FIELD OF THE INVENTION
The present invention relates to nucleic acids and methods for modulating the sex of avians. In particular, the invention relates to the in ovo delivery of a dsRNA molecule, especially siRNAs, to increase the production of female birds.
BACKGROUND OF THE INVENTION
Man has modified the phenotypic characteristics of domestic animals through selection of seed stock over many generations ever since animals were domesticated. This has led to improvements in quantitative production parameters such as body size and muscle mass. More recent innovations of modifying production traits of poultry and/or improving resistance to pathogens has focussed on transgenic approaches, however, many consumers have concerns about genetically modified organisms.
Chicken producers have been searching for an efficient, economical method of determining the sex of day old chicks. Vent sexing and feather sexing have been used by the various producers, but these methods have been found to have substantial economic disadvantages because of the substantial time required and labour costs in separating the male from the female chicks. The use of probes (U.S. Pat. No. 5,508,165) is also an expensive procedure and not practical economically. Light sensing of anal areas of chicks (U.S. Pat. No. 4,417,663) is another way of determining sex of chicks, but it is also expensive and time consuming as each chick must be handled and manipulated. The use of experts who could feather sex the chicks has been used, but such experts are costly and feathering is time consuming.
There is a need for nucleic acids and methods for modifying avian sex that do not result in transformation of the bird's genome, but are amenable to high throughout processing.
SUMMARY OF THE INVENTION
The present inventors have identified nucleic acid molecules, in particular dsRNA molecules, which can be used to modify the sex of avians in ovo.
Accordingly, in one aspect the present invention provides an isolated and/or exogenous nucleic acid molecule comprising a double-stranded region which reduces the level of at least one RNA molecule and/or protein when administered to an avian egg, wherein if the embryo of the egg is male the sex is altered to female following administration of the isolated and/or exogenous nucleic acid molecule, and wherein the isolated and/or exogenous nucleic acid molecule does not comprise a sequence selected from:
CCAGUUGUCAAGAAGAGCA (SEQ ID NO:254)
GGAUGCUCAUUCAGGACAU (SEQ ID NO:369)
CCCUGUAUCCUUACUAUAA (SEQ ID NO:474)
GCCACUGAGUCUUCCUCAA (SEQ ID NO:530)
CCAGCAACAUACAUGUCAA (SEQ ID NO:605)
CCUGCGUCACACAGAUACU (SEQ ID NO:747)
GGAGUAGUUGUACAGGUUG (SEQ ID NO:3432)
GACUGGCUUGACAUGUAUG (SEQ ID NO:3433)
AUGGCGGUUCUCCAUCCCU (SEQ ID NO:3434) or a variant of any one thereof.
In another aspect, the present invention provides an isolated and/or exogenous nucleic acid molecule comprising one or more of the sequence of nucleotides provided as SEQ ID NO's 11 to 3431 or a variant of any one or more thereof, wherein the isolated and/or exogenous nucleic acid molecule does not comprise a sequence selected from:
CCAGUUGUCAAGAAGAGCA (SEQ ID NO:254)
GGAUGCUCAUUCAGGACAU (SEQ ID NO:369)
CCCUGUAUCCUUACUAUAA (SEQ ID NO:474)
GCCACUGAGUCUUCCUCAA (SEQ ID NO:530)
CCAGCAACAUACAUGUCAA (SEQ ID NO:605)
CCUGCGUCACACAGAUACU (SEQ ID NO:747)
GGAGUAGUUGUACAGGUUG (SEQ ID NO:3432)
GACUGGCUUGACAUGUAUG (SEQ ID NO:3433)
AUGGCGGUUCUCCAUCCCU (SEQ ID NO:3434) or a variant of any one thereof.
In a preferred embodiment, the nucleic acid molecule is dsRNA. More preferably, the dsRNA is a siRNA or a shRNA.
In a preferred embodiment, the nucleic acid molecule reduces the level of a protein encoded by a DMRT1 gene, ASW gene or r-spondin gene, in an avian egg.
In a preferred embodiment of the two above aspects, the nucleic acid does not comprise the full length open reading frame of the RNA molecule or the cDNA encoding therefor. In a related embodiment, preferably the nucleic acid does not comprise a sequence of nucleotides provided as SEQ ID NO's 2, 4 or 6, or a sequence of nucleotides provided as SEQ ID NO's 2, 4 or 6 where each T (thymine) is replaced with a U (uracil).
As the skilled addressee will appreciate, because the nucleic acid is double stranded it will also comprise the corresponding reverse complement of the relevant nucleotide sequence provided herewith.
Also provided is a vector encoding a nucleic acid molecule, or a single strand thereof, according to the invention. Such vectors can be used in a host cell or cell-free expression system to produce nucleic acid molecules useful for the method of the invention.
In another aspect, the present invention provides a host cell comprising an exogenous nucleic acid molecule, or a single strand thereof, of the invention and/or a vector of the invention.
In another aspect, the present invention provides a composition comprising a nucleic acid molecule, or a single strand thereof, of the invention, a vector of the invention, and/or a host cell of the invention.
In a further aspect, the present invention provides a method of modifying the sex of an avian, the method comprising administering to an avian egg at least one nucleic acid molecule of the invention.
Preferably, the nucleic acid is administered to a non-cellular site of the egg. More preferably, the non-cellular site is the air sac, yolk sac, amnionic cavity or chorion allantoic fluid.
In a further preferred embodiment, the egg is not electroporated.
Preferably, the nucleic acid is not delivered by administering a vector encoding the nucleic acid molecule.
Preferably, the nucleic acid molecule administered is dsRNA.
Preferably, the nucleic acid molecule is administered by injection.
Conveniently, the nucleic acid may be administered in a composition of the invention.
In a preferred embodiment, the method modifies the sex of the embryo of the egg from male to female.
The avian can be any species of the Class Ayes. Examples include, but are not limited to, chickens, ducks, turkeys, geese, bantams and quails. In a particularly preferred embodiment, the avian is a chicken.
In a further aspect, the present invention provides an avian produced using a method of the invention.
In another aspect, the present invention provides a chicken produced using a method of the invention.
In a further aspect, the present invention provides an avian egg comprising a nucleic acid molecule, or a single strand thereof, of the invention, a vector of the invention, and/or a host cell of the invention.
In another aspect, the present invention provides a kit comprising a nucleic acid molecule, or a single strand thereof, of the invention, a vector of the invention, a host cell of the invention, and/or a composition of the invention.
As will be apparent, preferred features and characteristics of one aspect of the invention are applicable to many other aspects of the invention.
Throughout this specification the word “comprise”, or variations such as “comprises” or “comprising”, will be understood to imply the inclusion of a stated element, integer or step, or group of elements, integers or steps, but not the exclusion of any other element, integer or step, or group of elements, integers or steps.
The invention is hereinafter described by way of the following non-limiting Examples and with reference to the accompanying figures.
BRIEF DESCRIPTION OF THE ACCOMPANYING DRAWINGS
FIG. 1—Selected shRNAs for knockdown of EGFP-Dmrt1 gene fusion expression. Mean fluorescence intensity for each transfection condition expressed relative to pEGFP-Dmrt1. Error bars indicate standard error calculated on each individual experiment completed in triplicate.
FIG. 2—qPCR analysis of DMRT1 gene expression following silencing.
KEY TO THE SEQUENCE LISTING
SEQ ID NO:1—Partial chicken DMRT1 protein sequence (Genbank AF123456).
SEQ ID NO:2—Partial nucleotide sequence encoding chicken DMRT1 (Genbank AF123456).
SEQ ID NO:3—Chicken WPKCI (ASW) (Genbank AF148455).
SEQ ID NO:4—Nucleotide sequence encoding chicken WPKCI (ASW) (Genbank AF148455).
SEQ ID NO:5—Chicken r-spondin (Genbank XM—417760).
SEQ ID NO:6—Nucleotide sequence encoding chicken r-spondin (Genbank XM—417760).
SEQ ID NO:7—Nucleotide sequence of chicken U6-1 promoter.
SEQ ID NO:8—Nucleotide sequence of chicken U6-3 promoter.
SEQ ID NO:9—Nucleotide sequence of chicken U6-4 promoter.
SEQ ID NO:10—Nucleotide sequence of chicken 7SK promoter.
SEQ ID NO's 11 to 3430—RNA sequences provided in Tables 1 to 3 for silencing the chicken DMRT1, ASW or r-spondin genes.
SEQ ID NO's 3431 to 3434—RNA sequences suitable for silencing the chicken DMRT1 gene.
SEQ ID NO's 3435 to 3485—Target regions of chicken DMRT1.
SEQ ID NO's 3486 to 3499—Oligonucleotide primers and probes.
DETAILED DESCRIPTION OF THE INVENTION
General Techniques and Definitions
Unless specifically defined otherwise, all technical and scientific terms used herein shall be taken to have the same meaning as commonly understood by one of ordinary skill in the art (e.g., in cell culture, molecular genetics, avian biology, RNA interference, and biochemistry).
Unless otherwise indicated, the recombinant protein, cell culture, and immunological techniques utilized in the present invention are standard procedures, well known to those skilled in the art. Such techniques are described and explained throughout the literature in sources such as, J. Perbal, A Practical Guide to Molecular Cloning, John Wiley and Sons (1984), J. Sambrook et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbour Laboratory Press (1989), T. A. Brown (editor), Essential Molecular Biology: A Practical Approach, Volumes 1 and 2, IRL Press (1991), D. M. Glover and B. D. Hames (editors), DNA Cloning: A Practical Approach, Volumes 1-4, IRL Press (1995 and 1996), and F. M. Ausubel et al. (editors), Current Protocols in Molecular Biology, Greene Pub. Associates and Wiley-Interscience (1988, including all updates until present), Ed Harlow and David Lane (editors).
The term “avian” as used herein refers to any species, subspecies or race of organism of the taxonomic Class Ayes, such as, but not limited to, such organisms as chicken, turkey, duck, goose, quail, pheasants, parrots, finches, hawks, crows and ratites including ostrich, emu and cassowary. The term includes the various known strains of Gallus gallus (chickens), for example, White Leghorn, Brown Leghorn, Barred-Rock, Sussex, New Hampshire, Rhode Island, Australorp, Cornish, Minorca, Amrox, California Gray, Italian Partidge-coloured, as well as strains of turkeys, pheasants, quails, duck, ostriches and other poultry commonly bred in commercial quantities.
As used herein, the term “egg” refers to a fertilized ovum that has been laid by a bird. Typically, avian eggs consist of a hard, oval outer eggshell, the “egg white” or albumen, the egg yolk, and various thin membranes. Furthermore, “in ovo” refers to in an egg.
As used herein, the term “non-cellular site” refers a part of the egg other than the embryo.
The terms “reduces”, “reduction” or variations thereof as used herein refers to a measurable decrease in the amount of a target RNA and/or target protein in the egg when compared to an egg from the same species of avian, more preferably strain or breed of avian, and even more preferably the same bird, that has not been administered with a nucleic acid as defined herein. The term also refers to a measurable reduction in the activity of a target protein. Preferably a reduction in the level of a target RNA and/or target protein is at least about 10%. More preferably, the reduction is at least about 20%, 30%, 40%, 50%, 60%, 80%, 90% and even more preferably, about 100%.
As used herein, the phrase “the nucleic acid molecule results in a reduction” or variations thereof refers to the presence of the nucleic acid molecule in the egg inducing degradation of homologous RNAs in the egg by the process known in the art as “RNA interference” or “gene silencing”. Furthermore, the nucleic acid molecule directly results in the reduction, and is not transcribed in ovo to produce the desired effect.
The “at least one RNA molecule” can be any type of RNA present in, and/or produced by, an avian egg. Examples include, but are not limited to, mRNA, snRNA, microRNA and tRNA.
A “variant” of a nucleic acid molecule of the invention includes molecules of varying sizes of, and/or with one or more different nucleotides, but which are still capable of being used to silence the target gene. For example, variants may comprise additional nucleotides (such as 1, 2, 3, 4, or more), or less nucleotides. Furthermore, a few nucleotides may be substituted without influencing the ability of the nucleic acid to silence the target gene. In an embodiment, the variant includes additional 5′ and/or 3′ nucleotides which are homologous to the corresponding target RNA molecule and/or which enhance the stability of the nucleic acid molecule. In another embodiment, the nucleic acid molecules have no more than 4, more preferably no more than 3, more preferably no more than 2, and even more preferably no more than 1, nucleotide differences when compared to the sequences provided herein. In a further embodiment, the nucleic acid molecules have no more than 2, and more preferably no more than 1, internal additional and/or deletional nucleotides when compared to the sequences provided herein. In an embodiment, a nucleic acid of the invention has one, preferably two, additional non-target nucleotides at the 5′ and/or 3′ end, for example an additional UU at the 3′ end. Such additions can increase the half-life of the molecule in ovo.
By an “isolated nucleic acid molecule”, we mean a nucleic acid molecule which is at least partially separated from the nucleic acid molecule with which it is associated or linked in its native state. Preferably, the isolated nucleic acid molecule is at least 60% free, preferably at least 75% free, and most preferably at least 90% free from other components with which they are naturally associated. Furthermore, the term “polynucleotide” is used interchangeably herein with the term “nucleic acid”.
The term “exogenous” in the context of a nucleic acid molecule refers to the nucleic acid molecule when present in a cell, or in a cell-free expression system, in an altered amount. Preferably, the cell is a cell that does not naturally comprise the nucleic acid molecule. However, the cell may be a cell which comprises an exogenous nucleic acid molecule resulting in an increased amount of the nucleic acid molecule. An exogenous nucleic acid molecule of the invention includes nucleic acid molecules which have not been separated from other components of the recombinant cell, or cell-free expression system, in which it is present, and nucleic acid molecules produced in such cells or cell-free systems which are subsequently purified away from at least some other components.
Sex Determination
The present invention relates to the modulation of the sex of avians in ovo. Examples of genes which can be targeted to modulate avian sex include, but are not necessarily limited to, the DMRT1 gene, the ASW (WPKCI) gene, the R-spondin gene, the Fox9 gene and the β-catenin gene.
In a preferred embodiment, the nucleic acid molecule reduces the level of a protein encoded by a DMRT1 gene. DMRT1 was the first molecule implicated in sex determination that shows sequence conservation between phyla. The avian homologue of DMRT1 is found on the Z (sex) chromosome of chickens and is differentially expressed in the genital ridges of male and female chicken embryos (Raymond et al., 1999; Smith et al., 1999). DMRT1 is one of the few genes thus far implicated in mammalian sex determination that appears to have a strictly gonadal pattern of expression (Raymond et al., 1999).
Examples of nucleic acid molecules that can be used to reduce the level of chicken DMRT1 protein, and mRNA encoding therefor, include, but are not limited to, nucleic acids comprising one or more of the sequence of nucleotides provided in Table 1 (SEQ ID NO's 11 to 1644), or a variant of any one or more thereof, with the exception that the nucleic acid molecule does not comprise a sequence selected from:
CCAGUUGUCAAGAAGAGCA (SEQ ID NO:254)
GGAUGCUCAUUCAGGACAU (SEQ ID NO:369)
CCCUGUAUCCUUACUAUAA (SEQ ID NO:474)
GCCACUGAGUCUUCCUCAA (SEQ ID NO:530)
CCAGCAACAUACAUGUCAA (SEQ ID NO:605)
CCUGCGUCACACAGAUACU (SEQ ID NO:747)
GGAGUAGUUGUACAGGUUG (SEQ ID NO:3432) (reverse complement of SEQ ID NO:493)
GACUGGCUUGACAUGUAUG (SEQ ID NO:3433) (reverse complement of SEQ ID NO:612)
AUGGCGGUUCUCCAUCCCU (SEQ ID NO:3434) (reverse complement of SEQ ID NO:1520), or a variant of any one thereof.
