plasma glutathione peroxidase 3 precursor. Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. This enzyme, found mainly in the cytosol of mammalian cells, is unusual in its content of a selenocysteine residue in its active site that is encoded by a TGA opal codon. Selenium deficiency causes bone loss and might contribute to lower BMD. Osteoblasts produce glutathione peroxidase, possibly as a defense against hydrogen peroxide produced by osteoclasts during bone remodeling and thus may contribute to lower BMD. Provided is a method for treating osteoporosis or low bone mineral density by modulating a GPX function in a human suffering from osteoporosis or low bone mineral density.

In the TNIP1/NAF1/ABIN-1 pathway, NAF1 was identified by a yeast two-hybrid screen as an interacting protein to the HIV protein, Nef. Subsequently, it was found to be an A20-binding protein that is critical for the A20-mediated negative feedback regulation of NF-kappa B activation in response to tumor necrosis factor (TNF). As TNF is a critical effector of the pathogenesis of rheumatoid arthritis (RA), Gallagher et. al. (FEBS Lett. 2003 Sep. 11; 551(1-3):8-12), tested TNF-alpha-modulated gene expression in cultured primary human synoviocytes in vitro. Genes upregulated included TNIP1 and implicate TNIP1 as a potential modulator of TNF-alpha bioactivity in RA. The differentiation and functions of osteoclasts are stimulated and regulated by osteoblast/stromal cell derived factors, such as receptor activator of NFKB ligand (RANKL). Provided is a method for treating osteoporosis or low bone mineral density by modulating a TINIP function in a human suffering from osteoporosis or low bone mineral density.

Low BMD and Sample Selection

The present invention is applicable to any disease in which low BMD and/or bone fracture is a factor, and is therefore particularly concerned with diseases such as osteoporosis. Low BMD is defined by the World Health Organization as 2.5 standard deviations below the age-matched mean of bone mineral density for a given population. Bone damage may be defined as any form of structural damage such as fractures or chips of the bone, and degradation or deterioration of the bone other than normal wear and tear resulting from low bone mineral density or another cause. Such low BMD and/or bone damage is associated with osteoporosis.

Osteoporosis, or porous bone, is a disease characterized by low bone mass and structural deterioration of bone tissue, leading to bone fragility and an increased susceptibility to fractures, especially of the hip, spine and wrist. In general, there are two types of osteoporosis: primary and secondary. Approximately 90% of all osteoporosis cases is idiopathic “primary osteoporosis”. Such primary osteoporosis includes postmenopausal osteoporosis, age-associated osteoporosis (affecting a majority of individuals over the age of 70 to 80), and idiopathic osteoporosis affecting middle-aged and younger men and women. “Secondary osteoporosis” is the result of an identifiable disease process or agent.

For some osteoporotic individuals, the loss of bone tissue is sufficiently great so as to cause mechanical failure of the bone structure. Bone fractures often occur, for example, in the hip and spine of women suffering from postmenopausal osteoporosis. Kyphosis (abnormally increased curvature of the thoracic spine) may also result.

The mechanism of bone loss in osteoporotics is believed to involve an imbalance in the process of “bone remodeling”. Bone remodeling occurs throughout life, renewing the skeleton and maintaining the strength of bone. This remodeling involves the erosion and filling of discrete sites on the surface of bones, by an organized group of cells called “basic multicellular units” or “BMUs”. BMUs primarily consist of “osteoclasts”, “osteoblasts”, and their cellular precursors. In the remodeling cycle, bone is resorbed at the site of an “activated” BMU by an osteoclast, forming a resorption cavity. This cavity is then filled with bone by an osteoblast.

Normally, in adults, the remodeling cycle results in a small deficit in bone, due to incomplete filling of the resorption cavity. Thus, even in healthy adults, age-related bone loss occurs. However, in osteoporotics, there is an increase in the number of BMUs that are activated. This increased activation accelerates bone remodeling, resulting in abnormally high bone loss.

Preferred methods for the treatment of osteoporosis include an initial diagnostic step to determine the presence of the disorder. Initial diagnostic steps include determination of bone mass and rate of bone remodeling. The rate of bone remodeling can be determined by the measurement of biochemical markers. See, for example, Hui et al., “The Contribution of Bone Loss to Postmenopausal Osteoporosis” Osteoporosis Int. 30 (1990). Diagnosis of those at risk of developing osteoporosis also allows more effective preventive measures. Part of diagnosis includes specialized tests called bone density tests that measure bone density in various sites of the body. Such methods include the measurement of the radiodensity of skeletal radiographs, quantitative computerized tomography, single energy photon absorptiometry, and dual-energy photon absorptiometry. Diagnostic techniques among those useful herein are described in W. A. Peck et al., Physician's Resource Manual on Osteoporosis (1987), published by the National Osteoporosis Foundation. A bone density test can detect the presence of low BMD before a fracture occurs, predict your chances of fracturing in the future, determine rate of bone loss, and monitor response to treatment.

Based in part upon selection criteria set forth above, individuals having low BMD can be selected for genetic studies. Also, individuals having a family history of low BMD or diagnosed with osteoporosis often are selected for genetic studies. Other selection criteria can include: a tissue or fluid sample derived from an individual characterized as Caucasian; sample derived from an individual of Caucasian paternal and maternal descent, case samples derived from individuals diagnosed with osteoporosis; control samples derived from individuals with normal or high BMD levels and no family history of osteoporosis; and sufficient genomic DNA for all allelotyping and genotyping reactions performed during the study. Phenotype information may included pre- or post-menopausal, familial predisposition, country or origin of mother and father, diagnosis with osteoporosis (date of primary diagnosis, age of individual as of primary diagnosis, osteoporosis-related fracture), biochemical measurements of markers of bone resorption (bone-specific alkaline phosphatase, urinary C-telopeptide of type I collagen, serum osteocalcin), current medication status (thyroid medication, hormone replacement therapy, steroid usage, bisphosphonates and cytotoxic agents for rheumatic diseases). Samples that meet the inclusion criteria and do not meet the exclusion criteria may be added to appropriate pools based on gender and disease status.

Polymorphic Variants Associated with Low BMD

A genetic analysis provided herein linked low BMD with polymorphic variant nucleic acid sequences in the human genome. As used herein, the term “polymorphic site” refers to a region in a nucleic acid at which two or more alternative nucleotide sequences are observed in a significant number of nucleic acid samples from a population of individuals. A polymorphic site may be a nucleotide sequence of two or more nucleotides, an inserted nucleotide or nucleotide sequence, a deleted nucleotide or nucleotide sequence, or a microsatellite, for example. A polymorphic site that is two or more nucleotides in length may be 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 or more, 20 or more, 30 or more, 50 or more, 75 or more, 100 or more, 500 or more, or about 1000 nucleotides in length, where all or some of the nucleotide sequences differ within the region. A polymorphic site is often one nucleotide in length, which is referred to herein as a “single nucleotide polymorphism” or a “SNP.”

Where there are two, three, or four alternative nucleotide sequences at a polymorphic site, each nucleotide sequence is referred to as a “polymorphic variant” or “nucleic acid variant.” Where two polymorphic variants exist, for example, the polymorphic variant represented in a minority of samples from a population is sometimes referred to as a “minor allele” and the polymorphic variant that is more prevalently represented is sometimes referred to as a “major allele.” Many organisms possess a copy of each chromosome (e.g., humans), and those individuals who possess two major alleles or two minor alleles are often referred to as being “homozygous” with respect to the polymorphism, and those individuals who possess one major allele and one minor allele are normally referred to as being “heterozygous” with respect to the polymorphism. Individuals who are homozygous with respect to one allele are sometimes predisposed to a different phenotype as compared to individuals who are heterozygous or homozygous with respect to another allele.

In genetic analysis that associate polymorphic variants with low BMD, samples from individuals having low BMD and individuals not having low BMD often are allelotyped and/or genotyped. The term “allelotype” as used herein refers to a process for determining the allele frequency for a polymorphic variant in pooled DNA samples from cases and controls. By pooling DNA from each group, an allele frequency for each SNP in each group is calculated. These allele frequencies are then compared to one another. The term “genotyped” as used herein refers to a process for determining a genotype of one or more individuals, where a “genotype” is a representation of one or more polymorphic variants in a population.

A genotype or polymorphic variant may be expressed in terms of a “haplotype,” which as used herein refers to two or more polymorphic variants occurring within genomic DNA in a group of individuals within a population. For example, two SNPs may exist within a gene where each SNP position includes a cytosine variation and an adenine variation. Certain individuals in a population may carry one allele (heterozygous) or two alleles (homozygous) having the gene with a cytosine at each SNP position. As the two cytosines corresponding to each SNP in the gene travel together on one or both alleles in these individuals, the individuals can be characterized as having a cytosine/cytosine haplotype with respect to the two SNPs in the gene.

As used herein, the term “phenotype” refers to a trait which can be compared between individuals, such as presence or absence of a condition, a visually observable difference in appearance between individuals, metabolic variations, physiological variations, variations in the function of biological molecules, and the like. An example of a phenotype is occurrence of low BMD or clinically diagnosed osteoporosis.

Researchers sometimes report a polymorphic variant in a database without determining whether the variant is represented in a significant fraction of a population. Because a subset of these reported polymorphic variants are not represented in a statistically significant portion of the population, some of them are sequencing errors and/or not biologically relevant. Thus, it is often not known whether a reported polymorphic variant is statistically significant or biologically relevant until the presence of the variant is detected in a population of individuals and the frequency of the variant is determined. Methods for detecting a polymorphic variant in a population are described herein, specifically in Example 2. A polymorphic variant is statistically significant and often biologically relevant if it is represented in 5% or more of a population, sometimes 10% or more, 15% or more, or 20% or more of a population, and often 25% or more, 30% or more, 35% or more, 40% or more, 45% or more, or 50% or more of a population.

A polymorphic variant may be detected on either or both strands of a double-stranded nucleic acid. Also, a polymorphic variant may be located within an intron or exon of a gene or within a portion of a regulatory region such as a promoter, a 5′ untranslated region (UTR), a 3′ UTR, and in DNA (e.g., genomic DNA (gDNA) and complementary DNA (cDNA)), RNA (e.g., mRNA, tRNA, and rRNA), or a polypeptide. Polymorphic variations may or may not result in detectable differences in gene expression, polypeptide structure, or polypeptide function.

It was determined that polymorphic variations associated with an increased risk of low BMD existed in CETP, PROL4, GRID2, PDE4D and GPX3 nucleotide sequences. In the CETP locus, polymorphic variants at positions selected from the group consisting of rs7500979, rs2217332, rs8044804, rs2270835, rs2133783, rs247609, rs952440, rs881598, rs2291955, rs2518054, rs866038, rs1436425, rs173537, rs247611, rs166017, rs173538, rs193694, rs7205692, rs8048746, rs247618, rs183130, rs6499863, rs4783961, rs3816117, rs711752, rs708272, rs1864163, rs4369653, rs1864165, rs891141, rs891143, rs7205804, rs5885, rs1532625, rs1532624, rs289712, rs7499892, rs5883, rs289714, rs158480, rs289717, rs4344729, rs289718, rs289719, rs2033254, rs4784744, rs291044, rs8053613, rs5881, rs5880, rs7198026, rs5882, rs8045701, rs289741, rs1801706, rs289742, rs289743, rs289746, rs172337, rs289747, rs1566439, rs7205459, rs289749, rs289751, rs8059220, rs8058353, rs289735, rs289737, rs291042, rs1875236, rs821466, rs821465, rs4275846, rs289707, rs821463, rs289706, rs1167741, rs2052880, rs1167742, rs1183256, rs1651665, rs1651666, rs4784751, rs1651667, rs8052091, rs1684574, rs1684575, rs1672865, rs821470, rs1549669, rs291040 and rs289754 were tested for association with low BMD. Polymorphic variants at the following positions were associated with low BMD: rs166017, rs193694, rs7205804, rs1801706, rs7205459 and rs821465. At these positions in SEQ ID NO:1, a thymine at position 14328, a thymine at position 14996, a guanine at position 37336, a guanine at position 50109, a thymine at position 57618, and a guanine at position 68805 were associated with low BMD.

In the PROL4 locus, polymorphic variants at positions selected from the group consisting of rs523051, rs693620, rs2588349, rs2588350, rs619381, rs3759252, rs3759251, rs2418107, rs7303054, rs1838345, rs620878, rs2537817, rs1548803, rs667123, rs1838346, rs2159903, rs3944035, rs3741845, rs2110096, rs759055, rs589377, rs7960194, rs7978242, rs601051, rs4262797, rs2215714, rs1373434, rs2215715, rs612456, rs612808, rs689118, rs597468, rs592864, rs640372, rs7966559, rs654834, rs4763216, rs668521, rs669503, rs3906864, rs3906863, rs7957888, rs9300230, rs7306214, rs763839, rs2418105, rs666841, rs3851578, rs7138797, rs7295252, rs2418106, rs7299578, rs621112, rs3863320, rs1373432, rs1047699, rs1063193, rs2232959, rs2227296, rs1548804, rs2232958, rs2232957, rs2232956, rs1972571, rs3759250, rs3759249, rs1541525, rs2098248, rs2900550, rs7302130, rs4763583, rs4360778, rs1607695, rs1607694, rs2192139, rs7978300, rs7397871, rs4763217, rs2159900, rs10772370, rs7398682, rs2900551, rs2900552, rs2418214, rs2418215, rs965243, rs1117548, rs1520225, rs1520226, rs1520227, rs971919, rs2159901, rs2159902, rs2110099, rs7314847, rs7296003, rs4281556, rs4763219, rs3851579, rs3851580, rs1049119, rs2298866, rs2298865, rs2298864, rs2298863, rs3180393, rs2070837, rs7956204, rs2418216, rs3741844, rs4262798, rs2418217, rs2418218, rs7137492, rs2110100, rs1013312, rs4579993, rs1013313, rs7397106, rs2215716, rs2192140, rs4763589, rs1468697, rs2070837, rs3180393 and rs2298865 were tested for association with low BMD. Polymorphic variants at the following positions were associated with an increased risk of low BMD: rs2588350, rs619381, rs620878, rs759055, rs4262797, rs612808, rs3906863, rs7957888, rs763839, rs2418105, rs666841, rs3851578, rs7299578, rs621112, rs1047699, rs1548804, rs2232956, rs1520227 and rs2215716. At these positions in SEQ ID NO:2, a cytosine at position 2424, a cytosine at position 3625, a guanine at position 7097, an adenine at position 15688, a guanine at position 22861, a cytosine at position 24138, a cytosine at position 32459, an adenine at position 35151, a guanine at position 36930, an adenine at position 37490, a cytosine at position 38432, an adenine at position 38688, a guanine at position 42665, an adenine at position 43038, a cytosine at position 49075, an adenine at position 50773, an adenine at position 52107, a cytosine at position 75246, and a guanine at position 93715 were associated with risk of low BMD.

In the GRID2 locus, polymorphic variants at positions selected from the group consisting of rs1433661, rs1485009, rs7681947, rs1816432, rs1485018, rs1485017, rs7438397, rs6834311, rs1368717, rs1017391, rs2870701, rs7679839, rs1385404, rs1368716, rs4693316, rs1905707, rs1905708, rs1905709, rs3912442, rs2082553, rs6831638, rs5860329, rs2870702, rs2870703, rs1948016, rs6835836, rs1994253, rs1905710, rs1485019, rs978191, rs1385405, rs7694361, rs1905711, rs1905734, rs1485012, rs1485013, rs4692981, rs7670552, rs7670932, rs7688091, rs7440540, rs2171000, rs2870704, rs7655758, rs7661436, rs7662289, rs7667044, rs7691929, rs5860330, rs901013, rs901012, rs901011, rs1948018, rs2870705, rs1948017, rs1905733, rs1385408, rs1385409, rs1385410, rs1485026, rs1485027, rs2904483, rs1385406, rs1905732, rs2046418, rs2200377, rs1905731, rs1905730, rs975713, rs6820985, rs7670441, rs6810794, rs7676623, rs1154861, rs1032125, rs1485022, rs1485024, rs3913651, rs4693319, rs1872383, rs2200376, rs7668090, rs7692930, rs967096, rs6822249, rs6532405, rs1017897, rs7672674, rs7694568, rs2904484, rs7340830, rs1485033, rs2870706, rs1905729, rs4693320, rs6848749, rs6532406, rs6532407, rs1905728, rs6819866, rs1905727, rs7674069, rs1905724, rs1905723, rs1485020 and rs6814101 were tested for association with low BMD. Polymorphic variants at the following positions were associated with an increased risk of low BMD: rs1433661, rs7679839, rs1368716, rs1905707, rs1905708, rs1994253, rs1485019, rs1905734, rs1485012, rs7670552, rs7691929, rs1948018, rs1948017, rs1485024, rs7694568, rs4693320, rs6848749, rs6532406, rs6532407 and rs6819866. At these positions in SEQ ID NO:3, a thymine at position 206, a guanine at position 8612, an adenine at position 9285, a thymine at position 11866, a guanine at position 11958, a cytosine at position 28773, a thymine at position 29876, a cytosine at position 35588, a guanine at position 37663, a thymine at position 39375, a cytosine at position 43705, an adenine at position 48962, a cytosine at position 49110, an adenine at position 65050, a cytosine at position 78331, a thymine at position 85405, a guanine at position 86441, an adenine at position 86967, a cytosine at position 87121, and an adenine at position 90969 were associated with risk of low BMD.

In the PDE4D locus, polymorphic variants at positions selected from the group consisting of rs6886495, rs6450498, rs1472456, rs4700315, rs4700316, rs7714708, rs7710479, rs2968013, rs2968014, rs2968015, rs1391648, rs2055297, rs2055296, rs3989138, rs4700317, rs2036220, rs7727206, rs7723432, rs1546221, rs4479801, rs4395595, rs4395596, rs4699932, rs2936201, rs7356672, rs2936200, rs1909296, rs7703131, rs7445308, rs3087748, rs4321723, rs2968016, rs5868151, rs1874858, rs1874857, rs7712922, rs4631140, rs4469166, rs1078369, rs1078368, rs2968006, rs2968005, rs2936190, rs2409613, rs4415048, rs2968004, rs2968003, rs2968002, rs2936191, rs1498610, rs6874662, rs3060393, rs7729722, rs7733884, rs7714489, rs7735570, rs2936193, rs2291851, rs2291852, rs1498602, rs1995166, rs1498603, rs1498604, rs1498605, rs1948651, rs4699934, rs4700319, rs2279737, rs7720361, rs7706419, rs1006431, rs1353747, rs1498606, rs1353748, rs1553113, rs2968012, rs2968011, rs1498608, rs2936189, rs1498609, rs2968019, rs6891238, rs2968010, rs2968009, rs2936203, rs1498601, rs1498600, rs1498599, rs2936202, rs7730070, rs6450501, rs6450502, rs6889456, rs6894618, rs7706044, rs7707541, rs7712076, rs6892860, rs6867053, rs7737269, rs6864156, rs950447, rs2936196, rs7719347, rs1391649, rs1391650, rs1391651, rs1353749, rs10682149, rs5868153, rs1363882, rs2409626, rs2968018, rs954740, rs986067, rs6869400, and rs5010782 were tested for association with low BMD. Polymorphic variants at the following positions were associated with an increased risk of low BMD: rs7714708, rs1498602, rs4699934, rs1006431, rs1353747, rs1498608, rs1498609, rs2968010, rs2936202 and rs1391649. At these positions in SEQ ID NO:4, an adenine at position 1599, a cytosine at position 39626, a thymine at position 40356, a thymine at position 43555, a thymine at position 44066, a thymine at position 49652, a cytosine at position 51103, an adenine at position 57173, a guanine at position 63980, and an adenine at position 82591 were associated with risk of low BMD.

In the GPX3/TNIP1 locus, polymorphic variants at positions selected from the group consisting of rs1478398, rs1478397, rs1160114, rs1160113, rs1382323, rs1160112, rs7709870, rs7710643, rs7730467, rs6579829, rs6579830, rs6579831, rs6896232, rs1351131, rs1038074, rs1478396, rs6880512, rs4958858, rs4958431, rs4958432, rs6898463, rs4958859, rs4130064, rs4130065, rs4133119, rs4958860, rs4958861, rs4437356, rs4958868, rs1478400, rs6889375, rs1600159, rs6875892, rs4608909, rs2345000, rs4516840, rs2054440, rs707141, rs707142, rs841236, rs707143, rs70144, rs6869405, rs707145, rs707146, rs707148, rs707150, rs5872184, rs3763015, rs2042235, rs3763013, rs2042236, rs1946234, rs1946235, rs1946236, rs8177402, rs8177403, rs8177404, rs8177405, rs8177406, rs8177407, rs8177408, rs8177409, rs6888961, rs8177410, rs8177411, rs8177412, rs8177413, rs870407, rs870406, rs6873202, rs8177414, rs8177415, rs3805435, rs8177416, rs3792799, rs3792798, rs3828599, rs8177417, rs3792797, rs8177418, rs8177419, rs8177420, rs8177421, rs4958872, rs3792796, rs8177422, rs8177423, rs4958434, rs8177424, rs8177425, rs8177426, rs8177427, rs8177429, rs6889737, rs3792795, rs8177430, rs8177431, rs4958873, rs8177432, rs8177433, rs8177434, rs8177435, rs3763011, rs8177436, rs8177437, rs4958874, rs8177439, rs8177440, rs8177441, rs8177442, rs8177443, rs869975, rs869976, rs8177444, rs8177445, rs7721469, rs8177446, rs7704191, rs8177447, rs11548, rs2230303, rs7722386, rs8177448, rs8177449, rs2070593, rs8177450, rs8177451, rs8177452, rs8177453, rs8177454, rs3763010, rs8177455, rs8177456, rs736775, rs2277940, rs8177458, rs8177834, rs3924, rs2233312, rs2233311, rs2233310, rs2233309, rs4958875, rs2233308, rs2233307, rs2233306, rs2233305, rs2233304, rs2233303, rs2233302, rs2287719, rs2287720, rs7727034, rs7727250, rs7709800, rs3840312, rs2287721, rs6875293, rs3805434, rs2080982, rs2080983, rs2287722, rs2233301, rs2233300, rs4958876, rs2233299, rs2233298, rs2287723, rs2161359, rs7734456, rs4292439, rs4958878, rs6862024, rs3834819, rs2233297, rs2233296, rs2233295, rs2233294, rs7713028, rs7713223, rs7713567, rs888989, rs2233293, rs3749657, rs2233292, rs2112635, rs871269, rs3792794, rs6579837, rs3805433, rs5872186, rs2233291, rs2233290, rs2233289, rs4958435, rs4958880, rs1422673, rs2042234, rs3805432, rs3805431, rs2233288, rs2233287, rs3815720, rs3792792, rs3792791, rs2303018, rs3792790, rs4958436, rs2233286, rs2233285, rs7732451, rs2233284, rs1422674, rs3792789, rs4562032, rs6865077, rs1559126, rs3792788, rs1559127, rs3792786, rs6880110, rs6861227, rs3805430, rs1862364, rs4958881, rs3792785, rs6869605, rs6870205, rs4246047, rs4958882, rs3792784, rs3792783 and rs5872188 were tested for association with low BMD. Polymorphic variants at the following positions were associated with an increased risk of low BMD: rs1478398, rs1160114, rs1160113, rs1160112, rs4958858, rs4958431, rs6898463, rs4958859, rs4958860, rs4608909, rs707144, rs2042235, rs3763013, rs2042236, rs8177404, rs8177426, rs8177427, rs8177429, rs3792795, rs4958873, rs8177437, rs869975, rs8177447, rs11548, rs2277940, rs8177834, rs2233311, rs2233302, rs7727034, rs7727250, rs3805434, rs7734456, rs7713028, rs7713223, rs888989, rs3792794, rs4958880, rs1422673, rs3805432 and rs4958436. At these positions in SEQ ID NO:5, an adenine at position 231, a cytosine at position 582, a guanine at position 589, an adenine at position 1066, a thymine at position 5621, a guanine at position 5735, a cytosine at position 6658, a cytosine at position 7901, a thymine at position 15803, a cytosine at position 25599, a thymine at position 31203, a thymine at position 41624, a guanine at position 41671, an adenine at position 41825, a cytosine at position 43294, an adenine at position 46650, an adenine at position 46721, a guanine at position 46808, a guanine at position 47512, an adenine at position 47806, a guanine at position 49097, a guanine at position 50082, a thymine at position 51166, a cytosine at position 51493, a thymine at position 53187, an adenine at position 53699, a thymine at position 53929, a cytosine at position 58808, a cytosine at position 59187, a cytosine at position 59361, a cytosine at position 64049, a guanine at position 70882, an adenine at position 74131, a cytosine at position 74406, a cytosine at position 74740, a guanine at position 78432, a cytosine at position 82187, a cytosine at position 82698, an adenine at position 83214 and a thymine at position 86539 were associated with risk of low BMD.

Based in part upon analyses summarized in FIGS. 1-5, regions with significant association have been identified in loci associated with low BMD. Any polymorphic variants associated with low BMD in a region of significant association can be utilized for embodiments described herein. The following table reports such regions, where “begin” and “end” designate the boundaries of the region according to chromosome positions within the genomic sequence provided in SEQ ID Nos:1-5. The locus, the chromosome on which the locus resides and an incident polymorphism in the locus also are noted.

TABLE 1
COMBINED ASSOCIATION RANGES
Incident SNP	Locus	Chromosome	Begin	End	Size
rs1801706	CETP	16q21	14328	68805	54477
rs1047699	PROL4	12p13	2424	93715	91291
rs1948017	GRID2	4q22	206	90969	90763
rs1498608	PDE4D	5q12	1599	82591	80992
rs869975	GPX3/TNIP1	5q23	231	86539	86308

Additional Polymorphic Variants Associated with Low BMD

Also provided is a method for identifying polymorphic variants proximal to an incident, founder polymorphic variant associated with low BMD. Thus, featured herein are methods for identifying a polymorphic variation associated with low BMD that is proximal to an incident polymorphic variation associated with low BMD, which comprises identifying a polymorphic variant proximal to the incident polymorphic variant associated with low BMD, where the incident polymorphic variant is in a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence. The nucleotide sequence often comprises a polynucleotide sequence selected from the group consisting of (a) a polynucleotide sequence of SEQ ID NO's:1-5; (b) a polynucleotide sequence that encodes a polypeptide having an amino acid sequence encoded by a polynucleotide sequence of SEQ ID NO's:1-5; and (c) a polynucleotide sequence that encodes a polypeptide having an amino acid sequence that is 90% or more identical to an amino acid sequence encoded by a nucleotide sequence of SEQ ID NO's:1-5 or a polynucleotide sequence 90% or more identical to the polynucleotide sequence of SEQ ID NO's:1-5. The presence or absence of an association of the proximal polymorphic variant with low BMD then is determined using a known association method, such as a method described in the Examples hereafter. In an embodiment, the incident polymorphic variant is a polymorphic variant associated with low BMD described herein. In another embodiment, the proximal polymorphic variant identified sometimes is a publicly disclosed polymorphic variant, which for example, sometimes is published in a publicly available database. In other embodiments, the polymorphic variant identified is not publicly disclosed and is discovered using a known method, including, but not limited to, sequencing a region surrounding the incident polymorphic variant in a group of nucleic samples. Thus, multiple polymorphic variants proximal to an incident polymorphic variant are associated with low BMD using this method.

The proximal polymorphic variant often is identified in a region surrounding the incident polymorphic variant. In certain embodiments, this surrounding region is about 50 kb flanking the first polymorphic variant (e.g. about 50 kb 5′ of the first polymorphic variant and about 50 kb 3′ of the first polymorphic variant), and the region sometimes is composed of shorter flanking sequences, such as flanking sequences of about 40 kb, about 30 kb, about 25 kb, about 20 kb, about 15 kb, about 10 kb, about 7 kb, about 5 kb, or about 2 kb 5′ and 3′ of the incident polymorphic variant. In other embodiments, the region is composed of longer flanking sequences, such as flanking sequences of about 55 kb, about 60 kb, about 65 kb, about 70 kb, about 75 kb, about 80 kb, about 85 kb, about 90 kb, about 95 kb, or about 100 kb 5′ and 3′ of the incident polymorphic variant.

In certain embodiments, polymorphic variants associated with low BMD are identified iteratively. For example, a first proximal polymorphic variant is associated with low BMD using the methods described above and then another polymorphic variant proximal to the first proximal polymorphic variant is identified (e.g., publicly disclosed or discovered) and the presence or absence of an association of one or more other polymorphic variants proximal to the first proximal polymorphic variant with low BMD is determined.

The methods described herein are useful for identifying or discovering additional polymorphic variants that may be used to further characterize a gene, region or loci associated with a condition, a disease (e.g., osteoporosis), or a disorder. For example, allelotyping or genotyping data from the additional polymorphic variants may be used to identify a functional mutation or a region of linkage disequilibrium. In certain embodiments, polymorphic variants identified or discovered within a region comprising the first polymorphic variant associated with low BMD are genotyped using the genetic methods and sample selection techniques described herein, and it can be determined whether those polymorphic variants are in linkage disequilibrium with the first polymorphic variant. The size of the region in linkage disequilibrium with the first polymorphic variant also can be assessed using these genotyping methods. Thus, provided herein are methods for determining whether a polymorphic variant is in linkage disequilibrium with a first polymorphic variant associated with low BMD, and such information can be used in prognosis/diagnosis methods described herein.

Isolated Nucleic Acids

Featured herein are isolated CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleic acid variants depicted in SEQ ID NO's:1-10, and substantially identical nucleic acids thereof. A nucleic acid variant may be represented on one or both strands in a double-stranded nucleic acid or on one chromosomal complement (heterozygous) or both chromosomal complements (homozygous)).

As used herein, the term “nucleic acid” includes DNA molecules (e.g., a complementary DNA (cDNA) and genomic DNA (gDNA)) and RNA molecules (e.g., mRNA, rRNA, siRNA and tRNA) and analogs of DNA or RNA, for example, by use of nucleotide analogs. The nucleic acid molecule can be single-stranded and it is often double-stranded. The term “isolated or purified nucleic acid” refers to nucleic acids that are separated from other nucleic acids present in the natural source of the nucleic acid. For example, with regard to genomic DNA, the term “isolated” includes nucleic acids which are separated from the chromosome with which the genomic DNA is naturally associated. An “isolated” nucleic acid is often free of sequences which naturally flank the nucleic acid (i.e., sequences located at the 5′ and/or 3′ ends of the nucleic acid) in the genomic DNA of the organism from which the nucleic acid is derived. For example, in various embodiments, the isolated nucleic acid molecule can contain less than about 5 kb, 4 kb, 3 kb, 2 kb, 1 kb, 0.5 kb or 0.1 kb of 5′ and/or 3′ nucleotide sequences which flank the nucleic acid molecule in genomic DNA of the cell from which the nucleic acid is derived. Moreover, an “isolated” nucleic acid molecule, such as a cDNA molecule, can be substantially free of other cellular material, or culture medium when produced by recombinant techniques, or substantially free of chemical precursors or other chemicals when chemically synthesized. As used herein, the term “gene” refers to a nucleotide sequence that encodes a polypeptide.

The nucleic acid often comprises a part of or all of a nucleotide sequence in SEQ ID NO's:1-5, or a substantially identical sequence thereof. Such a nucleotide sequence sometimes is a 5′ and/or 3′ sequence flanking a polymorphic variant described above that is 5-1000 nucleotides in length, or in some embodiments 5-500, 5-100, 5-75, 5-50, 5-45, 5-40, 5-35, 5-30, 5-25 or 5-20 nucleotides in length. Other embodiments are directed to methods of identifying a polymorphic variation at one or more positions in a nucleic acid (e.g., genotyping at one or more positions in the nucleic acid), such as at a position corresponding to rs1801706 in the CETP gene, rs1047699 in the PROL4 gene, rs1948017 in the GRID2 gene, rs1498608 in the PDE4D gene, or rs869975 in the GPX3 gene.

Also included herein are nucleic acid fragments. These fragments often are a nucleotide sequence identical to a nucleotide sequence of SEQ ID NO's:1-10, a nucleotide sequence substantially identical to a nucleotide sequence of SEQ ID NO's:1-10, or a nucleotide sequence that is complementary to the foregoing. The nucleic acid fragment may be identical, substantially identical or homologous to a nucleotide sequence in an exon or an intron in a nucleotide sequence of SEQ ID NO's:1-5, and may encode a domain or part of a domain of a polypeptide. Sometimes, the fragment will comprises one or more of the polymorphic variations described herein as being associated with low BMD. The nucleic acid fragment is often 50, 100, or 200 or fewer base pairs in length, and is sometimes about 300, 400, 500, 600, 700, 800, 900, 1000, 1100, 1200, 1300, 1400, 1500, 2000, 3000, 4000, 5000, 10000, 15000, or 20000 base pairs in length. A nucleic acid fragment that is complementary to a nucleotide sequence identical or substantially identical to a nucleotide sequence in SEQ ID NO's:1-5 and hybridizes to such a nucleotide sequence under stringent conditions is often referred to as a “probe.” Nucleic acid fragments often include one or more polymorphic sites, or sometimes have an end that is adjacent to a polymorphic site as described hereafter. CETP nucleic acid fragments sometimes encode the mature protein from positions 182 to 1609 of the mRNA sequence (SEQ ID NO: 6), for example.

An example of a nucleic acid fragment is an oligonucleotide. As used herein, the term “oligonucleotide” refers to a nucleic acid comprising about 8 to about 50 covalently linked nucleotides, often comprising from about 8 to about 35 nucleotides, and more often from about 10 to about 25 nucleotides. The backbone and nucleotides within an oligonucleotide may be the same as those of naturally occurring nucleic acids, or analogs or derivatives of naturally occurring nucleic acids, provided that oligonucleotides having such analogs or derivatives retain the ability to hybridize specifically to a nucleic acid comprising a targeted polymorphism. Oligonucleotides described herein may be used as hybridization probes or as components of prognostic or diagnostic assays, for example, as described herein.

Oligonucleotides are typically synthesized using standard methods and equipment, such as the ABI™3900 High Throughput DNA Synthesizer and the EXPEDITE™ 8909 Nucleic Acid Synthesizer, both of which are available from Applied Biosystems (Foster City, Calif.). Analogs and derivatives are exemplified in U.S. Pat. Nos. 4,469,863; 5,536,821; 5,541,306; 5,637,683; 5,637,684; 5,700,922; 5,717,083; 5,719,262; 5,739,308; 5,773,601; 5,886,165; 5,929,226; 5,977,296; 6,140,482; WO 00/56746; WO 01/14398, and related publications. Methods for synthesizing oligonucleotides comprising such analogs or derivatives are disclosed, for example, in the patent publications cited above and in U.S. Pat. Nos. 5,614,622; 5,739,314; 5,955,599; 5,962,674; 6,117,992; in WO 00/75372; and in related publications.

Oligonucleotides may also be linked to a second moiety. The second moiety may be an additional nucleotide sequence such as a tail sequence (e.g., a polyadenosine tail), an adapter sequence (e.g., phage M13 universal tail sequence), and others. Alternatively, the second moiety may be a non-nucleotide moiety such as a moiety which facilitates linkage to a solid support or a label to facilitate detection of the oligonucleotide. Such labels include, without limitation, a radioactive label, a fluorescent label, a chemiluminescent label, a paramagnetic label, and the like. The second moiety may be attached to any position of the oligonucleotide, provided the oligonucleotide can hybridize to the nucleic acid comprising the polymorphism.

Uses of Nucleic Acid Sequence

Nucleic acid coding sequences (e.g., SEQ ID NO: 7-12) may be used for diagnostic purposes for detection and control of polypeptide expression. Also, included herein are oligonucleotide sequences such as antisense RNA, small-interfering RNA (siRNA) and DNA molecules and ribozymes that function to inhibit translation of a polypeptide. Antisense techniques and RNA interference techniques are known in the art and are described herein.

Ribozymes are enzymatic RNA molecules capable of catalyzing the specific cleavage of RNA. The mechanism of ribozyme action involves sequence specific hybridization of the ribozyme molecule to complementary target RNA, followed by endonucleolytic cleavage. For example, hammerhead motif ribozyme molecules may be engineered that specifically and efficiently catalyze endonucleolytic cleavage of RNA sequences corresponding to or complementary to CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequences. Specific ribozyme cleavage sites within any potential RNA target are initially identified by scanning the target molecule for ribozyme cleavage sites which include the following sequences, GUA, GUU and GUC. Once identified, short RNA sequences of between fifteen (15) and twenty (20) ribonucleotides corresponding to the region of the target gene containing the cleavage site may be evaluated for predicted structural features such as secondary structure that may render the oligonucleotide sequence unsuitable. The suitability of candidate targets may also be evaluated by testing their accessibility to hybridization with complementary oligonucleotides, using ribonuclease protection assays.

Antisense RNA and DNA molecules, siRNA and ribozymes may be prepared by any method known in the art for the synthesis of RNA molecules. These include techniques for chemically synthesizing oligodeoxyribonucleotides well known in the art such as solid phase phosphoramidite chemical synthesis. Alternatively, RNA molecules may be generated by in vitro and in vivo transcription of DNA sequences encoding the antisense RNA molecule. Such DNA sequences may be incorporated into a wide variety of vectors which incorporate suitable RNA polymerase promoters such as the T7 or SP6 polymerase promoters. Alternatively, antisense cDNA constructs that synthesize antisense RNA constitutively or inducibly, depending on the promoter used, can be introduced stably into cell lines.

DNA encoding a polypeptide also may have a number of uses for the diagnosis of diseases, including low BMD, resulting from aberrant expression of a target gene described herein. For example, the nucleic acid sequence may be used in hybridization assays of biopsies or autopsies to diagnose abnormalities of expression or function (e.g., Southern or Northern blot analysis, in situ hybridization assays).

In addition, the expression of a polypeptide during embryonic development may also be determined using nucleic acid encoding the polypeptide. As addressed, infra, production of functionally impaired polypeptide is the cause of various disease states, such as osteoporosis. In situ hybridizations using polypeptide as a probe may be employed to predict problems related to low BMD. Further, as indicated, infra, administration of human active polypeptide, recombinantly produced as described herein, may be used to treat disease states related to functionally impaired polypeptide. Alternatively, gene therapy approaches may be employed to remedy deficiencies of functional polypeptide or to replace or compete with dysfunctional polypeptide.

Expression Vectors, Host Cells, and Genetically Engineered Cells

Provided herein are nucleic acid vectors, often expression vectors, which contain a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence or a substantially identical sequence thereof. As used herein, the term “vector” refers to a nucleic acid molecule capable of transporting another nucleic acid to which it has been linked and can include a plasmid, cosmid, or viral vector. The vector can be capable of autonomous replication or it can integrate into a host DNA. Viral vectors may include replication defective retroviruses, adenoviruses and adeno-associated viruses for example.

A vector can include a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence in a form suitable for expression of an encoded target polypeptide or target nucleic acid in a host cell. A “target polypeptide” is a polypeptide encoded by a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence or a substantially identical nucleotide sequence thereof. The recombinant expression vector typically includes one or more regulatory sequences operatively linked to the nucleic acid sequence to be expressed. The term “regulatory sequence” includes promoters, enhancers and other expression control elements (e.g., polyadenylation signals). Regulatory sequences include those that direct constitutive expression of a nucleotide sequence, as well as tissue-specific regulatory and/or inducible sequences. The design of the expression vector can depend on such factors as the choice of the host cell to be transformed, the level of expression of polypeptide desired, and the like. Expression vectors can be introduced into host cells to produce target polypeptides, including fusion polypeptides.

Recombinant expression vectors can be designed for expression of target polypeptides in prokaryotic or eukaryotic cells. For example, target polypeptides can be expressed in E. coli, insect cells (e.g., using baculovirus expression vectors), yeast cells, or mammalian cells. Suitable host cells are discussed further in Goeddel, Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, Calif. (1990). Alternatively, the recombinant expression vector can be transcribed and translated in vitro, for example using T7 promoter regulatory sequences and T7 polymerase.

Expression of polypeptides in prokaryotes is most often carried out in E. coli with vectors containing constitutive or inducible promoters directing the expression of either fusion or non-fusion polypeptides. Fusion vectors add a number of amino acids to a polypeptide encoded therein, usually to the amino terminus of the recombinant polypeptide. Such fusion vectors typically serve three purposes: 1) to increase expression of recombinant polypeptide; 2) to increase the solubility of the recombinant polypeptide; and 3) to aid in the purification of the recombinant polypeptide by acting as a ligand in affinity purification. Often, a proteolytic cleavage site is introduced at the junction of the fusion moiety and the recombinant polypeptide to enable separation of the recombinant polypeptide from the fusion moiety subsequent to purification of the fusion polypeptide. Such enzymes, and their cognate recognition sequences, include Factor Xa, thrombin and enterokinase. Typical fusion expression vectors include pGEX (Pharmacia Biotech Inc; Smith & Johnson, Gene 67: 31-40 (1988)), pMAL (New England Biolabs, Beverly, Mass.) and pRIT5 (Pharmacia, Piscataway, N.J.) which fuse glutathione S-transferase (GST), maltose E binding polypeptide, or polypeptide A, respectively, to the target recombinant polypeptide.

Purified fusion polypeptides can be used in screening assays and to generate antibodies specific for target polypeptides. In a therapeutic embodiment, fusion polypeptide expressed in a retroviral expression vector is used to infect bone marrow cells that are subsequently transplanted into irradiated recipients. The pathology of the subject recipient is then examined after sufficient time has passed (e.g., six (6) weeks).

Expressing the polypeptide in host bacteria with an impaired capacity to proteolytically cleave the recombinant polypeptide is often used to maximize recombinant polypeptide expression (Gottesman, S., Gene Expression Technology: Methods in Enzymology, Academic Press, San Diego, Calif. 185: 119-128 (1990)). Another strategy is to alter the nucleotide sequence of the nucleic acid to be inserted into an expression vector so that the individual codons for each amino acid are those preferentially utilized in E. coli (Wada et al., Nucleic Acids Res. 20: 2111-2118 (1992)). Such alteration of nucleotide sequences can be carried out by standard DNA synthesis techniques.

When used in mammalian cells, the expression vector's control functions are often provided by viral regulatory elements. For example, commonly used promoters are derived from polyoma, Adenovirus 2, cytomegalovirus and Simian Virus 40. Recombinant mammalian expression vectors are often capable of directing expression of the nucleic acid in a particular cell type (e.g., tissue-specific regulatory elements are used to express the nucleic acid). Non-limiting examples of suitable tissue-specific promoters include an albumin promoter (liver-specific; Pinkert et al., Genes Dev. 1: 268-277 (1987)), lymphoid-specific promoters (Calame & Eaton, Adv. Immunol. 43: 235-275 (1988)), promoters of T cell receptors (Winoto & Baltimore, EMBO J. 8: 729-733 (1989)) promoters of immunoglobulins (Banerji et al., Cell 33: 729-740 (1983); Queen & Baltimore, Cell 33: 741-948 (1983)), neuron-specific promoters (e.g., the neurofilament promoter; Byrne & Ruddle, Proc. Natl. Acad. Sci. USA 86: 5473-5477 (1989)), pancreas-specific promoters (Edlund et al., Science 230: 912-916 (1985)), and mammary gland-specific promoters (e.g., milk whey promoter; U.S. Pat. No. 4,873,316 and European Application Publication No. 264,166). Developmentally-regulated promoters are sometimes utilized, for example, the murine hox promoters (Kessel & Gruss, Science 249: 374-379 (1990)) and the α-fetopolypeptide promoter (Campes & Tilghman, Genes Dev. 3: 537-546 (1989)).

A CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleic acid may also be cloned into an expression vector in an antisense orientation. Regulatory sequences (e.g., viral promoters and/or enhancers) operatively linked to a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleic acid cloned in the antisense orientation can be chosen for directing constitutive, tissue specific or cell type specific expression of antisense RNA in a variety of cell types. Antisense expression vectors can be in the form of a recombinant plasmid, phagemid or attenuated virus. For a discussion of the regulation of gene expression using antisense genes see, e.g., Weintraub et al., Antisense RNA as a molecular tool for genetic analysis, Reviews—Trends in Genetics, Vol. 1(1) (1986).

Also provided herein are host cells that include a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence within a recombinant expression vector or a fragment of such a nucleotide sequence which facilitate homologous recombination into a specific site of the host cell genome. The terms “host cell” and “recombinant host cell” are used interchangeably herein. Such terms refer not only to the particular subject cell but rather also to the progeny or potential progeny of such a cell. Because certain modifications may occur in succeeding generations due to either mutation or environmental influences, such progeny may not, in fact, be identical to the parent cell, but are still included within the scope of the term as used herein. A host cell can be any prokaryotic or eukaryotic cell. For example, a target polypeptide can be expressed in bacterial cells such as E. coli, insect cells, yeast or mammalian cells (such as Chinese hamster ovary cells (CHO) or COS cells). Other suitable host cells are known to those skilled in the art.

Vectors can be introduced into host cells via conventional transformation or transfection techniques. As used herein, the terms “transformation” and “transfection” are intended to refer to a variety of art-recognized techniques for introducing foreign nucleic acid (e.g., DNA) into a host cell, including calcium phosphate or calcium chloride co-precipitation, transduction/infection, DEAE-dextran-mediated transfection, lipofection, or electroporation.

A host cell provided herein can be used to produce (i.e., express) a target polypeptide or a substantially identical polypeptide thereof. Accordingly, further provided are methods for producing a target polypeptide using host cells described herein. In one embodiment, the method includes culturing host cells into which a recombinant expression vector encoding a target polypeptide has been introduced in a suitable medium such that a target polypeptide is produced. In another embodiment, the method further includes isolating a target polypeptide from the medium or the host cell.

Also provided are cells or purified preparations of cells which include a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 transgene, or which otherwise misexpress target polypeptide. Cell preparations can consist of human or non-human cells, e.g., rodent cells, e.g., mouse or rat cells, rabbit cells, or pig cells. In preferred embodiments, the cell or cells include a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 transgene (e.g., a heterologous form of a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 gene, such as a human gene expressed in non-human cells). The transgene can be misexpressed, e.g., overexpressed or underexpressed. In other preferred embodiments, the cell or cells include a gene which misexpress an endogenous target polypeptide (e.g., expression of a gene is disrupted, also known as a knockout). Such cells can serve as a model for studying disorders which are related to mutated or mis-expressed alleles or for use in drug screening. Also provided are human cells (e.g., a hematopoietic stem cells) transformed with a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleic acid.

Also provided are cells or a purified preparation thereof (e.g., human cells) in which an endogenous CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleic acid is under the control of a regulatory sequence that does not normally control the expression of the endogenous gene corresponding to a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence. The expression characteristics of an endogenous gene within a cell (e.g., a cell line or microorganism) can be modified by inserting a heterologous DNA regulatory element into the genome of the cell such that the inserted regulatory element is operably linked to the corresponding endogenous gene. For example, an endogenous corresponding gene (e.g., a gene which is “transcriptionally silent,” not normally expressed, or expressed only at very low levels) may be activated by inserting a regulatory element which is capable of promoting the expression of a normally expressed gene product in that cell. Techniques such as targeted homologous recombinations, can be used to insert the heterologous DNA as described in, e.g., Chappel, U.S. Pat. No. 5,272,071; WO 91/06667, published on May 16, 1991.

Transyenic Animals

Non-human transgenic animals that express a heterologous target polypeptide (e.g., expressed from a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleic acid or substantially identical sequence thereof) can be generated. Such animals are useful for studying the function and/or activity of a target polypeptide and for identifying and/or evaluating modulators of the activity of CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleic acids and encoded polypeptides. As used herein, a “transgenic animal” is a non-human animal such as a mammal (e.g., a non-human primate such as chimpanzee, baboon, or macaque; an ungulate such as an equine, bovine, or caprine; or a rodent such as a rat, a mouse, or an Israeli sand rat), a bird (e.g., a chicken or a turkey), an amphibian (e.g., a frog, salamander, or newt), or an insect (e.g., Drosophila melanogaster), in which one or more of the cells of the animal includes a transgene. A transgene is exogenous DNA or a rearrangement (e.g., a deletion of endogenous chromosomal DNA) that is often integrated into or occurs in the genome of cells in a transgenic animal. A transgene can direct expression of an encoded gene product in one or more cell types or tissues of the transgenic animal, and other transgenes can reduce expression (e.g., a knockout). Thus, a transgenic animal can be one in which an endogenous nucleic acid homologous to a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleic acid has been altered by homologous recombination between the endogenous gene and an exogenous DNA molecule introduced into a cell of the animal (e.g., an embryonic cell of the animal) prior to development of the animal.

Intronic sequences and polyadenylation signals can also be included in the transgene to increase expression efficiency of the transgene. One or more tissue-specific regulatory sequences can be operably linked to a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence to direct expression of an encoded polypeptide to particular cells. A transgenic founder animal can be identified based upon the presence of a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence in its genome and/or expression of encoded mRNA in tissues or cells of the animals. A transgenic founder animal can then be used to breed additional animals carrying the transgene. Moreover, transgenic animals carrying a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence can further be bred to other transgenic animals carrying other transgenes.

Target polypeptides can be expressed in transgenic animals or plants by introducing, for example, a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleic acid into the genome of an animal that encodes the target polypeptide. In preferred embodiments the nucleic acid is placed under the control of a tissue specific promoter, e.g., a milk or egg specific promoter, and recovered from the milk or eggs produced by the animal. Also included is a population of cells from a transgenic animal.

Target Polypeptides

Also featured herein are isolated target polypeptides, which are encoded by a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence (e.g., SEQ ID NO's:1-10) or a substantially identical nucleotide sequence thereof, such as the polypeptides having amino acid sequences in SEQ ID NO's:11-15. The term “polypeptide” as used herein includes proteins and peptides. An “isolated” or “purified” polypeptide or protein is substantially free of cellular material or other contaminating proteins from the cell or tissue source from which the protein is derived, or substantially free from chemical precursors or other chemicals when chemically synthesized. In one embodiment, the language “substantially free” means preparation of a target polypeptide having less than about 30%, 20%, 10% and more preferably 5% (by dry weight), of non-target polypeptide (also referred to herein as a “contaminating protein”), or of chemical precursors or non-target chemicals. When the target polypeptide or a biologically active portion thereof is recombinantly produced, it is also preferably substantially free of culture medium, specifically, where culture medium represents less than about 20%, sometimes less than about 10%, and often less than about 5% of the volume of the polypeptide preparation. Isolated or purified target polypeptide preparations are sometimes 0.01 milligrams or more or 0.1 milligrams or more, and often 1.0 milligrams or more and 10 milligrams or more in dry weight.

Further included herein are target polypeptide fragments. The polypeptide fragment may be a domain or part of a domain of a target polypeptide. In addition, the polypeptide fragment may be a full-length polypeptide or a mature polypeptide (i.e., the polypeptide minus the signal peptide). For example, a fragment sometimes is a CTEP mature protein that corresponds to amino acid positions 18493 of SEQ ID NO:11. The polypeptide fragment may have increased, decreased or unexpected biological activity. The polypeptide fragment is often 50 or fewer, 100 or fewer, or 200 or fewer amino acids in length, and is sometimes 300, 400, 500, 600, 700, or 900 or fewer amino acids in length.

Substantially identical target polypeptides may depart from the amino acid sequences of target polypeptides in different manners. For example, conservative amino acid modifications may be introduced at one or more positions in the amino acid sequences of target polypeptides. A “conservative amino acid substitution” is one in which the amino acid is replaced by another amino acid having a similar structure and/or chemical function. Families of amino acid residues having similar structures and functions are well known. These families include amino acids with basic side chains (e.g., lysine, arginine, histidine), acidic side chains (e.g., aspartic acid, glutamic acid), uncharged polar side chains (e.g., glycine, asparagine, glutamine, serine, threonine, tyrosine, cysteine), nonpolar side chains (e.g., alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan), beta-branched side chains (e.g., threonine, valine, isoleucine) and aromatic side chains (e.g., tyrosine, phenylalanine, tryptophan, histidine). Also, essential and non-essential amino acids may be replaced. A “non-essential” amino acid is one that can be altered without abolishing or substantially altering the biological function of a target polypeptide, whereas altering an “essential” amino acid abolishes or substantially alters the biological function of a target polypeptide. Amino acids that are conserved among target polypeptides are typically essential amino acids.

Also, target polypeptides may exist as chimeric or fusion polypeptides. As used herein, a target “chimeric polypeptide” or target “fusion polypeptide” includes a target polypeptide linked to a non-target polypeptide. A “non-target polypeptide” refers to a polypeptide having an amino acid sequence corresponding to a polypeptide which is not substantially identical to the target polypeptide, which includes, for example, a polypeptide that is different from the target polypeptide and derived from the same or a different organism. The target polypeptide in the fusion polypeptide can correspond to an entire or nearly entire target polypeptide or a fragment thereof. The non-target polypeptide can be fused to the N-terminus or C-terminus of the target polypeptide.

Fusion polypeptides can include a moiety having high affinity for a ligand. For example, the fusion polypeptide can be a GST-target fusion polypeptide in which the target sequences are fused to the C-terminus of the GST sequences, or a polyhistidine-target fusion polypeptide in which the target polypeptide is fused at the N- or C-terminus to a string of histidine residues. Such fusion polypeptides can facilitate purification of recombinant target polypeptide. Expression vectors are commercially available that already encode a fusion moiety (e.g., a GST polypeptide), and a nucleotide sequence in SEQ ID NO's:1-10, or a substantially identical nucleotide sequence thereof, can be cloned into an expression vector such that the fusion moiety is linked in-frame to the target polypeptide. Further, the fusion polypeptide can be a target polypeptide containing a heterologous signal sequence at its N-terminus. In certain host cells (e.g., mammalian host cells), expression, secretion, cellular internalization, and cellular localization of a target polypeptide can be increased through use of a heterologous signal sequence. Fusion polypeptides can also include all or a part of a serum polypeptide (e.g., an IgG constant region or human serum albumin).

Target polypeptides can be incorporated into pharmaceutical compositions and administered to a subject in vivo. Administration of these target polypeptides can be used to affect the bioavailability of a substrate of the target polypeptide and may effectively increase target polypeptide biological activity in a cell. Target fusion polypeptides may be useful therapeutically for the treatment of disorders caused by, for example, (i) aberrant modification or mutation of a gene encoding a target polypeptide; (ii) mis-regulation of the gene encoding the target polypeptide; and (iii) aberrant post-translational modification of a target polypeptide. Also, target polypeptides can be used as immunogens to produce anti-target antibodies in a subject, to purify target polypeptide ligands or binding partners, and in screening assays to identify molecules which inhibit or enhance the interaction of a target polypeptide with a substrate.

In addition, polypeptides can be chemically synthesized using techniques known in the art (See, e.g., Creighton, 1983 Proteins. New York, N.Y.: W. H. Freeman and Company, and Hunkapiller et al., (1984) Nature July 12-18; 310(5973):105-11). For example, a relative short fragment can be synthesized by use of a peptide synthesizer. Furthermore, if desired, non-classical amino acids or chemical amino acid analogs can be introduced as a substitution or addition into the fragment sequence. Non-classical amino acids include, but are not limited to, to the D-isomers of the common amino acids, 2,4-diaminobutyric acid, a-amino isobutyric acid, 4-aminobutyric acid, Abu, 2-amino butyric acid, g-Abu, e-Ahx, 6-amino hexanoic acid, Aib, 2-amino isobutyric acid, 3-amino propionic acid, ornithine, norleucine, norvaline, hydroxyproline, sarcosine, citrulline, homocitrulline, cysteic acid, t-butylglycine, t-butylalanine, phenylglycine, cyclohexylalanine, b-alanine, fluoroamino acids, designer amino acids such as b-methyl amino acids, Ca-methyl amino acids, Na-methyl amino acids, and amino acid analogs in general. Furthermore, the amino acid can be D (dextrorotary) or L (levorotary).

Polypeptides and polypeptide fragments sometimes are differentially modified during or after translation, e.g., by glycosylation, acetylation, phosphorylation, amidation, derivatization by known protecting/blocking groups, proteolytic cleavage, linkage to an antibody molecule or other cellular ligand, etc. Any of numerous chemical modifications may be carried out by known techniques, including but not limited, to specific chemical cleavage by cyanogen bromide, trypsin, chymotrypsin, papain, V8 protease, NaBH4; acetylation, formylation, oxidation, reduction; metabolic synthesis in the presence of tunicamycin; and the like. Additional post-translational modifications include, for example, N-linked or O-linked carbohydrate chains, processing of N-terminal or C-terminal ends), attachment of chemical moieties to the amino acid backbone, chemical modifications of N-linked or O-linked carbohydrate chains, and addition or deletion of an N-terminal methionine residue as a result of prokaryotic host cell expression. The polypeptide fragments may also be modified with a detectable label, such as an enzymatic, fluorescent, isotopic or affinity label to allow for detection and isolation of the polypeptide.

Also provided are chemically modified derivatives of polypeptides that can provide additional advantages such as increased solubility, stability and circulating time of the polypeptide, or decreased immunogenicity (see e.g., U.S. Pat. No. 4,179,337. The chemical moieties for derivitization may be selected from water soluble polymers such as polyethylene glycol, ethylene glycol/propylene glycol copolymers, carboxymethylcellulose, dextran, polyvinyl alcohol and the like. The polypeptides may be modified at random positions within the molecule, or at predetermined positions within the molecule and may include one, two, three or more attached chemical moieties.

The polymer may be of any molecular weight, and may be branched or unbranched. For polyethylene glycol, the preferred molecular weight is between about 1 kDa and about 100 kDa (the term “about” indicating that in preparations of polyethylene glycol, some molecules will weigh more, some less, than the stated molecular weight) for ease in handling and manufacturing. Other sizes may be used, depending on the desired therapeutic profile (e.g., the duration of sustained release desired, the effects, if any on biological activity, the ease in handling, the degree or lack of antigenicity and other known effects of the polyethylene glycol to a therapeutic protein or analog).

The polymers should be attached to the polypeptide with consideration of effects on functional or antigenic domains of the polypeptide. There are a number of attachment methods available to those skilled in the art (e.g., EP 0 401 384 (coupling PEG to G-CSF) and Malik et al. (1992) Exp Hematol. September; 20(8):1028-35 (pegylation of GM-CSF using tresyl chloride)). For example, polyethylene glycol may be covalently bound through amino acid residues via a reactive group, such as a free amino or carboxyl group. Reactive groups are those to which an activated polyethylene glycol molecule may be bound. The amino acid residues having a free amino group may include lysine residues and the N-terminal amino acid residues; those having a free carboxyl group may include aspartic acid residues, glutamic acid residues and the C-terminal amino acid residue. Sulfhydryl groups may also be used as a reactive group for attaching the polyethylene glycol molecules. For therapeutic purposes, the attachment sometimes is at an amino group, such as attachment at the N-terminus or lysine group.

Proteins can be chemically modified at the N-terminus. Using polyethylene glycol as an illustration of such a composition, one may select from a variety of polyethylene glycol molecules (by molecular weight, branching, and the like), the proportion of polyethylene glycol molecules to protein (polypeptide) molecules in the reaction mix, the type of pegylation reaction to be performed, and the method of obtaining the selected N-terminally pegylated protein. The method of obtaining the N-terminally pegylated preparation (i.e., separating this moiety from other monopegylated moieties if necessary) may be by purification of the N-terminally pegylated material from a population of pegylated protein molecules. Selective proteins chemically modified at the N-terminus may be accomplished by reductive alkylation, which exploits differential reactivity of different types of primary amino groups (lysine versus the N-terminal) available for derivatization in a particular protein. Under the appropriate reaction conditions, substantially selective derivatization of the protein at the N-terminus with a carbonyl group containing polymer is achieved.

Substantially Identical Nucleic Acids and Polypeptides

Nucleotide sequences and polypeptide sequences that are substantially identical to a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence and the target polypeptide sequences encoded by those nucleotide sequences, respectively, are included herein. The term “substantially identical” as used herein refers to two or more nucleic acids or polypeptides sharing one or more identical nucleotide sequences or polypeptide sequences, respectively. Included are nucleotide sequences or polypeptide sequences that are 55% or more, 60% or more, 65% or more, 70% or more, 75% or more, 80% or more, 85% or more, 90% or more, 95% or more (each often within a 1%, 2%, 3% or 4% variability) identical to a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence or the encoded target polypeptide amino acid sequences. One test for determining whether two nucleic acids are substantially identical is to determine the percent of identical nucleotide sequences or polypeptide sequences shared between the nucleic acids or polypeptides.

Calculations of sequence identity are often performed as follows. Sequences are aligned for optimal comparison purposes (e.g., gaps can be introduced in one or both of a first and a second amino acid or nucleic acid sequence for optimal alignment and non-homologous sequences can be disregarded for comparison purposes). The length of a reference sequence aligned for comparison purposes is sometimes 30% or more, 40% or more, 50% or more, often 60% or more, and more often 70% or more, 80% or more, 90% or more, or 100% of the length of the reference sequence. The nucleotides or amino acids at corresponding nucleotide or polypeptide positions, respectively, are then compared among the two sequences. When a position in the first sequence is occupied by the same nucleotide or amino acid as the corresponding position in the second sequence, the nucleotides or amino acids are deemed to be identical at that position. The percent identity between the two sequences is a function of the number of identical positions shared by the sequences, taking into account the number of gaps, and the length of each gap, introduced for optimal alignment of the two sequences.

Comparison of sequences and determination of percent identity between two sequences can be accomplished using a mathematical algorithm. Percent identity between two amino acid or nucleotide sequences can be determined using the algorithm of Meyers & Miller, CABIOS 4: 11-17 (1989), which has been incorporated into the ALIGN program (version 2.0), using a PAM120 weight residue table, a gap length penalty of 12 and a gap penalty of 4. Also, percent identity between two amino acid sequences can be determined using the Needleman & Wunsch, J. Mol. Biol. 48: 444-453 (1970) algorithm which has been incorporated into the GAP program in the GCG software package (available at the http address www.gcg.com), using either a Blossum 62 matrix or a PAM250 matrix, and a gap weight of 16, 14, 12, 10, 8, 6, or 4 and a length weight of 1, 2, 3, 4, 5, or 6. Percent identity between two nucleotide sequences can be determined using the GAP program in the GCG software package (available at http address www.gcg.com), using a NWSgapdna.CMP matrix and a gap weight of 40, 50, 60, 70, or 80 and a length weight of 1, 2, 3, 4, 5, or 6. A set of parameters often used is a Blossum 62 scoring matrix with a gap open penalty of 12, a gap extend penalty of 4, and a frameshift gap penalty of 5.

Another manner for determining if two nucleic acids are substantially identical is to assess whether a polynucleotide homologous to one nucleic acid will hybridize to the other nucleic acid under stringent conditions. As use herein, the term “stringent conditions” refers to conditions for hybridization and washing. Stringent conditions are known to those skilled in the art and can be found in Current Protocols in Molecular Biology, John Wiley & Sons, N.Y., 6.3.1-6.3.6 (1989). Aqueous and non-aqueous methods are described in that reference and either can be used. An example of stringent hybridization conditions is hybridization in 6× sodium chloride/sodium citrate (SSC) at about 45° C., followed by one or more washes in 0.2×SSC, 0.1% SDS at 50° C. Another example of stringent hybridization conditions are hybridization in 6× sodium chloride/sodium citrate (SSC) at about 45° C., followed by one or more washes in 0.2×SSC, 0.1% SDS at 55° C. A further example of stringent hybridization conditions is hybridization in 6× sodium chloride/sodium citrate (SSC) at about 45° C., followed by one or more washes in 0.2×SSC, 0.1% SDS at 60° C. Often, stringent hybridization conditions are hybridization in 6× sodium chloride/sodium citrate (SSC) at about 45° C., followed by one or more washes in 0.2×SSC, 0.1% SDS at 65° C. More often, stringency conditions are 0.5M sodium phosphate, 7% SDS at 65° C., followed by one or more washes at 0.2×SSC, 1% SDS at 65° C.

An example of a substantially identical nucleotide sequence to a nucleotide sequence in SEQ ID NO's:1-10 is one that has a different nucleotide sequence but still encodes the same polypeptide sequence encoded by the nucleotide sequence in SEQ ID NO's:1-10. Another example is a nucleotide sequence that encodes a polypeptide having a polypeptide sequence that is more than 70% or more identical to, sometimes more than 75% or more, 80% or more, or 85% or more identical to, and often more than 90% or more and 95% or more identical to a polypeptide sequence encoded by a nucleotide sequence in SEQ ID NO's:1-10. As used herein, “SEQ ID NO's:1-10” typically refers to one or more sequences in SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, and/or 10. Many of the embodiments described herein are applicable to (a) a nucleotide sequence of SEQ ID NO's:1-10; (b) a nucleotide sequence which encodes a polypeptide consisting of an amino acid sequence encoded by a nucleotide sequence of SEQ ID NO's:1-10; (c) a nucleotide sequence which encodes a polypeptide that is 90% or more identical to an amino acid sequence encoded by a nucleotide sequence of SEQ ID NO's:1-10, or a nucleotide sequence about 90% or more identical to a nucleotide sequence of SEQ ID NO's:1-10; (d) a fragment of a nucleotide sequence of (a), (b), or (c); and/or a nucleotide sequence complementary to the nucleotide sequences of (a), (b), (c) and/or (d), where nucleotide sequences of (b) and (c), fragments of (b) and (c) and nucleotide sequences complementary to (b) and (c) are examples of substantially identical nucleotide sequences. Examples of substantially identical nucleotide sequences include nucleotide sequences from subjects that differ by naturally occurring genetic variance, which sometimes is referred to as background genetic variance (e.g. nucleotide sequences differing by natural genetic variance sometimes are 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to one another).

Nucleotide sequences in SEQ ID NO's:1-10 and amino acid sequences of encoded polypeptides can be used as “query sequences” to perform a search against public databases to identify other family members or related sequences, for example. Such searches can be performed using the NBLAST and XBLAST programs (version 2.0) of Altschul et al., J. Mol. Biol. 215: 403-10 (1990). BLAST nucleotide searches can be performed with the NBLAST program, score=100, wordlength=12 to obtain nucleotide sequences homologous to nucleotide sequences in SEQ ID NO's:1-10. BLAST polypeptide searches can be performed with the XBLAST program, score=50, wordlength=3 to obtain amino acid sequences homologous to polypeptides encoded by the nucleotide sequences of SEQ ID NO's:1-10. To obtain gapped alignments for comparison purposes, Gapped BLAST can be utilized as described in Altschul et al., Nucleic Acids Res. 25(17): 3389-3402 (1997). When utilizing BLAST and Gapped BLAST programs, default parameters of the respective programs (e.g., XBLAST and NBLAST) can be used (see the http address www.ncbi.nlm.nih.gov).

A nucleic acid that is substantially identical to a nucleotide sequence in SEQ ID NO's:1-10 may include polymorphic sites at positions equivalent to those described herein when the sequences are aligned. For example, using the alignment procedures described herein, SNPs in a sequence substantially identical to a sequence in SEQ ID NO's:1-10 can be identified at nucleotide positions that match with or correspond to (i.e., align) nucleotides at SNP positions in each nucleotide sequence in SEQ ID NO's:1-10. Also, where a polymorphic variation results in an insertion or deletion, insertion or deletion of a nucleotide sequence from a reference sequence can change the relative positions of other polymorphic sites in the nucleotide sequence.

Substantially identical nucleotide and polypeptide sequences include those that are naturally occurring, such as allelic variants (same locus), splice variants, homologs (different locus), and orthologs (different organism) or can be non-naturally occurring. Non-naturally occurring variants can be generated by mutagenesis techniques, including those applied to polynucleotides, cells, or organisms. The variants can contain nucleotide substitutions, deletions, inversions and insertions. Variation can occur in either or both the coding and non-coding regions. The variations can produce both conservative and non-conservative amino acid substitutions (as compared in the encoded product). Orthologs, homologs, allelic variants, and splice variants can be identified using methods known in the art. These variants normally comprise a nucleotide sequence encoding a polypeptide that is 50% or more, about 55% or more, often about 70-75% or more or about 80-85% or more, and sometimes about 90-95% or more identical to the amino acid sequences of target polypeptides or a fragment thereof. Such nucleic acid molecules can readily be identified as being able to hybridize under stringent conditions to a nucleotide sequence in SEQ ID NO's:1-10 or a fragment of this sequence. Nucleic acid molecules corresponding to orthologs, homologs, and allelic variants of a nucleotide sequence in SEQ ID NO's:1-10 can further be identified by mapping the sequence to the same chromosome or locus as the nucleotide sequence in SEQ ID NO's:1-10.

Also, substantially identical nucleotide sequences may include codons that are altered with respect to the naturally occurring sequence for enhancing expression of a target polypeptide in a particular expression system. For example, the nucleic acid can be one in which one or more codons are altered, and often 10% or more or 20% or more of the codons are altered for optimized expression in bacteria (e.g., E. coli), yeast (e.g., S. cervesiae), human (e.g., 293 cells), insect, or rodent (e.g., hamster) cells.

Methods for Identifying Subjects at Risk of Osteoporosis and Risk of Osteoporosis in a Subject

Methods for prognosing and diagnosing low BMD and its related disorders (e.g., osteoporosis) are included herein. These methods include detecting the presence or absence of one or more polymorphic variations in a nucleotide sequence associated with low BMD, such as variants in or around the loci set forth herein, or a substantially identical sequence thereof, in a sample from a subject, where the presence of a polymorphic variant described herein is indicative of a risk of low BMD or one or more low BMD related disorders (e.g., osteoporosis). Determining a risk of osteoporosis refers to determining whether an individual is at an increased risk of osteoporosis (e.g., intermediate risk or higher risk).

Thus, featured herein is a method for identifying a subject who is at risk of osteoporosis, which comprises detecting low BMD-associated aberration in a nucleic acid sample from the subject. An embodiment is a method for detecting a risk of osteoporosis in a subject, which comprises detecting the presence or absence of a polymorphic variation associated with low BMD at a polymorphic site in a nucleotide sequence in a nucleic acid sample from a subject, where the nucleotide sequence comprises a polynucleotide sequence selected from the group consisting of: (a) a nucleotide sequence of SEQ ID NO's:1-10; (b) a nucleotide sequence which encodes a polypeptide consisting of an amino acid sequence encoded by a nucleotide sequence of SEQ ID NO's:1-10; (c) a nucleotide sequence which encodes a polypeptide that is 90% or more identical to an amino acid sequence encoded by a nucleotide sequence of SEQ ID NO's:1-10, or a nucleotide sequence about 90% or more identical to a nucleotide sequence of SEQ ID NO's:1-10; and (d) a fragment of a nucleotide sequence of (a), (b), or (c) comprising the polymorphic site; whereby the presence of the polymorphic variation is indicative of a predisposition to osteoporosis in the subject. In certain embodiments, polymorphic variants at the positions described herein are detected for determining a risk of osteoporosis, and polymorphic variants at positions in linkage disequilibrium with these positions are detected for determining a risk of osteoporosis. As used herein, “SEQ ID NO's:1-10” refers to individual sequences in SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, and/or 12.

Results from prognostic tests may be combined with other test results to diagnose osteoporosis. For example, prognostic results may be gathered, an initial diagnostic test may be ordered based on a determined predisposition to low BMD, and the results of the analysis may be utilized to diagnose osteoporosis. Also osteoporosis diagnostic methods can be developed from studies used to generate prognostic/diagnostic methods in which populations are stratified into subpopulations having different progressions of osteoporosis. In another embodiment, prognostic results may be gathered; a patient's risk factors for developing osteoporosis analyzed (e.g., age, race, family history, age of menopause); and an initial diagnostic test may be ordered based on a determined predisposition to low BMD.

Risk factors believed to be associated with low BMD include personal history of fracture after age 50; current low bone mass; history of fracture in a 1° relative; being female; being thin and/or having a small frame; low body weight; advanced age; a family history of osteoporosis; estrogen deficiency, especially as a result of menopause which is early or surgically induced; abnormal absence of menstrual periods (amenorrhea); anorexia nervosa; low lifetime calcium intake; use of certain medications, such as corticosteroids and anticonvulsants; low testosterone levels in men; an inactive lifestyle; current cigarette smoking; excessive use of alcohol; being Caucasian or Asian, although African Americans and Hispanic Americans are at significant risk as well. (See National Osteoporosis Foundation; http://www.nof.org/osteoporosis/stats.htm). In an alternative embodiment, the results from predisposition analyses described herein may be combined with other test results indicative of osteoporosis, which were previously, concurrently, or subsequently gathered with respect to the predisposition testing. In these embodiments, the combination of the prognostic test results with other test results can be probative of osteoporosis, and the combination can be utilized as an osteoporosis diagnostic. The results of any test indicative of osteoporosis known in the art may be combined with the methods described herein. Examples of such tests are bone density tests that measure bone density in various sites of the body. Such methods include the measurement of the radiodensity of skeletal radiographs, quantitative computerized tomography, single energy photon absorptiometry, and dual-energy photon absorptiometry. Diagnostic techniques among those useful herein are described in W. A. Peck et al., Physician's Resource Manual on Osteoporosis (1987), published by the National Osteoporosis Foundation (incorporated by reference herein).

Risk of low BMD sometimes is expressed as a probability, such as an odds ratio, percentage, or risk factor. The risk sometimes is expressed as a relative risk with respect to a population average risk of low BMD, and sometimes is expressed as a relative risk with respect to the lowest risk group. Such relative risk assessments often are based upon penetrance values determined by statistical methods and are particularly useful to clinicians and insurance companies for assessing risk of osteoporosis (e.g., a clinician can target appropriate detection, prevention and therapeutic regimens to a patient after determining the patient's risk of osteoporosis, and an insurance company can fine tune actuarial tables based upon population genotype assessments of osteoporosis risk). Risk of osteoporosis sometimes is expressed as an odds ratio, which is the odds of a particular person having a genotype has or will develop osteoporosis with respect to another genotype group (e.g., the most disease protective genotype or population average). The risk often is based upon the presence or absence of one or more polymorphic variants described herein, and also may be based in part upon phenotypic traits of the individual being tested. In an embodiment, two or more polymorphic variations are detected in a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 locus. In certain embodiments, 3 or more, or 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20 or more polymorphic variants are detected in the sample. Methods for calculating risk based upon patient data are well known (see, e.g., Agresti, Categorical Data Analysis, 2nd Ed. 2002. Wiley). Allelotyping and genotyping analyses may be carried out in populations other than those exemplified herein to enhance the predictive power of the prognostic method.

The nucleic acid sample typically is isolated from a biological sample obtained from a subject. For example, nucleic acid can be isolated from blood, saliva, sputum, urine, cell scrapings, and biopsy tissue. The nucleic acid sample can be isolated from a biological sample using standard techniques, such as the technique described in Example 2. As used herein, the term “subject” refers primarily to humans but also refers to other mammals such as dogs, cats, and ungulates (e.g., cattle, sheep, and swine). Subjects also include avians (e.g., chickens and turkeys), reptiles, and fish (e.g., salmon), as embodiments described herein can be adapted to nucleic acid samples isolated from any of these organisms. The nucleic acid sample may be isolated from the subject and then directly utilized in a method for determining the presence of a polymorphic variant, or alternatively, the sample may be isolated and then stored (e.g., frozen) for a period of time before being subjected to analysis.

The presence or absence of a polymorphic variant is determined using one or both chromosomal complements represented in the nucleic acid sample. Determining the presence or absence of a polymorphic variant in both chromosomal complements represented in a nucleic acid sample from a subject having a copy of each chromosome is useful for determining the zygosity of an individual for the polymorphic variant (i.e., whether the individual is homozygous or heterozygous for the polymorphic variant). Any oligonucleotide-based diagnostic may be utilized to determine whether a sample includes the presence or absence of a polymorphic variant in a sample. For example, primer extension methods, ligase sequence determination methods (e.g., U.S. Pat. Nos. 5,679,524 and 5,952,174, and WO 01/27326), mismatch sequence determination methods (e.g., U.S. Pat. Nos. 5,851,770; 5,958,692; 6,110,684; and 6,183,958), microarray sequence determination methods, restriction fragment length polymorphism (RFLP), single strand conformation polymorphism detection (SSCP) (e.g., U.S. Pat. Nos. 5,891,625 and 6,013,499), PCR-based assays (e.g., TAQMAN® PCR System (Applied Biosystems)), and nucleotide sequencing methods may be used.

Oligonucleotide extension methods typically involve providing a pair of oligonucleotide primers in a polymerase chain reaction (PCR) or in other nucleic acid amplification methods for the purpose of amplifying a region from the nucleic acid sample that comprises the polymorphic variation. One oligonucleotide primer is complementary to a region 3′ of the polymorphism and the other is complementary to a region 5′ of the polymorphism. A PCR primer pair may be used in methods disclosed in U.S. Pat. Nos. 4,683,195; 4,683,202, 4,965,188; 5,656,493; 5,998,143; 6,140,054; WO 01/27327; and WO 01/27329 for example. PCR primer pairs may also be used in any commercially available machines that perform PCR, such as any of the GENEAMP® Systems available from Applied Biosystems. Also, those of ordinary skill in the art will be able to design oligonucleotide primers based upon a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence using knowledge available in the art.

Also provided is an extension oligonucleotide that hybridizes to the amplified fragment adjacent to the polymorphic variation. As used herein, the term “adjacent” refers to the 3′ end of the extension oligonucleotide being often 1 nucleotide from the 5′ end of the polymorphic site, and sometimes 2, 3, 4, 5, 6, 7, 8, 9, or 10 nucleotides from the 5′ end of the polymorphic site, in the nucleic acid when the extension oligonucleotide is hybridized to the nucleic acid. The extension oligonucleotide then is extended by one or more nucleotides, and the number and/or type of nucleotides that are added to the extension oligonucleotide determine whether the polymorphic variant is present. Oligonucleotide extension methods are disclosed, for example, in U.S. Pat. Nos. 4,656,127; 4,851,331; 5,679,524; 5,834,189; 5,876,934; 5,908,755; 5,912,118; 5,976,802; 5,981,186; 6,004,744; 6,013,431; 6,017,702; 6,046,005; 6,087,095; 6,210,891; and WO 01/20039. Oligonucleotide extension methods using mass spectrometry are described, for example, in U.S. Pat. Nos. 5,547,835; 5,605,798; 5,691,141; 5,849,542; 5,869,242; 5,928,906; 6,043,031; and 6,194,144, and a method often utilized is described herein in Example 2.

A microarray can be utilized for determining whether a polymorphic variant is present or absent in a nucleic acid sample. A microarray may include any oligonucleotides described herein, and methods for making and using oligonucleotide microarrays suitable for diagnostic use are disclosed in U.S. Pat. Nos. 5,492,806; 5,525,464; 5,589,330; 5,695,940; 5,849,483; 6,018,041; 6,045,996; 6,136,541; 6,142,681; 6,156,501; 6,197,506; 6,223,127; 6,225,625; 6,229,911; 6,239,273; WO 00/52625; WO 01/25485; and WO 01/29259. The microarray typically comprises a solid support and the oligonucleotides may be linked to this solid support by covalent bonds or by non-covalent interactions. The oligonucleotides may also be linked to the solid support directly or by a spacer molecule. A microarray may comprise one or more oligonucleotides complementary to a polymorphic site set forth herein.

A kit also may be utilized for determining whether a polymorphic variant is present or absent in a nucleic acid sample. A kit often comprises one or more pairs of oligonucleotide primers useful for amplifying a fragment of a nucleotide sequence of SEQ ID NO's:1-10 or a substantially identical sequence thereof, where the fragment includes a polymorphic site. The kit sometimes comprises a polymerizing agent, for example, a thermostable nucleic acid polymerase such as one disclosed in U.S. Pat. No. 4,889,818 or 6,077,664. Also, the kit often comprises an elongation oligonucleotide that hybridizes to a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence in a nucleic acid sample adjacent to the polymorphic site. Where the kit includes an elongation oligonucleotide, it also often comprises chain elongating nucleotides, such as dATP, dTTP, dGTP, dCTP, and dITP, including analogs of dATP, dTTP, dGTP, dCTP and dITP, provided that such analogs are substrates for a thermostable nucleic acid polymerase and can be incorporated into a nucleic acid chain elongated from the extension oligonucleotide. Along with chain elongating nucleotides would be one or more chain terminating nucleotides such as ddATP, ddTTP, ddGTP, ddCTP, and the like. In an embodiment, the kit comprises one or more oligonucleotide primer pairs, a polymerizing agent, chain elongating nucleotides, at least one elongation oligonucleotide, and one or more chain terminating nucleotides. Kits optionally include buffers, vials, microtiter plates, and instructions for use.

An individual identified as being at risk of osteoporosis may be heterozygous or homozygous with respect to the allele associated with low BMD. A subject homozygous for an allele associated with low BMD is at a comparatively high risk of osteoporosis, a subject heterozygous for an allele associated with low BMD is at a comparatively intermediate risk of osteoporosis, and a subject homozygous for an allele associated with normal or high BMD levels (i.e., a decreased risk of low BMD) is at a comparatively low risk of osteoporosis. A genotype may be assessed for a complementary strand, such that the complementary nucleotide at a particular position is detected.

Also featured are methods for determining risk of osteoporosis and/or identifying a subject at risk of osteoporosis by contacting a polypeptide or protein encoded by a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence from a subject with an antibody that specifically binds to an epitope associated with increased risk of osteoporosis in the polypeptide. In an embodiment, the antibody specifically binds to an epitope comprising an arginine at position 120 in a PROL4 polypeptide.

Applications of Prognostic and Diagnostic Results to Pharmacogenomic Methods

Pharmacogenomics is a discipline that involves tailoring a treatment for a subject according to the subject's genotype as a particular treatment regimen may exert a differential effect depending upon the subject's genotype. For example, based upon the outcome of a prognostic test described herein, a clinician or physician may target pertinent information and preventative or therapeutic treatments to a subject who would be benefited by the information or treatment and avoid directing such information and treatments to a subject who would not be benefited (e.g., the treatment has no therapeutic effect and/or the subject experiences adverse side effects).

The following is an example of a pharmacogenomic embodiment. A particular treatment regimen can exert a differential effect depending upon the subject's genotype. Where a candidate therapeutic exhibits a significant interaction with a major allele and a comparatively weak interaction with a minor allele (e.g., an order of magnitude or greater difference in the interaction), such a therapeutic typically would not be administered to a subject genotyped as being homozygous for the minor allele, and sometimes not administered to a subject genotyped as being heterozygous for the minor allele. In another example, where a candidate therapeutic is not significantly toxic when administered to subjects who are homozygous for a major allele but is comparatively toxic when administered to subjects heterozygous or homozygous for a minor allele, the candidate therapeutic is not typically administered to subjects who are genotyped as being heterozygous or homozygous with respect to the minor allele.

The methods described herein are applicable to pharmacogenomic methods for preventing, alleviating or treating low BMD conditions such as osteoporosis. For example, a nucleic acid sample from an individual may be subjected to a prognostic test described herein. Where one or more polymorphic variations associated with low BMD are identified in a subject, information for preventing or treating osteoporosis and/or one or more osteoporosis treatment regimens then may be prescribed to that subject.

In certain embodiments, a treatment or preventative regimen is specifically prescribed and/or administered to individuals who will most benefit from it based upon their risk of developing osteoporosis assessed by the methods described herein. Thus, provided are methods for identifying a subject predisposed to osteoporosis and then prescribing a therapeutic or preventative regimen to individuals identified as having a predisposition. Thus, certain embodiments are directed to a method for increasing BMD levels or otherwise treating osteoporosis in a subject, which comprises: detecting the presence or absence of a polymorphic variant associated with low BMD in a nucleotide sequence in a nucleic acid sample from a subject, where the nucleotide sequence comprises a polynucleotide sequence selected from the group consisting of: (a) a nucleotide sequence of SEQ ID NO's:1-10; (b) a nucleotide sequence which encodes a polypeptide consisting of an amino acid sequence encoded by a nucleotide sequence of SEQ ID NO's:1-10; (c) a nucleotide sequence which encodes a polypeptide that is 90% or more identical to an amino acid sequence encoded by a nucleotide sequence of SEQ ID NO's:1-10, or a nucleotide sequence about 90% or more identical to a nucleotide sequence of SEQ ID NO's:1-10; and (d) a fragment of a polynucleotide sequence of (a), (b), or (c); and prescribing or administering a treatment regimen to a subject from whom the sample originated where the presence of a polymorphic variation associated with low BMD is detected in the nucleotide sequence. In these methods, predisposition results may be utilized in combination with other test results to diagnose low BMD associated conditions, such as osteoporosis.

Certain preventative treatments often are prescribed to subjects having a predisposition to osteoporosis and where the subject is diagnosed with low BMD or is diagnosed as having symptoms indicative of early stage osteoporosis. Established diagnostic techniques use x-ray and ultrasonography to measure skeletal parameters of bone size, volume and mineral density to predict fracture risk and to assess response to therapy. Such measurements give a “static” value which can be compared to normal values to aid diagnosis of low bone mass and fracture risk (Schott, Cornier et al. 1998). The World Health Organization defines osteoporosis as a bone mineral density level more than 2.5 standard deviations below the young normal mean. The various techniques used to measure bone mineral density include, a) Dual energy X-ray absorptiometry (DXA)—used to measure bone mass at the lumbar spine and hip, but it can also be applied to measuring total skeletal bone mass, soft-tissue composition and other regional bone measurements. DXA is considered the “gold standard” for BMD measurement; b) high-resolution quantitative computed tomography QCT)—highly sensitive, accurate and specific spinal measurements. This technique is more costly, not widely available and involves higher radiation doses than other techniques; c) single-energy x-ray absorptiometry (SXA)—provides accurate radius BMD measurements; d) quantitative ultrasound QUS)—new and promising technique which may have applications in both BMD measurement and assessment or architectural deterioration of bone tissue. Recent studies suggest QUS of calcaneus bone predicts hip fracture as well as DXA (Hans, Dargent-Molina et al. 1996).

An alternative method to predict fracture independently of bone mass is to measure bone turnover. High turnover (bone resorption and formation) is associated with rapid bone loss and is likely to contribute to micro-architectural deterioration (Ross and Knowlton 1998). This is a “dynamic” measurement which is assessed with biochemical markers in urine or serum and can be used effectively in therapy monitoring in preference to BNO measurements. When used in combination with bone mass assessment, biomarkers can provide more accurate fracture predictions over bone mass measurement alone. Osteoporosis-related biomarkers for bone resorption include deoxypyridinoline crosslinks, and osteoporosis-related biomarkers for bone formation include bone alkaline phosphatase and osteocalcin. Some of these biomarkers have been developed for use in diagnostic kits. The current challenge is to reduce the variability of the measurements and improve their reliability and applicability.

The treatment sometimes is preventative (e.g., is prescribed or administered to reduce the probability that a low BMD associated condition arises or progresses), sometimes is therapeutic, and sometimes delays, alleviates or halts the progression of a low BMD associated condition. Any known preventative or therapeutic treatment for alleviating or preventing the occurrence of a low BMD associated disorder is prescribed and/or administered. Preventative treatment for osteoporosis is most effective at the time when bone loss is increasing and before the bones have become fragile and prone to fracturing. Strategies for the prevention of this disease include development of bone density in early adulthood (i.e., building strong bones during childhood and adolescence), and minimization of bone loss in later life. Changes in lifestyle, nutrition and hormonal factors have been shown to affect bone loss. Specifically, a balanced diet rich in calcium and vitamin D, weight-bearing exercise, a healthy lifestyle with no smoking or excessive alcohol intake, and bone density testing and medication when appropriate are known to help reduce the risk of osteoporosis.

As therapeutic approaches for low BMD continue to evolve and improve, the goal of treatments for low BMD related disorders is to intervene even before clinical signs (e.g., impaired glucose tolerance, or IGT) first manifest. Thus, genetic markers associated with susceptibility to low BMD prove useful for early diagnosis, prevention and treatment of low BMD.

As osteoporosis preventative and treatment information can be specifically targeted to subjects in need thereof (e.g., those at risk of low BMD or those that have early stages of osteoporosis), provided herein is a method for preventing or reducing the risk of developing osteoporosis in a subject, which comprises: (a) detecting the presence or absence of a polymorphic variation associated with low BMD at a polymorphic site in a nucleotide sequence in a nucleic acid sample from a subject; (b) identifying a subject with a predisposition to osteoporosis, whereby the presence of the polymorphic variation is indicative of a predisposition to osteoporosis in the subject; and (c) if such a predisposition is identified, providing the subject with information about methods or products to prevent osteoporosis or to delay the onset of osteoporosis. Also provided is a method of targeting information or advertising to a subpopulation of a human population based on the subpopulation being genetically predisposed to a disease or condition, which comprises: (a) detecting the presence or absence of a polymorphic variation associated with low BMD at a polymorphic site in a nucleotide sequence in a nucleic acid sample from a subject; (b) identifying the subpopulation of subjects in which the polymorphic variation is associated with low BMD; and (c) providing information only to the subpopulation of subjects about a particular product which may be obtained and consumed or applied by the subject to help prevent or delay onset of the disease or condition.

Pharmacogenomics methods also may be used to analyze and predict a response to a osteoporosis treatment or a drug. For example, if pharmacogenomics analysis indicates a likelihood that an individual will respond positively to a osteoporosis treatment with a particular drug, the drug may be administered to the individual. Conversely, if the analysis indicates that an individual is likely to respond negatively to treatment with a particular drug, an alternative course of treatment may be prescribed. A negative response may be defined as either the absence of an efficacious response or the presence of toxic side effects. The response to a therapeutic treatment can be predicted in a background study in which subjects in any of the following populations are genotyped: a population that responds favorably to a treatment regimen, a population that does not respond significantly to a treatment regimen, and a population that responds adversely to a treatment regiment (e.g., exhibits one or more side effects). These populations are provided as examples and other populations and subpopulations may be analyzed. Based upon the results of these analyses, a subject is genotyped to predict whether he or she will respond favorably to a treatment regimen, not respond significantly to a treatment regimen, or respond adversely to a treatment regimen.

The tests described herein also are applicable to clinical drug trials. One or more polymorphic variants indicative of response to an agent for treating osteoporosis or to side effects to an agent for treating osteoporosis may be identified using the methods described herein. Thereafter, potential participants in clinical trials of such an agent may be screened to identify those individuals most likely to respond favorably to the drug and exclude those likely to experience side effects. In that way, the effectiveness of drug treatment may be measured in individuals who respond positively to the drug, without lowering the measurement as a result of the inclusion of individuals who are unlikely to respond positively in the study and without risking undesirable safety problems.

Thus, another embodiment is a method of selecting an individual for inclusion in a clinical trial of a treatment or drug comprising the steps of: (a) obtaining a nucleic acid sample from an individual; (b) determining the identity of a polymorphic variation which is associated with a positive response to the treatment or the drug, or at least one polymorphic variation which is associated with a negative response to the treatment or the drug in the nucleic acid sample, and (c) including the individual in the clinical trial if the nucleic acid sample contains said polymorphic variation associated with a positive response to the treatment or the drug or if the nucleic acid sample lacks said polymorphic variation associated with a negative response to the treatment or the drug. In addition, the methods described herein for selecting an individual for inclusion in a clinical trial of a treatment or drug encompass methods with any further limitation described in this disclosure, or those following, specified alone or in any combination. The polymorphic variation may be in a sequence selected individually or in any combination from the group consisting of (i) a nucleotide sequence of SEQ ID NO's:1-10; (ii) a nucleotide sequence which encodes a polypeptide consisting of an amino acid sequence encoded by a nucleotide sequence of SEQ ID NO's:1-10; (iii) a nucleotide sequence which encodes a polypeptide that is 90% or more identical to an amino acid sequence encoded by a nucleotide sequence of SEQ ID NO's:1-10, or a nucleotide sequence about 90% or more identical to a nucleotide sequence of SEQ ID NO's:1-10; and (iv) a fragment of a polynucleotide sequence of (i), (ii), or (iii) comprising the polymorphic site. The including step (c) optionally comprises administering the drug or the treatment to the individual if the nucleic acid sample contains the polymorphic variation associated with a positive response to the treatment or the drug and the nucleic acid sample lacks said biallelic marker associated with a negative response to the treatment or the drug.

Also provided herein is a method of partnering between a diagnostic/prognostic testing provider and a provider of a consumable product, which comprises: (a) the diagnostic/prognostic testing provider detects the presence or absence of a polymorphic variation associated with low BMD at a polymorphic site in a nucleotide sequence in a nucleic acid sample from a subject; (b) the diagnostic/prognostic testing provider identifies the subpopulation of subjects in which the polymorphic variation is associated with low BMD; (c) the diagnostic/prognostic testing provider forwards information to the subpopulation of subjects about a particular product which may be obtained and consumed or applied by the subject to help prevent or delay onset of the disease or condition; and (d) the provider of a consumable product forwards to the diagnostic test provider a fee every time the diagnostic/prognostic test provider forwards information to the subject as set forth in step (c) above.

Compositions Comprising Osteoporosis-Directed Molecules

Featured herein is a composition comprising a cell from a subject having low BMD or at risk of osteoporosis and one or more molecules specifically directed and targeted to a nucleic acid comprising a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence or amino acid sequence. Such directed molecules include, but are not limited to, a compound that binds to a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence or amino acid sequence referenced herein; a nucleic acid that hybridizes to a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleic acid under conditions of high stringency; a RNAi or siRNA molecule having a strand complementary to a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence; an antisense nucleic acid complementary to an RNA encoded by a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence; a ribozyme that hybridizes to a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence; a nucleic acid aptamer that specifically binds a polypeptide encoded by CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence; and an antibody that specifically binds to a polypeptide encoded by CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence or binds to a nucleic acid having such a nucleotide sequence. In specific embodiments, the osteoporosis directed molecule interacts with a nucleic acid or polypeptide variant associated with osteoporosis, such as variants referenced herein. In other embodiments, the osteoporosis directed molecule interacts with a polypeptide involved in a signal pathway of a polypeptide encoded by a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence, or a nucleic acid comprising such a nucleotide sequence.

Compositions sometimes include an adjuvant known to stimulate an immune response, and in certain embodiments, an adjuvant that stimulates a T-cell lymphocyte response. Adjuvants are known, including but not limited to an aluminum adjuvant (e.g., aluminum hydroxide); a cytokine adjuvant or adjuvant that stimulates a cytokine response (e.g., interleukin (IL)-12 and/or γ-interferon cytokines); a Freund-type mineral oil adjuvant emulsion (e.g., Freund's complete or incomplete adjuvant); a synthetic lipoid compound; a copolymer adjuvant (e.g., TitreMax); a saponin; Quil A; a liposome; an oil-in-water emulsion (e.g., an emulsion stabilized by Tween 80 and pluronic polyoxyethlene/polyoxypropylene block copolymer (Syntex Adjuvant Formulation); TitreMax; detoxified endotoxin (MPL) and mycobacterial cell wall components (TDW, CWS) in 2% squalene (Ribi Adjuvant System)); a muramyl dipeptide; an immune-stimulating complex (ISCOM, e.g., an Ag-modified saponin/cholesterol micelle that forms stable cage-like structure); an aqueous phase adjuvant that does not have a depot effect (e.g., Gerbu adjuvant); a carbohydrate polymer (e.g., AdjuPrime); L-tyrosine; a manide-oleate compound (e.g., Montanide); an ethylene-vinyl acetate copolymer (e.g., Elvax 40W1,2); or lipid A, for example. Such compositions are useful for generating an immune response against a osteoporosis directed molecule (e.g., an HLA-binding subsequence within a polypeptide encoded by a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence). In such methods, a peptide having an amino acid subsequence of a polypeptide encoded by a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence is delivered to a subject, where the subsequence binds to an HLA molecule and induces a CTL lymphocyte response. The peptide sometimes is delivered to the subject as an isolated peptide or as a minigene in a plasmid that encodes the peptide. Methods for identifying HLA-binding subsequences in such polypeptides are known (see e.g., publication WO02/20616 and PCT application US98/01373 for methods of identifying such sequences).

The cell may be in a group of cells cultured in vitro or in a tissue maintained in vitro or present in an animal in vivo (e.g., a rat, mouse, ape or human). In certain embodiments, a composition comprises a component from a cell such as a nucleic acid molecule (e.g., genomic DNA), a protein mixture or isolated protein, for example. The aforementioned compositions have utility in diagnostic, prognostic and pharmacogenomic methods described previously and in osteoporosis therapeutics described hereafter. Certain osteoporosis directed molecules are described in greater detail below.

Compounds

Compounds can be obtained using any of the numerous approaches in combinatorial library methods known in the art, including: biological libraries; peptoid libraries (libraries of molecules having the functionalities of peptides, but with a novel, non-peptide backbone which are resistant to enzymatic degradation but which nevertheless remain bioactive (see, e.g., Zuckermann et al., J. Med. Chem. 37: 2678-85 (1994)); spatially addressable parallel solid phase or solution phase libraries; synthetic library methods requiring deconvolution; “one-bead one-compound” library methods; and synthetic library methods using affinity chromatography selection. Biological library and peptoid library approaches are typically limited to peptide libraries, while the other approaches are applicable to peptide, non-peptide oligomer or small molecule libraries of compounds (Lam, Anticancer Drug Des. 12: 145, (1997)). Examples of methods for synthesizing molecular libraries are described, for example, in DeWitt et al., Proc. Natl. Acad. Sci. U.S.A. 90: 6909 (1993); Erb et al., Proc. Natl. Acad. Sci. USA 91: 11422 (1994); Zuckermann et al., J. Med. Chem. 37: 2678 (1994); Cho et al., Science 261: 1303 (1993); Carrell et al., Angew. Chem. Int. Ed. Engl. 33: 2059 (1994); Carell et al., Angew. Chem. Int. Ed. Engl. 33: 2061 (1994); and in Gallop et al., J. Med. Chem. 37: 1233 (1994).

Libraries of compounds may be presented in solution (e.g., Houghten, Biotechniques 13: 412-421 (1992)), or on beads (Lam, Nature 354: 82-84 (1991)), chips (Fodor, Nature 364: 555-556 (1993)), bacteria or spores (Ladner, U.S. Pat. No. 5,223,409), plasmids (Cull et al., Proc. Natl. Acad. Sci. USA 89: 1865-1869 (1992)) or on phage (Scott and Smith, Science 249: 386-390 (1990); Devlin, Science 249: 404-406 (1990); Cwirla et al., Proc. Natl. Acad. Sci. 87: 6378-6382 (1990); Felici, J. Mol. Biol. 222: 301-310 (1991); Ladner supra.).

A compound sometimes alters expression and sometimes alters activity of a polypeptide target and may be a small molecule. Small molecules include, but are not limited to, peptides, peptidomimetics (e.g., peptoids), amino acids, amino acid analogs, polynucleotides, polynucleotide analogs, nucleotides, nucleotide analogs, organic or inorganic compounds (i.e., including heteroorganic and organometallic compounds) having a molecular weight less than about 10,000 grams per mole, organic or inorganic compounds having a molecular weight less than about 5,000 grams per mole, organic or inorganic compounds having a molecular weight less than about 1,000 grams per mole, organic or inorganic compounds having a molecular weight less than about 500 grams per mole, and salts, esters, and other pharmaceutically acceptable forms of such compounds.

Antisense Nucleic Acid Molecules, Ribozymes, RNAi, siRNA and Modified Nucleic Acid Molecules

An “antisense” nucleic acid refers to a nucleotide sequence complementary to a “sense” nucleic acid encoding a polypeptide, e.g., complementary to the coding strand of a double-stranded cDNA molecule or complementary to an mRNA sequence. The antisense nucleic acid can be complementary to an entire coding strand (e.g., SEQ ID NO: 7-13), or to a portion thereof or a substantially identical sequence thereof. In another embodiment, the antisense nucleic acid molecule is antisense to a “noncoding region” of the coding strand of a nucleotide sequence (e.g., 5′ and 3′ untranslated regions in SEQ ID NO's:1-5).

An antisense nucleic acid can be designed such that it is complementary to the entire coding region of an mRNA encoded by a nucleotide sequence (e.g., SEQ ID NO's:1-10), and often the antisense nucleic acid is an oligonucleotide antisense to only a portion of a coding or noncoding region of the mRNA. For example, the antisense oligonucleotide can be complementary to the region surrounding the translation start site of the mRNA, e.g., between the −10 and +10 regions of the target gene nucleotide sequence of interest. An antisense oligonucleotide can be, for example, about 7, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, or more nucleotides in length. The antisense nucleic acids, which include the ribozymes described hereafter, can be designed to target a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence, often a variant associated with low BMD, or a substantially identical sequence thereof. Among the variants, minor alleles and major alleles can be targeted, and those associated with a higher risk of osteoporosis are often designed, tested, and administered to subjects.

An antisense nucleic acid can be constructed using chemical synthesis and enzymatic ligation reactions using standard procedures. For example, an antisense nucleic acid (e.g., an antisense oligonucleotide) can be chemically synthesized using naturally occurring nucleotides or variously modified nucleotides designed to increase the biological stability of the molecules or to increase the physical stability of the duplex formed between the antisense and sense nucleic acids, e.g., phosphorothioate derivatives and acridine substituted nucleotides can be used. Antisense nucleic acid also can be produced biologically using an expression vector into which a nucleic acid has been subcloned in an antisense orientation (i.e., RNA transcribed from the inserted nucleic acid will be of an antisense orientation to a target nucleic acid of interest, described further in the following subsection).

When utilized as therapeutics, antisense nucleic acids typically are administered to a subject (e.g., by direct injection at a tissue site) or generated in situ such that they hybridize with or bind to cellular mRNA and/or genomic DNA encoding a polypeptide and thereby inhibit expression of the polypeptide, for example, by inhibiting transcription and/or translation. Alternatively, antisense nucleic acid molecules can be modified to target selected cells and then are administered systemically. For systemic administration, antisense molecules can be modified such that they specifically bind to receptors or antigens expressed on a selected cell surface, for example, by linking antisense nucleic acid molecules to peptides or antibodies which bind to cell surface receptors or antigens. Antisense nucleic acid molecules can also be delivered to cells using the vectors described herein. Sufficient intracellular concentrations of antisense molecules are achieved by incorporating a strong promoter, such as a pol II or pol III promoter, in the vector construct.

Antisense nucleic acid molecules sometimes are *-anomeric nucleic acid molecules. An *-anomeric nucleic acid molecule forms specific double-stranded hybrids with complementary RNA in which, contrary to the usual *-units, the strands run parallel to each other (Gaultier et al., Nucleic Acids. Res. 15: 6625-6641 (1987)). Antisense nucleic acid molecules can also comprise a 2′-o-methylribonucleotide (Inoue et al., Nucleic Acids Res. 15: 6131-6148 (1987)) or a chimeric RNA-DNA analogue (Inoue et al., FEBS Lett. 215: 327-330 (1987)). Antisense nucleic acids sometimes are composed of DNA or PNA or any other nucleic acid derivatives described previously.

In another embodiment, an antisense nucleic acid is a ribozyme. A ribozyme having specificity for a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence can include one or more sequences complementary to such a nucleotide sequence, and a sequence having a known catalytic region responsible for mRNA cleavage (see e.g., U.S. Pat. No. 5,093,246 or Haselhoff and Gerlach, Nature 334: 585-591 (1988)). For example, a derivative of a Tetrahymena L-19 IVS RNA is sometimes utilized in which the nucleotide sequence of the active site is complementary to the nucleotide sequence to be cleaved in a mRNA (see e.g., Cech et al. U.S. Pat. No. 4,987,071; and Cech et al. U.S. Pat. No. 5,116,742). Also, target mRNA sequences can be used to select a catalytic RNA having a specific ribonuclease activity from a pool of RNA molecules (see e.g., Bartel & Szostak, Science 261: 1411-1418(1993)).

Osteoporosis directed molecules include in certain embodiments nucleic acids that can form triple helix structures with a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence or a substantially identical sequence thereof, especially one that includes a regulatory region that controls expression of a polypeptide. Gene expression can be inhibited by targeting nucleotide sequences complementary to the regulatory region of a nucleotide sequence referenced herein or a substantially identical sequence (e.g., promoter and/or enhancers) to form triple helical structures that prevent transcription of a gene in target cells (see e.g., Helene, Anticancer Drug Des. 6(6): 569-84 (1991); Helene et al., Ann. N.Y. Acad. Sci. 660: 27-36 (1992); and Maher, Bioassays 14(12): 807-15 (1992). Potential sequences that can be targeted for triple helix formation can be increased by creating a so-called “switchback” nucleic acid molecule. Switchback molecules are synthesized in an alternating 5′-3′, 3′-5′ manner, such that they base pair with first one strand of a duplex and then the other, eliminating the necessity for a sizeable stretch of either purines or pyrimidines to be present on one strand of a duplex.

Osteoporosis directed molecules include RNAi and siRNA nucleic acids. Gene expression may be inhibited by the introduction of double-stranded RNA (dsRNA), which induces potent and specific gene silencing, a phenomenon called RNA interference or RNAi. See, e.g., Fire et al., U.S. Pat. No. 6,506,559; Tuschl et al. PCT International Publication No. WO 01/75164; Kay et al. PCT International Publication No. WO 03/010180A1; or Bosher J M, Labouesse, Nat Cell Biol 2000 February; 2(2):E31-6. This process has been improved by decreasing the size of the double-stranded RNA to 20-24 base pairs (to create small-interfering RNAs or siRNAs) that “switched off” genes in mammalian cells without initiating an acute phase response, i.e., a host defense mechanism that often results in cell death (see, e.g., Caplen et al. Proc Natl Acad Sci USA. 2001 Aug. 14; 98(17):9742-7 and Elbashir et al. Methods 2002 February; 26(2): 199-213). There is increasing evidence of post-transcriptional gene silencing by RNA interference (RNAi) for inhibiting targeted expression in mammalian cells at the mRNA level, in human cells. There is additional evidence of effective methods for inhibiting the proliferation and migration of tumor cells in human patients, and for inhibiting metastatic cancer development (see, e.g., U.S. Patent Application No. US2001000993183; Caplen et al. Proc Natl Acad Sci USA; and Abdeffahmani et al. Mol Cell Biol 2001 November 21(21):7256-67).

An “siRNA” or “RNAi” refers to a nucleic acid that forms a double stranded RNA and has the ability to reduce or inhibit expression of a gene or target gene when the siRNA is delivered to or expressed in the same cell as the gene or target gene. “siRNA” refers to short double-stranded RNA formed by the complementary strands. Complementary portions of the siRNA that hybridize to form the double stranded molecule often have substantial or complete identity to the target molecule sequence. In one embodiment, an siRNA refers to a nucleic acid that has substantial or complete identity to a target gene and forms a double stranded siRNA.

When designing the siRNA molecules, the targeted region often is selected from a given DNA sequence beginning 50 to 100 nucleotides downstream of the start codon. See, e.g., Elbashir et al., Methods 26:199-213 (2002). Initially, 5′ or 3′ UTRs and regions nearby the start codon were avoided assuming that UTR-binding proteins and/or translation initiation complexes may interfere with binding of the siRNP or RISC endonuclease complex. Sometimes regions of the target 23 nucleotides in length conforming to the sequence motif AA(N19)TT (N, an nucleotide), and regions with approximately 30% to 70% G/C-content (often about 50% G/C-content) often are selected. If no suitable sequences are found, the search often is extended using the motif NA(N21). The sequence of the sense siRNA sometimes corresponds to (N19) TT or N21 (position 3 to 23 of the 23-nt motif), respectively. In the latter case, the 3′ end of the sense siRNA often is converted to TT. The rationale for this sequence conversion is to generate a symmetric duplex with respect to the sequence composition of the sense and antisense 3′ overhangs. The antisense siRNA is synthesized as the complement to position 1 to 21 of the 23-nt motif. Because position 1 of the 23-nt motif is not recognized sequence-specifically by the antisense siRNA, the 3′-most nucleotide residue of the antisense siRNA can be chosen deliberately. However, the penultimate nucleotide of the antisense siRNA (complementary to position 2 of the 23-nt motif) often is complementary to the targeted sequence. For simplifying chemical synthesis, TT often is utilized. siRNAs corresponding to the target motif NAR(N17)YNN, where R is purine (A,G) and Y is pyrimidine (C,U), often are selected. Respective 21 nucleotide sense and antisense siRNAs often begin with a purine nucleotide and can also be expressed from pol III expression vectors without a change in targeting site. Expression of RNAs from pol III promoters often is efficient when the first transcribed nucleotide is a purine.

The sequence of the siRNA can correspond to the full length target gene, or a subsequence thereof. Often, the siRNA is about 15 to about 50 nucleotides in length (e.g., each complementary sequence of the double stranded siRNA is 15-50 nucleotides in length, and the double stranded siRNA is about 15-50 base pairs in length, sometimes about 20-30 nucleotides in length or about 20-25 nucleotides in length, e.g., 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 nucleotides in length. The siRNA sometimes is about 21 nucleotides in length. Methods of using siRNA are well known in the art, and specific siRNA molecules may be purchased from a number of companies including Dharmacon Research, Inc. An siRNA molecule sometimes is of a different chemical composition as compared to native RNA that imparts increased stability in cells (e.g., decreased susceptibility to degradation), and sometimes includes one or more modifications in siSTABLE RNA described at the http address www.dharmacon.com.

Antisense, ribozyme, RNAi and siRNA nucleic acids can be altered to form modified nucleic acid molecules. The nucleic acids can be altered at base moieties, sugar moieties or phosphate backbone moieties to improve stability, hybridization, or solubility of the molecule. For example, the deoxyribose phosphate backbone of nucleic acid molecules can be modified to generate peptide nucleic acids (see Hyrup et al., Bioorganic & Medicinal Chemistry 4 (1): 5-23 (1996)). As used herein, the terms “peptide nucleic acid” or “PNA” refers to a nucleic acid mimic such as a DNA mimic, in which the deoxyribose phosphate backbone is replaced by a pseudopeptide backbone and only the four natural nucleobases are retained. The neutral backbone of a PNA can allow for specific hybridization to DNA and RNA under conditions of low ionic strength. Synthesis of PNA oligomers can be performed using standard solid phase peptide synthesis protocols as described, for example, in Hyrup et al., (1996) supra and Perry-O'Keefe et al., Proc. Natl. Acad. Sci. 93: 14670-675 (1996).

PNA nucleic acids can be used in prognostic, diagnostic, and therapeutic applications. For example, PNAs can be used as antisense or antigene agents for sequence-specific modulation of gene expression by, for example, inducing transcription or translation arrest or inhibiting replication. PNA nucleic acid molecules can also be used in the analysis of single base pair mutations in a gene, (e.g., by PNA-directed PCR clamping); as “artificial restriction enzymes” when used in combination with other enzymes, (e.g., S1 nucleases (Hyrup (1996) supra)); or as probes or primers for DNA sequencing or hybridization (Hyrup et al., (1996) supra; Perry-O'Keefe supra).

In other embodiments, oligonucleotides may include other appended groups such as peptides (e.g., for targeting host cell receptors in vivo), or agents facilitating transport across cell membranes (see e.g., Letsinger et al., Proc. Natl. Acad. Sci. USA 86: 6553-6556 (1989); Lemaitre et al., Proc. Natl. Acad. Sci. USA 84: 648-652 (1987); PCT Publication No. WO88/09810) or the blood-brain barrier (see, e.g., PCT Publication No. WO 89/10134). In addition, oligonucleotides can be modified with hybridization-triggered cleavage agents (See, e.g., Krol et al., Bio-Techniques 6: 958-976 (1988)) or intercalating agents. (See, e.g., Zon, Pharm. Res. 5: 539-549 (1988)). To this end, the oligonucleotide may be conjugated to another molecule, (e.g., a peptide, hybridization triggered cross-linking agent, transport agent, or hybridization-triggered cleavage agent).

Also included herein are molecular beacon oligonucleotide primer and probe molecules having one or more regions complementary to a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence or a substantially identical sequence thereof, two complementary regions one having a fluorophore and one a quencher such that the molecular beacon is useful for quantifying the presence of the nucleic acid in a sample. Molecular beacon nucleic acids are described, for example, in Lizardi et al., U.S. Pat. No. 5,854,033; Nazarenko et al., U.S. Pat. No. 5,866,336, and Livak et al., U.S. Pat. No. 5,876,930.

Antibody

The term “antibody” as used herein refers to an immunoglobulin molecule or immunologically active portion thereof, i.e., an antigen-binding portion. Examples of immunologically active portions of immunoglobulin molecules include F(ab) and F(ab′)₂ fragments which can be generated by treating the antibody with an enzyme such as pepsin. An antibody sometimes is a polyclonal, monoclonal, recombinant (e.g., a chimeric or humanized), fully human, non-human (e.g., murine), or a single chain antibody. An antibody may have effector function and can fix complement, and is sometimes coupled to a toxin or imaging agent.

Antibodies

A full-length polypeptide or antigenic peptide fragment encoded by a nucleotide sequence referenced herein can be used as an immunogen or can be used to identify antibodies made with other immunogens, e.g., cells, membrane preparations, and the like. An antigenic peptide often includes at least 8 amino acid residues of the amino acid sequences encoded by a nucleotide sequence referenced herein, or substantially identical sequence thereof, and encompasses an epitope. Antigenic peptides sometimes include 10 or more amino acids, 15 or more amino acids, 20 or more amino acids, or 30 or more amino acids. Hydrophilic and hydrophobic fragments of polypeptides sometimes are used as immunogens.

Epitopes encompassed by the antigenic peptide are regions located on the surface of the polypeptide (e.g., hydrophilic regions) as well as regions with high antigenicity. For example, an Emini surface probability analysis of the human polypeptide sequence can be used to indicate the regions that have a particularly high probability of being localized to the surface of the polypeptide and are thus likely to constitute surface residues useful for targeting antibody production. The antibody may bind an epitope on any domain or region on polypeptides described herein.

Also, chimeric, humanized, and completely human antibodies are useful for applications which include repeated administration to subjects. Chimeric and humanized monoclonal antibodies, comprising both human and non-human portions, can be made using standard recombinant DNA techniques. Such chimeric and humanized monoclonal antibodies can be produced by recombinant DNA techniques known in the art, for example using methods described in Robinson et al International Application No. PCT/US86/02269; Akira, et al European Patent Application 184,187; Taniguchi, M., European Patent Application 171,496; Morrison et al European Patent Application 173,494; Neuberger et al PCT International Publication No. WO 86/01533; Cabilly et al U.S. Pat. No. 4,816,567; Cabilly et al European Patent Application 125,023; Better et al., Science 240: 1041-1043 (1988); Liu et al., Proc. Natl. Acad. Sci. USA 84: 3439-3443 (1987); Liu et al., J. Immunol. 139: 3521-3526 (1987); Sun et al., Proc. Natl. Acad. Sci. USA 84: 214-218 (1987); Nishimura et al., Canc. Res. 47: 999-1005 (1987); Wood et al., Nature 314: 446-449 (1985); and Shaw et al., J. Natl. Cancer Inst. 80: 1553-1559 (1988); Morrison, S. L., Science 229:1202-1207(1985); Oi et al., BioTechniques 4: 214(1986); Winter U.S. Pat. No. 5,225,539; Jones et al., Nature 321: 552-525 (1986); Verhoeyan et al., Science 239: 1534; and Beidler et al., J. Immunol. 141: 4053-4060 (1988).

Completely human antibodies are particularly desirable for therapeutic treatment of human patients. Such antibodies can be produced using transgenic mice that are incapable of expressing endogenous immunoglobulin heavy and light chains genes, but which can express human heavy and light chain genes. See, for example, Lonberg and Huszar, Int. Rev. Immunol. 13: 65-93 (1995); and U.S. Pat. Nos. 5,625,126; 5,633,425; 5,569,825; 5,661,016; and 5,545,806. In addition, companies such as Abgenix, Inc. (Fremont, Calif.) and Medarex, Inc. (Princeton, N.J.), can be engaged to provide human antibodies directed against a selected antigen using technology similar to that described above. Completely human antibodies that recognize a selected epitope also can be generated using a technique referred to as “guided selection.” In this approach a selected non-human monoclonal antibody (e.g., a murine antibody) is used to guide the selection of a completely human antibody recognizing the same epitope. This technology is described for example by Jespers et al., Bio/Technology 12: 899-903 (1994).

An antibody can be a single chain antibody. A single chain antibody (scFV) can be engineered (see, e.g., Colcher et al., Ann. N Y Acad. Sci. 880: 263-80 (1999); and Reiter, Clin. Cancer Res. 2: 245-52 (1996)). Single chain antibodies can be dimerized or multimerized to generate multivalent antibodies having specificities for different epitopes of the same target polypeptide.

Antibodies also may be selected or modified so that they exhibit reduced or no ability to bind an Fc receptor. For example, an antibody may be an isotype or subtype, fragment or other mutant, which does not support binding to an Fc receptor (e.g., it has a mutagenized or deleted Fc receptor binding region).

Also, an antibody (or fragment thereof) may be conjugated to a therapeutic moiety such as a cytotoxin, a therapeutic agent or a radioactive metal ion. A cytotoxin or cytotoxic agent includes any agent that is detrimental to cells. Examples include taxol, cytochalasin B, gramicidin D, ethidium bromide, emetine, mitomycin, etoposide, tenoposide, vincristine, vinblastine, colchicin, doxorubicin, daunorubicin, dihydroxy anthracin dione, mitoxantrone, mithramycin, actinomycin D, l dehydrotestosterone, glucocorticoids, procaine, tetracaine, lidocaine, propranolol, and puromycin and analogs or homologs thereof. Therapeutic agents include, but are not limited to, antimetabolites (e.g., methotrexate, 6-mercaptopurine, 6-thioguanine, cytarabine, 5-fluorouracil decarbazine), alkylating agents (e.g., mechlorethamine, thiotepa chlorambucil, melphalan, carmustine (BCNU) and lomustine (CCNU), cyclophosphamide, busulfan, dibromomannitol, streptozotocin, mitomycin C, and cis-dichlorodiamine platinum (II) (DDP) cisplatin), anthracyclines (e.g., daunorubicin (formerly daunomycin) and doxorubicin), antibiotics (e.g., dactinomycin (formerly actinomycin), bleomycin, mithramycin, and anthramycin (AMC)), and anti-mitotic agents (e.g., vincristine and vinblastine).

Antibody conjugates can be used for modifying a given biological response. For example, the drug moiety may be a protein or polypeptide possessing a desired biological activity. Such proteins may include, for example, a toxin such as abrin, ricin A, pseudomonas exotoxin, or diphtheria toxin; a polypeptide such as tumor necrosis factor, γ-interferon, α-interferon, nerve growth factor, platelet derived growth factor, tissue plasminogen activator; or, biological response modifiers such as, for example, lymphokines, interleukin-1 (“IL-1”), interleukin-2 (“IL-2”), interleukin-6 (“IL-6”), granulocyte macrophage colony stimulating factor (“GM-CSF”), granulocyte colony stimulating factor “G-CSF”), or other growth factors. Also, an antibody can be conjugated to a second antibody to form an antibody heteroconjugate as described by Segal in U.S. Pat. No. 4,676,980, for example.

An antibody (e.g., monoclonal antibody) can be used to isolate target polypeptides by standard techniques, such as affinity chromatography or immunoprecipitation. Moreover, an antibody can be used to detect a target polypeptide (e.g., in a cellular lysate or cell supernatant) in order to evaluate the abundance and pattern of expression of the polypeptide. Antibodies can be used diagnostically to monitor polypeptide levels in tissue as part of a clinical testing procedure, e.g., to determine the efficacy of a given treatment regimen. Detection can be facilitated by coupling (i.e., physically linking) the antibody to a detectable substance (i.e., antibody labeling). Examples of detectable substances include various enzymes, prosthetic groups, fluorescent materials, luminescent materials, bioluminescent materials, and radioactive materials. Examples of suitable enzymes include horseradish peroxidase, alkaline phosphatase, β-galactosidase, or acetylcholinesterase; examples of suitable prosthetic group complexes include streptavidin/biotin and avidin/biotin; examples of suitable fluorescent materials include umbelliferone, fluorescein, fluorescein isothiocyanate, rhodamine, dichlorotriazinylamine fluorescein, dansyl chloride or phycoerythrin; an example of a luminescent material includes luminol; examples of bioluminescent materials include luciferase, luciferin, and aequorin, and examples of suitable radioactive material include ¹²⁵I, ¹³¹I, ³⁵S or ³H. Also, an antibody can be utilized as a test molecule for determining whether it can treat osteoporosis, and as a therapeutic for administration to a subject for treating osteoporosis.

An antibody can be made by immunizing with a purified antigen, or a fragment thereof, e.g., a fragment described herein, a membrane associated antigen, tissues, e.g., crude tissue preparations, whole cells, preferably living cells, lysed cells, or cell fractions.

Included herein are antibodies which bind only a native polypeptide, only denatured or otherwise non-native polypeptide, or which bind both, as well as those having linear or conformational epitopes. Conformational epitopes sometimes can be identified by selecting antibodies that bind to native but not denatured polypeptide. Also featured are antibodies that specifically bind to a polypeptide variant associated with low BMD.

Methods for Identifying Candidate Therapeutics for Treating Osteoporosis

Current treatment for osteoporosis can help stop further bone loss and fractures, but there is still a clear need for specific anabolic agents that considerably increase bone formation in people who have already suffered substantial bone loss. There are no such drugs currently approved. Known osteoporosis treatments include bone-active phosphonates (See, e.g., U.S. Pat. No. 6,329,354), bisphosphonates, e.g., alendronate (Fosamax®) and risedronate (Actonel®); calcitonin (Niacalcin®); estrogen and hormone therapy, e.g., estrogens (Climara®, Estrace®, Estraderm®, Estratab®, Ogen®, Ortho-Est®, Vivelle®, Premarin®, and others) and estrogens and progestins (brand names, such as Activella™, FemHrt®, Premphase®, Prempro®, and others); parathyroid hormone, e.g., teriparatide {PTH 1-34} (Forteo®); as well as, estrogen and estrogen receptor modulators (SERMs), e.g., raloxifene HCl (Evista®), sodium fluoride and vitamin D metabolites. Any of the above therapeutics may be administered alone or in combination (e.g., bone-active phosphonates and estrogen and hormone therapy). Current therapeutic approaches were largely developed in the absence of defined molecular targets or even a solid understanding of disease pathogenesis. Therefore, provided are methods of identifying candidate therapeutics that target biochemical pathways related to the development of osteoporosis.

Thus, featured herein are methods for identifying a candidate therapeutic for treating osteoporosis. The methods comprise contacting a test molecule with a target molecule in a system. A “target molecule” as used herein refers to a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleic acid, a substantially identical nucleic acid thereof, or a fragment thereof, and an encoded polypeptide of the foregoing. The methods also comprise determining the presence or absence of an interaction between the test molecule and the target molecule, where the presence of an interaction between the test molecule and the nucleic acid or polypeptide identifies the test molecule as a candidate osteoporosis therapeutic. The interaction between the test molecule and the target molecule may be quantified.

Test molecules and candidate therapeutics include, but are not limited to, compounds, antisense nucleic acids, siRNA molecules, ribozymes, polypeptides or proteins encoded by a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence, or a substantially identical sequence or fragment thereof, and immunotherapeutics (e.g., antibodies and HLA-presented polypeptide fragments). A test molecule or candidate therapeutic may act as a modulator of target molecule concentration or target molecule function in a system. A “modulator” may agonize (i.e., up-regulates) or antagonize (i.e., down-regulates) a target molecule concentration partially or completely in a system by affecting such cellular functions as DNA replication and/or DNA processing (e.g. DNA methylation or DNA repair), RNA transcription and/or RNA processing (e.g., removal of intronic sequences and/or translocation of spliced mRNA from the nucleus), polypeptide production (e.g., translation of the polypeptide from mRNA), and/or polypeptide post-translational modification (e.g., glycosylation, phosphorylation, and proteolysis of pro-polypeptides). A modulator may also agonize or antagonize a biological function of a target molecule partially or completely, where the function may include adopting a certain structural conformation, interacting with one or more binding partners, ligand binding, catalysis (e.g., phosphorylation, dephosphorylation, hydrolysis, methylation, and isomerization), and an effect upon a cellular event (e.g., effecting progression of osteoporosis). In certain embodiments, a candidate therapeutic increases BMD.

As used herein, the term “system” refers to a cell free in vitro environment and a cell-based environment such as a collection of cells, a tissue, an organ, or an organism. A system is “contacted” with a test molecule in a variety of manners, including adding molecules in solution and allowing them to interact with one another by diffusion, cell injection, and any administration routes in an animal. As used herein, the term “interaction” refers to an effect of a test molecule on test molecule, where the effect sometimes is binding between the test molecule and the target molecule, and sometimes is an observable change in cells, tissue, or organism.

There are many standard methods for detecting the presence or absence of interaction between a test molecule and a target molecule. For example, titrametric, acidimetric, radiometric, NMR, monolayer, polarographic, spectrophotometric, fluorescent, and ESR assays probative of a target molecule interaction may be utilized.

Test molecule/target molecule interactions can be detected and/or quantified using assays known in the art. For example, an interaction can be determined by labeling the test molecule and/or the target molecule, where the label is covalently or non-covalently attached to the test molecule or target molecule. The label is sometimes a radioactive molecule such as ¹²⁵I, ¹³¹I, ³⁵S or ³H, which can be detected by direct counting of radioemission or by scintillation counting. Also, enzymatic labels such as horseradish peroxidase, alkaline phosphatase, or luciferase may be utilized where the enzymatic label can be detected by determining conversion of an appropriate substrate to product. In addition, presence or absence of an interaction can be determined without labeling. For example, a microphysiometer (e.g., Cytosensor) is an analytical instrument that measures the rate at which a cell acidifies its environment using a light-addressable potentiometric sensor (LAPS). Changes in this acidification rate can be used as an indication of an interaction between a test molecule and target molecule (McConnell, H. M. et al., Science 257: 1906-1912 (1992)).

In cell-based systems, cells typically include a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleic acid, an encoded polypeptide, or substantially identical nucleic acid or polypeptide thereof, and are often of mammalian origin, although the cell can be of any origin. Whole cells, cell homogenates, and cell fractions (e.g., cell membrane fractions) can be subjected to analysis. Where interactions between a test molecule with a target polypeptide are monitored, soluble and/or membrane bound forms of the polypeptide may be utilized. Where membrane-bound forms of the polypeptide are used, it may be desirable to utilize a solubilizing agent. Examples of such solubilizing agents include non-ionic detergents such as n-octylglucoside, n-dodecylglucoside, n-dodecylmaltoside, octanoyl-N-methylglucamide, decanoyl-N-methylglucamide, Triton® X-100, Triton® X-114, Thesit®, Isotridecypoly(ethylene glycol ether)_n, 3-[(3-cholamidopropyl)dimethylamminio]-1-propane sulfonate (CHAPS), 3-[(3-cholamidopropyl)dimethylamminio]-2-hydroxy-1-propane sulfonate (CHAPSO), or N-dodecyl-N,N-dimethyl-3-ammonio-1-propane sulfonate.

An interaction between a test molecule and target molecule also can be detected by monitoring fluorescence energy transfer (FET) (see, e.g., Lakowicz et al., U.S. Pat. No. 5,631,169; Stavrianopoulos et al. U.S. Pat. No. 4,868,103). A fluorophore label on a first, “donor” molecule is selected such that its emitted fluorescent energy will be absorbed by a fluorescent label on a second, “acceptor” molecule, which in turn is able to fluoresce due to the absorbed energy. Alternately, the “donor” polypeptide molecule may simply utilize the natural fluorescent energy of tryptophan residues. Labels are chosen that emit different wavelengths of light, such that the “acceptor” molecule label may be differentiated from that of the “donor”. Since the efficiency of energy transfer between the labels is related to the distance separating the molecules, the spatial relationship between the molecules can be assessed. In a situation in which binding occurs between the molecules, the fluorescent emission of the “acceptor” molecule label in the assay should be maximal. An FET binding event can be conveniently measured through standard fluorometric detection means well known in the art (e.g. using a fluorimeter).

In another embodiment, determining the presence or absence of an interaction between a test molecule and a target molecule can be effected by monitoring surface plasmon resonance (see, e.g., Sjolander & Urbaniczk, Anal. Chem. 63: 2338-2345 (1991) and Szabo et al., Curr. Opin. Struct. Biol. 5: 699-705 (1995)). “Surface plasmon resonance” or “biomolecular interaction analysis (BIA)” can be utilized to detect biospecific interactions in real time, without labeling any of the interactants (e.g., BIAcore). Changes in the mass at the binding surface (indicative of a binding event) result in alterations of the refractive index of light near the surface (the optical phenomenon of surface plasmon resonance (SPR)), resulting in a detectable signal which can be used as an indication of real-time reactions between biological molecules.

In another embodiment, the target molecule or test molecules are anchored to a solid phase, facilitating the detection of target molecule/test molecule complexes and separation of the complexes from free, uncomplexed molecules. The target molecule or test molecule is immobilized to the solid support. In an embodiment, the target molecule is anchored to a solid surface, and the test molecule, which is not anchored, can be labeled, either directly or indirectly, with detectable labels discussed herein.

It may be desirable to immobilize a target molecule, an anti-target molecule antibody, and/or test molecules to facilitate separation of target molecule/test molecule complexes from uncomplexed forms, as well as to accommodate automation of the assay. The attachment between a test molecule and/or target molecule and the solid support may be covalent or non-covalent (see, e.g., U.S. Pat. No. 6,022,688 for non-covalent attachments). The solid support may be one or more surfaces of the system, such as one or more surfaces in each well of a microtiter plate, a surface of a silicon wafer, a surface of a bead (see, e.g., Lam, Nature 354: 82-84 (1991)) that is optionally linked to another solid support, or a channel in a microfluidic device, for example. Types of solid supports, linker molecules for covalent and non-covalent attachments to solid supports, and methods for immobilizing nucleic acids and other molecules to solid supports are well known (see, e.g., U.S. Pat. Nos. 6,261,776; 5,900,481; 6,133,436; and 6,022,688; and WIPO publication WO 01/18234).

In an embodiment, target molecule may be immobilized to surfaces via biotin and streptavidin. For example, biotinylated target polypeptide can be prepared from biotin-NHS (N-hydroxy-succinimide) using techniques known in the art (e.g., biotinylation kit, Pierce Chemicals, Rockford, Ill.), and immobilized in the wells of streptavidin-coated 96 well plates (Pierce Chemical). In another embodiment, a target polypeptide can be prepared as a fusion polypeptide. For example, glutathione-S-transferase/target polypeptide fusion can be adsorbed onto glutathione sepharose beads (Sigma Chemical, St. Louis, Mo.) or glutathione derivitized microtiter plates, which are then combined with a test molecule under conditions conducive to complex formation (e.g., at physiological conditions for salt and pH). Following incubation, the beads or microtiter plate wells are washed to remove any unbound components, or the matrix is immobilized in the case of beads, and complex formation is determined directly or indirectly as described above. Alternatively, the complexes can be dissociated from the matrix, and the level of target molecule binding or activity is determined using standard techniques.

In an embodiment, the non-immobilized component is added to the coated surface containing the anchored component. After the reaction is complete, unreacted components are removed (e.g., by washing) under conditions such that a significant percentage of complexes formed will remain immobilized to the solid surface. The detection of complexes anchored on the solid surface can be accomplished in a number of manners. Where the previously non-immobilized component is pre-labeled, the detection of label immobilized on the surface indicates that complexes were formed. Where the previously non-immobilized component is not pre-labeled, an indirect label can be used to detect complexes anchored on the surface, e.g., by adding a labeled antibody specific for the immobilized component, where the antibody, in turn, can be directly labeled or indirectly labeled with, e.g., a labeled anti-Ig antibody.

In another embodiment, an assay is performed utilizing antibodies that specifically bind target molecule or test molecule but do not interfere with binding of the target molecule to the test molecule. Such antibodies can be derivitized to a solid support, and unbound target molecule may be immobilized by antibody conjugation. Methods for detecting such complexes, in addition to those described above for the GST-immobilized complexes, include immunodetection of complexes using antibodies reactive with the target molecule, as well as enzyme-linked assays which rely on detecting an enzymatic activity associated with the target molecule.

Cell free assays also can be conducted in a liquid phase. In such an assay, reaction products are separated from unreacted components, by any of a number of standard techniques, including but not limited to: differential centrifugation (see, e.g., Rivas, G., and Minton, Trends Biochem Sci August; 18(8): 284-7 (1993)); chromatography (gel filtration chromatography, ion-exchange chromatography); electrophoresis (see, e.g., Ausubel et al., eds. Current Protocols in Molecular Biology, J. Wiley: New York (1999)); and immunoprecipitation (see, e.g., Ausubel et al., eds., supra). Media and chromatographic techniques are known to one skilled in the art (see, e.g., Heegaard, J Mol. Recognit. Winter; 11(1-6): 141-8 (1998); Hage & Tweed, J. Chromatogr. B Biomed. Sci. Appl. October 10; 699 (1-2): 499-525 (1997)). Further, fluorescence energy transfer may also be conveniently utilized, as described herein, to detect binding without further purification of the complex from solution.

In another embodiment, modulators of target molecule expression are identified. For example, a cell or cell free mixture is contacted with a candidate compound and the expression of target mRNA or target polypeptide is evaluated relative to the level of expression of target mRNA or target polypeptide in the absence of the candidate compound. When expression of target mRNA or target polypeptide is greater in the presence of the candidate compound than in its absence, the candidate compound is identified as an agonist of target mRNA or target polypeptide expression. Alternatively, when expression of target mRNA or target polypeptide is less (e.g., less with statistical significance) in the presence of the candidate compound than in its absence, the candidate compound is identified as an antagonist or inhibitor of target mRNA or target polypeptide expression. The level of target mRNA or target polypeptide expression can be determined by methods described herein.

In another embodiment, binding partners that interact with a target molecule are detected. The target molecules can interact with one or more cellular or extracellular macromolecules, such as polypeptides in vivo, and these interacting molecules are referred to herein as “binding partners.” Binding partners can agonize or antagonize target molecule biological activity. Also, test molecules that agonize or antagonize interactions between target molecules and binding partners can be useful as therapeutic molecules as they can up-regulate or down-regulated target molecule activity in vivo and thereby treat osteoporosis.

Binding partners of target molecules can be identified by methods known in the art. For example, binding partners may be identified by lysing cells and analyzing cell lysates by electrophoretic techniques. Alternatively, a two-hybrid assay or three-hybrid assay can be utilized (see, e.g., U.S. Pat. No. 5,283,317; Zervos et al., Cell 72:223-232 (1993); Madura et al., J. Biol. Chem. 268: 12046-12054 (1993); Bartel et al., Biotechniques 14: 920-924 (1993); Iwabuchi et al., Oncogene 8: 1693-1696 (1993); and Brent WO94/10300). A two-hybrid system is based on the modular nature of most transcription factors, which consist of separable DNA-binding and activation domains. The assay often utilizes two different DNA constructs. In one construct, a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleic acid (sometimes referred to as the “bait”) is fused to a gene encoding the DNA binding domain of a known transcription factor (e.g., GAL-4). In another construct, a DNA sequence from a library of DNA sequences that encodes a potential binding partner (sometimes referred to as the “prey”) is fused to a gene that encodes an activation domain of the known transcription factor. Sometimes, a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleic acid can be fused to the activation domain. If the “bait” and the “prey” molecules interact in vivo, the DNA-binding and activation domains of the transcription factor are brought into close proximity. This proximity allows transcription of a reporter gene (e.g., LacZ) which is operably linked to a transcriptional regulatory site responsive to the transcription factor. Expression of the reporter gene can be detected and cell colonies containing the functional transcription factor can be isolated and used to identify the potential binding partner.

In an embodiment for identifying test molecules that antagonize or agonize complex formation between target molecules and binding partners, a reaction mixture containing the target molecule and the binding partner is prepared, under conditions and for a time sufficient to allow complex formation. The reaction mixture often is provided in the presence or absence of the test molecule. The test molecule can be included initially in the reaction mixture, or can be added at a time subsequent to the addition of the target molecule and its binding partner. Control reaction mixtures are incubated without the test molecule or with a placebo. Formation of any complexes between the target molecule and the binding partner then is detected. Decreased formation of a complex in the reaction mixture containing test molecule as compared to in a control reaction mixture indicates that the molecule antagonizes target molecule/binding partner complex formation. Alternatively, increased formation of a complex in the reaction mixture containing test molecule as compared to in a control reaction mixture indicates that the molecule agonizes target molecule/binding partner complex formation. In another embodiment, complex formation of target molecule/binding partner can be compared to complex formation of mutant target molecule/binding partner (e.g., amino acid modifications in a target polypeptide). Such a comparison can be important in those cases where it is desirable to identify test molecules that modulate interactions of mutant but not non-mutated target gene products.

The assays can be conducted in a heterogeneous or homogeneous format. In heterogeneous assays, target molecule and/or the binding partner are immobilized to a solid phase, and complexes are detected on the solid phase at the end of the reaction. In homogeneous assays, the entire reaction is carried out in a liquid phase. In either approach, the order of addition of reactants can be varied to obtain different information about the molecules being tested. For example, test compounds that agonize target molecule/binding partner interactions can be identified by conducting the reaction in the presence of the test molecule in a competition format. Alternatively, test molecules that agonize preformed complexes, e.g., molecules with higher binding constants that displace one of the components from the complex, can be tested by adding the test compound to the reaction mixture after complexes have been formed.

In a heterogeneous assay embodiment, the target molecule or the binding partner is anchored onto a solid surface (e.g., a microtiter plate), while the non-anchored species is labeled, either directly or indirectly. The anchored molecule can be immobilized by non-covalent or covalent attachments. Alternatively, an immobilized antibody specific for the molecule to be anchored can be used to anchor the molecule to the solid surface. The partner of the immobilized species is exposed to the coated surface with or without the test molecule. After the reaction is complete, unreacted components are removed (e.g., by washing) such that a significant portion of any complexes formed will remain immobilized on the solid surface. Where the non-immobilized species is pre-labeled, the detection of label immobilized on the surface is indicative of complex. Where the non-immobilized species is not pre-labeled, an indirect label can be used to detect complexes anchored to the surface; e.g., by using a labeled antibody specific for the initially non-immobilized, species. Depending upon the order of addition of reaction components, test compounds that inhibit complex formation or that disrupt preformed complexes can be detected.

In another embodiment, the reaction can be conducted in a liquid phase in the presence or absence of test molecule, where the reaction products are separated from unreacted components, and the complexes are detected (e.g., using an immobilized antibody specific for one of the binding components to anchor any complexes formed in solution, and a labeled antibody specific for the other partner to detect anchored complexes). Again, depending upon the order of addition of reactants to the liquid phase, test compounds that inhibit complex or that disrupt preformed complexes can be identified.

In an alternate embodiment, a homogeneous assay can be utilized. For example, a preformed complex of the target gene product and the interactive cellular or extracellular binding partner product is prepared. One or both of the target molecule or binding partner is labeled, and the signal generated by the label(s) is quenched upon complex formation (e.g., U.S. Pat. No. 4,109,496 that utilizes this approach for immunoassays). Addition of a test molecule that competes with and displaces one of the species from the preformed complex will result in the generation of a signal above background. In this way, test substances that disrupt target molecule/binding partner complexes can be identified.

Candidate therapeutics for treating osteoporosis are identified from a group of test molecules that interact with a target molecule. Test molecules are normally ranked according to the degree with which they modulate (e.g. agonize or antagonize) a function associated with the target molecule (e.g., DNA replication and/or processing, RNA transcription and/or processing, polypeptide production and/or processing, and/or biological function/activity), and then top ranking modulators are selected. Also, pharmacogenomic information described herein can determine the rank of a modulator. The top 10% of ranked test molecules often are selected for further testing as candidate therapeutics, and sometimes the top 15%, 20%, or 25% of ranked test molecules are selected for further testing as candidate therapeutics. Candidate therapeutics typically are formulated for administration to a subject.

Therapeutic Formulations

Formulations and pharmaceutical compositions typically include in combination with a pharmaceutically acceptable carrier one or more target molecule modulators. The modulator often is a test molecule identified as having an interaction with a target molecule by a screening method described above. The modulator may be a compound, an antisense nucleic acid, a ribozyme, an antibody, or a binding partner. Also, formulations may comprise a target polypeptide or fragment thereof in combination with a pharmaceutically acceptable carrier.

As used herein, the term “pharmaceutically acceptable carrier” includes solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, and the like, compatible with pharmaceutical administration. Supplementary active compounds can also be incorporated into the compositions. Pharmaceutical compositions can be included in a container, pack, or dispenser together with instructions for administration.

A pharmaceutical composition typically is formulated to be compatible with its intended route of administration. Examples of routes of administration include parenteral, e.g., intravenous, intradermal, subcutaneous, oral (e.g., inhalation), transdermal (topical), transmucosal, and rectal administration. Solutions or suspensions used for parenteral, intradermal, or subcutaneous application can include the following components: a sterile diluent such as water for injection, saline solution, fixed oils, polyethylene glycols, glycerin, propylene glycol or other synthetic solvents; antibacterial agents such as benzyl alcohol or methyl parabens; antioxidants such as ascorbic acid or sodium bisulfite; chelating agents such as ethylenediaminetetraacetic acid; buffers such as acetates, citrates or phosphates and agents for the adjustment of tonicity such as sodium chloride or dextrose. pH can be adjusted with acids or bases, such as hydrochloric acid or sodium hydroxide. The parenteral preparation can be enclosed in ampoules, disposable syringes or multiple dose vials made of glass or plastic.

Oral compositions generally include an inert diluent or an edible carrier. For the purpose of oral therapeutic administration, the active compound can be incorporated with excipients and used in the form of tablets, troches, or capsules, e.g., gelatin capsules. Oral compositions can also be prepared using a fluid carrier for use as a mouthwash. Pharmaceutically compatible binding agents, and/or adjuvant materials can be included as part of the composition. The tablets, pills, capsules, troches and the like can contain any of the following ingredients, or compounds of a similar nature: a binder such as microcrystalline cellulose, gum tragacanth or gelatin; an excipient such as starch or lactose, a disintegrating agent such as alginic acid, Primogel, or corn starch; a lubricant such as magnesium stearate or Sterotes; a glidant such as colloidal silicon dioxide; a sweetening agent such as sucrose or saccharin; or a flavoring agent such as peppermint, methyl salicylate, or orange flavoring.

Pharmaceutical compositions suitable for injectable use include sterile aqueous solutions (where water soluble) or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersion. For intravenous administration, suitable carriers include physiological saline, bacteriostatic water, Cremophor EL™ (BASF, Parsippany, N.J.) or phosphate buffered saline (PBS). In all cases, the composition must be sterile and should be fluid to the extent that easy syringability exists. It should be stable under the conditions of manufacture and storage and must be preserved against the contaminating action of microorganisms such as bacteria and fungi. The carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (for example, glycerol, propylene glycol, and liquid polyethylene glycol, and the like), and suitable mixtures thereof. The proper fluidity can be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersion and by the use of surfactants. Prevention of the action of microorganisms can be achieved by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, ascorbic acid, thimerosal, and the like. In many cases, it will be preferable to include isotonic agents, for example, sugars, polyalcohols such as mannitol, sorbitol, sodium chloride in the composition. Prolonged absorption of the injectable compositions can be brought about by including in the composition an agent which delays absorption, for example, aluminum monostearate and gelatin.

Sterile injectable solutions can be prepared by incorporating the active compound in the required amount in an appropriate solvent with one or a combination of ingredients enumerated above, as required, followed by filtered sterilization. Generally, dispersions are prepared by incorporating the active compound into a sterile vehicle which contains a basic dispersion medium and the required other ingredients from those enumerated above. In the case of sterile powders for the preparation of sterile injectable solutions, the preferred methods of preparation are vacuum drying and freeze-drying which yields a powder of the active ingredient plus any additional desired ingredient from a previously sterile-filtered solution thereof.

For administration by inhalation, the compounds are delivered in the form of an aerosol spray from pressured container or dispenser which contains a suitable propellant, e.g., a gas such as carbon dioxide, or a nebulizer.

Systemic administration can also be by transmucosal or transdermal means. For transmucosal or transdermal administration, penetrants appropriate to the barrier to be permeated are used in the formulation. Such penetrants are generally known in the art, and include, for example, for transmucosal administration, detergents, bile salts, and fusidic acid derivatives. Transmucosal administration can be accomplished through the use of nasal sprays or suppositories. For transdermal administration, the active compounds are formulated into ointments, salves, gels, or creams as generally known in the art. Molecules can also be prepared in the form of suppositories (e.g., with conventional suppository bases such as cocoa butter and other glycerides) or retention enemas for rectal delivery.

In one embodiment, active molecules are prepared with carriers that will protect the compound against rapid elimination from the body, such as a controlled release formulation, including implants and microencapsulated delivery systems. Biodegradable, biocompatible polymers can be used, such as ethylene vinyl acetate, polyanhydrides, polyglycolic acid, collagen, polyorthoesters, and polylactic acid. Methods for preparation of such formulations will be apparent to those skilled in the art. Materials can also be obtained commercially from Alza Corporation and Nova Pharmaceuticals, Inc. Liposomal suspensions (including liposomes targeted to infected cells with monoclonal antibodies to viral antigens) can also be used as pharmaceutically acceptable carriers. These can be prepared according to methods known to those skilled in the art, for example, as described in U.S. Pat. No. 4,522,811.

It is advantageous to formulate oral or parenteral compositions in dosage unit form for ease of administration and uniformity of dosage. Dosage unit form as used herein refers to physically discrete units suited as unitary dosages for the subject to be treated; each unit containing a predetermined quantity of active compound calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier.

Toxicity and therapeutic efficacy of such compounds can be determined by standard pharmaceutical procedures in cell cultures or experimental animals, e.g., for determining the LD₅₀ (the dose lethal to 50% of the population) and the ED₅₀ (the dose therapeutically effective in 50% of the population). The dose ratio between toxic and therapeutic effects is the therapeutic index and it can be expressed as the ratio LD₅₀/ED₅₀. Molecules which exhibit high therapeutic indices are preferred. While molecules that exhibit toxic side effects may be used, care should be taken to design a delivery system that targets such compounds to the site of affected tissue in order to minimize potential damage to uninfected cells and, thereby, reduce side effects.

The data obtained from the cell culture assays and animal studies can be used in formulating a range of dosage for use in humans. The dosage of such molecules lies preferably within a range of circulating concentrations that include the ED₅₀ with little or no toxicity. The dosage may vary within this range depending upon the dosage form employed and the route of administration utilized. For any molecules used in the methods described herein, the therapeutically effective dose can be estimated initially from cell culture assays. A dose may be formulated in animal models to achieve a circulating plasma concentration range that includes the IC₅₀ (i.e., the concentration of the test compound which achieves a half-maximal inhibition of symptoms) as determined in cell culture. Such information can be used to more accurately determine useful doses in humans. Levels in plasma may be measured, for example, by high performance liquid chromatography.

As defined herein, a therapeutically effective amount of protein or polypeptide (i.e., an effective dosage) ranges from about 0.001 to 30 mg/kg body weight, sometimes about 0.01 to 25 mg/kg body weight, often about 0.1 to 20 mg/kg body weight, and more often about 1 to 10 mg/kg, 2 to 9 mg/kg, 3 to 8 mg/kg, 4 to 7 mg/kg, or 5 to 6 mg/kg body weight. The protein or polypeptide can be administered one time per week for between about 1 to 10 weeks, sometimes between 2 to 8 weeks, often between about 3 to 7 weeks, and more often for about 4, 5, or 6 weeks. The skilled artisan will appreciate that certain factors may influence the dosage and timing required to effectively treat a subject, including but not limited to the severity of the disease or disorder, previous treatments, the general health and/or age of the subject, and other diseases present. Moreover, treatment of a subject with a therapeutically effective amount of a protein, polypeptide, or antibody can include a single treatment or, preferably, can include a series of treatments.

With regard to polypeptide formulations, featured herein is a method for treating osteoporosis in a subject, which comprises contacting one or more cells in the subject with a first polypeptide, where the subject comprises a second polypeptide having one or more polymorphic variations associated with low BMD, and where the first polypeptide comprises fewer polymorphic variations associated with low BMD than the second polypeptide. The first and second polypeptides are encoded by a nucleic acid which comprises a nucleotide sequence in SEQ ID NO's:1-10; a nucleotide sequence which encodes a polypeptide consisting of an amino acid sequence encoded by a nucleotide sequence referenced in SEQ ID NO's:1-10; a nucleotide sequence which encodes a polypeptide that is 90% or more identical to an amino acid sequence encoded by a nucleotide sequence of SEQ ID NO's:1-10 and a nucleotide sequence 90% or more identical to a nucleotide sequence in SEQ ID NO's:1-10. The subject often is a human.

For antibodies, a dosage of 0.1 mg/kg of body weight (generally 10 mg/kg to 20 mg/kg) is often utilized. If the antibody is to act in the brain, a dosage of 50 mg/kg to 100 mg/kg is often appropriate. Generally, partially human antibodies and fully human antibodies have a longer half-life within the human body than other antibodies. Accordingly, lower dosages and less frequent administration is often possible. Modifications such as lipidation can be used to stabilize antibodies and to enhance uptake and tissue penetration (e.g., into the brain). A method for lipidation of antibodies is described by Cruikshank et al., J. Acquired Immune Deficiency Syndromes and Human Retrovirology 14:193 (1997).

Antibody conjugates can be used for modifying a given biological response, the drug moiety is not to be construed as limited to classical chemical therapeutic agents. For example, the drug moiety may be a protein or polypeptide possessing a desired biological activity. Such proteins may include, for example, a toxin such as abrin, ricin A, pseudomonas exotoxin, or diphtheria toxin; a polypeptide such as tumor necrosis factor, .alpha.-interferon, .beta.-interferon, nerve growth factor, platelet derived growth factor, tissue plasminogen activator; or, biological response modifiers such as, for example, lymphokines, interleukin-1 (“IL-1”), interleukin-2 (“IL-2”), interleukin-6 (“IL-6”), granulocyte macrophage colony stimulating factor (“GM-CSF”), granulocyte colony stimulating factor (“G-CSF”), or other growth factors. Alternatively, an antibody can be conjugated to a second antibody to form an antibody heteroconjugate as described by Segal in U.S. Pat. No. 4,676,980.

For compounds, exemplary doses include milligram or microgram amounts of the compound per kilogram of subject or sample weight, for example, about 1 microgram per kilogram to about 500 milligrams per kilogram, about 100 micrograms per kilogram to about 5 milligrams per kilogram, or about 1 microgram per kilogram to about 50 micrograms per kilogram. It is understood that appropriate doses of a small molecule depend upon the potency of the small molecule with respect to the expression or activity to be modulated. When one or more of these small molecules is to be administered to an animal (e.g., a human) in order to modulate expression or activity of a polypeptide or nucleic acid described herein, a physician, veterinarian, or researcher may, for example, prescribe a relatively low dose at first, subsequently increasing the dose until an appropriate response is obtained. In addition, it is understood that the specific dose level for any particular animal subject will depend upon a variety of factors including the activity of the specific compound employed, the age, body weight, general health, gender, and diet of the subject, the time of administration, the route of administration, the rate of excretion, any drug combination, and the degree of expression or activity to be modulated.

With regard to nucleic acid formulations, gene therapy vectors can be delivered to a subject by, for example, intravenous injection, local administration (see, e.g., U.S. Pat. No. 5,328,470) or by stereotactic injection (see e.g., Chen et al., (1994) Proc. Natl. Acad. Sci. USA 91:3054-3057). Pharmaceutical preparations of gene therapy vectors can include a gene therapy vector in an acceptable diluent, or can comprise a slow release matrix in which the gene delivery vehicle is imbedded. Alternatively, where the complete gene delivery vector can be produced intact from recombinant cells (e.g., retroviral vectors) the pharmaceutical preparation can include one or more cells which produce the gene delivery system. Examples of gene delivery vectors are described herein.

Therapeutic Methods

A therapeutic formulation described above can be administered to a subject in need of a therapeutic for inducing a desired biological response. Therapeutic formulations can be administered by any of the paths described herein. With regard to both prophylactic and therapeutic methods of treatment, such treatments may be specifically tailored or modified, based on knowledge obtained from pharmacogenomic analyses described herein.

As used herein, the term “treatment” is defined as the application or administration of a therapeutic formulation to a subject, or application or administration of a therapeutic agent to an isolated tissue or cell line from a subject with the purpose to cure, heal, alleviate, relieve, alter, remedy, ameliorate, improve or affect osteoporosis, symptoms of osteoporosis or a predisposition towards osteoporosis. A therapeutic formulation includes, but is not limited to, small molecules, peptides, antibodies, ribozymes and antisense oligonucleotides. Administration of a therapeutic formulation can occur prior to the manifestation of symptoms characteristic of low BMD, such that osteoporosis is prevented or delayed in its progression. The appropriate therapeutic composition can be determined based on screening assays described herein.

As discussed, successful treatment of osteoporosis can be brought about by techniques that serve to agonize target molecule expression or function, or alternatively, antagonize target molecule expression or function. These techniques include administration of modulators that include, but are not limited to, small organic or inorganic molecules; antibodies (including, for example, polyclonal, monoclonal, humanized, anti-idiotypic, chimeric or single chain antibodies, and Fab, F(ab′)₂ and Fab expression library fragments, scFV molecules, and epitope-binding fragments thereof); and peptides, phosphopeptides, or polypeptides.

Further, antisense and ribozyme molecules that inhibit expression of the target gene can also be used to reduce the level of target gene expression, thus effectively reducing the level of target gene activity. Still further, triple helix molecules can be utilized in reducing the level of target gene activity. Antisense, ribozyme and triple helix molecules are discussed above. It is possible that the use of antisense, ribozyme, and/or triple helix molecules to reduce or inhibit mutant gene expression can also reduce or inhibit the transcription (triple helix) and/or translation (antisense, ribozyme) of mRNA produced by normal target gene alleles, such that the concentration of normal target gene product present can be lower than is necessary for a normal phenotype. In such cases, nucleic acid molecules that encode and express target gene polypeptides exhibiting normal target gene activity can be introduced into cells via gene therapy method. Alternatively, in instances in that the target gene encodes an extracellular polypeptide, it can be preferable to co-administer normal target gene polypeptide into the cell or tissue in order to maintain the requisite level of cellular or tissue target gene activity.

Another method by which nucleic acid molecules may be utilized in treating or preventing osteoporosis is use of aptamer molecules specific for target molecules. Aptamers are nucleic acid molecules having a tertiary structure which permits them to specifically bind to ligands (see, e.g., Osborne, et al., Curr. Opin. Chem. Biol. 1(1): 5-9 (1997); and Patel, D. J., Curr. Opin. Chem. Biol. June; 1(1): 32-46 (1997)).

Yet another method of utilizing nucleic acid molecules for osteoporosis treatment is gene therapy, which can also be referred to as allele therapy. Provided herein is a gene therapy method for treating osteoporosis in a subject, which comprises contacting one or more cells in the subject or from the subject with a nucleic acid having a first nucleotide sequence. Genomic DNA in the subject comprises a second nucleotide sequence having one or more polymorphic variations associated with low BMD (e.g., the second nucleic acid has a nucleotide sequence in SEQ ID NO's:1-10). The first and second nucleotide sequences typically are substantially identical to one another, and the first nucleotide sequence comprises fewer polymorphic variations associated with low BMD than the second nucleotide sequence. The first nucleotide sequence may comprise a gene sequence that encodes a full-length polypeptide or a fragment thereof. The subject is often a human. Allele therapy methods often are utilized in conjunction with a method of first determining whether a subject has genomic DNA that includes polymorphic variants associated with low BMD.

In another allele therapy embodiment, provided herein is a method which comprises contacting one or more cells in the subject or from the subject with a polypeptide encoded by a nucleic acid having a first nucleotide sequence. Genomic DNA in the subject comprises a second nucleotide sequence having one or more polymorphic variations associated with low BMD (e.g., the second nucleic acid has a nucleotide sequence in SEQ ID NO's:1-10). The first and second nucleotide sequences typically are substantially identical to one another, and the first nucleotide sequence comprises fewer polymorphic variations associated with low BMD than the second nucleotide sequence. The first nucleotide sequence may comprise a gene sequence that encodes a full-length polypeptide or a fragment thereof. The subject is often a human.

For antibody-based therapies, antibodies can be generated that are both specific for target molecules and that reduce target molecule activity. Such antibodies may be administered in instances where antagonizing a target molecule function is appropriate for the treatment of osteoporosis.

In circumstances where stimulating antibody production in an animal or a human subject by injection with a target molecule is harmful to the subject, it is possible to generate an immune response against the target molecule by use of anti-idiotypic antibodies (see, e.g., Herlyn, Ann. Med.; 31(1): 66-78 (1999); and Bhattacharya-Chatterjee & Foon, Cancer Treat. Res.; 94: 51-68 (1998)). Introducing an anti-idiotypic antibody to a mammal or human subject often stimulates production of anti-anti-idiotypic antibodies, which typically are specific to the target molecule. Vaccines directed to osteoporosis also may be generated in this fashion.

In instances where the target molecule is intracellular and whole antibodies are used, internalizing antibodies may be preferred. Lipofectin or liposomes can be used to deliver the antibody or a fragment of the Fab region that binds to the target antigen into cells. Where fragments of the antibody are used, the smallest inhibitory fragment that binds to the target antigen is preferred. For example, peptides having an amino acid sequence corresponding to the Fv region of the antibody can be used. Alternatively, single chain neutralizing antibodies that bind to intracellular target antigens can also be administered. Such single chain antibodies can be administered, for example, by expressing nucleotide sequences encoding single-chain antibodies within the target cell population (see, e.g., Marasco et al., Proc. Natl. Acad. Sci. USA 90: 7889-7893 (1993)).

Modulators can be administered to a patient at therapeutically effective doses to treat osteoporosis. A therapeutically effective dose refers to an amount of the modulator sufficient to result in amelioration of symptoms of osteoporosis. Toxicity and therapeutic efficacy of modulators can be determined by standard pharmaceutical procedures in cell cultures or experimental animals, e.g., for determining the LD₅₀ (the dose lethal to 50% of the population) and the ED₅₀ (the dose therapeutically effective in 50% of the population). The dose ratio between toxic and therapeutic effects is the therapeutic index and it can be expressed as the ratio LD₅₀/ED₅₀. Modulators that exhibit large therapeutic indices are preferred. While modulators that exhibit toxic side effects can be used, care should be taken to design a delivery system that targets such molecules to the site of affected tissue in order to minimize potential damage to uninfected cells, thereby reducing side effects.

Data obtained from cell culture assays and animal studies can be used in formulating a range of dosages for use in humans. The dosage of such compounds lies preferably within a range of circulating concentrations that include the ED₅₀ with little or no toxicity. The dosage can vary within this range depending upon the dosage form employed and the route of administration utilized. For any compound used in the methods described herein, the therapeutically effective dose can be estimated initially from cell culture assays. A dose can be formulated in animal models to achieve a circulating plasma concentration range that includes the IC₅₀ (i.e., the concentration of the test compound that achieves a half-maximal inhibition of symptoms) as determined in cell culture. Such information can be used to more accurately determine useful doses in humans. Levels in plasma can be measured, for example, by high performance liquid chromatography.

Another example of effective dose determination for an individual is the ability to directly assay levels of “free” and “bound” compound in the serum of the test subject. Such assays may utilize antibody mimics and/or “biosensors” that have been created through molecular imprinting techniques. Molecules that modulate target molecule activity are used as a template, or “imprinting molecule”, to spatially organize polymerizable monomers prior to their polymerization with catalytic reagents. The subsequent removal of the imprinted molecule leaves a polymer matrix which contains a repeated “negative image” of the compound and is able to selectively rebind the molecule under biological assay conditions. A detailed review of this technique can be seen in Ansell et al., Current Opinion in Biotechnology 7: 89-94 (1996) and in Shea, Trends in Polymer Science 2: 166-173 (1994). Such “imprinted” affinity matrixes are amenable to ligand-binding assays, whereby the immobilized monoclonal antibody component is replaced by an appropriately imprinted matrix. An example of the use of such matrixes in this way can be seen in Vlatakis, et al., Nature 361: 645-647 (1993). Through the use of isotope-labeling, the “free” concentration of compound which modulates target molecule expression or activity readily can be monitored and used in calculations of IC₅₀. Such “imprinted” affinity matrixes can also be designed to include fluorescent groups whose photon-emitting properties measurably change upon local and selective binding of target compound. These changes readily can be assayed in real time using appropriate fiberoptic devices, in turn allowing the dose in a test subject to be quickly optimized based on its individual IC₅₀. An example of such a “biosensor” is discussed in Kriz et al., Analytical Chemistry 67: 2142-2144 (1995).

The examples set forth below are intended to illustrate but not limit the invention.

EXAMPLES

In the following studies a group of subjects were selected according to specific parameters relating to low BMD. Nucleic acid samples obtained from individuals in the study group were subjected to genetic analysis, which identified associations between low BMD and certain polymorphic variants in CETP, PROL4, GRID2, PDE4D and GPX3 (herein referred to as “target genes”, “target nucleotides”, “target polypeptides” or simply “targets”). Methods are described for producing CETP, PROL4, GRID2, PDE4D and GPX3 polypeptides and polypeptide variants in vitro or in vivo. CETP, PROL4, GRID2, PDE4D and GPX3 nucleic acids or polypeptides and variants thereof are utilized for screening test molecules for those that interact with CETP, PROL4, GRID2, PDE4D and GPX3 molecules. Test molecules identified as being interactors with target polypeptides can be screened further as osteoporosis therapeutics.

Example 1

Samples and Pooling Strategies

Sample Selection

Blood samples were collected from individuals with low BMD, which were referred to as case samples. Also, blood samples were collected from individuals with high BMD (i.e., not diagnosed with osteoporosis or any form of osteoporosis-related disease); these samples served as gender and age-matched controls. All of the samples were of Caucasian (English) descent A database was created that listed all phenotypic trait information gathered from individuals for each case and control sample. Genomic DNA was extracted from each of the blood samples for genetic analyses.

DNA Extraction from Blood Samples

Six to ten milliliters of whole blood was transferred to a 50 ml tube containing 27 ml of red cell lysis solution (RCL). The tube was inverted until the contents were mixed. Each tube was incubated for 10 minutes at room temperature and inverted once during the incubation. The tubes were then centrifuged for 20 minutes at 3000×g and the supernatant was carefully poured off. 100-200 μl of residual liquid was left in the tube and was pipetted repeatedly to resuspend the pellet in the residual supernatant White cell lysis solution (WCL) was added to the tube and pipetted repeatedly until completely mixed. While no incubation was normally required, the solution was incubated at 37° C. or room temperature if cell clumps were visible after mixing until the solution was homogeneous. 2 ml of protein precipitation was added to the cell lysate. The mixtures were vortexed vigorously at high speed for 20 sec to mix the protein precipitation solution uniformly with the cell lysate, and then centrifuged for 10 minutes at 3000×g. The supernatant containing the DNA was then poured into a clean 15 ml tube, which contained 7 ml of 100% isopropanol. The samples were mixed by inverting the tubes gently until white threads of DNA were visible. Samples were centrifuged for 3 minutes at 2000×g and the DNA was visible as a small white pellet. The supernatant was decanted and 5 ml of 70% ethanol was added to each tube. Each tube was inverted several times to wash the DNA pellet, and then centrifuged for 1 minute at 2000×g. The ethanol was decanted and each tube was drained on clean absorbent paper. The DNA was dried in the tube by inversion for 10 minutes, and then 1000 μl of 1×TE was added. The size of each sample was estimated, and less TE buffer was added during the following DNA hydration step if the sample was smaller. The DNA was allowed to rehydrate overnight at room temperature, and DNA samples were stored at 2-8° C.

DNA was quantified by placing samples on a hematology mixer for at least 1 hour. DNA was serially diluted (typically 1:80, 1:160, 1:320, and 1:640 dilutions) so that it would be within the measurable range of standards. 125 μl of diluted DNA was transferred to a clear U-bottom microtitre plate, and 125 μl of 1×TE buffer was transferred into each well using a multichannel pipette. The DNA and 1×TE were mixed by repeated pipetting at least 15 times, and then the plates were sealed. 50 μl of diluted DNA was added to wells A5-H12 of a black flat bottom microtitre plate. Standards were inverted six times to mix them, and then 50 μl of 1×TE buffer was pipetted into well A1, 1000 ng/ml of standard was pipetted into well A2, 500 ng/ml of standard was pipetted into well A3, and 250 ng/ml of standard was pipetted into well A4. PicoGreen (Molecular Probes, Eugene, Oreg.) was thawed and freshly diluted 1:200 according to the number of plates that were being measured. PicoGreen was vortexed and then 50 μl was pipetted into all wells of the black plate with the diluted DNA. DNA and PicoGreen were mixed by pipetting repeatedly at least 10 times with the multichannel pipette. The plate was placed into a Fluoroskan Ascent Machine (microplate fluorometer produced by Labsystems) and the samples were allowed to incubate for 3 minutes before the machine was run using filter pairs 485 nm excitation and 538 nm emission wavelengths. Samples having measured DNA concentrations of greater than 450 ng/l were re-measured for conformation. Samples having measured DNA concentrations of 20 ng/μl or less were re-measured for confirmation.

Pooling Strategies

Samples were placed into one of two groups based on BMD levels. The two groups were made up of individuals with low BMD levels and individuals with high BMD levels. A select set of samples from each group were utilized to generate pools, and one pool was created for each group. Each individual sample in a pool was represented by an equal amount of genomic DNA. For example, where 25 ng of genomic DNA was utilized in each PCR reaction and there were 200 individuals in each pool, each individual would provide 125 pg of genomic DNA. Inclusion or exclusion of samples for a pool was based upon the following criteria: the sample was derived from an individual of Caucasian paternal and maternal descent; the database included relevant phenotype information for the individual; case samples were derived from individuals with low BMD; control samples were derived from individuals with normal or high BMD and no history of osteoporosis or osteoporosis-related diseases; and sufficient genomic DNA was extracted from each blood sample for all allelotyping and genotyping reactions performed during the study. Phenotype information included pre- or post-menopausal, familial predisposition, country or origin of mother and father, diagnosis with osteoporosis (date of primary diagnosis, age of individual as of primary diagnosis, osteoporosis-related fracture), biochemical measurements of markers of bone resorption (bone-specific alkaline Phosphatase, Urinary C-telopeptide of type I collagen, serum osteocalcin), current medication status (thyroid medication, hormone replacement therapy, steroid usage, bisphosphonates and cytotoxic agents for rheumatic diseases). Samples that met the inclusion criteria and did not meet the exclusion criteria were added to appropriate pools based on gender and disease status.

The selection process yielded the pools set forth in Table 2, which were used in the studies that follow. The average (mean) T-score in the High group is 1.56, and in the Low group −2.1 which means that on average individuals in the high BMD pool are 1.56 standard deviations above the average BMD in young females, whereas individuals in the low BMD pool are on average 2.1 standard deviations below the normal young value.

	TABLE 2
	Female High BMD	Female Low BMD
Pool size	321	319
(Number)
Mean T-score	1.56	−2.10
(BMD leveles adjusted for
age and BMI)
Mean Age	52.9	52.4
(ex: years)

Example 2

Association of Polymorphic Variants with Low BMD

A whole-genome screen was performed to identify particular SNPs associated with low BMD. As described in Example 1, two groups of samples were utilized, which included samples from female individuals having low BMD (osteoporosis cases), and samples from female individuals having high BMD levels (controls). The initial screen of each pool was performed in an allelotyping study, in which certain samples in each group were pooled. By pooling DNA from each group, an allele frequency for each SNP in each group was calculated. These allele frequencies were then compared to one another. Particular SNPs were considered as being associated with low BMD when allele frequency differences calculated between case and control pools were statistically significant. SNP disease association results obtained from the allelotyping study were then validated by genotyping each associated SNP across all samples from each pool. The results of the genotyping were then analyzed, allele frequencies for each group were calculated from the individual genotyping results, and a p-value was calculated to determine whether the case and control groups had statistically significantly differences in allele frequencies for a particular SNP. When the genotyping results agreed with the original allelotyping results, the SNP disease association was considered validated at the genetic level.

SNP Panel Used for Genetic Analyses

A whole-genome SNP screen began with an initial screen of approximately 25,000 SNPs over each set of disease and control samples using a pooling approach. The pools studied in the screen are described in Example 1. The SNPs analyzed in this study were part of a set of 25,488 SNPs confirmed as being statistically polymorphic as each is characterized as having a minor allele frequency of greater than 10%. The SNPs in the set reside in genes or in close proximity to genes, and many reside in gene exons. Specifically, SNPs in the set are located in exons, introns, and within 5,000 base-pairs upstream of a transcription start site of a gene. In addition, SNPs were selected according to the following criteria: they are located in ESTs; they are located in Locuslink or Ensemb1 genes; and they are located in Genomatix promoter predictions. SNPs in the set were also selected on the basis of even spacing across the genome, as depicted in Table 3. An additional 3088 SNPs were included with these 25,488 SNPs and these additional SNPs had been chosen on the basis of gene location, with preference to non-synonymous coding SNPs located in disease candidate genes.

TABLE 3
General Statistics	Spacing Statistics
Total # of SNPs	25,488	Median	37,058 bp
# of Exonic SNPs	>4,335 (17%)	Minimum*	1,000 bp
# SNPs with	20,776 (81%)	Maximum*	3,000,000 bp
refSNP ID
Gene Coverage	>10,000	Mean	122,412 bp
Chromosome	All	Std Deviation	373,325 pb
Coverage
*Excludes outliers

Allelotyping and Genotyping Results

The genetic studies summarized above and described in more detail below identified allelic variants associated with low BMD. The allelic variants identified from the SNP panel described in Table 3 are summarized below in Table 4.

TABLE 4
										Low
			Position							BMD
SNP		Chromosome	in SEQ	Contig	Contig	Sequence		Sequence	Allelic	Assoc.
Reference	Chromosome	Position	ID Nos	Identification	Position	Identification	Locus	Position	Variability	Allele
rs1801706	16q21	57068823	50109	NT_010498	10631861	NM_000078	CETP	UTR	AG	G
rs1047699	12p13	11143886	49075	NT_009714	3758682	NM_007244	PROL4	Exon:	CT	C
								R120Q
rs1948017	4q22	94636365	49110	NT_016354	18829520	NM_001510	GRID2	Intron	CT	C
rs1498608	5q12	59895580	49652	NT_006713	8937426	NM_006203	PDE4D	Intron	AT	T
rs869975	5q23	150475233	50082	NT_029289	11569308	NM_002084	GPX3	Intron	AG	G

Table 4 includes information pertaining to the incident polymorphic variant associated with low BMD identified herein. Public information pertaining to the polymorphism and the genomic sequence that includes the polymorphism are indicated. The genomic sequences identified in Table 4 may be accessed at the http address www.ncbi.nih.gov/entrez/query.fcgi, for example, by using the publicly available SNP reference number (e.g., rs1801706). The chromosome position refers to the position of the SNP within NCBI's Genome Build 34, which may be accessed at the following http address: www.ncbi.nlm.nih.gov/mapview/map_search.cgi?chr=hum_chr.inf&query=. The “Contig Position” provided in Table 4 corresponds to a nucleotide position set forth in the contig sequence, and designates the polymorphic site corresponding to the SNP reference number. The sequence containing the polymorphisms also may be referenced by the “Sequence Identification” set forth in Table 4. The “Sequence Identification” corresponds to cDNA sequence that encodes associated target polypeptides (e.g., CETP) of the invention. The position of the SNP within the cDNA sequence is provided in the “Sequence Position” column of Table 4. Also, the allelic variation at the polymorphic site and the allelic variant identified as associated with low BMD is specified in Table 4. All nucleotide sequences referenced and accessed by the parameters set forth in Table 4 are incorporated herein by reference.

Assay for Verifying, Allelotyping, and Genotyping SNPs

A MassARRAY® system (Sequenom, Inc.) was utilized to perform SNP genotyping in a high-throughput fashion. This genotyping platform was complemented by a homogeneous, single-tube assay method (hME™ or homogeneous MassEXTEND™ (Sequenom, Inc.)) in which two genotyping primers anneal to and amplify a genomic target surrounding a polymorphic site of interest. A third primer (the MassEXTEND™ primer), which is complementary to the amplified target up to but not including the polymorphism, was then enzymatically extended one or a few bases through the polymorphic site and then terminated.

For each polymorphism, SpectroDESIGNER™ software (Sequenom, Inc.) was used to generate a set of PCR primers and a MassEXTEND™ primer was used to genotype the polymorphism. Table 5 shows PCR primers and Table 6 shows extension primers used for analyzing polymorphisms. The initial PCR amplification reaction was performed in a 5 A1 total volume containing 1×PCR buffer with 1.5 mM MgCl₂ (Qiagen), 200 μM each of dATP, dGTP, dCTP, dTTP (Gibco-BRL), 2.5 ng of genomic DNA, 0.1 units of HotStar DNA polymerase (Qiagen), and 200 nM each of forward and reverse PCR primers specific for the polymorphic region of interest.

TABLE 5
PCR Primers
Reference	Forward	Reverse
SNP ID	PCR primer	PCR primer
rs1801706	ACGTTGGATGTTGTAGCAG	ACGTTGGATGTCCATCTCCG
	AAGGCAAGCAC	TACTCCTAAC
rs1047699	GATTACCAGAGTGGTTGCT	CCTGCAGGAAGCATCATCAT
	C
rs1948017	GTTTAACAGCAACCATTGA	CCCCAAAGGTATGTTAAGAG
	GG
rs1498608	GAATCCCTGTTCATTCCTT	ATACCTAGGTATAACCTCGG
	G
rs869975	AACTCACTGGTGATCCTGC	TGTCTCATCCACACCACTCC
	G

Samples were incubated at 95° C. for 15 minutes, followed by 45 cycles of 95° C. for 20 seconds, 56° C. for 30 seconds, and 72° C. for 1 minute, finishing with a 3 minute final extension at 720° C. Following amplification, shrimp alkaline phosphatase (SAP) (0.3 units in a 2 μl volume) (Amersham Pharmacia) was added to each reaction (total reaction volume was 7 μl) to remove any residual dNTPs that were not consumed in the PCR step. Samples were incubated for 20 minutes at 37° C., followed by 5 minutes at 85° C. to denature the SAP.

Once the SAP reaction was complete, a primer extension reaction was initiated by adding a polymorphism-specific MassEXTEND™ primer cocktail to each sample. Each MassEXTEND™ cocktail included a specific combination of dideoxynucleotides (ddNTPs) and deoxynucleotides (dNTPs) used to distinguish polymorphic alleles from one another. In Table 6, ddNTPs are shown and the fourth nucleotide not shown is the dNTP.

TABLE 6
Extend Primers
	Reference	Extend	Term
	SNP ID	Probe	Mix
	rs1801708	CCTGGTGTCTCCTCCAGC	ACT
	rs1047699	TGTCTTGCTGGTCTGTCCCTC	ACG
	rs1948017	CAGCAACCATTGAGGGTGAAT	ACG
	rs1498608	CCCTAAAAACTGTTCCAGGTA	CGT
	rs869975	GGGCCTCAGTAGTTCCAGC	ACT

The MassEXTEND™ reaction was performed in a total volume of 9 μl, with the addition of 1× ThermoSequenase buffer, 0.576 units of ThermoSequenase (Amersham Pharmacia), 600 nM MassEXTEND™ primer, 2 mM of ddATP and/or ddCTP and/or ddGTP and/or ddTTP, and 2 mM of dATP or dCTP or dGTP or dTTP. The deoxy nucleotide (dNTP) used in the assay normally was complementary to the nucleotide at the polymorphic site in the amplicon. Samples were incubated at 94° C. for 2 minutes, followed by 55 cycles of 5 seconds at 94° C., 5 seconds at 52° C., and 5 seconds at 72° C.

Following incubation, samples were desalted by adding 16 μl of water (total reaction volume was 25 μl), 3 mg of SpectroCLEAN™ sample cleaning beads (Sequenom, Inc.) and allowed to incubate for 3 minutes with rotation. Samples were then robotically dispensed using a piezoelectric dispensing device (SpectroJET™ (Sequenom, Inc.)) onto either 96-spot or 384-spot silicon chips containing a matrix that crystallized each sample (SpectroCHIP® (Sequenom, Inc.)). Subsequently, MALDI-TOF mass spectrometry (Biflex and Autoflex MALDI-TOF mass spectrometers (Bruker Daltonics) can be used) and SpectroTYPER RT™ software (Sequenom, Inc.) were used to analyze and interpret the SNP genotype for each sample.

Genetic Analysis

Variations identified in the target genes are provided in their respective genomic sequences (see SEQ ID NOs:1-4) Minor allelic frequencies for these polymorphisms was verified as being 10% or greater by determining the allelic frequencies using the extension assay described above in a group of samples isolated from 92 individuals originating from the state of Utah in the United States, Venezuela and France (Coriell cell repositories).

Genotyping results for the allelic variant set forth in Table 4 are shown for female pools in Table 7. In Table 7, “F case” and “F control” refer to female case (low BMD) and female control (high BMD) groups, and “AF” refers to allele frequency.

