Claims
- 1. A method of detecting osteoporosis in a mammalian comprising:
a) obtaining a sample of a bone related tissue or cells; and b) measuring the concentration of at least one marker selected from the group consisting of infectious agents, infectious agent produced factors, and heat shock proteins (HSPs).
- 2. The method of claim 1 further comprising comparing the concentration of a first assay with concentrations of a second or more assays from the same individual over a period of time or against a standard concentration.
- 3. The method of claim 1 wherein the bone related tissue or cells are obtained under conditions that do not induce a HSP response in the mammalian subject.
- 4. The method of claim 3 wherein the HSP is selected from the group consisting of HSP 70, HSP 60, HSP 90, gp 96, cpnlO, cpn20, ubiquitin, and cpn 30.
- 5. The method of claim 2 wherein the time period between the first assay and the second assay is at least about 12 hours.
- 6. The method of claim 1 wherein the sample comprises bone cells or body fluid.
- 7. The method of claim 3 wherein the HSP is HSP 60.
- 8. The method of claim 3 wherein the HSP is HSP 70.
- 9. The method of claim 3 wherein the HSP is ubiquitin.
- 10. The method of claim 3 wherein the concentration of HSP is measured using an immunoassay.
- 11. The method of claim 3 wherein the concentration of HSP is measured using an assay for a nucleotide molecule encoding HSP.
- 12. The method of claim 1 wherein the pathogen is selected from the group consisting of bacteria, viruses, protozoa, parasites and fungi.
- 13. The method of claim 1 wherein the pathogen is selected from the group consisting of bacterial produced factors, viral produced factors, protozoal produced factors, parasitic produced factors and fungal produced factors.
- 14. The method of claim 12 wherein the bacteria is selected from the group consisting of Staphylococcus aureus, Porphyromonas gingivallis, Eikenella corrodens, Actinobacilus actinomycetemcomitans, Prevotella intermedia, Campylobacter rectus, Staphylococcus epidermidis, Salmonella spp., Escherichia coli, Neisseria gonorrhoea, Neisseria meningitis, Mycobacterial tuberculosis, Haemophius influenzae, Pasteurella multocida, B. bronchiseptica, and Fuso bacterium nucleatum.
- 15. The method of claim 1 wherein the pathogen is a bacteria produced factor selected from the group consisting of endotoxin-LPS, gapstatin, and dermonecrotic toxin (DNT).
- 16. The method of claim 15 wherein the factor is selected from the group consisting of gapstatin and dermonecrotic toxin.
- 17. The method of claim 15 wherein the factor is gapstatin.
- 18. The method of claim 15 wherein the factor is dermonecrotic toxin.
- 19. The method of claim 14 wherein the bacteria is selected from the group consisting of Staphylococus aureus, Actinobacillus actinomycetemcomitans, Bordetella bronchiseptica, and Fusobacerium nucleatum.
- 20. A method of treating or preventing osteoporosis caused by an infectious agent, an infectious agent produced factor, or a bone disease comprising administering to a mammalian subject a therapeutically effective amount of a formulation selected from the group consisting of an HSP antigenic formulation and an infectious agent antigenic formulation.
- 21. The method of claim 20 wherein the bone disease is induced by bone infectious agents selected from the group consisting of viruses, bacteria, fungi, protozoa and parasites.
- 22. The method of claim 20 wherein the HSP is complexed with an antigenic material or formulated in combination with an adjuvant.
- 23. The method of claim 20 wherein the antigenic material is a peptide or a protein having an antigenic determinant of a virus, bacteria, fungi, protozoa or parasite that induces a bone disease.
- 24. The method of claim 21 wherein the antigenic material includes an antigenic determinant of a virus selected from the group consisting of immunodeficiency virus type I (HIV-I), human immunodeficiency virus type II (HIV-II), hepatitis type A, hepatitis type B, hepatitis type C, influenza, Varicella, adenovirus, herpes simplex type I (HSV-I), herpes simplex type II (HSV-II), rinderpest, rhinovirus, echovirus, rotavirus, respiratory syncytial virus, papilloma virus, papova virus, cytomegalovirus, echinovirus, arbovirus, huntavirus, coxsackie virus, mumps virus, measles virus, rubella virus and polio virus.
- 25. The method of claim 20 wherein the HSP is selected from the group consisting of HSP 60, HSP 70, HSP 90, gp 96, cpn 10, cpn 20, ubiquitin, cpn 30, and combinations thereof.
- 26. The method of claim 20 wherein the osteoporosis is osteopenia.
- 27. The method of claim 20 wherein the osteoporosis is caused by a bacteria or a bacteria produced factor.
- 28. A kit for use in the method of claim 1.
- 29. A pharmaceutically acceptable composition for administration to a patient for use in the method of claim 20.
FIELD OF THE INVENTION
[0001] This application claims priority to U.S. Ser. No. 60/263,109 entitled “Methods of Using Heat Shock Proteins for Diagnosis and Treatment of Bone Disease” filed Jan. 19, 2001 by Kai-Uwe Lewandrowski and U.S. Ser. No. 60/304,887 entitled “Methods of Diagnosis and Treatment of Osteoporosis” filed Jul. 12, 2001 by Kai-Uwe Lewandrowski. The U.S. Government has rights to this application by virtue of a Grant from the National Institutes of Health.
[0002] The present application generally relates to methods for diagnosing bone loss. More specifically, the present application relates to identifying humoral markers for bone loss on the basis of bacterial or mammalian molecular chaperones.
Provisional Applications (2)
|
Number |
Date |
Country |
|
60263109 |
Jan 2001 |
US |
|
60304887 |
Jul 2001 |
US |