In a particularly preferred embodiment, the nucleic acid molecule that can be used to reduce the level of chicken DMRT1 protein comprises a sequence selected from; GAGCCAGUUGUCAAGAAGA (SEQ ID NO:251), GACUGCCAGUGCAAGAAGU (SEQ ID NO:116), CUGUAUCCUUACUAUAACA (SEQ ID NO:476), and CUCCCAGCAACAUACAUGU (SEQ ID NO:602), or a variant of any one thereof. More preferably, the nucleic acid molecule that can be used to reduce the level of chicken DMRT1 protein comprises the sequence, GAGCCAGUUGUCAAGAAGA (SEQ ID NO:251), or a variant thereof such as GAGCCAGUUGUCAAGAAGAUU (SEQ ID NO:3431).
A further example of a gene that can be targeted to modify sex is the WPKCI gene. The avian gene WPKCI has been shown to be conserved widely on the avian W chromosome and expressed actively in the female chicken embryo before the onset of gonadal differentiation. It is suggested that WPKCI may play a role in the differentiation of the female gonad by interfering with the function of PKCI or by exhibiting its unique function in the nucleus (Hori et al., 2000). This gene has also been identified as ASW (avian sex-specific W-linked) (O'Neill et al., 2000).
Examples of nucleic acid molecules that can be used to reduce the level of chicken ASW (WPKCI) protein, and mRNA encoding therefor, include, but are not limited to, nucleic acids comprising one or more of the sequence of nucleotides provided in Table 2 (SEQ ID NO's 1645 to 2209), or a variant of any one or more thereof.
In yet another example of a gene that can be targeted to modify sex is the r-spondin gene. Examples of nucleic acid molecules that can be used to reduce the level of chicken r-spondin protein, and mRNA encoding therefor, include, but are not limited to, nucleic acids comprising one or more of the sequence of nucleotides provided in Table 3 (SEQ ID NO's 2210 to 3430), or a variant of any one or more thereof.
Gene Silencing
The terms “RNA interference”, “RNAi” or “gene silencing” refers generally to a process in which a double-stranded RNA (dsRNA) molecule reduces the expression of a nucleic acid sequence with which the double-stranded RNA molecule shares substantial or total homology. However, it has more recently been shown that gene silencing can be achieved using non-RNA double stranded molecules (see, for example, US 20070004667).
RNA interference (RNAi) is particularly useful for specifically inhibiting the production of a particular RNA and/or protein. Although not wishing to be limited by theory, Waterhouse et al. (1998) have provided a model for the mechanism by which dsRNA (duplex RNA) can be used to reduce protein production. This technology relies on the presence of dsRNA molecules that contain a sequence that is essentially identical to the mRNA of the gene of interest or part thereof, in this case an mRNA encoding a polypeptide of interest. Conveniently, the dsRNA can be produced from a single promoter in a recombinant vector or host cell, where the sense and anti-sense sequences are flanked by an unrelated sequence which enables the sense and anti-sense sequences to hybridize to form the dsRNA molecule with the unrelated sequence forming a loop structure. The design and production of suitable dsRNA molecules for the present invention is well within the capacity of a person skilled in the art, particularly considering Waterhouse et al. (1998), Smith et al. (2000), WO 99/32619, WO 99/53050, WO 99/49029 and WO 01/34815.
The present invention includes nucleic acid molecules comprising and/or encoding double-stranded regions for gene silencing. The nucleic acid molecules are typically RNA but may comprise DNA, chemically-modified nucleotides and non-nucleotides.
TABLE 1
|
|
DsRNA molecules targeting mRNA encoding chicken
|
DMRT1.
|
SEQ ID NO
Sequence 5′-3′
|
|
11
CCGGCGGCGGGCAAGAAGC
|
|
12
CGGCGGCGGGCAAGAAGCU
|
|
13
GGCGGCGGGCAAGAAGCUG
|
|
14
GCGGCGGGCAAGAAGCUGC
|
|
15
CGGCGGGCAAGAAGCUGCC
|
|
16
GGCGGGCAAGAAGCUGCCG
|
|
17
GCGGGCAAGAAGCUGCCGC
|
|
18
CGGGCAAGAAGCUGCCGCG
|
|
19
GGGCAAGAAGCUGCCGCGU
|
|
20
GGCAAGAAGCUGCCGCGUC
|
|
21
GCAAGAAGCUGCCGCGUCU
|
|
22
CAAGAAGCUGCCGCGUCUG
|
|
23
AAGAAGCUGCCGCGUCUGC
|
|
24
AGAAGCUGCCGCGUCUGCC
|
|
25
GAAGCUGCCGCGUCUGCCC
|
|
26
AAGCUGCCGCGUCUGCCCA
|
|
27
AGCUGCCGCGUCUGCCCAA
|
|
28
GCUGCCGCGUCUGCCCAAG
|
|
29
CUGCCGCGUCUGCCCAAGU
|
|
30
UGCCGCGUCUGCCCAAGUG
|
|
31
GCCGCGUCUGCCCAAGUGU
|
|
32
CCGCGUCUGCCCAAGUGUG
|
|
33
CGCGUCUGCCCAAGUGUGC
|
|
34
GCGUCUGCCCAAGUGUGCC
|
|
35
CGUCUGCCCAAGUGUGCCC
|
|
36
GUCUGCCCAAGUGUGCCCG
|
|
37
UCUGCCCAAGUGUGCCCGC
|
|
38
CUGCCCAAGUGUGCCCGCU
|
|
39
UGCCCAAGUGUGCCCGCUG
|
|
40
GCCCAAGUGUGCCCGCUGC
|
|
41
CCCAAGUGUGCCCGCUGCC
|
|
42
CCAAGUGUGCCCGCUGCCG
|
|
43
CAAGUGUGCCCGCUGCCGC
|
|
44
AAGUGUGCCCGCUGCCGCA
|
|
45
AGUGUGCCCGCUGCCGCAA
|
|
46
GUGUGCCCGCUGCCGCAAC
|
|
47
UGUGCCCGCUGCCGCAACC
|
|
48
GUGCCCGCUGCCGCAACCA
|
|
49
UGCCCGCUGCCGCAACCAC
|
|
50
GCCCGCUGCCGCAACCACG
|
|
51
CCCGCUGCCGCAACCACGG
|
|
52
CCGCUGCCGCAACCACGGC
|
|
53
CGCUGCCGCAACCACGGCU
|
|
54
GCUGCCGCAACCACGGCUA
|
|
55
CUGCCGCAACCACGGCUAC
|
|
56
UGCCGCAACCACGGCUACU
|
|
57
GCCGCAACCACGGCUACUC
|
|
58
CCGCAACCACGGCUACUCC
|
|
59
CGCAACCACGGCUACUCCU
|
|
60
GCAACCACGGCUACUCCUC
|
|
61
CAACCACGGCUACUCCUCG
|
|
62
AACCACGGCUACUCCUCGC
|
|
63
ACCACGGCUACUCCUCGCC
|
|
64
CCACGGCUACUCCUCGCCG
|
|
65
CACGGCUACUCCUCGCCGC
|
|
66
ACGGCUACUCCUCGCCGCU
|
|
67
CGGCUACUCCUCGCCGCUG
|
|
68
GGCUACUCCUCGCCGCUGA
|
|
69
GCUACUCCUCGCCGCUGAA
|
|
70
CUACUCCUCGCCGCUGAAG
|
|
71
UACUCCUCGCCGCUGAAGG
|
|
72
ACUCCUCGCCGCUGAAGGG
|
|
73
CUCCUCGCCGCUGAAGGGG
|
|
74
UCCUCGCCGCUGAAGGGGC
|
|
75
CCUCGCCGCUGAAGGGGCA
|
|
76
CUCGCCGCUGAAGGGGCAC
|
|
77
UCGCCGCUGAAGGGGCACA
|
|
78
CGCCGCUGAAGGGGCACAA
|
|
79
GCCGCUGAAGGGGCACAAG
|
|
80
CCGCUGAAGGGGCACAAGC
|
|
81
CGCUGAAGGGGCACAAGCG
|
|
82
GCUGAAGGGGCACAAGCGG
|
|
83
CUGAAGGGGCACAAGCGGU
|
|
84
UGAAGGGGCACAAGCGGUU
|
|
85
GAAGGGGCACAAGCGGUUC
|
|
86
AAGGGGCACAAGCGGUUCU
|
|
87
AGGGGCACAAGCGGUUCUG
|
|
88
GGGGCACAAGCGGUUCUGC
|
|
89
GGGCACAAGCGGUUCUGCA
|
|
90
GGCACAAGCGGUUCUGCAU
|
|
91
GCACAAGCGGUUCUGCAUG
|
|
92
CACAAGCGGUUCUGCAUGU
|
|
93
ACAAGCGGUUCUGCAUGUG
|
|
94
CAAGCGGUUCUGCAUGUGG
|
|
95
AAGCGGUUCUGCAUGUGGC
|
|
96
AGCGGUUCUGCAUGUGGCG
|
|
97
GCGGUUCUGCAUGUGGCGG
|
|
98
CGGUUCUGCAUGUGGCGGG
|
|
99
GGUUCUGCAUGUGGCGGGA
|
|
100
GUUCUGCAUGUGGCGGGAC
|
|
101
UUCUGCAUGUGGCGGGACU
|
|
102
UCUGCAUGUGGCGGGACUG
|
|
103
CUGCAUGUGGCGGGACUGC
|
|
104
UGCAUGUGGCGGGACUGCC
|
|
105
GCAUGUGGCGGGACUGCCA
|
|
106
CAUGUGGCGGGACUGCCAG
|
|
107
AUGUGGCGGGACUGCCAGU
|
|
108
UGUGGCGGGACUGCCAGUG
|
|
109
GUGGCGGGACUGCCAGUGC
|
|
110
UGGCGGGACUGCCAGUGCA
|
|
111
GGCGGGACUGCCAGUGCAA
|
|
112
GCGGGACUGCCAGUGCAAG
|
|
113
CGGGACUGCCAGUGCAAGA
|
|
114
GGGACUGCCAGUGCAAGAA
|
|
115
GGACUGCCAGUGCAAGAAG
|
|
116
GACUGCCAGUGCAAGAAGU
|
|
117
ACUGCCAGUGCAAGAAGUG
|
|
118
CUGCCAGUGCAAGAAGUGC
|
|
119
UGCCAGUGCAAGAAGUGCA
|
|
120
GCCAGUGCAAGAAGUGCAG
|
|
121
CCAGUGCAAGAAGUGCAGC
|
|
122
CAGUGCAAGAAGUGCAGCC
|
|
123
AGUGCAAGAAGUGCAGCCU
|
|
124
GUGCAAGAAGUGCAGCCUG
|
|
125
UGCAAGAAGUGCAGCCUGA
|
|
126
GCAAGAAGUGCAGCCUGAU
|
|
127
CAAGAAGUGCAGCCUGAUC
|
|
128
AAGAAGUGCAGCCUGAUCG
|
|
129
AGAAGUGCAGCCUGAUCGC
|
|
130
GAAGUGCAGCCUGAUCGCC
|
|
131
AAGUGCAGCCUGAUCGCCG
|
|
132
AGUGCAGCCUGAUCGCCGA
|
|
133
GUGCAGCCUGAUCGCCGAG
|
|
134
UGCAGCCUGAUCGCCGAGC
|
|
135
GCAGCCUGAUCGCCGAGCG
|
|
136
CAGCCUGAUCGCCGAGCGG
|
|
137
AGCCUGAUCGCCGAGCGGC
|
|
138
GCCUGAUCGCCGAGCGGCA
|
|
139
CCUGAUCGCCGAGCGGCAG
|
|
140
CUGAUCGCCGAGCGGCAGC
|
|
141
UGAUCGCCGAGCGGCAGCG
|
|
142
GAUCGCCGAGCGGCAGCGG
|
|
143
AUCGCCGAGCGGCAGCGGG
|
|
144
UCGCCGAGCGGCAGCGGGU
|
|
145
CGCCGAGCGGCAGCGGGUG
|
|
146
GCCGAGCGGCAGCGGGUGA
|
|
147
CCGAGCGGCAGCGGGUGAU
|
|
148
CGAGCGGCAGCGGGUGAUG
|
|
149
GAGCGGCAGCGGGUGAUGG
|
|
150
AGCGGCAGCGGGUGAUGGC
|
|
151
GCGGCAGCGGGUGAUGGCC
|
|
152
CGGCAGCGGGUGAUGGCCG
|
|
153
GGCAGCGGGUGAUGGCCGU
|
|
154
GCAGCGGGUGAUGGCCGUG
|
|
155
CAGCGGGUGAUGGCCGUGC
|
|
156
AGCGGGUGAUGGCCGUGCA
|
|
157
GCGGGUGAUGGCCGUGCAG
|
|
158
CGGGUGAUGGCCGUGCAGG
|
|
159
GGGUGAUGGCCGUGCAGGU
|
|
160
GGUGAUGGCCGUGCAGGUU
|
|
161
GUGAUGGCCGUGCAGGUUG
|
|
162
UGAUGGCCGUGCAGGUUGC
|
|
163
GAUGGCCGUGCAGGUUGCA
|
|
164
AUGGCCGUGCAGGUUGCAC
|
|
165
UGGCCGUGCAGGUUGCACU
|
|
166
GGCCGUGCAGGUUGCACUG
|
|
167
GCCGUGCAGGUUGCACUGA
|
|
168
CCGUGCAGGUUGCACUGAG
|
|
169
CGUGCAGGUUGCACUGAGG
|
|
170
GUGCAGGUUGCACUGAGGA
|
|
171
UGCAGGUUGCACUGAGGAG
|
|
172
GCAGGUUGCACUGAGGAGG
|
|
173
CAGGUUGCACUGAGGAGGC
|
|
174
AGGUUGCACUGAGGAGGCA
|
|
175
GGUUGCACUGAGGAGGCAG
|
|
176
GUUGCACUGAGGAGGCAGC
|
|
177
UUGCACUGAGGAGGCAGCA
|
|
178
UGCACUGAGGAGGCAGCAA
|
|
179
GCACUGAGGAGGCAGCAAG
|
|
180
CACUGAGGAGGCAGCAAGC
|
|
181
ACUGAGGAGGCAGCAAGCC
|
|
182
CUGAGGAGGCAGCAAGCCC
|
|
183
UGAGGAGGCAGCAAGCCCA
|
|
184
GAGGAGGCAGCAAGCCCAG
|
|
185
AGGAGGCAGCAAGCCCAGG
|
|
186
GGAGGCAGCAAGCCCAGGA
|
|
187
GAGGCAGCAAGCCCAGGAA
|
|
188
AGGCAGCAAGCCCAGGAAG
|
|
189
GGCAGCAAGCCCAGGAAGA
|
|
190
GCAGCAAGCCCAGGAAGAG
|
|
191
CAGCAAGCCCAGGAAGAGG
|
|
192
AGCAAGCCCAGGAAGAGGA
|
|
193
GCAAGCCCAGGAAGAGGAG
|
|
194
CAAGCCCAGGAAGAGGAGC
|
|
195
AAGCCCAGGAAGAGGAGCU
|
|
196
AGCCCAGGAAGAGGAGCUG
|
|
197
GCCCAGGAAGAGGAGCUGG
|
|
198
CCCAGGAAGAGGAGCUGGG
|
|
199
CCAGGAAGAGGAGCUGGGG
|
|
200
CAGGAAGAGGAGCUGGGGA
|
|
201
AGGAAGAGGAGCUGGGGAU
|
|
202
GGAAGAGGAGCUGGGGAUC
|
|
203
GAAGAGGAGCUGGGGAUCA
|
|
204
AAGAGGAGCUGGGGAUCAG
|
|
205
AGAGGAGCUGGGGAUCAGC
|
|
206
GAGGAGCUGGGGAUCAGCC
|
|
207
AGGAGCUGGGGAUCAGCCA
|
|
208
GGAGCUGGGGAUCAGCCAC
|
|
209
GAGCUGGGGAUCAGCCACC
|
|
210
AGCUGGGGAUCAGCCACCC
|
|
211
GCUGGGGAUCAGCCACCCU
|
|
212
CUGGGGAUCAGCCACCCUG
|
|
213
UGGGGAUCAGCCACCCUGU
|
|
214
GGGGAUCAGCCACCCUGUA
|
|
215
GGGAUCAGCCACCCUGUAC
|
|
216
GGAUCAGCCACCCUGUACC
|
|
217
GAUCAGCCACCCUGUACCC
|
|
218
AUCAGCCACCCUGUACCCC
|
|
219
UCAGCCACCCUGUACCCCU
|
|
220
CAGCCACCCUGUACCCCUG
|
|
221
AGCCACCCUGUACCCCUGC
|
|
222
GCCACCCUGUACCCCUGCC
|
|
223
CCACCCUGUACCCCUGCCC
|
|
224
CACCCUGUACCCCUGCCCA
|
|
225
ACCCUGUACCCCUGCCCAG
|
|
226
CCCUGUACCCCUGCCCAGU
|
|
227
CCUGUACCCCUGCCCAGUG
|
|
228
CUGUACCCCUGCCCAGUGC
|
|
229
UGUACCCCUGCCCAGUGCC
|
|
230
GUACCCCUGCCCAGUGCCC
|
|
231
UACCCCUGCCCAGUGCCCC
|
|
232
ACCCCUGCCCAGUGCCCCU
|
|
233
CCCCUGCCCAGUGCCCCUG
|
|
234
CCCUGCCCAGUGCCCCUGA
|
|
235
CCUGCCCAGUGCCCCUGAG
|
|
236
CUGCCCAGUGCCCCUGAGC
|
|
237
UGCCCAGUGCCCCUGAGCC
|
|
238
GCCCAGUGCCCCUGAGCCA
|
|
239
CCCAGUGCCCCUGAGCCAG
|
|
240
CCAGUGCCCCUGAGCCAGU
|
|
241
CAGUGCCCCUGAGCCAGUU
|
|
242
AGUGCCCCUGAGCCAGUUG
|
|
243
GUGCCCCUGAGCCAGUUGU
|
|
244
UGCCCCUGAGCCAGUUGUC
|
|
245
GCCCCUGAGCCAGUUGUCA
|
|
246
CCCCUGAGCCAGUUGUCAA
|
|
247
CCCUGAGCCAGUUGUCAAG
|
|
248
CCUGAGCCAGUUGUCAAGA
|
|
249
CUGAGCCAGUUGUCAAGAA
|
|
250
UGAGCCAGUUGUCAAGAAG
|
|
251
GAGCCAGUUGUCAAGAAGA
|
|
252
AGCCAGUUGUCAAGAAGAG
|
|
253
GCCAGUUGUCAAGAAGAGC
|
|
254
CCAGUUGUCAAGAAGAGCA
|
|
255
CAGUUGUCAAGAAGAGCAG
|
|
256
AGUUGUCAAGAAGAGCAGC
|
|
257
GUUGUCAAGAAGAGCAGCA
|
|
258
UUGUCAAGAAGAGCAGCAG
|
|
259
UGUCAAGAAGAGCAGCAGC
|
|
260
GUCAAGAAGAGCAGCAGCA
|
|
261
UCAAGAAGAGCAGCAGCAG
|
|
262
CAAGAAGAGCAGCAGCAGC
|
|
263
AAGAAGAGCAGCAGCAGCA
|
|
264
AGAAGAGCAGCAGCAGCAG
|
|
265
GAAGAGCAGCAGCAGCAGC
|
|
266
AAGAGCAGCAGCAGCAGCU
|
|
267
AGAGCAGCAGCAGCAGCUC
|
|
268
GAGCAGCAGCAGCAGCUCC
|
|
269
AGCAGCAGCAGCAGCUCCU
|
|
270
GCAGCAGCAGCAGCUCCUG
|
|
271
CAGCAGCAGCAGCUCCUGU
|
|
272
AGCAGCAGCAGCUCCUGUC
|
|
273
GCAGCAGCAGCUCCUGUCU
|
|
274
CAGCAGCAGCUCCUGUCUC
|
|
275
AGCAGCAGCUCCUGUCUCC
|
|
276
GCAGCAGCUCCUGUCUCCU
|
|
277
CAGCAGCUCCUGUCUCCUG
|
|
278
AGCAGCUCCUGUCUCCUGC
|
|
279
GCAGCUCCUGUCUCCUGCA
|
|
280
CAGCUCCUGUCUCCUGCAG
|
|
281
AGCUCCUGUCUCCUGCAGG
|
|
282
GCUCCUGUCUCCUGCAGGA
|
|
283
CUCCUGUCUCCUGCAGGAC
|
|
284
UCCUGUCUCCUGCAGGACA
|
|
285
CCUGUCUCCUGCAGGACAG
|
|
286
CUGUCUCCUGCAGGACAGC
|
|
287
UGUCUCCUGCAGGACAGCA
|
|
288
GUCUCCUGCAGGACAGCAG
|
|
289
UCUCCUGCAGGACAGCAGC
|
|
290
CUCCUGCAGGACAGCAGCA
|
|
291
UCCUGCAGGACAGCAGCAG
|
|
292
CCUGCAGGACAGCAGCAGC
|
|
293
CUGCAGGACAGCAGCAGCC
|
|
294
UGCAGGACAGCAGCAGCCC
|
|
295
GCAGGACAGCAGCAGCCCU
|
|
296
CAGGACAGCAGCAGCCCUG
|
|
297
AGGACAGCAGCAGCCCUGC
|
|
298
GGACAGCAGCAGCCCUGCU
|
|
299
GACAGCAGCAGCCCUGCUC
|
|
300
ACAGCAGCAGCCCUGCUCA
|
|
301
CAGCAGCAGCCCUGCUCAC
|
|
302
AGCAGCAGCCCUGCUCACU
|
|
303
GCAGCAGCCCUGCUCACUC
|
|
304
CAGCAGCCCUGCUCACUCC
|
|
305
AGCAGCCCUGCUCACUCCA
|
|
306
GCAGCCCUGCUCACUCCAC
|
|
307
CAGCCCUGCUCACUCCACG
|
|
308
AGCCCUGCUCACUCCACGA
|
|
309
GCCCUGCUCACUCCACGAG
|
|
310
CCCUGCUCACUCCACGAGC
|
|
311
CCUGCUCACUCCACGAGCA
|
|
312
CUGCUCACUCCACGAGCAC
|
|
313
UGCUCACUCCACGAGCACG
|
|
314
GCUCACUCCACGAGCACGG
|
|
315
CUCACUCCACGAGCACGGU
|
|
316
UCACUCCACGAGCACGGUG
|
|
317
CACUCCACGAGCACGGUGG
|
|
318
ACUCCACGAGCACGGUGGC
|
|
319
CUCCACGAGCACGGUGGCA
|
|
320
UCCACGAGCACGGUGGCAG
|
|
321
CCACGAGCACGGUGGCAGC
|
|
322
CACGAGCACGGUGGCAGCA
|
|
323
ACGAGCACGGUGGCAGCAG
|
|
324
CGAGCACGGUGGCAGCAGC
|
|
325
GAGCACGGUGGCAGCAGCA
|
|
326
AGCACGGUGGCAGCAGCAG
|
|
327
GCACGGUGGCAGCAGCAGC
|
|
328
CACGGUGGCAGCAGCAGCA
|
|
329
ACGGUGGCAGCAGCAGCAG
|
|
330
CGGUGGCAGCAGCAGCAGC
|
|
331
GGUGGCAGCAGCAGCAGCG
|
|
332
GUGGCAGCAGCAGCAGCGA
|
|
333
UGGCAGCAGCAGCAGCGAG
|
|
334
GGCAGCAGCAGCAGCGAGC
|
|
335
GCAGCAGCAGCAGCGAGCG
|
|
336
CAGCAGCAGCAGCGAGCGC
|
|
337
AGCAGCAGCAGCGAGCGCA
|
|
338
GCAGCAGCAGCGAGCGCAC
|
|
339
CAGCAGCAGCGAGCGCACC
|
|
340
AGCAGCAGCGAGCGCACCA
|
|
341
GCAGCAGCGAGCGCACCAC
|
|
342
CAGCAGCGAGCGCACCACC
|
|
343
AGCAGCGAGCGCACCACCA
|
|
344
GCAGCGAGCGCACCACCAG
|
|
345
CAGCGAGCGCACCACCAGA
|
|
346
AGCGAGCGCACCACCAGAG
|
|
347
GCGAGCGCACCACCAGAGG
|
|
348
CGAGCGCACCACCAGAGGG
|
|
349
GAGCGCACCACCAGAGGGA
|
|
350
AGCGCACCACCAGAGGGAC
|
|
351
GCGCACCACCAGAGGGACG
|
|
352
CGCACCACCAGAGGGACGG
|
|
353
GCACCACCAGAGGGACGGA
|
|
354
CACCACCAGAGGGACGGAU
|
|
355
ACCACCAGAGGGACGGAUG
|
|
356
CCACCAGAGGGACGGAUGC
|
|
357
CACCAGAGGGACGGAUGCU
|
|
358
ACCAGAGGGACGGAUGCUC
|
|
359
CCAGAGGGACGGAUGCUCA
|
|
360
CAGAGGGACGGAUGCUCAU
|
|
361
AGAGGGACGGAUGCUCAUU
|
|
362
GAGGGACGGAUGCUCAUUC
|
|
363
AGGGACGGAUGCUCAUUCA
|
|
364
GGGACGGAUGCUCAUUCAG
|
|
365
GGACGGAUGCUCAUUCAGG
|
|
366
GACGGAUGCUCAUUCAGGA
|
|
367
ACGGAUGCUCAUUCAGGAC
|
|
368
CGGAUGCUCAUUCAGGACA
|
|
369
GGAUGCUCAUUCAGGACAU
|
|
370
GAUGCUCAUUCAGGACAUC
|
|
371
AUGCUCAUUCAGGACAUCC
|
|
372
UGCUCAUUCAGGACAUCCC
|
|
373
GCUCAUUCAGGACAUCCCU
|
|
374
CUCAUUCAGGACAUCCCUU
|
|
375
UCAUUCAGGACAUCCCUUC
|
|
376
CAUUCAGGACAUCCCUUCC
|
|
377
AUUCAGGACAUCCCUUCCA
|
|
378
UUCAGGACAUCCCUUCCAU
|
|
379
UCAGGACAUCCCUUCCAUC
|
|
380
CAGGACAUCCCUUCCAUCC
|
|
381
AGGACAUCCCUUCCAUCCC
|
|
382
GGACAUCCCUUCCAUCCCC
|
|
383
GACAUCCCUUCCAUCCCCA
|
|
384
ACAUCCCUUCCAUCCCCAG
|
|
385
CAUCCCUUCCAUCCCCAGC
|
|
386
AUCCCUUCCAUCCCCAGCA
|
|
387
UCCCUUCCAUCCCCAGCAG
|
|
388
CCCUUCCAUCCCCAGCAGA
|
|
389
CCUUCCAUCCCCAGCAGAG
|
|
390
CUUCCAUCCCCAGCAGAGG
|
|
391
UUCCAUCCCCAGCAGAGGG
|
|
392
UCCAUCCCCAGCAGAGGGC
|
|
393
CCAUCCCCAGCAGAGGGCA
|
|
394
CAUCCCCAGCAGAGGGCAC
|
|
395
AUCCCCAGCAGAGGGCACU
|
|
396
UCCCCAGCAGAGGGCACUU
|
|
397
CCCCAGCAGAGGGCACUUG
|
|
398
CCCAGCAGAGGGCACUUGG
|
|
399
CCAGCAGAGGGCACUUGGA
|
|
400
CAGCAGAGGGCACUUGGAG
|
|
401
AGCAGAGGGCACUUGGAGA
|
|
402
GCAGAGGGCACUUGGAGAG
|
|
403
CAGAGGGCACUUGGAGAGC
|
|
404
AGAGGGCACUUGGAGAGCA
|
|
405
GAGGGCACUUGGAGAGCAC
|
|
406
AGGGCACUUGGAGAGCACG
|
|
407
GGGCACUUGGAGAGCACGU
|
|
408
GGCACUUGGAGAGCACGUC
|
|
409
GCACUUGGAGAGCACGUCU
|
|
410
CACUUGGAGAGCACGUCUG
|
|
411
ACUUGGAGAGCACGUCUGA
|
|
412
CUUGGAGAGCACGUCUGAU
|
|
413
UUGGAGAGCACGUCUGAUU
|
|
414
UGGAGAGCACGUCUGAUUU
|
|
415
GGAGAGCACGUCUGAUUUG
|
|
416
GAGAGCACGUCUGAUUUGG
|
|
417
AGAGCACGUCUGAUUUGGU
|
|
418
GAGCACGUCUGAUUUGGUU
|
|
419
AGCACGUCUGAUUUGGUUG
|
|
420
GCACGUCUGAUUUGGUUGU
|
|
421
CACGUCUGAUUUGGUUGUG
|
|
422
ACGUCUGAUUUGGUUGUGG
|
|
423
CGUCUGAUUUGGUUGUGGA
|
|
424
GUCUGAUUUGGUUGUGGAC
|
|
425
UCUGAUUUGGUUGUGGACU
|
|
426
CUGAUUUGGUUGUGGACUC
|
|
427
UGAUUUGGUUGUGGACUCC
|
|
428
GAUUUGGUUGUGGACUCCA
|
|
429
AUUUGGUUGUGGACUCCAC
|
|
430
UUUGGUUGUGGACUCCACC
|
|
431
UUGGUUGUGGACUCCACCU
|
|
432
UGGUUGUGGACUCCACCUA
|
|
433
GGUUGUGGACUCCACCUAC
|
|
434
GUUGUGGACUCCACCUACU
|
|
435
UUGUGGACUCCACCUACUA
|
|
436
UGUGGACUCCACCUACUAC
|
|
437
GUGGACUCCACCUACUACA
|
|
438
UGGACUCCACCUACUACAG
|
|
439
GGACUCCACCUACUACAGC
|
|
440
GACUCCACCUACUACAGCA
|
|
441
ACUCCACCUACUACAGCAG
|
|
442
CUCCACCUACUACAGCAGU
|
|
443
UCCACCUACUACAGCAGUU
|
|
444
CCACCUACUACAGCAGUUU
|
|
445
CACCUACUACAGCAGUUUU
|
|
446
ACCUACUACAGCAGUUUUU
|
|
447
CCUACUACAGCAGUUUUUA
|
|
448
CUACUACAGCAGUUUUUAC
|
|
449
UACUACAGCAGUUUUUACC
|
|
450
ACUACAGCAGUUUUUACCA
|
|
451
CUACAGCAGUUUUUACCAG
|
|
452
UACAGCAGUUUUUACCAGC
|
|
453
ACAGCAGUUUUUACCAGCC
|
|
454
CAGCAGUUUUUACCAGCCA
|
|
455
AGCAGUUUUUACCAGCCAU
|
|
456
GCAGUUUUUACCAGCCAUC
|
|
457
CAGUUUUUACCAGCCAUCC
|
|
458
AGUUUUUACCAGCCAUCCC
|
|
459
GUUUUUACCAGCCAUCCCU
|
|
460
UUUUUACCAGCCAUCCCUG
|
|
461
UUUUACCAGCCAUCCCUGU
|
|
462
UUUACCAGCCAUCCCUGUA
|
|
463
UUACCAGCCAUCCCUGUAU
|
|
464
UACCAGCCAUCCCUGUAUC
|
|
465
ACCAGCCAUCCCUGUAUCC
|
|
466
CCAGCCAUCCCUGUAUCCU
|
|
467
CAGCCAUCCCUGUAUCCUU
|
|
468
AGCCAUCCCUGUAUCCUUA
|
|
469
GCCAUCCCUGUAUCCUUAC
|
|
470
CCAUCCCUGUAUCCUUACU
|
|
471
CAUCCCUGUAUCCUUACUA
|
|
472
AUCCCUGUAUCCUUACUAU
|
|
473
UCCCUGUAUCCUUACUAUA
|
|
474
CCCUGUAUCCUUACUAUAA
|
|
475
CCUGUAUCCUUACUAUAAC
|
|
476