TABLE 7
Female Genotype Results
					Low BMD
SNP	F AF case	F AF control		Odds	Associated
Reference	(low)	(high)	p-value	Ratio	Allele
ra1801706	A = 0.15	A = 0.19	0.00765	0.661	G
	G = 0.85	G = 0.81
rs1047699	C = 0.85	C = 0.78	0.000594	1.65	C
	T = 0.15	T = 0.22
rs1948017	C = 0.14	C = 0.10	0.0188	1.51	C
	T = 0.86	T = 0.90
rs1498608	A = 0.09	A = 0.12	0.0347	0.672	T
	T = 0.91	T = 0.88
rs869975	A = 0.05	A = 0.10	0.000243	0.441	G
	G = 0.95	G = 0.90

The single marker alleles set forth in Table 7 were considered validated, since the genotyping data were significantly associated with low BMD, and because the genotyping results agreed with the original allelotyping results. Particularly significant associations with low BMD are indicated by a calculated p-value of less than 0.05 for genotype results, which are set forth in bold text.

Odds ratio results are shown in Table 7 (and other Tables below). An odds ratio is an unbiased estimate of relative risk which can be obtained from most case-control studies. Relative risk (RR) is an estimate of the likelihood of disease in the exposed group (susceptibility allele or genotype carriers) compared to the unexposed group (not carriers). It can be calculated by the following equation: RR=IA/Ia

IA is the incidence of disease in the A carriers and Ia is the incidence of disease in the non-carriers.

RR>1 indicates the A allele increases disease susceptibility.

RR<1 indicates the a allele increases disease susceptibility.

For example, RR=1.5 indicates that carriers of the A allele have 1.5 times the risk of disease than non-carriers, i.e., 50% more likely to get the disease.

Case-control studies do not allow the direct estimation of IA and Ia, therefore relative risk cannot be directly estimated. However, the odds ratio (OR) can be calculated using the following equation: OR=(nDAnda)/(ndAnDa)=pDA(1−pdA)/pdA(1−pDA), or

OR=((case f)/(1−case f)/((control f)/(1−control f)), where f=susceptibility allele frequency.

An odds ratio can be interpreted in the same way a relative risk is interpreted and can be directly estimated using the data from case-control studies, i.e., case and control allele frequencies. The higher the odds ratio value, the larger the effect that particular allele has on the development of low BMD. Possessing an allele associated with a relatively high odds ratio translates to having a higher risk of developing or having low BMD.

Example 3

CETP Region Proximal SNPs

It has been discovered that a polymorphic variation (rs1801706) in a gene encoding CETP is associated with the occurrence of low BMD (see Examples 1 and 2). Ninety-one additional allelic variants proximal to rs1801706 were identified and subsequently allelotyped in low BMD case and high BMD control sample sets as described in Examples 1 and 2. The polymorphic variants are set forth in Table 8. The chromosome positions provided in column four of Table 8 are based on Genome “Build 34” of NCBI's GenBank. The “genome letter” corresponds to the particular allele that appears in NCBI's build 34 genomic sequence of the region (chromosome 16: positions 56743155-56840953), and the “deduced iupac” corresponds to the single letter IUPAC code for the CTEP polymorphic variants as they appear in SEQ ID NO:1. Also, the “genome letter” may differ from the alleles (A1/A2) provided in Table 8 (and in subsequent Tables that provide the same information) if the genome letter is on one stand and the alleles are on the complementary strand, thus having different strand orientations (i.e., reverse vs forward).

TABLE 8
	Position in		Chromosome	Alleles	Genome	Deduced
dbSNP	SEQ ID NO: 1	Chromosome	Position	(A1/A2)	letter	iupac
7500979	205	16	56743155	g/a	g	R
2217332	1595	16	56744545	g/a	g	R
8044804	2650	16	56745600	c/t	a	R
2270835	5496	16	56748446	c/t	t	Y
2133783	5782	16	56748732	g/a	a	R
247609	5908	16	56748858	c/t	g	R
952440	7552	16	56750502	g/a	a	R
881598	9191	16	56752141	t/c	g	R
2291955	10127	16	56753077	g/a	t	Y
2518054	10345	16	56753295	g/a	g	R
866038	10399	16	56753349	t/c	c	Y
1436425	12028	16	56754978	g/a	a	R
173537	13355	16	56756305	a/g	t	Y
247611	13687	16	56756637	a/g	g	R
166017	14328	16	56757278	t/c	c	Y
173538	14746	16	56757696	c/t	t	Y
193694	14996	16	56757946	t/c	c	Y
7205692	19361	16	56762311	g/a	a	R
8048746	21775	16	56764725	g/a	a	R
247618	23250	16	56766200	g/a	g	R
183130	23810	16	56766760	c/t	c	Y
6499863	24464	16	56767414	g/a	g	R
4783961	27341	16	56770291	g/a	g	R
3816117	28605	16	56771555	c/t	t	Y
711752	28658	16	56771608	g/a	g	R
708272	28735	16	56771685	c/t	g	R
1864163	29680	16	56772630	g/a	g	R
4369653	29998	16	56772948	g/a	c	Y
1864165	32521	16	56775471	c/t	c	Y
891141	36170	16	56779120	t/g	g	K
891143	36427	16	56779377	c/t	t	Y
7205804	37336	16	56780286	g/a	g	R
5885	37718	16	56780668	c/t	c	Y
1532625	37748	16	56780698	a/g	c	Y
1532624	37926	16	56780876	t/g	c	M
289712	38752	16	56781702	g/a	c	Y
7499892	39037	16	56781987	c/t	c	Y
5883	39800	16	56782750	c/t	c	Y
289714	39898	16	56782848	c/t	g	R
158480	40674	16	56783624	c/t	g	R
289717	41835	16	56784785	c/t	g	R
4344729	42325	16	56785275	c/t	g	R
289718	42379	16	56785329	a/g	c	Y
289719	42388	16	56785338	g/a	t	Y
2033254	42432	16	56785382	c/t	t	Y
4784744	43632	16	56786582	g/a	g	R
291044	43899	16	56786849	c/t	g	R
8053613	44273	16	56787223	c/t	c	Y
5881	44459	16	56787409	a/g	g	R
5880	47538	16	56790488	c/g	g	S
7198026	47692	16	56790642	c/t	t	Y
5882	48539	16	56791489	g/a	g	R
8045701	48749	16	56791699	c/t	t	Y
289741	49921	16	56792871	a/g	g	R
1801706	50109	16	56793059	a/g	g	R
289742	50209	16	56793159	c/g	c	S
289743	50243	16	56793193	c/t	g	R
289746	52652	16	56795602	g/a	c	Y
172337	55195	16	56798145	c/t	t	Y
289747	56385	16	56799335	a/g	c	Y
1566439	57109	16	56800059	a/g	t	Y
7205459	57618	16	56800568	c/t	t	Y
289749	58741	16	56801691	t/c	g	R
289751	59222	16	56802172	t/c	g	R
8059220	60771	16	56803721	c/t	c	Y
8058353	60962	16	56803912	g/a	g	R
289735	62009	16	56804959	a/g	c	Y
289737	64589	16	56807539	t/g	a	M
291042	66054	16	56809004	g/a	a	R
1875236	66143	16	56809093	c/t	g	R
821466	67822	16	56810772	a/g	t	Y
821465	68805	16	56811755	c/g	c	S
4275846	70075	16	56813025	c/t	g	R
289707	70350	16	56813300	a/g	t	Y
821463	71214	16	56814164	g/a	t	Y
289706	79549	16	56822499	c/t	c	Y
1167741	82760	16	56825710	c/t	c	Y
2052880	86463	16	56829413	a/c	g	K
1167742	86533	16	56829483	c/g	c	S
1183256	87019	16	56829969	a/g	a	R
1651665	88910	16	56831860	a/c	a	M
1651666	88955	16	56831905	c/t	c	Y
4784751	89021	16	56831971	c/t	c	Y
1651667	89056	16	56832006	a/g	a	R
8052091	89863	16	56832813	g/a	g	R
1684574	89879	16	56832829	t/c	t	Y
1684575	90066	16	56833016	t/g	g	K
1672865	90101	16	56833051	a/g	a	R
821470	91029	16	56833979	g/a	a	R
1549669	91434	16	56834384	t/g	a	M
291040	93636	16	56836586	t/c	t	Y
289754	98003	16	56840953	c/t	c	Y

Assay for Verifying and Allelotyping SNPs

The methods used to verify and allelotype the proximal SNPs of Table 8 are the same methods described in Examples 1 and 2 herein. The primers and probes used in these assays are provided in Table 9 and Table 10, respectively.

TABLE 9
dbSNP
rs#	Forward PCR primer	Reverse PCR primer
7500979	TTGTATATGTAGGGTCCTGC	AAAATAATTCAAAACCACTG
2217332	ATCCAACAACAGCTTCCCAG	GGTGAAATGCTGACCTGTGT
8044804	ATAAATACAACCCAGCCCAC	CATTTCATTTCCCTGCACTG
2270835	CATTTACCATGTAACTGCCG	GGGTTGAGTATGAACAAATG
2133783	ACACTCATCCCTCCATTCTG	TCCCGGGAATTGAAGGGAAT
247609	GTCACTAAACTATGTATCAG	GGGTAGAGTGTAAATGACAG
952440	AGTCAGTGCCTGACTTTACC	CATACTGAGGCATGGAACAG
881598	ACTGCACTCCAGCCTGGGTGA	TGCTCCTGGCAGCAAATATC
2291955	GCTAGACATGTTTTAGCAGG	CCCCTTAGTTTAAAGTAAAGC
2518054	CTGATCATTCTTACCGGCAC	CTAGACAGTCAACAACTGAG
866038	CAAGGAACAAAGCAAGACCC	GTGCCGGTAAGAATGATCAG
1436425	GTATTCAGAGTGGTGTGTGG	GGGACATGGCAGAAATTCAG
173537	TCCCTGTCCTTGAACTCATC	CCTCTTGGGTCTTGTAGTCG
247611	AGGGTCCTCCATGATTGGAG	TTTCTGGACCTGACTGGGTG
166017	TAGATGGGCTGTTTCTACTG	AGGGAAGATTCCAGTAAAGC
173538	TCCCAAAGTGCTGGGATTAC	GGGTTTCTCAAAGGGCTAAG
193694	TCCTTCCGTGATCTCACAAC	CACACCAAAAGAACTGCATG
7205692	TCTTAGCCTGGGACTTTCTG	AAAAGCAGCTGTGACCTAAG
8048746	CAAGTTCTTCCTCCATCCAC	TTCCTCTCTCTGGGCTTATC
247618	TTTTCCCCCTTTTTGGGGC	TGGGTTGTCACAGCAAGGTGG
183130	ATAAACGGGAAAGAAGAGAC	AGGGTGGAGAATCTACAGAA
6499863	GTCTTGACAGGTTTGAGGAG	CATCAGACAGATCCCAACAG
4783961	GAAACATGAGTCGGGATGGC	AGCTTTGGTATTGGAGCAGG
3816117	ACTAGCCCAGAGAGAGGAGT	AAGAACTTGACCTTGAAGGC
711752	CACAAATCCCTATACCTGGC	TCGCCTTCAAGGTCAAGTTC
708272	AACCTGGCTCAGATCTGAAC	GCCAGGTATAGGGATTTGTG
1864163	TTAGAGGGGCTGTTGGAGAG	AGAGCCTGACACCTTCCCTA
4369653	AGTATCTTGACTTTATTTGG	GAAAAAAATATATGATAAAGG
1864165	TAAAAGGCTAGAAGTCCACC	AATTAATTCTCCCCTATAGC
891141	AGGCCCAGCCTGGGAAGTTT	TATCAGATGGTATCCACATG
891143	TCGTGCCCATCCTGTTAGTG	CACAAGCATGCCCTGTGTGG
7205804	AGGCAGCAAGCACCACAATG	ATGAACGGTGCCTGGTACAC
5885	GCAAAGAGATCAACGTCATC	ATGCAGACAGAAACGCACTCA
1532625	ACTGCTGTCTTCTGAGGCAT	CATCATGGCCGATTTTGTCC
1532624	TCTATAGACTTGCCCAACGC	TACTTTGGCAAATCTCTGCC
289712	ATTCGGCTTCTGTCATCCTC	GGGTCCAAAGCTTTTGTCAG
7499892	TGGCTGACTGGCCTGACCAC	CCCTCCATTCTGTACCACTTA
5883	TACTTCTGGTTCTCTGAGCG	TTGAACTCGTCTCCCATCAG
289714	ACACACACATACCACATGCC	TGATGGGAGACGAGTTCAAG
158480	TGAGTAGTTGGGACTATAGG	ACCCCTGTCTCTACAAAAAT
289717	CTCTGAGCCCAGAGTTGATC	CATTCCCTGCTCCATTTCCC
4344729	GCCTGAGTTCAGAAGGGAAG	GGCCGTTCTCCTGTTCTAAC
289718	GCCTGCCCAATATTGTGAGT	TCTTCCCTTCTGAACTCAGG
289719	TCTTCCCTTCTGAACTCAGG	GCCTGCCCAATATTGTGAGT
2033254	CACAACTCACAATATTGGGC	CAAAGGAACAGGACTCAGAC
4784744	ATCACATGCCCCAAGAAACC	CCGGCCCTTTCTTTCTTTTG
291044	AGGGCATCCCAGAACAGAAC	TGACTAGGTCAGGTCCCCTC
8053613	GAGTTCAGGGTAGGAATAGC	GCTGTGCAAATTAGGACTCT
5881	GACCCCTGTCTTCCACAGGT	ATCTTGGGCATCTTGAGGCA
5880	TTTCTCTCCCCAGGATATCG	CCAAGAGGCTTAAGAAGAGC
7198026	TGTTGGTGGGGAAATGTGGC	ACGAAGATTCTATCTAGGCA
5882	TCCAGGGAGGACTCACCATG	TGACTGCAGGAAGCTCTGG
8045701	TTGATACTTAGCGGTCCTGG	TAATATTCTGCAGGTAAACC
289741	TCTACCAGCTTGGCTCCCTC	AAGGGAGGGGCAGTAGGAGA
1801706	GTAGCAGAAGGCAAGCAC	AGGGAGGAGTTGGGAGCC
289742	ACTGGTGAGACAATCCCTTC	CCACTGGCATTAAAGTGCTG
289743	TGAAAAGAGGTGGACGGCAC	AGTCCTTCTTCTGTGGCTGG
289746	GAGGCTTACCAAAATGGGAC	AGAGCTTCTAGGCTTGGATG
172337	AGCTGGACTTTGAGGATGGC	AAGGAGAGGAGGGACTGGAG
289747	GTTGTTTAGGCCAAAAAGTC	CAATTACGGAAGTTACACTG
1566439	AAGCCCATGGCTTTTCAAGG	AGATCCTGGAGCCTCATTGT
7205459	GCAGAGGGGAAAGATCTTGG	AAGAGGGTATGTGTGTTGG
289749	TGAACTAAGGACCAGGCAAG	GCTCACATCCTTAGATTGCC
289751	AAATGATATGGAATTATGCG	TATCTTTCCAAATGTTTTAC
8059220	ACCTGCCATCATGAGTGCAT	TAGAGCAGGGCCTGGTGTGT
8058353	CCCTACAAGAAGGCTACATC	AGTGACTGCAGTGAAGAAGG
289735	CAAGGCATGCATGCTCCTTC	ATCGCCAGACCCTCAAGAAC
289737	GCAAGGAAGACTGATTCGTG	AGCCTCAAGTCACTGATGTC
291042	TGTCTCCCCATAACCAACAC	AAGAAACAGCTCCCAGTCTG
1875236	GAGCTGAGTGATTCTTGTCC	AATATGGTCAACTTGGTGGC
821466	AAGGAAGGAAGTGAGGGATG	GGAAAGCAAAGGAATGGCAG
821465	CACTGTTTACAATAGCCAAG	TGGAATTACAGGCGTGAGCT
4275846	GCAGCTGAGCAAAGATATGG	TGGACCTCTTCTATCATCAC
289707	CTCCTTCCTTCTGCTATCTC	ACCAAGATTCAGGCACTGAG
821463	CGTCTGCTGGGACACTGAAA	GCACCTGGAAGATTTATGGG
289706	TTAAGTCCTTTACCAAAGGG	TGAATGTGGCTTTACCAAGG
1167741	GCCCTATTTACGTGGATTTG	GCTCTGATTGTGTCTCTGTG
2052880	CATCAAGACTCCATGGAGAG	AGTCAAGAACCAGTCCCTAC
1167742	CCCTAGAAACTCCCTTATCC	TGAGTGGGGTACAGATGAAC
1183256	AGCTCAACTCCCCCCAAGTT	GAAGATAGGTGAGTTGAGGG
1651665	AAGTGAGAACAGGCTTCCTG	TCAAGGCTTTAGCTTGTCCC
1651666	TCTAACCTGGAACCCCTCAG	ATGAAGCCTGACACTTTGGG
4784751	CAAAGTGTCAGGCTTCATGG	AGCTGTGGGTGAGTGTCAAG
1651667	TCCCGACTTGTACACCTTGG	AGAAAATCAGCCAAAGGCTC
8052091	AGTCTTGCAGTCCAGGATG	TTCCCTGATGGACAGATGGC
1684574	TCTTGCAGTCCAGGATGCAG	GGCCATTTAGTGCTTGGAGC
1684575	TTTCCAGTCCCTGCATGTG	GAGGGGTTACCCAGAACCTT
1672865	AAAGGTTCTGGGTAACCCCT	CAGGCCTGAAAAAGCAAAGG
821470	CCTCTTTCTGTAATATCTGG	CTGGGCATTGCAGAACTGAA
1549869	CTGCAATAGTACACGTGGTG	AGATGTTGCAAAGGAGGTGC
291040	TACACTTGCCCAAAGTCCAC	TCTCTCTGTCAATCATGGGC
289754	ACTGCTTAGGTTGGCAAAGG	ATGCTTCCTTCCACAGGGAC

TABLE 10
dbSNP rs#	Extend Primer	Term Mix
7500979	TCAACCAATAGAAAAGGC	ACG
2217332	AACCTCTGGTCCTCTGGA	ACG
8044804	TAAGCCTTGGTATGATAC	ACG
2270835	GCCGTAAATTCCATTCTTC	ACG
2133783	CTGAACTTTACAGGTAACA	ACG
247609	AAACTATGTATCAGACAAAAGCAC	ACG
952440	CCTGGAAGGCAGCTGTGG	ACG
881598	CAGCCTGGGTGACAGAGC	ACT
2291955	GACTTTTCTAGGAAAGACTTA	ACG
2518054	TACCGGCACAAACAGTC	ACG
866038	CCCATCTCAAAAACAAAAAC	ACT
1436425	GGTGTGTGGCCCATGAT	ACG
173537	CATCACACCTGTCTGCCATC	ACT
247611	CTCCATGATTGGAGACTGACA	ACT
166017	CTACTGGCAGGGAACAT	ACT
173538	TTACAGGCGTAAGCCAC	ACG
193694	CTTAAAATCTACTCCCATACAT	ACT
7205692	CAAAAGAGTTAGGGGAG	ACG
8048746	CATGCCAAAATCTCGCC	ACG
247618	TTGGGGCCCCATGTAAA	ACG
183130	CTTCTGTGCAGGAGAAT	ACG
6499863	TTGAGGAGCAGTGGTCA	ACG
4783961	GGGTCCTGCCCTAGTCC	ACG
3816117	GAGGGAGATGGGCTGAG	ACG
711752	CAATGCAGCTAGGACCTTCT	ACG
708272	CTGGCTCAGATCTGAACCCTAACT	ACG
1864163	GGCTGTTGGAGAGGTTGATA	ACG
4369653	CAGGTGCTTTTACAAACAA	ACG
1864165	GTCCACCATGGCCCTCC	ACG
891141	CTGGGAAGTTTGCAGGG	ACT
891143	AGTGTGTCCACGGCTCC	ACG
7205804	CCTGTGCATCCATGGAG	ACG
5885	CATCTCTAACATCATGGC	ACG
1532625	TGAGGCATGCAGACAGAAAC	ACT
1532624	CAACGCCACACAGCTTGTGA	ACT
289712	CAGTGGATTGTGGCCCCC	ACG
7499892	AGCCCGTTGGCCTGAAC	ACG
5883	GTTCTCTGAGCGAGTCTT	ACG
289714	CATACCACATGCCATCTGGAT	ACG
158480	GGGACTATAGGTATGCAC	ACG
289717	TTCCAGCCCCCTACAAGTCACG	ACG
4344729	CAGAAGGGAAGAGGGAC	ACG
289718	TATGTGCAAGGAGAGAG	ACT
289719	CTGTGATGCCTCTCTCC	ACG
2033254	GGCAGGCCCCTGACCGGC	ACG
4784744	CCAAGAAACCACTGAAC	ACG
291044	AGTATTTAAAGGAGAGACACAC	ACG
8053613	GATGAGAAACTGAGGCC	ACG
5881	TTCCACAGGTTGTCGGC	ACT
5880	ATATCGTGACTACCGTCCAG	ACT
7198026	GAAATGTGGCCCCTTTC	ACG
5882	CAGAGCAGCTCCGAGTCC	ACT
8045701	CCTCTCCCTGCTGGTGG	ACG
289741	TGGGAGTCAGCCCAGCTC	ACT
1801706	CCTGGTGTCTCCTCCAGC	ACT
289742	GCCACAGAAGAAGGACTCC	ACT
289743	TGAGACAATCCCTTCCCCC	ACG
289746	TACCAAAATGGGACTGACCTC	ACG
172337	TTTGAGGATGGCCCCTGACACG	ACG
289747	TAGGCCAAAAAGTCTAAATTGC	ACT
1566439	CCTTAGAGAAACGGAAGGTG	ACT
7205459	ACTTTATTCCTGGATCAGA	ACG
289749	CAGGCAAGCTAATGCAA	ACT
289751	TGGAATTATGCGTTAAAGG	ACT
8059220	TGCATTCAGCTTCTCCA	ACG
8058353	CTACATCTGCCTCTCTATC	ACG
289735	GAGGGACAGAAGGGACC	ACT
289737	CTTACTTGTGTGGTTTAGAAT	ACT
291042	CCCATAACCAACACTCAGCT	ACG
1875236	GATTCTTGTCCTCTGAGAGC	ACG
821466	GAGGACAGGAGTGGAGCC	ACT
821465	ACAGAGAAAATGTGGCA	ACT
4275846	GATATGGTTTCAAAAGCCT	ACG
289707	TGCCATTCTCTAGGCCCGTC	ACT
821463	TGGGACACTGAAACCAGGG	ACG
289706	ACCAAAGGGATTGACTT	ACG
1167741	GAACTCTGAGTTACATTCC	ACG
2052880	CTTCCCTGCCTATTTTATGTC	ACT
1167742	CTTATCCCCCAACTCACCTT	ACT
1183256	TCATCTGCCCCCAGTTT	ACT
1651665	AGGGGTTCCAGGTTAGACCCTT	ACT
1651666	AGGAAGCCTGTTCTCACTTTC	ACG
4784751	TCATGGGGAAGCCCTAA	ACG
1651667	CTTGTACACCTTGGACTTGAC	ACT
8052091	CAACAGCCAGCAGCCCC	ACG
1684574	GATGCAGGAAGCAGGGCC	ACT
1684575	CACCTGTCTGTGGACTGGA	ACT
1672865	GGGTAACCCCTCAGCCCAG	ACT
821470	TCTGGATCCCCAGTGCC	ACG
1549669	TAGTACACGTGGTGTAAGGG	ACT
291040	CCAAAGTCCACCAGCCTCTACT	ACT
289754	CAAAGGATTTTAACTTCCTCTAG	ACG

Genetic Analysis

Allelotyping results are shown for female cases and controls in Table 11. The allele frequency for the A2 allele is noted in the fifth and sixth columns for control pools and case pools, respectively, where “AF” is allele frequency. Some SNPs do not have an allele frequency disclosed because of failed assays.

TABLE 11
	Position in							Low BMD
dbSNP	SEQ ID	Chromosome	A1/A2					Associated
rs#	NO: 1	Position	Allele	High AF	Low AF	p-Value	OR	Allele
7500979	205	56743155	G/A	G = 0.71	G = 0.72	0.6029	1.07	G
				A = 0.29	A = 0.28
2217332	1595	56744545	G/A	G = 0.85	G = 0.84	0.5390	0.90	A
				A = 0.15	A = 0.16
8044804	2650	56745600	C/T	C = 0.47	C =
				T = 0.53	T =
2270835	5496	56748446	C/T	C = 0.00	C = 0.00
				T = 1.00	T = 1.00
2133783	5782	56748732	G/A	G = 0.41	G = 0.44	0.3412	1.14	G
				A = 0.59	A = 0.56
247609	5908	56748858	C/T	C = 0.70	C = 0.70	0.8844	1.02	C
				T = 0.30	T = 0.30
952440	7552	56750502	G/A	G = 0.31	G = 0.30	0.8410	0.97	A
				A = 0.69	A = 0.70
881598	9191	56752141	T/C	T = 0.23	T = 0.25	0.4703	1.11	T
				C = 0.77	C = 0.75
2291955	10127	56753077	G/A	G = 0.00	G = 0.00
				A = 1.00	A = 1.00
2518054	10345	56753295	G/A	G = 0.87	G = 0.91	0.0835	1.47	G
				A = 0.13	A = 0.09
866038	10399	56753349	T/C	T = 0.49	T = 0.51	0.5835	1.07	T
				C = 0.51	C = 0.49
1436425	12028	56754978	G/A	G = 0.39	G = 0.38	0.7660	0.96	A
				A = 0.61	A = 0.62
173537	13355	56756305	A/G	A = 0.80	A = 0.81	0.8011	1.04	A
				G = 0.20	G = 0.19
247611	13687	56756637	A/G	A = 0.38	A = 0.39	0.7529	1.04	A
				G = 0.62	G = 0.61
166017	14328	56757278	T/C	T = 0.29	T = 0.43	0.00001	1.80	T
				C = 0.71	C = 0.57
173538	14746	56757696	C/T	C = 0.68	C = 0.67	0.5702	0.93	T
				T = 0.32	T = 0.33
193694	14996	56757946	T/C	T = 0.14	T = 0.24	0.0015	2.02	T
				C = 0.86	C = 0.76
7205692	19361	56762311	G/A	G = 0.01	G = 0.01
				A = 0.99	A = 0.99
8048746	21775	56764725	G/A	G = 0.00	G = 0.00
				A = 1.00	A = 1.00
247618	23250	56766200	G/A	G = 0.28	G = 0.29	0.5339	1.08	G
				A = 0.72	A = 0.71
183130	23810	56766760	C/T	C = 0.64	C = 0.59	0.0720	0.80	T
				T = 0.36	T = 0.41
6499863	24464	56767414	G/A	G = 0.83	G = 0.84	0.5810	1.09	G
				A = 0.17	A = 0.16
4783961	27341	56770291	G/A	G = 0.48	G = 0.46	0.3803	0.90	A
				A = 0.52	A = 0.54
3816117	28605	56771555	C/T	C = 0.56	C = 0.55	0.8019	0.97	T
				T = 0.44	T = 0.45
711752	28658	56771608	G/A	G = 0.59	G = 0.58	0.6887	0.95	A
				A = 0.41	A = 0.42
708272	28735	56771685	C/T	C = 0.62	C = 0.62	0.9629	1.01	C
				T = 0.38	T = 0.38
1864163	29680	56772630	G/A	G = 0.74	G = 0.74	0.9938	1.00	G
				A = 0.26	A = 0.26
4369653	29998	56772948	G/A	G = 0.37	G = 0.37	0.8163	0.97	G
				A = 0.63	A = 0.63
1864165	32521	56775471	C/T	C = 1.00	C = 1.00
				T = 0.00	T = 0.00
891141	36170	56779120	T/G	T = 1.00	T = 1.00
				G = 0.00	G = 0.00
891143	36427	56779377	C/T	C = 1.00	C = 1.00
				T = 0.00	T = 0.00
7205804	37336	56780286	G/A	G = 0.72	G = 0.78	0.0212	1.41	G
				A = 0.28	A = 0.22
5885	37718	56780668	C/T	C = 1.00	C = 1.00
				T = 0.00	T = 0.00
1532625	37748	56780698	A/G	A = 0.60	A = 0.61	0.6329	1.06	A
				G = 0.40	G = 0.39
1532624	37926	56780876	T/G	T = 0.64	T = 0.64	0.9807	1.00	T
				G = 0.36	G = 0.36
289712	38752	56781702	G/A	G = 0.66	G = 0.67	0.6644	1.06	G
				A = 0.34	A = 0.33
7499892	39037	56781987	C/T	C = 0.88	C = 0.88	0.9632	1.01	C
				T = 0.12	T = 0.12
5883	39800	56782750	C/T	C = 0.95	C = 0.96	0.8994	1.04	C
				T = 0.05	T = 0.04
289714	39898	56782848	C/T	C = 0.19	C = 0.23	0.0972	1.28	C
				T = 0.81	T = 0.77
158480	40674	56783624	C/T	C = 0.59	C = 0.56	0.3315	0.86	T
				T = 0.41	T = 0.44
289717	41835	56784785	C/T	C = 0.67	C = 0.72	0.1148	1.25	C
				T = 0.33	T = 0.28
4344729	42325	56785275	C/T	C = 1.00	C = 1.00
				T = 0.00	T = 0.00
289718	42379	56785329	A/G	A =	A = 0.20
				G =	G = 0.80
289719	42388	56785338	G/A	G =	G = 0.39
				A =	A = 0.61
2033254	42432	56785382	C/T	C = 0.34	C = 0.38	0.1947	1.17	C
				T = 0.66	T = 0.62
4784744	43632	56786582	G/A	G = 0.63	G = 0.64	0.5113	1.08	G
				A = 0.37	A = 0.36
291044	43899	56786849	C/T	C = 0.65	C = 0.65	0.9666	1.01	C
				T = 0.35	T = 0.35
8053613	44273	56787223	C/T	C = 1.00	C = 1.00
				T = 0.00	T = 0.00
5881	44459	56787409	A/G	A = 0.00	A = 0.00
				G = 1.00	G = 1.00
5880	47538	56790488	C/G	C = 0.09	C = 0.09	0.9339	1.02	C
				G = 0.91	G = 0.91
7198026	47692	56790642	C/T	C = 0.00	C = 0.00			C
				T = 1.00	T = 1.00
5882	48539	56791489	A/G	A = 0.76	A = 0.80	0.0958	1.27	A
				G = 0.24	G = 0.20
8045701	48749	56791699	C/T	C = 0.00	C = 0.00
				T = 1.00	T = 1.00
289741	49921	56792871	A/G	A = 0.85	A = 0.86	0.5974	1.09	A
				G = 0.15	G = 0.14
1801706	50109	56793059	A/G	A = 0.31	A = 0.23	0.0025	0.67	G
				G = 0.69	G = 0.77
289742	50209	56793159	C/G	C = 0.91	C = 0.92	0.6298	1.12	C
				G = 0.09	G = 0.08
289743	50243	56793193	C/T	C = 0.27	C = 0.24	0.1869	0.84	T
				T = 0.73	T = 0.76
289746	52652	56795602	G/A	G = 0.72	G = 0.72	0.8916	0.98	G
				A = 0.28	A = 0.28
172337	55195	56798145	C/T	C = 0.94	C = 0.91	0.0886	0.67	T
				T = 0.06	T = 0.09
289747	56385	56799335	A/G	A = 0.49	A = 0.54	0.0981	1.22	A
				G = 0.51	G = 0.46
1566439	57109	56800059	A/G	A = 0.66	A = 0.65	0.8578	0.98	G
				G = 0.34	G = 0.35
7205459	57618	56800568	C/T	C = 0.16	C = 0.10	0.0035	0.59	T
				T = 0.84	T = 0.90
289749	58741	56801691	T/C	T =	T = 0.42
				C =	C = 0.58
289751	59222	56802172	T/C	T = 0.98	T = 0.94
				C = 0.02	C = 0.06
8059220	60771	56803721	C/T	C = 1.00	C = 1.00
				T = 0.00	T = 0.00
8058353	60962	56803912	G/A	G = 0.98	G = 0.95
				A = 0.02	A = 0.05
289735	62009	56804959	A/G	A =	A = 0.39
				G =	G = 0.61
289737	64589	56807539	T/G	T =	T = 0.28
				G =	G = 0.72
291042	66054	56809004	G/A	G = 0.80	G = 0.82	0.4548	1.12	G
				A = 0.20	A = 0.18
1875236	66143	56809093	C/T	C = 0.91	C = 0.90	0.3106	0.80	T
				T = 0.09	T = 0.10
821466	67822	56810772	A/G	A = 0.77	A = 0.76	0.6743	0.94	G
				G = 0.23	G = 0.24
821465	68805	56811755	C/G	C = 0.93	C = 0.87	0.0041	0.54	G
				G = 0.07	G = 0.13
4275846	70075	56813025	C/T	C = 1.00	C = 1.00
				T = 0.00	T = 0.00
289707	70350	56813300	A/G	A = 0.44	A = 0.46	0.6117	1.07	A
				G = 0.56	G = 0.54
821463	71214	56814164	G/A	G = 0.38	G = 0.41	0.4281	1.10	G
				A = 0.62	A = 0.59
289706	79549	56822499	C/T	C = 0.00	C = 0.00
				T = 1.00	T = 1.00
1167741	82760	56825710	C/T	C = 0.45	C = 0.46	0.7635	1.04	C
				T = 0.55	T = 0.54
2052880	86463	56829413	A/C	A = 0.67	A = 0.68	0.8378	1.03	A
				C = 0.33	C = 0.32
1167742	86533	56829483	C/G	C = 0.70	C = 0.69	0.7322	0.96	G
				G = 0.30	G = 0.31
1183256	87019	56829969	A/G	A = 1.00	A = 1.00
				G = 0.00	G = 0.00
1651665	88910	56831860	A/C	A = 0.30	A = 0.35	0.1423	1.24	A
				C = 0.70	C = 0.65
1651666	88955	56831905	C/T	C = 0.71	C = 0.67	0.1294	0.81	T
				T = 0.29	T = 0.33
4784751	89021	56831971	C/T	C = 0.70	C = 0.73	0.1971	1.18	C
				T = 0.30	T = 0.27
1651667	89056	56832006	A/G	A = 0.44	A = 0.47	0.2902	1.13	A
				G = 0.56	G = 0.53
8052091	89863	56832813	G/A	G = 1.00	G = 1.00
				A = 0.00	A = 0.00
1684574	89879	56832829	T/C	T = 0.01	T =
				C = 0.99	C =
1684575	90066	56833016	T/G	T = 0.65	T = 0.67	0.3473	1.12	T
				G = 0.35	G = 0.33
1672865	90101	56833051	A/G	A = 0.49	A = 0.50	0.6959	1.05	A
				G = 0.51	G = 0.50
821470	91029	56833979	G/A	G = 0.37	G = 0.38	0.8340	1.03	G
				A = 0.63	A = 0.62
1549669	91434	56834384	T/G	T = 0.51	T = 0.50	0.9075	0.99	G
				G = 0.49	G = 0.50
291040	93636	56836586	T/C	T = 0.76	T = 0.74	0.4056	0.89	C
				C = 0.24	C = 0.26
289754	98003	56840953	C/T	C = 0.70	C = 0.69	0.6508	0.94	T
				T = 0.30	T = 0.31

Allelotyping results were considered particularly significant with a calculated p-value of less than or equal to 0.05 for allelotype results. These values are indicated in bold. The allelotyping p-values were plotted in FIG. 1. The position of each SNP on the chromosome is presented on the x-axis. The y-axis gives the negative logarithm (base 10) of the p-value comparing the estimated allele in the case group to that of the control group. The minor allele frequency of the control group for each SNP designated by an X or other symbol on the graphs in FIG. 1 can be determined by consulting Table 11. For example, the left-most X on the left graph is at position 56743155. By proceeding down the table from top to bottom and across the graphs from left to right the allele frequency associated with each symbol shown can be determined.

To aid the interpretation, multiple lines have been added to the graph. The broken horizontal lines are drawn at two common significance levels, 0.05 and 0.01. The vertical broken lines are drawn very 20 kb to assist in the interpretation of distances between SNPs. Two other lines are drawn to expose linear trends in the association of SNPs to the disease. The light gray line (or generally bottom-most curve) is a nonlinear smoother through the data points on the graph using a local polynomial regression method (W. S. Cleveland, E. Grosse and W. M. Shyu (1992) Local regression models. Chapter 8 of Statistical Models in S eds J. M. Chambers and T. J. Hastie, Wadsworth & Brooks/Cole). The black line provides a local test for excess statistical significance to identify regions of association. This was created by use of a 10 kb sliding window with 1 kb step sizes. Within each window, a chi-square goodness of fit test was applied to compare the proportion of SNPs that were significant at a test wise level of 0.01, to the proportion that would be expected by chance alone (0.05 for the methods used here). Resulting p-values that were less than 10⁻⁸ were truncated at that value.

Finally, the exons and introns of the genes in the covered region are plotted below each graph at the appropriate chromosomal positions. The gene boundary is indicated by the broken horizontal line. The exon positions are shown as thick, unbroken bars. An arrow is place at the 3′ end of each gene to show the direction of transcription.

Example 4

PROL4 Proximal SNPs

It has been discovered that a polymorphic variation (rs1047699) in a gene encoding PROL4 is associated with the occurrence of low BMD (see Examples 1 and 2). One hundred twenty-five additional allelic variants proximal to rs1047699 were identified and subsequently allelotyped in low BMD case and high BMD control sample sets as described in Examples 1 and 2. The polymorphic variants are set forth in Table 12. The chromosome position provided in column four of Table 12 is based on Genome “Build 34” of NCBI's GenBank.

TABLE 12
	Position in
	SEQ ID		Chromosome	Alleles
dbSNP	NO: 2	Chromosome	Position	(A1/A2)	genome_letter	deduced_iupac
523051	229	12	10842129	t/c	g	R
693620	368	12	10842268	t/c	a	R
2588349	583	12	10842483	g/a	g	R
2588350	2424	12	10844324	t/c	t	Y
619381	3625	12	10845525	c/t	c	Y
3759252	3709	12	10845609	c/a	g	K
3759251	3750	12	10845650	t/a	t	W
2418107	4724	12	10846624	g/c	g	S
7303054	4781	12	10846681	t/c	c	Y
1838345	6161	12	10848061	a/g	g	R
620878	7097	12	10848997	t/g	g	K
2537817	8025	12	10849925	t/c	t	Y
1548803	8398	12	10850298	c/t	c	Y
667123	10144	12	10852044	g/a	a	R
1838346	10384	12	10852284	a/g	g	R
2159903	11116	12	10853016	a/g	g	R
3944035	11132	12	10853032	a/g	g	R
3741845	11482	12	10853382	t/c	a	R
2110096	14544	12	10856444	c/t	c	Y
759055	15688	12	10857588	t/a	t	W
589377	17311	12	10859211	g/c	g	S
7960194	17831	12	10859731	g/t	t	K
7978242	20012	12	10861912	a/g	g	R
601051	21997	12	10863897	g/a	g	R
4262797	22861	12	10864761	a/g	a	R
2215714	23470	12	10865370	a/g	g	R
1373434	23515	12	10865415	g/a	g	R
2215715	23863	12	10865763	g/a	g	R
612456	24108	12	10866008	c/a	c	M
612808	24138	12	10866038	t/c	c	Y
689118	26469	12	10868369	t/c	t	Y
597468	27769	12	10869669	a/g	g	R
592864	29683	12	10871583	t/c	t	Y
640372	30491	12	10872391	a/c	c	M
7966559	30745	12	10872645	a/t	a	W
654834	31429	12	10873329	t/c	t	Y
4763216	31779	12	10873679	c/g	c	S
668521	32194	12	10874094	a/c	c	M
669503	32441	12	10874341	t/c	c	Y
3906864	32454	12	10874354	t/c	g	R
3906863	32459	12	10874359	c/t	a	R
7957888	35151	12	10877051	a/t	t	W
9300230	35362	12	10877262	a/t	t	W
7306214	35630	12	10877530	g/c	g	S
763839	36930	12	10878830	g/c	g	S
2418105	37490	12	10879390	g/a	g	R
666841	38432	12	10880332	c/t	c	Y
3851578	38688	12	10880588	a/g	g	R
7138797	39524	12	10881424	t/c	c	Y
7295252	41580	12	10883480	a/c	a	M
2418106	42531	12	10884431	t/c	t	Y
7299578	42665	12	10884565	a/g	a	R
621112	43038	12	10884938	a/g	a	R
3863320	44183	12	10886083	g/a	g	R
1373432	46271	12	10888171	a/t	t	W
1047699	49075	12	10890975	c/t	t	Y
1063193	49147	12	10891047	c/t	c	Y
2232959	49180	12	10891080	c/g	g	S
2227296	50301	12	10892201	a/g	a	R
1548804	50773	12	10892673	g/a	g	R
2232958	51243	12	10893143	g/a	c	Y
2232957	51530	12	10893430	t/c	a	R
2232956	52107	12	10894007	a/g	c	Y
1972571	52821	12	10894721	a/g	a	R
3759250	53341	12	10895241	t/c	a	R
3759249	53376	12	10895276	c/g	g	S
1541525	54047	12	10895947	c/t	t	Y
2098248	54392	12	10896292	t/c	t	Y
2900550	54482	12	10896382	t/a	a	W
7302130	55468	12	10897368	c/a	a	M
4763583	56990	12	10898890	a/g	a	R
4360778	57712	12	10899612	a/t	a	W
1607695	59667	12	10901567	c/t	a	R
1607694	59684	12	10901584	t/c	g	R
2192139	62043	12	10903943	a/c	a	M
7978300	63293	12	10905193	t/c	t	Y
7397871	63485	12	10905385	g/t	g	K
4763217	63778	12	10905678	c/t	t	Y
2159900	64222	12	10906122	a/g	a	R
10772370	65722	12	10907622	a/g	a	R
7398682	66315	12	10908215	c/t	c	Y
2900551	66829	12	10908729	a/g	a	R
2900552	66966	12	10908866	c/t	c	Y
2418214	66971	12	10908871	a/c	c	M
2418215	67013	12	10908913	t/c	c	Y
965243	70375	12	10912275	a/t	t	W
1117548	74118	12	10916018	t/a	t	W
1520225	75224	12	10917124	c/t	c	Y
1520226	75236	12	10917136	t/c	t	Y
1520227	75246	12	10917146	g/c	g	S
971919	75812	12	10917712	c/a	a	M
2159901	78968	12	10920868	c/t	t	Y
2159902	78998	12	10920898	t/c	t	Y
2110099	79328	12	10921228	a/c	c	M
7314847	80922	12	10922822	t/c	t	Y
7296003	81055	12	10922955	t/c	c	Y
4281556	81412	12	10923312	g/a	a	R
4763219	81785	12	10923685	a/g	g	R
3851579	82079	12	10923979	g/a	a	R
3851580	82087	12	10923987	g/a	g	R
1049119	82958	12	10924858	a/g	c	Y
2298866	83351	12	10925251	t/g	a	M
2298865	83442	12	10925342	c/t	a	R
2298864	83472	12	10925372	a/c	t	K
2298863	83966	12	10925866	a/g	t	Y
3180393	84414	12	10926314	t/c	t	Y
2070837	86563	12	10928463	c/g	c	S
7956204	86788	12	10928688	g/a	g	R
2418216	86796	12	10928696	t/g	t	K
3741844	87634	12	10929534	g/a	c	Y
4262798	88530	12	10930430	g/a	a	R
2418217	89202	12	10931102	a/g	a	R
2418218	89632	12	10931532	t/c	t	Y
7137492	89697	12	10931597	t/c	t	Y
2110100	89723	12	10931623	g/a	g	R
1013312	91063	12	10932963	c/g	c	S
4579993	91335	12	10933235	c/g	g	S
1013313	91504	12	10933404	t/c	t	Y
7397106	91619	12	10933519	c/t	t	Y
2215716	93715	12	10935615	a/g	g	R
2192140	93945	12	10935845	t/c	c	Y
4763589	94235	12	10936135	t/c	t	Y
1468697	95851	12	10937751	t/c	c	Y
2070837	130860	12	10972760	c/g	c	S
3180393	132815	12	10974715	t/c	a	R
2298865	133778	12	10975678	c/t	t	Y

Assay for Verifying and Allelotyping SNPs

The methods used to verify and allelotype the proximal SNPs of Table 12 are the same methods described in Examples 1 and 2 herein. The primers and probes used in these assays are provided in Table 13 and Table 14, respectively.

TABLE 13
dbSNP	Forward	Reverse
rs#	PCR primer	PCR primer
523051	CTCTGCTCAGAGCATAGATG	TTCCATGCATTTTAACCCCG
693820	GTAGGTCAATAAAAGAGGAGG	TTAGGCCGAGATTTTCAGAG
2588349	GTTCAAGAGTTTGATGTCAAG	CAGGGCATCTAAATTGAACG
2588350	TGTATCTGGGACATACCATC	GTTAGTACAGGGTTGATCAC
619381	AAAGCATAGTTTCTCTTCAG	GCATCTCTAAAGGTGATTTGG
3759252	GCTGTGATTTTTGGTGAGTC	TATTGTTCCCCAGTATTAGG
3759251	TTGTCCTTTCTCATTGCCAC	ATTAGAGCTATGGACTCACC
2418107	AGGTAAATGGGCATACAGTG	TGAAGACTGGAGCTATCTTC
7303054	AGGGAATTGGCCCAATTTGC	CCATTTACCTACTCAGCAGG
1838345	CCCAGGTTCAAGTGATTCTC	AATTAGCCAGGCATGGTTGC
620878	CCAGACCAAACGTTTCAATAA	AAGAAGAAACGAATGTTTAC
2537817	GTCAGAATGCTGACATGTAG	GGGGAAAAATGAGAATAGAC
1548803	ATGGGACAATTGCAAACAGG	ACTGAAATGTTCCATGTGAG
667123	TAAATACAAGAAGCCCTAGG	AGGGAAAGTACAACTTAGCC
1838346	GTTAGAAGGCTAGAGAGAAC	TTCATGAGTACTCTAGCTGG
2159903	AGTCAGGATACTCTTTAGGG	GGGAAGCTTTTCTGAAGATG
3944035	GGGTCTCTATGGAACAAAAG	TGAGGGAAGCTTTTCTGAAG
3741845	TGCTTGGTGTGTCTAACTAG	CAAACAGTTAACCCTGAACC
2110096	ATCAAGAACAGGATACTGCG	GGGTAGGAGATAAAGTCAGC
759055	CCCCTATCTTTTTGTTGGATG	GCTCTGTTTTCATGAGGTTC
589377	TTCACTGTTCAGATTGCTGG	ACTCCAGTTGTAGGTAGAAG
7960194	TGCTATCTTCCATGGAAGAA	TTTTTTTCCCTGTATGCCTC
7978242	GACGCTAAGCATCATTAGTC	TAGCCATTCTAGTGGATGTG
601051	TTTTTACGTCTGTGTGAACC	CACAGAAACCGAAAACCAAC
4262797	ATTTTAGTGCACCCATCACC	GTCTTTAGAGACTCAAAAGGG
2215714	GAGTTAAACATCAGTCGTTGT	TTATTATATAACGTAGGAA
1373434	CGTTTAAGATGATAGATCTTC	CTACGCAATAGGCTATTTTC
2215715	ACATGGATGGAAAGGAAGAC	TATGAGCGAGAATATGTGGC
612456	TATCAACAAGCCAAGATCCC	CTTTAGCCTTTTGTTGGACG
612808	GGCTGAGTGTTAAGTATTCC	AAGGCAAACCCTTAATAGGC
689118	GAAATGTTCTGTCTTCAGGC	AACGTTTCTGTGAGTGCAAG
597468	TACATGTTGAGATCCCAGAC	TGCATACATTGCGAAGATTC
592864	AAATTGTGTTTCTTGTAAGC	AATGAACTAAATACTCCAAG
640372	ATCTGAAACTCTTTGAGCGC	TCCAAAATGTTCCAATGAGC
7966559	CCTAATTCTTTTCTATCGGTG	GAAGCTAGAAAGCTAAGAGTG
654834	CTGTCCTTTTTCTGGCTTTC	CAAACAAAATAGAGTCCAGCG
4763216	TTGTAACCATAGAAAGCAGG	TCCCTCTATTTTTCTACTAC
668521	TGCTGCTGATGTAACTGCAG	GTGCAATAGTAACTGGGCTC
669503	GTAATCCACTCATTCAGATC	TACCTAGAGGAAATAAGTGG
3906864	TTTCTAACATCCACCTTCTG	GGTGTGAATAAGTTGTAATCC
3906863	CAGGACTGACCTCTAAAATAG	ACATCCACTTTCTGTAGATC
7957888	ACCTTAACAAAGTTCTGTGG	GAGCCACGAAAAAAAAAATG
9300230	CCAGACAAGCTAAAATACAG	CAAATACACACAGACACATC
7306214	CTATAGCACAGTAGGGTGAC	TCTTCGTGTTGGGCACATTC
763839	TATTCATGTAACACCGAAGG	GGAACAGCAAACTGAAACAG
2418105	GGAGGCATTCCATTCTTTTC	GTGTATCCAAAGCTTTAGTG
666841	TAATCCCCAAGAGAGAGGAC	AACTAACACAGATGGCCACC
3851578	GTGTGTATTTGCCCTTCTGC	GGCATGTGATAGGAATGTGC
7138797	GTTCTGAAGTACATGTGCAG	TTAATACCTAGGTGATGGGC
7295252	CAAGTTTACAATCACAGCTG	GGTAAATGTATTTGACAGTTG
2418106	CAGCCCAGAATGGCTTTGAA	ACTAACGATGGGTGATGAGC
7299578	ATCTGCCCCATGATCCAATC	CCCCTGAAAACCTCATGTTG
621112	TATGTTGGGTCACAAACCTG	AGCAAGGCGTACAAAACAAC
3863320	GTTGCTCACAATACTGGAGG	TGTCTTCAGCAGAACTCATC
1373432	TAAGGCAGAGGGCTACTTAC	GCATTTTACAAGACTTAAATC
		G
1047699	ATTACCAGAGTGGTTGCTCC	ATTTCCTTCTGTCAGCCTGC
1063193	AGGCTGACAGAAGGAAATCG	TCAGCAGAGACCACCAAAAC
2232959	TCAGCAGAGACCACCAAAAC	GGTGGTCGTTGCTGATTTTG
2227296	TGAGAAGAAGACACTGAAGG	ATCCCAATGATCCTCAATCC
1548804	CCCATTTTTCTTCCAATCAC	GGAGCAACCAGGAGTAAATA
2232958	TCTTCTCAACCTCCTTCCTC	ATGACTCTTCTAGGGCCTCA
2232957	TTTATAAACATGAGCAGAAC	CGCATTCAACTTTATGAGAGG
2232956	GCCAATTTTGCCTTACTAACC	CTAGCATTAACCAGAGATAC
1972571	ATCACCAGCTGCCACTTTTC	CCTCAAATTATCACCAGCCC
3759250	TTCTGCCTAGGTGGCTTATG	AATCATGTCTGCATGGCACG
3759249	CACACAGTGAATCATGTCTG	ATGGTAGACCATCAGGTAGG
1541525	TATCTGTAGACGTGCATCCC	CTGACACCTGTCCTCATAAG
2098248	GGGAGAATCATGGCTTGA	GTACTGCCCTTAGCAAATCC
2900550	TCAACAGTCTCCAGCTGATC	ACTCTGGCTGTTGTAAGGAG
7302130	TACACCTGTGAGCACTGACA	CCTTTTCTCAAGGTGTGTGC
4763583	AATGGTTTAGCACCATCCCC	GGGAGGTGACACACACTTTT
4360778	AGGCTGCAAGTTTTCCAAAC	GCCTAAGCAAAGAAATGAGC
1607695	TTCTTCTCACTGACCTATTC	ATCAAACAGAAACGCTGGAG
1607694	ATCAAACAGAAACGCTGGAG	CTCTTTTTTTGTCTGACTGGG
2192139	GGTCTTTTGTTTGCTAGGAC	GTAACCTGCCAAGATTGAAC
7978300	TCTGAGTCTTTTGTGGTACC	TGCAGATTCAATGCAATCCC
7397871	AGGAGCTGAAACGATGGTTC	CAAAATGGCATGACACTGGC
4763217	ACATTGTGTCTGTGCTTCTC	GCAGTCTCTTATTGTCTGAG
2159900	TTACAACAAGTGCTCAAGGG	GTGTCTTCTCTAACAGTGAG
10772370	GAATGACCATATGATCCAGC	TAGGTATCCCTTTGATGTCC
7398682	GACAGATACCCTAACTTGATC	GCAGTAAAGCTATCAATCCTG
2900551	GTACCTCAACACAAAAAGGC	GAGGAAATCTTTCACCTTTCC
2900552	GCTAGATATACTAGGCAAGAG	TGTCATCCGAAAAGATGAAC
2418214	TGTCATCCGAAAAGATGAAC	GCTAGATATACTAGGCAAGAG
2418215	CTATTCTGCAACTTTACCAG	TAGAAGGAAGTCAATTGTTC
965243	GGCTGTGTAGGTTTGTCCTC	TTCATCCCATTCCATCCAGC
1117548	GTGGTACATCACATTAACAG	TTCCTTCTTTGTGTAGTTCG
1520225	CGTGACTCTCTGTACAGCAT	CAGCACTAGGCTGTGAAAAG
1520226	TAGGGTAAAATGTGCACAGC	GCATTGTTAGTGGTTGCTCC
1520227	TAGGGTAAAATGTGCACAGC	GCATTGTTAGTGGTTGCTCC
971919	TTAGTGACCTTCATAGAACC	CAGGCATTCTTAGAAGACAG
2159901	CATATCTACTTGTGAACTGC	GAAGGTAAAACCACTGATGC
2159902	CCCCTATAAGAGCAAAATAC	GAACTTTTCAGAAAGCATCAG
2110099	ATTCCTTCTCAACCCACATC	ACATTACAGGAAGGCCTTTC
7314847	CCTCTGCATAGATACAGTAG	CTCTTATACACCCTGATTGG
7296003	TGGTTACTTAGGTTGATGCC	CTACTGTATCTATGCAGAGG
4281556	GGCCTTAGGGTTTCCATAAC	AAGCCTTGGAAGAGTGAGTG
4763219	AGGAGAATCGCTTGAACCTG	TGGAGTCTCATTCTGTCACC
3851579	AGGCTTTCAAACAAAATAGG	TTACGGGTCTGATAAGAAAG
3851580	AGGCTTTCAAACAAAATAGG	TTACGGGTCTGATAAGAAAG
1049119	GATAACAGTGTTTCAAATGC	GAAATTGCAAGCTGATTGTT
2298866	TAACCAATCCCTGTCACTGG	ACAAAGATGGGCACTGCAAC
2298865	CCTGAGTTCATTTAGATCTC	ACGGAACAACCAAACAATAG
2298864	CCAGTAAACACTGAAGAGATC	GATTTGTGTCTTACTCACTGG
2298863	AGCTCTTGAAGGCAATTCTG	TATGTCCTCATTGTCAACCC
3180393	AACAAGGTCCACCACCTCCT	TTGTGGCCTTCCTTGAGGAG
2070837	ACACACCCACACAAACTCAC	TGGAAAGACTGCTATTCTGC
7956204	TTTGGACAATTCTTGTAGCC	TGTGAGATGTGTGAGGACAG
2418216	CCCAGGCACACATATATGAG	GCCTGAGTGTAGTGAGATTC
3741844	GCTGATTGCTCTGTGATACC	AAGTGCAGCTGGTGATTCTG
4262798	TCTAGGTACCCAAGCTCCTG	AAGAGCTGAAAGGGACACTG
2418217	GCCTGGAACACTAAAGATGG	CTCACTGACTCTCAGAGAAG
2418218	AAATTAGTCAGTCATGGTGG	TTCAAGCAATTCTCCTGCCT
7137492	TCCTCATTTACTACAGTGAC	CCCATCTCTACTAAAAATAC
2110100	CTGGAATTACAAAGAGAAGAG	CCCATCTCTACTAAAAATAC
1013312	ATTGTGTTTGCCCACTTTCC	GTGGAACATCAAGAATGAAG
4579993	ATTTGTGCTCCTTTCTACTG	AGCAGAAAGAAGGAAATGAC
1013313	TTCCAAACACAGCAAAGAGC	TTGGTTGTATTCTGGTTGGG
7397106	TCTGAAACTCAGAATGCATG	ATACTCTAAACAATACAGGG
2215716	GTACTAGCAGTAGTCAGAGG	GACACCACTACTTGCACATG
2192140	CCTTGGGATTTAGAAATAGGG	GTAAGCAAATATCTCTGGAG
4763589	TTGGCAACTGTGTAACCTTG	TCAAACATACTGTTTGCTCAC
1468697	GAACACAATCGCAAGTTTAAG	GTTCTTCAAAATCTGCTTCC
2070837	ACACACCCACACAAACTCAC	TGGAAAGACTGCTATTCTGC
3180393	AACAAGGTCCACCACCTCCT	TTGTGGCCTTCCTTGAGGAG
2298865	CCTGAGTTCATTTAGATCTC	ACGGAACAACCAAACAATAG

TABLE 14
dbSNP	Extend	Term
rs#	Primer	Mix
523051	TCAGAGCATAGATGATGGCAA	ACT
693620	AGAGGAGGAAACCTTAATTTCT	ACT
2588349	AGAGTTTGATGTCAAGGAAATG	ACG
2588350	TACCATCAAAAGCACATCATTC	ACT
619381	GAATTTTCTTCCTTTTAGAATAGA	ACG
3759252	TGGTGAGTCCATAGCTCTAAT	CGT
3759251	CCAGCTACTTTATGCCAGAG	CGT
2418107	TGGCCAGGCCAAGGATCG	ACT
7303054	CAGCCTAAATTAACCGTATGTG	ACT
1838345	CCTGCCTGAGCCTGCCAA	ACT
620878	TTCAATAATATTATAGTGAGGATG	ACT
2537817	GACATGTAGAAAAATTGCCTGC	ACT
1548803	TTGCAAACAGGTTAAAGAGAGT	ACG
667123	AAGCCCTAGGGTATTGTGATT	ACG
1838346	AAGGCTAGAGAGAACATTCCA	ACT
2159903	AGGGGTCTCTATGGAACAAAA	ACT
3944035	AACAAAAGGCTTTCTTCTTCTAA	ACT
3741845	CAAAGGATAAAGGGAACCATC	ACT
2110096	CAGGATACTGCGATACTGTC	ACG
759055	GTAATCTTGTTTGTTGTATTTTTC	CGT
589377	CTGGTGTGTAGAAACACAACA	ACT
7960194	GCCCAGGGCTCAGGGAAG	CGT
7978242	GCAAACCAAAACCACAGTCAC	ACT
601051	CCCGCTTATAAGCAAGAGCA	ACG
4262797	TTATCCCTATCCTCCCTTCC	ACT
2215714	GCATCACTCTTTCTACGCAATA	ACT
1373434	TGTTTCAAAACATCACTATGTAC	ACG
2215715	GGAAAGGAAGACATTATGGTAC	ACG
612456	CCAAGATCCCTGAAAGGCAAA	CGT
612808	CTACTTTAGCCTTTTGTTGGAC	ACT
689118	GCTTCATACACACACACACAT	ACT
597468	CCCAGACTGTGTCATTCTTC	ACT
592864	GTGTTTCTTGTAAGCAGTATAC	ACT
640372	AAACTCTTTGAGCGCTGACAT	ACT
7966559	TCTATCGGTGATTTCTGCTGT	CGT
654834	CACAATTGGCTTTTAAAACTCC	ACT
4763216	CCATAGAAAGCAGGACTGGTT	ACT
668521	AAATTAGTCTAGAACTTGTGGAA	ACT
669503	CCACTCATTCAGATCTACAGAA	ACT
3906864	ACATCCACCTTCTGTAGATCT	ACT
3906863	GTGTGAATAAGTTGTAATCCAC	ACG
7957888	TGTGGAAATATTTGAAGACTCTT	CGT
9300230	ATACAGAGAAGTAGAGGACAAA	CGT
7306214	CACAGTAGGGTGACTACAATTA	ACT
763839	TAACACCGAAGGTTCCTCAG	ACT
2418105	CATTCTTTTCCAGTGTCAATCAA	ACG
666841	GGACTTGGCAGCATTATTTATTA	ACG
3851578	CAGCTAATTGTGCTCCCTCA	ACT
7138797	AAGTACATGTGCAGAATGTGC	ACT
7295252	ATCACAGCTGATAATGTCATAAT	ACT
2418106	GAATGGCTTTGAATATGACCCA	ACT
7299578	CAGGTGCTATCACAACATCG	ACT
621112	CACAAACCTGCCATTTTACTTT	ACT
3863320	AATACTGGAGGCTGGAAGAC	ACG
1373432	CAGAGGGCTACTTACAAGAATT	CGT
1047699	TCTTGCTGGTCTGTCCCTC	ACG
1063193	AGTTGACGGTGTCCTCGT	ACG
2232959	ATCACCGCCATCCTCCCC	ACT
2227296	GAAATTCTAGTGGAAAAAG	ACT
1548804	AAAATTTCAATATGTTGCAGGCAG	ACG
2232958	CCTCCCCTCCTGCTCTTC	ACG
2232957	GGACCTCACCACCTGAAAG	ACT
2232956	TGCCTTACTAACCTCCAGG	ACT
1972571	CTGCCACTTTTCATTACAGGC	ACT
3759250	TAGGTGGCTTATGGTAGACC	ACT
3759249	TCTGCATGGCACGCCCCT	ACT
1541525	CCCTCTGTGGTCTGTGCTAA	ACG
2098248	GAATCATGGCTTGACTCAGG	ACT
2900550	GCCCCTCACCACATCCCA	CGT
7302130	CCCACCCAGCAGGAAGAC	CGT
4763583	CCTGCGTACTATTCTTATGAC	ACT
4360778	TTTTACACTCTGCTTCCCTTTT	CGT
1607695	TTCTCACTGACCTATTCTATTTT	ACG
1607694	AGCTTCAAAAATAGAATAGGTCA	ACT
2192139	CTTTGATCTCATCACTTGTTATT	ACT
7978300	TTCTGTTTCTGTGAAGAATGTC	ACT
7397871	GCCAGGCTCACAGTCCAAA	CGT
4763217	TTGGACGTAGGACAAAGAACT	ACG
2159900	GCTCAAGGGAGTTCTACATCT	ACT
10772370	GACCATATGATCCAGCAATTTC	ACT
7398682	GCTTATAACAAAATATCATGTGTC	ACG
2900551	GCCATATATGACAAACCCACA	ACT
2900552	ATATACTAGGCAAGAGATAGAAA	ACG
2418214	CTTCCTTCTATTCAATTTCAATTT	ACT
2418215	CTATAAATAAGATCATGTCATCC	ACT
965243	CAGACCCATCAGAGAAGTAC	CGT
1117548	CCAAATAAGCATATGAATAGATG	CGT
1520225	CAGCATTGTTAGTGGTTGCTC	ACG
1520226	GCTGTGAAAAGCTATGTATGC	ACT
1520227	CAGCACTAGGCTGTGAAAAG	ACT
971919	ATAAGATAGGTCTGTTGGAAAG	CGT
2159901	TGATTTCTTCAGCCATTATCCA	ACG
2159902	TTAAGCTTGCATGAAAGAAGGT	ACT
2110099	TTATGTCAGCAGAAAAACAGAAA	ACT
7314847	GAGTTTTAGTTCTTTGAGAAATC	ACT
7296003	GGTTGATGCCATATCTTTGCT	ACT
4281556	GGTTTCCATAACAAGAATAACAAA	ACG
4763219	CCGATATCTTGCCACTGCAG	ACT
3851579	CAAAATAGGAACACACTGAGTAT	ACG
3851580	CTTTCAAACAAAATAGGAACACA	ACG
1049119	TGGCATCATGCTCTAACTTCA	ACT
2298866	CCTGTCACTGGATATTAAGGC	ACT
2298865	CATTTAGATCTCTTCAGTGTTTA	ACG
2298864	CTGAAGAGATCTAAATGAACTCA	ACT
2298863	CAATTCTGATTTTGAGAATCACT	ACT
3180393	GCCACAAGGACCACCCCA	ACT
2070837	CCACACAAACTCACATACACA	ACT
7956204	AGGCTGCCAGCACCTTTCT	ACG
2418216	GCACACATATATGAGAGAAAGG	ACT
3741844	GTGATACCTAGAAATTCCCTG	ACG
4262798	CGTCTCCCTTTCACCCACC	ACG
2418217	TGCCAGGAAAGATGAACATTG	ACT
2418218	CAGTCATGGTGGCGAGGG	ACT
7137492	GCCATGTGACTTAATTTCTTTAG	ACT
2110100	TCCTCATTTACTACAGTGACC	ACG
1013312	GCCCACTTTCCCCCTTTCT	ACT
4579993	ACTGTTATTTCTGGTTTCTGGT	ACT
1013313	ACAGCAAAGAGCATAAACCTAC	ACT
7397106	CTCAGAATGCATGAATAGTACA	ACG
2215716	CGTACTATTTCTTCAAGCTTCA	ACT
2192140	TAGGGTATCCCTCTTGGTCA	ACT
4763589	CTGTGTAACCTTGATCTTGGG	ACT
1468697	CAATCGCAAGTTTAAGGTAACA	ACT
2070837	CCACACAAACTCACATACACA	ACT
3180393	GCCACAAGGACCACCCCA	ACT
2298865	CATTTAGATCTCTTCAGTGTTTA	ACG

Genetic Assay

Allelotyping results are shown for female cases and controls in Table 15. The allele frequency for A2 allele is noted in the fifth and sixth columns for control pools and case pools, respectively, where “AF” is allele frequency. Some SNPs do not have an allele frequency disclosed because of failed assays.

TABLE 15
Female Allelotyping Results
	Position in							Low BMD
dbSNP	SEQ ID	Chromosome	A1/A2	Controls (high	Cases (Low			Associated
rs#	NO: 2	Position	Allele	BMD) AF	BMD) AF	p-Value	OR	Allele
523051	229	10842129	T/C	T = 0.00	T = 0.00
				C = 1.00	C = 1.00
693620	368	10842268	T/C	T = 1.00	T = 1.00
				C = 0.00	C = 0.00
2588349	583	10842483	G/A	G = 1.00	G = 1.00
				A = 0.00	A = 0.00
2588350	2424	10844324	T/C	T = 0.34	T = 0.28	0.0295	0.76	C
				C = 0.66	C = 0.72
619381	3625	10845525	C/T	C = 0.86	C = 0.91	0.0065	1.67	C
				T = 0.14	T = 0.09
3759252	3709	10845609	C/A	C = 1.00	C = 1.00
				A = 0.00	A = 0.00
3759251	3750	10845650	T/A	T = 0.01	T = 0.01
				A = 0.99	A = 0.99
2418107	4724	10846624	G/C	G = 1.00	G = 1.00
				C = 0.00	C = 0.00
7303054	4781	10846681	T/C	T = 0.00	T = 0.00
				C = 1.00	C = 1.00
1838345	6161	10848061	A/G	A =	A =
				G =	G =
620878	7097	10848997	T/G	T = 0.07	T = 0.03	0.0098	0.37	G
				G = 0.93	G = 0.97
2537817	8025	10849925	T/C	T = 0.19	T = 0.16	0.4485	0.85	C
				C = 0.81	C = 0.84
1548803	8398	10850298	C/T	C = 0.47	C = 0.41	0.0564	0.78	T
				T = 0.53	T = 0.59
667123	10144	10852044	G/A	G = 1.00	G = 1.00
				A = 0.00	A = 0.00
1838346	10384	10852284	A/G	A = 0.76	A = 0.80	0.1329	1.26	A
				G = 0.24	G = 0.20
2159903	11116	10853016	A/G	A = 0.00	A = 0.00
				G = 1.00	G = 1.00
3944035	11132	10853032	A/G	A = 0.00	A = 0.00
				G = 1.00	G = 1.00
3741845	11482	10853382	T/C	T = 0.54	T = 0.49	0.0877	0.81	C
				C = 0.46	C = 0.51
2110096	14544	10856444	C/T	C = 1.00	C = 1.00
				T = 0.00	T = 0.00
759055	15688	10857588	T/A	T = 0.45	T = 0.37	0.0084	0.72	A
				A = 0.55	A = 0.63
589377	17311	10859211	G/C	G = 0.01	G = 0.00
				C = 0.99	C = 1.00
7960194	17831	10859731	G/T	G = 1.00	G = 1.00
				T = 0.00	T = 0.00
7978242	20012	10861912	A/G	A = 0.62	A = 0.68	0.0611	1.27	A
				G = 0.38	G = 0.32
601051	21997	10863897	G/A	G = 0.01	G = 0.00
				A = 0.99	A = 1.00
4262797	22861	10864761	A/G	A = 0.61	A = 0.54	0.0391	0.76	G
				G = 0.39	G = 0.46
2215714	23470	10865370	A/G	A = 0.00	A = 0.00
				G = 1.00	G = 1.00
1373434	23515	10865415	G/A	G = 1.00	G = 1.00
				A = 0.00	A = 0.00
2215715	23863	10865763	G/A	G = 1.00	G = 1.00
				A = 0.00	A = 0.00
612456	24108	10866008	C/A	C = 0.03	C = 0.02
				A = 0.97	A = 0.98
612808	24138	10866038	T/C	T = 0.20	T = 0.11	0.0001	0.52	C
				C = 0.80	C = 0.89
689118	26469	10868369	T/C	T = 0.68	T = 0.71	0.3485	1.15	T
				C = 0.32	C = 0.29
597468	27769	10869669	A/G	A = 0.99	A = 1.00
				G = 0.01	G = 0.00
592864	29683	10871583	T/C	T = 0.10	T = 0.11	0.9292	1.04	T
				C = 0.90	C = 0.89
640372	30491	10872391	A/C	A = 0.96	A = 0.96	0.6780	1.21	A
				C = 0.04	C = 0.04
7966559	30745	10872645	A/T	A = 0.79	A =
				T = 0.21	T =
654834	31429	10873329	T/C	T = 0.04	T = 0.03	0.3922	0.74	C
				C = 0.96	C = 0.97
4763216	31779	10873679	C/G	C = 0.50	C = 0.45	0.0951	0.82	G
				G = 0.50	G = 0.55
668521	32194	10874094	A/C	A = 0.98	A = 0.99
				C = 0.02	C = 0.01
669503	32441	10874341	T/C	T = 0.96	T = 0.96	0.4685	1.26	T
				C = 0.04	C = 0.04
3906864	32454	10874354	T/C	T = 0.99	T = 1.00
				C = 0.01	C = 0.00
3906863	32459	10874359	C/T	C = 0.34	C = 0.41	0.0288	1.35	C
				T = 0.66	T = 0.59
7957888	35151	10877051	A/T	A = 0.87	A = 0.93	0.0155	2.12	A
				T = 0.13	T = 0.07
9300230	35362	10877262	A/T	A = 1.00	A = 1.00
				T = 0.00	T = 0.00
7306214	35630	10877530	G/C	G = 1.00	G = 1.00
				C = 0.00	C = 0.00
763839	36930	10878830	G/C	G = 0.82	G = 0.88	0.0047	1.61	G
				C = 0.18	C = 0.12
2418105	37490	10879390	G/A	G = 0.21	G = 0.16	0.0198	0.69	A
				A = 0.79	A = 0.84
666841	38432	10880332	C/T	C = 0.89	C = 0.93	0.0180	1.72	C
				T = 0.11	T = 0.07
3851578	38688	10880588	A/G	A = 0.89	A = 0.93	0.0243	1.67	A
				G = 0.11	G = 0.07
7138797	39524	10881424	T/C	T = 0.97	T = 0.97	0.9815	0.99	T
				C = 0.03	C = 0.03
7295252	41580	10883480	A/C	A = 1.00	A = 1.00
				C = 0.00	C = 0.00
2418106	42531	10884431	T/C	T = 0.35	T = 0.30	0.1784	0.80	C
				C = 0.65	C = 0.70
7299578	42665	10884565	A/G	A = 0.30	A = 0.23	0.0036	0.67	G
				G = 0.70	G = 0.77
621112	43038	10884938	A/G	A = 0.87	A = 0.92	0.0086	1.69	A
				G = 0.13	G = 0.08
3863320	44183	10886083	G/A	G = 0.52	G = 0.49	0.2271	0.87	A
				A = 0.48	A = 0.51
1373432	46271	10888171	A/T	A = 0.00	A = 0.00
				T = 1.00	T = 1.00
1047699	49075	10890975	C/T	C = 0.79	C = 0.85	0.0062	1.53	C
				T = 0.21	T = 0.15
1063193	49147	10891047	C/T	C = 0.75	C =
				T = 0.25	T =
2232959	49180	10891080	C/G	C = 1.00	C = 1.00
				G = 0.00	G = 0.00
2227296	50301	10892201	A/G	A = 0.77	A = 0.77	0.9934	1.00	A
				G = 0.23	G = 0.23
1548804	50773	10892673	G/A	G = 0.24	G = 0.15	0.0005	0.54	A
				A = 0.76	A = 0.85
2232958	51243	10893143	G/A	G = 1.00	G = 1.00
				A = 0.00	A = 0.00
2232957	51530	10893430	T/C	T = 1.00	T = 1.00
				C = 0.00	C = 0.00
2232956	52107	10894007	A/G	A = 0.87	A = 0.92	0.0170	1.64	A
				G = 0.13	G = 0.08
1972571	52821	10894721	A/G	A = 0.13	A = 0.13	0.8117	1.04	A
				G = 0.87	G = 0.87
3759250	53341	10895241	T/C	T = 0.99	T = 1.00
				C = 0.01	C = 0.00
3759249	53376	10895276	C/G	C = 1.00	C = 1.00
				G = 0.00	G = 0.00
1541525	54047	10895947	C/T	C = 0.00	C = 0.00
				T = 1.00	T = 1.00
2098248	54392	10896292	T/C	T = 0.00	T = 0.00
				C = 1.00	C = 1.00
2900550	54482	10896382	T/A	T = 0.89	T = 0.93	0.0535	1.59	T
				A = 0.11	A = 0.07
7302130	55468	10897368	C/A	C = 0.36	C = 0.36	0.9550	1.01	C
				A = 0.64	A = 0.64
4763583	56990	10898890	A/G	A =	A =
				G =	G =
4360778	57712	10899612	A/T	A = 0.00	A = 0.01
				T = 1.00	T = 0.99
1607695	59667	10901567	C/T	C = 0.00	C = 0.01
				T = 1.00	T = 0.99
1607694	59684	10901584	T/C	T = 0.81	T = 0.83	0.3469	1.17	T
				C = 0.19	C = 0.17
2192139	62043	10903943	A/C	A = 0.30	A = 0.28	0.4555	0.89	C
				C = 0.70	C = 0.72
7978300	63293	10905193	T/C	T = 0.36	T =
				C = 0.64	C =
7397871	63485	10905385	G/T	G = 0.00	G = 0.00
				T = 1.00	T = 1.00
4763217	63778	10905678	C/T	C = 0.89	C = 0.90	0.8698	1.05	C
				T = 0.11	T = 0.10
2159900	64222	10906122	A/G	A = 0.02	A = 0.02
				G = 0.98	G = 0.98
10772370	65722	10907622	A/G	A = 0.31	A = 0.27	0.1478	0.82	G
				G = 0.69	G = 0.73
7398682	66315	10908215	C/T	C = 1.00	C = 1.00
				T = 0.00	T = 0.00
2900551	66829	10908729	A/G	A = 0.00	A = 0.01
				G = 1.00	G = 0.99
2900552	66966	10908866	C/T	C = 0.61	C = 0.59	0.5150	0.92	T
				T = 0.39	T = 0.41
2418214	66971	10908871	A/C	A = 0.51	A = 0.57	0.0565	1.27	A
				C = 0.49	C = 0.43
2418215	67013	10908913	T/C	T = 0.82	T = 0.86	0.1232	1.29	T
				C = 0.18	C = 0.14
965243	70375	10912275	A/T	A = 0.82	A = 0.85	0.1637	1.25	A
				T = 0.18	T = 0.15
1117548	74118	10916018	T/A	T = 1.00	T = 0.99
				A = 0.00	A = 0.01
1520225	75224	10917124	C/T	C = 0.27	C = 0.22	0.0956	0.79	T
				T = 0.73	T = 0.78
1520226	75236	10917136	T/C	T = 0.99	T = 0.98
				C = 0.01	C = 0.02
1520227	75246	10917146	G/C	G = 0.91	G = 0.82	0.00001	0.46	C
				C = 0.09	C = 0.18
971919	75812	10917712	C/A	C = 0.00	C = 0.00
				A = 1.00	A = 1.00
2159901	78968	10920868	C/T	C = 0.99	C = 0.99
				T = 0.01	T = 0.01
2159902	78998	10920898	T/C	T = 0.54	T = 0.51	0.3599	0.90	C
				C = 0.46	C = 0.49
2110099	79328	10921228	A/C	A = 0.60	A = 0.63	0.4080	1.11	A
				C = 0.40	C = 0.37
7314847	80922	10922822	T/C	T = 0.51	T = 0.49	0.6059	0.94	C
				C = 0.49	C = 0.51
7296003	81055	10922955	T/C	T = 0.63	T = 0.65	0.4799	1.09	T
				C = 0.37	C = 0.35
4281556	81412	10923312	G/A	G = 0.85	G = 0.87	0.5327	1.17	G
				A = 0.15	A = 0.13
4763219	81785	10923685	A/G	A =	A =
				G =	G =
3851579	82079	10923979	G/A	G =	G = 0.34
				A =	A = 0.66
3851580	82087	10923987	G/A	G = 0.56	G = 0.54	0.5618	0.93	A
				A = 0.44	A = 0.46
1049119	82958	10924858	A/G	A = 0.00	A = 0.00
				G = 1.00	G = 1.00
2298866	83351	10925251	T/G	T = 0.72	T = 0.69	0.3240	0.87	G
				G = 0.28	G = 0.31
2298865	83442	10925342	C/T	C = 0.95	C = 0.97	0.1804	1.67	C
				T = 0.05	T = 0.03
2298864	83472	10925372	A/C	A = 0.36	A = 0.34	0.5936	0.93	C
				C = 0.64	C = 0.66
2298863	83966	10925866	A/G	A = 1.00	A = 1.00
				G = 0.00	G = 0.00
3180393	84414	10926314	T/C	T = 1.00	T = 1.00
				C = 0.00	C = 0.00
2070837	86563	10928463	C/G	C = 0.43	C = 0.43	0.9403	0.99	C
				G = 0.57	G = 0.57
7956204	86788	10928688	G/A	G = 0.86	G =
				A = 0.14	A =
2418216	86796	10928696	T/G	T = 1.00	T = 1.00
				G = 0.00	G = 0.00
3741844	87634	10929534	G/A	G = 0.50	G = 0.48	0.3827	0.90	A
				A = 0.50	A = 0.52
4262798	88530	10930430	G/A	G = 0.60	G = 0.62	0.4130	1.10	G
				A = 0.40	A = 0.38
2418217	89202	10931102	A/G	A = 0.53	A = 0.52	0.6562	0.95	G
				G = 0.47	G = 0.48
2418218	89632	10931532	T/C	T =	T =
				C =	C =
7137492	89697	10931597	T/C	T = 0.75	T = 0.76	0.7043	1.07	T
				C = 0.25	C = 0.24
2110100	89723	10931623	G/A	G = 0.53	G = 0.52	0.5403	0.93	A
				A = 0.47	A = 0.48
1013312	91063	10932963	C/G	C = 0.29	C = 0.26	0.1928	0.84	G
				G = 0.71	G = 0.74
4579993	91335	10933235	C/G	C = 0.00	C = 0.00
				G = 1.00	G = 1.00
1013313	91504	10933404	T/C	T = 0.61	T = 0.58	0.3893	0.90	C
				C = 0.39	C = 0.42
7397106	91619	10933519	C/T	C = 0.02	C = 0.04
				T = 0.98	T = 0.96
2215716	93715	10935615	A/G	A = 0.45	A = 0.36	0.0372	0.69	G
				G = 0.55	G = 0.64
2192140	93945	10935845	T/C	T = 0.00	T = 0.00
				C = 1.00	C = 1.00
4763589	94235	10936135	T/C	T = 0.53	T = 0.52	0.6806	0.95	C
				C = 0.47	C = 0.48
1468697	95851	10937751	T/C	T = 0.02	T = 0.01
				C = 0.98	C = 0.99
2070837	130860	10972760	C/G	C = 0.43	C = 0.43	0.9403	0.99	C
				G = 0.57	G = 0.57
3180393	132815	10974715	T/C	T = 1.00	T = 1.00
				C = 0.00	C = 0.00
2298865	133778	10975678	C/T	C = 0.95	C = 0.97	0.1804	1.67	C
				T = 0.05	T = 0.03

Allelotyping results were considered particularly significant with a calculated p-value of less than or equal to 0.05 for allelotype results. These values are indicated in bold. The allelotyping p-values were plotted in FIG. 2. The position of each SNP on the chromosome is presented on the x-axis. The y-axis gives the negative logarithm (base 10) of the p-value comparing the estimated allele in the case group to that of the control group. The minor allele frequency of the control group for each SNP designated by an X or other symbol on the graphs in FIG. 2 can be determined by consulting Table 15. For example, the left-most X on the left graph is at position 10842129. By proceeding down the Table from top to bottom and across the graphs from left to right the allele frequency associated with each symbol shown can be determined.

To aid the interpretation, multiple lines have been added to the graph. The broken horizontal lines are drawn at two common significance levels, 0.05 and 0.01. The vertical broken lines are drawn every 20 kb to assist in the interpretation of distances between SNPs. Two other lines are drawn to expose linear trends in the association of SNPs to the disease. The light gray line (or generally bottom-most curve) is a nonlinear smoother through the data points on the graph using a local polynomial regression method (W. S. Cleveland, E. Grosse and W. M. Shyu (1992) Local regression models. Chapter 8 of Statistical Models in S eds J. M. Chambers and T. J. Hastie, Wadsworth & Brooks/Cole.). The black line provides a local test for excess statistical significance to identify regions of association. This was created by use of a 10 kb sliding window with 1 kb step sizes. Within each window, a chi-square goodness of fit test was applied to compare the proportion of SNPs that were significant at a test wise level of 0.01, to the proportion that would be expected by chance alone (0.05 for the methods used here). Resulting p-values that were less than 10⁻⁸ were truncated at that value.

Finally, the exons and introns of the genes in the covered region are plotted below each graph at the appropriate chromosomal positions. The gene boundary is indicated by the broken horizontal line. The exon positions are shown as thick, unbroken bars. An arrow is place at the 3′ end of each gene to show the direction of transcription.

Example 5

GRID2 Proximal SNPs

It has been discovered that a polymorphic variation (rs1948017) in a gene encoding GRID2 is associated with the occurrence of low BMD (see Examples 1 and 2). One hundred five additional allelic variants proximal to rs1948017 were identified and subsequently allelotyped in low BMD case and high BMD control sample sets as described in Examples 1 and 2. The polymorphic variants are set forth in Table 16. The chromosome position provided in column four of Table 16 is based on Genome “Build 34” of NCBI's GenBank.

TABLE 16
	Position in
	SEQ ID		Chromosome
dbSNP	NO: 3	Chromosome	Position	Alleles (A1/A2)	genome_letter	deduced_iupac
1433661	206	4	94744306	t/c	g	R
1485009	243	4	94744343	a/g	g	R
7681947	2467	4	94746567	t/c	t	Y
1816432	3550	4	94747650	a/g	t	Y
1485018	4994	4	94749094	c/g	g	S
1485017	5167	4	94749267	g/t	c	M
7438397	5193	4	94749293	c/t	c	Y
6834311	5273	4	94749373	g/a	g	R
1368717	5733	4	94749833	a/g	c	Y
1017391	7817	4	94751917	a/c	g	K
2870701	7818	4	94751918	t/a	a	W
7679839	8612	4	94752712	t/g	t	K
1385404	9158	4	94753258	a/g	a	R
1368716	9285	4	94753385	g/a	c	Y
4693316	10680	4	94754780	g/a	a	R
1905707	11866	4	94755966	t/c	c	Y
1905708	11958	4	94756058	a/g	a	R
1905709	12044	4	94756144	a/t	t	W
3912442	12753	4	94756853	g/t	t	K
2082553	15585	4	94759685	a/g	c	Y
6831638	17299	4	94761399	c/t	c	Y
5860329	18816	4	94762916	—/g	g	N
2870702	24022	4	94768122	c/t	t	Y
2870703	24994	4	94769094	c/t	t	Y
1948016	26637	4	94770737	g/t	t	K
6835836	27635	4	94771735	c/g	g	S
1994253	28773	4	94772873	t/c	t	Y
1905710	29430	4	94773530	a/t	a	W
1485019	29876	4	94773976	t/a	t	W
978191	30364	4	94774464	c/t	a	R
1385405	31057	4	94775157	c/a	t	K
7694361	31782	4	94775882	c/t	t	Y
1905711	33400	4	94777500	a/c	c	M
1905734	35588	4	94779688	a/c	t	K
1485012	37663	4	94781763	c/g	c	S
1485013	37865	4	94781965	t/c	c	Y
4692981	38218	4	94782318	a/t	a	W
7670552	39375	4	94783475	t/c	c	Y
7670932	39559	4	94783659	c/t	c	Y
7688091	39833	4	94783933	a/g	g	R
7440540	40135	4	94784235	a/g	g	R
2171000	41698	4	94785798	g/a	t	Y
2870704	42249	4	94786349	t/c	t	Y
7655758	42571	4	94786671	g/c	c	S
7661436	42977	4	94787077	g/a	g	R
7662289	43548	4	94787648	t/c	c	Y
7667044	43631	4	94787731	g/a	a	R
7691929	43705	4	94787805	t/c	t	Y
5860330	43817	4	94787917	tt/t—	t—	N
901013	44374	4	94788474	a/c	g	K
901012	44464	4	94788564	a/c	g	K
901011	44788	4	94788888	t/c	g	R
1948018	48962	4	94793062	a/g	c	Y
2870705	48993	4	94793093	t/g	t	K
1948017	49110	4	94793210	c/t	a	R
1905733	49434	4	94793534	c/t	a	R
1385408	49523	4	94793623	t/g	t	K
1385409	49742	4	94793842	c/g	c	S
1385410	49907	4	94794007	g/c	g	S
1485026	50028	4	94794128	g/c	g	S
1485027	50089	4	94794189	g/t	t	K
2904483	51588	4	94795688	c/a	a	M
1385406	52899	4	94796999	c/a	a	M
1905732	54088	4	94798188	a/c	t	K
2046418	56538	4	94800638	c/t	a	R
2200377	59071	4	94803171	t/c	g	R
1905731	59110	4	94803210	a/c	t	K
1905730	59178	4	94803278	a/g	c	Y
975713	61087	4	94805187	t/c	a	R
6820985	61300	4	94805400	c/g	c	S
7670441	62171	4	94806271	t/c	c	Y
6810794	62783	4	94806883	t/a	t	W
7676623	62983	4	94807083	t/c	c	Y
1154861	63908	4	94808008	c/t	t	Y
1032125	64088	4	94808188	g/t	g	K
1485022	64941	4	94809041	a/t	a	W
1485024	65050	4	94809150	a/g	g	R
3913651	68953	4	94813053	c/t	c	Y
4693319	70093	4	94814193	t/c	c	Y
1872383	71308	4	94815408	c/a	g	K
2200376	73009	4	94817109	t/a	t	W
7668090	74002	4	94818102	a/g	g	R
7692930	74294	4	94818394	t/c	t	Y
967096	74879	4	94818979	g/c	g	S
6822249	76936	4	94821036	g/t	t	K
6532405	77195	4	94821295	g/a	g	R
1017897	77683	4	94821783	t/c	c	Y
7672674	78283	4	94822383	t/c	t	Y
7694568	78331	4	94822431	c/t	c	Y
2904484	79362	4	94823462	g/c	c	S
7340830	80357	4	94824457	c/t	c	Y
1485033	80653	4	94824753	t/c	g	R
2870706	80840	4	94824940	a/g	g	R
1905729	83203	4	94827303	a/g	c	Y
4693320	85405	4	94829505	t/c	t	Y
6848749	86441	4	94830541	g/t	t	K
6532406	86967	4	94831067	g/a	g	R
6532407	87121	4	94831221	t/c	t	Y
1905728	89617	4	94833717	t/c	g	R
6819866	90969	4	94835069	t/a	a	W
1905727	94249	4	94838349	g/c	g	S
7674069	95811	4	94839911	t/g	t	K
1905724	96690	4	94840790	t/g	c	M
1905723	96731	4	94840831	a/g	c	Y
1485020	97267	4	94841367	c/g	c	S
6814101	97414	4	94841514	t/g	g	K

Assay for Verifying and Allelotyping SNPs

the methods used to verify and allelotype the proximal SNPs of Table 16 are the same methods described in Examples 1 and 2 herein. The primers and probes used in these assays are provided in Table 17 and Table 18, respectively.

TABLE 17
DbSNP
rs#	Forward PCR primer	Reverse PCR primer
1433661	CTCCCAAAGCCATGGGATTC	CAGGAATGCAATCTAAGGCC
1485009	CCTAACCCTCTTCTGGAAAA	GACCTATAAAGACAGGAATGC
7681947	ACCAGAACAGGACGAATTAG	CCCCAATGTTATGCATACTC
1816432	GTTCTAGCCTTACTGAGATAG	GCTTAAAGACCTTCAAACTAC
1485018	AAAGATCGCTTGAAACTGGG	TTGCTCTGTTGTCAAGGCTG
1485017	TGACCAGGCTGGTCTAAAAC	TAATCCCAGCAGTTTGGGAG
7438397	GAGATCTAGCAATCCTCCTG	GAAACCTAATTTTGAGCCTG
6834311	TCATTGTGTTACAGATGCTC	CCAGGCTCAAAATTAGGTTTC
1368717	AAATCTGCAGACAAGGACAG	AGCAACCTGGTTTTAAGAGC
1017391	ATGCACAAATGACAGTAGGG	GGACATTGTAGTAGTCAGGG
2870701	ATGCACAAATGACAGTAGGG	GGACATTGTAGTAGTCAGGG
7679839	CCACAGGAACTATTTGCTGG	GGAACAGCACAAGCAAAAAC
1385404	CCCAGCATCCTTGCTAATAC	CCTGTCAAGATGCATAGGAG
1368716	TGGGAGAACTGGGAAAATGG	TGCTACATACTGTAGCAGCC
4693316	ATCCTGTACTGCACTTCAGC	ACCCTCCATGGGAGTTTTTC
1905707	TTTTCTGTAGTTTCCCGAGC	AATGCAATCCACAGAGCAAG
1905708	GGAACAAATGAAGATAGATG	GGACCTATGTTTTATGAGTTC
1905709	TAGAATAGTTTCTCAGTGTC	GAACTCATAAAACATAGGTCC
3912442	CATAACTCAGGCAAAACAAC	GTTGAAAAAGCTCTATATTGG
2082553	TGGAGCCCACTCAGATATTC	CCCTGGAACATGTAAATGTG
6831638	CCATATACTTACCTTTACTG	AATGTGAGTTCTGAAGATGG
5860329	CCATGCTGGCTTTTGTGAAC	TCCTGACCCTTAGTCCAATG
2870702	CAAAGAATGAGAGGGAAGGC	TGTGGCCTACCAGAATTTGC
2870703	AATAAAGAGGACAGCAGGAG	CACAGTTGTACTCCTAGCTC
1948016	GAATGAATGCAGTGTGCCAG	TGAACCTATCTTGCAGGTGG
6835836	TTATTTAGGAAGCACTCAGC	GAGTCCAAGACTAGTAAAGAG
1994253	TTCACCTTTCTGTGTTCTCC	AAGAAGAAGAACAACTGTAC
1905710	CATTAAGTGAAGAGCTATTCC	CTTAAAAGCTATTCGTTAAGC
1485019	TTTAAGCTCCCCAAAAGGTG	TACTGTGACATTCCCTTCTC
978191	AGGTCTTATCTCTTGGAATG	CCCATTCAGATATATAGACAG
1385405	CATTCAGACCTGTGACAGGA	TGATGGGACTGTTCTTCAGG
7694361	CTAGTGATTCTGTGAATGTTG	CAATGTGATTGTGTTGGAAG
1905711	CAGTTAACACTCCATATCCAC	GAGCCTCAGTTTGTTGAATC
1905734	AATTTCAAGATCAGAGATCC	CCTAACATGAGTCCATTCTG
1485012	ATACACCTATGCTAAGAAGC	GCAGAAAAACTAGTACGATAC
1485013	ACACATCATAGTTTTACTGC	GGATGAAACTATGTGAAAGC
4692981	GCTAGCCTGGAATCAAACAC	CCGAAAACAAAAGCACCCTG
7670552	TTTGGCTGCAATCAACCATG	ATCCCAAGAGAAATGAAAAC
7670932	CCTTGAAAGCATGCTAAGGG	TGAGTCCCTATAGATTTGTC
7688091	TGGATTGTGCACTTTGGATG	ACCAGACTCTCTCTTTTTTA
7440540	TGCACAGACTGTTAAGGGAG	TCATCCTTTAAGCCTTGCCG
2171000	TGAGCCAAGTATGGAAAAAC	CTGGGATAATTGTTGGGATC
2870704	ATGCTCTTGGAGCTCAGAAG	ACATATTTCCCTGACCTCCC
7655758	GTCAAAACCTCTTACTGTGC	CATGGCAGCCTTACCAAAGA
7661436	ACTGAAATTCCTTGCTGAAC	TCTCCAGGTAAACTTCCAAG
7662289	AAAACAGTGAAAGACCTTGC	TATTGAGGCCGAGTAGTCTG
7667044	ACTGATAAAACAAAGGAGGG	GAAAGCAAGGTCTTTCACTG
7691929	TTTCTCAGCATATGTTCCAG	ATCAGTCTCAGGGAAGATGC
5860330	CTGCCTAGATGAAACAAATG	CTGCTGGAACATATGCTGAG
901013	AAGGTGCATCCCTCAGAAAG	GCCTGGCTGATCTAAGGTTT
901012	CTGAAATAGTGAGGGTAGTG	AAGATGTGGAGAGAACAAGG
901011	AAAAGGGAAGGGAAGTGTGG	ACTCTCCTCCAAGAGCTTTC
1948018	TTTATCAGCAAGCATGTGAG	GTTTTCTGGAAAACTTTTACC
2870705	TAGTGCATGAGAAAGTCTAC	TTGCTTATCTGACTGGCAAG
1948017	GTTTAACAGCAACCATTGAGG	CCCCAAAGGTATGTTAAGAG
1905733	TGGAAGAGTATTAACTTCTG	TTAGTCTATTGATTGAAAGC
1385408	ACTCTTCCAAAGACTGATGC	GGATGTGGGGTTATAAGAAC
1385409	TTGCCTCTTCCAGTCTATTG	CAGAGGAGAGACATGATCAG
1385410	AGAGACAGAAGAGACACTAG	CCCCATCCAAATACATTGCC
1485026	GAGAAGAAGGCATATGTGAG	CTCACTCTTTCTTCTGTCTG
1485027	TGGTGATGATAGGTGCTGTG	ACACAGGTGCTTTCTAATTG
2904483	CTTCTCTCCATGAAACTCAG	AGGAGATATCAAGACACCAG
1385406	CTCCATTATCTGTTTGCAGT	CTCTGAGTAGTGTTTTCTGG
1905732	CTGTTCCTTGTTTCTTCTGC	GTGGTGTTTGCCATTAAAAG
2046418	AGGAGAGAAGTCTATGTCCC	TTCTTACATTCTAGAGCCTT
2200377	GGATGGACTATTGTTTTCTT	CCTATCTTGATTAGATTACAG
1905731	TCACTGCTTAGAAACTAATC	AAACACCTTTCTGAGAATTG
1905730	AAACTGAAGCAGATTCTGAC	CTTTGTTCTTTCAATTCTCAG
975713	GCTAATTTTCCTAGGATCTC	AGTGAGCCCCAAAATCTGTG
6820985	CTAAGCCTAATAAAGGAGGG	ATATATAGAAAAGACTGGAG
7670441	CAATAATGACATGCTCTTAC	CGTATAGCAATCATACACAAG
6810794	ATGATGGAGCTCCAGAAAAG	CAGAGATATTTAGTGGCAAC
7676623	TTCAGAGCTCACTTTCCAAC	GGGGCTACCAAACTTAAAAG
1154861	GACACTGAATCCATAGATCG	CCTTTTTAAATAACCAGTGAC
1032125	CCTCATGCTTTAATAGGTAG	CACACATTTCTATAGTACTTG
1485022	AAATTAGGCTGAAGGAACAG	GCTTGATGGTTATTACAATGG
1485024	CGTTTCCACCACCTGGAAAT	CTGGCTTTCTTCCATTGTTG
3913651	CCTTGAGTATCTAAGAAAGGC	AAAATATACTTGTTTTGAGC
4693319	TCAAGAGTGGAAAGGGAGAG	CAGCTCCACTCACTATCTTG
1872383	TGGCCTCAAATGCATGTGTC	CTTTGGAGCTATCCAAATGG
2200376	GTGTTTGAGAAAGAAGTGTG	GAGTTCAGACAGAGTATGAG
7668090	GTAGGCCTGAGAATGCATTG	GACATTCCATTATTCCTCCC
7692930	ATGAGAACACATGGACACGG	TCTCCTAATGCTATCCCTCC
967096	GATTGGAAAAGGGCAACAGG	TCCATGAGAATGTTCCTCAG
6822249	AAAGGATGTTTCCATTTCTC	CCTAGAAAGGTAGTTGATGC
6532405	CTCAGGATCATTGAGACTTAG	CTTGAAAGATAACTGCGGAC
1017897	TGCCCAAACTGCAAATACAC	GCTACATTAAGTGAATTCTAC
7672674	CTACAACAGACAAGGATGGG	CAATGCCTTCAGCATTTTCC
7694568	CCATTTCTAATGGGTACCTC	TGCCATACACTACAACAGAC
2904484	ATTTTCACATTGCTTTGCCC	GAACAAGCAGAGCAAGTAAG
7340830	CAATCTCAAAACAGTGTTTAC	ACTAAGAAAGGAGAGAACAG
1485033	CTGGCTGGAATGTTAATAGG	TGTGCTCCTCAGTACATAGC
2870706	CCTGCAGGAAGAAAATAGGC	AGGGAAGCAAAACTAAGATG
1905729	GGGAATTACTCTAGCTCTTC	AGGAAAGAGTAGGCCAAATG
4693320	TCAAACTAGTAGCCCACAAA	GTATAGATTACAGTAGATGTG
6848749	CCATCTTTTTGTCTGCATTC	CAGTCAATTTTCATATTGCC
6532406	GCATGGCTCTTAGGAGAAAG	GCTGGAAGGGAAAATGGTAC
6532407	TTGTTTTCCTAGGCTCCAGC	TGGCTACAATAGGGAGACTG
1905728	GCAAGTTCATTTTCATATAGC	TAAAACAGTGTGAAATTTTG
	G
6819866	CACACATAGCTCTTCTGTAAG	GCCTCGAGGAAAAAAAAATAG
1905727	AAGGGATAACAAGACAAATG	ACCCCATGATCTACTATTTC
7674069	CAGTTACTCCAAACTTACGG	GGTAACAAAGGCACTCAAGG
1905724	GCTCTCAGTGTCTTTTAATG	CGCATATGATTAGCTACTTC
1905723	GGGTTTATAAGCCTTTTCTTC	CTTGCTAACAATGAAAAGGTG
1485020	GTTGTCATTTTATGTCCTCCG	TAGAGTCACTGCCAATAAAC
8814101	GAGGACTTCAATGACTTTGC	TCAGACACAAACATCTGAAG

TABLE 18
dbSNP	Extend	Term
rs#	Primer	Mix
1433661	CATGGGATTCCAGGTGT	ACT
1485009	CCTCTTCTGGAAAATCTTAA	ACT
7681947	ACGAATTAGTCCAAGGA	ACT
1816432	TGAGATAGTTAATTTTGTTTTCCA	ACT
1485018	CAGTGAGCCATGACTGT	ACT
1485017	GGTCTAAAACTCCTGAGAT	CGT
7438397	CTCCTGCCTTGGCCTCC	ACG
6834311	GAAGAGAAAGAGATGGC	ACG
1368717	AGGGATCGGAAATTTAAGAAGAA	ACT
1017391	AATGACAGTAGGGATTATAGTAA	ACT
2870701	GACAGTAGGGATTATAGTAAG	CGT
7679839	ATTTGCTGGCCCTTTAA	ACT
1385404	CCTAAAGCAGCCACTGT	ACT
1368716	CAATGAGAAATGCCAGC	ACG
4693316	GCGATAGAGTGAGATTCTG	ACG
1905707	TGTAGTTTCCCGAGCTAGATT	ACT
1905708	AGATAGATGAATGGAGAACCC	ACT
1905709	GTTTCTCAGTGTCATCAAATA	CGT
3912442	AAAAAGACATATCTTCTTTTAGTG	CGT
2082553	CCACTCAGATATTCCATAAC	ACT
6831638	CCTTTACTGTGATATTTATTTCTT	ACG
5860329	GCTCCTGAAACATATTCATG	ACT
2870702	GAAGGCATTATGACATGAAT	ACG
2870703	TTGTTTATTCAAATCTGCCA	ACG
1948016	GCCAGTAATCTCTCCAATGAT	CGT
6835836	CACTCAGCTGAATAGACA	ACT
1994253	CTGACTCATACATCCTTTG	ACT
1905710	GAGCTATTCCAATGTGCT	CGT
1485019	AAGCTCCCCAAAAGGTGTTTAATT	CGT
978191	TGTACAAATCTGAGGGC	ACG
1385405	CCTGTGACAGGATTCCAGCA	CGT
7694361	GGATTACAGTAGTTTCCC	ACG
1905711	CCATATCCACAGGTTCT	ACT
1905734	CTTAGCCACTCTGATAATCT	ACT
1485012	ACACTGCAAAAAGCACT	ACT
1485013	TGCAGAGATAATGTATGTAGAA	ACT
4692981	TCAAACACAGTTTATATGAGATAA	CGT
7670552	CAACCATGCTGCTATGA	ACT
7670932	CATGCTAAGGGAAAGAAG	ACG
7688091	GGATGGGTGAATTGTATATTAT	ACT
7440540	TTAAGGGAGAGCATGAAA	ACT
2171000	GGTAGAAATGGACTTTGA	ACG
2870704	GCAGCTTCCTAACAAAAA	ACT
7655758	GCCTTTGAAAGAATCCAA	ACT
7661436	CTTTTTTGTTTCTATCCAGG	ACG
7662289	TGCTTCTTTATTCCCCA	ACT
7667044	GCTTTGTTTTTGATGAGTG	ACG
7691929	TTAATTCCTGAGACGTGT	ACT
5860330	CTTGCAAGTGATTAAAAAAAAAA	ACT
901013	GAAAGCATTCATCTCACTA	ACT
901012	GTAGTGTCTACAAAGGGTATA	ACT
901011	GTTGATCTCCTTCCTGG	ACT
1948018	GAGAAAGTAGACTTTCTCAT	ACT
2870705	CTTTCTCACATGCTTGC	ACT
1948017	CAGCAACCATTGAGGGTGAAT	ACG
1905733	TGTCCTTGACTGATTTTTAG	ACG
1385408	TCTAGGAAGTATGAGATGG	ACT
1385409	CAATAGTAACTGTCAACTGT	ACT
1385410	CAAGGAAGCTAGAGCCA	ACT
1485026	CTTCAATTAGAAAGCACCT	ACT
1485027	GTGCTGTGAAGACAAATTA	CGT
2904483	GGATTGTTTCTTCCTCT	CGT
1385408	TGTATACAGAAAAAAGCATGA	CGT
1905732	GTTGGTGCAAAAGTAACT	ACT
2046418	GTCTATCTCCCTGACAC	ACG
2200377	AAAAATTAAAACATTCACTGCTTA	ACT
1905731	TTCTGTAATCTAATCAAGATAGG	ACT
1905730	TCTGACAGGACTAAACAA	ACT
975713	GGTCACCTAAGGATTTTACA	ACT
6820985	AAAGGAGGCCTCTACCC	ACT
7670441	CATGCTCTTACATGCAAATA	ACT
6810794	CCACAAAACCAAGCTTATTA	CGT
7676623	TCTTCTTTAATGTGATGGTAC	ACT
1154861	GAATCCATAGATCGTATACTAAT	ACG
1032125	ATTTATATTCCGCCCCA	CGT
1485022	TCCAAAACGACCAGTCA	CGT
1485024	TTCATGCTAACTGATTATCAAAT	ACT
3913651	GGCAAAATTCCATGGCC	ACG
4693319	GAGTAATTGGACCTCTAC	ACT
1872383	CCATTCCATATCCTTACC	CGT
2200376	TTTTACATCTCACCCCA	CGT
7668090	GAATGCATTGGAGTGAG	ACT
7692930	ATCACACTCCGGGGACT	ACT
967096	AGGGCAACAGGGACACA	ACT
6822249	GTATTCACCATTGCAAAAA	CGT
6532405	GAACAACTGAAATCTGAAGTA	ACG
1017897	AACTGCAAATACACATTTCA	ACT
7672674	GTTTTGAACACTTAATGTTTG	ACT
7694568	GATTTCACCCCTTTCCC	ACG
2904484	AAATGTTCTCCAAGAAAGAT	ACT
7340830	GTTTACATATGAGGAAATGTAG	ACG
1485033	GCCCTCTAAAGACATGA	ACT
2870706	GAAGAAAATAGGCTGATTTTAT	ACT
1905729	CTAGCTCTTCAGAATTAATTGG	ACT
4693320	CCCACAAAGTCTTATGCA	ACT
6848749	CCAACAGAGAGAGGTATTTA	CGT
6532406	CATGGTCTACACACCTTTA	ACG
6532407	CCTTGTAAGACTACCTGAA	ACT
1905728	CAGTTAATAATTGTAGATCCATG	ACT
6819866	GCTCTTCTGTAAGAAGTCT	CGT
1905727	ATGATTGTAGATCATTTTGATGTA	ACT
7674069	TTCCTAACTCTTCACCTT	ACT
1905724	CATTGTTAGCAAGTGGAA	ACT
1905723	GAGCATAAAGATGCTCTCAGT	ACT
1485020	CTCCGTTATCTCCATGT	ACT
6814101	GCAAATGTAGTTGTATGTAATTT	ACT

Genetic Analysis

Allelotyping results are shown for female cases and controls in Table 19. The allele frequency for the A2 allele is noted in the fifth and sixth columns for control pools and case pools, respectively, where “AF” is allele frequency. Some SNPs do not have an allele frequency disclosed because of failed assays.

TABLE 19
	Position in							Low BMD
	SEQ ID	Chromosome	A1/A2	Control AF	Case AF			Associated
dbSNP rs#	NO: 3	Position	Allele	(High BMD)	(Low BMD)	p-Value	OR	Allele
1433661	206	94744306	T/C	T = 0.11	T = 0.17	0.0089	1.64	T
				C = 0.89	C = 0.83
1485009	243	94744343	A/G	A = 0.00	A = 0.00
				G = 1.00	G = 1.00
7681947	2467	94746567	T/C	T =	T = 0.89
				C =	C = 0.11
1816432	3550	94747650	A/G	A = 0.56	A = 0.61	0.0779	1.23	A
				G = 0.44	G = 0.39
1485018	4994	94749094	C/G	C = 1.00	C = 0.99
				G = 0.00	G = 0.01
1485017	5167	94749267	G/T	G =	G =
				T =	T =
7438397	5193	94749293	C/T	C = 0.94	C = 0.94	0.8804	0.96	C
				T = 0.06	T = 0.06
6834311	5273	94749373	G/A	G = 0.51	G = 0.56	0.1212	1.20	G
				A = 0.49	A = 0.44
1368717	5733	94749833	A/G	A = 0.10	A = 0.13	0.1573	1.31	A
				G = 0.90	G = 0.87
1017391	7817	94751917	A/C	A = 0.64	A = 0.63	0.6402	0.94	C
				C = 0.36	C = 0.37
2870701	7818	94751918	T/A	T = 0.00	T = 0.00
				A = 1.00	A = 1.00
7679839	8612	94752712	T/G	T = 0.97	T = 0.90	0.0000	0.31	G
				G = 0.03	G = 0.10
1385404	9158	94753258	A/G	A =	A = 1.00
				G =	G = 0.00
1368716	9285	94753385	G/A	G = 0.89	G = 0.83	0.0251	0.62	A
				A = 0.11	A = 0.17
4693316	10680	94754780	G/A	G = 0.57	G = 0.53	0.2431	0.87	A
				A = 0.43	A = 0.47
1905707	11866	94755966	T/C	T = 0.07	T = 0.13	0.0008	1.99	T
				C = 0.93	C = 0.87
1905708	11958	94756058	A/G	A = 0.97	A = 0.92	0.0003	0.35	G
				G = 0.03	G = 0.08
1905709	12044	94756144	A/T	A = 0.05	A = 0.06	0.8882	1.08	A
				T = 0.95	T = 0.94
3912442	12753	94756853	G/T	G = 0.00	G = 0.00
				T = 1.00	T = 1.00
2082553	15585	94759685	A/G	A = 0.00	A = 0.11
				G = 1.00	G = 0.89
6831638	17299	94761399	C/T	C = 1.00	C = 1.00
				T = 0.00	T = 0.00
5860329	18816	94762916	—/G	— = 0.00	— = 0.00
				G = 1.00	G = 1.00
2870702	24022	94768122	C/T	C = 0.66	C = 0.65	0.8596	0.98	T
				T = 0.34	T = 0.35
2870703	24994	94769094	C/T	C = 0.63	C = 0.62	0.7613	0.96	T
				T = 0.37	T = 0.38
1948016	26637	94770737	G/T	G = 0.52	G = 0.47	0.1367	0.84	T
				T = 0.48	T = 0.53
6835836	27635	94771735	C/G	C = 0.00	C = 0.00
				G = 1.00	G = 1.00
1994253	28773	94772873	T/C	T = 0.92	T = 0.87	0.0134	0.61	C
				C = 0.08	C = 0.13
1905710	29430	94773530	A/T	A = 0.39	A = 0.34	0.2784	0.83	T
				T = 0.61	T = 0.66
1485019	29876	94773976	T/A	T = 0.14	T = 0.19	0.0338	1.42	T
				A = 0.86	A = 0.81
978191	30364	94774464	C/T	C = 0.00	C = 0.00
				T = 1.00	T = 1.00
1385405	31057	94775157	C/A	C = 0.68	C = 0.66	0.6519	0.94	A
				A = 0.32	A = 0.34
7694361	31782	94775882	C/T	C = 0.21	C = 0.24	0.1629	1.22	C
				T = 0.79	T = 0.76
1905711	33400	94777500	A/C	A = 0.00	A = 0.00
				C = 1.00	C = 1.00
1905734	35588	94779688	A/C	A = 0.92	A = 0.88	0.0229	0.61	C
				C = 0.08	C = 0.12
1485012	37663	94781763	C/G	C = 0.96	C = 0.91	0.0032	0.47	G
				G = 0.04	G = 0.09
1485013	37865	94781965	T/C	T = 0.00	T = 0.00
				C = 1.00	C = 1.00
4692981	38218	94782318	A/T	A = 0.68	A = 0.72	0.1741	1.19	A
				T = 0.32	T = 0.28
7670552	39375	94783475	T/C	T = 0.11	T = 0.22	0.0000	2.26	T
				C = 0.89	C = 0.78
7670932	39559	94783659	C/T	C = 1.00	C = 1.00
				T = 0.00	T = 0.00
7688091	39833	94783933	A/G	A = 0.00	A = 0.13
				G = 1.00	G = 0.87
7440540	40135	94784235	A/G	A = 0.00	A = 0.00
				G = 1.00	G = 1.00
2171000	41698	94785798	G/A	G = 0.00	G = 0.00
				A = 1.00	A = 1.00
2870704	42249	94786349	T/C	T = 0.53	T = 0.53	0.8418	1.02	T
				C = 0.47	C = 0.47
7655758	42571	94786671	G/C	G = 0.21	G = 0.25	0.0531	1.32	G
				C = 0.79	C = 0.75
7661436	42977	94787077	G/A	G = 0.44	G = 0.45	0.7243	1.04	G
				A = 0.56	A = 0.55
7662289	43548	94787648	T/C	T = 0.24	T = 0.29	0.1625	1.27	T
				C = 0.76	C = 0.71
7667044	43631	94787731	G/A	G = 0.18	G = 0.22	0.0669	1.31	G
				A = 0.82	A = 0.78
7691929	43705	94787805	T/C	T = 0.93	T = 0.90	0.0439	0.64	C
				C = 0.07	C = 0.10
5860330	43817	94787917	T/—	T = 0.48	T = 0.52	0.1449	1.19	T
				— = 0.52	— = 0.48
901013	44374	94788474	A/C	A = 0.00	A = 0.00
				C = 1.00	C = 1.00
901012	44464	94788564	A/C	A = 0.00	A = 0.00
				C = 1.00	C = 1.00
901011	44788	94788888	T/C	T = 1.00	T = 1.00
				C = 0.00	C = 0.00
1948018	48962	94793062	A/G	A = 0.10	A = 0.19	0.0002	2.20	A
				G = 0.90	G = 0.81
2870705	48993	94793093	T/G	T = 1.00	T = 1.00
				G = 0.00	G = 0.00
1948017	49110	94793210	C/T	C = 0.12	C = 0.17	0.0068	1.60	C
				T = 0.88	T = 0.83
1905733	49434	94793534	C/T	C = 0.02	C = 0.03	0.5881	1.41	C
				T = 0.98	T = 0.97
1385408	49523	94793623	T/G	T = 1.00	T = 1.00
				G = 0.00	G = 0.00
1385409	49742	94793842	C/G	C = 1.00	C = 1.00
				G = 0.00	G = 0.00
1385410	49907	94794007	G/C	G = 0.53	G = 0.51	0.5313	0.93	C
				C = 0.47	C = 0.49
1485026	50028	94794128	G/C	G = 1.00	G = 1.00
				C = 0.00	C = 0.00
1485027	50089	94794189	G/T	G = 0.00	G = 0.00
				T = 1.00	T = 1.00
2904483	51588	94795688	C/A	C = 0.68	C = 0.65	0.4125	0.90	A
				A = 0.32	A = 0.35
1385406	52899	94796999	C/A	C = 0.00	C = 0.00
				A = 1.00	A = 1.00
1905732	54088	94798188	A/C	A = 0.48	A = 0.47	0.8078	0.97	C
				C = 0.52	C = 0.53
2046418	56538	94800638	C/T	C = 0.64	C = 0.65	0.9229	1.01	C
				T = 0.36	T = 0.35
2200377	59071	94803171	T/C	T = 0.00	T = 0.04
				C = 1.00	C = 0.96
1905731	59110	94803210	A/C	A = 1.00	A = 1.00
				C = 0.00	C = 0.00
1905730	59178	94803278	A/G	A = 0.74	A = 0.73	0.9270	0.99	G
				G = 0.26	G = 0.27
975713	61087	94805187	T/C	T = 1.00	T = 1.00
				C = 0.00	C = 0.00
6820985	61300	94805400	C/G	C = 0.99	C = 0.99
				G = 0.01	G = 0.01
7670441	62171	94806271	T/C	T = 0.67	T = 0.66	0.7167	0.96	C
				C = 0.33	C = 0.34
6810794	62783	94806883	T/A	T = 1.00	T = 1.00
				A = 0.00	A = 0.00
7676623	62983	94807083	T/C	T = 0.00	T = 0.00
				C = 1.00	C = 1.00
1154861	63908	94808008	C/T	C = 0.73	C = 0.70	0.2406	0.84	T
				T = 0.27	T = 0.30
1032125	64088	94808188	G/T	G = 0.50	G = 0.51	0.9342	1.01	G
				T = 0.50	T = 0.49
1485022	64941	94809041	A/T	A = 1.00	A = 0.98
				T = 0.00	T = 0.02
1485024	65050	94809150	A/G	A = 0.04	A = 0.12	0.0000	3.18	A
				G = 0.96	G = 0.88
3913651	68953	94813053	C/T	C = 0.40	C = 0.41	0.6884	1.05	C
				T = 0.60	T = 0.59
4693319	70093	94814193	T/C	T = 0.81	T = 0.80	0.7260	0.95	C
				C = 0.19	C = 0.20
1872383	71308	94815408	C/A	C = 0.35	C = 0.38	0.4769	1.10	C
				A = 0.65	A = 0.62
2200376	73009	94817109	T/A	T = 0.65	T = 0.63	0.6117	0.92	A
				A = 0.35	A = 0.37
7668090	74002	94818102	A/G	A = 0.61	A = 0.59	0.4762	0.92	G
				G = 0.39	G = 0.41
7692930	74294	94818394	T/C	T = 0.00	T = 0.00
				C = 1.00	C = 1.00
967096	74879	94818979	G/C	G = 0.00	G = 0.00
				C = 1.00	C = 1.00
6822249	76936	94821036	G/T	G = 0.20	G =
				T = 0.80	T =
6532405	77195	94821295	G/A	G = 0.44	G = 0.46	0.5246	1.08	G
				A = 0.56	A = 0.54
1017897	77683	94821783	T/C	T = 0.75	T = 0.72	0.3873	0.89	C
				C = 0.25	C = 0.28
7672674	78283	94822383	T/C	T = 0.81	T = 0.82	0.6661	1.07	T
				C = 0.19	C = 0.18
7694568	78331	94822431	C/T	C = 0.82	C = 0.91	0.0004	2.33	C
				T = 0.18	T = 0.09
2904484	79362	94823462	G/C	G = 0.67	G = 0.66	0.8007	0.97	C
				C = 0.33	C = 0.34
7340830	80357	94824457	C/T	C = 1.00	C = 1.00
				T = 0.00	T = 0.00
1485033	80653	94824753	T/C	T = 0.74	T = 0.73	0.6809	0.95	C
				C = 0.26	C = 0.27
2870706	80840	94824940	A/G	A = 0.65	A = 0.65	0.9576	1.01	A
				G = 0.35	G = 0.35
1905729	83203	94827303	A/G	A = 0.66	A = 0.62	0.1186	0.82	G
				G = 0.34	G = 0.38
4693320	85405	94829505	T/C	T = 0.67	T = 0.73	0.0335	1.32	T
				C = 0.33	C = 0.27
6848749	86441	94830541	G/T	G = 0.14	G = 0.22	0.0016	1.68	G
				T = 0.86	T = 0.78
6532406	86967	94831067	G/A	G = 0.98	G = 0.95	0.0460	0.44	A
				A = 0.02	A = 0.05
6532407	87121	94831221	T/C	T = 0.95	T = 0.91	0.0301	0.57	C
				C = 0.05	C = 0.09
1905728	89617	94833717	T/C	T = 0.00	T = 0.00
				C = 1.00	C = 1.00
6819866	90969	94835069	T/A	T = 0.51	T = 0.42	0.0157	0.70	A
				A = 0.49	A = 0.58
1905727	94249	94838349	G/C	G = 0.06	G = 0.09	0.1689	1.48	G
				C = 0.94	C = 0.91
7674069	95811	94839911	T/G	T = 1.00	T = 1.00
				G = 0.00	G = 0.00
1905724	96690	94840790	T/G	T = 0.06	T = 0.07	0.3032	1.31	T
				G = 0.94	G = 0.93
1905723	96731	94840831	A/G	A = 0.71	A = 0.70	0.7768	0.96	G
				G = 0.29	G = 0.30
1485020	97267	94841367	C/G	C = 1.00	C = 1.00			C
				G = 0.00	G = 0.00
6814101	97414	94841514	T/G	T =	T = 0.00			G
				G =	G = 1.00

Allelotyping results were considered particularly significant with a calculated p-value of less than or equal to 0.05 for allelotype results. These values are indicated in bold. The allelotyping p-values were plotted in FIG. 3. The position of each SNP on the chromosome is presented on the x-axis. The y-axis gives the negative logarithm (base 10) of the p-value comparing the estimated allele in the case group to that of the control group. The minor allele frequency of the control group for each SNP designated by an X or other symbol on the graphs in FIG. 3 can be determined by consulting Table 19. For example, the left-most X on the left graph is at position 44917643. By proceeding down the Table from top to bottom and across the graphs from left to right the allele frequency associated with each symbol shown can be determined.

To aid the interpretation, multiple lines have been added to the graph. The broken horizontal lines are drawn at two common significance levels, 0.05 and 0.01. The vertical broken lines are drawn every 20 kb to assist in the interpretation of distances between SNPs. Two other lines are drawn to expose linear trends in the association of SNPs to the disease. The light gray line (or generally bottom-most curve) is a nonlinear smoother through the data points on the graph using a local polynomial regression method (W. S. Cleveland, E. Grosse and W. M. Shyu (1992) Local regression models. Chapter 8 of Statistical Models in S eds J. M. Chambers and T. J. Hastie, Wadsworth & Brook/Cole). The black line provides a local test for excess statistical significance to identify regions of association. This was created by use of a 10 kb sliding window with 1 kb step sizes. Within each window, a chi-square goodness of fit test was applied to compare the proportion of SNPs that were significant at a test wise level of 0.01, to the proportion that would be expected by chance alone (0.05 for the methods used here). Resulting p-values that were less than 10⁻⁸ were truncated at that value.

Finally, the exons and introns of the genes in the covered region are plotted below each graph at the appropriate chromosomal positions. The gene boundary is indicated by the broken horizontal line. The exon positions are shown as thick, unbroken bars. An arrow is place at the 3′ end of each gene to show the direction of transcription.

Example 8

PDE4D Proximal SNPs

It has been discovered that a polymorphic variation (rs1498608) in a gene encoding PDE4D is associated with the occurrence of low BMD (see Examples 1 and 2). One hundred sixteen additional allelic variants proximal to rs1498608 were identified and subsequently allelotyped in low BMD case and high BMD control sample sets as described in Examples 1 and 2. The polymorphic variants are set forth in Table 20. The chromosome position provided in column four of Table 20 is based on Genome “Build 34” of NCBI's GenBank.

TABLE 20
	Position in
	SEQ ID		Chromosome
dbSNP	NO: 4	Chromosome	Position	Alleles (A1/A2)	genome_letter	deduced_iupac
6886495	249	5	58309549	g/c	g	S
6450498	543	5	58309843	a/t	a	W
1472456	973	5	58310273	g/a	c	Y
4700315	1076	5	58310376	a/g	a	R
4700316	1276	5	58310576	g/c	g	S
7714708	1599	5	58310899	a/g	a	R
7710479	2755	5	58312055	c/t	c	Y
2968013	2911	5	58312211	g/c	c	S
2968014	4466	5	58313766	a/g	a	R
2968015	5754	5	58315054	t/c	c	Y
1391648	5762	5	58315062	a/g	c	Y
2055297	5967	5	58315267	a/g	c	Y
2055296	5972	5	58315272	a/g	t	Y
3989138	6390	5	58315690	—/aa	a	N
4700317	6984	5	58316284	c/t	t	Y
2036220	7234	5	58316534	g/a	c	Y
7727206	8196	5	58317496	g/t	t	K
7723432	8369	5	58317669	a/g	a	R
1546221	9565	5	58318865	c/t	g	R
4479801	11084	5	58320384	c/t	c	Y
4395595	11153	5	58320453	c/t	c	Y
4395596	11187	5	58320487	t/c	c	Y
4699932	11290	5	58320590	g/a	a	R
2936201	11386	5	58320686	a/g	t	Y
7356672	11441	5	58320741	c/t	c	Y
2936200	12373	5	58321673	a/g	c	Y
1909296	12602	5	58321902	c/a	g	K
7703131	13763	5	58323063	c/g	c	S
7445308	18697	5	58327997	a/t	t	W
3087748	18854	5	58328154	t/c	g	R
4321723	19107	5	58328407	c/t	g	R
2968016	19310	5	58328610	c/t	c	Y
5868151	20074	5	58329374	c/—	c	N
1874858	20145	5	58329445	t/c	g	R
1874857	20281	5	58329581	a/c	t	K
7712922	23117	5	58332417	c/t	t	Y
4631140	23585	5	58332885	g/a	a	R
4469166	23906	5	58333206	t/c	t	Y
1078369	24046	5	58333346	c/g	g	S
1078368	24450	5	58333750	g/a	c	Y
2968006	24619	5	58333919	a/g	t	Y
2968005	24637	5	58333937	g/t	c	M
2936190	24894	5	58334194	c/t	c	Y
2409613	25030	5	58334330	g/c	g	S
4415048	25732	5	58335032	g/c	g	S
2968004	27106	5	58336406	a/c	g	K
2968003	27395	5	58336695	g/a	t	Y
2968002	28971	5	58338271	a/g	c	Y
2936191	29755	5	58339055	t/a	t	W
1498610	30988	5	58340288	a/g	g	R
6874662	31827	5	58341127	c/a	a	M
3060393	31843	5	58341143	a/aca	a	N
7729722	32773	5	58342073	g/a	a	R
7733884	32787	5	58342087	t/c	t	Y
7714489	33099	5	58342399	t/c	c	Y
7735570	36854	5	58346154	t/c	c	Y
2936193	38026	5	58347326	g/t	g	K
2291851	38397	5	58347697	t/c	c	Y
2291852	38680	5	58347980	a/g	a	R
1498602	39626	5	58348926	t/c	t	Y
1995166	39682	5	58348982	t/c	t	Y
1498603	39710	5	58349010	g/t	t	K
1498604	39745	5	58349045	c/a	a	M
1498605	39901	5	58349201	g/a	g	R
1948651	39925	5	58349225	c/t	t	Y
4699934	40356	5	58349656	g/t	t	K
4700319	40393	5	58349693	c/t	c	Y
2279737	41230	5	58350530	a/g	a	R
7720361	41733	5	58351033	c/t	c	Y
7706419	41877	5	58351177	g/t	g	K
1006431	43555	5	58352855	t/g	g	K
1353747	44066	5	58353366	g/t	t	K
1498606	44134	5	58353434	c/t	c	Y
1353748	44181	5	58353481	t/g	g	K
1553113	45022	5	58354322	a/c	a	M
2968012	46856	5	58356156	t/g	c	M
2968011	48231	5	58357531	c/a	t	K
1498608	49652	5	58358952	a/t	t	W
2936189	50393	5	58359693	t/a	a	W
1498609	51103	5	58360403	c/t	t	Y
2968019	51733	5	58361033	t/c	c	Y
6891238	54733	5	58364033	t/c	t	Y
2968010	57173	5	58366473	a/t	t	W
2968009	58192	5	58367492	c/t	g	R
2936203	58506	5	58367806	g/c	g	S
1498601	59572	5	58368872	g/a	t	Y
1498600	59738	5	58369038	a/g	t	Y
1498599	61617	5	58370917	g/a	t	Y
2936202	63980	5	58373280	c/g	c	S
7730070	64161	5	58373461	c/g	g	S
6450501	66871	5	58376171	a/g	g	R
6450502	67063	5	58376363	a/t	a	W
6889456	67084	5	58376384	a/g	a	R
6894618	67477	5	58376777	c/t	t	Y
7706044	69282	5	58378582	t/c	t	Y
7707541	70363	5	58379663	a/t	a	W
7712076	70647	5	58379947	c/t	t	Y
6892860	71834	5	58381134	c/t	c	Y
6867053	72130	5	58381430	c/g	c	S
7737269	73495	5	58382795	c/t	t	Y
6864156	74542	5	58383842	t/c	t	Y
950447	75280	5	58384580	t/c	a	R
2936196	80740	5	58390040	a/g	g	R
7719347	82579	5	58391879	t/c	t	Y
1391649	82591	5	58391891	c/a	a	M
1391650	82976	5	58392276	t/c	c	Y
1391651	83040	5	58392340	g/a	a	R
1353749	85894	5	58395194	g/a	a	R
10682149	86020	5	58395320	g/gcct	g	N
5868153	86947	5	58396247	g/ag	g	N
1363882	88922	5	58398222	c/g	g	S
2409626	89662	5	58398962	t/c	t	Y
2968018	92367	5	58401667	g/a	c	Y
954740	93154	5	58402454	a/g	c	Y
986067	94979	5	58404279	t/c	c	Y
6869400	97598	5	58406898	t/c	c	Y
5010782	98532	5	58407832	t/a	a	W

Assay for Verifying and Allelotyping SNPs

The methods used to verify and allelotype the proximal SNPs of Table 20 are the same methods described in Examples 1 and 2 herein. The primers and probes used in these assays are provided in Table 21 and Table 22, respectively.

TABLE 21
DbSNP
rs#	Forward PCR primer	Reverse PCR primer
6886495	AGTTTGCTTCCTGAACAATC	ATGCAGTTGAATCTCAATAC
6450498	GGACAGCTTTATGTTTAATAC	ACAAATACTCCATTGATGGT
1472456	GGGAGGTAAGGAATCATGAC	TTTTGAAGGACAAGTTCCCC
4700315	GGGAACTTGTCCTTCAAAAAG	CATTTTCTGTTACTCTGAGG
4700316	ATTGTGTCCATGCTTGGCAG	CCTCCTTCTATTGTGGAGAC
7714708	GCAACATACTAACTGGAATCC	GTTCTTCTGTTTGTCATGTGG
7710479	TGCTCCAAAAATGGCAATCG	ATTTCTACATCACCAGGGAG
2968013	CACCCCTAGATTTTGAAGGT	CAATGACACATTCCCTCACC
2968014	CCTTCCCTCCTTCATTCAAC	TGGATTCAGATTACGGGAGG
2968015	TGTCAGCTGTATGGTGAAGG	AACGCCAAGCCTATTTTCAG
1391648	AATTTGGTAAGACAGAGATG	TTGCTCTGTTCCTGAAAGAC
2055297	AAGCTTTGTTTCTCTTTCTC	AACGATTCTACATCTGCCCC
2055296	AAGCTTTGTTTCTCTTTCTC	AACGATTCTACATCTGCCCC
3989138	GCCTTCCCCCTCCTTTAAAA	CATTTCAGGATGAATACTTG
4700317	TTTTCCTGATATGATGAATG	CTAGTAATGCAGCAATAGTG
2036220	TGTATCCCAGGACATCTAGC	AACATTCAGGAAATTTCAGG
7727206	AAGGACTGGGTTTGCATTTC	CTAATCTTGGCAACATTCTG
7723432	TGAATCTAGGAGTGGATTTG	AGATCTGACCATGGTGATAG
1546221	GGAAAACTCCTTATGTTGGA	GCTGTATATTATATTACTGTG
4479801	ACTTCTAGAAGAAAACACAG	TATGCTTCATGCTTTTGTGG
4395595	AAACCACAAAAGCATGAAGC	TGGCTTGTTGTTCTGTTTTC
4395596	ATATTCCCTTCTCAAGTCTG	CTGGACTTTACCAAATTTCTC
4699932	CCATCTTTTCACTGGATTGTC	CAGACTTGAGAAGGGAATATG
2936201	GCATCTCACTGTGGTTTTAC	CACAATAAGAGGACAATCCAG
7356672	GACCATCATTAGACATTAGGG	TTAATTTGCTCTGCATCCAG
2936200	TCACAACTGGGAATTCACTG	GAGCTCCACCATTATTTCCC
1909296	CCGAGTAGCTGGGATTACAT	TGGTGAAACCCTGTCTCTAC
7703131	AGAGAAGAAGGCTTCAGACG	TTTAGCTTCTTTGCGATGGG
7445308	ATACAGCGGTTGGGACTATC	GCACATTGTGCACATGTACC
3087748	TAGTCCATAGGAATCTGCTG	GCTGCTGTACATTACAACAC
4321723	GAATTTTACTTGGAACCCTGG	CCTATCTTACTACTGAACTC
2968016	GTAAACCTTGGACTTATGGG	ACGATGTTACCCTCTTTTCC
5868151	TAGAATAGACTACATCCATC	GGATAAGGAAGTTTCTTAGG
1874858	GCCTAAGAAACTTCCTTATCC	ATATTGCCAACTAGGAGTAC
1874857	ATGAAGACTTTACTGAAGGC	CATACACTACTAACCTGTTGC
7712922	CTCTTTCTAAGGGCTTCTGG	AGAGGAGTCGGACTTTGTTC
4631140	TTGAAGTTGAGAGGGTCTCC	AAGCAAAGCACAAGCAACAG
4469166	TTAGGAGGGATGAGGAATGG	CTCCAATTGCACTGGGTTAC
1078369	TCCCTGAGCCTCTGTTTTCC	ATATGTCCCCACCACACTAC
1078368	ATGACTCATGGAGGCAACAG	GGAGAGCGATTTATGGATGG
2968006	CAGGGTTCATTTGGTGAAAC	ACTGACCTGTCTGGGATTTC
2968005	ACTGACCTGTCTGGGATTTC	CGCAGGGTTCATTTGGTGAA
2936190	AACAGACCTCCAACACAACC	TAGGATAAACTCACGGAAGG
2409613	AATAGGGTCAGTGGGATGAG	CCCACCTCCAAATCCTTTAC
4415048	CACCCAGCCTCAGAATATTT	TTCCCCACCAATACAACATC
2968004	CCCAAACATTATCTTCTGGC	GATCTCCATAAGGGTAAGTG
2968003	ACAAGAGGACAAGTGTTTAG	GGCCAGAGCTGTTCATAAAT
2968002	CCTTTGACTTTCCACAGAAC	ACACTCACTGGGTTGGGCTA
2936191	TGACCTTGATAACCTGGCTG	AGCTGTGCATATTGACTTCC
1498610	CATTAAATCACCACAGCAAC	TCCTTAGGCAGAATGGTC
6874662	ACTCTAGCCTGGGCAACAGA	ATGTCTTGTAGTTTCCAGTG
3060393	CCAGTGTATAGATCTTTCACC	AGAGCGAGACTCCATCACAA
7729722	GAGGAAAGTGTGTCTATTCAG	CCATACATCTGATAAGAGGC
7733884	TGTATCTCTGTTGGCCATTC	CCATACATCTGATAAGAGGC
7714489	AGGGTCTTGCTCTTTTGTTC	ATTGAGCCCGAGAATTTGAG
7735570	AGGATCTTCATAGAAGTGGC	AACTGAGGCTGTTTTCTCTC
2936193	CTTAGCATACAATGGGCACC	ACAGTGCCTACTATTGTCAG
2291851	GTTTCTTGCTTGTTGATGGC	TAGCCTTGGCCAAGAATTCA
2291852	ATCCTGATCTCTTACTAGTG	TTCTGTGTTCTCTCTTGAGG
1498602	CAGTAGTTTTTCAGCTAAATG	ATCCAAACATAGATCTCAAC
1995166	GCCACTTAGCAATGTGCAAG	GGGAAAGTAACTAGCTGCTC
1498603	GTGTGGTTGATTCTGTGTAAG	ACTACTGTCGAATCACCTTG
1498604	GCTTGCACATTGCTAAGTGG	CCATGGGCCTGAGTTCTTAA
1498605	CTGTGGCATTAGGCACCTTT	AGGACACTGGACATATTCAG
1948651	CAGATGAGAATAAAGGTGCC	TATTGCTCTCTTACTGGGAC
4699934	AGGACTGGTAATGTTGTGAG	GACCTTGTAAATAGGTGGCC
4700319	GACCTTGTAAATAGGTGGCC	GCTGAAGGATTCAGCCAGTA
2279737	AAAACCTCTCAATTTATTTC	ATGGCCTCCAACAAGGTAAG
7720361	ACTATGCGACTTTTCAACTG	ACCCCCTCGCCATCCGCTA
7706419	ACCCGGCCGCGGCTGATTCAT	GCCCCGCCTGCCGAGCCTT
1006431	CCTTGCCAGGTGAATTAAAG	CTATACAGAGCAGGTATTTT
1353747	TGAGAAAGTTGGAGTGCAGG	AATCATTGGTTACAATGAAG
1498606	ACTCCAACTTTCTCAAAGCC	TTGGTGAAGATGGAGGAAGG
1353748	CTCCATCTTCACCAAGTTCC	GAAATATAATGTGTGGAGCC
1553113	AGCCTTTAGGGAGTTTAGCC	GCACCAAATCTTGCTAAGTC
2968012	CAGTTCTATCTACTGTAGAC	CAGTTCCAGCTTCTCTTCTC
2968011	ATAATGGTTGCACTGACTTC	ACTGTGTGACATGGGATCTG
1498608	GAATCCCTGTTCATTCCTTG	ATAACCTCGGGGTCCAGAAA
2936189	TGATCTCAGACTTCCAGCTC	TTTGTCTTAGCTCAAGCTGC
1498609	TAGCTCAACTGTTCTTCAGG	CAGAGTGAGTGTAAATATAC
2968019	CCCAGGCCAGTATTACTGTT	TGACATTTACAGGCACTCAG
6891238	CCTTTATTCAGGCTGCAGAC	TGTGGTTTTAATGGCTGTGG
2968010	ATGTATGATTCACTCTGATG	CAAACCAATTGGTAGATTTC
2968009	GTAGGCAAAGATGAATCACG	TGTTTTACCTAGCAAGGAGC
2936203	AGTTCAGAGGTTCCAGACAG	AGCCAAGCTTGCAAACTCTG
1498601	TTGGTTAGATCCAGCTCTGC	TAAGAGGAACAGGGATCTGC
1498600	ATATAGGTACTGCTTTCTCC	CCTTGTTTCCAAATCTGAGC
1498599	GGAACATTTGGCTACATCATG	CCACAGAGCTGATTTAATTTC
2936202	TGTAAGAGGAGGGTGTGATG	TGACTCTGCAGGACTGTCTG
7730070	AAATATACTTGGGTAGAGAG	GATAGCTAACACATTTCTGAC
6450501	AGCTAGACTACATAGCCTCC	TGTAGGACATGACAGCAAAC
6450502	TAACAAGTCAAAACAGATGG	ATTTTCAATATTCTGCACAC
6889456	GGATCTCTATTAAACCTCTC	TTCAAGGGTTACTGATACTC
6894618	CCATCAACAGATGAATGGAC	TCCTTCCTTTTTTAGGGCTG
7706044	GACTTGGTATTTTGTGAGGG	TCCACCATGTGAGGACAAAG
7707541	TTTTGAGTCATGAAAAACTG	CACATGTATTAATTAAGTAGG
7712076	AAGCCATACCACAAGCATTC	GTTCACGTGAAATACACTACC
6892860	CAGAAGATGCAATGAAAAGAC	AGAACAAAATGTTAGGACGC
6867053	TTATCCGCATTCCACTCTTG	ACACTGGTCCTCATAACAAG
7737269	TTTAAAGGCTCGCACCTCAG	TCCGAAGGAAAGTGATTCTC
6864156	GTTGGATCATCAAAAAGTGG	GGCTAGAGTACTAAAAATCAG
950447	GTGAGTAGTCTGAATTTGTC	AGGCTTCAGAATCGGTCATC
2936196	CTCCAAAACATAGTAAGTGC	TGAACATTTAGAATTAGGGG
7719347	ACATTCTGAGGTCATGCAGG	CTCAGAGCCTGCATTATCTC
1391649	GCTAATGCTTTACTCTGGTC	CACACTCCAAAATGTGCAAC
1391650	TTCAGCTGATACTTGCTCCC	CTCTACCTACCAACATCGTC
1391651	TTGACGTGATAGAAGTTTGG	CTGAAAAGGGTGGCTTTCTC
1353749	TATAGAGCAAAAAGCCAAGG	CCACTTCTACCACATTCTTC
10682149	GGGAGAATATAACCATTAAGT	GAATTGCATTTTATCCAATC
5868153	CATAGGGACTATTTAAACTTC	GAGTGTTCTTTAGAGATTAAG
1363882	CTCACACAGGCAATGAGTAG	AGCCACTACTTCTCAATCTC
2409626	CCTGGTCTCAAGCAATCATC	ATAAGGCCAGATGTGGTGAC
2968018	GCAGAGAGATGAGAGGAAAC	CCTCATATCTAATCTCTCCC
954740	ATACTTGGGAGCACTCAACG	CAACAAGACGGAATCCAAAG
986067	CTACAAATTGCTTAAGCAGGG	TGATAGAGTAGAGAGACTCC
6869400	ACAAGATCGTTGAATGGTGG	GACTTTGTATACTGCCACTC
5010782	AATCTGTGGGAGTTAGTGGT	TTCCTCATCCTCATCTTCCC

TABLE 22
dbSNP	Extend	Term
rs#	Primer	Mix
6886495	CCTGAACAATCTTAAATGC	ACT
6450498	GTCTTATGCATTTTGAAGG	CGT
1472456	GGAATCATGACTACTTGGA	ACG
4700315	GAAACAAAGCAAACGAC	ACT
4700316	TGCTTGGCAGGCTTTTT	ACT
7714708	CAGTATAAGTAATTTGGCCC	ACT
7710479	GAGCATTTTAATTGCTTCC	ACG
2968013	AGCTGAGAGCAGCCATG	ACT
2968014	GAGCTGTTTCTTTCAGTTT	ACT
2968015	ATGGTGAAGGTGGAGGA	ACT
1391648	TCAGCTGTATGGTGAAG	ACT
2055297	GTGCCTGACATAGAGTAG	ACT
2055296	GGCAGTGCCTGACATAG	ACT
3989138	CCCCTCCTTTAAAAAAAAAAA	CGT
4700317	CAAATGTAACAAAGTGCCA	ACG
2036220	GCCTCCCTGGCCTCTGC	ACG
7727206	AATCCTGTCACCTATGG	CGT
7723432	GTGCCTTGAATTAAAGAATC	ACT
1546221	CTTATGTTGGAGAGGTC	ACG
4479801	TAGAAAGTAGTTGTGATCTTG	ACG
4395595	AAAAATGGTATACTGGACTTTA	ACG
4395596	CTGTGGCTTGTTGTTCT	ACT
4699932	CACTGGATTGTCCTCTTA	ACG
2936201	CCTAATGTCTAATGATGGTC	ACT
7356672	CACAGTGAGATGCCACA	ACG
2936200	CTTGGATAACTGTATGCCT	ACT
1909296	TTACATGAGTGCACCAC	CGT
7703131	GGTAATAAACTTTTCCGAG	ACT
7445308	GTTGGGACTATCTACTTTTTT	CGT
3087748	CATAGGAATCTGCTGCCTCAC	ACT
4321723	ACTTGGAACCCTGGTATTTT	ACG
2968016	GGGGCAAAAAGACAATAAA	ACG
5868151	GAATAGACTACATCCATCAAAATA	ACG
1874858	GAAACTTCCTTATCCAAGGCC	ACT
1874857	GCAATTAAAAGTGGTACAATAA	ACT
7712922	CAATAAAAGGAAAGAGACTTC	ACG
4631140	AGAGGGTCTCCTGTAGG	ACG
4469166	GAGGAATGGAACTATTTTTAAAC	ACT
1078369	CTCTGTTTTCCCTGTAAAT	ACT
1078368	GCAACAGGTGAAGCTGA	ACG
2968006	GACTGAACCTTTAGGAGA	ACT
2968005	TTCTCCTAAAGGTTCAGT	CGT
2936190	CCAACACAACCTCTTAA	ACG
2409613	CCAAGTGACTAAAGCAGAT	ACT
4415048	AAAACCTAATGCTCAGTTAAA	ACT
2968004	TCTGGCATAAAGTAAACTAATC	ACT
2968003	GGGGACTAGTCAACAAA	ACG
2968002	TCTGATGAGGTCTTCTAC	ACT
2936191	GGCTGAAGTAGTGATAGG	CGT
1498610	GAAGCACAGAAGGCAAC	ACT
6874662	CAGAGCGAGACTCCATC	CGT
3060393	GATTTGCTCCTAAGTTTTTT	ACT
7729722	GTGTCTATTCAGATCCTTTG	ACG
7733884	TTGAGGAAAGTGTGTCT	ACT
7714489	GGCTGGAGTGCAGTGGC	ACT
7735570	TCCACATTATGCAACTACA	ACT
2936193	AAAAGTCAGTGTAAGAGTTC	CGT
2291851	TGATGGCATTGAAGCAG	ACT
2291852	GTTTTTATTGTGCTTTTCAATCT	ACT
1498602	GAATTTGAGCAGCTAGTT	ACT
1995166	TGTGCAAGCATTAGTGTAT	ACT
1498603	GAGCAGAATCAAAAGCC	CGT
1498604	GCTCTTCATTTTCTTACACAG	CGT
1498605	AGGCACCTTTATTCTCAT	ACG
1948651	GAATAAAGGTGCCTAATGC	ACG
4699934	GTAATAGTTGCCTCGTC	CGT
4700319	TTACTAATTGTCTCACAACATTA	ACG
2279737	TCGCCGGCATGGGAATC	ACT
7720361	AGTCCCAGTCCAGGAGC	ACG
7706419	TTCATTCACTTCAAGTGC	CGT
1008431	GTGAATTAAAGTTTCAGATTGAA	ACT
1353747	GGAGAGGAGCCACAGAA	CGT
1498606	CAAGTGATTTCCCAGTAG	ACG
1353748	ACCAAGTTCCCAAAGCT	ACT
1553113	GGAGTTTAGCCAATAGTTTTTGC	ACT
2968012	GACAGTATCAATTCCTTCC	ACT
2968011	TGACTTCAACTTTACTTTCTT	CGT
1498608	CCCTAAAAACTGTTCCAGGTA	CGT
2936189	CCAGAACTGTGAGAAATAAA	CGT
1498609	GTTTTCTATTTGAAATTGAGGTAT	ACG
2968019	CCAGTATTACTGTTTGAATCTC	ACT
6891238	CTGCAGACATTTCCTAC	ACT
2968010	TTCACTCTGATGTTTTCTATTT	CGT
2968009	CATAACCTCTGGTTTCC	ACG
2936203	GGAGGATATCCATGCCCC	ACT
1498601	AGCTCTGCCTCCCCCATTT	ACG
1498600	AGGTGCCAAGCTAGTCA	ACT
1498599	GGCTACATCATGTTTTGG	ACG
2936202	CCCTGGTAACTGTAGTG	ACT
7730070	TAGGCATGCTCAAACTC	ACT
6450501	GCCTCCTAAAAAAGCAC	ACT
6450502	GTTTAATGACATTTTAGAGAGG	CGT
6889456	AACCTCTCTAAAATGTCATTAA	ACT
6894618	GGACAAAGGAAATGTGATATATA	ACG
7706044	GGCTCATTTCCTGGGTC	ACT
7707541	GAAAAACTGACAACATATGAG	CGT
7712076	CCCAGAGGATATAGATTTCAT	ACG
6892860	GGATATTTCAGTGGTGCT	ACG
6867053	CACCATTAACCTGGTCTA	ACT
7737269	ACTCTTGATCAATAGTTTGG	ACG
6864156	GTAGTTGACAAATCTTACCT	ACT
950447	TTGTTAGTGTATTAGCCATTG	ACT
2936196	GTGCAAAGATCAATCACA	ACT
7719347	GGTCATGCAGGAAATAG	ACT
1391649	TCTGGTCTATTTCCTGC	CGT
1391650	CCCACAGGTTTTTCACA	ACT
1391651	GGGCCCCCGTTTTGCCCTG	ACG
1353749	CAAAAAGCCAAGGATATAAAATA	ACG
10682149	GTATGATCTGATTTCCATAAATAG	ACT
5868153	GAAATGAATAAATGGCAAAAAA	ACT
1363882	GCAATGAGTAGACTAAAAAAA	ACT
2409626	AGCAATCATCCCACCTC	ACT
2968018	CAGACACCCACTGACCA	ACG
954740	ACGAAGGTCCCGTCTGA	ACT
986067	CAGGGATTTAAAATGAAACC	ACT
6869400	AATGGTGGGTCATTAGTT	ACT
5010782	GCACCTACAGAGGAAAT	CGT

Genetic Analysis

Allelotyping results are shown for female cases and controls in Table 23. The allele frequency for the A2 allele is noted in the fifth and sixth columns for control pools and case pools, respectively, where “AF” is allele frequency. Some SNPs do not have an allele frequency disclosed because of failed assays.

TABLE 23
	Position in							Low BMD
dbSNP	SEQ ID	Chromosome	A1/A2	Control AF	Case AF			Associated
rs#	NO: 4	Position	Allele	(High BMD)	(Low BMD)	p-Value	OR	Allele
6886495	249	58309549	G/C	G =	G = 0.87
				C =	C = 0.13
6450498	543	58309843	A/T	A = 0.81	A = 0.85	0.1780	1.29	A
				T = 0.19	T = 0.15
1472456	973	58310273	G/A	G = 1.00	G = 1.00
				A = 0.00	A = 0.00
4700315	1076	58310376	A/G	A =	A = 0.95
				G =	G = 0.05
4700316	1276	58310576	G/C	G = 0.87	G = 0.84	0.1520	0.75	C
				C = 0.13	C = 0.16
7714708	1599	58310899	A/G	A = 0.35	A = 0.46	0.0013	1.60	A
				G = 0.65	G = 0.54
7710479	2755	58312055	C/T	C = 1.00	C = 1.00
				T = 0.00	T = 0.00
2968013	2911	58312211	G/C	G = 0.84	G = 0.80	0.1256	0.79	C
				C = 0.16	C = 0.20
2968014	4466	58313766	A/G	A = 0.22	A = 0.25	0.2834	1.16	A
				G = 0.78	G = 0.75
2968015	5754	58315054	T/C	T = 0.88	T = 0.86	0.2038	0.80	C
				C = 0.12	C = 0.14
1391648	5762	58315062	A/G	A = 0.25	A = 0.24	0.8566	0.97	G
				G = 0.75	G = 0.76
2055297	5967	58315267	A/G	A = 0.00	A = 0.00
				G = 1.00	G = 1.00
2055296	5972	58315272	A/G	A = 0.94	A = 0.94	0.8857	1.04	A
				G = 0.06	G = 0.06
3989138	6390	58315690	—/AA	— = 0.53	— = 0.50	0.3703	0.90	A
				AA = 0.47	AA = 0.50
4700317	6984	58316284	C/T	C = 0.00	C = 0.00
				T = 1.00	T = 1.00
2036220	7234	58316534	G/A	G = 0.44	G = 0.44	0.9272	1.01	G
				A = 0.56	A = 0.56
7727206	8196	58317496	G/T	G = 0.27	G = 0.25	0.3954	0.89	T
				T = 0.73	T = 0.75
7723432	8369	58317669	A/G	A = 0.82	A = 0.82	0.7671	1.05	A
				G = 0.18	G = 0.18
1546221	9565	58318865	C/T	C = 0.83	C = 0.84	0.7282	1.06	C
				T = 0.17	T = 0.16
4479801	11084	58320384	C/T	C = 0.86	C = 0.87	0.7440	1.07	C
				T = 0.14	T = 0.13
4395595	11153	58320453	C/T	C = 0.93	C = 0.93	0.7241	1.09	C
				T = 0.07	T = 0.07
4395596	11187	58320487	T/C	T = 0.01	T = 0.02
				C = 0.99	C = 0.98
4699932	11290	58320590	G/A	G = 0.13	G = 0.10	0.1440	0.76	A
				A = 0.87	A = 0.90
2936201	11386	58320686	A/G	A = 0.17	A = 0.19	0.3609	1.15	A
				G = 0.83	G = 0.81
7356672	11441	58320741	C/T	C = 0.88	C = 0.89	0.4757	1.17	C
				T = 0.12	T = 0.11
2936200	12373	58321673	A/G	A = 1.00	A = 1.00
				G = 0.00	G = 0.00
1909296	12602	58321902	C/A	C = 0.98	C = 0.99
				A = 0.02	A = 0.01
7703131	13763	58323063	C/G	C =	C =
				G =	G =
7445308	18697	58327997	A/T	A = 0.00	A = 0.00
				T = 1.00	T = 1.00
3087748	18854	58328154	T/C	T =	T = 0.21
				C =	C = 0.79
4321723	19107	58328407	C/T	C = 0.19	C = 0.24	0.1033	1.30	C
				T = 0.81	T = 0.76
2968016	19310	58328610	C/T	C = 0.45	C = 0.44	0.7959	0.96	T
				T = 0.55	T = 0.56
5868151	20074	58329374	C/—	C = 0.44	C = 0.43	0.6659	0.95	—
				— = 0.56	— = 0.57
1874858	20145	58329445	T/C	T = 0.12	T = 0.17	0.1558	1.43	T
				C = 0.88	C = 0.83
1874857	20281	58329581	A/C	A = 0.96	A = 0.96	0.5485	0.83	A
				C = 0.04	C = 0.04
7712922	23117	58332417	C/T	C = 0.05	C = 0.00
				T = 0.95	T = 1.00
4631140	23585	58332885	G/A	G = 0.10	G = 0.09	0.4903	0.87	A
				A = 0.90	A = 0.91
4469166	23906	58333206	T/C	T = 0.97	T = 0.98	0.6510	1.30	T
				C = 0.03	C = 0.02
1078369	24046	58333346	C/G	C = 0.88	C = 0.88	0.8279	0.96	C
				G = 0.12	G = 0.12
1078368	24450	58333750	G/A	G = 0.50	G = 0.47	0.3033	0.89	A
				A = 0.50	A = 0.53
2968006	24619	58333919	A/G	A = 0.34	A = 0.35	0.5216	1.08	A
				G = 0.66	G = 0.65
2968005	24637	58333937	G/T	G = 0.37	G = 0.36	0.7332	0.96	T
				T = 0.63	T = 0.64
2936190	24894	58334194	C/T	C = 0.22	C = 0.22	0.9071	1.02	C
				T = 0.78	T = 0.78
2409613	25030	58334330	G/C	G = 1.00	G = 1.00
				C = 0.00	C = 0.00
4415048	25732	58335032	G/C	G = 0.94	G = 0.96	0.2666	1.36	G
				C = 0.06	C = 0.04
2968004	27106	58336406	A/C	A = 0.92	A = 0.88	0.1210	0.70	C
				C = 0.08	C = 0.12
2968003	27395	58336695	G/A	G = 0.61	G = 0.66	0.2364	1.23	G
				A = 0.39	A = 0.34
2968002	28971	58338271	A/G	A = 0.81	A = 0.81	0.8196	1.04	A
				G = 0.19	G = 0.19
2936191	29755	58339055	T/A	T = 1.00	T = 1.00
				A = 0.00	A = 0.00
1498610	30988	58340288	A/G	A = 0.00	A = 0.00
				G = 1.00	G = 1.00
6874662	31827	58341127	C/A	C = 0.00	C = 0.00
				A = 1.00	A = 1.00
3060393	31843	58341143	—/CA	— = 1.00	— = 1.00
				CA = 0.00	CA = 0.00
7729722	32773	58342073	G/A	G = 0.01	G = 0.01
				A = 0.99	A = 0.99
7733884	32787	58342087	T/C	T = 0.96	T = 0.97	0.5282	1.26	T
				C = 0.04	C = 0.03
7714489	33099	58342399	T/C	T = 0.15	T = 0.14	0.7668	0.95	C
				C = 0.85	C = 0.86
7735570	36854	58346154	T/C	T = 0.08	T = 0.09	0.4593	1.18	T
				C = 0.92	C = 0.91
2936193	38026	58347326	G/T	G = 0.25	G = 0.28	0.3638	1.14	G
				T = 0.75	T = 0.72
2291851	38397	58347697	T/C	T = 0.05	T = 0.06	0.2114	1.40	T
				C = 0.95	C = 0.94
2291852	38680	58347980	A/G	A = 0.98	A = 0.98
				G = 0.02	G = 0.02
1498602	39626	58348926	T/C	T = 0.67	T = 0.61	0.0496	0.77	C
				C = 0.33	C = 0.39
1995166	39682	58348982	T/C	T = 0.54	T = 0.55	0.7840	1.03	T
				C = 0.46	C = 0.45
1498603	39710	58349010	G/T	G = 0.03	G = 0.02
				T = 0.97	T = 0.98
1498604	39745	58349045	C/A	C = 0.16	C = 0.13	0.2329	0.79	A
				A = 0.84	A = 0.87
1498605	39901	58349201	G/A	G = 0.88	G = 0.89	0.3713	1.18	G
				A = 0.12	A = 0.11
1948651	39925	58349225	C/T	C = 0.58	C = 0.56	0.5199	0.93	T
				T = 0.42	T = 0.44
4699934	40356	58349656	G/T	G = 0.13	G = 0.05	0.0043	0.35	T
				T = 0.87	T = 0.95
4700319	40393	58349693	C/T	C =	C = 0.98
				T =	T = 0.02
2279737	41230	58350530	A/G	A = 0.82	A = 0.80	0.3520	0.87	G
				G = 0.18	G = 0.20
7720361	41733	58351033	C/T	C = 1.00	C = 1.00
				T = 0.00	T = 0.00
7706419	41877	58351177	G/T	G = 0.89	G = 0.92	0.1509	1.38	G
				T = 0.11	T = 0.08
1006431	43555	58352855	T/G	T = 0.22	T = 0.30	0.0077	1.49	T
				G = 0.78	G = 0.70
1353747	44066	58353366	G/T	G = 0.23	G = 0.15	0.0025	0.61	T
				T = 0.77	T = 0.85
1498606	44134	58353434	C/T	C = 0.85	C = 0.87	0.3475	1.17	C
				T = 0.15	T = 0.13
1353748	44181	58353481	T/G	T = 0.65	T = 0.66	0.8645	1.02	T
				G = 0.35	G = 0.34
1553113	45022	58354322	A/C	A = 0.95	A = 0.97	0.1730	1.54	A
				C = 0.05	C = 0.03
2968012	46856	58356156	T/G	T = 0.66	T = 0.66	0.9069	1.01	T
				G = 0.34	G = 0.34
2968011	48231	58357531	C/A	C = 0.67	C =
				A = 0.33	A =
1498608	49652	58358952	A/T	A = 0.19	A = 0.13	0.0118	0.64	T
				T = 0.81	T = 0.87
2936189	50393	58359693	T/A	T = 0.63	T = 0.65	0.6021	1.08	T
				A = 0.37	A = 0.35
1498609	51103	58360403	C/T	C = 0.34	C = 0.40	0.0314	1.30	C
				T = 0.66	T = 0.60
2968019	51733	58361033	T/C	T = 0.62	T = 0.64	0.5417	1.08	T
				C = 0.38	C = 0.36
6891238	54733	58364033	T/C	T = 1.00	T = 1.00
				C = 0.00	C = 0.00
2968010	57173	58366473	A/T	A = 0.90	A = 0.95	0.0012	2.28	A
				T = 0.10	T = 0.05
2968009	58192	58367492	C/T	C = 0.65	C = 0.70	0.0959	1.24	C
				T = 0.35	T = 0.30
2936203	58506	58367806	G/C	G = 0.17	G = 0.14	0.1730	0.80	C
				C = 0.83	C = 0.86
1498601	59572	58368872	G/A	G = 0.81	G = 0.78	0.3899	0.83	A
				A = 0.19	A = 0.22
1498600	59738	58369038	A/G	A = 0.48	A = 0.46	0.5991	0.94	G
				G = 0.52	G = 0.54
1498599	61617	58370917	G/A	G = 0.69	G = 0.69	0.9739	1.00	G
				A = 0.31	A = 0.31
2936202	63980	58373280	C/G	C = 0.20	C = 0.14	0.0286	0.68	G
				G = 0.80	G = 0.86
7730070	64161	58373461	C/G	C = 0.74	C = 0.73	0.5744	0.93	G
				G = 0.26	G = 0.27
6450501	66871	58376171	A/G	A = 0.00	A = 0.09
				G = 1.00	G = 0.91
6450502	67063	58376363	A/T	A = 1.00	A = 1.00
				T = 0.00	T = 0.00
6889456	67084	58376384	A/G	A =	A = 1.00
				G =	G = 0.00
6894618	67477	58376777	C/T	C = 0.69	C = 0.73	0.1542	1.21	C
				T = 0.31	T = 0.27
7706044	69282	58378582	T/C	T =	T = 1.00
				C =	C = 0.00
7707541	70363	58379663	A/T	A = 0.63	A = 0.61	0.5874	0.93	T
				T = 0.37	T = 0.39
7712076	70647	58379947	C/T	C = 0.00	C = 0.00
				T = 1.00	T = 1.00
6892860	71834	58381134	C/T	C = 0.81	C = 0.83	0.4091	1.14	C
				T = 0.19	T = 0.17
6867053	72130	58381430	C/G	C = 0.46	C = 0.44	0.4563	0.91	G
				G = 0.54	G = 0.56
7737269	73495	58382795	C/T	C = 0.00	C = 0.00
				T = 1.00	T = 1.00
6864156	74542	58383842	T/C	T = 0.99	T = 0.99
				C = 0.01	C = 0.01
950447	75280	58384580	T/C	T =	T = 1.00
				C =	C = 0.00
2936196	80740	58390040	A/G	A = 0.00	A = 0.00
				G = 1.00	G = 1.00
7719347	82579	58391879	T/C	T = 1.00	T = 1.00
				C = 0.00	C = 0.00
1391649	82591	58391891	C/A	C = 0.42	C = 0.35	0.0162	0.74	A
				A = 0.58	A = 0.65
1391650	82976	58392276	T/C	T = 0.75	T = 0.75	0.9568	0.99	T
				C = 0.25	C = 0.25
1391651	83040	58392340	G/A	G = 0.58	G = 0.59	0.8831	1.02	G
				A = 0.42	A = 0.41
1353749	85894	58395194	G/A	G = 0.43	G = 0.39	0.1596	0.85	A
				A = 0.57	A = 0.61
10682149	86020	58395320	—/CCT	— = 0.37	— = 0.39	0.5382	1.08	—
				CCT = 0.63	CCT = 0.61
5868153	86947	58396247	A/—	A = 0.72	A = 0.71	0.7470	0.96	—
				— = 0.28	— = 0.29
1363882	88922	58398222	C/G	C = 0.45	C = 0.43	0.5228	0.93	G
				G = 0.55	G = 0.57
2409626	89662	58398962	T/C	T = 0.84	T = 0.85	0.8581	1.03	T
				C = 0.16	C = 0.15
2968018	92367	58401667	G/A	G = 0.75	G = 0.75	0.8411	0.97	G
				A = 0.25	A = 0.25
954740	93154	58402454	A/G	A = 0.00	A = 0.00
				G = 1.00	G = 1.00
986067	94979	58404279	T/C	T = 0.04	T = 0.06	0.2475	1.43	T
				C = 0.96	C = 0.94
6869400	97598	58406898	T/C	T = 0.00	T = 0.00
				C = 1.00	C = 1.00
5010782	98532	58407832	T/A	T = 0.68	T = 0.69	0.8644	1.02	T
				A = 0.32	A = 0.31

Allelotyping results were considered particularly significant with a calculated p-value of less than or equal to 0.05 for allelotype results. These values are indicated in bold. The allelotyping p-values were plotted in FIG. 4. The position of each SNP on the chromosome is presented on the x-axis. The y-axis gives the negative logarithm (base 10) of the p-value comparing the estimated allele in the case group to that of the control group. The minor allele frequency of the control group for each SNP designated by an X or other symbol on the graphs in FIG. 4 can be determined by consulting Table 23. For example, the left-most X on the left graph is at position 58309549. By proceeding down the Table from top to bottom and across the graphs from left to right the allele frequency associated with each symbol shown can be determined.

To aid the interpretation, multiple lines have been added to the graph. The broken horizontal lines are drawn at two common significance levels, 0.05 and 0.01. The vertical broken lines are drawn every 20 kb to assist in the interpretation of distances between SNPs. Two other lines are drawn to expose linear trends in the association of SNPs to the disease. The light gray line (or generally bottom-most curve) is a nonlinear smoother through the data points on the graph using a local polynomial regression method (W. S. Cleveland, E. Grosse and W. M. Shyu (1992) Local regression models. Chapter 8 of Statistical Models in S eds J. M. Chambers and T. J. Hastie, Wadsworth & Brooks/Cole.). The black line provides a local test for excess statistical significance to identify regions of association. This was created by use of a 10 kb sliding window with 1 kb step sizes. Within each window, a chi-square goodness of fit test was applied to compare the proportion of SNPs that were significant at a test wise level of 0.01, to the proportion that would be expected by chance alone (0.05 for the methods used here). Resulting p-values that were less than 10⁻⁸ were truncated at that value.

Finally, the exons and introns of the genes in the covered region are plotted below each graph at the appropriate chromosomal positions. The gene boundary is indicated by the broken horizontal line. The exon positions are shown as thick, unbroken bars. An arrow is place at the 3′ end of each gene to show the direction of transcription.

Example 9

GPX3 Proximal SNPs

It has been discovered that a polymorphic variation (rs869975) in a gene encoding GPX3 is associated with the occurrence of low BMD (see Examples 1 and 2). Two hundred thirty-three additional allelic variants proximal to rs869975 were identified and subsequently allelotyped in low BMD case and high BMD control sample sets as described in Examples 1 and 2. The polymorphic variants are set forth in Table 24. The chromosome position provided in column four of Table 24 is based on Genome “Build 34” of NCBI's GenBank.

TABLE 24
	Position in
	SEQ ID		Chromosome
dbSNP	NO: 5	Chromosome	Position	Alleles (A1/A2)	genome_letter	deduced_iupac
1478398	231	5	150385031	a/g	t	Y
1478397	330	5	150385130	t/c	a	R
1160114	582	5	150385382	g/c	g	S
1160113	589	5	150385389	a/g	c	Y
1382323	1060	5	150385860	a/g	c	Y
1160112	1066	5	150385866	g/a	t	Y
7709870	1311	5	150386111	g/a	a	R
7710643	1556	5	150386356	g/t	g	K
7730467	1655	5	150386455	t/c	t	Y
6579829	1692	5	150386492	a/c	a	M
6579830	1802	5	150386602	g/a	g	R
6579831	2061	5	150386861	t/a	a	W
6896232	2112	5	150386912	t/a	t	W
1351131	2153	5	150386953	t/c	g	R
1038074	2667	5	150387467	c/t	g	R
1478396	3115	5	150387915	t/c	a	R
6880512	3186	5	150387986	g/a	a	R
4958858	5621	5	150390421	t/c	t	Y
4958431	5735	5	150390535	t/g	g	K
4958432	5829	5	150390629	g/c	g	S
6898463	6658	5	150391458	a/c	c	M
4958859	7901	5	150392701	g/c	c	S
4130064	11447	5	150396247	g/a	g	R
4130065	11466	5	150396266	a/g	a	R
4133119	11984	5	150396784	t/c	g	R
4958860	15803	5	150400603	t/g	t	K
4958861	16257	5	150401057	t/g	t	K
4437356	17604	5	150402404	c/a	c	M
4958868	19762	5	150404562	c/t	t	Y
1478400	22367	5	150407167	a/g	g	R
6889375	22709	5	150407509	a/g	g	R
1600159	23631	5	150408431	g/c	c	S
6875892	23686	5	150408486	t/a	t	W
4608909	25599	5	150410399	t/c	t	Y
2345000	26973	5	150411773	a/c	c	M
4516840	28457	5	150413257	g/t	t	K
2054440	28669	5	150413469	a/g	a	R
707141	29908	5	150414708	c/t	a	R
707142	30105	5	150414905	a/g	t	Y
841236	30711	5	150415511	a/g	a	R
707143	30851	5	150415651	g/a	t	Y
707144	31203	5	150416003	t/c	g	R
6869405	31446	5	150416246	a/c	c	M
707145	31638	5	150416438	c/t	g	R
707146	33064	5	150417864	c/t	g	R
707148	33958	5	150418758	c/a	t	K
707150	35182	5	150419982	a/t	t	W
5872184	38332	5	150423132	a/ac	a	N
3763015	40875	5	150425675	g/a	c	Y
2042235	41624	5	150426424	t/c	t	Y
3763013	41671	5	150426471	a/g	c	Y
2042236	41825	5	150426625	g/a	g	R
1946234	42920	5	150427720	c/a	a	M
1946235	42935	5	150427735	t/c	t	Y
1946236	43001	5	150427801	t/a	a	W
8177402	43012	5	150427812	t/c	c	Y
8177403	43203	5	150428003	c/t	c	Y
8177404	43294	5	150428094	t/c	t	Y
8177405	43295	5	150428095	t/c	c	Y
8177406	43344	5	150428144	c/t	t	Y
8177407	43509	5	150428309	t/c	c	Y
8177408	43549	5	150428349	g/c	c	S
8177409	43560	5	150428360	t/a	a	W
6888961	43578	5	150428378	a/t	t	W
8177410	43640	5	150428440	a/g	g	R
8177411	43792	5	150428592	g/c	g	S
8177412	43797	5	150428597	c/t	t	Y
8177413	43964	5	150428764	c/g	g	S
870407	44297	5	150429097	c/t	a	R
870406	44311	5	150429111	c/t	g	R
6873202	44588	5	150429388	a/g	a	R
8177414	44775	5	150429575	c/t	c	Y
8177415	44921	5	150429721	c/t	c	Y
3805435	45006	5	150429806	g/a	t	Y
8177416	45098	5	150429898	t/c	c	Y
3792799	45185	5	150429985	c/g	c	S
3792798	45475	5	150430275	t/c	g	R
3828599	45506	5	150430306	t/c	a	R
8177417	45543	5	150430343	g/c	g	S
3792797	45601	5	150430401	t/g	a	M
8177418	45652	5	150430452	t/c	c	Y
8177419	45756	5	150430556	ag/g	g	N
8177420	45826	5	150430626	t/c	t	Y
8177421	45974	5	150430774	c/g	g	S
4958872	46044	5	150430844	t/c	c	Y
3792796	46200	5	150431000	c/g	g	S
8177422	46218	5	150431018	a/g	a	R
8177423	46221	5	150431021	c/t	c	Y
4958434	46280	5	150431080	c/t	a	R
8177424	46330	5	150431138	—/gagtcctgg	gagtcctgg	N
8177425	46583	5	150431383	t/c	c	Y
8177426	46650	5	150431450	a/g	a	R
8177427	46721	5	150431521	a/g	a	R
8177429	46808	5	150431608	g/c	g	S
6889737	47242	5	150432042	c/a	c	M
3792795	47512	5	150432312	g/a	c	Y
8177430	47600	5	150432400	t/c	c	Y
8177431	47706	5	150432506	a/g	g	R
4958873	47806	5	150432606	a/g	a	R
8177432	47978	5	150432778	t/g	t	K
8177433	48021	5	150432821	t/c	c	Y
8177434	48025	5	150432825	g/a	a	R
8177435	48093	5	150432893	t/g	g	K
3763011	48413	5	150433213	t/c	g	R
8177436	48933	5	150433733	c/t	t	Y
8177437	49097	5	150433897	c/g	g	S
4958874	49105	5	150433905	t/c	t	Y
8177439	49570	5	150434370	g/a	g	R
8177440	49591	5	150434391	c/t	c	Y
8177441	49704	5	150434504	g/c	g	S
8177442	49705	5	150434505	a/t	a	W
8177443	49798	5	150434598	c/t	c	Y
869975	50082	5	150434882	a/g	g	R
869976	50147	5	150434947	g/a	a	R
8177444	50356	5	150435156	a/t	a	W
8177445	50725	5	150435525	t/c	t	Y
7721469	50968	5	150435768	c/a	a	M
8177446	51029	5	150435829	a/c	a	M
7704191	51086	5	150435886	t/c	t	Y
8177447	51166	5	150435966	t/c	t	Y
11548	51493	5	150436293	c/t	c	Y
2230303	51539	5	150436339	g/t	t	K
7722386	51562	5	150436362	g/a	a	R
8177448	51645	5	150436445	g/a	g	R
8177449	51649	5	150436449	t/c	c	Y
2070593	51650	5	150436450	t/c	g	R
8177450	51656	5	150436456	g/a	a	R
8177451	51657	5	150436457	t/c	c	Y
8177452	52009	5	150436809	g/a	a	R
8177453	52143	5	150436943	a/c	c	M
8177454	52349	5	150437149	g/c	g	S
3763010	52421	5	150437221	c/t	c	Y
8177455	52532	5	150437332	a/g	g	R
8177456	52682	5	150437482	a/g	g	R
736775	53058	5	150437858	t/c	t	Y
2277940	53187	5	150437987	t/c	t	Y
8177458	53377	5	150438177	g/a	g	R
8177834	53699	5	150438499	g/a	c	Y
3924	53845	5	150438645	a/g	g	R
2233312	53920	5	150438720	a/g	c	Y
2233311	53929	5	150438729	t/g	c	M
2233310	55473	5	150440273	t/c	g	R
2233309	55690	5	150440490	a/g	c	Y
4958875	55850	5	150440650	a/g	g	R
2233308	56761	5	150441561	c/t	g	R
2233307	56840	5	150441640	c/g	c	S
2233306	57000	5	150441800	c/t	g	R
2233305	57116	5	150441916	t/g	a	M
2233304	58419	5	150443219	t/g	c	M
2233303	58420	5	150443220	c/t	g	R
2233302	58808	5	150443608	g/c	c	S
2287719	58906	5	150443706	a/g	g	R
2287720	59048	5	150443848	c/t	c	Y
7727034	59187	5	150443987	c/g	c	S
7727250	59361	5	150444161	c/t	c	Y
7709800	61218	5	150446018	g/a	g	R
3840312	61700	5	150446500	ag/a	a	N
2287721	62290	5	150447090	g/a	g	R
6875293	62596	5	150447396	t/c	c	Y
3805434	64049	5	150448849	g/c	g	S
2080982	66077	5	150450877	g/t	t	K
2080983	66079	5	150450879	g/t	g	K
2287722	66086	5	150450886	t/c	c	Y
2233301	66115	5	150450915	t/g	c	M
2233300	66150	5	150450950	c/g	c	S
4958876	66475	5	150451275	c/a	a	M
2233299	69177	5	150453977	a/g	g	R
2233298	69210	5	150454010	a/g	c	Y
2287723	69312	5	150454112	t/g	t	K
2161359	70244	5	150455044	a/g	g	R
7734456	70882	5	150455682	g/c	g	S
4292439	71905	5	150456705	t/c	c	Y
4958878	72294	5	150457094	a/t	a	W
6862024	72581	5	150457381	a/g	g	R
3834819	72786	5	150457589	—/ca	ca	N
2233297	72950	5	150457750	g/a	t	Y
2233296	73106	5	150457906	g/a	c	Y
2233295	73162	5	150457962	t/c	g	R
2233294	73273	5	150458073	g/t	a	M
7713028	74131	5	150458931	g/a	g	R
7713223	74406	5	150459206	t/c	c	Y
7713567	74665	5	150459465	c/t	c	Y
888989	74740	5	150459540	t/c	c	Y
2233293	75382	5	150460182	a/g	c	Y
3749657	75400	5	150460200	g/t	c	M
2233292	75460	5	150460260	g/a	c	Y
2112635	75863	5	150460663	c/t	c	Y
871269	76098	5	150460898	t/c	c	Y
3792794	78432	5	150463232	a/g	c	Y
6579837	78604	5	150463404	g/t	g	K
3805433	79190	5	150463990	c/g	c	S
5872186	79870	5	150464670	—/a	a	N
2233291	79928	5	150464728	g/c	c	S
2233290	80213	5	150465013	g/c	g	S
2233289	80227	5	150465027	c/t	g	R
4958435	81994	5	150466794	t/g	g	K
4958880	82187	5	150466987	c/a	c	M
1422673	82698	5	150467498	t/c	c	Y
2042234	82841	5	150467641	a/g	a	R
3805432	83214	5	150468014	a/g	c	Y
3805431	83249	5	150468049	t/c	g	R
2233288	83485	5	150468285	t/c	g	R
2233287	83807	5	150468607	c/t	g	R
3815720	83907	5	150468707	g/a	g	R
3792792	84216	5	150469016	a/g	t	Y
3792791	84656	5	150469456	a/g	c	Y
2303018	85448	5	150470248	g/a	g	R
3792790	85881	5	150470681	g/t	a	M
4958436	86539	5	150471339	t/c	t	Y
2233286	86796	5	150471596	t/c	g	R
2233285	87057	5	150471857	g/a	c	Y
7732451	87922	5	150472722	t/c	a	R
2233284	88098	5	150472898	t/c	g	R
1422674	89319	5	150474119	g/t	t	K
3792789	89678	5	150474478	c/t	g	R
4562032	90026	5	150474826	c/a	c	M
6865077	90033	5	150474833	g/a	g	R
1559126	90114	5	150474914	c/g	c	S
3792788	90326	5	150475126	t/c	g	R
1559127	90463	5	150475263	t/c	t	Y
3792786	90548	5	150475348	a/g	t	Y
6880110	90800	5	150475600	a/g	a	R
6861227	90838	5	150475638	g/t	t	K
3805430	91400	5	150476200	c/g	c	S
1862364	92086	5	150476886	a/g	a	R
4958881	93946	5	150478746	t/c	t	Y
3792785	95360	5	150480160	a/g	t	Y
6869605	96576	5	150481376	a/c	a	M
6870205	96721	5	150481521	a/g	g	R
4246047	98316	5	150483116	t/a	t	W
4958882	98497	5	150483297	c/g	c	S
3792784	99382	5	150484182	c/t	a	R
3792783	99442	5	150484242	t/c	a	R
5872188	99764	5	150484565	—/ag	ag	N

Assay for Verifying and Allelotyping SNPs

The methods used to verify and allelotype proximal SNPs of Table 24 are the same methods described in Examples 1 and 2 herein. The primers and probes used in these assays are provided in Table 25 and Table 26, respectively.

TABLE 25
DbSNP
rs#	Forward PCR primer	Reverse PCR primer
1478398	TTCCCTCCCTTCCTTTCTTC	AAGATTACCCCACTGCACTC
1478397	CACATACTTGTCTGAGGATC	AGCACTCAGAATACTACCTG
1160114	AGCTGGATTCAGAGCCTTTG	TCTGCTATCACCTTGTTCCC
1160113	TCTGCTATCACCTTGTTCCC	AGCTGGATTCAGAGCCTTTG
1382323	AGAAGGACAGTTGCAACAGG	GTTCCTCATCCAGTCCTTAC
1160112	GTTCCTCATCCAGTCCTTAC	AGAAGGACAGTTGCAACAGG
7709870	TTATGACTCATAACTTTTTG	CTTTCTTGAAATGCTCAGGG
7710643	ATAAAGTCTGGGCTCAGATG	ATAGACCAGGGAACTGAGAC
7730467	TGTGGCATATCTTCTATTGC	AATGTGGCTGTTATGGTAGG
6579829	GGAGGCTTTTTCACATTCAC	AATGTGGCTGTTATGGTAGG
6579830	TGAGTATGGATTCTCAGGTG	ACCATAAGAGAGAAAAGCCC
6579831	GTGTGTGATGAATATGGGAG	TTTCTCTCCTGTGTGTATTC
6896232	GTCACACCTCTTTGTACATC	CACTGAAAGCTCTCTCACAC
1351131	AAGTGTGAGAGAGCTTTCAG	GTATAAGGTTTCTCTCTAGTG
1038074	TCATATCTTGTGGGTTGTCC	TTCACTGTGGACCACAAAGG
1478396	CATGGCCTCTTACAAGACTC	GGGTAATTGTACCCACCAAG
6880512	GAGGTATTGGTGATTAGGGC	TAAAAAGGAATTCTTGGTGG
4958858	GCAAGTGAGATCTTGGTAGG	CAGACTGGCAGGAGTACATT
4958431	CAGAGGTGTCACTTCAGGAA	CCATCTTGCTTCTTTCCCTG
4958432	TGCCTTAATTCATGCAACCC	TTATATCCAGGGAAAGAAGC
6898463	CAAATCTACTTTTGAGTACC	GACTCATCAAATGGAGAATG
4958859	GTGGTATAACTCCTCAGAAG	ATGATGAAGTTGTCTTCAGG
4130064	AATTCCTCACATTAACCCAG	CCACAGCAAAGACAAAAGGG
4130065	CCACAGCAAAGACAAAAGGG	TCCTCACATTAACCCAGGAG
4133119	AGTGAGGTATAATGCCAGCC	AAAATCTCCCTGTGTCTGCC
4958860	TATGCGGTGGAGAAGAAAGG	TCAACCCGGACAACAAAACC
4958861	ACTGGGATTTTCTGGTGAAG	ATGGGTGAGTCTCCCTTTAC
4437356	AGGTTTCTGGGTTTTCTCTC	ATGGTAGTGTGTTTGTTTGC
4958868	TTGTTGGCCTTGTTCATGTC	CCTGTAATCCCTGCACTTTG
1478400	TGGACTCCTGTGGGATAATG	CAAGACTTCCAAGTGCATCC
6889375	TACCTGTCCACAGAAATAGC	CCTGAGACCCTATGACTTTA
1600159	GGTTCATGTGATTTGGAAGC	TGACTCTCCTAGTTCTTCAG
6875892	GGGACTTCTCAGCTTCCAAA	AGGGTTCTCCAGAGAAACAG
4608909	CTGATAGAGGTTTATCTCAG	TCTAGCCAAACTCCTATGTG
	C
2345000	TAACAAGCCCCTAACTAGTC	TCAGGTGAAACCAACTGAAG
4516840	GTGCAGTGGATCCTTTTTCC	CTAGACTCTAGGTAGGACAC
2054440	CCCTTGAATGAGATGGAGAC	TGGGAAAGGAAGGGAATGTC
707141	ACAGTGCTAAGCACTTTCCC	ACAGTAGACACACAGGATAA
707142	AGCCTGGCTTCTTCGTCTAC	ATTTCCACCCTGGGCACTTC
841236	ATGTGTGCATGAGAGGAGAG	CCAAAAAGGAGGAATGTGGG
707143	CTTTTAAAGCCAGATGGACC	CCACCCTGTTCTTACTGTAG
707144	ACTGCACCAGACGCTTTCAC	TTGGGCAAGGCGTGTTTCCT
6869405	TCTCTCATCATGGCTTGTGG	CTGGCCAAGCGATAACACTA
707145	TAAAGTCTGGGGAGGTCAGG	AGTGGCTGCTTTCGGAACAG
707146	GCAACAAGAGCGAAACTCTG	AAATGGGCAACATGGCAAAG
707148	ACCTTTCATATACAGCTGGG	CTGTGTTCCAGCATCATCTG
707150	ACTGATCTGGGCTAGGAATC	AAGGACAGCCAATTAACACG
5872184	TAAAATGTTGTTTACCAGGG	AAGGATGCCTATGTGACCAG
3763015	GCCAAATATTGTTTTCCTTG	ACATCGACCCTTTGTTGTAC
2042235	TCCTTCTCTGATCTCAGTTG	TTAGGAATGAAAAGCCACAG
3763013	AGAGACCTGAGATGCTACCC	ACAGGTGGACAACTGAGATC
2042236	AAAGGTGTTTCCAGCAGATA	AAAATGTAGAGAGATGCCCG
1946234	AACAAGTCATCTAGCCAGAG	CTGGCTTAGAAGCAGAAAAT
1946235	CCTGGCTTAGAAGCAGAAAA	GGCACTGGAGATCACTAATC
1946236	GCACTGGAGATCACTAATC	CAAACCACCTGGCTTAGAAG
8177402	TATCTGTGGCCAAACCACCT	CACAGGGAGCAAATTTATT
8177403	TTTCCTTGAGTCTTTGGGTC	GCCTGTAGAAAAAGGTTAGC
8177404	ATTAGGACTGTAGGGACAGA	GCTAACCTTTTTCTACAGGC
8177405	ATTAGGACTGTAGGGACAGA	TTTTCTACAGGCATGTCAGC
8177406	ATGTCAGCTGTGATCCTTAT	GAAAACCCCATTCTGGGTAG
8177407	TGATGGAAGTCTCCATTCTG	AATTTCCAGAGGACCATCGC
8177408	GGGCTAATAGCTCCCTAGAA	CGCCAGGTGTTTTTAAAACC
8177409	ATGCTTCCCAGAATGGAGAC	TCCATTACAGCCAGGGCAAG
6888961	TCCTCTTTTGGCTCCAAAGT	TTCCATCAGTTCTAGGGAGC
8177410	TCCTTTCGCACTTTGGAGCC	ATAAGCAAATCTGGGCAGAG
8177411	TGTGACCAATCCGCGGCCAA	TGAAATCCCAGCCGCCTAGC
8177412	TGAAATCCCAGCCGCCTAG	TTTCAAGCCCTCGGGTGTGAC
8177413	TGCAGGCGTCCTGCCTGCT	TCACTCACCTTCGACTTCTC
870407	GTGACATAGATGTAGCAAGG	AGAATGACTAAGGGAGGAAG
870406	CGGAGGTGACATAGATGTAG	AGAATGACTAAGGGAGGAAG
6873202	TAGAAATGGCCACTTGGATT	AGTGTTCTGCACTGTCACTC
8177414	GGGATGCTTGGAGAAGCTGA	AATGAGGACAGTGGGTCCTG
8177415	CCTCAGTCTATTTCCAGCAG	ATAAGCATGCTGGGTCATCC
3805435	GACAGTATGTACTGGGGTGG	TTTGAGTCTATCCCTACACC
8177416	GAAGACCCAGAGGATTTTAG	AGGGATAGACTCAAAGCGAG
3792799	GGACAGAATTTTGGAAACGG	CTAAAATCCTCTGGGTCTTC
3792798	ATTTTCCAGGTCAGGAATGG	CCAAAACTGAAGGTTGGGAC
3828599	AGTCAGTCCCAACCTTCAGT	CAGGGCCCAATTGTATCTTC
8177417	AGTTATTCATGGAGTCCACG	TTTTGGAAGGGTCAAAGAAG
3792797	GAGTCCATTTATCCTTCCCC	ACGTGGACTCCATGAATAAC
8177418	AAGTCCTGACTGGTTGTATG	CTCAGAGAGAATAGGATGGG
8177419	ATGCCCATCTCTGCTCCCAA	TCCTAACCCAGAGCTAAGAC
8177420	ATTCCATTCTCCACATGCCC	TAAGAGTTCAGATGTCTTAG
8177421	ATTGAAAGGGAGATGCTAGG	TTGTAGAGACAAGGAGCGTG
4958872	AGTCACCTAGAAACCAGCAG	ACAAAAGGACTGCAGAAGCC
3792796	CAATATGATGCTGTGAGAAG	ATCTTACAATGGAGGAGCTG
8177422	ATCTTACAATGGAGGAGCTG	TATGATGCTGTGAGAAGTGG
8177423	ATCTTACAATGGAGGAGCTG	TATGATGCTGTGAGAAGTGG
4958434	CTGCTGGTCTTGTGTCTTTC	AGACCACTTCTCACAGCATC
8177424	GGCTTGAGAGTTGGATGATG	GGAGAGGAGAAAGACACAAG
8177425	AGAGCAAAACAGGCCTGGAG	ACCCATGGGCCACCTTCTGA
8177426	ATTTCGTTCCAAGAGACTCC	ATATGTCCAGGGAGATAAGC
8177427	TTATCTCCCTGGACATATGC	GGCAAGGAGAACCCTATTTC
8177429	CAGGTTGTCCTAATAATGGG	ATGAAATAGGGTTCTCCTTG
6889737	CATGACAGAGGCAGCTCTTC	TGAAGGGAGTGATATATGCT
3792795	GGCCACAAAGGACCTTAAAG	TGACACAATTTGGCAGGTGG
8177430	AGTAGGATGTGGCCTATGAG	ATTGTGTCAATGATGCAATG
8177431	CATGATCCTATCAAGCCATC	GACCTGAGTTTCCTCATTTA
4958873	GCCAGTCCAAGACCACACAG	TGGGAGACCTGGGCTCCAT
8177432	CCCGGAACATTAACACATGC	TTACCAAATAGGGAAGCAGG
8177433	GGACAGGAAAGATGAAGCTG	ATGCAGGTACTGTTGCTTTT
8177434	GGACAGGAAAGATGAAGCTG	ATGCAGGTACTGTTGCTTTT
8177435	ATTGGGAGCCTCTGAATCAC	GGCCAGTGTGAAGGAAAAAC
3763011	GTGCTTTTTAAGCACTTAAT	CTCCTAATTCCTTGTTTAAG
8177436	CACTGTGTTCCGTGGTTTTG	CCTAGAACATGTCCAAAGGC
8177437	GAGATGGTAACTGAAGCTCC	TGCCAAGAGAAGCACCAAAG
4958874	TGCCAAGAGAAGCACCAAAG	GAGATGGTAACTGAAGCTCC
8177439	AGAGAAGCATCCCAGAAAAA	TCCATGCTCTGCCTGGCAAT
8177440	GCAATCACAGGCGAATTCCC	GCATCCCAGAAAAAGCACTC
8177441	TCCCCAGTTTCCTCAGTGTC	AACAGAGCAGAGTGCGTTTG
8177442	TCCCCAGTTTCCTCAGTGTC	AACAGAGCAGAGTGCGTTTG
8177443	TGCCAAAGATTATGGGAGGG	GAGCAAGAACTATCCATCCC
869975	CTTTCTCGGGCCTCAGTAGT	CTGTCTCATCCACACCACTC
869976	AGTGCATTCAGTTCTGGGCC	AGTGGTGTGGATGAGACAGG
8177444	AGGAGAGTAGTTCTTGGTGG	AGAAAGCTCCTCTCACATGG
8177445	TTCTCTCCATTGACATCCCC	TCAACAGGTATGTCCGACCA
7721469	ACTATTGTTCCAACTAGAGG	ATGTTCACTCTGGGTCCTTC
8177446	AGTTTGGGAGTTAGAAGACT	CAGAAGGACCCAGAGTGAAC
7704191	TTCCTGGGGAATAGCCTGGC	CGCTGGCAGTCTTCTAACTC
	C
8177447	TGGGAGGACAGGAGTTCTAG	GCCTCAAGCAAGGTTGACAC
11548	TGTTCAGGAAGAAATCCGTG	GGGGCCTTGAGTGATAGGA
	T
2230303	TGCCTGGCAGTACACAGAAC	ACTATCTACCCATCACAGAC
7722386	CCTGTAGGCATGTGTGTAAA	GCACAAATGGATGCATACAG
8177448	CAGATGGTACACATTCCCAG	GCCTACAGGTATGCGTGATT
8177449	CAGATGGTACACATTCCCAG	GCCTACAGGTATGCGTGATT
2070593	ACACATGCCTACAGGTATGC	CAGATGGTACACATTCCCAG
8177450	CAGGCACACAGATGGTACAC	GCCTACAGGTATGCGTGATT
8177451	CAGGCACACAGATGGTACAC	GCCTACAGGTATGCGTGATT
8177452	TGGCCCTTGCTGTCACATCT	TACATCCCCACCCCACAGTT
8177453	AGGGGTGGCATCCCTGCCCA	TGAGGGGCCAGCCCTTAGTG
8177454	TGCACCCACTGTAAAAAGT	AAGTGAGGAGGAGAAGGTGG
3763010	AGTGGGTGCAAAGGTAATTG	TGATCTCAGGGAGAATTGTG
8177455	ATGTAGCCCTATTAGGGGTG	AGCTAACTATCAGGTTGTTG
8177456	CCCGGGAGTTTGAGGCTATA	AGTGCAGTGGCTCTTCACAG
736775	TACTCTTGAGTAATGGTGAC	TGGCCTAGGCTCCTTCCACT
2277940	CGAATCATGGACATAAATCC	TCAGGGAAAGGAAGAAAAGG
8177458	ACAGTAGCCTTGCTGAAGCC	CAGCTTCAGGCTGGCGCCC
8177834	TCTTAGGATTGCTGCTCCTG	GAAAAACTCCTTCCCTGCTG
3924	TCCCCAGTCCTGTAAACAGC	GAGCCTCAGCTGGATGAGAG
2233312	ATTGTGTCATTTGGCTCCAC	CACACCGTGCAAGTGGCTTC
2233311	TTGTGTCATTTGGCTCCACC	CGTGCAAGTGGCTTCTAGTT
2233310	CACTGTTTTGGAAAGCTGGG	TTATCTGCCAGCATTGCCTG
2233309	ATTTAGTATTGTCCTGGCAC	TATTCCGGCTGCGACAAAAC
4958875	GGGATAACTTGCTGAAGGTC	GCTTAAGAGATTGGCTCTGG
2233308	AGACACCTTCCAGAGAGGA	AGAGCCAGGCCCTGGACAC
2233307	TCTTCCATCTGGTGAGTCTG	TCCTCTCTGGAAGGTGTCTG
2233306	CCACCCAGAACATCTCTGC	ATGGTGTGGCACCATGGCTG
2233305	CTAGCCTTCAGAGAGCTAAC	TCTGGCTTTGGCCTGCAACA
2233304	TGGCCCAGAGGGAAGCATCA	CCTGGATCCAGGGCCTAAC
2233303	TGGCCCAGAGGGAAGCATCA	CCTGGATCCAGGGCCTAAC
2233302	ACCAGGGCCCTCCAATCCAT	AAATGCCCAGGTAAGAGTGA
2287719	CTGCAGCTTCTCCACTTGCT	TCGTGAGCGCATGAATGAGG
2287720	AGGGAGGGTGAAGAGAGGGA	ATCTGTGACCCCAGCAGGAG
7727034	AGAGTAGTTCTGTGTCCCTC	TGGTTGATGGCTTTCAACGT
7727250	TTGGATCTGCTGGTTCTGAG	ATGTGAAACTACAGGCTAAG
7709800	TTGCTCATGACCTAGGCATC	CTTTATCTTCCTACTCCACC
3840312	GAAATGTGCTGGAAAGCAGG	CCTCTCCAGTTCAGTTTTGC
2287721	CCTTGAGTCCAGCTGCAATG	GGGTTACCCTTCCTAAGCTG
6875293	ATGGACATTACTGTACCGAC	TGGCCCAGCCCTTACGTTCT
3805434	AAATAAGGCCCCTGGTGTCC	GGGCATGGTCTTTTGCCTAG
2080982	CTGCCTCTTTAGCAGCTTTG	AACTTCTCCTCTGGGCCATA
2080983	CTGCCTCTTTAGCAGCTTTG	AACTTCTCCTCTGGGCCATA
2287722	CTGCCTCTTTAGCAGCTTTG	AACTTCTCCTCTGGGCCATA
2233301	CACAGGGCATGCCTGGTCTT	CCACTCCCCAAGGTTCAAAG
2233300	TGAACAAGCAGTGGGACCAG	AAGACCAGGCATGCCCTGTG
4958876	TTTTACCTTGCCAGGAAAGC	CAAAGAGGTGTGTGTGTTCC
2233299	CCAGCATCTTCACCTTCTTC	TTTTGGTGAAGGCTCCTCTG
2233298	TTTTGGTGAAGGCTCCTCTG	AGCATCTTCACCTTCTTCTC
2287723	AATTATCTCACCCAGTCTCG	TGGGAGGAATAGGGAGACAG
2161359	ACAGCTCTTCCTTACAAAAG	TTAGTGACCATTGGTGGATC
7734456	AATAGTCTAGAGAGTTCCCC	CCTGGGTTCTATAAGATGTTG
4292439	CCCTGGACAGGTCTCTTTAC	AGGAAGAGCATCTGGCTCGG
4958878	AGAGTTTACCCAGGACATGC	TAGAATGGAATACTAGTCCC
6862024	GTGCTGGGACTAGAGGATAG	TGTTGGCTAGTGTCTGTGTG
3834819	TGGGAACCACTCTCTAAGAC	GTGTTGAGTCGTTTGCTCAG
2233297	ATGAAGCCACAGTGACTACC	GTGGTAGAAGGTTCTAGCTG
2233296	AACCAAGGATGCTACAGAAG	ATGGAAGGGACAGGCAAGAA
2233295	TTGCCTGTCCCTTCCATCTT	ATCCCAGGGAGGAAAATTTG
2233294	TGGGATGGAGGTAAACAGAG	CAACTCATGCGATAGTGAGC
7713028	CTGGTGAAATACATCACCAC	TTGAGTAGCTGGGACTACAG
7713223	CTTTTGGATGGCCAAGGATG	CCAGCTTCAGTTTCTGAATC
7713567	AGCTGGTACCCACCAGATGA	ATCCTGTGAGTGGCCACAATT
888989	CAGGGACTTGATTGGAGTCT	ACTCACAGGATGGAGAGCAA
2233293	TCAGAGAAGGGGTGAGGAGT	AACCCAGAAATCAGTGCTGC
3749657	AGAAATCAGTGCTGCACACC	TAAGTTCAGGCCCCTCAGAGA
2233292	TGAACTTACCGCAGCCTCTC	CCAAGTTGGATAAGGGCCTG
2112635	TCCATCCCTTCTCCTTCAT	GGACTTCTCTGAGGAGATGG
871269	TTCCACAAACTTTGAGCTGG	TAATTAATTCCTGAGCTCCC
3792794	TCTGCTAGAAGGTAAGCTTG	AGTGTGTATGAAGTGCCACG
6579837	ATAGATCCTAGGCCCCTTAA	AGGAAATAAACAAGGAGGGG
	A
3805433	CTTAGCCTTGTGGGCTTTAG	AAGCCTCCGTTTTGCCATCT
5872186	TCCCGTATTTCCCCATTTCC	GCTTTTTGTTCAGGGTGATG
2233291	CCTTATCCTGTTCATCACCC	AATCCACTGGTACCATGGGG
2233290	CTGCAGGTGCAGCATCAGG	TACTCAGGCCTTGGTGAAGA
2233289	TCAGGCCTTGGTGAAGAGTG	ATCAGGTTGCCGTCCTCAC
4958435	AAGTTCTGCTCATCCTTCCC	GATCTCTGAGGCTCCCTGTA
4958880	TCTGGAGGAATGGCAATGAC	ATTTCCTCTTGTTAGAATGC
1422673	ATGCTTGTGTTCACTGGTTG	CATGCTAGTTAGCCAGACAG
2042234	GCACCTATTGCTCGCATAAC	TCTACCTTCTCCATTCTCCC
3805432	AAGGCCCAGGGCCCTGTAAG	AACCTCCTTCCTGTGTGCTCC
	A
3805431	AAATCACCTGTCTTACAGGG	TTTGGGTCTGTGGGCTTCCA
2233288	ATATGTGAGCGAGAAGCACC	ACAGAACCTGTTCAGATCCC
2233287	ATGACAGGCTTGTCGAGAAT	TTTTGAGTACACAGGGACCG
3815720	TGCTGGTTCAAGGTCAGCTA	TAATGTGAGGTCATATCCCC
3792792	TTCTCAGATCAGTTCACTCC	AACACAGATGGCAGCTGTTAC
3792791	GATTGCATAGTTCAGCATCC	CATTACCCGTGTAACCTCAG
2303018	CAAATGTCACAGCATCTCCC	TAAAACTTACACTGGATGGA
3792790	CATAAAAGCCCCATATCCCC	AGGGAGGGCCAAGTCTCAGT
4958436	GCCTTGGGGTCTTTATCAGC	ATATGCCAGCTCCAAGTGAC
2233286	ATGAATGGAAGGGTAGCCTG	ATCCTTCACAGTGAACTCCC
2233285	TTCCAAAAGCTCCCCTAGAG	ACGGGGAGAAGCAGCACAC
7732451	CATCTCTCGTGCCCCAGAAA	GCTGGAAGGCCCAGTTAATG
2233284	TACATGGTGCATAGAGACAG	ATATGCCTCTGCCCAAGTCA
1422674	TGCTGATAATTCCTCAGCAC	GTCCATTCACTCATCCATTC
3792789	GAGGATTCCTGATGAAACAG	TTCACTGGGGACCTCAGAAG
4562032	ATTTTACAAGGGAGAAACTG	CTGACTTTGCATCTTGGCTC
6865077	CTGACTTTGCATCTTGGCTC	ATTTTACAAGGGAGAAACTG
1559126	CACCATACTCACACAGCCAG	ATGGTGCTGCCCTACAAAGA
3792788	CCCTTCTCCCAAAGACTGAC	AGTGTGGGTAGCTGTCCTTT
1559127	ACTGTTTCTTAGTTCGGTGG	GGTGACTTGTTTTTAACCTG
3792786	TGAGGACAGAGGCAAGCAGA	AACAGTGTGGGGGCTGCACTT
6880110	TCAAGAACAGTGAAACAGAG	TGGTGGACAAGAACCAGGAC
6861227	CATGCCTTCATGTGGTGTTG	AGCAGTGTCCTGGTTCTTGT
3805430	TCCAGAGAGGGTTTTCCCAC	GGGAGAAAGAAAGGCAGTGG
1862364	AATCCCATGGCACACCACAG	TGTAGTTTGTGGAACCAGGC
4958881	GGACTGGAGGTCATCATAAC	TTCCACCCAAGGATGAAAGG
3792785	TTTGTTCTACCACTAACCAG	CTACATGGATGAGGAAGCAG
6869605	TTTGTCCAGCTTCTGTGTAC	AGGTGTGAGTTACCTAAGGG
6870205	GAATCATCAAGAAGGGACTG	ACCCCATTCTTTTTGATGGC
4246047	AAGCTGGTCTCAAACTCCTG	TGTTATTTAGGCTGAGCGTG
4958882	TTGTTGGTGGCTGATGAAGG	GCTCACTCATCTGGTTTAAG
3792784	ATGATTTTCCCATGACTGGC	AAACAGCCAGCTGCTGCTCA
3792783	AATCTGAAGAGTGACTGTCC	AAAGACCTTGGCGGCCACCAT
5872188	CCTTTGCCGACTTTGGTTTC	AGACTTGGAAGAGAATGCCC

TABLE 26
dbSNP	Extend	Term
rs#	Primer	Mix
1478398	TGAGACAGGGCCTCACTCT	ACT
1478397	GTCTGAGGATCAGTAATAATAC	ACT
1160114	CTTAATTGCAATGCCTCTT	ACT
1160113	CTTGTTCCCTACCTAGC	ACT
1382323	CTGACCAGGGTAACAAC	ACT
1160112	TCTCCAATCACCGTTGT	ACG
7709870	CCTACAAGAAATATCAAGGC	ACG
7710643	GAGCATTACAGTTGTCAC	CGT
7730467	TTGCCACTAAATAAATCCAA	ACT
6579829	CATTCACTATGCTCTTTTTTT	ACT
6579830	ATTCCCTGCTGAAGGTT	ACG
6579831	AATATGGGAGAGCCTTC	CGT
6896232	AATACACACAGGAGAGAAA	CGT
1351131	GAGAGCTTTCAGTGAAGT	ACT
1038074	GTGGGTTGTCCACATAT	ACG
1478396	CAAGACTCACTGCAATTTA	ACT
6880512	GGGCTTCAACATATGAAT	ACG
4958858	GAAGATGAAGGCCTGAG	ACT
4958431	CTTCAGGAAAATCCGGC	ACT
4958432	AAATACAGTCAGCCCCC	ACT
6898463	CTTCATTTCAGGTATTCTACT	ACT
4958859	TCAGAAGAGGAGAGCAC	ACT
4130064	CCTTCCAAAATTTCCCT	ACG
4130065	GGGAAATTTTGGAAGGT	ACT
4133119	GCCAAGTATCCTGTATCAA	ACT
4958860	AGGGCAACAATAAGGGA	ACT
4958861	AAGGAGCAGAAGCATGA	ACT
4437356	CTGAGCAGCCCTAGTGA	CGT
4958868	CTCCTGACCTCGTGATC	ACG
1478400	TGGGTGAAAAAATTGGTA	ACT
6889375	CTGCTGATGATGTAGGAAT	ACT
1600159	GAAGCTGAGAAGTCCCG	ACT
6875892	CCAATTCATTATAGGAAATTTTTT	CGT
4608909	GGTTTATCTCAGCATTGTTTAT	ACT
2345000	CTAGTCTCCTTGTTCTCTTA	ACT
4516840	TCCTTTTTCCACCTTGC	CGT
2054440	GTAAACATCCAAAAGCATTTC	ACT
707141	ACATCCTGAGATGAAGG	ACG
707142	CAGGCAGCAGCAGCACA	ACT
841236	ACCTTCTCTGAGGGTATTCTA	ACT
707143	CCCTTAGAGGTCATCTGGTC	ACG
707144	CCATACAAAATCCCCTTG	ACT
6869405	TGACTGGGACCCAGGCT	ACT
707145	GGCCCAGCAGCCAGAAG	ACG
707146	GAAAAGAAAGGCTAAGTGA	ACG
707148	ACTGAGGCCCAGAGAGG	CGT
707150	GTCTGCGCCACCTTCCC	CGT
5872184	GGAGACTCAATGCCAGG	ACT
3763015	CTGTGCTGGTGCTGGTG	ACG
2042235	ATCTCAGTTGTCCACCT	ACT
3763013	TACCCCTGGATTGCTAC	ACT
2042236	AGGCACCTAGGGTCACA	ACG
1946234	CACTGGAGATCACTAATC	CGT
1946235	CCTCAGTTTCCTCAGCT	ACT
1946236	ACACAGGGAGCAAATTT	CGT
8177402	CCAAACCACCTGGCTTA	ACT
8177403	GTCTTTGGGTCTTCATTT	ACG
8177404	TCCTCCCCATCATAAGG	ACT
8177405	TTCCTCCCCATCATAAG	ACT
8177406	CTCTGTCCCTACAGTCC	ACG
8177407	GCATTTCTTATTCCTATCGAG	ACT
8177408	GCTCCCTAGAACTGATG	ACT
8177409	GAGACTTCCATCAGTTCT	CGT
6888961	CCATTACAGCCAGGGCA	CGT
8177410	CTTTGGAGCCAAAAGAG	ACT
8177411	GCGGCCAAGCCGAGACC	ACT
8177412	GATTGGCTGCAAGGGTC	ACG
8177413	TCCTGCCTGCTTTCCCT	ACT
870407	GACCCTCGAGGTGGCAG	ACG
870406	AGATGTAGCAAGGCGAC	ACG
6873202	CAGAGATCTGTGCTAGAA	ACT
8177414	TGTTCTCTGAAATTTCCTC	ACG
8177415	AGCAGGTGTGTGGCCCA	ACG
3805435	TACTGGGGTGGGCTCTG	ACG
8177416	TGCATGTTGGGAAGTTG	ACT
3792799	CGGAATCCTAGACTCATAA	ACT
3792798	ATAGACAGGCCAGCACC	ACT
3828599	CAACCTTCAGTTTTGGAA	ACT
8177417	ACGTTCTCCCCACTAGC	ACT
3792797	ATCCTTCCCCAGAGTGC	ACT
8177418	CTCACCCTAAAACTTTTCTA	ACT
8177419	CTGCTCCCAAGTTCTTA	ACT
8177420	ACATGCCCCATAACCCT	ACT
8177421	TCCCCAGTCCAACTTCA	ACT
4858872	GCAGTTACTAGGATCCC	ACT
3792796	TTGCTGTGTCTCTGATG	ACT
8177422	CATCAGAGACACAGCAA	ACT
8177423	CAGAGACACAGCAAAGC	ACG
4958434	TCTCCTCTCCCGTCTTAT	ACG
8177424	TGGATGATGGGACTCAG	ACG
8177425	GAACGAAATCTCATGTCAA	ACT
8177426	AGCAGATTCCCACAACC	ACT
8177427	GACTTGCTCAGGGCCAC	ACT
8177429	CCTAATAATGGGAACTTGTAAA	ACT
6889737	GGCAGCTCTTCTGCACT	CGT
3792795	TTCAAGGTTTCTCCTTTC	ACG
8177430	ATGAGCCTACTCTGCCC	ACT
8177431	CATGGTTGACCTGGTTG	ACT
4958873	ACAGGGAGTTAATGGCA	ACT
8177432	TGTTGCTTTTCCCTACC	ACT
8177433	GCTGTTGTTACCAAATAGG	ACT
8177434	GAAGCTGTTGTTACCAAA	ACG
8177435	GAATCACCATGTCCATAC	ACT
3763011	AAGCACTTAATATTAAGTACCC	ACT
8177436	TGTGAAGATGATTATATAAGCC	ACG
8177437	AAGCTCCATCTTGCTGA	ACT
4958874	TGGGGCTGAGGGAAAAT	ACT
8177439	TTTCACTTCCGGGAATT	ACG
8177440	TCCCGGAAGTGAAAGGA	ACG
8177441	TCAGTGTCCCCTGGTCT	ACT
8177442	CTCAGTGTCCCCTGGTC	CGT
8177443	GAGGGTTGTAACCTCAC	ACG
869975	GGGCCTCAGTAGTTCCAGC	ACT
869976	CCAAAGAGAAAGAGCAGA	ACG
8177444	GATAAATGTCCACCATGA	CGT
8177445	CTGGAAATTAGGGACAA	ACT
7721469	ACCCTGACTAGGGTCTC	CGT
8177446	GCTGTGGCTCTAGAAGA	ACT
7704191	ATAGCCTGGCCCTGAGA	ACT
8177447	AGTTCTAGAGCAGGGAT	ACT
11548	CACACTATCTACCCATCA	ACG
2230303	GTACACAGAACTGTATGC	CGT
7722386	ACCCACACCCACATGCC	ACG
8177448	CATAGGTAGACACGTGG	ACG
8177449	AAGACACATAGGTAGACAC	ACT
2070593	GCATGGGTGTACAGCCAC	ACT
8177450	CCAGAAAGACACATAGG	ACG
8177451	CCCAGAAAGACACATAG	ACT
8177452	TGTCACATCTGCCTTGG	ACG
8177453	CCTGCCCAGGGGCCTTA	ACT
8177454	TCTTTTCTCATCCTCCC	ACT
3763010	AAAGGTAATTGCGGTTTT	ACG
8177455	GGTGGCATCTTCATGAG	ACT
8177456	TGAGGCTATAGTGTGCC	ACT
736775	TCCCTGAGGGTAGGGCA	ACT
2277940	CATGTTCATTTATGCGCT	ACT
8177458	GCCATGCCAGCCACGTC	ACG
8177834	CCTGGAGGCTTCTGCAA	ACG
3924	CTCAGTTCAGGGACTGGT	ACT
2233312	TGATCTCAGATTGCCAA	ACT
2233311	CAGAGCCAGCTGATCTCA	ACT
2233310	CCAAGAGCAGAAACTAAC	ACT
2233309	TGCTATTGTAATTTTTGGGT	ACT
4958875	CTGAAGGTCATCTAGCAA	ACT
2233308	CTTCTGAACTAAACAGCAC	ACG
2233307	GCTCCAGGGCACACACA	ACT
2233306	CTACCCCTACGCCTACC	ACG
2233305	GATGAGATGGGTGTCCT	ACT
2233304	TCAGCCGGTCAGTCCTC	ACT
2233303	ATCAGCCGGTCAGTCCT	ACG
2233302	TCCATGCCCCCTCTCCC	ACT
2287719	CTCCACTTGCTTCTTCA	ACT
2287720	TTCTCCTCAGGCCCAGA	ACG
7727034	ACATTCTGGGCTTCAAG	ACT
7727250	AGAGACCCTACAAACTC	ACG
7709800	CCTAGGCATCTCCCTGT	ACG
3840312	CTAGAGCCGTTCCCACC	CGT
2287721	CCCATCACTGGCACCCC	ACG
8875293	GAGCATTGTGAAGTGATG	ACT
3805434	TGCTGACGGGAGGAACT	ACT
2080982	AACCTTGGGGAGTGGCC	CGT
2080983	TGAACCTTGGGGAGTGG	CGT
2287722	GCAGCTTTGAACCTTGG	ACT
2233301	TGCCTGGTCTTCATCTC	ACT
2233300	GGTAATGTGGGGTTCCT	ACT
4958876	CTAAGACATGGAAACTACAT	CGT
2233299	TCACCTTCTTCTCGGCTGC	ACT
2233298	CCCCAGGCTAGTGTGAC	ACT
2287723	AAGACCCCTCACCCAAG	ACT
2181359	CATTCCATTACTACAGTAATAACT	ACT
7734456	CCCTTCCTTTACTTCCC	ACT
4292439	CCCTTTAATCTCCACTC	ACT
4958878	CAGGACATGCAAATCTATT	CGT
6862024	CTAGAGGATAGGGGAGT	ACT
3834819	CTTGACAAACTGGAATGT	ACT
2233297	ATGGAGGTCAGGCCAAG	ACG
2233296	ACATACCTGCTGCTGTC	ACG
2233295	AGCCTGGCCGCCCAGAC	ACT
2233294	CCCTGAGTTAAGAAACCT	CGT
7713028	TACATCACCACCCCTCC	ACG
7713223	GGGGTTTTTATAGCCCTA	ACT
7713567	ACCAGATGACTCATCAC	ACG
888989	GATTGGAGTCTTACAACA	ACT
2233293	GGGGCTGCAGGAGGAGG	ACT
3749657	CCTCCTCCTGCAGCCCC	CGT
2233292	TCTCGGCAGCCTGATCC	ACG
2112635	CCCCAGCTGACTTCTAC	ACG
871269	CTGGCAGTGAACAAGAG	ACT
3792794	TGTTGTATTCACTGTTGC	ACT
6579837	AGGGGTCCTGCCAACCT	CGT
3805433	TGGGCTTTAGCAGCGGG	ACT
5872186	CCTCATCCCTCCCCACA	CGT
2233291	GTCTGCCCACAACACAG	ACT
2233290	GTTGCCGTCCTCACGGG	ACT
2233289	CCCTTTCCCTCTCCAGG	ACG
4958435	ATGGGAATCACAGGACA	ACT
4958880	GAATGGCAATGACTATAACC	CGT
1422673	TGTCAGTGCCCAGTGCA	ACT
2042234	CTCGCATAACAACAATTAAAG	ACT
3805432	CCTGTAAGACAGGTGATTT	ACT
3805431	GGGCCCTGGGCCTTCCT	ACT
2233288	CCCCAAGTGACTCCAGG	ACT
2233287	AATCCTGCTTTGTGATCT	ACG
3815720	AAGGTCAGCTAGTCCCA	ACG
3792792	GGGAAGGCCAGCACAGG	ACT
3792791	AGGTGCAGGGAAAGAAG	ACT
2303018	CAGCATCTCCCACAGCC	ACG
3792790	GCTTGGGAAGGTCATCA	CGT
4958436	TGTGTGCCAGGCATCGC	ACT
2233286	GATAAACGAGAGAATGTGG	ACT
2233285	GGAAGCAAAAGCATTTACT	ACG
7732451	GTGCCCCAGAAAGAGGAG	ACT
2233284	GTGAGGCAGGAGCCAGC	ACT
1422674	CACCCAGACTGGTGCCT	CGT
3792789	CCTTGGGAGGAAGGTGC	ACG
4562032	TAACTCAGCCACGATGA	CGT
6865077	GCATCTTGGCTCTTATTG	ACG
1559126	ACAGCCAGGACACAGAG	ACT
3792788	CAGATACAAGATGAATACACC	ACT
1559127	TCGGTGGAAACATCTGC	ACT
3792786	GACCCCCAATGTCTGCC	ACT
6880110	GCCACCTTGCATGACAA	ACT
6861227	GAGTTCCATTTAGGGTG	CGT
3805430	CAATAGGATATTTCTCCTGC	ACT
1862364	CCCCACCACATCTTCTC	ACT
4958881	GCAATGTGATATCATGGC	ACT
3792785	TCTCAGGCAAATGACTT	ACT
6869605	CTCTAGATTCCTAGATAGGG	ACT
6870205	ACAAACCACCATTCATTC	ACT
4246047	TGCTCTGCCTCCCAAAG	CGT
4958882	TGATGAAGGAGAAATTTCAA	ACT
3792784	TCACTCTTCAGATTGGAA	ACG
3792783	AAATCATCAAGAACTTCCC	ACT
5872188	GAATGAAAATGTTTCACTCT	ACT

Genetic Analysis

Allelotyping results are shown for female cases and controls in Table 27. The allele frequency for A2 allele is noted in the fifth and sixth columns for control pools and case pools, respectively, where “AF” is allele frequency. Some SNPs do not have an allele frequency disclosed because of failed assays.

TABLE 27
	Position in							Low BMD
dbSNP	SEQ ID	Chromo-some	A1/A2	Control AF	Case AF			Associated
rs#	NO: 5	Position	Allele	(High BMD)	(Low BMD)	p-Value	OR	Allele
1478398	231	150385031	A/G	A = 0.28	A = 0.34	0.0334	1.31	A
				G = 0.72	G = 0.66
1478397	330	150385130	T/C	T = 0.61	T = 0.67	0.0621	1.29	T
				C = 0.39	C = 0.33
1160114	582	150385382	G/C	G = 0.96	G = 0.92	0.0034	0.44	C
				C = 0.04	C = 0.08
1160113	589	150385389	A/G	A = 0.96	A = 0.93	0.0169	0.51	G
				G = 0.04	G = 0.07
1382323	1060	150385860	A/G	A = 0.67	A = 0.63	0.1288	0.83	G
				G = 0.33	G = 0.37
1160112	1066	150385866	G/A	G = 0.91	G = 0.85	0.0025	0.57	A
				A = 0.09	A = 0.15
7709870	1311	150386111	G/A	G = 0.11	G = 0.12	0.5796	1.11	G
				A = 0.89	A = 0.88
7710643	1556	150386356	G/T	G = 0.87	G = 0.86	0.7100	0.93	T
				T = 0.13	T = 0.14
7730467	1655	150386455	T/C	T = 1.00	T = 1.00
				C = 0.00	C = 0.00
6579829	1692	150386492	A/C	A = 0.92	A = 0.89	0.1423	0.74	C
				C = 0.08	C = 0.11
6579830	1802	150386602	G/A	G = 0.91	G = 0.89	0.4377	0.86	A
				A = 0.09	A = 0.11
6579831	2061	150386861	T/A	T = 0.31	T = 0.32	0.7948	1.04	T
				A = 0.69	A = 0.68
6896232	2112	150386912	T/A	T = 0.90	T = 0.89	0.7880	0.94	A
				A = 0.10	A = 0.11
1351131	2153	150386953	T/C	T = 0.51	T = 0.49	0.4152	0.91	C
				C = 0.49	C = 0.51
1038074	2667	150387467	C/T	C = 0.23	C = 0.27	0.1062	1.25	C
				T = 0.77	T = 0.73
1478396	3115	150387915	T/C	T = 0.92	T = 0.90	0.1123	0.71	C
				C = 0.08	C = 0.10
6880512	3186	150387986	G/A	G = 0.64	G = 0.60	0.2313	0.86	A
				A = 0.36	A = 0.40
4958858	5621	150390421	T/C	T = 0.06	T = 0.13	˜0.00001	2.54	T
				C = 0.94	A = 0.87
4958431	5735	150390535	T/G	T = 0.98	T = 0.95	0.0132	0.44	G
				G = 0.02	G = 0.05
4958432	5829	150390629	G/C	G = 0.86	G = 0.85	0.4357	0.87	C
				C = 0.14	C = 0.15
6898463	6658	150391458	A/C	A = 0.86	A = 0.82	0.0341	0.71	C
				C = 0.14	C = 0.18
4958859	7901	150392701	G/C	G = 0.95	G = 0.90	0.0030	0.48	C
				C = 0.05	C = 0.10
4130064	11447	150396247	G/A	G = 0.26	G = 0.30	0.1514	1.21	G
				A = 0.74	A = 0.70
4130065	11466	150396266	A/G	A = 0.16	A =
				G = 0.84	G =
4133119	11984	150396784	T/C	T = 0.95	T = 0.94	0.2216	0.72	C
				C = 0.05	C = 0.06
4958860	15803	150400603	T/G	T = 0.10	T = 0.17	0.0163	1.88	T
				G = 0.90	G = 0.83
4958861	16257	150401057	T/G	T = 0.13	T = 0.17	0.0581	1.38	T
				G = 0.87	G = 0.83
4437356	17604	150402404	C/A	C = 0.10	C =
				A = 0.90	A =
4958868	19762	150404562	C/T	C = 0.55	C = 0.53	0.6623	0.94	T
				T = 0.45	T = 0.47
1478400	22367	150407167	A/G	A = 0.96	A =0.94	0.0584	0.57	G
				G = 0.04	G = 0.06
6889375	22709	150407509	A/G	A = 0.59	A = 0.61	0.5378	1.09	A
				G = 0.41	G = 0.39
1600159	23631	150408431	G/C	G = 0.62	G = 0.61	0.7340	0.96	C
				C = 0.38	C = 0.39
6875892	23686	150408486	T/A	T = 1.00	T = 1.00
				A = 0.00	A = 0.00
4608909	25599	150410399	T/C	T = 0.98	T = 0.92	0.0008	0.27	C
				C = 0.02	C = 0.08
2345000	26973	150411773	A/C	A = 0.57	A = 0.55	0.5708	0.93	C
				C = 0.43	C = 0.45
4516840	28457	150413257	G/T	G = 0.00	G = 0.00
				T = 1.00	T = 1.00
2054440	28669	150413469	A/G	A = 0.62	A = 0.66	0.1835	1.18	A
				G = 0.38	G = 0.34
707141	29908	150414708	C/T	C = 0.45	C = 0.46	0.6490	1.06	C
				T = 0.55	T = 0.54
707142	30105	150414905	A/G	A = 0.54	A = 0.53	0.7349	0.96	G
				G = 0.46	G = 0.47
841236	30711	150415511	A/G	A = 0.55	A = 0.54	0.7731	0.97	G
				G = 0.45	G = 0.46
707143	30851	150415651	G/A	G = 0.64	G = 0.66	0.6049	1.07	G
				A = 0.36	A = 0.34
707144	31203	150416003	T/C	T = 0.69	T = 0.75	0.0163	1.38	T
				C = 0.31	C = 0.25
6869405	31446	150416246	A/C	A = 0.08	A = 0.09	0.5734	1.13	A
				C = 0.92	C = 0.91
707145	31638	150416438	C/T	C = 0.62	C = 0.61	0.8223	0.97	T
				T = 0.38	T = 0.39
707146	33064	150417864	C/T	C = 0.53	C = 0.48	0.1214	0.83	T
				T = 0.47	T = 0.52
707148	33958	150418758	C/A	C = 0.46	C = 0.48	0.6249	1.06	C
				A = 0.54	A = 0.52
707150	35182	150419982	A/T	A = 0.67	A = 0.67	0.9445	0.99	A
				T = 0.33	T = 0.33
5872184	38332	150423132	—/C	— = 0.00	— = 0.00
				C = 1.00	C = 1.00
3763015	40875	150425675	G/A	G = 0.61	G = 0.60	0.7976	0.97	A
				A = 0.39	A = 0.40
2042235	41624	150426424	T/C	T = 0.13	T = 0.20	0.0014	1.70	T
				C = 0.87	C = 0.80
3763013	41671	150426471	A/G	A = 0.97	A = 0.82	˜0.00001	0.14	G
				G = 0.03	G = 0.18
2042236	41825	150426625	G/A	G = 0.90	G = 0.86	0.0420	0.69	A
				A = 0.10	A = 0.14
1946234	42920	150427720	C/A	C = 0.00	C = 0.00
				A = 1.00	A = 1.00
1946235	42935	150427735	T/C	T =	T = 0.87
				C =	C = 0.13
1946236	43001	150427801	T/A	T = 0.19	T = 0.20	0.5482	1.10	T
				A = 0.81	A = 0.80
8177402	43012	150427812	T/C	T = 0.00	T = 0.00
				C = 1.00	C = 1.00
8177403	43203	150428003	C/T	C = 1.00	C = 1.00
				T = 0.00	T = 0.00
8177404	43294	150428094	T/C	T = 0.97	T = 0.94	0.0363	0.52	C
				C = 0.03	C = 0.06
8177405	43295	150428095	T/C	T = 0.00	T = 0.00
				C = 1.00	C = 1.00
8177406	43344	150428144	C/T	C = 0.09	C = 0.11	0.1222	1.36	C
				T = 0.91	T = 0.89
8177407	43509	150428309	T/C	T = 0.00	T = 0.00
				C = 1.00	C = 1.00
8177408	43549	150428349	G/C	G = 0.00	G = 0.00
				C = 1.00	C = 1.00
8177409	43560	150428360	T/A	T = 0.23	T = 0.28	0.0779	1.29	T
				A = 0.77	A = 0.72
6888961	43578	150428378	A/T	A = 0.08	A = 0.07	0.3176	0.79	T
				T = 0.92	T = 0.93
8177410	43640	150428440	A/G	A =	A = 0.00
				G =	G = 1.00
8177411	43792	150428592	G/C	G = 1.00	G = 1.00
				C = 0.00	C = 0.00
8177412	43797	150428597	C/T	C = 0.15	C = 0.18	0.0943	1.31	C
				T = 0.85	T = 0.82
8177413	43964	150428764	C/G	C =	C = 0.01
				G =	G = 0.99
870407	44297	150429097	C/T	C = 0.07	C = 0.11	0.0641	1.49	C
				T = 0.93	T = 0.89
870406	44311	150429111	C/T	C = 0.89	C = 0.88	0.5675	0.87	T
				T = 0.11	T = 0.12
6873202	44588	150429388	A/G	A = 1.00	A = 1.00
				G = 0.00	G = 0.00
8177414	44775	150429575	C/T	C = 1.00	C = 1.00
				T = 0.00	T = 0.00
8177415	44921	150429721	C/T	C = 1.00	C = 1.00
				T = 0.00	T = 0.00
3805435	45006	150429806	G/A	G = 0.10	G = 0.07	0.1318	0.71	A
				A = 0.90	A = 0.93
8177416	45098	150429898	T/C	T = 0.00	T = 0.00
				C = 1.00	C = 1.00
3792799	45185	150429985	C/G	C = 0.00	C = 0.00
				G = 1.00	G = 1.00
3792798	45475	150430275	T/C	T = 0.05	T = 0.10	0.0906	2.06	T
				C = 0.95	C = 0.90
3828599	45506	150430306	T/C	T = 0.55	T = 0.55	0.9993	1.00	T
				C = 0.45	C = 0.45
8177417	45543	150430343	G/C	G = 1.00	G = 1.00
				C = 0.00	C = 0.00
3792797	45601	150430401	T/G	T = 0.46	T = 0.52	0.0520	1.27	T
				G = 0.54	G = 0.48
8177418	45652	150430452	T/C	T = 0.00	T = 0.00
				C = 1.00	C = 1.00
8177419	45756	150430556	A/—	A = 0.00	A = 0.00
				— = 1.00	— = 1.00
8177420	45826	150430626	T/C	T = 1.00	T = 1.00
				C = 0.00	C = 0.00
8177421	45974	150430774	C/G	C = 0.07	C = 0.02
				G = 0.93	G = 0.98
4958872	46044	150430844	T/C	T = 0.81	T = 0.81	0.9167	0.98	T
				C = 0.19	C = 0.19
3792796	46200	150431000	C/G	C = 0.40	C = 0.41	0.5382	1.08	C
				G = 0.60	G = 0.59
8177422	46218	150431018	A/G	A = 1.00	A = 1.00
				G = 0.00	G = 0.00
8177423	46221	150431021	C/T	C = 1.00	C = 1.00
				T = 0.00	T = 0.00
4958434	46280	150431080	C/T	C = 0.86	C = 0.83	0.1864	0.79	T
				T = 0.14	T = 0.17
8177424	46330	150431138	—/	— = 1.00	— = 0.98
			GAGTCCTGG	GAGTCCTGG = 0.00	GAGTCCTGG = 0.02
8177425	46583	150431383	T/C	T = 0.02	T = 0.03	0.5080	1.30	T
				C = 0.98	C = 0.97
8177426	46650	150431450	A/G	A = 0.31	A = 0.36	0.0447	1.29	A
				G = 0.69	G = 0.64
8177427	46721	150431521	A/G	A = 0.26	A = 0.32	0.0296	1.33	A
				G = 0.74	G = 0.68
8177429	46808	150431608	G/C	G = 0.91	G = 0.95	0.0132	1.89	G
				C = 0.09	C = 0.05
6889737	47242	150432042	C/A	C = 1.00	C = 1.00
				A = 0.00	A = 0.00
3792795	47512	150432312	G/A	G = 0.89	G = 0.97	0.0000	3.53	G
				A = 0.11	A = 0.03
8177430	47600	150432400	T/C	T = 0.01	T = 0.01
				C = 0.99	C = 0.99
8177431	47706	150432506	A/G	A = 0.71	A = 0.70	0.6316	0.94	G
				G = 0.29	G = 0.30
4958873	47806	150432606	A/G	A = 0.31	A = 0.38	0.0279	1.32	A
				G = 0.69	G = 0.62
8177432	47978	150432778	T/G	T = 1.00	T = 1.00
				G = 0.00	G = 0.00
8177433	48021	150432821	T/C	T = 0.26	T = 0.23	0.2375	0.85	C
				C = 0.74	C = 0.77
8177434	48025	150432825	G/A	G = 0.00	G = 0.00
				A = 1.00	A = 1.00
8177435	48093	150432893	T/G	T = 0.81	T = 0.79	0.4469	0.89	G
				G = 0.19	G = 0.21
3763011	48413	150433213	T/C	T = 0.01	T = 0.02
				C = 0.99	C = 0.98
8177436	48933	150433733	C/T	C = 0.00	C = 0.00
				T = 1.00	T = 1.00
8177437	49097	150433897	C/G	C = 0.17	C = 0.07	˜0.00001	0.38	G
				G = 0.83	G = 0.93
4958874	49105	150433905	T/C	T = 0.44	T = 0.49	0.0880	1.24	T
				C = 0.56	C = 0.51
8177439	49570	150434370	G/A	G =	G = 1.00
				A =	A = 0.00
8177440	49591	150434391	C/T	C = 1.00	C = 1.00
				T = 0.00	T = 0.00
8177441	49704	150434504	G/C	G = 1.00	G = 1.00
				C = 0.00	C = 0.00
8177442	49705	150434505	A/T	A = 1.00	A = 1.00
				T = 0.00	T = 0.00
8177443	49798	150434598	C/T	C = 1.00	C = 1.00
				T = 0.00	T = 0.00
869975	50082	150434882	A/G	A = 0.37	A = 0.25	˜0.00001	0.57	G
				G = 0.63	G = 0.75
869976	50147	150434947	G/A	G = 0.00	G = 0.00
				A = 1.00	A = 1.00
8177444	50356	150435156	A/T	A = 1.00	A = 1.00
				T = 0.00	T = 0.00
8177445	50725	150435525	T/C	T = 1.00	T = 1.00
				C = 0.00	C = 0.00
7721469	50968	150435768	C/A	C = 0.00	C = 0.00
				A = 1.00	A = 1.00
8177446	51029	150435829	A/C	A = 1.00	A = 1.00
				C = 0.00	C = 0.00
7704191	51086	150435886	T/C	T = 1.00	T = 1.00
				C = 0.00	C = 0.00
8177447	51166	150435966	T/C	T = 0.27	T = 0.35	0.0030	1.50	T
				C = 0.73	C = 0.65
11548	51493	150436293	C/T	C = 0.90	C = 0.95	0.0002	2.40	C
				T = 0.10	T = 0.05
2230303	51539	150436339	G/T	G = 0.00	G = 0.01
				T = 1.00	T = 0.99
7722386	51562	150436362	G/A	G = 0.00	G = 0.00
				A = 1.00	A = 1.00
8177448	51645	150436445	G/A	G = 0.99	G = 0.99
				A = 0.01	A = 0.01
8177449	51649	150436449	T/C	T = 0.17	T = 0.14	0.2009	0.80	C
				C = 0.83	C = 0.86
2070593	51650	150436450	T/C	T = 0.31	T = 0.28	0.2540	0.86	C
				C = 0.69	C = 0.72
8177450	51656	150436456	G/A	G = 0.00	G = 0.00
				A = 1.00	A = 1.00
8177451	51657	150436457	T/C	T = 0.00	T = 0.00
				C = 1.00	C = 1.00
8177452	52009	150436809	G/A	G = 0.00	G = 0.00
				A = 1.00	A = 1.00
8177453	52143	150436943	A/C	A = 0.00	A = 0.00
				C = 1.00	C = 1.00
8177454	52349	150437149	G/C	G = 0.99	G = 0.98
				C = 0.01	C = 0.02
3763010	52421	150437221	C/T	C = 0.76	C = 0.80	0.1337	1.30	C
				T = 0.24	T = 0.20
8177455	52532	150437332	A/G	A = 0.00	A = 0.00
				G = 1.00	G = 1.00
8177456	52682	150437482	A/G	A = 0.45	A = 0.50	0.1098	1.22	A
				G = 0.55	G = 0.50
736775	53058	150437858	T/C	T = 0.56	T = 0.59	0.3945	1.12	T
				C = 0.44	C = 0.41
2277940	53187	150437987	T/C	T = 0.92	T = 0.97	0.0004	2.75	T
				C = 0.08	C = 0.03
8177458	53377	150438177	G/A	G = 0.98	G = 0.98	0.9445	1.03	G
				A = 0.02	A = 0.02
8177834	53699	150438499	G/A	G = 0.93	G = 0.89	0.0113	0.58	A
				A = 0.07	A = 0.11
3924	53845	150438645	A/G	A = 0.75	A = 0.74	0.7350	0.95	G
				G = 0.25	G = 0.26
2233312	53920	150438720	A/G	A = 0.00	A = 0.00
				G = 1.00	G = 1.00
2233311	53929	150438729	T/G	T = 0.15	T = 0.20	0.0106	1.49	T
				G = 0.85	G = 0.80
2233310	55473	150440273	T/C	T = 0.00	T = 0.00			T
				C = 1.00	C = 1.00
2233309	55690	150440490	A/G	A = 0.00	A = 0.00
				G = 1.00	G = 1.00
4958875	55850	150440650	A/G	A = 0.56	A = 0.53	0.2131	0.86	G
				G = 0.44	G = 0.47
2233308	56761	150441561	C/T	C = 1.00	C = 1.00
				T = 0.00	T = 0.00
2233307	56840	150441640	C/G	C = 0.00	C = 0.00
				G = 1.00	G = 1.00
2233306	57000	150441800	C/T	C = 1.00	C = 1.00
				T = 0.00	T = 0.00
2233305	57116	150441916	T/G	T =	T = 0.27
				G =	G = 0.73
2233304	58419	150443219	T/G	T = 0.00	T = 0.01
				G = 1.00	G = 0.99
2233303	58420	150443220	C/T	C = 1.00	C = 1.00
				T = 0.00	T = 0.00
2233302	58808	150443608	G/C	G = 0.98	G = 0.87	˜0.00001	0.14	C
				C = 0.02	C = 0.13
2287719	58906	150443706	A/G	A = 0.00	A = 0.00
				G = 1.00	G = 1.00
2287720	59048	150443848	C/T	C = 0.39	C = 0.42	0.2852	1.14	C
				T = 0.61	T = 0.58
7727034	59187	150443987	C/G	C = 0.24	C = 0.30	0.0374	1.36	C
				G = 0.76	G = 0.70
7727250	59361	150444161	C/T	C = 0.33	C = 0.43	0.0003	1.57	C
				T = 0.67	T = 0.57
7709800	61218	150446018	G/A	G = 1.00	G = 1.00
				A = 0.00	A = 0.00
3840312	61700	150446500	G/—	G = 0.54	G = 0.50	0.1346	0.84	—
				— = 0.46	— = 0.50
2287721	62290	150447090	G/A	G = 1.00	G = 1.00
				A = 0.00	A = 0.00
6875293	62596	150447396	T/C	T = 0.00	T = 0.00
				C = 1.00	C = 1.00
3805434	64049	150448849	G/C	G = 0.93	G = 0.87	0.0009	0.51	C
				C = 0.07	C = 0.13
2080982	66077	150450877	G/T	G = 0.02	G = 0.01
				T = 0.98	T = 0.99
2080983	66079	150450879	G/T	G = 1.00	G = 1.00
				T = 0.00	T = 0.00
2287722	66086	150450886	T/C	T = 0.00	T = 0.00
				C = 1.00	C = 1.00
2233301	66115	150450915	T/G	T = 0.00	T = 0.00
				G = 1.00	G = 1.00
2233300	66150	150450950	C/G	C = 0.00	C = 0.00
				G = 1.00	G = 1.00
4958876	66475	150451275	C/A	C =	C = 0.95
				A =	A = 0.05
2233299	69177	150453977	A/G	A = 0.48	A = 0.49	0.7917	1.03	A
				G = 0.52	G = 0.51
2233298	69210	150454010	A/G	A = 0.00	A = 0.00
				G = 1.00	G = 1.00
2287723	69312	150454112	T/G	T = 1.00	T = 1.00
				G = 0.00	G = 0.00
2161359	70244	150455044	A/G	A = 0.53	A = 0.51	0.5813	0.94	G
				G = 0.47	G = 0.49
7734456	70882	150455682	G/C	G = 0.53	G = 0.60	0.0188	1.32	G
				C = 0.47	C = 0.40
4292439	71905	150456705	T/C	T = 0.87	T = 0.84	0.1343	0.77	C
				C = 0.13	C = 0.16
4958878	72294	150457094	A/T	A =	A = 0.12
				T =	T = 0.88
6862024	72581	150457381	A/G	A = 0.80	A = 0.78	0.4512	0.89	G
				G = 0.20	G = 0.22
3834819	72786	150457589	—/CA	— = 0.00	— = 0.00
				CA = 1.00	CA = 1.00
2233297	72950	150457750	G/A	G = 0.04	G = 0.05	0.3215	1.32	G
				A = 0.96	A = 0.95
2233296	73106	150457906	G/A	G = 1.00	G = 1.00
				A = 0.00	A = 0.00
2233295	73162	150457962	T/C	T = 0.00	T = 0.00
				C = 1.00	C = 1.00
2233294	73273	150458073	G/T	G = 0.24	G = 0.23	0.7059	0.95	T
				T = 0.76	T = 0.77
7713028	74131	150458931	G/A	G = 0.9	G = 0.85	0.0238	0.66	A
				A = 0.1	A = 0.15
7713223	74406	150459206	T/C	T = 0.93	T = 0.88	0.0047	0.56	C
				C = 0.07	C = 0.12
7713567	74665	150459465	C/T	C = 0.61	C = 0.63	0.4135	1.10	C
				T = 0.39	T = 0.37
888989	74740	150459540	T/C	T = 0.90	T = 0.86	0.0352	0.67	C
				C = 0.10	C = 0.14
2233293	75382	150460182	A/G	A = 0.00	A = 0.00
				G = 1.00	G = 1.00
3749657	75400	150460200	G/T	G = 1.00	G = 1.00
				T = 0.00	T = 0.00
2233292	75460	150460260	G/A	G =	G = 1.00
				A =	A = 0.00
2112635	75863	150460663	C/T	C = 0.35	C = 0.36	0.7381	1.04	C
				T = 0.65	T = 0.64
871269	76098	150460898	T/C	T = 0.47	T = 0.42	0.0796	0.81	C
				C = 0.53	C = 0.58
3792794	78432	150463232	A/G	A = 0.21	A = 0.10	˜0.00001	0.41	G
				G = 0.79	G = 0.90
6579837	78604	150463404	G/T	G = 0.89	G = 0.91	0.1630	1.33	G
				T = 0.11	T = 0.09
3805433	79190	150463990	C/G	C = 0.28	C = 0.28	0.9484	0.99	C
				G = 0.72	G = 0.72
5872186	79870	150464670	—/A	— = 0.33	— = 0.35	0.5526	1.08	—
				A = 0.67	A = 0.65
2233291	79928	150464728	G/C	G = 1.00	G = 1.00
				C = 0.00	C = 0.00
2233290	80213	150465013	G/C	G = 0.14	G = 0.13	0.8704	0.97	C
				C = 0.86	C = 0.87
2233289	80227	150465027	C/T	C = 1.00	C = 0.99
				T = 0.00	T = 0.010
4958435	81994	150466794	T/G	T = 0.68	T = 0.63	0.0549	0.79	G
				G = 0.32	G = 0.37
4958880	82187	150466987	C/A	C = 0.75	C = 0.84	0.0002	1.76	C
				A = 0.25	A = 0.16
1422673	82698	150467498	T/C	T = 0.45	T = 0.32	˜0.00001	0.59	C
				C = 0.55	C = 0.68
2042234	82841	150467641	A/G	A = 0.91	A = 0.93	0.1485	1.37	A
				G = 0.09	G = 0.07
3805432	83214	150468014	A/G	A = 0.04	A = 0.07	0.0188	1.93	A
				G = 0.96	G = 0.93
3805431	83249	150468049	T/C	T = 0.06	T = 0.02
				C = 0.94	C = 0.98
2233288	83485	150468285	T/C	T = 0.00	T = 0.00
				C = 1.00	C = 1.00
2233287	83807	150468607	C/T	C = 0.90	C = 0.92	0.2555	1.32	C
				T = 0.10	T = 0.08
3815720	83907	150468707	G/A	G = 0.99	G = 0.97
				A = 0.01	A = 0.03
3792792	84216	150469016	A/G	A = 1.00	A = 0.99
				G = 0.00	G = 0.01
3792791	84656	150469456	A/G	A = 0.00	A = 0.00
				G = 1.00	G = 1.00
2303018	85448	150470248	G/A	G = 1.00	G = 1.00
				A = 0.00	A = 0.00
3792790	85881	150470681	G/T	G = 0.49	G = 0.49	0.9686	1.00	G
				T = 0.51	T = 0.51
4958436	86539	150471339	T/C	T = 0.88	T = 0.92	0.0083	1.76	T
				C = 0.12	C = 0.08
2233286	86796	150471596	T/C	T = 0.00	T = 0.00
				C = 1.00	C = 1.00
2233285	87057	150471857	G/A	G = 1.00	C = 1.00
				A = 0.00	A = 0.00
7732451	87922	150472722	T/C	T = 0.90	T = 0.92	0.2349	1.29	T
				C = 0.10	C = 0.08
2233284	88098	150472898	T/C	T = 0.00	T = 0.00
				C = 1.00	C = 1.00
1422674	89319	150474119	G/T	G =	G =
				T =	T =
3792789	89678	150474478	C/T	C = 0.42	C = 0.42	0.9873	1.00	C
				T = 0.58	T = 0.58
4562032	90026	150474826	C/A	C =	C = 1.00
				A =	A = 0.00
6865077	90033	150474833	G/A	G = 1.00	G = 1.00
				A = 0.00	A = 0.00
1559126	90114	150474914	C/G	C = 1.00	C = 1.00
				G = 0.00	G = 0.00
3792788	90326	150475126	T/C	T = 0.00	T = 0.00
				C = 1.00	C = 1.00
1559127	90463	150475263	T/C	T = 0.94	T = 0.94	0.6080	1.14	T
				C = 0.06	C = 0.06
3792786	90548	150475348	A/G	A = 0.98	A = 0.99
				G = 0.02	G = 0.01
6880110	90800	150475600	A/G	A = 0.91	A = 0.92	0.5433	1.14	A
				G = 0.09	G = 0.08
6861227	90838	150475638	G/T	G = 0.26	G = 0.23	0.1843	0.83	T
				T = 0.74	T = 0.77
3805430	91400	150476200	C/G	C = 0.02	C = 0.05
				G = 0.98	G = 0.95
1862364	92086	150476886	A/G	A = 0.91	A = 0.92	0.5575	1.13	A
				G = 0.09	G = 0.08
4958881	93946	150478746	T/C	T = 0.91	T = 0.91	0.9701	1.01	T
				C = 0.09	C = 0.09
3792785	95360	150480160	A/G	A = 0.92	A = 0.93	0.5448	1.16	A
				G = 0.08	G = 0.07
6869605	96576	150481376	A/C	A = 0.90	A = 0.89	0.7189	0.93	C
				C = 0.10	C = 0.11
6870205	96721	150481521	A/G	A = 0.00	A = 0.07
				G = 1.00	G = 0.93
4246047	98316	150483116	T/A	T = 0.94	T = 0.96	0.2613	1.37	T
				A = 0.06	A = 0.04
4958882	98497	150483297	C/G	C = 0.88	C = 0.89	0.7723	1.06	C
				G = 0.12	G = 0.11
3792784	99382	150484182	C/T	C = 0.00	C = 0.04
				T = 1.00	T = 0.96
3792783	99442	150484242	T/C	T = 0.85	T = 0.87	0.2391	1.22	T
				C = 0.15	C = 0.13
5872188	99764	150484565	—/AG	— = 0.90	— = 0.91	0.5908	1.11	—
				AG = 0.10	AG = 0.09

Allelotyping results were considered particularly significant with a calculated p-value of less than or equal to 0.05 for allelotype results. These values are indicated in bold. The allelotyping p-values were plotted in FIG. 4. The position of each SNP on the chromosome is presented on the x-axis. The y-axis gives the negative logarithm (base 10) of the p-value comparing the estimated allele in the case group to that of the control group. The minor allele frequency of the control group for each SNP designated by an X or other symbol on the graphs in FIG. 4 can be determined by consulting Table 27. For example, the left-most X on the left graph is at position 150385031. By proceeding down the Table from top to bottom and across the graphs from left to right the allele frequency associated with each symbol shown can be determined.

To aid the interpretation, multiple lines have been added to the graph. The broken horizontal lines are drawn at two common significance levels, 0.05 and 0.01. The vertical broken lines are drawn every 20 kb to assist in the interpretation of distances between SNPs. Two other lines are drawn to expose linear trends in the association of SNPs to the disease. The light gray line (or generally bottom-most curve) is a nonlinear smoother through the data points on the graph using a local polynomial regression method (W. S. Cleveland, E. Grosse and W. M. Shyu (1992) Local regression models. Chapter 8 of Statistical Models in S eds J. M. Chambers and T. J. Hastie, Wadsworth & Brooks/Cole.). The black line provides a local test for excess statistical significance to identify regions of association. This was created by use of a 10 kb sliding window with 1 kb step sizes. Within each window, a chi-square goodness of fit test was applied to compare the proportion of SNPs that were significant at a test wise level of 0.01, to the proportion that would be expected by chance alone (0.05 for the methods used here). Resulting p-values that were less than 104 were truncated at that value.

Finally, the exons and introns of the genes in the covered region are plotted below each graph at the appropriate chromosomal positions. The gene boundary is indicated by the broken horizontal line. The exon positions are shown as thick, unbroken bars. An arrow is place at the 3′ end of each gene to show the direction of transcription.

Example 10

In Vitro Production of Target Polypeptides

cDNA is cloned into a pIVEX 2.3-MCS vector (Roche Biochem) using a directional cloning method. A cDNA insert is prepared using PCR with forward and reverse primers having 5′ restriction site tags (in frame) and 5-6 additional nucleotides in addition to 3′ gene-specific portions, the latter of which is typically about twenty to about twenty-five base pairs in length. A Sal I restriction site is introduced by the forward primer and a Sma I restriction site is introduced by the reverse primer. The ends of PCR products are cut with the corresponding restriction enzymes (i.e., Sal I and Sma I) and the products are gel-purified. The pIVEX 2.3-MCS vector is linearized using the same restriction enzymes, and the fragment with the correct sized fragment is isolated by gel-purification. Purified PCR product is ligated into the linearized pIVEX 2.3-MCS vector and E. coli cells transformed for plasmid amplification. The newly constructed expression vector is verified by restriction mapping and used for protein production.

E. coli lysate is reconstituted with 0.25 ml of Reconstitution Buffer, the Reaction Mix is reconstituted with 0.8 ml of Reconstitution Buffer; the Feeding Mix is reconstituted with 10.5 ml of Reconstitution Buffer; and the Energy Mix is reconstituted with 0.6 ml of Reconstitution Buffer. 0.5 ml of the Energy Mix was added to the Feeding Mix to obtain the Feeding Solution. 0.75 ml of Reaction Mix, 50 μl of Energy Mix, and 10 μg of the template DNA is added to the E. coli lysate.

Using the reaction device (Roche Biochem), 1 ml of the Reaction Solution is loaded into the reaction compartment. The reaction device is turned upside-down and 10 ml of the Feeding Solution is loaded into the feeding compartment. All lids are closed and the reaction device is loaded into the RTS500 instrument. The instrument is run at 30° C. for 24 hours with a stir bar speed of 150 rpm. The pIVEX 2.3 MCS vector includes a nucleotide sequence that encodes six consecutive histidine amino acids on the C-terminal end of the target polypeptide for the purpose of protein purification. Target polypeptide is purified by contacting the contents of reaction device with resin modified with Ni²⁺ ions. Target polypeptide is eluted from the resin with a solution containing free Ni²⁺ ions.

Example 11

Cellular Production of Target Polypeptides

Nucleic acids are cloned into DNA plasmids having phage recombination cites and target polypeptides are expressed therefrom in a variety of host cells. Alpha phage genomic DNA contains short sequences known as attP sites, and E. coli genomic DNA contains unique, short sequences known as attB sites. These regions share homology, allowing for integration of phage DNA into E. coli via directional, site-specific recombination using the phage protein Int and the E. coli protein IHF. Integration produces two new aft sites, L and R, which flank the inserted prophage DNA. Phage excision from E. coli genomic DNA can also be accomplished using these two proteins with the addition of a second phage protein, Xis. DNA vectors have been produced where the integration/excision process is modified to allow for the directional integration or excision of a target DNA fragment into a backbone vector in a rapid in vitro reaction (Gateway™ Technology (Invitrogen, Inc.)).

A first step is to transfer the nucleic acid insert into a shuttle vector that contains attL sites surrounding the negative selection gene, ccdB (e.g. pENTER vector, Invitrogen, Inc.). This transfer process is accomplished by digesting the nucleic acid from a DNA vector used for sequencing, and to ligate it into the multicloning site of the shuttle vector, which will place it between the two attL sites while removing the negative selection gene ccdB. A second method is to amplify the nucleic acid by the polymerase chain reaction (PCR) with primers containing attB sites. The amplified fragment then is integrated into the shuttle vector using Int and IHF. A third method is to utilize a topoisomerase-mediated process, in which the nucleic acid is amplified via PCR using gene-specific primers with the 5′ upstream primer containing an additional CACC sequence (e.g., TOPO® expression kit (Invitrogen, Inc.)). In conjunction with Topoisomerase I, the PCR amplified fragment can be cloned into the shuttle vector via the attL sites in the correct orientation.

Once the nucleic acid is transferred into the shuttle vector, it can be cloned into an expression vector having attR sites. Several vectors containing attR sites for expression of target polypeptide as a native polypeptide, N-fusion polypeptide, and C-fusion polypeptides are commercially available (e.g., pDEST (Invitrogen, Inc.)), and any vector can be converted into an expression vector for receiving a nucleic acid from the shuttle vector by introducing an insert having an attR site flanked by an antibiotic resistant gene for selection using the standard methods described above. Transfer of the nucleic acid from the shuttle vector is accomplished by directional recombination using Int, IHF, and Xis (LR clonase). Then the desired sequence can be transferred to an expression vector by carrying out a one hour incubation at room temperature with Int, IHF, and Xis, a ten minute incubation at 37° C. with proteinase K, transforming bacteria and allowing expression for one hour, and then plating on selective media. Generally, 90% cloning efficiency is achieved by this method. Examples of expression vectors are pDEST 14 bacterial expression vector with att7 promoter, pDEST 15 bacterial expression vector with a T7 promoter and a N-terminal GST tag, pDEST 17 bacterial vector with a T7 promoter and a N-terminal polyhistidine affinity tag, and pDEST 12.2 mammalian expression vector with a CMV promoter and neo resistance gene. These expression vectors or others like them are transformed or transfected into cells for expression of the target polypeptide or polypeptide variants. These expression vectors are often transfected, for example, into murine-transformed a adipocyte cell line 3T3-L1, (ATCC), human embryonic kidney cell line 293, and rat cardiomyocyte cell line H9C2.

Representative Nucleotide and Amino Acid Sequences

Following is a genomic nucleotide sequence for a CTEP region. The genomic nucleotide sequence is set forth in SEQ ID NO: 1. The following nucleotide representations are used throughout: “A” or “a” is adenosine, adenine, or adenylic acid; “C” or “c” is cytidine, cytosine, or cytidylic acid; “G” or “g” is guanosine, guanine, or guanylic acid; “T” or “t” is thymidine, thymine, or thymidylic acid; and “I” or “i” is inosine, hypoxanthine, or inosinic acid. Exons are indicated in italicized lower case type, introns are depicted in normal text lower case type, and polymorphic sites are depicted in bold upper case type. SNPs are designated by the following convention: “R” represents A or G, “M” represents A or C; “W” represents A or T; “Y” represents C or T; “S” represents C or G; “K” represents G or T; “V” represents A, C or G; “H” represents A, C, or T; “D” represents A, G, or T; “B” represents C, G, or T; and “N” represents A, G, C, or T.

>16: 56742951-56841200
1     tgtctaaaaa ttattatggc tgaaacatac ttatcatttg
      ttttctgtag tagaaaatgg
61    cataatatga gaaggcctac tttgtaatag ttgtttgctt
      tacttaatat ttttcaaatc
121   agccatctgt gtgaagactc aatatcaagt taggccttta
      aaagggggaa aataattcaa
181   aaccactggc agctgtggtt ttccRgcctt ttctattggt
      tgaactctgt aataaatgca
241   ggaccctaca tatacaaaag gtactgtttt ccaaattcac
      gtatgtatta gcatttacct
301   ttaaagttct catccgtcag aaaaacggtg aacacagcat
      tcagtatatt tattttggtt
361   tagttacaca gtgtcaaaaa gttctgatgt atatggataa
      gtagatgcac tttttgctgt
421   tttgtttgtt taaacagctt gctttaatta tctcaagcta
      cttggcagga acacctttat
481   tccaggtttc gcataatcag ttattctggc aagagtagat
      taacacgtta gtgatctcca
541   atagacagat tatatgttaa tattatttga ggccattatt
      tttaagtaca atttagacgg
601   agtttcaaat agattttaaa aatgagattt gtataaaaga
      gtgtgcagtc cctatggtgt
661   taaaatcact gatatggagg agaagagggg ttggaattgt
      gacactgtgg cagcagatga
721   ccttggacaa gtcagttttt cagactttcc tcccttaaaa
      tagggatact aaatctcaca
781   gggtggttgt gtataatgtc acccacacgc catctcctct
      ataactgcat ttgtatgcca
841   ggaaattgtc tgtgaaggct tcagtttaga atagcttcat
      attattttgg ggcactgctg
901   ggtgattttg gttaaggctg gccttgggag ttctctgagt
      tttctctgaa accaaactca
961   atttggttgt aggttccctg tttgctgttt gttgctttta
      gtaataggag tcagtcagtt
1021  tacctgggag ttaagtttgg actggagata atttgccatt
      cttttttttt tttttttttt
1081  ttttttttga gatagggtct cactctgtcg cccaggctgg
      agtgcagtga catgatcttg
1141  gctcactgca acctctgcct ccctggttca agcgattccc
      ctgcctcagc ctcctgagta
1201  gtttgggtta caggcacaag ccaccacgcc cagctaattt
      ttgcattttt agtagagatg
1261  gggtttcacc atgttggcca ggctggtctc gaacccctga
      cctcaggtga tctgcccgcc
1321  ttggcctccc acagtgcttg gattacaggc atgagccacc
      atgcccagcc gataatttgc
1381  cattctgtat ttatttgcgt gttagagacc agttaaagtt
      aagacacttt ctaaatagaa
1441  gtcttgtata tggggctttt gagaaagtgt tttcccgtga
      tgctgggggg aaacacacat
1501  attcaaatta cttgtataaa acagtttatt ctgtgtttag
      gaacttgtag gggtgaaatg
1561  ctgacctgtg ttactctttc tttccatgat cagcRtccag
      aggaccagag gttaatttat
1621  tctgggaagc tgttgttgga tcaccaatgt ctcagggact
      tgcttccaaa ggtacatcac
1681  ttacacatta acttctgaat gtttttaagc actcaccagg
      ttaggttcag gtccttaagt
1741  accttcgtat tacttttagc aggaaaaacg gcatgttttg
      catctggtgt gcaatgtgaa
1801  gagtccttca aaaatgccag aaatcaacgc caaggtgtgt
      ctgcctcttc atgatggtaa
1861  caatttgtat cattcagact ttcaggggag taataaaaga
      agttggataa aacgtttatt
1921  gagagactgt atggtgttat taccaagtaa tattttgttt
      ctttcctagg aattagaaac
1981  tgtattatca acacaaagtg atgagttaat agtagcagat
      tgttccgtta gtgctgtagt
2041  agaaaggtgg agttacatat tgcactatct tatgggatat
      gatttgatct atggttactg
2101  tgtatctaga ttactgaact aacactaatc gggtttttat
      tgaatttagc acatagcaag
2161  tttcttcaaa ggagtcacta atttttataa agagtacaaa
      agtgaatata tttcctttgg
2221  aaattttcat tgtgattagt tggataggaa aagatcagag
      tttttatcaa gtgatcttta
2281  aagaattttt tttttttaaa aatggtctcg ctgtgtggcc
      caggcttttc tcaaactcct
2341  gagggcaagc gatcctccca cctcagcctc ctgagtagct
      gggactacag gcatgtgcca
2401  ctagacctgg ctctaaagac atatatgaca cacgaaacca
      tttatttttc atttcacaat
2461  gtttattcac atatatggta ttagtattct aatgtagtga
      tgcactctaa atttgcatta
2521  tatttcctag aacatctgaa cagagcatag gaaattccct
      attttgccat tatcagttct
2581  aacaaaaaac ttaaaagcac tttatcattt catttccctg
      cactgtaatt tttttaaatg
2641  atcaaaaacR gtatcatacc aaggcttact tatattggaa
      tactatttta gaaagttgtg
2701  ggctgggttg tatttataaa tcttgttggt cagatgtctg
      caatgagtaa atttagcacc
2761  attatcagga agctttctca ccaatgacaa cttcattgga
      agattttaat gaaagtgtag
2821  catactctag ggaaaaaata tgaatatttt agcatctatg
      tattgaaaat tatgttgaat
2881  aaatgtcaga ctatttttta cataacgttg cttctgttta
      attttgtcac gttcagaggt
2941  ggggggtagg agatgtaagc ccttgacagc aaaataattc
      cttttgcttg atttcagaca
3001  gttgcatcag ctcctttgtt ctgtgttcat gttacactta
      tttaggtggc tgaatccaca
3061  gaggagcctg ctggttctaa tcggggacag tatcctgagg
      attcctcaag tgatggttta
3121  aggcaaaggg aagttcttcg gaacctttct tcccctggat
      gggaaaacat ctcaaggtga
3181  gtgttataat aaagatcttg gcttatgcaa catgaatgtt
      cctcgtttgc atcaatttaa
3241  gaataaggta tgtttacacg tatataatca gaacttttaa
      acatacagaa ttttgcttta
3301  taaatagctt cgctttaaag atctcttata tatttaactt
      ttcttaatac acagcctttt
3361  agtacacaca aatttaaaaa gtaggtaatg catatattga
      aaaaaaaaag aaaatgtagg
3421  cgttttatcc ttccattgtg ctgaccgctt ggttgccgtc
      atgataggaa attagtgctg
3481  ctgcaggaga aaacagctgt cgtaagcatt gtgcagctgc
      tttgctgagt ggctttgtgc
3541  tttattgtaa caatgggtga caacaaggga gagactgttt
      aagaagtgtc cttccaaaga
3601  cttgggggta ctaggaaaat tggccaattt cttataacta
      ttaaagcttt tctttagagc
3661  aaaagtcaga actaaatgct ctgttatttg gatttttata
      gctcttggaa tacattgttt
3721  ttggggaaaa aftcattaac tgcaagttgc aattctgtaa
      ctctccaatt ctccgtcccc
3781  ctttttctag tactttttat acacaatatt ttataaagcc
      aggtgtttta ggaatgagtt
3841  tttcttcctt ttccccctta atggaacttt caaatataca
      caacagtggg gactggataa
3901  ttaatcccca caccatcacc caggcctaat aaataacaat
      cagagtttta ccatagctca
3961  tctattaccc tttccctttt ttttaaaaaa agagtattta
      aaagtaattc ccaggcatca
4021  tgttatttca tccctatatt cttcagtata taactatgaa
      aaccttttag ttatcttgta
4081  tatctagaat gccattatca gatctaacag aaatcttaaa
      agcactttat cattttattt
4141  ccatgcactg taattttctt aaatgatcaa aaacagcatc
      ataccaaagc ttacttgtat
4201  tggaatacta ttttagaaat actctgtgta tggaatttag
      ttaaaagatt gtcagcatat
4261  aaattggata attattggat agaaaattat tggttagaaa
      aacttaaaac tacttccctt
4321  attctgattc aactattctt aacttgagaa ttgaggctca
      tatttgactc tctgaatctc
4381  accatattta aacttgattt cctttaacaa atatttattg
      aacagccagt acatacaact
4441  ttgcttaagg atatgagttg acctgcagga atgaccacat
      aaccaatttc tgatctttgg
4501  gcaattctat tatggtttca attttgtgat gtgctactga
      agaaattgac tgtgtaagaa
4561  gcacaaagcc aaagatgact cgcatacacc tgccctgtga
      agttgagggc tgtgtggcct
4621  gattaaagaa gcatgcattt ggccgggcgc ggtggctcac
      acctgtaatc ccagcacttt
4681  gggaggccaa ggtgggcgga tcacgaggtc aggagattga
      gaccatcctg gctaacacgg
4741  tgaaaccccg tctgtactaa aagtacaaaa aattagccag
      gtgtggtggc gtgtgcccgt
4801  agtcccagct actggggagg ctgaggcagg agaatggtgt
      gaatccggga ggtggagctt
4861  gcagtgagcc gagattgcgc cattgcactc caggctgggc
      aacagagcga gactccatct
4921  caaaaaaaaa aaaaaagaag catgcatttg acatcagacc
      agctctgaaa tccagccctg
4981  ctacttacta gctcagtgac cttgtgcaaa gcccctgtct
      tccctgagct gtacttgggt
5041  tccccttgta aaatctcctt catgagattg ctgtggaccc
      agcagcctgt acaggacccc
5101  cggtggtcag taagcatgca caggtgatac cacatgcaca
      cttcactata gattcagatg
5161  gaggatggag gaagaagtgg atattgggag gatgggagag
      atttccttca ggaaagactg
5221  ggagaaatgt ggcttttgag ctgctctcta aaagatgaac
      agtgggttga gacctgcagt
5281  gagactcaac aagtcaaaac tgaggtggga tctacttaac
      ccaaaaaggt tgtttatata
5341  tctgtgcact cagcaagcag gtggcaaggg ctgttgtttt
      cctgtgggat gggactgtgg
5401  tagaagtgct gtgtcattta gtaccttctg tgcttgtggt
      acatgggtgc gtcatacagt
5461  catttaccat gtaactgccg taaattccat tcttcYgtct
      cttcgatttg aattcttgat
5521  tttaatttaa acttagtttt catttgttca tactcaaccc
      actcaaaatg agtgtttttt
5581  caaatctatg tataggcctg aagctgccca gcaggcattc
      caaggcctgg gtcctggttt
5641  ctccggttac acaccctatg ggtggcttca gctttcctgg
      ttccagcaga tatatgcacg
5701  acagtactac atgcaatagt gagtccttcc cgccatgctg
      ggtgtggcca gggctcccgg
5761  gaattgaagg gaattttatc cRtgttacct gtaaagttca
      gaatggaggg atgagtgtat
5821  tttcatcagt ctgcagagcc ctgctctgtt tggtctgtgg
      gtagagtgta aatgacagct
5881  gctacctgat ttgtgtattg acacaagRgt gcttttgtct
      gatacatagt ttagtgactt
5941  tatttagaac atgttacttt cttttcaatt tgtgctatct
      tcaaagtttt gtgaggattt
6001  taatttgcta ctgtgccata atcttacagt gggcttgatg
      tttaatttca ggtgaaattc
6061  accttgtggc ttttcttaga aaaggcattt atagatatag
      ttagtaagga agttcttcac
6121  taaattgaag aaaaatcaaa ggcatatcta cctacttgaa
      attcttaaca gtaaaagact
6181  gctgtgtaaa taagccacag acttcacggt gctttggtct
      caacagtatc tgcctctgtc
6241  gtttttattt tagtttagca gccactgctg catcaggggc
      ttttgttcca ccaccaagtg
6301  cacaagagat acctgtggtc tctgcacctg ctccagcccc
      tattcacaac cagtttccag
6361  ctgaaaacca gcctgccaat cagaatgctg ctcctcaagt
      ggttgttaat cctggagcca
6421  atcaaaattt gcggatgaat gcacaaggtg gccctattgt
      ggaagaagat gatgaaataa
6481  atcgagattg gttggattgg acctattcag cagctacatt
      ttctgttttt ctcagtatcc
6541  tctacttcta ctcctccctg agcagattcc tcatggtcat
      gggggccacc gttgttatgt
6601  acctgtaagc agatggtttc tctaatataa attacactac
      actgtgttca cactaagcag
6661  attttgctct ttttgtttct tgtttttttt gagatggagt
      ctcgctctgt cattcaggct
6721  ggagtacaat ggcacaatca ctgcaatctc cgcttcccag
      gttcaagcga ttctcctgcc
6781  tcagcctcct gggtagttgg gattacaggc gcccaccacc
      atgcctggct aatttttata
6841  tttctagtag agacggggtt tggtcatgtt ggccaggtgg
      tctcgaactc ctgacctcaa
6901  gtgatctgcc caccttggcc tcccaaagtg ctggtattac
      aggcgtgagc caccactgcg
6961  cctggccaga ttttgctctt ttttgagcag tctcagttac
      tgtagaagga gatgtgttta
7021  aatagtatat cactctgtgg ctgggcgcag tggctcacac
      ctgtaatccc agcagtttgg
7081  gaggccgagg caggaggatc acatgaggcc aggagtttga
      gaccatcctg gccaatatgg
7141  tgaaaccccg tctctactaa aaatacaaaa aattagccgg
      gtgtggtggc acgggcctgt
7201  aatttacttg ggtggctaag gcaggagaat tgcttgaact
      ggggaggcag aggttgcagt
7261  gagccaagat cgcaccactg cactccagcc tgggtgacag
      agcaagaccc tgtcttagaa
7321  taaatacata ataaatagta tgtattctgg cactttcgat
      acaaggaatt catggcttgg
7381  ttgtatggtc ccaagaacat atcaatcctg tgttaatata
      agaatattat cttgtcctct
7441  agataagcta ccttaccttc caggctcaca aaccacaagt
      atgtagccat actgaggcat
7501  ggaacagaaa actgtatttg ttttgaatga gaacacattt
      gcctttatca gRccacagct
7561  gccttccagg gtaaagtcag gcactgactg ttagcatggc
      cctgaaaggc aagggaacct
7621  tcacatacct gacatttggt tttagctgtg gcccgaagca
      gtagttctca actgggggtg
7681  gttttgtacc ctctctcccc aggggacatt tggcaatgtg
      tagatatttt tggttgtcac
7741  atctagggga agtggtcctg ctggcgtcta gttagtagaa
      gcaagagtgc tgctgaattt
7801  tctacaatgc acaggacaac tcccacagta aatatttggc
      tcaatatgtc actagtgcca
7861  aagctgagaa agcctggcct agagtgatgg gtcttcgtgg
      ttgaaactaa aagaagaaat
7921  tttcctgtat agtaaaaatg gatttttatt ttagctttta
      aaaataaaaa aggaataatt
7981  agaatataat gaacactcaa atatccacaa ttagtcattg
      ttaatatttt attatattta
8041  agacttggga tttaattggt ttcttcaaat aaaacgttta
      gttaatattt tctgtagtct
8101  ctagaatcat atatttaaaa gaccaaatgt aggttgtcat
      tatgttatat ataattatag
8161  ctttgtaggt atatgcattc tccccgctct gttcaggttt
      tcaagggtaa ggtttagttc
8221  gtcataagca tttattgagt gcatactatg tgccaggtgc
      tgtttatatg gctgggtgtg
8281  caatgatgaa taaaacacag tccctgcctt caaggacctt
      acagactggt gagaatgtct
8341  gggagacagt gtgatcaaat gcctctgtag atgggtcttg
      cagtgagctt atcagggtgc
8401  tgctgtgatt agaggtgggg agccttggat tcttgagtaa
      gtctcactgt gacatcagct
8461  aagatggact tttatgtgct tcctttgaag gcatcacgtt
      gggtggtttc catttagacc
8521  gaggccggtt cagaacttcc caaatgatgg tcctcctcct
      gacgttgtaa atcaggaccc
8581  caacaataac ttacaggtat ggagcctccc acgaagccca
      ggcgagcttg acgtgatatg
8641  ccaggctctc caatcctcaa cctttagatt gccagcttgc
      ggtttaccat tttattgggc
8701  taaatatttc atgatttgtt cacatttctt cttagagcag
      cagctatttt taaaacgtcg
8761  aatgtgccat cacattctat cacatatttt tgacgtggca
      atttgcattt tggcttaagt
8821  aaataacatt tttttaaacc cactattttg agcgttcagt
      ggtctgtaac agtgtgttat
8881  accataagaa ctggtatgaa gtggttaact actagtttaa
      taatagttga agcctgggcg
8941  tggtggctca cgcctgtaat cccagcgctt tgggaggctg
      aggcaggtag atcacctgag
9001  gtcaggagtt taagaccagc ctggccaaca tggtgacacc
      ccgtctctac taaaattaca
9061  aaaattagtt gggcgtgcct gtattctcag ctgctaggga
      ggctgaggcg gaaggatggc
9121  ttgaccttgg gaggtggagg ttgcagtaag ccaagatcac
      accactgcac tccagcctgg
9181  gtgacagagc Ragactctgt ctcaaaaata ataataatag
      ttgcagatct agttgtttca
9241  tttgatattt gctgccagga gcagtcaaaa ctatgacaac
      atcaacacag ttgtgctgtg
9301  gaagcctgag aaacagccct atccagccta gggcatcatt
      ccctggggtc ctggctgcct
9361  gctgctgtgc ctatggggca gggggcaggg atttaccctg
      ccctttccta acattatttg
9421  gtgttcatca tagccctaat tgttttctca ttgtttcatt
      acctcattgt ttcattacct
9481  gtaggaaggc actgatcctg aaactgaaga ccccaaccac
      ctccctccag acagggatgt
9541  actagatggc gagcagacca gcccctcctt tatgagcaca
      gcatggcttg tcttcaagac
9601  tttctttgcc tctcttcttc cagaaggccc cccagccatc
      gcaaactgat ggtgtttgtg
9661  ctgtagctgt tggaggcttt gacaggaatg gactggatca
      cctgactcca gctagattgc
9721  ctctcctgga catggcaatg atgagttttt aaaaaacagt
      gtggatgatg atatgctttt
9781  gtgagcaagc aaaagcagaa acgtgaagcc gtgatacaaa
      ttggtgaaca aaaaatgccc
9841  aaggcttctc atgtctttat tctgaagagc tttaatatat
      actctatgta gtttaataag
9901  cactgtacgt agaaggcctt aggtgttgca tgtctatgct
      tgaggaactt ttccaaatgt
9961  gtgtgtctgc atgtgtgttt gtacatagaa gtcatagatg
      cagaagtggt tctgctggta
10021 cgatttgatt cctgttggaa tgtttaaatt acactaagtg
      tactacttta tataatcaat
10081 gaaattgcta gacatgtttt agcaggactt ttctaggaaa
      gacttaYgta taattgcttt
10141 ttaaaatgca gtgctttact ttaaactaag gggaactttg
      cggaggtgaa aacctttgct
10201 gggttttctg ttcaataaag ttttactatg aatgaccctg
      gcagagactc ctgtcatcct
10261 agcagtttac tctgcgtttg ttgtatctag acagtcaaca
      actgagttgt cggtgtttaa
10321 cctgaatgct tgtttttcag aagaRgactg tttgtgccgg
      taagaatgat caggtaaggc
10381 catgaaagtt tttgttggyg tttttgtttt tgagatgggg
      tcttgctttg ttccttgggc
10441 cagagtacat tggctactca caagtgggct ggtagctggc
      tacagcccca gactcctggc
10501 ttaagccacc tcctgcctca gccaccctgg cagctgggac
      tacaggcatg cgccatcaca
10561 cttagcttga aagttttaat ttactaagaa tatacctgtg
      tttcccccca tttcctgatt
10621 taaacagtac tggcttatat aggaacccat caaagttaaa
      ttccccaaat ttaaatttag
10681 taaatttagt ggtttcacct tggcaaatct gcaatagttt
      caccagctca aatttcatgc
10741 ttttgtaagc tgagcttatg tttgtgattt taatccttta
      agtactactg tggtaactaa
10801 tcattttttt gttttttttg gtggtgtttt tgtttttgtt
      ttgagatgga gtctcactct
10861 gttgcccagg ctggagtgcc gtggcgcgat ctcggctcac
      tgcaacctct acctcctgag
10921 ttcaagcgat tctcctgcct cagcctcccg aatagctggg
      attacaggtg cccaccacaa
10981 tgtctggcta attttttgta tttttagtag agacgaggtt
      tcacccatgt tggccaggct
11041 ggtctcaaac ccctgacctc aggtgatctg cccgcctcgg
      cctcccaaag tgctgggatt
11101 acagatgtga gccaccgcac ccagccaggg actaatcttt
      aaagcaaagt tttatatatt
11161 tttacgtgag taatgttatg tgtaggtgtt ctatttggca
      aaataaatca gccttttcta
11221 tcatgattgt ggtcatttaa attaatcctc atcggaaaca
      ttgtttacct aagataaaca
11281 ttcactaaac aactcatagc aagacgctgg gtaggtacaa
      agatgtctta tgtgtggtta
11341 tgctttcaaa gccaactgaa acccttttgt aaggagttaa
      atagcaaaaa gtttcaaata
11401 actgtgtgcc tctagaacag aactatcaga aggcaatgaa
      tattcacaaa ttgtgaagag
11461 atagtttgct tcaaggaaga aatgacttgt aactagagta
      ggtatgaaat gatgggggaa
11521 aaatgatgag gtatgaaatg actcaccttc tctccagctc
      aaggtaagaa gggtggcagc
11581 aggagtaaaa gctcagccac aggacgtgct cttcctgcca
      aacagctgga ttctgcacat
11641 gcttgggaat gaggtcctgc tggtttagga aaacaaacat
      taagaattcc caaagtcttg
11701 ggtggaaaag cctgttgctt ttcagaggca aggccatcac
      catttggcaa ggggcccaag
11761 gccccaggca gctgtggtac catctgtttc tgaaggagtg
      gggattttac cccctgaaat
11821 gtcagtttgt ggcttaaact ctgggttcta caggcccaaa
      ataaatagtg ccaggaaggt
11881 gcccctgaac agagctaacc agggttcaga ttggcacccc
      atccccactg gggcaagcag
11941 gactcttagc aattaaggcc taagacggct gacaggtggg
      acatggcaga aattcagtgc
12001 ttccaccaca gctactcaat gtgccacRat catgggccac
      acaccactct gaatacttag
12061 aaaattattg gtttgggcct ttattattaa gtagcaagta
      aataactgaa aaagccccag
12121 atttcagctc tggctatagc agctttctaa gtctgtgttt
      ttaaggtgca atttcttgaa
12181 tcctagttct gtagaagaaa cactctaccc ccccgccccc
      cccacccccc ccccccaccc
12241 ctaaaaaatg agcttcatga tcaagtacat tttggaaccc
      tcaggctgat ccaagttcat
12301 agatttcttc actgcaggcc ttctgggtcc tttaatatgc
      ctgaagctgg ggctttccct
12361 tcctgagaat ggcccaggac tatccatgtg cctcattttg
      ggaaatttag taatgagtta
12421 ctaaatcaac accacctttg gcattctaca tagcaaacat
      actttgttag atgtgacatt
12481 ttacacacta accaatggag ataaaatttt ttaaatttat
      ttttggccag gcacggtggc
12541 tcatgcctgt aataccagca ctttgggagg ccgaggcagg
      cggatcactt gaggtcaaga
12601 gtttgagacc agcctggcca acgtggcgaa atcctttctc
      tactaaaaat acaaaaatta
12661 gctgggcgtc gtcgtgggca cctgtaatcc cagctactcg
      ggaggctgag ggaggagaat
12721 tgcttgaacc cgggaggggg aggttgcagt gagccaagac
      cacaccactg cactccagcc
12781 tgggcgtcag agtgagattc tgtctcaaaa aataaataaa
      tgatttctta tttatttttt
12841 tttaccagtg ctctacatgt tcagagaaac ttctctggta
      accaaccaca gaaatgatcc
12901 ctgaaagtat agtcttagga gataaatatt ctttacatag
      ccaggaggga gatgagaagc
12961 catacctgtc tacagtctga ctgacaaagg aggaggctcc
      aggtcccctg gagaatccca
13021 ggttttatgt aataattact tggtcctttt caaagtactt
      ggatgagtgt aggatagtct
13081 ctttgtctta tcagctagtc ctgcaatcta ggtgttctcc
      cattttgtag aggcttgcag
13141 agatgagaga cctgtccaag gtctcaaacc cctaactggc
      agttctgaga ctagaaatac
13201 tccctctcag gagtggacct cctctacaat tttttttagc
      agtcctcatg tgccaaatat
13261 ggcgtggggg ccaggagaca gaatgaggca taggttgggc
      gtggcccctc ccctcttggg
13321 tcttgtagtc ggctgtgggt ggcaagtaca gcagYgatgg
      cagacaggtg tgatgagttc
13381 aaggacaggg aaataacatc cacagcccca tcccagtact
      cctgtgcagc aatggaagtg
13441 tgccaccgac acaggggcta cagggtgagc aggagtgaca
      ggcgaagaga ggaagagagc
13501 tctgcctgtg caaaggcgga gggaacatgg tgtgggagga
      actgaagaga attccacata
13561 agtatgaaga gggtagggag attgagactg gagaaaaggg
      gaagccgggg ctcccctttt
13621 gaccatgcaa cgcactggaa gcacagcact cccagcctca
      gggtcctcca tgattggaga
13681 ctgacaRtga attcagagga gcttgtggaa gtcacccagt
      caggtccaga aatcctgcag
13741 tgcatccctt ccgttacccc tgtcataact gggagaagac
      accacccaca tgacgcaggg
13801 gagagtgcay gcatgagcta gcacccatat tgtcccctgc
      atctgtcctt ggctatgttt
13861 tacacccccc accccttaga acattgtagt caaactagga
      tgtattaaat tggctggttg
13921 ctcccaacta cactggacaa agtggagaaa aaaaaaaaag
      gaggagctca aggtttcaaa
13981 ttcccagctc aagctctgca taaaaactgc tgtgacttcc
      ttagaagaga cccctatctc
14041 ctgtagctgc agggttgaga tttctgagct caagtgatct
      gcctgcctca ccctcccaaa
14101 gtgctgggat tacagttgtg agccaccctg cctggccagg
      gctgagattt gtgaaaagga
14161 aacccaaagt ctcatcttgc atgtggctga attatgtcgc
      aaatccaatt cccaaattcc
14221 agccttgcag ggtgtcttca gttaaagtga ggacaacttg
      aaaatcggaa tgggaacata
14281 agggaagatt ccagtaaagc tggggacatt gaatccctaa
      attctgtYat gttccctgcc
14341 agtagaaaca gcccatctac ccctccctgg gtccagctgt
      gtggacacag attggcagcg
14401 agtgtgcagg tgttatttcc ctacccttgt aatcatcaca
      ctttcttttt ctttttcttt
14461 cttatttttt tgagtcagag tcttgtgctg ctgcccaggc
      tggagtacag tggtgcaatc
14521 tcagctcact gcaacctccg cctcctgggt tcaagcaatt
      ctcctgcctc agcctcccga
14581 gtagctggga ctacaggcgc gcaccaccac acccagctaa
      tttttttgta tttttagtag
14641 agacagggtt tcacccccaa tagccaggtt ggtctcgaac
      tcctgacctc aagagattca
14701 cccacctcgg cctcccaaag tgctgggatt acaggcgtaa
      gccacYgtgc ctgacctcat
14761 cacaatttct tagccctttg agaaaccctg tggccttccc
      tgaggtagct gtcttgcggg
14821 gcacttctga ttcttcttgg atcctatgcc cttcccctct
      ttgctggacc tgtaactaga
14881 ctcaagtccc accaggcccc agagagaaag gtacacactg
      tgacccatga agaggtgtgt
14941 tacacaccaa aagaactgca tgatttttcc aatttataca
      gacaaatcca agagaYatgt
15001 atgggagtag attttaaggt tgtgagatca cggaaggacc
      atagtgttgg atctggctga
15061 acgtaatgat atgggcccac taagcagaga ttcttaactc
      agtgctttag catgaggggt
15121 tagaaagggc tctaacttgg ttggttggtt gtttggttcg
      ttggttggtt ggctggctga
15181 aacatggacc aaaaggtagg tggccgacac cgttccttat
      tttgttatta atatatttgt
15241 atattcatat acaaacttgt atgctatgat atctaataca
      tattacagat ataaaacaaa
15301 tctttaggcc aggcatggtg gctcactcct gtaatcccag
      cacttcggga ggctgaagca
15361 ggtggatcac gaggtcagga gttcaagacc agcctggcca
      acagagtgaa atcccatatc
15421 tactaaaaat acaaaaatta gccggtcacg gtgtcaggca
      cctgtaatcc cagctactca
15481 agaggctgag gcaggagaat tgcttgaacc cggaaggtgg
      aggttgcagt gagctgagac
15541 cccatcactg cactccagcc tgggtgacag agcaagactc
      cgtctcaaaa aaaaaaaaaa
15601 tcttttgttt ctttcctctc tcattccttt atcacctaac
      ataagatgga tcatcacagc
15661 attgaagtga caggatgtca agcagaagag taaacatcac
      ccaaggacgt tgcagcctct
15721 ctggggaaag gattggcatg ttttctgttg tatgcaggat
      ggttgagtca tgttaggtgg
15781 aagtatggct ttgttactgt ctttatctgg agattaagtg
      tggtttaagg agatgcatac
15841 aggtgccagg ttgacaaaga gagtactgta atgattagtt
      ttatgtgtga acttggctag
15901 gcccagttat gtaaacacga atctaggccg ggctttggtg
      gctcacgcct gtaatcccag
15961 caatttggga ggctgaggca ggcggatcac ctgaggtcag
      gagtttgaga ccagcctggc
16021 aaacatggtg aaaccccatc tctaccaaaa atacaaaaat
      tagcctggcg tggtggtgtg
16081 tgcctgtagt ctcagctact cgggaggctg aggcaggaga
      atcgcttgaa cctgggaggt
16141 ggatgctgca gtgagtggag atcatgccac tgcactccag
      cctgggcaac agagtgagac
16201 tgtatcgcaa aaaaacataa aagaaaaaac aacaaaaacc
      ccgtgaatct aggtgttact
16261 atgaaggtat tttgcagatg tgattaacct gcatgggcct
      catctaatta tctgaaagac
16321 cttaattgca aaactcaggg ttccccaagg acagagcaat
      taagtctatg gcctgcagcc
16381 tcagcttccg cctgagtttc cagcccacca tcctacccta
      cagcttcaga cgcgtctagc
16441 cagcccctac aactgtgtag gacaatttct agaagttagt
      taatgaatac gctattggat
16501 ctgtttctct ggaggaaccc tgactgacac aggggccggc
      tctggaggtt gtggggtcca
16561 gttccttatc cctgtgtagt gtattggcca gttggggtgg
      gaattgcttc caggcaggct
16621 gtgtcatttg ttcaggcagg gtcccctccc cttggctctg
      cctgagagca aaggcaggtg
16681 ggggtggccc tggggagggc ctgaagcttt gctctgtgcc
      tcctaaagct ttcccactcc
16741 agggaccagg ccaggcaggc tggcaggaag aggcaggctt
      catctggaag gatgtctcca
16801 gggtggaaat gatgagattt aaatggtgta gggacccgga
      gggtggggtg ggacagcacc
16861 actgacccag ctgcagagtt ggaccctctg aagcccattg
      tgtacatgag aatctgtgtg
16921 cctgattgcg taggtgccta tacatggata ggctgcgaat
      ctgtgtgcac acagctggct
16981 ggagagcaca tgagtaggta tatgtaccac attgtcagca
      ctcacccatt agctcctggg
17041 gactcacttg ctgcaggtga tccctgtcct cgtgggctct
      acaccctggc aggtcaacac
17101 agactacaaa gaaacaagcg gacataaaat gacaagggct
      aggacaaaaa gcatggaaag
17161 gagaggggat aacgtggcag gggacatttt agaatgcagt
      ggtttcgaca ccaggaggag
17221 gagtggttag acagtggtcg gggagggcct ctgaagaggt
      gacacctgaa tgatgagatg
17281 gagcaaacca agcaaagatt ggaggaagag cctccaagca
      gaaagaactg caaggacaaa
17341 ggccctgggg caggagcggg tacgtgtgtc ctgtatgtgt
      gtgtcctgca cggtggcagg
17401 tgtctgaagc tgagcccaga ggagctgtgg agatcaggcc
      cagaagtgga gccacagcac
17461 gtagggccag agacctgcag taagaaatgg gatgtggtcg
      agatgggatg ggagccaccg
17521 cagcaccatg gggcgggagc agcagagtct gatgttgtgt
      acttcaggga gctggagttc
17581 tatgaaggaa gagcgaggag gcatgtggga ggaagaacag
      ccccactgag gcctgcaggg
17641 aaggcagcag accccaggtc agagcatgaa ggtagagggg
      aggttccaag aagtgggttg
17701 gggaataagc actggatggc tgatgacaga gggcagggtg
      gaggatgcgg ggccagggag
17761 ggctgcacag agtcctggga gggtgagcgg tggccctggc
      tggctaggag gcgccacctt
17821 gaggtgctac tggaggatac gccgcaggga aggtggatgg
      tctcttctga tgacaacctt
17881 cttgctggca ttagactgaa aggggaggtg atttgtaggg
      atgaggccag aggggttgtc
17941 cccagggaag ggcaccagat gtggggcagg agagctggtg
      tgagttgcag ccgtggggcc
18001 tagaggaagt cacagcaccc cactgaattc tcacttcctc
      atcataacag accctgtgac
18061 atataaaacc tgctctgccc accgcacagg gatcacaagg
      ctggagtgaa ataatggagc
18121 acttccccag agtggcacac gggacccagg gacgggtgtg
      caaaaccgtt ctaggtggaa
18181 gaacactttt tttttctttt tctttttctt tcttttcctt
      tttttttttt ttttttttga
18241 gatggagtct ccgtctgttg cccaggctgg agtgcagtgg
      tgcaatcttg gcccactgta
18301 gtccctggct cccaggttca agtaattctc ctgcctcagc
      cttctgagta gctgggacta
18361 caggcaccca ccacaacatc tagctaattt ttgtattttt
      agtagagaca gggttttacc
18421 atgttgacca gactggtctt gaactcctga cgtcaaggga
      tccatctgcc ttggcctacc
18481 aaagtagctg ggattacaag catgagccac cgtgcaaagc
      caaaaacact tgattataat
18541 agcatgctat ttttcaatgt attagataaa tgtattttcc
      atttaaagtg gggatataaa
18601 gctccccttt aaaatccaca taagtaaaaa agtcagtctg
      tttaaaaggt taagtaaata
18661 acagtctagg gcagatgcac aaaagacagc atcatgaccg
      tggttcctga aggattaagg
18721 ttgcaggaaa cactgaatgc taaaagcttt gtgaatgtat
      agtccatgaa aatgtctttt
18781 aaaaaatgag gtgcatacca agatgtttga gtgtgtgtat
      ttggggtagg tgctgtgggc
18841 atacgggtgt acctgtgtgt gtctcacaag caggtgtagc
      tgggtgcttg tgtgtatgtg
18901 gtgtgtgtgt atctgtgcac acgtgcatat ctgtgcccac
      atatccatgc acacgtcctc
18961 ataaggctct ctgcggccag gtgcggtggc tcacgcctgt
      aatcccaaca ctttgggagg
19021 ccgaggcggg gggatcacga agtctgaagt tcgagaccag
      cctggccaag atggtgaaac
19081 cccatctcta ctgaaaatac aaaaaattaa gacagtgagt
      gttgatcgtg aagaagggaa
19141 gatgaatgtg gggattaggt gggaggaatg gggtgctgtc
      aagatgaaaa taaacttagg
19201 gtggagaggc tggggcaaaa gagaggccca acagtatgat
      ttgccccagt gcggggactt
19261 tgtgcctggt gatttgaggt ggcagggctt ctacccaggc
      ctgatgggag ctgggattaa
19321 aagcagctgt gacctaaggc aggccattgc tctgtggcct
      Rctcccctaa ctcttttgca
19381 gaaagtccca ggctaagaga tgggggatgg ttgtaatgac
      ccctcccaga aatcctggct
19441 ctgtcctgca gaggtggggc ccggggatca agacgcctct
      aggttcaaga gagaggcaga
19501 atgaggggtc agccagctgg ggcaggaagg cttcactcta
      ggctgcaagg agagaccttt
19561 cgccttgact ggaaggacgt gaggagcctg tggcctcctg
      tttgcaacca agggctgagg
19621 aaggggccgg gcattcctgc tgtaggtcaa tgacctggag
      aaacacatgc ttgaggacag
19681 agctcagatc attgtcccag cgcaaacctt ggtgggatgt
      aaggtttgga gcttcttaag
19741 ttccaagagg cagtctcatg cagttcaagg agggctggag
      aagcctggtc tgctcatggg
19801 agtcaattgt taaattttca ggaattttgt gagccaatta
      ttgaacacag ctattattaa
19861 aaacaaaatt ataggctggg cgtgatggct catgtctgta
      atcccagcac tttgggaggc
19921 caaggcggtg gatcacttga ggtcaggagt tcaagaccag
      cctagccaac atagcaaaat
19981 cctgtctcta ctaaaaatac aaaaacatta gtcgggtgtg
      gtggctcaca tctgtaactc
20041 cagctacttg tgaggctgag gcatgagaat tgcttgagcc
      caggaggcag aggttgcaat
20101 gggctgagac cacgccattg cactccagcc tgggcaacag
      agggagattg tgtctttcaa
20161 aataataaaa taaaataaaa taaaataata ggctgggtgc
      cgtgactcac gcttgtaatc
20221 tcagcacttt gggagaccaa ggctggaaga tcgcttgagc
      ccagaagttg gagatcagcc
20281 tggggaacac agggagaccc tggtctgtac aaaaaataaa
      aacaaaatta gccaggcatg
20341 gtggcacatc cctgtggtcc cagttactta ggaggctgag
      gtgaacaaag ttaacactca
20401 aaactcatca cttgtgaatt actttgctac agtgcactat
      tattattatt tatttatttt
20461 tactgggcag cctcttgagc ctaagtaggc ccagagactg
      tccagcatac tattattgat
20521 gctttgtggt tattgatatt gattggatcc gtatagtgga
      aatggctata ctgtagaatt
20581 gtgtgctact gtgtatttct taccaattct gcattcagtg
      gcatcacatt catagcttga
20641 agttggccat ggtgggaggt aaatatgtgt ggtataaatt
      ggcaaagact acagattggg
20701 gttcctgtct ggccccccaa gagctggttg ttaaactttt
      accagcacac cactgagttg
20761 gagtctggaa agcaggcttc gtccaggttc tgccctgcta
      gctcaactgg ctctcaaagc
20821 tttgctccac caactggcct atggctgctt ccactcacca
      attctccctt ttgccctcta
20881 aggccacctc ttccaggaag tttcccctga tttctttgcc
      tggacttcta aaattctttc
20941 tttgccaggc gtggtggctc acacctgtaa tcccagcact
      ttgggaggac gaggtgggca
21001 gatcacttga ggtcaggaga tagagaccag cctggccaac
      acggcgaaac cccgtctcta
21061 ctaaaaatac aaagattagc tggacatggt gacgcgcacc
      tgtaatccca gctactcagg
21121 tggctgaggc aggagaatca cttgaacccg ggaggcagag
      gttgcagtga gccaagatca
21181 caccattgca ctccagcctg ggcaacagag ggagactcca
      tctcaaaata aataaataat
21241 taattctttc ttacctcttc cgttcatatt catacagaat
      aatctccaca tctcgaaatt
21301 cttaactgat tagctagttc ttgctattaa ttaaatacgt
      gatgcatatg gccaagcttg
21361 gatggatgga atccctgtat tggtaaggtg ggaatttttc
      ttttcttttt tttttttttg
21421 agaccaagtc tcactctgtc acccatgctg gagtgcaatg
      gtgtgatctt ggctcaccac
21481 aacctctgcc tcccaggttc aagccattct cctgcctcag
      ccttcctagt agctgggatt
21541 acaggcacac accaccaagc ccagctaatt tttgtatttt
      tagtagtgac agggttttgc
21601 catgttcgcc aggctggtct ggaactcctg gcctcaagtg
      atctgcccac ctcagcctcc
21661 caaagtgctg ggatcacagg tgtgagccac cacgcccacc
      tagggtggga attttccacc
21721 tgggttctgg gctcagctct gccacagatt cctctctctg
      ggcttatcct ctgaRggcga
21781 gattttggca tgactgagtg gatggaggaa gaacttgggg
      tcagcgggct gtcaccctgc
21841 tctgccgtgg aaccgactca acaacagggc cacaacaggc
      cttgttggac atggaagcaa
21901 atgagaaaat tagtaatact gattcagtgt ttacttaagg
      ttctgatact ttgtccatta
21961 tagacctttt gcattaattt tgatttttta ttatataaaa
      tatatatata acataaaatt
22021 gatcatttta gccattcgaa gtgtacagtt ttgtggcgtt
      taatacattc acattattat
22081 acaacccatc tccagaactc ttttcacctt gaaaaactga
      aactctattt ccattaaata
22141 tgaactcact ccccgttctc tcttcctcca gcctctggtg
      acctccattc tattattatt
22201 attattatta ttattattag agagagtgtc cctctgttgt
      ccatgctgga gtgcagtgat
22261 gcaatcacag ctcactgtag cctcaaactc ctgggctcaa
      gggatcttcc cacttcagcc
22321 tcctgagtag ctgagattat aggcatacgc caccacactc
      tgctaattca cttatttttt
22381 ggaaagatgg ggtcttgctg catggtccaa gccagactta
      cattcctggg ctcaagcgat
22441 cctcccacct caacctccca aaacattgag attacaggtc
      tgagccaccg caccctgccc
22501 attctacttc ctggctctta aatatgacta ctctagatac
      ctcatataag tggaatcata
22561 cagtatttgt ctttgtaact ggttcatttt acttggcata
      atgtcctgaa ggttcatcct
22621 catcgtagtg tgtgtcagaa tgcccttcct tttaaaggat
      gaataatatt cccttgtgtg
22681 gatatatcac atgttgttta tccactcatc attgatggat
      gcctgtgttg cttccacttt
22741 tggctgctat ggaaaatgct gctatgaata cgggtgtgca
      aatattcatt tgagtccata
22801 attttgattg ttttttcttt ttgagacaga gtctcacact
      gctgtccagg ctacagttca
22861 gtggcgcaat cttggctcat tgcaacctcc acttcccggg
      ttgaagtcat tctcctgcct
22921 tagcctcccg agtagctggg attacaggta tccgccatca
      cacctggcca atttttgtat
22981 ttttagtaga gacagggttt caccatgttg gagactggtc
      tcaaattcct ggcctcaaat
23041 gatccaccca cctcggtctc ccaaagtgct gggattacag
      tgagccacca tgccgagtcc
23101 ataattttga tttttaaatg gtggctacta agtttttctg
      gcacccctta aattttgtca
23161 ggcacttgcc ttaccctagt cctggcccca gtttgggttg
      tcacagcaag gtggaagtgt
23221 actgaaaagc tcgtgtttta tctttatacR tttacatggg
      gccccaaaaa gggggaaaac
23281 ccactttgat agggatatga cacagggacg caggagccaa
      ccgaaagagc ccccagtggc
23341 caaagatsga gcaacttgag caacaaaata aataaagtaa
      gcatcaaatt atagcccaaa
23401 gtataaaata agtgtctatg agtctatact cacatagatg
      atttaataca ttcatattaa
23461 taaatgggag agcactttga gaggccaagg tgggcagatc
      acttgagccc aggagtctga
23521 gaccagatta ggcactgtga tgaaaccctg tctctaccaa
      aaacacaaaa attagctggg
23581 catggtgggg catgcctata gttccagtta cttgggaggc
      tggggaggga ggattgcttg
23641 agcctgggag acggaggttg cagtgagctg tgatctcacc
      actgcactcc agcctgggca
23701 acagagtgag gccctgtctc aaaataaata aatatataaa
      taagataaat atataaataa
23761 aaataaaaat aaacgggaaa gaagagacaa atcttctgtg
      caggagaatY ccaaatgaat
23821 tctgtagatt ctccacccta caggagggca cacacaactc
      caggctgcct tagtgacctt
23881 cttccaaaga gtacagtacg ggaaggggga gcggggagaa
      ttaacttcac agtggagaaa
23941 tctgacaaat acgacctccg ccaagtgatc gaggtcacat
      cagctgtcat gaattatgtt
24001 gatagtgggc atcctggata tgatgggatg aaatggcact
      ttacctccac gacccttttc
24061 acaacagccc gtaatgccag tataaggaaa acatcagacc
      aggagtggtg gctcacgctt
24121 gtaatccgag cactttggga ggccaaggtg ggtggatcac
      aggtcaggag tttgagacca
24181 gcctggacaa catggtgaaa tcctgtctct actaaaaata
      cacaaactag ccaggcacgg
24241 tggcgggtgc ctgtaatccc agctacttgg gaggctgagg
      cagaagaacc gcttgaaccc
24301 gggaggcgga ggttgcagtg agctgagatc gcgccactgc
      actccagcca ggttgacaga
24361 gtgagactcc atctcaaaaa aaaaaaaaaa aaaagaaaag
      aaaagaaaaa gagaaaagaa
24421 aaacatcaga cagatcccaa cagaagggca tcctacagta
      tacRtgacca ctgctcctca
24481 aacctgtcaa gactatcaga aacaagagaa actgtcacag
      ctacaaggag atgtgacaat
24541 taattgtgat tttttttttt gagatgtggt ctcgctttgt
      cacccaggct gcagtacagt
24601 ggcacaatca cagctcactg cagcctcgaa ctctggggct
      caagtgatcc tcccatccat
24661 ctcagcctcc caagtagctg gggctatatg cgtgcaccac
      cacacccagc taatttttgt
24721 atttttttag agacggtatt tcgccatgtt gcccaggctg
      gtctcgaact cctggattca
24781 agcaatccac ctaacttggc ctcccaaagt gctgggatta
      caggcatgag ccaccatgcc
24841 ttgccaaatg tgatgtattc ttgatgggat cctggaagag
      gaaaaagata ttaggtaaaa
24901 actaaggaca tctgaataac catggatttc agtaatgtat
      ccacactgat tcattaattg
24961 taacaaatat accgtatgaa tgcaagatgt tcataatgag
      gccggggcgg tggctcatgc
25021 ctgtaattcc agcactttgg gaggctgagg cggaaggatc
      tcttgagctc aggagttcga
25081 gaccagcctg ggtaacacag tgagatcccc atctctacaa
      aaaatttttt gaaattagcc
25141 aggtgtggtg gtggcacatg cctgtagccc cagctactca
      gaagactgag acaggaggat
25201 tgcttgagcc gggaggttga ggctgcagta agccaggatc
      acgccactac actgcagtct
25261 gagcaacaga gcgacaccct gtctcaatga taataataac
      aataataata ataatggaaa
25321 ctcagtatgg gttgtatatg ggaaacttgc tcagtttctc
      tgaaaatcta aaattcttct
25381 gaaaatcaaa gtctgcttaa aaattcacat ggggtttgca
      ttctcctcca tgacaaggtt
25441 ttgcaggtta tgattacaga ggctggagag ggtgcaggtt
      atcccgcccc tccctgtccc
25501 agggattccc ttccccagga gctgtgtctc ccctgtgaga
      gagggtgagc ttccatgacc
25561 ccaagcctct tgccctctga ctccggtatt cttagaagct
      gggaccagca ctgagcccaa
25621 attcccgaag cgctcaaata ctggctttct gtccctatgt
      gacctggagc ttgtagttta
25681 acttctctct gcctcactgt ttgacctata aagcagggca
      atcaaggcat cccgggggtg
25741 gctatgaaga gtgaatgaga tagcagacaa cccagatgcc
      taccgacagg tgaagggacc
25801 aacacagtgc ggtataggcg tataagggaa tggagtatgg
      acacagccta caacacagac
25861 aaaccttgaa gacattcctc taagggacat aggccaggca
      tggtggctca cacctgtaat
25921 cctagcattc tgggaggccg aggcgggcag atcacttgag
      gtcaggagtt gagaccagcc
25981 tggccaacat ggcaaaatcc cgcctctact aaaaatacaa
      acattagctg ggtgtggtgg
26041 taggtacctg taatcctgga tactcgggag actgaggcaa
      gagagttgct ggaacccggg
26101 aggtggaggt tgcagtgagc cgagattgtg ccactgcact
      tcagcctggg cgacagagcg
26161 agactctgtc gaaagaaaga aagggaggaa ggaaggaaga
      aagaaaggga agggaaggga
26221 agggagggga ggggagggga ggggagggaa gggagagaga
      aagaagaaag agaaagaaag
26281 gaagaaagaa aaagaaggaa agaaagaaag aaagaaagaa
      agaaagaaag aaagaaagaa
26341 agaaagaaag aaagaaagaa agaaaagagt gaacccagga
      acgaaagatc acacacactg
26401 tatgactcta tttacatgaa atgttcagag taggcaaatc
      catagagaca gaaagcacat
26461 ttatggttgc caggagctgg gaaagggcag gatggggaat
      gactgtttat tggatgtggg
26521 gctctatttt ggggtgatga gaatgttctg gaattaaatt
      catggctgca taacactgtg
26581 aacatactaa atgcccctga attgtacact ttaaaatggt
      taaagtggca agttttcact
26641 aagcagtaaa ttaaattcta ctacaatttt aaaaagacta
      aaaaataatt taaaaaagat
26701 taaatgagat aacgcaaaaa agcattatct cgaaaataca
      gctgatatta gtataattct
26761 tactaagttt taagagtcta aggtgcagga ttctaagttt
      aaagggatag gctcttttgg
26821 ttttttggtt tagttatttg gttttttttt ttaatccatt
      atccccaccc ttgggaggcc
26881 cccagcaccc agtctgcact agaggatggg gcccacctcc
      cttttctctc caggcccagc
26941 cactgaccac cagtaccctg gccaggggca ccctcggtca
      ttgccctccg tggcccaagg
27001 aagggaacag aaacaacagc caagaagaca atagccgccg
      ggaagtcctc acatttctgg
27061 agaaatagag cccattaatg aatgaagttc ctccagcctg
      atcggaggac ggggtgctgg
27121 ggaggcctgg gctaaagggc tcacctccag cccccaccct
      ggcagggccg atggtacatg
27181 ctcactcagt gagggggctc cagaggtctg tgggtacgaa
      cccaagggct ggtgcccagg
27241 ggcaatcagc ttatgtctct gagccttggg aaacagtgag
      ggtcagcccg gctccccacg
27301 tgcttctggg cagctttggt attggagcag gtgcaaactc
      Rggactaggg caggaccccc
27361 tgagaggcga ctgagcaagg ccatcccgac tcatgtttcc
      ttggccctgc ccggggcaca
27421 gcatcctgcc cacatccctg cagccctggc tccttcctag
      gggctctgag gaggcagcac
27481 ttggtcatct ggtcacagtt gctgcagggc agttcttggc
      cccagctgta ggtaaagtac
27541 tgtatgttgt aattttttga aagataacac gttcacacaa
      ctcagaattg aaatgccaca
27601 gacattcccc ctgctccgcc cctttccccc ggatacccag
      tttctcccgg aggcagccaa
27661 tgatctcaga ggctgtatac ccccccagag ttattttatg
      catatcaagg aaagtctaca
27721 tagaggactg tttctggggt acccagatgc agcgtcaaat
      gccatggaat actacagtga
27781 ggacattatc ctttcaagct ttcaaatcag agcaagggaa
      aggtcgatgc tagagtttct
27841 ctagcaccca tgaagccctc tccctttttc tactgagttt
      tactttacag gcaacagcag
27901 gcttcaagct tggggtcatt gtcgggcaac agtatctggc
      aagaattcaa tgtctttttc
27961 tcatagtcat tgtattttgg cctctttcta tttatggcaa
      ctgagagaga aagcttattc
28021 ctagatatat gtatttaagt aaaaaataaa tgaattcatg
      gaaacatatt aagcaattat
28081 ccagataaca taagggatgg caaaaatggt gcagatggtg
      gaggggagac aagtagaagt
28141 tggggtgctc ttgttgaatg tctggctctg aactctagag
      gaggccgcag gggctgggca
28201 ggaaggaggt gaatctctgg ggccaggaag accctgctgc
      ccggaagagc ctcatgttcc
28261 gtgggggctg ggcggacata catatacggg ctccaggctg
      aacggctcgg gccacttaca
28321 caccactgcc tgataaccat gctggctgcc acagtcctga
      ccctggccct gctgggcaat
28381 gcccatgcct gctccaaagg cacctcgcac gaggcaggca
      tcgtgtgccg catcaccaag
28441 cctgccctcc tggtgtgtaa gtatcagtgc atctgtctgc
      cctgccaggg gtcttttcat
28501 ggacacccac tatgccagga gcctccctgg cctgaagcca
      gccctgaagc cggctgccac
28561 actagcccag agagaggagt gccctgggag ggagatgggc
      tgagYggagc tgtcatcacc
28621 ccctcctgac ctcgccttca aggtcaagtt ctttggtRag
      aaggtcctag ctgcattgca
28681 aacagccagg tatagggatt tgtgtttgtc tgagacccag
      aatcactggg gttcRagtta
28741 gggttcagat ctgagccagg ttagggggtt gagtcagggg
      gtaaagatta ggaggttggt
28801 gtatatttgg tgttgggggt cactctatgg ccaaagtcag
      gggttgccat gagctcaggt
28861 gacggaggct ccatcactga ctgttgtgtg actttggcca
      gctcccctgc cctctctggg
28921 cctcagtctc ttgctcaata taataagggt atagggaggc
      taaatgatac aatttctaaa
28981 atagagtatc gccaagttca aaagccagaa ttatagaccc
      ccaggactac agacagtgtc
29041 acagcatcgt ctgggtgagg ctagggttag tgtgcggctg
      ggctcagggc tgccccattt
29101 gctaggatcg tggggttccc atgtgtcagg atccagaggc
      tagggtatga tcaggatctc
29161 tagctggggt cagggtcaga gctctctgtg tcccctagaa
      ttgccatcaa ccttaaaccc
29221 agagggaggc ccagtccaac ccctcagctt taagacctgc
      tgggagcctc atctcagaga
29281 ggctgagtca tggccaaggc cagttggggg tgggagcagg
      gggcttggtg tgggcctgca
29341 gcccctcatc cactgccctc ccctctagtg aaccacgaga
      ctgccaaggt gatccagacc
29401 gccttccagc gagccagcta cccagatatc acgggcgaga
      aggccatgat gctccttggc
29461 caagtcaagt atgggttgca caagtgagtc gggcctcggg
      tgtgaccagg ctgggggtag
29521 ggaggcggga ggaacagcct ggggcttgcc cccagcccac
      agggaggaaa ggcagcagct
29581 gggggactca ggtctctccc cttgatttgg aaccagagcc
      tgacaccttc cctaccccca
29641 ccctccatac cctggtgccc tggggggatt tattggagtR
      tatcaacctc tccaacagcc
29701 cctctaagag tcaggcttca aagggtcctt tcccactgcc
      ctgggaagat ggaggtctta
29761 tttcggggtg aatggggggt agggtagaaa aatctcaaca
      aaataagtat tttttaaaaa
29821 aatgtataaa tgttgttttc ttatagtaca gacaggtctt
      gctatgttgg ccaggttggt
29881 cttgaactct tggcttagcc agtcccccac cccaagccta
      aaattagtat cttgacttta
29941 tttgggatga tggtaacagt caagggtcgg ttgtgggcca
      ggtgctttta caaacaaYat
30001 cctcaccttt agcatatatt tttttctttc ttgtttttca
      ttttttgaga cagagtctcc
30061 ctctgtcact taggctggag tgcagtggca caatctcagc
      tcgttgcaac ctccgtctct
30121 gggttcaagg aattttcgtg cctcagcctc cagagtagct
      ggaattacag gcacacgtca
30181 ccacatccgc gtaatttttg tatttttagt agagatgggg
      tttcatcatg ttggccagac
30241 tggtctccaa ctcctgacct caagtgatcc gcccacctca
      gtctcccgaa gtgctggggt
30301 tacaggcgtg agccaccacg cctggccctt tatctatctt
      aatctttatt ttatttattt
30361 atttatttat ttatttattt atttatttat ttatttattt
      attgagatgg agtctctcac
30421 tgtcacccag gctggagtgc agtggcacga tctcagctca
      ctgcaacctc cacctcccgg
30481 gttcaagacg ttctcctgcc tcagcctccc tagtagctgg
      gattacaggt gcccaccacc
30541 acgcccggct aatttttgta ttcttagtag agacggggtt
      tcaccatgtt gacctggttg
30601 gtcttaaact cctgacctca agtgatccac ccgcctcggc
      ctcccaaagt cctgggattg
30661 caggcgtgag ccacagcgtc cggcctatct taatctttat
      catatatttt agcctcttct
30721 gtaaaagagg ataatgccat cgcccggatg accctgctta
      acaaacacgg aaacaggagc
30781 cgatagaggt ttagcagcct ggcggagctc atgtgcaaac
      tcagctcagt atcggaaact
30841 cagccacctt catggcacct caggctgccc tggagcctgg
      gtcccggtct gtgtgtcggt
30901 ctgaggcccc aagggtgggg cagacattgt tcagggacct
      tgtctttgtc ttaaagccaa
30961 tctcctcctc ctccccggcc ctgctaaaca cgctccccag
      gggtggtctc tgctacccgc
31021 gcacattggg ccttgagtct tggtaaatgt ttctgagcca
      ccagggggcg ccagcggtgc
31081 tggatgccac ttcgcctttt gcaggaaaac tcccgccgct
      agcgggtgct gtggggcgtc
31141 ccaggtctcg cgcagccacg ccctctgtgt gaccttgggc