CUGUAUCCUUACUAUAACA
|
|
477
UGUAUCCUUACUAUAACAA
|
|
478
GUAUCCUUACUAUAACAAC
|
|
479
UAUCCUUACUAUAACAACC
|
|
480
AUCCUUACUAUAACAACCU
|
|
481
UCCUUACUAUAACAACCUG
|
|
482
CCUUACUAUAACAACCUGU
|
|
483
CUUACUAUAACAACCUGUA
|
|
484
UUACUAUAACAACCUGUAC
|
|
485
UACUAUAACAACCUGUACA
|
|
486
ACUAUAACAACCUGUACAA
|
|
487
CUAUAACAACCUGUACAAC
|
|
488
UAUAACAACCUGUACAACU
|
|
489
AUAACAACCUGUACAACUA
|
|
490
UAACAACCUGUACAACUAC
|
|
491
AACAACCUGUACAACUACU
|
|
492
ACAACCUGUACAACUACUC
|
|
493
CAACCUGUACAACUACUCC
|
|
494
AACCUGUACAACUACUCCC
|
|
495
ACCUGUACAACUACUCCCA
|
|
496
CCUGUACAACUACUCCCAG
|
|
497
CUGUACAACUACUCCCAGU
|
|
498
UGUACAACUACUCCCAGUA
|
|
499
GUACAACUACUCCCAGUAC
|
|
500
UACAACUACUCCCAGUACC
|
|
501
ACAACUACUCCCAGUACCA
|
|
502
CAACUACUCCCAGUACCAA
|
|
503
AACUACUCCCAGUACCAAA
|
|
504
ACUACUCCCAGUACCAAAU
|
|
505
CUACUCCCAGUACCAAAUG
|
|
506
UACUCCCAGUACCAAAUGG
|
|
507
ACUCCCAGUACCAAAUGGC
|
|
508
CUCCCAGUACCAAAUGGCA
|
|
509
UCCCAGUACCAAAUGGCAG
|
|
510
CCCAGUACCAAAUGGCAGU
|
|
511
CCAGUACCAAAUGGCAGUG
|
|
512
CAGUACCAAAUGGCAGUGG
|
|
513
AGUACCAAAUGGCAGUGGC
|
|
514
GUACCAAAUGGCAGUGGCC
|
|
515
UACCAAAUGGCAGUGGCCA
|
|
516
ACCAAAUGGCAGUGGCCAC
|
|
517
CCAAAUGGCAGUGGCCACU
|
|
518
CAAAUGGCAGUGGCCACUG
|
|
519
AAAUGGCAGUGGCCACUGA
|
|
520
AAUGGCAGUGGCCACUGAG
|
|
521
AUGGCAGUGGCCACUGAGU
|
|
522
UGGCAGUGGCCACUGAGUC
|
|
523
GGCAGUGGCCACUGAGUCU
|
|
524
GCAGUGGCCACUGAGUCUU
|
|
525
CAGUGGCCACUGAGUCUUC
|
|
526
AGUGGCCACUGAGUCUUCC
|
|
527
GUGGCCACUGAGUCUUCCU
|
|
528
UGGCCACUGAGUCUUCCUC
|
|
529
GGCCACUGAGUCUUCCUCA
|
|
530
GCCACUGAGUCUUCCUCAA
|
|
531
CCACUGAGUCUUCCUCAAG
|
|
532
CACUGAGUCUUCCUCAAGU
|
|
533
ACUGAGUCUUCCUCAAGUG
|
|
534
CUGAGUCUUCCUCAAGUGA
|
|
535
UGAGUCUUCCUCAAGUGAG
|
|
536
GAGUCUUCCUCAAGUGAGA
|
|
537
AGUCUUCCUCAAGUGAGAC
|
|
538
GUCUUCCUCAAGUGAGACA
|
|
539
UCUUCCUCAAGUGAGACAG
|
|
540
CUUCCUCAAGUGAGACAGG
|
|
541
UUCCUCAAGUGAGACAGGG
|
|
542
UCCUCAAGUGAGACAGGGG
|
|
543
CCUCAAGUGAGACAGGGGG
|
|
544
CUCAAGUGAGACAGGGGGU
|
|
545
UCAAGUGAGACAGGGGGUA
|
|
546
CAAGUGAGACAGGGGGUAC
|
|
547
AAGUGAGACAGGGGGUACG
|
|
548
AGUGAGACAGGGGGUACGU
|
|
549
GUGAGACAGGGGGUACGUU
|
|
550
UGAGACAGGGGGUACGUUU
|
|
551
GAGACAGGGGGUACGUUUG
|
|
552
AGACAGGGGGUACGUUUGU
|
|
553
GACAGGGGGUACGUUUGUA
|
|
554
ACAGGGGGUACGUUUGUAG
|
|
555
CAGGGGGUACGUUUGUAGG
|
|
556
AGGGGGUACGUUUGUAGGG
|
|
557
GGGGGUACGUUUGUAGGGU
|
|
558
GGGGUACGUUUGUAGGGUC
|
|
559
GGGUACGUUUGUAGGGUCA
|
|
560
GGUACGUUUGUAGGGUCAG
|
|
561
GUACGUUUGUAGGGUCAGC
|
|
562
UACGUUUGUAGGGUCAGCC
|
|
563
ACGUUUGUAGGGUCAGCCA
|
|
564
CGUUUGUAGGGUCAGCCAU
|
|
565
GUUUGUAGGGUCAGCCAUG
|
|
566
UUUGUAGGGUCAGCCAUGA
|
|
567
UUGUAGGGUCAGCCAUGAA
|
|
568
UGUAGGGUCAGCCAUGAAA
|
|
569
GUAGGGUCAGCCAUGAAAA
|
|
570
UAGGGUCAGCCAUGAAAAA
|
|
571
AGGGUCAGCCAUGAAAAAC
|
|
572
GGGUCAGCCAUGAAAAACA
|
|
573
GGUCAGCCAUGAAAAACAG
|
|
574
GUCAGCCAUGAAAAACAGC
|
|
575
UCAGCCAUGAAAAACAGCC
|
|
576
CAGCCAUGAAAAACAGCCU
|
|
577
AGCCAUGAAAAACAGCCUU
|
|
578
GCCAUGAAAAACAGCCUUC
|
|
579
CCAUGAAAAACAGCCUUCG
|
|
580
CAUGAAAAACAGCCUUCGA
|
|
581
AUGAAAAACAGCCUUCGAA
|
|
582
UGAAAAACAGCCUUCGAAG
|
|
583
GAAAAACAGCCUUCGAAGC
|
|
584
AAAAACAGCCUUCGAAGCC
|
|
585
AAAACAGCCUUCGAAGCCU
|
|
586
AAACAGCCUUCGAAGCCUC
|
|
587
AACAGCCUUCGAAGCCUCC
|
|
588
ACAGCCUUCGAAGCCUCCC
|
|
589
CAGCCUUCGAAGCCUCCCA
|
|
590
AGCCUUCGAAGCCUCCCAG
|
|
591
GCCUUCGAAGCCUCCCAGC
|
|
592
CCUUCGAAGCCUCCCAGCA
|
|
593
CUUCGAAGCCUCCCAGCAA
|
|
594
UUCGAAGCCUCCCAGCAAC
|
|
595
UCGAAGCCUCCCAGCAACA
|
|
596
CGAAGCCUCCCAGCAACAU
|
|
597
GAAGCCUCCCAGCAACAUA
|
|
598
AAGCCUCCCAGCAACAUAC
|
|
599
AGCCUCCCAGCAACAUACA
|
|
600
GCCUCCCAGCAACAUACAU
|
|
601
CCUCCCAGCAACAUACAUG
|
|
602
CUCCCAGCAACAUACAUGU
|
|
603
UCCCAGCAACAUACAUGUC
|
|
604
CCCAGCAACAUACAUGUCA
|
|
605
CCAGCAACAUACAUGUCAA
|
|
606
CAGCAACAUACAUGUCAAG
|
|
607
AGCAACAUACAUGUCAAGC
|
|
608
GCAACAUACAUGUCAAGCC
|
|
609
CAACAUACAUGUCAAGCCA
|
|
610
AACAUACAUGUCAAGCCAG
|
|
611
ACAUACAUGUCAAGCCAGU
|
|
612
CAUACAUGUCAAGCCAGUC
|
|
613
AUACAUGUCAAGCCAGUCA
|
|
614
UACAUGUCAAGCCAGUCAG
|
|
615
ACAUGUCAAGCCAGUCAGG
|
|
616
CAUGUCAAGCCAGUCAGGA
|
|
617
AUGUCAAGCCAGUCAGGAA
|
|
618
UGUCAAGCCAGUCAGGAAA
|
|
619
GUCAAGCCAGUCAGGAAAA
|
|
620
UCAAGCCAGUCAGGAAAAC
|
|
621
CAAGCCAGUCAGGAAAACA
|
|
622
AAGCCAGUCAGGAAAACAG
|
|
623
AGCCAGUCAGGAAAACAGU
|
|
624
GCCAGUCAGGAAAACAGUG
|
|
625
CCAGUCAGGAAAACAGUGG
|
|
626
CAGUCAGGAAAACAGUGGC
|
|
627
AGUCAGGAAAACAGUGGCA
|
|
628
GUCAGGAAAACAGUGGCAG
|
|
629
UCAGGAAAACAGUGGCAGA
|
|
630
CAGGAAAACAGUGGCAGAU
|
|
631
AGGAAAACAGUGGCAGAUG
|
|
632
GGAAAACAGUGGCAGAUGA
|
|
633
GAAAACAGUGGCAGAUGAA
|
|
634
AAAACAGUGGCAGAUGAAG
|
|
635
AAACAGUGGCAGAUGAAGG
|
|
636
AACAGUGGCAGAUGAAGGG
|
|
637
ACAGUGGCAGAUGAAGGGA
|
|
638
CAGUGGCAGAUGAAGGGAA
|
|
639
AGUGGCAGAUGAAGGGAAU
|
|
640
GUGGCAGAUGAAGGGAAUG
|
|
641
UGGCAGAUGAAGGGAAUGG
|
|
642
GGCAGAUGAAGGGAAUGGA
|
|
643
GCAGAUGAAGGGAAUGGAG
|
|
644
CAGAUGAAGGGAAUGGAGA
|
|
645
AGAUGAAGGGAAUGGAGAA
|
|
646
GAUGAAGGGAAUGGAGAAC
|
|
647
AUGAAGGGAAUGGAGAACC
|
|
648
UGAAGGGAAUGGAGAACCG
|
|
649
GAAGGGAAUGGAGAACCGC
|
|
650
AAGGGAAUGGAGAACCGCC
|
|
651
AGGGAAUGGAGAACCGCCA
|
|
652
GGGAAUGGAGAACCGCCAU
|
|
653
GGAAUGGAGAACCGCCAUG
|
|
654
GAAUGGAGAACCGCCAUGC
|
|
655
AAUGGAGAACCGCCAUGCC
|
|
656
AUGGAGAACCGCCAUGCCA
|
|
657
UGGAGAACCGCCAUGCCAU
|
|
658
GGAGAACCGCCAUGCCAUG
|
|
659
GAGAACCGCCAUGCCAUGA
|
|
660
AGAACCGCCAUGCCAUGAG
|
|
661
GAACCGCCAUGCCAUGAGC
|
|
662
AACCGCCAUGCCAUGAGCU
|
|
663
ACCGCCAUGCCAUGAGCUC
|
|
664
CCGCCAUGCCAUGAGCUCC
|
|
665
CGCCAUGCCAUGAGCUCCC
|
|
666
GCCAUGCCAUGAGCUCCCA
|
|
667
CCAUGCCAUGAGCUCCCAG
|
|
668
CAUGCCAUGAGCUCCCAGU
|
|
669
AUGCCAUGAGCUCCCAGUA
|
|
670
UGCCAUGAGCUCCCAGUAC
|
|
671
GCCAUGAGCUCCCAGUACC
|
|
672
CCAUGAGCUCCCAGUACCG
|
|
673
CAUGAGCUCCCAGUACCGG
|
|
674
AUGAGCUCCCAGUACCGGA
|
|
675
UGAGCUCCCAGUACCGGAU
|
|
676
GAGCUCCCAGUACCGGAUG
|
|
677
AGCUCCCAGUACCGGAUGU
|
|
678
GCUCCCAGUACCGGAUGUG
|
|
679
CUCCCAGUACCGGAUGUGC
|
|
680
UCCCAGUACCGGAUGUGCU
|
|
681
CCCAGUACCGGAUGUGCUC
|
|
682
CCAGUACCGGAUGUGCUCC
|
|
683
CAGUACCGGAUGUGCUCCU
|
|
684
AGUACCGGAUGUGCUCCUA
|
|
685
GUACCGGAUGUGCUCCUAC
|
|
686
UACCGGAUGUGCUCCUACU
|
|
687
ACCGGAUGUGCUCCUACUA
|
|
688
CCGGAUGUGCUCCUACUAC
|
|
689
CGGAUGUGCUCCUACUACC
|
|
690
GGAUGUGCUCCUACUACCC
|
|
691
GAUGUGCUCCUACUACCCG
|
|
692
AUGUGCUCCUACUACCCGC
|
|
693
UGUGCUCCUACUACCCGCC
|
|
694
GUGCUCCUACUACCCGCCC
|
|
695
UGCUCCUACUACCCGCCCA
|
|
696
GCUCCUACUACCCGCCCAC
|
|
697
CUCCUACUACCCGCCCACC
|
|
698
UCCUACUACCCGCCCACCU
|
|
699
CCUACUACCCGCCCACCUC
|
|
700
CUACUACCCGCCCACCUCA
|
|
701
UACUACCCGCCCACCUCAU
|
|
702
ACUACCCGCCCACCUCAUA
|
|
703
CUACCCGCCCACCUCAUAC
|
|
704
UACCCGCCCACCUCAUACC
|
|
705
ACCCGCCCACCUCAUACCU
|
|
706
CCCGCCCACCUCAUACCUG
|
|
707
CCGCCCACCUCAUACCUGG
|
|
708
CGCCCACCUCAUACCUGGG
|
|
709
GCCCACCUCAUACCUGGGC
|
|
710
CCCACCUCAUACCUGGGCC
|
|
711
CCACCUCAUACCUGGGCCA
|
|
712
CACCUCAUACCUGGGCCAG
|
|
713
ACCUCAUACCUGGGCCAGG
|
|
714
CCUCAUACCUGGGCCAGGG
|
|
715
CUCAUACCUGGGCCAGGGG
|
|
716
UCAUACCUGGGCCAGGGGG
|
|
717
CAUACCUGGGCCAGGGGGU
|
|
718
AUACCUGGGCCAGGGGGUU
|
|
719
UACCUGGGCCAGGGGGUUG
|
|
720
ACCUGGGCCAGGGGGUUGG
|
|
721
CCUGGGCCAGGGGGUUGGC
|
|
722
CUGGGCCAGGGGGUUGGCA
|
|
723
UGGGCCAGGGGGUUGGCAG
|
|
724
GGGCCAGGGGGUUGGCAGU
|
|
725
GGCCAGGGGGUUGGCAGUC
|
|
726
GCCAGGGGGUUGGCAGUCC
|
|
727
CCAGGGGGUUGGCAGUCCC
|
|
728
CAGGGGGUUGGCAGUCCCA
|
|
729
AGGGGGUUGGCAGUCCCAC
|
|
730
GGGGGUUGGCAGUCCCACC
|
|
731
GGGGUUGGCAGUCCCACCU
|
|
732
GGGUUGGCAGUCCCACCUG
|
|
733
GGUUGGCAGUCCCACCUGC
|
|
734
GUUGGCAGUCCCACCUGCG
|
|
735
UUGGCAGUCCCACCUGCGU
|
|
736
UGGCAGUCCCACCUGCGUC
|
|
737
GGCAGUCCCACCUGCGUCA
|
|
738
GCAGUCCCACCUGCGUCAC
|
|
739
CAGUCCCACCUGCGUCACA
|
|
740
AGUCCCACCUGCGUCACAC
|
|
741
GUCCCACCUGCGUCACACA
|
|
742
UCCCACCUGCGUCACACAG
|
|
743
CCCACCUGCGUCACACAGA
|
|
744
CCACCUGCGUCACACAGAU
|
|
745
CACCUGCGUCACACAGAUA
|
|
746
ACCUGCGUCACACAGAUAC
|
|
747
CCUGCGUCACACAGAUACU
|
|
748
CUGCGUCACACAGAUACUG
|
|
749
UGCGUCACACAGAUACUGG
|
|
750
GCGUCACACAGAUACUGGC
|
|
751
CGUCACACAGAUACUGGCC
|
|
752
GUCACACAGAUACUGGCCU
|
|
753
UCACACAGAUACUGGCCUC
|
|
754
CACACAGAUACUGGCCUCG
|
|
755
ACACAGAUACUGGCCUCGG
|
|
756
CACAGAUACUGGCCUCGGA
|
|
757
ACAGAUACUGGCCUCGGAG
|
|
758
CAGAUACUGGCCUCGGAGG
|
|
759
AGAUACUGGCCUCGGAGGA
|
|
760
GAUACUGGCCUCGGAGGAC
|
|
761
AUACUGGCCUCGGAGGACA
|
|
762
UACUGGCCUCGGAGGACAC
|
|
763
ACUGGCCUCGGAGGACACC
|
|
764
CUGGCCUCGGAGGACACCC
|
|
765
UGGCCUCGGAGGACACCCC
|
|
766
GGCCUCGGAGGACACCCCC
|
|
767
GCCUCGGAGGACACCCCCU
|
|
768
CCUCGGAGGACACCCCCUC
|
|
769
CUCGGAGGACACCCCCUCC
|
|
770
UCGGAGGACACCCCCUCCU
|
|
771
CGGAGGACACCCCCUCCUA
|
|
772
GGAGGACACCCCCUCCUAC
|
|
773
GAGGACACCCCCUCCUACU
|
|
774
AGGACACCCCCUCCUACUC
|
|
775
GGACACCCCCUCCUACUCA
|
|
776
GACACCCCCUCCUACUCAG
|
|
777
ACACCCCCUCCUACUCAGA
|
|
778
CACCCCCUCCUACUCAGAG
|
|
779
ACCCCCUCCUACUCAGAGU
|
|
780
CCCCCUCCUACUCAGAGUC
|
|
781
CCCCUCCUACUCAGAGUCG
|
|
782
CCCUCCUACUCAGAGUCGA
|
|
783
CCUCCUACUCAGAGUCGAA
|
|
784
CUCCUACUCAGAGUCGAAA
|
|
785
UCCUACUCAGAGUCGAAAG
|
|
786
CCUACUCAGAGUCGAAAGC
|
|
787
CUACUCAGAGUCGAAAGCG
|
|
788
UACUCAGAGUCGAAAGCGA
|
|
789
ACUCAGAGUCGAAAGCGAG
|
|
790
CUCAGAGUCGAAAGCGAGA
|
|
791
UCAGAGUCGAAAGCGAGAG
|
|
792
CAGAGUCGAAAGCGAGAGU
|
|
793
AGAGUCGAAAGCGAGAGUG
|
|
794
GAGUCGAAAGCGAGAGUGU
|
|
795
AGUCGAAAGCGAGAGUGUU
|
|
796
GUCGAAAGCGAGAGUGUUU
|
|
797
UCGAAAGCGAGAGUGUUUU
|
|
798
CGAAAGCGAGAGUGUUUUC
|
|
799
GAAAGCGAGAGUGUUUUCG
|
|
800
AAAGCGAGAGUGUUUUCGC
|
|
801
AAGCGAGAGUGUUUUCGCC
|
|
802
AGCGAGAGUGUUUUCGCCG
|
|
803
GCGAGAGUGUUUUCGCCGC
|
|
804
CGAGAGUGUUUUCGCCGCC
|
|
805
GAGAGUGUUUUCGCCGCCC
|
|
806
AGAGUGUUUUCGCCGCCCA
|
|
807
GAGUGUUUUCGCCGCCCAG
|
|
808
AGUGUUUUCGCCGCCCAGC
|
|
809
GUGUUUUCGCCGCCCAGCA
|
|
810
UGUUUUCGCCGCCCAGCAG
|
|
811
GUUUUCGCCGCCCAGCAGC
|
|
812
UUUUCGCCGCCCAGCAGCC
|
|
813
UUUCGCCGCCCAGCAGCCA
|
|
814
UUCGCCGCCCAGCAGCCAG
|
|
815
UCGCCGCCCAGCAGCCAGG
|
|
816
CGCCGCCCAGCAGCCAGGA
|
|
817
GCCGCCCAGCAGCCAGGAC
|
|
818
CCGCCCAGCAGCCAGGACU
|
|
819
CGCCCAGCAGCCAGGACUC
|
|
820
GCCCAGCAGCCAGGACUCG
|
|
821
CCCAGCAGCCAGGACUCGG
|
|
822
CCAGCAGCCAGGACUCGGG
|
|
823
CAGCAGCCAGGACUCGGGC
|
|
824
AGCAGCCAGGACUCGGGCC
|
|
825
GCAGCCAGGACUCGGGCCU
|
|
826
CAGCCAGGACUCGGGCCUG
|
|
827
AGCCAGGACUCGGGCCUGG
|
|
828
GCCAGGACUCGGGCCUGGG
|
|
829
CCAGGACUCGGGCCUGGGG
|
|
830
CAGGACUCGGGCCUGGGGU
|
|
831
AGGACUCGGGCCUGGGGUG
|
|
832
GGACUCGGGCCUGGGGUGC
|
|
833
GACUCGGGCCUGGGGUGCC
|
|
834
ACUCGGGCCUGGGGUGCCU
|
|
835
CUCGGGCCUGGGGUGCCUG
|
|
836
UCGGGCCUGGGGUGCCUGU
|
|
837
CGGGCCUGGGGUGCCUGUC
|
|
838
GGGCCUGGGGUGCCUGUCG
|
|
839
GGCCUGGGGUGCCUGUCGA
|
|
840
GCCUGGGGUGCCUGUCGAG
|
|
841
CCUGGGGUGCCUGUCGAGC
|
|
842
CUGGGGUGCCUGUCGAGCA
|
|
843
UGGGGUGCCUGUCGAGCAG
|
|
844
GGGGUGCCUGUCGAGCAGC
|
|
845
GGGUGCCUGUCGAGCAGCG
|
|
846
GGUGCCUGUCGAGCAGCGA
|
|
847
GUGCCUGUCGAGCAGCGAG
|
|
848
UGCCUGUCGAGCAGCGAGA
|
|
849
GCCUGUCGAGCAGCGAGAG
|
|
850
CCUGUCGAGCAGCGAGAGC
|
|
851
CUGUCGAGCAGCGAGAGCA
|
|
852
UGUCGAGCAGCGAGAGCAC
|
|
853
GUCGAGCAGCGAGAGCACC
|
|
854
UCGAGCAGCGAGAGCACCA
|
|
855
CGAGCAGCGAGAGCACCAA
|
|
856
GAGCAGCGAGAGCACCAAG
|
|
857
AGCAGCGAGAGCACCAAGG
|
|
858
GCAGCGAGAGCACCAAGGG
|
|
859
CAGCGAGAGCACCAAGGGA
|
|
860
AGCGAGAGCACCAAGGGAG
|
|
861
GCGAGAGCACCAAGGGAGA
|
|
862
CGAGAGCACCAAGGGAGAC
|
|
863
GAGAGCACCAAGGGAGACC
|
|
864
AGAGCACCAAGGGAGACCU
|
|
865
GAGCACCAAGGGAGACCUG
|
|
866
AGCACCAAGGGAGACCUGG
|
|
867
GCACCAAGGGAGACCUGGA
|
|
868
CACCAAGGGAGACCUGGAG
|
|
869
ACCAAGGGAGACCUGGAGU
|
|
870
CCAAGGGAGACCUGGAGUG
|
|
871
CAAGGGAGACCUGGAGUGC
|
|
872
AAGGGAGACCUGGAGUGCG
|
|
873
AGGGAGACCUGGAGUGCGA
|
|
874
GGGAGACCUGGAGUGCGAG
|
|
875
GGAGACCUGGAGUGCGAGC
|
|
876
GAGACCUGGAGUGCGAGCC
|
|
877
AGACCUGGAGUGCGAGCCC
|
|
878
GACCUGGAGUGCGAGCCCC
|
|
879
ACCUGGAGUGCGAGCCCCA
|
|
880
CCUGGAGUGCGAGCCCCAC
|
|
881
CUGGAGUGCGAGCCCCACC
|
|
882
UGGAGUGCGAGCCCCACCA
|
|
883
GGAGUGCGAGCCCCACCAA
|
|
884
GAGUGCGAGCCCCACCAAG
|
|
885
AGUGCGAGCCCCACCAAGA
|
|
886
GUGCGAGCCCCACCAAGAG
|
|
887
UGCGAGCCCCACCAAGAGC
|
|
888
GCGAGCCCCACCAAGAGCC
|
|
889
CGAGCCCCACCAAGAGCCC
|
|
890
GAGCCCCACCAAGAGCCCG
|
|
891
AGCCCCACCAAGAGCCCGG
|
|
892
GCCCCACCAAGAGCCCGGC
|
|
893
CCCCACCAAGAGCCCGGCG
|
|
894
CCCACCAAGAGCCCGGCGC
|
|
895
CCACCAAGAGCCCGGCGCC
|
|
896
CACCAAGAGCCCGGCGCCU
|
|
897
ACCAAGAGCCCGGCGCCUU
|
|
898
CCAAGAGCCCGGCGCCUUC
|
|
899
CAAGAGCCCGGCGCCUUCG
|
|
900
AAGAGCCCGGCGCCUUCGC
|
|
901
AGAGCCCGGCGCCUUCGCG
|
|
902
GAGCCCGGCGCCUUCGCGG
|
|
903
AGCCCGGCGCCUUCGCGGU
|
|
904
GCCCGGCGCCUUCGCGGUG
|
|
905
CCCGGCGCCUUCGCGGUGA
|
|
906
CCGGCGCCUUCGCGGUGAG
|
|
907
CGGCGCCUUCGCGGUGAGC
|
|
908
GGCGCCUUCGCGGUGAGCC
|
|
909
GCGCCUUCGCGGUGAGCCC
|
|
910
CGCCUUCGCGGUGAGCCCG
|
|
911
GCCUUCGCGGUGAGCCCGG
|
|
912
CCUUCGCGGUGAGCCCGGU
|
|
913
CUUCGCGGUGAGCCCGGUU
|
|
914
UUCGCGGUGAGCCCGGUUC
|
|
915
UCGCGGUGAGCCCGGUUCU
|
|
916
CGCGGUGAGCCCGGUUCUU
|
|
917
GCGGUGAGCCCGGUUCUUG
|
|
918
CGGUGAGCCCGGUUCUUGA
|
|
919
GGUGAGCCCGGUUCUUGAG
|
|
920
GUGAGCCCGGUUCUUGAGG
|
|
921
UGAGCCCGGUUCUUGAGGG
|
|
922
GAGCCCGGUUCUUGAGGGC
|
|
923
AGCCCGGUUCUUGAGGGCG
|
|
924
GCCCGGUUCUUGAGGGCGA
|
|
925
CCCGGUUCUUGAGGGCGAG
|
|
926
CCGGUUCUUGAGGGCGAGU
|
|
927
CGGUUCUUGAGGGCGAGUA
|
|
928
GGUUCUUGAGGGCGAGUAG
|
|
929
GUUCUUGAGGGCGAGUAGG
|
|
930
UUCUUGAGGGCGAGUAGGC
|
|
931
UCUUGAGGGCGAGUAGGCG
|
|
932
CUUGAGGGCGAGUAGGCGC
|
|
933
UUGAGGGCGAGUAGGCGCG
|
|
934
UGAGGGCGAGUAGGCGCGG
|
|
935
GAGGGCGAGUAGGCGCGGC
|
|
936
AGGGCGAGUAGGCGCGGCG
|
|
937
GGGCGAGUAGGCGCGGCGU
|
|
938
GGCGAGUAGGCGCGGCGUC
|
|
939
GCGAGUAGGCGCGGCGUCG
|
|
940
CGAGUAGGCGCGGCGUCGG
|
|
941
GAGUAGGCGCGGCGUCGGG
|
|
942
AGUAGGCGCGGCGUCGGGC
|
|
943
GUAGGCGCGGCGUCGGGCG
|
|
944
UAGGCGCGGCGUCGGGCGG
|
|
945
AGGCGCGGCGUCGGGCGGC
|
|
946
GGCGCGGCGUCGGGCGGCU
|
|
947
GCGCGGCGUCGGGCGGCUG
|
|
948
CGCGGCGUCGGGCGGCUGC
|
|
949
GCGGCGUCGGGCGGCUGCU
|
|
950
CGGCGUCGGGCGGCUGCUG
|
|
951
GGCGUCGGGCGGCUGCUGC
|
|
952
GCGUCGGGCGGCUGCUGCG
|
|
953
CGUCGGGCGGCUGCUGCGC
|
|
954
GUCGGGCGGCUGCUGCGCG
|
|
955
UCGGGCGGCUGCUGCGCGG
|
|
956
CGGGCGGCUGCUGCGCGGC
|
|
957
GGGCGGCUGCUGCGCGGCG
|
|
958
GGCGGCUGCUGCGCGGCGU
|
|
959
GCGGCUGCUGCGCGGCGUU
|
|
960
CGGCUGCUGCGCGGCGUUC
|
|
961
GGCUGCUGCGCGGCGUUCA
|
|
962
GCUGCUGCGCGGCGUUCAC
|
|
963
CUGCUGCGCGGCGUUCACU
|
|
964
UGCUGCGCGGCGUUCACUG
|
|
965
GCUGCGCGGCGUUCACUGU
|
|
966
CUGCGCGGCGUUCACUGUU
|
|
967
UGCGCGGCGUUCACUGUUG
|
|
968
GCGCGGCGUUCACUGUUGC
|
|
969
CGCGGCGUUCACUGUUGCC
|
|
970
GCGGCGUUCACUGUUGCCU
|
|
971
CGGCGUUCACUGUUGCCUU
|
|
972
GGCGUUCACUGUUGCCUUG
|
|
973
GCGUUCACUGUUGCCUUGU
|
|
974
CGUUCACUGUUGCCUUGUU
|
|
975
GUUCACUGUUGCCUUGUUC
|
|
976
UUCACUGUUGCCUUGUUCU
|
|
977
UCACUGUUGCCUUGUUCUG
|
|
978
CACUGUUGCCUUGUUCUGU
|
|
979
ACUGUUGCCUUGUUCUGUU
|
|
980
CUGUUGCCUUGUUCUGUUG
|
|
981
UGUUGCCUUGUUCUGUUGG
|
|
982
GUUGCCUUGUUCUGUUGGG
|
|
983
UUGCCUUGUUCUGUUGGGG
|
|
984
UGCCUUGUUCUGUUGGGGU
|
|
985
GCCUUGUUCUGUUGGGGUU
|
|
986
CCUUGUUCUGUUGGGGUUG
|
|
987
CUUGUUCUGUUGGGGUUGC
|
|
988
UUGUUCUGUUGGGGUUGCG
|
|
989
UGUUCUGUUGGGGUUGCGG
|
|
990
GUUCUGUUGGGGUUGCGGG
|
|
991
UUCUGUUGGGGUUGCGGGG
|
|
992
UCUGUUGGGGUUGCGGGGG
|
|
993
CUGUUGGGGUUGCGGGGGG
|
|
994
UGUUGGGGUUGCGGGGGGG
|
|
995
GUUGGGGUUGCGGGGGGGC
|
|
996
UUGGGGUUGCGGGGGGGCG
|
|
997
UGGGGUUGCGGGGGGGCGU
|
|
998
GGGGUUGCGGGGGGGCGUU
|
|
999
GGGUUGCGGGGGGGCGUUG
|
|
1000
GGUUGCGGGGGGGCGUUGG
|
|
1001
GUUGCGGGGGGGCGUUGGG
|
|
1002
UUGCGGGGGGGCGUUGGGU
|
|
1003
UGCGGGGGGGCGUUGGGUU
|
|
1004
GCGGGGGGGCGUUGGGUUU
|
|
1005
CGGGGGGGCGUUGGGUUUC
|
|
1006
GGGGGGGCGUUGGGUUUCU
|
|
1007
GGGGGGCGUUGGGUUUCUU
|
|
1008
GGGGGCGUUGGGUUUCUUC
|
|
1009
GGGGCGUUGGGUUUCUUCU
|
|
1010
GGGCGUUGGGUUUCUUCUU
|
|
1011
GGCGUUGGGUUUCUUCUUU
|
|
1012
GCGUUGGGUUUCUUCUUUC
|
|
1013
CGUUGGGUUUCUUCUUUCC
|
|
1014
GUUGGGUUUCUUCUUUCCG
|
|
1015
UUGGGUUUCUUCUUUCCGG
|
|
1016
UGGGUUUCUUCUUUCCGGG
|
|
1017
GGGUUUCUUCUUUCCGGGG
|
|
1018
GGUUUCUUCUUUCCGGGGC
|
|
1019
GUUUCUUCUUUCCGGGGCG
|
|
1020
UUUCUUCUUUCCGGGGCGG
|
|
1021
UUCUUCUUUCCGGGGCGGG
|
|
1022
UCUUCUUUCCGGGGCGGGG
|
|
1023
CUUCUUUCCGGGGCGGGGG
|
|
1024
UUCUUUCCGGGGCGGGGGG
|
|
1025
UCUUUCCGGGGCGGGGGGG
|
|
1026
CUUUCCGGGGCGGGGGGGG
|
|
1027
UUUCCGGGGCGGGGGGGGC
|
|
1028
UUCCGGGGCGGGGGGGGCA
|
|
1029
UCCGGGGCGGGGGGGGCAC
|
|
1030
CCGGGGCGGGGGGGGCACG
|
|
1031
CGGGGCGGGGGGGGCACGG
|
|
1032
GGGGCGGGGGGGGCACGGC
|
|
1033
GGGCGGGGGGGGCACGGCG
|
|
1034
GGCGGGGGGGGCACGGCGG
|
|
1035
GCGGGGGGGGCACGGCGGG
|
|
1036
CGGGGGGGGCACGGCGGGG
|
|
1037
GGGGGGGGCACGGCGGGGC
|
|
1038
GGGGGGGCACGGCGGGGCC
|
|
1039
GGGGGGCACGGCGGGGCCG
|
|
1040
GGGGGCACGGCGGGGCCGC
|
|
1041
GGGGCACGGCGGGGCCGCG
|
|
1042
GGGCACGGCGGGGCCGCGG
|
|
1043
GGCACGGCGGGGCCGCGGC
|
|
1044
GCACGGCGGGGCCGCGGCC
|
|
1045
CACGGCGGGGCCGCGGCCG
|
|
1046
ACGGCGGGGCCGCGGCCGG
|
|
1047
CGGCGGGGCCGCGGCCGGG
|
|
1048
GGCGGGGCCGCGGCCGGGC
|
|
1049
GCGGGGCCGCGGCCGGGCC
|
|
1050
CGGGGCCGCGGCCGGGCCG
|
|
1051
GGGGCCGCGGCCGGGCCGG
|
|
1052
GGGCCGCGGCCGGGCCGGC
|
|
1053
GGCCGCGGCCGGGCCGGCG
|
|
1054
GCCGCGGCCGGGCCGGCGG
|
|
1055
CCGCGGCCGGGCCGGCGGG
|
|
1056
CGCGGCCGGGCCGGCGGGG
|
|
1057
GCGGCCGGGCCGGCGGGGC
|
|
1058
CGGCCGGGCCGGCGGGGCG
|
|
1059
GGCCGGGCCGGCGGGGCGG
|
|
1060
GCCGGGCCGGCGGGGCGGG
|
|
1061
CCGGGCCGGCGGGGCGGGG
|
|
1062
CGGGCCGGCGGGGCGGGGC
|
|
1063
GGGCCGGCGGGGCGGGGCG
|
|
1064
GGCCGGCGGGGCGGGGCGG
|
|
1065
GCCGGCGGGGCGGGGCGGG
|
|
1066
CCGGCGGGGCGGGGCGGGG
|
|
1067
CGGCGGGGCGGGGCGGGGC
|
|
1068
GGCGGGGCGGGGCGGGGCG
|
|
1069
GCGGGGCGGGGCGGGGCGG
|
|
1070
CGGGGCGGGGCGGGGCGGG
|