      ttcagtttag gcttctgtaa
31201 aagagaataa tgggcaaggt ccagtggctc atgcctgtga
      tcccagcact ttgggaggcc
31261 gaggtaggag gattacctca gccagggttc aagaccacct
      gtctctataa aaaatttaaa
31321 aattagctgg gcatggtggt gcgtgcctgc agtcccagct
      actcaggagg ccaggggagg
31381 tcaaggctgc ggtgagctat gattgcatca ctgcattcca
      gcctgggtga cagagcaaga
31441 ctctgttgct aaaaaataaa agaggataat gctatcctag
      acctatgtgt tgcaagaggt
31501 cagacatggc aaaagcgttg agaagttaaa gaaatagtat
      ccttaataaa tcagcaataa
31561 ttcagtaaga ccagactgcc atagactaga tgtcatagtc
      attaatatat aactgttatc
31621 ccaaacgtat atgagtgggg tagaaataaa atatgattgg
      ccataactat gattggtcat
31681 gattaatgtt agatgaagtt ggataatggg taagatgaga
      attcttcaca ctattctctc
31741 taaagcgaat ggaataaaat agaacagaac agaccgggaa
      agccagatat atgtggattc
31801 tccttgctgg gcacagtgtg tcatagtggc ttataatatg
      ggatttggag tgggatggac
31861 ccaggatcaa attcccttct actgaggttt tctgaagcca
      cataagctgt ctgagccatt
31921 tctgtctcag ttcagtgggt acagcaatag tgactacctc
      gtgggtagct gtgaggatta
31981 aaccaggaaa tgtgtataaa ctctaagcac agtgcctgcc
      acacagcatg tgtttggtac
32041 gtgatggccg cttctattgt gtcatattcc atgtgctcaa
      gccgggccct gggggagagt
32101 tcagcaaggg tagagggata tgagagtgaa tgtggcccac
      tggcctccct gcctgcagtc
32161 tctcctctct gatccttgct tcctaagggt cttcactgag
      ccccacctct gaccaagtcc
32221 ccggcggcac aggcagcaca gatcctctgc tgtacctaca
      ccaagacccc agggccaacg
32281 cttctcccat tgtgcagctc cacctctgcc cccggggcct
      agctgaggcc tcctccaagc
32341 tgagccacct actatcttcc aaactcccac cctccatctg
      gtgtcacctc ctcccagaag
32401 ccctctgtat cagtttcctg ttttctgcca taaaaaaaat
      cactccaagt ttagtggctt
32461 aacatgacac aagtttacca ttttacagtt taaaaggcta
      gaagtccacc atggccctcc
32521 Ytgagctaag atcaagctgt cagcagaacc agattccttc
      tgggggctat aggggagaat
32581 taattccctc gctttctgca gcttctagag gcttccccca
      ttccttggct tccctccatc
32641 tacttttatt tagggcgagg gaagcttcct gctctgactt
      tctcatttat ggtattctcc
32701 cctgctttcc agcagctgtg attgtcccaa gtcctgtcct
      ctggttctct aggccagaaa
32761 aactatgatc taccagagct caagaacttg aaagggcctt
      ttcccactgt cccttctcac
32821 agtgagagcc ggagcccagc ttcgtgctag aatctgtgaa
      aatggagaaa tgcaccctgt
32881 gctgttctct tcctccaccc ggcatctccc ctgcagcccg
      gctgggtttt tttgtttgtt
32941 tgtttgtggg tttttttgtt tttttgtttg tttttgagac
      agagttttgc acttgtcgcc
33001 caggctggag tgcaatggca cgatctcgac tcactgcaac
      ttctgcctcc tgggttcaag
33061 cgattctcct gcctcaacct cccgagcagc tggaaccaca
      agcatatgcc accactcccg
33121 gctaatcttt tttttttttt tttatgcatt tttagtagag
      atggggtttc accatgctgg
33181 ccaggctggt ctcgaactcc tgacctcaga tgatccacct
      gcctcggcct tccaaagtgc
33241 tgggattata ggcgtgagcc acggcctggc ccagtctgac
      tgttttcact tactctctaa
33301 tgtctcaggt agtgtttatt gtattctgtg cggaggtcaa
      gaatttagtc atccaaggag
33361 gttagtgtat taggagctta cgcagctatt accagaagag
      actccactca aaaatttggt
33421 acgtggctgg gcgcagtggc tcaaacctat aatcccagca
      ctttgggagg ccgaggcggg
33481 cagatcacct gaggtcagga gttcaagacc agcctggcca
      acatggtgaa accctatctc
33541 tattaaaagt acaaaattag ctgggcatga tggtgcatgc
      ccataatctc agctacttgg
33601 gaggccaagg caggagaatc actgaaccca ggaggtggag
      actgcagtga gctgagattg
33661 cccgctgcac tccagcctgg gcagcaagag caaaattctg
      tcacacaaac aaacaaacaa
33721 acaaaaaaaa cgggttgggc atggtggctc atgcctgtaa
      ccccaacact ttgggaggct
33781 gaggtggggg aatcacctga ggtcaggagt tcaagaccag
      cctggcaaca tggtgaaacc
33841 ccctctctac taaaagtaca aaaattagct ggatgtcgtg
      gcgggagcct gtaatttcag
33901 ctactaggga ggctgaggca ggagaatcac ttgaacccag
      gaggcagagg ttgcagtgag
33961 ccgagatcgt gccactgcac tccagcctga gcaacagagc
      cagactcagt ctcaaaaaaa
34021 aaaaaaaaaa caatggtatg agctttaaat ttctgtagaa
      ctgaaagagg attggagatt
34081 gtagtagggt ctaattctaa attagagcat ttagaataat
      gtggagtctg gtcaacatag
34141 tgagaccccc ccaatctgca caaaaaagac aaaaattagc
      caggcagtgt tggtgcatgc
34201 ctgtggtccc agctattcca gaggctgagg caggaagatc
      gttggaattc gaggctgcag
34261 tgaactgtga ttacacaact gcactctggc ctgggcaaca
      gagagccacc ctatctcaaa
34321 caagaaagaa caaactaaaa aaaaaaaaag aagaagaaga
      atttggagca atctagttgt
34381 ttcctatacc agaagtcttt gccaatctat ttttttttaa
      ttttattttt aatttttgtg
34441 agtacatagt aggagtatat atttatgggg tacatgagag
      tacatagtag gagtatatat
34501 ttatggggta catgagatgt tttgatatag acctgcaacg
      ttttgtcaat ctattttttt
34561 tttggcgaca gtcttgctct gttgcccagg ctggagtgta
      atggcgcgat ctcagctcac
34621 tgcaacttcc gcctcgtggg ctcaggctat tctcctgcct
      tagcctccaa actagctggg
34681 actacagacg cccaccatca tgcccggtta atttttttgt
      atttttgtgg agacgagatt
34741 tcaccatgtt ggccaggctg gtcatgaact cctgacctca
      ggtgatctgc cctcctcggc
34801 ctcccaaagt gctaggatta caggtgtgag ccaccacgct
      ggcccaccaa cctatttttg
34861 atctaacttt acctctggtg gtgtattgat actcttccga
      atcaattttc acccttcttc
34921 atataccatt tacatttcta agaatttgtc catttcattt
      catctagtta acctcatttg
34981 ttgacataca attgttcgta gtattctctc ataattcctt
      ttatttctgt aagactggta
35041 ataatggcct tgcttttatt tctgttttta gtaatttgag
      tcttcttctt tttttttttt
35101 tttgccagtc tagctaaagg tttgttgatt ttgttgatct
      tttggaaaaa ccagctattg
35161 ttttcaccga ttgttgtttt tgttctatta tttcattcat
      aggcactaag atcctgatta
35221 cttccttcct tctgctagct ttgggcttag tttgccctta
      tttttcaagt tccataaggt
35281 ggaaagtttg attattgagt tgagatcttt ctctttttta
      aagataggca tttctagata
35341 taaatctccc tgtgagcacg gttccctcca tcttcagcac
      accagggttg actctctccg
35401 ggcgttcttc cctggtcacc tctccccttc ctctcctctt
      ctgcctcctc ttccactttt
35461 cggtaccctg tgattgtatt gggaccaccc agataaccta
      ggatcatctc cccacctacc
35521 ccaaggtcct taacttaacc atactttcat atgggtaaca
      cgagttgagt gtggtaccca
35581 gggtttgaca tgttgggtaa catatttgca ggttctgtgg
      attaggagga cattttgggg
35641 gccatgattc tatcttccac cctcgcctag acaaaattgg
      aggctcactc cttgggctcc
35701 ctggatgacc cccaacatcc ttcctcactt ccattccttc
      ccagcatcca gatcagccac
35761 ttgtccatcg ccagcagcca ggtggagctg gtggaagcca
      agtccattga tgtctccatt
35821 cagaacgtgt ctgtggtctt caaggggacc ctgaagtatg
      gctacaccac tgcctggtgg
35881 taagcattcc tgtcagctga tgccccatgc cctggccctc
      tctgggctgg agggctgaat
35941 gagggtcctg ggtccttggc tctttccagg ctgggtattg
      atcagtccat tgacttcgag
36001 atcgactctg ccattgacct ccagatcaac acacagctga
      gtatgtgtca agcgtcctct
36061 ggggaagtgg gagctggact ccagggcttg gcctcagcag
      agggggaggt tgtgcaggca
36121 gagggttctg gggccaccaa aggaggccca gcctgggaag
      tttgcagggK tggggacccc
36181 agagctggcc aagctcttga ctggcctggg cagcatgtgg
      ataccatctg atagcggagg
36241 ctgccctgag gtcatgtcgg gtctccctgc agcctgtgac
      tctggtagag tgcggaccga
36301 tgcccctgac tgctacctgt ctttccataa gctgctcctg
      catctccaag gggagcgaga
36361 gtaagtacac caccctgtgg cccccattcc tgctcgtgcc
      catcctgtta gtgtgtccac
36421 ggctccYtcc aggctcaacc ccacacaggg catgcttgtg
      ggtggccaaa cctgagggca
36481 gcaatacctt cagtggggtc acttcctacc ccctcccatc
      aatacaccct caaaggctgg
36541 aaacaacaat aaccaacagc tagtaactaa cagctattaa
      gaacttgctg tgtgcaaagc
36601 actattccaa gcccttttca tgaattaatt gattttgtcc
      ttaaaaccaa ccctaggata
36661 tagattctgt tatcatcccc tttttacata tgggtaaact
      gagtcacaga gaggttagaa
36721 aggaaaagct catatctacg gagtgcatcc tgcattccaa
      gcaccacact aactcagaga
36781 taaaactcta gccaagctaa gtaacttgct gaggacacac
      aactcgccac taagggatgg
36841 gagtaggatt tgaacccagc attctctgac cccagaagct
      gagttcctag atactttact
36901 ctcctgcttc ccagggtggg gctttttgtc ttggccaaca
      ccctctgtca aggagctgtg
36961 ggtaacccca ttgcacagag gaagataaca aggtttggag
      agtccctagt catgttacca
37021 atgccaaacc tggaaggcag aagggaactg gtgggtgggg
      tctggagagg agccctctat
37081 tcaggccatt ttctgactct ggagcagacg gatacatgta
      tgaatttgga ctctagacac
37141 gttctcgtgt gtgtgacagg tgtgagcgtc acaggagctg
      ggccctcccg aggaattctg
37201 ggatggtgcc acagttaatt cttgggtctg aggctccgtg
      ttctcagctg caaaatggga
37261 gtgataattc ttacttcctg agctacaaga gtcagggcca
      acagagccat gaacggtgcc
37321 tggtacacac taggcRctcc atggatgcac aggactggtc
      aggggctcat tgtggtgctt
37381 gctgccttca ggcctgggtg gatcaagcag ctgttcacaa
      atttcatctc cttcaccctg
37441 aagctggtcc tgaagggaca ggtgagtgag gctggctgac
      tccctgtggt ccagggccat
37501 gcccaggagg ctggatccct ttcctccctg cctttccctg
      agaaggtgcc actcccacct
37561 tctccatgtg gccagtcccc tgtgccggtc cccagcactg
      ccaccaccac gcagctggaa
37621 ggaggcactg cctctggcct cctttcctgc ctggaaagca
      cctgctctgt ctgccccaga
37681 tctgcaaaga gatcaacgtc atctctaaca tcatggcYga
      ttttgtccag acaagggctg
37741 gtgagtgYgt ttctgtctgc atgcctcaga agacagcagt
      gggagccaga aagccacctg
37801 ctgcactatg tggccttggg actgtcactc ttcctgtcta
      ggtcccatgg gctctatctg
37861 gctctgacac ttgatgatta gttatgagca tactttggca
      aatctctgcc cctttgggct
37921 gcagcMtcac aagctgtgtg gcgttgggca agtctataga
      actcaggaca aatgggtgat
37981 taagtccaag aggactccaa gattctcctg gaagtagatt
      aggaaaaaag ataattagat
38041 tgctcacatg gctgggcact catccatgta ctgtactctc
      ctatgcagta cagagcagag
38101 ctgggtttca gcccaagtct tggactctgc tctgaaccaa
      ccttctagaa gggctctacc
38161 tacccagaca gacagacttg ggaaaagaga gaatgaaaaa
      gtgccacacc cctccccgca
38221 cacccaggtc ccactttaca gaggggaaca ctgaggctgg
      agggttgggt agctgtgtgg
38281 atgcagggga gcggtgactc agggcaattc ccccatcctg
      aggccctgcg ttgatctttt
38341 cctcctgcag ccagcatcct ttcagatgga gacattgggg
      tggacatttc cctgacaggt
38401 gatcccgtca tcacagcctc ctacctggag tcccatcaca
      aggtaggagt tgtgggaggg
38461 tggggcaggg cccagcttcc ccaggggagt tggtcctttt
      ttgtgctctg acaaccccgt
38521 cccccagctt caaccttatg gcagccaaga gtcctggggc
      gctcctcctc attcctgatg
38581 ctcctccgca ttcctgatgc tgcgaggagg gcaggccaca
      gcgacgtgcc cctgacccct
38641 ctctgcaggc accagggctg cccactacaa ggatcccagc
      aaagcaccag ctccttccta
38701 gagggcttat tcggcttctg tcatcctcta cagcagtgga
      ttgtggcccc cYcccagggg
38761 gtactgacaa aagctttgga ccctctatta cttaggatat
      agatttctgt aacaaagaaa
38821 tccgaaataa tagctgctta aacacaataa acagttattt
      ccctctgttg cgttctgagc
38881 atctgcagcc ccgcgggatc aggaggctgc agggtgtcag
      gcacgcaggc cccttccacg
38941 tgttctcagc cagccccagg catgacctca tcccagggtc
      ctggctgact ggcctgacca
39001 ccacacccac tctgctcaca gcccgttggc ctgaacYtga
      tcgcaggacc ccaccctagc
39061 tgcaagggaa actgaggaat ttatttcagc taaatattga
      gaccagctaa aagttaagtg
39121 gtacagaatg gagggagaga ttggagaaga ctttagggcg
      gacccagcca tctttgtaac
39181 agagcctctc cctggaaaac tggacagaag gatcatttca
      gaggttcatg gccactcctg
39241 aagcctgttc acaacccatg ggctggatga tctagtgggg
      cgggggactg ggcagcagct
39301 tgttttcctg attttggcct ccaggagccg atggtcaatg
      gactgccctc tgcaggggca
39361 gggctggtgg tcagctgggg cggggtggga gctggaggtc
      cgtggtcacc agctgccctg
39421 actaatgtcg ttacttgaat ataaccctgt gaaggcagga
      accacgtctg tctggttcac
39481 ttcccacggt ggttgagaca tagtgggcac tccggaagta
      tttgttgaat gagtgaaagc
39541 cccgctgggg gaaactgggt acagctcttt cctcagtttc
      cccatctgca ctctgggctg
39601 aatgctgggg ctcctcccaa tctccctgaa gctggacctg
      agcccagtag ggacacacag
39661 ggtccagcca gcgtcctggc ttcctccagg gtcatttcat
      ctacaagaat gtctcagagg
39721 acctccccct ccccaccttc tcgcccacac tgctggggga
      ctcccgcatg ctgtacttct
39781 ggttctctga gcgagtcttY cactcgctgg ccaaggtagc
      tttccaggat ggccgcctca
39841 tgctcagcct gatgggagac gagttcaagg tgagtgggtg
      gggctgggct gctagggRat
39901 ccagatggca tgtggtatgt gtgtgtgtgc acacgcatgg
      ggaggaggga ggaaactcgg
39961 aaacttggtg gtgggcaaaa gaactaagct ggagcaatag
      cagtgaagtc cagactgggc
40021 acagtggctc acacctgtaa tcccaatcct ttgggaggct
      gagatgtagc aggacgaacc
40081 gcagacaaaa ctcctcagac actgagttaa agaaggaaag
      agtttattca gccgggagca
40141 tgggtaagac tcctgtctca agagcggagc tctccgagtg
      agcaattcct gtccctttta
40201 agggctcaca actctaaggg ggtctgcatg agagggtcgt
      gatctattga gcaagtagca
40261 ggtacgtgac tgggggctgc atgcaccggt aatcagaacg
      aaacagaaca ggacagggat
40321 ttttacaatg ctctttcatg caatgtctgg aatctataga
      taacataact ggttaggtca
40381 ggggtccatc tttaactacc aggcttaggt caggtaggcc
      caggcctggt ttcgggtctg
40441 gttccttggt ttcgggtctg gtctctaggc gttgggctac
      ctgcctttag tttcgcttct
40501 ctttcttttt ctgagtataa aacaatataa aacaatatga
      gagggtctgt ctctcttctc
40561 tcagagacag gagggtccct ggaggccagg agttcaagac
      cagcctgggc aacatgggga
40621 gacccctgtc tctacaaaaa taaaaataag tttaaaaatc
      agctgtgcac ggcRgtgcat
40681 acctatagtc ccaactactc aggtggctga ggagggagga
      tcacttgagc ccaagaggtt
40741 gaggctgcag tgagctatga tggcaccact gcactccagt
      gtgggtgaca gagtaggact
40801 ccatctcaag aaaaaaatac agtccagcat ttattggagt
      caactatgtg ttgccagata
40861 gatagatgga tagatagaca gagtgatcaa tagatttagg
      tatagatata acttggcacg
40921 tagtaactgt gcattaaata taagttgata ttagacttac
      atttattgca gttatatttg
40981 ctacactttg ttttgttgca ctcatcctgt gaaataagta
      ctgttacctc tgttttgcaa
41041 acaaaaaaac cacaaagctc agagaaggta ggtgacttac
      tgaagatcac acagcctgta
41101 agaggtggcc ccaaagcctg tactcttcac ctatactgta
      ctagaaatgc ttaggtagtt
41161 tccaggctgt gcttgtcatt gaactcatga ggaaactgag
      gcccacagag gggaagagac
41221 ttgtgcgagg tcacacagca tgtgtggggc acagtgggga
      gcagaagcca ggcctccagc
41281 cgggacaggg gttccctgtt ccacaccctg cccagagcat
      ctcacatgtt gtctgggagg
41341 ttgggagttg cgtctgagga ggggtccagt ccttgaaact
      gcccttggtc cctgcgaagt
41401 tttcttctga ggagtggact ttactccacc caccctccaa
      cttcctcatt tcttttcagg
41461 cagtgctgga gacctggggc ttcaacacca accaggaaat
      cttccaagag gtaactgccc
41521 cctgcccctg tgtggggttt atctcacgta ccccaatcct
      gctctggctt caagagcccc
41581 cacacagcca ataacaccac caatggcaac aattataaca
      gcgaacacag ctcctactcg
41641 gttgttcggc atggagtgaa gcacttagtg tgtgtgactt
      ccttcagtgc tcacaccgac
41701 cctatgagtg gggcggtcaa actgtcccca ttttacacac
      agggaaactt agtgaatggc
41761 aaggctgggt ttgagcccag ctctattgcc cccaaagata
      aggctccatt ccctgctcca
41821 tttcccaggc atagRgactt gtagggggct ggaaccccag
      gatcaactct gggctcagag
41881 ggccccagca ataagtgact gttgattact cctgatccca
      aagctgactt caggcaagct
41941 ccttggaggt cgcagcccct tcttgctatg cccagtggca
      atgatgttca taatcccact
42001 cctcagtgca gggttccact aagaacccat gatctcctac
      ctcaaatgga cctcatgctt
42061 tctgagtaag cctccctcag ctttctggtc acctcactcc
      ccccacccac tgcaatgact
42121 tcttcaggcc ttccctgcca tcctcaaatc tccagctgcc
      ccctcctgtc taccttccac
42181 ttccctctcc acacacaacc tgcttaccag agagctgagc
      agagccacca acagaacttc
42241 ccccccacgt cgctgctccc agtcgcaacc cactcaccca
      cacctgcgcc ttcctgcggc
42301 cgttctcctg ttctaacaga ggacRgtccc tcttcccttc
      tgaactcagg ccgtccagcc
42361 ttccccggct ctgtgatgYc tctctccYtg cacatacaca
      actcacaata ttgggcaggc
42421 ccctgaccgg cYcttctccc ttagcccctt gtctgagtcc
      tgttcctttg atgtcctatt
42481 tctagtcttt ccatttctct ctgtctctac ctcctcccca
      ggctaccatt atctctagtg
42541 gggacccatc tgtagctccc ggtgggtctc cctgtgtcct
      gataccctcc aataatctgt
42601 tccccacagc agccagaaca atcttttcaa gaaataaaac
      tggtcattca cctgcctgct
42661 taaagccggt gtaggataca ggctaaacgc tctcccatgg
      cctccagggc cctgcatttt
42721 ctcatcctgt ctacttcttc atctagttgt ttcccaccca
      gcgtaattgt aactgtttag
42781 ttgaaagttg acgaggtctt ttgtcagctc tagaaatttc
      ttttcttttc tttctttttt
42841 ttttttttaa gacagagtct tgctctgtgg cccaggctgg
      agtgcagtag cacaatctca
42901 actcactgca acctccacct tccgggttca agcaattctc
      ctgcctcagt gctgaaatta
42961 caggtgtgag ccacagcacc cagcccatca gctctggaaa
      tttcttcatc actctctctt
43021 ccatgattgc cccatcccta ttttctccat gcctacctcc
      tggaatttcc atgagacaca
43081 cgttgggcca tctcattcca ttttccgtgt ctcttagttg
      ctctgtcgtg ttttccatct
43141 cttttatctc tatcctaaat tctggatagt ttcttcaagt
      atctttttag ttcattaatt
43201 ctttttacag ctgtgtctta tttgtttact tacccactga
      gattttcatt ttattttatt
43261 taattaatta atttatttat ttttgagaca gagtctcgct
      cttgttcccc aggctggagt
43321 gcagtggcgc aatctcagct cactgcaacc tacgcctcct
      gtgttcaagc aattctcgtg
43381 cctcagcctc ccgattggct ggggttacag gggtgcacca
      ctgtgcccag ctcatttttt
43441 tgtattttta gtagagacgg ggtttcacca tgttggccag
      gctggtcttg acctcctgac
43501 ctcaaatgat cctcctgcct cagcctccca aagtgccggg
      attacaggtg tgagctcccg
43561 caccccgccg gccctttctt tcttttgagc tgagctatgt
      atttaatgaa accttttctt
43621 gtgtatccag cRgttcagtg gtttcttggg gcatgtgatc
      tccttgagcc cagtcccaca
43681 ggctgtaatc gaaggtcctc aggccacact ccccagcccc
      gctggctgcc tgcgcattcc
43741 ttatcttggc catgttcctt ccacagggct cttgtccaca
      ctgtcccctc tgccagaatg
43801 gtttgccctc ccccatgcca cctgctcctc ctcctgccac
      ctcagggaag gcctctccca
43861 cctccctgac taggtcaggt cccctcgtgc cacagcctRg
      tgtgtctctc ctttaaatac
43921 tcctcagctg cagttttacg ttctgttctg ggatgccctg
      gaaaatgctg ggctcctgca
43981 gatatggccc gcagctcctg cgctctctgc tctatcccca
      cccctagccc agctgctaca
44041 gcagcttccc tcggagggtt accatgcagt aggtcctgtt
      ctaagctctt tccacagatt
44101 atctcattcc atcctcagga caaccctatg aggtaggatc
      tatgattatc cccattttac
44161 agatgaggaa agtgaggccc agaaggctaa gtgaaggcat
      cctcagcaca tcctgagaga
44221 ggagttcagg gtaggaatag cttcactagc acacagatga
      gaaactgagg ccYagagagg
44281 acaagagtcc taatttgcac agcctggggc ctgggcacta
      ccccgagcta cttccttttc
44341 cccagccctg gggcccggag ccagctttgt ccttcccatc
      tccgagggca tggactgctg
44401 ctgccctgat gggcccctgt cctggccatg ggacccctgt
      cttccacagg ttgtcggcRg
44461 cttccccagc caggcccaag tcaccgtcca ctgcctcaag
      atgcccaaga tctcctgcca
44521 aaacaaggga gtcgtggtca attcttcagt gatggtgaaa
      ttcctctttc cacgcccaga
44581 ccagcaacat tctgtagctt acacatttga agaggtgagg
      cgggtgcagg gagaggtggt
44641 ggtgggggaa cctgactcac atatgggccg cagagggcag
      gggcctgggg gtctctgaag
44701 cctccagatc cttctcacca cctctgctgg cactggttgt
      ctcttgcaca tggctcctta
44761 caatcaaaat cacatcatgc aagtaacgag ggggtacaca
      cgtggtttcc acagcttagg
44821 taatattttc tctcttttct tatttttatt ttttttagag
      acagggtctc actctgtcat
44881 ccaggctgga gtgcagcctc acactcatag ctcactgcag
      cctcgaatcc tgggctcgag
44941 tgatcctccc acctcagccc tcctgagtag ctgggaccac
      agacaaacac caccacacct
45001 ggctaattta aaaaaaaaaa tttccttaga ggcggggtct
      tgttatgttg tccaggctgt
45061 tctcaaactc ctggcctcaa gcaattctcc tgccttggcc
      tcctaagttg ctggggttat
45121 aggcatgagc caccatgcca agcatatgtt ctttcattct
      tatttttatt tattatttat
45181 ttatttactt atttattttt ttgacacaga gtctcactgt
      gttgcccagg ctggagtgcg
45241 gtggcacaat ctcggctcac tacaacctct gcctcccagg
      ttccagcgat tctcatgcct
45301 cagcctccca ggtagctggg attacaggca tgcgctacca
      cgcctgacta attttttgta
45361 tttttagtag agacagggtt tcgctatgtg ggcccagctg
      gtctgagact cctggtttca
45421 agtgatctgc ccgcctcagc ctcccaaagt gctgggatta
      caggcatgag ccaccacgcc
45481 cagccaatat tctcctcctt aagctgagca gtggacaatg
      gtgtttatta cccgagtcca
45541 caacccttta ttcattaggg ccacaagggt ttccaaattc
      agaacttctg tgaatttgta
45601 aaagtagtat agtgtataga cactggatat attgcagaac
      accccaatag ggtgtggggt
45661 ggcaccccac aatcaattct cctcaaatgc ctcaattggc
      atttctcctc atatgaagca
45721 agataaatac catacatagc ttcctagaca atggccaggt
      tttgccaaca aaacttaaga
45781 aaaaacttgt agttttcaga tcctgtttga ttttataatt
      acaaataagg aattaagaat
45841 cagtattatc tttaatgctc cttagaatat cttcaatttt
      tttttttgag acagagtctc
45901 actctgtcac ccaggctgga gtgcagtggt gcaatctcgg
      ctcactgcaa cctccacctt
45961 cctggttcaa gcgattcgcg tgcctcagcc tccccagtag
      ctagctggca ctacaggcgc
46021 gtgccaccac gcccagctaa tttttagtag aggcggggtt
      tcaccaggtt ggccaggcca
46081 gtttcaaagt cctgacctcc agtgatccac ctgcctcagc
      ctccaaaagt gctgggatta
46141 caggcatgag ccactgtgct cggccaatat ttcttaatac
      attaagaact aaagacaaga
46201 gcgatacaat cacattgtgg aaaatataga aaaatagaaa
      gaagaaaacc actgtcacca
46261 taaccccctt ctccaggggc cttcgatgtg gtgactgggt
      gcatttcctt ctggtgcttt
46321 gttattttta aataaatttt ttatcttgga gtaactttat
      atttgaataa aagttgcaaa
46381 atacagagtg ttcccatata tcccccaccc acttcccgct
      ttgtccacat cttacataac
46441 catgctacag tgtcatagcg gagagattaa cattggtgca
      tgactgttaa ccaaactcta
46501 aactttattt gaatctcccc agtttttcca ctgatgatac
      cctttttctt ttccaagatc
46561 tgatccagga tagcacattg tatttagccg ttcttttttg
      ttgttgtttg tttgtttgtt
46621 tgtctgtttg tttctgccca ggctggagtg cagcggtgat
      cctcccacct cagcttctcg
46681 actagagatg tgcactacca cgtatttttt tgtagagaca
      gggttttgcc acattgccca
46741 agctggtctc aaatccctgg gctcaagtga tcctcccacc
      ttggcctccc aaagtgttgg
46801 gattataggc atgagccact gtgcccagtt tatttagcct
      tttaaatatc tctttaattt
46861 ttgtattact ttccttttca tttttctgtt ttattccttt
      tggctttctc atgtggatgt
46921 agacatatta ttttatttac ttattttttt gttgttaaga
      atctgaaaca ttgagcattt
46981 gttaaaaaaa agaattattc sgccgggcac ggtggctcac
      gcctgtaatc ccagcacttt
47041 gagaggctga ggtgggtgga tcacatggtc aggagttcaa
      gaccagcctg gccaatatga
47101 tgaaacccca tctctactaa aaatacaaaa attagccgga
      catggtggca catgcctgta
47161 gtcctagcta cttgggaggc tgaggtagga caatcgcttg
      aacctgggac gtggaggttg
47221 cagtgagctg agatcgtgcc actgccctcc agcctgggca
      acagagtgag actgtctcaa
47281 aaacaaaaaa agaaaagaaa agaaaaagaa agtgacttct
      caggtcctaa ccccaaagcc
47341 acaggtgctg gggaactttc ctcggttttc agaagagcag
      tagctaagcc tggttcccgt
47401 gtcatccttg cctctccagt ccctcagtgg aaagaatcag
      gggccctgag ctaggagggt
47461 tgctctctgc ttcgggaaga gccctggctc acagcaaatt
      tggtttctct ccccaggata
47521 tcgtgactac cgtccagScc tcctattcta agaaaaagct
      cttcttaagc ctcttggatt
47581 tccagtatgt gctgcagaga agagaggggg cggtcaactc
      cgcaaacctc tccctggccc
47641 cttggagtca ggcacagggc ggggtgttgg tggggaaatg
      tggccccttt cYtctggggc
47701 atatgggctg actgcaggga gataagaccc tgcctagata
      gaatcttcgt ggggaagaag
47761 gggctccagg aagaatggag ggctgccagg aagaagggcc
      tggcaggagg agagcgctgc
47821 ccgagcaaag gcctggccgc cagaatagca aatctcaagg
      gaatagcaaa tctcaagaga
47881 gtgccccaaa gggcctgagc tatgagacag aagcactggc
      tgctattctt agagtttctt
47941 tcccagggga tgttacagga gggggcccaa tggagggtca
      aattatcatc gcttttttat
48001 ttcaggatta caccaaagac tgtttccaac ttgactgagg
      taggtagtct tggatagact
48061 gggggaaata agtcctgtgg gacctcctgc cttaaagaaa
      gcaggcggag ggccctaaag
48121 gaaatcaggc aaccagacca aaagaatgtg gaccaggtgg
      tccatgctgt gtctcttgtg
48181 acccttcttc tccctgccat gtcttttggg agagcccttg
      tgttgcaaaa atgagagtgt
48241 ggtggtatgg attggggttt aggcagaaca gtactggcca
      agcagcgcct ccctggacct
48301 caattttccc tctgtggaat gggctagcaa tcctgggcct
      ccccagggcg aaggaaagac
48361 cactcaggaa gggcaccgtc tggggcagga aaacggagtg
      ggttggatgt atttttttca
48421 cggatgggca tgaggatgaa tgcttgtcca ggccgtgcag
      catctgcctt gtgggtcact
48481 tctgtgctcc agggaggact caccatgggc atttgattgg
      cagagcagct ccgagtccRt
48541 ccagagcttc ctgcagtcaa tgatcaccgc tgtgggcatc
      cctgaggtca tgtctcgtaa
48601 gtgtgggctg gaggggaaac tgggtgccga ggctgacaga
      gcttcccatt tcaccttgtg
48661 ggcccttccc aggcagagct tcaggtgccc ctcttcccag
      tcattgatac ttagcggtcc
48721 tggccccctt tcctctccct gctggtggYa ttgcacgcca
      atgactcggc cagatgccca
48781 gacccctgtt cttggtttac ctgcagaata ttatctttgc
      caccccgcgg gatggctcaa
48841 cccactttca ggatgcaggt ctcctaatag caacctgata
      tagcagaaag acccctgggc
48901 tgggagtctg agacctagtt ctagcccagc cctgaacctc
      agtttccctt tctgtgaaac
48961 aagaatgttg aacttgatga ttcccaattt tccttttgac
      cttgaaatgg tagaatattt
49021 atccctttga ggtgactcgg atggtagact ctcagacacc
      atagcacacg tgtgctgggg
49081 gtattttgga ccaggctctg ctgagagctt tctgctccct
      tccccacaga gtatgttgag
49141 ctgactgtgc tttccttttg aacatctgct tgtccacatg
      gcttaggtag gagaggaagg
49201 gcgtggaaac tggaatgatc ctagtggggt gtcttggcat
      ctcttggcct cattttcccc
49261 atctgaacca tgaagctaaa actaggggat gtggattaaa
      tggttcctac aactacttgc
49321 aaggagacca ctctgtgtgg ttgcaaagaa cactttgaga
      agctgtgtgg gaaagtttcc
49381 ttcctagcag ggtagactca gctaactgca ggtcatgtgg
      ccattgtgga tgggttggga
49441 gctcaagttt ggggcagaag ggaatttttt ttggcagcag
      agtggcaagc cctgccgcca
49501 ggcaaactct gctcttcctc atcctcagaa gcacttgctc
      actctgctaa atcaaagtga
49561 aacgcatgtt tacagaatat tggtccaaaa gggtctcagc
      atctcccact acccagggtg
49621 gcagagcctc gggccggcct tgctccccaa gaagggctga
      ctggggctct gtcccctgcc
49681 ccagggctcg aggtagtgtt tacagccctc atgaacagca
      aaggcgtgag cctcttcgac
49741 atcatcaacc ctgagattat cactcgagat gtgagtacaa
      agcccccctc accagcccct
49801 gttcctgggg agagaggccc agacaggatt cctggggtga
      ctgggggctg ttggggagac
49861 agacagaggg gcctctacca gcttggctcc ctcctggtgg
      cctgggagtc agcccagctc
49921 Rcccctctct cctactgccc ctcccttcag ggcttcctgc
      tgctgcagat ggactttggc
49981 ttccctgagc acctgctggt ggatttcctc cagagcttga
      gctagaagtc tccaaggagg
50041 tcgggatggg gcttgtagca gaaggcaagc accaggctca
      cagctggaac cctggtgtct
50101 cctccagcRt ggtggaagtt gggttaggag tacggagatg
      gagattggct cccaactcct
50161 ccctatccta aaggcccact ggcattaaag tgctgtatcc
      aagagctgSg gagtccttct
50221 tctgtggctg gcgggtagag ggRgggggaa gggattgtct
      caccagtgcc gtccacctct
50281 tttcagccct tccaaggcag ctgcccccaa accctccaag
      cttcatgatg actggaggaa
50341 gaaatccaaa cttctctcct tgggactcac gatcctccct
      gatcaggtcc ctggatacct
50401 tccaaattta tcccctttaa cccagcactc tccttgtctg
      accagcttcc tgcaggagct
50461 gtcacacact ctctgtccct tggggttcca gtgctggaca
      gaaagtgagg gaggaccagg
50521 gcttcagatc ctgaggatgg tgggagggga aggggccctc
      agaggtctgg tttctggtag
50581 aaatggagga agacagagcc tttaaaaagg caaacgactt
      tattccagaa gataaactgc
50641 tcacgagcct gagcttattt ccagctcacc tttttttttt
      tttttttttt tgagacggag
50701 tctctctgtg acccaggctg gcatgcagtg gcacaatccc
      agctcactgc aacctccact
50761 tcccaggttc aagcaattct cctgcctcag cctctcaagt
      atttaggatt acaggcaccc
50821 gccaccacac ctagctagtt tttgtatttt tagtagagac
      ggggtttcac catgttggcc
50881 aggctggtct cgaactcctg acctgcagtg atccacctgc
      ctcaaccccc caaagtgctc
50941 atgttaaata tggtgaaccc caagtttttc ttcaaagaat
      cagtatgtct gtatgttcag
51001 ctctcttatt ctttgattct ccattttaaa gtttaacttc
      ctggttctct tcgccccctt
51061 gcttctaatt tcagtaaaca accttttcca tcagttttat
      tcagtagttc acacctgttc
51121 ccctggtcac ttgctccatc ctgactcatc ccagtcacct
      gctttgacct gagtcacccc
51181 tggtcacctg ctctgatgta agtcaccttt agttacccgt
      tcctaactgt ccttcctgcc
51241 aaactactca cctcgccact ctggctcata cctctgctct
      ctttaaaata gccaatcgga
51301 attagattag accgtgcggt ccaaccttag ccaacagggg
      aacaacatag cagcagggat
51361 tacctgggtc aggaataaga accccttccc ctcccttgtt
      caactgtgct ctcaccattg
51421 ttccatctat gaggagcacc ctttctacag aaactaaaaa
      ttgcttgctg agaaaattaa
51481 atttatgttt gagtgctatt tctttgcagc agtggagaac
      aagcattcct aacactggga
51541 ttgcaggcat aagccaccgt gcccagcttc cagctcacct
      tcttatgctc aaattgtgtc
51601 ttctccttcc caccctcaag ctacaataca aaaagtttct
      tccaaccctc tcagtcttgg
51661 aaggtagcag gctctatttt ctctgggtgt gtaaagaaaa
      tgcctctccc acattagcca
51721 attgagacgg gaggaagcgc agcaagatgc tggctagtgc
      aaggtccctg gggtagaaag
51781 aatcatgatg gattcaagaa cagtcaagag ggctggtgtg
      gccctgaaag caaagaacta
51841 ggggcgagag atgaggccca accagcaggc agggtcacat
      aacaaaggtg ataaaagggc
51901 ttggccttta tttcagcagc caggagagac agccaggagg
      ctggggctgt ggggagctca
51961 gaagtaagat gccccttcca gtctgggaat atgtgagggg
      agggcaccat cttctgagcc
52021 aggtttcttc ccagcccttc cctgcccttc caagtgcttt
      ctgaggcctg tttctcttcc
52081 tcccctgggg cctaatgcaa gtgtctgact cactaagcag
      gagcatcatg ggcgggatgg
52141 gcggctctta aagggccctg gcagaaggag tcagtgtgat
      ctcgccagtc cctgagtcca
52201 acgtgggaat tctgggtctt gggatattgg cctttgaggt
      ggtgacacca gttaggagcc
52261 acatcccacc caagaatgtc cccttgaaga ggctgggaag
      gcccaaggcc cccaccttga
52321 tgctacaatg acagaatcag gataacttgg cagccctaag
      gcagctcttc cttgaatgga
52381 aacttttatc tgcatgaggg gcagacagag aagtcacttg
      gagtcttaac ccttgtagac
52441 cccagcctga ctgggactcc agaaggtccc agggctctgc
      tccagaaaga agggtagggg
52501 catgccccca gcttccttca gtgaccttgg agaggtctcc
      ccagggctga gctgggaggc
52561 acttccatgt cactgtctca tccaaggagt gcgattagca
      ggagatatgg agtccagagg
52621 gtgagaggct taccaaaatg ggactgacct cYgagcccct
      ctcccaaatt ccagcccctc
52681 catccaagcc tagaagctct gacctagtcc tattgaagtc
      ctcctggcct gggtgtgtgt
52741 tggggggaat cctggctcca acccagttag gatcatttcc
      ataaagaagg ccacacctgc
52801 tgcttcctct ggggaaaaac aagtgctccc caaggccagc
      accaggcaga agcacatcct
52861 gccacctggg ctgctgacct tgggcaggat ggggcccggg
      tgtttggcaa ataggaaggg
52921 tagagggaag gcctggcttt cagacctggg tctaatccca
      gctcccccca accccagtta
52981 ctaattctgc catcctgggg cccctctgtg cctccatttc
      ctcttctatt caatgggatg
53041 ataatacttt ccatgtagga ttctggcaag ggctgggtta
      gcccagagga ggagcccatg
53101 gtggccaaga gcaggggctg cagagctggc ctgcctgggt
      ccacccttct gctcctctgc
53161 ttctgtgtga tcttggccaa attatttagc ctctctgtgc
      ctcccttttc tcatctgtaa
53221 aaagaaagat aacagttgcc atgaagagta aaggagaggc
      cgggcatggt ggctcagacc
53281 tgtaattcca gcactttggg aggctgaggc aggggtatct
      caggagttca agaccagcct
53341 ggccaacatg gtgacccccc ccatctctac taaaaataca
      aaaaaattag ctgaatatgg
53401 tggtgtacac ctgtagtccc agctactctg gaggctgtgg
      caggagaact gcttgaacct
53461 gggaagtgga ggttgcagtg agctgatatt gtgccactgc
      actgcagcct gggcgacaga
53521 gtgagactgt cttaaaaaca aacaaacaaa aagattaaag
      gagagagcgg ctgcattttg
53581 ggaggccaag gcgggctgat tgcctgagct caggagttgg
      agaccagcct gggcaacatg
53641 gtgaaacccg gtctctacta aaatatgttt ttaaaaaaaa
      ttagctgggc atgcacctgt
53701 agtcccagct actcgggagg ctgaggcagg agaattgctt
      gaaccggaga ggtggaggtt
53761 gcagtgagct gagatcatgc caatgcactc cagcgtgggt
      gacagagcaa gactccatct
53821 caaaaaaaag gagagagcac agtgtctggt atatcctaag
      tgctccacga gtgccagaca
53881 ttggttatac ttcatggagg tgaggtacac agaggtgtct
      aggtcatgcg gactcactcc
53941 atcctccagt taaccatgac tccctcccca cccacctcct
      gagttctctc tgctgtcaaa
54001 ctttcctctc atatctccag tttcccaccc aaaagtccaa
      acactcaaga ttccttctca
54061 tctccaaccc ataaaatctg atcggcttct ccacactcaa
      gtgtaaatga ctgatggcca
54121 tttgattgac actggagggg ctggcacatt tttagcacat
      ttttagccaa aggtatgatg
54181 tgctgatagc ggactctcag gccacaagcc agtctgagga
      cagggaaagt ttgaggaggt
54241 cactcttcca gaaccctttg gtgataacct ttctggcagg
      cctggtaccc ccagagtgaa
54301 aagggctcca gggtcaagac ccagctctgc ccctcattgc
      tgtgtgacct tcagcaactc
54361 acttgattta tctggacctc agcttcttca tctgtctaat
      gggataataa tctttgtcct
54421 gccctcctca cagggctgtt cagaattaaa tgcatctgaa
      aatgatctgc atttgtgtct
54481 tggactgtgg ttatttgttc aagtgtctgt ctaccctgcc
      tgcacaggga gcgggtcagg
54541 gtcttccctg ggacttcatg cacaggtctt tcctgggact
      tcatgtagga ccagccatcc
54601 cgtcagtgct cagtgaacat gagctgcttc cctgtgggat
      gtctgggagg tgagtggaag
54661 gccttcctag caggcacata ctggaacata atcaatccct
      cccatgcata cagctcacac
54721 ccacattctt atccattccc accatgcccc tcagtggcct
      ggggagacta ggaatacccc
54781 acttgacaga taaggaagct gaggccaggg gaggtcaagg
      catttgtctg aggttgcaca
54841 accaggagtg gtgaagctgg gagtcacaca caaggaatca
      ctgcaaagtc tgtgtccttc
54901 ccactctgag taggacttga accagggatg tccgatgctg
      atgcttgtgt cctgaccact
54961 accttgcagc gtcctcaccc acacccaccc caggcctggg
      aggcagggga gcaccagcgg
55021 tctgggagtg aaagttgctt ccctgtggga tgtctgggag
      gtgggtagaa ggccttccta
55081 gcaggcacag ctgcccaccg gatgagtatg tccaggggga
      gaggagcccc ctgtcccagg
55141 atgtgggcaa aaacctggag aagctgaagc tggactttga
      ggatggcccc tgacYccatc
55201 ctgggacagc cacctcccct tccttctgcc ccctccagtc
      cctcctctcc ttccctgctc
55261 tccctcctac ctctttccat ctcccccttt ctgcctgtga
      ttcctcctgt gactggggca
55321 agatccttca cctctgtcct ccctgagcct cagtggctca
      cctggaacat agggtgatgc
55381 ctgcagaagt attttagggt tactttatga gataaattgg
      tagactgttt ttcccgggtc
55441 agccagccag gggttcggtg aataggagcg aacgctgctg
      ccattccctt ctcccctagt
55501 tcatcctgga ggctctccgc tctccccctg gttctaagtc
      ccctcctcct gcaccgtatc
55561 ccccctccca tccaacccag tccccactga ggcactgaga
      cagggtcttg gcccagggtc
55621 cccacccacc tgagctccgc atgtgagccg accttcaccc
      gcctgcctca gtttcccgtc
55681 cacgaaaggg gtgagcgccc ccacgtctcc tgggactgtc
      cttgggtccg atgcggggat
55741 gtggggaggt gccagggtgt gggcgcagca gggagcgggt
      gtgttaggcc cccgcccatc
55801 ccgcgcccga gccccatctg gctcgggctg gcacctcgaa
      tccacgtgat ttctcggcag
55861 cagccgccag ttccatgcac tggcggagca gctctaggcg
      gcggcttcta ctttcagttt
55921 cgtgcagagc gcggaggagc cgcgagcgct gagggtgagt
      gccgggagct ctgagggtga
55981 gtgccgggcg tgccgcgggg ctgcgggacc cgggctgggg
      cgagcggaag ggagaggatc
56041 ggggttcgaa ttctgcacgg agaggggtgg aggggatgtc
      agaggccctg gagcgggagg
56101 tgcgggtggc cgggtggctg gccgatggat agctgggtaa
      gcggacaggg cgccagtggc
56161 ccggcagcgc gcacagcatg cgccccggca gtttggagga
      agagttgccg gcccgcagag
56221 gatggcagat gagggtcgcc tggatggggg gacacagtaa
      gcggctgtgc tcccctccct
56281 gcgcagctcc tgtcacctac cggatccacc cactctctct
      gtcttttact ttgcaacttt
56341 tcattgattt taaaacaatt acggaagtta cactgttcat
      tgtaYgcaat ttagactttt
56401 tggcctaaac aactttttat tgtggaaaat tccaaacata
      tacaatagta gaaggaatag
56461 aattaggttg agccttctgc acctgcgggt tttcatataa
      tgaagcccca cctatccacg
56521 ttcagcttgg acagtcacga ccccaaggcc acctactcac
      gaccccaagg ccacctaggt
56581 agcccaatct ccaccgctgg ttattttgaa acaaatggca
      gacagcatag catctcaact
56641 gaggtatttc agaatgtact aaaagataag gcatctttta
      aaaacaaaac cacagtacca
56701 ccagcatact acaaacattt taatagttat tccttaatgt
      caaatctcca gattatttct
56761 aaggaacatt taacctcata agtgataggt gaatacgtgg
      gtgttgtttt gttacttgct
56821 ttctgcattc ccatgtggtt ttcggtcact attctcaccc
      agaaagccag gacctgaaac
56881 tggctggggt gatcattcga ggaggtcgga agaggctcct
      accttcagtg ccgtttgcgt
56941 ccattttata agttggagtt cttatgtttc cttttttaaa
      aggttgaagt ttctgcagac
57001 ctaaaaagca gtttgaaaac tggcagttcc cagcctctaa
      aacccggggg tagcaggagg
57061 cagagagtac cccggggcca gatcctggag cctcattgtc
      ccttcaaaYc accttccgtt
57121 tctctaaggg agaaataaac cttgaaaagc catgggcttt
      tcagatgtat ctagatgtat
57181 ctgatgcttt tcaggtgtat ctagactccc atccagcgtg
      ctttctgcaa aacttactga
57241 accatagccc agaatggcca tccatgatgc ccacccgcct
      tgtctcctga agcacattgc
57301 caggtggacc tgcacatggt gtggacggca gacagactgg
      ggctctcaac aactggtatt
57361 tgctggatga ccatgaacta accattcaat ttctcgaaac
      tttggtttct cgtctgtaaa
57421 atgaacatat gcaaatctat cctacaaagt gtgttttgtg
      gattgaatga gatgatacac
57481 gcccacccag tgccttgatc aaccttaaaa tgctgcacaa
      gtgtgagggg agatggatcc
57541 ttgaagaaga cagacaagca gaggggaaag atcttggaag
      agtttcacaa tatgctgaac
57601 tttattcctg gatcagaYac agagctgtcc gggtgtccac
      agggccgggt cagcctcctc
57661 agaccccaac acacataccc tcttcccctc tgaggaaaat
      tcagcaaaac ttcttttgct
57721 gttgttgttc tttattcctt tttttttttt tttttttttt
      gacgaagtct cactctgttg
57781 ctcaggctgg agtgtagtgg cacgatcttg gctcactgca
      acctccatct cctgggttca
57841 agcgattctc ctgcctcagc ctcccgagta gctgggatta
      taggcgcccg ccgctacacc
57901 cagctaattt ttgtattttt agtagagatg gggtttcact
      atgttcgcca ggctggtgtc
57961 aaactcccaa cctcagatga tccacccgcc tcggcctcct
      gtgggattat aggcgtgagc
58021 catcttgccc ggactccttt tttttttttt tttttttttt
      aatagacatg aggtctcaaa
58081 ctcctggcct caagtgatca gtctgcctct gcctcccaaa
      gtgttgagat tatgggcgtg
58141 agccaccaca cccagccata acttcttagc atgacttttc
      ccatttgtta tgagttcaat
58201 tgtgcctccc cacaaaagat atgctgaagg cctaaccctc
      ggcgcctcct aacgtgacct
58261 tatttggaaa tagggctgtt gcagatataa ctggtgaaga
      tgaggtcata ctggagtagg
58321 atgggccttg aatccaatag gaccggtgtc cttataagag
      aggagatgga cacatgaaga
58381 cagagacata cggggatcag acaatcatgg acggaggagg
      aagagagtgc ggcgatgcat
58441 ctaggagcca caggacacct agaatggcca gcagccggtg
      ggaaccagga gaggcaagga
58501 cggatcctct cctccagcct tcagagggag cccggccctg
      accacacctc gatctcagac
58561 tctgacctcc agaactgcga gagaagaaat ctcagttgtt
      ttaatgtacg cagcttgtgg
58621 cactttgtga cagcagatac agcagtactg tgacaacact
      aggaaacgaa tacaccatcc
58681 cagcatctct gctctgtttt ccatacagaa ttgaactaag
      gaccaggcaa gctaatgcaa
58741 Rtccaaacac tgtaaaaact ttggaggcaa tctaaggatg
      tgagccagat ttgtttgcca
58801 gatctttttt attttattta tttatttatt ttattttatt
      tttttttttt agtgagaggg
58861 agtctcactc tgttgcctag gctggagtgc agtgatatga
      tctcggctca cagcaacctc
58921 cacctcccag gtttaagtga ttctcctgcc tcagcttcct
      gagtagctgg gattataggc
58981 acctgccccc atgcccagct agtttttgta tttttagtac
      agacaggatt tcaccatgtt
59041 ggccaggctg gtcttgaact cctgacctca ggtgatccac
      ctgccttggc ctcccaaaat
59101 gctaggatta caggcatgag ccaccaacac ccagcccata
      tattcatatt tttaatggac
59161 ttatacatga aaatggtatc tcacttttaa aaataaatga
      tatggaatta tgcgttaaag
59221 gRaaattaaa tcttattata tcatgtttta aaatttttaa
      tttttcatag ctaatacatt
59281 tatgtagttt taaaaatgta aaacatttgg aaagatataa
      tgaaaaataa gtctccttcc
59341 atctcctgtc tcccagccac ccaattgccc tccccgaaga
      caaccaatgt tatcaggttc
59401 ttgtgtctcc ttccaaagac agtctaagtg tgtttttaca
      aacatggata tatacatata
59461 tatatacacg tatatatgtg tatatatata tacacacaca
      cacacacaca catatacaca
59521 tatatagaca cacacacaca tgtgtacttt tcccttctca
      caaatggcag tgtactatat
59581 gtagtgttct ctaacttggg tttttttttt ttaaacttag
      tgcaatttgg aaatctttct
59641 acatcaaaat tttaaaatct gccctttccc tctttctttt
      taacagctgc ctagtatttc
59701 accgtatgac tgctctgtaa ttgatctaac agttgtagaa
      cacttaggtt atttcctgtc
59761 ttttgctatt tcaacagtgc tgtaatgaat atccttggtc
      aggcatcatt tttcatacgt
59821 gggagtgaat ctttaagaaa accaggagtg gatttgcaag
      gtcaagggga atatgcagtt
59881 tgaacttgga taaataaggc aaattatccc cccttaaatg
      ttgtactaat ttttgctccc
59941 accagctgga gggggagggg ccaaatttcc atatttgcaa
      atctgggaga cgaacaatgg
60001 tgtgtttttt atgcctctta ttacgaatga gtttgaacat
      cttttcaaat atttaagagt
60061 cacctgtagc tcattttcca taaactgtca gttcatatcc
      tttgcccact tttttattgg
60121 cttttggtct ttttcctgtt gagttgtaaa agcacttttc
      atgttaaggg aatttgctct
60181 ttgtctatta tatggttata ctgtcattta aaatggtggc
      atagttgctt atagaatgtc
60241 tgaaccatat gcgtcattgt tagatattta tattgggtct
      cattttcttg tattatatat
60301 gggatgtttt ccctgttttt tgtaaaaaaa aaaaaaaaag
      caagatatga cattttaatt
60361 cttccaatag ttctcaccat aatggtgaag gaaaggaaat
      acgtttggga aaatacttcg
60421 ataggatttg gggtggctgg aacctgtgtg caaaatggcc
      aacagggacc aggaccattt
60481 tgggtgggaa gagagtgctt tgcatggtgt atgtgtatgg
      ggcggagggg ctgtatttgc
60541 tcctgaaaga aaagggggtg gagggagggg agaagaaagg
      aaggagagaa ggtggaggga
60601 ggttagccac agagggagat gactcccaga aacttcttag
      gggaaggatg gcaaaagggc
60661 attttcggtg agtccaggaa tgccctctgc tccgtgtgag
      gcaggtacca gcactgcaga
60721 caccggctct ggctcaccac ctgccatcat gagtgcattc
      agcttctcca Ytgagcttcc
60781 acacaccagg ccctgctcta ggcactgggc tacagccggg
      aacacaacca actccctgcc
60841 ttgtggagct gatgaggcag tagaaacaga caaacaagta
      aacaaagatg tgcatgtcat
60901 atgaggtcag gtggaagtga ctgcagtgaa gaaggcaagg
      caggcgaggg tggagcatgc
60961 tRgatagaga ggcagatgta gccttcttgt agggatgttg
      ggacatttga gagagaactt
61021 gaagggagcg tggcagggaa ccatgggcca tggcatctgg
      aggaagaggg aaccagtcag
61081 aggggatggc aagaaacaaa aaagtgggga gaagaagaaa
      gcagagaaga ggagaggagg
61141 gagaagagga gaaaatgctg cttaaagtct gcttttgtgc
      tcccagatgc tcattgtgaa
61201 tgacattttt gcaatttttg caattttgtt ttgtaattta
      ataatgggct gggctgggcg
61261 cagtggctca cgcctgtaat tccagcactt tgggaggctg
      agatgggcag atcacttgag
61321 gtctgcagtt tgagaccagc ctggccaaca tggagaagcc
      ccgtctctcc taaaaataca
61381 aaaatagcca ggtgtggtgg cacgtgactg taatctcagc
      tactctggag gctgaggcag
61441 gagaatcgct tgaacctgag aggtggaggt tgtagtgagt
      tgagatcgtg ccatgctctc
61501 cagcctgagt tacagaggga gactcggtct caaaaaaagc
      aacaaactgc ttgaattaaa
61561 gcaggagccc tcagctagag gagtgcccac ccacctcctc
      cccaggaatc gcagagctcc
61621 caggagaggc catagcctcc tataggtcat ggcattgcag
      gtgggttggt gtcccagctg
61681 gctgtttctg tctcaagaaa ggaagggctg ctcccgctga
      catccaggcg taagcaacgc
61741 cctggtgaac atcctcgtag ctagagcttt gctcacattc
      tggatggctt ccttaggctc
61801 aatcctcaaa gtggactcct aaagggatat atgaactttt
      attttttaaa tgtttgaaac
61861 cgtgttgtca aatttctctc ctaaaagatt gtgccaatgt
      agagaggctg ccgaccgctg
61921 gaagcttccc aatcccccag cacctatccc ttgtgggtgg
      gatggatcct gtaccatcgc
61981 cagaccctca agaacccctt gtcctgcaYg gtcccttctg
      tccctcctgg tgaaatggaa
62041 ggagcatgca tgccttggga agctggtgct ttgaggcatt
      cagatcttac cgtggcatca
62101 tgtttgaaga gagaaatagc ttgctgtttt gtgtgtctct
      caatagtctt attcactgag
62161 ttcttgaagg tgtcctgtgt gtttctggac tgcagaatcc
      ttttattgaa atggacaggt
62221 cagtgttttc ccagagaaag acctatccag taggagttca
      caagaggatt ttaggaggta
62281 tgtggagagg gcatccgatt ccattgaatc gtaaagtgac
      aaatttgttc ccttttcagt
62341 tctttttcat tcttccagac tagtgaaaga gaaagcctca
      gtcaggtgct aacgcatctg
62401 taaacctctc acactcttga tattttcagc aaggggaggc
      caggcctccg gtcagagccc
62461 tgggtctgca gcaataccca gctacagttc aacagcatca
      tagtgttttt tgttttcctt
62521 ggctttcttt ctttcttttt ttttctagac aaggtctcac
      tctgtcaccc aggctggagt
62581 gcactggttg cacagctccg tgcatagttt cctgcagcct
      ctatttccca ggctcaatcg
62641 atcctcctgc ctcagcctcc caagtagatg ggactacagg
      tgcgcaccac cacacctggc
62701 taattgtttt ttgtttttat ttttgataga gacgaggtct
      cattacgttg cccaggctga
62761 tctcaaactc ctgagctcaa gcagttttcc tgccttagcc
      tcccaaagtg ctgggattac
62821 aggtgtgagc ctccatgccc cacccttgag tttcttttcg
      atgatacctt cagttcgtgg
62881 caagtggtac ttcaaaaaat aaagttaaaa gtgacatggt
      ttctagaaaa gctttaagga
62941 aatggtgttc tggctttcag ataagtcaaa aaaaatctca
      aacatgagta aagagttttg
63001 gaaattatac acttacccct agagggtgag tttgtggggg
      aaatttcata atgtatactt
63061 ccagaatatt tagaactttc caaatgagta caggttatgt
      tcgtaaacag gaaaaataat
63121 aaagacataa taaaatgata atttagtttt aaaagacgac
      catttgttga aagcagtatt
63181 atatttcagt taggaaatgg cctctggaat cagcatagat
      tttattgttt tatttatttt
63241 gtttttgaga cagtgtctca ctctgttgcc cacactggag
      tgcagtagtg tgatcacagc
63301 ccactgcagc ctcaacttcc tgggctcagg tgatccttct
      gcctcagcct cccaaatagc
63361 tgggactata ggtgcacacc actatgccag gctaattttt
      ttgcattttt tgtaaagaca
63421 gattttcacc atgttgccct ggctggtctc gaacttccag
      gttcaagcaa tctgcctgtc
63481 tcggcctccc aaagtgttgg aattacaggt gtgagccact
      gtgcccggcc agcatagatt
63541 ttaaatccac cttttgttgc ttgacttctt ggagcctgtt
      tcttcatcta tgaaatgcag
63601 gaaacgtgcc tggaaatatt gcttgagatt ctaatgggga
      ggtgcaggtg gaatactgta
63661 gcctgatgcc cagcctatgc cgggaactca gtacataaca
      gccaatgagc attggctgcg
63721 gactggacat gtcccctcct tgtgctgtag tattaatgtc
      tccaaccctc acagtgatcc
63781 tgtgaagtaa atgtaaaatc accacccaga tgaggaaaac
      agtggcacag agaggttaag
63841 aaaactgtcc aaggttgcac agcactaaaa tgttgaaatc
      aggatttaaa cccagccctc
63901 ctgaccccag aggatctttt ttcaaaaatt atgttctttt
      attttatttc gagatggggg
63961 tctttcacag tcacccaggc tggagtgcag tcacaatatc
      acggcttact gcgcctcaac
64021 ctcccaggct caagtgatcc tcccacctct gtcccccaac
      ccccagttgc ttaggagcac
64081 aggcacacga caccatgcct ggctaatttt tgtacttttt
      gtagagatgg ggtttctcca
64141 tgttgcccag gctggtctca aactactggg ctcaagcgat
      cctcctgctt cagcctccca
64201 aagtactggg atcacaggtg tgagccacta tgcccagcat
      tagaggctct tttaacagct
64261 ccacaattcc ctgtgagatg ggctgcatac aaagcattac
      tcctgcttga catatacaca
64321 aaccatggcc caaagggagc tgggacttgt ccggatgtca
      gcctctaagt cagttttggg
64381 tcagtgagac ccaaaaccaa ggctctagcc gtctagcccc
      tccaaccttt cttctgcccc
64441 agttttatgg gtttggcaat gtctgccagg atgggaggga
      gcagttcaga tgatccagtc
64501 tggaggcaaa attctgctaa attcaaaggg cccagcctca
      agtcactgat gtcttttttt
64561 tttttttttt actttaaaaa aatgtattMa ttctaaacca
      cacaagtaag acacgaatca
64621 gtcttccttg cagaaagtta aactttttat acataaagct
      gatatctatt ttactcactg
64681 cttccagccc catgcctytc ccctctcctg cagtaacccc
      tatttgatat gtttccagaa
64741 ctttatctaa accttcacat aataaaaata attcttatat
      agggcttact aacttccaag
64801 cactgcttta agagctttac atatttaagc cccacaataa
      ctctgtgagg taggtgctgc
64861 tcttatcccc attttacaga tgagaacatt gaggcccaga
      gaggctatgt aactggcctg
64921 gcgtcaccca gcctgcagtt ggtggaaagg tcagtcccaa
      atctaggcag ccttgctccg
64981 aaattcatgc tcttagcctc taagttataa aacatgctat
      tatcgacctg tgggaagaaa
65041 cagtattgct gtttgttgat gtgtattttg agacaagtga
      tatcatgttg tgcatatcat
65101 tatacaatgt gtcttgttta ttccatgata catcttgaag
      atacagccac gtcaacaaaa
65161 aataagtccc tttcctgtac ataatcccac agcgtggtta
      caccaaactt tattatggct
65221 gtctagggtg tttaaagacc gctgctggaa tttctttatc
      caagactctg tgtgtcttag
65281 cccattgggg ctgctgtaac aaagtgccag agactggctg
      gattctaaac aatagaaatt
65341 tatttctcac agttctggag actaaagtct gagatcagag
      tgccagcatg gtcgggttct
65401 ggcaagagcc cttttccggg ttgccgatgg cggacttttc
      attgtatcct cacatggtgc
65461 aaagcaggct agagcattct cttgggtctc ttttataagg
      gcatcaatcc cattcactac
65521 ggctcctccc tcctgaccta attacctccc ccagacccca
      gctcctaata ccctcctatt
65581 aggggttaga gtttcaacat gtgaatttca ggggagcaca
      aatattcagt cgatcacatt
65641 gtgtattctg ggccccaagg ctgtccattt tgctttctgc
      tgagaagttt agtggtaagg
65701 tcaaagggta gaagcgcagt aagatctaga agtcactgct
      gatttgccct acaagatggg
65761 caaaccactt cccagcctgc agctctgagc cctggatcca
      ggccttctct cctttatgtc
65821 tcgagcttac tctgtcccct gccttactta ccaaaccaaa
      gcaagactcc tctgggacat
65881 tcctgccagg ccccatggca aggttggagc tgaggagggg
      acttccaaac cttggtttta
65941 ttttcacttt ggcttgggtg tgccaagccc tggcagagcc
      acagagttgg ggcacgaggt
66001 gccaagaaac agctcccagt ctgaggaagg tgctctgccc
      tgggaagggg ataRagctga
66061 gtgttggtta tggggagaca tcctcaaggt ctccatctaa
      atatggtcaa cttggtggcc
66121 ttcctgtccc cagggccttt ggRgctctca gaggacaaga
      atcactcagc tctgatttgc
66181 tggagaagca gagccccagg tgggaggtag cgaggagcct
      gtctctcccc actgggagtg
66241 gagagctggg tgccacagcc tcaaacagtt gttcccagac
      atcggcacat ggggaacccc
66301 ccgggagctt ccaaaagtgc cagtgcctgc gttctacctc
      ctgggatggt gctgtgatgg
66361 gtctggagtg tggcccattg ggatttttta aagaccctct
      tcccaggtga tgccgtcgtg
66421 cagacaagca tgggaaccac tggcctggaa gccccaggga
      gaggcctctg gggcccggga
66481 cacgcaagcg ggagccttct cctagcagcc ttgctagcca
      cccacccctt cctgtgggtg
66541 ctagacccaa actgccaggg ctgagcagga gaagcaaggc
      caccagctgg ccacctgctg
66601 tcccagccag gctcaggctg gcagtagggc accccagtcc
      tggaggaggg tgcatctgga
66661 aagcttgggg gctcttggtg gaaagttcaa gcctttagca
      taggaaggtg gctggggtga
66721 aggaggcagt ttaaagacta gtgatgtggg ctgggaccca
      gattgctggg tttccaatga
66781 ccagaagaag atggccttgt tggactgggg acagggacca
      cccaggctca ggcctgaggc
66841 tgggagtgca ggcatgaatt tgagtgcagg ctcaggaaca
      tccccagggc cgccaggcag
66901 ggagacacct cttcaactcc tggctcctcg actctagctg
      gtgacctagg caggtccctt
66961 tcccactgtg agcctcaatt tcctcatgtg taaagcatca
      tgactgtgtg ttttaagtcc
67021 tttagggccc tatctttgtg agggacaaca gagacgatgg
      ttttcacagg caacagtcac
67081 ccctgcatca tcactgactc acagaaatgg atatgcccta
      taaggacatc tcttgcttgc
67141 cctgccagca tttgtgggcg ggggaggaaa tggccactaa
      ccattcctga aggcacctcc
67201 caagggaccc ctgaaaggag ggtgacgcct cccagaaaga
      gaccagtggc ccagcccagc
67261 cctgctctta gttttgtttc ctctgctttg aatgccagca
      aggcctttcc ctcccagggc
67321 ctcagtttcc ccatctttac aatgggcaca gcatagaccc
      catcactggg gctctttctg
67381 ctctgccacc ctgtgcatcc aggctcaggt gttatccgaa
      gccctgacat catcagccat
67441 tcccgggggg aaggcagagg cgctgggttc tctgtgtccc
      cctggtaggg cttggtgtag
67501 gattcaggga ggattggagg gcctctgtgg cctcaggtcc
      tcccccaggg tccccaggcc
67561 ctgacctgtc cacctggacc taaagaacca acaacagcag
      cactttctgt ttctttgagg
67621 tatttctgtc caaatgtctg tagtcatgac tggggtgcct
      ggctatcacc ccacaaactg
67681 catcctttct gatctgcaca gatgagaaaa ctgaggtctt
      gtttcccctc catcccaaga
67741 cctaatggga cctcctcctt aatctaatcc tcaagaggca
      gaaacaggat ggaaagcaaa
67801 ggaatggcag ccagaagtct gYggctccac tcctgtcctc
      tctgcatccc tcacttcctt
67861 ccttacacca ggcgtcaggg gctggagaga cggaagaagc
      gatagcaacg acaaccacac
67921 tagtggtaat catagcttgt gtggattgaa cctttttttt
      atttttcatt tttaattttt
67981 gtgggtacat agtaggtgta tatatttatg ggttacatga
      gatgttttga tataggcgtc
68041 cagtgtgtaa tcacatcagg gtaaatgggg tttccatcac
      ctcaagcatt tatcctttgt
68101 gttgcaaaca attcaattat actttttgtt atttttaaat
      gtacagttaa attattactg
68161 actatagtta ccctcttgtg ctattgaata ctaggtttta
      ttcattctaa ctattttttg
68221 tacccactaa ccatccccac ctcccccacc ctggcctcct
      actatacttc ccatcctctg
68281 gtaaccatcc ttctactctc tatctccatg acttaaattg
      ttttgatttt tagatcccac
68341 aaataagtga gaacaagtga tgtgtgtctt tctgtacctg
      gcttatttca cttaacataa
68401 tggcctccag tgccatccat gtccttgcaa atgatgggat
      ctcattcttt ttgatggctg
68461 aatagtactc cattttgtat gtgtgccaca ttttctttta
      ctcttttatt cttttctttc
68521 tttcgagatg gactcttgct ctgtcaccca ggctggagtg
      cagtggcacg atcttggctc
68581 actgcaacct ctgcttccca ggttcaagca attctcctgc
      ctcagcctcc caagtagctg
68641 ggattacagg catgccccac catgccctgt taatttttgt
      acttttagta gaggaggggt
68701 tttgccatgt tagccaggtt ggtctccaat gcctgacctc
      aggtgatcca cctgcctcag
68761 cctcccacag tgctggaatt acaggcgtga gctgccgtgc
      ccagstgcca cattttctct
68821 gttcattcat ctgtcgatga acacttagat taattccaaa
      tcttggctat tgtaaacagt
68881 gctgtcataa acatagtagt gcagatatct ctttgagaca
      ctgattcctt tcttttgggt
68941 atatacctag cagtgggatt gctggatcat atggtagctc
      taaatttagt ttagtttttt
69001 tttgtttttt tttttttttt gagacagagt ctcactctgt
      ctcaaggctg gagtgcagtg
69061 cgtgatctca gttcactgca acctctgcct cctgggttca
      agcgattctc ctgcctcagc
69121 ctcccaagta gctgggacta caggcatggg gcgccactac
      gcccagctaa tttttgtatt
69181 tttagtagag acagggtttc accatgttgg cgaggatggt
      ctcaatctct tgacctcatg
69241 atctgcccac ctcggcctcc caaagtgctg ggattatagg
      tgtgagcctc cgtgcccagc
69301 cctattttta gttttttgag gtacctccga actgtcctgt
      agtggctgta ctaatttaca
69361 ttcccaccag tgtatgaggt ctcccttttc tccacataga
      ttgaggtgtt tgtttgtttg
69421 tttgtttgtt tgtttggaga tgaagtctcg ctctgtcgcc
      cagactggag tgcagtggcg
69481 tgatctcagc tcactgccgc ctccgtctct tgggttcaag
      ctattctccc acctcagcct
69541 gggattacag gcgcctgcca ccacgcctgg ctaatttttt
      ttattttaag tagagacgag
69601 gtttcctcat gttgcccagg ctgctctcaa actccggacc
      tcaggtgatc tgcctgcctc
69661 agccttccaa agtgctggga ttacaggctt gagccaccat
      gcccagcctg gattgagttt
69721 gtatcatgtg ccaggttcca tgtcagggat tccacatgcc
      ccatcccaca accatcttgc
69781 caggtgggca ttatgatgac cccccatagc agacactgtc
      agagctcccc caaccccatc
69841 tctgcccgga tgcccccact cactgtgggt gacacctgca
      tccttcttcc ggggtcgcct
69901 ttgcctggtg ggcattgcct tgctcagaga tgcctgggca
      gtcatgcctc cctcccccgc
69961 ccaatggggg tgcagtgacg tgaagtgtga ccgaccaccc
      agaccccttg cacatgcgca
70021 ggatggacct cttctatcat cacgctccag ttctcccatg
      tggcaggctg aagcRaggct
70081 tttgaaacca tatctttgct cagctgcctc tgctacccta
      tctttcttcc accaccccct
70141 caataaacaa cttccacagg aacccccttg ggctctgctt
      ctagggacct gacctagaag
70201 atcctcattt tacagatgag gacgctgagg tacgaagagg
      ctgcgtaacc taccagggtc
70261 acacagcttt ggtgtggagt agggactcaa atccagggcc
      ctggtacgga ggcatttgcc
70321 ctcaccaaga ttcaggcact gagagaagcY gacgggccta
      gagaatggca agacttgaga
70381 tagcagaagg aaggagagag ggtgagaaat ggatccccgt
      gctgaggtct ggtctggaag
70441 gagaagtctg caaggtagga aaaagaggaa aggggacgtg
      aggtagggaa gcaggatgtg
70501 caaggcgggg acttgtgaaa gagaatgccg gggggtggtg
      agcagcatct catgggcagg
70561 tcagcaggtg caggagaggc ctaggagggc cggggactgg
      atgatgaaag ggcatttgtt
70621 tatttatcct ttgtttttgt tttttgttgt ttaaattgag
      accagctctc acaatgttgc
70681 ccaggctcgt ctcgccaact gtctgcccac tcctggcctc
      aaacgatctt cctgcctcag
70741 cctccaaaag tgttgggatt acaggcataa gccaatgttt
      attcgtcctg agccttccct
70801 ggacacctga tatgtagctg ctttgtggtg gtcacagttc
      catggtgtga ctttatcctg
70861 gaggcagtgg gatgctgata gcaagttcag acaggcatga
      ggccagacca gagctgagtt
70921 ttcaagataa ctccagccag aggtatgcgt gcatgtgcac
      atgtgggtac atgtatgtgt
70981 gtgcacgtgc atgtgtgtat gagtgtggac tagagatgga
      atggctttct gagcaaaggg
71041 aacagccaag gcaaaggcct gtgggctgca gggagagaac
      aaggtttgat ctctgcactc
71101 cctcctctaa gctctgtgtc aacagcattt tcaggacagc
      aggaaggatt cccaaagagc
71161 acagcagcga gggcagttgg agggaggcgt ctgctgggac
      actgaaacca gggYggggta
71221 aactcccctc gcacctcctc agactcagag cccccataaa
      tcttccaggt gcaccccagc
71281 ccttttccct ctctctgtct gaactggggt ggcatgatcc
      caggactgca actccacgtg
71341 tctggattcc ccttactgac tgcgtgaccc tggccatttt
      ccctccacct ttctctcagc
71401 gcccccattt gattactcgg cctcaggggt gcactgggga
      ttcttagagg taacacttcg
71461 aacggaaagc cagaggtcct ggtgccaaaa tgcaaatgtg
      agggcttgaa agttcatcgg
71521 gttttatttt ctctgttccg tttgcagtag ctcttctttc
      ttgttttcta tagaacagta
71581 aaaaacttta tgcatttgaa tatcgtgaac cttgtaattc
      atatgcaata taaaaactac
71641 tagctatgca tggagaaatc aattgaagaa cagtggctta
      gcatgtcttt ctcattcttt
71701 gatggctacc tcaaatcaaa tcaatatgaa cttggggtca
      ggcatggtgg ctcacacctg
71761 taatcccaac acttggggag gccaaggcag gaggatcact
      tgagcgcagg aatttgagac
71821 tagcttgggc aacatagcgg gacctcatct taatattaaa
      aaaaaaaaaa agagaaagaa
71881 aaaaagtgaa cttgggaggt ttgaatgaca actcgtatag
      ctgaagtaat agatatattc
71941 aacttttaaa acttaatttt cattagataa cacatgcata
      tggtataaca ttctaggcca
72001 ggtgcagtgg ctcacatctg taatcccagc actttgggag
      gccaaggtgg gcagattgct
72061 tgagcccagg agttgaagtc tagcttgggc aagatggcaa
      aaccccatct ctgcaaaaaa
72121 cacaaaaaat tagctaggtg tggtggtgtg cacttgtagt
      cccagctact caggaagctg
72181 aggtcgggga atcatataag cccaggaggt ggagactgca
      gtgagccacg accacaccac
72241 tgcactccag cctgggtgac agagcaatac cctgtctcaa
      aaaaaaaagg aaaaaaaatt
72301 cctttcactg gggacaatta cctttatgaa tttcttccag
      aaatattaat gtatttgtat
72361 acacattttt ttttgagaca gggtctcact ctgtcaccca
      ggctggagtg cagggatgca
72421 atcttggctc actgcagcct ggataccccc aggctcaagc
      aatcctccca cctcaacctt
72481 gcgggtagct tggacaacat gtgtgcacca ccactcctgg
      ctaatttttg tattttttgt
72541 caagatgggg ttttgccatg ttacccaggc tcgtctcaaa
      ctcctgacct caagtgatct
72601 gcccgccttt gcccctcaaa gtgctgggat tacaggcatg
      agccaccata tttatttttt
72661 ctttacacaa aggaagcata ttctacaccc tgttttgccc
      cggctctctt ttaaaaaaca
72721 gtgtatcttg gataccattc catatcattc cataaagttc
      atcttcatcc tctttttttt
72781 tttttttttt tttttgagac agggtttcac tctgtcgccc
      aggctggggt gcagtggcac
72841 tatctcagct tacctcaacc tcccgggctc aagtgatcct
      cccacctcag cctcctgagt
72901 ggctggatgc tgcttctttc tttttgatgg ctgcaaagaa
      ttccattgta tagatgtgcc
72961 ataatttaat cataatatgt cctctttgat catttaggtt
      tttccaatct tttgctatta
73021 caagcaaggc cacgttaaat atccctgggc atattatttt
      gcacattatg aagatatctg
73081 taggctaaat acctgtgtat ggaattgctc agtgtgatta
      catggcattt accactttgg
73141 tagataaagg ttgtatcctg ttacatgtcc acggatgaat
      ttcttccaga aatattcatg
73201 tatttgtata cacatttttt gagacagggt cacaaaaccg
      ttaacacatt attattatta
73261 ttattattat tattatttca cattatttag ggaagtttca
      tttttttgtg agctatcact
73321 gataatttct tatttactca tcatcttgta tttctcttcc
      ttgcacattg gggaaatgtc
73381 tattctagtt tttaatgtaa atgttcttgt ggctttttta
      gaaaaaatca aaatgtgcac
73441 attaaaaagt ttgaatggtt caaaaggtcc taaaaagggt
      ccacagtgga aaaggctctc
73501 ctttcccagc aggcccagat ccctcctcag acacaagcct
      gtcgtcagtt tcctgtgtag
73561 cttcccagag ttggtcacta tgtatgcaaa tacacacaca
      tttaaaatat acacactcca
73621 cgtccacgtg gaatgatgca aagcatctaa gtgcccgtta
      attcacaacc ttgccaactc
73681 agtaatatcc ttgcgaatct aatagctaga aaatggtgta
      tctttttagt tctaatgtat
73741 tatatttatt ctattttctg tgagcttgaa catcttttca
      tatatttaaa agtcattggg
73801 gccaggggtg gtggctcatg cctgtaaccc cagcactttg
      ggaggccgag gcaggtagat
73861 cacctgaggt cagaagttcg agaccagcct ggtcaacatg
      gtgaaactca gtctctatta
73921 aaaatacaaa aaaaaaaaat taatcaggca tggtggctcg
      tgcctgtaat cccagctatt
73981 gggaggctga ggcagaagaa ttgcttgaac tggggaggtg
      gaagttgcag tgagtggaga
74041 tcgtgccact gtactccagc ctgggcaaca gagtgagact
      ctgtctcaaa aaaaaatttt
74101 taaatgtcat tggtgtttcc cttttttgta aaccttttat
      gttctttgcc tagttttcta
74161 tgaggtcatt ggctttttca gtgttggttt ttagtaactc
      tcgatttatt aaaacattta
74221 tacatttata gctttgttta tgatgtatac atttataacc
      ttgtctgtga tgcagatatt
74281 tttccaattt gtcatctggc ttttgacctt atctgtggta
      ttctttgaca atacaaaaat
74341 tttaatttat atctgatcag tttcatccag ttgttttttt
      cttttttcat gatttctgag
74401 ttttgtgtca tgcttagcaa gatgctctct actctgaaat
      tattttctgt aaaatatttt
74461 tttcagccag gcatagtggc tcacacctgc aatcccagaa
      ctttgggagg tagaggtgga
74521 aggattattt gagtccagga gttcaaggcc agcctgggca
      acagagcaag accccgcctc
74581 tattttttaa aagagaaatt tttttaaaat actttttccc
      atgttttgtt tgagcactta
74641 cattttctta acttttttgt cgtgaaacat aacacataga
      aaaatgtaat gtaagtgtac
74701 aacttcatga attgttataa attgaacata tagctttctg
      gtactttcat ggtttaactt
74761 cttacattta aattttggtt tcatctagac tttattttga
      tataattgtg aaatagaaat
74821 ccatttttat tctaaaagaa cactttttaa atgttcatga
      aatgttttaa aatttattat
74881 ataccaagca agaaaaaatc tcaacatagt cctcaaagca
      gaaattgcac aggacttatt
74941 ctttgtacag tgtaatgcac aaaaactaat aagaaagcct
      taaaaacaac cctttattaa
75001 attactccat tccaaattat aatttcaatc tacttagcac
      ataagtattg atatacattt
75061 atcgaaattg gccaaaagtg ttttattatt ttttaaaaga
      aaaagcctag caatggctta
75121 agcaataaag tttttaattt taaagaataa caaacagcct
      cactaaatca gaggaaagaa
75181 ataaaaagat aaaagcaaaa attacagaac tagaaaaggg
      accattccag caaaattgat
75241 aaatgaaaac aaaaatttgt ttttcaaaca taagagttac
      caacggctgg tgaatctaaa
75301 aagaagaaaa aacaaagtaa actaaattaa atgtgaagcc
      atgaagagat ttaaaattat
75361 aagaaaatag gccgtgcacg gtggctcacg cctgtaatcc
      cagcactttg ggaggccgag
75421 gtgggtggat catgaggtca ggagcttgag acccgcctgg
      ccaacatggt gaaaccccgt
75481 ctctaccaaa aatacaaaaa ttagctggct gtggtagcat
      gagcctgtag tcccagctac
75541 ttaggaggct gaggcgggag aatcacttga acccaagagg
      tggaggttgc agtgagccga
75601 gaccttgcca ttgctctcca gcctgggtgg cagagtgaga
      ttccgtctca aaaaataaat
75661 aaataaataa agctgggcgc agtggctcat gcctctaatc
      acagcacttt gggaggccga
75721 ggtgggcgga tcacaaggtt aggagatcga gaccatcctg
      gctgacatgg tgaaaccccg
75781 tctctactaa aaatacaaaa aatgagctgg gcatggtggc
      gtgtgcctgt ggtcccagct
75841 actcgggagg ctgaggaaag agaatggcgt gaacctggga
      ggtggaggtt gcagtgagct
75901 gagatcacgc cactgcactc cagcctgggc gacagagtga
      gactccattt caaaaaaaaa
75961 aatacaagaa catattatat acaactgtga gctacaccat
      gtttgaacat cttgacaaaa
76021 tgagcaactt tctcaaaact aaattgccaa atgttcattt
      ttaaaaagcc aaaaataacc
76081 aaccaacaca atgaggataa atttaaagtg atcaagtttg
      atttttttaa aaaactgttc
76141 aaacatcttg gtagtgctgg gcaggaagtt ggctttcatt
      ctgggtgaga tgagaaggct
76201 ctaaggggca taggacctga tgtcatggga cctgatgcag
      gctctgtctt aatagctgac
76261 tcctgcgtgg agtaggttat tgggggatgg gtggacagag
      accagggagg aggctggtgc
76321 aggaatccca gtgagaaatg atggtgtcca ggctaggcca
      tagcagtaga ggaagcaaga
76381 ttctggaatg cacttggaaa atgaagccat ctttgggctt
      gagctcaagg aagcatgaag
76441 ctgcccctaa ctgaagtggg gaaggtggtc tggggagcag
      tttaggagga gagatcagga
76501 gctcagtttc ggacttggaa attttgaaac gcacatgagg
      catccaggtg gagttgggag
76561 aattaaataa tgctgaaagg tactcacttg gcaagtagca
      aatattcgct atattgtctg
76621 ttcaactttg tcaactgctt ttccagtaga aatgtagtgt
      ccttatcaca catgcataaa
76681 tgttaaactc aaaagtaaaa tgaaatattt tctcagtaga
      tatgaataac tttttaattt
76741 ttttgttttt tgacatgcgg tctcactctg ttgcccaggc
      tggagtgggg tggcgagatc
76801 atagctcact gcagcctcaa actcctgggc tcaagcaatc
      ctctgccaca gcctcccgat
76861 tagctgggac tacaggcaca caccaccacg cccagctaat
      cttttatttt ttgtagagac
76921 tgagttgcac aatgtagccc aagctggtct ccaactcctg
      agctcaagct atcctcccac
76981 ctcggcctcc caaagtgttg ggattacagg cgtgagccac
      tgcacctggc tgctttttaa
77041 ttgtaatatt catcctccat ccatcatact tagtgcaaat
      atttttcccc acctattgcc
77101 ttcctatttc tcttttattt cacgtcattt agggaagttt
      catttttttg tgagctatca
77161 ctgataattt cttatttact catcatcttg tatttctctt
      ccttacacat tggggaaatg
77221 tctattctag tttttaatgt aaatgttctt gtggcttttt
      ttaaaaaaaa atcaaaatgt
77281 gcacattaaa aagtttgaat ggttcaaagg gtcctaaaaa
      gggtccacag tgggaaaggc
77341 tctcctgtcc cagcaggccc agacccctcc tcagacacaa
      gcctgtcatc agtttcctgt
77401 gtagcttccc agagttggtc tctatgtatg caaatacaca
      cacatttaaa atatacacat
77461 gccacagctt tttaaaattt gaatttatgg gccatgtgca
      gtggctcacg cctgtaaccc
77521 caacactttg ggaggccgag gcaggtggat cacctgaggt
      caggagttcg agaccaacct
77581 gaccaatatg gtgaaacccc gtctctacta aaaatacaaa
      aattagctgg gcatggtggt
77641 gggtgcctat aatcccagct tctcaggagg ttgagacagg
      agaatcgctt ggacccagga
77701 ggcagaggtt gcagtgagct gagatcgtgc cactacactc
      aagtctaggc gacagaggga
77761 gactccgtct caaaaaaaaa aaaaaaaatt gaatttagac
      tcaggtgcag aggctcacgc
77821 ctgtaatccc agcacttacg gaggctgatg aggggagaat
      cacttgagtc cagtagttca
77881 agaccagcct gggcaacaca gggaaactat gtctctacaa
      aaaaattaaa attagcctgg
77941 catggtggag tgtgcctgtt gtctcaccta ctcaggaggg
      tgaggcaggg gaatcacttg
78001 aactcaggag taggaggctg catgagctat gattgcacca
      ctgcactcca gcctgggtga
78061 cagagcaaga ccctgtgtca aaaaaaaaaa aaaagaaaag
      aaaattagac taccatcata
78121 gatggtacaa acacttccca gtttgttgct ttcgttttgt
      ttgttgccat ttttactata
78181 cagaaaaaaa aggcaataaa gaatacatta ttataatatg
      ttaatttcta ctgcattatt
78241 tttaacaact tcattgaggt gtaatttaca taccgtaaaa
      tctatccatt ttaggtgtag
78301 gattcagtga tttctggtaa atttactgag ttatgtgacc
      atcaccacaa tctggttttg
78361 ggacattttc attactgcca taatattcct caaacccatt
      tagaatcatt cctatttctg
78421 ctcccaggcc caggcaacca gtaatctact tgctgtttca
      atagatttgc cttttctgga
78481 tgttttacat aaatagaatc atacaatacg tgatctttag
      tgatcttttt tcacatggca
78541 taaacttggt tcctttttat gttgaataat attccattgt
      atagatatgt cacagtttgt
78601 ttctccattc atgagctttt gggtatttgg gttgatttca
      ctttttgact gttatggata
78661 atgctggtac aaacattcat atacaagttt ttgtgtagat
      gtatattttc atttctcttg
78721 gaatagacct aggcgtggaa ttgctggatt agatggtaat
      tctattgttt tacattctaa
78781 ggcaggggtc cccaaccccc aggctacaga tcagtgtcgg
      ttcatggcct gttaggaact
78841 gggccgcaca gcaggtggtg agcggccatc gggcgagtat
      tactgcctga gctctgcctc
78901 ctgtcggatc agcggtgcca ttagattctc ataggagcac
      aaacccagtt gtgaaatgcg
78961 catgtgaggg atctaggttg cgagtttctg atgagaatct
      aatgataaat gtaatgagct
79021 tgaatcatcc cgaatccatc ccccaacccc aacccccgtc
      cggggaaaaa atgatctctc
79081 atgaaatcag tccctaggcc aaaaagtctg gggactgctg
      ttctaaggaa ctgccaaaac
79141 tgccaaactg tttttcagag tggctggacc attttacatt
      cccaccggca atatatacgg
79201 tgtcctgttt ctttacatcc tcatcaacat gtgtcttttt
      cattacagcc attctatgtg
79261 ggtgtgaaat gctatgtggc tgtgtttttc atttccatgc
      agaaattttt aatttaattg
79321 cttagttaaa aataattctt taaatttaaa acactcatat
      tgccatatgt atatttttat
79381 actacaaagt gatacaattt ttgttaattt tcttgccttg
      attactcttt acctgtatag
79441 tatttaaatc tttacataag atcacaatta tagtgaatag
      taatttattc aactcaaaat
79501 cttcatagga tttagaaaat ttaagtcctt taccaaaggg
      attgacttYt aacttcatta
79561 aaggttgcga acagtatcaa atccttggta aagccacatt
      caatctttgg cactccagga
79621 ttttgagcta taactgtagt gatcactaat atgtcatttc
      taagctgacg gtcataatga
79681 ctaaaacctc tcatagattt taaaatactt ccttcaaagt
      cagtaaacat tccaagttac
79741 taagctgttg aacaatttct tattttgaag attttttctg
      ataagttcca aagtgtttct
79801 gatgaaggaa ataaaagctg tccagagaaa tctgagtcat
      tgaggtgagc acagattcca
79861 ttggctgctt gtgctttcat cattacagta caattaactt
      cagaagtcga gagatgctgc
79921 agaattttaa gtacccaaag tttctcaaca agttgatttt
      caagcaaggt cattaactgg
79981 gccattgttt ttgccaatcc tccaacttca gccatttgaa
      tcttgtatga tgaactcact
80041 tgctggtaac ctggaatatg ctcctttggt ggttgtgcct
      gataaaagtc aacaatacgt
80101 ttacaatttt ttatcctcaa ttcagaactt ggtattttca
      ttaagtcacc cagaagcgca
80161 actgaactag cagtatctcc agcataagtt atttcatccg
      atactttctt tttcaaaaat
80221 ggcaagccac actattttat gatatcatat gcagattcta
      caaaatgcgg ctggttttca
80281 atttttactg cacacagatt cagaatttca aatgtctgta
      ctaaatccct taagggaagt
80341 tcattctgat aacactgtac cagtttcttg acagatttaa
      gttgcttttc ttctaagcct
80401 tctttagcag tctcttcaaa aagtttgatg acaccattaa
      ggtccacagg cttcaaaaca
80461 ccagttccac atggcgaggg aggccttggg aaacatcatc
      atgatggaag gcaaagggga
80521 agtaaagacc ttcttcatgg tggcaggaaa gagacagcat
      attgctatgt attttaacac
80581 cacttatttc cccccttttt tctttcaata tcatggatta
      aatagtgtat tttaaatgag
80641 tgtgaggctc acttggactc tattccatcc ctttggccaa
      tttttttcca tttttaatcc
80701 aagaatctta aagtttgaat gatsgtcacc ttgttatata
      ttttattatg tttggaagct
80761 tttttgctat tgtctgatcc tcccaacaaa tagccttttg
      atagaattgc ctttattcgt
80821 ttctttatat ctaattctaa cttaagatta gacttaaaag
      taacttcagc ggttttatta
80881 tgaaatattt aaggcatata aatactgcct cttttctcta
      aatgactgct aatataattt
80941 taccaagttc tgttaaagta tcctgttgta cataaagtgc
      tgttgttttt tatctgcctg
81001 ttaacttaga gtgggtttta ttttctttca ctttctgtgg
      tcgaaggcaa gtatgtctta
81061 gtcagtttgg gctgttataa tgaaacactg tagactgggt
      ggcttaaaca acagaaactt
81121 gtttctcacg ttctgaaagc cgggaagtcc ccaatcaggg
      agccagcagg cctggtgtct
81181 ggagaggatg cgcttcctgg tttgcagacg gcaccttctc
      attgtgtcct cacagggcag
81241 agagcaaaaa gaaagggcaa gcgctctcca gcctcttcct
      ttttcctttt tttttttgtt
81301 tgtttgagat ggagtcttgc tctgttgccc agactggagt
      gcggtggcat gatcttggct
81361 caatgcaacc tctgcctcct ggcttcaagt gattctcctg
      cctcagcctc ctgagtagct
81421 gggactacag gtgcctgcca ccatgcctga ctaattttta
      tgtttttatt agagatgggg
81481 tttcatcatg ttggccagac tggttttaga ctcctgacct
      caagtgatct acctaccttg
81541 gcctcccaaa gtgctgggat tacaggcgtg aaccaccgca
      cccggcctcc agcctcttct
81601 tgtgagggca gtaatcccat cataaggggt ccaccctctt
      gacctaatca cttcgcaaag
81661 gtcccacttc caaataccat cacactgggt atttaggctt
      caccaggtga attggtgggg
81721 gaggtgggac acaaacattc agtccacggc aagtttttat
      ctcaatttgc taaagcctct
81781 ctttatttct cctgttacag tgtgataatg ttttttaaaa
      tatcatttag ctggctgtgg
81841 tgacccatgc ctgcagtccc agctactcag gaggctaagg
      caaaaggatc acatgaacct
81901 aggagtttga atccagcctg ggcaacatag aaagaccctg
      tctcttaaaa aaataaataa
81961 ataaataaat aaataaataa ataaataaat aaataaagga
      aagaggctgg gcacagtggc
82021 tcatgcctat aatcccagca ctttgggagg ccgaggcggg
      cagatcacct gaagtcggga
82081 gttcaagacc agcctgacca acatggagaa accccttctc
      tactaaaaat acaaaattag
82141 ccgggtgtag tggcacatgc ctgtaatccc actacttggg
      aggctgaggc aagagaattg
82201 cttgaaccag aaggtggagg ttgtggtgag ccgagatcat
      gccattgcac tccagcctgg
82261 gcaacaagaa Igaaactcca tctcaaaaaa aaaaaaaaaa
      aaaaaaagaa agaaaagaaa
82321 agaagcactt aaagaggctg aggcaggcag atcacttgaa
      gtcaggagtt caagaccagc
82381 ctggccaaca tggtgaaacc tagtctctac taaaaatact
      agaattagcc agacgtggtg
82441 gcacatgcct gtaatcccag ctactcggga ggctgaggca
      tgagacttgc ttgaacctag
82501 gaggtggagg ttgcagtgag ctgagaacat gccattgcac
      tccagcctgg gcaacagagc
82561 aagactccat ctcaagaaaa aagaaagaga gagaaagaga
      aatatagaca tatgttatta
82621 gtcccccagg acctcttcaa aagatctctc ttttcctttc
      ttatttctaa ttaccatact
82681 acataccaca tccattatgt cagaatgtgc cctatttacg
      tggatttgtc taaagtcatg
82741 gaactctgag ttacattccY cgtatttcac agagacacaa
      tcagagcaca cgctgatctt
82801 ccgcctctct tcatttcctc catgtactct gtagctacct
      gttctcttct ttgttcgtag
82861 gaagatctct gttgcgtggt gtgggagttt gtgccagctc
      atctttttat tgtgctttct
82921 gtgagcagct atcaatgcct ccaggtcttc tctttaagaa
      gaatgtttac tctcaattgt
82981 ttaaaaaact aactaaatag gaagaagatt gcaaatccta
      acctttttgg ggttttatat
83041 tgggggcaaa agttggcatc tgcagtaagg attatttcat
      tttcatctga ataaatgagc
83101 agtaataact ttgctgactt gcagggctca ttgtgatgcc
      aaaggacatt gtaaggtgca
83161 tatctgaatt ggaggaggaa gaggggcatc ctctgagcca
      ggtgtggcag gaggtgacat
83221 caaggagggt taggctgcgt aaacccaggg acacaaggag
      ctgcagtcgg ggaagaacaa
83281 ggtctagcgg atggagttgg ggggactgtg tctggagcca
      atgttctcag atgtgtctcc
83341 ggcaggcagg ggcaagggct ggtgcctggt ttacagcatt
      ccttttggag tctggaatag
83401 tggggaaaac tccctgtctt ctctcccctt aggagtctgc
      actatggaaa caacctgtca
83461 atccagctca aggcacacat agcccagaca cccatgagac
      cctctccgtg gggaccctag
83521 agcacctatc atgaacgagg agaccaagta ggtgattttt
      ctctcccctt cacctcctct
83581 tttcccctcc tatctacttc gtcacttttc tcctactgcc
      aagactggtc cctacccaaa
83641 tctgccttac tgggctgccc ccaacacaac aaacctgccc
      ttgaccatca ttcagcatga
83701 gaatgtaatg atcagacaca gaaaatcagg aagagttttg
      agacagcatc taatctaccc
83761 attttagaga tggtatgact gaggcttata taggaaaaag
      gcttgctcaa ggtcacacta
83821 tgagtgtatc agctaggata ggccaggtta cactgtagta
      catgcaccct ccaaagtccc
83881 agtgactcat ggcaacaaaa gtttatttcc tgctcttgct
      tcctgtctcg tgtggattga
83941 ctgggattgc tcctccacat catcctgtct cccacactca
      ggctaacagg gtagccatga
84001 tttgcaatat ttctggtccc tggagtagaa aaaaagaaaa
      agtactgtca attacacctt
84061 ggttcttaaa acaactacag gcatgacatg agtctttttt
      agcatgtttc attgaccaaa
84121 gcaagtttca tggctgtgcc caatttcaag gaggcagaga
      agtgtaatcc caccaaaggc
84181 ctagaaagag gagaaagagt atctgtgaac agccctaatg
      cctaccacag agaattagta
84241 acagagctgg gccatggatc tttcaaccat cgtcctctgt
      ctccaagatt attccccatg
84301 gaaatctcaa tgactgataa tgatgccatg tgctttttct
      cctgaggatg gttagcctta
84361 aagagatttg tgtcatatcc taaggggata gaacactaat
      gatggaaaca aaggtattgt
84421 gttagactgg ctggaggaga aatttgagga tgagaaacat
      caccagaaat cacaccatcc
84481 agycactact tacttccatc tggtgccatg ctgccctgga
      ccatttcttc atgggtagca
84541 tggatgctgg acaccatagc ctggaaaaga agttatgccc
      taggctatgg ccacatcctt
84601 ttgccgagat cacacaaaat ccagggactt agccttgtct
      ggtgccatct aggcctccaa
84661 ggccttggaa atttactggc ctgggaggca agaaacctca
      gttctcctcc tgcctctgtc
84721 atsgacttgc tgtgtgactg tgggcacacc ctttgccctt
      ccttggccat ggtctttccg
84781 tctgcttagt ggaggtggtg atccctgcct gaggatcatg
      gtgtgagcat gtttcataaa
84841 ttctaagact ctctgcctga gtgaggacct attgctatta
      gcccagttga ggagaaatga
84901 ccagtggggc tgacagtaca tccaagtatg tctctactgc
      atgcccaggg agcagctggg
84961 ctctaaaccc caggacgcat agtactggcc tcagcctctg
      ggaattggga aggctggctt
85021 ctgggtggac ctcctactct tttcagagtt agaggtggac
      tctctggagt tgtgaggata
85081 aaataaggta tataaagcac ttagacagtg cctaatacac
      agtgagtgct ttccaagtgt
85141 gtactgctat gaggaaggac atagtaccag aaagtacatc
      cggaaatgtg atagcatagt
85201 ccactagatg atggaccacc atttagttgg ccaatcccca
      actgttggac atttaggttg
85261 tttccaattt ttaataatta taaataatct tgcagtgagc
      atctttgcaa ttaaatattg
85321 tatatacgtt catgatcgtt tgccaaggtt agagtcctag
      aaatggaatt actgaatcaa
85381 aggaactgca cattatttag gcttttgctg tgtaaatgcc
      ccgtgccctc tattggtact
85441 tctagaagga ggtggtttaa gagttccagt tcggccatca
      tgatggctca cacctgtaat
85501 cccagcactt tgggaggccg aggtgggcag atcaccctga
      ggtcaggagt ttgagcccag
85561 cctggccaac atggtgaaac cccatctgta ctaaaaatta
      caaaaattag ctgggcattg
85621 tggtgtgcac ctgtaatccc agctactcgg gaggctgagg
      cagaagaatc gcttgaaccc
85681 gggaggcgga ggctgcagtg agccgagatt gcaccattgc
      actccagcct gggtgacaga
85741 gcgagactct gtctcaaaaa aaaaagagtt ccagttcaga
      agggaagaat ttgcttagca
85801 aaagaggtga tggtctgtgt tagctaggat tagagttcac
      tgtgagtagc agaaagcact
85861 aaacaacagt ggcttaacca gaatggaagt gtatttccct
      gccatgtaac agaagtccaa
85921 agtcagtgag cacagggcag atgggcagct ttaccttcca
      ccttcctcag tatgggttcc
85981 acctcatgat tcaatatggc tgcccaagat ccagccatcc
      catcaacaat ccagacaaca
86041 ggaaggagca aagaagaata gacaacattt ctacttacat
      tccactggcc aaattacatg
86101 gctacatata actgcaaggg agactgggat ataataatct
      attctagatg accagagaat
86161 atttagtagt tctaatactg aggaagaagg gaagaacaga
      ttttgggaga caactagttg
86221 tctctgccat gaggttagct cagactgaga gcagcataaa
      tggttcatgg aaataagaat
86281 gagtaatgca tcctcacggg actgtccaca ggggatgaac
      aagaccattc cagagagcca
86341 gggtgcaggc acaaatggag tagggagggg gtgcagaaga
      aacagactga tctctggagc
86401 tgatccctgg gcagagacat caagactcca tggagaggcc
      acttccctgc ctattttatg
86461 tcKctcaaat gtagggactg gttcttgact gcttgacttc
      cctagaaact cccttatccc
86521 ccaactcacc ttScccagat caccttcccc ctagttcatc
      tgtaccccac tcacctgccc
86581 tcaggtcacc tgccccacaa ctcacctgtt ctccatctaa
      tcttcctccc agctcccctg
86641 tcccccaaat cacctgcccc caatttgctt gtcctccagc
      tcaccggttc cccagctcat
86701 cttccttcca gttcccctgt cccccagctc acctgcctgc
      cctcagttca cctgtccccc
86761 agttcatctt cctcctagct cccctgtccc ctagctcacc
      tgtgccccag ctcccctgtc
86821 ccccagttcc cctgtccccc agctcacctc cccctcagct
      ctcctgtccc ccagctcatc
86881 tgcccccagt ttacctgtcc cctagctcac ctccccccca
      gtttacctgt cccccagctc
86941 acctcccccc agtttgcctg tcccccagct caactccccc
      caagttcccc tgtccctcag
87001 ctcatctgcc cccagtttRc ctgtccccct caactcacct
      atcttccctg tccctccagg
87061 gctggctcct catggacccc gttggcctcc agctcggcaa
      caagaacctg tggagctgtc
87121 ttgtgaggct gctcaccaaa gacccagaat ggctgaacgc
      caagatgaag ttcttcctcc
87181 ccaacacgga cctggattcc aggaacgaga ccttggaccc
      tgaacagaga gtcatcctgc
87241 aactcaacaa gctgcatgtc cagggttcgg acacctggca
      gtctttcatt cattgtgtgt
87301 gcatgcagct ggaggtgcct ctggacctgg aggtgctgct
      gctgagtact tttggctatg
87361 atgatggtaa gggcaggtgt gagagacgca aagcagccgg
      gccagtacct gcgtcatggg
87421 gagccgggag gagcccaggg acccacctaa gtcagagaga
      gggtgtagcc cagccacgat
87481 ctcaactcta tatttctgga cacttcccag aacaaatttc
      ctaatgcgcc caaatctgct
87541 ggggcctgga ggacccctcc ttctacctgg cttcactcga
      attgctcgaa ttgctgagtg
87601 actgcttcca gccacaaaat cccacatagc ccataatgcc
      cgagagattt atttcttctt
87661 ttttggcggg ggtggggggg tgttttttgt ttgttgttgc
      tcttgttggg ttttgtttgt
87721 ttgtttttta tttttgagac aaggtcttgc tctgttgcct
      aggctggagt gcagtggcac
87781 aatcatagct cactacagca tccaactcca ggactcaggt
      gatccttccc acttcagcct
87841 cccaagtagc tgagactaca gtagaactat aggtgcatgc
      caccacatta ggctaatttt
87901 tcattctttt gtagagacag gacatcactg tgttgcccag
      gctggtcttg aactcctggc
87961 cttaagtgat cctcctccct tggcctccca aagtgctggg
      attatagatg tgagccatca
88021 cacctggcta gaggtgtttt tgtttgtttg tttgttttgc
      aacatggtca gaaacagtga
88081 cggggagcgc cttctgccct ctactgatcc gtggggattt
      cttgggcacc ttctgtttgc
88141 acagcactga ggggaaacac aggacattga ggggcaacag
      cgtttttgga gagaaaatgg
88201 gaactcactt aagaacccta cttcaaggca gagtgtggga
      attgcggggg gcggggcggc
88261 gcatgcacat atgtgcagac atatgcagat acacacagtg
      catatacatg tgggtatgtg
88321 cgtaaaccca tgtacacatg catgcaagtg tgcccacatg
      cataacacat gcatgcctgt
88381 gcacacacat acataaactt gcatgcactt gcatatacac
      acatttctgc ccagcactgc
88441 cttcaaagct caccaccaca ttttctccac aacagataac
      tgagtgttcc atttagttct
88501 cccttgctgg gtatctccct gtgagatgag ccctgagcca
      ggcgccaggc cagagctggt
88561 ccccagcatc cctcgacagc cccataccac attatactct
      cccctctctt gcagggttca
88621 ccagccagct gggagctgag gggaaaagcc aacctgaatc
      tcagctccac catggtgagg
88681 actggagttg gggggtggga agggggtggt gagcactgtg
      aagtccccac ttccaagctg
88741 gaggaggctg tggaggctgt ggtggctggt cacagccatt
      tctcataggc catgctcttc
88801 gtctgtccct gacccatttg caagtgtgtc aagcccctaa
      ctgtgtctcc cagccccacc
88861 tcctcctgca gtgactttca aggctttagc ttgtcccgtt
      accaattctM aagggtctaa
88921 cctggaaccc ctcaggaagc ctgttctcac tttaYtgccc
      gcaacccgca gggcctcatc
88981 tcccagcccc aaagtgtcag gcttcatggg gaagccctaa
      Ycaccccctc ccgacttgta
89041 caccttggac ttgacRctca cccacagctg ctgtcttcag
      cgggggagcc tttggctgat
89101 tttctgaagg agctcggaag ggccagtgat taaataatga
      ccacctactg attcgtgggt
89161 cacactttca tctcagcacc ttgatttcta cagaagcatt
      gactttactt atcgctgtgc
89221 atggcagtaa tgccaacaga aaacccaccc tataacctca
      gctccccagt gaatcaaaac
89281 tgtccaagtc cctggtccaa taggcccgca tcactgagct
      ggcatcatta tctcatggct
89341 gtaaccagat ctgattgata tttggccagt gagtcccttt
      ttaatatagg atttgttaag
89401 atattgaagt gctctcgaac tggttccagc ctcctagctg
      gacaaggagc ccagggccct
89461 gctccagggg ctgttcctat tggtcactta gcttagctgt
      aggaggaccc tgcttgactt
89521 cttaaaattc tttcctgagt cctctttcct ttctccctcc
      cttcctttct gggtcccctt
89581 ccttcttgtt ttccttcttc ctttctcaaa ctcttccaga
      aaggcattaa agtgattgtt
89641 gaaaatccat acaatgagac aagactccag aaactattaa
      gggaagtgag gaaatgtggg
89701 tgataagaaa tcgggatgat aaaaccctaa cagaggaggg
      aggttgcagg cctggccaaa
89761 ctcactggga acttcactta tatgaccacc ggcggcttcc
      atagagcttg gtccttccct
89821 gatggacaga tggccattta gtgcttggag ctgagtgttt
      ctRggggctg ctggctgtYg
89881 gccctgcttc ctgcatcctg gactgcaaga ctgccaggcc
      cagaacatcc accttccaat
89941 gggggtgcct agaagtccac tctgcaaata gaagctggtg
      ttgacccagc ctctgcctgc
90001 tgcctgcctt tccagtccct gcatgtgggt gtacttggct
      gcctgccacc tgtctgtgga
90061 ctggaKttcc ccaaaggttc tgggtaaccc ctcagcccag
      Rtccccagac cccactcctc
90121 caccccttcc tttgcttttt caggcctgaa gcgcccacat
      cagagctgtg ggtcctcacc
90181 ccgccggaag cagtgcaaga agcagcagct aggtgggtac
      cagtgtgggg aggaacataa
90241 acagagagat ggggttgcct gggggccaag acccggaccc
      tggaccttgt cccctgccaa
90301 taagcctcca gaggcccagt gactcttgtc tcgcaagttt
      ccaggagaaa aggactggag
90361 gtcttgcaca ggcagaggag aggcctgggc tggcagcgaa
      ggggctcccc acccatctgg
90421 ctgcatgggg acttctctga agagggctcc gctgaaggag
      ctggaagcac catacaggtc
90481 tcagcttcgc tgtccaggct tttccaggga caccctatgt
      cctcagtgtt gaaggagacc
90541 cagggaggag accagtttgg gggtgagcag ggatggggag
      gggtgaggag gcctgggaga
90601 aaccttcaag acagaggccc tgggggccca tggggagaca
      tggagctgct tagggcaggc
90661 caggccagtg agagaagaaa ggggacttct ctcattcttg
      accagttctc agaatggtcc
90721 tcgttctcca cgagttgtcc cactaactag agccttctct
      gcccccactg ctgccaacca
90781 tggctataac cagaggattc acatccgctc aaaatagttc
      actaccttgg cctttcccca
90841 tagggttccc ccaaaagcag ccccagccct ttccctgggt
      cacctgagac ctctcaatga
90901 cccaggaatt aaaaggttaa catctggcac tgtaggggcc
      cagctcccac agttccgatc
90961 ggtaatgcct aggcctacag gttgttagtg ctcacccctg
      ggcattgcag aactgaaata
91021 ttgggaagRg gcactgggga tccagatatt acagaaagag
      gttgtttcaa taagatattg
91081 caaagtagct gggcgcggtg gctcacacct gtaatcccag
      cactttggag ggctgaggca
91141 ggtggatcag ttgagcccag gaattcaata ctagcctggc
      caacgtgaca aaaccttgtc
91201 tctactaaaa atacaaaaat tagctgggtg tggtggtgtg
      cacctgtagt cccagctact
91261 caggaggctg aggcaggagg atcacttgag accaggagtt
      tgcggctaca gtaagctagg
91321 atcgtgccac tgcactggag cctgggcaag actgagactc
      tgtctctggg aaaaaagaaa
91381 aaaaagagat gttgcaaagg aggtgcccta ttcccctccc
      cccatcttac tgaMccctta
91441 caccacgtgt actattgcag aatggataga accgtgaccc
      acacttcccc attcccacct
91501 gcctgtttaa atagctaccg gagagcctat gctcccttag
      aactgattcc ttctggaagt
91561 tggggtggca cagatccccc tatggtgctc ttgacacccc
      caccctcacc ccatcataca
91621 tacacatctg gggctacagg ctcccaacag cccaatactg
      gggcaagaag acatgagcag
91681 agggccgggg ggtcctctcc tcatgccatg tccctgcccc
      ttgcagagtt ggccaagaag
91741 tacctgcagc tcctgcggac ctctgcccag cagcgctaca
      ggagccaaat ccctgggtca
91801 gggcagcccc acgccttcca ccaggtctat gtccctccaa
      tcctgcgccg ggccacagca
91861 tccttagaca ctccggaggg ggccattatg ggggacgtca
      aggtggaaga tggtgctgac
91921 gtgagcatct cggacctctt caacaccagg gttaacaagg
      gcccgagggt gaccgtgctt
91981 ttggggaagg ctggcatggg caagaccacg ctggcccacc
      ggctctgcca gaagtgggca
92041 gagggccatc tgaactgttt ccaggccctg ttcctttttg
      aattccgcca gctcaacttg
92101 atcacgaggt tcctgacacc gtccgagctc ctttttgatc
      tgtacctgag ccctgaatcg
92161 gaccacgaca ctgtcttcca gtacctggag aagaacgctg
      accaagtcct gctgatcttt
92221 gatgggctag atgaggccct ccagcctatg ggtcctgatg
      gcccaggccc agtcctcacc
92281 cttttctccc atctctgcaa tgggaccctc ctgcctggct
      gccgggtgat ggctacctcc
92341 cgtccaggga agctgcctgc ctgcctgcct gcagaggcag
      ccatggtcca catgttgggc
92401 tttgatgggc cacgggtgga agaatatgtg aatcacttct
      tcagcgccca gccatcgcgg
92461 gagggggccc tggtggagtt acagacaaat ggacgtctcc
      gaagcctgtg tgcggtgccc
92521 gcactgtgcc aagtcgcctg tctctgcctc caccatctgc
      ttcctgacca cgccccaggc
92581 cagtctgtgg ccctcctgcc caacatgact cagctctata
      tgcagatggt gctcgccctc
92641 agcccccctg ggcacttgcc cacctcgtcc ctactggacc
      tgggggaggt ggccctgagg
92701 ggcctggaga cagggaaggt tatcttctat gcaaaagata
      ttgctccacc cttgatagct
92761 tttggggcca ctcacagcct gctgacttcc ttctgcgtct
      gcacaggccc tgggcaccag
92821 cagacaggct atgctttcac ccacctcagc ctgcaggagt
      ttcttgctgc cctgcacctg
92881 atggccagcc ccaaggtgaa caaagacaca cttacccagt
      atgttaccct ccattcccgc
92941 tgggtacagc ggaccaaagc tagactgggc ctctcagacc
      acctccccac cttcctggcg
93001 ggcctggcat cctgcacctg ccgccccttc cttagccacc
      tggcgcaggg caatgaggac
93061 tgtgtgggtg ccaagcaggc tgctgtagtg caggtgttga
      agaagttggc cacccgcaag
93121 ctcacagggc caaaggttgt agagctgtgt cactgtgtgg
      atgagacaca ggagcctgag
93181 ctgyccagtc tcaccgcaca aagcctcccc tatcaactgc
      ccttccacaa tttcccactg
93241 acctgcaccg acctggccac cctgaccaac atcctagagc
      acayggaggc ccccatccac
93301 ctggattttg atggctgtcc cctggagccc cactgccctg
      aggctctggt aggctgtggg
93361 cagatagaga atctcaggtg agtaagagtg gaggaggacc
      ggggagggga ttgggctttt
93421 gggggagcag aggccaacca gtctagcaaa cctctgccaa
      gaggactgga taagaatctt
93481 gaatatcaga acataatggc ctgcaatgca agagaggaag
      ctccatacct tgacaggacc
93541 tctggagtcc aaatggatcc tcctcttgtc tgtggtttct
      ggactctcag tctctctgtc
93601 aatcatgggc tgaaaccaca gatccaggct gtctaYagag
      gctggtggac tttgggcaag
93661 tatatggagc acacacatgc acactgtcta ggccagagtt
      tcttcgttcc taaaacgaat
93721 ctgtaacaat agccaactca tatggtcatt gcaagaatga
      gatgggatgg tgcatgaaaa
93781 acattttgta gcacagtgcc tagcacagag caaaggctca
      gtgtgcatat tagctagaga
93841 aaaggctagg ctgcagtaac agagaaacct cctccccaaa
      tacagtaggt taaataatgt
93901 agaattttat ttctctctca tggaagagtc agaggtaggt
      ggtccaggtc agtggaatgg
93961 ctcagctcca cgcagtcatg caggtaccca ggtccctccc
      attttgctcc attatcccct
94021 tgggaatcct atctgcacag tcaaagctac gtcatgggta
      tatctgtgct ctggcttggg
94081 aacaaggaaa gagcatggag gaacatgtaa atgcattaaa
      gttcagtcct gaaactagga
94141 tacatccctt ccactcacat tccattggtg agaacatagt
      cacctggcca cactgagctg
94201 caagggaggc tgggaaatgt gatctctggc tgggaaacca
      tgttcctcat gatagctcta
94261 ttgctgtaga tgaagtggag aatgggcttt ggtggacgat
      tagaagtctc ggccataata
94321 tgtatttgtt agtactactg tattaagccc catctctctg
      aggggatggc gatgacttct
94381 cagctctgcc cagcactgat cctctgacac ttcgcttctt
      cttatggcag ctttaagagc
94441 aggaagtgtg gggatgcctt tgcagaagcc ctctccagga
      gcttgccgac aatggggagg
94501 ctgcagatgc tggggtgagc caggccttgg agctgagaag
      ggtcttcagc tggggatgcc
94561 tggtactctc ctccctctcc ttaatcctac acaaggcaga
      ttcctggccc cgcccttttc
94621 ccctcctcgt tcctccccac catctttgct tactctgtag
      gttagcagga agtaaaatca
94681 ctgcccgagg catcagccac ctggtgaaag ctttgcctct
      ctgtccacag ctgaaagaag
94741 tcaggtgagt gatctccagg agggctcact gactggggag
      atgagccact gcccaggtcc
94801 cagagtcccc ctggggcctg catgttcctc caagtctgac
      cagtagtttt tcagtgagac
94861 ccagagaggg aaggcatcag tccaagtact cagtagggct
      gagtccaaac ccaaggctcc
94921 cagagtcccc agagcaaggc ttccttcatc taatatttta
      gactgtcttg gaggagcatg
94981 agtgttaagg ctccctccct ctttctgaaa tcccaggaga
      agggatagca gtgcgtaggc
95041 aggaatcagg cactgtgggt acacataact acatgaagca
      ccacacacct tcacatttgt
95101 tcacacatgt ctacacttgc acagaagtac acaggcatat
      gcaagaatat gcacccacac
95161 atgcccactc ataggtgtgc acatgtatgc agcgcacaca
      tgcaccttta actacatgta
95221 cacattttga tgccagcaca ctctaactaa ctaagcttgt
      atgcacaaat atactcctgc
95281 cctgtgtctg ggactcagta ccatggggag gagtgggacc
      agtgaccctc ctccctggca
95341 gaacgctgct agtttatatg gcacctttat gacatttaga
      gaaggcacca cttcatccac
95401 acagatgcag cctgtcacta aggttcagca cttgttgagt
      gaaaataata agttggacca
95461 cagtccctat tgtcgtctcg gctgggcatc tgtgaaccgt
      gattaagcca aggttcagaa
95521 gtactgaact tgttgacatt tttcttccct ggctattgaa
      agtgacttta attttgtctt
95581 tcatcaccca ccattgttct taatcaccct cactcccctc
      aactcatctg tctccccagc
95641 taggaaccca ggcaaggctg gcttgtgaac aggtggggaa
      agtgaggtca gagaggaatg
95701 cgcttggctc agggctctgg gtgagtcagg gccacagccg
      aggctggaag cgatgtctgc
95761 agcgtcccag tctagacttt actcattgcc tggggggcct
      atcccaggca catcccaggc
95821 ataagccctg ggagcagggt gctaggtctc ctcattgtgc
      ctcatgaaga ggccgtcagt
95881 ctgtcttcta gatcccttga gccgccaggc aaaaacttcc
      ccataggaac agaatagacc
95941 cagaattgag gccagcagag ggtctttcct cctagccgtg
      cctgctggaa tccggcgagc
96001 aggccatcag ctctttctgg gcccctgtct tatgtctccc
      acatgagggt ggccatcctg
96061 tcacttgcta actggggctt gcaagtgccc caaccccagg
      gcaaagggac tgggccttgg
96121 tctcctcgca gttttcggga caaccagctc agtgaccagg
      tggtgctgaa cattgtggag
96181 gttctccctc acctaccacg gctccggaag cttgagtaag
      tgatctttcc actgcctctg
96241 cagcccagtc cctctctata cctgattcac cccactccct
      tgcaaggcaa ggaaggtctg
96301 gggcccctgg ggtaggggat tccactcctg acacctttgc
      cacaatcttg cagcctgagc
96361 agcaacagca tctgcgtgtc aaccctactc tgcttggcaa
      gggtggcagt cacgtgtcct
96421 accgtcagga tgcttcaggc caggtgagca gaaggaaagg
      gatcttggcc ttatgggcct
96481 tgagaaaaga ggaggggaaa gtgggatggg aaatggagga
      ggcccctcgt ctgcaggata
96541 aatctgtgca taccctactg ttttactcac agctttacct
      tcagaaccta gctcaggctt
96601 ggcacctggt acttggtata tattgaatta atggatggat
      gaaaagatgg atgtatggat
96661 ggatggctga aaagatggat gggtggatga aaagatggat
      ggatgaatgg atggctgaaa
96721 agatggatgg gtggatgaaa agatggatgg atggatggat
      ggatggatgg ctgaaaagat
96781 ggatgggtgg atgaaaagat ggatggatgg atggatggat
      ggatgaaaag atggatgggt
96841 ggatgaaaag atggatggat ggatggatgg atggaaagat
      tgatgggtgg atgaaaagat
96901 gaatggatgg atggatagat gaatggatgg atggatagat
      ggctagctga aaagatggat
96961 aggtgtgtga aaagatggat ggatggatgg atggatggat
      ggatggatgg atggatggat
97021 ggatgaagga atgagtcaac aagtctacga agcacaggca
      tagccaaagg aataattagc
97081 tgtgtgttgc cctccatggt gctgaaccaa tatagccagt
      tttgatcatg gggtaggtta
97141 ggtgtcattt ttcttactca taaaaatttc tattaaaaaa
      aattcagccg ggctcagtgg
97201 ctcatgcctg taattccaac actttggaag gccaaggcaa
      gaggattact tgagctcagg
97261 agttcgagac caacctgggc aacatagtct ctacaaaaaa
      tgaacaaaat taatggggca
97321 tggtggtgca tgcctgtggt tccagctatt tgggaggctg
      aggtgggaag attgcttgag
97381 ccgtggaggt tgaggctgca gtgaggtgtg aacacaccac
      tgcactccag cctgagtagc
97441 agagtgagat ctccatctca aatttaaaaa gaaaggaaaa
      aaaaaaactc acaacattct
97501 taatttttat tatatttctt gtgaaaattg tttttctgac
      tttttaaaac actcttgcag
97561 gattcatcaa gcaaagacat aatcagtatt tcacatgcct
      gatttaaaac acttttgcta
97621 gttccgttat gtttccctgt caattgcaaa atcagcttta
      tctggatgtt tgaacatttg
97681 gggcagaaaa gggtgtgcct gtggtgggtg atgtgctggt
      ttccagtctc tgggtttcat
97741 ggcttggtat ctgatcctgc agggaggcgg acctcatctt
      ccttctttcc ccgcccacag
97801 agacaactgc agagctacaa aggtaagaag ccaagaggcg
      gtgggcctgg ggccatcctt
97861 agaagcaact tgggctcagg cagttgaggg gaaaggtgac
      caagagacta ggaagagctg
97921 gggtatggga attcttcctg ctactgctgc actgaggaat
      gaattactgc ttaggttggc
97981 aaaggatttt aacttcctct agYgtgtcca gaccacatct
      ggtccctgtg gaaggaagca
98041 tccccagaag actcagagga aggcaaaccc ttggggagaa
      cataaagata ctgttagact
98101 ggcattacta gatggtgaaa actctggaaa agcccttaca
      actgaaaaaa gacagggagg
98161 gcaattatgg tacctacatc ttagggttgt tctgagaatt
      aaatgagtta ctacacttaa
98221 ggagtttaga gcactgttgg catgcagtgg

following is a genomic nucleotide sequence of a PROL4 region (SEQ ID NO: 2).

>12:10841901-10975900
1      tgaggagctt gaccagccct ttaaaccaac tcagcaagtc tgaatgatgt gcaaagggac
61     aattagatat gggtaagaag tccccaacct caacccataa aggactgtgt ctgaggttct
121    tacagcatca agggagatgg ataccatggc ttccatcaag gtgctgctag tcctgctctc
181    aaaggctttg gtgaccctga gctctgctca gagcatagat gatggcaaRa ggaggaaatg
241    gggacaatac aatggataac ggggttaaaa tgcatggaaa gtactagagt tagattgacc
301    ctttcagaaa ttaagcactt tattttcaga gagtaggtca ataaaagagg aggaaacctt
361    aatttctRtt tttcactgat tgcagatact tctctgaaaa tctcggccta agtcttaagt
421    caacgtcttc atgatgtaat tttgccgttc taatgtatat ggattttcag ttgttgattc
481    tgtttgtgtg aatcagggca tctaaattga acggaaaatc tatatttttc atttctataa
541    tctataatag aattctataa ttctataatt catctagata tcRcatttcc ttgacatcaa
601    actcttgaac atttaaaaat attgataatt ttgattaaat ttgtacttaa tcaaaatttt
661    gatgctaagc aggcactatt aaattctttc tattccattc cttaatcctt taaaatacag
721    gtcacccctc attcagagtc aatgattggg actagggttc tactttggga aactttggtt
781    tattgtatcc aaatctttat aatttttttt ccctaaagct ctcttaggca tttttaaatc
841    tcttgtgtct aaacctaagt gtctttctag aaattcacag ctggggtttt atcaaatcat
901    gttttaaatt tgtcttcttt ttcatttttt tttttctctt aaaatcctaa gtactgttgc
961    tgaagagttc tttcaagagt aaggtaaacc tttattcagg gatgtgttct tttctttgtt
1021   cttcagaagc taccatagtt ttcttttttg tttaataaac attctcaaaa ttctcaaaca
1081   ttctcaaaat tgagtttagg gagtcccaaa gggaaaaaga gaattacaaa atctgaaagt
1141   tttagtggtt ctcaataata acattgattg attgagacaa gcctcctctg tcacccagat
1201   tggagtgcag tggcatgaac acagctcact gcagcctcca cctcctgggc tcaagctatc
1261   ctcctacctc accctcctaa gtagctggga ctataggcac acaccatcac acccagctaa
1321   ttttaaaatt ttttatagag atggagtctc accatgttgc ccaggcttgt cttgaactcc
1381   tgggctgaag atatcctccc acctcgtcct cctgaagtac tgggactaca ggcatgagcc
1441   accatacttg gcccaataat gttaaatatg gaccatctcc ttcattttca cattagaatc
1501   tcttgtgtca gagttgtaat tagcatttga acatcagcag aatatagaca cagtacttat
1561   ttgtaaatgc ttttctctaa atctttctac aaccaatggc taatgattga ataacacaaa
1621   tctcccattt ttgcaagagc aataactccc ttgacatata actaatttga agcataaaga
1681   tacataaaat ttgcctacat tattgtagtc aatcagtgaa tgtatcattg cactttcttt
1741   tgccactctg aattaaaaca caaatgcttc attctttagt tttcaagtcc tgataactgc
1801   ttaaacacta atttgtcaca gagtaagtga aatttaaagc caccattagt cacacacagt
1861   ttacatgaaa aatttgaagt ccattctaat ggcctgggga gagactctgt gtaggaggaa
1921   ggatataata catgcccaga gtacatgaga gagctaatga attatgccac caaagaagtg
1981   gcagaattga gtaagtgagt caaccaacaa ttttgttgtt caaagaaaac ttttatgctt
2041   aatgattttt ttcccctgtt tctgacttgt caatgaccct tctactgagt ttatgatgtc
2101   atttcaaact tcattctttt tctcttcttt ctttgaagga ggctttcaag aggaagatga
2161   aaagacctaa ctaccatgtg agctattttg agttcaatta agttattaag aattgttcta
2221   aatggattca acgaacctct gggttactgt tctaataaaa catttctaca agtttttact
2281   gtaatacaaa taatcatgtt taaccatgaa aacttttttt ctctctttat ttacaaatat
2341   tttctttttt gtacacaaag ttcctggcta ctctaggctg gttagtacag ggttgatcac
2401   cctaaaatat ctttgccaca ataYgaatga tgtgcttttg atggtatgtc ccagatacat
2461   atttcctttt gtggcctgac atatggcatt gtctggagaa tattcaatat atgcttgaaa
2521   agagaatgtg ttgtctcttc cttttatgga atctttcata aatatcagat caggttgctt
2581   gatagtgttt gttaatgtat tctatattct tacagatgtt ctgttgttca acaaattact
2641   gaaatagaat cattgaaccc tttaaaaaaa gatgtcttat atacaatcca aacaatattt
2701   ctctgggatg tagagatgat ctacattgag gcaatatatc ctttgagagt tttgacattt
2761   tagcataacc atgtattagc ttttgattga atgtcttgtc acagcatctt gataatcatt
2821   gcctggtgca gtccgtggac atacgtcttt tgtggagaaa ttttcttcaa taaataatta
2881   atagttcaat ttaggcttct tattttatag acattttcta cataattttt ctcactttta
2941   cttatagatt gtccttcatt cattgatctg caattctcaa ataatctttg aacttactgt
3001   gacaatatat ttagccataa tatatacctt ttctgtgatt ataagattat tcatagaatt
3061   ccacagcatt tattaagaac tgtcacttta ttgagtcttt cccaaaagta gagctttgtt
3121   ggatgatcca agaggaacca tctaacgcgg actatgagaa ggaactagtt gcttttcata
3181   aagatgcaaa taacagcaag atgctgttac agttgttttg aattagtcac acaactttgc
3241   atctggaaac acggtttaga actatgaaaa tgccctctac tcttgatagt tgatattagg
3301   atattaattt ctatttgctc tgaaacatgt tttaaaaaac agcaactcta aagaaaggaa
3361   gagttacaga agagagaaac agggatacac agacagagtc agaaacagag acagagataa
3421   agagatccag ccagctgtac ataagattcc ctacattaca attacagata cactaattaa
3481   gttttaggag taattaatca cataaaacat taatgcaaga actgaagtta atttagaaac
3541   atcttatctt tgtttgtcct agaaaagcat agtttctctt cagatttgtt tatgttgttg
3601   gaattttctt ccttttagaa tagaYattac tttccaaatc acctttagag atgcatgtct
3661   taatttattg ttccccagta ttaggataaa tgaatgactt gaggggtaKa ttagagctat
3721   ggactcacca aaaatcacag ctaattccgW ctctggcata aagtagctgg aggtggcaat
3781   gagaaaggac aaatagtagg caataaagag gagaaggaag gaaatgacag ctttcagggc
3841   tctcacatgg gcttctgtgc tggggtctct gcaccctgtg gcactgagct gcattcgcct
3901   gatatgtctc cgcagggaga ggatcaagag gaaaaaggac attaggcaca cacaaaaggg
3961   gagcagcgtt gccaggttga gaaataactt ggtagaagca tgttgagttt tatttactct
4021   gcaactccaa gttaagtttg ttttcctctt tgccttcaca caaaacctga aatcagcgtt
4081   caaattctca gtggctggaa ggctaataaa cacagagaga accacgcacc ccagtagaat
4141   ccaggaaatc accctgtcaa ttctccactt catccagagg aaaagtgggt gaaagaaatt
4201   acctatcttg aagaaatagt aaatgctgag gcaggttgca aaccagatac ttaaatgatt
4261   ggttagtgtc cagaagaagt caatgattct catttcttta ccagtggcat agacatctgg
4321   atatagcacc aatataaaac aatctaatag tattacgcac aatagacaaa ttctggatat
4381   ggccagactt gtgaggatta aatcaatgga ggcaattttc ctcttcttga cccagtccat
4441   gcagtttacc aatccaatga atgcattccc taagatcccc actgaaaact ctccaactgc
4501   taagaacaat aaagtagtct gcactttatc tgccatgttt aaatatgcaa ttagtttcta
4561   gttgacctga tggagtttga catccacacc tgcttcttag attttgatgt agttttcttt
4621   acctctttgt tgtagtctgt tttgtgatgg agactggaat gataaatgaa gactggagct
4681   atcttcatta aatcctaatt agtcttgaca aacaatgtgt ttgScgatcc ttggcctggc
4741   cactgtatgc ccatttacct actcagcagg ttgtaatttt Ycacatacgg ttaatttagg
4801   ctgagctact gtttactaag atgcaaattg ggccaattcc ctttcatggt cctgcattgt
4861   ctttctgagg ctaaggtgta cgcattttgg attcattaat ttgtgatgta tgctagtaac
4921   ctcaataggg ttgctagatt tagcgactaa aaattcagga cactcagtta aacttgaatt
4981   acaggtaaaa aaatcaataa ttttttagtg tatgtccctg attacttaag atcagtgctt
5041   tcaggaagta ctgaagttca aattagattg agtgttccat tctggcaacc tgaacatcaa
5101   gaatttggta gcaagtataa aactggtaaa attggaaagt tcttgaagaa tttttatttt
5161   gctgaatgtt aatattgata attagtaaag aatccaaagt ttggttccat agtgagagtt
5221   atatattttc aggaacttta aaaagttaac actaaatgta ggttatttga ttccaaagaa
5281   actttaggtc attattttgg ctgttcagat gaacaattac atggcatagt gtctcttaat
5341   acaacacatt gtcttactga ctaaatgact tttaatggtt atttagcaat gttatatata
5401   ataaggtaga ggacctgtaa taaaaaaaga ctattgatta ccttaaattt ttcattttca
5461   cttaaaatgt tctcaaggaa actttgagag gtattagata tgtccattac cttgtttgtg
5521   gtgaagatat catgggtgtt tgaaatttta tacattaaat atatgcggtt ctttttatac
5581   caattatact ttcataaaga tttcaaaaat ttaaaaaatg ttctcaaggg tagttgtatg
5641   catgagagag gagatagtaa aattctgtat ttcattctct ccactgtcct ccatacaaat
5701   tgcatgagta tctagatcct caacccttct agcaggagac tgaagattaa actttccaca
5761   tctctagaga tgaattttta gtttgaaata gcttctctaa tttagagtta ctgagaagcg
5821   ctttactaca tggggttcca tagaatagtt cttgggtatt cacaccttcc tgggaatgca
5881   tgtcttctct tatccttcct taaaggacaa accatcattc cggagatgta gccttaactt
5941   ttggacaatc tgtatgatta tgtagccatg ttcacaggca actggaacat cacctatgat
6001   aggacgatct tggatatgat ccctgaccta gacttatcat ttcttttgtt ttttttttga
6061   gatggaattt cgttcttgtt gctcaggctg gagtgctatt ttggctcacc gcaacctcca
6121   tctcccaggt tcaagtgatt ctcctgcctg agcctgccaa Rtagctggga ttacaggcag
6181   gtgcaaccat gcctggctaa ttttgtattt ttagtagatt ccgggtttct ccatgttggt
6241   caggctggtc ttgaactccc gacctcaggc gatccgtgtg ccttggcctc ccaaagttct
6301   gggattacag gtgtgagcca ctgtgcctgg cctagactca tcatttttac cctacagttt
6361   atcctggtaa atgaagtgct catgggaact atgatgatgt attttctcaa ataattttta
6421   gacttgtttt tgtttagttt tggaaataaa tgtagatttt atgaatacca atgttgtata
6481   gcgtgaagtt atgtgaatat atagttaaaa aaaaagttct atgagagagc attttgtcaa
6541   aagcttacta ctttgaaccc cagtcactgt ggtcactaga gatgcttgaa acattagaga
6601   ggcaggaaac aaaatatgga agaaaaaaac ttaaccctaa tggggtaaaa ccttcccaaa
6661   agtactaatt caataaaacg tttatttcac ttaaacctta ggaaaaatat aaagttcatc
6721   ctagtataaa atctacctgt attttgtaga atacattaat atatttcatc aagagaacat
6781   gaattcttgt gtcttgtaaa acaaagagta acatatacag ctagcatatt agaaatatag
6841   ttcataaaat ttatatactt ggggctataa atattagtac tttacagagt gagttttaca
6901   taataataag acaaacaact gataattttc cggtaattag tattttagtt ataataaaag
6961   gaaattagta gattgaaata catcagaatt aaacagaaag taatgggagt aaaagcaata
7021   atgtacagcc atattagtac atacacaacc tgagatatgc cagaccaaac gtttcaataa
7081   tattatagtg aggatgKatt tcatgttcac agaaaaatta aaaattttag tgacttattt
7141   tccttttttt tttaataaaa atttatctgt attttttgta aacattcgtt tcttcttcca
7201   gggacagtat gtgcttattc catggtatac gctattttct tcccaattta tttcttttgt
7261   taagtcctga gtgaaaacct ttgcagggtc tggttagaga aaagaacctg attgagcagt
7321   ctgactaata tttgttatcc ccattctttt atccagattg tgaagagggc ttaggaacac
7381   cccttggtgg gtggcacttg aatgggttcg attcccacct aggtgctgca tgaaaagcaa
7441   gttgtttgag aggaaattta attccaagga aaatcaatag aaatcatcag aataaggtca
7501   accagaaaat attacctagg agaccatgga aatcatcatt cgggaaaacc aaagacagac
7561   cagagaacat ctgagttcct aatcaataag ccaaatgggg gtgactcagc agagccatga
7621   aaggagcaca attaaggagc ggcaataaaa atgtatgaca aagaaaaatg agaaaccagt
7681   tactgtagct caggtccact ttaccatgag ccaaagtaaa atctaaaaag actttaacta
7741   ggcaatacag gcctaacact tgcgttgagg agacaagaag agaagcatga gctctgaaat
7801   tcttcgagga aagggagtgt aaagatgagc atttctgagt tgtaaaggga tagaaaagat
7861   gtattcagta gtttgctact aggtcctaat aatatttaaa gatatgcatc caaaataaat
7921   gagataactg gggaaaaatg agaatagact attaagaaaa ctatattttt tgttagagaa
7981   gatttggttt taaattccat ttatacagct aagatttcat atcaYgcagg caatttttct
8041   acatgtcagc attctgacaa atgtctgcct cagtttatta tttaaaacaa ttaaaataag
8101   tgagtgaccc aaggggtaga gaattgctgc aatctctcca aactccacag ctaacttgta
8161   ttttgtcata agatagctaa aggtcatcaa aatagaagaa atatagtata ggaaaaaaaa
8221   gaagataaat gaagtcatag ttttaatggc tctcacatga acttctgtgc tggggtctct
8281   actgccggta gcatagagtt ttatttgctt ggtatgtctc cataaagatc ttactaaaag
8341   gaaaaatgat atcagtgaca caataaatgg gacaattgca aacaggttaa agagagtYaa
8401   gggttcaaag tatggtattt tactcacatg gaacatttca gtaatgtttc ttttatgttt
8461   ggcaattgca tgaaacctat aatcacaact cagtactatt gctgctataa ggctgaccaa
8521   caaggaaatg gcaaagcatc ccagcaggat ccagtgcacc accatatcaa ttttccactt
8581   cagccagaga aaaagtggat gagaggaact ggctatcttc agaaaataga agacattaag
8641   gcaggtggta atccacatat ttaagtagtt ggcaaatgtc cagaaggtaa aaatgactat
8701   ctgttgttta ttttttgtat aaacatctgg gttcagtact attacaatgc catttacaac
8761   cattacactg atcaaacaaa ttctggcgat aactaaattg gtaaggatgt agtcaactgt
8821   ggaaatcttt ttcttcttaa tccagtcaat ccagttgact agtgcaatgt atccattccc
8881   caatattcct agtatgaatt ctccagttat taggattata aagatgttat ctgcaggact
8941   gaacatgttt gtagagagaa caatctgatt tcaaatatca ctgtagatga gctaatttac
9001   aggtgacctg ttaaagcatg atagatcaat tcttcgaatc atgcaataat gttttcatct
9061   gctgttattt cgacgttgtt attcttccgg aagtgttcag ctctcctctg aaataggatt
9121   gtctctacac tcctgaagat gaaaccaact aatgagcagc ttgactagtt atttatagcc
9181   tggaaaaatt ataattatta tcctttagtg agtgatacct gaaatgtttg gctgaaattg
9241   ctcccatgca aattcaaaga gattttatat catgaatttg caacaggctt cttgctgtag
9301   ctctatattt ggatcttcaa attgagtttt gaatagggag tcataaaaaa agagataaat
9361   gtttaataaa aacccaactg tgtaaataaa acttcatttt catcatgtaa gttataaata
9421   agccacaaat cagttctccc agatgcaaag aaattttcac tttggaagta attagtacaa
9481   ttagattgta gatatttaac atttagtcag aggataataa ttcttgtctt catttaaacg
9541   gctaattagg gaaggatata atacaaatct aattagaaat gacttttttt taggcaggca
9601   catgataata tttactaaga atttgctttc ttttcaattg ttcctcagaa gtgaaatatg
9661   ccaggtaaag atttgttatc tttagttttt ttttaatgtg acaaataccc ttgaagacaa
9721   ttcatttcct gaatcactgt ctcttgtata ataagcattg taatctaaaa ttaagaaaat
9781   ttaaatttta cattattcta gttccaatca actcctttta taagatttgt aatctcacag
9841   tgcatattat agtcatattt ctagataggg caaacacata aaaaagtaac tacttgggag
9901   actgaggcag ggggatcatt tgaaccggga ggcagaggtt gcagtgagcc aagatcgtgc
9961   cactgcactc caacctgggc gatacagtga gactctgtct caaaataata ataataattt
10021  ttttttttta cataccagta tttccaagga ataggactaa ttctactatt aaattagatt
10081  tatttgtttt ggggtaaggg aaagtacaac ttagcccaaa atacatttga atgtaggtaa
10141  tacRaatcac aataccctag ggcttcttgt atttaaatag ttctacaatg tatacaacac
10201  atgcaggatt ttttctattt cttacatgtg tgtaatgtgt aacaggaaag cataaactta
10261  ataacttaaa gaaacctagt tatctataaa ctactgctgg aaacaaaata gaaaagaaaa
10321  tgatgcagag ggaaaaaaat atgtcagaga tttctgagtt agaaggctag agagaacatt
10381  ccaRggaatc tttgggaaat gacttctaaa attgccatca ccagctagag tactcatgaa
10441  cacgctgact actgggaccc tcaaccttct ttctcaaggt ggttctgcta tacataactg
10501  tgcctcctct ctctgtcaaa ctttcattct tgagattata ctccttttcc cacatcatat
10561  atcatcttag atagaatctc attaaagatt gtttttctcc cttcttgatc aagacaaacc
10621  cttccagaaa tggagaggaa agggcggtga agataaggac cggccttcat ttcaaagtag
10681  agagttgttt tgcatgattg attttatagc aagggttgat gagttctaaa atcttttccc
10741  caaagcctgt ttgcctgaca ttcttctact cattgagttc aaggcctttg ccagtatttg
10801  tcagaaaaga agtagaacct ctttgtattt agtcaatgac ataaaatgat gaaatgttag
10861  tgtgggattt agatgcattt tttttttctg caacgacaaa gaatatacgg tgcaatgata
10921  acattagatt atccattaaa caagacaaaa gtgtgaagga acacaaaaca aatcttcaga
10981  atcatgacag atattaatga cttccaatag catataagag aaaatgaatg caaacaagag
11041  aagggagaat gccttttgtt gatttctttc atccttgtag tcaggatact ctttaggggt
11101  ctctatggaa caaaaRgctt tcttcttcta aRgaaacact tcacaaatct taacatcttc
11161  agaaaagctt ccctcaactt gctatttccc ataattagaa tgaatgaatg gcttgatggg
11221  aaaatgacag ttactatgtc accaatcatc aacactaatt ttccctgagg aatcagagcg
11281  ctagaggtca taacaagaaa gactgggtag tacacgatga ggaggagcag aaagatgatc
11341  actgccttta tggccctcat gtgggcctct gtactggggt ctctgaaccc tgtagcatgc
11401  agtcgaatct gcttggtgtg tctaactagg gagaaaagta acaagaaaaa tgagatcagg
11461  caaaggataa agggaaccat cRcccccagg ttcagggtta actgtttgaa agtacctgga
11521  attttactca ctttgaattt ccaagtaatg ttttcttcat gactgacttt gaaaaggtga
11581  taccacatat catcattctt tggaacacta ataattaaag agataagaaa ggaccccaga
11641  agaatcgcaa gcatgacctt gttgatcttt agcttcagcc agaagaaaaa tgggtgcgat
11701  atattggcta tcttgagtaa atagaagata ctgaggcaag aagtaaacca gagacttgaa
11761  ttattggcaa atgtccagac aacattcaca atgcttacta gcacgctatt gccatatgta
11821  cctggaaaga gcagcataaa gaagccatct aatgatatta cacacagcag acagattctg
11881  gagatggcca agctgatcag gatgatgtca atcaaggaaa tatctcttct tttgagccag
11941  tcaatgcagt taactagtac aatgaatcca tttccccaaa tccctatggt caattcacca
12001  gcaattaaaa taatatatat tgcctctatt gcacttggca tgccagcaaa gacttgtgat
12061  ttttgaatat cactgatgat gaattgactt tcagctgacc agtgaagaac aatgtatctg
12121  tctgcctctt agaccatgat atagttgtct ttatctctcc tttttgttag ctgtgatgca
12181  gatgaagaag atagccagtc ctgctctggg ctagagctgc ctaaagactt caatgattgg
12241  ggccagtaat ttcctttggt gcagtgatga ctgaaagcct ctaggatgca aatgggaatg
12301  gatctgattt cttaaatttg cacactcttt cttattttaa gctctgtata ttttggattt
12361  atcaatctga gctgttgacc aggaacttca agaaggtatc aattacttgg aaaatatggc
12421  tagaatttaa tttgctggat gactatccat ccttgttagt atattgacat agttattgct
12481  attacagatt ctaggtttac tgtgttagct tgtgtggcca gtttgcttgc ttggttgact
12541  aaagctttaa acaaattatt attaacatta aatgaggctt aaatggcata actggtggaa
12601  tgtagaggaa agaatctaaa ggtgtaggaa gataggagta ttaaggtgaa tttatcatat
12661  gcgaacttca ccttctcccc attatatgac ttaagagggc caaaaggaca ctccattcac
12721  tagggcattg agaaatacat taattaggta attcccaata tcctcaaaaa gctctgcagt
12781  gtttgtcttc tcaactgaag atacaatgag ggaaataaac atgacattgt agtgttgtca
12841  gcaaatgaga cacggaaaat tctggtttag atgttagggt ttcttagagt gttcttgctt
12901  tgtacctgaa tgtagcttcc atacatagaa aaaatagtca aaatactggt cacttatctt
12961  tagaatggaa acagatcagc ctgaacagtg aggaggacaa agagcaagaa agtgatattt
13021  ttggaactaa aaagcgtgat agatatacaa gactcttcag tctgaactga gtgataagaa
13081  tacatgctct gaattttctt agctctattt tttggcagca ggtgtattat aaatattagt
13141  atgctaatta tacacacatt ggaaacacac tgtttcattg ggacatattg aaacatgtca
13201  ttgggcatag tagaacatat ttgaaataat agaaaaaggc acacagcaat acacagttcc
13261  cagaaatatt attttgaaac taatattaaa ttttaaggca atattaaata ttgcttatca
13321  aacaaacaca acaatacaaa gtatttaatg agataatgtg tcatatgttc agggttttag
13381  acaaaataaa gagataaaaa ttaatcttgc agatttttat gagaaagtaa atagctattg
13441  ttattctatt cagtgaaatt tggggtttct ttcatgcttc tctgtgaatt ttgtttctga
13501  agaaatatca gattatacaa ctcaaaaata cacactgtgc ttgtcatgac caaagccata
13561  aaacaggtaa tattctgaga aggacttcac tagagtaaag aaattgattt gcatgcaact
13621  gacagacaag gtcgcaccat gttcacatct cagggaatgc aatatttcat tattttaatt
13681  aaactttttg aaagataaaa ttttgtggag gggaataaag taggttcaag gttattattt
13741  ggaaatggaa ctactctggg atcaatttgt aatgtccaga aaagatgaag atgaaaatat
13801  atcatgtagg agggtgatta ataactgaca tattaaaaat tcctggtaaa tccagatata
13861  gaatccaagc ttgccttctg aatcatggtc tttcccacta cctcacttct tttccttagc
13921  cgtgtggaat actgatcaat ttaagaaagt tatgcctata ttcaaatgta aacgaaaata
13981  tgctatagga tatgaccaga gttacctaag ggcctatact tactaaaatg ccaccctctt
14041  ttatctttcc ttgctataaa taaaacccaa atgatctcaa tttatttgac accgttgaat
14101  atgttttttt tgtgtgtatt gtgtgtgtgt gtgagtttat gtgttatttg ccctttggat
14161  tttatcttag cattgagaag ttaaagaggg atcctagctg acagacttta acatctattt
14221  tcattgcaga aacactttaa tctagaatgt tagtttattg attatttctg tctctactag
14281  aaaacagatg aaatactggg aaacttatga catcatttcc tgtatgtctt tctatgagaa
14341  gtgagcaaac acaaacctac ttaggcagat atttcttaaa tttcattttc tttcctttgt
14401  ttaaatactt tttaccattc tttagaacct tacatctttt acaggcattt gtccttgagt
14461  tatgccactg attattactg atcaagagaa atgactatac tcttcagatt tataaatcaa
14521  gaacaggata ctgcgatact gtcYtatttt ctgacaactt atattttgaa tagttgctga
14581  ctttatctcc taccccattc taaactcttc actatatcca ttgggatccc tagtaaacca
14641  ttagcagaag ccatatattc tcatttgttt ctctaacagt tcactttatt ttcttcattt
14701  gaactcgagg ttctggaact tttttgttca atggactact ttgccagtct ggtgcatgct
14761  ataatttcag aataatgttt ttaagtgcat gaaataaaaa tatttgaaat gtataaggaa
14821  tccatatata tatgcctttt tcttgcagta tgaaatatat tgttaatata atataggaat
14881  ttatgtgctt tttaattgac ataacaaaaa tatctggcag ataaagacta gtggtgaaag
14941  taaatgacaa taggaggtac ctgtaacgac aaaaattttt ggcttcctga agtgaaaaaa
15001  ttacagatac tgctggtatg gtatgtgctt gttacttaca ttcaagacag aagaaatact
15061  agacttaagc taaggattaa taaaaataag tatataattt ggtgcccctc ccagttcatt
15121  catcactcat gtccatgaat cttttcattt taatactcta caattaaatc ctgtggcagc
15181  tgttttctct ctcacaccct catagaagtg ggtctaaagc tgggatgggt gtttttcttt
15241  ccctctcgta actttcagat tactctactc tctttccttt ttcctttttt tttttttttt
15301  ttttgagaca aagtttcact gtgttgctta ggctgttctt gaactcctgg cctctagtga
15361  tcctcctgcc ttggcctccc aaaatgttaa gattacaagt gtgagtcact gtgcctggcc
15421  agattattct ccgtctaatt cctcacaaga catacagcag ttcattatct cctgtcatca
15481  ctctatacaa aatattacct ttccttgtca caatcataag ccagtcttcc ttccttgatt
15541  tttgctttct acctgaaatt aagtagttaa atatactaaa tttcaccacc attctttttg
15601  ctaaagttcc cctatctttt tgttggatga aataatcttc tattttttaa aaaaattcat
15661  tcagtaatct tgtttgttgt atttttcWtt ttttaaaatt taattatgga catataatag
15721  ttgtacagat gtatgggggt acatgtgaac ctcatgaaaa cagagcaata taaagacccc
15781  tgttgagaag ggataacatg aatgagaaat atgcatgtct ctttaagaag aggaacagtc
15841  ttaattaatg gataactata tagagtcaat aattcaagca taaaacactt tattataata
15901  gcatattggg aatagtcttg tctatttgta taaaaatggt gaagaaatag aatatttggg
15961  atccatggag acaaataatt tatttgctaa ataacaaaat ttggacaagg cttgaaatat
16021  atataacagt taagagcttc agctctggtg ccagacagct ggagttgaag cttattctca
16081  gttttaatga ttgtgtgatt taaattgtgc tgaatctttt ctttctccaa tgtgtaaaac
16141  attgatcata gtgctatcta tctcatagaa atattatact taaataaagt cttgcattta
16201  aaatacttag aatagttctt accatatata ctcaaaaaat tttaggtact attattattg
16261  aattttttgg cattccattc aataaatcac gattctctta tatagttgtt ttgctataga
16321  gcctttatta atgtgtctta attacaattt cctatccata atatttggcc aaatttcaca
16381  ttttatgatt gccatagttt gaatgtttgt attttcttca aaattcatgt ggaaatgtaa
16441  tccctaatgc aacaatatta agaggtgggg ccttttagga gatgattatg atgggtttgc
16501  cattatgatg ggattaaaag gcctgaaggg aactaaatag gtcttttaac ctttcccctc
16561  ttctgccatg aaaagataca gcattcaagg ttccatgttg gaagcagtga cgaagccctc
16621  accagacacc aaacctgcca ctgccttagt cttggacttt ctagccttca gcactatgaa
16681  aaaataaatt tctgttattt ataaactacc tcatctcaga tactttttat agcagcaaaa
16741  tggactaaga caatgccagt cacatgaaat cataaaatat ggcaacataa tagttatggt
16801  ttaacaattt gttttgtttt ccattactca ctgaaaatgt aaacaaactt aaatggactt
16861  agttgttttt ttccttcata tgtagttctt ttctttattc aatattaatg cccagtgcat
16921  ctcataatat taatggacag gtgactattt tgtttctctc tgcaatggca taaataaaat
16981  gattttaaca gcatttcaac ttaaacacaa taaattattt atttatattt gacaacacat
17041  ctaaatgttc aggttaagta aaaatattct cagatatata ggtttcagac gtttactaaa
17101  catagaactt ttttcaacat tttggaggct acattaacaa aaactataat ggggtgagga
17161  gatacctata tgaatatgtg gagtaaagtt tataagaaat tttgtaaggc ttaggatgtc
17221  aaaaaatttt ttaaaaataa gaaaagaaca tttgtatact ataaaaatct taaaactcca
17281  gttgtaggta gaagatcaac acataaaact Stgttgtgtt tctacacacc agcaatctga
17341  acagtgaata taaaaggtaa attaagaaaa caattccaaa ataatgaaat aactaggaat
17401  aaatttcacc aaagaagcaa aatacttata tacaaaaatt gatgaaacat tgctgaaaga
17461  cattaaggaa gacacaagta agtagaaaga catcccatgt tcatggataa gatgacttga
17521  tactgttaag atatcaatac tacccaaagc aatctaaaga ttcattaaaa ttcctatcaa
17581  aaatttctac agacttcgtt tcagaaagag gaaaactaat gctcaaattc aggtgaaatt
17641  acaagggaat ccgaataccc aaaacaatct ttaaaaagga gaacaaagtt ggaaaactca
17701  catttaatat ttttatttta tatatatata tatacatata tatatataca tatatatata
17761  tatacacata tatatatata tatatatata tatatatttt tttttttttt tccctgtatg
17821  cctcattaag Kcttccctga gccctgggct ggcttcttcc atggaagata gcagaagaag
17881  caaggcttgt ccctgttcac agaagatgct agccagatca ggagctgaag atagcaaaag
17941  agggagaagt ccaacccaca gtcccaggcc ctggtgagta aggtctggtg cgaggagaag
18001  gctgatgata tggaagggtg ctccagtcct gcttcttctg cttggaaatg gagaatccgg
18061  agaacctggg ggatgcaaga tggacaggct gctccttgag aacatccagt ctcctagaaa
18121  aaatgggtgc tctcaaggtg actgaatgaa ctcaactatg ccttgttatt ttaaaaccca
18181  ctgtctgctt tctgaattga caatcaagaa tccatgtgtg tcttaaacaa caattgttca
18241  ttgtagtaga acccactggg agaagccttt tggctcagga actccatcta gccacagctg
18301  attgaattag gggtggatac ctaacccaag tggagccaat cttattctgt gcccactgca
18361  atgtaagatg taaaataggt atttttgtct ccttggatca gtgctgtatc tttagtgtct
18421  agaattctgc ctaccacaca gtaggggcta catgaatatt taatgaacaa atgaatgaat
18481  aaataagtct ctgtgaggaa tttggaattt tgaccttgga aacacagaga atggcagttg
18541  tggaaactga gtcacattgg ctgcataagc tagaaagaac acccactcct tcccatgtct
18601  gaggtcttcg ggtctatctc agtcatgact gagtcctgac tctttctaaa gatgattgga
18661  ctcctcttaa ctttaggaca gatggctcaa catcctaact ataaaactcc ttcctccttt
18721  cctgcttaca ttcatttctg ttgcaaccaa aaacctttaa tgacatgctg agaaattttg
18781  caccttgaca tattctgttc cccaccttag gagcttctct aggagtcata atcactcctg
18841  gctgtacctt gggctgcatc cagatctgcc ccaatgccac cacccccagc tctgtgggct
18901  ctggctctaa gactacaggg acccagaagc aggaaagagt ggcatcatgg aattccatgt
18961  cctttagggc aggtgggagg tactatgcct cttctccatc caagaccgct cactacatcc
19021  tgtcctcaga tatggctctt gggaccctca agtcagatac tcctaatttt tcatctgctt
19081  ttgctctaca gacagaaaga ggaggtgcca aagaaatcta cagattcatt caaatcctta
19141  ttaaaaattc tcacagactt tttttttcag aaacagaaaa attgatcctc aaatccataa
19201  gaaattacaa gaaaccttga atttccaaaa caatcttaaa aatggagaac aaagttggaa
19261  ggctcacact taatatttta aaaattcact acattacttc tcagcctttt ggctaagatc
19321  aagtataaaa cttactacaa agctatggta atcaagactg tgttttattg gcataaggat
19381  agacatatag atcaatgtaa tggaattgag agtcttgaag taagcccata tatctatgac
19441  taattgattt tgacaagtgc gtaaaaattc attagggcat aaagagtctt tttgacaaat
19501  gatgctagga caattagata tccacatgca agagaatgaa gttggacccc taactcacgt
19561  catatacaaa acttagctca atatggatca atgacctaaa tataacaact aaaactaaat
19621  acacctagaa gaaaatttag tggtaatctt tatgaccttg gatttggcaa tggattctta
19681  catatgacat gaaaaaagta agccacaaaa gaaaaaatac ttaaattgga cttcacgcaa
19741  aataaaactt ttgtgtatca caagccactc caagaaagtg aaaagacgat gtatagactg
19801  ggagaaaata tttgcagatc atatatctgc gaagggctta aaattcagaa tatgtaaaga
19861  acctctacaa cccaacaaaa aatccaattt taaattgggc aaaagatctc aacggacatt
19921  tctccattga tttacaaatg tctagtaaac acataaaaag acgctaagca tcattagtca
19981  ttggggaaat gcaaaccaaa accacagtaa cRtattactt cacatccact agaatggcta
20041  tagtaaaaat cctggaaaat aacaagggtg ggcaaggatg tggaaaattt ggaactctcg
20101  tacattcttg gtgggaatgt acaacgataa atctactgtg gaaaacagtt tggtgactcc
20161  acaagaagct aagcatagga attgctgtat tatccagtaa tttcactcct agtatacatt
20221  gaaaacagga actcaaacag atacttatgt gccagtgtat attgcagcat aattcacaat
20281  agcccaaagg tagaaatcac ccaagtgttt ttcaactgat gaatatctaa acaaattgtg
20341  gtgtatacat acaataaaat attattcagt catacaaaag aatgatggtc tgatacatgt
20401  taaaatattg gtgagcctta aaaacatgct tagtaaacta aaccagacat aaaatgagaa
20461  aatctgtgtg attctactca tatgaattgc ctagactagg ccaattcata aagccaaaga
20521  ggagatcaga ggtcaccagg aactgtggga aaggaaaaat agggagttac tgcctaatga
20581  gtccagagtt tctatctgtg gtaatgtaaa agttttggac acagaggtga tgcttgcaca
20641  acattgtgaa tgtagttagt gccacagaat tgtacactta aaatggttaa aatgacaaat
20701  ttgttatata aatgttatca caataaaatt aaaacaaaat aaatgtgtaa attaaaaaat
20761  taatattcca gaccataaaa acatgcagca tggaaatgac tgtcattaga taagaacagg
20821  ttaaactttt aattttatat gaaatgaaca taaagatacc aattttaaac ccagcacatg
20881  aaaatttgat atcaatagaa acaggaaagg aaaaagatct gcaataaagc attaaaatag
20941  agtaaaaaga agaaatgaga agatagaaaa caatgcaatc acaattgtat tgagtgtaaa
21001  aatgtttctc aatagtaatt ttgagattac attattaaat aaagtgtcaa aaatgaaata
21061  tttgttttct acaagagaca tttaaaaata aaatgcagaa gtgttgaaaa tgcagaggtg
21121  gaaaacacac aatttagaaa tgataaatgc ttgaagtgat ggatcctaaa tatcttgact
21181  tttattacac attatataca tgaaacaaaa tatcacatat accttacaaa gttgaacaaa
21241  tattatgtat caaaataaaa taaaataaaa tgtaacaaaa ttagggaatt taccaagggt
21301  gaaataaaat ttaaagcaaa aattttcatg gcaaactttt ttaatatcag taaaaataac
21361  cataaaaaag taatgaccat gaataaaaat gcaattatca acattgcttc aaattaaatg
21421  caactacaaa taaaataatt atgagcataa agaaataaat cagtaattgt aagtgtaggt
21481  ttactaattt aactattgga aattgtcata tattaaagac gtaaacaagt aatatcatta
21541  atattgttca tctaatgcat atacatttaa ctttaacaca tcaaactcat ttaaagaaaa
21601  tcattcacaa aaatggatca tgtattgggc caaatgaatc ttatcctagg ccaaatatca
21661  tacacatatg ctgtctgact caaataaaat aagatatcaa tacaaaagtt acctttaaaa
21721  atctcattat tgttaaatat ctcttgcatt aaaaggaaag tgtaaagaat attttgaaat
21781  tatttgaatt gaatacaata taagaaccat gtgccaacat ttgtggaata catataagtt
21841  gatatttggg cagaaaacat acaattttga atacctttac taaaaaaaaa aagaaagatt
21901  ggacaaagct agtgaaacaa acaacttttg ccttcaaaag tatggcaaac cattatccta
21961  agcaaattaa cacagaaacc gaaaaccaac tatcacRtgc tcttgcttat aagcgggagc
22021  taaacattgg ttcacacaga cgtaaaaatg ggaacaatag acactggcga ctaagtggag
22081  agggagggat ggcaggggtc agggggcaag gactgtcaaa ctacctattg agtaccatgc
22141  tcactacctg ggtgatgggt tcaattatac cccaaacctc agcatcaagg aatataacct
22201  actaacaaac ctgcacctgt accccttaat ctaaaataga agttaaaatt atttttataa
22261  attaaaaaaa tctgtataaa aatatatgaa aaaccagatg tctggaaaaa tccttccagt
22321  caggaaaaca taaagatact gaaaaagaag ccttcagaaa tatttttaaa gtgtatggtc
22381  cagctgtcaa gagagttaaa aaagaatcct cagatgccaa aaatagtagg aaagtgcaaa
22441  tcatgagagg aaacagtgct gaagatagag ttagtcccta actaactatc ttggagttgg
22501  atagaggagt ttatcgagga gacggcattg tcactctcct ttaaaccagt gtgtaagtat
22561  tttttaattt tgttatgttt ttaaattgat gttccgcagt gaaaaggtat atgcaaaact
22621  atccctaaag gctgagggag ctgagaggcc aaagaaagag tctaacaatt ccagtttctc
22681  aaaaagaaat atttattgat ttatttataa tttttacttc aacaactttt tgggtacaag
22741  tgtttttttg tcacgtggat gagttatata gtggtgaatt ctgagatttt agtgcaccca
22801  tcacctgagt agtgtaaatt gtacctaata tgtagctttt ttatccctat cctcccttcc
22861  Rgcctccccc ttttgagtct ctaaagacca tgatatcact ctgtatgtct ttgtacactc
22921  atagcttagc tcccgcttat aactgaaaca tacagttttg gttttccagc catgtgtgtt
22981  acttcactta gaataatggc ctccaggtct atccaagttg ctgcaaaaga tatatatatc
23041  ttatacatat atatatctat cttatacata tatatatctt atacatatat atatcttata
23101  catatatatc ttatacatat atatacacac acaatctttt ttaaaagaaa gcaatattta
23161  ataggaactt ataaacagag gcaatgtctt gggtggccgc aagatattgg atccccacag
23221  ccgccctcca gaaaatatcc tttatatatt aagctttttg ggtaaaacat gtgcagctct
23281  tcatacctca ggctctcttg ctaaaactta tgaccattaa gaaggttaga taagcatctt
23341  tatgaggggc tctctatgtc acaggaatta tttaaagaac ttgctgcaga acacattggc
23401  atgcaggagt taaacatcag tcgttgtggt agtttcgctt caggatggca tcactctttc
23461  tacgcaataR gctattttcc tacgttatat aataattact gtacacattt atcaRgtaca
23521  tagtgatgtt ttgaaacata caatgtatag tgattcaata ggttaataag aagatctatc
23581  atcttaaacg tttatcattt ctttgttggg aatattaaaa tcctccttcc tggtatttaa
23641  aactatgtat tattgttagc tatagtcatc ctatagtcct ataaaacact agaacgtatt
23701  cctcctatct agctgtattt tgtatacttt tacaaacccc tatccttccc agcctctggt
23761  atactctgct cttcttttta cttctatgag atcttctttt tttagtttcc ttatatgagc
23821  gagaatatgt ggcgcttaac tttttgtttc tggcttattt caRgtaccat aatgtcttcc
23881  tttccatcca tgttgtagca aatgacattt cattttttaa aaaaaactgt tatggctaaa
23941  cagtattcca ttgtgtacat atacagcatt ttcttcatcc attcataaac cagtgcttat
24001  gttttaaaag accatatgca tgttggataa gatacctggt ttggacttct ccaaagttag
24061  aagtgagatt ggatactatc aacaagccaa gatccctgaa aggcaaaMcc ttaataggca
24121  aatgagaaaa taatataYgt ccaacaaaag gctaaagtag gaatacttaa cactcagcca
24181  gggcagtgaa ttgaaagggg aatgtaaagg ggaatgaaag aggaatgttg ctctaagaat
24241  ttgttataat agcctttcac tcatgtatat ttggggctag aagttgaatt ctacatagcc
24301  taaatcatta caaactgaaa agtttagctt aaagaagttg atactggaag tgatcccagt
24361  cagcctttac aaagtcaaac tttttctggc agacgtcact gtaaacctga tctcaaagaa
24421  ttcccacaga taatattcta ataaaactga gttcaaaata aaatcataaa acatgtgagg
24481  accaaaccaa tataattgaa agttagtaca aacaacaaag ttagtaatca gagctacaaa
24541  ggcagcagat atcagaatta gagcttctgc agtctgtgca aatgctaagg aaggttcttt
24601  ctctttccct tgtttctcaa agcaagtatg actaaacaga tattttttca caggcctcat
24661  cttttattta attatatttt tcatatgaca taaattattg catagaaaat tacataatac
24721  tacaagaaaa taaaaattat ctataatccc aatatctaca ggtaaagaac ctagacatcc
24781  agtttatgta taaatctaat caaaacagat tttttaagta acatgcttat tatctgtcat
24841  ctatctatgt tgagatctat tttacaacgt atgtagaaac atgttaacaa tggttagttg
24901  agtactgcaa atgtgcataa tcaatttctt ctttatatat ttttgtgttt gctaatttta
24961  gatgaaactg tattttatag tacaaaatgg agcttttgat gaaaggaaca ggtatcagta
25021  aaaataagct aatattggca aaaatagcag aagaaaacat aaatcaatat gagatcatgt
25081  aaattaccca cctattgccc caaacattac aaactagtta cttactaaac agcaaattaa
25141  gaaaaaatca taacacatat atggcatagt gttaatgtat aaactagtca caaaattggt
25201  aagaaaaact ataagacatg aaaaaataag gagaaaacaa atgaacaatt cccaaaaagt
25261  atatgctatt ggttaatccc gtagtaatgc ttaacttcac tagtaatcaa atatatgtga
25321  agtaaagata tttttgcaat caaagtagtg acattaaaaa taataacctg tttgattttg
25381  agactgcaac aggacggata ttttttcact attaacatat aggtttatta tatctacaga
25441  gcaatttatc aattctgcaa ggaatttatc aagtttctcc ccattggaat tttccttaaa
25501  aataatttac atactgctgt ctctatatat tagtcacact ggacctttag tttctggact
25561  accctctact ccttcttgct tcaaggcctt gacatgatgg tttcccaccc caaatttctc
25621  ctcttttgtc caccatactc ctacacatta ttaagatatc aaattaaacc atcttaacca
25681  cagtgacatc ttgtccattc tcatgaccag gtcaggtcag catcctatag tttttctctc
25741  ccaatatcat aaatatcatt gtttaagtta gctagctatt catttgtttt ctattagaat
25801  atattatcca ttatattggt atgttgttca ctactatatc ctcaatacct agccaagtta
25861  gcattcaaat agtattcata tgaatgaata gtattcactc tctataatac tcatattatg
25921  tttatttcta aaagtggaaa actgaagaca acttaaacgt aaacgatatg ataaacacta
25981  agtatattat tgtatatttg caaggggagt tattatgtac cccttaaaaa gattattaca
26041  aataattttt gaaaacatgg gaaaatgaat aaattctaca taatcaacat aatctataca
26101  gagaacatag ttctttatac aatataattt caaatgagcc aacctggtat atttggttga
26161  aataggcagc agcattaggt ttttttttct gttatctctt aactatctgt acattctaaa
26221  cacaatagtt taaaaatata aaaatattga tttggaaacc tgacatagaa aattaattaa
26281  gtccttatat ttggaaatat tccactcatt aggccataag tatgagatca aggcactttc
26341  ggaagttgcc aaaataagtc actgagttta taacctgggt tggacataaa agttaacttt
26401  aaaactcaaa atttgaaacg tttctgtgag tgcaagtttg tgtatatgaa tgtgtgtgtg
26461  tgcatgttYa tgtgtgtgtg tgtatgaagc ctgaagacag aacatttcat atttcaccca
26521  gattctatcc tgtgttcatc ttgtgtcctt tggtcaggga ctgtgttgaa tcttgcagcc
26581  tttggatcga tgcttctaga aaaatcttta ctcaacttta attggttcat aacctgcctt
26641  atctcaaatc actgattttg aagagcatat ttatttcatc agtgttttga tcccatatcc
26701  ctcaactaga cccttgtttc cctaactata tttcacagaa ctacatctac tcctctgact
26761  acttgctaca catcgtacac cagtaaaaaa ggatgttaga ttctaaaaaa tgaaaaaaaa
26821  acctttaaat aattaaaata gcattctgta aagaagtacc accatctatc ctcacttcca
26881  tgttctaaat gtcaagattg atctaatttt ctttctttct tttttttcct ttgggttttc
26941  tgggagaatg ggtgtccttg cttcagaaaa gtcaggtaaa gcacaacaag aagttttatt
27001  ttagagctta ttctcaccgt tataatcttt acagcgaata gtctccaggc tgatggtctt
27061  cctctaggaa gtttgtagga agaagttgca gggtggggaa gctgactaag ccactgaatt
27121  tgcatgtgag attaaaatct cctcaaagac tgagattccc tgaaacatag tcaaaacaat
27181  tatggaagat ttcagtgatc tttaatgaca cagttaaaaa gacaatgcaa gaatgcagtg
27241  caatgaaaca gatcttcaag gatttattca ctggattcct ttcgtggaac atccatctct
27301  tcccaaggtg tctatgtgac tctgagattt ttccttttct cacagcactt caattgctgc
27361  agtaccctca aagaggcttg ctttagcttg ctgtttccta gaattaagat aaatgagtga
27421  ccccagggat agatggctgt ggttgtcatt ccaaacataa gcagcagttt gttttctcgc
27481  acagtaaaac atgatatttc tatggccatg cctataaaat acaagataaa gaggatgatg
27541  aaagatatca aaactttcat tgccttcaca tgagcttctg tgttggagtc tctcaatcct
27601  gtcacattcg attgcatctg cctgttgtgt ctccaaaggg aaatgattaa aaaaatacat
27661  gtaattaggg atagtgtaaa gaagaaaatg actcccagat ttagcaaaat ctgtttaata
27721  aagtattcac ttttatacat gttgagatcc cagactgtgt cattcttcRt tttataatca
27781  ttaagaatct tcgcaatgta tgcaaaatta agtaacgatg aaataagtaa gaatactatc
27841  atgaagggaa gaaccatatt tgttctgctc ttcaaccaga gaaatatgta gttggaaaaa
27901  tttgctatct tcaggaaata gaagatgctg aggctggtgg caaaccacat acttgattga
27961  ttaccaatta cccaaaagta actaatatat tcaattaggt taccggaggc atatatattt
28021  ggagagaata tctgtataaa tccatctgta attattatcc atatcagaaa aattcttgaa
28081  atagctaagc cggtgagaat aaagccaatc gtagataact tattcttggc acagtcaatg
28141  cagtttacaa gtccaataaa tccattcccc aaaaccccaa acactgactc actaactaca
28201  acaaaaatga agatgccttc cactacacgt agcatatctg ctaattctta atattgcttc
28261  tgttacatct atcttagatt acctgctgca gaatgaggca tatattggct gctcgacgga
28321  agtgtgactt tcttctcttt cattatataa agacttgaaa ttgccccagt aatggcttga
28381  gatggaatca gaaatttctt aatgaagacc tttccaaata tgtctacaaa acatagctag
28441  tctggagaat gtatgttcag actgaatgcc tatctataat ttgttaaaat gcaaatgaaa
28501  gtttctgttt catggatttt acatgtcctc ctgagactat tttgcattta ataaattgaa
28561  ttgtggagga ggagtgccaa acatatacct atagaatgta atgcaaaaca gaaacaaaaa
28621  ttttgacttt atcttttaaa atatcaaatc attatttatg tcatttataa tcaatttata
28681  tatgtacaga aaatactata tagaaatgaa aaacaaattt agccactgta gatacttact
28741  ctaaatgatc gatttaattt ttaattaaaa tttaagctca actctaggta tgacaagttt
28801  cacaaatatg tcacaaatct gttgctgttg ttttatactt atggttatta cacccttgaa
28861  tgcagaagat tatgttgact acccttgaca tgaaaggctc ttaggaataa tctcttcatt
28921  taacacatta ctgtttgctg gcacccattc atttatccaa caagtattta ctgaatgtct
28981  agatacaaca agctctcttt tgatctttga gggaatatag tggtagacag aatttcaaaa
29041  cttgtgtgat ttatggagtg tatattctaa tgagggaggt gggaaacaat taagtaaatc
29101  aacatactga tctaagaatt aaaagtgaaa ttaaaagtga aatctaacat ttattgagct
29161  tttattatat tcctagcgct atgttaagta tctttttctc atcttaattt cacaagcctt
29221  caatccttta aaagaggggc tatttttgta tttacattaa atatgaataa actaagcttt
29281  ggagaaaaga agaccttctc tcacttatcc tggcatgact accataaata aaattgcaca
29341  atgatttcaa aatgaagtta aattaagctt aggaatagac tacaaatgat agcaaaacaa
29401  aacaaaccaa ccaaaagcaa gtagaaaact taaattctca tgggctgcac atactgaaat
29461  tgacagataa gaatatgtat agataaatta gctaatgtat acactgttta caaaaaacaa
29521  gatgcaattt cagagaagag cagcaagaga ctgttagaaa gaaattggaa catttgggga
29581  aaaaacttct agaaatggag aatataatta tataaaatgt aaatgaacta aatactccaa
29641  gtaaaaaatt aatagtaaca aagaagaatt taaaactctg acYgtatact gcttacaaga
29701  aacacaattt aaatatagaa aaggaaaact ttaaagtaaa attatggaaa aataagccat
29761  gcaaacatca tccaaaagaa atctggcata attataacaa tattctataa agcagacttt
29821  aaggtaagaa gcagcattag agaagaaatg gggagtgtac tttatagtga taaaaatgct
29881  caaatctaca gaaaggtagg gtaatcccaa aaatttatga accaaataat ctaccaaaca
29941  tttacagaaa aggaaaaata agacaaatct acaatcacaa tgaaagactg taaaatacat
30001  aactcaataa ctcatcttag aaacaccaaa aatcaataga tggaaaatac atgaaaaaac
30061  aattgacaaa cttgtgttca acggtaaaat aattcatatt cttttcaagt gcacatagac
30121  tatttttacc aagactgatc atgaatccaa aagaatatct cacaagtgtt gaatgttctc
30181  tgaattcaaa ggaataaaat gaagttcaat aataaaaaga taactagaga aagaaacaat
30241  ttactaagac aaaaaacctg taaatactct tgagtcaaag aagaaataac aaaaattaca
30301  aaaatatttt taagtaaata atacaggttg tgtatccctt atctgaaatg tgtgagacca
30361  gagagctttg gatttcggat ttttttttct ttttttttaa gttttggaac atttgcatca
30421  tactgattaa gcatctcgaa ttcaaaaatt tgaaatccaa aatgttccaa tgagcatatc
30481  ctttgagcgt Matgtcagcg ctcaaagagt ttcagatttt ggagcatttc aaatttcaga
30541  tttttggatt agggatactc aacgtgtaat aaagaaatga cctattaaaa tttacataat
30601  ccaattaaag ccattctttg aaggaaattt gtagccttga atgcatcttt tagaaaggca
30661  gggtaaaaac agatgaattt tgttttcatc tcaagaagct agaaagctaa gagtgaaaga
30721  aaatagaagt caagaaatta tagtWacagc agaaatcacc gatagaaaag aattaggtac
30781  agtaatatac taatgttggt caaaagaaaa tacaaattac tacagtcctt agagatatca
30841  gaaatatggc ctgggtgtgg tagctaatgc ctgtcatttc agcactttgg gaggcccagg
30901  tgtgagaatc acttgagtcc aggagtttga gaccagcctg ggaaacatag caagacctta
30961  tctctactaa aaaaaataaa taaataaaaa taatttagca gggcatggtg gtgtgcacct
31021  gtagtcccag ctacttggga ggccgagcag gaggattgcc tgagcccagg agtttgaact
31081  tgcagtgagc tataattggg ccactgcact ccagcctggg tgacagagtg agacctcatc
31141  tctctctctc tctttctctg tctctctctc tctctctcta tacatacata tatatacata
31201  tatatatata catatacata tatatatata tataatacta tataatatag ttacatatcc
31261  ttgtatataa tcttttatta taagacaata ctgtgaacaa ctttatacaa aatttcatca
31321  agaatactta taagactatt acaggctaat atagcctgaa catagatgca aaaattccaa
31381  acaaaataga gtccagcgat atataaaaga ataattcatt ataacaaaYg gagttttaaa
31441  agccaattgt gagattaaaa tgaaataatt gaagatacag tcaattaaca caaaagaaag
31501  ccagaaaaag gacagaggga caaaaataag tgagataaat gaaaaacatt gagcaaaatg
31561  caagacttac agccaaccat actgataatt acattaaaca tgatttgact aaacactctg
31621  atgaaaagat agagatcatc agaatgaata aatgaaagca caattaaatg ttatgcctag
31681  aagataaaac attaaacata aaggcacaga tagattgaat gtaaaggaaa gaaaaagcta
31741  tactatggaa tttgtaacca tagaaagcag gactggttSt attctcaaag tggctatatt
31801  atcaagacaa gtagtagaaa aatagaggga agttgtataa tgataaaata ttcaatttat
31861  caaaaatact taacaatcct gaaggtgtat cagactaata attgtgactc aaaataaata
31921  aagcaaaatt aacagaacta aagcaagtga taggcatagc aaagatttaa aaaccccttt
31981  gctgtgatct gcatgtgtct cctccaaagt ctatatgttg aaacttaatt gccagtgtgc
32041  cagtattaag agatggttcc tttaggaggt aatgagggca gacctctcat gaatgagatt
32101  aatgaccatt ttgatgtgca ttctaggtat gttaactcta catgccttag attgtgcaat
32161  agtaactggg ctcttatgaa agggttttaa caaMttccac aagttctaga ctaatttctg
32221  cagttacatc agcagcaaaa atattcacaa aaacaatggt gccactgcat ttcagcctgg
32281  gcgacagagt gagactccat ctcaaaacaa acaaacaaca aacaaaaaac ataaagtaac
32341  tggctaaatc catattatta cagagacaag attgttacct agaggaaata agtggttttc
32401  attctatttt gcttacttct gttaaatttc taacatccac Yttctgtaga tctRaatgRg
32461  tggattacaa cttattcaca cctctttttt atatataggt gattattttc tctttaaaat
32521  attttaaaaa ctattttaga ggtcagtcct ggcccaggaa gaatttattt attttatttt
32581  atttatttat ttatttattt atttatttat ttatttattt atttatttat ttttgagaca
32641  gagtctctct ctgtcgccca ggctggaatg cagtggcgcg atctcggctc actgcaagct
32701  acacctcctg agttcacgcc attctcctgc ctcagcctcc ccagtagctg gaactatggg
32761  cacccgccac cacgcctggc taattttttg tatttttagt agagacgggg tttcaccgtg
32821  ctagccagga tggtctcgat cttctgacct cgttatccgc ccacctcggc ctcccaaagt
32881  gctgggatta caggcctgag cccgcacccg gccaggccca ggaagaattt aaatggtact
32941  tactcagtga cacaggacat atacaaaaaa agagtacgat gaagcaagaa agattacacc
33001  aagacttctt gttgacttct ctctgaggcc tacctgtgta gctaagagtg cccgtccttt
33061  caggccacat ttcaggtgcc agagaatccc caagaaagtt ttcttcattt ggccgtatcc
33121  aaaaatcaca acatatgagt gacccaaaga atactgactt catatcataa accagatatc
33181  acaaccagtt tgttctgtag caaaaaatac ccctgtaaat gtcaagaaaa gggcaaattt
33241  ttatagagca acaagacaaa aaaggaaaac cgttttcttg gctctgaaat gggcccgtat
33301  actggggttc tgcaatccct gaaattgaac ctaatgtgtt tcatgtgtct tcacgaagaa
33361  aggagtaaca acaaaaagaa aatcgaagaa atagagaaag aagagagaga tccaataaag
33421  aagattatta tattattaac atttttatta ttacttacat tgagtaatcc agtcatatgt
33481  ctttcatatt tttttggaca cgataccaga agacatcaaa agtgtacgtc aaaggaaagt
33541  tggtaaacag aaaaggcaga taccagtgga agtatgaaag ctaccttgtt aattctccat
33601  tttagccaaa agtaaaagag ataggagaaa ttgacgatct ttaggaaata aaagacagca
33661  agacaagtag caaatcaagt gcacaagtgg ttggccagtc tccggacaat ggtaaggata
33721  tggtttcttt ccctagcatc agggattttc taatagaaga atattgattc tggctaaggc
33781  caaactagta agaatgaagc caaccatgca taacttctga tttctcatcc aggcaatgca
33841  atctgtgagt ccaatgaatt aatttcctac attcctttta taaatcccct acctattaca
33901  gtcatgaaaa cctccaatat attcaacatg gctacaaagc gaaaattctg atctgcagtt
33961  tgttgtgtga ctaattttca gatgacttac tggataataa catagatctt atgtttgatt
34021  gtgcagtgtt tttattttat catggctttt ctttttagct ataaagttta gcaaactgca
34081  gtaagacagt gaaatcactc tctgtaacat tcattgaaaa tttctaacta cctctgctaa
34141  gaatttccag tcttggtcca gctgtcagct acaaactgta aatttcttta tagaaagatg
34201  atcacactct ggggactgtt gtggggtggg gggaggggga gggatagcat cgggagatat
34261  acctaatgct agatgacgag ttagtgggtg cagtgcacca gcatggcaca tgtatacata
34321  tgtaactaac ctgcacaatg tgcacatgta ccctaaaact taaagtataa tttaaaaaat
34381  taaataaaaa taaaaaaata aaaacaaaat taaaaaaaaa gaaagatgat tgacaaacca
34441  aattatcatt tgctaacatg taaataaaga cacagactaa ttttcattgt tttgaataaa
34501  attagttctc ctcttccaat gaagtgtttc atttgattta caaatctgaa gtttgaaaca
34561  gaaacctttc accacattaa aattattcaa catgtaggga aaaccagcaa tccttatgct
34621  aattaattat aatgcagtca cagtgtagat tgctacatta caaagtaaaa tacagagctt
34681  gaaattttca agaaacttgg tgggcaatat atgaatctaa tatgagtatc ataattatca
34741  ggaaaacttc ccccaccgct ccccaccccc cactataaag atgcaggcct ttcaaataat
34801  ctgtcagtag cattttaaac acatttttaa aaatgatgtc tttcagtgaa acataagcac
34861  cccaaactaa acatggaaaa agtgaacttc ttttgtttcc tcaaaaacaa gattctagca
34921  ctttgggagg ctgaggcagg cggattgctt gggcccagga gtttgagacc agcttgggca
34981  acgtagcaaa accccgactc taccaaaaat acaaaaatta gctaggttag gtgtgttggc
35041  acacgcctct gatcccagat acacaggagg tggaggtggg aggatcacct gagcccaggg
35101  aggttgaggc tgtagtgagc cacgaaaaaa aaaatgtttt ttttgagata Waagagtctt
35161  caaatatttc cacagaactt tgttaaggtt acattgtctt gaggggaaaa tgcttaaaaa
35221  gtcatgaata tacttaggat gatctgttat gtgtatatcc atatacacag atataggtac
35281  atatatggct acatatacat aaacatatac atgtatacaa atacacacag acacatctat
35341  ttatacacac attgtactgt gWtttgtcct ctacttctct gtattttagc ttgtctggtt
35401  tttgttactt ggattgcttc tttttttgca ttattttatt atttttaata aacgcaataa
35461  ttgtacatat ttatgtgata cagtgtgata tgtgaataca tttatacaat gtgtaatgat
35521  caaatcaagg caattagcat atccgtttct caaatattta acatttcttc gtgttgggca
35581  cattcaaaat ttgtttttct agctcttgta aaatatacaa taaattgttS taattgtagt
35641  caccctactg tgctatagaa cactagaact tgttccttct acctggctat acttttgtat
35701  tcattaacca acctttggct atctccctct cccttctccc ctatcacatc tctaggaacc
35761  attattctgc ttcctacttc tacggaatca actttattag cttccacata tgtgtgaaca
35821  tgtcatgttt gtctttctgt atctggctta tttcacttaa cagatgttct ccagtctcat
35881  ggatgcttcc ataaatgacc aaatttcctt cttttaatgg ctaaatagta ttccattcat
35941  tatgtgtata tatcatgttt tctaatccat tcaaatgtta atggacactt aggttgattt
36001  catgtctcgg ctattatgaa tggtgttgca aaacacatgg gagtgcagat atcacttcga
36061  catattgatt tcctttcctt tggatacata cccagtggtg ggattgctga atcatatggt
36121  agttatattt ttagttttta agaacttcca ttctgttctc cataatgact aaactaattt
36181  atatttgtac caacagtgtg taaaacttcc tcctccacat cctcacaagc atttgtgttt
36241  ttttgtcttt ttgaaaatag ccattataac tagaataaaa taatatctca ttgaggtttt
36301  tatttgcatt tctctgatga ttagttatat tgagcacctt tttatatacc tattggccat
36361  ttgtatgtct tcttttgaga gatatatata cagttaattt gcccatgttt taatctaatt
36421  atttgtgttt ttgactgagt tccttctata gtctggatat gaattccttg ataaatgaat
36481  agttttcaaa tattttctct tattctagag gttgtatcgt cagtctgtcg attgtatatt
36541  tgtgtatgct tttatcaaat caacataatg taaactaaat gagataaagc taaaatggtc
36601  atcgcttgct taacataaaa tgcattattc atctaatttt attgttacta ttatataata
36661  ctataatttt aattttgcca ttgattatat agttgcaatt ctgatggaaa cagtgaaatc
36721  actttctttt aacttatttc aggcagataa tttcatcaat gaggctcaat gatctatcac
36781  caaacataat aaaagaagtt ggattaaatg aaagggatgt gtgctgagtt atttgttgtc
36841  ttcatttttg agagaattac caatcaattc aggtgtgttt atgaggattt agatttcctt
36901  atattcatgt aacaccgaag gttcctcagS acacagagag aagcctgttt cagtttgctg
36961  ttccataaaa tcagaaaaaa atgaatgact gaatagatag aaaaatgcta actcaagagc
37021  aaaaatctgt gccacaatgc tatctagatt aaaataattt cgctttataa tcgtattgct
37081  caaatagtat ataataaaga ggagcaggaa tgaaattata gatattaggg gatgtcaaac
37141  ttatccttgc attcttattg tacaaaattt cctcagttta catttaagcc cacatgtaaa
37201  attacagcag aatattctgg aaattgccca acaggtgaga ataaaatcta tcagggagag
37261  tttccagcgc ctgatccaat caatgcagtg aaccagcacc ataactctac ttctctgaat
37321  ccccattatg aaatctatag cagaaatcat tgtaagcata ttcttcaaca tgtttgccat
37381  gcttgattat gggatctctg ctttctgtac accactgatt tataaataag atgttataaa
37441  tttgtgtatc caaagcttta gtgttcaata tgtttcttta tcctgtgctR ttgattgaca
37501  ctggaaaaga atggaatgcc tccagtgtgc tcccagacaa agtcctagtt gtctttgaaa
37561  aacgaaaact acctttattt ttctcagaat ttcagctagt gattttcaag gcagatatta
37621  tattacctgc tgttgtgaca ctgtttataa tttccttgta aagctatcaa ctaggatttg
37681  aattctgatt tgttaaaata caaaatagag attttaaaat attttttgca tctttttttc
37741  cttaagattt aaatgttttt tattcattca ccttagttat aagctagaaa cattaaaata
37801  tggtgtcttc atggatctcc tgagaagcaa ggcagtcatt gggataaatg cctatatgag
37861  aaagcaagga gagagcgaaa ggtgaacaga gctattgtac taggatataa ctttgacccc
37921  tgtgaaggag agagagaggg aaagaaggaa ggtcttaggc agcagtgcag tcctgagaac
37981  atttcaagat gtccagtagg gagttatcaa gcccaaggtg caaattagag gagtcctgag
38041  tctcccagga gcaggcctga ccattgaatc tgcccccttc tctgtctttg gctgaaagcg
38101  gctcatggaa gtgaatgggg tgatagattt catagcatag gtgccatgct tgttagctgt
38161  tggggaaata cgattaaaaa caaagtctcc tcccaaccca gaaagtctct ccacaaatgt
38221  aatagagaaa gaaaatagtt ttcttattaa ataaatatta aaccagaatc tgatatacat
38281  tacaggcaac ctactaagag actgcaaaga cagaaagaga tctcacccgt ttttatatag
38341  ccaatatatg catatgaccc aatatataca ttttctcaag ttaagtgata actaatcccc
38401  aagagagagg acttggcagc attatttatt aYgcatagtt tatcttaaat ttacctggaa
38461  attggggtgg ccatctgtgt tagttaacag actttaaaca aaggacaaat aaacacagat
38521  ctttatgtag gagatagtct tgccatttgg agcctggtgg ccattgaagt taggctcctg
38581  ctcccagcag aaactgagag ataagggtgc ttatttgtaa aagagatgat taagcttctt
38641  tatttttagt gtgtatttgc ccttctgcag ctaattgtgc tccctcaRga cagagatttc
38701  agaggcacat tcctatcaca tgccacaaat gtctaaaatg acagtttatc ttcttactca
38761  cctcgccaat acatattatg tataaagtac ccaaggttta agtaaataca caaaatctgc
38821  tttctgatac tatatcagga tcgcttagag tctgtggcag ttttgaaaca tggctgcaaa
38881  ctctttgatc ctcttccttt tgaggcctag ggtctgcaga ccttcctctt aaaactgagt
38941  gggcttgtaa ctgctttaaa caatgacgtg tggtggaaac gacatcatat actttctaag
39001  gctatcaaga aaaactgtat ggcttttccg ttactcactg gaaagtttcc tcttacaatc
39061  ctgaaccact tcataagaag tctgacaaag ttgagaccag catgctgtga ggaagctcaa
39121  gccacacgag gaggccacat gtaggcaata tagtcaacca tttcaaatga gcccgtactt
39181  ctgatcatcc aagtccagtg atagacatga gagtaaaggt ctgcttggtt attgcagccc
39241  ctgccacttg agtcatagtc cgctgtctac atattctaag gtgaaggtct agacattttg
39301  aagaagagac aagccttctc atagtgccct gtctgaatta ctgacccaca gaatctggaa
39361  gcataataaa gtactgttgt tttacaccca ctagggccta ttggggtggg ggttggggga
39421  ggaggaacat caggaagaat agctagtaga tgttgggctt aatacctagg tgatgggctg
39481  atctgtgcat cagaccacca tggcacatgt ttaccaatgt aacYgcacat tctgcacatg
39541  tacttcagaa cttaaaagtt gaagaaaaaa tacacaaaat aaaataaaat aaaatattgt
39601  tgttttgtat ctctgagttt tggactcagg gtgttttaaa gatcaatgca taactaaaat
39661  agaattattt agcacataca taaggaaagt cttttactag cttagttttt attttcttag
39721  catcattatt tatagtagcc aaaagataga aaaaaaccca aatgttcatc aatgaataaa
39781  taaatagaca aaaggtggtt tagtcatgta atcaaatatt atttaactat taaagggaat
39841  aaagtactga tacgtacaac atgtatgagt cttgaaatta tgctaagtaa aagaaaccaa
39901  tcacaaaaga tcccatatta tataatgcca ttcagtcgaa agtccagaat aggaaaatat
39961  acagatgcag gaagaagatt aatagatgct taggctgggg tggggagaaa ggtgggatga
40021  tgggggaata gggaggtgat atctaatgga tatggggttt ctttttgaag tgataaaatg
40081  ttctacaatt gacagtggtg atggttgcac atgtctgtgg aatatattaa aaatcattta
40141  attgaacact aaatgagtga attgtatatt atgtgaacta tatctcaatt aaagcattta
40201  aggcaaaaac aagttacata tgacattttt ccttatagaa tatgtaacat ctgtgtatat
40261  ttttcataat tttattatac cttgtagtaa cactattagc tcagtcatct ttaattctgg
40321  tgaggtataa ataaaatacc aaaagcttta catcatttca cttttgttga ctattactta
40381  ttgagaaact ttttactctt tggatgagac atgataggat aggctatgct gtagcatcta
40441  caatgtaagt ttcatgaaag gttcatttct cggttcacat tacatgacta attgaattta
40501  aaaatgcaga gtgaggtgag gatagttttc atgtagtctc tcaggaatac aagctagtgg
40561  agtatctaac actaaacatg tgtctttcaa gtccctcact taggaagaaa agagacacga
40621  agagccttct tttacatatg aatattcaac tgttccaaaa tgattagttg aaaagataat
40681  cctaatcttt ttttttcagt ctttaagtgc tcttttcttt tattctcagc cccctttatt
40741  gttattatta ttttcatttc attattatta tactttaagt tctagggtac atgtgcacaa
40801  cgtgcaggtt tgttacatat gtatacatgt gccatgttgg tgtgctgcac ccattaactc
40861  gtcagatttt atgtttcttt atcctggctt gataaaaccc attgtctcag gacttttctt
40921  ctaaatagaa aagtagtctg aaggctcgtc ctgattcatt ttaagactgt agtatttctc
40981  ttctgccagt taatatgttt tcactttttc tattttcttt ttatatccaa tacatttcag
41041  tatgaaattt tgtgatggtc cactagtctg atgtgcacaa tttagccagg aataaatatt
41101  agtggattca aaataggcac tcaaactatc tgtccagatt ttttaagtgt tgttggtttt
41161  tttctttctt tttttttttt tttcaaattt caactgatga atgaaagtag ctgcctagag
41221  aataatgagg tgggtgccaa acctgggttc attgaaggcg ttatagcacc tgtgccatgc
41281  tataacttag ataagtgacc ttatctaagc ccatggcttc aaatgccact gaaactggtg
41341  aatttatctt ttaggaatgg aagggctata gagatatttg atgaaagaaa actaagagga
41401  tttgtcacca gaacatctac cttttaaaag ggggctaaag aaaattctct agccaagaaa
41461  aataaaaaag aaagtttcaa acaaaaagat taaagaaaac acaatggaaa ggaagaatga
41521  ataaattcat cttccttttc ttcttcagtt tggtaaatgt atttgacagt tgaagttacM
41581  attatgacat tatcagctgt gattgtaaac ttgatttcag taacatttga agccatgggc
41641  ttagacaagg tcacttagga atagtgtaga tatcaggaca agtaagacct ctgagacagc
41701  catgtgggaa ctcgacatag caagcaagcg atggggagcc ccacccagtg actgtcttca
41761  ctctagcagc tctcagtgaa ggcttctgta ttaggccatt cttgtgttgc cataaagaaa
41821  tagaggggcc ctgcatggtg gctcacacct gtaatcccag cactttgaga ggcagaggcg
41881  ggcggatcac gaagtcagga gattgagacc atcctggcca acacggtgaa accctgtctc
41941  tactaaaaat acaaaaaatt agccgggcgt ggtggcgggt gcctgtagtc ccacctactc
42001  agcaggcaga ggcaggagaa tcacttgaag ccgggaggtg gaggttgcag tgagccaaga
42061  tcgcgccact gcactccagc ctgatgacag agcgagactc catctcaaaa aaaataaaaa
42121  aataaaaaat aaaggtaatt aataaaagag atttaattag ctcatagttc tgcagggtgt
42181  acaggcatgg cactagtatc tgattagctt ctgatgaggc ctctggaagc ttacaatcat
42241  ggcaagaggc aaagggggag gaggcgtgtc acatggtgag agtgggagca agtagaggag
42301  gcgccacaca cattcaaaca accagatctt gcatgaactc agagcaagaa ctcactaatc
42361  accgagagaa tggtgttaaa ccaggagtgt ccaatctttt ggcttcccta ggccacgccg
42421  gaagaaaaag aattgtcttg ggccatacaa aaaaatatac taacgatggg tgatgagcta
42481  aaacaaattg ccaaaaaact caatgtttta agaaagttta tgaatttgta Ytgggtcata
42541  ttcaaagcca ttctgggctg catgcagaat gctggccatg tgttggacaa gcttctgcta
42601  caccattcat gaggaatctg ccccatgatc caatcacctc ctaccaggtg ctatcacaac
42661  atcgRgaatc acatttcaac atgaggtttt caggggacaa acatccaaac catatcagct
42721  tccctgtgcc aagcatggat ggagctgaga gctttatatg ggttacttac aaaatataca
42781  tgttttatac aataatatta atagtacaaa ataattactt tgtaccaaaa attaattgta
42841  caaaatatta aaatatttaa cataacattt gtacaatgta tataaaaata taaatatccc
42901  ataaaatttg ataatagcat atgctatttt atttctataa tatagacact cttcaatact
42961  cagcaacttt gttcacatta gaccactttt atttgtaatt attgatatgt tgggtcacaa
43021  acctgccatt ttactttRtg atttctgctt atccgttttt ttttcttttt ttgtaattgt
43081  tgttttgtac gccttgcttt catgtgtgtt actttaattt tcttttttta gaattccatt
43141  gaggtttatc tgtggtattt ttgagtatat ctctgtgtgc agacatctta gtggatgctg
43201  taggttttac agcacatgta ataacttatc atggtttaat agggtcaaaa ttttagtact
43261  ttgaaagatg tacggaaacc ttattccttg attccctata tgtctcttta ccctcctctg
43321  ttccttatat agttgtctta aatatttacc tatatacact taaaatcgca tctgataatg
43381  tcataatttt aacttcagct gtcaaataaa tttaccaaac tgaagaggaa aaggaagatg
43441  aagttattca ttttttccct ttccattgtg ttttctttaa ttttggtgtt ttaaagtttc
43501  ttttttaatc ttttttcttt ttttttttcc tggatagaga attttcttga gccccttttt
43561  aaaaggtaga ccttctggtg acaaatcctc tcagctttct ttcatcaaat gtctttatag
43621  cccttccatt cctaaaagac aaatatacca gatataaaaa ccagaattga aagttatttt
43681  atttcatcac atagacagtg ttgtgccact tttctatggc tttcatctat ttctgatgtt
43741  tcctgcttcc atttgtattg ttttctctcc tatagatgag ctatttcctc tagctgcttt
43801  caaaatttgt tctttctttt aattttcaga tattttacca tggtatacct tagtttggat
43861  tctttgtgtt tgtcttggtt agttttatgc actgaaagtt tgtgtcccct caaaattcat
43921  atgtagacat cctaaattca atgtgatgtt atttggaggt taggcctttg ggaggtaatt
43981  agggttagat taagtcatga gggtggggcc ctcatgttga gatcagaggc cttataaaaa
44041  aaaaaaagag agagagagag ggagaaggag acaagagata tctttttctt ttctccaaca
44101  tgtgaggata caatgagatg atgtccatct atagccaaaa aatgtcttca gcagaactca
44161  tctgtgctgg cattctgatc acRgtcttcc agcctccagt attgtgagca acacattttt
44221  gtttttataa accacccatt ctatggtatt ctgatacagt agcctaaaat ggctaagaga
44281  ttttgggttt gcttgaattt aggaatctgt gggtttttgc ctattgccaa attaaggaag
44341  ttttcagtct ttgagacctt tctcaaagtt gcccatttct tctctccttc tgggactctg
44401  ataacacaaa aattaggcct tcagttataa tactacaggt ccctgaggct gtgtttatag
44461  ttttttcctg cttaccttca tgaatttttt atgtggttat tgtaagtttc agttctaaaa
44521  tgtccattca gttctttcat atattttaat tttttggctg agataatttt taaatttgtt
44581  tcatatatgt ttgtaagcaa tatactggaa gcatttttat gatgactgct ttaaaatatt
44641  ttctgaataa ttccaacatc tttgtcattt ttgttctgga atcttctaat taccttttac
44701  attcaagttg agatttttcc tggttctttg tataccaaat gatttctaaa tgaaccagga
44761  cattttaggc attatgagac tctagaactt atttaccttt tctgtcttag ctagcttcct
44821  cagacactac tccagcaagg caagggagtg agggagggac actgcattac tgccaggtga
44881  tgatagaaat aaaggtttcc tctcaatctc tgttgacacc taggcaggcg gaggttcctc
44941  attactgctg gctggagggt ggattctggg tcaccactgg gcctccactg atagcctgtc
45001  tcggaggaag aagtgacttg ttactgtttc ccacatggcc tccacaagca ccacctggta
45061  tggaaaggag ggtgcttctt cattgctgct tttagagtag atccagctcc ccatttgctc
45121  tccagtcata tcaaggtgtg gaaggagagt gaggggctta ttaccacaag ttggggatga
45181  aagttccaac ttctgctatt ctgagatact attctggagg agggttggga caccttgtca
45241  cagcctggct ggtatgaaga tctaaagatc taagctcttc acttgaggta atagtgtttt
45301  ctgtggtact tgactacagt agagtggtta ttgtcgaaat gttttctgcc ttggtagact
45361  atttccttga cctttgccta aaataatagg cttttgttgg gaaattctca tatgtgcctg
45421  ttagtatttc tatgttgatg gcattttcag ctaaaagtct gcaaattata aagcagaaaa
45481  gaaaacttag aggattcatc actatacaat ttattaagtc cctaccacat ctgccttggt
45541  ctctccaggt ctgagttttc atatatttta tatataatgt tcagggttta tagtttttct
45601  tagtagaagg aatagggcaa aatacattta ctccattttt ctggaagcag aaattggttt
45661  actttctgta agaaagcagt tggtgaattt ctgtctaaat tctctgcctt ttcaagagga
45721  actgggcttt acttctcaga taacaactga gcaacgatag acctacaggt ggcattactc
45781  tctgtatagg tagagaatag gctgaatatt aaatgttaag gagcagaatc attggaattt
45841  aaatgaggaa ggagacttag tttttattca cgttaaccag ccacctgatg acatgcaaca
45901  gtgcgtttgt tcatcaacat accttttaga tggcctttct gcttcttttt tgaatggctt
45961  taatgctaaa gaaagcaagt gtttatcaaa taatctattt tttagataat tcattattag
46021  aagttaattc attattagaa agataattta ttattagaag gtaattcatt attagaaaga
46081  acaaaacgag tcttttttaa taaaagaata aataaattta aatgtattga acttttattg
46141  ttgtatgtca tgtagggtaa ttttgtatac atgttgcatt ttacaagact taaatcgtgt
46201  aattttatat acatcttgta gggtaatttt atatacattt tataaggtaa ttttatatat
46261  attatgcatt Waattcttgt aagtagccct ctgccttagc cagttcgagc tgctattaac
46321  aaaatatctt atcttgggta atttataaat aatataaatg gatttgtcat agctctggag
46381  gctggaaagt tgaagattga gatggtacca gatttggggt tgagtgagag cccattccaa
46441  acagacaatt tctcctggct tctctctcat gaggcagaag tatgaaagaa acatgaacat
46501  tgagtcccta catgtcaaaa gagatggaac agctcagcag cctttgggag cttctttttg
46561  tagggaaatt gatcccattc atgaaagcag agtccacacg acttagtcac ttcccccaaa
46621  gactccatct cttaatacta ttgcataggg aattaagttt caacataaat ttattttgta
46681  gagacataaa cattcaaact atagcattct gtccctggtc atcccccgaa atatatggtc
46741  ttctcacata ctaagtacat ttatttcatc tcccaaatcc caaagtctta actcattcca
46801  gcatccactt taaaatctaa atccaaagtc ttatctaaat atcatcaaaa tcagatatgg
46861  tgtggccaat tttctaatat tgaaccatct ttaacatcct catcctcttt caacgtggtt
46921  tattattctt ttagcatact gttcgtttgt ttatgtacta ttgaatagtt tccctaacag
46981  agtatccttg ctgtcctata tattttgttt aatgaaactc agaatgaatt cagaaacttg
47041  ttgtttcctg tacttaaaaa atctaaaaaa gaattatgtg ctttttaatg ataaagatta
47101  gatattaaaa ttttctgtat tcagcgacat tggaggtgat aatgagaggt agctggagac
47161  agttacatat caaattcttt tgcttcttag ggttattttc tattcaactt tgcctcagca
47221  acttgagtta aatttggtag tctatattat ctgacaataa tactgatatt gctggaattt
47281  ttacaatcag aacagagttt tactcaaatt tcttgccctt taaagtacat tatgctgcac
47341  cctttatgaa atccactcaa gtttttaaaa agaatggtga acgacttagg gaggaagaga
47401  gctgagcaat gaaaaaagag gagaaaagag catagtgagt tttatagaga agagatctag
47461  aactacattt atgcccctag aaaccaggat taatagtttt ggaaatttta agaattcccc
47521  agcacttgca actccctctc atttgttata tattccttgt tttcctagag ttacaagaat
47581  atcaatgaaa gatgtaaaaa taattttaag aatttgcatt tgtgctatgc aatctaggac
47641  agaaggggaa caatggaact ctttctcctt ggcagctggc tttattaaga taaaggagga
47701  gggaagcctg ctggaagcat caatgagcaa gagtcccaga tattcttaga ttgtattaat
47761  ggccctcaaa ttctagactc agaaacaccc acaaagagaa gcaacaatca gaaattgcat
47821  gctattaata ttttattggt atactgaaga aagagttatg accacattat ttcaatgtca
47881  tggctttctg aaggaagtta tcttcttatt tattttctga aacaaaaaaa aaaaccaagg
47941  aaatttatac acaacataca acatggcaaa acttatctaa ttccttgatg atattactga
48001  agtaaattat tgctttcaag attaagaaaa cagatttttt tatggtgttt atgttttggt
48061  tactgttcaa aacattcaag gttatattta tgaaaatgac tgcaacttga acctgtagta
48121  gtgaaaaact aatacaagcc tacatatcaa ttaacagtgg ttgactgagg aaatcatggt
48181  acagtcacac aagagaatag tatacaattg taacaacaaa agaaaaatgt acaaataaat
48241  caccaagaag atctataggt ctccgtgaaa tgataaagtt gtctaagaca tgtattattt
48301  gaaataagca aatacagatc tgcatataaa ataatatgtc atttgtggga aaaataatga
48361  cgtatgctta tatacatata aatatttcca ggaggatgtt caaaaaatta agggtggtga
48421  cttttggtga agggtgtggg taagtaggat aaggcgtcac tttaagattt agcttcatac
48481  cattctgttc agttagattt ttttttcctt gagcatgtac taatttcaaa ataagtattt
48541  taaaaattaa ataagatata agagcatcaa gttcaagtat catttagcag ggacactgga
48601  cctgagaggc tagtgtgagg caggactgag caaagagtcc tctggcggaa acctgagaga
48661  aacctgagat ggagagtcct tgggtttgag aaggcagact caaagacagg aggcagagca
48721  ccaaagggag agcacacccc acttcctcat taccaactca ttttaatgag acaagtcatc
48781  ttaagaaaaa agttggggag gtcagtattt caaaagataa gaattctttg aaccacttgt
48841  taaaaataca aggacttgca accttatcca agaatatctg aatacaaatc tttagaaatg
48901  gggtccagga ttatgtaatt tcaacatatt tccaattgat ttgtgaacac gataaagttt
48961  gggaatggta gcagtttggg catttaatgg agaatgaact ggaatcatac ctgccactga
49021  attctagatt accagagtgg ttgctcctgg ggatgtcttg ctggtctgtc cctcYggaag
49081  aatgatgatg cttcctgcag gctgacagaa ggaaatcggg gtagagagag ttgacggtgt
49141  cctcgtYggg gtggtcgttg ctgattttga aaaggaggtS ggggaggatg gcggtgatgg
49201  cctcctggtt ttggtggtct ctgctgagga ccatcatctt ggttaccact atcaccaggg
49261  ggtctaggta ggagtccttc aggaggaggt ctctggggtc cctgatctgg tctctgactt
49321  gagtcctcta catctgtgtg agtaattaat ggacaagaat gcatgagctc agtgaagaaa
49381  aactctcctc tctttatact tctctcacct taccacacag ccctgttctc cccaaacctg
49441  ctgcttgact gtcaacttct tttgtgccca ctgttttttt tttcttttta tgcagctctc
49501  aacttaatcc atgtaagagt gatatggaaa gtctcattta tttgctcctg tgacaagtat
49561  gtttccttaa atgaaaagag gggttcttgc atatcttaga gttgccagag gatgtctcca
49621  aggacagaaa aaaaaaaaaa agcaaaagca ttatagcttt cagttatgtt actggttatt
49681  ttttattttt attttttggt tgctgtaatg acttacaaac tgtgactcca tctctgggca
49741  cctaagccct atgtggaaac tagagctgat ttcccaggct cctggaacca agtagagaaa
49801  tactaagaga cagaagagaa agacaatgtg aaagcctgca catgatcact aaacagctgt
49861  ggcaactatt tatcatctgt agtgacacag ctgagtgggt caacacagag aaaagacatt
49921  catgcagtga tttttttgtt ttgttttgtg ttactccctg aagccacata accgtgcggg
49981  ggaacactgg gggaagaaag aagcagttaa ggatggagga gtcagaatga gaacacttct
50041  catttgttca tcatctctca gcagtcattc cagcctggga aaaaagcctg cccactccta
50101  ttgtcctcaa agccaggcat ctctgtattc aagttggtgg cctggctcat ggtccccaga
50161  atcaaagatg ggtaagaatt gcctatatat ttaggggcac taatattaat caatgcctga
50221  cccctcttac cacctcctcc caaaaaaatg atgagaagaa gacactgaag gaaactaaaa
50281  agaaattcta gtggaaaaag Raagaaaaga attggattga ggatcattgg gatttacctg
50341  gtatggtgaa agtaaagtct tcatagttca catctaggaa aagaagcaca ggatgaagtg
50401  aacattacct cataaatctt tcagggctca tagtggtcta tagggaaagg ggtcttctgg
50461  ccacaccctg tgcatcccct aagttacctc atcaaccatg ttctgtgaag ctgctggaag
50521  gggaggaaga tgttagggga ggcaggattg ttactatcgc tgagcatcaa ccacgaattc
50581  aatagaagag tccctttttg tcatccttag gatccctcaa accctaatgc taatttaggc
50641  acacttctct gggtattttc atgtcatatt ttagatcttt accttcttcc ttctttaaat
50701  attttgatca tattcacaag cttgcccaag aggagcaacc aggagtaaat atttggatat
50761  aatcttacac atRctgcctg caacatattg aaattttaaa tgtgattgga agaaaaatgg
50821  gaatttgggg acctgatatt tctataccac tatgtagaaa gacacaaatg tattatccag
50881  tcctctatgt ctgacagtac tctgatgttt gtttatctct ttcattgatt ctgcatcccc
50941  tgtacagcaa ggacaccatc atcgctgtcc actgagatta ctgtagcatc ttcatagctg
51001  gacttccctg gatcagtctt gcattctcca ctgcatcctt cacagcacag ccaggtcttt
51061  ctaaaaatac aaatcttact ttgtcattcc tctaattgaa attctctatt gggttccaag
51121  tgtatatcaa ataaagtata aggccttggc atggaatgag ggcacaacag atgtcatcct
51181  tggaatgaga gctccaaagc cctacccttc ttctcaacct ccttcctccc ctcctgctct
51241  tcYgctgcag tggtgtaggg tgaacccctg aggccctaga agagtcatgg cccctcatcc
51301  cactccccat ctcctttttt aaaaaaataa tttttttacc attatctgtg ctctgagctg
51361  agctcagagc cagaaggacc actgagagca ggaccagcag catcttgaag gaggctctgg
51421  agttgctccc aactctgcgt tgagagaaac atggcagctc cctttataaa catgagcaga
51481  acaatggcac ctttgagctc catactgggt ggacctcacc acctgaaagR ttaggttttg
51541  ctttcctctc ataaagttga atgcgtctct tattactttt tgggatttta gcccagcagg
51601  atgtggtggt aggatgtggt taggtacaaa ctgtgacttg agcatagttt ctgaagaaac
51661  agtatccaag caatcagcac aaatactaca attgaagttt ggtcatcatt tgattttcaa
51721  ttgggttaga cagttagtct ttccccttca ttggttgtct gtgtgcacac gtgcttacgt
51781  ttgtatatgt ttgtcagcag tgatcttata gccagaagtg aaaatgatta atatctctag
51841  ctattagtgt agcataaatt ccatttttat agcatatata cctatatgga tgatatgtag
51901  cttaatttca taataatata gagatcattt ctgtttaatg ttgagttgtg tgaggacaac
51961  acacacttgg gcacacgtgg atgacagaag ttttaccctc aggctactct ggcccaaaga
52021  gattaagatt ctcctaatct agcattaacc agagatactt tattgttgta gaacacaggt
52081  attaaaacat tctatcttta ttaataYcct ggaggttagt aaggcaaaat tggccacaga
52141  aagcaccatt agatttctgc atcagaacag tgtgaataca cagttccttg aacaagccat
52201  gctattttat gtccctaaga cttcttccat gatctttctc ttttctagaa tgttctatat
52261  gattcagttt aggtcttact tcttgtacca aatattttaa tcacactggt tattgagtgg
52321  acacaataat acaatctaat tagatagtat gatagcctgt tgaaagcctc aagtattaca
52381  ttgattttct attttttaat tagtttccat tctatttgct tctcagattg cctttaccaa
52441  agattatttt ctctgcaata ttcttatact cattcttctt cactcttgtg ctgaacctta
52501  cttgttgata tgttatattg tgaatcaatc ctccctatgt ctatgtttta tatcttactg
52561  ctttcttcat gcgttctggg agaaatcttt atttgatttt ctaaacaagg ttctgtactt
52621  ctctttgctt gctttcttct tttttgtttt taaaatttgg caccaaggtt ttgtgtttct
52681  gaaaagtttg gtagggtcct gagttggtag cttaatcttc acctgctgat ttttctgtcc
52741  tatctagtag ttttctgctt atattttcat ggaaccccta gatcaatcag tatcaccagc
52801  tgccactttt cattacaggc Rttgtatagg tctcgtatta ttgttacatt tttgtacagg
52861  gctggtgata atttgaggat agcttggttg gaagtatgac agggtcgggg gaatgtgact
52921  ggaaatctta aagacaggcc tcttgaccat tgtggataag tggatcctgt gctgtgcaga
52981  tgaaggtctg gtctggcaat tctgaagggt tggaggtaat attacaggaa gattttctaa
53041  tcaatggtgt ttgttatgga taactttact ttggcaaagt gaccaaaaaa taaatatttt
53101  ctctggcata attatggaca tattttgaag gtgaaatata ttatgagtta gcaattcttc
53161  tctatgtggg aagcagaaga gttctagctg tgctttaggg ttttatgggt gagagagagt
53221  agttgtttgg gcctacagac ccagctgtgt tactaggatt gctgcctctg atttcaccag
53281  acactgttta acatgggaag tggagataag tagttctgcc taggtggctt atggtagacc
53341  Rtcaggtagg aaaggaaggt cagcgagaag gagcaSaggg gcgtgccatg cagacatgat
53401  tcactgtgtg ggtggctgag gaaactgcag tcctggtgga aggcaggctt ctccttggct
53461  ggtgagtgca ggaagacaac aggtgatgcc tgcaaatatc actcagctca gacccttacc
53521  ctgacattct ccagtgtaag caatgagaga aagagattaa tgtatcagga aataataaac
53581  atcagctatg atctcaccac acacaaccac aacagaggtg acggaggagc tgaaacagac
53641  actgtcagtg agtgagggtg gggtcaaatc gagaagcagg actgcagcag gagcttaggc
53701  acttgtaggt gagtggacac tctcagcaat ccacttgcct tttacaacag atgaaaactt
53761  aacatacccc taatataatt cttgatttct acttcctaaa acctgctctc cttcatacta
53821  atttgttatg gctaccctaa aaaaatgccc cacaagtggc ttaaacaaca gaaatttact
53881  ttctcatagt tctggagctt gaagtacaag attaaggtgt caacaggttt ggtgtcttct
53941  gaggccctgt ctccttcact tctagatggc cggttcttgc tgtatcttca catggggttt
54001  tatctgtaga cgtgcatccc tggtacccct ctgtggtctg tgctaaYgtt cttttcttat
54061  gaggacaggt gtcagatggg attagcacca accctatcag cttcatttta acctaatcac
54121  ctctttaaag gccatgctgc aaatagagtc acattcagtg gtatcggggg taagggattc
54181  aacatgagag tttggaggga acacaattta gctgataaca cctcacaatc ttttctcagt
54241  tcatgacatt tgtttcactc aaccattcaa aaacataagt gtcactcttg attgtttagt
54301  ctcctccgtg tactgccctt agcaaatcca attggttcta ccttcaaaag atatcccata
54361  tatgatcagt ttcataatct ctatcagaag gYcctgagtc aagccatgat tctccctcct
54421  ggaggacttt caacagtctc cagctgatct tggtgacatt cctgcccctc accacatccc
54481  aWgcagttct ccttacaaca gccagagtga tcttttaaaa atagaattta gaacatcagc
54541  aagatggctg actagagttc cctggcactc atttccccac aaaaagaaat caagaaaaca
54601  aacaatttta tgtcagccag tgtgatttaa aaagtatgct ggagaccacc aggggaacag
54661  aaacacctct gtgaagtaca gaaactcaat atagcatcct gcctctgaca tactctctct
54721  tcagtcaagg ttggctcagg gtcatcagta actccattct acaggaaaaa gtaagctgta
54781  gttccccagt ggtccacatt gccataaaaa atgtcaacaa tctttgcccc actgttctca
54841  ctggcacaaa aaccagtctg gagagcagtt gggatttcca aactgcattg ccttacatag
54901  taagagacca ccttgagcac cctctgcccc tgcaatgtaa ggtgctatgg ctcagtgcca
54961  tctccaaact gaacctacat agagagtgtg tcttgctttg agcatcagta gcacctggct
55021  ctctatcact tgaggcccca ccatcattcc aacacattca tacaggtatc tgcagcacca
55081  tgaccctagc ttcccagagc ctagccagat aaactaatca agaccccggc atccgaaccc
55141  atgtggtgca ccaccaatcc agggaagaag tgaacagcag ggaagccacc catctgccta
55201  ctagccacga cacccacatg cacccacacc tcacaaccag ctagtctgct gagcctgtgc
55261  atgctggtgc ccagcctgaa aactggtcct gtggtgggcc cacccccagg actacagcct
55321  gcttgggcca caacaactct gagtttgtgc atggtctaat aattggtcct gcagtgaccc
55381  tgcctcctca gatgggactc tgcagagatg gatggccctg ctacacctgt gagcactgac
55441  agcaacccgc ccacccagca ggaagacMgc agtgcatgta agtgcacaca ccttgagaaa
55501  aggctcttcc cactgctgct ggtggcacag ttgctgctgt caccaccggg ggctgcagca
55561  gtgaaatgcc agttggaccc agcaaagtgg caggatcctc agcattctag catatgcagt
55621  gttctgcacc tcaggcactg gaaaggctgt gaaccagaca tagggagcca aagcacatgc
55681  tttccagaac cagagagctg cctccctgtg gctgctgaca cagacagcaa tgtcaccccc
55741  gcaacacagc agcagagatg ctgcacactt gcatgcaacc tggggacagg ccctctccat
55801  ctgctgctga gtctgctgat gcagctgggg gccagagcat gtgccactgg cagtgacctg
55861  acttccacca gcagcagagc cactgtgaac ttgcacgtac cctgaggact ggcttatgct
55921  gctgccagaa gccaaagtgt gctccacatt gccagagagc tgcctgctag tggctgctgc
55981  cactgacaat aaccacaccc tgccctagca gcacagctgc agcaaatttg catgtgcctc
56041  gaggacaggc tttcttcagg tattgctcct gctattggga gatccaagga ttaccccatc
56101  caacttacca cagccaacat ccatgtacat aactaggggg actgaggaca ggcctgccaa
56161  gcctggttcc atcatcccag tgcccaggca cactacccag gggtgtggca atcatcctgc
56221  tttgtccacc accactggca tttgcacatt cctttaggag gactgagaat gggcccaacc
56281  agcctgacag tagcactgta gccagaaaca atatgcatgc aacatctgaa ggtctggaga
56341  ctggccccca gcccatcaca accacctcta acaccagagc ttgcaacttg agaatctgag
56401  ggttgtccaa ccaagctaca gaaattgccc atggcaagca cattcccaga ggcctaaagg
56461  cccacccacc ctttcagcct accattgcaa ctgctagtac ccaggtaagc tacttaaagc
56521  ccccagaatt gttctgctta aagctgctaa aactggtaca aacatatgtt gcctaggtgc
56581  caaatatagg cactctcagc ccactgttac ccctactggg gcccaagaac tggcccacct
56641  aaaatctctg tccccaggaa aacctcacta cagcctccaa tcacaactac agcctaagcc
56701  actgaggaag tcacagacac cacttatgct gttaatagct gaaggaataa tataagacca
56761  ccttactgta cacacacaga atcaaagcca aagtgtccta ctgaatcaac accacagata
56821  catcttcaga aagaaaagtt aatgttttgg atctatgtca ctgcccaaat ctcatgttga
56881  attataatct ccaatgttgg gggaggggct tcattggagg taattggatc atgggggtgg
56941  atccttcatg aatggtttag caccatcccc tgcgtactat tcttatgacR atgagtgagt
57001  tctcatgaga tttgcttgtt taaaagtgtg tgtcacctcc cccccaccca gtccttctcc
57061  tgctatgtaa gatgcctgct cctgctttgc catttgccat gagcaaaagc tccctgaggc
57121  ctccccagaa gcagatgtca ctatgcttcc tgtacaacct atgaaactgt gagccaatta
57181  aacctctttt cttcataaat tacccactct caggtacttc tttatagcaa tgtgagaatg
57241  aactgataca gaaaattggt accgaagagt agggcattgc tataaagata ctaaaaatgt
57301  ggaagcgatt ttggagatgg gtaatgagca gaggttagaa gagtttggag gactcagaag
57361  acaggaagat aagggaaaat ttggaacttc ctaaaaactt gtaaattgtg accaaaatgc
57421  taatactgat atggaaaatg aagtctgggc tgaggagatc tcagataaaa ataaggaact
57481  tattgggaac aactggagca gtcacttttg ttatgcttta gcacagagcc tgggtgcatt
57541  gttcccctcc tttagggact tgtggaactt tgaacttgtg agtgatgatt tagggtatct
57601  ggtggaagaa atttctaagc agcaaagtgt tcaaaatatg acctggctgc ttctaacaac
57661  ctaagctcat atgcctaagc aaagaaatga gctaaaactc aaatttgtat tWaaaaggga
57721  agcagagtgt aaaagtttgg aaaacttgca gcctggccat gtggtagaaa aaaaattccc
57781  attttctaga gaagaattta ccctagctgc agaaatttgc atgagtaaag aataaccaaa
57841  tgttaacagc caagacaatg ggagaaagac attgaaggca tttcagagac tttcgtagcc
57901  acacccacat cacaggcctg gagtcctagg agggcagaat ggtttcctgg tccaggcgca
57961  gggccccact gacctgcaca gcctccaaac actgtttcct gcatcccagc tgctccagct
58021  ccagctgtgg ctcaaagggg tccagctgca acttgggctg ctgcttcaga ggatgccagc
58081  cataagcttt ggcagcttcc atgtggtgtt aagtctgtga gtgtgcagag tgcaagagtt
58141  gaggtttggg agcctctctt ttgatttcag aggatctaca gaaaagccta aatgtccagg
58201  cagaagcctg ctgcagaggc agtgccctca cagagaacct ctactagggc agtgcaaagg
56261  gaaaatgtgg agttggaacc cctacacaga gtccccactg gggcactgcc tagtggagct
58321  gtgagaagag agctgtcatc atgcagaccc cagaatgaca gatcctctgg cagcttgcac
58381  catgcacctg gaaaagctgc aggcactcaa tgtcagccct tgagagaagc tgtgggaaat
58441  gaaccctgca aagctacagg ggtggaactg aaaaggcctt gggaacccac cccttccatc
58501  agtatgcctg gttgtgaaac atagaaagtc aaatgaggtt attttgcagc tttaagattt
58561  aatgacttcc ctgctggttt cagatttgca tggggtctgt agcccctttc tttttacagc
58621  tttctctttt ttggaacagg agtgtttacc caatgcttgt actccccttg tatcttggaa
58681  gtaactgttt tttgaattta caggctcata ggtgaaagag actagccttg tctgagatca
58741  gcctttggac tttcgacttt tgagctaatg ctgaaatgat ttaagacttt gggggactct
58801  tgggaaggca tgattgtatt ttgaaatgtg agaaggacat gagatttggg agtagtgatg
58861  ggctgaatga catggtttgg atctgtgtcc ccacccaaat ctcatgttga attgtaatcc
58921  ccagtgttgg ggaagactgg catgttggga ggtgactgga tcatgagggc agattcttca
58981  taactggttt agtactattc tctcactgct gttctcatga tggtgagtaa gttctcatga
59041  gatcttgttg tttaaaagtg tgttgcatct cgcccccact tggtcctgat cctgccatgt
59101  aagactcctg ctccagcttt gccttttgcc acaagtaaaa gctccttaag gtttccccag
59161  aagcatatgc ttctatgctt cctgtacagc ctgcggaacc atgagccaat taaacctttt
59221  tttgaaaaat aaattactca ctctcaggta tttctttata gcaatgtgag aatggactga
59281  tacaaaaatc ttcccctatg aaagccaatt taaaaaacag aagaagcaac tatttcacca
59341  gatgcgcaga catcaatgta aggacacagg aaacatgaaa aagcaagaaa atatgacacc
59401  tccaaatcaa cacaatactc ctccagcagt agattccaat gaaaaagaaa tttatgtaat
59461  gttggggaaa atatttaaaa aatgatacta atggagctca gtgagataga agagaacaca
59521  ggtaaaaaat agaaagaaac cagaaaacca tttcaggata ttaaagagaa attcaacaat
59581  gaaatggata tcataaaaaa gaatcaaaca gaaacgctgg agataaataa atcaataaat
59641  gaaagaaaaa tacatctgaa agcttcRaaa atagaatagg tcaRtgagaa gaaagaatat
59701  cagaacttga aaacaaatat tttaaataac ccagtcagac aaaaaaagag ggataaaatg
59761  tgaaaaagat gaacaaatcc tacatgacat atgggatgcc ataaagcaac caaatttttg
59821  aatttgggtt tttcccaaag gcaaaatgaa aggcaaaaac ataaaaaacc tatataatga
59881  aataatagaa gaaaacctct caagtctagc aagagagtta aacacctaga tacaggaagc
59941  ttagatagat acaacccaaa aatattttct tcatgtcaca ttatactcaa actgtcaaaa
60001  gtcaaagaga aaaacactat agaagtggca gagcaatatg gtggaataga aggctccacc
60061  aatcatccca cccacaggaa caccaaattt aacaactgcc tacataatca aagcacttcc
60121  ataagaacca aaaatcaggt gatcactcaa agtacctgct tttaacttca tattactgaa
60181  agaggtactg aagagggtag gaaagacagt cttgaattgc catcgccacc cttgtcttaa
60241  cccagcagca actacataga atggagaatc tgtagacttg gggagggaaa gtgtggtgat
60301  tttgaaactt cgcattgaaa tcagtgctgc cttgtgacaa tggaaggcaa aaccggggtg
60361  aactcagctg atgtccaccc acagagggag catatagacc agccctagcc agaagagaac
60421  tgtacattcc agcagccaga acttgagttc tggcaagcct tgtcactgta ggctaaagta
60481  gtctgggtct ctaaataaat ttgaaaggca gtctaggcca caaggactac aaatcctagg
60541  tgagtcctag tgctcagctg ggctcagggc aagtagactg gtgggggcgg tgggtacatg
60601  acctactgaa acatcagctg gggtggctaa agcagtactt gggccatccc tccctcaacc
60661  cccagctaca cagcttatgg ctccaaaaga gaccacttcc ttccacatga ggagaggaga
60721  aggaagagtg aggaggactt tgtctggcat cttggatacc agcttagcct cagtaggata
60781  gagctctggt cagagtcatg aagccctgat ttcagactct agctcccaga tgacatttct
60841  agacacacac tgggctggaa ggaaacctac tgcattgaaa ggaagagcca agtaatgaaa
60901  gaaccaatca cctgctgatt aaagagcact tgggccttga ataacaagcg gtgatatcca
60961  ggtagtatgc tgtgggcctt gagtgagact ctgagatgtg ctggtttcca gtgagaccca
61021  acacattcct agctgtggtg ggtatggtca gagattcctc ctgcctgaga aaagcagaga
61081  gaaaaagtaa aggggacttt gtcttgaacc ttaggtacca ggtcagccac agagaagtat
61141  aacaccaagt gggctcttga ggtccccaat tccaggccct ggctcttgga cagcatttat
61201  ggacctgctc tggtccagac ggggagccca ccactataaa aggtgagtcc tagatctggc
61261  agcattattc accacaggct gaatgaagag cccttggtcc ttaagtgagc atcagtggta
61321  gcctgagaat attcccatag gcttgtggtg atggtggcca gggggcaagg tgcctgcata
61381  tgaaaaggga agattagaat gggaagaatt gcatctactg gtttgagtgc cagcttaaca
61441  gcagtacaat agaacacaag gtaaatttcc aaagttttgg actctggtcc gtggctcctg
61501  gacagcattt ttggacctgc ctggagctaa gggatcttgc tgctatgaag agaagcacat
61561  aagcctatcc gtaagcccca aagcagtact aagaaggaag attattgcta tatgtgccta
61621  catcaaaaaa taaaaaaact tcacataaac aatctaatga tgcatcttaa agaactagaa
61681  aagcaagagc aaaccaaacc cgaaattagt agaaaaaaga aataaagact agagcataaa
61741  taaatgaaat taaaattaaa aaatacaaca gaagaatgaa acaaaaagtt tgttttttga
61801  gaagatgaac aaaattgaca aaccttttga cagactaacc aggaaaacaa gagagaaaac
61861  ccaaataaat aaaattagag atgaaaaagg agatattaca acctacacca cagaaattca
61921  ggggattatt agagactact gtgagcaact atatgctaat aaattgaaga aactgataaa
61981  ttcctagaca aatgtaacct gccaagattg aactatacag aaatccaaaa acctgaatag
62041  acMaataaca agtgatgaga tcaaagccat aataaaaact gtcctagcaa acaaaagacc
62101  aggacctgat gacttcaatg ctaaatttta ccaaatatct aaagaagaac taatactgat
62161  ccttctcaaa ctattctgaa aaatagaaag ggaaggaata ctttcaaact catttggtga
62221  ggtgaatatt accctaatat gaaaactaga caaagacaca ttaaaaaaaa tacaggccaa
62281  tattttggat gaacattgat acaaaaattc tcaacaaaat gctagcaaac agaatgcaac
62341  agcccattaa aaatacaatt catcattact atgtggcatt tatcccatgg atgcaagaat
62401  aattcaacat gtgcaaacca atgaatgttg ataaatcata taaacagaag gaaggacaaa
62461  actaatatgc attgcaatag ataatgaaaa tcatttgata aaattcaaca ttccatcatg
62521  ataaaaatca tcaaaaactg ggcatagaag gaaaatgcat caacattatt aaaaccatat
62581  atgacagacc cacacctaat atcatactga atggggaaaa actgaaagct ttcctctaag
62641  atctggaaca tgacaaggat gctcactgtc atcattgtta ttcagcatag tactggaagt
62701  tctaactaca gcaatcagat gaaagaaaag agaacggaag ggaagggaag ggaagggaag
62761  ggaaagggag ggaaggggag ggaaggggag gggagggcag gggagggcag gggaggggag
62821  gggaggggag gggaggggag gggagggaaa agaaaagaga gcatccaaaa tggaaaggaa
62881  ggagtcaaat tatctttgtt tgcagatgat qaaactttat gtttggaaaa aaataaagac
62941  tccacaaaaa tgtattagaa ctgatagaaa cattcagtaa aatttcagga tacaaaatca
63001  acatacaaaa ttcagtaaca tttttatatg cccacaggga acagtctgaa aaagatcaag
63061  aaagtattct tatttacaat agctacaagt aagataaaat acctaggaat taaccaaaga
63121  agtgaaagat gaaaagtata aaagtataaa acattggtgc aagaaattga agataacaca
63181  ctaaaatgga aagacattct atgttcacgg attggaagaa tcagttttgt taaaatgtcc
63241  atactaccca aagcactctg cagattcaat gcaatcccta tcaaagtatc aaYgacattc
63301  ttcacagaaa cagaaaatac aattctaaaa ttcatatggt accacaaaag actcagaata
63361  gccaaagctg tcctaagcaa aaagaacaaa tttggaggaa tcacattacc tgactttaaa
63421  ttgtcttaaa tactacagaa ctatggtaac caaaatggca tgacactggc atagaaacag
63481  acatKtttgg actgtgagcc tggcctctat gaaccatcgt ttcagctcct gattggtcca
63541  gagccaaggc cctgggccaa gctgagtcac aagttctcca agacagccca tggactaagt
63601  gcattccttc cccttcccag tccataaaca ccctggaccc cagcctcata gagggcaatc
63661  catttgggtc cctctttccg ctggcagaga gctttcttct tttgtttgtt aaacttatgc
63721  tttaacctca cattgtgtct gtgcttctca atcatcttgg acgtaggaca aagaactYca
63781  gatattatct cagacaataa gagactgcta tctgggtccg ttggcaaaac tacaacatta
63841  caacaagaaa actttgggga aactctccag aacaccatag tgggcaaaga tttcttgagt
63901  aatacctcac aagcactggc aactaaagca aaattaaaat taaaaaaaaa cttcaaagat
63961  aaaaattatt aaaaagagaa aagcatccag tcatatgtaa gggaactatc accagatcaa
64021  aagagaattt atcaacagaa agtttatagg ctaggagaga atagtatgga atattcaaag
64081  ttctggaaga aacactttca gtcatacata ctatactcag aaatgttatc cttcataaat
64141  aaagaggaaa taaaatcttt cacagataag caaaagcttt agactaacct tacaacaagt
64201  gctcaaggga gttctacatc tRgaagcaaa cagatgataa tcactatttt gaaaatacat
64261  aaatactcac tgttagagaa gacacacaaa gaataaagag aatcaagcct tattatttca
64321  gaaaactaaa agaaatgata aacaataaga gaggaagaaa ggaagaaagg gacatataaa
64381  acaaccagga aacaatgagc aatatagaaa aaccaaaacc tcacatgtga ataataaccc
64441  tgaggtaaac aaattaaatt ccccatttaa aagatatata agtcatataa aagggaacct
64501  ccattagatt aacaacgaat ttgtcagaag aaaccttaca ggccaggaga gaatgagatg
64561  acatattcaa agtattgaaa caaaaaaaat cttatcagcc aagaatacta tttccagcaa
64621  agctattctt caaaaatgaa gtagaaataa tggcgttcct aaacaagaaa aactggaaat
64681  tcatcactac tacactggtc ctacaagaaa tcctgaagga aattctatac tttaaagcaa
64741  aaagacaata tggtccatca caaaaacaca cacaagtata aaactcactg gtaaaccaaa
64801  tacacaaatg aaaaagagaa aggattcaaa ttttaccact acataaaatc aacaaatgac
64861  aattataaac aataaaaaat atagaaaggt aaaaggacat atgaaataac cagaaaacaa
64921  ttaataaaat gacaagaata aatcctccgg tagtaataag cttgatggta aatgaactaa
64981  aattttcagt ttaaagatat agactggctt aatggatttt aaaaagcgtg acacaagtat
65041  atgctgccaa taataaagta aatttctctg taaagacaca tggacttaaa gtgaagggat
65101  gaaaaatgat attctatgca aatagaaaca aaaagtgaga agtactatac ttgtatcaga
65161  taaaacagat tttaattttt aatttgcaaa cagcaaaaag acacaaaaaa ggtcatcatg
65221  taagataaag ggatcaattc agcaagaggg ataatacaat tctaaatatt caaacaacac
65281  tggagcaccc agatatataa ggcaaatatt attagacata aagggggaga tagattccaa
65341  cagaataata gtgagggact tcaatgcccc attctcagcc ttagacacat aatccagagt
65401  gaaaattaag aaataaacat tggattttaa ctgcacttta gacatgtctc aaaaaaaagt
65461  atacgaatgt ccagtaggta tataaaaaat gctcaacatc actcatcatc agggaaatgc
65521  aaattaaaaa cacaatatca tccttctcca gttagaatgg gtattattaa aaagaaaaaa
65581  aaaacagatg ctggtgagga ttcaaggaaa aactcttaca taccgttggt gggaatgtaa
65641  attagtacag tcactatgga aacaatatgg agatttctca aaataactaa aaatggaatg
65701  accatatgat ccagcaattt cRcttctggc tatttatcca aagaaacaga aatgaggaca
65761  tcaaagggat acctatattc aaatgttgac tgcagcacta ttcaccatag caaagatatg
65821  gaatcaacct aagtgtccgt cagtggacaa atggattaaa aaaaaacagt ggtatatata
65881  cacaatgtaa tactattcag ccataaaaaa agaataaaat catgtaattt gtagcaacat
65941  ggatggaact ggagggcatt atgttaaggg aaataagcca ggcacagaaa aacaaacagt
66001  gcatgttctc acttatgttg atcacacaga ggtagagaat agaataatgg ctacaagaga
66061  tggaccagtg tttgtgaggc aggaggacaa gtatgaagag aggttcatta atgggttcaa
66121  acatacagtt agaaggaata aattcaaata tttgataaca gattagggtg actatgttta
66181  acaatatttc aaaatagcta gaagaaagga cttgaacagt tccccaaaca tagaaatgat
66241  aaatactcaa gatgacagat accctaactt gatcattata cattctatat gcttataaca
66301  aaatatcatg tgtcYcataa atatgtacaa acattatgta tcaattttta aaaatcagga
66361  ttgatagctt tactgctgaa ttctaccaga catttaaaga ggaattgata ctaattatcc
66421  tgaaacaatt ccagatattg cagaggaagt aatacttcca aacgcatttt acaaagccag
66481  cattaccctg attccaaaac caaagataca acaaataaaa aagccacaag ccaacatccc
66541  tgatgaatat caatgcaaaa attcaacaag attttagcaa agtgaacgca gcagcacatg
66601  aaaacgatca ttcactatga tcaagtgggt tttattcaac atatacaaat caataaatgt
66661  gatgcaccct attaacaaaa tgaaggacaa aggccatatg atcatctcta tagacacaga
66721  gaaagtattt tataaaattc aatatccctg cataagaaaa gctcacaaga aattagacac
66781  acaagatatg tacctcaaca caaaaaggcc atatatgaca aacccacaRg taacatcatg
66841  cagaataagg aaaggtgaaa gatttcctct aagattagga taaagataag gatgttcact
66901  ttcatcactc ctattcaaca tagtattaaa agtattagct agatatacta ggcaagagat
66961  agaaaYtaaa Maaattgaaa ttgaatagaa ggaagtcaat tgttcatctt ttYggatgac
67021  atgatcttat ttatagaaaa ctctaaagac ttcaacaaaa aactgttaga ataaatctgg
67081  taaagttgca gaatagaaaa tcaatgtaca aaaattagta gcatttctgt atgctaatgg
67141  tgaactatat gaaaaataat tcagaaaaca atttcattta caataaccaa aaaagaataa
67201  aatacttagg aataaactta accaaggaga taaaagatct ctaatactgc aaactatgaa
67261  acattgataa aagaaattga aaaagatgca aataaatgaa aagatactct atgtttatgg
67321  attggaacaa ttaatattgt ggaagtggcc atactaatca aaatgatctt cagatttaat
67381  gcaatcccaa acaaaatacc aatcagcata cttcacagaa ataaaaaaag cacacacata
67441  ttttccgtct ttgagtaatg tatgctctga aaggggaaaa aggcattgag atacaaaagc
67501  acattataag atagaaagct ctatgtgcca tttcctgtca tttaccttga tattagatcc
67561  aggaaactct ctctattttt aggccaaatt attagcaaac ttaattgccc ttcgccatgt
67621  aatttaacat aaaacacagg ttctgggaat taagatgtaa acatctttgt gaggcctata
67681  ttttcctcct gcagagagaa caggtaatct tacatctctc aaatttgttt cagttgtgta
67741  tggagacagc tatccctgat ttggtaatgt ttaaactaga cttttgcaaa aggatatgta
67801  tgagtttata taaaatttga tagtattgca tgtatttata ctgtgcaata tgtatatgca
67861  atagtttaag tgacaaaact aagtggtaca aatttacctt gaggttggct gcatttgtaa
67921  gttggggcag tcacttcttt ggagtgttgt gcagacttgg gaatgagaag ctgtagattt
67981  cattctaact ctgccactta cagcaggaca accacttgca aagcccagct ttctctatta
68041  aaataagacc ctaaatcttc ccaacaccaa taatgaagat gacattctca gatcaggaaa
68101  atgaagggtt cttcagaagg atcatcactg tgtcaggggc agcaactgga gatctgaaca
68161  aaatagctac ctgggcatcc aatccctcta ctgtgaaatg catgattctc cagacctttt
68221  ccaacacgca tagagctttc tgatcgtttt agtgcctact cttaagtatg aattgaatca
68281  aaggttgacc agacaattga agaaattctc catcaaatga gactcaaaga atcagaacaa
68341  agtgtaattc tgaaggaaaa gaggtaagat aaacagcaga agaaacgttt acaagactat
68401  aattaatata ttctgatata accataaaac ataaaaggat gctatcaaat agaagattga
68461  ccagagaaca caaaagaata tgagcaattg taaattaaat tgataagttc aataaaatgt
68521  aaagttaagg aaatccccta tgaggtaggg caaaaaggca aagatatggt ccataatgtg
68581  aagacaatta aaaaattcac ccaacatgaa ttttagaaaa agaaaacaga gtatatattg
68641  ggaaagacat tatcaattaa atactgccat acattaccca gaacttatgg ataattctct
68701  ccataatgaa aaaagccatt gattatctaa aagaccagat ttaacatttt caggccccag
68761  tgcaggaaga caaatggagg ctcacatact ctatctctga ataaccatac attataaagt
68821  aaaatcgacc ctaggttcag gttaggtttc catccaagtc ctgaagcctg tcccttggag
68881  actgcccttc taacctggcc tagagacaga agaatcatgt gtttccctgc accaacactg
68941  actgatcctg caagctgaga ggtgggaaaa tacggagtgg cagggctgtc tgctggagca
69001  gtgggcagga gctgagatga ggcccagcac tgacctggcc aagagacaga agaatcatgt
69061  gtttccctgc accaaccctg actgatcctg caagctgaga ggtgggaaag tatggagagc
69121  ctctaatgag ttttcagttc agcaaatcta gttaccagtc cccagatttc tgtttgattt
69181  ttcaaaatta tttcaatctc tgttaaaatt ctctgataaa tcaataaatt gctttcctat
69241  gctatcttgc agatatctga tttccttaaa attgttattt tgaattcttg atcagagagc
69301  taaccaactg tcatgtcatt ggggtcagta actagtttgg tacctttgtc cttttgggaa
69361  ggccatggct ctttgcttgc tgttgtttcc tgtgggtata cttctatgtc tttccataga
69421  agggttatta attaattcca gtttttcagg tctgggttgt gtttgttgtt attgagtcta
69481  tttgcttggc aaatcctcac tgctgggctg ttgcctccct tttgactcta ggtggtgtct
69541  caacctcact tttaccttgt ctttagtaaa cggttggagt attaactgtc cgaaatggga
69601  aatgtcccaa aggaattacc ctggaagtat gagaaacttg gctaggggtt atggtctggt
69661  gacctaagga atgtacctcc taaagcatgg tgctattgaa catctaccct gatttggctt
69721  ctcctttggc caagttatga acagtgtcca gaactggaga tgatcttccc tcctccctat
69781  tttgtctctt gctctcttca ggaatattcc tccctgagga cagagaatat tcatcttccc
69841  taagggagtc ataatgcatc tcatgggcta aggaagaaca agtttcttgc cagggaatcc
69901  aaggtggtaa ataatctgtt tgagaacatc aatttcactt tattcactct aaaatccatg
69961  aattggggga gattctctgc atgtgtggtg ccaggaagaa taggaaggag ggacatcatg
70021  gaggtggaag tcccatctcc tactgtcttc ttgatgattt tccacttctc tttggccctg
70081  agaaccatct cattttcata cttgagctat gtatttttcc tgacatagat ctcaaagctc
70141  tatatttgtt tttggtattc tgtttgttgg ggtagtaagt cagcttgcct ctacagcaac
70201  actttggaac tagtgggtag ctattttgtt gagacaacaa tatgctatct ctgaaaccac
70261  actgaccctt cagaaaattc ctccctcttc ctgacctgca agaataataa tcactgccga
70321  cactttggag ctggtctggg ttcatcccat tccatccagc actgctggca cccaWgtact
70381  tctctgatgg gtctgaggac aaacctacac agcctgtgcc aagaccattg ccagtacgta
70441  cctgcatgtg acaccttgga ttcttaggac tggcctacat aggccattgc catcaccatt
70501  accaccagca cattcaacct ggaagcctga gggttgtctt gctactgcta ttgctatcac
70561  ccatgccagg cacattgcac aaagtctcaa gaaccactca ctcaccaggc ccaccactgc
70621  aacttccagc accacaggaa actccctgga ggtacaataa tttacccaac tgaaactgtg
70681  gatattggtg tcaatgtttg ttgtctggag gctcaaagac cggcatgctc attccactgc
70741  tgccactaac atgtgttgca gactggccca tctagtaccc ctgttcccat caaaacctca
70801  caaaaacttt tactaacaac ctcagtctta gcaactgagg aaatcacaga ccactgatcc
70861  cgactagaga caaagaaacc atacagatat tatgctagtg tatacaccca gaatcaaagc
70921  taaagtgctg tatgcaacca acactacaga tatatctata gaaaaaatag tgttccccta
70981  tgaaagccaa tcaagaagag gctgttactc cagacgtgca tatactaata tagggactca
71041  ataaacatga agatgctaga aaacatgaca cccctacagg accacaatta ttcttcagca
71101  ggagattgca acaaaaataa aatctatgaa cttctaaaaa attcataaat atgatatcat
71161  agaagcttag ttaaatataa tagagcaaag ataatcaata caaaaatctg aaaaaaatca
71221  gaatatgaat aagaaattca ccagatacct agatgtcatg agaaagacca aacagatatc
71281  ttggtagtaa agaattcaat gaataaagta aaaaaaaaaa aatattttca aaagcttcaa
71341  cgatagacta ggtaaggcag aagaaaaaaa ttcaccactt gaggactaga tttctgaaat
71401  aaaccagata gaaaatataa taaatcatct aaataaagcc agtattcaat ataacatacc
71461  atgtagttac caaaggtttg attctgagta ttccagaata ctcaagaaga agagatgggc
71521  agaggcatga caaaaatttt aaactaagca catgtaccct aaaacttaaa gtataataat
71581  aataaaataa aataaaaaaa gaaaaaaaaa taatagctga aaatttgtca agtcaagtct
71641  agcaagagat ttagacattc aaatagagga cctcaatgat actcaaattg acacaacccc
71701  agaagtcctt ctccaaggca tacagtggtc aaactgtcaa aagccaaaag tcgttaattc
71761  taaaaacagt aagataaaag catcgagtca tgataatgga accctcatca gattaacagt
71821  gattttcact gtataaactg tgaaagagag ataaaatcat atatttaaag tgttcaaaga
71881  aaaaaaatct gccagccaag agcactatct tcagcacagg cgaacttcaa aatgaagtaa
71941  caataaagtt tttccctgaa aagcaaaaac agagaattca tcaccacaat gactgcccca
72001  caagatatgc ttaagggaat tctgctcctg aaagcaaaag tacaatatct accgtcatga
72061  aaacacaaaa aatttaaaat actccctagt acagcagaca aatgaagact tgaaatgatt
72121  taaattttac tattatagaa aaatactaaa ccacaataat aaaaagtgag agaaaaagaa
72181  acaaagtata tgtaaaacaa acaaaaacaa ataacaaaat gaagtaattt ctcacatatg
72241  aataataatc ttgattgaaa attgattaaa ttctccactt gaaagacata gactggctga
72301  gtgaattttt aaaaaatatc ctaactatat gctgcttaca agaaactgac ttcacctaat
72361  aagacacata tagactgaaa atgaagggat agaagaaaat atcccatgta aacagaaaca
72421  aaaacaagtc gaaataggta tattaacatc agataaaata gactctatgt caaaaacagt
72481  aatgagagaa aaagaaggtt attatttaag aatagaaaga tcaatttagc atgaggatat
72541  aacaaaacta aatatattcc cccaacacta gagtatacct agatacataa agctatcatt
72601  atgacatcta aaaggataaa tagattccaa tacaatagta gtgtaaactt taacacctga
72661  ctctcagcat taaacagatt atttagacag aaaatctaca aagaaatatt ggacttaatc
72721  ttaagaataa ggggacccaa tatacattat agacattttt atttaacagt ggcaaaacat
72781  accatttttc ttattagcct gtggaagatt ttctaataca gaccgtatgt taggccacaa
72841  aacaagtctc agtacatttt gaaaaagcag tatactttat tttcatatac cacaatggat
72901  aaaactaaaa atcaatatta agaggaactt tggaaattgt aagaatacat ggaaattaaa
72961  caacatgctc ctgagcaacc actggttcaa tgaagaaatt tagaagaaaa ttaaaatgtt
73021  tttgaaatat gacaatgtaa acacaacata cagcacaaat agtgctaaga ggggagatgc
73081  tctgaataaa ttctttcatc caaaagtaga gagatttcaa ttaaacaacc cagtgatcca
73141  cctgagggaa ctagaaaact aaaaacaaac tgaaccccaa attagtgaaa ggaaaaatat
73201  agtaaaaata agagcagaac caaacaacta gaaactaaaa aaaaacaaac ccaaactcaa
73261  tgtaatgaaa agttggttta ttgaaacaat aaataaaatt cctaagtgtc tagctaggct
73321  atactatata tgtgtgtgta tatatatata tacacacaca cacacgtata tatatatatg
73381  ctatactata tatatagtat agatattgtg tatctataca tatataaaaa tatatatata
73441  gacagaaaaa actgaatgaa acaaaatcag aaactaaaaa gaagatatta caacttatat
73501  catagaaata caactgtata ttttggacaa taatacacta atgaaaccgt atgttttgaa
73561  caagtataca ctataattga acaccatgga aaaagattaa ttccctgaca tatacaactc
73621  acacaaaatg agccaggaaa gaagggtaaa cctgaacaga ctaataatga gtaaagaaat
73681  agaatcagta ataaaaacga aatctcccaa caaagcaaag tccaggccta gatggtttca
73741  ctgatgaatt ctaccaaact tataaggaaa aaggaaaagc attcctcatc aaacaattcc
73801  aacaaattca gagaagagaa tacttcctaa ctaattttac aatgccagga ataccccaat
73861  gctaaaaaca tacacgaaca caacaacaat aactataaca aaaattagag gtcagtattt
73921  ctgatgaaca cagacacaaa aatcctcaag aaaatgttag caaaccaaat ctaagaacac
73981  atcagaaaga taattagcaa taatcaagtg gtatttatcc cagggatgca agaatggttc
74041  agcatacatg aatcgataaa tgtggtacat cacattaaca gaataaagac aaaaccaaat
74101  aagcatatga atagatgWag gaaaaagcat ttgataaaag ttagcatcca tttatgataa
74161  aagttcccaa cgaactacac aaagaaggaa tatacctcta tataatgtaa ccacatgtga
74221  cgaactcaca cctaacatac agaatgggga aaactgaaag ccatttctct aaaaactgaa
74281  ataagacacc actcagtcaa cattttactt tatgtcatgt cctagcgaga agaatcaggc
74341  aagcaaaaaa aataaaagcc atccaaattg gaaaagagga agttaaattg ttcctgtttg
74401  cagacgacat agtcttatat taggaaaaac acactggggc ctttcagagg gtagagggcg
74461  gaaggagaaa gaggatcaag aaaaataact aatggtacta ggcttgatac ctgggtgatg
74521  aaataatctg tacgacaaac ccccatgaca caagtttacc tatgtgacaa acatacagtt
74581  gtacgtctga acttagaata aaagttaaaa aaattgttaa cataaaaaaa gaaaaatcta
74641  aactccacca aaaaaattta ggactatcca ataacattgc aggataaaat atcaatatgc
74701  aaaaattagt agcatttcta tatactagca ataacccagc aggaatgaaa aatcagctat
74761  gaaattccct ttacaaagct ataaaaaatt aattactcag taatacattt aagcaagcag
74821  actgatgttt accagctata tgactgaaaa tgtcaggcaa tcatattgag cttttgtttt
74881  ttcatctgaa gagtaaacat aatgcaagga attattaaag ggctaccata ggaataaaag
74941  agctaatggt ataaaacatt attagtatta ttagtattgc atagtatcta tgcaatagtg
75001  taagtcagaa actgaaatgg cagaaatcta cttttaataa ccatttacat ttaatgtaat
75061  tattggtatt tttgagccat ttttgttttt catatttata ctacttttga ttgtttttct
75121  ttttcctgat ctatggattc cttgaaaatg ttttttgatt ccattttagt tgatctgtaa
75181  attttttgcg tgactctctg tacagcattg ttagtggttg ctcYgtgtta cattaYgcat
75241  acataScttt tcacagccta gtgctgtgca cattttaccc tattcagtaa aatatggaaa
75301  ccttgctttc ctgtcttttt aactgctccc cctttcccac tgtttgtaac agaatcatat
75361  taaatatatg tctatgtgca tttagtgcca cagtaaattt tgtcattttt gctttgacat
75421  aaatttattg tcttccaggt ttgcagctca gaaatctgaa ctgggtctca ctgcgttaaa
75481  tccatgtctc aaggctgagt tccttcctgg agtctctaga agaaaagctc attatcttgc
75541  cacattcctt ggccctcttc catctttaaa gctagcaatg gccagttgag ccatttgaaa
75601  ctcatgcaga aaacgtgaga agaaagatat tttgtgttta cctactttgt atttccaatt
75661  tttgtttttg gcttgttgtc attgttgttg ttgttgctca ttcttgttgt tgttgctcat
75721  tcttgatgtt ccagaaaaat cctattctat tttttccttt atgattagtg accttcatag
75781  aaccatttta taagataggt ctgttggaaa gMaattctct tagatttttt tttctgtctt
75841  ctaagaatgc ctgtattttc tctttattga tgagtagtta tattggacat aaacatctgg
75901  ttgacatttt ttttctttca gcactttgaa tattttagct acatcctatt ggttattctg
75961  ctctctggta ataacaccac tgttactcac attgtgtttt ccctatagct atggtgtcat
76021  ttgtttcttg ctgctttcaa aacatttttt ttagttttta gaaatttgac tacagtgttg
76081  cttgcatgga ttttcttggg tatattctgg ttggcagttg ctcatcttct tgcatctgta
76141  ggtttatgcc cattgcaagt ttggagaatt ttcagccatt atgtctttga gttctctctc
76201  aatagcatcc tcattgttct tcccttctga agctcagatt ttatcattat cacattattt
76261  gttataacct ttcttgttca taacagtatt taaaatttta ccctgtgttc gagtatacag
76321  tttctattaa tccagcctca agaatactga ctctttcctc catcatttcc tttctgttag
76381  tgatcacacc aaataatatt ttatttaatt ttttacacta catatctcag tatcaaaatt
76441  tctatgtcat tatattatat agtttctttt ttctgctggg aatttttttt ccatttaaat
76501  tgagagtgta cacattgacc tcataatgga tgtttgtaac acctgcttca gtgagattga
76561  aaatctcacc gttttaatat ccagattgtt tcaaggttat catctcttga ttatttaact
76621  ccttgagaat tggtcgattg tttctgattt tataaagtat gttgagtaat tttctatttt
76681  atcccacaca caggactttc tctgtttaca gtacacaata agttcaatga aggaagtttt
76741  ggacattgga gggtagaggc cttcttgctt aattgagatc cactcactgg ttcagatagc
76801  aggggatttt gtcccactaa catttttcta cttggagctt ctgcctggat gatcctttat
76861  ggctcttacg agataaaact ataagtatat ttaacatcat agcatacata gtgtgcactg
76921  aaggggtccc aaaaaattcc tattcacaaa gtctttttta gttttctttt gtgcatcatt
76981  tgctcaaatc tcaattgaat ttgaggactt cattttcctt tcttccctac agtaacaaca
77041  cagtttaaca cctgcttaat tagagtctat tacagtaccc gggtaggcct gctgtcttct
77101  gactggggat ttatagaaag tggataatac ggctgaggta aaatcaacga ctacaggcct
77161  ggcgaaatgg ctcatgcctg taatccaact actttcaagg gcagaagtgg gagaattcct
77221  tgacacaagg aatttgagat atgcttgggc aacatagtaa gacccagtct ttataaaaaa
77281  attacaaaag gatcctggct cggtggtttg cacctgtaga ctcagctatt tgggagggtg
77341  agctgggagt atagattgag cctgggagag caaggctact gtgagccatg attacgccac
77401  tgcactccag cctaagagag agagcaagtc aaagtctgaa aaaacaacaa tgaaaaccac
77461  ccaaaaaaca aaaaagacta gggccaccaa aaaggctttc tttttcactt gacttcttgc
77521  tccagggttt acctttttaa agtgcacctc ccagtgtcta gggagtctac agacttggag
77581  tctcttcctt tcttgcctcg gtatcacaag tggctccatc ctatatcctt cagcactcct
77641  tccccatttc ccacagagcc aagtagaata gtgatttgaa cacatataag caattttaat
77701  ccctaccctc cttgatgctt tacaaaattt tgggatggat ctgttaagct tttgtctcat
77761  ttgagtcagg gatatctcag gcccagctca aatccttaaa ggaactgaga tcagagtaga
77821  ggaatagaag tgataaggga gatatgtata aagtgtcttc atgcaagtaa acaagacaca
77881  tttaatttca aattcaagca gtgcagacat tgctcaaaac caattgaata aacatttgaa
77941  ttttaggtct caccaaaggt ctacaacagt gatggagtat ctttaattct tctacctctt
78001  gttctctatg gaaaaaagca aatgtaaagg tgtcatttgg taagtttgtt tcaattctac
78061  catcagatac tcagttttca accatttccc tatcctactc attaatgaac agcataaggg
78121  aaattttcat acattttggt caacatacca gctattggtg tgtccttttt acattaactg
78181  aagaataata aatagtcttt acagatgtta cgagattaca aaacaaaaag catccaaagg
78241  atctaaatta gggtgtaatg tggatgccta atggttttct atcagaactc tcaccaattg
78301  ccctgtctga tgagagaaat gagcaggagc tctgtcgtgg tgaaggactc tgctgaagct
78361  cccccaggca tttctctact agagttctgg ctttctcaaa atattctcag aataagcata
78421  tattgtcctt ctctggccct tcagaaagtc aataagcaaa atgccttgag cttcccaata
78481  aactcttaca attacctttg cactttcctc atccactctt ctttgactgg accacttcca
78541  cctcttggta gccattgctt tgattgtgct ttgtttcatg attgtactga taaagccatg
78601  ttatatctgc tgtcacaatt ctttgatgaa atgcttcagg atctttatcc ccttatttaa
78661  attttcaggg aagcttcttc tcttgtctgt agctgctctg gttgccatag ttttggcatc
78721  cacagagtga aaaatttgct caacattaat ttttcagtta gaatcatgta agatgaacca
78781  attaaaaaac atgaagggtt ggctattgtt tgtactctta atcttcattc ctcttctatc
78841  atggtatcaa caagatacat ttgatatgaa tactctgtca ccgtagactt gaggttcagc
78901  accatttcat cccttcttga aataacatat ctacttgtga actgctgatt tcttcagcca
78961  ttatccaYat gaacttttca gaaagcatca gtggtttYac cttctttcat gcaagcttaa
79021  ccataaattt tctctttgtt ttcactttaa ttttagaaaa atgtattttg ctcttatagg
79081  ggatgttttg aaactcctgt cttatttttc attattcctt gaaatagctc ctattcagac
79141  atcttacagc atatcagttt aggttcattt tgatgccaaa aaatgtggaa tccttttatg
79201  gtatttctta atataacata cattttcatg aactttttaa agacctacta cattacagga
79261  aggcctttca ataatataag aattgctaca ggtaacattg aattttctag ttgattaggc
79321  agattaaMtt tctgtttttc tgctgacata aatagtgatg tgggttgaga aggaatataa
79381  taccagtggt caaatattct gcagctatgt ggaaagtcaa acagttgcaa ctaagtgtca
79441  catgctttaa gggagtcata gagtagctgc ttgggcctag caggtaccct gatgttaaca
79501  ggatgattgt ctctggtttc accaaacatt acttcaaaag agttactgga catgagcagt
79561  tttgcctagg tagcttacag tagaccttca agtaggtaag gaaggtcaga gagaatgagc
79621  agaggtgaat gccagtcaga tgtgcctctt tgagtgggta gctgaggaag ttccagtcct
79681  ggtgggaggc aggcttctcc ttgtctcctg agtccaggaa gagaacaggt gatgcttcca
79741  tatatcactc aactcacacc ctattcctga ccctctctaa tgtcagaaat gagtgagaga
79801  gatttgtgtt tcaggcaaga ataaatattg ggtatcaacc taccagacaa ccaaaacaga
79861  gattaccaaa gagctgaagg agacactgtg actgacttag aatggagtca aagccaggag
79921  caggactgca gcaggcgctt aggcatctgt acaggagtca aggacctcag caatccacct
79981  gtcatttaaa atgaatgtaa acataccgtg taccaagtag aatcttgatt tccaccttct
80041  ataacctgtt ctctttaata tcaacaatca ttaatttcag accaagaaac cagaatatat
80101  tgagaaacac atcaatgaaa caatacattt cccagaacta aaggttaatt ctccatatcg
80161  aaagggccca atgattatcc aaaagcccaa atttaacatt ttcagggctc agtgcaagaa
80221  gacaattagt ggctaacata ctccaactct taataatcac acattataaa gcaagctaga
80281  ctctaggttt agatcacatt tccatgtaag tccttcagcc tgttgcttgg agattgctca
80341  tataagctgg catagagact aaaggtgtct gtgtttccct gtaacaatcc tgaccccgca
80401  atctgagagg tgggaggagg ggggcacagg gctgtctgct agagaagcac actagacgtt
80461  gggctggagc ccaggactga tctgcccaag cttcgagagc ccctgccagg catgttatgc
80521  tcccctatgc ctcacttctg agtttggact agtggcaatg tctacacagc tcccaatgat
80581  aaccctactg agcataggac tggcagagaa taataaccag aatatacaaa caaacaactc
80641  aaaaacaaca aaatagtcca ttaaaaagtg aacaagagat agaaatggac atcttttcaa
80701  ataagacata caaataatca acagtatgtg aaaaatactc tatatcacta atcatcagag
80761  aaataaaaat caaaaccaca ctatcacctt accctattca gaatggctat gattaaaaca
80821  aacaaacaaa caaaacagat gttgggatgg atgtggagaa aggagagctc ttatacaccc
80881  tgattggaaa gtaaatagta taaactctat ggaaaacagt aYgatttctc aaagaactaa
80941  aactcgatta ctattcaatc tagcaatctt actactgtat ctatgcagag gaaatgaaat
81001  cagtatatga aaaataaatc tgcacttcta tgtttattga aacaccatac acaaYagcaa
81061  agatatggca tcaacctaag taaccatcaa tggatactaa tgggtttgta ggtaaagaaa
81121  tcgtgatata tacacaatgc aatactactc tgcaaaaaaa aaaaaaaaaa aaaaaaagaa
81181  ggaacgaaat cactcctttt gtagcaatac gaatggaact ggatgtgaca gataatatgc
81241  tatgtgacaa aggccagata cagaaagtca agtataacat gttgtcacta ataagtaggt
81301  gctaaaatgt gtttacatag atatagaggg gagaactata gacactgaag ccttggaaga
81361  gtgagtgggt gggagggaat taagagttag acattaatta gtaaatgcaa tRtttgttat
81421  tcttgttatg gaaaccctaa ggccatgatt taaccattat gcaatgtata catgtaacaa
81481  aattatcttt gaaccacata tatttataaa aataaagttt aaaaataaat tgagggtgac
81541  cgggtgcggt ggctcacgcc tgtaatcaca gcactttggg atgccgaggc ggatggaaca
81601  cgaggtcagg agttcgatac cagcctgacc aacatggtga aaccccgtct taactaaaaa
81661  tacaaaaatt agccaggcat ggtggtgcat gcttgtaatc ccagctactc aggaggctga
81721  ggcaggagaa tcgcttgaac ctgggaggcg gaggttgcag tgagccgata tcttgccact
81781  gcagRccagc ctgggtgaca gaatgagact ccatctcaaa ataaataaat aaataaataa
81841  ataaataaat aaataaatat aattgaaggc aaatttaagc atattttttc aaaaaagaaa
81901  aagagggaga tggagtagga gaaataaaaa agaaggaaga ggaggaggag gaggaagaaa
81961  gagcctgcat ttctaattgt cataattaaa tttccataag cagatactat tttttttaaa
82021  aaaaacctaa ttagaatttt acgggtctga taagaaagaa aatgctaaaa aaaatatgRa
82081  tactcaRtgt gttcctattt tgtttgaaag cctagaaaaa aacaatatca gagactattc
82141  tacttagatt ttttggacaa agaaatctta agatttttaa ctcgggaaca tttaattcct
82201  cacaatttca tgtctgctgt tctttcctaa aatcattatt gtgaagcaga catgactata
82261  tccactagtg tttcatgtat gtgatctgct tagtcttcat cctttagggg agccattttg
82321  attattcaaa aatttagaag aggaaactta ggctctgaga cttaagtagc ttcctatgag
82381  tccagagtta gcaagacata gttcttaaac tgcaatctga gagattccaa attctttgcc
82441  cattcaagtc aggctgcctc tgatgaaatc catttaggtt ttcttaagaa tagtaagact
82501  cataggcagg gacagaactg atcagtgata aaatatggga aaataggtct agtggcttct
82561  ggggaataga ggcatagaac catactcatt tccctagaaa acaaaatcgg ttacttttga
82621  acttgttaaa atttctctca gcttctggga ttttctttca ttggtattgt tttgcttaca
82681  ttcataagct gactagaata tcaagaaatg acctgaaaat aattgtacaa cttaaattcg
82741  agctatagaa gacagagaag gaaagaaaca gtggaactcc ttccccaggt ggatgactta
82801  ataaggataa ggaggagaga agcttgctgg aaacatcaag gagaaggggt cctggatatt
82861  cttacaatgt gaacatgggt ctcagattct agagtcccaa acactcagaa agagacacca
82921  ccagcagaaa ttgcaagctg attgttttat tggtataYtg aagttagagc atgatgccat
82981  gtttcacggc atttgaaaca ctgttatctt cttattcact tcctgaaaaa gacaagcaaa
83041  caaggaagat tacagacaac acttgacatg gcaggaaatg tctaatccct cactgggggt
83101  tactgcagtg atggagatgc aagcccctac tcttgccctc tatcccttct acagccccct
83161  tctccttagt aatattttct ttgttcactc tttaagccat tgcacataat gtgatttaaa
83221  gtgttgctta gatgagaaag agaaacaatt attgttatgt gttcatgctt tgctgctgtt
83281  ggttatgtaa gggctatttt tacaaagatg ggcactgcaa catatcatat aatagtgaga
83341  atgtgaatcc Mgccttaata tccagtgaca gggattggtt aaatagatta cggaacaacc
83401  aaacaataga atagtataca attgtaaaaa caaactaacc aRtaaacact gaagagatct
83461  aaatgaactc aKgagaaagt tttctaaaca ataaattttt ccagtgagta agacacaaat
83521  ctgaatatac agtatggtat aagtgcttgt aataaaagca ttatatatat ttatgtagca
83581  atatatattt caaaaagtag attccaaaga gtaaaaggtg ggggttttga gaaggataga
83641  agggaggaaa gatgagttag gctcttacta tttagctctg tacatttcgg ttcagtgaga
83701  ttagtttttg ttagcttaag catgtatgac tttaaatgta agtatactaa aaatgagata
83761  ccattttaca actgaaagtt caaggaccac ttagcaggaa tactggacct gaaagcctga
83821  tgtgaggcag gactgagcaa agcatgttca ggtgaaaact ttaaatggga gtctgagttt
83881  gaggaaagaa aatagagaga gccaaagggg gaacatggtt ctatgtcctc attgtcaacc
83941  ctacttaaga catgtaattt gaagaYagtg attctcaaaa tcagaattgc cttcaagagc
84001  ttctgtaaaa cacagggtca cagggtcttg atactctata caagaatatc tgaataacaa
84061  tttttagaaa tgtgttccag gacagggcaa tattaacagg ttttcagagg attcattggc
84121  acaatgaagt tggagaactg tagcagttgg agcctttgat ggataataaa ctggaatcgt
84181  acctgtcatt gaatcctaga ttactgagga gactgcccct gtggaggtcc ttgtgggcgg
84241  cccccttggg gaggtggtcc ctggggcttt ccaggaggag gtgggggagg accttgctga
84301  tggcctccct gttggggtgg tccttgtggc cttcctcgag gaggacgggg atggcctccc
84361  tgttggggtg gtccttgtgg ccttccttga ggaggagggg gatggcctcc ctgYtggggt
84421  ggtccttgtg gctttccctg aggaggtggt ggaccttgtt gctgctggcc tccttgttgg
84481  ggtggtcctt gtggctttcc ctgaggaggt ggtggacctt gttgctgctg gcctccttgt
84541  tggggtggtc cctgctgagg gccatcatcc tggttcccat caccagcaga gggttgagat
84601  tgctgtcctc ccaaaggtgg tccctgacgc tcctcatcta ggaactgctc agagtctcct
84661  ccatctgtgt gagttgaaac aagaagagct gagctcatgc tggaaaaccc tcctgtcttc
84721  atacctctct gtcttcacca cacggccggc ccctctctgc ctgacctgcc tctcaactcc
84781  caacctcccc ccttcccaag gcttcctaat tagaactcct cttaatccac ataagggtga
84841  tgaaaaattg aatttcttta ctcatggtcc ccagaatcaa ggttgggaga aaactgtttg
84901  tatctttggg gcattggtat tagccaattc ctgacaagaa aatggtgata agaagacact
84961  ggagaactca tcaatttttc agggaaaaat ggagacagag tttactgaga atttattggg
85021  atttacctga tattacgagg ggaacatctt cctggctgac atctagaaaa gaagtacagg
85081  atgatgggaa aagttacttc ctgaatcatt caaggctcat agtgttctac gaggataaag
85141  gacctctgat cacaccctgt gcatcccctt tgtgatctca tcagccactc tctgatgcta
85201  ctggaagtgg aagaagatgt aagggaaagc agtattgtta ctacactgag cgtcaaccag
85261  gaactcaaca tagaagggcc cctgtttgtc ctcttttgat tccccaagcc ttgatgagga
85321  atgaagatac aataggtctt ctgatcctcg gcatgaaaat tctgcagtcc catctgtttt
85381  ctcatcctcc tctcttccct ccactttcct cctctatagc atttgcctgt aaacccttag
85441  cgcttattta gttaaactgc tctgagtatt tcaatgacat atttggggat tcttctgccc
85501  tctttcatct gtaaatatgt tgtttatgtt tgcaagcctt ctcaacagga gccaccagac
85561  atagccactt ggatataatc ttacgcatgc cattcccagc acattgaaat actacattca
85621  ggagacagaa aaatgacaat gtttcactct ggttattctc taactctatg tagacagaga
85681  caagtgttta cccaattctc tgcctcggca gtagctctga catgtgttta tctccttcat
85741  taatgctcat ccactgtaca gcaaggccac catcatccct gtctactggg atcagtacag
85801  gatcttcaca gctgtacttc cctgaatctg tcttgcattc tccacatcct tcacagcagt
85861  cccatgtctc tatataatgc aaatctcact ctctcacacc cctagtacga attctttatt
85921  ggattctatt tgtgcaacaa ataaactatg aggccttgat gaggaatgga ggtacaatag
85981  atcttctgat cctcagcatg agaactctgc agtcccatct gttttctcat cctcctctct
86041  tccctccact ttcctcctct atagcatttg cctgtaaacc ccaatcagag tcacaatatc
86101  ttcccccaat tcatcttacc ttcatttaaa tcctgagctg agctgaaggc cagcagggcc
86161  actgacagca gaatcagaag catcttgcag gaggctctgg tgtcactccc aactttgtgc
86221  tgggagaaac gtgtcagctc cctttataaa gacaagcagg acaatggtgc atttgagctc
86281  cctaccaggt gggcctcctc gcctcagaga ctggcttctg ctttgcttac ttcaggtcaa
86341  gtgtatccct catttctttt gggactctag ctcagcagga agggttgggt aggatattgt
86401  ttgtgtctaa ttcctaaaag gcacaactat gacttggaca aatgttttga cggaactgtg
86461  tccaagcagt cggcacagtg tcaggattga actttagaca tcttttggtt ttcaatctgt
86521  ttggaaagac tgctattctg cttttcactg tgcttttcat ttStgtgtat gtgagtttgt
86581  gtgggtgtgt cggggggtgg acactgatcc tacagctaac agtgaagatg gtcaatatct
86641  ctgactgttt tgatagcctc tttccatctc tcatgatgtg tgtgcgtgga tattttcaca
86701  tttagctaaa tttttcatgt aacagagatg gtcttgctta tctgtgagat gtgtgaggac
86761  agcacactcc caggcacaca tatatgaRag aaaggKgctg gcagcctgct caggctacaa
86821  gaattgtcca aacttctgtg aatctcacta cactcaggca agccttggtg ctatacaaca
86881  caggcaagct aaaatatttg tattttaaaa atatctttag ggtttattaa agcaaaactg
86941  aactcagaaa ccaccaagga atttctagac ctgaacaaac tgaatatatg gttccctaag
87001  tgagccacac gatgtcatat agctaagacc tccactttct ccagaatatt ccatagaatt
87061  catcctctac tttatttctt ctaataaata ttgtaattgt catcactgat aattaaatgg
87121  ccacaattat acattcatct aagataattt gtaatacctg tccaagcctc aattgccttg
87181  attggtcttc tgcttttact cagtaattat gtcacctgtt tcatcagagt gttctttctc
87241  accaaggatt tttatctgta atattccaat actcattctt tctcattctt tttctcgcac
87301  ttatttgttg aaattttgta ttctgagtct accttgccat gtttactttt cgtcctatat
87361  cttttttttt ctccctacaa aggtgtcctt tatttatcct tctaagtaaa ggtgtgtggt
87421  actcctcact acctttactt tcatttttgt ctgtataatt tgacaataaa aaattaagtt
87481  tccaaaaagc cctgtcaggg tcctggtttg gtagctctct cctcagctgc tgattgctcc
87541  aaaaattaag tttccaaaaa gccctgtcag agtcctggtt ttgtcgctct ctcctcagct
87601  gctgattgct ctgtgatacc tagaaattcc ctgYtcatca attcataaaa tatactactc
87661  agaatcacca gctgcacttt ccatttcaga ccttgagtag gtcttgtgta atttttcact
87721  ttttgtgcat ggttagtctt tatttgagga tagcttggtt ggaggcatgg ctgagaagcc
87781  aggtgacaga aaatcataaa gacaaggccc tagacaggtg tgcatgagtg gatcctgtgc
87841  tgggcatatg agggtctggt atggctcttc tgaaaagttg cagataataa tacaagaagg
87901  cttcctagta gattagattt tgctacagac gacattgttc ttgtaaggga cccaggcaga
87961  ttggtatttt ccctggaata aatatagacc tatttcaggg gggaatgcat tcttaattgg
88021  caattcttct ctggccctgt gggaagtaga tcagctctag ctgggccgta tgagttgtat
88081  ctcaggcaca gagtagttgc ttgagcccag cagctcccaa tgcgttatgg agattattgt
88141  ctctggtttc accaggtact gtttcccata ggacctggag atgagcagtc ctgcctcggt
88201  gacttcaggt caaccatcat gtcaaaaagg aaggtcagag aaaaggaaca aagaggagtg
88261  ccaggcagat attgcctgat gatgtggggg actgaggaag ctgcaggcct ggtgcaagac
88321  aggtttctcc ttggcagcag agtacaggaa aagaacagga aatcctccta aaggtcactc
88381  agctcaggcc tgctccctaa cctgctccaa tgtcagcaat cagcgagaga ctagtgtatc
88441  aggaaagcat aaatatcagc tatgctctca ctagacacat caaaaacaga ggtaatggaa
88501  gagctgaaag ggacactgtc tgtgagtgaR ggtgggtgaa agggagacgc aggactgctg
88561  caggagcttg ggtacctaga ggtgggtggc agccctcagc accacacttg ccttctacta
88621  tagatgttca tattctgagg ccctgaaggg ccagtcttca acatatgaaa ttttaggatt
88681  catctctcat aacacatccc aacattttct cggtcactga caggtgttta cctagtggtt
88741  caaaaatcct cgctccctcc aggaacttat gcattagccg atctaattgc ctccactttc
88801  aaaatacgtc ccaaatatga ccacttgcct gatctttact agaaggtgat tgttcagcca
88861  tgatgctctc tcctggagga ctttaccagc ctccagctgg tcttggtaat gttcttcccc
88921  cagctcatcc catatcagtt ccctcacaac aatcagagtg atcatttaaa agtagaaatt
88981  aggttatatc acatttgtgc ttcaaatctc ctgttttttc ttctgcactt gtgataagat
89041  ccaaatttca tgcctcagct ctttcacacc cacatgcctc actctttttt gaaaaccagg
89101  aagctctctg aatggaacag aaagtagaat cttcttcgac ttcaccatgc tctagtttgc
89161  ctggaacact aaagatggtt tgccaggaaa gatgaacatt gRcagaggga ataaacttct
89221  ctgagagtca gtgagtttca ttgggtcgct gtggtctggc agtgttatga attggcagaa
89281  ttattctatt aattgcacca actaagcatt ctgataatac agttagcaaa taaacacaat
89341  agtctctgag acttcaactt gctttgactt gtctaaaaag tttggtgaca ctattatggc
89401  caattaacta ttaaaaatat cttctatatt tttaaatttg atgttagtgg tccataaata
89461  tatatatttc acaattaatt tagcatattt cttttttttt tttttgagat ggtgtcttgc
89521  tctgtcaccc aggttggggt gcagtggcat gatctcagct cactgcaact tccacctccc
89581  gggttcaagc aattctcctg cctcagcctc ccaagtagct gggattacag gYccctcgcc
89641  accatgactg actaattttt tcccatctct actaaaaata caaaaaaaaa attataYcta
89701  aagaaattaa gtcacatggc taRggtcact gtagtaaatg aggaaaaatt aagttgattt
89761  tctcttctct ttgtaattcc agtttaaccg gatgttgtcg atgttgtttt caatttagaa
89821  aatatagcaa attgttcatt agccatgagt atcactctgc tatccaccac tttctaagaa
89881  ggcgcttatt ttagaacaag gctaacacta aattacagga aattaagtaa ttgtggcaaa
89941  ctagagaagc atagtatgta taataggtct tactcagtta gatccacctg tcaaggctga
90001  gtccctttct ggagtctcta gagaagcacc cattttttgt ccacattcct tggccttctt
90061  ccatcttcaa tgccagcaat agccacttga gcctcccatc acatagcatc acgtcgccct
90121  tgcactgact cttctgcctc cctcttctgc agttaatgac cttatggtta cattgagtcc
90181  acctagataa tccaggataa cctttctaaa ttcaggtcaa ttgattcgca aatgtaattt
90241  ccctttgtca tgtaagttaa tatattcact tgtctgagaa ttaggaagtg tgtgactttg
90301  ggagactgtt aatcttcata gaacacacag gatggacacc ttacccatct agacctggtt
90361  tggctcagtg agagttggag agactcaccc aggcttcttc cagatatttg catgagaata
90421  tgaatgagtt tatgtataat tagttgttat acaaatattt gtgacttaaa gtatatatct
90481  atgcaaaagc gtaaatcaca aactgagatg gcaaaaattt actttcaata accattgaca
90541  cttaatgcat tttttgatat tttagagctg tcatattttt tgttttttat ttatactact
90601  tttgattgtt ttctttttcc tgatctccta tggggccact gaaagtgttt tataattcta
90661  ttttagttga tccgtataat ttttgagtgt atctctctga acagtgtttt tagtgattct
90721  tctatgtttt actttacatg tacatagctt ataatctagt ggtgtacact ttttaccagt
90781  tttagtgaag tatggaaacc ttacatccct ttataatttt taggtcaaaa tgaatttatt
90841  atttttcagt tttgtaggtc agaaatctga actgaggttc actgagttaa atccatgtgt
90901  caaggctgag tttcctccta gaggctctag agaagaatgt catttttcca catttcctgt
90961  ctctcttcca tcttcaatgc tagcaatggc tggttaaaac atatgaagaa aactcaagtg
91021  aaaaaggaag atatattgtg tttgcccact ttcccccttt ctSttatttt tacttcattc
91081  ttgatgttcc acaaatttct cttctattct ttcctttatg tttagagaac ttcatttggc
91141  tattatttaa agataggttt gttggaaaga aattctccta ggtttctagc ttttcttttt
91201  ttcttagaat gcctggattt cctttacatt aatgaaggat atttttattg aacataaaca
91261  tctggtagac catttctact tttgagcact ttaaatattt gtgctccttt ctactgttat
91321  ttctggtttc tggtSataat aaccactatt atttatattg cattttccct ataactatga
91381  tgtcatttcc ttctttctgc tttcaatatt ttgtctttaa ttttcagaaa ttggacaatg
91441  aattttcctg gaatggattt cattggttgt attctggttg ggatttgctc agcttcttga
91501  atcYgtaggt ttatgctctt tgctgtgttt ggaaaatttt tgatcattat ttctctgagt
91561  cctttttcaa catcaacctc ttctgctttt ctgaaactca gaatgcatga atagtacaYc
91621  atttgttata gcctttcata ttcatgatat gatgtataat tttttcaatt attttaccct
91681  gtattgttta gagtatataa tttctattga tccaacctca agaacactgt ctttcctcca
91741  tcattttctt tatgctattt atcatgccca ataaaggttt tttaagacat tatatttttc
91801  atttctaaaa tatctgtttt cttaatttat aatttataat tcctattttt ctgctgacaa
91861  gtttttcctt tccattcatt ttgagtgtct accttgacct catgaaggat gggtacaaca
91921  gctcctgtaa agtctttggt cattttaata tctaagtaat ttcaaggttg taatttcttg
91981  attgtttcat tccttgagaa atggtcgatt ttttgtgagt tgttttgagt acattgagta
92041  attttctgtt ttatcttgca cagagggatt ttctcttttt gcaatacaca aaaagcccaa
92101  taagggtagg ttcggacatt ggagggtgaa ggcactcatg gtaaattatg ctccaccaac
92161  tggctcaaat ggccagggag tttgtcccac taacattttt ggtggttttc cctgggtcaa
92221  tctctctggc acttatgaga taatatttta agtatttaat atcacaccat acatacagat
92281  ataaagacaa taaaaagcag tgcatcgaat gggtccaaag ggccctatcc acaaagtctt
92341  cctaactttg gtgtgtgtgt gtgtgtgttt gtgtgtgtgt gtgtgcactg tttgttcaat
92401  cctcatcagt tgaactcagg cacttctttt tcctttttct ttctaaacct gcttgttaga
92461  ggctgtcaca gtagccagaa ggccctgcta tcttctattt gagtatttat agaatgcagg
92521  aaaaaagagg gtaaactata aagaacgact gtaggcctcc ccggtgcagt ggctcatgcc
92581  tgtaatatca gtacttggag aggcagaggt gggaggactg cttaaggcca ggagttctag
92641  accagcctgg gtgacatagt gagactcctc tgtctttaca aaacactaaa aaattatcca
92701  ggcatgatgg tgtgtgcctg ttgtcctagt catttgggtg gctgaggtgg gaggaaaaga
92761  agggaaggaa agaggagggg atgggagggg aggggagggt tggctttttt tttttcctct
92821  tggcttcttg cccttctttc tagtgcacct cccagtaggg tctcttttgt atttcctcac
92881  atgctattgt cctccatccc tcagcacccc ttcccccaac ccccagaacc aagtagaatg
92941  gtgatttgtg cacatatatc caacagggct atactaattt gagtccctac tctccttgaa
93001  gctttgcaat atgttgggat ggttctgtag ggtctttggt gtcccttggg tcaggaagat
93061  ctcaggccca gcccgaatcc ctaaagcaat tgacatcaga atagaggtaa agaagttatc
93121  agggaaatat agagaaagtg tcttcaggcc agtatgcaag acacatttac tttcaaataa
93181  gcattattgc cactactcgg tggttaactg aataagcatc tgaatgtaac aactttggga
93241  gatccttaat cttttcacct ttccaccaaa ggcctgtaac aactttggga gatctttaat
93301  ctgtctacct aatgttatct ggagaagaca gctaaatcta aaagtgtcat ttggaagtct
93361  gtttcagttc taccagtggt actcaatttt caaccacttc ccaatcctcc tcattaatga
93421  gcaacataat agaagaatat ttatttacca attacaaaca ttttagtcaa catgttagct
93481  accagttccc atggactttc agcaaatcct attaatcagc ttgtaggatc cttcactttc
93541  tcctcagaag gccaggtctt tgggctgtgt tccatcctcg ttgccaaaat tattataagc
93601  taacaactta gcttgtcact tgttgtactt gcagaaggca aaagatggtt tattattcac
93661  tgaagtacta gcagtagtca gaggaatatc atcgtactat ttcttcaagc ttcaRtcccc
93721  tcacagggat aagatgagaa actgatgtta catgtgcaag tagtggtgtc tatcaaaaga
93781  gaaatctcaa aattaggaaa ctccagtctt aaaaactttg atcactgtga gtaacctgtc
93841  catcttgcct tctgaagaag aagctttaca ctggaatgta agcaaatatc tctggagaga
93901  agaagaagaa gagaagagtc tttatcttta aacaaatctg ccttYtgacc aagagggata
93961  ccctatttct aaatcccaag gctctttgcc atgacctggt cttgggcaaa gtgaacgaga
94021  ctcctagggc acaaaacgta aaaaggcatt caataggttg cagggcccta aaaccaagtg
94081  gctcctcaaa ctttgcaccc tgcatgcctt gctcacctga tcctagttga ggcccttctg
94141  tttgctcatc aaacatactg tttgctcaca agataagagc tagaccatgc agaagtgcaa
94201  tacattcatg gagaattttt ttcccaacat ccatYcccaa gatcaaggtt acacagttgc
94261  caaagattgt agagtattct gaatatatca tctattttct tcctggacag aactaaatcc
94321  atcagaagta acttttttaa aaaatgtttc tttcttttgc atacaatttg tttttatttt
94381  agattaagag atgagtccag aggtaagggg gacaggagaa ctgtgacatc agggatgaca
94441  ctaccctttg agagcactgg agcagttaga aaggattgaa agtgaataat cttatttcat
94501  aaggggtatg tcattttatg attcaaatta agtctgtgat tatcacttag caaaattgct
94561  aaaatcatta tcagaatatc ccatatcatg ataggaggaa gaaaaagaag taaaatctaa
94621  aattgttatg gaggtaaata gaagactgcc ataaacaagc tatctacaga attatggggc
94681  ttaggaattc acagtaccct ataaacaaga acacagcttt gagaatttct atactctaat
94741  ttttaaaaat gaagtatatt ttgtgaattt ttcacccaca aattatttta actggaagcc
94801  atcacagctc attaatcagc aattggtgaa ggactaaagc agcccaaatc attcctgtct
94861  caaagtaagg cctgcttgta agcaagtgtg aactggggat gtatgtgcag aaatcagagc
94921  ctaagtatgg taagctggac atttcagaga taaggaaggc agctggtgtg tgtgaaatct
94981  tatatcaata actgatttta ttttttcagt cctaattgct attatggaat ttgtgctttt
95041  cattattaca tcttcacaaa gcatgatttt taactttaga acaatttaca ttttatgctg
95101  gataattctg ttgtgaggga ctgtcatgtg cattgtgaga tattgagcag catccttgtc
95161  ctcgtacagt tatcaggtaa aatacagagt gtgtcagttc aatttgaatt tcaaataagc
95221  aacaaataat tgtcattgtc tgtgtatcca attatcaatg ctgcaggtct gtaattggtc
95281  ctccatagca cacttacatt atgatgtaaa ctcatattat ggttatcaca agtaatactt
95341  atttataaat ttattcttgt tgttttttct tttgtctgaa atgtaaattt aactaggcct
95401  cctatatttt tatttgctta atctgttaac attacaatag atgccagcag catccgtcca
95461  attttaactg aaatgtctcc aggcattatt aaatgtcccc taggggggca aaattgccct
95521  gtgtggagaa ccactgtcct aaagggattt catcctttgc acatggaaat ctcgtggtct
95581  attctctgga tattttaatt ttattcacac atttaaaatt tcgttttagg cctgcaatgt
95641  ccaggttatt tttctagggt ttactttttg ttctatcgat taccatataa ttaacttttt
95701  aaaatacttt ctgcaatgtt gaaaatttgt cttttgtctt tactgctgaa aattatatta
95761  tttgcttgca atatgttctt ttttttctta gagagaacgt aattgttctt caaaatctgc
95821  ttccaattat ttcattttaa atatttatta Ytgttacctt aaacttgcga ttgtgttctt
95881  ttatttaacc tgctattttt acatgctttc tcattacatt tttaagagga gacgttctta
95941  ttaaattcaa ttctgtaaac tgattggtta tccctgattt gtttttcttt tcaaaacttt
96001  atttattttt ctttctttac acagtgactt tcatcaaatg agatcctgta tttgttacta
96061  tcgtcttggt cttctatatt tttatttact aaacctggta actctacact agatgccagc
96121  agcagtcctc caattttgct tacttaaaat cttactgatt ttttaatacg tgtccttgcc
96181  ttccttacac agttaaccaa ttgagtaaaa ttttattgtt agtccaaaga ttcagattct
96241  acagtacaag tctgttgtag atggtcaaga ttgtaacatt tttgatggtc aaagttgctt
96301  agtgttttta actgaaaatg actgaaaata ttaacagaat agggcttata tttggaaata
96361  actcttagtg atgtacattt caattgactt tgtttcatat cctcaaagtt ttcactaagc
96421  tgtagactct actaactatt ttcatttgag ttttcctttc tagttttaaa ttatcaattg
96481  atcatcattt ttaggatagg aactaaatcc ctatgtgttt gtacatataa atctgatttt
96541  atatacactc tatacacata catagatatt ataattcttg atgtatttct attctattct
96601  aatgcttatg caacatatta ataaaattta aagatagagt aatctattat attgatcaca
96661  gtgcctaatg aaaaatgcat tttcaatata ttagaatttt actattaaat acataatctc
96721  catatatggt gcaaacaagg tagcaatctg ttctgcttaa tttaaatcag caagtatttt
96781  caataatgct caatatatca tagaacataa tgagaaaata agaggtaggt gaaagaaaca
96841  aatgtgtagc cctcctgttc ttcttccttt ttttctttct gagaatatca tcactcatat
96901  caggcatgtt tataaaaatg agagattatg tcctttttgg catacttcat cttcttcagg
96961  acacagagag aagcttgctt cagtttgctg tcccgtaaaa ttagaagaaa tgaatggcca
97021  gatggatgga aaaataccag cacactacta aaagtccttg ccgcaacgaa gtctagaaga
97081  ggacaggcca atgttagtat gatactgatg aaataatgca caataaagag gagtagaaat
97141  gaaattatag cttttatagg ttttacatgg gcttctgtgc tgggatccct ggaataaata
97201  ccatgtagct tcatctgcct ggtgtggctc cataaggaga ggattaagag aagaatggaa
97261  gccagtgttt ccacaaaagg aaggatgaac atcatctgaa ggagaaacag agaagtgaaa
97321  tcatgcatgg tatccatata attaaatgtc aagttgcctt ttattgttac ccgttcttag
97381  attaaactat taattattat ttccttcaga atggaagttg tgcagaaaga gatcgttgcc
97441  tctagtacaa taaagagaag caccttgtga attttccatt tcatccagag gaaaatggga
97501  ttggagaagt tggctacctt gaggaaatag aagacactga ggcaagtggt acaggacagg
97561  cagaaatagc tggatcctgt ccagagaatg tcaaaactta ctgcaagatt cttagaatca
97621  ggcattttct catagcctat attgaaagag gtagctaaaa ttgttatcta caggaaacat
97681  atcctggaga tggccaagca gttgagaata aaatcaatca gggagacctt tcagttcctg
97741  attcagtcaa tgatattaac caatacaatg aatcaatttc ccacagtccc cattatgaat
97801  tacactacaa gaattcttat aaacagtgtc tcccaaatgc ttgacatgcc tctacatagt
97861  gaatctctgc cttcaataca ccattggctg attgatccta gctgcactgt tatagattta
97921  tatatccaaa ggagtgactt ctttctgttt tattgatggc cattggtgta gtctggagag
97981  atccaaggtg tgttctaaaa ttagatttca actaaattgt aaaatataca agtttttttc
98041  ccttgagggt atcagctagt gattttcaag gcaaatatta tattccctgc tactattgac
98101  tctggttgta ttttacatgg aaagttctgc attcaaattt gaaccctcat ttgttaacat
98161  gcaaaattga agtagattct cctttatagt tttacaatgc catcttttgc ttaaaggtta
98221  ttcatttgta tgatgtatcc ctttgattca tctaccaccc tgggctagaa attctaagcc
98281  cccatataga aaaatgcagt atatatttag agtttatgat cagcatattt cttcattatc
98341  aacctcacta atgcagatta tttataaaat atgcaaagtt taagaaaatg gagaaaactg
98401  ctctctgata taataccaaa tcttttagag agtatggaag tttttggcca ggcacggtgg
98461  ctcacgcctg taatcccagc actttggaag gccaaggcag gtggatcacc tgaggtcagg
98521  agttcgagac cagcctggcc aacatggtga aaccctgtct ctactaaaaa tacaaaaatt
98581  agctgggctt ggtggcatgc tcctgtaatc ccagttactc gggaggctga ggcaggagaa
98641  tcgcttgaac ccaggaggca gatgttgcag tgagccgaga tcacactact gcactccagc
98701  ctgggtgaca gagtgagatc cgtctcaaaa aaaaaaaaaa aagagagaga gagtatgcaa
98761  gttttaaaac atacgataca tactttggca cttcttctat tgatatctta ggagtctgtg
98821  tactctgtcc ttaaatctag aatggtttgt gactaattca actaataaaa atgctataat
98881  aattctaaag ctaaatcatg aaaagccatg cagttttttc ttaatcactg caatatttac
98941  tcttggagtc ctaagccaca tgtaagtagt ccaactactc tgaggtcaca atgtccaaac
99001  ccacatgcag aggccatgtg caggccctct ggctcattat cccacgtgag cccagctttt
99061  acgtcctttt accccaggta ctaaacctat gagccaagaa agcgatattt gacctgtctg
99121  atacggtaga tactagccac atgtcacacg tggcttttta aattcacatt aataaaaatt
99181  aaataagatt aaaagtacag agcctcagta ttactaacca tatttcaaat gctcactagc
99241  cacgtgtgac tattttatag ccaacataca taggggatat ttctatcatt gcctatcatt
99301  gacatttcta tgtatttctc agctccgcct tagataagtt cagcccctgg tcacttgaat
99361  tgcccccagt tgttcataac tttctggcta aaggcttaga cattctggaa caaaaagaag
99421  ccatccctgc tatgtcttga acaaaaccct aacctacaga atctttaagt gaattagtct
99481  attcttgcat tgctataaag aaacacctga aactgggttt ctgtaagagg tttaattggc
99541  tcatggcttc acaggctgta caggaagcat ggcggcatct gtttctgggg aggcctcagg
99601  aaatgtataa agatggcgga aggcaatggg gaaatcagat acatcttcac atggttggag
99661  caggaggaag agaaagatgg gggaggtgcc ttacactttc aaacaaccaa atcttgtgat
99721  aactcactca ctcactattg ccacaccacc accaaggggg aaactgcccc ccataatcca
99781  atcacctccc accaggcccc acctccaaca ttaggggtta caattcaata tgagatttgg
99841  gcgaggacag aaattcaaac catatcaaca agcacaataa aatggctgtt attttacacc
99901  atgaagtttc tgggtgattt tattatgcag caatagagca tcagaataga attatttagc
99961  atatgaatgg tatcaatatt ttgtcatttc taatttccat gtctgttttt ctcttctatc
100021 ccaaataaag gtatccatag catctttatt ttcctcagtg taaggggaac ttgtataaat
100081 tttctataat tttttcatac gttgagctga acttaatctt aattctgatg aggtcaaacc
100141 aggaaaggcc aattagtcat gttctttgtg taatgcaact gctgcatttc cctcccaaag
100201 tagctagtcc aaaatttagt gacattcaaa accaatggag atataatata ggcctaagag
100261 gagtcaaaga aatgaatgag ttggacagcc caccaaaaga tacaccctag gtaaattcac
100321 aaagaaaact ccatgactaa aagaaaggct aattgtgata ggcaaacatg aaatgcaaac
100381 aaaactgaaa ctgaaataaa caaagggaaa ttcaggaaaa tattatatgc tgaaaaaggg
100441 ttattttatt tgtcttattg aatatcttct ttgttccagc cctctaggag gtgggatgaa
100501 gcattaaata caactatttt taaatgccct taataagttg acattctgaa aagatgaact
100561 ctaaacatat aactaataaa atatatatca tacagatata aaatatagtt tataagaagt
100621 tgcaaatata agtagagagg gtcaatagaa agcatgtcag aagaggggtt gaaattttaa
100681 ataaggtgct tagggaagga ctcattgaga aggtgatgtc tgaacaaaga cctaaagtag
100741 gtaaagaaat tatccatgtt ttgtctgggt aaagtacatt ccagacacac ggaacagcat
100801 tatcagtgtg ctaacattgg atgtacctag tgtgtctgag gaagaacaag gaaagcagtg
100861 tgactgttgc ggagatgaga gagtgagacc actaagagtg aagtctcaga aagaagaggc
100921 atgtgcccag aaggcagcac atacagtcaa aagagattat tttagaacgt taatatttaa
100981 cgcctgccct gctgtgtttt gtacttgcgt ggtgcctact gctcctctct tgtggctgat
101041 ttctcccttt tggaatgaga atgtctatcc aatgcctgta caacaactgt atcttggaag
101101 gaaataaatt gttttttatt tcacaggctc atagctggaa ggaacttgac ttccagtctc
101161 aaatgagact ttggacttca gacatttgaa ttgatgcagc aacgtgttaa gaattggggg
101221 actattgttg ggatggaaag attacatttt tttttatgtg agaaggacac gagttttcag
101281 gggaccaagt cagaatgcta tggtttgtac acggtttgtt tgtccccatc agaactcata
101341 ttgggatttg atccccaaag tagtggtgtt gggaggttga gcttagtgag aagtgtttga
101401 gtcatagggt tggttccctc atgaatgcct tggtaccatt ttcaatgtag tgagtgagtt
101461 ctcactctca caagactgaa tttgttctag gggaatgagt tgtttttcat gagagtgaat
101521 tgttataaag ccaggatgcc cctttggttt attctctctt cacatgtgcc cgcttctgct
101581 ttgaccttct ccaccatggt ttgacccagc acaaaagctc tcagcagaaa ctaagcagat
101641 gctaatgcca tgcttcttgt acagcctgcg gaaccatgaa ctaaataaac ctcttttatt
101701 tataaattac tcagcttcag gtattccttt ataccaacac aaacagacca agaaattgag
101761 gaataatatt tataaaatta tttcagtgaa ttaaattaga taaaatggac aaattccctg
101821 aaagaaatta ctgaatcagg cacacaaaga aatagaaaat cttagtgtct atttttaaaa
101881 attgaattct taatttaaaa ctttcacaca aagcaaggga tgacctgaat gtttattttc
101941 atcacatatt taagaagaaa aaaataccaa cctacataaa tttttcaaca aagtctcata
102001 aaggttaact ctgtgagctt tatttcaaga aggaaagtgg gaatctacca gaatcataag
102061 gacacacaga tcccagggag gggaacatgg gcaaacagcc cccatgatgg cacccagtta
102121 aaaaagtgaa tgaagctcca gtaggtgaga gaggcagaga gcctccctct gtgactcacc
102181 tttccactgg ggatctgagc aacccagtgc tcagagggaa gagtaccttg tttctccaaa
102241 gccctggaga taacttggag aaaggcttag agacattgta agggaaagac actgggaaaa
102301 ggttcaggcc ttttctcaga cccaggactg agagcaggat accattttta attcagatgc
102361 cacaaagtca atcattcttt ggtaactaag cagcgtagct gtgcaggcat tttagtcttg
102421 ggccagatat tggagcacct gctttagagc agggtgggga cctccatggc cagaattgtg
102481 gggaaaaaaa accttagcag caaacactga aactgtgcta tcccccatca caggcctggg
102541 gcaggagggg agctgctaca gccagtttat cctatgtgat gagacttgca accaggcagc
102601 ttagctactt ggaactagtc tgtgtgtgtc attgctgggt accccaccct gttcccctgt
102661 gattgtggtg cagcagactc tcctccactc tacctctagg cagaaatctg ggcatttgga
102721 tcactcattc acctggttca gcagcttcat ctgctccacc cttcttgtgc agagatccta
102781 gaatagggag gccctctctg cttcatgcct aggcagatcc caggattttg gagcacctgc
102841 tagcaaggtt cagcaccctg aaccacctaa ccttccttga cgtaggtcat ggtgcaatgg
102901 ggccctctct gctccacagc caggcagaac tctagatatc tgaagcaccc attcacctgg
102961 ctcagcagcc tatcctcccc tacccttcct gtgcagagat tttgatgcag ggaggtcctc
103021 tctgctgcat accctagcat tcaaagcacc tgctcacaca gactggcaac ctgtgctgcc
103081 tcacccttcc cgttcacaga tccatgtact gggctgatcc tctctgtttt atgcccaggc
103141 aaatatccaa gcattaagag cacctgcctg cctagttaag aagcctgagt caccccattt
103201 accctgtgca gaaatcttgg ttggtggggg tgggttctgc tccacaagca ggcagatttc
103261 caggtattca gagcccccac tctcctggat taagagttta ggccaatctc tccaatatcc
103321 ctgtgcaaag aacttggagc cgagggggtt acctagctcc acccctagac agaactctgg
103381 tcacttggta gccactcagt agattctccc tcagtgttgg tgcttgtgtc tgccattgga
103441 agacctatag gtggacctgc cttgtctggc cccacccacc ttgctcccca caacccccaa
103501 ggctgagcag ggagttcaca ccactgtgta ctccatgagt cagaccatta tctgcagcaa
103561 cagagagttt cttccagtaa acaaggatca aatatataac catcacattg tccaaaactg
103621 gctcttacct ataagtgcca tctactggct tgtaggttga accgcacagt ctaatataaa
103681 acctgcaacc tgtaagtgca taaggctata gaagctgggc aaaagaccta cccagaattc
103741 tctaaagtca tacctcctaa ggagggaggg ggaatggaaa gggaaagaaa aaagaacaat
103801 aatattatag ggaaataaag aaaaaaatcc taccttatgg aaataattac aaaaattaga
103861 agtgccagta ttgccagagg aggaggaaag agaacaagaa ttctaccacc ataaaaaaag
103921 tgaatgtaat gaaatcaaca ccatcaaagg agcacagtac ctctccagca atggtcccta
103981 accaaaacaa aagctcagat atgacagata aaaaattcaa agtatagatt gcaaggaagc
104041 tcaatgagat gcaagacaag gctgaaaatg aatacagaga gatttctaaa gcaatccagg
104101 aaatgaagga agacataaac atcttacaaa gaaatcaatc agagcttctg gaattgagaa
104161 acttacttaa ggaattataa agtacaattg aattcatata aagtacaatc tctcatagac
104221 tggaccaagc agaagagttt cagaccttga aaactggtct tttgaactaa cccagaaaaa
104281 aaaattaaaa ataatttaaa aatatgaata cagtcttaga gcaatatggg attatgtgaa
104341 gcaaccaaac tcataaactg ctggcattcc tgagaaagaa ggagaaatag ttaataacct
104401 ggaaaacata tttgaggaaa taatttaaga aaatttccct aatcttgcta gagaggtaga
104461 catacagata caagaaatca agaggacacc tgactgatgc tataggaaat gaacatcacc
104521 aaagcatata gtcgccagac tatccaaggt ctacactaaa gaaaaaaatc ttaaaggcag
104581 ctagagaaaa agatcagatc acatacaaag ggaaccccat aaggccaagt atatagctca
104641 gcagaaacct tacaagccag gagagattgg aggcctactt tcagcattct taaaggaaag
104701 aaaattttcc aaccaataat tgtatattcc atcaaactaa cctttataac tgaaggagaa
104761 ataaaatctt ttatagacaa gcaaagggta aaggaattta ttaccactgg accagtctta
104821 caagagatcc ttaagggagt tctaaacatg aaaatctgct accaaaatag cacacctaag
104881 cacatagccc aagaccctat aaagaaacca cacaatagaa actacaaagc acccagctaa
104941 caacttcaaa atagaatcaa aacctcacat agcaatatta gccttgaatg taaatggtct
105001 aaataccctc acttaaaagt cacagagtga caaattcaat aaaaaacaaa agtcatcggt
105061 ctgctgtctt caagagaccc atctcacatg taatgttact gttaggctca aagtaaaggg
105121 ttgtagaagg atctgtaaca caaatagaaa agaaaaaaag aaagaatcac tattcctata
105181 taagataaaa cagattttaa accaacaaca gtaaaaaaat gacacagaaa ggcactacat
105241 aataataaag tgttcaattc aacaagaaga cttaactgcc ccaaatatac atgcactcag
105301 cattggagca ccaagattca tgaaacaagt acctccaaac ctataaaaag atttagatac
105361 ccactcaata atattggggg acttcaacac cccactgaga agattggaca gatcattgag
105421 gcagaaaact aacaaggaaa ttctggactc ttgaaaaaac gctcaaataa ttaggcattg
105481 aaggaacata cctcaaaata ataagagcca tctatgacaa aactacagct aacattacac
105541 tgaacatgca aaaactgaaa gcattcccct tgagaactgg aacaagaaaa gggtccccac
105601 tcccatcact cctattcaac atagtattga aagtcctagc caggacaatc aggcaagaaa
105661 aaaaaaggca tccaaataga aaaataaaag ccatagtcaa actatatctc tttgctgatg
105721 tgaatccata catacaaaac cctaaagact ctgccaaaag gctcctggca cagataaagg
105781 acttctgtaa agtttcagga tacaaatcaa ttcacaaaaa tcagtagcat ttctatacaa
105841 taaaaatctt caagctgaga gccaaatcaa gaatgcaatc ccatttacaa tagcctcaaa
105901 aaaaaaaaaa aaaaaaaaaa caactaggac tacagctaac caaggaggtg aaagagaact
105961 gtgaaacact gctgaaagaa atcatagatg acacaaacaa atggaaaagt attccatgct
106021 catggatagg aagaatgaat attgttaaaa tggttgtacg gcccaaagca atttacagat
106081 tcaactctat tcctatcaag ctaccaatgt cattttgcac agaactagaa aaaaaaaact
106141 attttaaaat tcatataaaa ccaaaaaaag agcccaaata gcaaaagcaa ccctaagcaa
106201 aaagaaaaaa gccagaggaa tcacattacc tgacttcaaa ctatactata aagctacagt
106261 aaccaaaaca gcatggtaat attataaaaa cagacacata gaccaatgga acagaataga
106321 gaacccagaa ataaagacac acaactacag ccatctgatg tttgacaaaa ctgacaaaaa
106381 taaacaatgg ggaaaggact ctgtattcag taaatggtgc tgggatatct ggctagccat
106441 aagcaaaata attaaactga acctttacct ttcaccatat acaaaaatta actcaggatg
106501 ggtcaaaaat ttaaatgtaa gactttatac tacaataatc ctggaagaaa acttaggaaa
106561 cacaattcta aacattggcc ttgggaaaga atttatgact aagtccccaa aagcaattgc
106621 aacaaaaaac aaaaattgac aagtgggacc taattaaact aaagaatttc tgcacacatt
106681 gtataaacta tcaacagagt aaacagacaa cctatagaat gtaagatttg caaactatgc
106741 atgcaacaag ggtctaatat cccaaatcta taaggaatgg aaacaattga acaagcagaa
106801 aacaaataac ccctttaaaa acgagcaaaa gacatgaaca ggcacttttc aaaagaagac
106861 atacgagcag ccaacaaata ttttaaaagg ctcaacatca ctaatcatta gagacacgca
106921 aatcaaaacc acaaagagat accatctcac accagtcaga atagctacta tcaaaaagtt
106981 aaaaaaaaaa aaacagatgc tagtgaagct gtggagcaat gggaacgctt gtaccctgtt
107041 ggtgggcatg caaattacgt cagccactgt agaaagcagt ttggagattt ctcaaagaac
107101 ttaaaataaa ctaccattca acctagtcat cccattactc agtatggtgt atgcacgcca
107161 cacttttttg gtctaatcca tttttttttt tttgagacag agtttcactc tgttgtcctc
107221 tcactctgtt cacaagaaaa taaatcattc taccagaaag acacatgtac tctcacaatt
107281 tcattggaac actattcaca atagcaaagg catgggaatc aacctacgtg cccatcaaca
107341 atggattaca ggccaggcac agtggctcat gcctgtaatc ccagcacttt gggaggtcaa
107401 ggtgggcaga tacctgtggt cagcagttca agaccagcct ggccaacatg gtgaaactct
107461 gtctctacta aaaatacaaa aattagccgg gcgtcctggc acctgcctgt aatcccagct
107521 acttgggagg ctgaggcaga agaatcgctt gaacctggga ggtggaggca gcagtgagct
107581 gagatcatgt cactgcactt cagcctgaga aacagagtaa gattctgtct caaagaaaaa
107641 aaaatggatt agaccaaaaa agtgtggtat atatagacca tagaatacta tgtagccatt
107701 aaaaaaaaat gaaatcatgt cctttgcagc agcacggatg cagctaaagg ccattgtcct
107761 gagtgaatta atgcagaaac agaaaaccaa atactgcatg tttcacttat aaataggaac
107821 tatacaattt taactaacac tactaagtga tagaattgag aattgtacct ctgactccag
107881 actcagtcct taactattat agtgtttttt gtggggacca tatgagtgca gaggtgaaaa
107941 tactgacaga tattgaaaga tgctattggg taaataaact acaagagagt tcagagaaat
108001 taatgataaa aatgatcata ctactcctct cacttaccta acttttaagc aaacccattt
108061 gggaggtcct ttctaaaaag aaaataaaag agagaaaatg agaatgctca agtagatagt
108121 actattgaaa caggttacta aaaatgcatg tggtatcacc aaagaacctg ttctgacagc
108181 agagaaaatg aaattaggct ttctgtcctt atactcaagg ataactgtgc cttattgcaa
108241 atatttattc caactgttcc ataaaatatc tttgagtttt gaaagcaaaa aaagttaaaa
108301 ataattttct tacaggaaaa tagttgatta ttgaacttca ttaaaagttt ttaatgtcct
108361 ctctttgaaa gacactgtaa agaaaatgaa atgacaagcc tctggctggc agaaaatatt
108421 tataaaacat atgtctgata aagaacttat attcagaatg atatggtctg gctgtgtccc
108481 cacccaaatc tcatcttgaa ttgtagctcc cataattccc atgtgttatg ggagggactc
108541 agtgggagat aattgaatga tgggggtggt ttcccccata ctgttctcat ggtagtgaat
108601 aagtctcatg aaacttgatg tttttataag gggtttcccc tttcgcttgg ctgtcattct
108661 ctcttcactg ccaccatgta agacgtgact tttgtctctg ccattattgt gaggcctttc
108721 cagccatgtg gaactgtcag tcaattaaac ctctttttct ttataagtta cccagtcttg
108781 agtatgtctt tatcagcagc atgaaaatgg actaatacag agactatata aagaacattt
108841 acaactcagt aataaaaaca gaaagcaatg aatcaagcta agtagttggc aaaaggttta
108901 aacaaacatt tcataaaaag aggttgtatg aatgacccaa agcatatgaa aaaatcatca
108961 gcctcactag ccattaggaa aatgtgaatt aaaactatga tgagatgcca ttacattctg
109021 actagaatgg ccaaaataga aaacaaaaca gagtacctac caactgttgg ccagaatgtg
109081 aaataactga aacactcgta tattgctggc aatagtatga aatggtgcaa ccactctata
109141 gaactctggc agttttttct taagttaaac atacacttat catatgattt gacattttta
109201 ctcataggta tttactcaag agtgaggaaa ttgtatatcc agaagaagag aaatacatga
109261 atgttgatat cagttttatt tataatggcc aataattgaa aaccatttga atgttcatta
109321 acaggaaaga gaaatttttt aatgtggtat atccaacact actcaggaac actgctcagg
109381 aacaaactac tgattaatac aacaaaacag aagaacttca aaaacattat gctgagttaa
109441 agaagcctca catataacaa tacacactct ataactgtat gtgtgtgtgt gtgtgtgtat
109501 atatggcatc tatatatcta tatctataga tatatagatg cataaaattc tagaaatcaa
109561 aaactctctt attgttacca aaagcagata agtagttgct tgggaaaaag ggctggaggg
109621 gagagggtaa actgcaaagg gcactgacat tgtggtcaca caagtgtata cattgtcaaa
109681 actctaactg aaatgtggac taacatgttg cattttatta tatgtaaatt atacctcaat
109741 aatatttact ttaaaaggca taatgtaaaa tatgtatgta cacatacata caatataatt
109801 actaaaaaac cgttatcttt ttatgtggca gttactcata ttaaactttt taaccctctc
109861 aattctctca acacatcagt atagtaatag tggcaagtcc aaacttccct aattctattc
109921 caaataactg ttctaattat ctgtagctca ccatttaaat aggagtactt gagataataa
109981 aataattacc taaatcaccc atctcaatga atgatttaga attgaagagc cattgccaaa
110041 caaaagattg tagatttaca attaacatct aagtatgctg tagtatatgt ttttaattta
110101 caatagaatc tgccaataat ttcctcaaat aagtttcaaa ttcctaataa tgtagaaata
110161 catgtcataa atacataata ttcttaggta agatgctatt atagagaaga atttaaaaca
110221 tttacagtga ccttcacaat aaaagaaacc aggggaagca taaaatatct tttaaactcc
110281 ttgtagactc ctgtttctgt ctgcaatatt caataatctg tggtctgaat ggcttatgaa
110341 attgtgagtt tctcatcgtt tagcccatac cttagctgcc accaaaagaa aggcctgtct
110401 taacttagcg tttcctagaa tcagaagaaa ggagtggctt gaaggagaga agactccaat
110461 cgtctcacaa agcatgtaga tcactgtgtt ctgatacagc tcagaaatcc atgatatgag
110521 aacacataga aagaaactag catagaataa aaggaatgag atcacaattt tcaaggcatt
110581 tgtatggacc ttggtcctgg ggtctctgtg tcctttgtaa ttgagttgca ttttctggag
110641 atgtttctgc agggagaaaa ttaacaggag aaaagagatg aaggccacag taaatggtgt
110701 tagactgaac atagtcatag tgaatttgac cgacactgaa aatgtttcaa agtcactcat
110761 actgaaattc caagttgtgt ttctttcata tcggtccagc cagtctttta tatgcatgtt
110821 tatttgtatc agatttaaaa ataagaagac caaggttcct agcagtatca tcagaatcac
110881 tttgtttact ctccacttca aatagagaaa agcagggcta gagaaactcg ctattttgag
110941 caaataaaag atgctgaaga ttgtagcaag ccagagattg aagtgattag aaactatcca
111001 gctaaaaatc ataattctta atcctgttcc agacacaaat atggctagat aatgcagagc
111061 taaaaaccaa cttactaata tttcccagat cagcccaatt ctggagattg ccaagataat
111121 gaggagttta tcgactgagg acagctctct tttactgacc cagtcaatgc agttgatcag
111181 tactataaat ccattgctca aattcccaat tatgaattct gcaattatta caagagtgaa
111241 gatactcggc agggcacttt ccatgtcaga acagagaaag ttcaatgtct aatgtcactg
111301 ctggttattc actgatctaa aatgctattc acatccttga gtgtccagtg gagttcttct
111361 tccttctcct ttttctgctc cttctttcat tgttggctca acgtcaaagc agaaatctct
111421 aaagtttgct gatcgatctt cacataactg ttctggtgat atctttattt ttcttcaatt
111481 tctctgctga gccctagcta agatatttat gtcttcacca tgggcagaaa tatttcatag
111541 atgattatgc agcaaagtta aactcacatt tgcaaccatg caaataaaga tatattctct
111601 ttcattgttt tgtacttttt tgccttgtct gagcatagaa aattagattc aaccagcttg
111661 agttctgagg tacaaatatt ataaaaatct gattcataga atatgtagct aaatgaagct
111721 tttatggcta atagcatagc caatgaagct ttataaaata tgcaaagact tacctatgct
111781 atttcaaaag agtgctcaat ttcttgtgga gctaaagctg gatctggtca atactgtgac
111841 taaagagaaa ctcgtttaca aagcatccat ctttctcatt ccctgcctca tcactactta
111901 gcagtgctca ccaccatccc tccataggca ccaaatgcct tcacatttta tccgcttttc
111961 ccctcatatt tcttttttaa acttaaaagt gataaaaaaa caaattttac aaaatcagca
112021 acagctacca aagaaagcag tactatatta catcataact tgcaaaactg aaaaccaagc
112081 aaagaaatag atacactagt tccccttatc catgaggcct atgtcccaag acccccagtg
112141 gatgcctaaa acagcagata gtaccaaact ctatatacaa tatatttttt tcctgtattc
112201 acctacctat gataaagttt aatttgtaaa tcaagcacag taaaagatta caaaaataac
112261 taataataaa atagaacaat taaaacaata tactataata aaagttgcca ggggttgtgg
112321 ctcatccctg taatcctagc aatttgggag attgaggcgg gaggaccgct cgagcccaaa
112381 agtttgagac cggcctgggc aacataggga gaccctgtat ctaaaataaa taaaaattaa
112441 aaaaataaaa agtaaaaaaa tgtgttaaaa tatatagtgt ggtgtctctc actctctcaa
112501 gataccttac tgtactgtac acacctattt ttggactgtg gttgaccttg ggtcactgaa
112561 actgcagata agcggggact aatgtgttca ttgtgaagag tcaaggttcc tcaccccgcc
112621 ccactctttc aaaaactacc gaaaaaaagt ggattaccaa aattctgaaa acgtggagac
112681 tcaagaaaga ttgtctaatt caaaattaat ataaataaaa cactttatct tgattataag
112741 ataataagta gccatttagc tagatgaata caaagctatt tttatttaat aaagatataa
112801 tttgtgaatt atgaatgttt gtattttatt actcatgcaa tctttattgg gtgatctaca
112861 gagtactaaa aaatttacaa aataattgca tcatctttaa tgatttgcaa gtttcaatta
112921 taacttaaaa taggtagata ttttctattt tgttgttata atggtatatt tgtcaagata
112981 gaatagaaaa tgtctccttt aacagtttgt taggtttgta tatttatgac ctttaaatat
113041 gtagctatgt ggcatgtggt cttccatttg cattcttgac ccagggctga agagatcttt
113101 tatacttttt cattttaggg tgttcttcct acctatctag agaaaagggt ttggggatgc
113161 cggtcacaac ttgataatac cccaaatctg tacaaatagg cctgaggaat ggtactgtac
113221 ttgctggtat atgaaaaaat accacagttt ttagaacttg tgatataaca catgacaaag
113281 acatagagaa tggataacag gcttgcggtt ctgagctgca ttttcctggt gtgtttccat
113341 aagagaaaac atttaaaaat ataaatttaa tcaaagagat gtagtctctc acttctataa
113401 gagaaagaga atagatgcta gggagcatca cagaaaaact ttttcagcag tctgaactga
113461 aaaggaaaat gaggcaaagg gcaggaagag gaaacaatga aaggtttgag tatgtaaagt
113521 attttttaag tcactgatgg atgcagtttg ccaacgttgt gttaggatat ttgcagctat
113581 gtttttaagg aatattgaca tttaattttc atttctcata ttgtgcctat gagaatttgg
113641 tattaagatt tagctaacct catatcattt gtttagaaat atttcttttc tctttttcat
113701 ttttgctgcc tattattgtt acatataaca aatatagaac atttaatgaa gtttaaatgc
113761 tatagtataa agtgcacact aatttaaaag cctcccatgg caagaaagag aactttgtaa
113821 accccataac accttatttg ctcctttcta atcacaaccc ctatcttcat tccagaagta
113881 acaactatct gcaattcaaa tcacgttatt gtttaacttt gtatatttta atttgaatat
113941 gtatccctaa gcactagctt ggttttgcct ggttttggat tgcacatgaa tggaataata
114001 ctgtacacaa tatttttgaa tttaactatt tttgatcaat attatgtttc taaaattaat
114061 catgttgcat atagagatag atcatttatt caattgctgt atagtattaa tggaatgaca
114121 attcaaaatt tatctagtct agtggaaaca caaagttggt ttattttgag tttggagact
114181 atgatgatga tgatgctacg aacattcttt aacatgtaac ttattattga gtacatgagc
114241 atacatttct atgatcttga tctggaacca ttccgttgcc tatttcttct ggtcttgttt
114301 tggcagttac gactttctag atatttgtca gtttcaatta tttatgtttt catgtaattt
114361 tgattataat atcatcttat taaattttta ataccaacag aatatatcct ttaaattctt
114421 catattattt gcttttcttt tcatgattta tcttaccaga agtttgccta ttttgttagt
114481 ctattcaagt ctgtcttggt cgatcttttc agatgtatta tttttcatta atttcatctc
114541 ttatattgtt atttaattat ttttacttcc gttgagtatg tttgctgtta tattcctggc
114601 atacgttttc ctcttagaat ttttcagcat ttctcacaag gttttactat attttattat
114661 acttaatttt aaatattttc taaatttatt gtgatttttc ttccaccata gactattttt
114721 aaaatatatg ttcgagtttc catttacata aaattttctc tgctaaaatt tatgttatgc
114781 atatatggtc aattttgata aatgttttgt gtttgctgaa aataatgtgt atcctgtagc
114841 tttggggtgc agtgtttcat acatccacta gatctatttg taatcatgtt atttaaatcc
114901 tatttacctt ttctgagttt tgtcttttta ttctattatt tcttgagatt taatgttaaa
114961 ctatcctgct atgagtatag gtttatctat gtcttttaga agtcctgtca acttttactt
115021 tacatatttt gatgccatgc tgatgagtgc atagagcgtt agaattgtag tatcttcatg
115081 gcaaatataa cctctactca catgaggtga cattatctat tctaatgtct ttttccttaa
115141 aatctattta gcctcacgcc tgaaatccca gcactttggg aggccgaggc gggtggatca
115201 tgaggtcagg agatcgagac cagcatggct aacacatggt gaaaccaagt ctctactaaa
115261 actacaaaaa attagccggg catggtggca ggcacctgta gtcccagcta ctcgggaggc
115321 agaggcaaga gaatggcagg aacctgggag gtggagcttg cagtgagcca agatcacacc
115381 actgcactcc agtgtgggtg acaaagcgag actccgtctc aaaaaaaaaa aatctattta
115441 gtatatatta aaatgattac attagcttat ttttgttagt gttttatggt gtgatttttc
115501 taaattttac ttttaacttc tctttgttat taggatttag atatatatct tgtacatagc
115561 atagaggtcg taaaatggga cctagagcta tatgctaaca ttttattcct tgtaggataa
115621 gaaatgtagt cttgtaggag tcccctctcc cccttgaagt tgtatattat gtaaattttt
115681 ggtcaataaa atattgtgga aatgacatgt tgcttcagag tgagagcatt tactcaccac
115741 tataagactg caaatttctc atcttcttgc cttgacactc aaacaagggt caataaacca
115801 tctatcagcc tggattcttt tgtgaccact atgagcacag gacatgtaac agaaatcaaa
115861 aacaaaaaca aacaacaaaa acttctccta cgtggagtcc ctgagctttg gggatcttgt
115921 tagtacagca tagactaagc tatcttggca agtatgagac tggcctcttg aactggtatg
115981 ttactaaaac aaaacaccta acttatgtga cactggctta atacttgatt cattggcagt
116041 gagtggcaag aaaaaacatt actgtttact gtaaaacaag atagggagtg gcaaaacatt
116101 cgatagcagt cacctgtggt tatgtgggag gtaaatcaca tatttgaaag ttttgtaact
116161 ctagaaaaaa aatataattg aatatattaa tggttcattt gacaattaaa aaaattgact
116221 ttagaaaata attgcctact gtctagctaa atagcaaaca gagggagatg ctctaaaaga
116281 aaatgatatt tatttgggag taatattgca atgagaatac atgtgccata gtaaattatg
116341 tgtgtattca gggacattag aaaagacaag gatttctaaa agaggaagat tacataattg
116401 ttttgagata attaaccttg gatccaagga tcaggaacaa gggtgacacc tgtctaaggt
116461 tagacaagga ggttctgagc agatgtcctt gcagaagtat ctttttgtgt agggttgcag
116521 tggcctttgt gcaatgttgt tgttttgtag agtcttttct ggtagctctt gttataagtc
116581 gtatttacat taagaactct cccctcatgg ccttctccag ctccatttgt cagagtttta
116641 acacaagtga cttcattttg attctgacaa ttttatacta ctttgcaagc aggaaaaaaa
116701 tatatcagaa agaagagtct agaaacttga gattttgcag agctggaaga ggcaactgct
116761 tctcaacact gaatagtatt tgagtttcta agcctggtta aaactcagcc atggctgggc
116821 acggtggctc acgcctgtaa tcccagcact ttgggaggct aaggcaggtg catcacaagg
116881 tcaggagatc gagaccaccc tggctaacac ggtgaaaccc catctctact attaatacaa
116941 acaattagcc tggcatggtg gcgggcacct gtagtcccag ctacttggga ggctgaggca
117001 ggagaatggt gtgaacccag gaggcagagc ttgcagtgag ccaagatcat gccactgcac
117061 tccagcctgg gccacagagt gagactccgt ctcaaaaaaa caaaaaacaa acaaacaaaa
117121 aaactcagcc ttaagactag gattatctca agggtatggt tgttacatcc atgttaaaaa
117181 ttctgactag attatattta cagaaaaagg ttcaactgac agttactgct ctttcagttt
117241 gacaaggtaa tatcaaggca gagtgattaa atatctggtt tcctgaatga agccttacaa
117301 tgctcaaagt atctgatatt aaatccagag agaaaagcag aggggtgaaa aagcaacaaa
117361 acacagcaaa tataagaagc agcagactag acaaaaactg tgacttgttt gcttgtatgt
117421 gaagctgaca ggaattaagt agactaaaaa attgagtttc ttaaaaaatt gtgcttctga
117481 aagaaatgcc aacccaaact aggtcacacg tgtcaaagta tattaaaatg gaggccaggt
117541 ctgaagaatc cctaggcaga caaagccagt taggtctcat atgaccttaa aactgattga
117601 tttacaaaag taagcaaaaa ttaacttgag ctatttttta taaatgccta tagtaaagaa
117661 aaacagaact taaactcaac caatcaaaga cagccaacag gatagccaaa catttgattg
117721 gttagctata aagttacctt atttcatctg tcctgtcttt gcttttcttc tttgtctgtc
117781 ctataaaagc atccccatcg cgttccctcg gtagagctcc ggaaccacct cagtttggag
117841 ctttccaatt tatgaaccat catttgcaaa taaactttta aaaaacttta gtgtgcctta
117901 gttgacctta ttaacacatg ttgagctaaa accctgtatt agtctgttct catgttgcta
117961 ataaagacat acacaagact aagtaattta taaaggaaag aggtttaatg aactcacagt
118021 tctacatggc tgaggaggcc tcacaatcat ggtggaagac aaagaaagag gaaagggaca
118081 tcttacgtgg cagcagacaa gagagagttt gcaaggcaac tcccctttat aaaaccatca
118141 gatctcatga gaattactta ttcattatca taagaacaac atgggaaaga cccgcccacg
118201 attcagttgc ctcccactga gttcctcccc atgacacatg ggaattactc gggagccaca
118261 attcaagatg agatttggat ggggacacag aaaaaccata tcaaaccctg ttccagagat
118321 gcagtttaac atattatgag acaaatggga gctcacagac aatatgagaa tctcttgaag
118381 attacacttt ttttcctagt tctaaaatta catctttttt aatgagactt atattcttcc
118441 cccaactcag aacatttcct tgtctttaag ggagacctgc cacagataga gaaccataga
118501 aaataacagt aacaaccaca taaactgtca tgttagctat gaaaagagtt ggtggaagga
118561 atgggctctc accaagacag tgaaaaatag aggtaggtgg taaacaaata tgcaaaagga
118621 ccattcagaa acccaagcaa actatcattc tccccatttt actggtgcta taaatacctg
118681 aaaaaaagtg ccacagaaat ggattagtgt tatcatcaac aaatatattt gagccgggca
118741 cagtggctca cacctctaat cccagcattt tgggaggcca aaatgggaag atatcttgag
118801 cccaggagtt tgagaccagc ctgaacaaca taaaaagacc acctctctat aaaataagat
118861 aaaataaaat aaaataaaat aaaataaaaa aaatagctgg gcatggtggt gcacatctgt
118921 agtcccagct acttagaagg ctgagatgga aggattgctt gagcccggaa ggcccaggct
118981 gcagtgagcc atgcttatgc cactgccagt cagcctgggt gacagtgaga ctcatatata
119041 tgtatacatt taaatatatg tatacatggg tgagactcat atatacagat atgtatatat
119101 ttgtacgtat gagataagct ggtgagaatg aggtcattga agcagatatc ctgttccttg
119161 ccaattaagt cagtgatgaa ttccatagtt aataccacca tgaaaatgtc ctcctctata
119221 cctggcttct cttcagagag aaaaattcct aattttataa ttttttttat tttcaactaa
119281 gggattaata caataattaa gggattaaat atttaatatt tattgtttca gtacttctct
119341 cttgctatgt ccattaattc cattgctcat tgttaatttg ttgttaatta cattaaaatc
119401 agttcttctg taggatcatg tcatattgat ctttatgaaa tcagagcaat catttttcaa
119461 atgattcagc tggcaatttt catactaaca aatattttag ttttttataa gagccacagg
119521 tgataacttt ccatattcaa aagaataccc agtgaagtct agtgttctca gatgcagtta
119581 cggacagttt tcctttcact gttttcactt gttgcttcac cctaaacttc aatttatgta
119641 cacattacag tccccaatta ggagactaac aggatgttga agaatggcat tggcaggaca
119701 tttctgaatt cagtagcagg aatgaactca aaaccagcag tactgagtca gttcttgttc
119761 tcttctgact gaattcgtgt tcatggaaat gtttctgcaa gactgagtgg tcacagtgtc
119821 agaaggctcc tcaggttttg cctcttcaca catgtccatt tcccctttga ccttctgcca
119881 tgtcaagata cagctggaaa gccctcacca gaagctgagc agatatcagt gcctgctttt
119941 ggaacttccc agcctgcaga aacattaggt ctttatagat tgtccagctt caatattatg
120001 cagccttaaa aaggaacaag atcatgtcct ttgcagggac atggatgaag ctggaagcca
120061 ttatcctcag caaactaatg caggaacaga aaaccaaaca ccacgtgttc tcgcttataa
120121 ttgggaggtg aacaatgaga tcacatggac acagggaggg gaacaacact tagcgggcat
120181 gttggggcag gacgggggtg ggagagcatt agagaaaaga gttgatgcat gctgggctta
120241 atatctaggt gataggttga taggtgcagc aaaccaccat ggcacacatt aacctatgta
120301 acaaacctgc atatcctgca catgtacccc agaacttaat aaataaataa ataatacatt
120361 tttaaaagat ccacatgatc aaaaatgcaa aaaaaaataa attatccaac ttcaggtatt
120421 cttttatagc aacataaaat ggactaagac aaaactctgt ggtgaaatct ccaattattt
120481 gtaagatgaa gtcgtagaaa tgggtgaagt ccaaaaagca ggttataatc tatataaaca
120541 tttaagactt ttattaatat tgacaaagtc ccctacaaaa tgcatgcacc aatgtaagct
120601 ctcactagca ttaaacgaga ctgctcgtaa ctttgcacac attataattc tatgtaacaa
120661 tttacaaaag caaaatgtgg ccaatttgaa atgcaaaata gagcacttgt tattcatttt
120721 accatagtac tatactaaac ttacgttttt gtttaaatgt gaggctgaat tttttatata
120781 tgataatctg gtttttaaaa ttatttttat cttttttata aaacagtaaa agtattaaat
120841 ttttttctac tgtttatatt gcaaatagac tttaaagttt ttaatctatt atttaccttt
120901 ctatatttat tttcttgagg cattcagaaa ttcttccagt catgctatta aatgcattca
120961 ttttaaatgt actctgcctt taatatcatg tttaaacatg tcttcccaac tttcaagatt
121021 acaaaacaat tcttggtatt ttctctgttt gattgtttac tttttcaatt ctatatttaa
121081 tcctcttatt tactttggaa gaagaagtat gttacacatt tgtcttattt caaattgcaa
121141 tattgttgtt acaatactaa ttattgcagg gttaattttt cttcacatat ttaagatgga
121201 aaaatctatc tcacacaata tatttgtaaa caaaaaaaga tatttataaa ctctataatt
121261 tttttgttgc actggagttt cctcattctt tcatgatgtt attctatgcc actctttaaa
121321 gagaagtgtt ggaggacatg aaaggtgaga ggatagacat gtaactattt gttggacacc
121381 taacttagga tggacagatt atattatttt tatcagcaaa tgtgaactca tcagtctttt
121441 ccttaatgac atgtgcttcc tcaaattaaa ggcagtagag tcttattttg attttcttgt
121501 aaaagtttta aagaattgtt ttcaaatcta aagtgatagt acatctaatg tagtttttct
121561 gtttgttgtg agttagaaat acaattttgt cccaatatta aatgacagag gtctcaatat
121621 cattagttga acagtctacg tttttttttt gttgatttct aatgccaaat ctatcacgta
121681 cggaggttcc tatgtgataa gaataaacaa tctggtctca ccagacctgt ctttagtttg
121741 atctgctgac ctattactta ttatatacat tttatttctg tgtcacaacc ttcattgttt
121801 tgattttttt aataattttt ttgagacacg gtcttgctct gtctcaaaaa atattaaaaa
121861 aattttttta atcttttttt tttttttcct gacagagtct tgctctgtag cccaggctgg
121921 agtggctcaa ttaaggctca cttcagcctt gacctcctgg gctcaaacaa tcctcctgtt
121981 tgattctctg tgtagctaag actacagttg ccactatacc cagctacttt tttgatgctt
122041 ttagagacaa ggtctcactg tgttgctcaa gtgggtcttg aatgccttaa ctcaagtgat
122101 cttcccatct cagcctccca acatgctggg attacaagta tgagccacta aataaataat
122161 ttaaataagt cttgctatct actggatacc cctaccttaa ttttcttttt caagaattgt
122221 ctgaattatt tttgaatact taattcatgt gtaatttagg actgttttgt caatttgaaa
122281 ataaatttct ttaagattct ggttagaatt atattgaata tgtgaattaa tttgagcata
122341 atcaatgtct ttgaaaatta atttttgcat atctatatag ttcaaatcct tcactaaagt
122401 attatagtta tgtaatttca caatatataa catcatgaaa cacttttctg agacttagtc
122461 ataatcaaaa tatagttctt atttgtaata agaaagggaa tttttttaat tccacatttt
122521 aatggaccag tgctggtgaa taggtgtaat tttagcccat atatattgat attgtactca
122581 gcaagcttat caaatttgtg ttatttcaaa agttttcctg ttgattttta aattatctag
122641 gtggaattac tactattagt atataatttc tttaaatttc catccaaatt tctatgtaac
122701 aatatttttg tcaaagtgta ttagttaaat gacctaacga aattgtcaat aaacaagtat
122761 ctttgtctta atcttgatta aatgaaaagc tctatgttac actaataact atgcttctgt
122821 atttgtttag tagacataca agttattaaa tgttgcttct atttaaactt ggtttgggta
122881 tttatcttga ttaggagtta aattagatta aacatttttg caacatctgt tgagataatt
122941 ttatgtaaat taaaattatt tactttctag ttttaaatta tcttcaaatt cttagaatga
123001 atcttttcat ttgtaatata ttttaagtat atattatgag actaatttac ttttacactt
123061 ttttttagca gttttgtgcc tgtatctaat tgcctacctt ctcattattt gctgtcctct
123121 actgttttta ctcactttga gaataatttt ttaacttctt aaaataaatg agaataaaac
123181 atttaggttc actgaagtag tttggagaag gtaaaaatta tgacattttt aaacttagta
123241 aaagtttttt ataaatccat ctgaatttga tatcttgtgt atattttgat agtccattca
123301 aaatctttga aattgttgat ttattaaagt tttttactac taatataaat ttttgttaac
123361 ctatgctttt taaaaacttg gcaatttcaa ctgagttttt aaattttgga ataaaattat
123421 ttagaacttt gattatataa agctctgcta tttagtattt tctacatttc atcaattttt
123481 ctgcttctcg tatttcttga tcagtaccat cagacttttg aatttcctaa tctttaaaag
123541 attttggttg tactaccatt gtctattata ttttgatttt ctatttattt attcattcat
123601 ttatttattt actactttga ttttggttga catccaaaag cataattact gcccttcaga
123661 caaattctaa cttttccttt tgctgagtct atgacttggg aaatttactt aaccttgcta
123721 tcccttggat ttttgtttgt ttagtttttg agacaaggtc tcacgctgtc acccggggct
123781 ggagtgcagt ggtgcgatct tggctcactg tagttttgac ctcgcaggct caagtgatcc
123841 ttccacctca gcctccctag tagctgggac tacaaaggtg caccaccata cccggctaat
123901 ttttgtattg tttgtagagg gcagtggggt ctcaccatat tgcccaggct tgtctcgaac
123961 tcctgaactc aagcatccac ctgtcttgac ctcccaatgt tcggggatta caggcaggtg
124021 ccacagcacc ctgtcatgcc tctgattctt taaaaaccaa agggaatgag actgttttta
124081 ggaataaata taaaataaat agcacaccta aaccacctag aacagtacca tgtactaagt
124141 aagcgcaagg tacatattag ctccttctta acttattcac ccttctttaa atttttctgt
124201 tgttcatttt tcttaactga aggtgaatat gtgcctcatt catctggaaa acttttttat
124261 aaaacatgta ctaatggata tggattctta gctttagccg gttttttcat tttgattgtg
124321 aaaagctttt tcgttgctga gttctaaaca tttatctttt atgtagaaat gttcatcttt
124381 ttctcagtta tagagaatag ttcaattttc aaactttttt gattgattta acattttact
124441 actttacatt ctacgtaata cttattccat ggaatctgtt ctttgttata gtgtacatgg
124501 tttaggtgtg taaatgtttc atgtgtattt gaatgtttgt gtccagattt ctgtcaagta
124561 cagatttcta cataaaagta ttgaattaat ttttgctaat tgcattctta aaatattcca
124621 tatcattctt tcttctgctt gatatggcag tttattaagt gtgtcttaca ttcttattat
124681 ttgtggattc ttcagtttct gtttgtgagg cttctgcttt atctatttac gtaatgcatt
124741 gttaggtatg tccaaattca tgattattgc atcttttcca tgaatttttt ctttttcttt
124801 tttttgagac ggagtctcgc tctgtcgccc aggccggact gcggactgca gtggcgcaat
124861 ctcggctcac tgcaagctcc gcttcccggg ttcacgccat tctcctgcct cagcctcccg
124921 agtagctggg actacaggcg cccgccaccg cgcccggcta attttttgta tttttagtag
124981 aaacggggtt tcaccttgtt agccaggatg gtctcgatct cctgacctca tgatccaccc
125041 gcctcggcct cccaaagtgc tgggattaca ggcgtgagcc accgcgcccg gccctttttt
125101 ttttttttga gacagagttt tgctcttgtt gcccaggctg gagttcaatg gtgtgatctt
125161 ggctcagtgc cacctctgcc tcccaggttc aagtaattct ccttcctcag tctcctgagt
125221 agctgggatt acaggcacac gccaccatgc acagctaatt tttttgtatt tttagtagag
125281 atgggggttt caccacattg gccaggctgg tcttgaactc ctgacctcag gtgatctgcc
125341 cgccttggcc tcccaaagtg ctggaattac aggcgtgagc caacatgccc agccttcgat
125401 gaattgtttt attcttacta ctctgtaatc acctgtcttt aactgttact gttgtttact
125461 ttaaatgtta tgtgattatt agttataatt cagttaaata tgacaaatat aaacaagcaa
125521 ttgaatatat gcatgtggaa gagcatctat ctttcacata actaaaaaat acataggaaa
125581 gtttttgtgg cacaaattgt ccaagggaat agtgtgattc tatcaccaac aaatgatagg
125641 ataatagaca ttaggcataa gtagcagtta ctgtgctcat tagttagtgg tagattctac
125701 ccatttattt tctgtcactg tggttttctg ctttaggtat gcctcttgaa agcagaacat
125761 agtcgtgttt tgttttctta tttattctct gtcttttgat agatcaagca aatttattta
125821 aattcatgaa catttctgat aacattgaac ttgcttttac tgtcttatta aaattttgct
125881 agttatcact ctcttttcac tctttttttg ctttgagatt aaagtgataa gttcttttta
125941 ttcatttctc catctgtttt tttttttttt tttttttttg agacggagtc tcgctctgtc
126001 gcccaggctg gagtgcgatc tcagctcacg gcaagctccg cctcctgggt tcacgccatt
126061 ctcctgcctc agcctcccga gtagctggga ctacaggcgc ctgccaccac gcccagctaa
126121 ttttttgtat ttttaataga gacggggttt caccgtgtta gccaggatgg tctccatctc
126181 ctgacctcat gatccacccg cctcggcctc ccaaagtgct gggattacag gcgtgagaca
126241 cggagcccag cctcatttct ccatctattc taagtttcta tttttatata acaaatcatt
126301 ccccaaattg caggcttaaa acagcagttt attacttttt tcctcacgat tctgtgaatt
126361 gcctggactt agtggatagt ctctcattgt ggtatttcat gtagttgcca tcagataagg
126421 gatgggacca cagtcatctg aaggctcaaa tgggatgaac atctacaatg atccaactca
126481 atggctgaca tagggaggat tccctctttt tctactgatt ggaatagttt cagaaggaat
126541 ggtaccagct cctccttgta actctggtag aattcggctg tgattccgtc tggtcctgga
126601 ctttttttgg ttggtaggct attaattatt gcctcaattt cagagcctgt tagtggtata
126661 ctcagggatt caaattcttc ctggtttagt cttgggaggg tgtatgtgtc gaggaatgta
126721 tccatttctt ctagattttc tagtttattt gcatagaggt gtttatagtt ttctctgatg
126781 gtagtttgta tttctgtgga atcagtggtg atatcccctt tatcattttt tggggggtct
126841 atttgatttt tctctctttt cttctttatt agtcttgcta ctggtctatc aattttgttg
126901 atcttttcaa aaaaaccaac tcctggattc atggatttct tgaagggtgt tttgtgtctc
126961 tatctccttc aattctgctc tgatcttagt tgtttcttgc tgtctgctag cttttgaatg
127021 tgttggagag cagtttctca taaaagcttg tggattcaac tgtaaaagtg gcaatccact
127081 ctgactcccc tctccactgc agagagcttt cttctttcac ttattaaact tttgctccaa
127141 cctcaccctt ttgtgtccac actccttaat tttcttggtc atgagaccat gagctcagat
127201 aacaccttag taataatatc aatgacccgg acctgttttg gtaacaccag ctctggggcc
127261 aggacacagg ggcactctca ggcagaagac tgaaatctgt gcaagtggta tacaaattta
127321 tcccttcata cacaaatctc aggccagtct ctctcttgga cttctaaaat tttacattca
127381 ttgtgatgag aatcaacatt tttggttcat ttcaaaggac aggctcaagt tttgccaatg
127441 ctaatatggc tcagcagaaa aatgcatgtg gcttgtatta agaaagcaca taaaggatct
127501 acattgactg ggttttcata atcacaaaaa acgaataaat ttgtatgagt atctttatat
127561 gaaggcaagg aggaaaacag gtgaatggaa gcctacgtaa atgtgcttat gatttaaaac
127621 aaacttattt cctcaggaaa aatatatttt tcacctcacc tgttaaagtc ttaggaaagc
127681 ttaaccttga taatctccta ataacagaac ttatgtttct tttctgaacc tcattccttc
127741 tcatattgta ataatgaaat gaataaatga atagcaagtg agtagtggtg cacactaaac
127801 tctgagtact gactgccttc tattccactt aataccttta taagaacatt gttatgtacc
127861 taagtcatgt catctataaa actaaggtaa tgataacaat catatgtccc taatataatg
127921 gttttgatga ttaagtggaa catagtaaat gtttaaaaat gttttctatt ataaccaatt
127981 tggctaggat aggtggaaag aatagaaata tgataattaa ttgtgaaatg tataatatat
128041 attaataggt catcaacatc aaattaaaaa atatagatga ttaccatgat agtgaaacca
128101 aagtttttag acaagtcaaa gcaaagttga agtctcagag accattgtgt ttatttgcta
128161 actgtattat cagactgctt agttgataca gtgaatacaa taattcttcc aatgtacata
128221 aactgccaga caagcaactc aatgcaattc actcactctc ggagaagttt atcccctctg
128281 ccaatgttca tctttcctgg caaaccatcc ttcagtattc caggcaaact agagcaaggt
128341 gaagttgaag aagatcctac tttatattcc attcagagag cttccgggtc ttcaaaaaag
128401 atttagtcat gtgggtgtga aagagctgag tcatgaaatt tggaccttat tgtaagtcca
128461 gaaggaaaaa caaggggttt gaagcacaag ggtgatatga tctaatttct acttttaaat
128521 gatcactctg attgtcgtga gggaactgat atgggatgta ctgggggaag aatgttacca
128581 ggaccaggtc gaggctgtta aagacctcca ggagagagca ttgtgggccg aacaacaagc
128641 ttctagtaaa gatcatgcaa gtggacacat ttgagatgta ttttgaaggt ggaagaaatt
128701 agataagcta atgggtagtg cctggaggga gtgaggattt ttgaaccact aggtaaacac
128761 ctgtgagtaa cagagaaaaa gttgggatgt gttatgaaag atgaatccta aaatttcata
128821 tgttgaagac tgatgcttca ggacctcaga atgtgaacat ccatagtaga agggaattgt
128881 gttgttgacg gctgccaccc actctaggtg cccaagctcc tgcagcagtc ctacttctcc
128941 ctttcaccca cactcactcc cagacagtgt ccctttcagc tcttccatca ccccttcttt
129001 tgatttggct ggtgagagca tagctggtat ttatactttc ttgatatgct agtctctttt
129061 gctgattgtt gacattggag aaggtcatgg aagggacctg agctgagcga cctttaggag
129121 catttcctgt tcttttcctg tactctgctg ccaaggagaa atctgtcttt caccaggctt
129181 gcagctttct ccgttaccca catcatcagg caatatctgt ctggcacccc tctttgttcc
129241 ctttctctgg ccttcctttt tgacatgatg gttgacctga aatcaccaag gcaggactgc
129301 tcatctccag gtctaatgga aaacagtatc tggtgaaacc aaagaaaata atcccagtaa
129361 cccactggga gctgcttggc ttaagcacct accctgtgcc taagatacaa cccatcaagc
129421 ccagctaaaa ctgatccagt ttccacaggg ccagagaaga attgccaatt aagaatgcat
129481 tcccacttca aatatgtcta tatttatttc agggaaaata tcagtctgcc tgggtccctt
129541 atgagaacat tgttgtctgt agaaaaatct aatctattag gaagccttct tgttttgtta
129601 tctgcaactc ttcagaaggg ccagaccaga ccctcgtatg cacagtacag gatccaccca
129661 tgcacatctg tctagggcct tgtctttatg attttctgtc acatggcctc tcagccatgc
129721 ctccaaccaa gctatcctca aataaacact agccgtgcac aaaaagtgaa aaattacaca
129781 aggcctacac aaggtctgaa atggaaagtg cagctggtga taccgagtag tatattttat
129841 gaattgagga acagggaatt tctaggtatc acagagcaat cagcagctga gaagaggagt
129901 accaatccag gaccctggaa ggatttttca gaaacttaat agtttattgt ccaattgtag
129961 agacaaaaat gaaaataaag atagtgagga gtaccataca ccattgctta gaagattaaa
130021 taaatgtcat ctcccacaag tcatgtgggg agaaaaaaac tatataggaa gaaaattaca
130081 aacgacaagg tagtctcagc atataaaata tcaacaaatg aatgtaagaa agagaatgag
130141 aaagaattag tattagaata tcatagaaaa aatcatcttt gtgaaagaaa actctgatga
130201 aactggtgac ataaatactg agtaaaaaac agaagaccaa tcaaggcatt tgaggcttga
130261 acaggcatta caaatcatca agttgaatat ataagtatgg ccacttagtt attagtggtg
130321 aagactacgg tatttgttag aagaaataaa gtagatgatg aattctatag aatattctgg
130381 agaaagtgga ggtcttagct acatgacatc gtgtggctca ctttgggaac catgtttcag
130441 tttgtttagg tctagaaatt ccttggtggt ttctgtgttc agttttgctt taataatccc
130501 taaagatatt ttttaaaata caaatttttt agctttcctg tattgtacag caccaacgct
130561 tgcctgagtg tagtgagatt cacagagttt ggaaatttcc tgtagcatga ggaggctgcg
130621 gggcagcctt ctgtcatcta tgtgtaccca gcagtgtgct gtcctcacac aactcacgga
130681 taagcagaaa gcatctctat tacatgaaaa atttagctaa atatacaaat atccatgcac
130741 acacatcatg agagatggaa ataggctatc aaaagagtca gacatattga ccgtcttcac
130801 tgttagctgt aggattgctg cccaaccccc tgacacaccc acacaaactc acatacacaS
130861 aaatgaaaag cacagtggaa agcagaatag cagtctttcc aaacagattg aaaaacaaat
130921 gatgtctaaa gttcaatcct gacactgtgc tgattgcttg gacacagttc cgtcaaaaca
130981 tttgtccaag tcatagttgt accttttaga aattagccac aaacaacatc ctccccaacc
131041 cttcctgcta ggctagagtc ccagaagaaa tgagggatac acttgacctg aagtaagcaa
131101 agcagaaccc agtctctgag gcgaggaggc ccacctggta gggagctcaa atgcgccatt
131161 gtcctgcttg tctttataaa gggagctgac acgtttctcc cagcataaag ttgggagtga
131221 caccagagcc ttctgcaaga tgcttctgat tctgctgtca gtggccctgc tggccttcag
131281 ctcagctcag gacttagatg aaggtaagcc gaattggggg aagatattgt gactctgatt
131341 ggggtttacg ggcgaatgct atagaggggg aaagtggagg gaagagagga ggatgagaaa
131401 acagatagga ctgaagagtt ctcatgccaa ggatcagaag acctgttgtg ccttcattcc
131461 tcatcaagac ctcataattt attgattgca caaatagaac ccaataaaga atttgtaccg
131521 ggggagtgag agagtgagat ttgcatttat agagacatgg gactgctggg aaggatatgg
131581 agaatgcaag acagattcag ggaagtgcag ctgtgaagat cctatactga tcccagtaga
131641 cagggatgat ggtggccttg ctggacagtg gatgagtatc catgaaggag ataaacacat
131701 gtcagagcta ttgctgaggc agagaattgg gtaaacactt gcctctgtct acatagagtt
131761 agagaatcac cagagtgaaa tattgtcatt tttttctctc ctgcatgtag tattttaatg
131821 tgctgggacg ggcatttgta agattgtatc taagtggcta tgtctggtgg ctcctgttga
131881 gaaagcttgc aaacataaac aacatattta cagatgaaag agggcagaag gatccccaaa
131941 tatttcattg aaatactcaa gagcccttta actaaataag cactaaggct taaggaatca
132001 tgagaggaca aacaggggcc cttctatgtt gagttcctgg ttgacgctca gtgtagtaac
132061 aatcctgctt tcccttacat cttcttccac ttccggtagc atcagagagt ggctgatgag
132121 atctcaaagg ggatgcacag ggtgtgatca gaggtccttt atcctcgtag aacactatga
132181 gctctgaatg attcatgcag taacttttcc catcatcctg tacttctttt ctagatgtca
132241 gccaagaaga cgttcccttg gtaatatcag gtaaatccca ataaattctc agtaaactct
132301 gtctccattt ttccctgaaa aattgatcag ttctccagtg tcttcttatc atccttgtca
132361 ggaattggct aatatcagtg ccccagagat ataaacagtt ttctcccaac cttgattctg
132421 gggaccatga gtaaagaaat ttgatttttc accaccctaa tgtggattaa gaggagttct
132481 aattaggaag ccttgggaag gggggaggtt gggagttgag aggcaggtca gggagagagg
132541 ggccggccgt gtggtgaaga cagagagata tgaagacagg agggttttcc agcatgagct
132601 cagctcttct tgtttcaact cacacagatg gaggagactc tgagcagttc atagatgagg
132661 agcgtcaggg accacctttg ggaggacagc aatctcaacc ctctgctggt gatgggaacc
132721 agaatgatgg ccctcagcag ggaccacccc aacaaggagg ccagcagcaa caaggtccac
132781 cacctcctca gggaaagcca caaggaccac cccaRcaggg aggccatccc cctcctcctc
132841 aaggaaggcc acaaggacca ccccaacagg gaggccatcc ccgtcctcct cgaggaaggc
132901 cacaaggacc accccaacag ggaggccatc agcaaggtcc tcccccacct cctcctggaa
132961 agccccaggg accacctccc caagggggcc gcccacaagg acctccacag gggcagtctc
133021 ctcagtaatc taggattcaa tgataggtat gattccagtt tattatccat caaaggctcc
133081 aactgctaca gttctccaac ttcattgtgc cagtgaatct attgaaaagc tgttaatatt
133141 tccgtgtcct ggaacacatt tctcatgagt tttgttcaaa tattctggga taaggtagca
133201 agatcttgtt tttaaacaat ctcttgaagg caattccaat tttgagaatc actatcttca
133261 aattacctct cttaaatagg gttgggaatg aggacataga atcatgttct ccctttggca
133321 ctctgtttcc tttcctcaaa ctcaaagact cccatttatt taaagtttta cctgaacact
133381 ccttgttcag gacaggctca gtcctgcctc acactagcat ttcaagtcca gtattcctgc
133441 taaatggtcc ttggactttt agctgttaaa tggtatctca ttttttaaaa cacatttcac
133501 atttaaagtc atacatgctt aagctaacaa aaactaatct cactgaatag acatgtacca
133561 agctaaatag caattcattt ctcctcccct ctacccttac caaaactccc actttttact
133621 gtttggaaac tcctctttga aatatttatt gctacataac tatatataat tttcccacca
133681 ctaataccac actttatatt cagttctgtg tcttacttat tggaaaaatt tatttcttag
133741 ctttcacatg agtttattta gatctcttca gtgtttaYtg gttagttttt ttttttacaa
133801 ttatatatga tgctattgtt tggtttttcc ataatttact taaccaatcc ttgtcactga
133861 acattaaggc tgctttcaca ctgtccctat tacaggatat gttgcagtcc ccatctttct
133921 gaaaataacc cttaacgtat ccaacagcca caaagcatga acaacctaac aataatcgtt
133981 tttcgtcctc atctaagcaa

Following is a genomic nucleotide sequence of a GRID2 region (SEQ ID NO: 3).