|
1071
GGGGCGGGGCGGGGCGGGA
|
|
1072
GGGCGGGGCGGGGCGGGAC
|
|
1073
GGCGGGGCGGGGCGGGACG
|
|
1074
GCGGGGCGGGGCGGGACGG
|
|
1075
CGGGGCGGGGCGGGACGGG
|
|
1076
GGGGCGGGGCGGGACGGGG
|
|
1077
GGGCGGGGCGGGACGGGGC
|
|
1078
GGCGGGGCGGGACGGGGCG
|
|
1079
GCGGGGCGGGACGGGGCGG
|
|
1080
CGGGGCGGGACGGGGCGGG
|
|
1081
GGGGCGGGACGGGGCGGGG
|
|
1082
GGGCGGGACGGGGCGGGGC
|
|
1083
GGCGGGACGGGGCGGGGCG
|
|
1084
GCGGGACGGGGCGGGGCGG
|
|
1085
CGGGACGGGGCGGGGCGGA
|
|
1086
GGGACGGGGCGGGGCGGAG
|
|
1087
GGACGGGGCGGGGCGGAGC
|
|
1088
GACGGGGCGGGGCGGAGCC
|
|
1089
ACGGGGCGGGGCGGAGCCG
|
|
1090
CGGGGCGGGGCGGAGCCGC
|
|
1091
GGGGCGGGGCGGAGCCGCG
|
|
1092
GGGCGGGGCGGAGCCGCGC
|
|
1093
GGCGGGGCGGAGCCGCGCG
|
|
1094
GCGGGGCGGAGCCGCGCGG
|
|
1095
CGGGGCGGAGCCGCGCGGG
|
|
1096
GGGGCGGAGCCGCGCGGGG
|
|
1097
GGGCGGAGCCGCGCGGGGG
|
|
1098
GGCGGAGCCGCGCGGGGGC
|
|
1099
GCGGAGCCGCGCGGGGGCC
|
|
1100
CGGAGCCGCGCGGGGGCCG
|
|
1101
GGAGCCGCGCGGGGGCCGC
|
|
1102
GAGCCGCGCGGGGGCCGCA
|
|
1103
AGCCGCGCGGGGGCCGCAG
|
|
1104
GCCGCGCGGGGGCCGCAGU
|
|
1105
CCGCGCGGGGGCCGCAGUC
|
|
1106
CGCGCGGGGGCCGCAGUCC
|
|
1107
GCGCGGGGGCCGCAGUCCG
|
|
1108
CGCGGGGGCCGCAGUCCGG
|
|
1109
GCGGGGGCCGCAGUCCGGG
|
|
1110
CGGGGGCCGCAGUCCGGGC
|
|
1111
GGGGGCCGCAGUCCGGGCC
|
|
1112
GGGGCCGCAGUCCGGGCCG
|
|
1113
GGGCCGCAGUCCGGGCCGG
|
|
1114
GGCCGCAGUCCGGGCCGGG
|
|
1115
GCCGCAGUCCGGGCCGGGG
|
|
1116
CCGCAGUCCGGGCCGGGGC
|
|
1117
CGCAGUCCGGGCCGGGGCC
|
|
1118
GCAGUCCGGGCCGGGGCCG
|
|
1119
CAGUCCGGGCCGGGGCCGC
|
|
1120
AGUCCGGGCCGGGGCCGCC
|
|
1121
GUCCGGGCCGGGGCCGCCG
|
|
1122
UCCGGGCCGGGGCCGCCGU
|
|
1123
CCGGGCCGGGGCCGCCGUC
|
|
1124
CGGGCCGGGGCCGCCGUCG
|
|
1125
GGGCCGGGGCCGCCGUCGG
|
|
1126
GGCCGGGGCCGCCGUCGGG
|
|
1127
GCCGGGGCCGCCGUCGGGU
|
|
1128
CCGGGGCCGCCGUCGGGUC
|
|
1129
CGGGGCCGCCGUCGGGUCU
|
|
1130
GGGGCCGCCGUCGGGUCUC
|
|
1131
GGGCCGCCGUCGGGUCUCG
|
|
1132
GGCCGCCGUCGGGUCUCGG
|
|
1133
GCCGCCGUCGGGUCUCGGC
|
|
1134
CCGCCGUCGGGUCUCGGCC
|
|
1135
CGCCGUCGGGUCUCGGCCC
|
|
1136
GCCGUCGGGUCUCGGCCCG
|
|
1137
CCGUCGGGUCUCGGCCCGC
|
|
1138
CGUCGGGUCUCGGCCCGCU
|
|
1139
GUCGGGUCUCGGCCCGCUC
|
|
1140
UCGGGUCUCGGCCCGCUCC
|
|
1141
CGGGUCUCGGCCCGCUCCC
|
|
1142
GGGUCUCGGCCCGCUCCCG
|
|
1143
GGUCUCGGCCCGCUCCCGU
|
|
1144
GUCUCGGCCCGCUCCCGUC
|
|
1145
UCUCGGCCCGCUCCCGUCG
|
|
1146
CUCGGCCCGCUCCCGUCGG
|
|
1147
UCGGCCCGCUCCCGUCGGG
|
|
1148
CGGCCCGCUCCCGUCGGGG
|
|
1149
GGCCCGCUCCCGUCGGGGC
|
|
1150
GCCCGCUCCCGUCGGGGCG
|
|
1151
CCCGCUCCCGUCGGGGCGG
|
|
1152
CCGCUCCCGUCGGGGCGGA
|
|
1153
CGCUCCCGUCGGGGCGGAG
|
|
1154
GCUCCCGUCGGGGCGGAGC
|
|
1155
CUCCCGUCGGGGCGGAGCG
|
|
1156
UCCCGUCGGGGCGGAGCGU
|
|
1157
CCCGUCGGGGCGGAGCGUC
|
|
1158
CCGUCGGGGCGGAGCGUCC
|
|
1159
CGUCGGGGCGGAGCGUCCG
|
|
1160
GUCGGGGCGGAGCGUCCGA
|
|
1161
UCGGGGCGGAGCGUCCGAC
|
|
1162
CGGGGCGGAGCGUCCGACG
|
|
1163
GGGGCGGAGCGUCCGACGA
|
|
1164
GGGCGGAGCGUCCGACGAU
|
|
1165
GGCGGAGCGUCCGACGAUC
|
|
1166
GCGGAGCGUCCGACGAUCG
|
|
1167
CGGAGCGUCCGACGAUCGG
|
|
1168
GGAGCGUCCGACGAUCGGC
|
|
1169
GAGCGUCCGACGAUCGGCC
|
|
1170
AGCGUCCGACGAUCGGCCU
|
|
1171
GCGUCCGACGAUCGGCCUC
|
|
1172
CGUCCGACGAUCGGCCUCC
|
|
1173
GUCCGACGAUCGGCCUCCA
|
|
1174
UCCGACGAUCGGCCUCCAC
|
|
1175
CCGACGAUCGGCCUCCACG
|
|
1176
CGACGAUCGGCCUCCACGA
|
|
1177
GACGAUCGGCCUCCACGAA
|
|
1178
ACGAUCGGCCUCCACGAAA
|
|
1179
CGAUCGGCCUCCACGAAAC
|
|
1180
GAUCGGCCUCCACGAAACG
|
|
1181
AUCGGCCUCCACGAAACGC
|
|
1182
UCGGCCUCCACGAAACGCG
|
|
1183
CGGCCUCCACGAAACGCGG
|
|
1184
GGCCUCCACGAAACGCGGU
|
|
1185
GCCUCCACGAAACGCGGUG
|
|
1186
CCUCCACGAAACGCGGUGC
|
|
1187
CUCCACGAAACGCGGUGCC
|
|
1188
UCCACGAAACGCGGUGCCG
|
|
1189
CCACGAAACGCGGUGCCGU
|
|
1190
CACGAAACGCGGUGCCGUG
|
|
1191
ACGAAACGCGGUGCCGUGA
|
|
1192
CGAAACGCGGUGCCGUGAU
|
|
1193
GAAACGCGGUGCCGUGAUG
|
|
1194
AAACGCGGUGCCGUGAUGU
|
|
1195
AACGCGGUGCCGUGAUGUG
|
|
1196
ACGCGGUGCCGUGAUGUGU
|
|
1197
CGCGGUGCCGUGAUGUGUU
|
|
1198
GCGGUGCCGUGAUGUGUUU
|
|
1199
CGGUGCCGUGAUGUGUUUG
|
|
1200
GGUGCCGUGAUGUGUUUGU
|
|
1201
GUGCCGUGAUGUGUUUGUA
|
|
1202
UGCCGUGAUGUGUUUGUAG
|
|
1203
GCCGUGAUGUGUUUGUAGU
|
|
1204
CCGUGAUGUGUUUGUAGUG
|
|
1205
CGUGAUGUGUUUGUAGUGG
|
|
1206
GUGAUGUGUUUGUAGUGGU
|
|
1207
UGAUGUGUUUGUAGUGGUU
|
|
1208
GAUGUGUUUGUAGUGGUUC
|
|
1209
AUGUGUUUGUAGUGGUUCC
|
|
1210
UGUGUUUGUAGUGGUUCCU
|
|
1211
GUGUUUGUAGUGGUUCCUC
|
|
1212
UGUUUGUAGUGGUUCCUCG
|
|
1213
GUUUGUAGUGGUUCCUCGU
|
|
1214
UUUGUAGUGGUUCCUCGUA
|
|
1215
UUGUAGUGGUUCCUCGUAG
|
|
1216
UGUAGUGGUUCCUCGUAGG
|
|
1217
GUAGUGGUUCCUCGUAGGC
|
|
1218
UAGUGGUUCCUCGUAGGCU
|
|
1219
AGUGGUUCCUCGUAGGCUC
|
|
1220
GUGGUUCCUCGUAGGCUCC
|
|
1221
UGGUUCCUCGUAGGCUCCA
|
|
1222
GGUUCCUCGUAGGCUCCAG
|
|
1223
GUUCCUCGUAGGCUCCAGA
|
|
1224
UUCCUCGUAGGCUCCAGAC
|
|
1225
UCCUCGUAGGCUCCAGACG
|
|
1226
CCUCGUAGGCUCCAGACGU
|
|
1227
CUCGUAGGCUCCAGACGUU
|
|
1228
UCGUAGGCUCCAGACGUUU
|
|
1229
CGUAGGCUCCAGACGUUUU
|
|
1230
GUAGGCUCCAGACGUUUUC
|
|
1231
UAGGCUCCAGACGUUUUCU
|
|
1232
AGGCUCCAGACGUUUUCUC
|
|
1233
GGCUCCAGACGUUUUCUCC
|
|
1234
GCUCCAGACGUUUUCUCCU
|
|
1235
CUCCAGACGUUUUCUCCUC
|
|
1236
UCCAGACGUUUUCUCCUCG
|
|
1237
CCAGACGUUUUCUCCUCGU
|
|
1238
CAGACGUUUUCUCCUCGUA
|
|
1239
AGACGUUUUCUCCUCGUAU
|
|
1240
GACGUUUUCUCCUCGUAUC
|
|
1241
ACGUUUUCUCCUCGUAUCG
|
|
1242
CGUUUUCUCCUCGUAUCGC
|
|
1243
GUUUUCUCCUCGUAUCGCC
|
|
1244
UUUUCUCCUCGUAUCGCCA
|
|
1245
UUUCUCCUCGUAUCGCCAA
|
|
1246
UUCUCCUCGUAUCGCCAAA
|
|
1247
UCUCCUCGUAUCGCCAAAU
|
|
1248
CUCCUCGUAUCGCCAAAUU
|
|
1249
UCCUCGUAUCGCCAAAUUA
|
|
1250
CCUCGUAUCGCCAAAUUAA
|
|
1251
CUCGUAUCGCCAAAUUAAC
|
|
1252
UCGUAUCGCCAAAUUAACG
|
|
1253
CGUAUCGCCAAAUUAACGC
|
|
1254
GUAUCGCCAAAUUAACGCG
|
|
1255
UAUCGCCAAAUUAACGCGU
|
|
1256
AUCGCCAAAUUAACGCGUU
|
|
1257
UCGCCAAAUUAACGCGUUU
|
|
1258
CGCCAAAUUAACGCGUUUU
|
|
1259
GCCAAAUUAACGCGUUUUG
|
|
1260
CCAAAUUAACGCGUUUUGC
|
|
1261
CAAAUUAACGCGUUUUGCA
|
|
1262
AAAUUAACGCGUUUUGCAU
|
|
1263
AAUUAACGCGUUUUGCAUA
|
|
1264
AUUAACGCGUUUUGCAUAU
|
|
1265
UUAACGCGUUUUGCAUAUU
|
|
1266
UAACGCGUUUUGCAUAUUA
|
|
1267
AACGCGUUUUGCAUAUUAC
|
|
1268
ACGCGUUUUGCAUAUUACA
|
|
1269
CGCGUUUUGCAUAUUACAG
|
|
1270
GCGUUUUGCAUAUUACAGU
|
|
1271
CGUUUUGCAUAUUACAGUU
|
|
1272
GUUUUGCAUAUUACAGUUG
|
|
1273
UUUUGCAUAUUACAGUUGA
|
|
1274
UUUGCAUAUUACAGUUGAG
|
|
1275
UUGCAUAUUACAGUUGAGU
|
|
1276
UGCAUAUUACAGUUGAGUG
|
|
1277
GCAUAUUACAGUUGAGUGC
|
|
1278
CAUAUUACAGUUGAGUGCC
|
|
1279
AUAUUACAGUUGAGUGCCU
|
|
1280
UAUUACAGUUGAGUGCCUC
|
|
1281
AUUACAGUUGAGUGCCUCG
|
|
1282
UUACAGUUGAGUGCCUCGA
|
|
1283
UACAGUUGAGUGCCUCGAC
|
|
1284
ACAGUUGAGUGCCUCGACU
|
|
1285
CAGUUGAGUGCCUCGACUU
|
|
1286
AGUUGAGUGCCUCGACUUA
|
|
1287
GUUGAGUGCCUCGACUUAG
|
|
1288
UUGAGUGCCUCGACUUAGA
|
|
1289
UGAGUGCCUCGACUUAGAU
|
|
1290
GAGUGCCUCGACUUAGAUU
|
|
1291
AGUGCCUCGACUUAGAUUG
|
|
1292
GUGCCUCGACUUAGAUUGC
|
|
1293
UGCCUCGACUUAGAUUGCA
|
|
1294
GCCUCGACUUAGAUUGCAA
|
|
1295
CCUCGACUUAGAUUGCAAU
|
|
1296
CUCGACUUAGAUUGCAAUA
|
|
1297
UCGACUUAGAUUGCAAUAU
|
|
1298
CGACUUAGAUUGCAAUAUA
|
|
1299
GACUUAGAUUGCAAUAUAA
|
|
1300
ACUUAGAUUGCAAUAUAAG
|
|
1301
CUUAGAUUGCAAUAUAAGC
|
|
1302
UUAGAUUGCAAUAUAAGCG
|
|
1303
UAGAUUGCAAUAUAAGCGG
|
|
1304
AGAUUGCAAUAUAAGCGGC
|
|
1305
GAUUGCAAUAUAAGCGGCC
|
|
1306
AUUGCAAUAUAAGCGGCCA
|
|
1307
UUGCAAUAUAAGCGGCCAG
|
|
1308
UGCAAUAUAAGCGGCCAGC
|
|
1309
GCAAUAUAAGCGGCCAGCA
|
|
1310
CAAUAUAAGCGGCCAGCAA
|
|
1311
AAUAUAAGCGGCCAGCAAA
|
|
1312
AUAUAAGCGGCCAGCAAAC
|
|
1313
UAUAAGCGGCCAGCAAACA
|
|
1314
AUAAGCGGCCAGCAAACAA
|
|
1315
UAAGCGGCCAGCAAACAAG
|
|
1316
AAGCGGCCAGCAAACAAGU
|
|
1317
AGCGGCCAGCAAACAAGUC
|
|
1318
GCGGCCAGCAAACAAGUCU
|
|
1319
CGGCCAGCAAACAAGUCUC
|
|
1320
GGCCAGCAAACAAGUCUCA
|
|
1321
GCCAGCAAACAAGUCUCAA
|
|
1322
CCAGCAAACAAGUCUCAAA
|
|
1323
CAGCAAACAAGUCUCAAAA
|
|
1324
AGCAAACAAGUCUCAAAAA
|
|
1325
GCAAACAAGUCUCAAAAAA
|
|
1326
CAAACAAGUCUCAAAAAAA
|
|
1327
AAACAAGUCUCAAAAAAAA
|
|
1328
AACAAGUCUCAAAAAAAAG
|
|
1329
ACAAGUCUCAAAAAAAAGU
|
|
1330
CAAGUCUCAAAAAAAAGUU
|
|
1331
AAGUCUCAAAAAAAAGUUA
|
|
1332
AGUCUCAAAAAAAAGUUAC
|
|
1333
GUCUCAAAAAAAAGUUACG
|
|
1334
UCUCAAAAAAAAGUUACGU
|
|
1335
CUCAAAAAAAAGUUACGUG
|
|
1336
UCAAAAAAAAGUUACGUGC
|
|
1337
CAAAAAAAAGUUACGUGCG
|
|
1338
AAAAAAAAGUUACGUGCGU
|
|
1339
AAAAAAAGUUACGUGCGUU
|
|
1340
AAAAAAGUUACGUGCGUUU
|
|
1341
AAAAAGUUACGUGCGUUUC
|
|
1342
AAAAGUUACGUGCGUUUCU
|
|
1343
AAAGUUACGUGCGUUUCUG
|
|
1344
AAGUUACGUGCGUUUCUGC
|
|
1345
AGUUACGUGCGUUUCUGCG
|
|
1346
GUUACGUGCGUUUCUGCGA
|
|
1347
UUACGUGCGUUUCUGCGAG
|
|
1348
UACGUGCGUUUCUGCGAGU
|
|
1349
ACGUGCGUUUCUGCGAGUG
|
|
1350
CGUGCGUUUCUGCGAGUGU
|
|
1351
GUGCGUUUCUGCGAGUGUU
|
|
1352
UGCGUUUCUGCGAGUGUUA
|
|
1353
GCGUUUCUGCGAGUGUUAU
|
|
1354
CGUUUCUGCGAGUGUUAUU
|
|
1355
GUUUCUGCGAGUGUUAUUU
|
|
1356
UUUCUGCGAGUGUUAUUUU
|
|
1357
UUCUGCGAGUGUUAUUUUG
|
|
1358
UCUGCGAGUGUUAUUUUGU
|
|
1359
CUGCGAGUGUUAUUUUGUU
|
|
1360
UGCGAGUGUUAUUUUGUUA
|
|
1361
GCGAGUGUUAUUUUGUUAA
|
|
1362
CGAGUGUUAUUUUGUUAAG
|
|
1363
GAGUGUUAUUUUGUUAAGA
|
|
1364
AGUGUUAUUUUGUUAAGAA
|
|
1365
GUGUUAUUUUGUUAAGAAC
|
|
1366
UGUUAUUUUGUUAAGAACG
|
|
1367
GUUAUUUUGUUAAGAACGG
|
|
1368
UUAUUUUGUUAAGAACGGC
|
|
1369
UAUUUUGUUAAGAACGGCU
|
|
1370
AUUUUGUUAAGAACGGCUC
|
|
1371
UUUUGUUAAGAACGGCUCA
|
|
1372
UUUGUUAAGAACGGCUCAC
|
|
1373
UUGUUAAGAACGGCUCACA
|
|
1374
UGUUAAGAACGGCUCACAG
|
|
1375
GUUAAGAACGGCUCACAGU
|
|
1376
UUAAGAACGGCUCACAGUG
|
|
1377
UAAGAACGGCUCACAGUGU
|
|
1378
AAGAACGGCUCACAGUGUC
|
|
1379
AGAACGGCUCACAGUGUCC
|
|
1380
GAACGGCUCACAGUGUCCU
|
|
1381
AACGGCUCACAGUGUCCUC
|
|
1382
ACGGCUCACAGUGUCCUCU
|
|
1383
CGGCUCACAGUGUCCUCUU
|
|
1384
GGCUCACAGUGUCCUCUUC
|
|
1385
GCUCACAGUGUCCUCUUCC
|
|
1386
CUCACAGUGUCCUCUUCCU
|
|
1387
UCACAGUGUCCUCUUCCUG
|
|
1388
CACAGUGUCCUCUUCCUGU
|
|
1389
ACAGUGUCCUCUUCCUGUG
|
|
1390
CAGUGUCCUCUUCCUGUGU
|
|
1391
AGUGUCCUCUUCCUGUGUU
|
|
1392
GUGUCCUCUUCCUGUGUUA
|
|
1393
UGUCCUCUUCCUGUGUUAC
|
|
1394
GUCCUCUUCCUGUGUUACA
|
|
1395
UCCUCUUCCUGUGUUACAG
|
|
1396
CCUCUUCCUGUGUUACAGA
|
|
1397
CUCUUCCUGUGUUACAGAA
|
|
1398
UCUUCCUGUGUUACAGAAG
|
|
1399
CUUCCUGUGUUACAGAAGC
|
|
1400
UUCCUGUGUUACAGAAGCC
|
|
1401
UCCUGUGUUACAGAAGCCA
|
|
1402
CCUGUGUUACAGAAGCCAA
|
|
1403
CUGUGUUACAGAAGCCAAC
|
|
1404
UGUGUUACAGAAGCCAACC
|
|
1405
GUGUUACAGAAGCCAACCU
|
|
1406
UGUUACAGAAGCCAACCUG
|
|
1407
GUUACAGAAGCCAACCUGA
|
|
1408
UUACAGAAGCCAACCUGAA
|
|
1409
UACAGAAGCCAACCUGAAA
|
|
1410
ACAGAAGCCAACCUGAAAU
|
|
1411
CAGAAGCCAACCUGAAAUG
|
|
1412
AGAAGCCAACCUGAAAUGA
|
|
1413
GAAGCCAACCUGAAAUGAA
|
|
1414
AAGCCAACCUGAAAUGAAA
|
|
1415
AGCCAACCUGAAAUGAAAC
|
|
1416
GCCAACCUGAAAUGAAACU
|
|
1417
CCAACCUGAAAUGAAACUA
|
|
1418
CAACCUGAAAUGAAACUAG
|
|
1419
AACCUGAAAUGAAACUAGU
|
|
1420
ACCUGAAAUGAAACUAGUC
|
|
1421
CCUGAAAUGAAACUAGUCU
|
|
1422
CUGAAAUGAAACUAGUCUG
|
|
1423
UGAAAUGAAACUAGUCUGG
|
|
1424
GAAAUGAAACUAGUCUGGA
|
|
1425
AAAUGAAACUAGUCUGGAA
|
|
1426
AAUGAAACUAGUCUGGAAA
|
|
1427
AUGAAACUAGUCUGGAAAA
|
|
1428
UGAAACUAGUCUGGAAAAA
|
|
1429
GAAACUAGUCUGGAAAAAU
|
|
1430
AAACUAGUCUGGAAAAAUU
|
|
1431
AACUAGUCUGGAAAAAUUC
|
|
1432
ACUAGUCUGGAAAAAUUCA
|
|
1433
CUAGUCUGGAAAAAUUCAU
|
|
1434
UAGUCUGGAAAAAUUCAUU
|
|
1435
AGUCUGGAAAAAUUCAUUG
|
|
1436
GUCUGGAAAAAUUCAUUGU
|
|
1437
UCUGGAAAAAUUCAUUGUU
|
|
1438
CUGGAAAAAUUCAUUGUUC
|
|
1439
UGGAAAAAUUCAUUGUUCU
|
|
1440
GGAAAAAUUCAUUGUUCUC
|
|
1441
GAAAAAUUCAUUGUUCUCU
|
|
1442
AAAAAUUCAUUGUUCUCUG
|
|
1443
AAAAUUCAUUGUUCUCUGU
|
|
1444
AAAUUCAUUGUUCUCUGUA
|
|
1445
AAUUCAUUGUUCUCUGUAG
|
|
1446
AUUCAUUGUUCUCUGUAGU
|
|
1447
UUCAUUGUUCUCUGUAGUU
|
|
1448
UCAUUGUUCUCUGUAGUUG
|
|
1449
CAUUGUUCUCUGUAGUUGC
|
|
1450
AUUGUUCUCUGUAGUUGCA
|
|
1451
UUGUUCUCUGUAGUUGCAG
|
|
1452
UGUUCUCUGUAGUUGCAGC
|
|
1453
GUUCUCUGUAGUUGCAGCU
|
|
1454
UUCUCUGUAGUUGCAGCUG
|
|
1455
UCUCUGUAGUUGCAGCUGU
|
|
1456
CUCUGUAGUUGCAGCUGUA
|
|
1457
UCUGUAGUUGCAGCUGUAC
|
|
1458
CUGUAGUUGCAGCUGUACC
|
|
1459
UGUAGUUGCAGCUGUACCU
|
|
1460
GUAGUUGCAGCUGUACCUG
|
|
1461
UAGUUGCAGCUGUACCUGA
|
|
1462
AGUUGCAGCUGUACCUGAA
|
|
1463
GUUGCAGCUGUACCUGAAA
|
|
1464
UUGCAGCUGUACCUGAAAU
|
|
1465
UGCAGCUGUACCUGAAAUA
|
|
1466
GCAGCUGUACCUGAAAUAA
|
|
1467
CAGCUGUACCUGAAAUAAA
|
|
1468
AGCUGUACCUGAAAUAAAA
|
|
1469
GCUGUACCUGAAAUAAAAA
|
|
1470
CUGUACCUGAAAUAAAAAU
|
|
1471
UGUACCUGAAAUAAAAAUG
|
|
1472
GUACCUGAAAUAAAAAUGU
|
|
1473
UACCUGAAAUAAAAAUGUU
|
|
1474
ACCUGAAAUAAAAAUGUUA
|
|
1475
CCUGAAAUAAAAAUGUUAU
|
|
1476
CUGAAAUAAAAAUGUUAUU
|
|
1477
UGAAAUAAAAAUGUUAUUG
|
|
1478
GAAAUAAAAAUGUUAUUGA
|
|
1479
AAAUAAAAAUGUUAUUGAU
|
|
1480
AAUAAAAAUGUUAUUGAUG
|
|
1481
AUAAAAAUGUUAUUGAUGA
|
|
1482
UAAAAAUGUUAUUGAUGAC
|
|
1483
AAAAAUGUUAUUGAUGACU
|
|
1484
AAAAUGUUAUUGAUGACUG
|
|
1485
AAAUGUUAUUGAUGACUGA
|
|
1486
AAUGUUAUUGAUGACUGAA
|
|
1487
AUGUUAUUGAUGACUGAAA
|
|
1488
UGUUAUUGAUGACUGAAAA
|
|
1489
GUUAUUGAUGACUGAAAAA
|
|
1490
UUAUUGAUGACUGAAAAAA
|
|
1491
UAUUGAUGACUGAAAAAAA
|
|
1492
AUUGAUGACUGAAAAAAAA
|
|
1493
UUGAUGACUGAAAAAAAA
|
|
1494
UGAUGACUGAAAAAAAAA
|
|
1495
GAUGACUGAAAAAAAAAA
|
|
1496
AUGACUGAAAAAAAAAAA
|
|
1497
UGACUGAAAAAAAAAAAA
|
|
1498
GACUGAAAAAAAAAAAAA
|
|
1499
ACUGAAAAAAAAAAAAAA
|
|
1500
CUGAAAAAAAAAAAAAAA
|
|
1501
UGAAAAAAAAAAAAAAAA
|
|
1502
AAAACAGUGGCAGAUGAAA
|
|
1503
AAACAGUGGCAGAUGAAAG
|
|
1504
AACAGUGGCAGAUGAAAGG
|
|
1505
ACAGUGGCAGAUGAAAGGG
|
|
1506
CAGUGGCAGAUGAAAGGGA
|
|
1507
AGUGGCAGAUGAAAGGGAU
|
|
1508
GUGGCAGAUGAAAGGGAUG
|
|
1509
UGGCAGAUGAAAGGGAUGG
|
|
1510
GGCAGAUGAAAGGGAUGGA
|
|
1511
GCAGAUGAAAGGGAUGGAG
|
|
1512
CAGAUGAAAGGGAUGGAGA
|
|
1513
AGAUGAAAGGGAUGGAGAA
|
|
1514
GAUGAAAGGGAUGGAGAAC
|
|
1515
AUGAAAGGGAUGGAGAACC
|
|
1516
UGAAAGGGAUGGAGAACCG
|
|
1517
GAAAGGGAUGGAGAACCGC
|
|
1518
AAAGGGAUGGAGAACCGCC
|
|
1519
AAGGGAUGGAGAACCGCCA
|
|
1520
AGGGAUGGAGAACCGCCAU
|
|
1521
GGGAUGGAGAACCGCCAUG
|
|
1522
GGAUGGAGAACCGCCAUGC
|
|
1523
GAUGGAGAACCGCCAUGCC
|
|
1524
AUGCCCGGUGACUCCCCGG
|
|
1525
UGCCCGGUGACUCCCCGGC
|
|
1526
GCCCGGUGACUCCCCGGCC
|
|
1527
CCCGGUGACUCCCCGGCCG
|
|
1528
CCGGUGACUCCCCGGCCGU
|
|
1529
CGGUGACUCCCCGGCCGUC
|
|
1530
GGUGACUCCCCGGCCGUCA
|
|
1531
GUGACUCCCCGGCCGUCAG
|
|
1532
UGACUCCCCGGCCGUCAGC
|
|
1533
GACUCCCCGGCCGUCAGCA
|
|
1534
ACUCCCCGGCCGUCAGCAA
|
|
1535
CUCCCCGGCCGUCAGCAAG
|
|
1536
UCCCCGGCCGUCAGCAAGC
|
|
1537
CCCCGGCCGUCAGCAAGCC
|
|
1538
CCCGGCCGUCAGCAAGCCC
|
|
1539
CCGGCCGUCAGCAAGCCCC
|
|
1540
CGGCCGUCAGCAAGCCCCC
|
|
1541
GGCCGUCAGCAAGCCCCCG
|
|
1542
GCCGUCAGCAAGCCCCCGG
|
|
1543
CCGUCAGCAAGCCCCCGGA
|
|
1544
CGUCAGCAAGCCCCCGGAC
|
|
1545
GUCAGCAAGCCCCCGGACG
|
|
1546
UCAGCAAGCCCCCGGACGG
|
|
1547
CAGCAAGCCCCCGGACGGC
|
|
1548
AGCAAGCCCCCGGACGGCG
|
|
1549
GCAAGCCCCCGGACGGCGC
|
|
1550
CAAGCCCCCGGACGGCGCC
|
|
1551
AAGCCCCCGGACGGCGCCG
|
|
1552
AGCCCCCGGACGGCGCCGG
|
|
1553
GCCCCCGGACGGCGCCGGG
|
|
1554
CCCCCGGACGGCGCCGGGC
|
|
1555
CCCCGGACGGCGCCGGGCC
|
|
1556
CCCGGACGGCGCCGGGCCG
|
|
1557
CCGGACGGCGCCGGGCCGG
|
|
1558
CGGACGGCGCCGGGCCGGG
|
|
1559
GGACGGCGCCGGGCCGGGG
|
|
1560
GACGGCGCCGGGCCGGGGG
|
|
1561
ACGGCGCCGGGCCGGGGGA
|
|
1562
CGGCGCCGGGCCGGGGGAC
|
|
1563
GGCGCCGGGCCGGGGGACA
|
|
1564
GCGCCGGGCCGGGGGACAA
|
|
1565
CGCCGGGCCGGGGGACAAG
|
|
1566
GCCGGGCCGGGGGACAAGG
|
|
1567
CCGGGCCGGGGGACAAGGC
|
|
1568
CGGGCCGGGGGACAAGGCG
|
|
1569
GGGCCGGGGGACAAGGCGG
|
|
1570
GGCCGGGGGACAAGGCGGG
|
|
1571
GCCGGGGGACAAGGCGGGC
|
|
1572
CCGGGGGACAAGGCGGGCG
|
|
1573
CGGGGGACAAGGCGGGCGG
|
|
1574
GGGGGACAAGGCGGGCGGC
|
|
1575
GGGGACAAGGCGGGCGGCC
|
|
1576
GGGACAAGGCGGGCGGCCU
|
|
1577
GGACAAGGCGGGCGGCCUC
|
|
1578
GACAAGGCGGGCGGCCUCG
|
|
1579
ACAAGGCGGGCGGCCUCGG
|
|
1580
CAAGGCGGGCGGCCUCGGC
|
|
1581
AAGGCGGGCGGCCUCGGCA
|
|
1582
AGGCGGGCGGCCUCGGCAA
|
|
1583
GGCGGGCGGCCUCGGCAAG
|
|
1584
GCGGGCGGCCUCGGCAAGG
|
|
1585
CGGGCGGCCUCGGCAAGGC
|
|
1586
GGGCGGCCUCGGCAAGGCG
|
|
1587
GGCGGCCUCGGCAAGGCGG
|
|
1588
GCGGCCUCGGCAAGGCGGC
|
|
1589
CGGCCUCGGCAAGGCGGCG
|
|
1590
GGCCUCGGCAAGGCGGCGG
|
|
1591
GCCUCGGCAAGGCGGCGGC
|
|
1592
CCUCGGCAAGGCGGCGGCC
|
|
1593
CUCGGCAAGGCGGCGGCCC
|
|
1594
UCGGCAAGGCGGCGGCCCA
|
|
1595
CGGCAAGGCGGCGGCCCAA
|
|
1596
GGCAAGGCGGCGGCCCAAA
|
|
1597
GCAAGGCGGCGGCCCAAAU
|
|
1598
CAAGGCGGCGGCCCAAAUG
|
|
1599
AAGGCGGCGGCCCAAAUGG
|
|
1600
AGGCGGCGGCCCAAAUGGC
|
|
1601
GGCGGCGGCCCAAAUGGCG
|
|
1602
GCGGCGGCCCAAAUGGCGG
|
|
1603
CGGCGGCCCAAAUGGCGGC
|
|
1604
GGCGGCCCAAAUGGCGGCC
|
|
1605
GCGGCCCAAAUGGCGGCCG
|
|
1606
CGGCCCAAAUGGCGGCCGC
|
|
1607
GGCCCAAAUGGCGGCCGCC
|
|
1608
GCCCAAAUGGCGGCCGCCC
|
|
1609
CCCAAAUGGCGGCCGCCCC
|
|
1610
CCAAAUGGCGGCCGCCCCG
|
|
1611
CAAAUGGCGGCCGCCCCGG
|
|
1612
AAAUGGCGGCCGCCCCGGC
|
|
1613
AAUGGCGGCCGCCCCGGCG
|
|
1614
AUGGCGGCCGCCCCGGCGG
|
|
1615
UGGCGGCCGCCCCGGCGGC
|
|
1616
GGCGGCCGCCCCGGCGGCG
|
|
1617
GCGGCCGCCCCGGCGGCGG
|
|
1618
CGGCCGCCCCGGCGGCGGG
|
|
1619
GGCCGCCCCGGCGGCGGGC
|
|
1620
GCCGCCCCGGCGGCGGGCA
|
|
1621
CCGCCCCGGCGGCGGGCAA
|
|
1622
CGCCCCGGCGGCGGGCAAG
|
|
1623
GCCCCGGCGGCGGGCAAGA
|
|
1624
CCCCGGCGGCGGGCAAGAA
|
|
1625
CCCGGCGGCGGGCAAGAAG
|
|
1626
GGCAGCGGGUGAUGGCCGC
|
|
1627
GCAGCGGGUGAUGGCCGCG
|
|
1628
CAGCGGGUGAUGGCCGCGC
|
|
1629
AGCGGGUGAUGGCCGCGCA
|
|
1630
GCGGGUGAUGGCCGCGCAG
|
|
1631
CGGGUGAUGGCCGCGCAGG
|
|
1632
GGGUGAUGGCCGCGCAGGU
|
|
1633
GGUGAUGGCCGCGCAGGUU
|
|
1634
GUGAUGGCCGCGCAGGUUG
|
|
1635
UGAUGGCCGCGCAGGUUGC
|
|
1636
GAUGGCCGCGCAGGUUGCA
|
|
1637
AUGGCCGCGCAGGUUGCAC
|
|
1638
UGGCCGCGCAGGUUGCACU
|
|
1639
GGCCGCGCAGGUUGCACUG
|
|
1640
GCCGCGCAGGUUGCACUGA
|
|
1641
CCGCGCAGGUUGCACUGAG
|
|
1642
CGCGCAGGUUGCACUGAGG
|
|
1643
GCGCAGGUUGCACUGAGGA
|
|
1644
CGCAGGUUGCACUGAGGAG
|
|
TABLE 2
|
|
DsRNA molecules targeting mRNA encoding chicken
|
ASW (WPKCI).