>4:94744101-94841750
1     tcaactcctg ggctcaagca atcctcccac ctaagactct aaaataactg ggactaccac
61    ccagtcaaat gccaccacat ttgactaatt tttataaatg ttttgtagag atagggtttt
121   gatatgttgc ccaaactagt cttgaatttg gggcctcgag tgatcctccc acctcaacct
181   cccaaagcca tgggattcca ggtgtRagcc actgtaccta accctcttct ggaaaatctt
241   aaRgccttag attgcattcc tgtctttata ggtcatattt ctcattattc tcttcttttt
301   gaccttttct ttgcaacttg agagttttaa aagttcactt attgtgtaac ttattcatta
361   gtcctgatat atcatttgca ttctttacta ctcccaactg ttatttttat tctgcagtag
421   aagtttgtct ttattctttt ttgttttctt ttctcatatt atctcatctt ttatatcatt
481   accttccttt cagcctattc tctttgttgg ttctctactg atgtttcacc tagattttca
541   ttgtttaaag gcattcatca gattatattg caacattttt attgaatgca gccagaattt
601   atgtgaggta tgtactacct ctgagacttg agggtgatat tctgatttgt ttagggtgca
661   atgagttttt gtgtttgttt atgtttctat aaaccatcca ggcccaatct ttatttttgt
721   caatagttaa agaaagtgaa gcatcttttg cctcctctac aatccattta gttaagttca
781   gtttttcttt ctcagaccta gagttaaaga tcaaatagat atgttcagca tttaatcaaa
841   tttaccatgc atagtaggta ttccatgtat aggtttttga cagagattta ctaaagttat
901   ttggtctaat tatgacagag catttattcc agacactgtc ttgtttatac tacttatcac
961   tcagtgacta aatcttccaa aacaaaaatt attgtcatct caccctctct acttccataa
1021  ccttacggtg gtttctgctt tgagtagtag tctctggata tttgtgaaag agtgtaactg
1081  agaaggcagc aggtagtgaa taagagaaca aagtctctgg atactaataa agatagttct
1141  catatagttc aggatagctt gctgcttact tggccataaa gctcttacag cctaaagtta
1201  ggcataatca aaggaaatat tgttgcggga agtcagggac cccaaatgga gggactggct
1261  gaagccatgg cagaagaaca tggattgtga agatttcatg gacatttatt agttccccaa
1321  attaatactt ttataatttc ttatgcctgt ctttactgca atctctaaac ataaattgta
1381  aagatttcat ggacactaac cacttcccca atcaataccc ttgtgatttc ctatgcctgt
1441  ctttacttta atctcttaat cctgtcagct gaggaggatg tatattgcct caggaccctg
1501  taataattgc attaactgca caaactgtac agcatgtgtg tttgagcaat atgaaatgtg
1561  ggcaccttaa aaaaagaaca ggataacagc aattgttcag ggaataagag agataacctt
1621  aaactctgac tgcctgtgag ccgggtggaa cagagccata tttctcttct ttgaaaagca
1681  aatgggagaa ataatgctga attctttttc tcagcatgga acatccctga gaaagagaat
1741  gcgcacctgg ggataggtct ctaaactggc acccctgggc atggtcatct cttatggtcg
1801  agactgcaga ggtgagatag actccagtct cctatagcac tcccaggctt attaggaaga
1861  ggaaattccc gcctaataaa ttttggtcac actggttgat ctctcaaaac cctgtctcct
1921  gataagatgt tgtcaatgac aatggtgcct gaaacttcat tagcaatttt aatttcacct
1981  gggtcctgtg gtcttgtgat cttgccctgc ctccgcttgc cttgtgatag tctattacct
2041  tgtaaagtac ttgatgtctc tgacccacac ctattcacac actccctccc cttttgaaaa
2101  tccctaataa aaacttactg gtttttgtgg cttgtggggc atcatagaac ctaccgacat
2161  gtgatgtcta ccccggacgc ccggctttaa aatttctctc ttttgtactc tgtcccttta
2221  tttctcaagc tggccgacgc ttaaggaaaa tagaaaagaa cctacatgaa tatcggggca
2281  attccccgat aaaatatatt atatagggag actcaaagca tatttctacc ttccctgtac
2341  taatttgaaa aagaatatat atggttgata aatatatata tatatttaga ataagtatta
2401  agccccaatg ttatgcatac tcaaaggaat tatacaacat aaagagaatc aaagtatatt
2461  tctactYtcc ttggactaat tcgtcctgtt ctggttgtaa gtatgggata tgaaatacat
2521  agatgaaatg tatgcttttg aatctatccc caatctcaat ttagttgttt caaattattt
2581  gagaatttct catttatctt tctctaactt gaattatgga gcctgtggct ttattttttt
2641  ttttttaagt tttttttttt ttggtcagtt gttgaaattt ttaaacagat atttggagga
2701  ggtgtatcag agccagctag ttttaagtct cagactggtc actcttaaaa taatcccacc
2761  cagtcatgcc atcatcaaat ttagttgcct ctctccttgg cttaaattta atacattttt
2821  tccccaatct attggactct ttagaaatgt tttaaacaaa aatgactaat ttatttttta
2881  aatgtaatta attttgttga ttgaataaga atgtttaaaa atgaaacatt attgtgaaaa
2941  ttaccacatc cttggtttaa aatagcctga gaaaggaaag cacagccata agaaataaat
3001  atacgaatag gttaatacag aggaaattgt gccagacaaa gctggttgct tttttgatag
3061  ctttacagcc ttagtaaatg aacagagtgg agtagacata atgcatcttg acttctagca
3121  aagtactgga gacaatgtcg tgtgaaattt tgattgcaaa attaatttga gttggcttga
3181  aaaacactgt taaaggaaat ataagctggt tgaaaagaca tacagagaag agtgttgatt
3241  aatgtcagtg aactaatgag ttgagtgata tctccagtta aatgtcacga ttgtgagtgt
3301  tggattgaat tatttttgtt atctgttgaa aaaataaata acatagggtt gattaaatta
3361  gagcagaaaa tttatcagga gacaccagtg attacagaaa atctatataa agacatgaat
3421  tcatttcaga gaaaaaccag tgaaacaata taattttact taaagagtcc atttttttaa
3481  tcacaaaatg gaattctatg aagtatcaat gcttaaagac cttcaaacta cagaatttct
3541  caaaactaaY tggaaaacaa aattaactat ctcagtaagg ctagaacatc attttaatcc
3601  agttagtagc tacattttat cccaaccaat aaagtgacag ttaattctta cctagaggat
3661  gtggttaaat gtataattat tatttaccag aaataaaaaa agtatctaga aaatcagtaa
3721  cctctagaaa ctcttgggca caggagaggg agaaaactat ggaaggggaa gtaattatgt
3781  tcttaactat gttacattct taagttacat tcttaactat ggggatatag taagactcta
3841  aataactaaa gttttttttt attatacttt aagttttagg gtacatgtgc acaacatgca
3901  ggtttgttac atatgtatac atgtgctatg ttggtgtact gcacccatta actcatcatt
3961  tacattagat atatgtccta atgctatctc tccccactcc cacctctccc cacaacaggc
4021  cccggtgtgt gatgttcccc ttcctgtgtc catgttattt ttgaaatatc cagattaaat
4081  gtagttaagt aaaacaataa actttttcaa tgtaggtttt ttaatgctaa tgaaaagcta
4141  gtcagcatgg ggtaaattag attaaattag attaggtaaa ttggaataag aatcaaatga
4201  tcacaatcat aaaatgggat tagtcttact ataagtcaca cctgcaaaag gaagaagata
4261  acctggaaaa atttaaagag tcttccactt tacgaagtcg tagtgagttg gcaagtttta
4321  ataatttaaa agaaaacgtt gatttcatct aaaaagagag agagaaagtt ttagttattg
4381  attcaacatg tatttattga ataacttaaa tgtgcactat ttttggtttt gtgagcagtc
4441  ccaaacgatt aaaacagagt gacttgttcc ctgactatct taaattatcg caaactgatt
4501  ggcttaaaac aacggaaatt tattctctca cagttttgga ggccaaaagt tcacaagcaa
4561  ggtatcagca gggctgcact ccccctaaag gctttggggg agaatgattt tgagcgtttc
4621  agcttctgtt agctgtcagc attctttgat ttgtggcaac atcactccaa tctctgcctc
4681  catggccatg ttgcctcctc ttctgtctga caaatctccc tctaccatag tcttaaacaa
4741  acagttgtca ttgggtttag tgcatattca tgtaatctag aacactctcc cctctcaata
4801  cttaacataa ttatatctgc aaagatcctt tttccaaata aagcaacgtt tgtttccaaa
4861  ttttaggatt taatatctct gggtgaccat gatttaacct actacaaaag cattttctaa
4921  caagcagctt aaaataggtt tcttttttct ttgagacagg gtcttgctct gttgtcaagg
4981  ctggagtgca gtgSacagtc atggctcact gcagcatcac actcccagtt tcaagcgatc
5041  tttccacctc agcctcccaa gtagtttaga ggacaggtgc atgccaccac acacagctaa
5101  tatttcattt ttttgtagag acagggtctc tctgtgttga ccaggctggt ctaaaactcc
5161  tgagatMtag caatcctcct gccttggcct ccYaaactgc tgggattaca ggtataagcc
5221  actgtgccag gctcaaaatt aggtttctat tgctgcatta atatatacac agRgccatct
5281  ctttctcttc taattatttt acaaatataa agagcatctg taacacaatg aagctgtttt
5341  ccactttagg aaaagacagt tttgaaaatg tgattaatta tttgttgcag gttaggcttc
5401  atacagtcat attcccagtg cacattgtcc tcaagatctc cccagtttgg cactgaagcc
5461  ttattgactc tgtctctgaa agaggcctta aatgatcccc cttccttcta atcctcactg
5521  aaattccctt aggttaagcc caatttatcc aagggctatg gtcttctact tgatgttctg
5581  tttttaatcc ttgatgctat ccttctcctt ccatacaaac agtagataca catattttca
5641  aaacacagac tttcttgtgt tctctactta aaaatctgtc tagctttcag atacataaag
5701  gaaaagcaac ctggttttaa gagcatgcaa tgYttcttct taaatttccg atccctgtcc
5761  ttgtctgcag atttatcact taacattcct ctccttgctt cataatacta cacacacaga
5821  catacacaca cacaaacaca cacaaacaca cacacacaaa cacacacaaa cacgcacaca
5881  caggcacaca cacacacgca cacacaaaca cgcacacaga gacacacaca cacacgcaca
5941  cacacacaca cgcacacaca gttccttgat attgcagtca cacagaattg ttcactgttt
6001  cttgaaaata atcataaact gcatttgctc atgttatttt ttgagatcag agtgttcttc
6061  cctctttttt ctgctttaag gttttctctt tttttgcaca gctattaaaa cttggccaac
6121  tattatattt tctctatagt tttccctgat ccttacacat cttcatagct ccttacatgt
6181  aactctatta ctgtgtgcat catatgcgat cgtgtttatt ttccctgtgt gatttgtttt
6241  ttagactatg accaccttga agacagaact gggtcttatc tatctttaca tctcctagtt
6301  gtttgcatgg ttcttgacac atagcaaatg ctcataaact tctgatgaac taaattactg
6361  taaattaaag tcattgatac atttaaatgg cacgatttac ctatatctgt tttataatca
6421  tagttaacaa attaagcaaa atacttctag gaccaggaac tcaatatgga gacactccat
6481  tgtaagagag aaagatgcat tgaatcgttt attcagaaag aacttattat gtcactgtag
6541  gcactgcaca aaggctaaaa atcccaaaat agaattgtta aaaaattagg gtttaagatt
6601  aaaggcaaag gaaatcatat ttgttgagac tattagggtt gcttgaagta aactaatgcc
6661  aagtttaagt aaaaagattc ctgctatatg atataattgt taagcctaag agaccagcag
6721  gcaccaggta cagttacatt cagatgctca agcaatgcca ttagggaact gcccatgttt
6781  agccattact gtgttctgtt attccttttt tttttgtttt gttttccctc tcaggcagat
6841  tctactcatg tggtaacaag actattattc tagatactgt actcatattt aacagtctca
6901  gggaaagagt gaagcctttt actcaatcgt tccagaaaat atctaatgga tgaagggaag
6961  gctctaactg gccttcgttg gaccacttac atttctttga accaattact ctggtcagaa
7021  gatgaacatg tctgatcttt caggattaga ttcacatgca aaataatgtt acaattcaaa
7081  ctaggaaaaa taaattatat atttttaaga gactggatga aaatactcca tacatcacat
7141  gtgcttttct ttttctgtat attggtatat acataatcac tatttctttt tttttttttt
7201  tttttttttg gagatggagt cttgctgtgt tgcccaggct ggagcgcagt ggcatgatct
7261  tggctcactg caacttccgc ctccaaggtt caagtgattc ttctgcctca gcctcctgag
7321  tagctgggat tacaggcacc caccaccaca cccggctaat ttttgtattc ttagtagaga
7381  cggggtttta ccatgttggc caggctggtc tcaaactctt gacctcatga tctgccagcc
7441  tcggcctccc aaagtgctgg gattacaggc gtgagccact gcccctgacc caccactatt
7501  tcttttgtaa ttaaaaagta catatacact ttatattttg atgtagatgt aagaatagcc
7561  tctgcttcaa agacctcatt ataatagtat taaagacaca caagtaaaca aaggtaacaa
7621  taaaatagta atacatatca aatattaagt atctcaggaa taattattcc ttctaaggaa
7681  tggcaaagaa atttttcaca ccacaaagtt aattttgaat tgggtcttca aggaacataa
7741  caatttgaaa agacagagag gactttccag gaaaatgtaa ctacatatgc acaaatgaca
7801  gtagggatta tagtaaKWac acttttatta ttattctacc tcttcactct attcaatttc
7861  cctgactact acaatgtccc catgttggtt tctcctttct tgtattccaa gatattgtgt
7921  cctgcttttg cttattattc tctacccctt aagcttctca ttgcttcctc cttcttttct
7981  cttttttctg ggcctccctc tttgttttta cgatccctgg gaggactcat ttaattgact
8041  cctaaatggt ttctgtgtct ctagtgtctt gtagacatta gaggcacaga aaccattttt
8101  cactgtctat tttccactcg gcatccaaac taattttcca taaacattgc ttccatcatg
8161  gatatgtgct ttaatagcat tggaatcaga cagatcttaa gcacaataca atagtatata
8221  taccagatac cagttatatg actgtgagaa gatagtaact tctgagagtc tcactactaa
8281  tgcccaactt gtaagataat tatgaaaata tgtacaacat atgtatcact ctcagcagtg
8341  ctagccacta gatagatgtg caatatataa gtagataata cttttgtggg tatctatatt
8401  actcctttgc ccataaccaa cagtgaacca tcatgttcct ttttaagctt ggtggtggat
8461  acaggtgttc actttaaaag tctcagtctc agatttcaag tcttcaaccc atctcctaag
8521  ctgtcagagg cctttacaat ctttacctac ctcctttatt cagtcttatt tcctatcatt
8581  tccccacagg aactatttgc tggcccttta aKttctccct tacctcagaa cttgctaata
8641  tttatcactc ttaaatatat gtttttgctt gtgctgttcc tcaaactaga atttttctgc
8701  catcttttgt tatcctctct cttcttttag tttaaatacc ttctgttctc taagtttttt
8761  ttttccagga caacctagaa aaaaatatct ctttagtttc tacagtggca gaaggtaatg
8821  tgatttacca atttaattac ttcatattac aatatagtga ccatttatat cagtgtatag
8881  tacaaatatg tatactgtgg tcttactact gattatctta ttcttattta ttgcctctcc
8941  aacaagatgt tatcacccat agacaagaat catattttat atatctttat aacccaaagt
9001  gcctttttaa aattaatgta tcatgtttgt tttcctggaa caggaactga aatagagagt
9061  atgaattcat ttgtaactga atctttgctg taatagttat ccccagcatc cttgctaata
9121  cagagaatga agccttaata cctaaagcag ccactgtRtg tgattaatta acttctctcc
9181  tatgcatctt gacaggtagt ggctatgtgc cattcttggg agaactggga aaatggtcac
9241  tgaaatcatt tttgttgttt ctagttccaa tgagaaatgc cagcYgggaa aggctgctac
9301  agtatgtagc aaggtgcttt tgctgaaata ataaaagtat ttgcaaggaa atacttgaaa
9361  taagtgctaa atttccaaag attgatctac actacagaag gcttattagg gtatattgat
9421  actagtatgt gttgtcttaa gtggtacatt gtagtttcca tgccaattct tggtttaaaa
9481  cgcagcatgc atctgttata tgccactttc cacttaaatg cctaagacag gtagattgca
9541  ctgtcaagtt aggtcattat aactccaaca gatgttgtca tgtagatgaa gaaaattcat
9601  tttaaatgat atcatttata aaatgtgttt ttctttgtac ctaaaaaagg caataatatg
9661  tcatattagc tcttcagatt caggttgcaa tcagtatggc atgttttgat taaacacttc
9721  acaatttcaa aatcagtgca ggataatgat ggagcacatg tgaggaaaca gcaatagcag
9781  gggtgtttac agtatcccag ttgtttcaag cttaataaca cttcatgtta tcaatcagtt
9841  acaaaggaat aattggtgga taggctgagg aaatggtaca gtaagaaatt gtacttattc
9901  taaactatag ggatggaagt gcagccaggt caatacttat ttggttagta tattgtaact
9961  aatcatttga gttttgccag ctggtatctt atttttgctt atttgtttaa ccgcttttaa
10021 ataagtatat aattttaaca atttaagtca gttgaaatcc cccccataga gctctcctcc
10061 aaatgcatcc ctagctacat tcttctttct ggagattaac cagaaattgg tggtttcatc
10141 ctgtttatat gaatacattc ctagaagata aaatcatatt gtagatattt gtgacaaaat
10201 tttcctcaca actatatgaa agagaacgaa ggaaggtgtg tggttaggag ttagctgtca
10261 ttaaaagtgt gtaaattttg cacgttccct acatttattt tccagtaaat tttactagtt
10321 tctttctaat cagttgggac taaagtcaat cgacctgtct ctgcttcttt caatttcatt
10381 tcccaaaaga attttagtgc ttctctatta ttcttaactc tgccttgccc caatacatta
10441 atcaatgctt tgggtaacaa cattagattt gtacagggaa ggaccaggga atggcattcc
10501 aaagcaacac atggaaaggg caagagctga atttcataaa ccgtaaccta atccatcact
10561 gcagctttcc gttgcctcta cccagactgc agccaaacat actctaagct gccactttag
10621 agtatcttta ccctccatgg gagtttttct ttttttccct tttttttttt ttttttgagR
10681 cagaatctca ctctatcgcc caggctgaag tgcagtacag gatcttgtct cgctgcaagc
10741 tctgcctccc gggttcaagt gattctcctg cctcagcctc ccgagtagct gggattacag
10801 gcactcacca ccacgcccgg ctaatttttg tatttttagt agagatgggg tttcaccatg
10861 ttggccaggc tggtcttgaa ctcctgacct cataatctgc ccacttcatt ttcccaaagt
10921 gctgggatta caggtgtgag ccaccgcgcc tggccaggag tttttcactg tgatgttgtg
10981 taggtctctg gaatgtatgt ttccctctgc agtttcccca gtgttgattt taagggagga
11041 agccagagca cttgctattt caccatcaca cttgtttgaa gtgtgagtta ccatgcaatg
11101 tttaaaacat gagttacacc tttttggagg atgctgtaga ataccgattt attttttgtg
11161 tgaaaacaaa ctctacctca ggttaatcaa ataattactg aagaaaaggt ttttctttat
11221 tcaaatattt cagcacatga atgaagaaga aatggtaaaa gtaaaacatg aacatttggc
11281 catcactaat tatattagtt tgtagaggct actgtaacaa aattccacag accaagtggc
11341 tgaaacgaca aaaatttgac tgggagttca aaagcaaggt gccagtggat tgggttttcc
11401 ctgaggcctc tctcttcagc ttgcagatgg ccaacttttc cctgtgtcct aacatggtct
11461 tctttgcacc cgatggctct ctttatggtc taatctcctc ttcctgtaac tacaacagtc
11521 tgattagttt tgggcccacc catatgatct cacttaacca taattatctt ctttaaagac
11581 cctatcttca aatgcattgt gaggtactgg gggttagggc gtcaacacct aaatttgggg
11641 aaacacaatt cagctcatta aatgaatgga agtcatacct gaaatcaatg acggatcgta
11701 tgactacaaa aaaaggagac aaacattata cacctgctgt ttaaagtata caacacaaaa
11761 ttattgacaa atattaagct gaattaggtc aatcttctag atttaccaat ttgcaaataa
11821 tgcaatccac agagcaagat gtggatatgg gggattcaga cagcaYaatc tagctcggga
11881 aactacagaa aaaaaaaatg acccattttg tgacatataa ttgaaggaac aaatgaagat
11941 agatgaatgg agaacccRta aataaagcaa ttcaagatac acatgaactc ataaaacata
12001 ggtcctaatt tgagtaaaga aagaaaattt taatactgac tggWtatttg atgacactga
12061 gaaactattc taactgtgca gctatgaaaa ggaatgagat catgtccttt tcagggacgt
12121 ggatgaagct ggaagccatt atcctcagca aactaacaca gaaacagaaa accaaatgcc
12181 acatgttctc acttataagt gggagctgaa caacgagaac acatggacac agaaagggga
12241 acaacacaca ctggggcctt ccccggggbg gagtgcagag agggagagca ttaggaaaaa
12301 taactaatgc atactgggct taatacctgg gtgatgggtt gataagtgca gcaaaacacc
12361 tttgcacaca tttacctttg taacaaacct gcacatcctg cacatgtacc tgcacatgaa
12421 ctaaaaataa aaataaatta ttctaaatct tgggaagtat gataatggtg tcatgattat
12481 gttttaaagg acttcttaac ttttagagat atataccaga atagaaataa tattgtataa
12541 tgtctgccat ttgtttcaaa tcaaatctga ccaaagtagg ggcaagaaag agtaggcgca
12601 taaataaata actcattgat aatgagttga taattgtttc agtaggatga tgaatatttg
12661 agtttataat tttattctat ttatatattt gaaaattttt atactgaaaa gttgaaaaag
12721 ctttatattg gtattttaaa ctttgtaaat gtKcactaaa agaagatatg tctttttagt
12781 tgttttgcct gagttatgat gagctttgat gagtcaatca ggctgcctct tgagttttcc
12841 ttgtaaggtt aattgggtgc actggagagc tagctgaggt taggagagca ggctctggtt
12901 gaaggacagg gtttcggctt ggcagactaa ctaccttctc tgattattaa actttgatgc
12961 cacccacatt agcacagtat tctagtcacc tgtgataacc tgtccagaag ttgctctgat
13021 cgtgttcagc tcaataagtt tagacttcac acaatagtgc ttgtcatttt ttaccctctt
13081 gtaccaaata tatttcaaaa tatgtccaat ccagctgtta atttgactga ataaaatatt
13141 ataataacac ttctgaggat tgagatgatg tttgaagttt ttcaagagtc ttttccctct
13201 ggattagaga acatctctgc cagaatcaaa atgagtctca aaattgacag tttttacttt
13261 acttgctaca gtaacctggc attttaaaaa gcattctgga aaaaaaaaat gaatcttctg
13321 attgtgtaat aaaagaataa gaatagaaac taagataaaa tgattctaca tgtatatctt
13381 gacttatgtc tatattttat aatttcaact tttacgtcaa aaaattgtaa aatgtcacta
13441 attttataag gtttaattta acagatcaaa gctcatagga ctttgttgaa aacaatgaat
13501 gcaaatcctc agtacagttc caggtgtgtc atgagccctc caaaaatgat agttacaatg
13561 actgtttcaa aaaatttata ccttcaacaa aactttaatc ctatttttat atgcctgtct
13621 gctctgaaaa taatttaata tgtttaaatt cctagaataa atcttacttt aaccaaatta
13681 ataattctta aacttaaatt tagatttcaa tgaatcccca aaatggtttc cacataaagt
13741 aatgcagcta atatcagcac tgctaacaga ttacacagaa aggtctatta taatgattga
13801 gtaaatattc ctgggaaatc aaccttgtta aatctatatc ctataaggaa aggtgcatat
13861 ctgttttttc tagtaaagat tccgtaattg ttattacaag ttaatcagct tctcccaggt
13921 tcatttactc tggattttat taatttatta caggaaatta ttgggtaagt aaaccacaat
13981 tataataaaa tctcatacat tttattatat ggttcacaaa ctttataaac acctgtgatg
14041 ggattctgct attgatattt aagtggatat ttattaagga actagagata cttctcagta
14101 agattagctt agagaacggt cctgtttttg atctcttaag attccaaatt tttattccaa
14161 attttctgat attgcccaag tgtcttttct gaatctatgt ctttattatt tggcttaaaa
14221 cctttgtgca tctggtgctc tattctgctg tgactttgga gaaaggttac agtttgtttt
14281 tgctgggagg aataacttct tgaatgatgt cccaactgaa aattattttt ttggttggat
14341 agaacatccc tgtacaaaac ttcctctatt atcccttgga taattcctgc tgtatataga
14401 ggtccaagat tgattaatga ttttatcttc agtaacctgt gatctaaatt accattggca
14461 agacaagagt gatacctgtt tttttattct ctacattaat aaaacatgcc ttaagaaatc
14521 tatatacttc atatagagga tatgatttgc cactaagcaa actctatgaa aaagaattgt
14581 gttttcatag taaggcttta ttataaacca aatatgacca actttcagct aaactgtatt
14641 atctaatcaa gtttattccg tcactttact tgctattatc aataagacaa gtaatatatt
14701 taaatataga ataagtaaca agcaacatgt taactgaatt aatatattgg taaaatatag
14761 attttaaaag ttttttgaaa atatttgagg agggttgcac ttttgctccc tctgatttta
14821 ctacgtatgt ctctgactgt atgtaatcac acctagctta tgctgtccct tccattctct
14881 acacttgact ttagaaagtc aaacgtagac tggagcaaaa aggggagaga tcttttccta
14941 ccatttagca gattaaaagg tagattggaa atgcatctca gtatctcagc cataaatgtc
15001 taagggataa taatcccgct acatatttat taaactaatt acaacatatt gcgcaaaata
15061 aaatactttg aatcagaatg acttatgaat ggtcgattgc atacaaaagt gctggagata
15121 tggtgataaa aagatgattc agccatcaca aagctcaaaa ttctactggg agaaacaggt
15181 aaagtgactg aagcccagga taatatttca aataataagt acagtgagat acttatttga
15241 gagatgcaaa ctcaacaaca gtttggtaag tactttctct attctaattt gaatacattc
15301 tagtcaattg agatacacaa agcatcaatt ctccccattg gatttggatc ccataaaact
15361 acccatttcc ccaaatagat agtgttttaa cttaacagac ctcataaaaa gcttgacact
15421 aaaatgagaa tttataaatt gtgatctgta gtaagttaaa tggtgcccaa ccctgcgaaa
15481 aacaaaaagt atgtccatga actaatccct ggaacatgta aatgtgatca aatagggaaa
15541 agatatcttt gcagatataa ttgtaaagaa tttcaagatg agatYgttat ggaatatctg
15601 agtgggctcc aaatctaatg gcaagtatct ttattagaga aagacatacg gggatttaaa
15661 acagaaaagg aagaggagac agccatgtga aaatggagac agagattgaa gttatgcagc
15721 cacaggtcaa agaatgcttg gaaccaagag aagctggaaa agacaatgaa agattttccc
15781 ctagagcctt tggatagagc cctgcaaata ctttgatttc atatctgtcc tctggaactt
15841 ggagagaata attttttgat gtttaagcag atgactttgg ggcagtttgt tatggcagcc
15901 attgaaagca agtcgtgatc tagtttgcct tattttccaa agcatagata attttcaatt
15961 tcaattagga aaattgaaat tccacaaaat gcaagtgcat acagaaatgt ctatctgagt
16021 tgcagttggg aaacattttg catttaaaaa ggaaaagttt taagatataa aagaaagtct
16081 tgagaaaaca agagaatatc cctcatatat gtacaacttc ttactaatag aagccttaga
16141 tgtctgatca atttcagagg caaaggctac cattgactgt tcattgacca ttgacaagcc
16201 atgtgtttca atgatgaaat accacataac aaaatactct aaagcatatt gtcttaaaac
16261 aactgtttta ttactaaggt tcctgcggat ctgatattca ggcaggattc agtggggata
16321 gcttacctct gttctacatg gcgctcactg ggatagttca attagggtaa gaggattgaa
16381 gatggtatca tttgcatgtc tggggctttg gtcctggctg ttggctgagg tgctttagtt
16441 acccttttct ctccacatag cctctaatta ctcaatattc taccccagcc tttaaaaaaa
16501 attttattgt ggtaaaatac acatataaaa tataccatct taaacatttt aagtgtacag
16561 ttcaatgata ataagtacat ttatattcct ttgcatccat catcacccat ctattttcaa
16621 aactcctttc atcatgcaaa actgaaactc tatacttagt aaacaatgat tccccatttc
16681 cctctctgtc cagcccgtgg caactgctat tctttctgtt tctatgattt tgaccatttc
16741 aagaaactta tgttaagtgg aatcataagg tatttgttat tttgtgactg attcatttca
16801 cttagcataa tgtcctcaag gttcatccat gttgtagtat ttctatcctt tgtaaggctg
16861 actgataagg agagacttac ttctgtcatt taaaaacttg ttttctatat ggcttatagc
16921 atttttgtcc cttgcttcct gcgtgactgt cttcttttgt gtttagttga ttattgtaat
16981 gaaatgttta tgtatctatg tcattgactt ttgtatattt tctatagcta ttacatttaa
17041 catcctacag ttataacact cttatttaga tttatgccag tttaactcca ataatacaca
17101 aaactctctc ttttacattt ccatacccac ttcttttggt tgctgatgtc ctaaaactac
17161 acctttacac attgcaggtc caaaaacaaa aactaataat ttttaaatat atcagtttct
17221 tgaattaaac cattgttaca ataatagtag cttttataat tgtccatata cttaccttta
17341 atcttcagaa ctcacattaa cgtttcttcc agacaagtct agtgttaaca aactcctctc
17401 aacttttgta tacctagaaa tgccttaatg tctcccttag tttgaaggaa atttttgcca
17461 gatataggat tcttgcttga caggtttatt ttctcttagc actttgtata tattggccca
17521 tttctttatg gtttccaaaa tttctgataa gatatatgct tataatttta tcaaaaatcc
17581 cttgtatgtg attaatcact tctcttttga tgtttttgat attctctttt ttgtcttcat
17641 catttcaaag gttgataatg tggatctctg agctcattgt atttagagtt tactgagctt
17701 cttaaatgtt tatattcata ttctttgtta tatttgggag ttttggccat tatggcttca
17761 aatatcctct cttcttttct ctgtcttctt atggactcca taatgcatct gtttgtccac
17821 ttgatggtgt accaatggtt ccttattctc tgttcacttt tctttaatct tttttctttc
17881 tgttccttgg attgataatt cccatggtct tctctttaca tttgctgagt ctttcttctg
17941 gctgcttaaa tctacctttg aaaatctctg gcaaattttt ttcatttcac ttgtactttt
18001 tagctacaaa atttctttct acttttaagt tttctatctc tttatggacg tttataattt
18061 attcatgtat tatttcttga tttcctctac ttttctttta gttctttgag catctttatt
18121 acagttgttt tattacagtt gtccagtagg tcccccatca tgtctttttc agtgacagtt
18181 tctgttggct tattttattt tatttttaaa tgagccattc tttcctgttt ctttggatgc
18241 cttataattt ttttttgttg aaaattagac atgtgactgt agtaatgtag taactccggc
18301 aatcagattg tacttatttc tcctaggttt tcctaggaga aatttcctag gttttcccct
18361 tttgttctaa atgttgtaga ctagactgtc tctgtgcaaa ggatcaatct gagatgtacg
18421 taaatttaag gtcttctcaa gtctcttttg agcctgcacc tttccctggg catacacagc
18481 aactttctaa tttcccgtgt gtatgtggtt gcttttaaat gtcttagtat ttaatgtcta
18541 cctccaaaaa gaggaaaaga gaaaaatgaa ggtggcaagc aggcactggc catttaaatc
18601 ccctggaaat tacttcagcc agaggggtag gggtgtgcaa caatggaggg gaggtgcaac
18661 aacagtgact acctagccct tcgtctacaa gtccgtgatt agaagcagca gtaagcaatc
18721 agagcacaga tctccaatat ttggattata acattccttt tgcccatgct ggcttttgtg
18781 aactgcatgt aagttgctcc tgaaacatat tcatgNctgc ctgccattgg actaagggtc
18841 aggataggta gctgctactg ttttaagagc ttaaattgat caaaagtaac cataattttc
18901 cttataagct ttccactgga agtttcaagc cttcaataga ctccagaact ccagaatatt
18961 tatatcagat agattctgcc agtgcagttg ttgcctacat ggggagacag atttctggtg
19021 ctttctattt tgccagcttc ccaaaatctt cctttctaat cttgtttctc tccttgaaag
19081 ctggggagaa ccagctgtaa ttctaatttt tgttgttagt ggactatggc tgtgagaaat
19141 gtcctctgga tggggatgcc actaccccct acacagctca catctcactc acaactttgg
19201 ttttcccctg ctctggacca gccttttttt atttctttat ttctttttca cttgattggc
19261 ttccaaaaat gagaagagaa aacctgtgag atctcttaag attcatgcca ccaaatcata
19321 tgtcatcact tctgccacat tttactggag aaaactcaca gagcaaactc agattcaaga
19381 aaaggggctg tcttagacct tttgagcttc tataataaaa taccttagtt tgggtaattc
19441 ataaatagta gtaatttatt tctcagagtt ctagaggctg agaagtcaag gcatcagcag
19501 attcaatacc tggtgaaggc ttgctctctg cttcgtagac ggcacatctt gcagaagggg
19561 tgaacagtat cctcacatgg ccaaagggga aggcaacttc cttcagttca ttttataagg
19621 gagctaatcc tattcatgag ggaagaaccc tcgtgaatta atcaccttct taaatcttta
19681 atacaattga gttccaacat atgaattttg gagcgcaaca atattaggga agtggactcc
19741 atctcttgaa agaagaatgg tatgtgtgca cagggatagg aggaattatc tgtggccatc
19801 ttgcaggcag actaccacac agtgctctgc tttttcttat cattttcttt gatgacatct
19861 gatcctttct cacaaatatc tcctttgtaa aacactcagc acttattatt tatatcagtc
19921 atttgggaat tagtcatatg ctgcctagtg gaatttgttg tcattaaaaa ttttttcctg
19981 ttttgtcatt tcaaaaacaa tcacaaactc ttggaggtta aagatgatgt tctatatttc
20041 cttgtacccc gtaaaaccag catagtaggt atttaataaa taactgtctt cttaattata
20101 aattttaagt ggtgggagaa tgatgtattt acactaatgc acggtggttg tattattatc
20161 cactagattg tttcttgctg catatcatgc actgcattat ttcttgctgc atatcaatca
20221 cttagtactg acctcctttt taaaatacgc ctgactttct catgcttatc ttacatatat
20281 ggttatagct aagagttgtg aatggacaca gatatgggaa gcatatttag gaggattaac
20341 aaaagagtaa ttagaagaac acataaatcc acaaggaaaa aatgactctt tttgaagtat
20401 gctagcattt tcaacatgcc tgttgagtta atgatttatg ggagtttcaa ttgtttaaat
20461 gtttggaata ctctgcaata aaataaaaat tctcttcaaa gttgataaaa atactcagag
20521 gcatacttcc tcatctgatg attcatagtt accaatgaaa cccaagaact ccatgcacat
20581 tttcagcaag agcaaggaat ctgatagcaa gagagctgga aaggaagaat catacagtga
20641 cctagacaag tgtcatgtcg gtcttagcat cagataaact gattgcaatg gttgattgta
20701 ctatctctag cgagagtaat ttagaaaacc tgaataagaa accctctctt gttcatcata
20761 gggatgactc ttggtgtgaa cccaatcata ttaaacaatt gtagaatcac caagctagac
20821 attttatgag actaactgat ttattcctca tcttgaaata aaacctgcat gccacaaaac
20881 tcctctatgc tgctgagact tagttctttc tttgagaaaa ataagagcca tttattcatg
20941 caataaattt tcattgtgtg tgaattgtat actaggcctg ttccatgtgc ttgccatacg
21001 tcagtgaata gaagaggcaa agatctttgc ctatggaact tgcagtctag taggggacat
21061 aagttatttc gtaagcttcc cctttataaa tatcattaat ccttactaat aataatactt
21121 acatcaatgt ttatatattt tacattggtt ttctgataat ataaaaaaaa attcacaaaa
21181 taaacccaac cactaataag ctgtgagttt tgagtgtaga attcttttca ttaacaaacc
21241 atttcatttt cagatcgaga aactaaggtt tttaaaaaac tgattttact gaaaataaaa
21301 tctaaccacc catcctaatg ttctttactt ctttatctgt ctttcaaaaa tatcttcctt
21361 tctctatgtt gagttagata tgtattcaca aaatctacca tactgtccat atatgttaga
21421 tataaatttt tgagctcatg tgaaagaaac caagtttgaa tagttgaagc aaaacagaag
21481 ggtttattat taaaaaaaaa atgtgttttc tcaaggaccc caaaacacaa agctgaactt
21541 gagaagtgag cgctcaaaac tattaaggct gtctgcatga cccttgctct aattcctctc
21601 tgaaatagtg tctcattgat tgaggtaaaa aatgacagca gtttctagag ctacactgcc
21661 tgtgttcaaa tcctagctat gtcatttgtt agctgagtag cctggaacag gtaaattaat
21721 ccctctaatt ctcaatttcc acatctgtta aaattagcat aattataatc atatcaggat
21781 aattctgaca ggattatagc aaaaaaagat taaataatat ctaatgactg gcacaatacc
21841 tgcctcataa atataagtct tcagtaaata taaaccacca ttattaaata ggagatgtta
21901 agaattgagc tgagctgggg agggctgagc tgccagatgt gggagtattc agtaagacaa
21961 caagccaaaa tgaaagtaac gattaagctt ttattccact tactgtgata gtataatcaa
22021 gagtccagaa agaaagtgcc agcttcacct gtgtttcatt ttccctacaa atggcaccac
22081 gcaagggtca gatgcattag tacagttttg gataactgtc ttactgctca gggaacccca
22141 aacaaaaagt tcctatggtt ttatggacct aagggcagga gaagaagcaa ggggaagatc
22201 taggaatggg aaagtaccaa gtactaagtc agaatggaga aaagtgtcct ccagggtacc
22261 cactcctccc aacaagcagg tcttggcaga ggctcctgga aaggttcatc tcagagtccc
22321 cagtaaagaa gcctctgaat ggaggctcct gggctaagaa tgcaaatatg tataaaacat
22381 ggctggccag gggtcctaag tccttgactg caactcccgt cagacactgc agcacattgt
22441 ctgtgcaagt cagtttgggg agggaagctt tcctctgtga aggcctccca ggcaaagcct
22501 ttgtattgcc tagggcaggg caaaatcata cagaggtttt tgacttggag ctggattcct
22561 tgagaggatt tctaggagaa atcctcccaa aagtttcaac agtttatatt tcatttgtat
22621 gttggtggtt ctctttcaag tgagatataa tatagatgaa aagtatctaa catagattag
22681 gtattactct tattattact atagctatgg ttgcttgtat ataactgttc agattatagc
22741 tccaataaaa caacttattt tattctctcc agagttaggt aatatcatta ttcctcatac
22801 tgaaggcttt tgaaaaatgt tccttggtct ttactataca gagtttaacc tttctgctct
22861 caggaagaaa gacagttgga aattcaccat ctaaatttaa aagggggaaa aatctcagct
22921 gatagctggt aacacagtgt tggctatgta ttgagatact tacttaaagc atcatataag
22981 aaatgatgta caggccaggc gcggtggctg atgcttgtaa tcccagcact ttgggaggcc
23041 aaggtgggtg gatcacctga ggtcaggagt ttgagaccag cctggccaac acagtggaac
23101 cccatctcta ctaaaaatac aaaaattagc caggtatggt ggtgtgtgcc tgtaatccca
23161 actactcggc aggctgaggc aagagaatcg cttgaacctg ggaggccgag gccgaagtga
23221 gccgagataa tgccactgca ctccagtctg ggcaacagag caagactctg tctcaaaaaa
23281 aaaaaaaaaa agaaagaaat gatgtacaat cactttaatg tgtattaagg ttcttgttta
23341 aaaatcatct aaatttgttt agtatccaat aaaaattgaa gtctcacttt gtgccaataa
23401 ttatgtattt aataaatggg aagaggaaac aaaacagtaa tttttcttga acacgtggaa
23461 atttatagat aattgctgca ataaatatta tcatacaaaa tatatattta aaaaacttaa
23521 ctacaatatt actgtaaaag agaggaacat gattctatat tacagtgata tgaggtagcc
23581 tggggtgggg gcaggagatc aaggagagtt taccccaaac tacaggtgtt tatattgaga
23641 tctaaaggat gaggaaaaat tactttacta attcattaaa gaattgcagg tgtcttaaga
23701 ttgttccagt caaggaaaac tgtatattca aaaccactga ttctcacatt tggctctact
23761 ttggaatcat cttcataact ttacacaata ctgatgccta gatttcatgc ccagattctg
23821 atttcattgg catggggtgt ggctttggtg tcgggatctt tttttataac tcccctagcg
23881 gttctgataa gctttagtca gtgctttaga aacaatgttt aaattttcaa ggtaggaggg
23941 ttatgcctta ctcaagaaac cgtaggaaga ctcatatgat ttgaattcaa agaatgagag
24001 ggaaggcatt atgacatgaa tYgatgaata taaataataa caaatcatgc aaattctggt
24061 aggccacatc caaagtttgg cctttatctt gagaatactg gaaaactatg gaaagacttt
24121 aagcagatgt tataatctca gaatttatgt ttgcaaagaa agactgtaaa gagaagctat
24181 gaaattagct tcggctagga tggtaggaag gaacgagtta aatagattca ggagacattt
24241 ttaaagaggt gaaattggca gagctgggca aaggattgga tctgagaggt ggtgagactg
24301 acagcttgat ttttggctag tgggactgag ctgatagtag ggatagatag tgagcttaat
24361 cttagagata tctgagctcc ctttgaggta tctaagcaga gacagtgaag tgatagtgaa
24421 atataaggag attgctgagc ttgagatttt tattgtttag gagtaaagat ctaaattgca
24481 atagagatgc tttacactaa ccttgtcagt ttcataaaaa ttactttact ggtacaaaac
24541 atggtaaaag gcactgtaat agacagaaaa atgattcaga tggtacccat gaccttaagt
24601 ggttaattat ttaatggagg agataggaaa gacagtgaga atttaatgtg attttaaaat
24661 gaacatctta aaatgtctca aggtagaatg gaatagaaga gaagaaaatc cagcaacttt
24721 tatcaggttg taataattaa atgctaaaat attattagaa aaaataataa tattgtgatc
24781 tgatagaaaa ggaaagctaa tagaatttga gggaggggaa tgaccccgaa gaatcatgaa
24841 aactctcagg gatacaacat catattagga cacggtgtct agttggaaac attttggttt
24901 ttcattcagc ttgaactgga gaaccaaaaa gaaagaagaa taaagaggac agcaggagtt
24961 ttaaatcatg gatttgttta ttcaaatctg ccaYgtaaca atacaacaat actatgataa
25021 gagctaggag tacaactgtg gacaaggtag tcatcattct ggcacatagt ttataggttc
25081 atatcatatt tattataaaa aatagtttac ttgagacaat atccctagtt agctttcaca
25141 aaggaaacca gtcaaaataa tgactttatt aaaagaggtt aagtagccat tctatgagtt
25201 tgcattccct gtgtgttgaa gcaagttttt ttttttcttt tttttttctt tttttgagac
25261 agtcttgctc tgttgcctag gctggagtgc agtggcaccc tcggctcacg gcaacctcca
25321 tctcccaggt tcaagcaatt ctcccgcctc agcccccaaa gtagctggga ttacaggcat
25381 gtgccaccac acctggctga tttttgtatt tttagtagag acggggtttc accatgttgg
25441 ccaggctggt cttgaactcc tgaccttagg tgatataccc gccttggcct cccaaagtgt
25501 tgggactaca ggcgtgagcc accgcaccca gcctgaagca agttttaggg cagtggtttt
25561 caaccttggc tatacattgg aattacctgg aaaggctttt aaactactga tgccttggtt
25621 atactcctgg ggattctgac ttaattgtcc tggggtttgg atggagcatc agaatttttt
25681 aaagctttcc attctaatag tcaacaaaat attctaatag tcaacaaaag ttgaaagcca
25741 cagttttaag ggcatgtttt atcactcaga ctactaagtc acccgaaaaa tgagactgag
25801 caaggaacaa aagaattctg attatatagg aaaaataata acaaacattt ctttagtctc
25861 agtgtgtcat gaacacttct aaacacttaa catattttaa atcagttagt ggtcccaaaa
25921 actgtatgag gaagtcactt ttactattcg catttgagag atgagaaaac tgaaacccaa
25981 cagcacacgc aaagtaagtg ttagaacagg tacttgaact caatcagtct tcttctagag
26041 cttcttatct gaactggtat gctatattgc ctctaaatgt agtgtaatgg taattttata
26101 ccagaagaat tataaaagaa aggaaaacct ataaaaatag gatatcatta aatcagttaa
26161 atgctagttg ataatagagt gattattatg actgcataaa acatctatgg gttatacata
26221 tgtgtatata gacatatata tgtatatatt tattcaaata tgtctatata tagatatatg
26281 tatacatatt tgtatatata tgtgtgtgta tatatatgct ggttctcatg ttttcttcat
26341 ttgtaggaat cagttaacaa gttcttgtta catagtatga aggttctgtt ttcagtagtg
26401 aaacagggag ataaaagtgg ttgaaccatg tgattttttt tctgactttt caaagacatt
26461 ttctcctgaa ggaagctcaa gtgctaaggc tattccacat aatctcaaga acgactacta
26521 ctttatagta atgaccaaac tgtagtaata gaatcttagg agcatataat catcctctag
26581 ggaacacatt ttacaaattg aacctatctt gcaggtggaa ataccataaa gaaaatKatc
26641 attggagaga ttactggcac actgcattca ttcacatgtc aaagtggaaa acattccaat
26701 ttacttctat aaaacatgta tctgagtcaa cctgccatat gtatttttta cactttctat
26761 ccccttacag aatatctacc aaataaatgc tgcacatttc taagtggctg gcttttaagc
26821 tgctttgcct atggcttttt gttccctctt tttctcagct gagaaatcaa gtaaacaaaa
26881 cattgtggct ggtagcatag gtcctcagtg cagtggatat tttgtgtccg gcagctggag
26941 cattggtcgt acagatgggc ttcatttgtt ccatatgaat acaagttaat aaatggagag
27001 aattccattt ggtattcaat taagatctcc cctgactcct gttattaaag cctacaaact
27061 attgagcata aaattaaaat cgagcagttc agctcttttt gacattataa cactgcctga
27121 aaaataatgt aaaacccaag agtcacaagt cattccttac agcccatggc aaataagaag
27181 tggaaacatt gaggaagaaa gagtaaatat ttaaaacttg atcattagta cacatgttag
27241 gagcaggcta ctgtgctcat gcaaatacat caatccagtt tgttgaacac aagatattac
27301 attaatttta acagaatgtt acaagattat tattgccaaa gctccaatga aggaaataat
27361 taaagcacta ggcattttaa ggtcttaaaa tgacagaaaa tactatagga aatcaataac
27421 cagaaaatac tatagaaaat caatatactg taattgaaag gaaattgaat tgggtttact
27481 ttttttatcc cgtctaaatt ttcttcagat tgccagacca ctgaagatag tgaatcttgt
27541 tttgccatca gcagtgtgac tttctctaag tctcatgtgc tgcaagggcc atctttattg
27601 ttttttattt aggaagcact cagctgaata gacaStatga gattttgtgt gtgtgttctc
27661 tttactagtc ttggactcat tgttaaaaaa aacagaaaca aaatcaaaga cacagcagac
27721 aaaaacaacc acctccacat aaccagagaa gtcaaattaa tgagaattca gacatcgaaa
27781 ccagaggact tgttagataa gcctcacact tgaacctaag tgaaattctt ctagttttta
27841 tcaaacacta tgcctgtcat tcttgctatg caataagaaa cattaccagt tgcagataca
27901 aattcaattt gaatctatat gaatctaact cattttcatt gcttattatt ttaaaaagtt
27961 attataatca aacaaaaaag cttgaaatga acctcaggga ggtaagtgga tatgaacaga
28021 aattaaagaa tgttaaaatg agaaatccag actgggtgct gtggcttaaa cctataatcc
28081 cagcattttg ggaggctgag gtgggagaat cccttgagcc caggagtata aaacaaaaca
28141 cttaacaatt ctgagtctca atgtcgttga ctataaagta gaaatatctt cccttcctac
28201 cttaaataga tcacatttat gaaaaagatt cataagttat aaaacaacac ctcaatgaaa
28261 aatgctgtat ctggacttta aaaagaagtg aattaaatac taattttacc tcttagtata
28321 tatgtgatct taatgaagtc cttagttttc ttgtctgtta aataataatg gtaagggcac
28381 cttctcatat ggtcattgtg aagcttacat gtataaaaag ctcagtacca tggctgacac
28441 atagaaagta tttaacagtg ttcaaaaaca taatcaatat aggaaataag tattatttta
28501 tttccttaat aatttcttag ttatacccca ttatcatatg tgataagaga atgtacttta
28561 tttctagttt tccagataat ctgtatccta aacattcttt actcccaaat tcagtctttg
28621 agcatcctaa ctcttgacat ccacagctaa tatttaggaa acattaaaat ttgtttgtac
28681 atttgaagga agaactaagt aaatggaact taccaatcct aaaatccaga ctgctgttat
28741 taagaagaag aacaactgta caaatgctgt taYcaaagga tgtatgagtc aggcggaaaa
28801 aaaaggagaa cacagaaagg tgaaaagtct ttgtcaagca gaggagagaa attattgtct
28861 tgtaatatat atgagagagt ctagccaaat attaaacatt tttaaaatga aaagaaatat
28921 tatacatggt atgaataata tttaagtcat tgcctaggtt agaagattag atatatttac
28981 ctattctacc tacttcaaat gcttttttgc tgtcattgtt tggaacctcc aaatactaga
29041 atgctttaga attataaaac cattgaccat gttcagttat gtttaaaaga aaacatcatt
29101 ctgaggaaac tggaaattat atatttataa atgtttgtcc atagcattgt tgaaacaaat
29161 ggctcaggtt tcactgttaa tccagttgct gatatttttg actactcaat acaccaaaat
29221 ggtacagtca agttggtgct aatagatgtc atttatttac acaaactgac aataggttca
29281 ccaaagttaa gagacgaatt taagggggac agaaaagaaa ttcctgtgtg tgctttggca
29341 gcttagacat acttaccacg aacaagatta tatcttaaaa gctattcgtt aagcttaaaa
29401 ttaagttata tggttgtaat atgtggagaW agcacattgg aatagctctt cacttaatgt
29461 tatagatcac tttttccacc aatattattt ggtctaggaa agtaaaatcc tcttgaggaa
29521 gtaattagaa aaagcacaca tcagggaatg taattgaaga tagacttttg ttgacctcag
29581 aagagaaaag ctcaaaagaa atttcgtaaa gttttaaata tggcaagagg accacaatgt
29641 ctttgataat tagactttca acacatttgg atttgctaat tggatctaag ttctaattag
29701 gctttttatg taactaagaa tgacatatca ttgtatgaat ttaatgtgct tttacagttg
29761 aaactaacaa ttagcagcct tttccaaatt attttaataa ggaaaaagct ggctctgctg
29821 tttatactgt gacattccct tctcttaaga tatattatgt acattttcat tttctWaatt
29881 aaacaccttt tggggagctt aaagggaaac ttgggtttta ataggaagca cctaaattgt
29941 ttatttgtgg gggagacttg ccagccagta tgtagatgac tttggaagct acagacaaac
30001 cgcaataaaa agaaataaat cacaatcatc aggaggaaac agcacaaata accatttgcc
30061 cctatgacca ttcagttaat caaaacagtc tctgaatatg atgaaagatc tgttacattt
30121 ttaagtgtgc acatgcggaa tatgttccca aatgtgaagg ctcatggatc tgctttcagg
30181 atgaataata ggaaatttct tctcaagatg tgcattacaa cattctctct ctctgtagcc
30241 ttgttctgta aacagttatc tgctaattca agtcaaatca tctctttctt tctccatatt
30301 tttgattgag agtcatcaat ttttatccca ttcagatata tagacagtaa tttaaaaact
30361 ggtRgccctc agatttgtac attccaagag ataagaccta tataacagca taaaattaaa
30421 ctactatttg ggtgagtaaa gagcagtgag tcattgcaag actttaaggg aaaggctgct
30481 gccaaaatat tccaagcaaa ttgataacac tgccaaaaag gcaaattata ttgttttatg
30541 tgctatgaat actttgaaaa caaagacata cacatgattt ttttcaaata aaagcagttg
30601 cccttctaac catattaaaa atagtgtctt ttttcccagt aaaagccata taagatcaaa
30661 ttaaaaccat tgaaactatt agagaatcta acttacagta attgatatat taaaaaagac
30721 attatgttag agttatatgt gctattattt ttccaattag tacattactg ggactgagtg
30781 attggtgtat acataagtcc tcattaagta aaatgtcctc aatgagcctc ctaaagcaca
30841 ttgggtactg tcatgtgcca tataatgata tttaagagca agaaatggca tatctaataa
30901 cttcatttcc tatttaaaaa gaaaacctgt gcagtactct ttccatacca attattctaa
30961 gagctatcac atagttctcc ataaagacta tacagaggtg atgggactgt tcttcaggca
31021 gaaaaatgac caaagttgtc ttatctttca gcttggKtgc tggaatcctg tcacaggtct
31081 gaatgggtca ctgactgaca agaaattgga gaataacatg cgtggagtgg ttctacgtgt
31141 agtaactgtt ctggtaagta ttatctgagc ctcgtggttt tgacttttgg aggttacttt
31201 atcctatatt tctttcttta ttaactgatg acataatctt aaaagagatt ttaacaaatt
31261 ctaaatacgc tttctaagat tttttaatgg tcatatttca cagccttcaa tttacgtgga
31321 tcaggatcac agttttgcag acttttaact acagtgtatg tgttaggaat tttcttagaa
31381 attttaggct caatttccat cctacagttt tctacacatg acaaagtaaa ggtatgtctc
31441 aggccttctt tcattaaaac tgtagtgaac atagatagtc aacatatagt gaacattgaa
31501 actataaatg aacataaccc tgaccatgcc aaacaatggt agatttggta aaatgtacat
31561 tctctaatag tgaaatatta acaaactaag aatctttttc tcgtaagaac ctacttcaaa
31621 gggaagctgt tgtttaatag gcatctactg tcgactgtat tccaggctca gtgctgatta
31681 ctgagaacat tgacaaataa aatgacagat ataattctgt cttgatagaa cttatcatct
31741 agtgattctg tgaatgttga ataggattac agtagtttcc cYttatctgt gggggatgca
31801 ttccaagacc cccagtggat gcctgaaact gtggatagtt ttgttttctt ccaacacaat
31861 cacattgtgt agggcttata ggttatagag tgtggatatg ctggacaaag ggatgagtca
31921 catccagggc atgtcagtgg gatggtgtga gatttcatca tgctacttag aacaatgtta
31981 aattcaaaac ttataaattg tttatttctg gaagttttca tttaatattt tttggaccat
32041 ggctgattga ggagtaaccg aaactggtga aagagcaacc atggataaga agagtctact
32101 gtatacctgg gaaagatctg ttacaatttc atttacttat taaaaagtca ataaatggta
32161 actgttataa ttctatacat tatagttttc tttcctaata gatacataca ataagatgtt
32221 aatagaaata ttcttaaaca gtaaaatact caaatgggct ctgtctaaat agccataaac
32281 cacgaaggca tacattctcc tttgctattc caatgtgttt aatcatgtgc tagtttatag
32341 cctgtttcaa aacgtaatag tccatagtta gtactttatt tttcttcaga aaagtctact
32401 tgtttttaat tttgaaaaag attttcctat gggctaggta aattgcagaa ttagtaaata
32461 ggagaggaag actttttgat acgttgatgc taattggatg ttcatcaaat tttataacac
32521 cagttgctgc cctttgtaca ttgtttacta caaattaaat atatgcaatg taattttatt
32581 gtcaaaaaag tggatttgtg catattttct atgaaaggac attcaagtga gtgacctatt
32641 cttctttttt tgtggcccat aaattattcc tagttattat ttttaaaagg gcttaattgc
32701 ttttggtaaa taattgtaag tatttcacaa cttaaatgtg aattcgcttc tgtatgtacc
32761 taagggtatt gatatgcatt tacattgatg cactaatatt aaaaaagaca gatatttaaa
32821 gtacagcaaa ttattaatag tctttcctcc agtgattagc atgaaaactc cagtgatgat
32881 tgcagtgaaa gacggtatat gtgctttgtt ttcctttgag aaaagaaagc aaatctttaa
32941 gtccaattaa cctcagtcag ttttaagcaa atagttattt cgcaattact atatgataga
33001 ctcagtaatg gatataagag aaataatata cagaccttgt cctcaaagta ttcatagaat
33061 cttgttaggt tgatgcacat agagaaaaac agctcagatt gattaaatat ggttgaccct
33121 tggtgtctgt gaggcattgg ttccaggaaa cccccacacc aaaatctgca aattcbtaag
33181 tttctgatat aaaatgatat agtatttgca tataacctat gtacctcctc tcatatgctt
33241 taaagcattt tagattactc ttaataccta atacagtgta aatgctatgt aagtagtggc
33301 tctaccgaat tatatattta tttttgttat ttttattggg tttttttcct aatatttttg
33361 agcctcagtt tgttgaatct atggagttga gtctagatgM agaacctgtg gatatggagt
33421 gttaactgta cttactatgt gcaagctact ctactaagtc ttttgcatgt attgtcttat
33481 tttgtcctca caatattctc aagaaatagg taatattctg atctcaagag gaaactgaga
33541 catccagaag ttgaaataca tacatgtatg tgtgtgtgta tatatatata tatatatctt
33601 atcattaagg gaaggggtta aggtctagaa tcagacttcc tgaatttgaa tcctaactct
33661 gctacttact atctgtataa tgttcataaa tacatgaacc tagaagaaat tgaagaataa
33721 catgcaagga gcgattctac gtgtggtaac tgctctggta agtattacct gagccttgtg
33781 gttttacaga cagtaagtag cagagttagg attcaaattc aggaagacag attctagacg
33841 ttaacccctt ccctcttaat gatacattat actgtctcca gtcctactgt gataagtgtg
33901 agaactgagg tgtctataga attttgtttg acttgagagt tgaaaatgct tacgtctgaa
33961 taaggtgaaa gtgagtttta ttgggaagga ttgtaggtac aatcatgtta aattcatttc
34021 caggctgaat aaaacaattg ctattttgtt catgaaatag tagcacttca cacagtgtta
34081 acacctgcat accccaggtt caccttcatg actcttaata atctctattt cctgcaagct
34141 tagccctgca cagatattga atatggtttt gtgactggag acatggaagc agtgtttttt
34201 tttttttttt tcaagtcttc tatgatggac ttatcagttg ggtgccagtt gagtaacagg
34261 ttgttatact tattacatta ctacaatata caatcattgt gtttaataat tttgcatgct
34321 tcctatgtct ttctgtaact actctgagaa tggaaaccag tcattccctt cttatttatt
34381 ttctacaagt gccttttcag gtctagctct tagttatatg gccttgcaag cttcttctac
34441 tcaacactga gaaccactct cccctcaccc cacccagcaa ctcccgaatc ttgaggcttt
34501 acctcactaa accctgaaac taggatttaa tctatactca acttgatgtt gtcacctaaa
34561 cgcaacctcc caccccctta tattccaagt atttacgtcc aacactttcc agaagagaaa
34621 agggaaagga ccaatgtgta catgggaaga agctatttcc tcctttcttc tattgaaact
34681 gaaatctagt cttagctcta cccactccct cttttcttaa gaaattttat attatcaatt
34741 acaccaggtt aataaaatgg atttttctta ccaaaatttt gaaattccca ttcttatgac
34801 tgttgatatt ttattgactt gaaagaaata acttcatatt tattttaaac tacacaattc
34861 agcactctac tgtttgtgat cccatgggca aataagtggt gctctcaaac atttcattga
34921 agagttaaat agaggtatta ttgacgaaga tgtaaatagt gttaaaggaa tcaacaagga
34981 gtgttgaatc accagaacct acctagatga cttacatgaa gactgataag gcatgggagg
35041 aagcattatt actagacaaa gcaatagagc catgggagag agaccatcac tcatagtagt
35101 agtgagctac agtgcagaaa aagaaagaga atcactgcca ataccaaaat caggctatga
35161 ggaactggga ataagggata cactgatctc actcctctta tctatctctt ttcatggtct
35221 ccaactgcaa atccaaccag aagtcagagg ttatgggagc ctaagtaagg aagaccatga
35281 aagttagtct ttcaggacac agaggaaggc agtgaagccc tcagtgaatc cagaagaaaa
35341 gtagagaaaa accaacacga gcatctacca tttgatagga cctattataa gaaggttata
35401 tatattaact tatttaatcc ttataaaatt tcatatgagt ggcactaatt acattataag
35461 gataaaaata gggtacataa gagttaacta aatttctgcc tccatatagc tagcaggagc
35521 cagacataaa tttcaagatc agagatcctg gttccacaaa ccatgtcctt agccactctg
35581 ataatctKcc ttctgaatca gaatggactc atgttaggta taaatgaaat ttgatataat
35641 tattgagatg tgaggattgc atatgttgga ttgataactt aggagaattt tcctaaattt
35701 cttcatatat agatttcttt gtattcaacc atcaccaaag ccacagaaat atgtcttaga
35761 aatggaggaa aagaaagaat gataataaaa gcagtctcta ctacttggtg tctcttctaa
35821 tctctttgtt tccataattt gcccactgat ctttacttct taccaagtgg tccaagtgta
35881 ttactacaaa ggaattatga agattttccc gtttttaact tcctgagtta tgcaatgtag
35941 atagcaataa atgaccaatt aagtcattca attgttttca tggtaattca tgaaaacact
36001 attggaaaca ttactgagtt ataaacataa tcataatata tatgataata aaatggaaag
36061 gaattggtta caaattaaaa gaaaggcgtg cgaagctatg ctgcaacaag tataagatat
36121 ctgagaacca taaattggca tttgggagga gctcactgat tagtaattct tatttaccta
36181 ctttgattgg ccttaaaaaa gacatataca ttcctaataa acatacattc actcatgaat
36241 tcacacaaaa cagtcaaaga tcctgacttt agagagtttt tttgttgttt gtttttattg
36301 agacaggctc tcactccttc acccaggctg gagtacagtg gcatgatcat ggctcactgg
36361 agcttcaaac tcctgggctc aagaaatcct cccacctcag cctcctgagt agctgggact
36421 acaggtgcat gccaccgtgc ctgactaatt tatttttcgt agagacaggg gctcgctatg
36481 ttgcccaagc tggtcttgaa ctcctggcct caagtaatcc tcccaaagta ttgggattac
36541 aggtatgagc caccgcaccc atccagggag tttgattctt gcaagatatc ttctaaagct
36601 ctcaaaatag tcttgaaaaa ctgcagtgca gttgttctcc attaaataca caatagagca
36661 tttttttgcc tagaattacc ataaaaataa ttcaatatat aaattgaatt catatataaa
36721 tatatgactg gaagtctata gtaaagactg tgatagtgat ttttctgcat tttataaatt
36781 aaaaaacata gtttttcata ttttaatctc tctaaaatta ggatgcatcc ttcaacttct
36841 atttgcaatt gtaattagca ccatgtttgt ctttattagt gatgtgtaaa atagttgcac
36901 atcttacaat aggaaacatt ttagattaaa taatgtatgg tatttttaca aattaagtta
36961 atggatattc ctggaaagtt aagttaaacg tcactgggca agaagccaca taaagagaat
37021 aactatttta aagaatacct ccaaaagaaa aggcagtagg aaatatacca attagaaaac
37081 ataggacagt aatagcaatg agaaaaatta ctgtctaaat taaaactgtt gcctcaatca
37141 tagaaggata tacacagaaa taggaaatac acagagatag cttatcttga atcattacca
37201 caaagcaata tccaaaatat ctattgagag aatggtaggt ttttctaagc aactcaccaa
37261 aggaaggatt gtttgcctgg ctgcagcaga cagccaccta ctgagttatc attttgagta
37321 atttcttttt cctaaaaaat atccatcatt agtctcactt tttggaattt cttttttttt
37381 ttttatggag attgtgactg atatatgggc accagagtct taatttgtac aaaaacaaaa
37441 aaaaagtatt taggttagat ttagttattt atatacatca gcatgaagta aagcaatgta
37501 cttaatttcc tcttaatata ccttttaaca aaggaataaa taatatataa ttcatttttt
37561 attcaaagta cttcacacaa ttttgttgcc tttatttcac caagtctcct ctatttaata
37621 cacctatgct aagaagcttt ggcacacact gcaaaaagca ctSaacttgc agtcaaaagg
37661 tatgactttt agtatcgtac tagtttttct gcttaattga tctttgaatt tatggaaacc
37741 aagacgctgt tctgaacttc aatttttcca tctgtaaaac aggaataata aaaccaatca
37801 caaagggtta ctactatatt aactggatga aactatgtga aagcaggtag caaaatgaac
37861 agtgYttcta catacattat ctctgcagta aaactatgat gtgtaaactc agtaaagaaa
37921 aaaaagcatg agtgatcata aaagtctaat tattcattgt catcaaactt cagatttttg
37981 tgggagattg gaggactcta agggagacaa acaaatgtaa actgcagaat aatgagtttt
38041 aggccccgtc actgttacct caatttgaaa acctgctaat gaggaccaca ggaactcttc
38101 tgccagtgaa gctggtactt cctagaggaa aaatgcacaa aaaacaccta ccaccgaaaa
38161 caaaagcacc ctgaattatg attttcaaaa atatctagaa gtttatatat caattatWtt
38221 atctcatata aactgtgttt gattccaggc tagctttaaa tagactctat attttaatat
38281 ataactaaac aaatgcctcc agtgatagtt ctgaaacgtc attgtcctta aattttttgc
38341 taaggacatt gtccaagtgt ttgctactta gagactttca gaattttcat cgctacctca
38401 aagttcaagc ttttctgagg tcccagcgtt tgagcctctt gaacctggag gcaaggttag
38461 gggttgggaa agtaaacttg tcaagagact ctactgggca agccctcaca gcaaggagtt
38521 gggaagagag ctgacagcaa gctctggtgg ctcccacagc aatgtcttac gagggctcct
38581 tcggaggagt taataaataa taaagtttgg ttcaggactc caggcaagaa tgacaactct
38641 ggctgaggaa atttccaggg gctgatatct aaaataaact taaagtcaca ataaaatatg
38701 ataagtgcca tgtaataatc ctacaatgga aatacacagg atagcattgt ggttaagaac
38761 acagttccca cagtttcact tctggggtgg caaatgagga gttccatgga cccactcccc
38821 agcaaaataa gcagagatgt caaaaatgaa aaagaaatga acaaaaaaca acactaacaa
38881 aaaagtttac attctctgga aataattgca aatcatatat ctcataagtg atatttagca
38941 tatacaaaaa tattataact cagtatgaag aaatataaac aaaattaaaa aaaattaaaa
39001 tgggcaaaga tctgaacagt tctgcaatga agatatattt atataaatgg tcagtaagca
39061 aatgaagaga tgctcagcat agttttccat caaggaaatg caaatcaaaa ccacaatgag
39121 ctatgactac aagcccacta ggatggctat attcaaaatg acagataata acaaatgttg
39181 gcaaggatgt tgaaaaatca gaatgctgat acattgctgg taggaatgta aaatggtacg
39241 actccttcag aaaacagtct agcagttcct caaatggtta aaagtagagt cactacatga
39301 ttacaaattc cactgtatat ataatcccaa gagaaatgaa aacatatgtc catatgaaaa
39361 cttgtactca aatgYtcata gcagcatggt tgattgcagc caaaaatgga aacaacccaa
39421 aggtctatca actggtgaat ggatacataa aatgtagtat accataaaat ggaatattat
39481 ttggcaatag aatgaaatta catgttgata acagtgtgca gcatggatga accttgaaag
39541 catgctaagg gaaagaagYc agacacagag gatcatgtat tatatgaatt catttctgtg
39601 aaatgttcag aatagacaaa tctataggga ctcacagggg caaaaagtaa ttcgtgattg
39661 cctaggtctg ggaaagtttt ggagattggc agatgacagc taacaagagt ggcatttttg
39721 agggggagct ggtaataaag ttctaaaact gactgcaatg atgattgcac atatttgcga
39781 atatactaaa gctattggat tgtgcacttt ggatgggtga attgtatatt atRtaaatta
39841 tatctgaata aagctacttt ttaaaaaaga gagagtctgg ttccccagac tgcctatgtt
39901 taaatcccca cctctcctac ctgtgaatgt aggccaggta tttaaactct atgattctgt
39961 ttcttcatct gtacaatgga gataatataa attacctacc tcataaagct gttataaaaa
40021 ttcaaataac tagtacatgc aaagaacaaa gaccttctaa cagaacctgg cacatgaaaa
40081 gtgtgatatg tgtccattct tcttatgcac agactgttaa gggagagcat gaaaRaagta
40141 tccaacttat gggtaaggca gtcggcaagg cttaaaggat gatatttaaa ttcatcttga
40201 aagatgagca gggattatca aggaatagat aagagggaag ggatatccag cattagaaag
40261 agtatatgcg gaagtatgta gatatgatat tctatcaaga atttaaagta tcctaaaagt
40321 aaattcaaat attttctgaa gtatatgaaa ttgaaataca gaaaattcca gcaacctatg
40381 tgtcatgtct tctctttttt aagagttcct ttagagacaa ttcagtaaga aatttctctg
40441 tgactatgaa ggtaatattg tatgatgtaa ataatattca ccaagttaaa aataacattg
40501 ctacaactct gatccagtgt aaagtttagg taaaatagta ctcattttta ttattgaaac
40561 agtgtaatca gtagacatag ctctgtgttg tgtacatatt ttgttttgtc tcttcttctg
40621 agtgtactca tcttaggtga atggggaaaa tatgattttt ataaacagac tcttaaagaa
40681 aaatggccta tgcaaatgat gtaatttaga gcaaccatag tatatgtgtt cacttcagcg
40741 ctcactaaat aaaatcacca taagaattga tgcatgacaa tggcttgaca atttcttatt
40801 cttttgtatc acaccaattc ttgaaatgat agctgttgca cagacatgtg tgagtattgc
40861 tattgtatat gtggtagcca tgcatgaccc tacttttctc ctcttggaag ctggaacctg
40921 aattcttaga ctaatcaatg agtaaatatt tcagagatgt gttatctaat ttacttctga
40981 aaaacagatc tactacttgt ctctagacat ttagaaagag attgacagag tcatttgcaa
41041 attcaaagat ataaaaacag taggtagatt gacttccata atcaaatcaa tatttggtta
41101 gaacatttca caatattcta accgaaatta catgaactac attatatact ttcagaaatg
41161 cagtgtacca ctccatctga acaaaaacat aatgttaagg tgtccttgaa gctttaagtc
41221 atcttttggc aatgcagtag tttcaacata atcacaagac aaaaccagtt ccacataaaa
41281 ttcttcagaa atgaacatga atataaataa aaacttgaga gatgctactg aaatagattt
41341 tacatttcag agttacaaag gaacacatta gtagctatta aagcttacag ataacattac
41401 catctagctt actaataaat ctcattgtta gtattttctc tagtctttac tgaaccttta
41461 agattattaa tttttttcat attttattga tatggctagg atagagttat taaataactt
41521 cttcaagtta caaatatgct aaaaataagc attgaatcct tatctatcta atttactccc
41581 agccatgatg attctaacaa ggactggcct aatttcctgc cacaaaaggc tataaaactg
41641 agccaagtat ggaaaaacct tttcagacat tagtcaattg gtagaaatgg actttgaYac
41701 ctaaaaggtt ggaaacaact aaatgatccc aacaattatc ccagctttct tgctgctggc
41761 agtttccata ctggagacat gaaagtatgg atccaaaaca gaacttgtca gcctcactga
41821 gtttacgaag tagagatcag agggtgagaa agctgatgtg gttggcgcat attatctcat
41881 ttttgagata tgttgtaaat tagagggagc tgagcaggaa aatatttaca gaaatgtgaa
41941 tcttgcctga gtactagacc atcaatgcta gagagaaatt ctacaagtgt gggcaaagaa
42001 tgacaggaat cagaacaatc gccagagctc acacatgatt tttgagaaag gtttcatttg
42061 gggagatcgt attgtatcaa gaattcaaaa gaaaatgtag aaagtatgta tttatctttg
42121 agttgtgtag tgatcgatag aaatttaggt gaatatctaa atatctatat agatataaag
42181 taggatactc ccacacacct tgctatttct gtgttttaca tatttccctg acctcccaaa
42241 atgtccagYt ttttgttagg aagctgcacg ctcatcttcc ccttctgagc tccaagagca
42301 tgttgatgtt cttgctgttt catagctgat ataaattagc attgggtaaa cgacgaaaaa
42361 acgtcacaga aagtttttct tgttcacatt ttggaaatct tccttctcag tagagaaaag
42421 caaccttaaa gttgcaacat catgattgga tttcagttca caaaagaaaa taaattttga
42481 caaatcacat atgcttttcc tgtccctccc aaaatgctgc agtcttccat ggcagcctta
42541 ccaaagaaaa aactaagtcc ctaactggaa Sttggattct ttcaaaggca cagtaagagg
42601 ttttgaccaa tagagaagca ttgatcaaat cactatccac tgcattacat ggtactctag
42661 gagagtctga aatgagaata gatatcttca aaaagcatcc aaatctgggg ctggtttact
42721 catctgtcag cttctggaat agtgaacatg ctattaaaat tcagctcctt ctgggaggtg
42781 ggaagataat caaggggaga cccaccatac tgcatggaag tcccagaaat ggaaaagctg
42841 atcaaaggaa ggaaggtaat atactttttt ataatatgtt ccttgggatt tttaatgatc
42901 ggtcgaaata cagaggtttt ggttttaagg tgttattttc acagcatctc caggtaaact
42961 tccaagtatt atgttaRcct ggatagaaac aaaaaagtcc ataatgcttg aaactgaata
43021 tgtgatcctt gcatcatgtt taaaattgtt cagcaaggaa tttcagtttc tctaggtgat
43081 ggaagttagc acgagcaaag gaactttaca atttttcaga attttttttt tttcatgttt
43141 ggaaggtctc ctcctcctcc tcctccttct cctcctcctc ctcctcctcc tcctcctcct
43201 cgtcctcctc ctcctcctcc tcatcctcct cgtcctcctc ctcgtcctcc tcctcttcct
43261 cctcctcctt ctcctcctcc tcctcctcct ccacctcctg cttctccttc tcctccttct
43321 tcttcatttt tatttttaac cattgctagt ggtatgattt ttgaattggg aaaacaaaat
43381 attcagacta aatattttcc caatgattct tatgtagagt aatgtacttt gcaattcaat
43441 taaaaataac aagctaggat ggtgttctga gccaaatgta atctccaaga ccatggtcag
43501 taaagtactc aagaaaaaag tattgaggcc gagtagtctg aagtctaYtg gggaataaag
43561 aagcagctga ttagcatttg aaagcaaggt ctttcactgt tttaaaagtg aaggagaatt
43621 ttttaacaga Rcactcatca aaaacaaagc gtttgaaaat tacccctcct ttgttttatc
43681 agtctcaggg aagatgcact tcgaYacacg tctcaggaat taagatgcat ttcgatacat
43741 gtaatattac atttggtgac actgctggaa catatgctga gaaatgcaag aataaatagg
43801 actaatgctc attttcNttt tttttttaat cacttgcaag tgacatttgt ttcatctagg
43861 caggaaagca ggatcttata ggcagggtag gtgtaagggc tgattttagg tcaagctttt
43921 caatttatta tgcaataaaa tatagcaata aaaatatgca tctttatatt ttccagtaaa
43981 tbataggaat ctccgtttaa ttttttatat atgtgctaat aattaattca gtactacagt
44041 ctggatccac agtccttttg ataagagctg gcacagaggt catttttatg tgccttatta
44101 ttaatcttaa taatatgtag taatattcct ttatatgctt attgtgacac acagaaactt
44161 aaagcctttg tagctttaag ttcagatgtc tccacattct tttgtaaatc cctgcagatt
44221 tgtttcttgc ttttaaatat cagcaccttt ctgctgtcta ctcttaggtc tctgccctct
44281 atgcaggaga tcttttcttt taagtttccc accgtgcttc ataataaaat caaaagaaaa
44341 aggtacatcc ctcagaaagc attcatctca ctaKaaatag aaaacctgtc accttattct
44401 taaaacctta gatcagccag gccagtggct gaaatagtga gggtagtgtc tacaaagggt
44461 ataKagtagg ggtagcaaaa taattatgta ccttgttctc tccacatctt ttagggaaga
44521 gactctttgc tctggaaaat gacttaatac cattgggagg aaaacatttc ctctacccac
44581 ttaggttcct atgtttaggg gcctgtgaat taactgataa tagacagatt aacaggagaa
44641 aagaaaaggt taatctacac atgcatgtga agtcactcaa taatgagtcg tttcctgaat
44701 acccagaggt aaagattggt atagcaactt aaaaaaggga agggaagtgt ggctagggca
44761 tcaatgtgta gttgatctcc ttcctggRat tagcagaaag ctcttggagg agagttaata
44821 gcagctgtat tgggggtagt gtcagcttta gttaggtaag ggaaagtcag atcagatttc
44881 aaatattttc actttaatct ttatgctaaa actgtgattc cttcaatatg ctgatgaaag
44941 ttgaagtgtg gcttggaaaa aaagagaaag tataagtaga tttattctag atataaagca
45001 ttttttaggt atagtagaaa gagagaaaga gaaagcaaca ggcacacaca cactgagaga
45061 gagattttga cacgaccatg tcaaagacta agagtacccc aagttcttta tcaaccatgt
45121 agtttctgca acctgcagta tacacacatg catacccata tactgatgga ggcagtctaa
45181 attaatggtt aagaatatga acttcagcac cagagggcat tggattcaaa tttcaatctt
45241 tcttactagc tttgtaactt caaacttttt tcctcttgga agctggaaac tgcattttta
45301 gattaaacaa tgagtaaata tttcagagat gtgttataca gtttacttct gaaaacagat
45361 ttattacttg tctctagaag tttagagaga gatcgacaga gtcatttgca aattcaaaga
45421 tataaaaaac aggtagactg actaccataa tcaaatcaat attggtttag aaaatttcac
45481 aatattctaa ctcaagttat gtgaactata ttatatactt ttacattatt tatctaccct
45541 caagtttcag ttctctcctt tgcaaatgac aataacaata ccaaaaaata aaaaaactaa
45601 atgaggtaat gaacgtaaag tgataaggat gatgacaggc ataaagtaag ctttttattg
45661 tttttatttg ctttttcctg tagcctgaaa atatgttcaa ggtaaaaaaa agcattcttt
45721 tgctctgaat ctcctttatt ctaccaattc ttttctcgtg taagtgtctc agactcttca
45781 gttgtcttca ctttgtcatt tatttttatc ctcaaatcat tgatatttgg cttctatttt
45841 cacttctcca aacaaattgc ttttttgtct tcatgatctt ctacgtgaca aattaaatgc
45901 atgctttcag accttacctt cctggacctc tcacctccat tggaatccac tggcaattta
45961 ctcccagaag ccctgtcctc tcttgcatct tcaacatctt ctgattctga cctctctatt
46021 cctttgttta tttgtttgtt tgtttgagac agagtttcac tcttgttgcc ccaaatggag
46081 agcaatggca tgatctaggc tcactgcaac ctccatctcc tgggttcaag tgattctcct
46141 gcctcagtct cccaagtagc tgggattaca ggcatgcgcc aacatgcctg gctaattttg
46201 tatttttagt agagacaggg ttttgccatg ttggtcaggc tgatctcgaa ctcctgacct
46261 caggtgatcc tcccgccttg gcctcccaaa gtgctgggat aacaggcgtg agccacagtg
46321 cccggcctct ctattccttt tacctccttc attggattct cttcttccag agtttcatcc
46381 ctggccttca ctatacttta ttgaatgact tcatttactt tctagaaagt tataaacaaa
46441 tttttatttc caaccctagc ctctctctcc tgcttcagac tcattatcca cgagtactat
46501 tgaaaaaagt attctatatt taatgacaaa aaaacagaaa atatggaaaa caaaaattaa
46561 cagcaaaatc ataaataatt cttccatcaa aagttacttg ctaatatttt agtgtctacc
46621 actagctaaa cttaactaca tttccttatc tataaaattt gaggtaattg aggtcccaaa
46681 ctcataaaac tattgagcat gaatacacaa aaagttttta aagtacccag cacatactaa
46741 gttccatatg aatatttgct gctattattt ttttcaattt caaacatgta cacacatgtt
46801 tcaaattcaa aaatgtagct ttgtacattt tcttcccatt tagcaatatg caaatgagta
46861 tttttaattg aatagatttt tttttttttg agacggagtc ttactctatc actctgtcac
46921 ataggctgga gtgcaatgcc gtggtcctag ctcactgcaa cctccgcctc ccgggttcaa
46981 gcgattctcc cgcctcagcc tccagagtag ctgggactac aggtgcatgc cactacacct
47041 ggctaatttt tgtagtttta gtagagacga gtttcactat gttgtccagg ctggtcttga
47101 actcctgacc tcgtgatctg cctgcctcgg cctcccaaag tgctgggatt acaggcatga
47161 gccaccgtga ccagctgtga atagatattt ttaacagatg tgatcagtag attttttcag
47221 ccacttagtg aataatttct acagctgcct caaatgtcta ttaatttatc tagggttgta
47281 tgtaattccc agccttctaa ctgttgacag ttttatttct cattccctga actatgttga
47341 aatgcaaatt aatgtcttta cagagatatt tttgaatctc tctattaaaa aaaaaaaaag
47401 aaaaaagaaa aaaacaaacc aatcccgaac ttcaaaatgc tattcttggt tttttaaaaa
47461 atgacctata gtccaactct gtgtagataa ttatgtgaca gggcattgag acactgaagt
47521 tgacagccca atgtgctgtg ctgtgtcata cagatagact taaaaaaaaa aagccccagg
47581 ctgggttctg tggctcatgc ctataactcc agcatttggg gatcacttga ggtcaagact
47641 ttgagacaag cctgggcagc atagtaagac ccatcccccc cccccaaaaa aaaataccag
47701 gcataacagt gtgtgcctgt attcccagct actcaagagt ttgaagcagg attgcttgag
47761 cccaggaggt tgaggatgta atgagccgtg attgtgccat tgcacttcaa cctgggcaag
47821 ataacaagac cccatatctt aaagcaaaac aaaacccatc tctgattgta gcgtatttag
47881 gttgttttca acttcttcct agataacttg caatgaacgt tttaaaaata tatacttgtg
47941 cacatttatg ctcatttatg attatttaga tgtacaatta ttagatgaga taatataaaa
48001 aatttacagc ctttcacttt tatttttctt tttcttttat tttactttaa gttccaggat
48061 acatgtgtag aatgcgcagg tttgttacat aggtatacat gtgccatgtt agtttgctgc
48121 acctaccaac ccgtcatcta gggtttaagc accacatgca ttaggtattt gtcttaatgc
48181 tctccctccc cttgcctacc ccactaacag gccccagtgt gtgatgttcc cctccctgtg
48241 tccatgtgtt cccattgttc aactcccact tatgagtgag aacatgaagt gtttggttgt
48301 ctgttcattt gttagtttgc tgagaatgat ggcttccagc ttcatccatg tccctgcaaa
48361 ggacatgatc tcattctttg ctatggctgc acagtattcc acgatgtgta agtgccacat
48421 tttctttatt cagtatatca ttgatgggca tttgggttgg ttccaagtct ttgctattgt
48481 aaatagtgct gcagtaaaca tctgtgtgta tgtgtcttta tagtagaatg atttataatc
48541 ctatgggtaa atacccagta atgagattgc tgggtcaaat ggtatttctg gttctagatc
48601 cttgaggaat cgccacactg ccttccacaa tggttgaact aatttacact cccaccaaca
48661 gtgtaaaagc gttcctattt cttcacagcc ttgccagcat ctgctgtttc ccagcttttt
48721 agtaatcacc attctgactg gtgtgagata atatctcatt gtggttttga ttcgcattgc
48781 tctaatgatc agtgaatgtt gagcttcttt tcatatgttt attggtcaca tatatgtttt
48841 ctttatagaa gtgtctgttc atatcctttg cccacttttt tatggggttg aggctttcac
48901 ttttttattg tgaatttgtt ttctggaaaa cttttaccaa ttttaattca cataagtagt
48961 gYatgagaaa gtctactttc tcacatgctt gcKgataaat gttaacattc atttttatcc
49021 ttgccagtca gataagcaat aaattaaata gttcttgtta ttttaatttg tattccccaa
49081 aggtatgtta agagagcaaa acaataaatR attcaccctc aatggttgct gttaaacttt
49141 gtctttttaa aatttaagct ttaaagatta tgactttaat tattttttct tctcataaat
49201 aatatttctc ttctaaataa ttctaagtca caattatgta gggataaaaa agaaagtata
49261 cattttaatg ctttcctcca ttaaatttga atgtgtgtta attttgtatt cagtgctgca
49321 ggtttttaca gaatactgtc tttggttcca tattgaacat tcaccttgag gactttagtc
49381 tattgattga aagctttagt tcatctaata gaattattat gacacacact ccaRctaaaa
49441 atcagtcaag gacagtccca gaagttaata ctcttccaaa gactgatgcc tccactgagc
49501 acctctagga agtatgagat ggKgttgcat cacttctcac taagttctta taaccccaca
49561 tccaatatat caacaaattt catcagctct accttcaaaa attacctaga atccaattat
49621 gtcttattaa atccatttta ctgccaagtc acttctgatt tattgcaaaa ggctcctaat
49681 tgatctccca gcttcttcac ttgcctcttc cagtctattg tcaatagtaa ctgtcaactg
49741 tStagtaaac tgtgaaaata tatctgatca tgtctctcct ctgctcagag cccatctggc
49801 ttcctatctt agtcaaagta acagctgaca tcatcatagt ggcctaaaga ctctagtgaa
49861 gctctgcccc atccaaatac attgcctctc agatatttac taatatStgg ctctagcttc
49921 cttggtccta gccacgctag tgtctcttct gtctcttaaa caagtcagag actcttaact
49981 gctgtattta tacttactct ttcttctgtc tgaatgctgt ttctacaSag gtgctttcta
50041 attgaaggta caatcatttc tcacatatgc cttcttctca ttatttgtKt aatttctctt
50101 cacagcacct atcatcacca tctgacattt tatatatata tatatatata tttccttttt
50161 tgtttgttgt ctgtcactcc cactaaaatg taggtctaga agaatagaaa tgttcgtttg
50221 ttgtttgttt tgttcactgc tgtattctta gtgtgtgcct ggcattaaca ctcttttgaa
50281 tgactgaata aatatctgaa tttctgctat ttccctataa gtaatattat tttggataga
50341 aatgccacta atcctgaaac attttcaagt tttatatggt ttttatcttt aatatttgtg
50401 tggctttctg ctacattcca atatttaatt agctttagtt tacatttatt aaatgcttct
50461 atatccagat aatgtcatat cataagtttc agttgacatt tattaacatt actacttctt
50521 ataatatttt aacagaaata tgattcatca actccagtgt ttgtaaatat Oatcaccatt
50581 ctgaagtcaa atgttgccaa agtcttccat taaatacttg cagacttctc tcatttggta
50641 cagccagtca ctccttacag ctgcaatatg ttatatctcg aatgtatttc ttgatagctt
50701 tctttaagat aactgcactt gcaaagttct ttgtacctag aaaactttca acaaatattt
50761 agagaatata atttactcat gcatgtggat agacacatgt aagattcact taagtgtcaa
50821 atttactaat ataatgaaaa cttggaccaa acaagcaaca aaaaatatta tccagcactc
50881 atactcaaaa cgcagtctga gagtttggta tttttcagtt cctaaaggtt gatcatgtgg
50941 ttcataaaat cttagtctcc aaaatgtatt ttcaaatgaa tgtcaggcag aaccaacaaa
51001 tgagaactag ggtacttagg attattgtaa ttgcctaatg tgtttggtga ccttgtattc
51061 ccacttacag caacttatgc ttctttcaaa ttcttttgta caaaaaaaat tgtcttgtcc
51121 actctccact tagcctttaa gtcactaaga tgtggctatt tcattagttg aaagcaatgt
51181 ctaaaataaa atattttatt ttaaaaatca atcatcttgc ataataatag ctttaacttt
51241 atgctcaatt taaaatattt gtatttaaat aattgcttac agaatagaaa tagaaatgat
51301 atataagata atatagaaga tgtaaggatt taagaagtta ggatgacagt atttttattt
51361 acttttatca tttgtaattg tatcttattg tctggtaaaa acatttaagg aaagattaaa
51421 atctaagttt aatgtattat ttataaacat ttgctccttc ataaaatctt cctgcaatcc
51481 ctctttttaa aaatgttttc attgcatatc ccgatgatct gcttttcttc tctccatgaa
51541 actcagatac tagttttctt tacaacacct ggattgtttc ttcctctMct gctgttcctt
51601 attttgtttt tctattctgg tgtcttgata tctcctttta acaaattcca taccattggc
51661 cttcctaact tgtgaacatt tctaatactc acatatgctc tataaaaaaa agtgaggttt
51721 ttccaagaag aacagtggcc attaattctg catatatcaa aatggaatgt gtcatctttg
51781 agaaaaatat aagctcacac catgcataaa gacaactatt gtttgcttca gccaggagta
51841 tgttttaggg aaaaaatatt ttaaacaagc cattaagaaa tagtctcttt tttctttaat
51901 gtctttgagc tacatatgtg agcagaatta taaagaattt ccagtgactc attcttagtt
51961 ttggccttcc tggcattctg tagtcgtgca tattaaaacc tctaaacagc tctcagtaaa
52021 gccttggcag tagagcttgt agcttgtacc tatcaaaaat gtcagatgct tttttccaac
52081 gccatcacta gcaaacaata ttacttagtt attaacatat aggaattaac ggcaaaatct
52141 gcaagttgca aacagtgttg taaatcaact aagcatttat catgagattt tttaagtgac
52201 tgaatacaga ttcagaatca tgaagttatt atctaataac ccaacaagtg atgtgaataa
52261 atccttgcta atatgaaaat tatcagccac tgaaacaccc ttttctacaa gcaggtctgt
52321 ggatcctcat gctaatgtac ttagattgat aaaccacaga cattctctga aagcttcgtt
52381 tgttccagtt gctgaggctt gtgcttagag catctgtcat gcaaatgatc agcaaatcat
52441 aacctgtaat ttgttttcat ttaataggca gacaggtttt caccaattta gactcaagtc
52501 ctattaatac tctttgaaac taattgctgg aaaatgaatt ttaatgacag gattttatca
52561 tttattattt tcccttctgt ccctataaag gaagactcaa agccatctac tcacactttg
52621 aaaatatatg ttaaataggc acaagccata atctcctgtc atctgggctg ttgcaaagga
52681 cccctaatcc cctctcttgt aataatccat tctacactct actcttaaaa ttactttctt
52741 actatcccat ttcccttcca caaaatcttc aattccctgt caagtaaata gcagacactt
52801 taatatggct tcgttgccct ccattatctg tttgcagttt acatattttt atccttattt
52861 gatatcgcaa tataacatgt atacagaaaa aagcatgaMa cattaattaa taagtagtaa
52921 agtaacatcc agaaaacact actcagagca aaaagcagac gattgtcatt accacagaaa
52981 ccacctcccc agtgcatctt tctgatcaga cccttcccag catcccagag tctaaccacc
53041 cagagataat cacttcctct attttcttta gattgtctcc agctacgtgt gaattcttaa
53101 ataatatggt caatttaccc atttttgaat gttatgtaaa tggaatcaaa atctgctttc
53161 tttggctatt gcttttttca ctcaaattat tctcttaaac attcattcat tttagggtac
53221 agcttcagtt aacttcaatt ttaagctgtt tagtattcca ctgcatcaat gtaccacaat
53281 atatttatct tttccatgtt gatgggcact tggattttta gattgtttcc acatttgagc
53341 tattacagac aagggtgcta tgagcattct tggatgtata gtttggtgcc cagtattctt
53401 ttgtattcat acctaaggag tggaatttct aggacttagc catgcatatt catactagat
53461 aaatattgat gtaatacgta aaaccaagta atattaactt gtatataagt agctctgtat
53521 gctactagac aagtcaaaat atttttcaaa atggttgtaa atttatatat cgagcaggag
53581 tgaatgctat tcactcctac ttctatttcg acttctagaa ataggagcac ttctatttct
53641 ttatatgctt gacagtactt gggattatca gactacctca ctttctgttc ctcctctatt
53701 tattcttgct ttaggatttt ctatcctctt gtttattctt ccttcaccct ggatattaat
53761 tctgacctat tgcaattcat gaattctttc ttcatctgtt tctaatctat taatgactca
53821 gtcatatgtt aactttggta attgtctttt ctaattctca aatttgttta gttctttttc
53881 aactgcgtaa caacactcct ttgtagtttc cagtttcctg ccaattaaaa aagcattgcc
53941 cttcatttct tcaaacacaa taaacataat atttccaata tgcatctgaa aattctagta
54001 tctaaattct tgaggatcaa tttctgttcc ttgtttcttc tgcaggttct attattgaag
54061 aattattatg ttggtgcaaa agtaactKag gtttctacca ttacttttaa tggcaaacac
54121 cacaattact tttacactaa tctaatattt cttttcacca catttctttg atttttgttt
54181 ggacatcata ttcaaaaatt atttgaagga atcatgttac acctaaagta atattacctt
54241 tttctagaga ggattgtgtt tttctctgtc aggaatctgg agcctccatc agggtaccac
54301 cataaattgt gttaaaattt gagattaaac tatgcagtcc tggattttaa tccataggtg
54361 gaaaaaaata gagtttcaaa gaggtaagct tcaattaaga cagattatga tgcctccctc
54421 ttctgtaaca tattgtgtga ctttgagttg agattcttcc taagttttct gatctattaa
54481 aaaagatata gctggtaatc cccaaaatca tgattttcct tttttttttt tttttttttt
54541 tacagtttct actgtatttc tttgttgtcc tgtcagtacc agttatggtg tgtgttgctt
54601 tttctttctt tatatttttg ttgagcacag tgattgagta catgccccac taatagggta
54661 taattatttt gagagtaata tctatgactt aacaccatat ctctcactat attgagtgta
54721 gtaccttgat gcagtagcta gctgctcagt gaatgttaaa tggataaatc aatgactgaa
54781 taaattttag agataggtac atagataaaa gtgatcaaaa caagctaagt attactgcag
54841 agaaggaaca agtatctttt ctattatagt tcccaaagct tcaaccaaat ttcttttgct
54901 atgtttacct gtccaatata ttggctggtt gtactcatag agtactagtc ttagttagaa
54961 tttatgagta atggtccaaa tccgtaatag agcttaaaac taaagcttta aaaacattga
55021 tccagatgaa gaatgatagg atgcaatgaa agtttattta gggcataaaa tttaaaaggt
55081 atgttatcaa taattcattt tgaaagtccc cttaatatag ccatttctat tctgtatctt
55141 taaccagtta taggtgacat tgctaggaac agattttgac agcagacttg tgcaagggat
55201 ctgtttgcct agagatttac ttaaaaggta gaattaaagt catttcaaga aagaaattta
55261 acataagaaa atataaccat ttagtacact aaataattgg tctagctagt tatgagttaa
55321 aaatgaaaaa gaattaatag tttttagcaa catatacatt gggacagtat ttttgccaca
55381 tctatcattt acctatgcaa taattaaatt cataattttt taagtgtcat atcgaagagt
55441 gttattcaac ttcctggtga ttaaattact gataaacata gccgattttt tttggttttg
55501 caggtcctga aaaatagaga atgcatatga cttccaaata ttggtagaag agccagtcaa
55561 ataatatctt ccatgaatcc tcttacaatt aatttgctga ttgatagatg ttcaaaatct
55621 agaaatgaat gagttgcatg taaggaaatc agtacatgct tctgctgcca tcatatatca
55681 caatacaagc attaaaagct atattaatta tagaacttta tgatcttatt tacatggtct
55741 gtgaatttga tctttatttt ggaattcctt taatccattc tgattctaat cacctcagga
55801 ccaactttag tgtatttgta caaggcgtaa tcattggcta gctctataag ttgtccaaac
55861 aaccaaatga gatgtcgcaa attctgccaa ttgtgtaaag ttttattatg tactttaaat
55921 atatgaatat ttggaatatc aagatgtgtt gtattttacc aataatatta aaggtttgct
55981 tgttcacttt tcacttgtat tatgatgtca tttaaacaag tcagtattat tagaaatctg
56041 attcattctg aaaacctctc atctactgat ctaatgatct tcaatattca ttaacatgaa
56101 atattactat aataggaaaa aaatcggtac cttaacaaaa aatatggaaa tttatttgtt
56161 tatttattat ttttacttat ttatttattt atttatttat ttatttattt atgtttttga
56221 gacagtctca ctctgttgcc caggctggag tgcaatggtg tgatcttggc tcactgcaag
56281 ctctgcctcc caggttcacg ccattctccg gctgcagcct cccaagtagc tgggactaca
56341 ggcgcctgcc accacaccca gctacttttt tgtattttta gtagagacgg ggtttcacca
56401 tgttagccag gatggtctca atctcctgac ctcgtgatcc gcccacttcg gtctcccaaa
56461 gtgctgggat atttctttct ttctttctta cattctagag ccttggcgag ttggcccaag
56521 tgttgtacag cattttcRgt gtcagggaca tagacttctc tcctttctct gctgtgtgta
56581 gtctcagata gttaccagag caccagccat tgtatgttct ttctagtttt caggtagcag
56641 aaagaataag aaatgttgca caaaccaccc tgtttacatc acgttggcca gaattagtca
56701 catggccatg aagcaagcca ctttgcctga agtaggacta cagattgtct ttattctcca
56761 tagtggtaaa atataaaaaa atataattag agaagaagag aactcctctg gaggacaact
56821 agcagtaacc tactctacta actgtccatt tactagttca ttttgctcta agataacata
56881 taaacagtga aactaaaata tgttttcaaa attagaaaat gtaagtacct tagataacca
56941 agattaaaga aatgttacaa ataccacaat atcaacatag ttatagatga acatcaaact
57001 tagtactgat tttctgaata tccaaaatga aaagaaatac ctcaataatt ggttacattg
57061 ttttttatca ttttgaaaaa ggcactactg aggcaaatga agctctttga ccctgaacaa
57121 ttacaaaatt tatctcatag caatttttag ggagaaaatt gtatattata gttgacaata
57181 ttcctataac aggtgcagtg attttataag ctacttacag tgtctttgaa taaaagccaa
57241 aatgaaagca ttatgaaggc tattttgtga atctctgagg aaaaatcatt caaaaaaaaa
57301 aaaaaacctt tggctattct ggcttatata caaggagaaa ttttagcctg cccagagggc
57361 tggatggact acatacacca tgaatggtta tctgtaaata ttactgttgc tgaaagattt
57421 ctgaacaaat gtagaaaatt aaggaataga ttatctagtt ttctgtagtc tgccagcatg
57481 tttcaaatca atcgagcttt cttaacacca ggtgttctca gaagtgaaag gggcctattt
57541 gttagcttta aaaagacaaa aatgggaaaa gataaaagag gtggaagcag aaaccaattt
57601 gctaaattta ctaaattaga cagtttattg aaaaggtaag tgaatttctc atatcagtca
57661 taaagaagct atttttccaa tgaaatgtgt gtttatatca aagtcacaaa attacttagt
57721 gaataactcc cagtaaatgc gatattatta atttcagcta aattatatgt tcttagaatt
57781 tttttatctt ttcagattgt tttatgtaga ttatagctgt taaaatttta ttggatgtaa
57841 tttacatggt ttacaaacaa taaattcctg agaatagata cgataaattt atagtttgtt
57901 gaatgattta ttattaagga gtctcagatt ggtcctccga ggacaggaaa aataaattca
57961 tggaccctaa gttttttagt tacttttatc taatttttga tatggtaata attaaaaggg
58021 agatgataag aaattgaatg attaagcatt gttaaataat atgatgcgtt aataatgcat
58081 aagttatatt cctgagttga ttctttcaaa gtataaaaca atttttagat ttaatcaaaa
58141 ctctttaaag aattaatctt tgcttttagg gagcactata gattgacatc agaaatttct
58201 cttatttcca tgtaggaaga accttttgtg atggtctctg aaaatgtctt gggtaagccg
58261 aagaaatacc agggcttctc cattgatgtt ttggatgcct tatctaacta cctgggtttt
58321 aactacgaaa tttacgtagc accggatcac aaatacggaa gcccacaaga agatgggaca
58381 tggaatggct tggtaggaga acttgtcttt aaggtaagaa ttactttatt tatttgtctc
58441 atacttaaag gttcaattat ttgtctcata cctaaaggtt caacagtaac accttgaagc
58501 tgatttcata taaaataagt gtgatgtatt taggttggtt aagcattagg tggtggaatt
58561 gagtacaaaa gtctcactca atactatttt atgaactttg tggaaagaaa agtagttgac
58621 aaaataaacc taaatgttaa aatatatcct gtaatagaaa agggtttaga agaagacctt
58681 ttctttgctc aacttatacc ccaaaaacag aaagtgtata tgatacgtga tgaaatcaca
58801 tttttttttt gagacagagt ctcactctgt cctccaggct ggagtgcagt ggctggatct
58861 cggctcactg caagctccgc ctcctaggtt cgcgccattc tcctgcctca gcctcccgag
58921 tagctggact acaggcgccc gcccccacgc ccagctaatt tttttgtatt tttagtagag
58981 acggggtttc accatgttag ccaggatgga ctattgtttt ctttttaaaa atgtgatgtt
59041 attggaaaaa attaaaacat tcactgctta Raaactaatc atttcattct gtaatctaat
59101 caagataggK atgtacatat gtatatattt ttctttgttc tttcaattct cagaaaggtg
59161 tttaaaacct ctatgtcYtt ctttagtcct gtcagaatct gcttcagttt tttgaggctc
59221 tgttattagg cacatagaca tttataatta ttacattttc ctgataaatt attgaatagt
59281 tgggtttagg tttataactt actgtttggt ttatatgtat accatttttt tctctgttgc
59341 ttcttttcct gtttttcttg gggttaatta aatatatttc agtatttgat tttattgctc
59401 ttactgcctt tttagttgtc tccctttgta tttttgttcg tggttagtct aaaggtcata
59461 atatgccttc ttaacttact acattctatc ttaagttaat attgtactac ttcacataaa
59521 aagtaataaa ttttcagcaa tataatttta tttgtactca ctcctttttg atattgtgtt
59581 cacatattta tattttatta acctcatacc aaatatcata gttttttatt taaatattta
59641 ttgtctttta aaggtacaga aagaaaaaaa aaaagacagt tgctgggata taatcactta
59701 tctaccgtgt ctggggctct ttattcctta catatatttg agctcccatt tggtataatt
59761 taccttcaac ctgaagaact ttctttagag tgtcttgaag tgcagggcta cgtaggtagc
59821 atatactctc aacttttgtt taccttgaaa tgtctttttt aaccctcatt tcaaaatgat
59881 attcttactg gatatcaaac tctgaattta cagggttttt taatgttcat cattttaaag
59941 ttgtgattct atcagttttg ccatatatta tttctgatga gaaattagcc atcatctgta
60001 tcctgattct ttatatgtag tatatttttg tatgtgttag tggccattct acaagtttct
60061 cttcattttt tggtgtttag aaatttaatt atgatgttct agatatgatt ttctttgcat
60121 ttattgtgct gagagttcac taaacttctg ggataggtta catttagtaa atatagagaa
60181 ttatttcttc aatttttttt accttctttc ctttctcttc ttgatttgag gctcagagaa
60241 tattcaagtt agatcactta atgttgtctc ctaagtcact gaggccttgc ttgttttatt
60301 aagatatttt tactccctga ccttcagatt tgacaatttg tcttgatctg ccttcaagtt
60361 tactgatatt ctgccacctt caaatttttg ttaagtcctt tcagtagctt tttcattcta
60421 gattgcttat tttttctttc cagaatttca attttttgaa ataattttta cttttctgct
60481 gagatttcct atctttttat cagttatgac tttcttttct taaaagttca tgatcacatt
60541 tataatggct tctttaaagt ccttgtttgc taaaatttca acatcttatc atctcagaat
60601 acattgcatt ttcctggttc ctcacatttc tggttacatt tgattatata ttggacttcg
60661 tggttgatac attgttaaga cttgggatta tgtctctgaa attgtatatt ctgaaaagga
60721 ttgatttttg cttcagtgga tagttaaatc actatctgtt ctcttgaatt tgtggagatt
60781 tagctttaaa cattgttaag gcaggtctat ttgatgtaat cttggtccct agggcaaatc
60841 ccttggtttt cgtagtccac atatttctaa gatataacct tctggagttt ctggggaaag
60901 cctgtggttc tcctcaagcc cttctgactt aaaggaactc aaaattcaat ctgtatcttc
60961 cctgtcatag gcaacagttg taatcccctc tttagcacgt ttagccttct agccattact
61021 ctttgctaat tttcctagga tctctcccat atatgtgcag tttaggggtc agctaaggat
61081 tttagaRagt ttatacacag attttggggc tcactcctct gtggcatcct ccttcttgtg
61141 tttccctttt caatttctgg caaccctagc tgattcaaac tccatcctct gacaagttag
61201 ttaataagac tgactttcta cttgagctct gtcgacccca tccccgctga actgaagtgg
61261 gtcctcaatg actaagccta ataaaggagg gctctacccS gtgcagttat tattttcaag
61321 ggtaccctgc agtttctgcc tgctttgggt tgctctccag tcttttctat atattttttt
61381 caatttgatc ctgaattttt aatttttatt ctgatagggc tagtccaata aaagctagta
61441 catcattgct agaattggaa taatttctca aatgctttaa agtactcaga aggctagagg
61501 atctaaccta aattgtttgt aaggaatgga gaagagtaga aataaaaggt ttcttaaggc
61561 agtggtaggg aaaataataa agctatttct atattctata aaatcctaat gaatttggtc
61621 tatttaatac ataagttaca tatagaattg tattggaaag cattaatatc gttattttta
61681 atacaaaatg ggcaatcgtc agtcctcttc cttcttatta aaaccgtgcc ccttcatctt
61741 ctattctttt aaaagtcaca aattcagtgt tccaccttta tctttctaag ggctgtttcc
61801 tgatctgaac ccagattagc aattacgtta aaattagtgt aaagaaaagg cattatacaa
61861 atttattcta tatagatagt ggttaaaatc cttaatgagt tattatgcta ataaactaca
61921 cttgctagat gcaaattaaa cataaaaagc attcaaatta gtatgttgat gttgttttta
61981 atttgtttta tgtgcttaca ctagttagtt taattttgga gacttacttt ttcacctata
62041 aaaatgagga gtccaagtat gttaatggta agatcacttc caaatactat gttttgaaaa
62101 tataagttta tcgtatagca atcatacaca agaaatagaa tgttcttcat aaacaatcaa
62161 tttcaatgta Ytatttgcat gtaagagcat gtcattattg tatgataatt tatagtttag
62221 agggagtcct tttgcaaata aaaaattgag gcttaaagaa gcagacatat gatttttaga
62281 cagaaaaaat ataaaattat tcattggaac taggttttta ttttgttcaa ccggatattt
62341 agtcactctg actaggcttc aggaaaacta ttaagcaggt ggaggagaag aaacctacaa
62401 aaaagactta gttggagtag atagagagat taaaagaaaa ttgagaaaat gggttgtcat
62461 gaatgccaac agaaaataac atttcaagaa ggaaagagca gtcaacatta gaaaatgact
62521 atcaagttaa gggtacacaa cattccctta actgtattct gactgttatc agtagagatg
62581 taagttataa tttttaacaa ttacaaatgt taattcaatt acataatgat actttaataa
62641 aataccataa aataatttag ctttccaaga aaattcatca tattcaaact ttatgaggtg
62701 tatttacccc tataaaccaa aattacaccg ttgaaatggc ataaaaatgt tatgatggag
62761 ctccagaaaa gcaagcttat taWtgttatt atatcttctg ataatataca tacttttgtg
62821 aatctaagta tattttaaat aaatgttgcc actaaatatc tctgtacaag acagtaagaa
62881 ttttaaaatt aacaggatta actcgtgtgc ctttctaagg ggctaccaaa cttaaaagaa
62941 tgtttcacat agtgcaatta tatatatcat aatgattatg caYgtaccat cacattaaag
63001 aagagtcaaa gaagaaatgt tggaaagtga gctctgaatt catatgatta ttccacagtg
63061 attagatctc atgttagaga gctttctcat taaaaccaga agtcagtggt ccagtacata
63121 catactttta agagaggaaa atgcagtcag agagagagag aaaatgattg atatgatgta
63181 catatggagt ctatttaaaa actaataata tagtaaagtc tcctcaagtc cttctctgcc
63241 tcactactct tataattttc tgcaattaat gtgttcttaa aatttatgaa aacacaaatc
63301 tggccaaaag atatgaaaca gataaatgag agatatcttc tgcctagtac aggagaaata
63361 tttaagggtg agttaatgta actgagaaat tattttctcc tagtttagaa gggaagagag
63421 gcccacagag aactttcaat aagaaataaa aagtatatca agtttaggat agaagtacca
63481 aaaaaattaa aaacaaagat aactctgaat tagatacagt tggatgtttg atgatatagt
63541 ttcttctttg gctggctttc aagtcagaag cttttaaagt ggagtattta ggggtattag
63601 aagactttcc aagggcaagc cagtatattc agcacagaaa aatttcaagg tgttgagtga
63661 cattttgaaa tagaaactaa aaacttcctt tttcatatat taatttgttt ggaaaagatt
63721 tctataaagt aaaaaaatac agaaattaaa tcttaatgct aaactctacc tcatttaaac
63781 cataattagt actgacccat ggatatctaa attatttttt taaaaggctc tatccataac
63841 attgtaagct gcatttgtag taaaacttca tttttataga cactgaatcc atagatcgta
63901 tactaatYga atcatgactc aattcagaaa aaaagatttt atacctaaaa ctttatagtc
63961 actggttatt taaaaaggat tgtaatttta atttgtatgc atatttttga tgcaaaaata
64021 ctttggtgat caagaaaaga ctttcaaaca cacatttcta ttgtacttga attacaattg
64081 tgtgaggKtg gggcggaata taaatatagt tcaagcataa aataaaatga tataaatatt
64141 ctacctatta aagcatgagg tcaaatcact atgatattta gattctaaca gatatatttt
64201 taaaagtgac ttgacaatat tattttaagt tatcaatatt tacaacacaa tatacatatg
64261 tcagtgccga cgcccactca ggaaaatgaa aaccattcct aaaattttga aaagaggaga
64321 atttaatatt gtgggttgat cacaaaggcg ctagaaggaa tcaaagagca aaggatgaac
64381 agagtaggac attggaaaag caaaaagaaa aagtaaaaca cagatatcaa gtgttactga
64441 tgtgtcagtt gattgtatct gccagacgta tgccccttga tcagacctgg aattgccaaa
64501 aagttgctgc ccgtgcccta gctgctggga tcaagaatcg cctactgcca ctgctgctgc
64561 agccactgaa ataaccattt aaagcagacg gctcttctct ttctttttct gtctgatatc
64621 ttcagtggtt tccctcaggt ggaaattatg gagaagccag atggcaaagg agcctgggaa
64681 atctggtttg cagattccca tccctataag gctcacagta tatagcagag tagaaaatgg
64741 tatgtatagg tttaagagaa aacaggtaat gatcagtact gtctgcaaat tgcgcccttt
64801 acaattttct aaatgattgc aaaattaata tgtcaattat aaatgtagaa agatacatac
64861 ttaaaaaatt cctttggggt ttatatgaag aaaaaagctt gatggttatt acaatggaag
64921 atttatttta tataaagaac Wtgactggtc gttttggatt taaaaataat ctgttccttc
64981 agcctaattt tacctgagcg tttccaccac ctggaaatta aatctcttca tgctaactga
65041 ttatcaaatR ctaaattata tgacaagaat tttactcccc aaggaaaata gatccaaaaa
65101 tggaagaaag ccagaaccca ggaaaatgga aaaaaaggag gaataagtgg actaaattat
65161 aaatctgtaa aattaggata aagtactata cttagcatgt aagtattaat agtgatgttt
65221 ccatgttaaa atatagcacc attgagagtc ctatctctca atttctaaac ttctagtata
65281 acactaggct tttcttgtca ctgtgcccac ccacgtggtt tcatgagagt aaagaatgtt
65341 tttgaaagta gacatataat attggagtat aataagatac tatttagtaa ttataatgcc
65401 aataatgtca ttttctgaga aagaaaatgc tcccaagact aaacagagta aatcagctgc
65461 ttgggactac acagctttcc taatttttat ggttgacaca aagtttggca attctttagt
65521 tttactaggt aaatgaattc ctatgatggg tattttagtt catcaatgca ttaacaatag
65581 aaagcatata tttaccggga ggctttataa atattttatt ttattatttt atttatttat
65641 ttagagatgg agtcccactc tgttgcccag gctggagttc agtggcgcaa tctcggcttg
65701 ctgcaacctc cgcctcctgg gttcaagcga ctctcctgcc tcagcctccc aagtagctgt
65761 gattacacgc acccaccacc atacccggct aatttttgta tttttagtag agacagagtt
65821 tcgccatgtt ggccaggatg gtcctgaact cctgacctca ggtgatctgc ccgcctcagc
65881 atcctaaact gctagaatta ctggcatgag ccactgcgcc ctgcctataa atatttcaaa
65941 acatatgcca cttaacatgt gttaatcaca tgccatggtc aaagtgctat ggagggatgc
66001 aagtgtgaat aagacataga cattgatcat gaaaacattt tgttgatggt ctctatgtgc
66061 tatgcactgt gctaggcact ggagatacaa agataaataa aacatggttc ttgcccttga
66121 agagatcttg tcttaatgag aaacacagag gttaaactag acacttataa tacaatgcac
66181 taggtgcaat gatagagaag cactaagaaa gcaacaagga aggtagagta aaggtaaatt
66241 tcctgaagga ggagaaatat agcctgatga caaaataaca ggtagtaatt atctaggtaa
66301 agagggattg gcagaaagtg aaggacgtct actgacagac acaagagtag aagtaaaggt
66361 agaaaggtta gaaacagcat ggaaagcagg ccaaaattta tgagaatttt gcagctctcc
66421 aagcaaaaag tgatgagaat ctatcctacc atactaatgt tgtgaagaga atgaaagaca
66481 ggaaatcaga gataaaaagg aggtaggata aacagaattt attggctaaa gtgaggagaa
66541 gggagaagtc tggaataatt catcagttca tagtacaaat acataaaagg gtggttgtgt
66601 aactaattgg gacagagatt gctgaaaaag atttagagga ggaggacagt ttagaatagg
66661 gtgagttgga tatgtagcat tctgaagctc actgtgaaag tgccatagga ggtatacatt
66721 tgggagtcag caacctgtgg gtggtagttg aaatcatgaa agtacatcag attgccaaaa
66781 agaacacatg gagtaagagg atcactgggc taatcttagg atgaaaccca gagaaagctg
66841 tcacttaaga attgacaaaa taagaagaat ttatgaaaaa aaataagaaa gtttcaaaaa
66901 ataagctatg actagcaatg tcagaactat ttgagatgtt gatgataaga actgccaaat
66961 gttcactaga ttggccattt gggagttgtt ggttacccaa gccagaactc ttttggtagc
67021 atagtgggag aaaaagccag actgcaaaag atgatggaaa aacttggatg tgaaaaggtg
67081 aaaatgacat gcatatgtat tattttttca gaaattttag atggagaggg gaagaaagag
67141 ataataattg gaagggtcat tggttttggt agagtttttg tttgttttta agttaaaaca
67201 gagacttgag taaaattata agctaaggaa gaaaaagaaa gaaggaaaat gtaatataag
67261 aaagtcagtt aatatgtgaa ggataaatat cctggaacag attcaactcc atcaaaaatg
67321 attaaactga aaattgattt taatgaggta aagatgttac tttgatactg aatataatat
67381 aatgagaatg tgtatgttaa taatagatag gcaagagaat ggaaaattga caaagatact
67441 ttcttattcc ttcaaattta ttgataaaaa ttggagatta cgtatgttga cagtgatggg
67501 gacatgtatt atgtaacagt ctacaggaga gtggtgaaga ttgaaataga cattgaggaa
67561 agggaaagtg actgctaaat cagaacagat aaaagaatag atcagtggca ctaaagactg
67621 agtggaggtt tatatgaaac tgacactttt ttattgctgc tagtttacac aacagtgtga
67681 ctttcttcag ctgagattga gtgcccaagt ctaggagcag agatgaaaat gttcatatca
67741 aggttgagac atggtggata aacttggtaa gggaattaaa tgagatgaaa gaagagctaa
67801 aatgattaag gaagcatagc catgatcaga gacataaact tggataaaat tttggaaatg
67861 ttaattagaa gatcaaatga agttgaaaaa caggtgaagt atgagtgaag aaatgaaaga
67921 gtaggttaga aagacataac cagagactga aatacgaaag ttctagaatt tgaccttgag
67981 gttcatggca gagtgttaga gaaaacaaaa gaaatgagtt taaaatagct caaaatggag
68041 tgagggaggg agggatgaat agatgaagca taggggattt tcagggcaat aaaactattt
68101 tggatgatac tctaatgatg gatacactgc tgacattata cacttgtcaa aacccataga
68161 aagaatgaac tctaatgtaa actgtacacc ctagttaata atcatgtatt gatattggtt
68221 cattggccag gcaaggtggc tcatgcctgt aatcccaaca ctttgggagg ccaagatggg
68281 aggatccctt gaggccagga gtttgacacc aatctgagca acatagagag acctccacct
68341 ctaaaaaaat aaacattaaa aaaaattagc tagacatggt ggtgcatggc tctagtccga
68401 gctgttgagg aggctgaggt gggaggatcc ctttagctca agagtttgag gctgcagtga
68461 gctgtgatag caccaccgca ctctagcctg gatgacagag tgagatccgt ctccaataat
68521 gattgtaagg ttcattaact gtaacaaatg tacctcacca atgcaacata gtaataatag
68581 ggtaaattat tcacactttc tgctcaattt ttctgaaaac ctaaaactgc tttagaaaat
68641 aatatctatc tgaatcatga cccaaaatga ttgttgtaag gaatatctac attgattctg
68701 aaatatatta ggaggttggt cagtttttgt gtgaagagaa acatttagcc aggtctcaaa
68761 gttttcagag aaggagaaga agatgctatt gtgaggtttg tctggtggag gtgatgagac
68821 ctcagaaagc acatgtaatt gacaacataa aacaaaacaa aacaagaatg gagatagcat
68881 gaatagagtc tcattaataa ataacaaaaa acaggttcct tgagtatcta agaaaggcaa
68941 aattccatgg ccYctaataa atagtgaaaa tgatggaaga gaattcccac gccatgtcag
69001 ctcaaaacaa gtatattttt tgcccaagcc atgctcaagc tcttgctctt tttctatgcc
69061 tcctgtgctc atccccactc ctattgtcta ggatcccgca cactgggtta ttgatgtagc
69121 tagtcatgac acttggctga tggagttcat ttcaataccc tagtagctaa tgtcaataga
69181 actttcattg attttccagt gtcctttaat tcgttaggta ttgacctttt atttaaatcc
69241 cacatgacca ttccaaacat tttccacact tttcaagcct caatttctag tcccttcatt
69301 ttttacaccc tcgtcatgtt cagttgttaa actctatggt ttactgcaaa agctgtgaga
69361 atctagcgta agtcccttgt gttcttgata ttcaacctca aaatccttcc atgattctct
69421 catctttagt gacatcaaag gcattagtta tggatactgt tgacacattt aatacccatg
69481 tgagcctaaa agagttttga atcctctctg agtcacaatg tccacatcag tggaatgaag
69541 ctattgtgaa gatgaaccaa gaaaatgaat tcaagaatgt gttgcaggaa aaacaaatag
69601 atcttgttgt atttgttgac tcggatagat tggtaaaact tcctcaggtt ctgtgtttta
69661 aaaatgtaac agatccaaca catatttgga aggtagtctg ataggatttg ctgtggggaa
69721 gtgagagaaa gaccaaagcc gagaataatg gtttccagct tcatccatgt ccctaaggag
69781 atatacctaa tgtaaatgtc gagttaatgg ttgaagcaca ccaacatggc acatgtatac
69841 atatgtaaca aacctgcaca ttgtgcacat gtaccctaga acttaaagta taataaaaat
69901 atataaaaaa aatttaaaaa agaccaaagc caacaatctc ttctaaactc atcccttttt
69961 aattttgtct tttcagccac agcagcagaa aacaaaaaca aaaacaaaaa aaaacagcct
70021 ttatttccat tttccccagc tccactcact atcttgttcc tagccacaat tatctcttct
70081 ctggattgta gcYgtagagg tccaattact ctccctttcc actcttgact gccttcaata
70141 tattttaaca cagcagccag agtgatccag tcattttcct gctagaagac ttttgatggg
70201 tttccatccc actcagagta aaaactgtcc ttaaaattgt ctacataatc tgcccaatca
70261 attccctttg tgatctcttt atctctccct ctgtcaccat gctccaagct tatggtaccc
70321 cttgtacttc cttatactca ttgaacttgg ccttttcaat ttctgtctct cctatctgcc
70381 taaaatgata tttctctgtc cacctcattt ttttttcagc atttgcttaa agcttattag
70441 agaggactgc cattgccacc ctcttcaaaa gaacaacttc catcctttgt actacctatc
70501 tctcttattc tgttttattg ttttttataa cacttacaat tattttatta tgtactgact
70561 tgttcatttg tcttcctctg gtaaaatgta agctgaagtt ggtctgagat ttcatatatt
70621 tggtttacta ctgaagcctc aatatctaga aaatatttgt acataaaaga atgaatggtg
70681 gccacatggc atacattagg taataacact cccctaatta ataaccattc aatgggatct
70741 cattgctctt taataaaatc aattaaccta ctatagccta ctaaatttgg actttatata
70801 aaatgttttt atctttgttg aaagactaca ctttgagctt ttgttcctat tattttaaat
70861 attttgtcca ttaattaaac agcttgttat attcagctac tctcacttta cttcaagtta
70921 attgagaatg gtaccattta ttggcacaga agaaatgaat gaaggaaata tttggccagg
70981 agttgcagaa tgcggaatca aaactactat tttagaaatc agtaataata cccagatgct
71041 attctaaagc tttacaggta ttgactcctt acatgctctt actaatttga tgaaatagat
71101 agttaatatg cccattatgt agatttaaga catcaaggct tagagaattt aaataccttg
71161 ctcaaggtca catagccaat aactggaaga accaggatat tagcttaaat tgtttgattc
71221 cattctctta acaactatgg atatacctct ttggagctat ccaaatggaa aatgaaataa
71281 gtaatctgat atatgagtct ggagttcKgg taaggatatg gaatggacac atgcatttga
71341 ggccagtctg catatagatg aagtttaaat tgcccctcta cctttcttta tccctacttc
71401 tcttttattt tcagtctctt tcaaattctt tgctccttcc ccatttctta aaaaaggtat
71461 tctctatctt gaaaataacc tgctcatgta ttctaagcta aattaattag gtgtctttgg
71521 ttgcaaatga caatccaatt gaagtgaaaa gataatttaa ttctcacaaa actggcctat
71581 tcagggatga gatccaggct cctttcatca ggctttggta tcgcttgctc catctctcag
71641 ctgtgatttt tttgtgtgtg tgttgcccca gttctcaggc aggctctcct ctcttggcag
71701 taaagtgact tttagtagct cttaggctta gatttcatcc tcttatctat cccaacataa
71761 acatagattc tcttttacct gcagttccaa tacagagcct cagctttaac attcactggc
71821 tctgactatg aagtattgtg ttcaagagga ggcagtgttc tcaatgccag gcctacacca
71881 cttgcccagg tggagcactg agagtagggg tagctacacg caaaacacct agagcaagcc
71941 tcggggagta gtgaatccca aggaaaagta ggatgctgtt atcagaggaa ggggaagtag
72001 ataccaaaca ggaaaaacaa aagatatcct tcaggggctt aaaaacaata gaataagtta
72061 caggaaacta tcaatagtta catgtacaca aaaatttaaa tcgacagtaa tacaaaatca
72121 taatgaacta aattaaacta caaacaaaat gctagaaaat atttgtaaaa gtgtaatggt
72181 gacttgtttg tgtcaccgct tcactgctac atgaagtaaa aatcaacaag aaaatactaa
72241 gaccccactg gatagatata aaagatatga acttagagtt tcaaaaggag aaactagtag
72301 gcagtataaa tccgaaagca aatatattgc aatggagaag ggcagttaat cattttttag
72361 aaagttaagt ttatgaaata tgattgatat atattttatt ttacaaaata atgtaaatgt
72421 gttatatatg cttggaatta ttttatatgt tgtatttcat aataaagtag aacatcacca
72481 gaatttgatg acatacttgt catcaataat agatttgaca acactgcaat taacacaaaa
72541 cccacaagga atttaaaagc atagcttgaa actgaccaaa taacaactat actagcactg
72601 atatagagct tacttattcc tgacacagtc tcacatacta tgtatttatt acctctcagt
72661 actcacaatg ataagtacta ctattatctc aatgttgtag atgatgaaac agagagaggt
72721 gaagtaactt gcccatagtc acatagccaa gttgtagaga attaattatt caaattcagg
72781 aagtttggct ccagcatcca agtgcttaac tcctttacca gtttccagag aaaaacagtt
72841 ttgtctgcaa agcatttaaa taaacccagt tacatgtcag tgagaaaaac aatactcaaa
72901 cagttcagag ctgttttttt ttaaacctgc atatttgaag aaggttcaga taggagttca
72961 gacagagtat gagtagaaga caaaacataa gattacatag agaaagatWt ggggtgagat
73021 gtaaaagatt tgcatttcta aacttctcga aattcacact tctttctcaa acacctgtgt
73081 cttctttcca taaaaaatta aaacttttag aaaggactgc aatatactgt acagtctata
73141 tctcacttcc ctcttgcatt taatcacact ccatttagat gacattcagc tttaatctgc
73201 ctagactgcc tgtactcact tcatttctca accctagcta aacttgcact ttgtcccata
73261 aggctttcaa actactccca tctcttagta atctcgtcct ctgagaacac ctattccact
73321 tcttttcaaa ttacctttgt gtaatgttac acatctatct atatatatct acatctatat
73381 ctataaatgg gcatatatac aatctcagga agagagaacg agagagatgg agggagagag
73441 agaccttctt tttgcatagt ccctgttttg gaaatcagaa gactaaggtt atgtttactt
73501 tgtatgccaa gcacacagca gaatgcccta aattcatcca tttatctgca catgtttttg
73561 aatgcatgtt gtattctggg cactgtgcct ggcatcatag atacaaagga gtggaagata
73621 cttttcctcg gccttcaaag atctctaagt gcacacgaaa aacaaatgtg tagataaata
73681 attaaaacac agtgtgaaaa atgccatagg gtaatacata cagggtgcca tggcaaccca
73741 ggggccgaat ggcagcttct gcctggggag ttgaaaagat ttcacagaag aggagaaatt
73801 taagctaggt cttcagatga gtaggagtat aagaggcaac aatagggata attataaaag
73861 catgtttgcc ttgataaata gtttgcactt tatcatttag ccagtacaaa ttataaaaga
73921 gttttacaaa agagaaaaat gtgatttaac ttacgtttaa gaaagatagc tgaggtaggc
73981 ctgagaatgc attggagtga gRaagagaaa aaaacaaatg gctttttcaa caatctaggg
74041 aggaataatg gaatgtctac attaaaacaa cagtaacagg agactaacaa aaagaatgag
74101 tctgaggaaa gttaatattc tttattttat tttattatta ttatacttta agttttaggg
74161 tacatgtgca caatgtgcaa gtttgttaca tatgtataca tgtgccatgt tggtgtgctg
74221 cacccattaa cttgtcattt agcattaggt atatctccta atgctatccc tccccccatc
74281 ccccacccca caaYagtccc cggagtgtga tgttcccctt cccgtgtcca tgtgttctca
74341 ttgttcaatt cccacctatg agtgagaata tgcggtgttt ggttttttgt ccttgtgata
74401 gtttgctgag aatgatggtt tccactttca ttcatgtccc tacaaaggac atgaattcat
74461 cattttttat ggctgcatag tattccatgg tgtatatgtg ccacattttc ttaatccagt
74521 ctatcgttgt tggacatttg ggttggttcc aagtctttgc tattgtgaag agtgcagcta
74581 taaacatatg tgtgcatgtg tctttatagc agcatgattt acaatccttt gggtatatac
74641 ccagtaatgt gatggctggg tcaaatggta tttctagttc tagatccctg aggaatcgcc
74701 acactgactt ccacattcta tggcataata atagaaattg ataacttgga ggagaaaaga
74761 aggaagggtt gagacttggt gagaaattta gcagaggaaa atgagcagat ggacactcca
74821 caacaaaaat aggggatgca gaaggaaaag cagattggaa aagggcaaca gggacacaSa
74881 atttggctat aggcattttg attttgtggt acctgaggaa cattctcatg gaattgtcta
74941 ggaacaatta gtcagtgagc atctggaacc caagtgaata atgttgagaa tttctaaaag
75001 tttaggaaac ataggtattt ccatcacttc cattataaaa acttcattgg gaatgttgga
75061 ttaataggaa gatcatgcac agaatgatta tttaaggtcc taaattgttc cacctgagca
75121 tgggtaagga gaacaggtgt taattatttt tgctgcgagg ggaaataatt taaaaactag
75181 agggaggaga gggttaactt tgggaagaga gttcagagaa cctgagaatg tgatgagttg
75241 tccccttttc cacccacact ggagaggggc atgccagggg cagttgggaa ggatcaggca
75301 ttcttcccat aaagtcgagc ctatctctct atgccagcag aacatgctgc agggaggcac
75361 aattcccacc attctgaccc accatttcct aggtagtcaa acttagggtg gcaagaacac
75421 ctaagattcc cacagtctag agaaggagga agagctggca aggacaaaga ccagaggaaa
75481 tggcctcctt gtgcagggag agcccaccac ccactgggat caggagaagg tcctgttatg
75541 aggagcaacg cttgtgaatg aagaagggtt ctcagctggg tgcctgaaag ccatcaaaaa
75601 tgttccatga tcacacaaaa gagtcttaca tattttcatt actgcaaacc agccttgaaa
75661 atatatgtat gggccaagaa aactttgccc accgaagaag aacctccctg ctacaacaca
75721 cctgccagtg actgcccagt gataaatgct tgcaggcctt gtgaaatgag gagatagctt
75781 tgcaaagggg accagagtaa aatacgtaaa aagaagggaa gcccttatag agactttaaa
75841 ttggacagtg gctaagactt tcattcttac atgagattaa acaagtaata atagacagat
75901 tgagtttccc agagtatgtc agctgtacac aggcaggaag ttaaaaaaaa tacatgtggt
75961 cataagaata catgtttgca cgtctgaatg gtaagtgttc aaaatccact ggatatgcat
76021 ataggatgaa actaaaatat agatatggga gatcatacaa cctgtttgta tataagtaca
76081 agaggaaaac atggccaaaa atatttgttg ttgataagga aggattttaa cactaatgaa
76141 ggaaaaggat aacgagagat ctgtgaaagg aaataaatta tgtagatgca aatgacttag
76201 tgaatggctg taggagcaat tttctaaatt tgcaggtgat atttaattgg cagacattgc
76261 aaacagcaag gagcaattca atcagataaa gaacttgaat cattaagtac ttggcctaat
76321 agatggcaag cgcagataag tggtgaataa taagtcttga atcaaaacta aagaacagag
76381 agtgaacatt tgccaaaggg aatgatacca tccaggaatg gctcttagat ttaaggagaa
76441 cttatagaca aggacaaaca aaacttcagt aaaatattac aaataaaaag atttggtggg
76501 ttacagctta tccagctgaa gctgtgatga gaaaatctga caacttaagg atagataaat
76561 cctgaaatcc acggttggct attgaaattc catttgttac ctagaatttc atctatccct
76621 gatcatggtt tggagataaa attaatgagt aagataacat ccaagcatat aagaggttac
76681 agatttgggt gaggatacta taaataactt ttaaagacat ttcggatatg aaattctcta
76741 tgttctatgg gcataattgt gaggtattgt gtttagattg cttggtcatt tttatttaac
76801 atgccagttc tagtctacgt tagtctggaa attataaaca tagagaaata tgctacaagt
76861 aaaagagaat aataaaaatt tattatagcc tagaaaggta gttgatgcaa tatataatat
76921 atttcctctt tctctKtttt tgcaatggtg aatactttac taaatgaaaa ggttaatttt
76981 aatattaaag agaaatggaa acatccttta gtacaacatt aaaaccacaa atggaacctt
77041 taaatattca ctcactctag caatcaatgt tccaacctca tttaaaggat tttttttaac
77101 ttgacataca aatattcaga atgaatattt ggaagcagag ccacgagcca taccttgaaa
77161 gataactgcg gacttactct tgcctgccat ttggRtactt cagatttcag ttgttctaag
77221 tctcaatgat cctgagtttt ctttttgtaa tttggtaatt ttgtaatggc aggctttaaa
77281 ataagtaaag gttttcttaa ctaagaacaa aaaaaaatcc ggagacctaa caaggctcag
77341 ctgtgtccag gtaggagatg actgcagttt ggcttcagag gctccctcta gcagtgtgag
77401 gacatctgag tgtctctcct actcatttta tatttcattt gatactgcac agaatcacta
77461 ggagaacagc cagaattgat ttacttctca tagagacaaa ctgtttctgg gagctagtta
77521 tcttcttatt aacaaagagc ttacattccc ctcccctcca gagctcctaa aacatctata
77581 taatagctac attaagtgaa ttctacaatg attgaaaatt ttactataat gaagataaaa
77641 attcactcaa ttctagttct tttatccttg atttgcattt gaYtgaaatg tgtatttgca
77701 gtttgggcat ttcagtcagc gattgctcct gtattctttg cccataagca gccttcagat
77761 ggcctcttag aaaattgaag aagtctctac atgactggaa aactctttct gatggtctgt
77821 ttcttcaaag atatagcatt acctcttggg aattatagaa acaatatata ttgttaagcc
77881 tcttctaatg ctactgtaat ttataactgg taactttttc ccccacttag cattcaataa
77941 gcatttattg gggcctaatg tgtaggtgga attttggaga atacataaag tatagaacac
78001 agagttcttg ttcttcaaga gctaagcagc tctatagaga agagagagac agaacgacag
78061 agacagagac agagagagag agagaagaga tcaacacata tccaccactt gctttactat
78121 ctatacttca acttttggtg gactcatccc atcttatgga ttcaattatc atctataagt
78181 agaccacttc cccaattgtt tctttaaccc agacctctca cctgaactac aggcttttat
78241 tcctcacaat gccttcagca ttttccattt ctaatgggta ccYcaaacat taagtgttca
78301 aaactgaatt tctgatttca cccctttccc Ycatccttgt ctgttgtagt gtatggcaac
78361 tccaattttc aagttactca gtgcaaaacc tttgagttgt ccttgaattc tcttctctct
78421 gtcacgcctt atatacaatg catcagcaaa tttgtctggc tgtaaatctg aaatatacca
78481 taaatataaa tacgatcact tctcaccact cgaaagccac tatttttctc tctttaggac
78541 tatttcaatg gccctttaac tgctcaccaa gctactgctc tttttcctat ttctgttaaa
78601 aaatctattt ttaacagagc agccaaagtg attgttttaa aatgtcagat cgtattacac
78661 tctccctata accctccaat agttttcctt caactctaat ttacataatt ccctataatg
78721 ccttatatgt tcaattatat acatatacac ttaccaccat ctctgtgaaa tctctttctc
78781 ctacttccct agtatgagta ttgcttaggt ataaatcaga actcagaaaa ataagagtaa
78841 gaattaaaaa ggacatatgt gctgaaaaaa atagagcaat gaataaatac tggggaagta
78901 attagaagga agagattata cagaaaccat aaggcaaaaa gttaatcaaa tttatcaagc
78961 atattatagg ggacatggcc aactccacaa tttgttcacc tataatctca ggcaagctat
79021 ttaccttgtc tgagtcttag atttggcctc agcgaaagga aagtggtgat acatcatgag
79081 cagggttgca gcacagctgg cataaatgga ggtttatccc acacatgtgg gctgaaaata
79141 ttagaatcca agctaccaag gtcagagtgc ctggctaaga gcttcatatg gaaaaaaaac
79201 ctttaagtca atgacaaaat gggagagact tttttttttc aggcagtata tagccaagaa
79261 tctattcctt gaatgctgga attgaaggtt atataaaagc acttattact accaaattgc
79321 aaagaacaag cagagcaagt aagttgagag agagagagag aSatctttct tggagaacat
79381 ttttggaaaa tgactgtggg caaagcaatg tgaaaatatt tagggagaca gaagactacg
79441 aagataaaat acctatatca agaagtttat attgaaattg agaatataaa acagatatta
79501 tgggataagt cccataggag agatacagaa aaacagagaa atgaaggata actgtaaact
79561 ctagggaggc aatgtcacaa actcaggtga aaatacatta gaaaaggaaa actaggtatt
79621 tttcagtata tataatcagg ctggaagata aaagctggtt cagagactag tgaaaacaaa
79681 tcaggctgga agataaaagc tggttcagag actagtgaaa actaaattgt tcctataagg
79741 actttaaaag gagatcagca tgtgagtaga cttggaggcc tgctaaagag ggagctagga
79801 gaaggaatag cacgtggttt ctgagaaaca gtgtagcaat aaggcttatc acacataaac
79861 taaactaatc atgatgtttt tgtcactatt gtgctgtcag agaaaaaaat ctattgaaat
79921 aatgaaacat actaattttg agaaacatca gtgtggcaaa acccaagaaa cacttttcaa
79981 atgaatatac tcaaattgca agatcaaaca tctattaatt ataataggta agaaaaagga
80041 aacattcaaa aaagtccttc aaagcgaaaa tatgtaaatt agagtacctt cataatcaat
80101 aaacacttat tgagcatcta ttcatgtgct atcaatgtgt taaaaataca aaagtgacaa
80161 aaataattat tgctcaaaat tctgtgcagt atttagaaag attgggtagg gataaacatg
80221 tatttctcta agattacatt acattatgat aaggtttatg gaaaaaatgt acatacctat
80281 gtatatgtaa aaatagaaaa gagtctaaaa gaactttttc aatctcaaaa cagtgtttac
80341 atatgaggaa atgtagYgat gactgttcat tgtttataaa tatctgttct ctcctttctt
80401 agtaatacag ctcctgaatc ataatagcca gatacagact tcccacaata tgaatttccc
80461 agtttctctc cttagctagt gtggtcatgt gactaaattg tagccaatga tatttaagca
80521 gaaatatcat gtgatgcctc tgagaaatgt tcttaaagga aggattttta ttcttattct
80581 tatcttcctc ctttcttctg gctggaatgt taataggatg acttgtgttc aagcagccct
80641 ctaaagacat gaRttggaag ctatgtactg aggagcacag agcaacaaga gaaaataagt
80701 aaaataagtt tggttcttag ctctggaagc gtgataaacc tgggatagtc agccagggaa
80761 cttctttcat gttgattttt aagccagtga tattttgggc atttctttta cctgcaggaa
80821 gaaaataggc tgattttata taatttgacc ctttaaaaac tagaatatat tcatacatag
80881 tttttacagt tcagtgttta aacttattta tttttcatct tagttttgct tccctatgtt
80941 ttgtaaatgg taaacttatt agccattctg tggtatataa aagtttcact tttatgtttt
81001 gtttttatat tggcccaact tccatgttca gctaaataca ctaacataat ttaaattact
81061 gcatttcatt gggaatccaa gttagaaaat tccttttccc acatttttaa tacctaaaac
81121 ttgggaaatg aataacatta taagtacttg aaaataatat tcactgattt atcatgtgta
81181 agccctgtcg aaatatttat tttctactga gcttggaaga tattttaaaa tgattccatt
81241 aaagaaattt ttaaaatttt tgaaatatct atttacttaa gatgttgaag aaaacataag
81301 catctacctt taccttccat tttttagatc tcatatgata tgacaaataa atagaaaatt
81361 agaggaaatt ttacaacagt actctaaatt aagtaagggt accctccatg aaccaggaat
81421 tggcagattt caaaaaagca cactcctaag aaatttctgt ttttcacgtg accctgacaa
81481 aatccattaa ttgtcctaat ttggaagggt acaaactgca gaaaagagca gggtattagc
81541 tcagcaggaa gcataatatt tcattgccaa gttgctgttg tgactcagta gcagcacttc
81601 tgttatcttc tgcatggctc ctcactcttc agcgagcaaa ttaggactca ttcacatggt
81661 gatccagggt tcctggcaca gcaagataga tggcaagcca ttgtgccagc cctccaatgc
81721 tctgtttgta tcacattgct tttctcccat tgatcaaagc aagttcctgg ctaatcccaa
81781 agttaaagga tagggaaata gactgcacca attgttggaa gagctgaaca gtacattgca
81841 aaggggcagg aataccagaa taggaagaac tttgtggcta tcttatgtaa tctgccgtgc
81901 tgatcagtta gactaataaa ccaagagaag aaatgaagga taataaattc aaatacctaa
81961 tagagatcag gctgttaagg tataaaagta aagttgggta tagtacctat gcacacaatg
82021 tgcttgaatt gtgagagcta aagtgaattg aagatctcat gaactttcca agggggcact
82081 gtctctcaaa ttcaacaagt tgcttgaaac agactccagc aattttcaca tgaaattgta
82141 agtttgaatt tctatgtaga ctctactaat tttgaattct ggctgaagta ttttaaaact
82201 gtggttgaac ataaccacat ctgtaagtta cttatgaatc atggaccatc aacatgtgag
82261 ttttgataga gaagactgaa aggataacat tttacaactc tattaaatat aatataaata
82321 gtacaattaa tatatatttt atataaattt gtgtcctata gacactgaag acccataggt
82381 taattacaaa aattgataat acatcatgtg cctccaactc caaaatttat atacaacata
82441 agtaatacct aacaaattac cagattaaaa cacaataaaa ttaagatgaa taacatttta
82501 aacaaagaca aaatctcaaa ccacttggaa gtaaaaatag caaatgagca aaaactctca
82561 tacactgttc ctagagcaaa gaggaaataa aaactaaaag tatagactat ttagaaaata
82621 atgataacaa aagcactaca tgtaaattgt ttttaaaact ttgcttacag aaacgtgttc
82681 atctttaaac tctacttaat tactaaatta aaataatgaa tcaagccttc aactcaataa
82741 acaaaatatg aagccattaa agcttgagtt aaaatgcatt aaaatctgta ttttagaatt
82801 cataaatcaa tagggttaat tgaagggaaa aaacagttct ttgaaaagat ataaaacaaa
82861 aacaagcctt gggcatgtct ggttttggaa aatgaaatgc ataaataaaa gtatcaaaaa
82921 catgtgtaca ccacaattaa gaaaaatttt cacaagcatg atggaacagt aagtacaact
82981 ttatcgtaat tatttgaaag aaacgtatgg catgaattat tatcaaggaa aacatcccat
83041 cattaaaatt gacccaagaa gaaatagaag acaattaaat atatgtccaa tatgacaaaa
83101 aatgttattt gtttacaaac aaattaataa tgttatctcc tgcctgtttt cttaatttca
83161 ggaaagagta ggccaaatga ttaagacatt tcataaaagt taYccaatta attctgaaga
83221 gctagagtaa ttcccaaaag aatggctgaa aatgaaatca catacaaaaa aagaaaaaac
83281 attcaatatc actttagtag tggtaataaa aatacttatt aagtgataat tgaattcttt
83341 tacaaaattc ttttggatat gaatatccaa aaatcttaaa tgtcctttca tatttaatcc
83401 gtcagcatat tagaacatag taaagtatta ccaaacaggg tttattacag aatgagcttc
83461 attcaaccaa aagataacag gagaatcttt ggcaaaatat ctaatagtaa gcaaagttgg
83521 agacatggat tagataataa tatgaaatca tcatgtgttc taaaaatgaa agaatggtat
83581 taaaaaatca aaggaaaagg ggaaagtagg ggaaaatagg atatcgtaat gcactttagg
83641 gaataagtgg aagtaacaga ttttatttaa aattttcaaa acgttaactg gaaaaaaagg
83701 agtaaaagca taataaaatt tttaaatatt gaggttaaaa actataataa aagtgcaaat
83761 ctttctagat agtaaaagaa aaacctacca aagaaaacat gctacataga aaaacacagt
83821 aaatataaca aaatctacac aataataaat aacataaaga atagattaaa taagatacat
83881 ttttgggata tggatcaaac actacaccct cataatatgc cattttctca agcatacatg
83941 gaacattgac aaaagtcaat catatattga ttggcctcaa agaaaacact attaagttcc
84001 atttaagtac tttattacaa atagtgttcc ctcatcaaaa tacaataaaa ctagaaattt
84061 agaagaacag taagagaagg cttttcacct gaaattaaga aatcatcatg ttaaacaact
84121 gtagaaaaga gaaaaagaaa caaaaattat ctttaaaatt agtggtaata aaaatactat
84181 atatcagaat ctgtaaagga tacttaaagg cgtaattagt agtcttggcc ctccccttcc
84241 cttcccttcc cttcccttcc cttcccttcc cttcccttcc cttcccttcc cttcccttcc
84301 cttccccttc ccttcccctt cccttcccct tcccttccct tccccttccc ttcccttccc
84361 ttccccttcc cttccccttc cccttccctt tcccttcctt cttttcttct ttccttcctt
84421 cttctccatc tccctcctat ttttcctcct tccctttttt ttccaccctt tcttccttcc
84481 tttagcattt cttcaatcct tactttctcc caagcaaacc tggaatcttt acattgtaat
84541 attatttaaa agccaaataa aatcaaataa tatttaagaa aactatcaag aaatttcaaa
84601 tatttacaaa aatttattct cttgaacagc aagagaagga aacagtttag taagttagat
84661 aggcagaagt agacctagta cttgtgaaat gtgtgccaaa actcttcagt aactattttc
84721 cttctgcttt ctgtagaaaa tatttaaagt tgaaatgaaa gcatttcctg aagtgatacg
84781 tgaattacta caagcaatat gtacaaaaca tgaaaaacag aggtgatatt tagtttggaa
84841 ttgtcatatt caaaatcaga aataaaattt tcattctgat agagcagtga gtttgatttt
84901 attctcagat taaattacag aatggattat taatcagatg tgttttagat gattaaaata
84961 gcagttcatg tttttgatag cattagctat tcaggatcaa atgaatatct gattaaatat
85021 gtattttgag ttcaataatg ccttttacaa agcactUaa accctataga caaacgtggc
85081 ttgatggcag tgtagtaagg aatatttata ggtgattgaa aagctatatc taaagtttta
85141 actagtgagc tatcatcaaa gtgcatagaa ctttagaggc atattgccat actttgaact
85201 tgccaatgta tgtttcatca atactttact agtaatgtga atgaagaatt aaaggcttgc
85261 tcctcaactt ctgaatggct caaacatggt aaaataaagt tgacataatg aatgagaagt
85321 atagattaca gtagatgtga aaaatacaaa agaaccaaag aatgaaatta aagagacaaa
85381 tataaaatct aacatttgct tcaaYtgcat aagactttgt gggctactag tttgatagtg
85441 tttattttaa aaatattttt aaaattttat ttgaccttaa atttagcata aggcagtatt
85501 aaaaatcacc taataaaaat gatacagcct ccaattttat ttaaaaagtg tataatttat
85561 atcaattgag gaaactgatt atttagactg tgttcacagg agaatgacaa gtattttgag
85621 ggacagaaaa tcatatcttt ggaaaaagtt tgaaagcttg tcatagggca gatctattta
85681 gattgataac ataatgtatg gatgacaaaa ctaaaacatc taataaagag tacagggaag
85741 caggtattga ttcattataa aatcaaagtt ttataataat tagaatattt caaatgcaaa
85801 attttcatct tagaaaaagc aactggactt ggtgtaatgt taaaataatg catgtaaaac
85861 acatgatact tggtatatag taagcactaa ctaaatgcat actattaatc ttaccaatat
85921 aatgttctta gcattatatt caagaataaa ttagatcttt agttgttaca tataatcatt
85981 tattttagat taaatttaga gtaaatgtat ttcaatttgt ctaagcttct aagattcagt
86041 gattatattg cttattagtc atgctccatt gtatcatcca tccatatcct tttcatgact
86101 tgagagaatg caaatcttgt atcttctctc aaggaaaata ttatgactat ataattaaaa
86161 taattttaaa aatgtattta tattccagtt ttagtgctag tcctaatcaa aatttaaata
86221 atcattttac aataaatatt tgacatataa tattttgtaa tatattgaat tttctatagt
86281 agatgcctaa ttatcttagc tatttgcaaa taatgtaagt gtaaatatac tgataaatca
86341 agaaccttac tttaattatt gacataatta ttcaggatat taatctttta tcattgctat
86401 aaaacagtca attttcatat tgccattttc tttttattat Ktaaatacct ctctctgttg
86461 ggaatgcaga caaaaagatg gaagagattt ataataatta ggtaatataa gtttagtagg
86521 ggctccatct ttatttattc atatattcat agcaagtatt tattgaccat gaattctatg
86581 ccagaaaatg tgatatgtaa tttttttaaa agtcacaatc cttgctttca tggagcttat
86641 ggcctagtgg aggagtcaaa cattattgaa aagatcatat aaacatatgt aagattgcag
86701 ctgtcatagt ggctaggaaa gtgaggaatg taaacttctg atagtgtaaa caaattattg
86761 ttccaatcaa agagatagtg gatagcttcc ctgtgaaaat gaaaatcaag ctgacatata
86821 gatataaatg agtgtttact tgaagtttca ggagggtcaa tccagactgt gggaacagca
86881 tgtacaaaga ccatgcatca ggaagaactg ctggaaggga aaatggtaca gtatgaatat
86341 gttgaagcag gttggggata gcctacRtaa aggtgtgtag accatgtttt taatctttct
87001 cctaagagcc atgcaaattc attgaaaatt tttaaagtgt ggcaggagac aggatcagat
87061 ttgctctata gaaagactac tttggctaca atagggagac tgaagaggaa gaaaggtaga
87121 Yttcaggtag tcttacaagg agctggagcc taggaaaaca atgaaagctg gaaatagtaa
87181 agaaatgaat ggatattagg atgctcagga ggtaaaatca ataatgatag atttgatagg
87241 gggcataggc tggagaagat aatgaagcta acaagtttta tgacttatat aaaaagacac
87301 cattaaagca agagatgttg gaagaggact ggttgaatat cataagtatg atgttggcca
87361 tgttaaatgt taaatttgag gtatttttaa gtcatttagt agaaatgtca attatgtagt
87421 tgaataaatc agtctagatc ccagagctca gatacacatc tagaaataat acatttgtaa
87481 attgtgtgta gatggggaca atagcagtaa tgaacacaga tggaattgcc taggcagaag
87541 agctcaaatg agaaaaggat gtaggcccaa gtccagagaa ctgctaacat gagcttgaaa
87601 aaggaactga gaaagaggtg tatgttatgt catgaaattc aagggaaagt tgtgtttgtg
87661 atggagggag aaatcatcaa tgctaaatgt caagagacct gattagatga gctttgaagt
87721 atatccacta cgttcaacaa tacaataatt aagaagtact ttttccatga ggaatagggt
87781 atccaacaat atggggaaaa agagttttct cagagtgact gggatatata aaaatggaga
87841 gaaaatgtgg taatgtggag taaccagaga gtagagggaa gggctcaatc tctttgattt
87901 cattttaaca agaaacaaag aatcttttaa tgggaaatct agttaatagg atgatgttga
87961 atgcatgaga caggaactta agtatctgaa atagagagag attgatacat ttaatgatgg
88021 aaggttagcc ttaggaaagg ggacagctcc tgtattgtgg aagcagataa atagagtaga
88081 ctgggtatgg gctataggtt ggaacacaga aataagtatg atttttaatc tcccatcttc
88141 tatgttctct atataataac agtaggatta aagatgtgac aagtcgttgt aaaaagtgga
88201 caagtgagtt gaccaaataa ataaaaacct aagaagggta agaataaata attgtctttg
88261 attatttctg gttatgttgt ttattgatat ttaggaagtt ttagaactgt ctttctgaat
88321 tagtggtcct tggccttttt ttttttatta tactttaagt tttagggtac atgtgcacaa
88381 tgtgcaggtt agttacatat gtatacatgt gccatgttgg tgtgctgcac ccagtaactc
88441 gtcatttaac attagatata tctccaaatg ctatccctcc ccctcccccc accccacaac
88501 aggccccggt gtgtgatgtt cgccttcctg tgtccatgtg ttctcattgt tcaattccca
88561 cctatgagtg agaacatgcg gtgtttggtt ttctgtcctt gcttggcctt tttttaaatt
88621 ttgcttcagt gcagcacctc taatataagt aaggactacc acatactcat tatccattac
88681 agtgttctgc tgtatcattg attctgaagg atggagtgaa gcacctcttt aggaagacta
88741 tacaaaactc agagatggga aagagagaga gagagagaga gagagagaga gagagagaga
88801 gagagtgtgt gtatttgtta gaaaaagttc attatatgat tctgatatgc ctcccacagg
88861 gaggctagaa atttctcctt gaaaatagaa tagccaaatg ttgaaaatag accagaattt
88921 aggaatttgt ttatagatat atgacaagta attgttaaat ttaataaata gtgtagcaca
88981 tacaaataat cagctccctt ttcctaaata aaaaacttta gctattttta ttctatttta
89041 aaaattcact tcgtgatatt taaaggtatc cattattaaa atcagtaacc attccaattt
89101 ataatcattt aaaagtaaaa catataatta tttattagaa aaatatttgt gaacatttta
89161 taaatattat caacatgttg tcagtaaatt cctcctcagg tggaattttc tgactttggt
89221 tgagtcatta aatgttttgg gtcactagtg atcaattagt aaattactga tccatagtac
89281 tttaatggcc tgaaaaagta aatgcatctt ctgagcacct aattgaatct tagttattta
89341 ttcatcatta aaatcaagat aaatgagtca gctcacaata atgaaactga acagttacat
89401 tcatttcaca aatagattaa actaaagctc agaaaagacg ggacaaggga gttaaacatc
89461 caggttagat tttccatctg aaaagaccca attcccaata tcttgctata aaaattaaat
89521 acatatatga gtttgaaagc aatatttttc tgcaagttca ttttcatata gcgttattaa
89581 gtttaatacc attcagttaa taattgtaga tccatgRtac gtatactaat atggtcttgt
89641 tataatgtaa atagtacagt ttaaacagca aaatttcaca ctgttttaat ataaaaaatt
89701 tcctagaacc aattaccctg agtgcctact atatggttca tttggaaaat gaatgatagc
89761 tgcaatatgg taaaatgatt atgagcacag ccttcggaat aagtaaaaca tagctttaat
89821 atctaacttc tgtacttact aactgagatc tgaacaagtt acttaaactt ctaaggttta
89881 gtttctccac ttacgaaatg gagaggaaaa cacataattc atagttcatt gtaaggacta
89941 agtaaaaaaa aatacttaaa tacttagcac agggcctagc aacaaagata tatgaactcg
90001 gtacacacag acatacaaac acgtacccac actgcttaac tttagctaga atatttttaa
90061 aagcattatt agtttgagac tagattcatc caagttggtt tagcattcta ctagggatgt
90121 tacttttcat atctttcttt gtttttgttc ccaaaagcta taaacacaag ttttatcttc
90181 tttcctgaaa caattaaaaa gaaaatacaa gaaaatgttc aaaaaataat ttactagtgt
90241 ttttgtttgt ctggaagggt catcctgcag ctttaatcac agctgaagat cattatttca
90301 aaaaagacac atcttttaga tatttaaata tgtattatca tattatgcag tttaaatgtg
90361 aagtcatcta ccctctggtt tactgagatt ctgttacatt ttaaataaat agtaaatgct
90421 gtagttaaga gtgctatcac tgagaatcta agaagacaca ttagaaaggg attattactc
90481 caagaagaat tctctttcct taatcattac tttttcatta aaatatttct gaagtttata
90541 caatacaatc atagtagtga ccttaggcat atgacacctt agtgctataa aaactctaac
90601 aaatctgttt agagcccaat aatataaatt tctgtgaaga gaaatataaa ccaatggtaa
90661 aatgccatgt tgaagtttct aaatagccca cctagtaatt tacatgttaa gaacatattg
90721 aatatcttgg gaacatgttc ctagaaaaga ttctccatta taagcttttc ctttactgtt
90781 aatgattgct ttgataaaat gacccacgaa aaaagacaat tttctccctg tgttatctgt
90841 gtctgaaata ttcttcactg attttacata tgctgataat atgaacaagg taatttgtct
90901 gactctggtg gggcaagatt ttcagtaaga aatgcaaaat tcacacatag ctcttctgta
90961 agaagtctWt gcagtgtttc agtttatata aaaagattta tattgcctga ggcatctatt
91021 tttttttcct cgaggcaagc tgaagtggca caaatgaaat tgtttttcac actgttaatg
91081 tattcctttc tctttcaatt tctcagagag ccgacatagg gatttctgct ttaaccatca
91141 ctccagatcg tgaaaatgtg gtggacttta cgacacgtta catggactac tcagtggggg
91201 tactacttcg aagggctgaa aagacagtgg atatgtttgc ctgtcttgca ccatttgatc
91261 tctctctatg ggcttgcatt gctggcacag tccttctggt gggtctactg gtctacctct
91321 tgaactggct taatccccca cgattacaaa tgggatcaat gacgtctact actctctaca
91381 actccatgtg gtttgtgtat ggatcttttg tacaacaagg taaggagcaa aagtacattc
91441 tagtatttaa aaaaaataga atgtgatgtt tccaaagcca tgtattcagt taataaggtt
91501 ctgtatctga catcaataag tgttctaaag ggtactcaca gaaatgctca gaaaagaaca
91561 tttgctactt cctcctagtg gatctcagac acaccaagtt tccagcaata atggttcatt
91621 gtcttttgat gtaagtttaa tttcatcaac aaaggcaaat gtgaatgtca aaggtaaagc
91681 tcacagaaaa catcaagatc agattttcct ctccaaactc tgactttctg agaaatagcc
91741 ttataaggat gaaattactt attaccagag aaggtactat ttaattattt acatatgtaa
91801 gaaatgttag gacttgttct ttgttcagag acattatttc atgcattaaa aatattgtca
91861 ttgaaaagac tttggttaat ttgagactga tgagaaattt tgtgttttat cttttacttt
91921 gatatgtaat ctttactggt gatttaactt acaatttcac tattttctaa agaatgtcat
91981 tgctctgtta tttaatatcc aatctgccca actctttaaa cttgtccaat taattatttg
92041 gctctcactg tttcaaaata gaataatatt tattgcattc cttacttgag actattgcct
92101 catagagaat gaaatagtaa gaaaattgat agtctcttgc aagctctaca catatattga
92161 agaggaggat gtttgctaca atggagacat tatacttttg tgctaatatt tacaactctc
92221 aataacatct ttctttttat tcctattttc tcttggacct tccatttaac ttcatttttc
92281 tattctaatt taatcaaaga ttagtctaga accttcatct gacttgctaa cttctaatct
92341 agcccttaaa ccttttgtaa gcatgctttt ccctccaccc ttccatatcc agggctctaa
92401 gcttacctgt tactgtatat ttttcacaga cacttttagt tctttcatta ataattaatt
92461 cagtaaatat ttaatccaca ctatctaagt agaactttcc taagcaatta gtgtatgatg
92521 gtgagcaaga aggtctttgc catcattgcg ccacaacaga agtctaatga gacacaaaca
92581 tgtaaacaca ggattacaat tgtgggtaga aagttctgtg gtaggataag catagactgt
92641 taggagatca taggtgtaga agcacttaat atatgttaat gtcgcagaga aggcttttta
92701 aagtaacact ttatacagag agacagagtt aactatggaa agtgcttctt gaggaagatg
92761 aatggagaaa aggagacata aacggttagg atggaatttc agaccaagaa gtgagagaga
92821 aattaagata gtccaagcag agaggatcta gtaaagatga ggctgaagct acatttgtta
92881 ttataaggag tttgaactga tccttgaaat gatcagatat ttaaagataa taaatgggaa
92941 aatacagatt caaattttac tctagaaata ttctggttat aatgtggaga atagattgaa
93001 aaaaggcaat gtgaaaagtc agacaaatta gaagactatt acaataactg agcaaagagt
93061 tgatgtggct tgctcaaagg taaggtagct ttgggagtca agaggagagt attgatgaaa
93121 tggaatcaac aggctttgat tacctatagc gtggtgtggg ggtcagtact gaagaaaaaa
93181 ggagataaag ttttatccta ggtttttgaa tagaaaatta tgtatattgg atgtggatct
93241 catttactga aaaaataaaa cagaaaaaac agatatgaca ggagagatga tacattcatt
93301 ctgctgcaca ttgagtttga gctatccgtg aaacatccaa aagagtatta ctaatgtgtg
93361 cttagtggtt tgatatattt gtcagatgca cagaagagac atctggactt aatatatatt
93421 taggagatat tggaatgtag gaaattttga agacatggga gttaatgaga tcacacagag
93481 tagttgctct taattgcaaa ctcctgattg aggaccactg gcccatacaa agcctaggac
93541 agagctctaa ggaaaggaat ggatagagga gcactcagtg gtgcctgata aggagtgact
93601 ataaatgcac aagtgaaaat cagagcagct agtagtatac gtttggttga ctgtgttaaa
93661 cctttcaaag aatcaaatga aattaaggct tgaaaggttt ttgttggatt taacaataaa
93721 tttattattg aagcaaaaaa ggcttgtctt tgccctatga tgtctgaggt ttcagctaga
93781 caattcagag gccagaggct aaaatcatcc aaaggcttgt tcatttcact cacatgtctg
93841 gtggttgatg cggtctgtgg gctagtggcc tcagcttttt ctccatttgg gcctctccat
93901 gaggtcactt tgcatgggat agtttgactt tctcacagca tggtagttgc tttctccaca
93961 gagaacatcc caattaaaga attttatgtt ccaggttggg agtcataaac ttgtgtgatg
94021 gtgtgaattc ctcttgcaga gaacatcctg gatgtaccca cctcatgggt ttttatgaga
94081 atttattaat gaatggttaa aattgtgaag ttcttagata taacttatat aagtgtcaca
94141 taagtgttta ctaaataaat aaaacaatga attagactca gcggcagata attgacatat
94201 gtaaaaaaag ggataacaag acaaatgatt gtagatcatt ttgatgtaSa tattgattga
94261 aatagtagat catggggtat atggagaact agaaagagaa ggaccaagaa tgcgaatgta
94321 aagaagagta agggtagaaa ttgatagagt aggcctggca tggtggcaca tgccagcact
94381 ttgggaggcc gaggcaggcg gatcacctga ggtcaggagt tcgagaccag cccggccaac
94441 atggtgaaac accatctctg ctaaaaatac aaaaaaatgc caggcatggt ggtgggcgcc
94501 tgtaatccca gctactcagg aggctgaggc tggagaattg cttgaaccca ggagcgggag
94561 gttgcagtga gccgagattg tgccattgca ctccagccgg gatgacaaga gtgaaactcc
94621 atctcaaaaa aaaaaaaaaa aaaaaaaaaa aagatagagt agttaggaga gagtagtaga
94681 ggtctcaata agattacaga aaaagagtga aaacataatt ataaatgagt ttggagtctg
94741 tatttataaa ataggacatc taagcctaat tttttttgaa atgtaataga ggagaacatg
94801 ttgtaggtat gtggatacaa attccagatg ttaagatttg gtgaaggact tcaaagctga
94861 tcaaggaact gagagataaa gggttaggtg agccagctgt aatgatggct tggattagac
94921 tggagaaaaa gaaattgagc catgtgtcaa agttgtgaat aaataaggaa ggatgacaag
94981 gatgttgata gatatgagca ttgaccagaa ataggaggtg acatacctag atataattaa
95041 cctctgtttt catcttactt cttccaattt attcagtttt tcagtgtatg atttcaacaa
95101 ctgccaatat gctgatgact cccagatcta catcatctat tcttgaactt cagatttgta
95161 tattcaactg ttacctaggc aactctctga ctataccaga ggcaccataa atttgaacat
95221 gccaaaaatg taattcattt tagaatttga accttcttag cctctcacat tctctattcc
95281 agctgatcca ccaccaacca ttcagactca tgttagaaac tctgaaaata gttttcattt
95341 cttcctcttt ctcatcttgt atatccaacc ataatcaagt cctgtcaatt ttacctacta
95401 aatatttcta gaagtggaaa tctcttctta tctagaaaga ttagcctaga ttgtcaccta
95461 gattgaggct acctcatttt tcacctgaac tataggagtc tcataagtct ttcttcttcc
95521 agtgttgtct cctgcaaata tacgattcac atacttgcat atatacccaa gttatccatg
95581 ccatggaaag ataaacacat aattcccatt cttcagttat tttcttggct tcccaatgcc
95641 cacagtttag aagtctgagt tcttcagagc ataaacaata ttccatcacc tggctcctgt
95701 ccaacactca gttctcatct cttaccattc gtgcctcaca tttaattgcc cagttactcc
95761 aaacttacgg tttgaccata agacaagact ctttcctaac tcttcacctt Kgtatctgct
95821 ctttccaatt ccttgagtgc ctttgttacc aacctgatta gactctactc ccactctctg
95881 tctttataca gcaagttcct attcatcctc taattttttg cttttatttc tctactttca
95941 agaagtcttt cctgggattg agaaccatct tctatactcc tcaacatgta tttgtcatag
96001 tacttaccaa ccatagtgta attccttttt ttaaacatgt ctccacctga gtcctttgaa
96061 tataagatag tgtactattt aatctgtgta gtctctttgc ttatagttaa gtgccttgta
96121 catgttaggc atttaataaa ttcatattga taaatttaat gaatggatgc taaatactta
96181 actaaataaa gcatggaggt tagctgcaat attttgttta ttagattata gtctcaaata
96241 aaacactaaa attatccaca aatgacttaa aactcactaa accaaaaaga aatttatctg
96301 taatgtcccc actgagttgt tgtaagaaac taagaggagt ttataagtaa ggcatcctct
96361 tggttgaagc ctcatttgtg aagacgttga catacttatg aattggcttt ctgaattctt
96421 cctccaagtg accaaatttc aataagtttt ggagcacacc caggaaaaaa gataaataga
96481 attacccata tggttagcat gcttttctag ttctataaac atgcaacaga taacaaaagt
96541 tataaatatg ttaaatacct ttctatccca ctgctccctt aaatgagttg cacagcctgc
96601 aggttgattg tacatttata caaatttata atgacagata atgctgaatc gcatatgatt
96661 agctacttca tctttgaaaa gctgctattM ttccacttgc taacaatgaa aaggtgaatt
96721 cattaaaaga Yactgagagc atctttatgc tctgattaaa aataaatgaa gaaaaggctt
96781 ataaacccta tgaaaaagag aataaatcaa tgacagtgtc catgtatagg caattgactt
96841 ggaactggtg atatttccaa taaaaataca gtcttttaag aggaagtaca gctgttattt
96901 cagaattgca gtcttcattg agccactata ccagattgaa tagggataaa gaggcatttc
96961 atgtgttatc agacagattt ctaaaaaaag agttcacgga cagccaaaag cagaaaaatg
97021 ttaaatatat gtgagttgtt tactaatcct gacattttaa aaaatctgta acttcaaagc
97081 attctggaaa aatagctcag ggaatccaac tgtgcaaaag cagtatggtc cattatagag
97141 ataaactcag tacatctgtt tggctggaac attaagagct tgtgaattag gttgtaaaat
97201 gcacgaatga gagaccaatt ttatacaact tttgttgtca ttttatgtcc tccgttatct
97261 ccatgtgtac atacatctcc tttcaattaa tattcttaag tgaaactgga tttgattttt
97321 agaagtctct tatgtttagt ttattggcag tgactctatc atctggtctc cagaggactt
97381 caatgacttt gcaaatgtag ttgtatgtaa tttKctttct gtttacatgc aaagggaaat
97441 ttcagaatta tcttcagatg tttgtgtctg attatgggaa atagaaatat ttatagtcat
97501 gtaaaatatt tgaagcaacc acatatatgt ttatttagca gcaagcattt attaagcacc
97561 aacaatgtgc caggaacatg agtagcaaaa ggaaaataag gaataactct taactagggt
97621 ttaataaata tttcacgagt atactatgtg

Following is a genomic nucleotide sequence of a PDE4D region (SEQ D NO: 4).