|
SEQ ID NO
Sequence 5′-3′
|
|
1645
CGUCUGCGGGUGCCUUGCG
|
|
1646
GUCUGCGGGUGCCUUGCGA
|
|
1647
UCUGCGGGUGCCUUGCGAU
|
|
1648
CUGCGGGUGCCUUGCGAUA
|
|
1649
UGCGGGUGCCUUGCGAUAC
|
|
1650
GCGGGUGCCUUGCGAUACG
|
|
1651
CGGGUGCCUUGCGAUACGU
|
|
1652
GGGUGCCUUGCGAUACGUC
|
|
1653
GGUGCCUUGCGAUACGUCG
|
|
1654
GUGCCUUGCGAUACGUCGG
|
|
1655
UGCCUUGCGAUACGUCGGC
|
|
1656
GCCUUGCGAUACGUCGGCA
|
|
1657
CCUUGCGAUACGUCGGCAU
|
|
1658
CUUGCGAUACGUCGGCAUG
|
|
1659
UUGCGAUACGUCGGCAUGG
|
|
1660
UGCGAUACGUCGGCAUGGC
|
|
1661
GCGAUACGUCGGCAUGGCU
|
|
1662
CGAUACGUCGGCAUGGCUG
|
|
1663
GAUACGUCGGCAUGGCUGA
|
|
1664
AUACGUCGGCAUGGCUGAC
|
|
1665
UACGUCGGCAUGGCUGACG
|
|
1666
ACGUCGGCAUGGCUGACGA
|
|
1667
CGUCGGCAUGGCUGACGAG
|
|
1668
GUCGGCAUGGCUGACGAGA
|
|
1669
UCGGCAUGGCUGACGAGAU
|
|
1670
CGGCAUGGCUGACGAGAUC
|
|
1671
GGCAUGGCUGACGAGAUCC
|
|
1672
GCAUGGCUGACGAGAUCCG
|
|
1673
CAUGGCUGACGAGAUCCGC
|
|
1674
AUGGCUGACGAGAUCCGCA
|
|
1675
UGGCUGACGAGAUCCGCAA
|
|
1676
GGCUGACGAGAUCCGCAAG
|
|
1677
GCUGACGAGAUCCGCAAGG
|
|
1678
CUGACGAGAUCCGCAAGGC
|
|
1679
UGACGAGAUCCGCAAGGCG
|
|
1680
GACGAGAUCCGCAAGGCGC
|
|
1681
ACGAGAUCCGCAAGGCGCA
|
|
1682
CGAGAUCCGCAAGGCGCAG
|
|
1683
GAGAUCCGCAAGGCGCAGG
|
|
1684
AGAUCCGCAAGGCGCAGGC
|
|
1685
GAUCCGCAAGGCGCAGGCC
|
|
1686
AUCCGCAAGGCGCAGGCCG
|
|
1687
UCCGCAAGGCGCAGGCCGC
|
|
1688
CCGCAAGGCGCAGGCCGCG
|
|
1689
CGCAAGGCGCAGGCCGCGC
|
|
1690
GCAAGGCGCAGGCCGCGCG
|
|
1691
CAAGGCGCAGGCCGCGCGC
|
|
1692
AAGGCGCAGGCCGCGCGCC
|
|
1693
AGGCGCAGGCCGCGCGCCC
|
|
1694
GGCGCAGGCCGCGCGCCCU
|
|
1695
GCGCAGGCCGCGCGCCCUG
|
|
1696
CGCAGGCCGCGCGCCCUGG
|
|
1697
GCAGGCCGCGCGCCCUGGU
|
|
1698
CAGGCCGCGCGCCCUGGUG
|
|
1699
AGGCCGCGCGCCCUGGUGG
|
|
1700
GGCCGCGCGCCCUGGUGGG
|
|
1701
GCCGCGCGCCCUGGUGGGG
|
|
1702
CCGCGCGCCCUGGUGGGGA
|
|
1703
CGCGCGCCCUGGUGGGGAC
|
|
1704
GCGCGCCCUGGUGGGGACA
|
|
1705
CGCGCCCUGGUGGGGACAC
|
|
1706
GCGCCCUGGUGGGGACACC
|
|
1707
CGCCCUGGUGGGGACACCA
|
|
1708
GCCCUGGUGGGGACACCAU
|
|
1709
CCCUGGUGGGGACACCAUC
|
|
1710
CCUGGUGGGGACACCAUCU
|
|
1711
CUGGUGGGGACACCAUCUU
|
|
1712
UGGUGGGGACACCAUCUUC
|
|
1713
GGUGGGGACACCAUCUUCG
|
|
1714
GUGGGGACACCAUCUUCGG
|
|
1715
UGGGGACACCAUCUUCGGG
|
|
1716
GGGGACACCAUCUUCGGGA
|
|
1717
GGGACACCAUCUUCGGGAA
|
|
1718
GGACACCAUCUUCGGGAAG
|
|
1719
GACACCAUCUUCGGGAAGA
|
|
1720
ACACCAUCUUCGGGAAGAU
|
|
1721
CACCAUCUUCGGGAAGAUU
|
|
1722
ACCAUCUUCGGGAAGAUUA
|
|
1723
CCAUCUUCGGGAAGAUUAU
|
|
1724
CAUCUUCGGGAAGAUUAUC
|
|
1725
AUCUUCGGGAAGAUUAUCC
|
|
1726
UCUUCGGGAAGAUUAUCCG
|
|
1727
CUUCGGGAAGAUUAUCCGC
|
|
1728
UUCGGGAAGAUUAUCCGCA
|
|
1729
UCGGGAAGAUUAUCCGCAA
|
|
1730
CGGGAAGAUUAUCCGCAAG
|
|
1731
GGGAAGAUUAUCCGCAAGG
|
|
1732
GGAAGAUUAUCCGCAAGGA
|
|
1733
GAAGAUUAUCCGCAAGGAG
|
|
1734
AAGAUUAUCCGCAAGGAGA
|
|
1735
AGAUUAUCCGCAAGGAGAU
|
|
1736
GAUUAUCCGCAAGGAGAUU
|
|
1737
AUUAUCCGCAAGGAGAUUC
|
|
1738
UUAUCCGCAAGGAGAUUCC
|
|
1739
UAUCCGCAAGGAGAUUCCC
|
|
1740
AUCCGCAAGGAGAUUCCCG
|
|
1741
UCCGCAAGGAGAUUCCCGC
|
|
1742
CCGCAAGGAGAUUCCCGCC
|
|
1743
CGCAAGGAGAUUCCCGCCA
|
|
1744
GCAAGGAGAUUCCCGCCAA
|
|
1745
CAAGGAGAUUCCCGCCAAC
|
|
1746
AAGGAGAUUCCCGCCAACA
|
|
1747
AGGAGAUUCCCGCCAACAU
|
|
1748
GGAGAUUCCCGCCAACAUA
|
|
1749
GAGAUUCCCGCCAACAUAA
|
|
1750
AGAUUCCCGCCAACAUAAU
|
|
1751
GAUUCCCGCCAACAUAAUC
|
|
1752
AUUCCCGCCAACAUAAUCU
|
|
1753
UUCCCGCCAACAUAAUCUA
|
|
1754
UCCCGCCAACAUAAUCUAC
|
|
1755
CCCGCCAACAUAAUCUACG
|
|
1756
CCGCCAACAUAAUCUACGA
|
|
1757
CGCCAACAUAAUCUACGAG
|
|
1758
GCCAACAUAAUCUACGAGG
|
|
1759
CCAACAUAAUCUACGAGGA
|
|
1760
CAACAUAAUCUACGAGGAC
|
|
1761
AACAUAAUCUACGAGGACG
|
|
1762
ACAUAAUCUACGAGGACGA
|
|
1763
CAUAAUCUACGAGGACGAG
|
|
1764
AUAAUCUACGAGGACGAGC
|
|
1765
UAAUCUACGAGGACGAGCA
|
|
1766
AAUCUACGAGGACGAGCAG
|
|
1767
AUCUACGAGGACGAGCAGU
|
|
1768
UCUACGAGGACGAGCAGUG
|
|
1769
CUACGAGGACGAGCAGUGC
|
|
1770
UACGAGGACGAGCAGUGCC
|
|
1771
ACGAGGACGAGCAGUGCCU
|
|
1772
CGAGGACGAGCAGUGCCUU
|
|
1773
GAGGACGAGCAGUGCCUUG
|
|
1774
AGGACGAGCAGUGCCUUGC
|
|
1775
GGACGAGCAGUGCCUUGCG
|
|
1776
GACGAGCAGUGCCUUGCGU
|
|
1777
ACGAGCAGUGCCUUGCGUU
|
|
1778
CGAGCAGUGCCUUGCGUUC
|
|
1779
GAGCAGUGCCUUGCGUUCC
|
|
1780
AGCAGUGCCUUGCGUUCCA
|
|
1781
GCAGUGCCUUGCGUUCCAU
|
|
1782
CAGUGCCUUGCGUUCCAUG
|
|
1783
AGUGCCUUGCGUUCCAUGA
|
|
1784
GUGCCUUGCGUUCCAUGAU
|
|
1785
UGCCUUGCGUUCCAUGAUA
|
|
1786
GCCUUGCGUUCCAUGAUAU
|
|
1787
CCUUGCGUUCCAUGAUAUC
|
|
1788
CUUGCGUUCCAUGAUAUCU
|
|
1789
UUGCGUUCCAUGAUAUCUC
|
|
1790
UGCGUUCCAUGAUAUCUCA
|
|
1791
GCGUUCCAUGAUAUCUCAC
|
|
1792
CGUUCCAUGAUAUCUCACC
|
|
1793
GUUCCAUGAUAUCUCACCC
|
|
1794
UUCCAUGAUAUCUCACCCC
|
|
1795
UCCAUGAUAUCUCACCCCA
|
|
1796
CCAUGAUAUCUCACCCCAA
|
|
1797
CAUGAUAUCUCACCCCAAG
|
|
1798
AUGAUAUCUCACCCCAAGC
|
|
1799
UGAUAUCUCACCCCAAGCU
|
|
1800
GAUAUCUCACCCCAAGCUC
|
|
1801
AUAUCUCACCCCAAGCUCC
|
|
1802
UAUCUCACCCCAAGCUCCA
|
|
1803
AUCUCACCCCAAGCUCCAA
|
|
1804
UCUCACCCCAAGCUCCAAC
|
|
1805
CUCACCCCAAGCUCCAACG
|
|
1806
UCACCCCAAGCUCCAACGC
|
|
1807
CACCCCAAGCUCCAACGCA
|
|
1808
ACCCCAAGCUCCAACGCAU
|
|
1809
CCCCAAGCUCCAACGCAUU
|
|
1810
CCCAAGCUCCAACGCAUUU
|
|
1811
CCAAGCUCCAACGCAUUUU
|
|
1812
CAAGCUCCAACGCAUUUUC
|
|
1813
AAGCUCCAACGCAUUUUCU
|
|
1814
AGCUCCAACGCAUUUUCUA
|
|
1815
GCUCCAACGCAUUUUCUAG
|
|
1816
CUCCAACGCAUUUUCUAGU
|
|
1817
UCCAACGCAUUUUCUAGUG
|
|
1818
CCAACGCAUUUUCUAGUGA
|
|
1819
CAACGCAUUUUCUAGUGAU
|
|
1820
AACGCAUUUUCUAGUGAUU
|
|
1821
ACGCAUUUUCUAGUGAUUC
|
|
1822
CGCAUUUUCUAGUGAUUCC
|
|
1823
GCAUUUUCUAGUGAUUCCU
|
|
1824
CAUUUUCUAGUGAUUCCUA
|
|
1825
AUUUUCUAGUGAUUCCUAA
|
|
1826
UUUUCUAGUGAUUCCUAAG
|
|
1827
UUUCUAGUGAUUCCUAAGA
|
|
1828
UUCUAGUGAUUCCUAAGAA
|
|
1829
UCUAGUGAUUCCUAAGAAG
|
|
1830
CUAGUGAUUCCUAAGAAGC
|
|
1831
UAGUGAUUCCUAAGAAGCC
|
|
1832
AGUGAUUCCUAAGAAGCCA
|
|
1833
GUGAUUCCUAAGAAGCCAA
|
|
1834
UGAUUCCUAAGAAGCCAAU
|
|
1835
GAUUCCUAAGAAGCCAAUU
|
|
1836
AUUCCUAAGAAGCCAAUUG
|
|
1837
UUCCUAAGAAGCCAAUUGU
|
|
1838
UCCUAAGAAGCCAAUUGUC
|
|
1839
CCUAAGAAGCCAAUUGUCA
|
|
1840
CUAAGAAGCCAAUUGUCAG
|
|
1841
UAAGAAGCCAAUUGUCAGG
|
|
1842
AAGAAGCCAAUUGUCAGGU
|
|
1843
AGAAGCCAAUUGUCAGGUU
|
|
1844
GAAGCCAAUUGUCAGGUUA
|
|
1845
AAGCCAAUUGUCAGGUUAU
|
|
1846
AGCCAAUUGUCAGGUUAUC
|
|
1847
GCCAAUUGUCAGGUUAUCU
|
|
1848
CCAAUUGUCAGGUUAUCUG
|
|
1849
CAAUUGUCAGGUUAUCUGA
|
|
1850
AAUUGUCAGGUUAUCUGAA
|
|
1851
AUUGUCAGGUUAUCUGAAG
|
|
1852
UUGUCAGGUUAUCUGAAGC
|
|
1853
UGUCAGGUUAUCUGAAGCA
|
|
1854
GUCAGGUUAUCUGAAGCAG
|
|
1855
UCAGGUUAUCUGAAGCAGA
|
|
1856
CAGGUUAUCUGAAGCAGAA
|
|
1857
AGGUUAUCUGAAGCAGAAG
|
|
1858
GGUUAUCUGAAGCAGAAGA
|
|
1859
GUUAUCUGAAGCAGAAGAU
|
|
1860
UUAUCUGAAGCAGAAGAUU
|
|
1861
UAUCUGAAGCAGAAGAUUC
|
|
1862
AUCUGAAGCAGAAGAUUCU
|
|
1863
UCUGAAGCAGAAGAUUCUG
|
|
1864
CUGAAGCAGAAGAUUCUGA
|
|
1865
UGAAGCAGAAGAUUCUGAU
|
|
1866
GAAGCAGAAGAUUCUGAUG
|
|
1867
AAGCAGAAGAUUCUGAUGA
|
|
1868
AGCAGAAGAUUCUGAUGAA
|
|
1869
GCAGAAGAUUCUGAUGAAU
|
|
1870
CAGAAGAUUCUGAUGAAUC
|
|
1871
AGAAGAUUCUGAUGAAUCU
|
|
1872
GAAGAUUCUGAUGAAUCUC
|
|
1873
AAGAUUCUGAUGAAUCUCU
|
|
1874
AGAUUCUGAUGAAUCUCUU
|
|
1875
GAUUCUGAUGAAUCUCUUC
|
|
1876
AUUCUGAUGAAUCUCUUCU
|
|
1877
UUCUGAUGAAUCUCUUCUG
|
|
1878
UCUGAUGAAUCUCUUCUGG
|
|
1879
CUGAUGAAUCUCUUCUGGG
|
|
1880
UGAUGAAUCUCUUCUGGGG
|
|
1881
GAUGAAUCUCUUCUGGGGC
|
|
1882
AUGAAUCUCUUCUGGGGCA
|
|
1883
UGAAUCUCUUCUGGGGCAU
|
|
1884
GAAUCUCUUCUGGGGCAUU
|
|
1885
AAUCUCUUCUGGGGCAUUU
|
|
1886
AUCUCUUCUGGGGCAUUUA
|
|
1887
UCUCUUCUGGGGCAUUUAA
|
|
1888
CUCUUCUGGGGCAUUUAAU
|
|
1889
UCUUCUGGGGCAUUUAAUG
|
|
1890
CUUCUGGGGCAUUUAAUGA
|
|
1891
UUCUGGGGCAUUUAAUGAU
|
|
1892
UCUGGGGCAUUUAAUGAUU
|
|
1893
CUGGGGCAUUUAAUGAUUG
|
|
1894
UGGGGCAUUUAAUGAUUGU
|
|
1895
GGGGCAUUUAAUGAUUGUU
|
|
1896
GGGCAUUUAAUGAUUGUUG
|
|
1897
GGCAUUUAAUGAUUGUUGG
|
|
1898
GCAUUUAAUGAUUGUUGGC
|
|
1899
CAUUUAAUGAUUGUUGGCA
|
|
1900
AUUUAAUGAUUGUUGGCAA
|
|
1901
UUUAAUGAUUGUUGGCAAG
|
|
1902
UUAAUGAUUGUUGGCAAGA
|
|
1903
UAAUGAUUGUUGGCAAGAA
|
|
1904
AAUGAUUGUUGGCAAGAAG
|
|
1905
AUGAUUGUUGGCAAGAAGU
|
|
1906
UGAUUGUUGGCAAGAAGUG
|
|
1907
GAUUGUUGGCAAGAAGUGU
|
|
1908
AUUGUUGGCAAGAAGUGUG
|
|
1909
UUGUUGGCAAGAAGUGUGC
|
|
1910
UGUUGGCAAGAAGUGUGCU
|
|
1911
GUUGGCAAGAAGUGUGCUG
|
|
1912
UUGGCAAGAAGUGUGCUGC
|
|
1913
UGGCAAGAAGUGUGCUGCU
|
|
1914
GGCAAGAAGUGUGCUGCUA
|
|
1915
GCAAGAAGUGUGCUGCUAA
|
|
1916
CAAGAAGUGUGCUGCUAAC
|
|
1917
AAGAAGUGUGCUGCUAACC
|
|
1918
AGAAGUGUGCUGCUAACCU
|
|
1919
GAAGUGUGCUGCUAACCUG
|
|
1920
AAGUGUGCUGCUAACCUGG
|
|
1921
AGUGUGCUGCUAACCUGGG
|
|
1922
GUGUGCUGCUAACCUGGGC
|
|
1923
UGUGCUGCUAACCUGGGCC
|
|
1924
GUGCUGCUAACCUGGGCCU
|
|
1925
UGCUGCUAACCUGGGCCUG
|
|
1926
GCUGCUAACCUGGGCCUGA
|
|
1927
CUGCUAACCUGGGCCUGAC
|
|
1928
UGCUAACCUGGGCCUGACC
|
|
1929
GCUAACCUGGGCCUGACCA
|
|
1930
CUAACCUGGGCCUGACCAA
|
|
1931
UAACCUGGGCCUGACCAAU
|
|
1932
AACCUGGGCCUGACCAAUG
|
|
1933
ACCUGGGCCUGACCAAUGG
|
|
1934
CCUGGGCCUGACCAAUGGA
|
|
1935
CUGGGCCUGACCAAUGGAU
|
|
1936
UGGGCCUGACCAAUGGAUU
|
|
1937
GGGCCUGACCAAUGGAUUC
|
|
1938
GGCCUGACCAAUGGAUUCC
|
|
1939
GCCUGACCAAUGGAUUCCG
|
|
1940
CCUGACCAAUGGAUUCCGG
|
|
1941
CUGACCAAUGGAUUCCGGA
|
|
1942
UGACCAAUGGAUUCCGGAU
|
|
1943
GACCAAUGGAUUCCGGAUG
|
|
1944
ACCAAUGGAUUCCGGAUGG
|
|
1945
CCAAUGGAUUCCGGAUGGU
|
|
1946
CAAUGGAUUCCGGAUGGUU
|
|
1947
AAUGGAUUCCGGAUGGUUU
|
|
1948
AUGGAUUCCGGAUGGUUUU
|
|
1949
UGGAUUCCGGAUGGUUUUG
|
|
1950
GGAUUCCGGAUGGUUUUGA
|
|
1951
GAUUCCGGAUGGUUUUGAA
|
|
1952
AUUCCGGAUGGUUUUGAAU
|
|
1953
UUCCGGAUGGUUUUGAAUG
|
|
1954
UCCGGAUGGUUUUGAAUGA
|
|
1955
CCGGAUGGUUUUGAAUGAA
|
|
1956
CGGAUGGUUUUGAAUGAAG
|
|
1957
GGAUGGUUUUGAAUGAAGG
|
|
1958
GAUGGUUUUGAAUGAAGGG
|
|
1959
AUGGUUUUGAAUGAAGGGC
|
|
1960
UGGUUUUGAAUGAAGGGCC
|
|
1961
GGUUUUGAAUGAAGGGCCU
|
|
1962
GUUUUGAAUGAAGGGCCUG
|
|
1963
UUUUGAAUGAAGGGCCUGA
|
|
1964
UUUGAAUGAAGGGCCUGAG
|
|
1965
UUGAAUGAAGGGCCUGAGG
|
|
1966
UGAAUGAAGGGCCUGAGGG
|
|
1967
GAAUGAAGGGCCUGAGGGU
|
|
1968
AAUGAAGGGCCUGAGGGUG
|
|
1969
AUGAAGGGCCUGAGGGUGG
|
|
1970
UGAAGGGCCUGAGGGUGGG
|
|
1971
GAAGGGCCUGAGGGUGGGC
|
|
1972
AAGGGCCUGAGGGUGGGCA
|
|
1973
AGGGCCUGAGGGUGGGCAG
|
|
1974
GGGCCUGAGGGUGGGCAGU
|
|
1975
GGCCUGAGGGUGGGCAGUC
|
|
1976
GCCUGAGGGUGGGCAGUCU
|
|
1977
CCUGAGGGUGGGCAGUCUG
|
|
1978
CUGAGGGUGGGCAGUCUGU
|
|
1979
UGAGGGUGGGCAGUCUGUC
|
|
1980
GAGGGUGGGCAGUCUGUCU
|
|
1981
AGGGUGGGCAGUCUGUCUA
|
|
1982
GGGUGGGCAGUCUGUCUAU
|
|
1983
GGUGGGCAGUCUGUCUAUC
|
|
1984
GUGGGCAGUCUGUCUAUCA
|
|
1985
UGGGCAGUCUGUCUAUCAU
|
|
1986
GGGCAGUCUGUCUAUCAUG
|
|
1987
GGCAGUCUGUCUAUCAUGU
|
|
1988
GCAGUCUGUCUAUCAUGUA
|
|
1989
CAGUCUGUCUAUCAUGUAC
|
|
1990
AGUCUGUCUAUCAUGUACA
|
|
1991
GUCUGUCUAUCAUGUACAU
|
|
1992
UCUGUCUAUCAUGUACAUC
|
|
1993
CUGUCUAUCAUGUACAUCU
|
|
1994
UGUCUAUCAUGUACAUCUC
|
|
1995
GUCUAUCAUGUACAUCUCC
|
|
1996
UCUAUCAUGUACAUCUCCA
|
|
1997
CUAUCAUGUACAUCUCCAU
|
|
1998
UAUCAUGUACAUCUCCAUA
|
|
1999
AUCAUGUACAUCUCCAUAU
|
|
2000
UCAUGUACAUCUCCAUAUU
|
|
2001
CAUGUACAUCUCCAUAUUC
|
|
2002
AUGUACAUCUCCAUAUUCU
|
|
2003
UGUACAUCUCCAUAUUCUG
|
|
2004
GUACAUCUCCAUAUUCUGG
|
|
2005
UACAUCUCCAUAUUCUGGG
|
|
2006
ACAUCUCCAUAUUCUGGGA
|
|
2007
CAUCUCCAUAUUCUGGGAG
|
|
2008
AUCUCCAUAUUCUGGGAGG
|
|
2009
UCUCCAUAUUCUGGGAGGU
|
|
2010
CUCCAUAUUCUGGGAGGUC
|
|
2011
UCCAUAUUCUGGGAGGUCG
|
|
2012
CCAUAUUCUGGGAGGUCGU
|
|
2013
CAUAUUCUGGGAGGUCGUC
|
|
2014
AUAUUCUGGGAGGUCGUCA
|
|
2015
UAUUCUGGGAGGUCGUCAG
|
|
2016
AUUCUGGGAGGUCGUCAGU
|
|
2017
UUCUGGGAGGUCGUCAGUU
|
|
2018
UCUGGGAGGUCGUCAGUUG
|
|
2019
CUGGGAGGUCGUCAGUUGG
|
|
2020
UGGGAGGUCGUCAGUUGGG
|
|
2021
GGGAGGUCGUCAGUUGGGC
|
|
2022
GGAGGUCGUCAGUUGGGCU
|
|
2023
GAGGUCGUCAGUUGGGCUG
|
|
2024
AGGUCGUCAGUUGGGCUGG
|
|
2025
GGUCGUCAGUUGGGCUGGC
|
|
2026
GUCGUCAGUUGGGCUGGCC
|
|
2027
UCGUCAGUUGGGCUGGCCU
|
|
2028
CGUCAGUUGGGCUGGCCUC
|
|
2029
GUCAGUUGGGCUGGCCUCC
|
|
2030
UCAGUUGGGCUGGCCUCCU
|
|
2031
CAGUUGGGCUGGCCUCCUG
|
|
2032
AGUUGGGCUGGCCUCCUGG
|
|
2033
GUUGGGCUGGCCUCCUGGC
|
|
2034
UUGGGCUGGCCUCCUGGCU
|
|
2035
UGGGCUGGCCUCCUGGCUA
|
|
2036
GGGCUGGCCUCCUGGCUAA
|
|
2037
GGCUGGCCUCCUGGCUAAG
|
|
2038
GCUGGCCUCCUGGCUAAGA
|
|
2039
CUGGCCUCCUGGCUAAGAU
|
|
2040
UGGCCUCCUGGCUAAGAUU
|
|
2041
GGCCUCCUGGCUAAGAUUU
|
|
2042
GCCUCCUGGCUAAGAUUUU
|
|
2043
CCUCCUGGCUAAGAUUUUU
|
|
2044
CUCCUGGCUAAGAUUUUUG
|
|
2045
UCCUGGCUAAGAUUUUUGC
|
|
2046
CCUGGCUAAGAUUUUUGCA
|
|
2047
CUGGCUAAGAUUUUUGCAC
|
|
2048
UGGCUAAGAUUUUUGCACC
|
|
2049
GGCUAAGAUUUUUGCACCA
|
|
2050
GCUAAGAUUUUUGCACCAC
|
|
2051
CUAAGAUUUUUGCACCACA
|
|
2052
UAAGAUUUUUGCACCACAA
|
|
2053
AAGAUUUUUGCACCACAAG
|
|
2054
AGAUUUUUGCACCACAAGA
|
|
2055
GAUUUUUGCACCACAAGAG
|
|
2056
AUUUUUGCACCACAAGAGA
|
|
2057
UUUUUGCACCACAAGAGAU
|
|
2058
UUUUGCACCACAAGAGAUG
|
|
2059
UUUGCACCACAAGAGAUGC
|
|
2060
UUGCACCACAAGAGAUGCU
|
|
2061
UGCACCACAAGAGAUGCUG
|
|
2062
GCACCACAAGAGAUGCUGC
|
|
2063
CACCACAAGAGAUGCUGCA
|
|
2064
ACCACAAGAGAUGCUGCAU
|
|
2065
CCACAAGAGAUGCUGCAUG
|
|
2066
CACAAGAGAUGCUGCAUGU
|
|
2067
ACAAGAGAUGCUGCAUGUG
|
|
2068
CAAGAGAUGCUGCAUGUGU
|
|
2069
AAGAGAUGCUGCAUGUGUA
|
|
2070
AGAGAUGCUGCAUGUGUAC
|
|
2071
GAGAUGCUGCAUGUGUACA
|
|
2072
AGAUGCUGCAUGUGUACAA
|
|
2073
GAUGCUGCAUGUGUACAAA
|
|
2074
AUGCUGCAUGUGUACAAAU
|
|
2075
UGCUGCAUGUGUACAAAUC
|
|
2076
GCUGCAUGUGUACAAAUCA
|
|
2077
CUGCAUGUGUACAAAUCAC
|
|
2078
UGCAUGUGUACAAAUCACU
|
|
2079
GCAUGUGUACAAAUCACUA
|
|
2080
CAUGUGUACAAAUCACUAG
|
|
2081
AUGUGUACAAAUCACUAGC
|
|
2082
UGUGUACAAAUCACUAGCA
|
|
2083
GUGUACAAAUCACUAGCAA
|
|
2084
UGUACAAAUCACUAGCAAA
|
|
2085
GUACAAAUCACUAGCAAAU
|
|
2086
UACAAAUCACUAGCAAAUA
|
|
2087
ACAAAUCACUAGCAAAUAG
|
|
2088
CAAAUCACUAGCAAAUAGA
|
|
2089
AAAUCACUAGCAAAUAGAU
|
|
2090
AAUCACUAGCAAAUAGAUU
|
|
2091
AUCACUAGCAAAUAGAUUU
|
|
2092
UCACUAGCAAAUAGAUUUG
|
|
2093
CACUAGCAAAUAGAUUUGU
|
|
2094
ACUAGCAAAUAGAUUUGUU
|
|
2095
CUAGCAAAUAGAUUUGUUU
|
|
2096
UAGCAAAUAGAUUUGUUUC
|
|
2097
AGCAAAUAGAUUUGUUUCC
|
|
2098
GCAAAUAGAUUUGUUUCCC
|
|
2099
CAAAUAGAUUUGUUUCCCA
|
|
2100
AAAUAGAUUUGUUUCCCAU
|
|
2101
AAUAGAUUUGUUUCCCAUC
|
|
2102
AUAGAUUUGUUUCCCAUCA
|
|
2103
UAGAUUUGUUUCCCAUCAA
|
|
2104
AGAUUUGUUUCCCAUCAAC
|
|
2105
GAUUUGUUUCCCAUCAACU
|
|
2106
AUUUGUUUCCCAUCAACUU
|
|
2107
UUUGUUUCCCAUCAACUUA
|
|
2108
UUGUUUCCCAUCAACUUAG
|
|
2109
UGUUUCCCAUCAACUUAGC
|
|
2110
GUUUCCCAUCAACUUAGCC
|
|
2111
UUUCCCAUCAACUUAGCCA
|
|
2112
UUCCCAUCAACUUAGCCAC
|
|
2113
UCCCAUCAACUUAGCCACU
|
|
2114
CCCAUCAACUUAGCCACUG
|
|
2115
CCAUCAACUUAGCCACUGU
|
|
2116
CAUCAACUUAGCCACUGUU
|
|
2117
AUCAACUUAGCCACUGUUA
|
|
2118
UCAACUUAGCCACUGUUAA
|
|
2119
CAACUUAGCCACUGUUAAU
|
|
2120
AACUUAGCCACUGUUAAUG
|
|
2121
ACUUAGCCACUGUUAAUGU
|
|
2122
CUUAGCCACUGUUAAUGUA
|
|
2123
UUAGCCACUGUUAAUGUAA
|
|
2124
UAGCCACUGUUAAUGUAAA
|
|
2125
AGCCACUGUUAAUGUAAAU
|
|
2126
GCCACUGUUAAUGUAAAUU
|
|
2127
CCACUGUUAAUGUAAAUUG
|
|
2128
CACUGUUAAUGUAAAUUGU
|
|
2129
ACUGUUAAUGUAAAUUGUU
|
|
2130
CUGUUAAUGUAAAUUGUUC
|
|
2131
UGUUAAUGUAAAUUGUUCU
|
|
2132
GUUAAUGUAAAUUGUUCUU
|
|
2133
UUAAUGUAAAUUGUUCUUG
|
|
2134
UAAUGUAAAUUGUUCUUGG
|
|
2135
AAUGUAAAUUGUUCUUGGA
|
|
2136
AUGUAAAUUGUUCUUGGAU
|
|
2137
UGUAAAUUGUUCUUGGAUA
|
|
2138
GUAAAUUGUUCUUGGAUAU
|
|
2139
UAAAUUGUUCUUGGAUAUG
|
|
2140
AAAUUGUUCUUGGAUAUGU
|
|
2141
AAUUGUUCUUGGAUAUGUG
|
|
2142
AUUGUUCUUGGAUAUGUGU
|
|
2143
UUGUUCUUGGAUAUGUGUC
|
|
2144
UGUUCUUGGAUAUGUGUCU
|
|
2145
GUUCUUGGAUAUGUGUCUU
|
|
2146
UUCUUGGAUAUGUGUCUUU
|
|
2147
UCUUGGAUAUGUGUCUUUG
|
|
2148
CUUGGAUAUGUGUCUUUGG
|
|
2149
UUGGAUAUGUGUCUUUGGA
|
|
2150
UGGAUAUGUGUCUUUGGAG
|
|
2151
GGAUAUGUGUCUUUGGAGG
|
|
2152
GAUAUGUGUCUUUGGAGGG
|
|
2153
AUAUGUGUCUUUGGAGGGC
|
|
2154
UAUGUGUCUUUGGAGGGCA
|
|
2155
AUGUGUCUUUGGAGGGCAA
|
|
2156
UGUGUCUUUGGAGGGCAAU
|
|
2157
GUGUCUUUGGAGGGCAAUA
|
|
2158
UGUCUUUGGAGGGCAAUAA
|
|
2159
GUCUUUGGAGGGCAAUAAA
|
|
2160
UCUUUGGAGGGCAAUAAAU
|
|
2161
CUUUGGAGGGCAAUAAAUG
|
|
2162
UUUGGAGGGCAAUAAAUGC
|
|
2163
UUGGAGGGCAAUAAAUGCU
|
|
2164
UGGAGGGCAAUAAAUGCUC
|
|
2165
GGAGGGCAAUAAAUGCUCU
|
|
2166
GAGGGCAAUAAAUGCUCUG
|
|
2167
AGGGCAAUAAAUGCUCUGA
|
|
2168
GGGCAAUAAAUGCUCUGAA
|
|
2169
GGCAAUAAAUGCUCUGAAC
|
|
2170
GCAAUAAAUGCUCUGAACA
|
|
2171
CAAUAAAUGCUCUGAACAG
|
|
2172
AAUAAAUGCUCUGAACAGC
|
|
2173
AUAAAUGCUCUGAACAGCA
|
|
2174
UAAAUGCUCUGAACAGCAC
|
|
2175
AAAUGCUCUGAACAGCACU
|
|
2176
AAUGCUCUGAACAGCACUU
|
|
2177
AUGCUCUGAACAGCACUUG
|
|
2178
UGCUCUGAACAGCACUUGC
|
|
2179
GCUCUGAACAGCACUUGCA
|
|
2180
CUCUGAACAGCACUUGCAC
|
|
2181
UCUGAACAGCACUUGCACA
|
|
2182
CUGAACAGCACUUGCACAA
|
|
2183
UGAACAGCACUUGCACAAU
|
|
2184
GAACAGCACUUGCACAAUA
|
|
2185
CACUUGCACAAUAAAGAUA
|
|
2186
ACUUGCACAAUAAAGAUAC
|
|
2187
CUUGCACAAUAAAGAUACA
|
|
2188
UUGCACAAUAAAGAUACAG
|
|
2189
UGCACAAUAAAGAUACAGC
|
|
2190
GCACAAUAAAGAUACAGCA
|
|
2191
CACAAUAAAGAUACAGCAU
|
|
2192
ACAAUAAAGAUACAGCAUG
|
|
2193
CAAUAAAGAUACAGCAUGU
|
|
2194
AAUAAAGAUACAGCAUGUG
|
|
2195
AUAAAGAUACAGCAUGUGG
|
|
2196
UAAAGAUACAGCAUGUGGA
|
|
2197
AAAGAUACAGCAUGUGGAA
|
|
2198
AAGAUACAGCAUGUGGAAA
|
|
2199
AGAUACAGCAUGUGGAAAA
|
|
2200
GAUACAGCAUGUGGAAAAA
|
|
2201
AUACAGCAUGUGGAAAAAA
|
|
2202
UACAGCAUGUGGAAAAAAA
|
|
2203
ACAGCAUGUGGAAAAAAAA
|
|
2204
CAGCAUGUGGAAAAAAAAA
|
|
2205
AGCAUGUGGAAAAAAAAAA
|
|
2206
GCAUGUGGAAAAAAAAAAA
|
|
2207
CAUGUGGAAAAAAAAAAAA
|
|
2208
AUGUGGAAAAAAAAAAAAA
|
|
2209
UGUGGAAAAAAAAAAAAAA
|
|
TABLE 3
|
|
DsRNA molecules targeting mRNA encoding chicken
|
r-spondin.
|
SEQ ID NO
Sequence 5′-3′
|
|
2210
AUGGAUCUAACAGGCGGCA
|
|
2211
UGGAUCUAACAGGCGGCAG
|
|
2212
GGAUCUAACAGGCGGCAGC
|
|
2213
GAUCUAACAGGCGGCAGCA
|
|
2214
AUCUAACAGGCGGCAGCAA
|
|
2215
UCUAACAGGCGGCAGCAAA
|
|
2216
CUAACAGGCGGCAGCAAAG
|
|
2217
UAACAGGCGGCAGCAAAGU
|
|
2218
AACAGGCGGCAGCAAAGUG
|
|
2219
ACAGGCGGCAGCAAAGUGG
|
|
2220
CAGGCGGCAGCAAAGUGGU
|
|
2221
AGGCGGCAGCAAAGUGGUG
|
|
2222
GGCGGCAGCAAAGUGGUGA
|
|
2223
GCGGCAGCAAAGUGGUGAA
|
|
2224
CGGCAGCAAAGUGGUGAAG
|
|
2225
GGCAGCAAAGUGGUGAAGG
|
|
2226
GCAGCAAAGUGGUGAAGGG
|
|
2227
CAGCAAAGUGGUGAAGGGC
|
|
2228
AGCAAAGUGGUGAAGGGCA
|
|
2229
GCAAAGUGGUGAAGGGCAA
|
|
2230
CAAAGUGGUGAAGGGCAAG
|
|
2231
AAAGUGGUGAAGGGCAAGA
|
|
2232
AAGUGGUGAAGGGCAAGAG
|
|
2233
AGUGGUGAAGGGCAAGAGG
|
|
2234
GUGGUGAAGGGCAAGAGGC
|
|
2235
UGGUGAAGGGCAAGAGGCA
|
|
2236
GGUGAAGGGCAAGAGGCAA
|
|
2237
GUGAAGGGCAAGAGGCAAA
|
|
2238
UGAAGGGCAAGAGGCAAAG
|
|
2239
GAAGGGCAAGAGGCAAAGG
|
|
2240
AAGGGCAAGAGGCAAAGGC
|
|
2241
AGGGCAAGAGGCAAAGGCG
|
|
2242
GGGCAAGAGGCAAAGGCGA
|
|
2243
GGCAAGAGGCAAAGGCGAA
|
|
2244
GCAAGAGGCAAAGGCGAAU
|
|
2245
CAAGAGGCAAAGGCGAAUU
|
|
2246
AAGAGGCAAAGGCGAAUUA
|
|
2247
AGAGGCAAAGGCGAAUUAG
|
|
2248
GAGGCAAAGGCGAAUUAGC
|
|
2249
AGGCAAAGGCGAAUUAGCA
|
|
2250
GGCAAAGGCGAAUUAGCAC
|
|
2251
GCAAAGGCGAAUUAGCACU
|
|
2252
CAAAGGCGAAUUAGCACUG
|
|
2253
AAAGGCGAAUUAGCACUGA
|
|
2254
AAGGCGAAUUAGCACUGAG
|
|
2255
AGGCGAAUUAGCACUGAGC
|
|
2256
GGCGAAUUAGCACUGAGCU
|
|
2257
GCGAAUUAGCACUGAGCUG
|
|
2258
CGAAUUAGCACUGAGCUGA
|
|
2259
GAAUUAGCACUGAGCUGAG
|
|
2260
AAUUAGCACUGAGCUGAGC
|
|
2261
AUUAGCACUGAGCUGAGCC
|
|
2262
UUAGCACUGAGCUGAGCCA
|
|
2263
UAGCACUGAGCUGAGCCAG
|
|
2264
AGCACUGAGCUGAGCCAGG
|
|
2265
GCACUGAGCUGAGCCAGGG
|
|
2266
CACUGAGCUGAGCCAGGGC
|
|
2267
ACUGAGCUGAGCCAGGGCU
|
|
2268
CUGAGCUGAGCCAGGGCUG
|
|
2269
UGAGCUGAGCCAGGGCUGU
|
|
2270
GAGCUGAGCCAGGGCUGUG
|
|
2271
AGCUGAGCCAGGGCUGUGC
|
|
2272
GCUGAGCCAGGGCUGUGCC
|
|
2273
CUGAGCCAGGGCUGUGCCA
|
|
2274
UGAGCCAGGGCUGUGCCAG
|
|
2275
GAGCCAGGGCUGUGCCAGG
|
|
2276
AGCCAGGGCUGUGCCAGGG
|
|
2277
GCCAGGGCUGUGCCAGGGG
|
|
2278
CCAGGGCUGUGCCAGGGGC
|
|
2279
CAGGGCUGUGCCAGGGGCU
|
|
2280
AGGGCUGUGCCAGGGGCUG
|
|
2281
GGGCUGUGCCAGGGGCUGC
|
|
2282
GGCUGUGCCAGGGGCUGCG
|
|
2283
GCUGUGCCAGGGGCUGCGA
|
|
2284
CUGUGCCAGGGGCUGCGAC
|
|
2285
UGUGCCAGGGGCUGCGACC
|
|
2286
GUGCCAGGGGCUGCGACCU
|
|
2287
UGCCAGGGGCUGCGACCUG
|
|
2288
GCCAGGGGCUGCGACCUGU
|
|
2289
CCAGGGGCUGCGACCUGUG
|
|
2290
CAGGGGCUGCGACCUGUGC
|
|
2291
AGGGGCUGCGACCUGUGCU
|
|
2292
GGGGCUGCGACCUGUGCUC
|
|
2293
GGGCUGCGACCUGUGCUCU
|
|
2294
GGCUGCGACCUGUGCUCUG
|
|
2295
GCUGCGACCUGUGCUCUGA
|
|
2296
CUGCGACCUGUGCUCUGAG
|
|
2297
UGCGACCUGUGCUCUGAGU
|
|
2298
GCGACCUGUGCUCUGAGUU
|
|
2299
CGACCUGUGCUCUGAGUUC
|
|
2300
GACCUGUGCUCUGAGUUCA
|
|
2301
ACCUGUGCUCUGAGUUCAA
|
|
2302
CCUGUGCUCUGAGUUCAAC
|
|
2303
CUGUGCUCUGAGUUCAACG
|
|
2304
UGUGCUCUGAGUUCAACGG
|
|
2305
GUGCUCUGAGUUCAACGGG
|
|
2306
UGCUCUGAGUUCAACGGGU
|
|
2307
GCUCUGAGUUCAACGGGUG
|
|
2308
CUCUGAGUUCAACGGGUGC
|
|
2309
UCUGAGUUCAACGGGUGCC
|
|
2310
CUGAGUUCAACGGGUGCCU
|
|
2311
UGAGUUCAACGGGUGCCUG
|
|
2312
GAGUUCAACGGGUGCCUGA
|
|
2313
AGUUCAACGGGUGCCUGAG
|
|
2314
GUUCAACGGGUGCCUGAGA
|
|
2315
UUCAACGGGUGCCUGAGAU
|
|
2316
UCAACGGGUGCCUGAGAUG
|
|
2317
CAACGGGUGCCUGAGAUGU
|
|
2318
AACGGGUGCCUGAGAUGUU
|
|
2319
ACGGGUGCCUGAGAUGUUC
|
|
2320
CGGGUGCCUGAGAUGUUCC
|
|
2321
GGGUGCCUGAGAUGUUCCC
|
|
2322
GGUGCCUGAGAUGUUCCCC
|
|
2323
GUGCCUGAGAUGUUCCCCC
|
|
2324
UGCCUGAGAUGUUCCCCCA
|
|
2325
GCCUGAGAUGUUCCCCCAA
|
|
2326
CCUGAGAUGUUCCCCCAAG
|
|
2327
CUGAGAUGUUCCCCCAAGC
|
|
2328
UGAGAUGUUCCCCCAAGCU
|
|
2329
GAGAUGUUCCCCCAAGCUC
|
|
2330
AGAUGUUCCCCCAAGCUCU
|
|
2331
GAUGUUCCCCCAAGCUCUU
|
|
2332
AUGUUCCCCCAAGCUCUUC
|
|
2333
UGUUCCCCCAAGCUCUUCA
|
|
2334
GUUCCCCCAAGCUCUUCAU
|
|
2335
UUCCCCCAAGCUCUUCAUC
|
|
2336
UCCCCCAAGCUCUUCAUCC
|
|
2337
CCCCCAAGCUCUUCAUCCU
|
|
2338
CCCCAAGCUCUUCAUCCUU
|
|
2339
CCCAAGCUCUUCAUCCUUC
|
|
2340
CCAAGCUCUUCAUCCUUCU
|
|
2341
CAAGCUCUUCAUCCUUCUG
|
|
2342
AAGCUCUUCAUCCUUCUGG
|
|
2343
AGCUCUUCAUCCUUCUGGA
|
|
2344
GCUCUUCAUCCUUCUGGAG
|
|
2345
CUCUUCAUCCUUCUGGAGA
|
|
2346
UCUUCAUCCUUCUGGAGAG
|
|
2347
CUUCAUCCUUCUGGAGAGG
|
|
2348
UUCAUCCUUCUGGAGAGGA
|
|
2349
UCAUCCUUCUGGAGAGGAA
|
|
2350
CAUCCUUCUGGAGAGGAAC
|
|
2351
AUCCUUCUGGAGAGGAACG
|
|
2352
UCCUUCUGGAGAGGAACGA
|
|
2353
CCUUCUGGAGAGGAACGAU
|
|
2354
CUUCUGGAGAGGAACGAUA
|
|
2355
UUCUGGAGAGGAACGAUAU
|
|
2356
UCUGGAGAGGAACGAUAUC
|
|
2357
CUGGAGAGGAACGAUAUCC
|
|
2358
UGGAGAGGAACGAUAUCCG
|
|
2359
GGAGAGGAACGAUAUCCGG
|
|
2360
GAGAGGAACGAUAUCCGGC
|
|
2361
AGAGGAACGAUAUCCGGCA
|
|
2362
GAGGAACGAUAUCCGGCAA
|
|
2363
AGGAACGAUAUCCGGCAAA
|
|
2364
GGAACGAUAUCCGGCAAAU
|
|
2365
GAACGAUAUCCGGCAAAUU
|
|
2366
AACGAUAUCCGGCAAAUUG
|
|
2367
ACGAUAUCCGGCAAAUUGG
|
|
2368
CGAUAUCCGGCAAAUUGGG
|
|
2369
GAUAUCCGGCAAAUUGGGA
|
|
2370
AUAUCCGGCAAAUUGGGAU
|
|
2371
UAUCCGGCAAAUUGGGAUC
|
|
2372
AUCCGGCAAAUUGGGAUCU
|
|
2373
UCCGGCAAAUUGGGAUCUG
|
|
2374
CCGGCAAAUUGGGAUCUGC
|
|
2375
CGGCAAAUUGGGAUCUGCC
|
|
2376
GGCAAAUUGGGAUCUGCCU
|
|
2377
GCAAAUUGGGAUCUGCCUC
|
|
2378
CAAAUUGGGAUCUGCCUCC
|
|
2379
AAAUUGGGAUCUGCCUCCC
|
|
2380
AAUUGGGAUCUGCCUCCCA
|
|
2381
AUUGGGAUCUGCCUCCCAU
|
|
2382
UUGGGAUCUGCCUCCCAUC
|
|
2383
UGGGAUCUGCCUCCCAUCC
|
|
2384
GGGAUCUGCCUCCCAUCCU
|
|
2385
GGAUCUGCCUCCCAUCCUG
|
|
2386
GAUCUGCCUCCCAUCCUGU
|
|
2387
AUCUGCCUCCCAUCCUGUC
|
|
2388
UCUGCCUCCCAUCCUGUCC
|
|
2389
CUGCCUCCCAUCCUGUCCA
|
|
2390
UGCCUCCCAUCCUGUCCAC
|
|
2391
GCCUCCCAUCCUGUCCACU
|
|
2392
CCUCCCAUCCUGUCCACUG
|
|
2393
CUCCCAUCCUGUCCACUGG
|
|
2394
UCCCAUCCUGUCCACUGGG
|
|
2395
CCCAUCCUGUCCACUGGGA
|
|
2396
CCAUCCUGUCCACUGGGAU
|
|
2397
CAUCCUGUCCACUGGGAUA
|
|
2398
AUCCUGUCCACUGGGAUAC
|
|
2399
UCCUGUCCACUGGGAUACU
|
|
2400
CCUGUCCACUGGGAUACUU
|
|
2401
CUGUCCACUGGGAUACUUU
|
|
2402
UGUCCACUGGGAUACUUUG
|
|
2403
GUCCACUGGGAUACUUUGG
|
|
2404
UCCACUGGGAUACUUUGGC
|
|
2405
CCACUGGGAUACUUUGGCC
|
|
2406
CACUGGGAUACUUUGGCCU
|
|
2407
ACUGGGAUACUUUGGCCUU
|
|
2408
CUGGGAUACUUUGGCCUUC
|
|
2409
UGGGAUACUUUGGCCUUCG
|
|
2410
GGGAUACUUUGGCCUUCGC
|
|
2411
GGAUACUUUGGCCUUCGCA
|
|
2412
GAUACUUUGGCCUUCGCAA
|
|
2413
AUACUUUGGCCUUCGCAAU
|
|
2414
UACUUUGGCCUUCGCAAUA
|
|
2415
ACUUUGGCCUUCGCAAUAC
|
|
2416
CUUUGGCCUUCGCAAUACA
|
|
2417
UUUGGCCUUCGCAAUACAG
|
|
2418
UUGGCCUUCGCAAUACAGA
|
|
2419
UGGCCUUCGCAAUACAGAC
|
|
2420
GGCCUUCGCAAUACAGACA
|
|
2421
GCCUUCGCAAUACAGACAU
|
|
2422
CCUUCGCAAUACAGACAUG
|
|
2423
CUUCGCAAUACAGACAUGA
|
|
2424
UUCGCAAUACAGACAUGAA
|
|
2425
UCGCAAUACAGACAUGAAC
|
|
2426
CGCAAUACAGACAUGAACA
|
|
2427
GCAAUACAGACAUGAACAA
|
|
2428
CAAUACAGACAUGAACAAG
|
|
2429
AAUACAGACAUGAACAAGU
|
|
2430
AUACAGACAUGAACAAGUG
|
|
2431
UACAGACAUGAACAAGUGC
|
|
2432
ACAGACAUGAACAAGUGCA
|
|
2433
CAGACAUGAACAAGUGCAU
|
|
2434
AGACAUGAACAAGUGCAUC
|
|
2435
GACAUGAACAAGUGCAUCA
|
|
2436
ACAUGAACAAGUGCAUCAA
|
|
2437
CAUGAACAAGUGCAUCAAA
|
|
2438
AUGAACAAGUGCAUCAAAU
|
|
2439
UGAACAAGUGCAUCAAAUG
|
|
2440
GAACAAGUGCAUCAAAUGC
|
|
2441
AACAAGUGCAUCAAAUGCA
|
|
2442
ACAAGUGCAUCAAAUGCAA
|
|
2443
CAAGUGCAUCAAAUGCAAA
|
|
2444
AAGUGCAUCAAAUGCAAAA
|
|
2445
AGUGCAUCAAAUGCAAAAU
|
|
2446
GUGCAUCAAAUGCAAAAUC
|
|
2447
UGCAUCAAAUGCAAAAUCG
|
|
2448
GCAUCAAAUGCAAAAUCGA
|
|
2449
CAUCAAAUGCAAAAUCGAG
|
|
2450
AUCAAAUGCAAAAUCGAGA
|
|
2451
UCAAAUGCAAAAUCGAGAA
|
|
2452
CAAAUGCAAAAUCGAGAAC
|
|
2453
AAAUGCAAAAUCGAGAACU
|
|
2454
AAUGCAAAAUCGAGAACUG
|
|
2455
AUGCAAAAUCGAGAACUGU
|
|
2456
UGCAAAAUCGAGAACUGUG
|
|
2457
GCAAAAUCGAGAACUGUGA
|
|
2458
CAAAAUCGAGAACUGUGAG
|
|
2459
AAAAUCGAGAACUGUGAGU
|
|
2460
AAAUCGAGAACUGUGAGUC
|
|
2461
AAUCGAGAACUGUGAGUCC
|
|
2462
AUCGAGAACUGUGAGUCCU
|
|
2463
UCGAGAACUGUGAGUCCUG
|
|
2464
CGAGAACUGUGAGUCCUGC
|
|
2465
GAGAACUGUGAGUCCUGCU
|
|
2466
AGAACUGUGAGUCCUGCUU
|
|
2467
GAACUGUGAGUCCUGCUUC
|
|
2468
AACUGUGAGUCCUGCUUCA
|
|
2469
ACUGUGAGUCCUGCUUCAG
|
|
2470
CUGUGAGUCCUGCUUCAGC
|
|
2471
UGUGAGUCCUGCUUCAGCC
|
|
2472
GUGAGUCCUGCUUCAGCCG
|
|
2473
UGAGUCCUGCUUCAGCCGA
|
|
2474
GAGUCCUGCUUCAGCCGAA
|
|
2475
AGUCCUGCUUCAGCCGAAA
|
|
2476
GUCCUGCUUCAGCCGAAAC
|
|
2477
UCCUGCUUCAGCCGAAACU
|
|
2478
CCUGCUUCAGCCGAAACUU
|
|
2479
CUGCUUCAGCCGAAACUUU
|
|
2480
UGCUUCAGCCGAAACUUUU
|
|
2481
GCUUCAGCCGAAACUUUUG
|
|
2482
CUUCAGCCGAAACUUUUGC
|
|
2483
UUCAGCCGAAACUUUUGCA
|
|
2484
UCAGCCGAAACUUUUGCAC
|
|
2485
CAGCCGAAACUUUUGCACA
|
|
2486
AGCCGAAACUUUUGCACAA
|
|
2487
GCCGAAACUUUUGCACAAA
|
|
2488
CCGAAACUUUUGCACAAAA
|
|
2489
CGAAACUUUUGCACAAAAU
|
|
2490
GAAACUUUUGCACAAAAUG
|
|
2491
AAACUUUUGCACAAAAUGU
|
|
2492
AACUUUUGCACAAAAUGUA
|
|
2493
ACUUUUGCACAAAAUGUAA
|
|
2494
CUUUUGCACAAAAUGUAAG
|
|
2495
UUUUGCACAAAAUGUAAGG
|
|
2496
UUUGCACAAAAUGUAAGGA
|
|
2497
UUGCACAAAAUGUAAGGAA
|
|
2498
UGCACAAAAUGUAAGGAAG
|
|
2499
GCACAAAAUGUAAGGAAGG
|
|
2500
CACAAAAUGUAAGGAAGGU
|
|
2501
ACAAAAUGUAAGGAAGGUU
|
|
2502
CAAAAUGUAAGGAAGGUUU
|
|
2503
AAAAUGUAAGGAAGGUUUG
|
|
2504
AAAUGUAAGGAAGGUUUGU
|
|
2505
AAUGUAAGGAAGGUUUGUA
|
|
2506
AUGUAAGGAAGGUUUGUAU
|
|
2507
UGUAAGGAAGGUUUGUAUU
|
|
2508
GUAAGGAAGGUUUGUAUUU
|
|
2509
UAAGGAAGGUUUGUAUUUG
|
|
2510
AAGGAAGGUUUGUAUUUGC
|
|
2511
AGGAAGGUUUGUAUUUGCA
|
|
2512
GGAAGGUUUGUAUUUGCAC
|
|
2513
GAAGGUUUGUAUUUGCACA
|
|
2514
AAGGUUUGUAUUUGCACAA
|
|
2515
AGGUUUGUAUUUGCACAAA
|
|
2516
GGUUUGUAUUUGCACAAAG
|
|
2517
GUUUGUAUUUGCACAAAGG
|
|
2518
UUUGUAUUUGCACAAAGGG
|
|
2519
UUGUAUUUGCACAAAGGGA
|
|
2520
UGUAUUUGCACAAAGGGAG
|
|
2521
GUAUUUGCACAAAGGGAGA
|
|
2522
UAUUUGCACAAAGGGAGAU
|
|
2523
AUUUGCACAAAGGGAGAUG
|
|
2524
UUUGCACAAAGGGAGAUGU
|
|
2525
UUGCACAAAGGGAGAUGUU
|
|
2526
UGCACAAAGGGAGAUGUUA
|
|
2527
GCACAAAGGGAGAUGUUAC
|
|
2528
CACAAAGGGAGAUGUUACG
|
|
2529
ACAAAGGGAGAUGUUACGU
|
|
2530
CAAAGGGAGAUGUUACGUC
|
|
2531
AAAGGGAGAUGUUACGUCA
|
|
2532
AAGGGAGAUGUUACGUCAC
|
|
2533
AGGGAGAUGUUACGUCACG
|
|
2534
GGGAGAUGUUACGUCACGU
|
|
2535
GGAGAUGUUACGUCACGUG
|
|
2536
GAGAUGUUACGUCACGUGC
|
|
2537
AGAUGUUACGUCACGUGCC
|
|
2538
GAUGUUACGUCACGUGCCC
|
|
2539
AUGUUACGUCACGUGCCCC
|
|
2540
UGUUACGUCACGUGCCCCG
|
|
2541
GUUACGUCACGUGCCCCGA
|
|
2542
UUACGUCACGUGCCCCGAA
|
|
2543
UACGUCACGUGCCCCGAAG
|
|
2544
ACGUCACGUGCCCCGAAGG
|
|
2545
CGUCACGUGCCCCGAAGGC
|
|
2546
GUCACGUGCCCCGAAGGCU
|
|
2547
UCACGUGCCCCGAAGGCUA
|
|
2548
CACGUGCCCCGAAGGCUAC
|
|
2549
ACGUGCCCCGAAGGCUACU
|
|
2550
CGUGCCCCGAAGGCUACUC
|
|
2551
GUGCCCCGAAGGCUACUCU
|
|
2552
UGCCCCGAAGGCUACUCUG
|
|
2553
GCCCCGAAGGCUACUCUGC
|
|
2554
CCCCGAAGGCUACUCUGCU
|
|
2555
CCCGAAGGCUACUCUGCUG
|
|
2556
CCGAAGGCUACUCUGCUGC
|
|
2557
CGAAGGCUACUCUGCUGCC
|
|
2558
GAAGGCUACUCUGCUGCCA
|
|
2559
AAGGCUACUCUGCUGCCAA
|
|
2560
AGGCUACUCUGCUGCCAAU
|
|
2561
GGCUACUCUGCUGCCAAUG
|
|
2562
GCUACUCUGCUGCCAAUGG
|
|
2563
CUACUCUGCUGCCAAUGGC
|
|
2564
UACUCUGCUGCCAAUGGCA
|
|
2565
ACUCUGCUGCCAAUGGCAC
|
|
2566
CUCUGCUGCCAAUGGCACC
|
|
2567
UCUGCUGCCAAUGGCACCA
|
|
2568
CUGCUGCCAAUGGCACCAU
|
|
2569
UGCUGCCAAUGGCACCAUG
|
|
2570
GCUGCCAAUGGCACCAUGG
|
|
2571
CUGCCAAUGGCACCAUGGA
|
|
2572
UGCCAAUGGCACCAUGGAG
|
|
2573
GCCAAUGGCACCAUGGAGU
|
|
2574
CCAAUGGCACCAUGGAGUG
|
|
2575
CAAUGGCACCAUGGAGUGC
|
|
2576
AAUGGCACCAUGGAGUGCA
|
|
2577
AUGGCACCAUGGAGUGCAG
|
|
2578
UGGCACCAUGGAGUGCAGC
|
|
2579
GGCACCAUGGAGUGCAGCA
|
|
2580
GCACCAUGGAGUGCAGCAG
|
|
2581
CACCAUGGAGUGCAGCAGU
|
|
2582
ACCAUGGAGUGCAGCAGUC
|
|
2583
CCAUGGAGUGCAGCAGUCC
|
|
2584
CAUGGAGUGCAGCAGUCCU
|
|
2585
AUGGAGUGCAGCAGUCCUG
|
|
2586
UGGAGUGCAGCAGUCCUGC
|
|
2587
GGAGUGCAGCAGUCCUGCG
|
|
2588
GAGUGCAGCAGUCCUGCGC
|
|
2589
AGUGCAGCAGUCCUGCGCA
|
|
2590
GUGCAGCAGUCCUGCGCAA
|
|
2591
UGCAGCAGUCCUGCGCAAU
|
|
2592
GCAGCAGUCCUGCGCAAUG
|
|
2593
CAGCAGUCCUGCGCAAUGU
|
|
2594
AGCAGUCCUGCGCAAUGUG
|
|
2595
GCAGUCCUGCGCAAUGUGA
|
|
2596
CAGUCCUGCGCAAUGUGAA
|
|
2597
AGUCCUGCGCAAUGUGAAA
|
|
2598
GUCCUGCGCAAUGUGAAAU
|
|
2599
UCCUGCGCAAUGUGAAAUG
|
|
2600
CCUGCGCAAUGUGAAAUGA
|
|
2601
CUGCGCAAUGUGAAAUGAG
|
|
2602
UGCGCAAUGUGAAAUGAGU
|
|
2603
GCGCAAUGUGAAAUGAGUG
|
|
2604
CGCAAUGUGAAAUGAGUGA
|
|
2605
GCAAUGUGAAAUGAGUGAG
|
|
2606
CAAUGUGAAAUGAGUGAGU
|
|
2607
AAUGUGAAAUGAGUGAGUG
|
|
2608
AUGUGAAAUGAGUGAGUGG
|
|
2609
UGUGAAAUGAGUGAGUGGG
|
|
2610
GUGAAAUGAGUGAGUGGGG
|
|
2611
UGAAAUGAGUGAGUGGGGG
|
|
2612
GAAAUGAGUGAGUGGGGGC
|
|
2613
AAAUGAGUGAGUGGGGGCC
|
|
2614
AAUGAGUGAGUGGGGGCCC
|
|
2615
AUGAGUGAGUGGGGGCCCU
|
|
2616
UGAGUGAGUGGGGGCCCUG
|
|
2617
GAGUGAGUGGGGGCCCUGG
|
|
2618
AGUGAGUGGGGGCCCUGGG
|
|
2619
GUGAGUGGGGGCCCUGGGG
|
|
2620
UGAGUGGGGGCCCUGGGGG
|
|
2621
GAGUGGGGGCCCUGGGGGC
|
|
2622
AGUGGGGGCCCUGGGGGCC
|
|
2623
GUGGGGGCCCUGGGGGCCC
|
|
2624
UGGGGGCCCUGGGGGCCCU
|
|
2625
GGGGGCCCUGGGGGCCCUG
|
|
2626
GGGGCCCUGGGGGCCCUGC
|
|
2627
GGGCCCUGGGGGCCCUGCU
|
|
2628
GGCCCUGGGGGCCCUGCUC
|
|
2629
GCCCUGGGGGCCCUGCUCC
|
|
2630
CCCUGGGGGCCCUGCUCCA
|
|
2631
CCUGGGGGCCCUGCUCCAA
|
|
2632
CUGGGGGCCCUGCUCCAAG
|
|
2633
UGGGGGCCCUGCUCCAAGA
|
|
2634
GGGGGCCCUGCUCCAAGAA
|
|
2635
GGGGCCCUGCUCCAAGAAG
|
|
2636
GGGCCCUGCUCCAAGAAGA
|
|
2637
GGCCCUGCUCCAAGAAGAG
|
|
2638
GCCCUGCUCCAAGAAGAGG
|
|
2639
CCCUGCUCCAAGAAGAGGA
|
|
2640
CCUGCUCCAAGAAGAGGAA
|
|
2641
CUGCUCCAAGAAGAGGAAG
|
|
2642
UGCUCCAAGAAGAGGAAGC
|
|
2643
GCUCCAAGAAGAGGAAGCU
|
|
2644
CUCCAAGAAGAGGAAGCUG
|
|
2645
UCCAAGAAGAGGAAGCUGU
|
|
2646
CCAAGAAGAGGAAGCUGUG
|
|
2647
CAAGAAGAGGAAGCUGUGU
|
|
2648
AAGAAGAGGAAGCUGUGUG
|
|
2649
AGAAGAGGAAGCUGUGUGG
|
|
2650
GAAGAGGAAGCUGUGUGGC
|
|
2651
AAGAGGAAGCUGUGUGGCU
|
|
2652
AGAGGAAGCUGUGUGGCUU
|
|
2653
GAGGAAGCUGUGUGGCUUC
|
|
2654
AGGAAGCUGUGUGGCUUCA
|
|
2655
GGAAGCUGUGUGGCUUCAA
|
|
2656
GAAGCUGUGUGGCUUCAAG
|
|
2657
AAGCUGUGUGGCUUCAAGA
|
|
2658
AGCUGUGUGGCUUCAAGAA
|
|
2659
GCUGUGUGGCUUCAAGAAG
|
|
2660
CUGUGUGGCUUCAAGAAGG
|
|
2661
UGUGUGGCUUCAAGAAGGG
|
|
2662
GUGUGGCUUCAAGAAGGGG
|
|
2663
UGUGGCUUCAAGAAGGGGA
|
|
2664
GUGGCUUCAAGAAGGGGAA
|
|
2665
UGGCUUCAAGAAGGGGAAC
|
|
2666
GGCUUCAAGAAGGGGAACG
|
|
2667
GCUUCAAGAAGGGGAACGA
|
|
2668
CUUCAAGAAGGGGAACGAG
|
|
2669
UUCAAGAAGGGGAACGAGG
|
|
2670
UCAAGAAGGGGAACGAGGA
|
|
2671
CAAGAAGGGGAACGAGGAC
|
|
2672
AAGAAGGGGAACGAGGACC
|
|
2673
AGAAGGGGAACGAGGACCG
|
|
2674
GAAGGGGAACGAGGACCGA
|
|
2675
AAGGGGAACGAGGACCGAA
|
|
2676
AGGGGAACGAGGACCGAAC
|
|
2677
GGGGAACGAGGACCGAACG
|
|
2678
GGGAACGAGGACCGAACGC
|
|
2679
GGAACGAGGACCGAACGCG
|
|
2680
GAACGAGGACCGAACGCGG
|
|
2681
AACGAGGACCGAACGCGGC
|
|
2682
ACGAGGACCGAACGCGGCG
|
|
2683
CGAGGACCGAACGCGGCGG
|
|
2684
GAGGACCGAACGCGGCGGA
|
|
2685
AGGACCGAACGCGGCGGAU
|
|
2686
GGACCGAACGCGGCGGAUC
|
|
2687
GACCGAACGCGGCGGAUCC
|
|
2688
ACCGAACGCGGCGGAUCCU
|
|
2689
CCGAACGCGGCGGAUCCUG
|
|
2690
CGAACGCGGCGGAUCCUGC
|
|
2691
GAACGCGGCGGAUCCUGCA
|
|
2692
AACGCGGCGGAUCCUGCAG
|
|
2693
ACGCGGCGGAUCCUGCAGG
|
|
2694
CGCGGCGGAUCCUGCAGGC
|
|
2695
GCGGCGGAUCCUGCAGGCU
|
|
2696
CGGCGGAUCCUGCAGGCUC
|
|
2697
GGCGGAUCCUGCAGGCUCC
|
|
2698
GCGGAUCCUGCAGGCUCCC
|
|
2699
CGGAUCCUGCAGGCUCCCU
|
|
2700
GGAUCCUGCAGGCUCCCUC
|
|
2701
GAUCCUGCAGGCUCCCUCU
|
|
2702
AUCCUGCAGGCUCCCUCUG
|
|
2703
UCCUGCAGGCUCCCUCUGG
|
|
2704
CCUGCAGGCUCCCUCUGGG
|
|
2705
CUGCAGGCUCCCUCUGGGG
|
|
2706
UGCAGGCUCCCUCUGGGGA
|
|
2707
GCAGGCUCCCUCUGGGGAC
|
|
2708
CAGGCUCCCUCUGGGGACG
|
|
2709
AGGCUCCCUCUGGGGACGU
|
|
2710
GGCUCCCUCUGGGGACGUG
|
|
2711
GCUCCCUCUGGGGACGUGU
|
|
2712
CUCCCUCUGGGGACGUGUC
|
|
2713
UCCCUCUGGGGACGUGUCC
|
|
2714
CCCUCUGGGGACGUGUCCC
|
|
2715
CCUCUGGGGACGUGUCCCU
|
|
2716
CUCUGGGGACGUGUCCCUG
|
|
2717
UCUGGGGACGUGUCCCUGU
|
|
2718
CUGGGGACGUGUCCCUGUG
|
|
2719
UGGGGACGUGUCCCUGUGC
|
|
2720
GGGGACGUGUCCCUGUGCC
|
|
2721
GGGACGUGUCCCUGUGCCC
|
|
2722
GGACGUGUCCCUGUGCCCC
|
|
2723
GACGUGUCCCUGUGCCCCG
|
|
2724
ACGUGUCCCUGUGCCCCGC
|
|
2725
CGUGUCCCUGUGCCCCGCC
|
|
2726
GUGUCCCUGUGCCCCGCCA
|
|
2727
UGUCCCUGUGCCCCGCCAC
|
|
2728
GUCCCUGUGCCCCGCCACC
|
|
2729
UCCCUGUGCCCCGCCACCA
|
|
2730
CCCUGUGCCCCGCCACCAC
|
|
2731
CCUGUGCCCCGCCACCACG
|
|
2732
CUGUGCCCCGCCACCACGG
|
|
2733
UGUGCCCCGCCACCACGGA
|
|
2734
GUGCCCCGCCACCACGGAG
|
|
2735
UGCCCCGCCACCACGGAGG
|
|
2736
GCCCCGCCACCACGGAGGU
|
|
2737
CCCCGCCACCACGGAGGUG
|
|
2738
CCCGCCACCACGGAGGUGC
|
|
2739
CCGCCACCACGGAGGUGCG
|
|
2740
CGCCACCACGGAGGUGCGC
|
|
2741
GCCACCACGGAGGUGCGCA
|
|
2742
CCACCACGGAGGUGCGCAG
|
|
2743
CACCACGGAGGUGCGCAGA
|
|
2744
ACCACGGAGGUGCGCAGAU
|
|
2745
CCACGGAGGUGCGCAGAUG
|
|
2746
CACGGAGGUGCGCAGAUGC
|
|
2747
ACGGAGGUGCGCAGAUGCA
|
|
2748
CGGAGGUGCGCAGAUGCAC
|
|
2749
GGAGGUGCGCAGAUGCACU
|
|
2750
GAGGUGCGCAGAUGCACUG
|
|
2751
AGGUGCGCAGAUGCACUGU
|
|
2752
GGUGCGCAGAUGCACUGUG
|
|
2753
GUGCGCAGAUGCACUGUGC
|
|
2754
UGCGCAGAUGCACUGUGCA
|
|
2755
GCGCAGAUGCACUGUGCAG
|
|
2756
CGCAGAUGCACUGUGCAGA
|
|
2757
GCAGAUGCACUGUGCAGAA
|
|
2758
CAGAUGCACUGUGCAGAAG
|
|
2759
AGAUGCACUGUGCAGAAGA
|
|
2760
GAUGCACUGUGCAGAAGAG
|
|
2761
AUGCACUGUGCAGAAGAGC
|
|
2762
UGCACUGUGCAGAAGAGCC
|
|
2763
GCACUGUGCAGAAGAGCCA
|
|
2764
CACUGUGCAGAAGAGCCAA
|
|
2765
ACUGUGCAGAAGAGCCAAU
|
|
2766
CUGUGCAGAAGAGCCAAUG
|
|
2767
UGUGCAGAAGAGCCAAUGC
|
|
2768
GUGCAGAAGAGCCAAUGCC
|
|
2769
UGCAGAAGAGCCAAUGCCC
|
|
2770
GCAGAAGAGCCAAUGCCCC
|
|
2771
CAGAAGAGCCAAUGCCCCG
|
|
2772
AGAAGAGCCAAUGCCCCGA
|
|
2773
GAAGAGCCAAUGCCCCGAA
|
|
2774
AAGAGCCAAUGCCCCGAAG
|
|
2775
AGAGCCAAUGCCCCGAAGG
|
|
2776
GAGCCAAUGCCCCGAAGGG
|
|
2777
AGCCAAUGCCCCGAAGGGA
|
|
2778
GCCAAUGCCCCGAAGGGAA
|
|
2779
CCAAUGCCCCGAAGGGAAA
|
|
2780
CAAUGCCCCGAAGGGAAAA
|
|
2781
AAUGCCCCGAAGGGAAAAG
|
|
2782
AUGCCCCGAAGGGAAAAGG
|
|
2783
UGCCCCGAAGGGAAAAGGA
|
|
2784
GCCCCGAAGGGAAAAGGAA
|
|
2785
CCCCGAAGGGAAAAGGAAG
|
|
2786
CCCGAAGGGAAAAGGAAGA
|
|
2787
CCGAAGGGAAAAGGAAGAA
|
|
2788
CGAAGGGAAAAGGAAGAAA
|
|
2789
GAAGGGAAAAGGAAGAAAA
|
|
2790
AAGGGAAAAGGAAGAAAAA
|
|
2791
AGGGAAAAGGAAGAAAAAG
|
|
2792
GGGAAAAGGAAGAAAAAGG
|
|
2793
GGAAAAGGAAGAAAAAGGA
|
|
2794
GAAAAGGAAGAAAAAGGAC
|
|
2795
AAAAGGAAGAAAAAGGACG
|
|
2796
AAAGGAAGAAAAAGGACGA
|
|
2797
AAGGAAGAAAAAGGACGAG
|
|
2798
AGGAAGAAAAAGGACGAGC
|
|
2799
GGAAGAAAAAGGACGAGCA
|
|
2800
GAAGAAAAAGGACGAGCAA
|
|
2801
AAGAAAAAGGACGAGCAAG
|
|
2802
AGAAAAAGGACGAGCAAGG
|
|
2803
GAAAAAGGACGAGCAAGGA
|
|
2804
AAAAAGGACGAGCAAGGAA
|
|
2805
AAAAGGACGAGCAAGGAAA
|
|
2806
AAAGGACGAGCAAGGAAAG
|
|
2807
AAGGACGAGCAAGGAAAGC
|
|
2808
AGGACGAGCAAGGAAAGCA
|
|
2809
GGACGAGCAAGGAAAGCAA
|
|
2810
GACGAGCAAGGAAAGCAAG
|
|
2811
ACGAGCAAGGAAAGCAAGA
|
|
2812
CGAGCAAGGAAAGCAAGAU
|
|
2813
GAGCAAGGAAAGCAAGAUA
|
|
2814
AGCAAGGAAAGCAAGAUAA
|
|
2815
GCAAGGAAAGCAAGAUAAU
|
|
2816
CAAGGAAAGCAAGAUAAUA
|
|
2817
AAGGAAAGCAAGAUAAUAC
|
|
2818
AGGAAAGCAAGAUAAUACA
|
|
2819
GGAAAGCAAGAUAAUACAA
|
|
2820
GAAAGCAAGAUAAUACAAA
|
|
2821
AAAGCAAGAUAAUACAAAC
|
|
2822
AAGCAAGAUAAUACAAACG
|
|
2823
AGCAAGAUAAUACAAACGG
|
|
2824
GCAAGAUAAUACAAACGGG
|
|
2825
CAAGAUAAUACAAACGGGA
|
|
2826
AAGAUAAUACAAACGGGAA
|
|
2827
AGAUAAUACAAACGGGAAC
|
|