>5:58309301-58408050
1     ctctaaatat tttccattga tgaatttcta attgtttttg gcctaagtag tctgaaaaca
61    atacacagat ttagggttgc aactaaaaaa acgggaggat ttctaagtga atgcttttga
121   atcctttgtt ctcattatac atcagaatgt ctgtgtacag cagttaaggc ataaaggaaa
181   gtaaaaatgc agttgaatct caatactgta ttttgctttt cagaactgca attcttcaat
241   ttacattaSg catttaagat tgttcaggaa gcaaactaaa ataataaaac aaaatgtcct
301   taaatgtaat ctcctttact tctacatcac gccagtgtgc tactgggtat attatatatc
361   ctcccaatat atatttaagg aggtggtatg ggtccaatag taagaaaatt ttcttatttt
421   ttcctgacaa tattctgcta aaaatcattt gaatatgctg cactgtgatg cattacaagt
481   ttggtagata tcagtattca aagttattta aacaaatact ccattgatgg tataaaaata
541   aaWccttcaa aatgcataag accaaatata tctatatttg ctatttttac atgtttttaa
601   aggtacagta ttaaacataa agctgtccag gaaaaatttt cctcaaaaag aaacgtacta
661   aaattctata tgtagattct tggcaatatt gttcataatc atagtacaaa tggttcataa
721   tcacagtaca aatgtgaagc ccttcaaaaa taacatcagt tgtgagctgg ctaaaagcta
781   ggagctgaat tctggcggat aatgagggct tcttatattt actctctata cttttctgca
841   tttctgaaga tttttagcca agaaatggct aacaaaattc aaataatgga agtctatata
901   ttttaaaaac atttttaaaa atctgaatca tccacagatt taaagggagg taaggaatca
961   tgactacttg gaYaaccaag actgaaataa gttttctaat ttttttagaa ttcaagtaaa
1021  tctctaaaac aaaggggaac ttgtccttca aaaagaaaga aacaaagcaa acgacRaaca
1081  ctccaatctg aaaaatcctc agagtaacag aaaatgaatc ttttaaaatg tgttgtgatt
1141  ggctcccagg ttcaagcgag tggctcaaat accaaaaatc tgtgaagtga tgtttcattt
1201  aggactgaat aaggtttttc tacctccttc tattgtggag acaggatcac aatgctaatg
1261  ttaccacagg gcatgSaaaa agcctgccaa gcatggacac aatcttattg aattgtttat
1321  taattcattt cttgagattc acaaaacagt gtaataaaaa ctttgttcag ttttgcaagt
1381  ataatagaca atcaagtttt cactttagta tcacttacat agttaagtaa tgtcaatttt
1441  taatgtcacc tagggcttta tagctaaagg tgaaaaaaac gtattcctcg tcaggttaaa
1501  gtagtataga tttttagaaa cagccacaaa ttaattttat aaactttatg tgcaacatac
1561  taactggaat ccataaatca gtataagtaa tttggcccRa taatttattt gcaatgaatg
1621  tatttaatta ccatatattt ttcaagcaca attcaaacat ataccacatg acaaacagaa
1681  gaactactgc gtatctaaaa aagctatttt aacactcaaa ctacaactga tgaaagttat
1741  acaatcttat cttgaccact attaacacgt atttcttcct agttcatgtg taaggaaaga
1801  atacataact gatagtttga acaaattaca gtagagtcta agctttctaa ataattttgt
1861  aatcagagta aggagttttc aaaagctaag taagtcttac ctttatgtag ccccaagaca
1921  ctgacagtaa atagtttgct tcaggcattt ttgattgatt atatgtatat atatatatat
1981  gtatatatat agtaagctct aaaatgtatt tagcttcaaa atctcgacac tttaacaacc
2041  tccacaagcc acgcagagta tgagttttta aagatttttg gtaattaagt atacataaga
2101  aagcctcgca ttaaatccca tccagccgtc ttcccatcga atacatgcca tttgtaagac
2161  tccagaatgg cacaaaattc agcaactatt ctgaataaag aaaatccacg tgataaagga
2221  atatctaaag atctaagggt ctgcatcttt ctctcccgag ctccagggct taatgaataa
2281  tgtatgtcaa gatgcttagt tgcaagaaag gctagtcctg tcaaaagcta ccaaataagg
2341  aactgcaggg aagccgggaa gagtgacaag aagcccctcc cataaaaccc caactctgca
2401  ccaattgcat tccagctctt agcctataag gcgagggggt gtggcctcgg tgcaggctgc
2461  tttgggattg gttggcaagg gccagcctct ggggaggctt tctcctcacc cgacaaacgc
2521  cattgaataa tggtatccct gcagctctga ggccaccctg agaaaagatg aacggggctt
2581  ccctcttgca gtgatgaata atgaccataa aactcctcaa aattcgtagt tcattcttaa
2641  gaattgccca tccttttgga gggcattttc tatgaatttt aatgtcctct ggcttccctt
2701  ccttccaaat aaaaactgct ccaaaaatgg caatcgagca ttttaattgc ttccYtctct
2761  caaatccaaa acaagtggct ccctggtgat gtagaaatga ctgtagttct cccctcatcc
2821  aacataaaag catagggatt aaacaatacg actaatgtcc agaattcaca gatggcacag
2881  atggcaatga cacattccct cacctgtgtg Scatggctgc tctcagctcc ttttgtttct
2941  attgttatgt atgacagagt tctaaaacct tcaaaatcta ggggtgggac cacttgcaat
3001  tctgggggga gggaaatgtg aaagctaggg cactgtgtat gcataaggaa tgcagtgttt
3061  tgtatataga actcaggatc tgtattttga tcagaagaga atattttatt tatttattta
3121  gagacagagt ctcgctgtcg cccaggctag agtgcagtag tgcgatcttg gctcactgtc
3181  acctctgcct cccgggttca agcgattctc ctgcctcagc ctcccgagaa gctgggatta
3241  caggcgccca ccaccacgcc tggctaattt ttgtattttt agcagagacg ggatttcacc
3301  atgttggcca ggctggtctc aaactcctga cctcagggga tctgcccacc tcagcctccc
3361  aaagtgctgg gattacaggt gtgagccaac gtgcccagcc agaacagaac attttaataa
3421  ctgtacacaa agttaagaaa ggaatgagtg gctcttagga atccagaatt gagctcaaga
3481  gtaatcagac attttacgag gagggttaaa tgagaaatca aagttctttc tagaaaaaca
3541  gcattttcta tttggtttgg tgtagtatta ccctgtaact ttaatactaa agaggtcttg
3601  tgggtgagaa acaaggaaaa actaggtttt agttgatttt agcatataat tctgtgcatt
3661  ttacctcctc tccatctcaa agcctgtgct tactactctg tgctttaact tgctggtatg
3721  gctactctgc agtcttcctg acatttaact atttccacat gtggcatgtc tgtcttagag
3781  agcatgctct ctgataagca ctcctgtaac actgtgtctc tctgagtttc tattggcctt
3841  tagctgctac agtcctctgc ttttgttaac atctgttgta ttcatttcca tcactcttac
3901  tcctctacaa aaggaagaaa ttaaagctcc aacattccac taaagctacc cccagaggaa
3961  tactaaggtc ctccttttct ctggtctccc tacttctctg aagtgttcca catgtttgat
4021  cattcctact actctccctc aactcccagg atcctgaact tctttaattt tccctaggcc
4081  cacaatcttc aatgaccact tctaacttga acttcagtgc catatttctg aatacaagat
4141  tctcctctcg aatgccctgc tagtcacgac aaactttttc aaaactgtga gacatttttc
4201  tatttaaagt agttcttagt ataattactt ccatttcagt cagtgagcct atatttcttc
4261  cagtatctca gatggaatct atgactcttt cctaccattc aacaggtgtt tcatgtgcgt
4321  cagacactgt caggtgttta atataaacca tctagctgaa tccttgagta acccggcatg
4381  ttcccctata cggcatcacc ttccctcctt cattcaactt tgttagcaag ccctatcttt
4441  tatccagagc tgtttctttc agtttRttcc ttaccattac ccctcccgta atctgaatcc
4501  aaatatttaa aagactgaga gaaatagaaa gtaactttaa tatcaccccc tctaactccc
4561  tcatttccac ataaagttga gacctagaga gaaaaaatga ttttcccagg ttcactctgg
4621  tactgagaac aagaattgca agtttaaatc ttttgtgcac aatcactgac aaataatctt
4681  tttagtgtgt cagcttcctc atctgtaaag tggattactg tggcagctag tctccaaaga
4741  tactcttaat gagccacact gtcctacact gaatctgagt tggcttgtgt aaccaacaag
4801  tgatggtgca tgactgctga ggctaagtca taagaaatct tgcagctctc atcctgcttt
4861  ctttgaatgt gctattggga cccctaagcc attaggttga aaaaaaaaaa ggtctgacta
4921  ctctgagagg ctatgctaca agaagtccat gttaacttca cagacagtga agagagaaag
4981  cgcaagagag cacttgcaca agaaagagca cacatgcctg gccagtcttc agcaatgcca
5041  gccaccccag cccagagcca gacctgaggg gcaagaagcc atcctagcca tccagccagt
5101  tcagctttca gatagctacc cactgggctg caaccatatg aggaatgcca agcaagaatg
5161  tctagccaag accagttaac ccacaaagcc atgaaaacta ataaaaatac attcatattt
5221  caaagtatta ggtttgaagt gatttcttac atagcaataa ataaccagaa cagatacata
5281  tgaggattaa atgagaccat atatgcaaag cacttaaaac aacaaatggt acaaagttga
5341  agataagtag gtatcagaca gtaaagatga cagtgacaat gatcattatg ataatcattc
5401  tattatacta atatttaaaa aaattctctg gtttgtcatt tgtttttaaa atatatatgt
5461  tatatttcct ttcaattgca tcacagcact ccatgtctca gggccaaaat gctgtaattg
5521  cttcttagct catcttcttg cccctggctt ttccccttct agtctcatat tgacctctac
5581  tgccagatca attttcctca aatccagccg cagcctgtta cttcaaagct agtaatgcct
5641  gcttcatcac atccctctgt aaattccaga ctgccatatc tgaccttaat ccccaacgcc
5701  aagcctattt tcagctgctc tattacattt gctctgttcc tgaaagacta gtaYtcctcc
5761  aYcttcacca tacagctgac aatcccatct ctgtcttacc aaattctcgg cctaaaatac
5821  cctccttctt ctactctgcc aacctcaaac tggttttctc agtcagattt aaatcattca
5881  aagtacatct taggaccaaa tattccagcc tttttccaaa ttctaacgat tctacatctg
5941  ccccttcatt caatatttat taagctYcta cYctatgtca ggcactgcct gagaaagaga
6001  aacaaagctt tctcacctgc agcttatatt ccaaaactaa gttaataaat acataatacg
6061  tcatggggtg ataagcacaa tggagagaaa gaagggataa ggattacgga tagaggttgc
6121  tataaggtga tcatggaaag cccctctgat gagatgacgt ataccaggag atctgaagaa
6181  aggaaacaag ccatgtggat tgcggggtga gaggtagggt agagaaggga aaaccaagtg
6241  aggaatggca aaagcaaagg cctcgaggca aaacatactt ggtgaggctc cctgaagaac
6301  aggtggaagt gtcataccac ttgtcattta aatgcaaatt acactctgca gtcaggctca
6361  ctgccttccc cctcctttaa aaaaaaaaaN atccatccat ccatcaattt tattctttga
6421  caaacttaag actcagttac tttttacttt ttataacaag tattcatcct gaaatgaaat
6481  tcctaaatac acttccttca taatactgta ctgaatacat ataggctaaa tactccctac
6541  atagatttaa aaaaaaaatc agtataatgg cttatcagtg aaataaagga gaaatgagta
6601  atttctatca aatactacaa aatataaata cttgtacaca actacatttt aaaaagtagt
6661  caaaaactac tataactgat tagctgtgtg accttgggga agctatttaa atttcccata
6721  cttcagttta ctcacatgga aaatgagaat aatagtttct accttacagg gttgttgtga
6781  ggattaaatt agtgcatata cataaggctc ttagaacagt aagtgctatc taagggtttg
6841  ctatgatttc tcttatgggg agaattacca caaaagtaac agtagcatga aaatgaatac
6901  aggggtgtta tgttagaaaa attttccttt ttcctgatat gatgaatgat tctttgaaag
6961  gtttcaaatg taacaaagtg ccaYcttcac aatatacact attgctgcat tactagaaaa
7021  tttggtgtgg attaaaacca tgtaaaaata tttaatactt atacgtaaaa agtagttggg
7081  ttctaggctc agagaattat aaatgcattt tgttttttca cctatgtgaa tagctgctgg
7141  gactttttgg aagttgtgtg ggacacacag caaacctccc tggtgaaagg ctgtcccatg
7201  tatcccagga catctagcct ccctggcctc tgcYcaccaa gcaccaggag tactccccag
7261  tcactgtccc ctgaaatttc ctgaatgttc cccagtgtta agaatcactg ttctattttt
7321  ttcaactgtc ttcaactttg actcctcatc cagggtggag gatctttgag gccagaaaag
7381  tatcttatta tgtccctgta tttctcaagg tagtgtacta ttgccacaaa aagattgttg
7441  gctgatgcag atgcaaaaca tcaatatttt tatactgatt ataggtgaca ggaaaggaca
7501  agggttttca ccctcagcaa aatcatcaaa aacatttaaa acagattaca tatagtagat
7561  aatactcctt gtcttaaaac attatgcaga aacacacata cagtttccct tccctctaaa
7621  atctgcagcc tcttctccct gctgaatgtg agtgatggtc ccttcattca acagacttct
7681  atttggcaac tattatgtgc caggcattct aataggtgct ggatgtgaaa ggatttaact
7741  tattcaaata agtccctaat ttgctgcaat aagggagatt atttaatcat attgagtaaa
7801  aagtaccaag gaaagtttcc atatttctag ggaataagaa atcttttcaa aaatctctga
7861  gccaaattaa aattcatttc attttcccta atctccattt ctaggagact agaatctcct
7921  tcctattgcc acggtagctg ctatctgcag agagaatcta gataatttag aagagaaatt
7981  tgaatagagc cagagagata caattcttat agtcagactt ggcttcacca aatttaatga
8041  agaaacttta ataagttaca acatttacta ggcacctact atgcacaaag aactttacat
8101  acctcatttc taatcttggc aacattctga taagataaat attatcccta ttttaacatg
8161  aaaagatgga ccttcttaca tattcaataa cttgcKccat aggtgacagg attgaaatgc
8221  aaacccagtc cttgctctta atggtgtatg acagctcatc atataattag gctgatgcaa
8281  actctatgat aaacagtatg aatctaggag tggatttgtt tatcctcaat ctttttgcta
8341  actttacagt gccttgaatt aaagaatcRt ggttactatc accatggtca gatctctgga
8401  cagggtgagg aagcaggtgc ccacaggcag acagccctgc catatagtga gacacgctta
8461  tgtttgggag cgctatgtga acaacacaca gctgccctca tatgcagatg taatgatgac
8521  gacacattca gacaaggaac tgaggtccag aaaagttctg acaacacaac ttgagggaca
8581  gcgttacagc tagagctcag gtttcttatc tgtaatccac tgctctcaaa tgcccaaatg
8641  attttatata catatatgca agtgtgtttg ttgaaataga aaaaatagtt tcttggcctg
8701  gtgaagtgcc tcatgcctgt aatcccaaca ttttgggagg ccaatgcggg aggattgctt
8761  gaggccagga ggtcgagacc agcctgggca acatagagaa accaccgtct ctacaaaaaa
8821  tttaaaaatt agccaggtgt ggtggcatgt gcttgtagtt ccagatacta ctgaggtggg
8881  aggtgggagg tgctgaggtg ggaggatggc ttgaggccag gagtttaagg ctgcagtgag
8941  ctatgattgc accactgtac tccagcctgg atgacagagc aagaccttgt ctcaaaaaag
9001  aaaaaaaaaa ttcccataat gttcagttag ttctcttttt cagacttttc ctagaaatac
9061  ccctcagaat ttctagaggc cacttccctg ctattccttg ttttctcagt ccttaccaga
9121  tacccaagca attaacaatc tttttgaact atttcagtac atttcaggat atttgaaatt
9181  ctgctgatgg agctgaactg ctgatacatt aacacatgct gtcattattg actttgtaac
9241  agaggtcaag agagaaaaga tgcatttttg aatagggaaa aacaaaacta aattatgggg
9301  aaactaaggc tcagagaggt caagttgtct tgctcaaggt cacaaagcca gtagtaagta
9361  acagggccag gatttgaacc cagactgtct ggtttcagag tactctaaat tgccaaaaaa
9421  agaaaactat ttcaggcatt attttatatt tagcctagag cctacataat agatccgtta
9481  atataatcaa ttctaagctg tatattatat tactgtgttt gaaatattta agaaatattg
9541  tttaaaataa gtgtattagc tcttRgacct ctccaacata aggagttttc caggcagtag
9601  cactgtttgc agtaggactt acattattgg aaaatatttt atcctataat caatctacat
9661  ttattttaca cctttcacct taaaattaaa agtcgtggta gttattaatc aagaagcaaa
9721  tctatataac gtggtctctc atatatccag tactgttcag cttgaacaaa tgcaaatcta
9781  atttttaaat gtcccttgaa gaattctgag gcaaagttaa acttcaagta ttaaacagaa
9841  tagccacaga aaactagtgt ttctcagcct ccctttgctt tcaaatgcct taaaaatatt
9901  ttagaggcag acaataatct agaattgatt cataaccaat attttacata acagaatgtt
9961  acatgtattt tcatattata aaaactatac tttctttgat taatgctagt attctgaaaa
10021 catcgttagt gccagtaaat ccactaaatc tgtttgtatt tactgccctc tagtgataat
10081 ggctctacat ttgattaaga aaaaactatt cctttcagta aatgaaaaca tctcttgtaa
10141 gttttatgga gatttgagat taagaggaac tccaggctgc ttttttaaaa ccaaataatc
10201 atctgaacaa gctgagaatc gttctctaaa tgatcttatt ttaatttttt tttaacaaaa
10261 aagtctcatt taaaatagta aacacctgca acatgtatgg agaaaggaaa ccatttttgc
10321 taagaggctc cttgaagctg aaataacatg agactgatct gatagctgta tttatatttc
10381 tcttctacat gggctaactc tcttattaga tgttagcata gtattctgta attgttgata
10441 cagtttgaat tcatatatgg tagagacaga gttttgtata actatttagt caatttacct
10501 aaaaccagca gcagcagcat atctactaca gacttttgtt tcactgaaga ataatcatga
10561 caataaatgg gtgcaaaaac ttcacataaa tgaagcctcc acaaaaataa tcttgtatga
10621 aaaagtattg tctccttata gagaaaatta ggatctccaa ttcacttaaa ttggcctaaa
10681 tgaataccat aatcatcata aaatagttac taatgtcccc tctgtacttc gtggtatgct
10741 ttgtggaaac aatttaaaca aagagaatat ctgtctccta aagtaataat aactagtgta
10801 aataggcatg cttccagaaa tatataatct tttacacata tcactttaat atgtgcatct
10861 taaaataatt ttaagtaata aaatttgagc tataaaagag aaatgatatt cttttcaaca
10921 aagaatgctt gaacaattga gcatccactc ataaaacaag aaacttcaac ctttatcctg
10981 caccacacta acatctactt gaaatgaatc atagaactaa atgtaaaatc taaaactaaa
11041 gaacttctag aagaaaacac agtagaaagt agttgtgatc ttgYgttagg caaagatttc
11101 ttaaaccaca aaagcatgaa gcataaaata aaaaatggta tactggactt taYcaaattt
11161 ctctttgaaa aaaactgtta agaaaaYaga acaacaagcc acagacttga gaagggaata
11221 tgtacaaaat gcatatcaaa taaaggactt gtatccagaa attatttttt aaaaaatctt
11281 tacaacacaR taagaggaca atccagtgaa aagatggtca aaagatttga acaaacaaac
11341 acttcataag aatcaaaaag atcaataagc atgtgaaaag atccaYgacc atcattagac
11401 attagggaaa caaaaagtaa aaccacagtg agatgccaca Ytacatactt actagaatgt
11461 ataaaataca aaatacaaac aatacaaaat gctggcctgg atgcagagca aattaaattc
11521 tcatacattg ctagtgagaa tgcaaaatgg tatagaaact ttggaaaaag gtttgacagt
11581 ttcttataaa gttaaaaagt agagtgacca tatgacccag caatcctact tctaagtatc
11641 tacccatgag aaatgaaaac atatgtctat ataaagacct atatgaaaat gtttatagca
11701 actctctata gcagaaaccc agaaacagtt cagatatcag tcaactggtg aatgggtaaa
11761 caaattgtgt ccataaaatg acatactgga aaaaaatgaa atactaaaaa gcaataaaat
11821 gaaacaaatt accaatagat gcaataacac aaaatgtatt ataagtgaaa cttataattc
11881 aactcaaaac tcaaaagtat tgtaagtgaa agaagccatt cacaaaaggc tatatcttta
11941 atgtattatt cattctggca aggcaaaacc atagggacaa tcagcactgg acgtggggtt
12001 tggggaatga ctataaaaag caagagaaaa ctttttgggt ggtataaata tcctatatta
12061 tatagtacat atatattata attacacatt acctatatat ctagattatg gtggtggtta
12121 tccaattgta cacatttgtg aaaatgcatt caactatacc tctagaaaga gtaactctta
12181 tgtaaattat acctcaaaaa tctaactttg aaaaatatta gctgatgtta tcaaattatt
12241 tcaattccag atttgcttaa ctttacaact ttgcccaaag ataaatgcaa aacaaaaacc
12301 tcaggcatag ttatgtatga taaaacacca tttttaccct ttcaaagagc tccaccatta
12361 tttcccagaa aaYaggcata cagttatcca aggtccacat tagctttaat gatacaacat
12421 tcagtgaatt cccagttgtg aaaagccttt aggttggcag ggcgctgtgg ctcatgcctg
12481 taatcctagc actttaggag gccgaggcag gcagattgcc tgagctcagg agtttgagat
12541 cagcctcggc aatatggtga aaccctgtct ctactaaaaa tacaaaaaat tagcagggtg
12601 tKgtggtgca ctcatgtaat cccagctact cgggaggctg aggcaggaga attgcttgag
12661 cccgggaggc agaagttgca gtgagctgag atcgcgccac tgcactccag ccggggtgac
12721 agagattctg tctccaaaaa taaaataata agagaagcct ttaggtgata aaataatagt
12781 caaagtattc ttttaagtat tattatttta ttttttttta attctttgta ataaaaagtt
12841 ccataaagta aaatattgtc cttttttgtt ttatttttat ttgtatcaat tcaaggggac
12901 aggtgcagtt tgttacctgg acctgatttt taaataaatc aaactcagtt tagttatttc
12961 atggtgatcc tgggagactg tttgtcagtc atcatcgaat agcgaatgag tatcctttgt
13021 atgctcattc taggctgtaa gtaccatgtc agctgatgga gacacaaaaa tccaataaag
13081 taggtatagc tgggggaggt tccaagatgg ccaaatagga acagctccag tctactgctc
13141 ccagcatgag tgatgcagaa gacgggtgat ttctgcattt ccaactgagc tttgaaaaga
13201 gtagtggatc tcccagcatg aagtttgaga tctgagaatg gacacactgc ttcctcaagc
13261 gggtccctga cccccgagta gcctaactgg gaggcacctc ccagtagggg ccaactgaca
13321 cctcatacag ccagatgccc ctctgagatg aagcttccag aggaaggatc aggtagctac
13381 atttgccgtt ctgcaatatt tgcggttcgg cagcctctgc tggtgatacc caggcaaaca
13441 gggtctggag tggacctcca gcaaactcga acagacccac agctgagggt cctgactgtt
13501 agaaggaaaa ctaacaaaca gaaagggcat ccacaccaaa accccatatg tacgtcacca
13561 tcatcaaaga ccaaaggtag ataaaaccac aaagatgggg agaaaccaga gcagaaaagc
13621 tgaaagttct aaaaatcaga gctcctcttc tcctccaaag gaatgcagct cctcaccagc
13681 aacggaacaa agctggacag agaatgactt tgacaagctg agagaagaag gcttcagacg
13741 atgggtaata aacttttccg agStaaagga ggatgttcaa acccatcgca aagaagctaa
13801 aaaccttgaa aaaagattag acaaatggct aactagaata accagcatag agaagacctt
13861 aaatgacctg atggagctga aaaccatggc atgagaacta catgatgcat tcacaagttt
13921 cagtagccaa ttccatcaac tggaagaaag ggtatcagtg attgaagatg aaatgaatga
13981 aatgaatgaa atgaagcaag aagagaagtt tagagaaaaa agagtaaaaa taaatgaaca
14041 aagcctccaa gaaatatggg actatgtgaa aagaccaaat ctacatctga ttggtgtacc
14101 tgaaagtgat gggagaatgg aaccaagttg gaaaacactc tgcaggatat tatccaggag
14161 aaattcccca acctagcaag gcaggccaac attcaaattc cagaaataca gagaatgcca
14221 cgaagatact cctcaagaag agcaactcca agacacataa ttgtcagatt caccaaagtt
14281 gaaatgaagg aaaaaatgtt aagggcagcc agagagaaag gtcgggttac ccacaaaggg
14341 aagcccatca gacttacagc tgatctctca gcagaaactc tataagccag aagagagtgg
14401 gggccaatat tcaacattot taaaggaaag aattttcaac tcagaatttc atacccagcc
14461 aaactaagct tcacaggtga aggagaaata aaatccttta cagacaagca aatgctgaga
14521 ttttgtcacc accaggcctg ccttacaaga gctcctgaag gaagcactaa acatggaaag
14581 gaacaaccag taccagctac tgcaaaaaca tgccaaattg taaagaccat caaggctagg
14641 aagaaactgc atcaactaat gggcaaaata accagctaac atcataaaga caggatcaaa
14701 ttcccacata acaatattaa ccttatatgt aaatgggcta aatgttccaa ttaaaaaaca
14761 cagactagca aattggataa agagtcaaga cccatcagtg cgctgtattc aggagaccca
14821 tctcacatgc agagagacac ataggctcaa aataaaggga tggaggaaga tctaccaagc
14881 aaatagaaaa caaaaaaaag caggggttgc aatcctagtc tctgataaaa cagactttaa
14941 accaacaaag atcagaagag acaaagaagg ccattacaaa atggtaaagg gatcaattca
15001 acaagaagag ctaactatcc taaatatata tgcacccaat acaggagcac ccagattcat
15061 aaagcaagtc cttagagacc tactaagaga cttagacttc tacacaataa taatgggaga
15121 ctttaacacc ccactgtcaa cattagacag atcaacgaga cagaaaatta acaaggatat
15181 ccaggaattg aactcagctc tgcaacaagc ggacctaata gacatctaca gaactctcca
15241 ccccaaatca acagaacata cattcttctc agcaccacat cacagttatt ccaaaactga
15301 ccacatagtt ggaagtaaag cactcctcag caaatgtaat agaacagaaa ttataacaaa
15361 ttttctctca gaccacagtg caaagtagaa gttgggatta agaaactcat tcaaaaccac
15421 tcaactacat ggaaactgaa caacctgctc ctgaatgact actgggtaca taatgcgatg
15481 aaggcagaaa taaagatgtt ctttgaaacc aatgagaaca aagacacaac atactagaat
15541 ctctgtgaca catttaaagc agtgtgtaga gggaaattta tagcactaag tgcccacaag
15601 agaaagcagg aaagatctaa aattgacacc ctaacatcac aattaaacga actagaaaag
15661 caagagcaaa tacattcaaa agctagcaga aggcaagaaa taactaagat cagagcagaa
15721 ctgaaggaga tagagacaca aaaaaacctt caaaaaaatc aatgagtcca ggagctggtt
15781 ttttgaaaag atcaacaaaa ttgatagacc actagcaaga ctaataaaga agaaatgaga
15841 gaagaatcaa atagatgcaa taaaaaatga taaaggggat accaccacca atcccccaga
15901 gatacaaact accatcagag aataacacct ctacacaaat aaactagaaa atctagaaga
15961 aatggataaa ttcctggaca catgcaccct cccaagacta aatcaggaag aagttgaatc
16021 cctgaataga ccaataacag gttctgaaat taaggcaata attaagagcc taccaaccaa
16081 aaaaagtcca ggaccagaca gattcacagc tgaattctac cagaggtaca aagaggagtt
16141 ggtaacattc cttctgaaac tattccaatc aatagaaaaa gagggaatcc tccctaactc
16201 atttgatgag gccagcatca tcctgatacc aatgcctggc ggagacacaa caaaaaaaag
16261 agaattttag accaacatcc atgatgaaca tcgatgcaaa aatccttaat aaaatactgg
16321 caaaccaaat ccagcagcac atcaaaaagc ttgtccaccc taatcaagtg ggcttcatcc
16381 ctgggatgca aggctgcttc aacatatgca aatcaataaa cgtaatccat catataaaca
16441 gaaccaaaga caaaaaccac atgattatct caatagatgc agaaaaggcc tttgacaaaa
16501 ttcaacagga cttcatgcta aaaactctca ataaattcgg tattgatggg atgtatctca
16561 aaatagtaag agctatttat gacaaaccca cagccaatat catactgaat gggcaaaaac
16621 tggaagcatt ccctttgaac actggcacaa gacagggatg ccttctctca ccgctcctat
16681 tcaacatagt gttgaaagtt ctggccaggg caatcaggca ggagaaagaa acaaagggta
16741 ttcaattagg aaaagaggaa gtcaaattgt ccctgtttgc agatgacatg attatatatt
16801 tagaaaaccc catcatctca gcccaaaatc tccttaagct gataaacaac ttcagcaaag
16861 tctcaagata caaaaccaat gtgcaaaaat cacaagcatt cctatacacc aataacagac
16921 aaacagccaa atcatgagtg aacacccatt cacaattact tcaaagagaa taaaatacca
16981 aggaatccaa cttacaaggg atgtgaagga ccttttcagg gagaattaca aaccactgct
17041 caacaaaata aaagaggaca caaagcttat agataggaag aatcaatatc atgaaaatgg
17101 ccatactgcc caaggtaatt tatagattca atgccatccc catcaatcta ccaatgactt
17161 tcttcacaga attggaaaaa ctactttaaa gttcatatgg aaccaaaaaa gagcccgcat
17221 tgccaagaca atcctaagca aaaagaacaa agctggaggc atcatgctac ctgacttcaa
17281 actatactac aaggctacag taaccaaaat agcatggtac tggtacaaga acagagatat
17341 aggccaatgg aacagaatag agccctcaga aataatacca catgtctaca accatctgat
17401 ctttgacaaa cctgacaaaa acaagaaatg gggaaaggat tccctattta ataaatggtg
17461 ctgggaaaac tggctagcca tatgtagaaa gctgaaactg gatcccttcc ttacacttta
17521 tacaaaaatt aattcaagac ggattaaaga cttacatgtt agtcctaaaa ccataaaaac
17581 cctagaagaa aacctaagca ataccattca ggacataggc atgggcaagg acttcatgtc
17641 tgaaacacca aaagcaatgg caacaagagc caaaattgac aaatgggatc taattaaact
17701 aaagagcttc tgcacagcaa aagaaactac catcagagtg aacaggcaac ctacagaatg
17761 ggagaaaatt tttgctatct atccatctga caaagggcta atatccagaa tctacaaaga
17821 acttaaacaa atttacaaga aaaaatcaaa caaccccatc aaaaagtggg tgaagtatat
17881 gaacagacac ttctcaaaag aagacattta tgcagccaac agacacgtga aaaaatgctc
17941 atcatgactg gccatcagag aaatgcaaat caaaaccaca atgagatacc atctcacacc
18001 agttagaatg gcaatcatta aaaagtcagg aaacaacagg tgctggagag gatgtgaaga
18061 aataggaaca cttttacact gttggtggga ctgtaaagta gttcaaccat tgtggaagac
18121 agtgtggcaa ttcctcaaag gtctagaact agaaatacca tttgacccag ccatcccatt
18181 actggatata tatccaaagg attataaatc atgctgctat aaagacacat tcacatgtat
18241 gtttactgca gcactattca ggatagcaaa gacttggaac caacccaaat gtccaccaat
18301 gatagactgg attaagaaaa tgtggcacat acacaccatg gaatactatg cagccataaa
18361 aaaggatgag ttcatcatgt cctttgtagg gacatggatg aaactggaag ccatcattct
18421 gagcaaactg ttgcaaggac agaaaaccaa acaccgcatg ttctcactca taggtgggaa
18481 ttgaacaatg agaacacttg gacacaggat gggggaacat cacacactgg ggcctgttgt
18541 ggggtgaggg gagtgggagg gatagcatta ggagatatac ctaatgtaaa tgacgagtta
18601 atgggtgcag cacaccaaca tggcacatgt atacatatgt aacaaacctg cacattgtgc
18661 acatgtaccc tagaacttaa agtgtaataa aaaaatWaaa aaagtagata gtcccaaccg
18721 ctgtattctt tttgaatcct tttgccactg tttcatatca cagttttttt ttttttctga
18781 gccctagatt atcagtctgt tctttttttt tttttttttt tttaatgtag tccataggaa
18841 tctgctgcct cacRgtcagt atcgtgcccc ttaccccaat tctaatatga caggtttttg
18901 tctaagacag catttcctag aatgtgttgt aatgtacagc agcttcatgt gataattctc
18961 aaagactccc actgtcaaat gttagcatat tcaaggctct gccaggacct gtaatacata
19021 ttctttcttt attctgttta acttagcatt tctgaaacct atcttactac tgaactcttg
19081 acatttatta acaaacaaat acaaacRaaa ataccagggt tccaagtaaa attettatat
19141 aaagcaaagg gctttaaaca tcagagtttt ttagaatggt tattcactca tgcactcaac
19201 aaacacttat tacatagtat gtgccagcat aggggataca gtaataaaca aaacagacaa
19261 gttccctgtt catgtaaacc ttggacttat ggggcaaaaa gacaataaaY aagcagacaa
19321 aaaaaaatta caaacactct caatgaagga aaagagggta acatcgtaca ttgatggggg
19381 actataaaat taagtagtaa ggaaaagctg cacttgagaa ggtggtattt gggaagagac
19441 tcgaatgcct agaataagcc aaccagattt ggggcacagc actctcaaca aaggctgagt
19501 aacacagcca gtctgaggga ggacaggtct acggaatgag agccgaggca agtaaaggca
19561 acatcacgtg ggtcctgagg gtatgagcct tccagagggt gtgaggatac cattagaagg
19621 ttctgagcag ggaatgatag gatgtgattt acattttgta aagctgtctc tgtagaggat
19681 agtttgtaaa agtgcttgag gcaaaactga caagacttga agacttgcac aacagaactg
19741 aagaaatgag aagaatcaac tcctagactt ctgggatgat caatgtctag gatggacatt
19801 ggtgccattt gcaagatgga gaagaccggg ggaggtacgg atttaaagga gggtatgtaa
19861 ggagaaagaa gaatcctatg agacaagacc agaacatgta aatacatgaa gttttggatt
19921 tcttactctt caaagacatt ttacctcctg tccccgaagt tcttcacaga gttcaggata
19981 agaaaagctg ccagacagtt gagttttgaa taagtagtaa ctttcaaaca cttgaaagac
20041 attatattag aatagactac atccatcaaa ataNttaaaa atgtttttag aatagtccaa
20101 taatttggaa tatgaaagcc taagaaactt ccttatccaa ggccRcataa tataaccaaa
20161 attagaattt agtactccta gttggcaata tttttatgaa attactgttt tactaagaca
20221 ttttagaaat gaagacttta ctgaaggcaa taataagtgc aattaaaagt ggtacaataa
20281 Kaagacttct ttttcatttg caacaggtta gtagtgtatg agaattatga gatgtaaaat
20341 agtgtagagc ataggcttct ctagttggct tcatgtgagt tcgaatcttg gccctgcctc
20401 tcttaccagc aatgaaatat gcagcatttt acttaaggat actgggttgt atggagagaa
20461 tcagagttaa ggttggtgat ccagcagtgt catgtgtcag gagctaataa attgtattgt
20521 gaccagaaac actgaaactt gttataagga aagcaatcta attaaaacag ctgcattaaa
20581 atttctacaa tatcttccta acagcttatg ctatcaacag tttgtaaact atcagaatca
20641 cggatactgg ctgagaatgc ttttggagat ttcaaattgg cataattttc tcagaatgca
20701 ctgatctgac aaatgtacag acaggcagac aggaatacta ccactcttca tgaataactg
20761 tctgcctctc ctggatttct tccatgttgt gtaagtctat tacactttgg gtgaatgtag
20821 ctttagtaca ttgagttaat atcattcttg caaagaagct ctttataaat tccttaaact
20881 taatttgact ctggacacaa acaaatgaga acaaaataca gctcaaggat taagatcctt
20941 aaccaaatat gtaaaaaaca cgaaaagcct tacaaacagt gatcaacagt taaacatgcg
21001 ctatcacctt cccctaacag ctagggaagc atgtatatga atggaaeata tgaacttcca
21061 tttggtgtac taagaaaaca catcacgcag atggctttta tagcttccca aataaggttc
21121 taataattca caaaagtgtt gttccctaaa acccataata aacaataata aaacctaatg
21181 tttattgagg gtctactatg tgtcaaggag agtcctatgt actctcctgg aattacttca
21241 tttatttcta ataataacct ttaatgagca ctgtcactco cgtttcacag atgagcctta
21301 aggtcttagc tatcaacttt gcagacaaca cccaagtaaa atcctgcccc ctcttttttt
21361 tttttttttt aaccagaaat ccagtcctga caactgaaag cttatagggc atttccgctt
21421 agatacatag ccttacctaa attatatgtc taaaaactta actgtcatat tgtccagccc
21481 tcccccaata cccaactggc ttctcttcat gattttccaa ctataattct gtccattatt
21541 caggtttaga atttcatgct ttcttctggc tgtggtcctg ccaagtctta tgtatgctgt
21601 cacccttctc tgtgcaatct ttctcctact cattcctctc ttttcatcac tatcctattt
21661 caggccctta tcactcttac acctaattcc ctaacggttt cttcttgctt tcagtacaat
21721 ttccccttga tataatctat cctgcacact accaccagat ataaccttct aaagtcacct
21781 tattttagtt tcttcataat attttcctta tagtcaaatg taagggttct tcatgaatta
21841 taggacaatg tgcaaatggc ttgacctggc atttaagaca gtctgcagta tggcctcaat
21901 ctacatttct cacacctgcc actggtccta taatcacatc ctctgttcca gagaggctca
21961 ctcctccaga atgcccacta ccctcctctc cagcaaacat acaataaagc gaatccacat
22021 gaatacccca tcctaccctg ccttatccag cccctggaat accacctctt ccctattaga
22081 tgcctatata aatcctcagt attcaagatc cagcttaagg agcacttttt ctgggaccaa
22141 tcattctggt cctctgtatt gaatactcga actcctctac aggacaggaa cataatgtat
22201 tattacccta tcattcatta acacacaaca ggcttacatt atatggctgg ttgtattttc
22261 agaggtatgt ctatatgttt cgctatctat ctatctatct atctatctat ctatctatct
22321 atctatgtgt ttgaatacag atacataaaa atctcatttc acctataaga acctctggag
22381 cagatacatg tcatacaata attgggctaa aacaaaaatc cctcagtgaa aacctaaaga
22441 ctgggttgtg gttgtttgca gccaaaacgt acaaaatgaa aacctcctaa cgatttattt
22501 tccacatgaa attagtacca gatctagaga aaaatggaca aggatcaaat acaaataata
22561 aagtcatcac caaactaaaa atttcattca aaagtgatga tgttggccgg gtgtggtgac
22621 tcacacctgt aatcccagca ctttggaaag ctgaggtggg cagatcactt gaggccagga
22681 gtttgagacc agcctggcca acatggtgaa accccatctc tactaaaaac acaaaaaaca
22741 gctgggcgtg gtcatgcacc tgttataccc actactcagg aggctgaggc aggagaatca
22801 cttgaatcca ggaggtggag gtggcagtga gccgagattg tgctactgca ctccagcttg
22861 ggcaacagag caagactccg tctcagaaaa ttaaaaaaaa aaaagtgatg atgtcagtaa
22921 agacccaaat cagggtcata tgtttaacac cagaaagttt gtatttgcta caagacagaa
22981 accttgacaa aataactttt ctgtgctttg ttacttcatc tgaatgaaga aggtaaacca
23041 gttgatctca caaatctctt tctaagggct tctggaattt tttttttttt ttaaacaata
23101 aaaggaaaga gacttcYctt aagcagaaca aagtccgact cctctgtaaa gtggctgaca
23161 gcaagtggaa aatggaagaa gaacaattac ctgcaaaatg actcattccc ttgtaacaac
23221 tacactacta gggaattcct tctcttaccg gctgtggcta gcgtcagact catgacaaag
23281 acctcatcct ggggctagaa ctaggtttag ggttttctca ttcattcctc tgtttctcag
23341 ctgcattttc atttttaatc tgtgcaatga ttacagaggc ctatgtttgg ttttcatttc
23401 tagtttcccc aggaagagac aactactatc ttgatattga ttaacacttg aaacttctgt
23461 taatcacaat ttcagtattt cagggaatca gagaaatcat ttggaagagc cttgaaggtc
23521 atccagggtg agagaaaagc aaagcacaag caacagacca atgtttagac acagtgaagt
23581 gaagRcctac aggagaccct ctcaacttca ataagagtct tttttatggc tgtcttcata
23641 ttctttcaaa gaaatcataa taaaggagtt agtgagagtg taaataaaaa ataggctatc
23701 atagagtgct gggtaactga gctaaaacta aagtaaacaa actcagtggg ccatcacata
23761 tgcaagctgg taaggtctca taaatcacca tgactgggta ttgggatttc aaaggcacac
23821 tctgtaagct ttctcccagc cctgttgatt caagctccaa ttgcactggg ttacttatat
23881 ggcagcatat aaagttgacc ttttaYgttt aaaaatagtt ccattcctca tccctcctaa
23941 ggataggcca cagtacctat catatgtccc caccacacta cataatttat gtaagttttc
24001 acttaatttt caacacaatc ctttgaggta gattatgtta gtcctSattt acagggaaaa
24061 cagaggctca gggaggccaa atttatttat ttatccaagg ttgcaactct actgtagggc
24121 agatttatat ccagaagagg aattgcaaag ctcctcagaa tttaggagat ttaaaatgga
24181 atcaagataa aggttcacat ttttcatctt ctattccatt ttactcttcc agcatctaat
24241 aataacctat gatatattag aacaaatcag cccatactta ttttgacagt ctcttttcac
24301 cccatttcag tttgtacctt gctgtctcag agcccatcaa ccacctttcc tcactcccag
24361 gcagccggtc cttcagggga tggcttggca gttggcccac catcctctgc taccagggca
24421 tgactcatgg aggcaacagg tgaagctgaY gccaactatt agtgctaaaa aagactcaag
24481 cctcatgaaa tataccatac ataaatcgct ctccagtcag ctctacccct ccctccattt
24541 ctacagctga ctgtctgttc ttccttaaac atttacatcc gccactgacc tgtctgggat
24601 ttctttttta cttcaaggYt ctcctaaagg ttcagtMcaa ccaggtttca ccaaatgaac
24661 cctgcggtgg aaaggggcag caacgggcat tggtcagttt gcctccagcc aaggaaagtc
24721 tgactcccct ccttctccca ctcttctctt tctctgcctc acactgccta atacgtcttt
24781 cctctttctc tctttctttc cttatatctt cattttccac cctattcaga agtgaaactt
24841 cccttgccac tttgccccca ctaagaaaac agacctccaa cacaacctct taaYaggcta
24901 attttaaaat catgaacctg cttacacctt ccgtgagttt atcctatatc ttctctatct
24961 gctgtctgct tcccactaag gttttatccc tccatcccac ctccaaatcc tttacagctc
25021 agattctcaS atctgcttta gtcacttggt ctcctcatcc cactgaccct attatccttc
25081 tgtgcacatg ttagagggca catgctcctc ctaggaacta gggccaggtc agttatgctc
25141 ccatcctgac atgacctaga gaacaaaaaa ggcaaaacga ggtactgtag gattcctctc
25201 taggaattat actttgctga aattgctctt gctattttgg aatctagtgc tcactaaata
25261 catttttaaa atattatttg gatatatttt gaaaatacaa ccagaggtcg ggcacagtgg
25321 cttacgcctg taatcccaac actttggcgg gccaaggtgg gcagattacg aggtcaggag
25381 ttcgagacca gcctggccaa catagtgaaa ccctgtctct actaaaaata caaaaaatta
25441 gccaggcgtg gtggcaggcg cctgtaatcc cagctgcttg ggaagctgag gcaggagaat
25501 tgcttgaacc tgggaggaga aggttgcagt gagcctagat cacgccattg cactccagtc
25561 caggcaacag tgcaagactc taactcaagg aaaaaaaaaa aagagagaga gagagaaaat
25621 acaaccagag aataaacact tgttcttcta tctctccatc ttccccacca atacaacatc
25681 cagaaaatag tcatctttac acttatttag aagcacatag atcttataaa aStttaactg
25741 agcattaggt tttcatcttt aaaaatattc tgaggctggg tgcactggct catgcacttt
25801 gggaggccaa ggcaggagga tcacttgagc ccaggagttt gagaccagcc tgggtaacat
25861 agtgagaccc tgtctctaca aaaaaaaaaa aaaaaagtta aattaaatta aaaaacagaa
25921 aagccggtct ggtggcacat gcctgtggct ccatccactc aggaggctga ggtgggagga
25981 tggcttgagc cagggagatc aaggctgcag taagccatga tggcgccact gcactccagc
26041 ctggttgaca aggtgagaac ttgtctccaa aaacaaaaca aaacaaaaaa cgttttgaca
26101 tgtagaatac tgtttctggg actaaattag agatttaagg tttgaggtat gagaaaatac
26161 ctggaatcat gccttacatt agaaggtaag gtaaatactg gtgtttccca atgagttgta
26221 gtcagattgt tcatatgtat caaacagaca atatactgaa atatttaaaa ataaggctaa
26281 atagcaacaa ataatttttc ccatgaattc tacttttttt tttttttttt ttgagatgga
26341 gtctcactca gtcgcccaga ctggagtgca gtggcgccat ctccattcac tgcaacctct
26401 acctcctggg ttcaagtgat tctcctgctc agcctcccga gtagctgaga taacagacat
26461 gcaccaccac acctggctaa tttttgtatt tttagtacag gccaggtttt gccatgttgg
26521 ccaggctggt ctcaaacttc tggcctcatg tgatcgacct cccaaagtgc tgggattaca
26581 ggtgtgagcc accatgccca gctgaattct actttttttt tttttttttt gagacagagt
26641 cttgctctgt cacccaggct ggagtgcagt ggcacgatct tggctcactg caacctctgc
26701 ctcctgggtt caagtgattc tcctgctcag cctcccgagt agctgagatt acagacatgc
26761 accatcacac ctggctaatt tttgtatttt taggagagac gggatttcac catgttggcc
26821 agctagtctc taactcctga ccgcgtgatc tgctctcctc tgcctcccaa agtgctggga
26881 ttacaggctt gaggcaccac gcccggctgc tgaaattcta cttttgaaag aacaaattcc
26941 ccttctgtag ggactgatga ttcagtctgt aatgatgcat tctcttcatc ttcttttttt
27001 gtcttttctt ctatatatcc tttaggcatt ctactgtatt aacactagat agaaaaatta
27061 agatttaggc ccaaacatta tcttctggca taaagtaaac taatcKtttc ctgatgtaca
27121 ataaataaac agacatattt ctagtagaag attccttatg atttatcaaa acccaattat
27181 cacttaccct tatggagatc ttagatctta gaaaccatac tgacaatata agattaagaa
27241 aacaacagaa aatcaaaata tataccaagg ataatgttaa taggtttcat ccttatcaca
27301 gcatactgtt gaaacttact tgaaatattt ttttaaaaaa gatacaagag gacaagtgtt
27361 tagtttttaa caagggaggg gactagtcaa caaaYattga ttacctaatt tatgaacagc
27421 tctggccaaa gtggtcaaaa aaaaaaaatt aggactttag ttttcctaaa cctatcttaa
27481 tttttaacag gattatagct ttgttcaatc tttacagtct ttagcctctt ttgtgaaatg
27541 ggtttccttg taaataataa aatgacacct atatatattt aaagcactca tgaaataatt
27601 ttatctcttt attaaaaact gtgataaccc ttccaatttt gaaatttatt gtcttcctaa
27661 attcttgcta caaagcctat cattaatatt gcagttcatt ttttccctaa agattgaaaa
27721 aaacactaac aaatataaag tctttatggt caaagaatgt actcctttta taaaacttgg
27781 agttgatgta aaggcacaga caacacaagt taccttaata ataaatgcac ttttctttgt
27841 aattttgggt aattcagtgt gatttgtaat tcttttaaaa gacacacaat cacttgtatt
27901 tttgcatggt tctttttttc ctaatttccc actatagtga aatatctgtt tttccttctc
27961 tattgggttt ggattggaat gatcaggtag aattaaatgt aaaataacaa aaggaaaata
28021 ctttatagat cttctcaaaa tactttgaag ggtataatct ctttaagatg gctttattca
28081 tatttaagcc aaaaacaaaa agcaaccaaa acactactgt ttatacaaaa agcagcttca
28141 aaatcagcca gttattttct gaaatggaga tggcagacat tagcctgcac ttactccaat
28201 atggcagccg catctttgcc tctttgctat ggacacatcc ccagcaggcc tgtacttect
28261 actctccaga cctgtttatg acaggccaag gtgtttccta ttatggtgcc tatggctgca
28321 cagtgcactg cttgtcagta ataaaagcca gaggctgtcc ctatagggat tacagtgcag
26381 cagaccagag atgctgatgg gaatgcaagc atgccctagg caaacattgc agaatactct
28441 ctgacaggca aaagccaaca aacacatcct ttcttcccag actagcaggg aaagtattca
28501 gttatgtcat tttcacttgg aacaaactaa taagaagaat gttattgcta aaataggtag
28561 agatgagaaa gagcagttta tgaccatgaa tatggcctct atggtttttg cctgtgcact
28621 acaaactttt aatttaaacg agatgagatt gatctagcat atactatgat aaaatttctg
28681 agacatgagt atctaaaagc agaactgaga ttaagtgaga aatgctcagg attcctgtca
28741 ctgaaagaaa aagaaaaaaa cctttcttga aaggcacaaa gtagaatgct cagactctcc
28801 atctaccaat acaaggtaaa aaacagcatt tgcattaacc aacatcatgc attcttgtac
28861 taatttatgt gactaactac aaaatgttat attgacactc actgtgtagg gtgtcaggat
28921 ggccaagtgg tctaaggcac cagacactca ctgggttggg ctaaaaacac Ygtagaagac
28981 ctcatcagaa caataaaaca gagacacaaa atattgtact aagagttaag agaattacaa
29041 ataaaagttc tgtggaaagt caaaggtatc caattaggaa agcctgaggt gggagacata
29101 ggacacccca gatataggat gggaagaaga tgggattaaa aaaaaaggct ttataaagga
29161 tgtaacattc cagataggcc ttaaaagatg gcttcgatct tttaaataaa actttttgtt
29221 ttagaacaga tctagattta cagaaaactt gtgtagacat tacagataat tcctatatac
29281 tctacactgt ttcccatttt taatgtctga ctttagtatg gcacatttgt cacaattatt
29341 gaaccaatac tgatacatta ttattaaaca aagtccacac ttcattcaga tttccttagt
29401 tttcacgtaa tttccttttt tctgttctgg atcccatcca ggataccaca taatatttag
29461 tcctcatgtc tcctgaggct cttcttggct gtaggtatct caggttttcc ttgtttttga
29521 taaccttgac agttttgagg agtactggtc aaatactggt caagtttcga gggtagaata
29581 tccctcaact gagatctgtc tggtatttgc ctcatgatta cactggggtt atatgttttc
29641 agtagggaaa ccacagatta aaagtgccat tttcatcaga tcatatcgag ggtacatgtt
29701 atcacttatc actgtcaatg ttgaccttga taacctggct gaagtagtga taggWttctc
29761 cactacagtt tctttttttt ccctttctac actgtactgt ttagaaggaa gtcaatatgc
29821 acagctcaca cttacggtgt agggggttat gttctccctc cttgagagta gagtatctat
29881 agaagttatt gggaattccg cacaggagga gatttgtcta ttttccccca cttaggtatt
29941 tatttaataa attgtatcaa tatgggttta tggatattta ttttaccctt tgggatataa
30001 tccaatacta tattattttc ttgctcaaat tgttctagct ttggccattg ggagctcttt
30061 cagtttgctc ttgcaactct tcagtatatt cccacgggtg tagggttcca tttgtttgag
30121 caggtcctta ctttttggca ctacaagatg ctccaggctc atcttacata tttccttcct
30181 gagtccctag aaatcagcta ttttttttca aagagtcttg gttcctttta ccagagaatg
30241 gtattagaaa ccaaaatgag ggtgctaagt gtgctcattg ctactggggt atggtagctt
30301 ctagaccctc tcggctgata gagcaaggaa atatttgtgt gtatactaga tggtaagatg
30361 ttgagaggta gaatggagag ttaccttctt aatatacttt tcttaatatt aaatattaat
30421 aagatattgt gtgcctgggt tactttcagt cttatgcaga ggaagaacgt atgtacattc
30481 atttatgaaa aacactggct agactattct tctgcactgc attatgcatt tctgctagaa
30541 aaagctcaga gattttcaga tattctgaca aaaatctcag gcttgcttga tgagctatac
30601 tgtaaaatta cagaaggaaa caaagggcaa actccttgaa ggatgaaaat aaaggaataa
30661 aacatataaa acaccaccaa aaaaagtgat taatggggga gaagagtaaa taaaacatta
30721 taatagtcct ctttggttag tatcaatatt attaggtcac tttatcaaaa ggatagaaat
30781 caggatggca gacacccttc tctaaccctc accccccaaa ataataaaat aaaaactaat
30841 gttattcctt cacatatgga aatgaatgag ttctactgag tatctagtat gattcctgac
30901 tcagtcgaga caattcccat cttttaattc attaaatcac cacagcaact atttaacaga
30961 tgagaaaact gaagcacaga aggcaacRtg gcccaattta cgtttggtaa aaggcagaga
31021 atgaatccaa ttcaggcagg ctgtctatag tagggtgctc taaatccacg gaccattctg
31081 cctaaggaat gaatatttta tgaattggaa tatttctgct gaacgaattt gccattttct
31141 cctctagtgg agaaatccaa ctgagggtaa agaaggaaaa atgttgattg tttaaaacaa
31201 taaattagtc tctaaattgt tcttgcagct gatttagact agtgtgtcca aaagtatgcc
31261 cagtgttagt aaatggtagg caaaatgctt taaggtggtg caagatgaat gttttctcat
31321 tttgttattc attaatttta gaaaaaatac agaaaaaata taacttgaac atcaaaccta
31381 tgatttcatg tataccatta cttaagatga ggcacttggg tgagtattta gatttaacaa
31441 taagttgctc ttagcatttc tatactttga caataaatta tccaaaaaag aaattaagaa
31501 aataatccca tttgcaatag catcaaaaaa atacttagga gcagccaggc gtggtggctc
31561 actcctgtaa tcccagcact ttgggaggcc gaggcgggca gatcatgagg tcaggagatc
31621 gagaccatcc tggctaacac agtgaaaccc catctctact taaaaataca aaaaaaaaaa
31681 aaggcaggtg tggtggcagg cgcctgtagt cccagctaat tgggaggctg aggcaggaga
31741 acggcgtgaa cctgggaggt ggagcttgca gtaagccgag atcgcgccac tgcactctag
31801 cctgggcaac agagcgagac tccatcMcaa aaaaaaaaaa aaNaaaaaac ttaggagcaa
31861 atctaaccaa caaggtgaaa gatctataca ctggaaacta caagacattg atttaaacaa
31921 ctgaagaaga cagaaataaa tgaaaagata tcccatgttc atgcactgaa agaattagta
31981 atgttaaaat gtccacacta tccaaagcca tctatagatt caatgcagtt cctctattga
32041 ttcaatgcaa ttcctttcaa aattccaatg tctttttttt tcacagaaat agagaaaaaa
32101 atcttaaaat ttatatgaac caaaaaaaga cctaatagcc aaagcaatct tgaacaaaaa
32161 gaacaaagct gcaggcatca tattccctca tttcaaaata tattataaag ctactgtaat
32221 caaaacagca tgttattgac ataaaaacaa acatcaacca atggaacagg atagaaagcc
32281 cagaagtaaa tccaagtatt tacagtcaat tgatttttga caaagggtcc aagaacacac
32341 aatggagaga ggacagtctc ttcaaatagt gttgggaaaa ctggatgtcc acctgcagaa
32401 gaaagaaact gtactcttat ctcacactcc atataaaaat caactcaaaa gagatataaa
32461 ggcttaaaca taagacctga aactgtaaag ctactagaag aaaatataag ggaaaatctc
32521 cacaacaatg gtctgggcaa tgatttcttg gataggactc caaaagcaca ggcaactaaa
32581 acaaaaatag acaaatagga ctacattaaa ctaaaaagta cctgcacaag caaccaaata
32641 atagagtgaa aagacaaccc atggatcagg agaaaatatc tgcaaaccat acatctgata
32701 agaggctaat attcaaaata tataaaaaat tcaaacaacc aaatagtaag aaaacaaatc
32761 gattaaaatt agRcaaagga tctgaaYaga cacactttcc tcaaaaaaga aatatgaatg
32821 gccaacagag atacaaaaaa aaaaaaaaaa aaaaaaaaca atgttcccca gccctgcaca
32881 gtggctcatg cctgtaatcc cagtgctttg ggaggctgag gcaggagatc acttgaggcc
32941 aggaattcta gacaagcctg ggcaaaatag caagaccctc tctatacgaa aaaataaaaa
33001 gccaggtatg gtggcacaca cctgtagtcc tggctacttg ggaggctgag gcaggatcat
33061 tgagcccgag aatttgaggt tacagtgaga catgatcaYg ccactgcact ccagcctgaa
33121 caaaagagca agaccctgtc tcaaaaacaa acgaaaactc aacaatatct ctaattctca
33181 gggaaatgca attaaaacca caatgagcta tcacctcatg cctgttagaa tgactatttt
33241 cctttttttt ctcttcaact tttaagttca ggggtacatg tgcaggatgt gcaggatgtg
33301 caggtttgtt acataggtaa acatgtgcca tgtttgcttc acgtatcatc ccatcaccta
33361 ggcactaagc ccagcatcca ctagccattc ttcctgatgc tctccctgca cccatcccac
33421 ccgccaacag accccagtat gtgttgctct ccaccatgta caatggctat gttcaaacag
33481 atgaatgata acaagtgttg gcaaggatgt ggagaaaagg gagcccttct acactgttgg
33541 tgggaatgta aattagaacc accattattg aaaatagtat gaaggttcct caaaaatcta
33601 aaaatagaac taccataaaa tccaacaatc ctacttctag gtatacaccc aaaggaactg
33661 aaattggtac atcaaaaaga tatttgcact cccatattca tttcagcttt atttacaaca
33721 gccaagatat ggaagcaatc taagtgttca tcaagtgtgc ataaagaaaa tgtgatatat
33781 atatatatat atatatatat atattatata tatatatata tatatataga ttatacaggc
33841 cgggcgcagt ggctcagatc tgtaatccca gcactttggg aggccgaggc gggccgatca
33901 caaggtcagg agatcgagac catcctggct aacatagtaa aaccccatct ctacttaaaa
33961 atacaaaaaa ttagtggggc gtggtagcgg gcgcctgtag tcccagctac tcaggaggct
34021 gaggcaggag aatggcatga acctgggagg cggagcttgc agtgagccga gatcacacca
34081 ctgcactcca gcctgggcta caaagcgaga ctccacctca aaaaaaaaaa aaaaaaaaaa
34141 aaaaaaagat tacacagcct tataaaagaa agaaattctg tcattcacaa catcatggaa
34201 tggaactaca ggacattatg ctaagtgaga taagtcaggc acagaaagac aaatattgtc
34261 tttaataaat gatctcactt tatggggaat ctagaaaagt cgatctcaga aacagagagt
34321 agaaaggtgg gttaccagag gctgtagagg aggagggaga gagaaatgga agatgtcgat
34381 caaacagtac aaaatttcag ttagattgga ggaataagtt ttagtgatct attgcactgc
34441 atggagaccc cagtaattaa taacgtatta tatgttttac aatttctaaa atagattttt
34501 gacattctca tcacaaaaag ataaattggt gaggtaatat agatatgtta attagcttta
34561 ctaaatcttt ctatgatgta cacattttaa aaatcacatt atacccaaga aatagataca
34621 attaatattt gtcaattaag aataattgac aaataaattt taaagattaa taaaaaatac
34681 attaagttaa aacattcaac atataatagc agatatgaat gtacacagaa atgacaaaat
34741 cccagcactg tgggaggccg aggagggagg atcacgaggt caagagatcg agaccatcct
34801 ggccaacatg gtgaaacccc atctctactc aaaatacaaa aattagctgg gcatggtggc
34861 atgcgcctgt agtccagcta ctcgggaggc tgaggcagga gaattgcttg aacttgggag
34921 gcggaggttg cagtgagctg agatcgtatc actgtactcc agcctggcga cagagcgaga
34981 ctctgtctca aaaatgacaa agtttgagca acattaattt ggactgtaaa ttcttaaaga
35041 atagaaaagt catgtttttc ttctaagaaa tattatgttt ttacattgca agaaatatgt
35101 gacacagagt ttttcttaat ctctcttttt tagtatattc tagacaggca agcacatgca
35161 atttattgag cacccagtgt gtgctaatgc accaaacgga gctttacata cattatagct
35221 aatggtctca ataacttttt cattttattt tcccctttca ttatcgccac ttataaatga
35281 aaaaactgag ctccagagcg gtaaggccta ttgcccaaaa tattagatag gaaacaacag
35341 agctggaatt tgaaaccaag tttgcctaat gaaaaacatt tatgttcttt tcactgtaca
35401 aagctgctgt aagtatcact ggtgcctact ccaggccagg gagtaaatat cattcatcct
35461 tatcacatac aaagtataac aaaacaaaaa caagaagctc tgaatactaa ataataataa
35521 taaatacttt ataaaagata aactattctc taagagtaaa agataataaa cctattgtca
35581 ttgtagttgg tcactgaact taactaattt cctaatggac gcagcagaat tgtttgttgt
35641 ttaagcaaat atcaaggcat accccccaat ccctgttaca gggaataaaa aaagtcttct
35701 tcaaatgcct ttcaaaacta atgtgaatat taaaataaag tgctactgat ctctctacat
35761 tctagaaaga tctcagacta acagtgtcaa ttaaactatt acaacaaata gccatgtaaa
35821 aactgctccc ctactagagc tacgctatca caaaattaac cccagcaatc tacagttcta
35881 gaaaaagagt tggtccctcc tgctgaaaaa gagaaaacca aaccctacat attttgtgaa
35941 tccactgaaa ataatgacat aaacagcact gcacatccat catgtattta tatcttaagt
36001 gatagctaca gtctatgtaa ctatcgcaga aatcttctta aactgaacaa aagcattatc
36061 tatcatcctt tctctctaga aaaataccag tttgtttaat ctacccataa cattagaaaa
36121 aatacttctt ttatcatggg caatccttat gtttcggaat ttttttcgaa aacgtagaca
36181 gttcataatg ctctcagaca gaagttacca ctactacccc aaatattaag tcaaaatgta
36241 actgaaaaaa agaaatttat ctagtaatga ttaatttatt gaataattaa aaattaggag
36301 tttttgataa ttaaatgttg ggaataatta aactttcact aaatactggc acaggaactg
36361 aacggatgac aacagagaca agttaagatt tgttttaatc ctaagtttta tgctaaggta
36421 acatcaaggg ctctactttt cagtgaaaga aaaatttcct ttaaaattgt ttccctcttc
36481 cattattact taaaagcaaa gaaagtattt tttctaacag tgattatagg attctcaatc
36541 tttcatatta actttaaggt actacatgta agctacataa atctttccca aatctacctt
36601 tattgagtag atgaaaaagc agatatcgaa ctcaaaacat ctaaatgttg attttctata
36661 attatagaag atattttctt ttgaaattta aagtttgtat ttaggcatat taacactttt
36721 ttaatctaag atttaatatg ttaatgtaga aaacaactga ggctgttttc tctctataaa
36781 actaacatgc cttttccaat taacttagtt tccttcttag caaaagttta acatttatat
36841 ctgttgagtt acaYtgtagt tgcataatgt ggaaatatct taagatttag ccacttctat
36901 gaagatccta tatttgttat aacacccctc ttgcccagaa gaaaatacat caacaaaaag
36961 tcctactata tgttctgcat tgactattac aaagcagtag ggggagcagc cagagaaagc
37021 tctgagacaa agcacccagc aaagcaattt aatttatagg caacagaaac atgctagaga
37081 ggtttgccaa aagccaaata cacaaaataa attccataaa gaagtcatga ccacatttgt
37141 aaagctggcc taagtatatt tggaatgaag tatagaaaaa tctgaagtct cttagaaagg
37201 gtctggattc taacttcaag acagactttc agcttgaaac acagtcattt ccagccatgc
37261 caaacctcaa actgccccca aaattagagc agaaacaaaa tacattttta tagtgatatg
37321 tcttgggaat aactcaaaat gatttcgttt tctcctttga acctcactga acttacaggg
37381 aaggcctggt gaaatgtgca ttattaggtc acaagaggat aacgttacag ggttccatag
37441 cataatcctt gaaagccctg aggcccaggc cgctgtcctg ctctcctctc tactgactcc
37501 catcctcaat atactttcca tgagagaaag gtaaccaaaa acatggtgaa actattctgc
37561 ttaaaaggtg ttttactgaa aaggcacatt gaccactaac ccttattaca tttcacatgt
37621 tctaaaaatt agtaaggtct gcattttcag ttttagagta aaacaggaaa agtacttagt
37681 tagctcttct gtctggcatt ctgtaactgg aatttaagaa aagcctaaac ttctaattag
37741 aacttcagaa tgttcatgtc tgtattatac cacaaaaact gtaaatataa tttaagcatt
37801 tttatgatac agaaatgtgc gctatagaca gttatctaag aattataaaa atccatcaaa
37861 cttgaagtgt ctggtataaa ttacaaccga atggtaaaaa gacattttag ccacgtaggc
37921 aaccaatgaa gtttgaccta aaacccaaat aatagcaata acaacagata cagtgcctac
37981 tattgtcaga tactatatta gctagctctt ttgtatatat tctttKgaac tcttacactg
38041 acttttcaag atagttatta tggtgcccat tgtatgctaa ggaaactgaa gctaaaaaaa
38101 attgaagtca tttctctaat gtaactccat tagcaagaaa caaaaccata attcaacttc
38161 caggtttgcc tgcctccaaa gctttgagac ctcactaaaa ttaccctcaa ttgtagcaat
38221 gaggttccaa aggaaatctg tacaaatctc aggcagatta atgtgcttat aagggtaaat
38281 gccatagatt taatcactca aattaaatgc catctggaat ctcttaccct acttggtaca
38341 agcatcctac atgtgttaca gccaacccta gccttggcca agaattcaat aattttYctg
38401 cttcaatgcc atcaacaagc aagaaacata tataatacca atacaggaaa tacagctagt
38461 tgctcctaga gcatcattta aaagctgcta attggctaag atgcctttta ttgagaagtc
38521 taagaaacag aaataatgtg ttaacccagt ttgttaaatg ggtttgagga atcagtttcc
38581 tccttcctaa tacctaaatc catctccata ataagtattc tctatcacca tttaaaaatc
38641 ctgatctctt actagtgttt ttattgtgct tttcaatctR ttagagtaaa ttttttaaaa
38701 cctcaagaga gaacacagaa aactgtcata atttttcaaa ggctaaagga gaataaactg
38761 aaaatatcag tttgaacacg tgtatgtaaa aagaatggat ggtaataaaa ctaacaactg
38821 aaaagcaatg tttctgactc attcattcat actcaagaaa tatcaaataa ctgagtggag
38881 atgatgcaag ctgtacacat ttccagttca gactgaaggt gtggcaacac tcacaggggc
38941 cttccgatca gctgctccaa ctcagtttac agcttttgtg gatgtggctt attcatgccc
39001 ttgttatgaa gaagaagggt aactttacaa cctttagcaa gagctttttt ttctttctgt
39061 gctaaccctt ttttgtctat aacagtcatg taagcttagc actgatttag aaataaataa
39121 gttaaagaag acacgaatgg cttggacatt aaaagtttaa gcaacagctt aagctgagct
39181 aagtttaatg ttaaaaatta gtgtgacagc aataattttt caattcagct ctgtcgtggg
39241 ctaggaaaaa atatcctaat ttttcagcca caagacataa acattgctag aatgactaca
39301 ttttgaaaaa attatcttct cactttacct agatttcatt ttaaccaaac agaccaacct
39361 ctcctattaa acctgtaaat taactcaaag tctaatctgg ggccttcata ataaagattt
39421 tttttttaaa tccctgtctt atctggtgcc tttaaaagaa aggaatcatt ttaggcaatt
39481 attatgacct aaaaaatttt taaatcttaa cgtatagatt aattccttga cagctgaagt
39541 catttacaat acattattaa atttcacatt cagttttaaa tccaaacata gatctcaaca
39601 ttaagaatat ggattttagg gaaagYaact agctgctcaa attcatttag ctgaaaaact
39661 actgtcgaat caccttgaaa aYatacacta atgcttgcac attgctaagK ggcttttgat
39721 tctgctcttc attttcttac acagMatcaa ccacacaata gaattaagaa ctcaggccca
39781 tggcaaagtg ctcccattta gcagttagga cactggacat attcagcata tgtctactgc
39841 tattgagaat caaattatga aaacaaacta tattttataa aactaagaat ttgttactca
39901 Ratgagaata aaggtgccta atgcYacagc agggggtaaa agtcccagta agagagcaat
39961 aattggtcat tctgatgagg agctgtgcca aatttagaaa tgaggcacaa atgacaaaaa
40021 aattgatcat cagtgtggac atgggtaggt cagtgtgcat atgggtaggt tagtatctgc
40081 tggccgtgtc tacagagccc cttttattta tttgaagcca acttttcact tgacaatgct
40141 acctacaaaa gcactgttaa tagataagac aatcaagtgg ctgttatgcc gtggcctttt
40201 tgtaaaaact gcatgagaac aggtgggata aaaaccatag aaaggtagaa aaaaaaaagc
40261 atcactcatt taaagatatg tgcacataga tgaactgtaa gataatataa ccttcttgac
40321 cttgtaaata ggtggccaaa aatacttaaa tatctKgacg aggcaactat tactaattgt
40381 ctcacaacat taYcagtcct tactggctga atccttcagc catctgcata cgacatgcac
40441 aggcaaatag ccactaaaaa catacacaat tccaattatc tccttgcata actaaagaca
40501 aaaagaacag gaaatccaaa aaatacagca aaactgaaaa cactatacct tgccttccgt
40561 gctattttaa ggctgtccca ggctgaaggt tacttctcag ccctcaaaac tccattaact
40621 gccaagacca gcaccttagc acatatggca cagatgccta gttgaagcct ccttgtacta
40681 aatgacctca aaaaactggg ctttctttcc aagattaatg gaaacaaaag taatgatcat
40741 aaggaacctt ctaactctgt taatgtttcc tgcggatatg tatggccaac tgaatatata
40801 aaggctcagt tcttgcgggg taccctgaca actagatgaa ttagggtgaa ggttccaagt
40861 tcgatgaact cttctgagtt tgagtgccct aggtggtgac attcaagtga tttcacagac
40921 atccaccaat tcttattttc aaccgggaat cccaagaaca cctttaaaca tgcaatgccc
40981 atggacccta ctgggtttct gctgcaaggg ctcagtgtta cttaggaacc ctgtgtcttg
41041 ggagatactt aaatttagca cgtcaaattg cttttggaaa atgtttatct ggcgtgacta
41101 ggggaggcaa agcaaggaaa caggaacctc cctctaagga gcagaatagc caacatgcag
41161 taagcctaaa aaacctctca atttatttcc cggggctatg ggtaccagtg gctcgccggc
41221 atgggaatcR gcagcctggg ggcaccagtg acagcagaac cggggcttac cttgttggag
41281 gccatctcac tgacggagtg cctggtctgt agggtctcta gctggtccag acaccagtcc
41341 agctcctcca gggtctcgct ggccagtttc tggtaggcct cctctgcgaa gagacaggga
41401 aagggggact cagttctcaa gcgcttcacg ggtccgctag cgagttcaaa gggggccatc
41461 ctgccatacc ccgccatgct cggatgcccg gtgccggcac atgagggctg ctccttcata
41521 ttgcccagcc cggcagtgca acgggggcgg aggctgtgct cgcggggcgg ccggcctcgg
41581 ggaggcacgg tctctccagc ttggcgtctc gggtcggcct ccagggaggg cgtgcaaaga
41641 gcggcgagcc aactttcaca cagcgacgcg agcggaaaaa ccactatgcg acttttcaac
41701 tgtgcggcgg gcccgagtcc cagtccagga gcYcggctct agcggatggc gagggggtgg
41761 cgagcgcgct tgggtgcccg ccccgcagag gagcgtctgg tccacagccg gatctcctcg
41821 gtccccaacc cggagccgcg gccccacgcc cgccccgcct gccgagcctt ctgcacKgca
41881 cttgaagtga atgaatcagc cgcggccggg tgcgcggcca ccacgtttcc tgttttcttt
41941 ctcaactcct cgggcggcag gcgcagcgcg gctccaacgc cgcagctttc cgggaacact
42001 ccccctcacg cccctctcgg ccctcggcgc tgcctggctc ctcctcaacc cccttacgaa
42061 aagattccga attgcaaaag cctgtccctg tccgtgccac ccgccgcgtc cttcccctcg
42121 agctctgccc cgggggcccc gccacctcgc agtgccatcc ccccggcgca gccgggcgcc
42181 actcgctttg aagtgcagac gaggaggcgc gggcgcgagc gtgtgtgtct gtggggctgc
42241 tgtgggactg tctgtgctag gagagcggcg gaaaacccgg cgtggagcgc ctgcagtccg
42301 ccgtccgggg tcgcgcccgc cgcggggctc aggagggacc ggcacgcagg cgcgcacggc
42361 cgcgcacctg tctcgctaga accccgcgcg ccccgccgag ccactgccct ccaggttgcc
42421 cagaccgctg gagcctctgc gactccacga ccggctgctt cctgcgaagt ttcacaaaca
42481 aggttattta ggaccctcaa ggtttctgag ggtttaagtt ttagggtttc tgggggttcg
42541 tttctaaagg cttaggattc acctacttca aggaggcagg gttgtgggaa ttaagaccgc
42601 attctttttc tacccagccc agtccctact aagtaccaga cttctaaatg ttggggcgga
42661 cagaaacaag caaacaaaat atacaatgtt gtggggtcgg aacgctttat aattcacaaa
42721 tttgacaata ggctgcattt tcatatgccg aattgcctaa gagttctctt aaaggaacag
42781 caatgtgaaa gaaccacatg gtaaggagaa tttacgcatg tcctgcatac taotcctttc
42841 ccctgtgaat tgcataagac ctagatgatc ttgggccaag gaagaaaatc tggaagggtg
42901 tttctaccca ccatgtgcag agtacactac aattttgaga aaacggaaag accatattca
42961 aaggcataca gaaaagcctg ttctgtttac aaaggctgac ccagggcacc agggacactg
43021 tccctaagca gatcagttgt gggagaactt ttctacatta gcatgcaata agcaaaccat
43081 tcatagtgag gagaagtcaa actcccaagg gtccatttga taaaactttg tcattgtcga
43141 gtggaaagaa gatactgaga aacttacact aagtggtggg atcccttgtc tttgtctttg
43201 aatctacagg tattggagaa gaaattctgc ccttgaaatt tagactccat aaattaacat
43261 ggcacaacaa ataaagttca tggactgaat ttaggctcct gggatttttg agatagcttt
43321 ttatgtgaac tttgtaaaag ttagagtaac ttttaaagta gttgttacat gtgccacgtg
43381 gcttattgtt atcaagaata cttgaactat ttactgttca ctttgagtac tctaggttaa
43441 aatttgcctc ttttttgttc tacttcctcc accccgcagt ctatgtaaat atatacttta
43501 gatggacaaa caactgccta aaccttgcca ggtgaattaa agtttcagat tgaaKctttt
43561 ttttccccct taacaagctt aaagatatta ataattttaa atggtaaata taaaatacct
43621 gctctgtata gcttccccaa ttctaagaat gtaaaaaata ttacatgctt gttataaaaa
43681 ttgaagcatt acacacaaat ataggaagtc cattgtaacc ccacacccta aagatcacaa
43741 acaatacttt cgcctatggc tctaacacaa tcattttcat tttactgaat aacttccata
43801 tgcaagtata gcatcaccta gttgccatgg tagtgcatct actatatggt ttggttttga
43861 tattttcctc tctctctcat tcccataata taggaatgta ggaaatttta agaggacatt
43921 gacttgtttt atgatcattt gagctgtcca tagttagcct atccagtgga ttaaagatgc
43981 ataaccattg tgtctgggat ttgttcagtg ccatcttttc aatcattggt tacaatgaag
44041 ctgggagtcc cctctgaggt tcggcKttct gtggctcctc tcccctgcac tccaactttc
44101 tcaaagcctg agcctcaagt gatttcccag tagYtaagta aactgccccc ttcctccatc
44161 ttcaccaagt tcccaaagct Kttcagactc aggcttcaaa tctaacagga tgtggctcca
44221 cacattatat ttcagttgtt attttttaat gtggaaaata attcaggaaa gttttggcta
44281 atgcttcaag acatcatgca ctttatacac aggatttgtt cttaagaagg tagtatagtg
44341 acaggaggaa caatttactt cagaatatcc attttatgtc caagccatgc accagccact
44401 tttgacagac aggatttgag tatgatgata aaatggggta agcatatatt ttaagttcta
44461 acatctgtat aaataagaaa aatgaaaaat cactggaaca catctacatg ctagtgagaa
44521 aataattact aacagagtaa agtttgctct gtgaaactgt tgctcaaata aagaattatt
44581 cttgatatta gcatagagct gagaatattg cttttttcca aattcctagg tgagtaggca
44641 atatagctag ccacgatttt tcaggatcat ttgtaaagct tcacttttag gaattttaac
44701 cccaagtgtt ctaagacatc aagtaactga ccttttcatc ccgccgaaca aacctcaaag
44761 tggagttgcc aactatccca caggaaattg agtcaccaca ccaagcaaca cacctaaatg
44821 tcgacagcta gcaggacaaa aacagtgagc cagtttaatt ttggctttta ccaagaacac
448B1 atactgcatt cattaataaa atacttcccc tcaacccctg ccagagtagt atacactgtg
44941 gctctcctta aaaatacatg aaaactcttt aactgcacca aatcttgcta agtcaaggaa
45001 ctaaacagag aaaaacaccc aMgcaaaaac tattggctaa actccctaaa ggctgggaga
45061 gggctatgga acagcaactg agaaataaat tgagccttga aggcccctgc tacaacagca
45121 tcagttttct tcaaggcacc tttgaactat gtaacacccg cattctgctg aggctcttct
45181 gtgaacaaac agtcctcaat tcttgdgggc cagcacttgg cttctgtcac agtgatatgc
45241 ttagaggttc ccctgacaca tggcctgaaa ctgtgccagg ggctccagga gaagatgctg
45301 aagaagcagc ttccttttga aaaaggaaaa aagtaatcac ctcagagcca cagcagttag
45361 ctagaggttg aggtcatttt ttgtgaaatg ttcagtccat tactcttacc cacaactcac
45421 agtgatgttg accattgagg taaaaggatc cactgtacgg gatgtgtaac tatgtggaca
45481 ttagtttctt tgccaacact tgaaggtctg ctaaaggact gtatcggctt gtcaeaattc
45541 ctagatagac ctattggtat acatgaagag aacaagtcca aaaaatacca cttattttgg
45601 acactatttg tatgccctta atttaaaaaa aaaatacaaa ttataacagc tgaggtcggg
45661 cacagtggct cacgcctgta atcccagcac tttgggaggc caaggcgggc agatcatgaa
45721 gtcaagagat caagaccatc ctggctaaca cagtgaaacc ttggctctac taaaaataca
45781 aaaeactagc cgtgcatggt ggcgggcgcc tgtagtccca gctactcggg aggctgaggc
45841 aggagaatgg cgtgaacccg gcaggcagag gttgcagtaa gctgagattg ggccactgca
45901 ctccaacctg gacaacagca gaagactccg actcaaaaaa acaaaaaaaa ttataacaac
45961 tgatccagat tttttttatt attacacttt aagttctagg gtacatgtgc acaatgtgca
46021 ggttagtttc atatgtatac atgtgccatg ttggtgtgct gcacccatta actcctcatt
46081 tacattaggt atatctccta atgctatccc tcccccttcc ccccacccca cagcaggccc
46141 cggtgtgtga tgttcccctt cctatgtcca tgtgttcgca ttgttcaatt cccacctatg
46201 agtgagaaca tgcggtattt ggttttttgt ccttgtgata gtttgctgag aatgatggtt
46261 tccaatttca tccatgttcc tacaaaggac atgaactcat cctttttcat ggctgcatag
46321 tattccatgg tgtatatgtg ccacattttc ttaatccagt ctatcattga tggacatttg
46381 ggttggttcc aagtctttgc tattgtgaat agtgccacaa taaacatacg tgtgcatgtg
46441 tctttatagc agcatgattt ataatccttt gggtatatac ccagtaatgg gatggctggg
46501 tcaaatggta tttctagttc tagacccttg aggaatcgcc acactgtctt ccacaatggt
46561 tgaactagtt tacagtccca ccaacagtgt aaaagtgttc ctatttcttc acatcctctc
46621 cagaacctgt tgtttcctga ctttttaatg attgccattc taactggtgt gagatggtat
46681 ctcattgtgg ttttgatttg catttctctg atggccagtc atgatgagca ctgatccaga
46741 ttttttaaaa ataaaagaac tacaacaaaa agctcctaag agcaaatact taaatctccc
46801 taaccagttc cagcttctct tctcttaatt cttacaaaat ttcccctttg ttctaMggaa
46861 ggaattgata ctgtctacag tagatagaac tggaaaaaat gatttctgtt tcaccgtgta
46921 ttgctttgag ctagtcattc ctttagtttg ctacagtaaa agatttgtgt aggttaggtc
46981 taacttttgc tgacttaatt ttttttcctc tatacatttt atctgatggg aaatacatga
47041 aggctttgga ttctctttca atgtgtacat gtaataggtt taagcagagt ttgctagtgg
47101 caagctgctc tcatctccaa gtgtatttct ggcatcaaat tattgttttg aagtatttta
47161 aaattgtata aaatatataa aactttattt agtacttcat aaagcttcca aaggaaagat
47221 gtgtgcaatt ttgtgacggt tttctttttc ttttatcttc tcactaacta caattgtttt
47281 tccgaatgtt gcctatgcct ctagtagaat ttttatatga aataaacatg aggttggctt
47341 tcattgactt ttagtcccac cagtaactca acatttattt agcactgact atgtgccatg
47401 cactttatta gaggctgcag aaacaagatg atgaaaagag tgtgtccctt acccagagtc
47461 cagcagagga ggtaatttca gcaaacaact gtagagtgca ctctcacaga aggaagcatg
47521 tgggctatgg gacagtaaaa gaggactatt taaatcagac agggacagag gtggagtggg
47581 aaaaggctga gtcctcaaga atgaaataaa agagagtggg aaggattttt aagagaaaag
47641 atgagcaagc acacagacct atgatgaaca agatgtctgt gtcggccagg cgtggtggct
47701 cacgcctgta atcccagcac tttgggaggc cgaggtgggt ggatcacctg aggtcgggag
47761 ttcaagacca gcctgatcca catggagaaa ccctgtctct actaaaaata caaaattagt
47821 cgggcttggt ggcacatgcc tatattccca gctacttggg aggctgaggc aggagaatcg
47881 cttgaacctg ggagatagag gttgcagtga gcagaggttg cgccattgca ctccagcctg
47941 ggcaacaaga gcataaaaac tctgtctcaa aaaaaaaaaa aaaaaaaaaa aagatgtgtg
48001 tgtcaaaaaa gaaataacca tgagtagggc cttgcttagg ggaacagatt ctggagaaat
48061 ctgagaaatc ctcagccact ggccatctca aagactggcc atgtcaggca tctgcaggta
48121 ggactatatc ttcaagagta ggagtcacag agtgactcag agtactgtgt gacatgggat
48181 ctgattttat tttatatgga aaattctagt gctatgcaat taatggaatt Kaagaaagta
48241 aagttgaagt cagtgcaacc attatctttg accatattaa gaataaaatt aaataactta
48301 tagaccattt gcttctctga cgattattct tatctaagac aatctctgta ttaaaatcct
48361 acgcagacat atgttcccac aatatgtcag gtatgaacag aaggactgcc caatgtgact
48421 tgaaagggag tggacttctt gctcttaaaa actacaacct tgtagtcact aacttattaa
48481 tcatactgta ataagatgga gaaaaaaaca atggaattca ccagaatttc tttcacgaga
48541 gtctgcctgc attaaaatgt tcgataaagc tatttatgac agaaggtgag actttgaaga
48601 gctttggttt gggtggaact aatctgtgcc tcatctacac aggaaagggg aggaatatgc
48661 cagttgctag agagggaata tgtatgtgtg tgcatgtgtg taagcgcatg agagagcgag
48721 agaatgtgtg tttgtgcaca tgcatgtgtg tgtgtgtaag agaaaggagt cagcttggtc
48781 tctgtgtgtg catgtatgta agggcatgaa agagtgagag agagagagag aatgtgtgtg
48841 tgtgtgtgtg tgtgtatgtg tgtgtaagag aaaggagtca gcttggtcta aagggtaact
48901 ccaatttgta catttttcct ttctcccaat catcaataag gttattgaag atgtgtgtgt
48961 gtgtgtgtgt gtgtgtgcat ataagagaaa ggagtcagct tggtctaaag ggtaactcca
49021 acccgtgcat tttccctttc tcccaatcgt caataaggtt actgaaggta caaaatcccg
49081 acttgttttg ttttgtttta ttcattgaag ggactgagct tgaaggccag attggcccaa
49141 gcagaaatgt ctctctgagc tgacataaga gaagagtcaa agaaagagga gaaagctttt
49201 ataagaataa ggaggccttt tgtccaaaaa attggagttt gagctaagtt taattaggca
49261 tctaggttgg gggcttcatg tgacatgtgt gcaactgtga gtgatattat tctttgaaag
49321 aaactggaag agtggagaaa gattccaatc tctattccac ttcatacaag caattccagg
49381 caattcagct actaatcttt ctagggcctt atttcaccta tagattggag ataataacaa
49441 gagccttgta agttatttta gttcaagtga gaggttgtga gttaaataat cttgcattga
49501 ttgcctaata gattcttttc ccttcctcct ccattgtatc ttcactttct cctggactca
49561 agtctctaac ttaaatgcaa acattctatt aattttagtg agcctatcaa ctaataccta
49621 ggtataacct cggggtccag aaacttaata gWtacctgga acagttttta gggtagttag
49681 aaagaaaaaa gaatttcaag gaatgaacag ggattcattc tgaagaagtt cacttagaga
49741 acacaggcca ttcaggaagc tacaaacagg tagggatata aagtttagat cagacagtgg
49801 acaaagataa agatggagaa ctagtcttgg aggacttgta tactattcta agacaatatt
49861 ttcttctaaa gaggccatta aaaatcttct aagtgaggaa gtcacatggt cagatttgct
49921 tttcaaaaag attactctgg gaatagtata aaggcagatc agaaaagtaa agaccagtta
49981 agaggttggt gaccagataa ggagaaaatc ctaagcagtc cctgaagggg aaggagaagc
50041 tgcagaaagt taggaggaaa aactctcagg agcaaagaaa aaggaaatgt caaaggaaaa
50101 tattgttatg gactgaatgt ttgtgtctct ccaatattca tatgctgaaa tctaatcccc
50161 ggtgtcacgg tatttggagg gaaagccctt cggaagtaat taggtcatga gaatggagcc
50221 ctcatgaatt agattcgtgc cattataaga agaggccaga ggttgctctc tttctaccat
50281 gtggatacat gctatgtggg cagtctgcaa ccctgaagat gccctcacca gaacctgact
50341 atgctgacac cctgatctca gacttccagc tcccagaact gtgagaaata aaWgtttgtt
50401 gtttaagcca tcagtttatg gtattttgtt atagcagctt gagctaagac aaatatcaag
50461 gctttgccat tgatattcag tacagaccgt atatacccac aggtttgcgc taagataatt
50521 ttcattccaa acacatattt cagcaacaca agactaaaga gggtgttaaa gaatgctact
50581 gaggaagctt ttgtctggga agttgtttga acaaagttac aggacaattt ttgcaaacag
50641 gtttaaaaaa agttacaatt atgtatgcta tttacgtatt cttgttgtaa ttttaggaat
50701 caattttatt tccctggggg gactgtagga gagcatctgt gtttccatca tatactatca
50761 atcttaggat ataacagtct tttgttcttc acctttgtca attactgcat acagtattcc
50821 ttagagaaaa caaggagttt gaacagatta tctttagcac ctacccagct ctaacattca
50881 tttattatct gatttctgaa attgtactat ctgggttagg tgttggtttt aaggttgttc
50941 attatagtta gcctggatgg ggtcagctat gtttgtagag ggaattccta aactgaccag
51001 ttacttgtca aggatcccat ccaataccat gatcctggaa ttctgtgagt ttagctcaac
51061 tgttcttcag gggtacatgt tttctatttg aaattgaggt atYctcagaa ataaagctat
51121 taataaatag aaagttctgg agtatattta cactcactct gcttatctcc actcctaccc
51181 ctaccatatt cccatccaat actttgccag ttgacttacc aatttcttct taatttctgt
51241 aactgtcacc ttcagtcaaa ttctcatctg tgtatttcta gattcatata atggtctact
51301 actggatctc cttggattca ctttttctca acacagtcct atttataaag tcagaataat
51361 cttccttaaa ccctggtttt attgtttcat tcttttaaga acctgctgca caactctctc
51421 ctacttattg tattaatgtc acacttctgt tcacataggc ctttttcggc tgcttttcaa
51481 tacctttccc ttttatttac atctaagact atcccatcca agactgccca tcttagccaa
51541 tctttccacc tggaaataat ttgctggctt ctgcctgtat gacttttcta atgtcatttt
51601 tatatttgtg tctaggcctt tctataactg tctagaaaat tcctgacatt tacaggcact
51661 cagcaaatat ttgtgagtga ataatcagta agtatgcttg ttttctatca gataagaata
51721 tttatttcca aaYgagattc aaacagtaat actggcctgg gtgtggcttc ctacatgtaa
51781 tcaccctcgt ctatctcttg ttctcagtct tttgctttca aactctttct ctttttttaa
51841 aagagtatta ggaactaaca ttgccaaaat ttggtaatgg tcaagcagat catggtgtct
51901 tactgaagtg aaatattaac attaaaataa aaatgacaag tattatggta tatcattcat
51961 atgtaatgat ccttacaaaa tagagtaaaa ttttaaaaga attcaccaat acaccagtat
52021 gatgtttggg agaaacagat gaatgcacaa aggtgcatct gatgcaaaca cccccagaaa
52081 aatgagcata agttcatatt atcaatattt ttcactctaa aagtgttcac tgacttccct
52141 gaagtcacca aggactccaa gttaagaata ctttcctgag tgaagaaaag aaagcaatta
52201 ttttaatcaa cactgcccca gaatctttta ttcggaatcg ctatctctgc tttcttcttc
52261 ttcttttttt taactttctc ataaatctct gtttagcatt tatatctctc tcactatgag
52321 atacctaagg gttaagaagt aatgccaaag agacatttgt ccagttgaag actgggatta
52381 agaaaaaaca cacagccggg catggtggct catgtctgta atcccagcct tttgggaacc
52441 caaggcaggt ggatcacctg aggtcaagag tttgagacca gcctggccaa catgatgaac
52501 cccatctcta ctaaagaaaa tacaaaacat tagccacgca tggtggcggg tgcctgtaat
52561 cccagctact tgggaggctg aggcaggaga atcacttgaa cccgggaggc ggaggttaca
52621 gtgagccgag attgtgccac tgcactccag cctgggcaat aagagcgaaa ctctgtccca
52681 aaaaaagaga aaaagaaaag aaaaaacaca caaaagattt cccgaaaaga ctaggccctt
52741 ttaaacaggc tatgaacaga gtctcagaaa ggtaatttct tatgtacaat ttttaatctc
52801 attacttgca ttacaagcaa cacatatgag tgataaacta ttttggccta ctggtaaacc
52861 tacatttgtt aactgtgtgt gcattataga gaacaaattt cagaactcaa gagtatatcg
52921 gttatatttg gcttatccag ttgtatatga actggctcca tacacgaata agataaatta
52981 gttttataat tttacaagaa tatctatgga gatttagatt tggctaagaa agttattata
53041 cacacagaag aatccacaac ctataagagc tcttcatggg aaatagtgtg atagatcaga
53101 aagagcatgg acttttgagt cactcagaac ttgaattcca gccttgccat tgttctagtt
53161 gtattatctg aaataagcca tttaaaagac ctgagcacat tgtatacatt gagaattaca
53221 gggttattat gagaaatgca taagaaatgt aaagtaccaa gggctgtatc tgacatatag
53281 aaggtattca atcaatagta ggtatactga accactttgc agagttgttt aaataagtta
53341 attttaaata aggtagttaa tatttgagag tttccttcct tttccacaga ttctcaatcc
53401 taaaacagtt actatttttc catcatcacc atcaaccctc aaatattaac cagttctgag
53461 ccaaatgtga gtgaacaacc tatgatttag aatagctttt agaccaggca cagtggctta
53521 cgcctgtaat cccagcactc tgggaggctg atgcgagcag atcacttgag gccaggagtt
53581 caagaccagc ctagacaaca tggcaaaacc gcatctctac taaaaataca aaaagtagcc
53641 aggtgtgtgt ggcacatgtc tgtagttcca gcttcttggg aggctgaggc aggagaatca
53701 attgaaccct ggaggtggag gagatggtgc cactgcactc cagcctgggc aacagagtga
53761 gactctgtct caaaaacaaa caaacaacaa aatagaatag cttttacaag accacaccaa
53821 atcatgaaaa gtcaactatg taggaagaat atgtatttgt cattggttaa tgacagagca
53881 tcacttacac tgaggttact gggaagatca tttcaaacat ttcccaaaga atgttccaat
53941 caatattgtc cttataagat tctccttact aatgacgttt tgtggtcaaa caagcttggg
54001 aaatattgta tatgtaggct ttttacagtg atttctaatg gacacgaacc taccaaaagc
54061 tagaaggtcc acagcaaaat gagatattta attttattaa caagcattat ctaaacctgt
54121 ttgattttag aatgcatttt tcacacaaca actatcacct cttgaagaat acctggaata
54181 ggttttccta tacctatacc tatatgtcag gtaactccaa aacattaaca aatacatgtg
54241 tttagtaggt tcccatatgg ttgaaatctc cactaacgac attttatttc tcttcctcct
54301 gactttgtat gctatcgaga aatggtcaga gagtaatctt cctccctccc tttcttcttt
54361 tctttctctc tttttccccc tgaaagagaa tagtcaaaaa atccatacat atttattgag
54421 cctctctgat gtgatagttg ctgtgaaata caagaacatg aaagattcag cagcctgggc
54481 ccaggaagga accataataa cctgctgaga aaatcagaca catgatcggc tgctgcctag
54541 agacagtgta aggtagcata taaatcattc agcctgggag tgctactgta gttcgagcag
54601 ggagagggag gtatggacta tagctcagtg tgctgggttt caggaagaag gtgaacttgg
54661 atgtaggtct tcaaggaaga ctgtggtttt aatggctgtg ggaggaggga gtccgctggc
54721 attgttttca ggYgtaggaa atgtctgcag cctgaataaa ggttgtatgt gtggacagta
54781 aatgggctgg gtggagtaga cacattccag ggaggagaag ggatatagag ttgaacactt
54841 aaatgtgtgg ccttgagcta gtggatattg tgggaattgg ataagtgcac tggtagccta
54901 agaggataga atggagaaga gagaaggatg gagactaagg agctattgtg gtagtccaga
54961 tgcagtggaa agcaaagagt tgtggaggta tccactttag tggtctacga tgacagttac
55021 aagcaataga aagagtatga cttgggaaca gtggttcaaa tgctggctct gcaacttagc
55081 aaccatgggg caaatttggg caaattaact tctctgtacc tcagttttct tttttgtaaa
55141 atagggctaa aaatacattc cctagaaggt ggcctccagg ccattttccc acactttatt
55201 tgaatgttaa aactaacttt aatctaaaaa tcatatattc atatctctat cttttttggc
55261 ggttcagttc aacttaaatg tgttatttgt taaaaacttg taattatcat ctattacatg
55321 taaaatataa gagctgcttt taatagtaat tcatctcctc cctatctttt tttaaaacat
55381 ggtcaaaatt aacaaaacaa tcttgtattt ttgatactag tagtggtatc actactacta
55441 gaggtacaag gactcttgta cctctctgaa aagtagcata attagcaaca gtattgatgg
55501 tatctgctat tttagattat ttaaactgta gtcactaagg taagcaaggg gaagaaggca
55561 tgtcatttat gtgtttttca tccttaagat tagcgtttgg aagcatatat tattccacag
55621 caatctggat aggtcttgcc atgccctgat cttgtaagcc caaattttat tttgaatact
55681 ccagctttgc acttaaatga cagctccttg ataaaagaaa aacaattgcc taacaatttt
55741 cttccaattg tctgaaaaca taatgggtgt acatacacac acacacacac acacacacac
55801 acacacacac acacacacaa tctctagggc atatttacta gatagtatta tgtaatgtgt
55861 agacattttt aattatgata attaagacaa aaatatgtgt agcaagtttt tgatttgaga
55921 caaatttgag atttactaaa attattatat gtgtgtcctt ctgaagactt catattttca
55981 ttatatatct tcattcttga agtataactc ttaaattgtt gaattaagtt ttatgaatta
56041 agttgttttg ttttttgttt ttttttgttg ttgttttttt tttttggcca ggcacggtcg
56101 ctcatgcctg taatcccagc actttgggaa gtcgaggtgg gcagatgact tgaggccagg
56161 aatttgagac cagcctggcc atcaaagcaa aaccccatct ctactaaatc taatcttgtg
56221 cctcaagcga ggcacaagac tcacttgaac ctgggaggca gaggttgcag tgagctcaga
56281 tcgtgccact gcactccagc ctcagggaca gagtgagatt ctgtctcaaa agaaaaaaat
56341 ttttttccat ttttaccaat accactattc tcaattcatc cactatttca ctaggaatta
56401 ctaattcagt ttacataatt tattcaatga ataaattatt caatgaagaa gcttctgggt
56461 atttaggtgc tacatgctaa tttcaattgg catattttgt ttttcaaatt tgttcttggt
56521 gacccaataa ttatacctgt ccaccttgaa agtgtctttc ttcatcacct tcaaggccac
56581 cgctaacaag acctttgaaa agaagtaaac agtagagaat ttcaacttct gtgatgacca
56641 ccgtcttctt ctgaatcccc agactccact catacaaggt tttggggaag tttaaatgaa
56701 atgaagcagt ggcttacaaa gtttattgac agtaaactac agtaaaattt atattttata
56761 ctataacctg gtagatttgt ttaaagtaag aggtgctcac ttttgaaggc atgtggatgc
56821 tggctttcag taatataaaa aaaatgtaca aaaaggtttt atacctatat ttatttttaa
56881 aaggtagagg agagcagtgg actcctattt aaagaaactc ttgtgtttta taaataatag
56941 tcatggcaaa atttgtttac agtctttaac aaaccatgca tttcagtata aattaaacca
57001 ataaaaagaa ttatatatat aaaataaaaa ggaaatgtat atataggaac agtatatacc
57061 atacacataa gagtaaatat atattcgagc caatacagag aattgaaaca taatttaaca
57121 tgaaataaca tttatcctta ctatgtatga ttcactctga tgttttctat ttWatttaat
57181 tttaaagtat taattgtgac cccttaaatt gattttgaaa tctaccaatt ggtttgaaaa
57241 cactgaggta agaaattacc ttgcttaata aatgttagtt ttgtttccct cttccctctt
57301 cctccctttt tataattaaa aaactctccc tctacttctc tacttcacgt gagagtagaa
57361 cacataaaaa gatttcagtt aactactgtg catatttttt gaagtaattt ctccataatg
57421 tgtgagtttt ctcagggtac tacaagttta ccaacagaac acataaagca gcggccactc
57481 actgaaatgc ctataggaga cagatagacc atatgaatga atgaatgaca tggttgctgg
57541 gcatcctggt tgcagtggct agaactgtgg agggtaggag cagaccacca tatatcctag
57601 gcatttcatt ggcaaatgaa caggagaagg acacctttcc gtttggccct cattttccta
57661 gatctttcct cccttcctct gtcaatccct cctacatagc ctatgttccc aaagcacccc
57721 ttgtattata ataataatta ttatgatatc tgtccttaat caagaactta tactggattt
57781 ttaatgcctt taggtcttcc ccaaacttca gttcatctat tcgaagtccc tataaaatct
57841 acctccttag acttgtctgc ccccattatt cccctgtggt tgacctcacc tccatctcta
57901 tcttttgtca gcaagtttcc tcagagttcc ctctctgcat acttgttcat gctgggttat
57961 ttgtttttgc cacttgatca tctagacctt tttccctctt tccctccttc tattcaattt
58021 ctgcctttca ctgctgtaca aaggaggggc agggaggccc tccagggtgg agggaatcag
58081 tcttgagata acegagagat tcagggtgat gagtcccaaa gttgaagaga atccacatac
58141 ttcttctgtt tttgttttac ctagcaagga gccttttcta tagacaaagt tRggaaacca
58201 gaggttatga cgtgattcat ctttgcctac ttaaacttta gcttgattca tcacttctca
58261 acaaatactt gttgaacccc accagtaata cgagaagctt ggtattctat gaggtgctca
58321 acttccaaag cttagttcag aagaattctg gcctcaaggc gtctaccatc tagaagcaaa
58381 caactaacaa atgaacaaat gcagtgattt caaaagaaat taaggtgtgc tgtggtagag
58441 gcatgcacag gtcagaaaag ttcagaggtt ccagacaggc ttcctgggga ggatatccat
58501 gccccScctc cacctggtag ttcatgatca gagtttgcaa gcttggctgg gatagaggga
58561 atagcgtgtg aaccacagaa ggagtggatc tggtggctca gacacggcaa gaaccagctc
58621 atggcactag gctgcaggca caccatgcac aaaacagtct tttctttccc cagttctcca
58681 aattctctaa agtggaagcc taggggtgag ggtaagaagg aggtaggaag ctacaaccac
58741 tggagcaaag gacgaaagaa ggggcttgag tgtctaaaag tgcagaaact caaccgactg
58801 gacgctgaga caatgtttaa aaaggagttt ttttgtttgt ttgttttctt gtgttttttt
58861 ttactttaag ttctgggata catgtgcaga acgtgcaggt ttattacata ggtatacatg
58921 tgctatggtg gtttactgca cccatcagcc caaactaggt tttaagaccc gcatgcattc
58981 ggtatttgta ctaatgttct tcctcccctt gctcccccaa cccccaccag gccccagtgt
59041 gtgatgttcc cctccctgtg tccatgtgtt ctcattgttc aactcccact tatgagtgag
59101 aacatgcagt gtttcgtttt ctgttcctgt gttagtttgc tgagaatgat ggcttccagc
59161 ttcattcacg tccctgcaaa ggacataaac tcattctttt ttatggctgc atagtactcc
59221 atggtgtata tgtgccacat taaaaatgac tttttaaagt ggcaactgtg ggaggcaaag
59281 gagaaaaaac aggagctgcc agataaggga gtgaagagaa cccagcactt tgagaattcc
59341 gttacatttt gcagcttgaa ttcttcactt tttgctccca ttatgtgcct ctttctcctc
59401 tccatgaact gggccctgtg gacccaggat cctgctgtct ttgcagggca aagaatggtt
59461 agaaatgaat tcattaaggt gagaaaccat tgctggcata attctccttc acggaaacct
59521 ggcacttaaa tttaacctgt ttggttagat ccagctctgc ctcccccatt tYtttctgca
59581 gaaacaaggc tggcagatcc ctgttcctct taatccttga atggaccaaa tgctgggtta
59641 tgctggggca gtgagggacc ttacaaacac tgaaatttaa gagaacagtt tttggagtaa
59701 ggcagtcctt gtttccaaat ctgagctttg ccactgaYtg actagcttgg caccttataa
59761 aagtgaatta acctagcttg ttttctaatt tataaaatgg agaaagcagt acctatatca
59821 cagtatgatt ctgaaaatta tttaaatagg actatgtatg taaagcagtg cctgaaaaat
59881 agcaagtggt cctaaagtca ctatgatgat gaattttata tgcaggcacc tagagcatgt
59941 taggattttt gggatctttc ccagctcaca ctgaagcttc actcttctcc aacagtctgt
60001 tatccaatgc tggataactg agatagtctt cccagcattt atcaatgtgg ttaggaacag
60061 taatcctgga aagcattctc aaattattta tctaattaaa caagcaaacc tttttagccc
60121 agacatccta aatctctgta gcttgtcttc gctgatttgg tcagaatagc tcagatggta
60181 ggaagggctt tctgggtaga aaaaaataaa ttagcagtct ggaaaacaaa agatctctgc
60241 ttaaaagagt ctctaaaaca gcctgatcag cctgtgtatt ttgccatatt atctagcatt
60301 agtaggggac tgggaaaaat ccagggctcc ttgtagagat agagactggc aaatattatg
60361 tttgatgcat gagaaccaaa tcagaagtta actctttaag ttattaaatc actaaacagt
60421 ctatcacaat tatgtgttta ttactagact gaatttctct ggggctggaa ccataatgaa
60481 ttcatttttt gatctctggt gctaaacact ggctgtcata tactaggtat ccaataaatg
60541 tttgttgaat ggaactgaaa aagcatgaaa cacttcccct gttaaggctt cttttctata
60601 atatttccat agtttctaat gcatctgaga atcattaact ggactgttag aggaatatga
60661 atatggttga gggagctaca aacttcatgc tctgagatgt cacatgatta gtgtaacttt
60721 tgttccaggg gagagaaatc cctaagagag tgactgttct ccatgcccac ccacccacag
60781 tgtcctatag agcctttcat tctccagatg cctataatga ctatcacatt gatttgttta
60841 catatctatt gccttgtctt ccttataggt acagagtcca tacatcctaa ttggcctgga
60901 acttccaagg tctatgcctg ttataccagt gtaattatga atagcattgc ctttcattct
60961 aaaaagtgtt ctggtttgaa tgctaaatca tctagacacc ctacttatga gagggttcac
61021 tgtacatcgt ttagtccccc aactcccctc cccttggaaa tctgggtact accctccacc
61081 gggggctttc cactatggag gcagctctct caatcacagc attcaggagg tgtaagtcca
61141 ctcccaacag aacagacacc tgtctacaaa gagactcttg gtggaagaaa aaaaaaaatc
61201 acttccgtaa gtttaagtct tccttttaaa cttgggtctc ttttgcacat cagaaacaaa
61261 gaatctctaa taattacatc cctaaatgta ctgaagtcta gaagacatgc ccatttgaaa
61321 actctaatct agtaattaaa gcttctcagg ttaaactgca ccttaataat gtagcttatt
61381 ttgtcaagat cagttaaggc cctgtaatgt gatttggaat aaatggacag acagaaatta
61441 aaatgtgctg gacaaggtaa tcagaaatct cacttcattt agtcaataac aatggcaatt
61501 cagcgggttc cttttactac tgggatgtaa ttgtgcttgg ctttaatttc ttgtaatgat
61561 ggaacaaatt aaattacttc atacttttag gaacatttgg ctacatcatg ttttggYgct
61621 tccataaacc ccactggaaa ttaaatcagc tctgtggttg ctcattctcc ctctttagtg
61681 ccatgtctgc cttctgtcag caaaatggta atattgtcta ccttaatcca ccagatgcag
61741 ttgagttaaa agaaattacc tctcccacct ctttcctttc aaatctccct atttgtagct
61801 atcaaatttt ggcatgcctg ctggaacaca gtcttgttag aacaagcacg gtaattctct
61861 cgtgagactt taggactctc tctacgacaa ggtcagaatg tacctgtgat tcaaatttag
61921 atgaggatat gagttggtaa gaacatgaag agcatcagac agttttcagg acacccacta
61981 cttttatttt gtgctatcag tttttccttt cccaaattgc aggtggagat gcaatcttta
62041 acgctggaaa tctcttgcta tatgccatta aaattaatta gtctagcaca ttttatcttt
62101 aggcccttac ctagggcagg taaaatattc tagcacaaaa taacacaact ttcctgaaca
62161 ccatggctac taagttcata tggtaaagat tatactatac attaccttgc aaatagagtg
62221 ttgcagactg atattaaata tttaaaatgt atgcaggatt attaataggc aacattgtgg
62281 cagagactgc taaatgtccc ctaatattga tgcttttctt catcacttgt tttcaaatac
62341 ctgttttcat gtaagcatgt gactgcccaa aatagagaaa attcacagcc tccttggttg
62401 ccaggtgttg ccatataact aagttttggt gaatgagttg gtagtagaag cgttatgtag
62461 atattatatg atgtgctaga aacttccctt aagagaaatt tgtcatctat ctttttatct
62521 gcctgtattt attgttttct ctttcctact ggctagaatg tgaagttgat ggctggagtc
62581 ggagcagcca tattggatgg tgagttgaat ttagcaacag aggctacacc tcacagtgca
62641 ataagatgga aagagcacgt gtccttgaag actgtactgg atagaggtgc catattaacc
62701 tcggactgcc aactttcaga ttttacatga aggagaaata tctgtcttcc ttaagccact
62761 tctattttaa atctctctat ttactgctga ataaaatctc aactaacgca aacaccaata
62821 acaaaaaatt cctattcact catgatatag gattatacct tagtcttttt tgtgtcatat
62881 atctgttcat tcaccatttt tgctttgatg ttcaataaac atctcaaatt taacatatct
62941 ataaaaattt tttaaatttt acgttcaggg gtatatgtgc aggttagtca tataggtaaa
63001 ttgcatgtca tggggtgctg gtgtacagat tatttcacac cgaggtaata agcatagtac
63061 ccctacagct tagtcttaag aagaagtatg ttcacatagg ccaggctgag acttggatgt
63121 tggatgactt tcttccagct tactctgatt tgaatatatt gtgtggtagt agatggctaa
63181 actgcttgct ccaaaactat ctgacaatgc tttttagaaa gtctgccaaa caaaactaca
63241 agaatcaccc tggagcttcc cttaggcttc agatatttgt cttccctgcc ctacttggaa
63301 atcattccag cctggaagtc attgtgagga accaaaaaat ggtctcccta ccatgaagaa
63361 taacactgct tccattgttg ggaggctctc cttcctacag gcactgtccc tcactcatta
63421 gacacttgaa taataaggat gttcatatat ttattggtgt tggtatcagt tagacagttg
63481 aatgatacaa atgttcatat atttcttctt attcaatagt ctcacaagaa cactattctt
63541 gtaaagtcat taaaaactgg atggtctttt cataattatt tccatgattt gtcatctaaa
63601 gccagtgttc tctcctcctg tcaatctgat agctttaaag ctaagttctg ggtgggtcca
63661 agtcatgcct gaaaggttta tcaaatgaca tattgcagtc atatggtcgg agcacaaaca
63721 cctctgaatg tacaatattt taaagcaaaa cagtttatgt gtaagctcta tgaggttacg
63781 gaccatgact atcttattta ttaccaaatc cccagctcct agaacagtgc ccctcaatat
63841 aaatctgtca aatgaataaa cgaagagttt gcctgcagta gtaaacttgt tatttgcaca
63901 cactcgaggc ccacctctat gtgtacattg tcagtgagtc agcctcgtgg atgactctgc
63961 aggactgtct gtataacatS cactacagtt accaggggac ccaattttca taaattgctc
64021 cagcatcaca ccctcctctt acagagactc ttcctgtcca ttctaatccc tgacacgttg
64081 tcatcctcac tcagattccc caatagatag aaatgctgtt aaatatactt gggtagagag
64141 ggataggcat gctcaaactc Sttttgggaa aaaaaattgc aactgtcaga aatgtgttag
64201 ctatcttcac agattatgaa acataaatat caattatctg ttcacagcag tgaaagcctg
64261 catatttttt taagatagca agtataaaac ggctataagt accatgttga gtagagacct
64321 accaccgaag tagagacctt cactgaaata gtgaagggat ggttgtgttg cagggtcaga
64381 atgattaccc actggttgac actccctcca ggactaaagg cagcatcacc agcgtccact
64441 ctgcttctcc actgcttact gttaaggagc aaggcaatag cattatctaa gtaggaacta
64501 cttcattctt tcaaaacctg gaatcggctg atcagaagaa gaaaaacagt ataaaaatac
64561 agaaagacaa gattctctgg ctgtcctaat gaatgctagc tagctataat agcatggata
64621 agacatataa ccaaacatat aagatctatt aattaagggg aatctctatt tgtattaact
64681 catctatttt ctctaaaacc agtatttagt tatctcccat acattggcat attgtgtata
64741 tttgcagaat tcaaaatgtt actgatgtga cttttttcca tcagacatct gagctatccc
64801 tcaactaata gttgagctgt tcctctatag ctcagtatct gagttagaac tcgaagtaaa
64861 aaggtataaa taagtcatgc tataaaattc aaaggcaatc agatgtttga gactatagtt
64921 atacagaaaa gtgtccttgt tcttgggagg aatacatcga ggagtttagg gatagagggc
64981 tatgatgtgt gcagttttct ctcaggtggt tcagagaaaa aaagacaatg tgtgtgtatg
65041 tatatttgtg tgtatgtgca catagagtga tactgcaaat gaggtggaat gtttacaatg
65101 agtgaatgca tgtggttttt tttttttttt tgcactaccc atgaagcttt ccagtgtgtc
65161 tgaaataact tccagataaa acttttttta aaggcaatca gaacagtggc aatgaataat
65221 tcaattattt catatttgag ctgtctttcc tgccaccccc accccctata acactacata
65281 catacatagg ctcacataag tgacacttac tatacttttt tgcctatgta atgagtgttg
65341 gcgaagtacg tgagtgggaa attcagtctt ttgcattcaa gctctaagta attctaagag
65401 accttttaga taatgatttg acttttaaga caacaatttt gaggttataa tatgatgtca
65461 tttcatataa acagaactgc ccttttgtcc tttcagattt gattgaaaat ggcccatgga
65S21 gtacaaccac ctgggaacat atcctcgaga aagacagttg aggcttattg cctaaaccac
65581 aggctactga aaacttggcc aaaggcgact actctagact ggtgacttca aaagtgctga
65641 tgaatacagt gaaccatgaa agatgcatta tttgttttga acattttact atgattgatg
65701 acccagattt ggcaagttag ttcactccca gtggagatga aagtttcact gccttctttc
65761 tttcaacatt cttgttgaga cagatttcac ttttattggc caatgtacta acaaatcact
65821 tgcatttgca gcataatgaa ttcattagca acactgtgga tcaatgctat acataatatt
65881 cctgagtcaa cattaactct taattaaact tccttattat aatgcctctg gaaattttcc
65941 ttcaggctac taaacttcaa gccaacagtc taagtgttaa tcagtaatca tgctagtaac
66001 tttttgagat gggtcagggc cagggccagg gtcagtgatc catcatgaat ggcaggttta
66061 aaaatggata tacgtgatca aaaataccca taagttgacc aaagcattct aacctttggg
66121 ttattctaac actcagtgta tcagccttct gagcaagcca attttttatt gccttaaaaa
66181 gtctaaaaga aagataaaaa cagagataat cagttttgtt tggcaatggc aggggtgtgg
66241 gaaatgaaaa agaagaaagt taaagatgag tgaaaatagg atagtcttgg cactttggta
66301 tgaaattgaa gtacttattt aaactgatga atattgtaca gaaatataca gagagcagtg
66361 aggggctaag aacctaattc caaggtaact acaaggaaaa aattattgat ctggattgtg
66421 accagaattc aagagtttga ctgttgtcat tgttgctgtt gttattaagt taaaaaaaat
66481 gagaaaaaaa tatacttttt tctagtatac ttaccaggaa aggaaagaga gaaggaggga
66541 gggaaggagg gagacatgga gggggcagag ggagagagag acaaagacac tgacaaagtc
66601 tggaaaaaga cacaaattca gatttaagat cccaaaatat gctacatttc tgcccccttc
66661 aaaatattaa actttgctaa aatacactgg aaattttttt tattgtttat tttttaaatc
66721 aactagcaga ccaattagcc aagatgatat tttcatcctt ggtactcttt caggtgagct
66781 ctgtgggcta ccaccagaaa gtcctagcca accaagctct cactgggtca ctctgtgcta
66841 gctagactac atagcctcct aaaaaagcac Rcagccctga tgctggtcct gccaaagtgt
66901 ggtttgctgt catgtcctac aggctacccg tttcccagtg atttatcata ataaaccaaa
66961 tgggaatgaa atacccagaa atatgccata aatctactag tggtgaattt tcaatattct
67021 gcacaccatg gtgatttgtt aattcagttg gatctctatt aaWcctctct aaaatgtcat
67081 taaRccatct gttttgactt gttaatcaaa caacttttaa aaatgtattt agagtatcag
67141 taacccttga attaatgcat ccatcagtaa taatcccctc tccaacttta gatttatttt
67201 taagctctag cttttttccc caccattcca acaaatagcc tgacttccaa aagtaagaat
67261 caatcacttc agttgtagaa atgtccagag ggtgccctac tttcgaaaca ggaagtaact
67321 tgatccagca gtcccacttc ttggtatata tccaaaagaa ctgaaatctg gatcttaaag
67381 agatattttc actgcagcat atttgtaata gctaagatac agaggcaacc taaatgtcca
67441 tcaacagatg aatggacaaa ggaaatgtga tatataYata cacacacaca cacacacaca
67501 cacacacaca cacacacaca cacacacaca cacacatata caatgaaata ttattcagcc
67561 ctaaaaaagg aaggaaatcc tgtcatttac aacaagttgg atggaactgg aggacatcat
67621 gctaagtgga ataaaccaga cacagaagga caaatactgc atgatctcac ttacatgtat
67681 aatccaaaat agtcaaactg atggaagcag aaagtagaat ggtagttgcc agaggctggg
67741 ggaagatggg aattggtaga tgtggtcaag ggttacaaag ttttagtctt gcaaaataag
67801 ttctggaaag ctgctgtata acataagtat acaatcaaca atactatatt gtatacttag
67861 aaatttggta aaagggtagc tgttatatta agtgtcctta ccatgaaagg aaaacaaaac
67921 aaaaaaaccc aaaaagatgg gaggaaactt ttggaggcaa tgaataagtt tatggcattg
67981 attgtaatgg tggttttagg gtgtatacct gtctccaaac atcttgcaga ataaacatat
68041 taaatctaaa gtttccttgg gaaagaagat gagaaaataa ttgtcaagag actgtacacc
68101 aataaaatag tcaaggagta caatgaaaac ttaggaatcc catttcttcc tttgactcac
68161 actgtttgat gatatcattc acatcggcat ttggaagtta ttgagacatt tcaacataag
68221 attgtcctta tgatctatga tttgggactc tcttataaga aagtccattc tataccaatt
68281 tgttttgttc cttggtaact agaccttctt taattggatc taagaattaa tattcctatc
68341 tcataaagaa gactaaaagg atccaagtag atggagtttc aaaggtggtc cccataaaag
68401 catttttacc ttaagcaaag atgtcccaat ttttcctgct tgcttttcgg atgctgttct
68461 aaaaaaaatg taccttattc cttctactaa aagaaaacct atcaattcat tgattcattg
68521 aacaaatatt tattgaacat gtagtatgta ctaaacactg ggtaagattc cagatatgca
68581 aagaggaata agtccctgcc cttaagaaaa ctcacagtgt ggtcaagcta acaattctag
68641 agcaactgtg tgataagggc tccaaaagca catgcaggaa cacagagaga ggcactctta
68701 gtcttggagg ggcttcctac agtgggcaat ttttgatgtg tcacaaaagg aggagctatt
68761 agggcacaag aagagcaagg gtgaagtgca gaaaggagtg aatagatggg gaggggaccc
68821 aggcaaagag aataacatca gcaaaggtgc aacttcatgg gaacacagta catttggaag
68881 cctgcccacc atttgttaca gcagaatgca tgtgtgggtg gagatgaggc tgcagagatg
68941 ggctggagag gattatgaga gatggatgac tagcaaagga agttggtcat gtccttatgt
69001 ggtgggggac catggaaaga tttttattta taagacgtgg tttattcttc attctgtggc
69061 aaaaagcaga tactatagtc tgaatgtttg tgtccctccc cacattcata tgcgatgaaa
69121 cctcatcacc aatgtgatgg tattaggagg tggggccttt gggagataat taggtcatga
69181 gagcacagcc cttataaggg gattagtggc ttataaagga ggcccaagag agctgccttg
69241 ccccttccac catgtgagga caaagccaga ggcactatct aYgacccagg aaatgagccc
69301 tcacaaaata ccaagtctac cgtgccttga ccttggacat tctagtctcc agaactgtga
69361 gaaataaatt tatgttgttt ataagccacc cagctcgtga tttatttatt tatttattta
69421 tttatttatt tatttattta tttagagaga gagttttgct ctgtcactcg ggctggattt
69481 cagtgccatg atctcagatc actgcaccct ctccttccca ggtgcaagtg attctagtgc
69541 cttagcctcc tgagttgctg ggattacagg tgcttgtgac cacatttggc tgattttttt
69601 gtatttttag tagagattag gtttcatcat gttgcccagg ctggtctgaa actcctgact
69661 tcaagtgatc tgcccacctc ggcctcccaa agtgctggga ttacaggcat gagccactgt
69721 gcctggctat gatattttgt tacagcagct tgaatggact aagacagaag gaaaaatagg
69781 cctttctgta gcgtgagctg aaagacagaa aatagccata ggtttctaaa gagagaaaaa
69841 gatatatgtt tgacaatatg tttcaattag tacttttttt gaaatgcagt aagttaccta
69901 taccttattc catttctaat ctatatgatg gcatttaaat attgactagg tttctgatac
69961 agtttttgga gtctgaatgt cactgcatgg atgaataaac caaacagatc ggattcatat
70021 gggtttgcca aatgacaaat ttttttccat gtaagattag aggaaaaact gatttcaccc
70081 aatttcctgc cttatttata ttagaactga agtcaatgct gttatccctt tgcatgttct
70141 tttgtattta aaatctgata tagaattgtg ctgttacaga agaaataatt taatgcattc
70201 tgccctctta tttctcatga gttttcattt aaaagaaaac aaaaaagaaa aaggacatgg
70261 acttttcttt atttctagac tttataaact ttttagttat caaataaaca catgtattaa
70321 ttaagtaggt aaaatattgc atatattaaa aggtacttgg agWctcatat gttgtcagtt
70381 tttcatgact caaaatttat gaaaaaccat ctaagactat cgtaatgaag ttcaattgta
70441 gatcttgaag tgacaaaaca tgaaccacat tgacttgagg tccagggctt aaaactgcct
70501 atttgagctc taggttaaaa gcatgtgtga aatgcaaagt ctgacatctg cacagctgac
70561 tattgtgaag tctcagtatc attgttcata agtaaagctt gtccctaagc cataccacaa
70621 gcattcccag aggatataga tttcatYtga tttaacatga ttgttgatat tggtagtgta
70681 tttcacgtga acttgtgaaa tcattgtcta tacagacaat tgagacacta aaactttttt
70741 ttaagtattt tttgttaggg gctagatctg aggtttttta ttcatttgtt cttttgttca
70801 ctcatttgtt agttcagtta tttactcaaa aatgtatatt gagcaattcc tatatggcag
70861 acactgacag atgctggatt cagtgatgag caaagcattc gtggtttcta tgcccaagga
70921 tcctatcccg tggagtgtag agtcaagcaa agaatgtcaa caacaaatga aaattttaat
70981 cttattaatg ttatatgcaa aaatgtaaca cctccaaaaa agacaattta tttcctatta
71041 aggaatgaag gcagtccact gaggaagatc aacatgtaag cacatgtaaa gcaaaataat
71101 caataggtaa tgtagttttt gaaaaagaag gggctttaag caggaagtcc tgggtttgga
71161 cttgggtttc aaatctagct atgtactctt gggccacata cccaagtctt gtaagtctca
71221 gtttcctcct ttttaaagtt agcctaaaac tacctcgctc atctaatggt gtgagaatta
71281 ggtaaacagc caggtagctc atcaaattgt aattattata attgttattt tttgagtcat
71341 gaaagttttc acactgaagg gaacatttca acttgacctg gaagagtaag tagtttatca
71401 ggcagaaaga atttgttagg gtattccaga gagaaggaac aaaatgtgca aattcaagta
71461 gtcagaatca aaatggtttc ttaaaggaat gacaaacaga tcagtgcagt tggagcctaa
71521 agttatctaa ataatccgtt agaccttttc tttaactgat aaaataaagc aagcattcca
71581 aaaatcaatt taaatccatc ttcctcctta tacctctcca ttccccatac catggaaaca
71641 cagttttacc tcttttacaa tgagttgtca gcatgaagag acttggttca ttaagattag
71701 caaacttcac agcattgaac aaaagataaa gaaaagttta tagcaaatac gttccatgtg
71761 aaagcatctc cagcaggttc agaagatgca atgaaaagac taaaacttga tatagggata
71821 tttcagtggt gctYgtacca gcgtcctaac attttgttct accttagtaa aatctatccc
71881 ttattactca tcctatctgt agactctctt atggaacaag aagctttttt ttttttttcc
71941 ctaatcccgt ggggaagccc tctccttccc tgggtgattc ctgagcaacc aggaaaacag
72001 cagtcacagt caacacagct agtacttgca gaaaatctct cttctgtgat gctctgaatg
72061 actcacagtt ccagatggcc ttgtcagcct ttatccgcat tccactcttg gcaccattaa
72121 cctggtctaS tgtggttgca acaaataact tttcttgtta tgaggaccag tgttttgaaa
72181 aatttaaaat gttataaaat taaaatgttt atatttggaa attatttcca aatataaaag
72241 gagcctgtct tcattataga ttatttggaa aaaatgcaga ggggtgcaaa agaaaaatgg
72301 aaacaagcca tctttttttt acccataatt ctaccactca gagacaacta ctgtgaacat
72361 tttgatgtat ttatctccag tctttctaga aggtagatca ctgtaagaga tttctgaagc
72421 agaagtacta agtcagaagg aataaacatt tttatacctc ttaatgtgta ttgccagata
72481 gtttttgaga aatattacag caatttctac ttttagaagt actgcttgag agtgatgtta
72541 catggtattc tcactaacac atatacaatg ttataaatat gtccaaaaat aaaacttagc
72601 aactatgtta tctccttgtt tcaatttgaa cttattacaa gagtgatcaa tttttaaatt
72661 taaatgttat ttttagcaaa gttatagatg caaatagttt agagaactaa gtagtaccac
72721 aaggattaaa aaaagagatc tcctgcagtg catcccccac ccacaataca tacatgtatc
72781 ttccactccc cagagggtaa ccattttcaa atattttagc tgattcattt gctatactcc
72841 actctatctc taaatgatat aactttacag ttacttctta atagttcatt tttttgggca
72901 ttgcttattg atacttcata gcggaagata aggatttacc tttgcaacac ttcacacttc
72961 tcatcaccca tccttctaat atattatagt cttacttaga ttgatagtca ttgtgtttcc
73021 atgagcagaa ccaaagaaaa actataagca gctaaactat gtaacatacc ctgattacct
73081 ttttttcttt gttttccttc ttgaaaattc ttgtcttttg ttagtttctt tgttccctta
73141 gatgtccata tatacttatc actaattcaa tcccaaactt ttcccatatt acaaaaatct
73201 aagtatggct caaacacatc aagaatttta ttaaattcat cttcttgaag aaatctcatc
73261 cagaggcctc tttgacctgg attggttatt ctccatgtct cttgctcagc tgtcactttg
73321 ggatgttctt tccttattat cccaagggta atgtttttag agtttattcc cttgttttgg
73381 tgagcacgtt ttccagtagc ttcctgagtc agtatgcatc agagatacat tttttaaagg
73441 ctcgcacctc agaaataatc tttattccat gctcactctt gatcaatagt ttggYtgagt
73501 atagaattct tggttgagaa tcactttcct tcggaattta aaaggcatca cttcattctt
73561 ttctaggttt caaaacgaat cttcagaagt ctgatgccct tttgattttt gatccttcta
73621 tacgatacga taatgatcct tagaacaagt cacaagtctc ttttttaata ctttatattg
73681 tgctaaatgc tccaattaat gagttctgga aatttttctt caattttgtc ctcctgcagt
73741 tttctctttt ttctagaatt tctgatttta gatgttagat ttcctgaact ggtcctctga
73801 tttcccactt tttcttctgt attttctacc tttttgtatt tttgctccat ttccatagag
73861 atgtcctcaa tttacctttc aacccttcta ttgagttttt aacttattct atcatgtttt
73921 tactttccaa gagctctttt cctattctct gagttgtttt ttttttttcc atagcatctg
73981 cttttgtttc gtggaagcaa tgttaatatt tatgaataca atatcaagtg tatttcttct
74041 cctctctctg atgatacctt cttcctgcaa catctgtact tttttatttc tcttcttttt
74101 tttttttttt ttttttgaga tggagtctca ctctgtcacc caggctggag tgcagtggga
74161 ccatgttggc tcactgcaac ctccatctcc tgggttcaag caattctcct gcctcagcct
74221 cctgagtagc tgggattaca gttgcctact accacgccca gctaattttt ttatattttt
74281 agtagagacg gggtttcacc atattggcca ggctggtctc gaactcctga cctaaggtaa
74341 tccccccgcc ttggcctccc gaagtgttgg gattacaggc gtgagccacc gcgcccggcc
74401 aacatctgta ctttgaagtt ggtctttttc catttgtttt ggtttctttc acttactttt
74461 tctaagtatt tgataaatct tatctgtcta ctcatattta aggctagagt actaaaaatc
74521 agattggaag ctctgtttgc aYaggtaaga tttgtcaact acatccactt tttgatgatc
74581 caactgaaca gtttcattca gaaatacctt gcaccattat ctttgggttt tttctcttgg
74641 gttggtgaat tctccaaagg ttatatcaat ctcctgtcca gaaggcttaa gcatagttgg
74701 aagctgacta ggggaagaag gttgggactt tcaaccttcg gaattcattc aacaaatatt
74761 tgagcatcta tatttggcag cctctattct agccaccgga gacacagtgg taaacagaat
74821 ttaaaaagcc ttgtgttatc atgaacacaa atatataagt gtttatttaa tccctctgtt
74881 ttcagaatgg cactcagtct tcaacctgcc taggcccccc ttgtctaggg acggttttac
74941 attcatcagg aagtataccc cttgtatact tgtttttcag cttccaaaat tttgttgctg
75001 ttatctccct tcccactttc tttgtccttg tcatttatgc ctataaacat ccattgatgg
75061 ttttctgaag gcagaggagt taaatggata tgttaattac tatagatgta atcagaggtc
75121 ttcatgttgt ttttttgttt tctgttttac tattctaact tggcatttat agatcttctt
75181 tgtgagtagt ctgaatttgt ctttaatgat ttaacattga aacagtattt tccttactta
75241 tttgcataag ctctttcttt gttagtgtat tagccattgR cctgcaatat cttttaggat
75301 gaccgattct gaagcctgct gactgagttg cttgtggttc ctgtcattta gaatgtatgc
75361 aggtcatgaa tcgcttagat ggaacagatg gaatgaaact tgctctaaat attttaccaa
75421 aataaataca tttaaataat gtaaagaaaa atcttcaatc actaggcttt gaacttataa
75481 cagaaaggta tctgctttta actatattaa ttcaacaaac tttattgaac agttggtaag
75541 tatgaagcat catctctctg agaacaaaat accaaggaac tgagtcccat cattacagga
75601 ttctcaggag taaatgggtc aatacatagt tataaaatga tgagggctga tctatagcag
75661 aagtacaaag ctgagaacaa ctcacttgac ctgctaagct ggggaattca ggaagggttc
75721 agatctaaag agcagacatt tgaggtggtt cttgaaatat gagcacaatg atggttttac
75781 caggctgcaa aggtgacaaa gtgcattcca ggctgataaa agagggcatg caaaatatgg
75841 caggtacaaa aatgtgtatt taaggaagaa tgagaagttt ctggaagttg ctgaagatac
75901 agaaaataaa gtggggggcg gggggggcgg gtggttggag atgaggagga gatggtaggc
75961 ttgattcaaa ttatggaaat gtatttataa tgcaccaact tgtaaagaca ctgttgttcc
76021 ctacccagca gccactagaa tttgttcagg tgtcaggcgg ttggtgcttc aagggagatc
76081 aggcccctcc caggaccagg atgaaccatg attacttccc agccaatttc atagcatttc
76141 tcttgccaat gaatggctta gatataagca tatgatgaat ttgtttctaa tacagatctg
76201 agaaagaaat ctgttaagga acttctggta aagtttttat ctttcttaaa aagtgacaga
76261 agaaaaggaa gttgtataaa tatacttaga gctgctgtag tcatcttgag accataagag
76321 gataaaactg agatcaaaaa ctactgtgct gaggatgggt gactagaaag aaggaaagaa
76381 tctggattct tgatgcatgt ttgtactact agaatcattc tatcactacc ttcttattat
76441 ataattcaat aaatctctta ttatttaagc tattttaaat gaagccttct gttatatgCa
76501 gcctaaacaa atatactgat tcaagctccc agcaaattct gtcacacata attcaaggaa
76561 cgtttgttaa taacttcata ctattatatg gggaataaat gattagtcaa gaaagacttt
76621 ctgttttgaa aaattctgga aagatgataa tacagtatgt agatttattg tcatggctaa
76681 tatacatcta catataacat gaattacctt ggagtagtaa taaaagggca gagtaataac
76741 ttgtgcagag ccctagacta gaaaagttga aaatataaaa tggaggaaaa agtgagttat
76801 gagattcagt aatatgaaag tataagaaat aaaagaatcc aaaagccata gaaggttgaa
76861 actgaaaaga attttagaaa gcgtctaggc caggtgtggt ggctcacacc tgtaatccta
76921 gcactttggg aggccgaggc aggcagattg cttgaggcca ggagttccag accagcctgg
76981 ccaacatggt gaaacctcat ctctactaaa atacgaaaac ataactaggc gtggtggtgc
77041 atgcctgtaa tcccagctac ttaggtggct gaggtggaag gattgcttca acctagggag
77101 gcagaggttg tagtgagcca agatcgtacc acttcattcc agcctgggtg acagagtgag
77161 acactgtttc aaaaaataaa aagagagtct agcctaaacc ccttaattat acagattgaa
77221 aaccagtcct ggagaaattg gaagtcctca aagagtttac cattaatggt ggaaccagga
77281 cttccacaca atgttctgac ttcctttaga gcacttttta ttatatcata tagttcctta
77341 cttctcacta ttaatagaga ttattttact aagagtaagt ataataagag ctgatcagaa
77401 gacctgagag acataatgga gtttcaattc taatttgctg ttggctcctt gaaatgcttt
77461 gttgagaagg agtaaaaagc ttacaaaaca cacacacaca cacacacaca cacacacaca
77521 cacacacaca cacacaattt cttaattatg tctaccatgg gcatgggatg gtaggaatgc
77581 catgtaacta gtttccatgg atggaaacca cctaatggga aatagagaaa aagaaaaaga
77641 aaagaaattg tgtgtaaatt ttcagctcat tttggcttag attacatgat gaaacttatt
77701 tctttgaaaa actcatttga ttttgaatta gattatatgg aacttctcct ttgtcaattt
77761 taatttcttg tcttgtttta gtttgctttt ctgagcagta gatatcctcc caattggaga
77821 ttccgcaaaa ttagtactta gtcatcttgg ttcctttcaa gtagaaaagt ttcttttaaa
77881 tacttcaaat cattggtatg cacatttgtt gcaaaaaaaa acctcattct ggaaaatgtt
77941 gcttatttta ttatccaaaa aataggtatt tattaatata ctgcatttct tttaaataaa
78001 ataaattaca gattttcttc cagcatccac ttatttggaa ttgggtacat atgaagaaaa
78061 gacttcttat aatatggaat tatttcaaaa gctattatta cttcttataa atataaagca
78121 tacaagaaat tctcaccatt ttctaagatt tacattagaa agatataagt attgtctttt
78181 agaatactgt ttttattttt ctaaatattc cttataactt acttattaat tttatccttt
78241 ctttgttata aatatccatt ttttttaaag acccatacta tcttttccta tgcaatttaa
78301 cctaacatat tttctgtaaa tgaggttatt tagttatcca tttttctacc tcctgttgct
78351 tggcagtcct caaaaccatt gcagatcttt ctagagagca gtttttaaga acagtgcttt
78421 acttttcaac tcttgatcaa cctgatgaga attctttgaa taaaccagaa ggaaggctaa
78481 agagacaaaa gaatactata gtttccttaa agttaatgag ccagaatcct ttggcccact
78541 ttggcattga acaatgtgaa taaaatggcc actctcacat cagtaattta cattttatac
78601 agtgtgtact ttacactgat atttacaaag aggaaaaaca acagtttttt acatgtaaga
78661 ggagctcctt ggcagataaa agaaaaactt actcccatta gtacaacact ggagtttaaa
78721 tctagagaac aagtacttca gaacctagta gaaaaagtcc ctgatttgta ctttgtatga
78781 acaattaggt ttgagaatat tgctatataa tcacagaaaa tactggctat tatactacgt
78841 gaagtcttca gtgacaaaga gagaaacaga aaaaatagtt aatgccataa caatgaaaat
78901 gtggaatgtg aggaacgccc agtgaccgta aatatctacc ttaataagcc tgacaatttg
78961 tatacagttc atgcaattaa caagtgtgat aaatatgatt attactctca agttgaaatc
79021 aattttcttg gctaaaagca aagaattgtt attgagcaga tgggattact tttctttgcc
79081 aatttatctt cataaaactc aatcaatgcc ttgccttaaa aatgtcaaat aactcagatt
79141 tcaggataac acatacaaac taatctcttc tgcctgtctc tacctgatca ctccctactt
79201 caaaagctcc aacaacatag tataactgac acaaatcaaa gcaaaaacta tttttctcac
79261 tgctacatgg tcttatcaat aatccagtat ttcaatatct aataaaagat accacaattc
79321 aaagcagtaa tgtgccccaa gctaccgatc cacaacatat atattgatat ctaaaaatat
79381 atggatataa cttgttatct actaaataag tttctttgca gtgtggtgtt aatagccaga
79441 attttaaata cataaaaact tgggttcaaa tcctaactct gccacttaaa tgctatatga
79501 caattggtca agttacttaa cctctctctg gtctcaagtt tcttgtctgt aaagctgtaa
79561 tagcttttag agtgctcttc tgaatattaa aaaggcatta tactcttagc ataatgtcta
79621 catagagtaa atatttaaca acaaaataac aaatattagg tgtgttattg gagaattgga
79681 gttttaaata aaggagttgt ccctaaggta cattcaattt aactatgata tttaaaactt
79741 caatttttca cattattttt ccaggaaaat atttcactct tatttctcca taatttgttt
79801 tgaattgtaa aagagatttg caagcggctg aagcagttac actatactgt atatctgcag
79861 gatgggcaac tgattttttt tttcttcttt tttttttttt tttgagacag agtctcactc
79921 tgttgcccag actggagtgc agtggtgcga tctcgctgca acctctgcct ctcaggttct
79981 agggattctc tgcatcagcc ccctgagtag ctgggactgc aggcacacgc caccacgcct
80041 ggctaatttt tgtattgatt ttcaagattt gtcaagagct cctaatattt tcttttctca
80101 aacacatctt aggactacca tgcatatcag tcagtcagct actctggagg tctgctgcaa
80161 ggatacacac cttccattcc cacccatcag atacttgtct cctttctccc ccactccaag
80221 acagcaacct tccagctcta gataaaaaga gctagtcttt cttgcataag aacaacaaag
80281 acaaaaacca ttttggtagt gtatactgca gattttaacc aaaacttctc tgaagacctc
80341 tgatttccta aaaaacctct tcatcccagt gcagttgctc tttcctaaat gagcaaccgc
80401 gcatctggtc aaatttggtg aatattctgt gctccagaaa caactgattc catccccttt
80461 aatctcttct tctcttatat ccatctccct ccttgttgtt tctctccatg tttacaacta
80521 gttcattgat ttaaaaacca atgtaataca caaggaagta tgcacgacaa tgtttagtgt
80581 agtatggttt gcaatattaa aaaaggccat cagtaagaaa atagttaaag gataaacttg
80641 atatacatac tatgctgcaa tttaagaaaa ctggtataga tttatacgta ctaaaataaa
80701 ataatctcca aaacatagta agtgcaaaga tcaatcacaR aataacatct gtagtatatc
80761 acttttgatt tttaaaaatt acccctaatt ctaaatgttc atatatataa atattcagtt
80821 aaaaattctg aaaagatact gctatggttt gaatgtttgt tccatccaaa actcaggctg
80881 aagtttaatt gccattgtaa cagtattaag aggtaggact cttttttttt tttttttggt
80941 gacagggtct cacttcaaca cccaggttag agtgcagtgg catgatcata gctcactgtg
81001 gcctcagctt cccaagctca ggtgattctc ccacctcagc ttccagagta gctggaacta
81061 caggcacatg ccaccatgcc cagctatttt gttgtatttt tttgtattga tagggtttct
81121 tcatgttgct caggcaggtt ggtctcgaac tccttacctc aagtgatcca cccacctcag
81181 cctcccaaag tgctgagatt acaggtgtga gccaccacat ccagcaagag ttgggacttc
81241 taagaggtgt taggtcatgt gagctttgcc ctgatgtaag ggttaatcct gttatcactg
81301 gagcaggttt gttataaaag agtgagacgt ttggtcccct ttcctctctc tctcccaatc
81361 tgtctatctc tctccctttt gctttctgcc atgtggtgat gcaacaaaag gacccttaac
81421 agatgccagc accttgattt tagatttccc agcctccaga actgtgagcc aataaatttc
81481 agctcatgat aaattaccta gtctcaagta ttctgttaga gcagcacaaa atggattaag
81541 gcagatatac accatattgt atccatcact aagtggatac tttcaagaaa gacagcaaga
81601 ctaaggagca tgaaaaagga ctttctcata ttctgaattt taaaaatatt ttatgacatg
81661 aatatattta tctattacct atgtaataaa actagacatt aagagaaaaa tctcatggta
81721 gaaatcttag gaaatagaga aaaattagag aaaattaaga aaaagcatgc ataatttcac
81781 aaaccaatgg tagccactgt aaaaaaatgt tatttcattc taatacattt ctacacacat
81841 atgcacacag tgcatttttg aaaacataac tgagtctttt ctaattgacc tcttactcta
81901 caaaatttga aatagccaaa acaaattttt gattcctata aagagatagc actagagctg
81961 gaagagatgg ctcatgcctg taatccctac actttgcatg actgaggcca gagaattgct
82021 tgaggccagg agtttgagac cagcctgggc aacatagcag gacccatctc tacaaaatta
82081 ataataataa taataataat aataagctgg gcataatggt gtgtgcctat tgtccttgct
82141 acccagaggc tgaggcagga ggactggttg agccctggaa gttcaaggct gcagtaagct
82201 atgattgcat tacactctac ccagtgtcaa ggaaaggaaa ggaaaggagg ggagaggagg
82261 ggagaggaga ggagaggaga ggagaggaga ggagaagggg aatgggcagg ggcaggggca
82321 aggcaaggaa aggggcaggg gcagaggcaa gggaagggga aagggcacta ggacctatga
82381 gccaacatag gctatggctt ccactagtac aacttaaaac tcagctgaag acatgacaca
82441 tagaaaaggg ttgctgtgtc tgtacacaca cacgtttccc atgttcccaa tcccagtctg
82501 tggtcagtga aagtgaagat tgcctcagag cctgcattat ctcaagctac tgacctaatt
82561 gctaatgctt tactctggYc tatttcctgc Mtgacctcag aatgtgtcag tgtaaattcc
82621 atagtaagac agtagttgca cattttggag tgtggattct gctttgaaac acaaataagt
82681 gtgatgaacg tgaccctggc actctgtagc cctcagtttc ctcaacgcta aattgatcac
82741 tacagttcct tctagtgtaa acagtcccac gaagaatcgt gctgaatgga tgactacata
82801 atggcctgct cctagagttc ttaaatcaat ccttaaaact ggctgcaaaa tgacttcact
82861 cctttaacag tgtgggactt gtctctgttc tccagaatgc tcaccagatg gtgaagaagt
82921 atctctctac ctaccaacat cgtcatattg cttcttgcca ctcgctactc aaaaaYtgtg
82981 aaaaacctgt gggagcaagt atcagctgaa aagggtggct ttctccttag gcaaaagctR
83041 cagggcaaaa cgggggcccc aaacttctat cacgtcaaag acatgaaaca gatgatccct
83101 agttttgatc ttctttgaag tttattaaaa acaagcttga atctgtacca atcctgacag
83161 gtgtgaatgt aagactcaca aatgagaaat gctaagcttt cctaatgttc aatttaatct
83221 tatagaccat gaaacatgtt ataattaaag atctgaacta ttaatatgaa ggagatagca
83281 tatattctta ccccaaatat gactaactag gtaaaatctt caaaatataa tcatggcctg
83341 attatcagcc attgtggagg agaattttaa aaagttcttc attgacatcc caagtttatg
83401 ttttagacac ttctaatcaa acatgaatag attaacttca agtaaaaatt aatagtcaca
83461 ttattgatta tcatgattgg aaatgtattt gaccagaaca atgaggaaag agcatagagt
83521 cctgctatct tcaagtatga atttacactt ttaactgatg tcaagtcatc catagtatga
83581 aaactcattt atttttattt gaaaaattta aaaccatcaa aatggatagt gtgggagcca
83641 aataatatat aagcaatatt ttatgagaac tacaaaacta ttaagagcag taactagaat
83701 tcggttactg ccgttcctta tttcagaaga gaaagtcaaa tgttttctga tatttaagaa
83761 gtaataactt cctgttgtto agttatgctt tctataatta tatatgataa gcaacagaaa
83821 aataggtctg gttttggctg taatgtgtat attcaattca atattaattt ggactttaaa
83881 tcattttgct atcaaatatt ttgaacctca tgaccaggaa gtaatcaggt aaaaaaaaaa
83941 aagaaaaaaa gtgggtttgt tacctgacct atctcttaag ttcaaacttt ctataggtat
84001 ttttctctac ctttgtccat atggttatac aattatcact agatgcttga attactaaat
84061 ggttttgtat atcttttcct caaatttata tttgtcctat tattggctta gtctctcaaa
84121 gtattacttt tatgatattg ctataatggt cctctatagt ataaagttga gcccacacca
84181 tttatcctgc cacttaacaa tgtccttaat gtagattata tggtttggcc atgccctaac
84241 caaaatctca tcttgaattg taatccccat aatccccatg tgttgcggga gggactgggt
84301 gaagataatt gaatcatagg gcagtttccc ccatgctgtt ctcatgataa tgaatgagtt
84361 tcacaagatc tgatggttat ataagcttct ggaattttcc ctgctggtgc ttactctctc
84421 ctgctgccct gtaaagaggt gcattctgcc atgattataa gtttcctgag gtctccccag
84481 ccatgcagaa ctgtgagtca attaaacctc ttttctttat aaattaccca gtctagggta
84541 tttcttcaca gcagcatgag aatggcctaa tacagtagta tatcacactt attaaagagt
84601 tgatagtaaa atagcactct cagtacaata agaatagaat agttcctttt catggtaatg
84661 aatagctttt cttcgacatc tataattaac aatgaaatca gaaacaaaaa gaagctgtct
84721 ccactattac tgaatgttgc tcagaaaatt ctaacgtaag caaccaaaca agaaaaccaa
84781 atatgctgta caaatatttt taagagagaa ggaaatcatt ctattttaag ataaaaattc
84841 tgtaactcaa aattcaggag aatcaactga aaggtataaa aaaggtagac cattatgatt
84901 tataagaaag gtcaaacatt ataaagcatg tatccacaac aggttttttt ctatatacca
84961 gttagaaaat agataaatga tcctattcat aatagtaata aaacatatca aatatgtata
85021 aataaacaac gaaaaatgta aaactcttgt ataaatatta ttgcaaaaca tcactgaagg
85081 acatttttaa aaacttgaag aatatatcta gtttcctaca tgggaagagt caatattata
85141 aagatatcaa gtcttagatt aatctatgag ttaaatgcag ttacttttgc cctaaaatgt
85201 taaaaggctc ttaattatgc aattcgaaat gtgatgtcta ctttcttctt gaaagcattt
85261 ttcctggaac cttctgatga ctctaactta ggctgcctgc tttctaggcc tactactaca
85321 cagttgtcat actgggattt attttcataa acatctgggt tggttccaat gctctttgta
85381 tcctaggttt tctgtatagg attcccgttg ctttgttttg cttgaatata tcttcgagta
85441 atacttcctt ttaattagga agaatctgta ggaggtaaag ttgtaaactt gagtccttcc
85501 atgtttaaat acatctttag tttgtcttaa cacttgatag tttgactatc aattttgggt
85561 tctagttctt cttcctgaga actttgaaac cttgcctagt ctgcattcac ttctaatctt
85621 gctgatgaga agtttgatac ctttgaaaga tccttttttt ttgttctctg gaaggattaa
85681 tttcttatac ttagaattcc aaaattttca tcagcatgtg tatacacatc tagatatgta
85741 tccttttttc cttgttcggt gttggatgaa acttttcaat ctgaggactt gaattactct
85801 tcaggtcaag gatttttttt ctgttatttt cccacatcct ttcccattcc tccacttcta
85861 ccacattctt catatctttt ttacttttcc atcRtatttt atatccttgg ctttttgctc
85921 tatattctaa gatatttgct taattcaata ttcaagaatg aatattactt gaattgcatt
85981 ttatccaatc tttatatata catatatatt cagtattctN ctatttatgg aaatcagatc
86041 atacttaatg gttatattct ccccctgaat atctctgaaa atttaatatt gacaaatttt
86101 tcccacactt ttcattgtct ctgtttcttc tggaaagcag aagataaatt gtctcaggaa
86161 aataaatttg tcctgtttat ttattagttc ctttttgttt aggctactgg tttttttgtt
86221 tggtttggtt ttttgtcttt tttcaaatgt ctggtgatcc tttcttgact gggaatattt
86281 gtaatttaag aactaggagt ttcttaagta gttggcaggg atatcataag gagctctgta
86341 gtacttataa actgcttata agcaacttaa tgcaatttca atcaaaatct taatgggttt
86401 ttttgggggg gacttgacaa gtagattgtt tgtataattt agcacaacga tgtgagaata
86461 acccaggaaa ttctaaaaaa taagattaag agggaatatg ttctcccaga tattaaaatg
86521 tatttcttaa aatccataat aattataaca tggggctttt tgtctagaaa taggatgtaa
86581 agattaaaaa aggaactaac cataaacagt agaagtggca tttcaagcca ataaggaaat
86641 aatagattat gtaataccta ggattctgac aatttagaaa atctaggaaa agaacatcag
86701 agccctgaaa taaagtaaaa taaattccat aattattaga gatataaata tatgatgtaa
86761 aatgattaaa tactagaaga aaatattggt gttggaaaag aattgctaag gatttataaa
86821 atatatttat atagagacta tataaacaat ataaacatac aggctacata gggactattt
86881 aaacttctat ttattaaaat agagtatctg taaaagtaaa aatagaaatg aataaatggc
86941 aaaaaaNtgt aaaaaacaaa cataaatgaa aagttaatat tcttaatctc taaagaacac
87001 tctaaaatca ataagaggcc aggggtggtg gctcacacct gtaatcccag cacttcgaga
87061 ggcagaggca gtcagatcac ttgagtccag gagtttgaga ccagcctggg caacaaagtg
87121 agacacctgt ctcctaaaaa ataaataaaa ataaaatcaa caagaaaaaa caaatcagcc
87181 cacgaataaa taataaaata ataaaaagca aatatgtata attgccatta gacacacaaa
87241 acagatttta atattcttga agctaaatta attcttatta aaacaaaata ctatgtgatt
87301 catcaaataa agagaacaat taaaatgata aaatatagca acggaaataa tgtagaaata
87361 gagactgctg atgaagtata tataaacttt ctgcagggca atatgacaat aatagcaaaa
87421 ataaatcttt aaaatattgg gcaattcttt agtttgacaa ttctaattct agaaatatat
87481 agtaagaaaa caacccagcc ttgctataaa gactctacac aaagatattc atcacaatag
87541 tgcttataat gtgaaatctg gggagagctt caaatattta ataatagaaa attgcttaag
87601 tgtagtatat tcatgtaagg aaatgccatt aaaatgttat atagagaatt gtatttaatg
87661 gtaagaaaag tgttcatgat gcaatatact gtgaaatttt aaaagcagat tataaaatat
87721 taccttcagt ataatctcat tgttgttagt aaaactcctt ctctctctac atacacactc
87781 tcacacacag agacagaaaa attatctctc aatggcataa ttattgacag ttcgttttct
87841 cccccttgct tattattatt ttgtaataga tatgctatag ccactcaata aatatttgtt
87901 actaatttca aaaagtatgt attattatat ccaggttgtt catggaattt atcatgcaaa
87961 caactaccct cttgagagag tcaaaagtgg acactataaa ttatgaattc atataaacta
88021 tatcaggaca atgggcataa actgagtgtc ccaggcacgg caaaatgtgc ggataccctg
88081 gagatgtatc ttgactttaa gaagtttaca atctttcttg gggatagaag attaaaaaac
88141 atgaaaccat tagaaataat ctcccacaat tagttttgta aaagcaagtg cttaattgta
88201 cttgacaaac attagatgtt actggaggtc taagaagtaa gagctgaata ctcgtcagaa
88261 aagctccctg gaggaagtgg ggactgagtg atacttttag aataggtaag aaaaagaata
88321 atgagaactt ttcaaatgga ggttggggtt gtagaaggaa aagaatcaag tattcagagg
88381 tagctgataa cgatgttgta tatataaaat caccctgact tttgcaaggg gtccgtctat
88441 acagaagagt aaaaagtaaa caatctatta acaatgtact atcactgcct ccactttaca
88501 caggagaaaa tcgaggcttt agaaggttac ataacctgcc aagggtcaca tagctaaaag
88561 tggagagctg agatttgaac ctaaggagtt ggtttcagag tctatgcttt ccaccctatg
88621 ctgtactgcc cactacagtc aagctctggc ttcttgtata aatctgatgt cactctttgt
88681 gggactgctt ataacatttc ctcacccaga atccatacct cctcctttct gctctgtcag
88741 agctctttgt ctattcacac acagttcaaa gaataccttc tccatcaaat ttttctaaca
88801 attccaatct tggaattctc tcctaccttg gagttgagaa caccactctc ctaaatttct
88861 gcctacttct cagacactac ttctcaatct ctttgttgga ttcttctttc tctatatttt
88921 tSttttttta gtctactcat tgcctgtgtg agtaccttca cccccaaaac tacaatacct
88981 acctaccatt gtgctgaaaa ccccagctta cattgctctt ccacattcca taaatttcta
89041 tctggatgtg cacagacacc tcaaacttca tatttccaat actggcattt accctatctg
89101 cagtttttac tatcttatgg acagcagatg ccagaaatct aatcactgaa accagaaacc
89161 catgtacaat tcttgagtcc tccctctctc aagcctaaaa tcctggaagt caccaagttc
89221 tgtctatttt actctataaa taactattag attcttcttt catctgaact cccttagttc
89281 aagccctctt tgtttctcat atgattattg tagtaggctc tgaactggtt ttcctggtac
89341 ctgtctttcc cctgcctact ccaattctac ctccacatca acatttctca aatttgaggg
89401 gtcatagact tccaaggaca tttccacaat ccctgagaag tatacagttt ccatatgaga
89461 aatcactgta gctaaaagtt aaatactttt cttcagttga ttatgtatag ctaaggcaat
89521 aattcaagca ttagtaaata caaagccaaa atgcaggcac attacgcaga aaatgcctgt
89581 tcttgggttt ttaaaaaata cgttaaaaaa ataaggccag atgtggtgac tcccacctgt
89641 catcccagca ctttgggagg cYgaggtggg atgattgctt gagaccagga gttcgagacc
89701 agccaagaaa acatagggag accttgtctc tacaaaaatt tttaaaaatt agccaggcac
89761 agtggtgcgt gcttatagtc ccagctactt gggaggctga ggcaggagga tcgtttgagc
89821 ctgggaggtc aaggctgcaa tgagccatga ttgccactgc tctccagcct gggtgacaga
89881 gcaagaccct atctcaataa ataaataaga ccacaaagga aaaaaattct tacagagaat
89941 tattgaagta taaattgtgt caaaattgaa gaatataata aaccctaaga ttaatagtcc
90001 tgtccttgag aaagatcatc aagtgatgcc cccatgcttt aaaaaatatt ccaagctttt
90061 tagcataata tctaacattt cttagcagtt cctaataggt tacttctctg tagttacctc
90121 tctcactcct tccccatcac ctatagtgcc acaatctgta acctgtttga ggctctctca
90181 cacctttcat actgcaatat ttgctcgaac tattgacccc caaccttact catctttcct
90241 ttctaactaa ctaacttctc ctcatcctca aagccccagc tcaagaattt tcatttctgg
90301 aagaatctct gattttcttc tcttctctag tcttgaaatg gtatgtgtga acccacagct
90361 tgttgtaatg tcattgtcct ttctgtttaa caccctcagt tgctggtcct tgaagtctgg
90421 gactgtctta atctacttta aagtcttcaa gccaagtaca attcctaggc atgcaataca
90481 tgtttgttaa atagatggat gacctttatt tatatttatc tttccctttt ctgctacaat
90541 tagttaactc attcatttct tcattcacta ataaccactt atctgttatt ttctgggaat
90601 acaaaaattc ataaaacatc atccttgcct ccaaaaaaca ctttattatt gtgccatcct
90661 tctctctctt tctctttctc tccttctcta attatctagt tgtgtaggta ttttcttcaa
90721 aggaataaca ggtactcaga ggaacactta tacactgttg atgggagtgt aaattagttc
90781 aaccattgtg gaagacagtg tggcaattcc tcaaagacct aaaaacagaa ctagcattca
90841 acccagcaat ctcatttctg ggtatatgcc caaaggaata taaatcattc tattataaag
90901 acacatgcat gtgtatgttc attacagcag cattaacaat agcaaagaca tagaatcaac
90961 ctaaatgccc aacagtagta gacaggataa agcaaatgtg gtacataaac accatggaat
91021 actatgaagc cataaaaaag aatgagatag tgtcctttcc aggaacactg atggagctgg
91081 aggccaatat ccttagcaaa ctaacacagg aactgcatgt tctcacttgt aagtgggagc
91141 taaatgatga caactcatgg acacatagag ggaaacaaca gacactgtga cctatcagag
91201 ggtggagggt gggaggagtg agaggatcag gaaaatcaac taatgggtat taggtgtaat
91261 atgtgggtga tgaaataatc tgtactacaa accccatgac acaagtttac ctatgtaaca
91321 aatctgtaca tgtacccctg aacttaaaag ttaaaaagaa ataaaaaata aacagaatag
91381 tagggattca ttacaatatc ttctctcctc ctcataattc ttaaacctgt gtagaagttc
91441 aatacatatt tgtggaatag ccttattacg cctttaggga atggagcaat gccttgccta
91501 ttaatctata cttctatcag agtttcctgc tgtatggaaa acctgagtgt tacacttcaa
91561 acaaatgagg caatttatac atttttgttg ttgttgttgt tgttgttttt ggcataaatg
91621 tgctaagtga gctggagttt cagaagagaa actgaatgtt atatgtgaat ccaggtcagt
91681 agcttggctc tgactacaat actgatttca ttcaagataa ggaaactaat tggtatcaat
91741 tttagttgac attatatgtt agttttataa tattcaggaa tttttgaagg attatttgat
91801 ttttattttt atagatgttc ttgctcttta acactaaata atgaggaaag ataatagggc
91861 taacatttat taagcattta tgttggacag gcactgcttt aagtattttt atatgtatca
91921 actcattgaa ttttcacaac tcttagatct atttgatagc tggggaaact gaggcacaga
91981 gaacctcaag taacttgccc aaggtaagcc agctagttag catggaccag tgattcaaaa
92041 acaggcagtc tgtactcctt acaactgtat tatgctgcct ataaaaggac cactagacta
92101 ataattatga aaccagattt ttaatcttaa attagccata taatagtaga gtgcatttaa
92161 ggatgtcatc tgagtttcat tgtccttgta ccccttacag cctgtgagaa ggaaccttta
92221 tatagtcttt cataaactgt aaagtgttat aaattcaaag atttcttata atatgttaaa
92281 attactttta agagttgaga agtccaaata aaagcagaga gatgagagga aacactagca
92341 ctaaatgttc agacacccac tgaccaYgtc aagctgcaca ttttgttgct aaacctaagg
92401 gagagattag atatgaggag agtgggaagc aagaaggtat gaagtctggg tatttaggga
92461 ctaatagaaa tgattttctc gattagttta agaacattag caattactta cgggataagc
92521 acagttaaag gaatttctgg aaggaataaa actataaaaa ttagtcaaat tgttaaaata
92581 tcagaactgt gtaaaagcat ataaagtttc atcatatctt tcttttgaaa aacaaacaaa
92641 cacttcatgt tctagccagt tgctaatagt gatcagttga tgaatacacc tcatgtttca
92701 cgcatataaa attttacacc atgtctagtc cataggcatt agtgtaggag tcagaataca
92761 acctgaattc tgttcgcagc tttgtgcctt gtcgataaag cagtggacat ctctccacag
92821 ggagatatgt ttcctcccgt gatatagagg atgttgattc acttgtctcc tctctatttc
92881 gaaaagatgc tctgaagatt agtgagacaa tggctgtaaa gCtctttgca ctgtttacaa
92941 cataaatcat acacagacat actggcatga gaaacgctat catcatctct tttatacatg
93001 agaacaacca taaggaaaac aaacctcaga gacagtcctg tattataaaa atgttgggta
93061 gagatgccaa aagttgtagg gtaggaaaca gcaacaacaa caacaagacg gaatccaaag
93121 aatatttgca ttctataata tattctacag agtYtcagac gggaccttcg ttgagtgctc
93181 ccaagtatta tcagtagcca tgtttttttt ttctttttct tttttttttt tttttttttg
93241 gtaaaaggga taaatagtga tgtcaggaga tgctgcaaaa tatattccag tgataagcat
93301 atggcaagca attaatggat gaattcgcct ctcttattgc catgttgtga caaatactct
93361 aacttatttg gcctttggct ccctaaaggg tgctcagaaa tgctgtttag aaagtacttt
93421 tcatgaagag ctacaacaac aacaaaaaca aatacaattc aaatatctac taaacatatg
93481 aaggagtact cactctcatt agtaatcaga gaaatgcaat gatcccatta tagagccctg
93541 tcatttgaaa aaaaagaaag aaaatccaag tgttttttat tagaacgtgg agcaacagga
93601 attctcatta ccttctcaac cactttgtca aatgctttct gcttatctgc tgaagttaaa
93661 gatatgcatg acccatgaat ccactcctag gtatattccc ccacaaaaat gtacgcttat
93721 gtgcatcaag atacacgcac ataagtgttc gaaatatctt tattcataat atttccaaac
93781 tggatatgtt caactgcata aagaaagaag attggacagt aaattgtggt tcctttatac
93841 aacagaatgc tatatatcat gatatatatt atgatgtgca atcctcagct acatacaaca
93901 ttgatgaatt ttacaactat aatattgcac aaaaggagca aaacacagga gtatctacaa
93961 gatgagtcca tttacataaa atgcaaaaca aactaaattg aactgttgtt ttggcatgca
94021 tacatacgtg gtaaatcata aagaaaagta ggaaaagtaa aagtaaagtc aagatagtgg
94081 tgacctatct ggagaggatg aaacaggaaa ttatagggtc tttctgaggg gtgatcatag
94141 taggtattta ctttagaaca atttgttaaa cttaaaattt aaattttaca tactttctta
94201 aattttgtta tatttcacaa tttaaaaatt tataaaatga taattttaca aagcctaaaa
94261 gatgaattat ttgggggctt ttacttctct ttcatctgta caactgctga tgaaaatcac
94321 caggtgtgta taacatgaag catctcctta aatgacatgc atttattggg aaaaaacaca
94381 aagtccaaat ggtaaaatgt tagcaaggta aaacactgaa gatttttctc cccaaaactg
94441 ccaaacaagt ctggtttaac tgaaattgac agcaaagaca acagagcaca cttaattgaa
94501 ttttaatctc agtgatctct aagccatgtt tgaaacataa acagacatgt cagagttctt
94561 tatgatgttg gacatgaatt cattttcata attcaaaggc atactttgct tatgaatctc
94621 tttaacaagc tttggctgaa tattcttttc ttccagatgt ttgctgttgt atacacacag
94681 cttccatctc tttgcagatg atttaaaaca tagttagaaa gagtacattt atttaacaaa
94741 agagtaagag tacagaattc aaatagatcg tctgtgggtg tatataatga tgagtacata
94801 agtctcatgc agtcctcata aagacagcat ctattaatct gaagaagttt ctcttactaa
94861 tttgcaaacc taaaagcatt ttacattact aagatattaa taaataggtc aattaatact
94921 tttgatagag tagagagact cctaaataat ggacatgaaa aatgattttg taaaagtaYg
94981 gtttcatttt aaatccctgc ttaagcaatt tgtaggaggc aagatagtgt ggcagttgag
95041 aggatggtct ctgaaatcag atctgaattt gaataccacc tctaccattt actaacctaa
95101 acaagtcata ttacttctct gtgtctcaat agctacttcc tgaggtgttc ttacaaaaac
95161 acaaataaat aatccatgta aagaactttg cacagtgcaa atacatagtg agcacgcatt
95221 gagttttatt tatcatcatc atcatcttgc tgtacttttg taaactcacc ttctcccgtc
95281 tttttctact ctaaggtgct cttccaagct actttcctcc tcagctatcg ttcccctgga
95341 tttccagtaa gagagggtga aatcatgcct gcttatcttc tttctctttt actgatactt
95401 cactgaagtg aaagtaaagg aataaaaaca caacacaccc acccacaaag gcacaaataa
95461 taaaggaaac aagatcagca aaggagagat ttctactgat tttttgaaat cagaaaatag
95521 ctgaagtgcc ttagcataat cgaggaaatg gttacggaga gtaaattcca agagaaagtg
95581 gactaatgtg ttctcccgaa tccagaaaga cttactgctt ggaggcagca gaattaagga
95641 aggcaggagt ttagcgaggg gctgaaagtg tattaaaatt atatggagcc tttggatcca
95701 aactaactac ttcccttacc tactggaagc ggagtctttc ttttttggag gaaatcggct
95761 agagagattt gaaactgatg atactaggca ctgtagcaaa ctaggagagg ctgcaggttg
95821 cgaaataggt gcttatttga aagcctatag agtttgcaat aagaaaccca acttttttat
95881 ttgtcatgct ccccagacat tagcagtcag ttatatgccc caggcgcaaa tctcgcaggc
95941 agaaagtggg gtacccccta atcatgacct tggggaaaca tttcaaagaa cacaccggct
96001 ttgattgaac aacctatgat gcaacagtgc aaccctacag ttgacgagcc ctatccatga
96061 tcacagaact tctgacattg tttcttgcca tcattgtcat tgttgctttg ttttgtgtgt
96121 gtgtgcatat gcacctgtat atgtcttgct tttaactaag aatgaacagc ctcttaagga
96181 aactctccat aatgaagaag agaaactaga agaagcaaac atcaactgaa gaaagaaatc
96241 tacaggaaag agagagacag aggaaaacct caagaggata ataatctgtg accttagata
96301 aaacacactt ctgacataag aacatatacc attaaaaagg agaggatgag gctgggcatg
96361 ttggctcatg cctgtaatcc cagcactttg ggaggctgag gtgggcggat catgaggtca
96421 agagatcgag atcaccctgg ccaacatggt gaaaccccat ctctactaaa aatacaaaaa
96481 ttagctgggt gtggtggcat gctcctgtag tcccagttac tcgggaggct gaggcaggag
96541 aattgcttga acctgggagg cagaggttgc agtgagccaa gattatgcca ctgcactcca
96601 gcctggtgac agagcaagac tccgtctcaa aaaaaaaaaa aaaaaaaaaa aaaaaaagga
96661 gagaatgaga gtgctaaatt atttttaaat catgaccaaa ataaacattt taatagtaag
96721 gttggaaaat aaaaatgtgt gtgaacaaat cttctagaag accaaaaaac aaaaagacaa
96781 aaaaggaaga aaacaattaa gaaattaaag gatatgatct aggagatcca actaagataa
96841 tgagaattca aggaagaaag aacagagaaa atagagggca taaaattatc aatgaataag
96901 agcaaacatt tcagaactga aggactcata tctctggagt gaaaaggtct attgcgtgcc
96961 cagggcaatg gataaaaatg cagaccaaga caattgatca tgaaatgtgg gaacttcagt
97021 aaagaaaaga aggtcacaaa aatttctaca cagaaaaaga tcaggatttc accaatagaa
97081 aatactggag tgatgcctac aaaatgttca aaaaaataca tttttaacat agaatccaat
97141 tgcagttaat ccatgcctga gacaaaaaaa ggtttaaaca aagggctgcc tctgaatagt
97201 atatagaaaa gatttctgaa cagaaaatct taaaaactag cttttatccc caacttggat
97261 actaaatata gagacaggca agaattaggg gtcttcttgt cattcttctg agactctgga
97321 gctcctgaga cttctttttc actaacacca ttgaatccgg gaatcaacaa gctgcacaaa
97381 gttataaaga ttgccagact tcatgtttct ggaaaaaaaa aagtactatt ttttcataca
97441 gctgagacct ctgaaaagat ggctcaatgg gcaagatgcc atgagaaatc aaagccaaat
97501 actcattctg aagtcagttt tgctcagact ttgtatactg ccactcaatt tttcaagtca
97561 tcagaaacat aactaagaat gagtgacttt ttgctatYaa ctaatgaccc accattcaac
97621 gatcttgtat gacaaagtag aggagtatct tcttattttt aggcttatta aattacaatg
97681 gaaaatattc attaacaatc tcataatgag gcattctaga tgcttattct tacctaaagg
97741 gaatggaaca aaaaataacc aaaaactaaa aagatgaaga caaaagcctt tcttggtatt
97801 atagtattct tttacaaagg atatggttta tcatagtaat tgattccctc tgttacacta
97861 gagatactat tatattaaat gcctaaaggt taaacttagg taacagtgga gctatagttc
97921 tttcagaaat aaaataatta aatagctatg tatgccaaga aaccttactt tgaacatttc
97981 tattgtacca tctcattatt gctggctgcc tgattagcaa gcagtaaact tgggtaatag
98041 ctttcctctg atatcacttc aagtctactc gagttctaga attacccaga ctaccaggac
98101 tttacttgca caatatgctg aaatgtttat aacattatgc tttcccctcc taattctcaa
98161 gaccattttg ataactggct aagactggat aatatcaaca aatatgaaat aattataaga
98221 atcattcttt cttaaaataa gaagagtttc cagaaacaga aactaaaaca gaattcaggc
98281 taagctgcta aataggctat ctcagggatt ctcagctcat tcttgatgag gatagattgc
98341 tttttatttc ccttaagtgt tccgtattta tttcacaaat attacttaac tgtgtaccat
98401 gtacaaagcc gtgtgctagt taagctggtt actgtggaag aaataaaggg agtgggagca
98461 tctagctttt ttcttcaaag gagatggggg aatctgtggg agttagtggt aggggcacct
98521 acagaggaaa tWgctgaaaa ggaggagctg gggaagatga ggatgaggaa ctaagttgtg
98581 aataaaggaa gggcagagca atggtttttt taataatatt ggaatgggat ttgtttttag
98641 cactgctccc ctttccctta acagtcccct tgagctacaa ataaatgtaa ctggcaaagt
98701 ggataataag acttcagaat cttctagcta accatgatca atgtgtcatg

Following is a genomic nucleotide sequence of a GPX3 region (SEQ ID NO: 5).