2828
GAUAAUACAAACGGGAACA
|
|
2829
AUAAUACAAACGGGAACAG
|
|
2830
UAAUACAAACGGGAACAGA
|
|
2831
AAUACAAACGGGAACAGAA
|
|
2832
AUACAAACGGGAACAGAAA
|
|
2833
UACAAACGGGAACAGAAAU
|
|
2834
ACAAACGGGAACAGAAAUC
|
|
2835
CAAACGGGAACAGAAAUCG
|
|
2836
AAACGGGAACAGAAAUCGG
|
|
2837
AACGGGAACAGAAAUCGGA
|
|
2838
ACGGGAACAGAAAUCGGAA
|
|
2839
CGGGAACAGAAAUCGGAAA
|
|
2840
GGGAACAGAAAUCGGAAAG
|
|
2841
GGAACAGAAAUCGGAAAGA
|
|
2842
GAACAGAAAUCGGAAAGAC
|
|
2843
AACAGAAAUCGGAAAGACA
|
|
2844
ACAGAAAUCGGAAAGACAC
|
|
2845
CAGAAAUCGGAAAGACACC
|
|
2846
AGAAAUCGGAAAGACACCA
|
|
2847
GAAAUCGGAAAGACACCAA
|
|
2848
AAAUCGGAAAGACACCAAA
|
|
2849
AAUCGGAAAGACACCAAAG
|
|
2850
AUCGGAAAGACACCAAAGA
|
|
2851
UCGGAAAGACACCAAAGAU
|
|
2852
CGGAAAGACACCAAAGAUG
|
|
2853
GGAAAGACACCAAAGAUGC
|
|
2854
GAAAGACACCAAAGAUGCA
|
|
2855
AAAGACACCAAAGAUGCAA
|
|
2856
AAGACACCAAAGAUGCAAA
|
|
2857
AGACACCAAAGAUGCAAAG
|
|
2858
GACACCAAAGAUGCAAAGU
|
|
2859
ACACCAAAGAUGCAAAGUC
|
|
2860
CACCAAAGAUGCAAAGUCU
|
|
2861
ACCAAAGAUGCAAAGUCUG
|
|
2862
CCAAAGAUGCAAAGUCUGG
|
|
2863
CAAAGAUGCAAAGUCUGGC
|
|
2864
AAAGAUGCAAAGUCUGGCA
|
|
2865
AAGAUGCAAAGUCUGGCAC
|
|
2866
AGAUGCAAAGUCUGGCACC
|
|
2867
GAUGCAAAGUCUGGCACCA
|
|
2868
AUGCAAAGUCUGGCACCAA
|
|
2869
UGCAAAGUCUGGCACCAAG
|
|
2870
GCAAAGUCUGGCACCAAGA
|
|
2871
CAAAGUCUGGCACCAAGAA
|
|
2872
AAAGUCUGGCACCAAGAAG
|
|
2873
AAGUCUGGCACCAAGAAGA
|
|
2874
AGUCUGGCACCAAGAAGAG
|
|
2875
GUCUGGCACCAAGAAGAGG
|
|
2876
UCUGGCACCAAGAAGAGGA
|
|
2877
CUGGCACCAAGAAGAGGAA
|
|
2878
UGGCACCAAGAAGAGGAAG
|
|
2879
GGCACCAAGAAGAGGAAGA
|
|
2880
GCACCAAGAAGAGGAAGAG
|
|
2881
CACCAAGAAGAGGAAGAGC
|
|
2882
ACCAAGAAGAGGAAGAGCA
|
|
2883
CCAAGAAGAGGAAGAGCAA
|
|
2884
CAAGAAGAGGAAGAGCAAA
|
|
2885
AAGAAGAGGAAGAGCAAAC
|
|
2886
AGAAGAGGAAGAGCAAACA
|
|
2887
GAAGAGGAAGAGCAAACAG
|
|
2888
AAGAGGAAGAGCAAACAGA
|
|
2889
AGAGGAAGAGCAAACAGAG
|
|
2890
GAGGAAGAGCAAACAGAGG
|
|
2891
AGGAAGAGCAAACAGAGGG
|
|
2892
GGAAGAGCAAACAGAGGGG
|
|
2893
GAAGAGCAAACAGAGGGGG
|
|
2894
AAGAGCAAACAGAGGGGGG
|
|
2895
AGAGCAAACAGAGGGGGGC
|
|
2896
GAGCAAACAGAGGGGGGCU
|
|
2897
AGCAAACAGAGGGGGGCUG
|
|
2898
GCAAACAGAGGGGGGCUGU
|
|
2899
CAAACAGAGGGGGGCUGUG
|
|
2900
AAACAGAGGGGGGCUGUGG
|
|
2901
AACAGAGGGGGGCUGUGGC
|
|
2902
ACAGAGGGGGGCUGUGGCC
|
|
2903
CAGAGGGGGGCUGUGGCCC
|
|
2904
AGAGGGGGGCUGUGGCCCC
|
|
2905
GAGGGGGGCUGUGGCCCCC
|
|
2906
AGGGGGGCUGUGGCCCCCA
|
|
2907
GGGGGGCUGUGGCCCCCAC
|
|
2908
GGGGGCUGUGGCCCCCACC
|
|
2909
GGGGCUGUGGCCCCCACCA
|
|
2910
GGGCUGUGGCCCCCACCAC
|
|
2911
GGCUGUGGCCCCCACCACA
|
|
2912
GCUGUGGCCCCCACCACAU
|
|
2913
CUGUGGCCCCCACCACAUC
|
|
2914
UGUGGCCCCCACCACAUCC
|
|
2915
GUGGCCCCCACCACAUCCG
|
|
2916
UGGCCCCCACCACAUCCGC
|
|
2917
GGCCCCCACCACAUCCGCC
|
|
2918
GCCCCCACCACAUCCGCCA
|
|
2919
CCCCCACCACAUCCGCCAG
|
|
2920
CCCCACCACAUCCGCCAGC
|
|
2921
CCCACCACAUCCGCCAGCC
|
|
2922
CCACCACAUCCGCCAGCCC
|
|
2923
CACCACAUCCGCCAGCCCU
|
|
2924
ACCACAUCCGCCAGCCCUG
|
|
2925
CCACAUCCGCCAGCCCUGC
|
|
2926
CACAUCCGCCAGCCCUGCC
|
|
2927
ACAUCCGCCAGCCCUGCCC
|
|
2928
CAUCCGCCAGCCCUGCCCA
|
|
2929
AUCCGCCAGCCCUGCCCAA
|
|
2930
UCCGCCAGCCCUGCCCAAU
|
|
2931
CCGCCAGCCCUGCCCAAUA
|
|
2932
CGCCAGCCCUGCCCAAUAG
|
|
2933
GCCAGCCCUGCCCAAUAGC
|
|
2934
CCAGCCCUGCCCAAUAGCU
|
|
2935
CAGCCCUGCCCAAUAGCUG
|
|
2936
AGCCCUGCCCAAUAGCUGC
|
|
2937
GCCCUGCCCAAUAGCUGCC
|
|
2938
CCCUGCCCAAUAGCUGCCC
|
|
2939
CCUGCCCAAUAGCUGCCCC
|
|
2940
CUGCCCAAUAGCUGCCCCU
|
|
2941
UGCCCAAUAGCUGCCCCUU
|
|
2942
GCCCAAUAGCUGCCCCUUU
|
|
2943
CCCAAUAGCUGCCCCUUUA
|
|
2944
CCAAUAGCUGCCCCUUUAC
|
|
2945
CAAUAGCUGCCCCUUUACG
|
|
2946
AAUAGCUGCCCCUUUACGU
|
|
2947
AUAGCUGCCCCUUUACGUC
|
|
2948
UAGCUGCCCCUUUACGUCA
|
|
2949
AGCUGCCCCUUUACGUCAC
|
|
2950
GCUGCCCCUUUACGUCACC
|
|
2951
CUGCCCCUUUACGUCACCU
|
|
2952
UGCCCCUUUACGUCACCUG
|
|
2953
GCCCCUUUACGUCACCUGA
|
|
2954
CCCCUUUACGUCACCUGAC
|
|
2955
CCCUUUACGUCACCUGACG
|
|
2956
CCUUUACGUCACCUGACGG
|
|
2957
CUUUACGUCACCUGACGGC
|
|
2958
UUUACGUCACCUGACGGCA
|
|
2959
UUACGUCACCUGACGGCAA
|
|
2960
UACGUCACCUGACGGCAAG
|
|
2961
ACGUCACCUGACGGCAAGA
|
|
2962
CGUCACCUGACGGCAAGAC
|
|
2963
GUCACCUGACGGCAAGACU
|
|
2964
UCACCUGACGGCAAGACUU
|
|
2965
CACCUGACGGCAAGACUUC
|
|
2966
ACCUGACGGCAAGACUUCA
|
|
2967
CCUGACGGCAAGACUUCAU
|
|
2968
CUGACGGCAAGACUUCAUU
|
|
2969
UGACGGCAAGACUUCAUUG
|
|
2970
GACGGCAAGACUUCAUUGC
|
|
2971
ACGGCAAGACUUCAUUGCU
|
|
2972
CGGCAAGACUUCAUUGCUG
|
|
2973
GGCAAGACUUCAUUGCUGC
|
|
2974
GCAAGACUUCAUUGCUGCU
|
|
2975
CAAGACUUCAUUGCUGCUA
|
|
2976
AAGACUUCAUUGCUGCUAU
|
|
2977
AGACUUCAUUGCUGCUAUG
|
|
2978
GACUUCAUUGCUGCUAUGU
|
|
2979
ACUUCAUUGCUGCUAUGUA
|
|
2980
CUUCAUUGCUGCUAUGUAU
|
|
2981
UUCAUUGCUGCUAUGUAUA
|
|
2982
UCAUUGCUGCUAUGUAUAU
|
|
2983
CAUUGCUGCUAUGUAUAUG
|
|
2984
AUUGCUGCUAUGUAUAUGA
|
|
2985
UUGCUGCUAUGUAUAUGAA
|
|
2986
UGCUGCUAUGUAUAUGAAA
|
|
2987
GCUGCUAUGUAUAUGAAAG
|
|
2988
CUGCUAUGUAUAUGAAAGC
|
|
2989
UGCUAUGUAUAUGAAAGCU
|
|
2990
GCUAUGUAUAUGAAAGCUU
|
|
2991
CUAUGUAUAUGAAAGCUUU
|
|
2992
UAUGUAUAUGAAAGCUUUA
|
|
2993
AUGUAUAUGAAAGCUUUAU
|
|
2994
UGUAUAUGAAAGCUUUAUU
|
|
2995
GUAUAUGAAAGCUUUAUUG
|
|
2996
UAUAUGAAAGCUUUAUUGA
|
|
2997
AUAUGAAAGCUUUAUUGAA
|
|
2998
UAUGAAAGCUUUAUUGAAC
|
|
2999
AUGAAAGCUUUAUUGAACC
|
|
3000
UGAAAGCUUUAUUGAACCA
|
|
3001
GAAAGCUUUAUUGAACCAG
|
|
3002
AAAGCUUUAUUGAACCAGA
|
|
3003
AAGCUUUAUUGAACCAGAG
|
|
3004
AGCUUUAUUGAACCAGAGC
|
|
3005
GCUUUAUUGAACCAGAGCA
|
|
3006
CUUUAUUGAACCAGAGCAC
|
|
3007
UUUAUUGAACCAGAGCACU
|
|
3008
UUAUUGAACCAGAGCACUG
|
|
3009
UAUUGAACCAGAGCACUGC
|
|
3010
AUUGAACCAGAGCACUGCU
|
|
3011
UUGAACCAGAGCACUGCUA
|
|
3012
UGAACCAGAGCACUGCUAC
|
|
3013
GAACCAGAGCACUGCUACA
|
|
3014
AACCAGAGCACUGCUACAC
|
|
3015
ACCAGAGCACUGCUACACA
|
|
3016
CCAGAGCACUGCUACACAA
|
|
3017
CAGAGCACUGCUACACAAC
|
|
3018
AGAGCACUGCUACACAACA
|
|
3019
GAGCACUGCUACACAACAU
|
|
3020
AGCACUGCUACACAACAUU
|
|
3021
GCACUGCUACACAACAUUA
|
|
3022
CACUGCUACACAACAUUAC
|
|
3023
ACUGCUACACAACAUUACA
|
|
3024
CUGCUACACAACAUUACAC
|
|
3025
UGCUACACAACAUUACACA
|
|
3026
GCUACACAACAUUACACAU
|
|
3027
CUACACAACAUUACACAUG
|
|
3028
UACACAACAUUACACAUGU
|
|
3029
ACACAACAUUACACAUGUC
|
|
3030
CACAACAUUACACAUGUCA
|
|
3031
ACAACAUUACACAUGUCAG
|
|
3032
CAACAUUACACAUGUCAGA
|
|
3033
AACAUUACACAUGUCAGAA
|
|
3034
ACAUUACACAUGUCAGAAA
|
|
3035
CAUUACACAUGUCAGAAAG
|
|
3036
AUUACACAUGUCAGAAAGA
|
|
3037
UUACACAUGUCAGAAAGAC
|
|
3038
UACACAUGUCAGAAAGACA
|
|
3039
ACACAUGUCAGAAAGACAG
|
|
3040
CACAUGUCAGAAAGACAGA
|
|
3041
ACAUGUCAGAAAGACAGAG
|
|
3042
CAUGUCAGAAAGACAGAGC
|
|
3043
AUGUCAGAAAGACAGAGCU
|
|
3044
UGUCAGAAAGACAGAGCUA
|
|
3045
GUCAGAAAGACAGAGCUAU
|
|
3046
UCAGAAAGACAGAGCUAUA
|
|
3047
CAGAAAGACAGAGCUAUAC
|
|
3048
AGAAAGACAGAGCUAUACU
|
|
3049
GAAAGACAGAGCUAUACUC
|
|
3050
AAAGACAGAGCUAUACUCC
|
|
3051
AAGACAGAGCUAUACUCCU
|
|
3052
AGACAGAGCUAUACUCCUA
|
|
3053
GACAGAGCUAUACUCCUAG
|
|
3054
ACAGAGCUAUACUCCUAGA
|
|
3055
CAGAGCUAUACUCCUAGAC
|
|
3056
AGAGCUAUACUCCUAGACU
|
|
3057
GAGCUAUACUCCUAGACUC
|
|
3058
AGCUAUACUCCUAGACUCG
|
|
3059
GCUAUACUCCUAGACUCGA
|
|
3060
CUAUACUCCUAGACUCGAC
|
|
3061
UAUACUCCUAGACUCGACA
|
|
3062
AUACUCCUAGACUCGACAG
|
|
3063
UACUCCUAGACUCGACAGA
|
|
3064
ACUCCUAGACUCGACAGAA
|
|
3065
CUCCUAGACUCGACAGAAG
|
|
3066
UCCUAGACUCGACAGAAGC
|
|
3067
CCUAGACUCGACAGAAGCC
|
|
3068
CUAGACUCGACAGAAGCCA
|
|
3069
UAGACUCGACAGAAGCCAC
|
|
3070
AGACUCGACAGAAGCCACA
|
|
3071
GACUCGACAGAAGCCACAU
|
|
3072
ACUCGACAGAAGCCACAUC
|
|
3073
CUCGACAGAAGCCACAUCC
|
|
3074
UCGACAGAAGCCACAUCCA
|
|
3075
CGACAGAAGCCACAUCCAC
|
|
3076
GACAGAAGCCACAUCCACA
|
|
3077
ACAGAAGCCACAUCCACAA
|
|
3078
CAGAAGCCACAUCCACAAC
|
|
3079
AGAAGCCACAUCCACAACA
|
|
3080
GAAGCCACAUCCACAACAC
|
|
3081
AAGCCACAUCCACAACACU
|
|
3082
AGCCACAUCCACAACACUU
|
|
3083
GCCACAUCCACAACACUUA
|
|
3084
CCACAUCCACAACACUUAA
|
|
3085
CACAUCCACAACACUUAAG
|
|
3086
ACAUCCACAACACUUAAGG
|
|
3087
CAUCCACAACACUUAAGGA
|
|
3088
AUCCACAACACUUAAGGAG
|
|
3089
UCCACAACACUUAAGGAGG
|
|
3090
CCACAACACUUAAGGAGGC
|
|
3091
CACAACACUUAAGGAGGCG
|
|
3092
ACAACACUUAAGGAGGCGG
|
|
3093
CAACACUUAAGGAGGCGGU
|
|
3094
AACACUUAAGGAGGCGGUA
|
|
3095
ACACUUAAGGAGGCGGUAC
|
|
3096
CACUUAAGGAGGCGGUACC
|
|
3097
ACUUAAGGAGGCGGUACCC
|
|
3098
CUUAAGGAGGCGGUACCCC
|
|
3099
UUAAGGAGGCGGUACCCCC
|
|
3100
UAAGGAGGCGGUACCCCCG
|
|
3101
AAGGAGGCGGUACCCCCGG
|
|
3102
AGGAGGCGGUACCCCCGGC
|
|
3103
GGAGGCGGUACCCCCGGCA
|
|
3104
GAGGCGGUACCCCCGGCAC
|
|
3105
AGGCGGUACCCCCGGCACC
|
|
3106
GGCGGUACCCCCGGCACCA
|
|
3107
GCGGUACCCCCGGCACCAU
|
|
3108
CGGUACCCCCGGCACCAUG
|
|
3109
GGUACCCCCGGCACCAUGA
|
|
3110
GUACCCCCGGCACCAUGAA
|
|
3111
UACCCCCGGCACCAUGAAU
|
|
3112
ACCCCCGGCACCAUGAAUG
|
|
3113
CCCCCGGCACCAUGAAUGG
|
|
3114
CCCCGGCACCAUGAAUGGC
|
|
3115
CCCGGCACCAUGAAUGGCA
|
|
3116
CCGGCACCAUGAAUGGCAU
|
|
3117
CGGCACCAUGAAUGGCAUC
|
|
3118
GGCACCAUGAAUGGCAUCC
|
|
3119
GCACCAUGAAUGGCAUCCA
|
|
3120
CACCAUGAAUGGCAUCCAU
|
|
3121
ACCAUGAAUGGCAUCCAUU
|
|
3122
CCAUGAAUGGCAUCCAUUG
|
|
3123
CAUGAAUGGCAUCCAUUGG
|
|
3124
AUGAAUGGCAUCCAUUGGG
|
|
3125
UGAAUGGCAUCCAUUGGGG
|
|
3126
GAAUGGCAUCCAUUGGGGC
|
|
3127
AAUGGCAUCCAUUGGGGCA
|
|
3128
AUGGCAUCCAUUGGGGCAG
|
|
3129
UGGCAUCCAUUGGGGCAGU
|
|
3130
GGCAUCCAUUGGGGCAGUG
|
|
3131
GCAUCCAUUGGGGCAGUGG
|
|
3132
CAUCCAUUGGGGCAGUGGG
|
|
3133
AUCCAUUGGGGCAGUGGGA
|
|
3134
UCCAUUGGGGCAGUGGGAC
|
|
3135
CCAUUGGGGCAGUGGGACA
|
|
3136
CAUUGGGGCAGUGGGACAC
|
|
3137
AUUGGGGCAGUGGGACACU
|
|
3138
UUGGGGCAGUGGGACACUG
|
|
3139
UGGGGCAGUGGGACACUGC
|
|
3140
GGGGCAGUGGGACACUGCA
|
|
3141
GGGCAGUGGGACACUGCAG
|
|
3142
GGCAGUGGGACACUGCAGG
|
|
3143
GCAGUGGGACACUGCAGGA
|
|
3144
CAGUGGGACACUGCAGGAC
|
|
3145
AGUGGGACACUGCAGGACC
|
|
3146
GUGGGACACUGCAGGACCA
|
|
3147
UGGGACACUGCAGGACCAG
|
|
3148
GGGACACUGCAGGACCAGA
|
|
3149
GGACACUGCAGGACCAGAG
|
|
3150
GACACUGCAGGACCAGAGG
|
|
3151
ACACUGCAGGACCAGAGGU
|
|
3152
CACUGCAGGACCAGAGGUG
|
|
3153
ACUGCAGGACCAGAGGUGA
|
|
3154
CUGCAGGACCAGAGGUGAG
|
|
3155
UGCAGGACCAGAGGUGAGG
|
|
3156
GCAGGACCAGAGGUGAGGA
|
|
3157
CAGGACCAGAGGUGAGGAU
|
|
3158
AGGACCAGAGGUGAGGAUG
|
|
3159
GGACCAGAGGUGAGGAUGA
|
|
3160
GACCAGAGGUGAGGAUGAA
|
|
3161
ACCAGAGGUGAGGAUGAAC
|
|
3162
CCAGAGGUGAGGAUGAACC
|
|
3163
CAGAGGUGAGGAUGAACCA
|
|
3164
AGAGGUGAGGAUGAACCAA
|
|
3165
GAGGUGAGGAUGAACCAAG
|
|
3166
AGGUGAGGAUGAACCAAGG
|
|
3167
GGUGAGGAUGAACCAAGGA
|
|
3168
GUGAGGAUGAACCAAGGAU
|
|
3169
UGAGGAUGAACCAAGGAUG
|
|
3170
GAGGAUGAACCAAGGAUGG
|
|
3171
AGGAUGAACCAAGGAUGGG
|
|
3172
GGAUGAACCAAGGAUGGGG
|
|
3173
GAUGAACCAAGGAUGGGGG
|
|
3174
AUGAACCAAGGAUGGGGGC
|
|
3175
UGAACCAAGGAUGGGGGCA
|
|
3176
GAACCAAGGAUGGGGGCAU
|
|
3177
AACCAAGGAUGGGGGCAUG
|
|
3178
ACCAAGGAUGGGGGCAUGG
|
|
3179
CCAAGGAUGGGGGCAUGGG
|
|
3180
CAAGGAUGGGGGCAUGGGG
|
|
3181
AAGGAUGGGGGCAUGGGGC
|
|
3182
AGGAUGGGGGCAUGGGGCC
|
|
3183
GGAUGGGGGCAUGGGGCCU
|
|
3184
GAUGGGGGCAUGGGGCCUU
|
|
3185
AUGGGGGCAUGGGGCCUUG
|
|
3186
UGGGGGCAUGGGGCCUUGG
|
|
3187
GGGGGCAUGGGGCCUUGGG
|
|
3188
GGGGCAUGGGGCCUUGGGA
|
|
3189
GGGCAUGGGGCCUUGGGAC
|
|
3190
GGCAUGGGGCCUUGGGACA
|
|
3191
GCAUGGGGCCUUGGGACAC
|
|
3192
CAUGGGGCCUUGGGACACU
|
|
3193
AUGGGGCCUUGGGACACUU
|
|
3194
UGGGGCCUUGGGACACUUG
|
|
3195
GGGGCCUUGGGACACUUGC
|
|
3196
GGGCCUUGGGACACUUGCC
|
|
3197
GGCCUUGGGACACUUGCCU
|
|
3198
GCCUUGGGACACUUGCCUU
|
|
3199
CCUUGGGACACUUGCCUUG
|
|
3200
CUUGGGACACUUGCCUUGU
|
|
3201
UUGGGACACUUGCCUUGUG
|
|
3202
UGGGACACUUGCCUUGUGC
|
|
3203
GGGACACUUGCCUUGUGCC
|
|
3204
GGACACUUGCCUUGUGCCC
|
|
3205
GACACUUGCCUUGUGCCCA
|
|
3206
ACACUUGCCUUGUGCCCAG
|
|
3207
CACUUGCCUUGUGCCCAGC
|
|
3208
ACUUGCCUUGUGCCCAGCC
|
|
3209
CUUGCCUUGUGCCCAGCCA
|
|
3210
UUGCCUUGUGCCCAGCCAG
|
|
3211
UGCCUUGUGCCCAGCCAGC
|
|
3212
GCCUUGUGCCCAGCCAGCC
|
|
3213
CCUUGUGCCCAGCCAGCCA
|
|
3214
CUUGUGCCCAGCCAGCCAC
|
|
3215
UUGUGCCCAGCCAGCCACG
|
|
3216
UGUGCCCAGCCAGCCACGU
|
|
3217
GUGCCCAGCCAGCCACGUG
|
|
3218
UGCCCAGCCAGCCACGUGG
|
|
3219
GCCCAGCCAGCCACGUGGA
|
|
3220
CCCAGCCAGCCACGUGGAC
|
|
3221
CCAGCCAGCCACGUGGACU
|
|
3222
CAGCCAGCCACGUGGACUG
|
|
3223
AGCCAGCCACGUGGACUGG
|
|
3224
GCCAGCCACGUGGACUGGA
|
|
3225
CCAGCCACGUGGACUGGAU
|
|
3226
CAGCCACGUGGACUGGAUU
|
|
3227
AGCCACGUGGACUGGAUUU
|
|
3228
GCCACGUGGACUGGAUUUC
|
|
3229
CCACGUGGACUGGAUUUCU
|
|
3230
CACGUGGACUGGAUUUCUG
|
|
3231
ACGUGGACUGGAUUUCUGC
|
|
3232
CGUGGACUGGAUUUCUGCU
|
|
3233
GUGGACUGGAUUUCUGCUC
|
|
3234
UGGACUGGAUUUCUGCUCU
|
|
3235
GGACUGGAUUUCUGCUCUU
|
|
3236
GACUGGAUUUCUGCUCUUC
|
|
3237
ACUGGAUUUCUGCUCUUCC
|
|
3238
CUGGAUUUCUGCUCUUCCA
|
|
3239
UGGAUUUCUGCUCUUCCAG
|
|
3240
GGAUUUCUGCUCUUCCAGA
|
|
3241
GAUUUCUGCUCUUCCAGAC
|
|
3242
AUUUCUGCUCUUCCAGACC
|
|
3243
UUUCUGCUCUUCCAGACCG
|
|
3244
UUCUGCUCUUCCAGACCGG
|
|
3245
UCUGCUCUUCCAGACCGGG
|
|
3246
CUGCUCUUCCAGACCGGGG
|
|
3247
UGCUCUUCCAGACCGGGGA
|
|
3248
GCUCUUCCAGACCGGGGAA
|
|
3249
CUCUUCCAGACCGGGGAAC
|
|
3250
UCUUCCAGACCGGGGAACU
|
|
3251
CUUCCAGACCGGGGAACUG
|
|
3252
UUCCAGACCGGGGAACUGG
|
|
3253
UCCAGACCGGGGAACUGGA
|
|
3254
CCAGACCGGGGAACUGGAC
|
|
3255
CAGACCGGGGAACUGGACU
|
|
3256
AGACCGGGGAACUGGACUC
|
|
3257
GACCGGGGAACUGGACUCA
|
|
3258
ACCGGGGAACUGGACUCAC
|
|
3259
CCGGGGAACUGGACUCACA
|
|
3260
CGGGGAACUGGACUCACAU
|
|
3261
GGGGAACUGGACUCACAUA
|
|
3262
GGGAACUGGACUCACAUAA
|
|
3263
GGAACUGGACUCACAUAAA
|
|
3264
GAACUGGACUCACAUAAAG
|
|
3265
AACUGGACUCACAUAAAGG
|
|
3266
ACUGGACUCACAUAAAGGC
|
|
3267
CUGGACUCACAUAAAGGCA
|
|
3268
UGGACUCACAUAAAGGCAA
|
|
3269
GGACUCACAUAAAGGCAAU
|
|
3270
GACUCACAUAAAGGCAAUG
|
|
3271
ACUCACAUAAAGGCAAUGU
|
|
3272
CUCACAUAAAGGCAAUGUC
|
|
3273
UCACAUAAAGGCAAUGUCC
|
|
3274
CACAUAAAGGCAAUGUCCU
|
|
3275
ACAUAAAGGCAAUGUCCUC
|
|
3276
CAUAAAGGCAAUGUCCUCU
|
|
3277
AUAAAGGCAAUGUCCUCUU
|
|
3278
UAAAGGCAAUGUCCUCUUU
|
|
3279
AAAGGCAAUGUCCUCUUUC
|
|
3280
AAGGCAAUGUCCUCUUUCU
|
|
3281
AGGCAAUGUCCUCUUUCUC
|
|
3282
GGCAAUGUCCUCUUUCUCU
|
|
3283
GCAAUGUCCUCUUUCUCUU
|
|
3284
CAAUGUCCUCUUUCUCUUC
|
|
3285
AAUGUCCUCUUUCUCUUCC
|
|
3286
AUGUCCUCUUUCUCUUCCC
|
|
3287
UGUCCUCUUUCUCUUCCCC
|
|
3288
GUCCUCUUUCUCUUCCCCC
|
|
3289
UCCUCUUUCUCUUCCCCCC
|
|
3290
CCUCUUUCUCUUCCCCCCA
|
|
3291
CUCUUUCUCUUCCCCCCAA
|
|
3292
UCUUUCUCUUCCCCCCAAC
|
|
3293
CUUUCUCUUCCCCCCAACC
|
|
3294
UUUCUCUUCCCCCCAACCC
|
|
3295
UUCUCUUCCCCCCAACCCU
|
|
3296
UCUCUUCCCCCCAACCCUU
|
|
3297
CUCUUCCCCCCAACCCUUU
|
|
3298
UCUUCCCCCCAACCCUUUA
|
|
3299
CUUCCCCCCAACCCUUUAU
|
|
3300
UUCCCCCCAACCCUUUAUU
|
|
3301
UCCCCCCAACCCUUUAUUU
|
|
3302
CCCCCCAACCCUUUAUUUU
|
|
3303
CCCCCAACCCUUUAUUUUG
|
|
3304
CCCCAACCCUUUAUUUUGU
|
|
3305
CCCAACCCUUUAUUUUGUG
|
|
3306
CCAACCCUUUAUUUUGUGU
|
|
3307
CAACCCUUUAUUUUGUGUU
|
|
3308
AACCCUUUAUUUUGUGUUU
|
|
3309
ACCCUUUAUUUUGUGUUUU
|
|
3310
CCCUUUAUUUUGUGUUUUA
|
|
3311
CCUUUAUUUUGUGUUUUAA
|
|
3312
CUUUAUUUUGUGUUUUAAG
|
|
3313
UUUAUUUUGUGUUUUAAGC
|
|
3314
UUAUUUUGUGUUUUAAGCU
|
|
3315
UAUUUUGUGUUUUAAGCUG
|
|
3316
AUUUUGUGUUUUAAGCUGU
|
|
3317
UUUUGUGUUUUAAGCUGUA
|
|
3318
UUUGUGUUUUAAGCUGUAU
|
|
3319
UUGUGUUUUAAGCUGUAUG
|
|
3320
UGUGUUUUAAGCUGUAUGA
|
|
3321
GUGUUUUAAGCUGUAUGAC
|
|
3322
UGUUUUAAGCUGUAUGACU
|
|
3323
GUUUUAAGCUGUAUGACUU
|
|
3324
UUUUAAGCUGUAUGACUUU
|
|
3325
UUUAAGCUGUAUGACUUUA
|
|
3326
UUAAGCUGUAUGACUUUAU
|
|
3327
UAAGCUGUAUGACUUUAUC
|
|
3328
AAGCUGUAUGACUUUAUCA
|
|
3329
AGCUGUAUGACUUUAUCAC
|
|
3330
GCUGUAUGACUUUAUCACU
|
|
3331
CUGUAUGACUUUAUCACUG
|
|
3332
UGUAUGACUUUAUCACUGA
|
|
3333
GUAUGACUUUAUCACUGAG
|
|
3334
UAUGACUUUAUCACUGAGA
|
|
3335
AUGACUUUAUCACUGAGAA
|
|
3336
UGACUUUAUCACUGAGAAU
|
|
3337
GACUUUAUCACUGAGAAUA
|
|
3338
ACUUUAUCACUGAGAAUAA
|
|
3339
CUUUAUCACUGAGAAUAAU
|
|
3340
UUUAUCACUGAGAAUAAUA
|
|
3341
UUAUCACUGAGAAUAAUAC
|
|
3342
UAUCACUGAGAAUAAUACA
|
|
3343
AUCACUGAGAAUAAUACAU
|
|
3344
UCACUGAGAAUAAUACAUG
|
|
3345
CACUGAGAAUAAUACAUGU
|
|
3346
ACUGAGAAUAAUACAUGUU
|
|
3347
CUGAGAAUAAUACAUGUUA
|
|
3348
UGAGAAUAAUACAUGUUAA
|
|
3349
GAGAAUAAUACAUGUUAAA
|
|
3350
AGAAUAAUACAUGUUAAAC
|
|
3351
GAAUAAUACAUGUUAAACG
|
|
3352
AAUAAUACAUGUUAAACGU
|
|
3353
AUAAUACAUGUUAAACGUU
|
|
3354
UAAUACAUGUUAAACGUUU
|
|
3355
AAUACAUGUUAAACGUUUG
|
|
3356
AUACAUGUUAAACGUUUGU
|
|
3357
UACAUGUUAAACGUUUGUG
|
|
3358
ACAUGUUAAACGUUUGUGG
|
|
3359
CAUGUUAAACGUUUGUGGU
|
|
3360
AUGUUAAACGUUUGUGGUA
|
|
3361
UGUUAAACGUUUGUGGUAA
|
|
3362
GUUAAACGUUUGUGGUAAG
|
|
3363
UUAAACGUUUGUGGUAAGA
|
|
3364
UAAACGUUUGUGGUAAGAG
|
|
3365
AAACGUUUGUGGUAAGAGG
|
|
3366
AACGUUUGUGGUAAGAGGU
|
|
3367
ACGUUUGUGGUAAGAGGUC
|
|
3368
CGUUUGUGGUAAGAGGUCA
|
|
3369
GUUUGUGGUAAGAGGUCAG
|
|
3370
UUUGUGGUAAGAGGUCAGU
|
|
3371
UUGUGGUAAGAGGUCAGUG
|
|
3372
UGUGGUAAGAGGUCAGUGG
|
|
3373
GUGGUAAGAGGUCAGUGGU
|
|
3374
UGGUAAGAGGUCAGUGGUA
|
|
3375
GGUAAGAGGUCAGUGGUAU
|
|
3376
GUAAGAGGUCAGUGGUAUC
|
|
3377
UAAGAGGUCAGUGGUAUCU
|
|
3378
AAGAGGUCAGUGGUAUCUG
|
|
3379
AGAGGUCAGUGGUAUCUGC
|
|
3380
GAGGUCAGUGGUAUCUGCC
|
|
3381
AGGUCAGUGGUAUCUGCCC
|
|
3382
GGUCAGUGGUAUCUGCCCU
|
|
3383
GUCAGUGGUAUCUGCCCUG
|
|
3384
UCAGUGGUAUCUGCCCUGA
|
|
3385
CAGUGGUAUCUGCCCUGAA
|
|
3386
AGUGGUAUCUGCCCUGAAU
|
|
3387
GUGGUAUCUGCCCUGAAUC
|
|
3388
UGGUAUCUGCCCUGAAUCU
|
|
3389
GGUAUCUGCCCUGAAUCUG
|
|
3390
GUAUCUGCCCUGAAUCUGC
|
|
3391
UAUCUGCCCUGAAUCUGCU
|
|
3392
AUCUGCCCUGAAUCUGCUU
|
|
3393
UCUGCCCUGAAUCUGCUUC
|
|
3394
CUGCCCUGAAUCUGCUUCA
|
|
3395
UGCCCUGAAUCUGCUUCAA
|
|
3396
GCCCUGAAUCUGCUUCAAA
|
|
3397
CCCUGAAUCUGCUUCAAAG
|
|
3398
CCUGAAUCUGCUUCAAAGA
|
|
3399
CUGAAUCUGCUUCAAAGAG
|
|
3400
UGAAUCUGCUUCAAAGAGU
|
|
3401
GAAUCUGCUUCAAAGAGUU
|
|
3402
AAUCUGCUUCAAAGAGUUA
|
|
3403
AUCUGCUUCAAAGAGUUAU
|
|
3404
UCUGCUUCAAAGAGUUAUU
|
|
3405
CUGCUUCAAAGAGUUAUUU
|
|
3406
UGCUUCAAAGAGUUAUUUC
|
|
3407
GCUUCAAAGAGUUAUUUCA
|
|
3408
CUUCAAAGAGUUAUUUCAA
|
|
3409
UUCAAAGAGUUAUUUCAAA
|
|
3410
UCAAAGAGUUAUUUCAAAU
|
|
3411
CAAAGAGUUAUUUCAAAUU
|
|
3412
AAAGAGUUAUUUCAAAUUA
|
|
3413
AAGAGUUAUUUCAAAUUAA
|
|
3414
AGAGUUAUUUCAAAUUAAA
|
|
3415
GAGUUAUUUCAAAUUAAAA
|
|
3416
AGUUAUUUCAAAUUAAAAG
|
|
3417
GUUAUUUCAAAUUAAAAGC
|
|
3418
UUAUUUCAAAUUAAAAGCA
|
|
3419
UAUUUCAAAUUAAAAGCAA
|
|
3420
AUUUCAAAUUAAAAGCAAA
|
|
3421
UUUCAAAUUAAAAGCAAAA
|
|
3422
UUCAAAUUAAAAGCAAAAC
|
|
3423
UCAAAUUAAAAGCAAAACA
|
|
3424
CAAAUUAAAAGCAAAACAA
|
|
3425
AAAUUAAAAGCAAAACAAA
|
|
3426
AAUUAAAAGCAAAACAAAA
|
|
3427
AUUAAAAGCAAAACAAAAC
|
|
3428
UUAAAAGCAAAACAAAACA
|
|
3429
UAAAAGCAAAACAAAACAA
|
|
3430
AAAAGCAAAACAAAACAAA
|
|
The double-stranded regions should be at least 19 contiguous nucleotides, for example about 19 to 23 nucleotides, or may be longer, for example 30 or 50 nucleotides, or 100 nucleotides or more. The full-length sequence corresponding to the entire gene transcript may be used. Preferably, they are about 19 to about 100 nucleotides in length, more preferably about 19 to about 50 nucleotides in length, and even more preferably about 19 to about 23 nucleotides in length.