>5:150384801-150484800
1     ggcttatgtc tacaatccca gcactttggg aggccgaagc aggcagatca cttgaggtca
61    ggagtttgag accagcctag ccaacatggt gaaaccctgt ctctacaaaa aaatacaaaa
121   ttagccaggc gtggtggcac acaagaggct taggtggaag aatcgcttga acccaggagg
181   cagaggtttc agtgagccaa gattacccca ctgcactcca gcctgggtga Yagagtgagg
241   ccctgtctca aaaaaagagg aagaaaggaa gggagggaag gagggaggga ggaaggagca
301   catacttgtc tgaggatcag taataatacR tataataata acatctgact ttcattgaaa
361   acatattatg tgccaggtag tattctgagt gcttaaatct attgattcat tctgtcctca
421   caactacaag gtagtcactg ttactattct tttttataga acagaaaatt gaggcccaaa
481   gagatcaaat aacttaccct gtccctaatt agtaatggag ctaggactca gaccatggta
541   agctggattc agagcctttg tacttaattg caatgcctct tScatatgYg ctaggtaggg
601   aacaaggtga tagcagaatt taatagtcag gattgatgca gtattcctga actagtatag
661   aatgagctag attaaaaact cagcaattag aattcagtag cgaatcaaat cctgagataa
721   gtaggatttg gcttatcctg tggccaattt tcctcgtatt aaacatataa attcatgggc
781   cttcacaccc ataaaagaat aaattagtat tataattttt aaaatatgat taaaattagt
841   gctttctgaa gtactggtta ggtgtttctg tttgcaaaga gaatttatgg ttttgtaaga
901   ttagtttaat gttgatggtg gaatagacaa gagccaaaag acactagaag caaagagaaa
961   aatgaaaatt aaactatagc aatccagcag ttagttaata aggctgcagc tcagatattc
1021  ctgttcctca tccagtcctt actttttttc tccaatcacY gttgtYaccc tggtcagttt
1081  taattctgct aaataagctc tccccactga ccctgttgca actgtccttc tttgttcctt
1141  tttactattc ctcaagatta tttagtgttt ctgcccagtt tgcaaaatcc tatttgtata
1201  tagccatata tactttgttc attgtcatat tctgtttctg tttctactac tgcaattttt
1261  aaaaaatgtg gttttctttc ttgaaatgct cagggggata gactccattt Rgccttgata
1321  tttcttgtag gccatatgta tattatgcat gaaagtaaag attcaatctc ataatatgac
1381  aaaaagttat gagtcataat acattagcag atatatttac cagtatatag ttaattgtgc
1441  ttctttctag aagtctggca agacttccaa ccacatgcag aggcaccagg agaaccaata
1501  aacacttttg aggcatgttt cattaataga ccagggaact gagacagagg aaaggKgtga
1561  caactgtaat gctcttgaaa atatatttca tctgagccca gactttattt caaaaattcc
1621  aaaaatgtgg ctgttatggt aggaatttga actaYttgga tttatttagt ggcaatagaa
1681  gatatgccac aMaaaaaaag agcatagtga atgtgaaaaa gcctccttcc agaagtcaga
1741  cctcattatg caaaagagaa ccataagaga gaaaagccct taatatgtac taagtgtggg
1801  aRaaccttca gcagggaatc aaatctcatt atacacctga gaatccatac tcaagagaaa
1861  acctacgagt gtgttgaatg tagaaaagct ttctcccaca agtcacatct gttgaacgtt
1921  agagaattca caccagggag aaaccctatg agtgtggcga gtgtgggaaa gcctttttcc
1981  aaaacttaca cctcagcatt tacaggagaa ctcatactag aggaaaaccc tatgtgtgtg
2041  atgaatatgg gagagccttc Wgcctgaagt cacacctctt tgtacatcag agaatacaca
2101  caggagagaa aWcttatgtg tgaactaagt gtgagagagc tttcagtgaa gtRtcatgcc
2161  ttattagaca tcagaaaatt tacactagag agaaacctta tacatgtaat gagtgttaaa
2221  aagttttttc tcaaaagcct gacctcatac attacagaat tcacacaggg aaaaaaactc
2281  atgtaaatgc agtcaatgtg taaaaaaaag ccttcagaca tagatcagcc ctctgtcgac
2341  ataagaggac tcataaagaa aaacatccct gaacaagaaa taaatatggg aatatcttta
2401  actagaactt gtagtgatag aaagccttca gtcaaaagca aaccttatta tttatcaaga
2461  aatcttactg aggataagcc tgtttaatat atttataaaa gcgcgcagtt atgtttctat
2521  agaaaacata ttccagatgt gattcctaaa tatttcttga ggatattaca gaaaatacat
2581  ttttattaga gaataaatgt tcactgtgga ccacaaaggc ttttaaaata ttaataagtg
2641  aactagtcag aacaaactta gaaaacRata tgtggacaac ccacaagata tgaatgttac
2701  tatatgtgcc actacttatt gctgtaagtc ctggctgcac tacattttgc tatgcatcat
2761  ataaaataag ttatatgtgt tggaattgca tatgtgaatc taaaaatttg taaatataaa
2821  ataattacta tatatagaat gccatttact aagactttct acattaaata ttaccatttt
2881  tcccctaaag aaaatacaag ttataaccaa aaatacagtg ttcagtaaga gttctaaagt
2941  gtaaaataaa cttttttctg ttgctttctg gtatgtatga cttctgctgc ttcttaatga
3001  gaagtacata aaggtgttaa ttacataaaa tatataaata taaactagat atcatataag
3061  tctcagtagc aaaagaaaat agtcatggcc tcttacaaga ctcactgcaa tttaRaaagg
3121  aattcttggt gggtacaatt acccttttta catactgtta tggactgaat tgtaccttcc
3181  caccaRattc atatgttgaa gccctaatca ccaatacctc agaatatgac tgtatttgga
3241  catactgcct ctaaagagta attaaaataa aaccattagg atgtgtccta atctaatctg
3301  actggtgttc ttacaagacc tagagagtag gacacacgag gagacatcag gggcaggtgt
3361  acacagagaa atgaccatgt gaagtggcag caagagggct gctatctgct agccaagaag
3421  agaggcctcc aaggaaacca acccttttgc acattgatct cagacttcca gcctccagaa
3481  ttgtgagaaa ataaatttct gtcatttaag ccactcactc taatattttg taaggcagcc
3541  ctaggaaaca aatacaaaca ctactgctct ttttccctgg agtactttgt aagcttgagt
3601  atacaaagta taagtactac gtaagccaat aatatgatag agtgcttgct atactgcacg
3661  gcatctctat ttggcatttt ctaattcacc ttgtgacaga ttagtaagag agatattttc
3721  ataaggattt tacaattcac aaatattata aagacttttt gcagagagaa ttgttcattt
3781  ttaaaattcc atctaacagt aggggtaggt attctgtaaa tttccatgtg agtatccatg
3841  ccattaaaaa tcaacttgtt ccttatttat aaattaaaag tggggcctca ttcttctaaa
3901  gcaaattttg acagggagtt aagattacaa ggcagaaacc tacaagaggt gttaagagct
3961  cttattctgg atttagacag atgtagattc tgaatctgct ctgagcctca atagcaccta
4021  gtttgttgga tcattgcaag gattatacaa tatcatgtat gtatggtgct cataagatgt
4081  tagctatttt atattattta aggatataat tcocattcta gttatgaaga cagacaagga
4141  aacaactcta attaggatta tattagagag aactgtaggg agtcatgtgt atcattctgt
4201  ttcattctca caaccatgtc agtctttcag actcaggtgt gggcctggaa ccactgccct
4261  gaattgcccc tctacatcta ttctataatt gcttgttcag aagcaatacc attgctgata
4321  ccccaaactc cttatcttta tctcatctac tgatgagacc cattgcttgg agggtagtta
4381  catggaaagc cattcacaaa acaccttatt aactttgtgg gtgaaaaaaa agtgtatctt
4441  aattgtatta agccactgag atttagggat tgattattaa tagcaaatag caatcatttt
4501  ctagtagaaa gaaattggtc ctggaagtgt cctgctaatg taataaaaac ctaaaatatg
4561  gcataggcct agtagttgag cagtgagaaa actgatgtca gaagtggaaa gatgtttatc
4621  tatgttttgt actagccaaa catttggttt gggccaggcg tggtggctca cacctgtaat
4681  ttcagcactt tgggaggtca aggtaggcaa attgcttgag cccaggagtt caagaccagc
4741  ctagacaatg tgatgaaacc ctgtctctat aaaaaataca aaaattagcc agatgtggtc
4801  gtgtatacct atagtcccag ttactcaaga gcctgaggca ggaggatttc ttgagcccag
4861  gaggttgagg ctacagtgag ctgtgatttt gtcgctgtac tccagcctgg gtgacagagc
4921  aagaccctgt ctcaaacaaa acaaaacaaa acaaaacaaa acatttggtt tgattgtctc
4981  ctctacacat gcttactttg ttgacagctc cagaagataa ttagaaaaca gagtattagt
5041  aatatttgct gttttactgg ctgtatttgg aagctattgt aagaaaaaga taaactcaga
5101  gaaaattagt ggattataag caaaaatgaa agaaaataga gtcctgaaat ttgagacctc
5161  atggagttta aaaaatttga tttctagact tcaaatgtta ggagaataat ttaaatggtt
5221  ttgagtgtat caaagaccta ataaacattt gctagtgaaa taaagtgact cagaagaaag
5281  attatattaa gaaactgaaa ccttctcgtt caagcttgat aaccttacag tagtctgtta
5341  ttcacctacc ctagctccat gatggagaag ctcaatttta ggcagctaca gccaagaaga
5401  ctggggctcc catacccaac ctagctccca cttgtagagc agaggctctt gccaaggtaa
5461  gagaggctaa gagtattggg acctcaattg ccctcctcct cagcttttct ctatagagta
5521  gatgtttcat gccaggaaag gcaagccaaa aaaaagatca ttctcccagc tcccactaat
5581  aggatagagg ctccagactg gcaggagtac attaaaaaga Yctcaggcct tcatcttcct
5641  accaagatct cacttgcagg aaggagtatc cctgctaaga agcttccccg gattccactg
5701  gtagggcaga ggtgtcactt caggaaaatc cggcKttccc ctttgtcccc cgcttctgtg
5761  caagagtgta gatgttatat ccagggaaag aagcaagatg ggagggccag aagctcctca
5821  gctcagctSg ggggctgact gtatttaatc ataacatgaa gggttgcatg aattaaggca
5881  ttgttgaaaa tagagatctt agtggcaagc acttaagaga attctaatag tttcataaaa
5941  caagtagcaa caaatgaaac agcaagtcac ccggtttaac agagtgaaca agagaaagag
6001  ctaaaactgg aagtctgtgc atatgtctat gtttcacaca ttcaggacca accataacag
6061  gtactagctg aacttgagtg gaattgaaag aatttcccaa gccacacgct gatacattag
6121  caaagagtgg aagtcttact agctcaagga gtgtaagcac aacccctgac caaacactgg
6181  ctaaatgtta agatattttg acccaacggt gacttctagg aagccaggct tggggtaaaa
6241  gctacacact gtggggtggg gggcggcggg gggaaataga gtttacaaag ttggtgcaag
6301  caagccacta aacaaactaa caaaacagag ccacatcaat aatccctaaa ggggatgtgt
6361  cagaatccag agttgctaca atatattatt tcaaaaacta ttagagatgc aaaagaacaa
6421  aatagtataa cccttgtaaa ggaaaaacag catcaataaa aactgctttt gaggaggtct
6481  agatgttgga cctgtaaggc aaagatgcca aagaagctat tacaaatatg ttcaaaaaac
6541  taaagaaaac catgttcaaa gaatgaaagg aatgtatggt gacaatgact catcaaatgg
6601  agaatgttag tagaataaaa agtgtttaaa gactcaaata aaaattctgg agttgaaMag
6661  tagaatacct gaaatgaaga ttcactttag gtactcaaaa gtagatttga gctggcagaa
6721  gaatcagtaa gcttggggct cgatcattcg atattataca gtcttagaac agaaagaaaa
6781  aataatgaag aaaacctgag cagacttctg gtttctagtc tgacatgtaa agagcttgga
6841  agttgtcaat cctatcatca caaccagaaa aaagtctgaa aaactggaaa ttaacaattc
6901  ttagatcctt caaagaatca aggtcacagg acaaaccact gccccagaaa ctagagagac
6961  aggtagatgc tgagaatcac aggttcactg acagcggaag tccaccacta aagtcaatat
7021  attttaggaa cacttaaact acaattgatg aattgctaga gactagcaat aaaagtgcca
7081  gcattctagt tgatgaaaat gttgtgacca gaggcttaca tgaacctaat cctgtattcc
7141  tcttggagca attattcagt atttactaat acagtgtttg tggtggattt atttaacata
7201  agtactgtga ataataaaat caactgtata tgaaatcttg actatccatt tacatgtagg
7261  agaatcaaca aatggtattc tccacaaatg gaatcaaccc ctagaagttc ctgagaaatc
7321  cctgttgagt gggaaaggac ctagagccaa ggagacaagg ttggaggtga ctggtcctct
7381  tcctttgttc ttccactgaa attacttgag aagcatcatg acacatcctt atacacaggg
7441  tatggtggaa aaataattgg cagtcaaaaa gacttaagtg tatgaagact taagcatgat
7501  acacatggag aaggtgatgg tgtaaagaat gacaatggcg tctgagtaac attctgaagg
7561  aggagggaag ttcaaaaggt gggatggtgt gcctggatga gacacatgag ggagaaggat
7621  tttctatggt gcttctgtta gtttagcagg tagaatgccc ctgtgaaggg tacaggaagc
7681  atgtggcaga gtgggtgcct aagtctgggt ttagggagac tttagaaaca atttgaaacc
7741  cagggtctta ccctctgttc acctcataga acagtaaaat cccttggatc aggcaaactc
7801  tgatgaaaaa cccagactgc catctctctc ccagatggcc aattttcctt ctggtgctgg
7861  cgaatgatga agttgtcttc aggaaggcag aattctgctg Sgtgctctcc tcttctgagg
7921  agttatacca ccacacagcc tttgctccca gaaagagtca ccatcttgtg ttctaacatg
7981  gatcccttgt aagcccatac aaaccactcc atccatactt aactgctttc tccacaaagg
8041  caactgagtc taggagccaa tggactatct cactttattt tttaaaattt acccagggca
8101  ctttttcatg ttctttcttc tccctggaac ttactagcat tatggaacac aaggatgtgt
8161  ttgtggtcat cttctcttta caagataggt cacatagacc cataagtagg ctgtgtatac
8221  ttttcttaca aaggaggtgt cacttacctt gggaatacca gccagccaca tatgaaatcc
8281  cagacacagg agacccctaa gaaaaaggcg aggctgtctg taactgcctt agcaactaag
8341  ccaccagtgg attcaactct taaaattaca gcaccatgga aacacaaaat cacagaattt
8401  tggatttggg taacactaga gagctcacat aattaaatac cttaatagaa aattctcatt
8461  tactaattag aagtttttaa ccatgtagtg taaccatgta tacactattc tcaaagagaa
8521  tgggaatatc ttcttcagtt acttaatgaa aacatacaag gaatctgaaa ccctaacata
8581  ccagaatcca tgggacaaaa ggaaaattat ttgatcatgg gattcagaag catttactgg
8641  atcaaggttt agagatgctg aaagtagaga atcagtgtta ggttttggag ggaaggcaag
8701  ggttaaagga agacagagag agaaacagag caactcaaca gcaaacacag gtatattgca
8761  gacacctgtg gaagcgggag acccgcttaa tgccaaaacc caccaccact tacaagcagc
8821  agtacttaca ggtatggctg ggagaagtct ggatagtgtg gcttgctgcc cggcaggata
8881  tcaataagat gtacccatga tagggtggtt tgaccctttt tctggtggga tgtcatcatg
8941  gtgttccttg ggcctttgcc cagtaagaca ggatagggag gtttctttag ttgggccttt
9001  gcctggtagg gtataataag aatgtttttt ttattgggcc tttgtcttcc ttgtggtcag
9061  gtggccaggt aggatgtttc tcatggccca aacccccgtg gaatgtttca ctttgaccaa
9121  ggtctgcaaa atagcagggg acttacaaaa tggtgccgtt tgcactaaca caaaggtgaa
9181  tggcatgggg aatggtaggt caagaaagct gcttccttaa tttatccctt ctcttgaaat
9241  gtcttcatta ttaagacttt cattttaact gcctttggag ttaaaatata aaatatgaat
9301  tttaacacag aagtgtgaat gctggtattt ttgaaatcaa gttcttataa tcccaggcta
9361  gtgacagctt gcctgggcaa ctaagtaact taagttcata agtaaacact aactctgctg
9421  aaactaaata actcatttta gcttctgaac attttaagca tttctcattc tggataagaa
9481  agagaaatgc tgaagcagaa tttatttcat ctgctttaga tgaatttagg aatcccttgc
9541  aaggcataaa actaattttg tgatcctttg agtaatatgg atttctgagg aaattctaga
9601  ttcagagaag tgctgggcca gattttggtt aaggcaatga catggaaaaa tttttcaaag
9661  aagaggtaaa aatatgtagt ggagtttttt tgtttLtttt ttttcataat caggagtttg
9721  agagggtata atggaagttg ttaggttttg acgggaaggc gagggttaaa gaaagacagg
9781  gagagagagt tagcagctct acagcaaaca caagttttat gtctagcaca agacctgcgg
9841  aggttgggga ccagcttaat gccaaagccc actgccgctt acaggctggg gtacttatag
9901  gtgtgggtgg gaggggtctg ggcagtatgg cttgctgcct ggcaggatgt tgataagatg
9961  ttcccatgat gagatggttt ggtctttgtc ctggcaaaat gtgatagtga tgttccttgg
10021 gctttttccc agcagaatat ggtaagaaat tcaggtggtt gagcaaaatg tttctcatgg
10081 cctgaatccc catggaatgt ttcactttga ccaatgtctg cgaaatgatg gggggcttac
10141 aaagtggtac agtttggatt aacattcttg ccttctactt cagtctaaaa ggaagagggg
10201 cattgttgat tatctagctg cttcctgctg aataggggca ctgtaatcag ggcttgggtt
10261 ttgaagcagt gggtgtccaa cttcagagtt gttttcctgg agatgttgat accagacttg
10321 gtggggaaag atggtatcaa tgtgtttctg ggtggctgcc tagacaaggt agttctgcct
10381 ttgggagata aagtgggata tgaaggtaag tatacatggg ccaattgtga gtattaggag
10441 gaagattagg ggccctaaga aaggggtaac cagatatccc tgtgccaagt gtagctgaga
10501 tgcttagtcc tgctaacagg ggaatgaagt gtatagccca cttggtgtgt gtggaggagg
10561 aggaaatagg gattactaaa gaacctgaat ggtttgattg ttaggcaaaa tgttaatgtt
10621 tggagattga aacaccaggg tacatgttcc tgaccaactt gccagtaggc aaagaagaga
10681 gtttgttcca cacagaaaga aaagactggg ggttgacagg caaagttgta agtgaaggca
10741 gtgagccatg agaagccggg ggttaaatcc tgaacaaact tttgtcccaa cttttttgtt
10801 tttccaggtt gaataactgt taacaaggat agtccctgtt agggcctgat agggatattg
10861 gtggtgaagg ctgtctggtt ttagacagag agagagaaat gggtttttgt taacagccat
10921 tgcggtcctg acagggaaga agaataaagg ttgcagttga gggaattgtc tgtgcatttt
10981 tttagggagt tcccttaagt tggatgcatg gagaacagtg ctggcagaga agggaggggt
11041 tgggaaggga tgttttatgg aattcagttt gtaacatgtt tagtttacag gccatgagac
11101 gaaagagggt gccagccctg cgtgcgatgg tgcaggtagg tttgatggat tgcagggaaa
11161 ggtgtttagc caagcaagat tataaacagg ttttgggaag ggactggttg agtaggtgag
11221 atgcaggttt actttggatc aagtttctgt ggttaggtta acaggaaggg ctgtgaactg
11281 ctggatttgt gtggacaaac aaatttaaca attggaagaa agaggagagt gtgactgatt
11341 taagaggtaa tgtgtgaggt tgacaaagga agcccaactg ccagaggtta tgggatttag
11401 agtatcccac agcaaagaca aaaggggaaa aagaagattt gagtacRagg gaaattttgg
11461 aaggtRccct gcagccatag ctcctgggtt aatgtgagga attggagtgg actatcoagg
11521 gatgtgggga gggaatatca ggaaagatct gaaacaaggt gtgagataac agatttgaac
11581 ttggaggcag agagtgtaat ggaccagggc cttctggact ggcaacttct ggaattcttg
11641 ttaagcatag tgaggttggt cctatgaagg aggaagaata ttttgggggt gagaaaactt
11701 gtggatgtaa gtctggtgct gtttttagtt tggaatggtg gatttagtgt ggaaaggatg
11761 ttagttttgt tgccacagga gtagtcagga ttacctggtg aggacccgtc cacttaggtt
11821 ggagagggga gaaggaattg gtgatttaga cccagttccc tggttgtagg ggcagggagg
11881 agtgtttgga ggatggactt ttaggctggg gcaagtaagc atttgcgtac cgtcttatta
11941 gatgttgagt gaggtataat gccagccaag tatcctgtat caaRgaggtg gaggggcggg
12001 ctgggggcag atacagggag attttygagg ataaaagggc attggtacat gaattcaaat
12061 gggcttaggc tgaggggcgt gtgagcaatg gcttgtaaat tcatgagtgc cattggaaga
12121 agtgaagttc aggccatttt aacctctagg aagtttggtt atttgttgtt ttaaaaaggc
12181 attgacctat ttacttttat ctgaagattg ggggcagtaa ggaacatgaa aggcccattt
12241 aatgtttaca gcctccacca actgttggtt aacctgtgaa acaaatgttg gcccactgtc
12301 taaccggatg gaggagggga gtccaaacca ggggatcata tgggtgagga gaatagaagt
12361 gatggtgtgt gccttttcgg tggtggtagg aaaatctttt atccatccag agaatgtatc
12421 tacaattgtc agaaggtacc aggcccattt tatggggacc atgagggtaa agttgatctg
12481 cccgccctgc cctggcaggt gtccttgggc ctggtgtgtg gggaaaggag gtggtttgat
12541 agctccctga gaggaagttg tttgagtgca aatggaatat gccttagtaa tacctatgag
12601 attggcagcc atggcgggaa aatatatata agcttttaag agctagagta gggggcagta
12661 accggcatgg aaatggttgt gcacatatga aagtacagaa agttcctggg acttgggcaa
12721 gacaatttta ttattggggt agaaccatcc tcctccccaa atagcaccag cccgggtgag
12781 tgaggtttgt tcctcctggg tatatacagg gtgtatgctg ggaaagatgg gcaataatga
12841 tgaggtttta agggctgcct gctgggctgc agagtttgct gagaagtttt ccttggttat
12901 ggcatctgta gccttttgat gtcccttaca atggataaag gtggccttta gtggtagttt
12961 agctgcctcc agcagtttgt gtatgagttt gccatttact atgggggttc cttttgtagt
13021 taggaaacct ctttcctgcc agattaaggc atgagaatgt aggatgtggt atgcatattt
13081 ggaatcggtg taaatgttaa ccctcttgcc tttactaggg tgaaggccct ggttaagaca
13141 actagcgcca cctgttgaga ggtagtatgg agcgggacag tgctggattc taggagttta
13201 ttttcagcaa tgatggcata gccagctgct ggacatggct ccctaaaaca gctaaaaaga
13261 tcttccatta atgaatcatg tgggtgttcc ctgcaaaggg gcttttgaaa tgtgttggaa
13321 gggggagaag agggagttta agaggtccag gcaggagtga gagagcttag agtcagaggt
13381 gtttacaggg aagagggtgg ctaggttgag agctttacat ttctagaagg tgattagagg
13441 gttacctatg aataaggcat gtatctatga ataaggcatc ctgtaagcag gatggtggga
13501 gggatagaag agatcaatgg ctcatgagat cctgtaggtt atgggaagat gcagtagtaa
13561 tgtgttggta gagagtaagt ttctgtgcct ctgaggcaag taatgtggct gcacccaaga
13621 tttttgggca ggatggccag ccttggatga tagtccattt gttttaagag gtatgcaatg
13681 gcttgtggag cattgccata tgtttggcag agtagtccaa gagcaaggcc ttggtcagaa
13741 tgtacacaca gtgaatgggg ccagcccctc cacacctgtg ggtatttctc atcaggcagg
13801 aegagagact gagaaaagaa ataagacaca gagacaaaat atagagaaag aaaagtgggc
13861 ccaggggact ggggctcagc atacagagga cctgcaccgg cccggtctct gaattccctc
13921 agtatttatt aattactatt ttcactatct cagcaagagg aatgcggcag gagaagaagg
13981 tgatagtggg gagaaggtca gcaagaaaac atgtgagcaa aggaatctgt gtcgcaaata
14041 agttcaaggg aaggtactat gcctggatgt gcacgtaggc cagatttatg cttttctcca
14101 cccaaacatc tcagtggagc aaagagtaac agcagcattg ctgccaacat gtctcgcctc
14161 ccgccacagg gcagcttttc tcctatctca gaattgaaca aatgtacaat cgggttttat
14221 accgagacat tcagttccca ggggcaggca ggagacagtg gccttcctct atctcaactg
14281 caagaaggct tcctctttta ctaatcctcc tcagcacaga cccttcacgg gtgtcagcct
14341 ggggggcggt caggtctttc ccatcccacg aggccatatc tcaggatgtc tcagtgggga
14401 gaaaccttga acaatacctg gctttccagg gcagaggtcc ctgcggcttt ccgcagtgca
14461 ttgtgcccct ggtttatcaa gactggagaa tggcaatgac ttttaccaag caacctgcct
14521 gtaaacattt tgttaacaag gcacatcttg cacagcccta gatcccttaa accttgattc
14581 catacaacac atgtttttgt gagctcaagg ttggggcaaa gttacagata aacagcattt
14641 cagggcaaag caattgttca gggtacaggt caaaatggag tttcttatgt cttccctttc
14701 tacacagaca cagtaacagt ctgatctctc tttcttttcc ctacaataca gagtagaagg
14761 cttggtggag ttgggcagtc ccagtactgg ggccattaaa aagggcattt ttcagctttt
14821 taaagtggga gttgatgggg caagctgggt tgaggggttt taggatgagc ccatgtgagg
14881 ccatatagaa cagcttggcc agcaagccaa agttgggaat ccacagatgg aagcatccca
14941 tgcagcctga gaaggagagg aggttatttt tcgtgtgggg aagaggcatg tcccaaatta
15001 gctcctttca ttgggttgga atggcctgag aattaggggt taggacaagc ccaaggtaag
15061 tgacctgcat ttgggctaac ctgagccttt gtgggtaaga ccctaaaatt tacttggtat
15121 gtcccacccc aagatagggg tagggcactg agggataaac aggaaagagt gggtgaaggg
15181 ggtgatgaat aggttgcata atagaggacc agtctgtttg tgcctagagg ggattctatc
15241 gactcccaca atagagatag aagaactgag gaggggctca gaatattctg gtaaaattga
15301 ataactagcc tcatatttaa tgggaaagac atgggcttag cagacactga cagcattact
15361 cttggttcca aggtggtgat ggtggtggga gtggeggatt ctgggcccta tcagtcttca
15421 ggtagaggtg gtgaagtggg ataaagcgtt ttgctgagca cagtctgaga tgtgctccct
15481 gtcctccagg gtgagggagg aagagaggat gacgtaagtg tcatgccagg taatttataa
15541 gattgagtga catacagaaa ttccttgtgg aaggaggtgg gatccatgga aaagaagcca
15601 agtctttttt caagctggaa gaggtcagct agggagaagg aaacataaac tcagattata
15661 ccttcggccc gcgtgacttc ccgcaagggg atgactttgg aaggcctttg ggtgtatgcc
15721 tggttttcag aggaaggggg ttaagtgtga gactgggtat gcggtggaga agaaaggggg
15781 aacagagggc aacaataagg gaKtgaagga gccggagctg aaggaggagg aggggatggt
15841 ggtgggagaa ggactgaagg aggggttttg ttgtccgggt tgaaattaga ggaggaaggc
15901 ttgtctgaag gaaggggcat ttttgggttt ggaggttcca gattagacat ctgagagtgg
15961 tgtctggagg aagttttgag gagccttgcc ccatactggt ggctggaagg gtgagaaata
16021 tagaggaggg gtgtgagtac cctggagggg agtaagtacc atgattcctc ttgggaggag
16081 ggacataaga aagccagggc atccctgcag ctcttcacag tcctctggag atcagagtga
16141 ctggagtggc gagcattctg agggcgtccc ctgagaaggc agaccatggt cacctggtga
16201 ccagggagac ctctcaccca gcactgggat tttctggtga aggagcagaa gcatgaKagg
16261 aatctggaga ggaggtaaag ggagactcac ccattaattg gagacggcgt tggacgtgat
16321 gttctgcaat gggggtgatc cttgccggag ccacccagaa agggggagaa agaaaaaaga
16381 ggctagagag tttgatcagg atctagaggt cagcccaggg ccaaaggaga gagaataaga
16441 gaaaaagggc tgcaaagagg gagtctactc aggatctgga agcaggcctc agtctaattt
16501 ttgctgcttg ctgctttcca ggttgcaaga gaagacttac ccgtttagaa cccaatctat
16561 gtcctgggtt tctgcgccaa aatgtagtgg agtttttttc ataatcagga gtttgagagg
16621 gtacaatgga agttgttagg ttttgaaggg aaggtgaggg ttaaagaaag agagttggat
16681 gctttgcagt aaatgcaggt tttatgtcta gcccaagacc tgcggaggtg ggggaccagg
16741 ttaatgcctg aacccactgc cacttacagg ctggggtact tacaggtatg ggagggaggg
16801 gtctgggcag tatggcttgc tgcccaacag gctattgata agatgttccc atgatgaggt
16861 gacttggtcc ttgttccagc agaatgtgat agggatgttc cttggacttt ttcccagtgg
16921 aatgtgatca gaaagtcagg tggtcaggcc gatgtttctc acacagagaa ccacttccac
16981 aagattaatt tccatactcc ttccaccact tgtttgaacc ttcagcttta tcctattaaa
17041 ttcaaaacaa tcttttaact ctaggcaaca atttatattt ccatggcttc ttataacctt
17101 ttagtaaaac acacacacac agacattttt ccaaacacac cttgcatgta aaaatatttt
17161 tagtagtatc aattacatgt tataatgatg actcttagca atcttacttt taatgtaaaa
17221 gctggtaagt tatgttaatt atgtactagg tgctgataag gtcttactat tccagcatag
17281 ctaggggtgt ggccaactcc acatgtcccc aggccttacc tagctgtaaa gcaggcaagt
17341 caaacaattt tcaaaagcca aagaagcagt ttatgacctt aaggcactta gcaaacctaa
17401 tatttgaaca taatttagat gacatgtttg tattttgaag acatttgtga aaactttaca
17461 gaggagataa acagtgactt tcacgtctca tttagccagt ttgcacagag agaaagaggt
17521 cagagtctga ctggtaggaa attcttatcc ttttgccagt aggccaggtt tctgggtttt
17581 ctctccctga gcagccctag tgaMcctgct caactgtatg caaacaaaca cactaccatg
17641 aattaagaac atttacaaat agtttacaaa ttttggagaa attaggcaga gagagaaata
17701 tgacttaaat tctgtttaca aaagtatact caacatactt aaaatatcaa taagcctaaa
17761 ctccaaaaag ttagtttaag gttaaaaagc tgatgtgttc cattaactcc tgtgggtctg
17821 acaaaggtag cctaggaatt ccagataaat ggaacaaatg atgacttgct agaaatgcac
17881 aggaaacaaa ataactatcc acaaaaccaa ataaaagcct tctagaagca aaaataaata
17941 aacaggaaag caaccctaat tttttcttac tcaatttacc ctggaggcta aagtgttgcc
18001 cagggcccca aaagagccac ataataaata ttttattcct gatacacaat tcaatatcct
18061 taagttcacc aatatcatca tacatcctgt gcaatcaaga agttcacttt aggcccatga
18121 ccaataagca ctccagtgcc agcactatcc atgcaaaaca gtaaacatag tgtgaagcag
18181 tgcaagcata tctgggaaat ttggctccac actaaatcca gcttcaagct taactatata
18241 aaaaaaagaa ttgccaaact gccaatgcat ttctttacaa tagttattat tttactttaa
18301 tcaagactaa gagctttaac tacgaaaatg ttaactagcc caatgtctcc aattctttat
18361 catgttttaa agaatatttt attatctaaa ctttttccac atccttctcc cctaatcact
18421 ggttctttac tacattgttt cacaaataat tttttcaaat gtgtaatttg aactaactbt
18481 tagataactt cttaattaga caaaattatt ctttttctca gtaataacac aaactttttg
18541 gcacattttg tatacagaac tctgtgttaa ctagaagtat tatccttagt aacctaaaat
18601 tttagtgaaa ccctaaaaag aaatactgaa ctatcagata tgggcattta tagataagaa
18661 caattccaca atttttagaa acatatttcc ccatatcaca acccttcctt aattggaaat
18721 gacccaaata ttcaatgagc atcaaaatta attttaagct tttaatttac acaaaaagtt
18781 tacctaaaac atttatccca tttactgtac tcaattcttt catttttaac agtttatcta
18841 gattacttct gtaaactgag atattagaca ctatcactta aagttagtta tttccttgct
18901 aaccatgttt ctaatagcca gtgaatatca agtgctcacc taaacctgag agtctcagaa
18961 ttaaatatat aagtattttg ccaataactc agaagacgta gctaacatta aattgctctt
19021 atttgtcaaa aaaaaaaggc acattttgtt ttggctgggt ttgtagtttt ataacattct
19081 atgccaaaca ttgacatctc aaaatattta gcaaacacaa acataaaatc cagacaaaat
19141 gtatgctgac aattctgaag gcatttctat ttttattcta ccaataattt taaagccagc
19201 ttgtttagta aagttatact taagtcactt taacttaaaa atcgcttagg cttattaatt
19261 tatgagcgct cttttactta taagccaatt tggcaaacat aacatataag tgtacataca
19321 aataaacaca tctagacatg tatacacaca cacaaatgaa gatctaatag cttgaaaccc
19381 tagccatgag atagcaacac aagcttaccg gttttacttt gtttgcccca gtaggtaatg
19441 caatgaaggc tgtgaaccaa aattttgggt aaagcaattt ccatggcagt ttgatttttt
19501 ttgtttcttt ttcttttttt tttttttctt tgagatggag tcttgctctg tagcccaggc
19561 tagagtgcag tggtgcaatc tcggctcact gaaacctctg cttcccaggt cctggttcaa
19621 gcaattctcc tgcctcagcc tcctaagtag ctgggattac aggcacgtgc caccatgtcc
19681 agctaatttt tgtattttta gtagagatgg ggtttcacct tgttggcctt gttcatgtct
19741 tgaactcctg acctcgtgat cYgcccgcct cggcctccca aagtgcaggg attacaggcg
19801 tgagccaccg tgcccagatg gcagtttgat tttttttgtt tttgtttttg agacagagtc
19861 tcactctgtt gcccaggctg gagtgcagtg gcgtgatctc agctcccact gcagtctccg
19921 cctcccgggt ctaagtgatt ctcctgactc agcctcctga gtagctggga ctataggcac
19981 gcaccaccac gcctggctaa ttttttgtat ttttagtaga gatggagttt agtagagatg
20041 gggtttcacc acgttggcca ggatggtctt gatttcctga cctagtgatc cacctgcctc
20101 ggcctcccaa agtgctggga ttacaggcat gagccactgc acctggccct agcagtttga
20161 tttttaaagg tgaaacctcc ccagactcca aagaacacta agaccaaaca gtaccaaagg
20221 agaacatcac tctttcacca ggccctctgc ttagaatagc agcaccaaag cctggatata
20281 tgcaatggca atgccatccc agttttccat tcaacagtaa actccgcatt ccaaacaata
20341 ttggggccaa atagcattgc aactgcgaga taaaattcta aggagagttt aatactagac
20401 ctcagaacct ctgccaaggg catcctcttt ggagaggttg aggtccggag gattcccctc
20461 tggggcttcc cctagtgggg cccaatctta agagtgtcag acgtctctga ccttaggtgg
20521 gcaccagtcc tgcttgcatg ttttccctct aaagggaatg gcctactatg agctttcatt
20581 ttgtccctgg atgaaggcct tgacttttag catccttata atttgataag gccacacttt
20641 cccatgtttc ccattccatg aacttaataa ctggaggctg gatgtgtttc ttttgctctt
20701 agccagttga ctagggaaac ggaagaatta cgcataagaa aaggtttaag ttgcctgaaa
20761 caggcgagtt tgctctgagc tgtgccacac atagggatca gggaccacac ccagaaaaga
20821 tttaaaaaag agtccttctc ccttctgggc agggcaatta tccccattca ctccatggtc
20881 ttcaggcaat attggggatt gatcccagcc aatttccctc aatttccaaa gagctactag
20941 gaaacagctg ctaaaagacc aataaagaag aaaaggaaaa aaaaaaagaa aaagacccag
21001 gtcccttagg caaaccaggt ggtggcggtt aggcttcttc acatgaaaac cccttagtat
21061 tcaccagcca tggccagaaa cctgcagttt acctccatgt ttacacgctg ctcaccaagg
21121 gtcccgagtg ggaaaggaaa agagagagag agatttccct gtatgtaaaa agggaaagga
21181 gaaaaataaa tcccaaactt tgggcttacc ttctcctggc tggctcacca aaatatgtta
21241 ccagtggagg gtgtccaggt gcttggcatt ttgaacaaag aattggacaa aatgcacaaa
21301 caaagcaagg aaagaatgaa gtaacaaaag cagagattta ttgagtacac tccacagtgg
21361 gagcaggccc aagcagccac tcaagggccg ggatacagga tcttcttggg tccaaatacc
21421 cgctggaggt ttcccattgg ccacttggtg ttcacgccat gtaaataaag tggtggcccc
21481 caatcagtct gattgtttgc agacggaaac caatcagagg ctgaagtgaa gttacaaagt
21541 tacactctta ggcaaacgcc tggttgccaa aagcaaccaa tcagaggtat tttctttttc
21601 tttttctttt tttttctttt tgagacggag tctttctctg tcacccaggc tggcgtgcag
21661 tggcccgatc tcggctcact gcaagctcca cgtcccgggt tcacgccatt ctcctgcctc
21721 agcctcccga gtagctggga gtacaggtgc ccgccaccac gcccggctat ttttttgtat
21781 ttttagtaga gacagggttt caccatgttg gccaggatgg tctcgatctc ttgacctcgt
21841 gatccaccct cctcagcctc ccaaagtgct gggattacag gcgtgagcca ctgcgcctgg
21901 cccagaggta ctttcaatta accatctgcc atgcagaaaa ggtgggagtt tgcaaagaga
21961 gtagcctctg gtccttttgt tacttaggca tggaaagtta gggttttcct ttcaatgtag
22021 ttctacgaag tcagcgtgaa atggccttag gtttcctatt ctcctgcctc actggtatcc
22081 aagggactct gccattaggc tagctgtgga gggatggaga gagagctgag gcctcaaatg
22141 ccagtattcc ttccttccag atttgcgtgt aagaccagat aatcttccag accaaacagt
22201 atctgttgat gagtattagg ctcgaatgag aaggagagag aagtggagca ctggattgaa
22261 gagaaagcaa aaaaccttcc tagatcaaca aggaaatagc ctcttctaga atccagatag
22321 ggccctggac tcctgtggga taatgtagtg ggtgaaaaaa ttggtaRtga agtcctcgga
22381 tatggggatg cacttggaag tcttgggaac aaggaagaga gacatcctgt ggtggtgatt
22441 tttagatcca acacccaggg gcttgggtgc ctttgggggg attagtagaa gagatcgaca
22501 cattgaggga ggttcaggat aactgtctga aaatgagaga tttttattgc ttatactgat
22561 gtcccaatag ggtctgtatg tgcttcaaat aggtagaaaa gaatgaagtc cgagcctgaa
22621 gataacaata tcagtcagaa ttttaggatt gtgaatacct gtccacagaa atagctgggc
22681 cctgagcctc tgctgatgat gtaggaatRg aggcagttaa taagctttaa agtcataggg
22741 tctcagggct agggtatggt gaggacactt tgccacccct aaggttcaaa gcctcttgga
22801 ctggaggagg ttgttaacag atattctagg cagaaaagct gtgtcaggtt gtggtgtatg
22861 aagagggaga atttgacacc aaatggtcag ctggccatct gctcacctgt tcccctgtgc
22921 tccctactcc cacagggtct gcttacagaa caaggcacag caaggaggca agacacaagc
22981 aagaaatgtg cttgatgtta cccactgcag tccttcttcc actctggagg attctgaaga
23041 ctcagaagag ggacaggcaa tggattcagt ctaaacccca ggaccaggac actgcctcag
23101 cttcgaagcc tggatgcaat gctccagggt ataaccactg cccagaagca cagctagtac
23161 aagctgcagc ctcaatccag gcaaggagcc aactccaaca ggtcaggctc caagggatga
23221 agactgaaaa catgaatgtg ttgtgccact aactctagcg tttgcttttc cttgtgtttt
23281 tgcatgcatt gtagaccttg gcatcagcat ttggctagct ttaagacaga aactgagaag
23341 ccttttgtgt tttatgcgtc aacttgactg ggtcacagca tgcccaaata tttggttaaa
23401 cacgatttct aggtgcatct gtgaggattt tctggacgag actaatattt gaatttgaat
23461 cagtggacta agtaaggcag actgccctcc ccagtgtggg tggccctcat ccttctctct
23521 gcctgacact cttctagctg gaacatttgt cttctcctac cctcggactc agactatgac
23581 tggaattgat atcattgact ctcctagttc ttcagactca gattgctctc Scgggacttc
23641 tcagcttcca aatcacatga accaattcat tataggaaat tttttWatat atcctattaa
23701 ttctgtttct ctggagaacc ctgattaata cagatttatt ttcctacact ccagtaactc
23761 tccactactc cctccctcaa cccctggtaa ctatcctact ttctgtctct ataaatttga
23821 ctattctcag tacctcatat aagaggaatc atgcagtatt tgtccttttg tgactggctt
23881 atttcattag cataatgttt tcaaggttta ttcatgttgt agcatgtatc caaatttaat
23941 tcctttttga ggctgaatga tactccattg tatgtatata ccatattttg ttcattcatc
24001 cattgataaa catgttgttc ccacatcttg gctattagga ataatgttgc tgtgaacatg
24061 agtgtacaaa tatctgagtc cttgcctttt tttttttttt ttggacacag agtctctctc
24121 tgttgcccag actggagtaa agtggtgtga ttctcggctc acggcaacct ctgcctcctg
24181 ggttcaagtg attctcgtgc ctcagcctcc ccagtagctg ggattacagg cgtgtgccac
24241 catgcctggc taattttttg tatttttaat agagatggga ttcgtcatgt tgcctaggct
24301 ggtctcaaac tcctgagctc aggcaatttg cccacctcgg cctcctaagg tgctaggatt
24361 acaggcgtga gccaccatgc ctggatcatt gcttttcatt attttgggta tatacctaga
24421 agtgggattg ctggatcata tggtcattct gattttttga ggaaccatca tactgttctc
24481 cacagtgtct gatgacctac agttttgagc ttagcctttc taaaatagtg gggaaaaaaa
24541 tgaaaagaaa acaactagaa gtggaagtaa tttgtccttc attaagccag atatacacat
24601 cctgattcaa gcttgaaaac aatatcacct ccttcgctga tatgtaagta cctttttata
24661 atttaaattt taagttatga ttaattagaa acttaaaatg tatagtctgt atatgcactg
24721 gatgtccaca aagtgaccct tggttgctaa atctttgaga aaggctgagt agacactgaa
24781 cgaatgtata tccaacttac ttgtcaactc ctccaagaac attccttacc ccctgaccca
24841 agacatgctt tctatgagct ccaacagtgc ccctagtctt caacagctac cacatctgtg
24901 ctcgtcacac tcttgtcctt ctcctagtct caactgtgag ccatggatgg cagaggccgg
24961 tttttaccct tcaggtctcc agttaggcct gtgatggttc tagggttttg tttaggctgc
25021 gagtctgatt ggggctagga caaagggtca aattcaggat gaaaatcagc ctggccaaga
25081 gcagtggata catagccccc atcatcccac ttctcagatg ctcctagaac tccagcccgg
25141 gatcctttat cctctgtata ctccaccttc tccccataca agaccctctg cagagagcag
25201 gcacaaggag cagataaatg atggtcgatg ctgcctgtca attcagatgg gcctggcata
25261 acggttaact aatgggtttc ttgtttgatt gataggttct ctcaaaacta aaggagttaa
25321 atttctttaa tagcaataca tgctcattgt taaaaactca tatcaactga atttcccttg
25381 aacactatta acatttagtg tattatcctt tcacatttgt atagatattt agaaaaatgg
25441 agtgtagtat atacgtttat atatttttgc ttagcagtac atcatagaca tctatttcat
25501 tatattcaaa tggctcaata atattcccct gtgtagctgt accataatct agccaaactc
25561 ctatgtgggg gcctggtttg tatttgattt ttcagtaaYa taaacaatgc tgagataaac
25621 ctctatcagc tagattattt ctattgactg attttaatac atgattggaa ttttaaccac
25681 aagggggaag caggcaacca tgggaagaca ggctaagatt taggataaag aaaaagaggc
25741 tgggtgcagt ggctcatgcc tgtaatccca gcgctttggg agggtgaggc aggaggacag
25801 ctcaagccta ggagtttgag gttacacaaa gctataatca cactactgca ctccagcctg
25861 ggtgacagag caaaaccctc tctctcaaaa aaaaaaaaaa aaaaaaaaaa cgatttagaa
25921 agttatctca catacataag cttaccccaa ccttgtgagc aggtagtagc attagtatta
25981 gcctcatttt ttggaggcaa agctgatgat ttcattatca ggcgctcttc tgtatttcca
26041 aataaattga cagcagtaca acattctgcc tacattcagt tcaagtccag gtgctgtgct
26101 ggggacacta tgaggcatgc agaaataaag aagacaatcc ctgcccctca gggggtcatt
26161 atcaggtgtg aggacagaca cacctaaata accaagtgcc cactgtaaca gacaggagag
26221 cccaggaagg taggaaattc acttcgatag tgaggctggg cacagtttta tggaaggggt
26281 atgctgacaa taagaggtaa ggtgtattta gcctttactc tgtgcatggc actgtgctaa
26341 gtgctttacc tgcataatct cattaaaact tccccacagg ccaggcaccg tggtttatgc
26401 ctgtaatccc agcactttgg gaggccaaaa caagaggatt gcttaagccc aggagtttgc
26461 aaccagcctg ggcaacatag cagggccctg tctcttaaaa acaaaccaac caaccaacaa
26521 acaaaccaac aaacaaatcc ccatagacct ggaaggtatc ctaccctttt ttacagagaa
26581 gaaaactgat ggtcagagag gttaagaaac cagctaccgt ggcacaacac tggaacgcgc
26641 gtccttaatg tgaattcagg aggccctaca agggttggta gactttcaac aggcagggct
26701 gggagagaga cttctaagag aggaaacaca gaaacgaagc cttagaggca agaaatacag
26761 gcacgtctgg gaatggcaag gggatcagcg tgcctggggt gtgaaaaagg aggaatctga
26821 agagatcaaa actagagaaa gaaaactgag tcagagtggt gaagactgtg agtagcaagg
26881 caaggagatc ccgctgtatt ttgtattcag gtgaaaccaa ctgaagataa tgacatcatg
26941 tgtgctttat gaggattaat ttggtgcatt gcMtaagaga acaaggagac tagttagggg
27001 cttgttaaaa tagtcctggc caaagataac tagcgaagga atgagaacca gaagacatta
27061 cagagataaa atccacaaga tcttattaga tgctggggaa agaggaaaga attgtgatta
27121 agattttcat gtttgggcaa ccaggagaat gtgagtgtgt acaccaggag aggggctgga
27181 cagaagggag aggcgagttc acttttcttt tatgagctgg aaggaccaca gggacctttg
27241 agtggagatg tttagaggag agttagaaac aggaagctgg tgatttagac atagtacctg
27301 agaatcagaa gaaacccagg gccgggtgtg gtgtctcacg cctgtaatcc tagcactttg
27361 ggaggccaag atgggagggc tgcttgaggc caggagttca agaccagcct ggtcaacata
27421 gtaagaccct ctctatatag aataaaataa aatttaaaaa aagaagaagg ctgggtgcag
27481 tggctcacac ctgtaatccc agcactttag gaggccgagg cggtggatca cctgaggtca
27541 ggagttcaag accagcctga tcaacgtggt gaaaccccgt ctctactaaa aatacaaaaa
27601 ttagctgggt gtagtggcag gtgtctgtaa tcccacctac tcaggaggct gaggcaggag
27661 aatcacttga acccaagaga cagtgagcca agattgcgcc attgcattcc aacctgggtg
27721 acagaacgag actctgtctc aaaaaaataa aaaataaata aaaagaagaa gaaatgacca
27781 agggagctgg ccagggaaaa acctacactt aagattcagc aaggcatgtt agaccctgat
27841 aacctgcaat tccacagcca ggtttctatc ccagagaaac aaaatcacac gtgcacaaga
27901 gagcgtatga aagttcacag cagcattgtc tgtcttggtg aaagcctggg tcagggagga
27961 gcaggcaaat aaaccattaa acatccacat tgtgcaatag ttaccaatta agtagatcaa
28021 aggtattgac atggacagat cttcagaaca tacagtggat gagaaaaaca acatgtagtg
28081 caataataat gtataaaaca ttacttatga aaaaagaaga aaagccaaac aatcctgtca
28141 catatatatt ttaaatgatg tatgtgtagg catagagaaa attctggaaa gctaaatcca
28201 tgctaattgt gataaagggg aggagttggg acaaacatga agagcagaag gggttaaatg
28261 gggttttagc atagttgtca ggttttaatt ttataagaaa aatgtaatca tctgttactt
28321 atgtgaataa aaattctcaa aatattttta aaaagaaaga ttcaatggga agaggaagca
28381 atggaataga caggaataca ttcctagact ctaggtagga cactgttact gggaacactg
28441 ggagaagttt ctggaaKgca aggtggaaaa aggatccact gcactcagca acgctgcaga
28501 gacagcagcc agcaggatga gtcctgtgag ctcagctggt tgtcaacacc tttcacttac
28561 agccactagc tcctgtgtcc ttgaaaagtc gccatgtccc accactgccc ttgaatgaga
28621 tggagacttt ctgttctttt ttgagttgta aacatccaaa agcatttcRt caatttcttg
28681 tatttagaca ttcccttcct ttcccagaaa ttccacagtg atattctcag tgttctcttt
28741 ccagtccttc aagctgtgta tgaaatctac ttcttcccag tccagctcca gcaaatacct
28801 gcctcctctc ttgttctcca ttcaaaaaac ttccaatttg aggtcatttt taagagcatt
28861 actgtggtgg tggcggtggt ggagtttata gaatttctca tgggacccac agcatggatg
28921 tggctaacac tgactgagca cacaaaggtg caggcactgt gctaacttca taggaagtat
28981 ctcggttaat cctttcagca accccagtag gtcagtacta ttataacctc caaggtcttt
29041 ctttcacagc tgggacttag tgtggctgag cgacctaagg aggtaagtgg cagtaagctt
29101 gtggtgggtg actctgtgtt tgtctcacta tgtgagtttg tcctaagtga tcaatagggg
29161 tggggactgg actagaaaca aggtctcagg tatgcagggg tgttcagttt accaaatgcc
29221 tttatgacca ttaattcatt tgatgtcacc acagtgcttt aatgacatgg gtcattaggg
29281 aaaataatga gatgagtcaa gctttgtaat tcccttaaag tggtcaagtc tgggttaagg
29341 gcttttaggg cacattaact cactatgtcg tcttgagcaa ctctgcaagg ggtgggagca
29401 gagggggccc cctttggccc agctctctac aaatcatcta gttcctagca gccactcctc
29461 ctggtctgca aattcttggc gaggcaccct cctgctctcc aaacccaccc attccaggcg
29521 atcccttaag tggcagagga gccaggttcc tggggttaat aagcaggctg tttatagcca
29581 gcagcagtca cgggacagcc agctggaagg tcatgcccct tctgaatgtt gaggtttctc
29641 ataaacattt ccccagtgag ccttctggga gatgaggatg actctgggcc cattgccctt
29701 gctggggcag agcgccaagg ccccatactc actttcttct cctttctccc gaatctggct
29761 ctcttcctct ttcctccttc ctctcctact ctgctgtcat tccttccctt tcttcagtgg
29821 aaagaggaca cgtttcacag tagacacaca ggataactca accttctgag cctcagtttc
29881 ctcctgcgta aatggggtaa taatgggRcc ttcatctcag gatgtaaggg aaagtgctta
29941 gcactgtgtt aggcacgcac agtggacatt taaaacatat tggtcatgtc tttccccctc
30001 caccctatcc ttttgcccta atcctggcca ctgccctgtt ttctttctat tttcctgctc
30061 cagccctgct cccatttcca ccctgggcac ttccacccca ctgaYtgtgc tgctgctgcc
30121 tgggagggta gacgaagaag ccaggcttca ttcagaggag ggctgaccac cgcagggccc
30181 gcctggcctt ggcacttccc catgagaccg caccctaggc ctctctgctt ttagtaaata
30241 gcccatggga acagggggtg gagggaggat gtagaatggg agaggccaag acattttaac
30301 ctttggcttc tatttgccct taatctggct cttccaatgg cttaatgatc agagatcatt
30361 gtgtaaattg ctgtgtgact ttgggcaagt ccccttcact ctctgagctt cagtgaagtg
30421 tgggaaggaa gtgaagagca gaagttgggt agaagtgcag gtcctggaac cagaccatct
30481 ccaccacttg ctagcttgga gtacataact cctctgtgcc tcagettcct caactataaa
30541 atgcaattta tagtatacct aatttataaa agcactttta aaagtgctta gaatagcgcc
30601 tagcccatag ttggtactaa attaatctca gctattatat gcaaatggag aggtagaaca
30661 ggggtatgtg tgcatgagag gagagctgga ccttctctga gggtattcta Ragtcctgaa
30721 aacccacatt cctccttttt ggataagaag aagacagaac cttgaacaca aagtacttct
30781 cccaaggaga ccataggaaa cttgtcaaag acttttaaag ccagatggac cccttagagg
30841 tcatctggtc Yaacttgtca tgctacagta agaacagggt ggaccagaga gattcggact
30901 tatctgggac cacagagctt ttgacgagtt atagaattgc aggtggtgca ttgcagggaa
30961 gggaggcact ttaagaggac agtgggatgg gctgccagag ggctggtggg atgctagagg
31021 tcagattccc aggctccatg acctccagga ttcttagttc aggcacttac cccataacta
31081 aagcactgat gtcaccaccg gcatctttaa taattataac catgtaatga ggagggacta
31141 tatcccaagc actgcaccag acgctttcac atgcattact tactccatac aaaatcccct
31201 tgRcaaatga ggaaacacgc cttgcccaag ctgcaacaat tacaaagggg cagagctggg
31261 ctttgaatcc cggtctctct gatgccaaaa ccagggttct catgcaacac tcttttcatg
31321 tttcccagag cctagtttga ggcttgagcc tcaataaata aggatcttgt atttgtctca
31381 ctgggctggc caagcagatc ttgtatttgt ctgactgggc tggccaagcg ataacactaa
31441 tgaatMagcc tgggtcccag tcacctttcc agccctttcc accctgaccc acaagccatg
31501 atgagagacc agaccctgtg taggaaatct ttcttccagg ggccctgcct ctgtgttatc
31561 tgeccccact gtcaatggga gaagcagtgg ctgctttcgg aacaggcagg ggaggagggc
31621 ttagagcctg gggtgacRct tctggctgct gggcctgacc tccccagact ttagccaggg
31681 ccctgacttg aatgggagga gcatgaacaa acagaaagtt gtaggagaag caagaaacca
31741 ggtaaggtga aaaaaacttt tcccctccaa aaatacgaag tttatagttg atgtgaattt
31801 gtcaacttaa tatagtgtaa gaagcataag gtttaatacg gtcttcctcg ccatgatggc
31861 aggaagcaca tctgttgtat tcactaccac atgcttagtg ccccacccaa ggcctgtcta
31921 gagtcagtgc tcaataaata aacatggaaa atgaatgaaa aaaaaaaatg gagggacata
31981 aggaagagca ctgaaacatc agtcagaaaa cctgcattac agtttttagc ttcatggctc
32041 acagcaaccc ttggacaagt cacttcggct ctgtaaacct cagtttctac aagtgtaaaa
32101 tagatatcat ggcagttcct actgcacagg gctttgaaat cacagacttc cgtaggagtc
32161 atggttccat aaccaactaa tgtcaagttg aaaattgtat ttcttcagag tgaggccggc
32221 tttagcaatc tatgcaaggg gtcttgctga accagggcca gaggaaggcc agaggtgtgg
32281 ttctaagaat gaggattaaa catgagagag actataaatc tgcttggaga accgcagcat
32341 taggagggta cagcggacct ggcaggcatg acagatactc tctgccctgg agagtctcca
32401 gccctgccca tgcaaggtgt cagttagtat cagcgggagc acagatgggc ttaaatcaga
32461 agatgttaca gattatctag tccacactgg acaatagccc agtgaggggt taaaggctga
32521 gagacaaaga cccagatatt ctaattccca gctcaatgct actcttccca caccaccatc
32581 aaaggagggg gacggtggtg agagggggta tttgaagaga aacagattgg acaaaggaat
32641 gaatgcctcc atgtagcaac agcttcaaag gggcttcaga ataggagata tcttgttaaa
32701 gaaggaggaa cgagaggggg aaatggttaa aaataacgtc ttacatccat gtggattatg
32761 tttccaacta tatcatgtgt agttattgca tgtaaggtac tatggtggag agttactgat
32821 ggggcatcag aaagcccatt tcattaccca gctctgccat gtacaagctc tgcgacctcg
32881 ggcaaatcct ctctctgatc ctcaaatttt catgtgaagc atgaagcaat agtgcctacc
32941 ccatagacct atcctgggga ccaggttagg aaagggaagt ggagggaggt tgtatcctct
33001 ctaaaatgct ctgcaaatgg gcaacatggc aaagcggtca caagcaagcg tgtgaggtag
33061 accRtcactt agcctttctt ttcttttttt tttttaagac agagtttcgc tcttgttgcc
33121 caggctagag tgcaatggca cgatctcggc tcactgcaac ctccacctcc taggttcaag
33181 tgattttcct gcctcagcct cctgagtagc tgggattaca ggcgcacgcc accacacccg
33241 actaattttt gtatttttag tagagatggg gtttcaccat gttggccagg ctggtctcga
33301 actcctgaca tcaggtgatc cgcctgcctt ggccttccaa agtgctggga ttacaggcgt
33361 gagccaccac gcccagcccc acttagcctt tctacgactt tgtatcctca tctgtaaaat
33421 ggatgtggtg ggaatcaaat ataatgcatt tgcagccttt ggcacagtcc ctgacacata
33481 caaagcctgc aacatcctgt tagttactac cactagttgg gtttaaagaa ttttctcatg
33541 taatcctcac aataatccta tgaggtagga ttattattat tctcatattg cagatgggga
33601 aaccaagacc taaaaagaat tgcttaccca aatcaatagc taattagtag acagcaaagc
33661 caactagaac ccagatgccc tatcagatca ggcaggcaaa gatcagaatt agtgagtagg
33721 gcaggcagca gcgggaatgt ctgtggagtc cagacaataa tgcagccaca aagccagagt
33781 tctggtagct gcagaggcat ttccaacttt aaactctggt cagtctggag ctcagtcaga
33841 acaaaccttg tttcatcttt gatttaagga gctgagaaaa ataagctctg gaaagagaag
33901 aggggaccct gtgttccagc atcatctgcg ttatttattc actaggctga ttgcttcKcc
33961 tctctgggcc tcagtttccc cagctgtata tgaaaggttg aactagatta atgcttctca
34021 aacttgaatg aggttctgag tcacctgatg atcttgtttg aatgtagatt ctgattctca
34081 gtaggtctgg gaaaaagcct gagattctga atgtctgacg agctcccagg ccatgccagt
34141 gctgcagagc taaggatcac tctctgagga gcaaggaact agatcagctt gaaggacatt
34201 tctggtatga taatgcctat gagagcaata caccaaagac tgagaagtcg agcaaaaaca
34261 aattctgccc aaagtgcctt acagtatttg ggggtccagt gtgagggcca ggctatttcc
34321 catggaccac agaccccttt aaaaaggggc ttgatatcca gcactcagtg aggcattcag
34381 ctgcagggcc agctctccag actcttcttc ccagcccatc tcccaccctc acctgcactt
34441 cacctcctaa agggaagaga caaaattttc taagccattt ccctccccta tcagggctgg
34501 ggagggagcg gcttgttcct aatgaagaca aaggacaagg cttaagggac acttgagaat
34561 ctttttctgt ccttcctttt cctcattcat tcattcattc ttcattattc aactatttat
34621 tgagtactct ctacgtgcct ggtattctcc taggggctga ggttacagca gagaaaataa
34681 gacagatgtg gcctctgacc tcatgttgct tacattctaa ttgggaggaa aatgggacag
34741 gccataaata ggtagatttt aaaaatgaat gagatcatct aaaatcacaa aaagcaataa
34801 aagaataatg aaacaaagtg atggggtagg gggcttcttc tgtctattgt cctcaagaca
34861 aagtccaagg ttggaaaagc tcatctccac ctgcccttgt ttgcttctcc agcctcatct
34921 cctgtcactt tttgccttga actttccatt ccagccacac aggacatgga gtatcaaggc
34981 tggaagacgc caaccagcct ttgatccagt ggttctctca gccccaatca ctctttctct
35041 ttgtccctac ctcttcctct ttttccctct ttctcaccca actaacccct cctgctcttt
35101 cccagcttac tcgccactcc cactggaagc tggccctgct gccactgatc tgggctagga
35161 atccgtctgc gccaccttcc cWgatgacag catccatcac ctgtgcagcc gtgttaattg
35221 gctgtccttc ccccaccaga ctgtaagtgc ctcaagggca ggagtcctgt cttattcact
35281 ctgaatcctg aatcctcagc agttgctgca atgcaaagca cagagtaggt gcttaaggaa
35341 cacctgttga attaactgac actaagttta gtatatttaa gagctcagta atgggctggg
35401 cgcagtggct cacgcctgta atcccagcac tttgggaggc caaggtgggc agatcacctg
35461 aggtcaggag ttcaagacca gcctggctaa catggtgaaa gcccgtctct attaaaaata
35521 caaaaaatta gctgggtgtg gtggcaggtg cctgtaattc cagctactcg ggaggctgag
35581 gcaggagaaa cgcttgaacc caggaggcgg aggttgcagt gagctgagat agcaccactg
35641 cactccagcc tgggcgacaa gagcaaaact ctgtctcaaa aaaaaaaaaa aaaaaaaaag
35701 ctcagtaaca tgagacaaag gcatctacaa gacatggctt tggagacaaa gaataaattg
35761 ataaattgga aggaaaagaa tataacaatt cctcagtcct ttctggagtc cacattctag
35821 tggctttcag ctctacactg tatcaggcct cctacaaggc ttaccaggca gactgggcag
35881 aaattattga cccccattca acagctgggg aaggtcatgc ctcagagagg tgaacctact
35941 tcagctgaag ttacccagcc aggtggtggc aaagcatgtc caaagaaagt tagggaccag
36001 agacgtcaga tccaacccct ccctcttgca ggggatgggg taggggtggt aatctgcaga
36061 ctctgaacta agtgttctag ctcatttcaa actcagaata accatttgag gtaaacagta
36121 gccatagcca caaaaaaagt agacacatga tttgcccatc atccaccttc cccctctaac
36181 ccaattgtcc ctgggcagac cgtgcagagt accaccccat tcgtcctcaa ggactccctg
36241 cctggggtca actcatgaga aactgtccac ccgcagattg actgggacag aaaaaaccct
36301 agacttttaa tttccttatt tcacagttgg agacaatcca aagcttcgag agaaggaact
36361 agtctatata gcacatactg agacagggtc agaactccgg agtcagaact ttctgaagta
36421 tagcccacaa gtccccttga catgaacctt ttgagttggg aaagggctaa gaatgatgtg
36481 aacatgacct ggccatttgt tagctgtggt caggctgggc gaagagaggc agcccctctg
36541 gtagttctac tctagaactc attagtcccg ccagtcctca atggtttgtg cattggtagg
36601 tagcccatgg gaagtaggat gtgggttgct tactgcttag tgactgttta tgaagattgt
36661 tattgctact acagcagtat agtctcccag ctgaacaata acctagaaag tatggagagt
36721 ggccgggcgc agtggctcac ccttgtaatc ccagcacttt gggaggccga ggcaggcgaa
36781 tcacgaagtc aggagttcga gaccagcctg gccaacatgg tgaaaccccg tctctactaa
36841 aaatacaaaa aattagctgg gcatggtggt ggtgcctata atcccagcta ctcaaagggc
36901 tgaggcagga gaatcgcttg aacctgggat gcggaggttg cggtgagctg agatcatgcc
36961 attgcattcc agcctgggag acagtgcgag acttcgtcgc aaaaaaaaaa aaaagaaaga
37021 aagtatggag agtaggtgga gaggggctag cagagacata ggtagggcac cttaccacta
37081 agaaatagag caaagggtag gtgctttatg ttccagaagc atatagatcc aagaggaggg
37141 aagggacatt ttggctgaaa gcttggaact atatctagca tagataaatg tgattctcat
37201 aggtgcttgt atttgctatg ggaccctgga ccaatcattc agtctcttga agccttagcc
37261 tctcccataa gagggtggaa tgagctagtc tctagggatt cttcttttta taactctccc
37321 tctaagtggg ctttgcaggc agagggagtc attaactcct gctatggtct gagtgttccc
37381 cctcccgcat attccgatat tgactttttt tttttaagag tctcactctg tcttccaggc
37441 tggagtgcag tggcacgatc tcagctcact gcgacctcca actccctggt tcaagcagtt
37501 ctcctgcctc agcctcctga gtagctggga ctacaggcgc acaccaccat gcctggctaa
37561 tttttgtatt tttagtagag atggggtttc accatgttgg tcaggctggt ctggaactcc
37621 tgacctcatg atccgcctgc ctcggcctac taaagtgctg ggaatacagg cgtgagccac
37681 actgcgcctg gctatattga catcttaacc cccaagtgat gctattagga ggtgggaact
37741 tgggaggtga ttaggtcata aaggtggatg gagcactcat gaatggaata aatgccctta
37801 taaaaaaggc ccaagaaagg cccctcaccc catctaccat gtgagggtac agtgagaaga
37861 cagccatgta tgaggaagca ggtcctcacc tgatcccaaa tccttgatct tggacttccc
37921 agcctgagaa ctgtaagaaa taaatctgtt atttacatgc cacccagttt atagaatttt
37981 attatagcag cccaaacgga ttaagacaac tccatttcag gtaagcccaa caaacctagt
38041 atttgccagg ccaggtcctg ttgaggctta attgagaaat gtgtagcaaa ttgttagtag
38101 tacaggcccg agaccatgat ccttagaccg ctacacttgg cctgtggtca gctcttggga
38161 acttctattt cagaaggatt tccaccattc cccaaactaa taagagtagc tcattatgcc
38221 taaactggac aaacaatgca gtttctgttg actgcctaat tttcttctgg gagtttagaa
38281 ttctggtatg tgcttagcca aggatgccta tgtgaccagc ccctaacaaa aNcctggcat
38341 tgagtctcca gtgaacttcc ctggtaaaca acattttaca catgctgtta cagttcattg
38401 acaaaggaat gaagcacatc ctgtgtgctt aattcagctg ggggatgatt ctgggaatct
38461 cgtggctgtt ttcttccaga ctttgtccca tgcacctttt ccctttgctg attttgtaac
38521 cttgtgctgt aataaatcat agtgtcagtg tacttatatg ctgagttctg tgagtcctcc
38581 tagcaaatca ctgaacctag aggtgatctg gggaccccca acaggagtta agcctagatt
38641 tcacagagtc aatcataatc ttgtgccagt cccttaagag tatacagctg aaaccagtgc
38701 tatccaacag agatataatg tgagtcacat gtaattttac attttctagt ggctatattt
38761 taaaaaccaa aaagaaacag gcaaaactaa ttttaagcac atgttttctt caacccaata
38821 tattattatt cttttatttt tattttcgag acagagtctc actctgtcac ccaggctgga
38881 gtgcagtggt acaatcacca ctcactgcag cctcaatctc ctgggctcca gtgatttgcc
38941 cagctaattt tgaaaatttt tgtagagatg tggtctcact atgttgccca ggctggtctc
39001 aaactcctgg gctcaagcca tcctcttacc tcagcctccc aaagcgctgg gattacaggc
39061 gtgagccatt atgctcagct aacccactat attaaaaata tatttaaaat atttccatat
39121 gcaattttat taaattaaga tattttatat tcttttatca cactaagtct tcagaaaaca
39181 ctgtgtgttt tatactcaca gcacagatca gtttgtacta gccctactcc aagcgtgcaa
39241 cagccacaca cgcccatggc ctgcctgcta tattggctta gaggttaatg gcaagggctc
39301 tggagacagt atatgcggag gctaaagaca ctctgtctag gatgctgatt caaccatgtt
39361 gatttctgat taactccagt tccaggaaag ccgctaaggt tttcagttta tctactcttc
39421 cttgtgtaag agcatgtcct taacataaat attcccctta ggtgaaaaca aacttaatat
39481 tctcacactt cagttggcct acacatccct tcaataggga aatcgcccct tccctatgct
39541 atataagccc tgggctgagg aggggtaata gcttggggat ccactatctc acttcacagc
39601 cacctaagac ttctgtggct tctgtttcta agtccctaat aaatgtttct ttctgagaaa
39661 ctgggtatgt cagcttcttt cttcagcttc tcagcctcct ctgactggct ggcgcggggg
39721 tggggtaggt ttacagagat ctgctcacag cagaatagta tgtgtgggtc agaatcccgg
39781 ttctgtgact taccaactgc ataaccttaa gcaaagttgc ttaacctctc tatgtattag
39841 tttcttcatt tacaaaatgg ggataataat gttggctacc tcaagaggtt gcagagatta
39901 attaatctaa ggcagcacct ggtatagata ggatattgag tgaatgctgg ctgctactct
39961 ctttatgcta aagcttggcc catggagatc aacttgtcta acacctcgtt ccataaatga
40021 gaaaactgca gcccagagtt tggaaggcac tagtttcaca agcaaaaact acagccaccc
40081 acctctcctc ttgatgaact ccctgtcttc tggctggccc tgagatcaaa tttgccaatt
40141 atttaggaaa ttggcagctc cagggcccca gctcggttct gcaatttctc tcgattgcaa
40201 aacaagggct actgtttgaa gttggcactg aagtgacgca ggctgggcct cacttctgct
40261 tccacctagg ctcatcagtt agagtggctg atcccatctt tgagttcaga acctgaaatg
40321 tttactaacc aataataagg ctggctttcg ttgttttaac tttttacaat cctttgagtt
40381 tattaatttg tgttctatct tccttgaaga cacagtgcat cagcactttt tgtaaaacct
40441 ctttttaagt catcttcggt ctctgttcac ctaccaagta ggtaagtagt gagctcccta
40501 tagaggtctc taaaaaatag gatgaagagg ttccgagtgc tcacttgtct ggaatgcgga
40561 agacgtgttc tcagactgga agcaaaacta gcttatgatt cttctacagc ttctccttcc
40621 atccctccct tcccattttg gccaatgcaa atcatctact ctcctctgac aagcctactc
40681 atcataaccc gcacgtcttg aatcttccta gaagatgaaa tcagaccagt cttgcgggct
40741 ctacatggtt acaacttgct aacttgaagc tctcattatt ctatatgcca aatattgttt
40801 tccttgacat taatttattt gatagatatg tatcaagcaa ctactatgta ttaagctctg
40861 tgctggtgct ggtgYctttt tggatcttat aatttagtgc agaaaataga caaacaagaa
40921 atgatgataa agtacaacaa agggtcgatg tacaaagtgt caaatactct aggaactaca
40981 ggaaaacaag ggaaccatca cgaaacccct gtcttaagac gtgacattag tggagatatg
41041 aaaaatgagt aggatagtac cagttaaagg gagacaacct ctgttccaag cacagggaac
41101 agcagagatg aaacctggaa gtgagggaga atggcagact gcaagaacgg aaaaatgtcc
41161 agtgtagcgg aggcagagag aatatggaga gtgtggcaaa agaggaagaa gcagatggat
41221 tttgagggca ctttctgaag tgctaaggaa tttagacttt aacctgaggg caatcagaag
41281 cagcctggtg gagaacgata tatttcaaaa catagggtcc ttgaaaacaa gtcaaccaaa
41341 tgtatttaaa aggctgctga tgaaaagatt gggaagtatt gttttcattc tcattgtttt
41401 cactgtcatg ctctttgtgc caatggttag acctcctttg aaagctcact gcagcatgaa
41461 agcccttctt aatgccaggg caggctcccc aagcctcccc accccactgt ccatgtcatc
41521 agttttgtac tcctgttttg tatggctcag gctttcctta tactcttagg aatgaaaagc
41581 cacagacacc accactgaga gactgtcaaa gatccttgtt aaaYaggtgg acaactgaga
41641 tcagagaagg aaagtaattt tattactttc Ygtagcaatc caggggtagc atctcaggtc
41701 tctggctcct tcttgtctcc cctaaagaaa tagaccacca gggctgggat taaggtgaga
41761 caagtgagac atagagggtg caaaatgtag agagatgccc gctgccaggt gccagcctgc
41821 acttRtgtga ccctaggtgc cttcccttcc ctcaccctgc tggtatctgc tggaaacacc
41881 tttgagagca gggactttgg cttttagaca actctttagc atctctcatg gtgttaacat
41941 gttgctaggt gcctattttg agggtcacta gatgcctact gatactaatg aacaatggat
42001 ttctgacaaa ctgatgtgca cacagatgtt tatattttag ggtaaatacg aaccttaaaa
42061 gacaacagaa caacatggag agggcctgag actgagtcta gagacctgga ttctggttcc
42121 ctccctgcca tcagttggtt atgggaactt ggataaattg ctcctcatct ctaggcttca
42181 gggaaatgag gtggttgaat aaaacgtatt gagcacttac tgtgtgtcag ccattgttca
42241 tatgctccca ggctttaact cagctaaaga cttcctggcc tctgtggttc cttccacttc
42301 aacaggctgt ggtttgaggt gggaagactt cgtagagaat gcaaaacttg agagacatct
42361 tccaaattcc cagggggatg ttaatacctt taatcatagt aaaggtatgg tttctcaagg
42421 catttcttgg tcagaagaaa gttccagaag gcacttaatc ctaggactca cactaccaag
42481 gctacagcca ggccaactgc ctcacaataa ccttagaaca tgtctgaaga cagattttca
42541 gcttctgccc ctagagattc taatgcagtg attccaaagt ggggtccagg aatctgtatt
42601 aaaggcttcc cagttgactc tggtggtcag agagatttga gactgattcc atcacattcc
42661 ccatctgtga acgggatcat acctagacag cccagcccta gaactgcctg gtctgatcct
42721 aagtgttcca tggagatttt gtttttttaa tgtaatgaat gattaagtca gcaaatactt
42781 tacatctctt gtgtgctagg ccctgtgcca ggtggtgatt atgggcccta ttctcaaagg
42841 actcctggtc tccgtctgtc acttacttta gaaacaagtc atctagccag agccagaagg
42901 gcactggaga tcactaatcM atcccatcat tacaYagctg aggaaactga ggcccagaga
42961 gtaactgcaa catgttaaag actacacagg gagcaaattt Wttttctgct tYtaagccag
43021 gtggtttggc cacagatagg ccaagaagaa tacgaacaga caagccttgc tgggtttccc
43081 actcagttta ttactattag accattccct ttttccccac tcacatttct gcgcatttcc
43141 atggtcttca tagaatctaa gcctaaaaat agagttttcc ttgagtcttt gggtcttcat
43201 ttYtgaagac tctcatgtca cagaaaactt taattaaata aatcgttatg cttttctctt
43261 gctaaccttt ttctacaggc atgtcagctg tgaYYcttat gatggggagg aaaggcaccc
43321 caccttctct gtccctacag tccYaatgcc ctacccagaa tggggttttc aaaatcccag
43381 gacacccact ctttgtttct taagcactaa acctatatat agtaaatgtt tctggaagtt
43441 taacttcagc tcttgctgaa attttaaacc ctcaatttcc agaggaccat cgccaggtgt
43501 ttttaaaaYc tcgataggaa taagaaatgc ttcccagaat ggagacttSc atcagttctW
43561 gggagctatt agcccccWtg ccctggctgt aatggagacc gctgtgtctg cctcctttcg
43621 cactttggag ccaaaagagR aagggaccgc ctcccacgtc cacagggacc tgacttccac
43681 ctctctgccc agatttgctt atgtcactgt cgccccggga cggggaggtg gggagctgag
43741 ggcaagtcgc gcccgcccct gaaatcccag ccgcctagcg attggctgca aSggtcYcgg
43801 cttggccgcg gattggtcac acccgagggc ttgaaaggtg gctgggagcg ccggacacct
43861 cagacggacg gtggccaggg atcaggcagc ggctcaggcg accctgagtg tgcccccacc
43921 ccgccatggc ccggctgctg caggcgtcct gcctgctttc cctSctcctg gccggcttcg
43981 tctcgcagag ccggggacaa gagaagtcga aggtgagtga gcctccgggc cgggggccgg
44041 gagaaaaaac ctagcccctc ggtgtccagc gctcagtgca atgcacccct tttcccaggc
44101 tccccgccag atgggcaatc cccaggtgcg agagacctcc tgaacccctt ttgccgcccc
44161 ctccgccgcc gggaccccgc ccccgaccgt cgtcgtctcg tagttccatc tgttggagag
44221 ccgagacctg gtgcttcagg cgggcagaat gactaaggga ggaaggtctc tctccccgag
44281 ctcgcacttt ctccccRctg ccacctcgag Rgtcgccttg ctacatctat gtcacctccg
44341 tgcctgagct gctccccttd agtagggcct aagagggagg gcgtcacaga aaaaaatgcc
44401 cagcgtctgg gtgggctgtg caagttccca gggagagaga caaggagaaa ggagagaggc
44461 agctgggtgg tcctggtcct gaagaactgc tgtggggggc tcttctaccc caagaatgat
44521 acaggcaggt agaaatggcc acttggattc agggcattgg tagaagaggc agagatctgt
44581 gctagaaRga gctctggagt gacagtgcag aacactcagg accctgagtc cttacctgct
44641 cctggacccc cgtttctcca tctgcagggg agagtttggg ggtgggagtg catggggcac
44701 aatgaagctg agggcctcag aggggatgct tggagaagct gaaaaatcca caagctgttc
44761 tctgaaattt cctcYcctgg ggcagcctca ctacccagga cccactgtcc tcattagcct
44821 gaggaaacct ggtagttgga gaaagctggg gactgctaat tcagaagaag aggtagaatt
44881 ctctagagcc tcagtctatt tccagcaggt gtgtggccca Yacctggagt ggggatactg
44941 aggccaggag tgggatgacc cagcatgctt atctcctttg acagtatgta ctggggtggg
45001 ctctgYagac ctctcctcta cccagggagt gggagtgggt ggggaaggag gcagaagcca
45061 ggtgtaggga tagactcaaa gcgagcgatt tctgtctYca acttcccaac atgcataact
45121 cacatttttg caagctctga gcttttgttt ctaaaatcct ctgggtcttc actttaatta
45181 agttSttatg agtctaggat tccgtttcca aaattctgtc cacttctcag gaagcccatt
45241 ggaagcacag ggttccttcc atgttactca ccccttttcg gtcctcagcc taaatccgat
45301 taatcagcct ctccttggag tcagtctaaa tggatgtgaa gccacttcgt ccaagtcccc
45361 tcctttttat ccatccctgt tcataatccc caactcagaa ggcattttcc aggtcaggaa
45421 tgggatcgat tttcaacatt gaggtttggg gtgggccata gacaggccag caccRtggag
45481 tcagtcccaa ccttcagttt tggaaRggtc aaagaagata caattgggcc ctgcctcaag
45541 aaSgctagtg gggagaacgt ggactccatg aataactcat catagctgtg tcttctctgc
45601 Mgcactctgg ggaaggataa atggactcag agagaatagg atggggatgg tYtagaaaag
45661 ttttagggtg aggtgtgatc atatcacata caaccagtca ggacttgctg gctaatgaaa
45721 gcctgggcat gcccatctct gctcccaagt tcttaNagtt cagatgtctt agctctgggt
45781 taggacccaa ccactcacct tacccccaac cccagggtaa agagaYaggg ttatggggca
45841 tgtggagaat ggaatggacg aaaggtgact cagccccaga gaggtgtttt cctcctacca
45901 attgaaaggg agatgctagg gaagagacca ggtcctcctc ttcttgctgc gtcacctccc
45961 cagtccaact tcaSgctgcc cacgctcctt gtctctacaa aaggactgca gaagccggaa
46021 agagtgacaa ctgctgacgt gcaYgggatc ctagtaactg ctggtttcta ggtgactaaa
46081 cctggcagag agaatcatgg aaccacaggc tgacagagtt aaagggccct taagacagcc
46141 ctgaacctga ctccatctta caatggagga gctggagccc cagcaaaggg ggtcacatgS
46201 catcagagac acagcaaRgc Ycagcactca ggtctcctga ctcccagacc acttctcaca
46261 gcatcatatt gataggagtR ataagacggg agaggagaaa gacacaagac cagcagcccc
46321 agccagccag agtcctgNct gagtcccatc atccaactct caagccattg gaaccctctt
46381 ctcttgcccg gctgtgggag gattcaggca ccctcatggc ctctcccctt atctcctctg
46441 gccactgttt cagatgcggt gctctgggat tccacgtgcc cagagaaggg aagggggctc
46501 atagaggact gagttgcagt gatcagtggt gagcacagcc gtggctctcc acccatgggc
46561 caccttctga cccccactat ccYttgacat gagatttcgt tccaagagac tccaggcctg
46621 ttttgctctg ccagcagatt cccacaaccR ttttgggcag cagcatgctt atctccctgg
46681 acatatgccc agacagtgta agtgacttgc tcagggccac Rcagccagca aaaaatagaa
46741 cctaatgaaa tagggttctc cttgccacac ccccaagctg aaactgtgcc cagaagctgg
46801 tccttctStt tacaagttcc cattattagg acaacctgta atcttttttt cctccatgtc
46861 tcagagttca tttttggatc atggcagagt taaaaaagtg gagaggctat agacatgcct
46921 ggctttctgg ccactagatc tttgtggcgg tctagggtgt atttgttgtg ccccagtttg
46981 tctgcatttt aaggcggaac tggccctggc tgggaacgag gaggtccagg agcagtagga
47041 atgttgatct taggtcttgg gcctgcctcc agcacccacc aaaagtcacc ggatgtggaa
47101 tattagactc atgtaaagca ggaagtttgc accagagcca agaagagagc ttgcagctgc
47161 cagggaatgg cctggcggag gaagcgcagc ctcgcccagc tcagcagacc tgcttcatga
47221 cagaggcagc tcttctgcac tMtactgggg ccacgccctg ccagcatata tcactccctt
47281 cacctggtca ggcccaggta ccttgcctca acagcaagtt ctccagaatt gaggggaaga
47341 gagggagtga atttgcttct ctaactccag ccacctgtct ctgccctctc ctggtctata
47401 atgctacttt gcattccatt tgtttattgt ctgcccacct ctcaaaggat atcagggcca
47461 caaaggacct taaagaccac cttatacaat ggtttcaagg tttctccttt cYcatcacga
47521 ccagcccacc tgccaaattg tgtcaatgat gcaatgacat cctttcaaga atagtctcag
47581 aattttgttg tctgtgtcaY gggcagagta ggctcatagg ccacatccta ctagccagca
47641 gtgaccccaa tcccctctct cccactgatc atgatcctat caagccatca tggttgacct
47701 ggttgRagag ttggttcagt tcaaggttct cattttataa atgaggaaac tcaggtccga
47761 agaaaagaaa gggccagtcc aagaccacac agggagttaa tggcaRaggg acatggagcc
47821 caggtctccc agcccacagt ccactgcact ctgtccctcc ttgtcctgga ggctgactgt
47881 gagcagcagc tggggaggga ggaaggcata agggtcatca ccagcctgct cagacccgga
47941 acattaacac atgcaggtac tgttgctttt ccctaccKca ccacagccct ttcccccatg
48001 cctggctctc agtcctgctt YcctRtttgg taacaacagc ttcatctttc ctgtcctgca
48061 gattgggagc ctctgaatca ccatgtccat acKgcctctt cctcctttct gttcagtttt
48121 tccttcacac tggcctagtt caggttctca tcctcaattg cctggattat tccaacagcc
48181 acccaaccag tctccatcag cccgtctagg aacccagtca tcagctttag ggcagtctcc
48241 ctgagagccc agtaaatgtt ctgaaattga catgtgatgt ggaagtaatt agcaaaataa
48301 gtcattctcc agggttccat agcactcact tattaagtgc ttaatattaa taagtgctta
48361 ttaggtgctt aatattaata agtgcttttt aagcacttaa tattaagtac ccRaaaagca
48421 cttattaagt gctttcataa tcattatctt aaacaaggaa ttaggagacg tcagtgctag
48481 tccagttcca attctcggtg attactttga gaaagtcact ctctctgttg ggatctttca
48541 ttcttttgaa aacggagatg tccaagttcc tttccagctc taactgctcc ttttatggcc
48601 tgtgttccag atggactgcc atggtggcat aagtggcacc atttacgagt acggagccct
48661 caccattgat ggggaggagt acatcccctt caagcagtat gctggcaaat acgtcctctt
48721 tgtcaacgtg gccagctact gaggcctgac gggccagtac attggtaaga gcccaccctt
48781 cctccctgct ttatttgggg ctgtatggca tatttcaatc acaggagctt ttctggtgca
48841 tgggggaaag ggtgatggca atcacgagag tccaagcccc ttttctcagc tccactgtgt
48901 tccgtggttt tgtgaagatg attatataag ccYgaggtct gattgccttt ggacatgttc
48961 taggagattc ctagttatcc ttcttcatct ctgggcacct caacaaccct aaaggcagag
49021 ggatagagat taggtttgtg cttagagctc cctttggctg gagcatgaga tggtaactga
49081 agctccatct tgctgaSaat atctYatttt ccctcagccc catctgcttt ggtgcttctc
49141 ttggcagctc tctggaaagc aggcaattgc ctcgaggccc cagagtgtat gcgtggtgta
49201 gcgggatgaa ggagcaggct acacttctaa tgcccattct aaaataaact ttgaagatga
49261 tctagtttag ccttcccctc ttctcaatct acacatggga aaccaagact cagagcgaag
49321 atacaactta aacagggtca caaagctatt agcggtggaa tggggcattt ggcgcaggtt
49381 tcatgatctc ctgatatgct tactcctttc tccctagctg gggtagaata cagaggctag
49441 gggaacaggc aaccaaaggc aagcgacagc acccagggga aaacgctttg gggctgagca
49501 gtctactggc agggggtcag ggaggcaagg gcaatttgac cctccatgct ctgcctggca
49561 atcacaggcR aattcccgga agtgaaagga Yggcggtcac gtggcccagt ggctgtgagt
49621 gctttttctg ggatgcttct ctctagcctt ggaacagagc agagtgcgtt tgggcaagag
49681 atgggctggg tgctgctgca aaaSWgacca ggggacactg aggaaactgg ggagctgggc
49741 cagggcctca tctgtgttcc tgatttgcca aagattatgg gagggttgta acctcacYcc
49801 aaagaagttc tctgccttgt atccagggat ggatagttct tgctccagaa aactcaatcc
49861 tgagggttct gaatgagtgc tctagctggc ccaggtggag ggccttggca atgggcaggt
49921 gactgacagc tgacttgagg aagggtatta ttcttgtcct ccaaacctcc aatccatcca
49981 gattagagtc aagaggatag gacctgagtc ccagcattgc tgtgaactca ctggtgatcc
50041 tgcgcaagtc cctccctttc tcgggcctca gtagttccag cRgcacagcg ggtgagccgg
50101 gggagtggtg tggatgagac agggctcctc gccaggatac tcccacRtct gctctttctc
50161 tttggcccag aactgaatgc actacaggaa gagcttgcac cattcggtct ggtcattctg
50221 ggctttccct gcaaccaatt tggaaaacag gaaccaggag agaactcaga gatccttcct
50281 accctcaagt gagtactcac tcagcatcct gagaaagctc ctctcacatg gcccacatct
50341 tgttatcaac cccaaWtcat ggtggacatt tatcggccac caagaactac tctcctcttc
50401 taggatcccc agtggaatga gggaagggaa gggacaagag agggagaagg acagggacaa
50461 ctggttgtga tgtgcatccg cagggagcac caaggttgag ggacactgaa aagggaccag
50521 gctagaaagg aagaccgtgg actcacatta tgcctatgcc cactgcacat tcactggctc
50581 ctgctgccca ctgcagaata aatccagact cccaacaccc tctcccctgt tctgtccctt
50641 cctctcattt ctgagccctg tgcccacctc cttgggaccc acctaagaac atttctcaac
50701 aggtatgtcc gaccaggtgg aggcYttgtc cctaatttcc agctctttga gaaaggggat
50761 gtcaatggag agaaagagca gaaattctac actttcctaa aggtaagtga gctgccacct
50821 gtgctggctg gggctgcagc ccctcctggc tccagcccac agcgtcaggg cccatgccac
50881 ctcccctgct cctgggctct tggggaattt cttggcacct gactattgtt ccaactagag
50941 ggctctgcag accctgacta gggtctcMtt ggcccatttt acagaaaggc ccagaaggac
51001 ccagagtgaa catactaagg gtctcacaMt cttctagagc cacagctggc gctggcagtc
51061 ttctaactcc caaactgggg ctcttYtctc agggccaggc tattccccag gaaggcctgg
51121 gaaggaagag ggtcaggggg cctcaagcaa ggttgacact cctctYatcc ctgctctaga
51181 actcctgtcc tcccacctcg gagctcctgg gtacatctga ccgcctcttc tgggaaccca
51241 tgaaggttca cgacatccgc tggaactttg agaagttcct ggtggggcca gatggtatac
51301 ccatcatgcg ctggcaccac cggaccacgg tcagcaacgt caagatggac atcctgtcct
51361 acatgaggcg gcaggcagcc ctgggggtca agaggaagta actgaaggcc gtctcatccc
51421 atgtccacca tgtaggggag ggactttgtt caggaagaaa tccgtgtctc caaccacact
51481 atctacccat caYagacccc tttcctatca ctcaaggccc cagcctggca caaatggaKg
51541 catacagttc tgtgtactgc cRggcatgtg ggtgtgggtg catgtgggtg tttacacaca
51601 tgcctacagg tatgcgtgat tgtgtgtgtg tgcatgggtg tacaRccaYR tgtctRYcta
51661 tgtgtctttc tgggaatgtg taccatctgt gtgcctgcag ctgtgtagtg ctggacagtg
51721 acaacccttt ctctccagtt ctccactcca atgataatag ttcacttaca cctaaaccca
51781 aaggaaaaac cagctctagg tccaattgtt ctgctctaac tgatacctca accttggggc
51841 cagcatctcc cactgcctcc aaatattagt aactatgact gacgtcccca gaagtttctg
51901 ggtctaccac actccccaac cccccactcc tacttcctga agggccctcc caaggctaca
51961 tccccacccc acagttctcc ctgagagaga tcaacctccc tgagatcaRc caaggcagat
52021 gtgacagcaa gggccacgga ccccatggca ggggtggcgt cttcatgagg gaggggccca
52081 aagcccttgt gggcggacct cccctgagcc tgtctgaggg gccagccctt agtgcattca
52141 ggMtaaggcc cctgggcagg gatgccaccc ctgctccttc ggaggacgtg ccctcacccc
52201 tcactggtcc actggcttga gactcacccc gtctgcccag taaaagcctt tctgcagcag
52261 ctgagcctac tgtgtgtggt gcttcttcaa tggtggccgc ccccgcctgg gtgggaagtg
52321 aggaggagaa ggtggggcag ggtaaaggSg ggaggatgag aaaagaacaa atatgacaac
52381 aaaaactttt tacagtgggt gcaaaggtaa ttgcggtttt Ygccatatgg caaaatccaa
52441 ggctacctcc ccaccccaca attctccctg agatcagcca aggcagactg caagggccat
52501 gtagccctat taggggtggc atcttcatga gRgaggcccc cctgccacaa aaaaggggca
52561 aaaattgcaa ttactttttc aacaacctga tagttagctg cagtggcgca ctccttcagc
52621 tacttgggag actgatgtgg gaggctccct tgagcccggg agtttgaggc tatagtgtgc
52681 cRtgactgag tctgtgaaga gccactgcac tccagcctgg gcaacacagt cagactgtct
52741 cttaaaaaaa aaaaaaaaaa aagagagaaa ttagacaatg ctttgagtcc ctgctttttg
52801 gctcctacct tggcccactc tgcttctctc actcagcatt tggagtttat aaaaccagtt
52861 ctgatctact ctcattccct cccacagttc tccaagtcag ctgaggttaa gatgaggtgt
52921 tctgtttgac ggatgaggaa actgaggctc acagaagggc cacgccttgc cccaggtctc
52981 ctgatgagtg aggggcagga ctggaattta aacccaagtc ccctgagtcc tggcctaggc
53041 tccttccact gccccggYtg ccctaccctc agggaggccc tgatacgtca ccattactca
53101 agagtaatgg tgaccatctc agggaaagga agaaaagggg aacagaagga gggacccagg
53161 aaagcagggg gttgacaggg gaacatYagc gcataaatga acatgagtca gaatgttgat
53221 cttcttcaac ttggatttat gtccatgatt cgtgcaaata gctatccagg gatggacagc
53281 caccctaatc tgggcttctg gcaccacagg cctccagcta tggggtccag ggtctgaacc
53341 tcagggcctg gcagcttcag gctggcgccc ctcggcRgac gtggctggca tggccttctc
53401 ccatctgtga tggcttcagc aaggctactg tgagtggtgg tggagagggc cccagagcca
53461 gaatatccat cattctcttc tgttctggag acaaacccac acttcccaca gctcctcaca
53521 gaggggtagg ggtgtgcgtg gggcaggtcc tgctacagag cttgaagcaa aaattaaaca
53581 cacacacaaa ttggtcatgg caactagagg gcctgaaacc acttccggga ggttttgaag
53641 gaagggggtc ttggctgcct cccactctta ggattgctgc tcctggaggc ttctgcaaYg
53701 gatggtgggt caaagccgga tgaggcctct ctccactcag cagcagggaa ggagtttttc
53761 ccagtcactc ccagcagagt acaaatgaaa gccttctggg tggagcctcc ccagtcctgt
53821 aaacagctca gttcagggac tggtRtacaa gctggccacc catctcagcc tctcatccag
53881 ctgaggctct ggccacaccg tgcaagtggc ttctagtttY ttggcaatMt gagatcagct
53941 ggctctgcaa gatgaaggtg gagccaaatg acacaatctg gtctcactga ggcccctcac
54001 ggtcattttt tggagactct aaataaacaa aaattttgag gacttcatga ttatgtggta
54061 gagcgagttt caaagtctct tatggaatgc agtgcaggaa cagtgaaaat aacagctagg
54121 gttactgggt gctaccaagt gccagggcta agtaccttaa gtacgctggt catgtaatct
54181 tcacccagcc ctatgaagaa ggcaggttat tatcccattg taaaagaggg aactgggaca
54241 ctaagaggtt ttaaatgact tgctcatagt tccaggtcca ggaaatagtg gggctaggat
54301 ttgaacccaa tctcaaatcc tggagcagtt ctggggaggg agcccctccc attataccaa
54361 tctctaccac tcatcaaagc agcctccaag gggctccact gaacttgtag ggttcttcag
54421 ggaaaaacag aaaatgactg agcagcccaa tcccttcttc caatcaccaa atcctccaaa
54481 ctgcaacccc tccaaggtgt ccgtcccatg gcctctactc acacacctgc aaagggacac
54541 tcgccacctc ccagagctgg ctccatttct gcacaactgt gtcccttaga aagaaagttc
54601 ctcttcatgt tgcgatggac ttggcctccc tgtcattccc acccactagt tataattctg
54661 ttcccagaag tcccacaggc gaggtggtgc cttgtgcccc atgagagctc tacagggagc
54721 aatggcagtc gctaaggtcc caatcttttc acgagtggac acagctccag gctcttcagc
54781 ctttcatggt ggacaccatg gtcaggggag cttttcaaac agaaatatgg tcatgccact
54841 ctgctttaaa aatgtccagt gactacacat tatgctgagg ataagaccca ggcctcccac
54901 catgctccgc aggcctgcac ggtcaggacc cggctacgtc ctgaaccctg cgttgcccct
54961 ctctttccct accttgttct tggctccagc cacgctggtc ttccctccga tcctcaaatc
55021 agctccccta cttcccctcc tcccaccacg gcctctgcat ttgtcatgtt ctctgcaaaa
55081 gcatcctcca cccctacctc tccacctagc tagtgtcact cattctgaga gtctcaactc
55141 aaatgctgcc cccccggtct aggtcggctt cctctgctat ctgctcccac agaaaccggc
55201 tcttcttcct ttagggcact tgagtgcagc ctgtttgtgt ggtgtttgtt tattcatttc
55261 tgcctccctc attagacatt acgctgtata agggcaagga ctgtatctgc ttctgttctc
55321 taggcatcca tagcacctag cacaatgcct ggcattagat aatgcactag aatgaatggt
55381 ttcagccccc ctcactctgt ggttgccatt actctccatt caacactgtt ttggaaagct
55441 gggcctctcc tccaccaaga gcagaaacta acRacatcac ccccaaactc aggcaatgct
55501 ggcagataag atattatctc ccccagggag gaccaagact cagtattagg ggctcacctg
55561 gttctgtagg cctggctgtg ggagggtcca tcacttggga gctctgattt ggatttcgaa
55621 cccctccaca gggtagacgc caggtgtatt ccggctgcga caaaactggt gcttaataat
55681 caataatcaY acccaaaaat tacaatagca cctactgtgc caggacaata ctaaatctgt
55741 attagtcaag tataattgaa tctttataac aacccaggct ggcactccca tttcacaggt
55801 aaggaatctg aggctcagag gggataactt gctgaaggtc atctagcaaR gagaatggct
55861 aagcttggat ttgaatccag gtctttgtaa atccagagcc aatctcttaa gcgccatact
55921 atatactctg gtgtttaaag atgtgtacca agaagggtca tatgggagaa gctgaaaaag
55981 aggccagcca ggaggtttct gtaatggtcc aggggagagc tgggatgcct aaggtggggc
56041 tgtggcagag gactggggaa aatgggacaa gtgacagcag atctttggaa gaagaatgat
56101 aagaagggaa aagaggccag gcgcagtggc tcacgcctgt aatcctagca ctttgggagg
56161 ccaaggcggg tggatcacct gaggtcagga gttcaagacc agcctggcca atatggagaa
56221 accccgtctc taccaaaaat acaaaaaatt accgggcgtg gtggtgcatg cctgtaatcc
56281 cagctacttg ggaggctaag gcaggagaat agcttgaacc tgggaggcag aggttgcaat
56341 gagccgagat cgtatcactg cactccagca tgggcaaaaa agagtgaaat tccatctcaa
56401 aaaaaaaaaa aaaaaaggaa aagaaaataa aaaaagagag gagagaagaa gaggagggga
56461 ggggagagga gcggagggga ggggagggga gaggaaagga aaggaaagag agagaaacat
56521 ggtgaggctc tggaacctag ttacacgtgg aagttataag aaaaagcaaa cccaaactct
56581 gatacccatg gcctggggcc tggagggagt cctccttccc accctgcact cttactcagc
56641 cttctccgtc ctagaccagt gaccctgaac agctgtgggg cagccctgcg ggactgtggc
56701 cagatccctg cccctgggta gcagcccaga gccaggccct ggacacccac ctgccaccac
56761 Rgtgctgttt agttcagaag gatgcagttt ctgaacccct cacttaacct tcctctctgg
56821 aaggtgtctg gggcagcctS tgtgtgtgcc ctggagcaag ggagggacac agactcacca
56881 gatggaagag gcgcgagttg gggagtgggg gcgggtgctc catggccatg gggggagggg
56941 ggtagcggat ctgggaccag tcctcgaagc catggtgtgg caccatggct ggcatgggcR
57001 ggtaggcgta ggggtaggcc ccgcagagat gttctgggtg gggctccaca tggtaacgct
57061 ctcctgaggc ctgcaagaaa ggctgacgtc tggctttggc ctgcaacagg ctgcaMagga
57121 cacccatctc atcaccctct aaatagaatg ttagctctct gaaggctagc aggctgggta
57181 ccaaaggctg gtatgtggtc atgaaaggct gctacatggg tacagaaatc tggatacatg
57241 ggcatagaag gctggatata tgagcatgga aggctgcatg ggcatggaag gctgggtaca
57301 catgcatgga aggttgtgac atgggcacag aaggctggta catgggcacg gaaggctggt
57361 acatgggcat ggaaggctag tacatgggca cggaaggctg gtacatgggc acggaaggct
57421 ggtacatggg cacagaaggc tagtacatgg gcatggaaag ctggtacatg ggcatggaag
57481 gctggtacat ggacacggaa ggctggtaca tgggcacgga aagctagtac atgggcacag
57541 aaggctgcta catgggcagt gaaggctggt acatgggcac agaaggctag gtacatgggc
57601 agtgaaggct ggtacataga cacggaaggc tggtacatgg gcacggaagg ctagaacatg
57661 ggcacagaag gctggtacat gggcacggaa ggctgggtac atgggcacgg aaggctgggc
57721 acatgggcat ggaaggctgg gcacatgggc acagaaggct catacatggg cacggaaggc
57781 tgggtaaatg gacacagaag gctgggcaca tgggcacgga aggctgatac atggaaacgg
57841 aaggctgagc acatgggcat ggaaggctgg tacatggaca cggaaggctg gtacatgggc
57901 atggaaggct gggcatgggc acaaaaggct gggcatgggc acaaaaggct ggtacatggg
57961 cacggagggc tggaacatgg gcatagaaag ttggatacat gggcacggaa ggctcataca
58021 cggccaaaga aggctagtca catgggcacg gaaggttgct acctggacac agaatgtcaa
58081 cagtgatgac agctaataca ttgtgggcac tgcctctgta ccaatactgc tctaaggctt
58141 ttcctgtatt actcatcagt tagttagcag aggatgtccc cacgcccgag cacacactgt
58201 gcatccatca ggctagctcc atgaggaagg taagaggatg tcagtgctgc tacctccctc
58261 aagcctcctc acctttgctt tcctcttctg ctgtctgagg gcttctcttg ccttctcctc
58321 atctttgaat gcttttagct gagagaagaa gggagggaga aaagactgtc ggcacaggtg
58381 gtttctggcc aggcctggat ccagggccta accagccaMR aggactgacc ggctgatgct
58441 tccctctggg ccaccatgcg tgggccagcc ccgggaaagg gccctgaggg cggagcacag
58501 cccaggctgt tgctgcaggc cacctgcttg tcacagtggt ctgtgcacag gtcaagaggg
58561 ttcacgtgaa cctcgctgct tctctgctaa catcttttag ttccactctg ggagagtaaa
58621 gcccagaact gactgtggaa agtcagaggt agagatatct tagagtaaaa ctcgtaattg
58681 tacaaagaaa gagtcagaga ggggaagggc cttgccgagc tcaaatatga ttgcagagct
58741 ggagagaagc agtgagggtc ctgacatcca gcctcaccag ggccctccaa tccatgcccc
58801 ctctcccSac gaggacaggc cagttgccct tcctgggtcc agtcactctt acctgggcat
58861 ttgacagggt gacctgggcc tgcagcttct ccacttgctt cttcaRctct tccttctcct
58921 cattcatgcg ctcacgatca ctgcgctccc tctggaagtc ctcctcgaag atcttcacct
58981 ggtgtggagg gagggtgaag agagggaggg ggatggtcct gagtggggat ttctcctcag
59041 gcccagaYtc ccatgcctcc tgctggggtc acagatggca gagctggggg tcgccatggg
59101 gagtgggaca gctacacctc cagcctccac cttaacttca aacagagtag ttctgtgtcc
59161 ctctatctta cattctgggc ttcaagStag gactttattt gaacaaagga tatatggttt
59221 taaaaaaacg ttgaaagcca tcaaccagtg caatcctttc actccgctga gacccacagg
59281 ggcagtgatc acagaaccag agtgtggtgt agaccagagt ttggatctgc tggttctgag
59341 ggcagagacc ctacaaactc Ygctagggct ccagggtgcc ctggcagagg tgggttgggt
59401 ggggcctgcc tctgtctctt agcctgtagt ttcacatcct tagtgcagat cagctgagac
59461 actggctagg ttcaaggcag tttaggagga ttaaatagca cagagacaat gggagagcca
59521 caaccttttc gctttctttc accccaatcc ttctgtgtcc ctcagggcta gtaggtcttt
59581 aataccattt tagtacctgc caatctcctt taaataaagg agaaaagaac tccagttcag
59641 agtcttcatt tggcaagagt atccggctag agtttaataa cattgttctg tttttattgt
59701 ttattgcgta cagttacttt ctacttgtga caaataataa atggtttttt ccaattacag
59761 cagtgatata aagctccctt ttaaattcat ttatttagaa tttatttttt aaatactagg
59821 taaataatag cacgtgtggg gcatagagat ggctaaatca taaaggtggt agaggaacga
59881 cttaagctag ggttatggtg ctggaaagga taaacctaga gccagtgggg ggccctggcc
59941 aatggccaga aagtggatta ctaattacag gttccatgtg attccaagcc gtctgggccc
60001 tcaggaaagc tccagctccc acagagcacc tcccacctga tttcctcccg gaagcctcac
60061 ctgctgtttc agcaactcat tctgcgtgac cagctcctgt ttccttagga gggcccctgc
60121 tccttctggg ctcccaaatg ctgttggtgg agatgatggc ggggtttgga tgctcagtgc
60181 ttcctccagg gcctggaatc aggaaaagat gggaccatca gcaggaaccc ctggaaatca
60241 gggccctttg gaggggtcat cctcagggaa ctccttccta ataataatca tactaataat
60301 agccaccact accagtccta tttaataaat gctgccctat ttatttgcat tctacacata
60361 tcatttcatt ggatcttctg cacagtgcca tgaagaaaag actatgatca tcatcctcat
60421 tttacagatg tgaaaactga ggctcagaga cctgaagggg cttgtccaag gtcaaggtca
60481 cacagctagt aagtggcaga gcacagattc aagcccagca catgctccta cccagcaggc
60541 taaactgggg ttgtcatgca gaaggggaaa cctccccttc cctgaacatg tgtggctcaa
60601 ttcttagcac ccagacctag aaggaagatt aagtcctccg ggggaagctt cagtctcccc
60661 aacaactgcc ctgccctgag attcttcact ttcagcaacc cattcccatc caaaccaccc
60721 cttccatcct caacacttat gtacatagag aagactggat ttaatttctg cttagttaat
60781 attttccctt tctaacactg aaaatgggct gaaatatcat caaataacat tttgaaaata
60841 aaaaaatact cttaatctat catgttcaca atagattata taaacagtgt gcccacatct
60901 tcttaaaggt ttcagttaac cgtgtttccc ctttaactct gttaacatgt tactcagaac
60961 tctggaacat gtctcaagac tgggcaccct ggactttggc cccaggacag catcgcccat
61021 gggcctccct ggaccgaggg gagatagaga cctggcccaa cttggactcg ggcttccacc
61081 tggtctcctc agctccccta gacacgcaaa gaaactgcag gaccgagagt aaattcagga
61141 tgctccgagg ggaagttatg gctccacttt atcttcctac tccaccccac ttttaaatga
61201 aaagactgag gtccagcRac agggagatgc ctaggtcatg agcaaagaag gccacatgcc
61261 ctgcattact catccacatg gtgtctgtcc ctggctctgc caagaaaacc tccaaggtca
61321 tctttatgat cagcaacatg gacaattctc tgcctggatt tagagcccca agtcccccag
61381 ctcaggacag atcgcgtttc tttctctgga aacaggtcaa gggccccaga cttggcataa
61441 tcagtgttta caaaacacca aaaccaaaaa gaaagggttt ccccagcagg cctaggaatg
61501 ctcaactcat gccagagtcc actgtcacca gggtcaggag gcagagggaa aggaaaaaca
61561 attagcacga aacgaaaagc tgagccatga acttaactcc tccaggccct cagagaatgt
61621 ttcctttcaa agctccctcc tgtcagcttg catctttgaa atgtgctgga aagcaggcag
61681 aactagagcc gttcccaccN gggctcggat ggcaaaactg aactggagag gctggagagg
61741 ctaagctggg actagaccac tccgcttggc teagaccaga gggtttctgg tttcctcagc
61801 ctctaccctg ggggccccat ctccctggat cccactgcac aagggcgggc ccctcagccc
61861 gtgctcctcc actcccatca gtgtttgctg aacatgtgct tgatgccagg cgctgtacaa
61921 ggcgcccagg gtatacacct tggtgaaagt atgcacctcc caccaccaga tgggaagaca
61981 gaattaaata agtgatgacg agccatgaga aggagtgtca gagaagttgc tggtacctca
62041 ggagcataga ccagggcaac ctgaatgaga ttctgggaag gctttgctga gcaagtgagg
62101 gtaaaacaaa ccctgacaag cagggtgggc aggtgaggca caaggggaag agtgcacctc
62161 cagagggaag ggtgacacag gctaattcaa gggctgagtc actcccagtg tgggaggcaa
62221 tggcagagca ggcgggaaac aggatgccag ctcactgact gggggttacc cttcctaagc
62281 tgaatggttR ggggtgccag tgatggggtg ggcattgcag ctggactcaa gggagcccag
62341 ccaagggacc cgggccaagg cacccacctt gttgagccgc tggatctcct tttcctggta
62401 ctcacgctgt cgggtgagtg ggctcagctg atcctgcagg tacttgacct tttggcgcag
62461 ctccttggcc tctgctgtca gctgctcctt gtcggtctgc agggaataca aggttggtaa
62521 gctggctagg atggcctctc caggaggcct cggattctct gtcctgcctg gcccagccct
62581 tacgttctcc cctggYcatc acttcacaat gctcacatgc aaagcacctg gggtcggtac
62641 agtaatgtcc atccggtcct gccctcaatt attatgtgtc aatttaaaat aataattaaa
62701 aaaagaaaaa gcagtagctc agttgagtaa aagaccctgg attccgacac cagttcatcc
62761 actaagcagc tgagacccct ggcactgcca cagccctctc ttgtctaagc ttacctacct
62821 gtcacaggta cggggttcct aacgtgagtc acacagagac ctcaggaggc ctgtgagccc
62881 caaaactgaa tgcgaaattt tgttgtgtgt aatctgctag aaagaaaagc catagttctg
62941 accagattca catgggggtc ttccgtgatc cacagaaagc gataaactac ggataatccc
63001 taacagacac tgcagctcat acagccttgg agcctagagg agagatgaca cgcccagaga
63061 gaactgtgac ataaggcaga gagtgctcct ccagaaagaa acaaaatact gtggggctaa
63121 aagaaagatc accacagctg agggaaggct tccaggaggc ggttccatac cctctacgga
63181 accagcaggg cttcgccaca gccagccaag actgggatgg cctttagaca gcatcaagcc
63241 ccagccctgt aatacgatgg gaaacaaatg tccagagagg ggaagtgacg tacccaaggc
63301 cacacagtga gctggggcta gaacccaggt ttcctattcc cttgctctct ctccttccat
63361 gtcaaaggag gtgttgaaac taggggatgg cagcacagtg cagtggaaag accaggcaca
63421 tccccaaata aactatatat tcctatgtgt acatatatgc gagaaaggtc tggaaacaga
63481 tgcaccaaaa tggtaacagt ggttccctct agggagggcc tgggatcatg gcagtgggag
63541 gtagtccagg gaaactttca ctttgtagca tgtggacatt tcataactgt gtattcacag
63601 catgtgatta agagaccagt cgcaggggtt agttttcacc cagccctgtg tgacctttag
63661 tcagtacgga ctgcagaggg caggcttggt cagacctccc tgggaattgt tctggactcc
63721 ccatcaagag gctcccggat gggcatgggc tttaccgaaa ttcatcctta tctacagggc
63781 cacgccttgt catgaaacgt cctaccttcc ctgtagctga cagggaagag ccctcacatt
63841 ccagggattt gtctcaccta ccactgtgag caagggagag gggccaaaga gccaggatgt
63901 ctggctgcga gtcaccaggt atgactgtgc actcataagg aaagacagaa gtgttctccc
63961 taccacacct ccaaggtaag ggggtgtcac agacgtaggc tgactgctca aataaggccc
64021 ctggtgtccc atgctgacgg gaggaactSt gaaaggcaat ccaggttgaa aatgtccatt
64081 tttaagctag gcaaaagacc atgcccagtt cccatgaagg ggaaaacatg gtgacataaa
64141 taaaatctcc caagagtctg gcagtcaagt ggcctttcct ctttaagctg ctccaaggtc
64201 ctgatgggcc ttggctggtc tcattcatac atatgactgg gcagatacta ctacttcagg
64261 ggacctgttg tttcccccca agaaagaatc tggtctcact tatgcccact ctctgatctc
64321 tgtaacttct tcttcgtagt actttttttt tttttttttg agacagggtc tcattctgtt
64381 gcccaagctg gagtgtagtg acacaatctc agctcactgc aacctccgcc tcccgggttc
64441 aagcgattct cgtgcctcag tctaccgagt agctgggact acagacatgc gccaccactc
64501 ccagttaata tttgtatttt tattagagac ggggtttcac catgttggcc aggctggtct
64561 caagctcctg atctcaagtg atccgcccac cttggcctcc caaaatgctg tgtttacagg
64621 catgagccac catgcctggc catacatttt tcttttagag aaacagggtt tcactctgtt
64681 gtccagtctg gagtgcagtg gcacaatcac agctcactga agcttccaac tcctgggctc
64741 aagcaatcct cccacattag cctgagtagc taggtctaca ggtgcacact gccacaccca
64801 gtgaattttt taatttttgt agagacaagg tgtggctatg tcactgaggt tggtctcaaa
64861 ctcctggcct caagcaatcc tcccacctcg gcatcccaaa gtgccaggat cataggcaca
64921 agccacccaa cccggccttc tctgcaacac ttaacacact tttactttat ttgcttctgt
64981 aattagttgt ctggcacctc tttcccagcg agggcagggt tgagctgttc ctgtggactc
65041 tatgccaaag cacaatgcct gagacaggct ctgaagacaa atgccgggat gaatggataa
65101 atgaagctct gtctcatgtt gtagcccctc cccaacaaat caccatcact atgctgaaca
65161 cttggcagca tcccacccca ccaagtgcca tacagcaggc ctgcttggat cttttttttt
65221 ttttttttta gcaagagttt cactcttgtt ccccaggctg gagtgcgatg gtgcaatctt
65281 gactcactgc aacctccgcc tcccaggttc aagcaattct cctgcctcag ctacctgagt
65341 agctggaatt acaggtgcct gctgccacac ctggctaagt tttgtatttt tagtagagat
65401 gggatttcat catgttggcc acgctgatct tgaactcctg acctcaggta atccacccgt
65461 ctcggcctcc ccagttgcta ggattgtgag ccatcgtgcc cggccacctg cttagatctt
65521 ttatatggtg tgcttccctg tacagttccc cattccctac aattttaatg gaacaaaaag
65581 ttctgaataa aaacaagaaa atgtgaaaag ctgtagttaa tgtaactttc tcaattttca
65641 gatgggaaac tgcagtgcag ggaaggaacg tgcctgtgtt tttggtcacc tagctctttc
65701 gcactagacc cccgggtctc ctgactcctg cccctggctt gtttgctcca cagaactctc
65761 cgctaatgtg gaggggaact gactctgcag ggaccagcag tcacacttgc ctcctccatt
65821 tcaatcttgg acttggccag gaggagcttg cggtcaaagt cacgctgctt ctgctcccgc
65881 tcggcctcca ggtcagtcac ctgcttctgc aaatcagcca gcttctgacg cagctcagtg
65941 atctgggttc agaggcagag aggagtgtgt gaggcaagga tgtagagcac ttgcaggatg
66001 tgggcagggc ctggctgccc ctgggccctc caacactgcc cccaacttct cctctgggcc
66061 atactggggc tcagcgKgKc cactcYccaa ggttcaaagc tgctaaagag gcagMgagat
66121 gaagaccagg catgccctgt gggcgtggcS aggaacccca cattaccttc tgctcatact
66181 gctgcttcat ggaccggaaa tgctggtccc actgcttgtt cacttccagc agctgtgggg
66241 agagactgga gttagcagga gatgagcaga gaaggaacaa ggagagccat ctcctgcccc
66301 atgtacacca gcgtcccaag gtgtgctcgg tgtgtgtgaa tagtggctac agacactgtc
66361 tccatccctc agctctcgaa aactcaatgt tgctcaaagc catccacctt catccactgc
66421 tgcattttac cttgccagga aagcaagatt aacactaaga catggaaact acatMgtttt
66481 ggtgaataat tatggaacac acacacctct ttggagggca gtctgggatt atgtattgaa
66541 atttcacatt ttcatcaact ttaaaccaat aattccactc tgaagaattt aaccaataga
66601 aacacttaca caagtgtgca aatatatata cacaaggcta atcatctcag caatacctgt
66661 attacaaaaa aatccagagg ccaatctaag cacagaagat aggttaaaga aacgatagta
66721 taaacagaca atgtgcagct gttaagaaaa taagaaaata ggccgccagg cacagtggct
66781 catgcctata attccagcac tctgggaggc caaggtgggc caattgcttg agttcaggag
66841 tttgagacca gcctggagaa tatggcaaaa ccccatctct acaaaaaata caaaatttag
66901 ccgggcatgg tggcacgcat ctatagtctc aactactcag gaggctgaga tgggaggatc
66961 acctgaactt aggaggtgga ggctgcagta agccgagatc atggcactgc actctagcct
67021 gggcaaccaa agtgagaccc tatcttaaaa aaaaaaaaga aaagaaaaaa agaaaacaga
67081 aaataaagca cctaacacac ttcctcagtt tgaagatgac actatccacc atactcttga
67141 tataacaatt cctcagggaa gaaaaacaac cctacatcat caactaaata aaacaaaagg
67201 agtattggcc ctgcccctta agatatgtca taaggatcca caccaatttc aaaaacattg
67261 catatgataa aaaaaattac aaagaattaa aggaatatag catatgttat agatatgaat
67321 agatactctc aaaggcagtt aatttttttt ttggaggcag gcctaggctg gagtgcagtg
67381 gtatgatcat ggctcactgc agcctcctga gctcaagtga tcctcccacc tcagctcccc
67441 caagtagccg agactacagg tgtgtactac cacacccagc taatttattt tttgtagaga
67501 cagggtcttg ctatgttgcc caggctggtc tcaaactcct ggttgcaagt aatcttcctg
67561 catcagcctc ccaaagtgct gggattacag gtgtgagcca ccacgcctga cccagttaat
67621 tttttaaaaa agaaaagaaa aaataatctg taaagcagtg tgtgtatata catatatata
67681 cacacacgta tatgtataat tttattctac tttgaaaaaa actgagggat attcaacaaa
67741 ctgtaatcag ttgtggtttt ctctaggagg tggtattctt gatctttttg taatgagcat
67801 tatatcactt ttgaaatcag aaaaaaagac gcttataaca atacaaaaag aaatctaaaa
67861 ctaatatgca catcaagaag ctttcaaatg tgcgggagga caaggggagg caggctcagg
67921 gttctagaga agcacctggg ctgtgtggag aggggctaga cggaacagac agaacaagtg
67981 tctaccaaaa aggagctgca cttcgacctc gggtctggaa agactggact agactggggg
68041 tgtgaggagg gggaggaaca catgtgagat ccatgggaag aggcggaatg gactgcagca
68101 cagggactct gctgcgtggc ttctctcttg gcagcagagg gaaactaaga gcctcttacg
68161 aactggctcc tgcaccttag tgggactggg ggacatttaa actacctaaa gacctggcca
68221 tcgccttaat ggagtttagg aatccactca gtattctgtg gcaggcttcg gctgctggct
68281 ggtccttttc attcttgcct aagaaaattc ttgaaatggg agtctcaaaa tatctgcaaa
68341 gtagctacac caatagaggt aggtagctat tttttttttt gagatggggt cttgctctgt
68401 tgcccaggct ggcacgcggt ggcacggtct cggatcactg caacctcagc ctcccgggtt
68461 caagtaattc tccctgcctc agtctgagtg ggtgggatta caggcatgtg ccaccatgcc
68521 cgctaatttt ttgtattttt agtaggcaca gggtttcgcc atgttggcca ggttggtccc
68581 gaacctctga cctcaggtga tccacccgcc tcagcctccc aaagtgctgg gattacaggc
68641 atgagccacc gcacccagcc aaggtagctt ttaacgttaa aaattttgac agtaaattta
68701 tggagcctcc aggctagtgg gaagcggcct taatcccttc cttccatgat gggtcctccc
68761 taaagcatct ctcaagggtc cctccactcc tcatcggtga tgctaaaact tgcctaactt
68821 atcacaacct ctttccacca tccaaggtct accataggag cctgcctaaa ctgtagcccc
68881 aacaatttta ggccatgccc cacatgccac ctcctcagag tgaccactgt aggcaaaggg
68941 actgatcaag agaggacagt ccagggcaag gagtcagacc tcatccatgg gcaggcaggt
69001 cagtggtcac catgccaact cctcatggtg acctgggacc tgccagagcc aggaggocag
69061 ggcaagcctt tgtgctgctg gtcccgggag gtaagaggga tcctcccact actgccacct
69121 cggccaccta cctcactgcg ctgctgctcc agcatcttca ccttcttctc ggctgcRccc
69181 aaggccacca cctctgggac cttacctgcY gtcacactag cctggggaga agcacagagg
69241 agccttcacc aaaacctcaa tacaaattat ctcacccagt ctcggtgctc ctctaagacc
69301 cctcacccaa gKggccccag ccctctgtct ccctattcct cccacccagc aggacaccac
69361 taaccatccc agtcatgact acgtcccctt ctagaagtct acaagatgat gattccccct
69421 ccctgactca gggtggctgc caggccctct ccccttccct tctcccctcc ctacggtcat
69481 tctccctgtt taggtacaac ctctaaggta ctaccagcca ctctcctcac tccctacaca
69541 catcagccaa aaaactctct ggcctggaag gtaagacatt tgagatgtag atacagacag
69601 atatagatat agatatactc attctataaa acatatatgt tttataaaac ttttacaaaa
69661 gttgaagtta tataaaatgt ttatttaaaa acatttttag aaagatgcag aaataaagat
69721 ctgtgtaata cacatagatg tttcccagct ctgtcctctg taaggggcta aagcagagac
69781 atccagcagc actgagcaaa cctagtgctc agttggtttc taaataccat tttcttttta
69841 ttaaaaagaa acagggctgc tttgaggggc tggagcagat aaaatataag gtgagtttga
69901 tgcatcttat agtgatggaa agtaaggaag tgctcaaatg aataatacta ataacaataa
69961 taataaaaga gcatgtcaaa agggcacaag agacaacctg aaaaaaacat ctatggccaa
70021 aacgggaaca ctttgaacag caaaatacat cacaatagta ttggattata agtcacagta
70081 tgaaataaat actcttgagt tcatactgat agaacaaaat agctgaatca ataaataaat
70141 gggggagaag ggacagctct tccttacaaa agaattctga ttaataagtg taggaagaat
70201 gaggaaaata tgaaatcacc attccattac tacagtaata actRctgcag gtggagatcc
70261 accaatggtc actaaaatca gtgagtgaaa gtttaagaaa aaatagagta tttgcatagt
70321 ctctaagttt ctcctcaaaa ataaacactt attaattaca aagggaaaat tggtcacttg
70381 attgcagaaa cttgaaagac agcacttggc caagtgatga aggttaacag tagtaaaaca
70441 ctgacatcat gtacccctgg tctgctggac tgagaaatat tctgcacact ctgatgtaca
70501 cagaacgtca cctccgtgag attcttgcca aaacacgtaa cctcaatctc ctcatgagat
70561 ccaatcactt gtgaggcctc aatcgtgaca gcaaataagt ctatactgaa aaacattcta
70621 caaataactg gccagtacaa ctcaaaagtt tcaaggtcat gaaaagcaag gaacagctag
70681 gaactgtcac agactagagg aaaatgaggg agaagagaca actgaatgca atgtgggatc
70741 ccagactcca tgctggcaca cgacaggaca tgagtgcaaa tctggtgaaa tctgaataaa
70801 gtctgtagtt tactaagtag tacatgccat taattcctag ttttgatcat ttaacctggg
70861 ttctataaga tgttgccgtt aSgggaagta aaggaagggg ataggggaac tctctagact
70921 atttttgcaa ctcttctgta agtctagtta taccaaaata aaaaggttta aaggttaaaa
70981 aaaaaaatag caagtgctgg ggtgaaacag cagacaaggc cagtagtccg gagggtgtgt
71041 gtcgactcta tctccaagcc cctctgtgat ggacaccaag accctcgtct tctctgggct
71101 tcaatgcgat gagaagctca aacagaagaa ggtctgcagg ccccatccgg acttctgaca
71161 ttgtctgcca tcatcctgga gtcagattct ctctattctc agcgttctct ttccagcgtt
71221 ctctttccct ccacactgca cccccaagtg gcttcaggta gggctgggtg cagagcttgc
71281 cccctacagg ctcaggcctg taagcacacc atagcaccaa ggagcaaggt gagggggagc
71341 cctccaagga aggtcacccc ctttgagaaa ggggacagac aggcctgccc acttgaaatc
71401 tgcggcatct tggatgggag ctgtgcttag caaaaaggtc aacaagttac cggggaagga
71461 ggaagagggt ggattccagg ctctagggtg gtgcaccctg aacctccgcg agggagagag
71521 gagtccacgt ggagtcagca gagctggctg ctggccccaa atctccccta aagagcaaca
71581 gctaacgttc actgtttacg acgctaaacc tactttacaa gcattgcatc actgaatcct
71641 cacaaccacc ccttgaggca ggtgttacta tcttcatttt acagaggaaa ctgaggccta
71701 ggagtcttat gtgacttacc caaggttgca gtgctggtaa aaggtggagc tagtattcca
71761 cctgcatgca attccaaggc acactcgtcg cccctctgta acacatgcct ctaatgagac
71821 aggtgaccct ggggcacaag ggcaagtcgc tcccactctc aggaagggca ggaagagcat
71881 ctggctcggg gcattcaagg aagaYgagtg gagattaaag ggcacaggaa aagtaaagag
71941 acctgtccag ggaattccac acctgaggca gctcagagaa cccaagggag aacagccgag
72001 tctacaagtc ccttccacaa gagggcagac aaggccggat ctgatttggg gaataaaggg
72061 aagaaaagag gatctccatg ggctgggatt cggaacacca gggaagagca gactttcacc
72121 atttatcagc atgtttccct ggccgtaccc tacatctgcc tgatagcagg tcacacagaa
72181 atcctgatta tatgatgtta cttgacctct ccaaacctat ggccatagct atagaatgga
72241 atactagtcc cagccttgcc tactctatag gtagagatga ggcccaaata agaWaataga
72301 tttgcatgtc ctgggtaaac tctacattgt cacacagtaa taaggaatta agccattacg
72361 gtaattgcta ctattgcata ggtgtagggt gggttaaaga cattgagccc tactgttcag
72421 ctattaaacc ccattaaagg gtgaagtaaa aacacgttgg taaagagttg ggactcagct
72481 ctgcagcaag gggaatggta acgactgggc aaggggtgca gagtctgctc ctgcttggga
72541 cctggatgtg gccagtgctg ggactagagg ataggggagt Rggccagccc tgcagcatca
72601 ccagaccaca cagacactag ccaacaccct cccctctcca ctttagaggc tccagagaca
72661 caatcccagg agcttagcag aatccaaggg agcttgctca accaacctaa ttctgccagg
72721 aatatcaaag atgttaaact gacttttggg aaccactctc taagacagac cttgacaaac
72781 tggaatgtNt ttacggaatg cacccagact gagcaaacga ctcaacacca ccttctatga
72841 gcaatggctg aaggaactgg agctatttag ccaggagaag agaaggctta tgaggcccat
72901 gaagccacag tgactaccag cagcctctaa gcatggaggt caggccaagY aaagaaaaaa
72961 acacgtaaca gctagaacct tctaccactg gcactggctg caggagggag gaggctcact
73021 gtcactggag gtggtaagca gaggctgagt acctgcaacc aaggatgcta cagaaggaat
73081 ttctgaccac atacctgctg ctgtcYagcc actgccttct tgcctgtccc ttccatcttc
73141 ggtgagcctg gccgcccaga cRcaccctct ttgttgccat tgctcatcaa caacttcttg
73201 agctccaaat tttcctccct gggatggagg taaacagaga aatcacaatg ctgaccctga
73261 gttaagaaac ctMcagggct ccttaatgct cactatcgca tgagttgcag ctgagcccca
73321 gggagtactg caggatcctg aaacctgccc tcctggaaaa ccaaaaaggg catcttcagc
73381 taccttgctc tcaatctgtg gaggtctctg attgtcaatg gcagggtttc cccagctgtg
73441 gaatttctat gcacacaaga ccttccggat atatccaaac actgcattaa ataaccctgg
73501 atcacattct agtgagaaag tgacaccctt tccaattatc ttcacatttc ttgtgtagac
73561 cacggagacg gtgcgagctt ggtgctaaca ggccctgggc acctctctag ttactcgcca
73621 gtttccctcg ttaacacaga aggtgcaggc tgcaggccca gagccttcaa tgtgcaatga
73681 tccttagcct gaatttatta acatcctttc actttcattg tatttagagt tatcttctat
73741 ttatcacagg taatgctgtt ttccatttac tctagttaaa gttgccttta taattttaag
73801 taaaaagatg aatcaaatta aagtaaaatt ctaagtaact aataaggcag gtgatagagg
73861 agatcataaa attagtgatg tggattcagg tctgactaaa atgtttcttc tttctttttt
73921 ttgagatagg gtctcgttct gtcacccagg ctggagtaca gtggcccaat ctcggctcac
73981 cacagcctcg actgctccta ctcaagtaat cctcccacct cagccacccc caacctcccc
74041 caccacacac ccccaccctc ttgagtagct gggactacag gcacaccacc acacccagct
74101 aatttttttg gtattttttg ttggggctgg Rggaggggtg gtgatgtatt tcaccaggct
74161 ggtctcaaac tcatggactc aaagcaatcc tcccgcctta acgtcccaaa gtgctgggat
74221 tacaggtgtg agccacaacg cccaaccacc acttctttat gttctatttg ccagctgagt
74281 ttccgcttga aaaaccaata agtaaaatga ctttcccaag gtcatacagc agagacttgc
74341 attcttgcct tctaatccgt gacttttttc cccagcttca gtttctgaat ccatggatgg
74401 ggtcaYtagg gctataaaaa ccccaaaagt gcatccttgg ccatccaaaa gcaaataaat
74461 tacacactga ttcaaaatag ccagtggctc agtagttaaa cattacttct gtggccatac
74521 ttacaaagtt tatattcctt ctctttctgt tgattctaat aagaaacagg agaggaggga
74581 aaaacagagt ggcaagtcct gtctgtgaca cgggcctttc ctatgtgcct gagcccagct
74641 ggtacccacc agatgactca tcacYcaaac tgtccaggac cacagtgcag gcaggaattg
74701 tggccactca caggatggag agcaaggagg cccaagttcY tgttgtaaga ctccaatcaa
74761 gtccctgcct ctccctgggc ctcagtttcc cctctgcaca gtgagaggtc tgtctgcatg
74821 atctccaagg gcttgtcaag ctctgacagt caagatcagt gtttcccaag gcgtgatcct
74881 gtgcctacct cgttcataat cacagagact cctggtgaaa tgcaggctcc taggtcttgc
74941 ctaagacctg tcaattcaga atcttgcaaa ggaaggaagg gaaggcgtat aaacttgtaa
75001 tgcctttccc tgaccctcaa ccctcagtta agtctaagcc ccctagtcca gtgggggagc
75061 tctcatgttc tcaggaagac agagcaggtt gggatggtga ccagcaggag gcagcaaagt
75121 cctgagtcct ctggacagcc agttcaccgt taacagggga gagaggcaag tgctggagga
75181 ggaaggaagt acaatctatg ctgaccttct tctgaagctc tcaaggcact ctatacacct
75241 cccaaggtgg caaaagggcc agagagaaaa taccaaaaga gccaaggaga aggatcaaga
75301 gacgtaacct ccaaataggg ctctgggcac agggcctcag agccacggtc ctcaacccag
75361 aaatcagtgc tgcacaccag cYcctcctcc tgcagccccM actcctcacc ccttctctga
75421 ggggcctgaa cttaccgcag cctctcggca gcctgatccY gctgttccag gcccttatcc
75481 aacttggcct tcagagcctc gttctccttc cgaagctgct cacacagggt ctgtggggca
75541 ggggaacaga cagggtgagg gaaaggaggg gcccctccca ggtgcaggct gagctagagg
75601 atggtggggg gcctgtagcc accccagggc ccaactcctt atcccctttg cctggcctca
75661 ccccatccct ctctaaggag ctaagccact tggccaccca cgtgtcctct cccttgggag
75721 ccaactacgg agaagcctga ccaagtatca gccatggggc cttctgctcc tccaagacag
75781 acagctccat cccttctcct tcatccctgc tcccactgcc gggaccccta ttccccacca
75841 gcctccccca gctgacttct acYcttcttt gaagtcttag tttaaatccc atctcctcag
75901 agaagtcctc cctgactccc cgatccagct acattaggct aactccgtta tccttgctca
75961 cagccccccg cttttacgct tctcagcacc tgtggcagat aaagctaaag aactgttcgt
76021 gctgctgttt tcttaatatc taccccctta cccctaatta attcctgagc tcccggaggg
76081 ctgggacccc taaccatYct cttgttcact gccagatccc cagcccccag ctcaaagttt
76141 gtggaaggaa caacatgact gaacagccaa atgagcaaat ggacaaatga gcaggcgagt
76201 cctagggggg ctgaaaggca caccaatttg caggtgtgag aggtgcccag tgttgccaag
76261 gggaaaacca gaagacaagg cattcagtcg ggacctcact atgccctcac gccctcacaa
76321 tgtggctgtg acacttggtt cctcctctac aaaagaaaga caactgtttc tctttttttt
76381 tttttttttt ttttttttaa gacagagtct cactctgttg cccaggctgg actgcagtag
76441 catgatctca gctcactgca acctccacct cccaggttca agagattctc ctgcctcagc
76501 ctcctgagta gctggaacta caggtgtgtg ccaccatgcc tggctaattt ttgtattttc
76561 agtacagacg gggtttcacc atgttggcca ggctggtatc aaactcctga cctcaagtga
76621 tccacctgcc tcagtctccc aaagtgctgg gattacaggc atgagccacc acacctagcc
76661 cagggacaac tgtttcttct ctgcctcctc tatagggctg ctgagaggga cagatgagac
76741 agtgagagtg gaagtgtcca tgaaccatca gtcacttgct tatgtgcggg gactcatatt
76001 ggaagctctg tgaactgggg gcatggaaga ggggtcgcca gactcaggac ctcacaggct
76861 ggggggaagg gaaggtgctg actagcaatg ctagcataca gtgtcagcac agagttgttc
76921 aatctttcca cagatcctca ctgagcacct cacctgtgcc aggtacattg ctcggtgttt
76981 ctccatatac tgcctcactg gtgcctcaca acttcctcaa agctaagtaa ctcatctcta
77041 tgttaaaggt agggaaacca ggtaaaatga tacagtctaa caggtgcaca gctgctaaga
77101 ctcatcactg gcggggagta aaactccaat tggcagaccc cacactggtg tctctttccc
77161 ctgtgatgaa agatcagaga agggagagag atggtgccca aaggagggga cagcctttga
77221 gctgggccag gaccagggcc attcactaaa cagccatctg ctgccttcca cctcccaggc
77281 ctgttgcagg tggatgcaac catgtgacta gttctggcca acaaaagagg tgtagaaggg
77341 acaagcatca ctcctggact ggcctacgca caattctcca gtctcttttt cctggtagca
77401 ggggctgatg tggatgcctc gtattctaaa gggcaccgga aagttgggcc cctgtctccc
77461 tgggactcta aggaactgtg tagagcagaa acaaacccct gccacacaga ataggaggga
77521 aaacttttgt tgtgtaaccc agggagattt cagagttgtt tcatttctga ctcacaaagg
77581 agactcttgg gaaggctgaa tggaggacac agccttcaac tggaggtggc tggacaatga
77641 gggagatgag gcttagcaaa cagagaacca gctgggcatg gtggctcaca ccagtaatcc
77701 cagcactttg ggaggctgag gcaggaggat cacttgagcc caggagttca acaccagtct
77761 gggcaacatg gcaaaaccct gtctctacta aaaataccaa aaaattggcc aggcataagg
77821 gcgcatgcat gtagtctcag ctattcaggg aactgaggtg ggagaactga gcccaggagg
77881 tcaaggctgt agtgagctgt ggtcgcacca ctgcactcca gcctgggtgg cggagcaaga
77941 ccctgtcccc caacacccct taaaaaaaag aaatcaaaaa attattgtta tgagtccatt
78001 caatgcctag cagagagcct tatgctatac ctgaccttga atatgtttgt ttagtgaacc
78061 aagaaacaca tcagtgaatg aatgaggagg aactggatca atacacctcc acccaggcag
78121 taaaaatacc caagatgcca tgtctctggg cgaggcctcc agggaacaga atggcctgct
78181 cctagtccct aagggaaggc caggacaagg cctcacttgc tgtcctaccc ttgcagtcta
78241 gagagctgtt tccttggagt ctctgggcca atgccctggg tgcaaaccgg ccaatggata
78301 gaatcatcag aggtcacaaa gatcatcacc ttccatccaa ggtgctacga gagctatgag
78361 ttcatttgat catttattat tttgtccttt cattcattca ccaagtgtgt atgaagtgcc
78421 acgaactggg aYgcaacagt gaatacaaca gacatggtgc ctgcccccct caagcttacc
78481 ttctagcaga gagtataaga aaaaaaagag tataagaaaa aatgtattaa ataaaattat
78541 tacaaactat gataagagct aagaaggaaa taaacaagga ggggactgaa ataaaaaata
78601 acaKaggttg gcaggacccc ttttaagggg cctaggatct atccccatct caaagctgaa
78661 aagtggatgc gcaaagtgct ctcaccccag gcaccttcct accctgccca cagcctaggc
78721 actgtgccag gctcaccttg ccagggttca gcctggatac cttgaagcct cactgacatc
78781 ttaaaggggt tcccttctct cagcctggct ctccaggcct ctgcctctcg ttcagcctca
78841 cctcccaaca cacccctccc ctccaggtgg gatgggcatc ctccgtgctc ctctaccccc
78901 acacctctcc tcatactgca tctccaacct gattgccctt catgccctcg gcaaccaagg
78961 tcacgcaagg gggactccca cttctgccat cagcggggca cttgagtgcc tgctgacatg
79021 aatgagacca cagatcagag aatggcctgg gaggagagtc tgcagggagt aaggaggctt
79081 caagtcatgg ctctgtcact aaggtggcgg gcactcagat cacgagcagt ccctcagaag
79141 cctccgtttt gccatcttta gaatggtcac caattataac tgaacacttg cccgctgcta
79201 aagcccacaa ggctaagacc ccctgcccag tgtggatgcc cgtcatgaac tccgaatggg
79261 gacctctctt ccagcatgga ctaggttctg gactgcactg ggtgcttcat gtacatggcc
79321 tcatttaatg cagcaatcta accatggagg cacttctagc tcatgttact gataggaaca
79381 ttgggggata gagaggataa gtgacttgtc caaggtcata cagcaataaa gggtcactac
79441 tggaaccaag gaccatataa ctccaaggtc tggcctggcc tagcaaggaa atctgtccat
79501 tccccacagc acagggaggc tttcaaaagg aattcaatag gggaggcagg ttgggatggt
79561 ggtcaagagt atgggctttg gaatgcgaga gatcgaggtt ccaatcccta cttcaccatt
79621 taccagctgg gtgaccttgg gcaaaaaaag ggctgcttcc ccacctgtga agtggtgata
79681 atgagggtgc ctaccctggg gggaaagagg cattatgaaa ggagttgacc tttgttccct
79741 cccttcccat cccccaaccc tgcacatgag aaataaaggc agggctgctg ggggtatccc
79801 caaccatgac actcgcattg cccatcccgt atttccccat ttccttcctc ttcctcatcc
79861 ctccccacaN cttccttatc ctgttcatca ccctgaacaa aaagcccgaa gtctgcccac
79921 aacacagSct tcagaagtcc ccatggtacc agtggattcc caggagcagg aaggtgggaa
79981 gagctcgggg ggaagcacgc ctgcctgtct cgggctgccc tccccacgcg cctcacctgc
80041 agaatggagg tgcgctgctc attcttgtgc accttggatg ccagtcggtt gaactccagg
80101 gccatgcggc ccaggtgggt gaagagctgg ccgtggtccg gctcctcggc acacacactc
80161 agcgtggtct ccaggcgctg caggtgcagc atcaggttgc cgtcctcacg ggScaggggg
80221 cccagcRcct ggagagggaa agggcacacg gcccactctt caccaaggcc tgagtaggcg
80281 ccgccagtcc attctccctc tgcttcctca tttctcatga ctcagtttcc ccctgtgacc
80341 ccagctcagc cccgggaaat tctccagtta tgcaggttcc ctgtctgggg ctgactcaca
80401 gggcagaacc caggactcac aggccactgg tggctccaca gcagcggggc caccatcctc
80461 ccacacactg ggccttgcag agtgcctgac agtaagtcac ttgtctgctg gacctcagca
80521 tcctcatcta ccaaatgtcc gcacaaccct tccctccctt caacctcaca gggttcttag
80581 gaggatgaat tgacccctga cagtaacaag cccagaaaag ggacatcact gcacagtcag
80641 ccctccatat ccatgggttc cgcatccatg gattcaacca actgcagatc aaaaatcttc
80701 taaaaaaaca aactggccgg gcgcgatggc tcacgcctgt aatcccacca ctttgggagg
80761 ctgagcgggc agatcacaag gtcaggagat caagacggcc tgagtggtga aaccctgtct
80821 ctactaaaaa tacaaaaatt agctgggcat ggtggcacgt gcctgtaatc ccagctactt
80881 gggaggctga ggcaggagaa ctatttgaac cagggagtcg gaggttgcag tgagcagaga
80941 tcgcaccaca gcactccagc ctgggcaaca gagcaagatt ccgtcccaaa acaaacaaac
81001 aaaaaactgt attgaacatg tacagaattt ttttcttgtc attattccct aaacaataca
81061 gtataccaaa atttagcatt tacattgtat taggtatcgt aagtaatcta gagatgattt
81121 aaagtatata ggaggatgtg tataggttat agggaactac tacgccattt tacatcaggg
81181 acttgagcat ccatggattt tgggtatcct cagggagttc tggaaccact ctcccatgtt
81241 taccaaggga catctgcata tacgtcctgc aacctccgcc tcctgggttc aagcgattct
81301 cctgcctcag gctcctgagg agctgggatt acaggtgtgc accactacac caagctaatt
81361 tttgtatttt tagttgagac ggggtttcac cacaatggcc aggctggtct tgaactcctg
81421 acctcaggtg atccacccac ctcggcctcc caaagtactg agattacagg cgtaagccac
81481 catgcctggc ctacacatgt gtcttctggg gaatggccca agcattcatc agcttctgag
81541 gacccaagac tcaaaacagt ttaaggaacc acagctttag ctatttcaga aagtcccaaa
81601 ctagtacagt atgtaagaaa taaagcacac cgtgggccag atagttgact ctttctctct
81661 agtctatctg gaatactcct accaaagaca ttgcctttgt tacaccaatt ccactcccaa
81721 ggacctgcag tgttacccta ctgcagacgg cactgggccc agctcccctg ctgggcatct
81781 aaggccctac agccgcaggt cccatccaca gcagcatcca gttgggctcc ccactttacc
81841 ctgcctgaac ccacacacac caggtctggt gtctctgcat gtgtcctctc tgtgtcctct
81901 ctgcctctgc aagttctgct catccttccc accctgtcca cggctcagct cctccagcag
81961 cctcccggaa acagttatgg gaatcacagg acaKcagagc tacagggagc ctcagagatc
82021 aggccgggaa accaagctat ggggaggagg aacttgccca aagttgccct gtaagtcagt
82081 ggcagaggta taacgagaac tgaggtttcc tgactcgcca tccagtgctc tgctgcactg
82141 acctcccact tctctgaact ctggaggaat ggcaatgact ataaccMcaa cttggcattc
82201 cctgaacacc tgtgtaatgg cgtcagtgtg ccgcattcta acaagaggaa atgctgctgt
82261 ttcaactctg catccattcc cccactgcct ctttccacaa aggagctgag gtggcagatg
82321 ctgctctatt taaggctcat gcgatgcttc ccatgcctgc gacttctcca gactacaagc
82381 ttcagagcac agggaggctg tgttgtcttg gctcaagtgg ctcagcagag acaggcacac
82441 tgtagagcta atgaggaact tactacgtca gttaaagtta tcggtccaaa gtcagagtga
82501 aacactttct gagcagggga tcttgcctcc atggccagca tctcagagcc ctcagggaca
82561 gaacaggaca aggggacaga aggttccagg cgacagaaca taaagagcct ttccaatact
82621 cagagctgcc tgaggcagtg gtgagcctct caacactgaa ggcatgctag ttagccagac
82681 agccacctga gggggccYtg cactgggcac tgacaggtac atggggcaac cagtgaacac
82741 aagcatattt gtcagccaaa cctttgtgaa gggaggcagc agggatcaat atatcagtga
82801 agggctagag cacctattgc tcgcataaca acaattaaag Raaaaaactt aaaagtggga
82861 aggaagggag aatggagaag gtagaaattt aagtcgttta aagaatcttc cagacttttc
82921 acaatggttc ttcaataagt ctgccatttt aagaaagaac agaaggagat atagttagga
82981 aaaaggcagc cagtgttgta aggtgtggaa tgaggatgct gggcagacgc tccagagctg
83041 tacgagagac agagagagag agagagagag agagagagag agagagagag agagagagag
83101 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgcg cgcgcgcgcg cgcatgcgtg
83161 taagtggaaa gttgaacctc cttcctgtgt gctccaagag tctgcacaaa gagYaaatca
83221 cctgtcttac agggccctgg gccttcctaa cccttctttc tcccccgggg ttggaagccc
83281 acagacccaa agggctgagt catagtgtgc ggaggtgcct ggcccctggc agaaggcaac
83341 agggctggca aagagcccag gctttcacaa cggtcgggtg cccgtgtgct ccaagtcggg
83401 gaagtcacta gcccaggaat ttgcatatgt gagcgagaag caccctttgc tcctgcccct
83461 cgtgtatccc caagtgactc caggRtacct aasggatctg aacaggttct gtgcctctca
83521 catggtagca agtgacagga cacaaagagg gcagcaggtc aagcccgggg tcctgcccgt
83581 ccactcacca tcgcattggc atggctgctg ctctctggtg aattctgctc ctcaggagtg
83641 accacttcaa attcagagga ggtgccctgt gcagaaagga agttaggtgc tgcccgagag
83701 aaaaacagct cacccctcct ctgcggccct gtggctgaga gcagcaaggg ggacaaaatc
83761 tcttcccatt ttgagtacac agggaccgtt acatatcatc tcacatRaga tcacaaagca
83821 ggattctcga caagcctgtc atagccctct gcataatgtg aggtcatatc ccctcgatgg
83881 ccaatcctct tttgtagaag ggagatRtgg gactagctga ccttgaacca gcagcactgt
83941 aggattgcat tttttaaggc cgtgtacttt ttaaatgctt tcatgagctc cattatttct
84001 cagggtgacc catggagtgg gtcagaaggt tatatcctct ttggaaagat gaggaaacta
84061 cagtatacag ttggttaatg caaaagcagg gattgaaccc aagactgcag atccagcatt
84121 cctgccacgg ccccagagct gtctcttagg cccagacgtc tctgatcggt ttcccagaac
84181 acagatggca gctgttacgg ccaccaccaa gcatgYcctg tgctggcctt cccaagccag
84241 tcctggagat cccagaggag tgaactgatc tgagaaaagt cccagagatg attcaccaaa
84301 tttggagact atttcaggat tcaaccaaat ccacggtggc caagaaatcc cagatttttt
84361 cacatctttc cgtcattcct agtagaatta ctggtcattc aaccaaccaa gaagccaatg
84421 gcccacctga accagccatt agctctggga tctgggattt ccatgtcccg ttaaacctgg
84481 tctgcagcct cccttcatcc cagaaagggg caggatagat gagaggagtc agtgctcagc
84541 ttccagctga ccctcatggc caccaagaac tcacctctgg ctcttggccc tgccctagaa
84601 agtctccctc ctccatctac attttcccca ttacccgtgt aacctcagaa caggtYcttc
84661 tttccctgca cctgatctgt aggataagga tgctgaacta tgcaatccct tgtaggatac
84721 caagcaaaac ttctccagcc aaatctgtag attttttttt ttttttatga gagggtctga
84781 ctctgtcacc caggctggag tgtggtggca tcaacatggc tcactgcagc cttgacctcc
84841 tgggctcaag cgacccttct gcctcagcca gcctcccaag tagctggaac cacagacata
84901 caccatcaca cctgactact ttttttctta gagagatgga gtctcactat cctgcccagg
84961 ctggtctcaa actcctgggt tcaagcaatc ctcctgcctc agcctcccca agtgctggga
85021 ttataggcat gagccactgc gcccagcctg tagaagatag tttctgagcc ctctttgaga
85081 atgtgagctc catgtcacat ctgtctgttt tcctagcact gtgcacactc agtgatactt
85141 aacagtatac aacttagtaa gtgagaagaa ttccttgtac ataccagggt cttcctggta
85201 agttttaact atgaccttca tttcaaaatg gggaggctga ggccccagag agtttgcgat
85261 ggacttgccc aatatcaccc agcgagacgg aacacagttt cttgacctca accctctttc
85321 ttcatgttct tgtatctgtc agtaggactt gaggtccatc caggcaacct ccacccatga
85381 ctccaaataa aacttacact ggatggaggc ttctggactg gtgctggctt gtcactgggg
85441 catgcagRgg ctgtgggaga tgctgtgaca tttgagtcct ttcctggaga atcaagaaag
85501 cacagatgaa ctgactggga aggggagaag cccaggtacc accctctcaa ggacagccct
85561 tgacaaaagg gttctcagac aggattcccc actcgagtgt gggagaatgg gaggtggtct
85621 catcagggcc aggtagtctg agaaaatggc ctgccctgga gtgacggggc agagcctgag
85681 cagagagacc atgctccctg ggcttactgg gaaaatccca agttggctgc tgctccttgc
85741 tgccctgaac aggcctcaat gataaagaca atgatttaca atttgaaaag tctttatgta
85801 tctattatcc cagccattct cataaaagcc ccatatcccc attttaaagg tcaggacact
85861 gaggcttggg aaggtcatca Mcttgcccac agccacatag ccagcaactg caaactcagg
85921 actgagactt ggccctccct ccctgacttc ctgcagtcct tgctgcaggg agctccttga
85981 aggtagcttc atttctcact ctgagagtgg acctggcacc aatcaggaac tcaggaacat
86041 tgctgaatag acaagcagag gctggaaatg cccactgact gcagcacagt ggactctgag
86101 accatcactt tcctccattt gtccattcag caaatgcaac caccagccaa tagaaggctg
86161 tcacattggc ctgggccaga taccatacag tagagctgca gcaggaggca tctgagtcca
86221 gctgaggtgc ggatcatttc catcctggtc ccagacctta ccctggactt ggagacatgc
86281 cagagtaatg cacactctgt acgcatggtg atgcctctac catacatggg acttcaaccc
86341 cgagccacag tcaccctgtc ctttaatggg cagcacacgt aaccttacat ctgaaatacg
86401 ctgcagcagg ctaacaggca agcccagctt cttccctggg cctcagcttc cctaattgta
86461 aaatggacgc gcagggcacg accagggagc ttccaactca tatgccagct ccaagtgaca
86521 gggagtagct gcttggagYg cgatgcctgg cacacagtag ctgctcatta aatattaaat
86581 atgctgataa agaccccaag gccacaggac tgcaatgtag tcaactgttg tatctattac
86641 tcatgtctgc tggggcctca ggagtgctga gtcactggct atttttaaca agattgtttt
86701 gaagtccctt ccaaatctga ccttctgggg gatgtggcag ccaaaacatg aatggaaggg
86761 tagcctgtga cctaaggata aacgagagaa tgtggRtttt ggcataagaa gggagttcac
86821 tgtgaaggat ttgggcagtt tggggtacag ggagagtcag aggtacccag actcctctta
86881 tacctgtgag ctcagccagg gggtcgaagg agcccaagga gggagaaggt ggtgggagca
86941 gctcgttgtc cttcactagc tcctctgcct tctgccggag cctggtcgct tccatctggg
87001 actcttccaa aagctcccct agagttattg gggaagagga agcaaaagca tttactYggc
87061 tcagtgtgct gcttctcccc gtcccaggcc cctaaccgtg ctgccgcctg gcttctgagg
87121 ctccaggccc tggacttcac tcccaccgtt gctctgtggg ccaagtggga caaactccat
87181 gctggtccac cataagaaac aggctagagc aaggacgagg gcccagcaat cgggggacac
87241 aggagcggaa ggaatctatg agaaactcct cggctccccc aacccccagc attctctttc
87301 tgccccactc ccttcacacc cagccagggc actccacaga aagccagggg ctcccccacc
87361 ctacgggcta cccacagcct gagccaccag ggcaaagggc tgcctgcagg gcggagacct
87421 tggataattc caaccagccc tctaggcctc cattttcctt ctcctacaag tggagatggg
87481 gaaaccactc ctccctcccc agtcggccat cccaagcaag gatctgccaa gcaaggagaa
87541 agcattttaa aaatcagggg gctatgctcc cctctaaaac atgagactag agaagggcac
87601 ggcaaggcaa aggaagagtt tcaggagtgt ggaagaaaga gagccccctc tggatgggga
87661 aatcaatgaa agccttgtct ggaggagaag ggggcacagc aactgggtgc tgatgcacag
87721 ggcccgtcgg tcgtgtggag ggaggtgggg ttgttctaaa gtggggatgg cttgagcaag
87781 agccaggaga agagaactga gggacctacc acctccttgc ttcaagccaa gctctgctcc
87841 ctgctccaac acctaggaac gcgtgcccca gttgcctggt catgctggct ctccccatct
87901 ctcgtgcccc agaaagagga gRccactcta gttccatggc cattaactgg gccttccagc
87961 cccctttccc ttagcccacg cctccctctt ggacctgagt cctgtcatcc tggtttccgg
88021 gtgctgagga atcagggttg ggaatactgt caccatgcta catggtgcat agagacagct
88081 gtgaggcagg agccagcRct gggccaggag ggacagggga tgacttgggc agaggcatat
88141 gccagcttca ttctccactg ctagtagagg gactggcatc acagtctgca gggaggggcg
88201 ctcgcaggga ggggacaggg tctgctttac ctaacatctt tatcccttgc attttttcct
88261 tcagccggga attctccttc actaggcgct caaaagctgc ggatgcctct cctgagggca
88321 cgctgccccc agggtcgtag atccggtacg gtcctctccc ttccatgagg gtagctcagc
88381 ccctgccgtg gtgcccgcct ggctgtaagg acaacagcag catatcagca ggctggccag
88441 gccatggggg catcctttgc agagaagctc tagtctgtca atgccccaga aggcagtccc
88501 ccactttaag ctggtctgac catattatag taaagcagga cagtcacctc cttttatctg
88561 gacgctctgc ctctattaat acaaccttag aatggcatta gtttctctta gcagcctcaa
88621 tgcactgttg gcttgtttgg aacaggagtc caaataaaca gtagggtgca atgtgggata
88681 caagaaagaa cctggacttg gaaaaaaaaa aatctgagtt catgtcctgt ctgtgaggtc
88741 cagaagaaca ggaatcccat atgttttacc catcattgca tcctcagagc ctaccacagt
88801 attagctatg aattttgccc aataaacact tactcaatta atggtctagg cattaattta
88861 ctatgggatt atgggcagtc ccttcccctc tctgagggag ggaaatgaaa gtcttaagtt
88921 aatttagtaa tctttaaagt gaaatgcttg taccctaagg gataagcaag tcaacccagt
88981 ccaaagttcc atttatatgt gtatcatctt ctttaaaaaa agaaaagaaa aaaacaccct
89041 gactaatatt taatacactg tcaccctcct ccgtgagtct catatcatat atggtctgtc
89101 ccttgcagtt ctaaggaaca gaagaatttt tcatcaaacc actgtctatt tgagcaccaa
89161 ggtttttttt tagtaatctg ttcagtttca tctgctttat gtcactgaaa ccagttggaa
89221 aggtgatttt agaatcctaa ctctgtggct tagtagagaa gctctctgtc cattcactca
89281 tccattcaac aattacttat tgagcatcga ctccgtgcKa ggcaccagtc tgggtgctga
89341 ggaattatca gcaaataaaa cagacaaaaa tccttcatgg agcttatagt ctaggggtga
89401 aagcagagga ggatggtggg gaacacaata tttcaggtaa tcaaaaagtc tatgcacaaa
89461 aggccccaag cataaggagc tattttagcc aagacctgaa agaggtgagg gaactagcca
89521 tgaagacatc tgaaggaaga acattctttg cagagagaac agtaagggca gaggccctaa
89581 ggctgaaaca ccgagccagc ctccagccct ccaaatagct ctccagcatg aaagggccaa
89641 ggccttcact ggggacctca gaagggtttg ggggctcRgc accttcctcc caaggtcacc
89701 acccccaaga caaaaactgt ttcatcagga atcctcaaag accctcccaa gtccacacac
89761 catgctccgt gggtccttgg cgctgccagg tgactacgac cataccaccc tgctcccaga
89821 atgtaaatga aagatcagag tgagaaagga ttaacagcaa caccaccatg cttgaatcgc
89881 ctccttgtca ggcactgcat taaatgtttc agatatacaa tcttggagga gctgcacagt
89941 aactctaaca tgcatactaa tctcatctgc attttacaag ggagaaactg aggctcaggg
90001 cggttaagta actcagccac gatgaMccgg ccRcaataag agccaagatg caaagtcagc
90061 cctgcctgct gtgactctga cccccaccat actcacacag ccaggacaca gagScagagg
90121 ggcagacagg gagttcaaat ctctttgtag ggcagcacca tgctatcagg tcacctctgg
90181 gcagtttccc ttgccctgca gcataagaac caataaggac ttgtccaaga gcgtccatag
90241 gatggaatgg gccagaaaga aaaacaaagt tctgatgaga aggacccctt ctcccaaaga
90301 ctgacagata caagatgaat acaccRtgtg ggaggcaccc acgccctgct gagcctgcga
90361 ggcaaaggac agctacccac actcccatca caagtcatca cccggcttag gggtgacttg
90421 tttttaacct gaaaggtcag tgttctacag catagcacaa agYgcagatg tttccaccga
90481 actaagaaac agtgtggggg ctgcacttag cactggaact ggcacatagt aaagtactca
90541 aaaatgtYgg cagacattgg gggtccttcc cctctgcttg cctctgtcct cacgaggctt
90601 gccacctggt acagcatgtg tctgactggt gcgggcattg cccatctggc tgggaacttc
90661 ctgaggcagg agcgtacttt gcacatcttt gtattcctgt cgggagcaca ggagggtggg
90721 ccttccttga gagctgcttg gagttcaaga acagtgaaac agagggctac aaggggcctg
90781 gagccacctt gcatgacaaR ggcagcagtg tcctggttct tgtccaccat tatgcatKca
90841 ccctaaatgg aactcacagt caacaccaca tgaaggcatg gggaatttct tactcaatgt
90901 ctgccttgtc ctgtagatta gaggctgcat gaggacaggg agcaagtcca tcctgtgtgc
90961 cagggtatct tcagagtggc ccagagtagg gcctcggtaa accttgaatt cagagaagtc
91021 agactgaagg gaacaagccc ttgaccccga ccccgcctcc tgcaaggcca tgtccaacat
91081 cccagagaaa gcctaaccct gacccactga ccagacgcac ttggacgccc agacacttct
91141 agaatccagg ctgggccagc catgctccct cagagaggcc cagatgtaag gcgaagggac
91201 aaagcaggat gcaggatgtg ggaactgaaa cccaccccac gtcctccaaa acacgcaaca
91261 ctcagtttct cttggcgctg gggagtcaac gggaaagggg ttcatgggaa acaaaaagtg
91321 ggcagacaga gctatgctcc cctactttag cctcaggggg cagagcttgg ggagaaagaa
91381 aggcagtggg cagaggagaS gcaggagaaa tatcctattg tgggaaaacc ctctctggag
91441 atcagaggag ccacccctgg agggcctgga cttctggagg aaagaaagac aaagagggaa
91501 acagggaatt tccaagttct gctgacctca tgcccagatg gtcagcggca gtccctggat
91561 gaccactggg cagacgggaa ctctccctct gatcaatgag gtttttataa cttctcccag
91621 agagaggact cattctcagg atgacagagc tggaggggac tctccaaggg caatgaggcg
91681 aatcctgcat tatcaggtgg gagagagatg cacccagaaa cactcaatgg atgaggggct
91741 gcattcgaac atgacgatgg catgcactcc cctctgctgt ccacaaacca cgaggatgtt
91801 ttcaaagggg aaaaggaagc actgaccact cccctccatg tggcagtatc tggagttcag
91861 agcccaggag tcctcgggca tgagctcatc tgattctcac tcacggcaca ggcagtgcag
91921 caaggcaagg ccctgagcag ggaaggccaa ggtcacaaag gagtcaggat ggagctggga
91981 ccagaagatc cctgctctcc ctccaggctg cccacagccc atcctccatt tcccttcctg
92041 cccagcaatc ccatggcaca ccacaggccc ccaccacatc ttctcRgagt ctctttccac
92101 ctggcctggt tccacaaact acaaatcagc aggtgctcca ccagcagtgg gactccagcc
92161 ccgcttgccc aaaccagaaa cagctggtgg tatagaagca gccagcgcag actttcttcc
92221 aaaacaagac ctcagccagc ggggcctcca cagcctgccc cagaggggcc ctggggctgc
92281 ccgctggaac cagaaaggcc cagtttgggg gcagaaaggg gcccagacag acggcaggtc
92341 aggcaacaac cacagaccgt gaagatggaa gtgatgctgc tgaaaccgaa ggagccaccc
92401 actagcaggc aagcacttgt gggtggcagg gaggcagtag tccccagggc tcaactctca
92461 gccttacccc agaaagagag gggtatgata aagactacat tcagaattaa atttacattc
92521 tagcttttgc tctgaaatgc tttggtctgt cagattatct tcccattagc agtgttctgg
92581 tggctgggta ggggggtggt cccagacccc ccaaatgata ttcacttgca tagccactaa
92641 catttgcaaa atacttcata gtgtcccaag cattgttttt ccatctgatt atcacaacag
92701 ccctgggaag aaggcagggc tgatacctgc ctgatggata aggaaactga agtccagctt
92761 ggggacttaa tcagcccaat ttaatgcctt tggtcactgg ctgcatcctg gggccagcta
92821 tccctgcaac cctggagcag gtgatgccct ccctggccca gagggaaaag tgtaagcaaa
92881 tctctggtac agggaggtgg aaggtcatcg aggccaacat ctacctcccc agcagaggtg
92941 agggctgcca gggatgctgg caggtgggct ggcagagggg gtatagggaa ggggtctcca
93001 ggaaagacgg cacagaacag aacgtcagag aggtctattc tagtgtctac tctgctgtgt
93061 atggaaccat gggctcattc cctcacatgc aaggggagga gcatgggcca cacagaaacc
93121 gggtgatggc taaagctggc ctcaggatca agaagaccca agtctcagga gctagcagca
93181 agctgaggaa agagagagag ccagcaccat ggaaacatcg ctgccttgga ggcaggaaga
93241 aaggtccaca cagggaaagt gctcaaggcc aaccttgcga acacgaggcc tgagaggggg
93301 cctcacctgc actagcctca gctctgtcca ccctgtgggc tgtgaaaccc tggagcagcc
93361 actcaccttt cctgagcctc aggtgcctca tctgtaaagc aggagtaata atagaaccta
93421 cctgatagaa gatcaaagaa attaaatgaa acaatatgtg tatagttgga agggaattct
93481 cactttctac tttaggcaat acttcctgat tatctgattt aacaagaaac acatctttgt
93541 tttataataa gatttccaaa ataataagag tatacagtaa ctagctcagc atttgacaca
93601 cagcaggtgc ttaataaaag gctgaggcaa ccccacaact gtgcccagct tatctaaggg
93661 ccaaggcttt ccccatttgt tctctccttt aatgggattt taggctcttc cgtcagctca
93721 gtaagctagg ttctggcctc cacttattct ctagtgacct gcatatcacc atttaactgt
93781 ggttaagcag ggtgcctgga cacaccatag ctagtgaaca tgtagctata agaagccctt
93841 gaaccacgat gccacactca ccaacaactg ggaatctgag cctcatttcc tctgctgtaa
93901 agggcaatat cctgcaattc cacccaagga tgaaaggaag tgagaYgcca tgatatcaca
93961 ttgcagacat aaacaaaaaa ctgtccacat atttgtgtta tgatgacctc cagtcctccc
94021 caaccaattt ccaggctggg gaaactgcag cctcaggtcc atccatcaaa ggccgggttg
94081 gatttttcag gcagtgaaac tactctgttc aatactgtaa tggtggacac ctgccattcc
94141 acatttgtcc aaacccatag aacatacacc accaagaggg aacactgatg taaaccatgg
94201 actccgggtg acagtggtgt caatgtagat tcatcaactg taaaaaatct accactctgg
94261 tggaggatgt tgataatggg ggaagctatg cctgttaggg gtgggggggg cgcagtatat
94321 gtaaaatctc tgtaccttct tctcaatttt gctgtgaacc taaaactgct ctataaaaaa
94381 taaggtcttt aatttaaaag agaaaaggac caggctgggc tctcctgatg gctgtcaggt
94441 tcacacatga gtgaggacac agcacatact tgagacagtg tgtattcaag gaccgcaccc
94501 tggcatcctc accaacaagg agcccagact taactcctcc atccttgcaa tcaactgagc
94561 aacaatgagg ctgcagacac agagtctcat atagtaacag ctggggcgcc agaactgtgg
94621 gatctagtca aggtacacta gggcgaacat cttaaccctc actaggcctc agttacctaa
94681 aaagagggag ctgcaaaaat agtaataaca tggggggttg tgcgggataa tcattaaggc
94741 cccttccagt tctaacattt caggatctga aatttattag cactactttc tttaaccttc
94801 taaccttaag aaggctccaa tccccagctc caatcccttc taaggcccag gtggatttcc
94861 ctaacccatc agtcctccca taagagcttt cataggtgct atcccctccc cgagggcagt
94921 cagagaccct cagctgacat ccgcagccct gtgccaggtc ctcccctcct gcctaacttg
94981 taccaggctc tggccaagcc tgatctgtgt tcctcaacat ggcacacatc ttcccatgtc
95041 cttcatcctc ccctcccgag tcacccacaa agaaggcccc actgtctcca ctcagcctcc
95101 gaggcccaga ttacacacca cccgctaaac acctgagcca gaattcacac tgcaaaagag
95161 gactggagag cacctaacta agctggagag agggtgacag ttcgttaggc gctaccgtat
95221 gccaggtgct gggccgcatc atgacgcaca tgacctcatt ttcttcttgc cacacagata
95281 tggaaattat catccacact ttccagcaag gaaaccagtc gacgcagcta catggatgag
95341 gaagcagact cagagaagtY aagtcatttg cctgagaata cacagctggt tagtggtaga
95401 acaaataatg cagcctcaat ctgtcagatt ccaacatcag tgccctttcc actgctctac
95461 actgcctgga gaggcggcag gctgaagctc agaggtggga aaccagacct actttccagt
95521 tctccagaac gtgccccaag ttcctagccc ctgcctttat atgtctctag ctttatatgc
95581 aaccaggtgg caatctgccc cacatcacct gcagtcaaca gggtttagaa tctcaggctg
95641 gaagggagct cagaggacat tccatcctgt gcttacccag tcctctgagg agcccaggga
95701 ctccagctgg ccaactttag caatccagat ctcaccaact tcctccccaa aacaattcct
95761 ggggcagcca gcactggcta cagttaggct gctcccagtt ctgtttccac tggcccttct
95821 ctcccctctg tggtcccctg gaataaggaa caagatgccc atcttcagca tgacagccag
95881 ccctgaggac atttggaacc tgcagccttc tctatactgt tccacatagg gactgccccc
95941 cctccagtcc cccaccaacc ctctacccca cactatctct agttggttct agcactggac
96001 ccaacattcc aggcatggtt tgcctgacca aatgcaggct ttcacctcca cccttttagg
96061 tagtatattc ctattgaggg agcctgggta tacatttgct tttcttacag ctgagtcacc
96121 tactgaagaa tgtcctttct caatgacaac aacctgcatg tatgcctaac tggtgagaac
96181 aaagttctca tgtagaaacg tgtgcttgag ggctgggtgc ggtggctcat gcctgtaatt
96241 ccagcacttt gggaggccga ggtgggcgga tcacctgagg tcaggagttc gagatcagcc
96301 tggccaacat ggcaaaaccc agtctctact aaaaatacaa aaattagcca ggtgtggtgg
96361 cgggtgcctg tagtcccagc tactggggag gctgaggtag gagaatcgct tgaacttggg
96421 aggtggagat tgcagtaagc cgagatcacg ccacagcact ccagcctggg agacagagtg
96481 ggactccatc tcaaaagaaa aaaaaaaaaa aaaaggggtg tttgaggagg tgtgagttac
96541 ctaagggggc agaagtaact gattgattca gtaatMccct atctaggaat ctagagatct
96601 ttgcatcagt acacagaagc tggacaaaaa tatgcactgc cacatgcttt gtaatagaca
96661 tttttgctat tcttgaatgt ccatcaagaa gggactggtt aaacaaacca ccattcattc
96721 Ratggaatac tatacagcca tcaaaaagaa tggggtagat tgcttggcaa tgatacagaa
96781 agatgcccat aaaatacaaa gcaaagtgca gatttatatg tgtaacagga tgctattttt
96841 ttcaaaaaca cattgtgtgt gtgtgtgtat acatacggaa tcttaaatgt gcatagaaaa
96901 aggcctggaa gcccagtgct ttgggaggcc aaggtgggag gattgcttga ggccaggggt
96961 tcaagaccag cctgggcaaa aagcaagaat agattctcta ttaaaaaaaa acttttttac
97021 attagcagag catggtggca catgcctgga gtcccagcta ctcggaaagt tgaggtggga
97081 ggatcacttg agcccagcag ttcaggctac agtgagctat gatcatgcca ctgaactcca
97141 gcctgagtga cagactaata ctctgtctct taaaaaaaag aaaagaagaa aagaaaaaag
97201 tctcaaagga tacataccaa actgctacta gtagactgct ctacagaagg acctcggtaa
97261 ggaactaaaa ccaaaagaaa cttttatttt acctatgcta ttttaactct ttacatggga
97321 aatctagtcc tgttcttata caattataaa attagttttt aaatacagta gggacagagg
97381 aaggggaaag gaaggagaga gaagacaact gtcatttttt acattatatc ttttgtactg
97441 cttgagtttt ggggggtttt tccccaataa tcatgtgcta tttggtaatt ttttttaaag
97501 ggaaatttta aaatattttt ctaaaaaatt taagtgagct gagtgcagtg gtgcatgcct
97561 gtagtcccag ctactccaga gacagacgca ggaggatggc ctgaaaccag agtttatcta
97621 accctgtaac tcccctgcct ccaagaggta agatgccaat tttggggaat tcctcttata
97681 caaaatatgg tggaaagagg gtttcacaga ttcaaatcct ggctatgatc ccttctcgaa
97741 tgaccctggg taggcctcac tttccugtt tctccctatg gaaatgttat gaggaccaag
97801 taggaaccat agagttctct gtaaacctta aaatgctacc cagacaggag cagtaatggc
97861 tgccaaggag ctaggaagga aaaggggctg gacaggggct gaggtactca gatgaaagcc
97921 aaagatgcct tgtgttaaca acaaaaagct gaaagctacc taaaatgaag agaaaaatga
97981 ataaatacat tgtggcaatt tcctatcagg aaatactata cagcagtgaa aaaagaatga
98041 gccacagctg gagatacgaa catgaatgaa tctgggtctc actctgttgc ccaggctgga
98101 gtgcagtggc atgatctcag ttcactgcaa cttccacctc ccagcccaag tcatcctccc
98161 acctLagcct ctcaagtagc tggaactaca ggcatgcgcc accacaccca gctaattttt
98221 gtattgtttg cagagatggg ttttggccat gttgcccaag ctggtctcaa actcctgggc
98281 tcaagagatc cacctgcctg ctctgcctcc caaagWgcca gtaaggcatg agtcaccacg
98341 ctcagcctaa ataacatttt taaacataaa aaaatactat gtattattta gggatgcaca
98401 cattaaaaaa aaaactttaa agaaatgcaa gaaaaaaacc ccactaaatt cagaacactt
98461 gtttcttgtt ggtggctgat gaaggagaaa tttcaaStga ttggaaatgt gttatttctt
98521 aaaccagatg agtgagcatg taggtgcctg cgtgttaccc tcggtatctg agagactcct
98581 tcaatttttc ttttttcttt ttactgtatt gtttgctttt tttctgcttc aacttttatt
98641 ttagattcgg ggggtacatg tgcaggtttg ctacctgggt atactgcgtg atttgggtag
98701 gggtgatccc atcacctggg tactgagcat agttcccaac agtttttcaa ctctgcctcc
98761 ctccctcccc actctagcag tccccagtgt ctactgttgc catctttatg tccatgagga
98821 cctgatattt agctccactt ataagtgaga aaatgcagca tttggttttc tgttcctgtg
98881 ttaatgtgct taggacaata ttttctaaaa gtctatgttg ccagtttcct gaggaccaga
98941 tcacgcagga gttgcacctg cagagacaga agtgggctgg agagggacca gggctcctaa
99001 gtctgctgct gaagctgact atagcgaagg caaaggacaa gtggcctgaa ggccaacaga
99061 gagcctggcc aatgctctgc ccagtgggtg gagactgagc caaaggccct tgactcatag
99121 ggcagtccca tacctgccct ggcctctcag catgactcct tggtgacaca actcccacag
99181 cacccacaca cctacaccca cctaccccac acacacaccc ctgcctgggc tgcccggttt
99241 gtccactgtg taggtgctca aggacgcaga gctcctgcct ctcaaggaac agcctcaggg
99301 tgaggtccct gggagagaag agttcagctc tgcccagagc agccctcaga caaacagcca
99361 gctgctgctc accagtcatc cRttccaatc tgaagagtga ctgtcctggg ccagtcatgg
99421 gaaaatcatc aagaacttcc cRcaatggtc atggtggccg ccaaggtctt tctctggggg
99481 ttgggagctg tgctccccca caaaacaagg ggacccagct gccctgtgcc ttcctgtggc
99541 aactctctgg aggatgcctg gataccctcc ctacagacgg ccagggatag gaggggcagg
99601 cactgcaggc atgtgggggc atagcagcaa gaatctagga gggcaggcac agcaaggcct
99661 ggcccagagg gaaaccagga gctgccagag ccaaagagag ggtgacccaa tcattggttg
99721 ttatagagtt tttaagactt ggaagagaat gccctgagat gccaNagagt gaaacatttt
99781 cattctaaag ctgggaaacc aaagtcggca aaggcgaaca gacgtgccca cagccacatg
99841 ggacagcagt ggcacagaag aaatgcacac ctcgtcctct ggtggccact gtgcactctc
99901 ttaccacacc atgctactgc tcaacctctc cccctccctc acagtggtca ggtaagcaat
99961 gagggaaaat gatggaggtg agaaaggaag aacccgggct

Following is a CETP coding nucleotide sequence (cDNA, SEQ ID NO: 6). >gi|4557442|ref|NM_—000078.1| Homo sapiens cholesteryl ester transfer protein, plasma (CETP), mRNA.

1    gtgaatctct ggggccagga agaccctgct gcccggaaga
     gcctcatgtt ccgtgggggc
61   tgggcggaca tacatatacg ggctccaggc tgaacggctc
     gggccactta cacaccactg
121  cctgataacc atgctggctg ccacagtcct gaccctggcc
     ctgctgggca atgcccatgc
181  ctgctccaaa ggcacctcgc acgaggcagg catcgtgtgc
     cgcatcacca agcctgccct
241  cctggtgttg aaccacgaga ctgccaaggt gatccagacc
     gccttccagc gagccagcta
301  cccagatatc acgggcgaga aggccatgat gctccttggc
     caagtcaagt atgggttgca
361  caacatccag atcagccact tgtccatcgc cagcagccag
     gtggagctgg tggaagccaa
421  gtccattgat gtctccattc agaacgtgtc tgtggtcttc
     aaggggaccc tgaagtatgg
481  ctacaccact gcctggtggc tgggtattga tcagtccatt
     gacttcgaga tcgactctgc
541  cattgacctc cagatcaaca cacagctgac ctgtgactct
     ggtagagtgc ggaccgatgc
601  ccctgactgc tacctgtctt tccataagct gctcctgcat
     ctccaagggg agcgagagcc
661  tgggtggatc aagcagctgt tcacaaattt catctccttc
     accctgaagc tggtcctgaa
721  gggacagatc tgcaaagaga tcaacgtcat ctctaacatc
     atggccgatt ttgtccagac
781  aagggctgcc agcatccttt cagatggaga cattggggtg
     gacatttccc tgacaggtga
841  tcccgtcatc acagcctcct acctggagtc ccatcacaag
     ggtcatttca tctacaagaa
901  tgtctcagag gacctccccc tccccacctt ctcgcccaca
     ctgctggggg actcccgcat
961  gctgtacttc tggttctctg agcgagtctt ccactcgctg
     gccaaggtag ctttccagga
1021 tggccgcctc atgctcagcc tgatgggaga cgagttcaag
     gcagtgctgg agacctgggg
1081 cttcaacacc aaccaggaaa tcttccaaga ggttgtcggc
     ggcttcccca gccaggccca
1141 agtcaccgtc cactgcctca agatgcccaa gatctcctgc
     caaaacaagg gagtcgtggt
1201 caattcttca gtgatggtga aattcctctt tccacgccca
     gaccagcaac attctgtagc
1261 ttacacattt gaagaggata tcgtgactac cgtccaggcc
     tcctattcta agaaaaagct
1321 cttcttaagc ctcttggatt tccagattac accaaagact
     gtttccaact tgactgagag
1381 cagctccgag tccatccaga gcttcctgca gtcaatgatc
     accgctgtgg gcatccctga
1441 ggtcatgtct cggctcgagg tagtgtttac agccctcatg
     aacagcaaag gcgtgagcct
1501 cttcgacatc atcaaccctg agattatcac tcgagatggc
     ttcctgctgc tgcagatgga
1561 ctttggcttc cctgagcacc tgctggtgga tttcctccag
     agcttgagct agaagtctcc
1621 aaggaggtcg ggatggggct tgtagcagaa ggcaagcacc
     aggctcacag ctggaaccct
1681 ggtgtctcct ccagcgtggt ggaagttggg ttaggagtac
     ggagatggag attggctccc
1741 aactcctccc tatcctaaag gcccactggc attaaagtgc
     tgtatccaag
CDS: 131-1612 base pairs
Signal peptide: 131-181 base pairs
Mature peptide: 182-1609 base pairs
PolyA signal: 1771-1776 base pairs

Following is a second PROL4 cDNA sequence (SEQ ID NO:7). >gi|6005801|ref|NM007244.1| Homo sapiens proline rich 4 (lacrimal) (PRR4), mRNA

1   cagagcctcc ttcaagatgc tgctggtcct gctctcagtg
    tccttctgg ctctgagctc
61  agctcagagc acagataatg atgtgaacta tgaagacttt
    actttcacca taccagatgt
121 agaggactca agtcagagac cagatcaggg accccagaga
    cctcctcctg aaggactcct
181 acctagaccc cctggtgata gtggtaacca agatgatggt
    cctcagcaga gaccaccaaa
241 accaggaggc catcaccgcc atcctccccc acctcctttt
    caaaatcagc aacgaccacc
301 ccaacgagga caccgtcaac tctctctacc ccgatttcct
    tctgtcagcc tgcaggaagc
361 atcatcattc ttccggaggg acagaccagc aagacatccc
    caggagcaac cactctggta
421 atctagaatt cagtggcaga aaataaataa gaagataact
    tcattcagaa agccatgaca
481 ttgaaataat gtggtcataa ctctttc
CDS: 17 . . . 421 base pairs
PolyA signal: 442 . . . 447 base pairs

Following is a GRID2 cDNA sequence (SEQ ID NO: 8). >gi|4557632|ref|NM_—001510.1| Homo sapiens glutamate receptor, ionotropic, delta 2 (GRID2), mRNA.

1    atggaagttt tccccttgct cttggttttg tccgtctggt
     ggtctcgaac ctgggactcg
61   gcgaatgcgg attcgatcat tcacatcgga gcaatttttg
     atgaatctgc caaaaaggat
121  gatgaggtat ttcgcactgc ggttggtgac cttaaccaga
     atgaagagat cttacagact
181  gagaaaatca cattttcagt gacgtttgtt gatggcaaca
     accctttcca agcagttcaa
241  gaagcctgtg aacttatgaa tcaaggcatc ttggccctgg
     tcagctccat tggctgcacg
301  tcagcaggat ccctccagtc tttggcagac gccatgcata
     tcccccacct cttcattcag
361  cgctcaacag ctgggacccc aaggagtggc tgtggactca
     cccggagcaa caggaatgat
421  gactacactc tctcagttcg cccacctgtc tacttgcatg
     atgttatcct aagagtggtc
481  acagagtatg cctggcagaa attcattata ttctatgata
     gtgaatacga tatccgtgga
541  atacaggagt tcttggacaa agtctctcag cagggaatgg
     atgttgcact tcagaaggta
601  gaaaacaaca tcaataaaat gattaccact ctctttgaca
     ccatgagaat agaagaactg
661  aatcgctatc gagacactct taggcgagcg atccttgtta
     tgaatcctgc tacagccaaa
721  tccttcatta ctgaggttgt ggagactaat ttggttgctt
     ttgactgtca ctggatcatt
781  ataaatgagg aaataaacga tgtggacgta caggaacttg
     taagaaggtc aattggaagg
841  ttaacgatta ttcggcagac atttccaatt ccccagaaca
     taagtcagcg gtgtttccgt
901  ggcaaccatc gaatatcttc aacattgtgt gatccaaagg
     atccatttgc tcagaatatg
961  gagatttcca acctttacat atatgacacg gtgcttctgc
     ttgctaatgc ttttcataag
1021 aagctggagg accgaaagtg gcacagcatg gcaagtctgt
     catgtatcag aaagaactca
1081 aagccctggc agggtgggcg ctccatgttg gagaccatca
     agaagggtgg agttagtggg
1141 ttgactggag agctagaatt tggagaaaat ggaggcaatc
     ccaatgtcca ctttgaaatc
1201 cttggaacca actatggaga agagcttggc agaggtgttc
     gaaaacttgg gtgctggaat
1261 cctgtcacag gtctgaatgg gtcactgact gacaagaaat
     tggagaataa catgcgtgga
1321 gtggttctac gtgtagtaac tgttctggaa gaaccttttg
     tgatggtctc tgaaaatgtc
1381 ttgtgtaagc cgaagaaata ccagggcttc tccattgatg
     ttttggatgc cttatctaac
1441 tacctgggtt ttaactacga aatttacgta gcaccggatc
     acaaatacgg aagcccacaa
1501 gaagatggga catggaatgg cttggtagga gaacttgtct
     ttaagagagc cgacataggg
1561 atttctgctt taaccatcac tccagatcgt gaaaatgtgg
     tggactttac gacacgttac
1621 atggactact cagtgggggt actacttcga agggctgaaa
     agacagtgga tatgtttgcc
1681 tgtcttgcac catttgatct ctctctatgg gcttgcattg
     ctggcacagt ccttctggtg
1741 ggtctactgg tctacctctt gaactggctt aatcccccac
     gattacaaat gggatcaatg
1801 acgtctacta ctctctacaa ctccatgtgg tttgtgtatg
     gatcttttgt acaacaaggc
1861 ggggaagtcc cgtacacgac tctggctacc cgaatgatga
     tgggggcttg gtggctattt
1921 gctttgattg ttatctcatc ttacacggca aacctcgctg
     ctttcctcac tattacacgc
1981 attgaaagtt ccatccagtc tctccaggac ctttccaagc
     aaacagaaat cccttatggc
2041 acagtcctag actctgcggt atatgagcat gtccgcatga
     aaggactgaa tccttttgag
2101 agggacagca tgtattccca aatgtggcgg atgatcaacc
     gaagcaatgg atcggagaac
2161 aatgttctgg agtcccaggc aggcattcaa aaggtaaaat
     atggaaatta tgctttcgta
2221 tgggatgcag ctgtattgga atatgtggct atctatgacc
     cagattgttc cttttacacc
2281 attggaaata ctgttgctga tcggggatat ggaattgcat
     tacaacatgg cagtccttac
2341 cgagatgttt tttcacaaag gatcctggag cttcagcaga
     atggtgacat ggacatcctg
2401 aagcacaaat ggtggcctaa gaatggccag tgtgacctgt
     actcgtcagt ggacacaaag
2461 cagaaaggag gcgccctgga cataaagagc tttgcagggg
     tcttttgtat cctggctgct
2521 ggaattgtcc tctcctgctt catagccatg ctggagacgt
     ggtggaacaa gaggaaaggc
2581 tcccgggttc catcaaaaga ggatgacaag gaaattgacc
     tggagcacct ccatagacgt
2641 gtaaatagct tgtgcacaga tgacgacagc ccccataaac
     agttttccac ctcgtcaatt
2701 gatttgaccc ctctggacat tgacactttg ccaacacgac
     aagcactgga gcaaatcagt
2761 gatttcagga acactcatat taccacaaca acctttatcc
     cagagcagat ccagactctt
2821 agccgcacac tgtcagctaa agctgcttct ggtttcactt
     ttggcaacgt gcctgagcac
2881 cgaactggcc cttttaggca cagggcacct aatgggggct
     ttttcaggag tcctataaaa
2941 acaatgtcat ctattcctta tcaaccaact cctaccctgg
     ggctcaatct gggtaatgat
3001 ccagaccgag gcacctccat atga

Following is a PDE4D cDNA sequence (SEQ ID NO: 9). >gi|4636198|ref|_—006203.3| Homo sapiens phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila) (PDE4D), mRNA.

1    ggaattcatc tgtaaaaatc actacatgta acgtaggaga
     caagaaaaat attaatgaca
61   gaagatctgc gaacatgatg cacgtgaata attttccctt
     tagaaggcat tcctggatat
121  gttttgatgt ggacaatggc acatctgcgg gacggagtcc
     cttggatccc atgaccagcc
181  caggatccgg gctaattctc caagcaaatt ttgtccacag
     tcaacgacgg gagtccttcc
241  tgtatcgatc cgacagcgat tatgacctct ctccaaagtc
     tatgtcccgg aactcctcca
301  ttgccagtga tatacacgga gatgacttga ttgtgactcc
     atttgctcag gtcttggcca
361  gtctgcgaac tgtacgaaac aactttgctg cattaactaa
     tttgcaagat cgagcaccta
421  gcaaaagatc acccatgtgc aaccaaccat ccatcaacaa
     agccaccata acagaggagg
481  cctaccagaa actggccagc gagaccctgg aggagctgga
     ctggtgtctg gaccagctag
541  agaccctaca gaccaggcac tccgtcagtg agatggcctc
     caacaagttt aaaaggatgc
601  ttaatcggga gctcacccat ctctctgaaa tgagtcggtc
     tggaaatcaa gtgtcagagt
661  ttatatcaaa cacattctta gataagcaac atgaagtgga
     aattccttct ccaactcaga
721  aggaaaagga gaaaaagaaa agaccaatgt ctcagatcag
     tggagtcaag aaattgatgc
781  acagctctag tctgactaat tcaagtatcc caaggtttgg
     agttaaaact gaacaagaag
841  atgtccttgc caaggaacta gaagatgtga acaaatgggg
     tcttcatgtt ttcagaatag
901  cagagttgtc tggtaaccgg cccttgactg ttatcatgca
     caccattttt caggaacggg
961  atttattaaa aacatttaaa attccagtag atactttaat
     tacatatctt atgactctcg
1021 aagaccatta ccatgctgat gtggcctatc acaacaatat
     ccatgctgca gatgttgtcc
1081 agtctactca tgtgctatta tctacacctg ctttggaggc
     tgtgtttaca gatttggaga
1141 ttcttgcagc aatttttgcc agtgcaatac atgatgtaga
     tcatcctggt gtgtccaatc
1201 aatttctgat caatacaaac tctgaacttg ccttgatgta
     caatgattcc tcagtcttag
1261 agaaccatca tttggctgtg ggctttaaat tgcttcagga
     agaaaactgt gacattttcc
1321 agaatttgac caaaaaacaa agacaatctt taaggaaaat
     ggtcattgac atcgtacttg
1381 caacagatat gtcaaaacac atgaatctac tggctgattt
     gaagactatg gttgaaacta
1441 agaaagtgac aagctctgga gttcttcttc ttgataatta
     ttccgatagg attcaggttc
1501 ttcagaatat ggtgcactgt gcagatctga gcaacccaac
     aaagcctctc cagctgtacc
1561 gccagtggac ggaccggata atggaggagt tcttccgcca
     aggagaccga gagagggaac
1621 gtggcatgga gataagcccc atgtgtgaca agcacaatgc
     ttccgtggaa aaatcacagg
1681 tgggcttcat agactatatt gttcatcccc tctgggagac
     atgggcagac ctcgtccacc
1741 ctgacgccca ggatattttg gacactttgg aggacaatcg
     tgaatggtac cagagcacaa
1801 tccctcagag cccctctcct gcacctgatg acccagagga
     gggccggcag ggtcaaactg
1861 agaaattcca gtttgaacta actttagagg aagatggtga
     gtcagacacg gaaaaggaca
1921 gtggcagtca agtggaagaa gacactagct gcagtgactc
     caagactctt tgtactcaag
1981 actcagagtc tactgaaatt ccccttgatg aacaggttga
     agaggaggca gtaggggaag
2041 aagaggaaag ccagcctgaa gcctgtgtca tagatgatcg
     ttctcctgac acgtaacagt
2101 gcaaaaactt tcatgccttt ttttttttta agtagaaaaa
     ttgtttccaa agtgcatgtc
2161 acatgccaca accacggtca cacctcactg tcatctgcca
     ggacgtttgt tgaacaaaac
2221 tgaccttgac tactcagtcc agcgctcagg aatatcgtaa
     ccagtttttt cacctccatg
2281 tcatccgagc aaggtggaca tcttcacgaa cagcgttttt
     aacaagattt cagcttggta
2341 gagctgacaa agcagataaa atctactcca aattattttc
     aagagagtgt gactcatcag
2401 gcagcccaaa agtttattgg acttggggtt tctattcctt
     tttatttgtt tgcaatattt
2461 tcagaagaaa ggcattgcac agagtgaact taatggacga
     agcaacaaat atgtcaagaa
2521 caggacatag cacgaatctg ttaccagtag gaggaggatg
     agccacagaa attgcataat
2581 tttctaattt caagtcttcc tgatacatga ctgaatagtg
     tggttcagtg agctgcactg
2641 acctctacat tttgtatgat atgtaaaaca gattttttgt
     agagcttact tttattatta
2701 aatgtattga ggtattatat ttaaaaaaaa ctatgttcag
     aacttcatct gccactggtt
2761 atttttttct aaggagtaac ttgcaagttt tcagtacaaa
     tctgtgctac actggataaa
2821 aatctaattt atgaatttta cttgcacctt atagttcata
     gcaattaact gatttgtagt
2881 gattcattgt ttgttttata taccaatgac ttccatattt
     taaaagagaa aaacaacttt
2941 atgttgcagg aaaccctttt tgtaagtctt tattatttac
     tttgcatttt gtttcactct
3001 ttccagataa gcagagttgc tcttcaccag tgtttttctt
     catgtgcaaa gtgactattt
3061 gttctataat acttttatgt gtgttatatc aaatgtgtct
     taagcttcat gcaaactcag
3121 tcatcagttc gtgttgtctg aagcaagtgg gaaatatata
     aatacccagt agctaaaatg
3181 gtcagtcttt tttagatgtt ttcctactta gtatctccta
     ataacgtttt gctgtgtcac
3241 tagatgttca tttcacaagt gcatgtcttt ctaataatcc
     acacatttca tgctctaata
3301 atccacacat ttcatgctca tttttattgt ttttacagcc
     agttatagca agaaaaaggt
3361 ttttcccctt gtgctgcttt ataatttagc gtgtgtctga
     accttatcca tgtttgctag
3421 atgaggtctt gtcaaatata tcactaccat tgtcaccggt
     gaaaagaaac aggtagttaa
3481 gttagggtta acattcattt caaccacgag gttgtatatc
     atgactagct tttactcttg
3541 gtttacagag aaaagttaaa caaccaacta ggcagttttt
     aagaatatta acaatatatt
3601 aacaaacacc aatacaacta atcctatttg gttttaatga
     tttcaccatg ggattaagaa
3661 ctatatcagg aacatccctg agaaacggct ttaagtgtag
     caactactct tccttaatgg
3721 acagccacat aacgtgtagg aagtccttta tcacttatcc
     tcgatccata agcatatctt
3781 gcagagggga actacttctt taaacacatg gagggaaaga
     agatgatgcc actggcacca
3841 gagggttagt actgtgatgc atcctaaaat atttattata
     ttggtaaaaa ttctggttaa
3901 ataaaaaatt agagatcact cttggctgat ttcagcacca
     ggaactgtat tacagtttta
3961 gagattaatt cctagtgttt acctgattat agcagttggc
     atcatggggc atttaattct
4021 gactttatcc ccacgtcagc cttaataaag tcttctttac
     cttctctatg aagactttaa
4081 agcccaaata atcatttttc acattgatat tcaagaattg
     agatagatag aagccaaagt
4141 gggtatctga caagtggaaa atcaaacgtt taagaagaat
     tacaactctg aaaagcattt
4201 atatgtggaa cttctcaagg agcctcctgg ggactggaaa
     gtaagtcatc agccaggcaa
4261 atgactcatg ctgaagagag tccccatttc agtcccctga
     gatctagctg atgcttagat
4321 cctttgaaat aaaaattatg tctttataac tctgatcttt
     tacataaagc agaagaggaa
4381 tcaactagtt aattgcaagg tttctactct gtttcctctg
     taaagatcag atggtaatct
4441 ttcaaataag aaaaaaataa agacgtatgt ttgaccaagt
     agtttcacaa gaatatttgg
4501 gaacttgttt cttttaattt tatttgtccc tgagtgaagt
     ctagaaagaa aggtaaagag
4561 tctagagttt attcctcttt ccaaaacatt ctcattcctc
     tcctccctac acttagtatt
4621 tcccccacag agtgcctaga atcttaataa tgaataaaat
     aaaaagcagc aatatgtcat
4681 taacaaatcc agacctgaaa gggtaaaggg tttataactg
     cactaataaa gagaggctct
4741 ttttttttct tccagtttgt tggtttttaa tggtaccgtg
     ttgtaaagat acccactaat
4801 ggacaatcaa attgcagaaa aggctcaata tccaagagac
     agggactaat gcactgtaca
4861 atctgcttat ccttgccctt ctctcttgcc aaagtgtgct
     tcagaaatat atactgcttt
4921 aaaaaagaat aaaagaatat ccttttacaa gtggctttac
     atttcctaaa atgccataag
4981 aaaatgcaat atctgggtac tgtatgggga aaaaaatgtc
     caagtttgtg taaaaccagt
5041 gcatttcagc ttgcaagtta ctgaacacaa taatgctgtt
     ttaattttgt tttatatcag
5101 ttaaaattca caataatgta gatagaacaa attacagaca
     aggaaagaaa aaacttgaat
5161 gaaatggatt ttacagaaag ctttatgata atttttgaat
     gcattattta ttttttgtgc
5221 catgcatttt ttttctcacc aaatgacctt acctgtaata
     cagtcttgtt tgtctgttta
5281 caaccatgta tttattgcaa tgtacatact gtaatgttaa
     ttgtaaatta tctgttctta
5341 ttaaaacatc atcccatgat ggggtggtgt tgatatattt
     ggaaactctt ggtgagagaa
5401 tgaatggtgt gtatacatac tctgtacatt tttcttttct
     cctgtaatat agtcttgtca
5461 ccttagagct tgtttatgga agattcaaga aaactataaa
     atacttaaag atatataaat
5521 ttaaaaaaac atagctgcag gtctttggtc ccagggctgt
     gccttaactt taaccaatat
5581 tttcttctgt tttgctgcat ttgaaaggta acagtggagc
     tagggctggg cattttacat
5641 ccaggctttt aattgattag aattctgcca ataggtggat
     tttacaaaac cacagacaac
5701 ctctgaaaga ttctgagacc cttttgagac agaagctctt
     aagtacttct tgccagggag
5761 cagcactgca tgtgtgatgg ttgtttgcca tctgttgatc
     aggaactact tcagctactt
5821 gcatttgatt atttcctttt tttttttttt taactcggaa
     acacaactgg gggaat

Following is a GPX3 cDNA sequence (SEQ ID NO: 10). >gi|6006000|ref|NM_—002084.2| Homo sapiens glutathione peroxidase 3 (plasma) (GPX3), mRNA.

1    agccaaaaga ggaagggacc ggcctcccac gtccacaggg
     acctgacttc cacctctctg
61   cccagatttg cttatgtcac tgtcgccccg ggacggggag
     gtggggagct gagggcaagt
121  cgcgcccgcc cctgaaatcc cagccgccta gcgattggct
     gcaagggtct cggcttggcc
181  gcggattaat cacacccgag ggcttgaaag gtggctggga
     gcgccggaca cctcagacgg
241  acggtggcca gggatcaggc agcggctcag gcgaccctga
     gtgtgccccc accccgccat
301  ggcccggctg ctgcaggcgt cctgcctgct ttccctgctc
     ctggccggct tcgtctcgca
361  gagccgggga caagagaagt cgaagatgga ctgccatggt
     ggcataagtg gcaccattta
421  cgagtacgga gccctcacca ttgatgggga ggagtacatc
     cccttcaagc agtatgctgg
481  caaatacgtc ctctttgtca acgtggccag ctactgaggc
     ctgacgggcc agtacattga
541  actgaatgca ctacaggaag agcttgcacc attcggtctg
     gtcattctgg gctttccctg
601  caaccaattt ggaaaacagg aaccaggaga gaactcagag
     atccttccta ccctcaagta
661  tgtccgacca ggtggaggct ttgtccctaa tttccagctc
     tttgagaaag gggatgtcaa
721  tggagagaaa gagcagaaat tctacacttt cctaaagaac
     tcctgtcctc ccacctcgga
781  gctcctgggt acatctgacc gcctcttctg ggaacccatg
     aaggttcacg acatccgctg
841  gaactttgag aagttcctgg tggggccaga tggtataccc
     atcatgcgct ggcaccaccg
901  gaccacggtc agcaacgtca agatggacat cctgtcctac
     atgaggcggc aggcagccct
961  gggggtcaag aggaagtaac tgaaggccgt ctcatcccat
     gtccaccatg taggggaggg
1021 actttgttca ggaagaaatc cgtgtctcca accacactat
     ctacccatca cagacccctt
1081 tcctatcact caaggcccca gcctggcaca aatggatgca
     tacagttctg tgtactgcca
1141 ggcatgtggg tgtgggtgca atgtgggtgt ttacacacat
     gcctacaggt atgcgtgatt
1201 gtgtgtgtgt gcatgggtgt acagccacgt gtctacctat
     gtgtctttct gggaatgtgt
1261 accatctgtg tgcctgcagc tgtgtagtgc tggacagtga
     caaccctttc tctccagttc
1321 tccactccaa tgataatagt tcacttatac ctaaacccaa
     aggaaaaacc agctctaggt
1381 ccaattgttc tgctctaact gatacctcaa ccttggggcc
     agcatctccc actgcctcca
1441 aatattagta actatgactg acgtccccag aagtttctgg
     gtctaccaca ctccccaacc
1501 ccccactcct acttcctgaa gggccctccc aaggctacat
     ccccacccca cagttctccc
1561 tgagagagat caacctccct gagatcaacc aaggcagatg
     tgacagcaag ggccacggac
1621 cccatggcag gggtggcgtc ttcatgaggg aggggcccaa
     agcccttgtg ggcggacctc
1681 ccctgagcct gtctgagggg ccagccctta gtgcattcag
     gctaaggccc ctgggcaggg
1741 atgccacccc tgctccttcg gaggacgtgc cctcacccct
     cactggtcca ctggcttgag
1801 actcaccccg tctgcccagt aaaagccttt ctgcagcaaa
     aaaaaaaaaa aaaaaa
CDS: 299-979 base pairs
Signal peptide: 299-358 base pairs
Mature peptide: 359-976 base pairs
PolyA signal: 1838 base pairs

Following is a CETP amino acid sequence (SEQ ID NO: 11). >gi|4557443|ref_NP900069.1| cholesteryl ester transfer protein, plasma precursor [Homo sapiens]

MLAATVLTLALLGNAHACSKGTSHEAGIVCRITKPALLVLNHETAKVIQT
AFQRASYPDITGEKAMMLLGQVKYGLHNIQISHLSIASSQVELVEAKSID
VSIQNVSVVFKGTLKYGYTTAWWLGIDQSIDFEIDSAIDLQINTQLTCDS
GRVRTDAPDCYLSFHKLLLHLQGEREPGWIKQLFTNFISFTLKLVLKGQI
CKEINVISNIMADFVQTRAASILSDGDIGVDISLTGDPVITASYLESHHK
GHFIYKNVSEDLPLPTFSPTLLGDSRMLYFWFSERVFHSLAKVAFQDGRL
MLSLMGDEFKAVLETWGFNTNQEIFQEVVGGFPSQAQVTVHCLKMPKISC
QNKGVVVNSSVMVKFLFPRPDQQHSVAYTFEEDIVTTVQASYSKKKLFLS
LLDFQITPKTVSNLTESSSESIQSFLQSMITAVGIPEVMSRLEVVFTALM
NSKGVSLFDIINPEIITRDGFLLLQMDFGFPEHLLVDFLQSLS
Protein: 1 . . . 493 amino acids
Signal peptide: 1-17 amino acids
Mature peptide: 18-493 amino acids

Following is a second PROL4 amino acid sequence (SEQ ID NO: 12). >gi|6005802|ref|NP_—009175.1| proline rich 4 (lacrimal) [Homo sapiens].

MLLVLLSVVLLAISSAQSTDNDVNYEDFTFTIPDVEDSSQRPDQGPQRPP
PEGLLPRPPGDSGNQDDGPQQRPPKPGGHHRHPPPPPFQNQQRPPQRGHR
QLSLPRFPSVSLQEASSFFRRDRPARHPQEQPLW
Protein: 1 . . . 134 amino acids
Signal peptide: 1-16 amino acids
Mature peptide: 17-134 amino acids

Following is a GRID2 amino acid sequence (SEQ ID NO: 13). >gi|4557633|ref|NP_—001501.1| glutamate receptor, ionotropic, delta 2 [Homo sapiens].

MEVFPLLLVLSVWWSRTWDSANADSIIHIGAIFDESAKKDDEVFRTAVGD
LNQNEEILQTEKITFSVTFVDGNNPFQAVQEACELMNQGILALVSSIGCT
SAGSLQSLADAMHIPHLFIQRSTAGTPRSGCGLTRSNRNDDYTLSVRPPV
YLHDVILRVVTEYAWQKFIIFYDSEYDIRGIQEFLDKVSQQGMDVALQKV
ENNINKMITTLFDTMRIEELNRYRDTLRRAILVMNPATAKSFITEVVETN
LVAFDCHWIIINEEINDVDVQELVRRSIGRLTIIRQTFPIPQNISQRCFR
GNHRISSTLCDPKDPFAQNMEISNLYIYDTVLLLANAFHKKLEDRKWHSM
ASLSCIRKNSKPWQGGRSMLETIKKGGVSGLTGELEFGENGGNPNVHFEI
LGTNYGEELGRGVRKLGCWNPVTGLNGSLTDKKLENNMRGVVLRVVTVLE
EPFVMVSENVLCKPKKYQGFSIDVLDALSNYLGFNYEIYVAPDHKYGSPQ
EDGTWNGLVGELVFKRADIGISALTITPDRENVVDFTTRYMDYSVGVLLR
RAEKTVDMFACLAPFDLSLWACIAGTVLLVGLLVYLLNWLNPPRLQMGSM
TSTTLYNSMWFVYGSFVQQGGEVPYTTLATRMMMGAWWLFALIVISSYTA
NLAAFLTITRIESSIQSLQDLSKQTEIPYGTVLDSAVYEHVRMKGLNPFE
RDSMYSQMWRMINRSNGSENNVLESQAGIQKVKYGNYAFVWDAAVLEYVA
IYDPDCSFYTIGNTVADRGYGIALQHGSPYRDVFSQRILELQQNGDMDIL
KHKWWPKNGQCDLYSSVDTKQKGGALDIKSFAGVFCILAAGIVLSCFIAM
IETWWNKRKGSRVPSKEDDKEIDLEHLHRRVNSLCTDDDSPHKQFSTSSI
DLTPLDIDTLPTRQALEQISDFRNTHITTTTFIPEQIQTLSRTLSAKAAS
GFTFGNVPEHRTGPFRHRAPNGGFFRSPIKTMSSIPYQPTPTLGLNLGND
PDRGTSI

Following is a PDE4D amino acid sequence (SEQ ID NO: 14). >gi|32306513|ref|NP_—006194.2| cAMP-specific phosphodiesterase 4D [Homo sapiens]

MMHVNNFPFRRHSWICFDVDNGTSAGRSPLDPMTSPGSGLILQANFVHSQ
RRESFLYRSDSDYDLSPKSMSRNSSIASDIHGDDLIVTPFAQVLASLRTV
RNNFAALTNLQDRAPSKRSPMCNQPSINKATITEEAYQKLASETLEELDW
CLDQLETLQTRHSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEFISNT
FLDKQHEVEIPSPTQKEKEKKKRPMSQISGVKKLMHSSSLTNSSIPRFGV
KTEQEDVLAKELEDVNKWGLHVFRIAELSGNRPLTVIMHTIFQERDLLKT
FKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTPALEAV
FTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHL
AVGFKLLQEENCDIFQNLTKKQRQSLRKMVIDIVLATDMSKHMNLLADLK
TMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNPTKPLQLYRQWTD
RIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETW
ADLVHPDAQDILDTLEDNREWYQSTIPQSPSPAPDDPEEGRQGQTEKFQF
ELTLEEDGESDTEKDSGSQVEEDTSCSDSKTLCTQDSESTEIPLDEQVEE
EAVGEEEESQPEACVIDDRSPDT

Following is a GPX3 amino acid sequence (SEQ ID NO: IS). >gi|6006001|ref|NP_—002075.2| plasma glutathione peroxidase 3 precursor [Homo sapiens]

MARLLQASCLLSLLLAGFVSQSRGQEKSKMDCHGGISGTIYEYGALTIDG
EEYIPFKQYAGKYVLFVNVASYUGLTGQYIELNALQEELAPFGLVILGFP
CNQFGKQEPGENSEILPTLKYVRPGGGFVPNFQLFEKGDVNGEKEQKFYT
FLKNSCPPTSELLGTSDRLFWEPMKVHDIRWNFEKFLVGPDGIPIMRWHH
RTTVSNVKMDILSYMRRQAALGVKRK

Modifications may be made to the foregoing without departing from the basic aspects of the invention. Although the invention has been described in substantial detail with reference to one or more specific embodiments, those of skill in the art will recognize that changes may be made to the embodiments specifically disclosed in this application, yet these modifications and improvements are within the scope and spirit of the invention, as set forth in the claims which follow. Also, citation of the above publications or documents is not intended as an admission that any of the foregoing is pertinent prior art, nor does it constitute any admission as to the contents or date of these publications or documents. The content of each patent, patent application and other publication and document referenced is incorporated herein by reference in its entirety, including drawings, tables and cited documents.

Claims

1. A method for identifying a subject at risk of low bone mineral density (BMD), which comprises detecting the presence or absence of one or more polymorphic variations associated with low BMD in a nucleic acid sample from a subject, wherein the one or more polymorphic variations are detected in a nucleotide sequence in SEQ ID NOs:1-5, a substantially identical sequence thereof or a fragment of the foregoing; whereby the presence of the polymorphic variation is indicative of the subject being at risk of low BMD.

2. The method of claim 1, which further comprises obtaining the nucleic acid sample from the subject.

3. The method of claim 1, wherein the one or more polymorphic variations are detected at one or more positions selected from the group consisting of rs7500979, rs2217332, rs8044804, rs2270835, rs2133783, rs247609, rs952440, rs881598, rs2291955, rs2518054, rs866038, rs1436425, rs173537, rs247611, rs166017, rs173538, rs193694, rs7205692, rs8048746, rs247618, rs183130, rs6499863, rs4783961, rs3816117, rs711752, rs708272, rs1864163, rs4369653, rs1864165, rs891141, rs891143, rs7205804, rs5885, rs1532625, rs1532624, rs289712, rs7499892, rs5883, rs289714, rs158480, rs289717, rs4344729, rs289718, rs289719, rs2033254, rs4784744, rs291044, rs8053613, rs5881, rs5880, rs7198026, rs5882, rs8045701, rs289741, rs1801706, rs289742, rs289743, rs289746, rs172337, rs289747, rs1566439, rs7205459, rs289749, rs289751, rs8059220, rs8058353, rs289735, rs289737, rs291042, rs1875236, rs821466, rs821465, rs4275846, rs289707, rs821463, rs289706, rs1167741, rs2052880, rs1167742, rs1183256, rs1651665, rs1651666, rs4784751, rs1651667, rs8052091, rs1684574, rs1684575, rs1672865, rs821470, rs1549669, rs291040 and rs289754.

4. The method of claim 1, wherein the one or more polymorphic variations are detected at one or more positions selected from the group consisting of rs166017, rs193694, rs7205804, rs1801706, rs7205459 and rs821465.

5. The method of claim 1, wherein the one or more polymorphic variations are detected in a region spanning positions 14,328 to 68,805 in SEQ ID NO: 1.

6. The method of claim 1, wherein the one or more polymorphic variations are detected at one or more positions selected from the group consisting of rs523051, rs693620, rs2588349, rs2588350, rs619381, rs3759252, rs3759251, rs2418107, rs7303054, rs1838345, rs620878, rs2537817, rs1548803, rs667123, rs1838346, rs2159903, rs3944035, rs3741845, rs2110096, rs759055, rs589377, rs7960194, rs7978242, rs601051, rs4262797, rs2215714, rs1373434, rs2215715, rs612456, rs612808, rs689118, rs597468, rs592864, rs640372, rs7966559, rs654834, rs4763216, rs668521, rs669503, rs3906864, rs3906863, rs7957888, rs9300230, rs7306214, rs763839, rs2418105, rs666841, rs3851578, rs7138797, rs7295252, rs2418106, rs7299578, rs621112, rs3863320, rs1373432, rs1047699, rs1063193, rs2232959, rs2227296, rs1548804, rs2232958, rs2232957, rs2232956, rs1972571, rs3759250, rs3759249, rs1541525, rs2098248, rs2900550, rs7302130, rs4763583, rs4360778, rs1607695, rs1607694, rs2192139, rs7978300, rs7397871, rs4763217, rs2159900, rs10772370, rs7398682, rs2900551, rs2900552, rs2418214, rs2418215, rs965243, rs1117548, rs1520225, rs1520226, rs1520227, rs971919, rs2159901, rs2159902, rs2110099, rs7314847, rs7296003, rs4281556, rs4763219, rs3851579, rs3851580, rs1049119, rs2298866, rs2298865, rs2298864, rs2298863, rs3180393, rs2070837, rs7956204, rs2418216, rs3741844, rs4262798, rs2418217, rs2418218, rs7137492, rs2110100, rs1013312, rs4579993, rs1013313, rs7397106, rs2215716, rs2192140, rs4763589, rs1468697, rs2070837, rs3180393 and rs2298865.

7. The method of claim 1, wherein the one or more polymorphic variations are detected at one or more positions selected from the group consisting of rs2588350, rs619381, rs620878, rs759055, rs4262797, rs612808, rs3906863, rs7957888, rs763839, rs2418105, rs666841, rs3851578, rs7299578, rs621112, rs1047699, rs1548804, rs2232956, rs1520227 and rs2215716.

8. The method of claim 1, wherein the one or more polymorphic variations are detected in a region spanning positions 2,424 to 93,715 in SEQ ID NO: 2.

9. The method of claim 1, wherein the one or more polymorphic variations are detected at one or more positions selected from the group consisting of rs1433661, rs1485009, rs7681947, rs1816432, rs1485018, rs1485017, rs7438397, rs6834311, rs1368717, rs1017391, rs2870701, rs7679839, rs1385404, rs1368716, rs4693316, rs1905707, rs1905708, rs1905709, rs3912442, rs2082553, rs6831638, rs5860329, rs2870702, rs2870703, rs1948016, rs6835836, rs1994253, rs1905710, rs1485019, rs978191, rs1385405, rs7694361, rs1905711, rs1905734, rs1485012, rs1485013, rs4692981, rs7670552, rs7670932, rs7688091, rs7440540, rs2171000, rs2870704, rs7655758, rs7661436, rs7662289, rs7667044, rs7691929, rs5860330, rs901013, rs901012, rs901011, rs1948018, rs2870705, rs1948017, rs1905733, rs1385408, rs1385409, rs1385410, rs1485026, rs1485027, rs2904483, rs1385406, rs1905732, rs2046418, rs2200377, rs1905731, rs1905730, rs975713, rs6820985, rs7670441, rs6810794, rs7676623, rs1154861, rs1032125, rs1485022, rs1485024, rs3913651, rs4693319, rs1872383, rs2200376, rs7668090, rs7692930, rs967096, rs6822249, rs6532405, rs1017897, rs7672674, rs7694568, rs2904484, rs7340830, rs1485033, rs2870706, rs1905729, rs4693320, rs6848749, rs6532406, rs6532407, rs1905728, rs6819866, rs1905727, rs7674069, rs1905724, rs1905723, rs1485020 and rs6814101.

10. The method of claim 1, wherein the one or more polymorphic variations are detected at one or more positions selected from the group consisting of rs1433661, rs7679839, rs1368716, rs1905707, rs1905708, rs1994253, rs1485019, rs1905734, rs1485012, rs7670552, rs7691929, rs1948018, rs1948017, rs1485024, rs7694568, rs4693320, rs6848749, rs6532406, rs6532407 and rs6819866.

11. The method of claim 1, wherein the one or more polymorphic variations are detected in a region spanning positions 206 to 90,969 in SEQ ID NO: 3.

12. The method of claim 1, wherein the one or more polymorphic variations are detected at one or more positions selected from the group consisting of rs6886495, rs6450498, rs1472456, rs4700315, rs4700316, rs7714708, rs7710479, rs2968013, rs2968014, rs2968015, rs1391648, rs2055297, rs2055296, rs3989138, rs4700317, rs2036220, rs7727206, rs7723432, rs1546221, rs4479801, rs4395595, rs4395596, rs4699932, rs2936201, rs7356672, rs2936200, rs1909296, rs7703131, rs7445308, rs3087748, rs4321723, rs2968016, rs5868151, rs1874858, rs1874857, rs7712922, rs4631140, rs4469166, rs1078369, rs1078368, rs2968006, rs2968005, rs2936190, rs2409613, rs4415048, rs2968004, rs2968003, rs2968002, rs2936191, rs1498610, rs6874662, rs3060393, rs7729722, rs7733884, rs7714489, rs7735570, rs2936193, rs2291851, rs2291852, rs1498602, rs1995166, rs1498603, rs1498604, rs1498605, rs1948651, rs4699934, rs4700319, rs2279737, rs7720361, rs7706419, rs1006431, rs1353747, rs1498606, rs1353748, rs1553113, rs2968012, rs2968011, rs1498608, rs2936189, rs1498609, rs2968019, rs6891238, rs2968010, rs2968009, rs2936203, rs1498601, rs1498600, rs1498599, rs2936202, rs7730070, rs6450501, rs6450502, rs6889456, rs6894618, rs7706044, rs7707541, rs7712076, rs6892860, rs6867053, rs7737269, rs6864156, rs950447, rs2936196, rs7719347, rs1391649, rs1391650, rs1391651, rs1353749, rs10682149, rs5868153, rs1363882, rs2409626, rs2968018, rs954740, rs986067, rs6869400, and rs5010782.

13. The method of claim 1, wherein the one or more polymorphic variations are detected at one or more positions selected from the group consisting of rs7714708, rs1498602, rs4699934, rs1006431, rs1353747, rs1498608, rs1498609, rs2968010, rs2936202 and rs1391649.

14. The method of claim 1, wherein the one or more polymorphic variations are detected in a region spanning positions 1,599 to 82,591 in SEQ ID NO: 4.

15. The method of claim 1, wherein the one or more polymorphic variations are detected at one or more positions selected from the group consisting of rs1478398, rs1478397, rs1160114, rs1160113, rs1382323, rs1160112, rs7709870, rs7710643, rs7730467, rs6579829, rs6579830, rs6579831, rs6896232, rs1351131, rs1038074, rs1478396, rs6880512, rs4958858, rs4958431, rs4958432, rs6898463, rs4958859, rs4130064, rs4130065, rs4133119, rs4958860, rs4958861, rs4437356, rs4958868, rs1478400, rs6889375, rs1600159, rs6875892, rs4608909, rs2345000, rs4516840, rs2054440, rs707141, rs707142, rs841236, rs707143, rs707144, rs6869405, rs707145, rs707146, rs707148, rs707150, rs5872184, rs3763015, rs2042235, rs3763013, rs2042236, rs1946234, rs1946235, rs1946236, rs8177402, rs8177403, rs8177404, rs8177405, rs8177406, rs8177407, rs8177408, rs8177409, rs6888961, rs8177410, rs8177411, rs8177412, rs8177413, rs870407, rs870406, rs6873202, rs8177414, rs8177415, rs3805435, rs8177416, rs3792799, rs3792798, rs3828599, rs8177417, rs3792797, rs8177418, rs8177419, rs8177420, rs8177421, rs4958872, rs3792796, rs8177422, rs8177423, rs4958434, rs8177424, rs8177425, rs8177426, rs8177427, rs8177429, rs6889737, rs3792795, rs8177430, rs8177431, rs4958873, rs8177432, rs8177433, rs8177434, rs8177435, rs3763011, rs8177436, rs8177437, rs4958874, rs8177439, rs8177440, rs8177441, rs8177442, rs8177443, rs869975, rs869976, rs8177444, rs8177445, rs7721469, rs8177446, rs7704191, rs8177447, rs11548, rs2230303, rs7722386, rs8177448, rs8177449, rs2070593, rs8177450, rs8177451, rs8177452, rs8177453, rs8177454, rs3763010, rs8177455, rs8177456, rs736775, rs2277940, rs8177458, rs8177834, rs3924, rs2233312, rs2233311, rs2233310, rs2233309, rs4958875, rs2233308, rs2233307, rs2233306, rs2233305, rs2233304, rs2233303, rs2233302, rs2287719, rs2287720, rs7727034, rs7727250, rs7709800, rs3840312, rs2287721, rs6875293, rs3805434, rs2080982, rs2080983, rs2287722, rs2233301, rs2233300, rs4958876, rs2233299, rs2233298, rs2287723, rs2161359, rs7734456, rs4292439, rs4958878, rs6862024, rs3834819, rs2233297, rs2233296, rs2233295, rs2233294, rs7713028, rs7713223, rs7713567, rs888989, rs2233293, rs3749657, rs2233292, rs2112635, rs871269, rs3792794, rs6579837, rs3805433, rs5872186, rs2233291, rs2233290, rs2233289, rs4958435, rs4958880, rs1422673, rs2042234, rs3805432, rs3805431, rs2233288, rs2233287, rs3815720, rs3792792, rs3792791, rs2303018, rs3792790, rs4958436, rs2233286, rs2233285, rs7732451, rs2233284, rs1422674, rs3792789, rs4562032, rs6865077, rs1559126, rs3792788, rs1559127, rs3792786, rs6880110, rs6861227, rs3805430, rs1862364, rs4958881, rs3792785, rs6869605, rs6870205, rs4246047, rs4958882, rs3792784, rs3792783 and rs5872188.

16. The method of claim 1, wherein the one or more polymorphic variations are detected at one or more positions selected from the group consisting of rs1478398, rs1160114, rs1160113, rs1160112, rs4958858, rs4958431, rs6898463, rs4958859, rs4958860, rs4608909, rs707144, rs2042235, rs3763013, rs2042236, rs8177404, rs8177426, rs8177427, rs8177429, rs3792795, rs4958873, rs8177437, rs869975, rs8177447, rs11548, rs2277940, rs8177834, rs2233311, rs2233302, rs7727034, rs7727250, rs3805434, rs7734456, rs7713028, rs7713223, rs888989, rs3792794, rs4958880, rs1422673, rs3805432 and rs4958436.

17. The method of claim 1, wherein the one or more polymorphic variations are detected in a region spanning positions 231 to 86,539 in SEQ ID NO: 5.

18. The method of claim 1, wherein the one or more polymorphic variations are detected at one or more positions in linkage disequilibrium with one or more positions selected from the grout consisting of rs7500979, rs2217332, rs8044804, rs2270835, rs2133783, rs247609, rs952440, rs881598, rs2291955, rs2518054, rs866038, rs1436425, rs173537, rs247611, rs166017, rs173538, rs193694, rs7205692, rs8048746, rs247618, rs183130, rs6499863, rs4783961, rs3816117, rs711752, rs708272, rs1864163, rs4369653, rs1864165, rs891141, rs891143, rs7205804, rs5885, rs1532625, rs1532624, rs289712, rs7499892, rs5883, rs289714, rs158480, rs289717, rs4344729, rs289718, rs289719, rs2033254, rs4784744, rs291044, rs8053613, rs5881, rs5880, rs7198026, rs5882, rs8045701, rs289741, rs1801706, rs289742, rs289743, rs289746, rs172337, rs289747, rs1566439, rs7205459, rs289749, rs289751, rs8059220, rs8058353, rs289735, rs289737, rs291042, rs1875236, rs821466, rs821465, rs4275846, rs289707, rs821463, rs289706, rs1167741, rs2052880, rs1167742, rs1183256, rs1651665, rs1651666, rs4784751, rs1651667, rs8052091, rs1684574, rs1684575, rs1672865, rs821470, rs1549669, rs291040 and rs289754.

19. The method of claim 1, wherein detecting the presence or absence of the one or more polymorphic variations comprises: hybridizing an oligonucleotide to the nucleic acid sample, wherein the oligonucleotide is complementary to a nucleotide sequence in the nucleic acid and hybridizes to a region adjacent to the polymorphic variation; extending the oligonucleotide in the presence of one or more nucleotides, yielding extension products; and detecting the presence or absence of a polymorphic variation in the extension products.

20. The method of claim 1, wherein the subject is a human.

21-55. (canceled)