The degree of identity of a double-stranded region of a nucleic acid molecule to the targeted transcript should be at least 90% and more preferably 95-100%. The % identity of a nucleic acid molecule is determined by GAP (Needleman and Wunsch, 1970) analysis (GCG program) with a gap creation penalty=5, and a gap extension penalty=0.3. Preferably, the two sequences are aligned over their entire length.
The nucleic acid molecule may of course comprise sequences unrelated to the target which may function to stabilize the molecule.
The term “short interfering RNA” or “siRNA” as used herein refers to a nucleic acid molecule which comprises ribonucleotides capable of inhibiting or down regulating gene expression, for example by mediating RNAi in a sequence-specific manner, wherein the double stranded portion is less than 50 nucleotides in length, preferably about 19 to about 23 nucleotides in length. For example, the siRNA can be a nucleic acid molecule comprising self-complementary sense and antisense regions, wherein the antisense region comprises a nucleotide sequence that is complementary to nucleotide sequence in a target nucleic acid molecule or a portion thereof and the sense region having nucleotide sequence corresponding to the target nucleic acid sequence or a portion thereof. The siRNA can be assembled from two separate oligonucleotides, where one strand is the sense strand and the other is the antisense strand, wherein the antisense and sense strands are self-complementary.
As used herein, the term siRNA is equivalent to other terms used to describe nucleic acid molecules that are capable of mediating sequence specific RNAi, for example micro-RNA (miRNA), short hairpin RNA (shRNA), short interfering oligonucleotide, short interfering nucleic acid (siNA), short interfering modified oligonucleotide, chemically-modified siRNA, and others. In addition, as used herein, the term RNAi is equivalent to other terms used to describe sequence specific RNA interference, such as post transcriptional gene silencing, translational inhibition, or epigenetics. For example, siRNA molecules of the invention can be used to epigenetically silence genes at both the post-transcriptional level or the pre-transcriptional level. In a non-limiting example, epigenetic regulation of gene expression by siRNA molecules of the invention can result from siRNA mediated modification of chromatin structure to alter gene expression.
Preferred siRNA molecules comprise a nucleotide sequence that is identical to about 19 to 23 contiguous nucleotides of the target mRNA. In an embodiment, the target mRNA sequence commences with the dinucleotide AA, comprises a GC-content of about 30-70% (preferably, 30-60%, more preferably 40-60% and more preferably about 45%-55%), and does not have a high percentage identity to any nucleotide sequence other than the target in the genome of the avian (preferably chickens) in which it is to be introduced, e.g., as determined by standard BLAST search.
By “shRNA” or “short-hairpin RNA” is meant an siRNA molecule where less than about 50 nucleotides, preferably about 19 to about 23 nucleotides, is base paired with a complementary sequence located on the same RNA molecule, and where said sequence and complementary sequence are separated by an unpaired region of at least about 4 to 15 nucleotides which forms a single-stranded loop above the stem structure created by the two regions of base complementarity. Examples of sequences of a single-stranded loops are 5′ UUCAAGAGA 3′ and 5′ UUUGUGUAG 3′.
Included shRNAs are dual or bi-finger and multi-finger hairpin dsRNAs, in which the RNA molecule comprises two or more of such stem-loop structures separated by single-stranded spacer regions.
siRNAs can be generated in vitro by using a recombinant enzyme, such as T7 RNA polymerase, and DNA oligonucleotide templates, or can be prepared in vivo, for example, in cultured cells. In a preferred embodiment, the nucleic acid molecule is produced synthetically.
Strategies have been described for producing a hairpin siRNA from vectors containing, for example, a RNA polymerase III promoter. Various vectors have been constructed for generating hairpin siRNAs in host cells using either an H1-RNA or an snU6 RNA promoter (see SEQ ID NO's 7 to 9). A RNA molecule as described above (e.g., a first portion, a linking sequence, and a second portion) can be operably linked to such a promoter. When transcribed by RNA polymerase III, the first and second portions form a duplexed stem of a hairpin and the linking sequence forms a loop. The pSuper vector (OligoEngines Ltd., Seattle, Wash.) can also be used to generate siRNA.
Modifications or analogs of nucleotides can be introduced to improve the properties of the nucleic acid molecules of the invention. Improved properties include increased nuclease resistance and/or increased ability to permeate cell membranes. Accordingly, the terms “nucleic acid molecule” and “double-stranded RNA molecule” includes synthetically modified bases such as, but not limited to, inosine, xanthine, hypoxanthine, 2-aminoadenine, 6-methyl-, 2-propyl- and other alkyl-adenines, 5-halo uracil, 5-halo cytosine, 6-aza cytosine and 6-aza thymine, pseudo uracil, 4-thiuracil, 8-halo adenine, 8-aminoadenine, 8-thiol adenine, 8-thiolalkyl adenines, 8-hydroxyl adenine and other 8-substituted adenines, 8-halo guanines, 8-amino guanine, 8-thiol guanine, 8-thioalkyl guanines, 8-hydroxyl guanine and other substituted guanines, other aza and deaza adenines, other aza and deaza guanines, 5-trifluoromethyl uracil and 5-trifluoro cytosine.
Vectors and Host Cells
The present invention also provides a vector encoding a nucleic acid molecule comprising a double-stranded region, or single strand thereof, of the present invention. Preferably, the vector is an expression vector capable of expressing the open reading frame(s) encoding a dsRNA in a host cell and/or cell free system. The host cell can be any cell type such as, not limited to, bacterial, fungal, plant or animal cells, preferably an avian cell.
Typically, a vector of the invention comprises a promoter operably linked to an open reading frame encoding a nucleic acid molecule of the invention, or a strand thereof.
As used herein, the term “promoter” refers to a nucleic acid sequence which is able to direct transcription of an operably linked nucleic acid molecule and includes, for example, RNA polymerase II and RNA polymerase III promoters. Also included in this definition are those transcriptional regulatory elements (e.g., enhancers) that are sufficient to render promoter-dependent gene expression controllable in a cell type-specific, tissue-specific, or temporal-specific manner, or that are inducible by external agents or signals.
“Operably linked” as used herein refers to a functional relationship between two or more nucleic acid (e.g., DNA) segments. Typically, it refers to the functional relationship of a transcriptional regulatory element to a transcribed sequence. For example, a promoter is operably linked to a coding sequence, such as an open reading frame encoding a double-stranded RNA molecule defined herein, if it stimulates or modulates the transcription of the coding sequence in an appropriate cell. Generally, promoter transcriptional regulatory elements that are operably linked to a transcribed sequence are physically contiguous to the transcribed sequence, i.e., they are cis-acting. However, some transcriptional regulatory elements, such as enhancers, need not be physically contiguous or located in close proximity to the coding sequences whose transcription they enhance.
By “RNA polymerase III promoter” or “RNA pol III promoter” or “polymerase III promoter” or “pol III promoter” is meant any invertebrate, vertebrate, or mammalian promoter, e.g., chicken, human, murine, porcine, bovine, primate, simian, etc. that, in its native context in a cell, associates or interacts with RNA polymerase III to transcribe its operably linked gene, or any variant thereof, natural or engineered, that will interact in a selected host cell with an RNA polymerase III to transcribe an operably linked nucleic acid sequence. By U6 promoter (e.g., chicken U6, human U6, murine U6), H1 promoter, or 7SK promoter is meant any invertebrate, vertebrate, or mammalian promoter or polymorphic variant or mutant found in nature to interact with RNA polymerase III to transcribe its cognate RNA product, i.e., U6 RNA, H1 RNA, or 7SK RNA, respectively. Examples of suitable promoters include cU6-1 (SEQ ID NO:7), cU6-3 (SEQ ID NO:8), cU6-4 (SEQ ID NO:9) and c7SK (SEQ ID NO:10).
When E. coli is used as a host cell, there is no limitation other than that the vector should have an “ori” to amplify and mass-produce the vector in E. coli (e.g., JM109. DH5α, HB101, or XL1Blue), and a marker gene for selecting the transformed E. coli (e.g., a drug-resistance gene selected by a drug such as ampicillin, tetracycline, kanamycin, or chloramphenicol). For example, M13-series vectors, pUC-series vectors, pBR322, pBluescript, pCR-Script, and such can be used. pGEM-T, pDIRECT, pT7, and so on can also be used for subcloning and excision of the gene encoding the dsRNA as well as the vectors described above.
With regard to expression vectors for use in E. coli, such vectors include JM109, DH5α, HB101, or XL1 Blue, the vector should have a promoter such as lacZ promoter, araB promoter, or T7 promoter that can efficiently promote the expression of the desired gene in E. coli. Other examples of the vectors are “QIAexpress system” (Qiagen), pEGFP, and pET (for this vector, BL21, a strain expressing T7 RNA polymerase, is preferably used as the host).
In addition to the vectors for E. coli, for example, the vector may be a mammal-derived expression vector (e.g., pcDNA3 (Invitrogen), pEGF-BOS, pEF, and pCDM8), an insect cell-derived expression vector (e.g., “Bac-to-BAC baculovairus expression system” (GibcoBRL) and pBacPAK8), a plant-derived expression vector (e.g., pMH1 and pMH2), an animal virus-derived expression vector (e.g., pHSV, pMV, and pAdexLcw), a retrovirus-derived expression vector (e.g., pZIPneo), a yeast-derived expression vector (e.g., “Pichia Expression Kit” (Invitrogen), pNV11, and SP-Q01), or a Bacillus subtilis-derived expression vector (e.g., pPL608 and pKTH50).
In order to express nucleic acid molecules in animal cells, such as CHO, COS, Vero and NIH3T3 cells, the vector should have a promoter necessary for expression in such cells, e.g., SV40 promoter, MMLV-LTR promoter, EF1α promoter, CMV promoter, etc., and more preferably it has a marker gene for selecting transformants (for example, a drug resistance gene selected by a drug (e.g., neomycin, G418, etc.). Examples of vectors with these characteristics include pMAM, pDR2, pBK-RSV, pBK-CMV, pOPRSV and pOPI3.
Nucleic acid molecules comprising a double-stranded region of the present invention can be expressed in animals such as avians by, for example, inserting an open reading frame(s) encoding the nucleic acid into an appropriate vector and introducing the vector by the retrovirus method, liposome method, cationic liposome method, adenovirus method, and so on. The vectors used include, but are not limited to, adenoviral vectors (e.g., pAdexlcw) and retroviral vectors (e.g., pZIPneo). General techniques for gene manipulation, such as insertion of nucleic acids of the invention into a vector, can be performed according to conventional methods.
The present invention also provides a host cell into which an exogenous nucleic acid molecule, typically in a vector of the present invention, has been introduced. The host cell of this invention can be used as, for example, a production system for producing or expressing the nucleic acid molecule. For in vitro production, eukaryotic cells or prokaryotic cells can be used.
Useful eukaryotic host cells may be animal, plant, or fungi cells. As animal cells, mammalian cells such as CHO, COS, 3T3, myeloma, baby hamster kidney (BHK), HeLa, or Vero cells MDCK cells, DF1 cells, amphibian cells such as Xenopus oocytes, or insect cells such as Sf9, Sf21, or Tn5 cells can be used. CHO cells lacking DHFR gene (dhfr-CHO) or CHO K-1 may also be used. The vector can be introduced into the host cell by, for example, the calcium phosphate method, the DEAE-dextran method, cationic liposome DOTAP (Boehringer Mannheim) method, electroporation, lipofection, etc.
Useful prokaryotic cells include bacterial cells, such as E. coli, for example, JM109, DH5α, and HB101, or Bacillus subtilis.
Culture medium such as DMEM, MEM, RPMI-1640, or IMDM may be used for animal cells. The culture medium can be used with or without serum supplement such as fetal calf serum (FCS). The pH of the culture medium is preferably between about 6 and 8. Cells are typically cultured at about 30 to 40° C. for about 15 to 200 hr, and the culture medium may be replaced, aerated, or stirred if necessary.
Compositions
The present invention also provides compositions comprising a nucleic acid molecule comprising a double-stranded region that can be administered to an avian egg. A composition comprising a nucleic acid molecule comprising a double-stranded region may contain a pharmaceutically acceptable carrier to render the composition suitable for administration.
Suitable pharmaceutical carriers, excipients and/or diluents include, but are not limited to, lactose, sucrose, starch powder, talc powder, cellulose esters of alkonoic acids, magnesium stearate, magnesium oxide, crystalline cellulose, methyl cellulose, carboxymethyl cellulose, gelatin, glycerin, sodium alginate, antibacterial agents, antifungal agents, gum arabic, acacia gum, sodium and calcium salts of phosphoric and sulfuric acids, polyvinylpyrrolidone and/or polyvinyl alcohol, saline, and water. In an embodiment, the carrier, excipient and/or diluent is phosphate buffered saline or water.
In an embodiment, the composition may also comprise a transfection promoting agent. Transfection promoting agents used to facilitate the uptake of nucleic acids into a living cell are well known within the art. Reagents enhancing transfection include chemical families of the types; polycations, dendrimers, DEAE Dextran, block copolymers and cationic lipids. Preferably, the transfection-promoting agent is a lipid-containing compound (or formulation), providing a positively charged hydrophilic region and a fatty acyl hydrophobic region enabling self-assembly in aqueous solution into vesicles generally known as micelles or liposomes, as well as lipopolyamines.
In another embodiment, the composition comprises a polymeric biomaterial such as chitosan.
It is understood that any conventional media or agent may be used so long as it is not incompatible with the compositions or methods of the invention.
Administration
Administration of a nucleic acid molecule comprising a double-stranded region (including a composition comprising a nucleic acid molecule comprising a double-stranded region) is conveniently achieved by injection into the egg, and generally injection into the chorion allantoic fluid. Notwithstanding that the air sac is the preferred route of in ovo administration, other regions such as the yolk sac, air sac or amnionic cavity (amnion) may also be inoculated by injection. The hatchability rate might decrease slightly when the air sac is not the target for the administration although not necessarily at commercially unacceptable levels. The mechanism of injection is not critical to the practice of the present invention, although it is preferred that the needle does not cause undue damage to the egg or to the tissues and organs of the developing embryo or the extra-embryonic membranes surrounding the embryo.
Preferably, the nucleic acid molecule is administered within four days of the egg having been laid.
Generally, a hypodermic syringe fitted with an approximately 22 gauge needle is suitable. The method of the present invention is particularly well adapted for use with an automated injection system, such as those described in U.S. Pat. No. 4,903,635, U.S. Pat. No. 5,056,464, U.S. Pat. No. 5,136,979 and US 20060075973.
The nucleic acid molecule is administered in an effective amount sufficient to modify sex in at least some of the eggs which have been administered. The modification can be detected by comparing a suitable number of samples subjected to the method of the invention to a similar number that have not. Statistically significant variation in the sex of the birds between the two groups will be indicative that an effective amount has been administered. Other means of determining an effective amount for sex are well within the capacity of those skilled in the art.
Preferably, about 1 ng to 100 μg, more preferably about 100 ng to 1 μg, of nucleic acid is administered to the egg. Furthermore, it is preferred that the nucleic acid to be administered is in a volume of about 1 μl to 1 ml, more preferably about 10 μl to 500 μl.
EXAMPLES
Example 1
Identification of shRNA Molecules for Down-Regulating DMRT1 Protein Production in Chickens
Selection of shRNA Sequences Targeting DMRT1
The present inventors identified 51 predicted shRNA sequences to target chicken Dmrt1 (Table 4).
There are several algorithms available to select potential siRNA sequences for specific target genes. Taxman et al. (2006) have specifically designed an algorithm to predict effective shRNA molecules and the present inventors have made their own modification to the algorithm to improve shRNA prediction. There are four criteria for shRNA selection using the Taxman algorithm. Three of the criteria are scored for out of a maximum number of 4 points. These criteria are: 1) C or G on the 5′ end of the sequence=1 point, A or T on 5′ end=−1 point; 2) A or T on the 3′ end=1 point, C or G on the 3′ end=−1 point; 3) 5 or more A or T in the seven 3′ bases=2 points, 4 A or T in the seven 3′ bases=1 point. shRNA sequences with the highest scores are preferred. The fourth criteria is based on a calculation for the free-energy of the 6 central bases of the shRNA sequence (bases 6-11 of the sense strand hybridised to bases 9-14 of the antisense strand). shRNAs with a central duplex ΔG>−12.9 kcal/mol are preferred.
The shRNA designer website uses this algorithm to provide a score for each shRNA target. Based on the algorithm and their calculated ΔG value, the present inventors chose 4 of the shRNA target finder shRNA sequences as potentially effective shRNAs to test for their ability to knockdown Dmrt1 gene expression. The selected sequences are shown in their 5′-3′ sequence in Table 5. These 4 sequences were used to construct ddRNAi plasmids for the expression of the 6 shRNAs.
Construction of ddRNAi Plasmids for Expression of Selected shRNAs
To construct the Dmrt1 shRNA expression constructs, two oligonucleotides complementary to each other were designed to contain the sense, loop, antisense and termination signal, followed by a spacer sequence (GGAA) and a BamHI restriction site for screening. In addition, the “bottom oligo (B)” or reverse oligonucleotide contained a SalI overhang at the 5′ end for insertion into the expression vector containing the chicken polymerase III promoter cU6-4 (DQ531570). Table 6 lists the shRNA targets to their corresponding oligonucleotides. The complementary oligonucleotides for each target shRNA, were annealed together and ligated into the PmeI-SalI digested pU6-4 vector. Full length clones were positive if linearised by BamHI digestion. All shRNA expression vectors were sequence confirmed. The four constructs were referred to as 105sh, 240sh, 465sh and 591sh as shown in FIG. 1. The non-silencing control shRNA construct (NSsh) was designed in the same way. However, the sequence used was that of an irrelevant target (ie. NP gene of influenza).
Each ddRNAi plasmid was constructed so that the start of each shRNA sequence was at the +1 position of the native U6 snRNA transcripts. All final shRNA expression vectors consisted of either one of the full length chicken U6 promoters, a shRNA sense sequence, a loop sequence, a shRNA antisense sequence, a termination sequence and a BamHI site. The loop sequence used in all shRNAs was 5′ UUCAAGAGA 3′.
TABLE 4
|
|
Algorithm selection of shRNA sequences targeting
|
Dmrt1.
|
Target Sequence
Start Position
SEQ ID NO
Score
|
|
GGCACAAGCGGTTCTGCAT
79
3435
3
|
|
GACTGCCAGTGCAAGAAGT
105
3436
3
|
|
CTGAGCCAGTTGTCAAGAA
238
3437
3
|
|
GAGCCAGTTGTCAAGAAGA
240
3438
3
|
|
GACGGATGCTCATTCAGGA
355
3439
3
|
|
GCACGTCTGATTTGGTTGT
409
3440
3
|
|
GTGGACTCCACCTACTACA
426
3441
3
|
|
CCAGCCATCCCTGTATCCT
455
3442
3
|
|
CCATCCCTGTATCCTTACT
459
3443
3
|
|
CATCCCTGTATCCTTACTA
460
3444
3
|
|
CCCTGTATCCTTACTATAA
463
3445
4
|
|
CTGTATCCTTACTATAACA
465
3446
4
|
|
CTTACTATAACAACCTGTA
472
3447
3
|
|
CTCCCAGTACCAAATGGCA
497
3448
3
|
|
GCCACTGAGTCTTCCTCAA
519
3449
3
|
|
CTGAGTCTTCCTCAAGTGA
523
3450
3
|
|
GAGTCTTCCTCAAGTGAGA
525
3451
3
|
|
CTCCCAGCAACATACATGT
591
3452
4
|
|
CCCAGCAACATACATGTCA
593
3453
3
|
|
CCAGCAACATACATGTCAA
594
3454
3
|
|
CAGATGAAGGGAATGGAGA
633
3455
3
|
|
CCACCTGCGTCACACAGAT
733
3456
3
|
|
CACCTGCGTCACACAGATA
734
3457
3
|
|
CCTGCGTCACACAGATACT
736
3458
3
|
|
CTCCTACTCAGAGTCGAAA
773
3459
3
|
|
CACTGTTGCCTTGTTCTGT
967
3460
3
|
|
GGTGCCGTGATGTGTTTGT
1189
3461
3
|
|
GTGCCGTGATGTGTTTGTA
1190
3462
4
|
|
GCCGTGATGTGTTTGTAGT
1192
3463
4
|
|
CCTCGTATCGCCAAATTAA
1239
3464
4
|
|
GCCTCGACTTAGATTGCAA
1283
3465
3
|
|
CCTCGACTTAGATTGCAAT
1284
3466
4
|
|
CTCGACTTAGATTGCAATA
1285
3467
4
|
|
CGACTTAGATTGCAATATA
1287
3468
4
|
|
GACTTAGATTGCAATATAA
1288
3469
4
|
|
GCGGCCAGCAAACAAGTCT
1307
3470
3
|
|
GGCCAGCAAACAAGTCTCA
1309
3471
3
|
|
GCCAGCAAACAAGTCTCAA
1310
3472
3
|
|
CCAGCAAACAAGTCTCAAA
1311
3473
3
|
|
GCGTTTCTGCGAGTGTTAT
1342
3474
4
|
|
GTGTCCTCTTCCTGTGTTA
1381
3475
3
|
|
GTCCTCTTCCTGTGTTACA
1383
3476
3
|
|
CCTCTTCCTGTGTTACAGA
1385
3477
3
|
|
CTCTTCCTGTGTTACAGAA
1386
3478
4
|
|
GAAGCCAACCTGAAATGAA
1402
3479
4
|
|
GCCAACCTGAAATGAAACT
1405
3480
4
|
|
CCAACCTGAAATGAAACTA
1406
3481
4
|
|
CCTGAAATGAAACTAGTCT
1410
3482
3
|
|
GTTGCAGCTGTACCTGAAA
1452
3483
3
|
|
GCAGCTGTACCTGAAATAA
1455
3484
4
|
|
CAGCTGTACCTGAAATAAA
1456
3485
4
|
|
TABLE 5
|
|
Sequence of Dmrt1 shRNAs.
|
Target
|
Number
Position
Target Sequence
Score
ΔG
|
|
1
105 bp
GACTGCCAGTGCAAGAAGT
3
−17.1
|
(SEQ ID NO: 3436)
|
|
2
240 bp
GAGCCAGTTGTCAAGAAGA
3
−12.7
|
(SEQ ID NO: 3438)
|
|
3
465 bp
CTGTATCCTTACTATAACA
4
−11.9
|
(SEQ ID NO: 3446)
|
|
4
591 bp
CTCCCAGCAACATACATGT
4
−14.0
|
(SEQ ID NO: 3452)
|
|
Testing Selected shRNAs for Knockdown of Dmrt1 Gene Expression
A reporter gene expression assay was used to test shRNAs for silencing of Dmrt1. The reporter gene was a transcriptional gene fusion of Dmrt1 inserted downstream of the 3′ end of the Enhanced Green Fluorescent Protein (EGFP) gene in pEGFP-C (Clontech). The reporter plasmid was constructed as follows: cDNA of Dmrt1 was reverse transcribed from total RNA isolated from 4 day old embryo's and cloned into the multiple cloning site of pCMV-Script (Stratagene). The Dmrt1 insert was removed from the cloning vector as a NotI-EcoRI fragment and cloned downstream of the EGFP gene in pEGFP-C (Clontech). The resulting plasmid was named pEGFP-Dmrt1. This plasmid was transfected into chicken DF-1 cells and expression of the transcriptional gene fusion was confirmed by measuring EGFP fluorescence using flow cytometry as described below.
Dmrt1 gene silencing assays were conducted by co-transfecting DF-1 cells with the pEGFP-Dmrt1 reporter plasmid and each of the ddRNAi plasmids expressing the Dmrt1 specific and control shRNAs. The co-transfection experiments were performed as follows: DF-1 cells (ATCC CRL-12203, chicken fibroblast) were maintained in a humidified atmosphere containing 5% CO2 at 37° C. in Dulbecco's Modified Eagle's Medium (DMEM) containing 4.5 g/l glucose, 1.5 g/l sodium bicarbonate, 10% foetal calf serum (FCS), 2 mM L-glutamine supplemented with penicillin (100 Um') and streptomycin (100 μg/ml). DF1 cells were passaged as required using 0.25% (w/v) trypsin-ethylenediaminetetraacetic acid (EDTA).
TABLE 6
|
|
Sequence and details of primers used.
|
|
|
Target
|
Number
Position
Target Sequence
Score
ΔG
|
|
1
105 bp
GACTGCCAGTGCAAGAAGT
3
−17.1
|
(SEQ ID NO: 3436)
|
DMRT1-105T-U6.4
|
GACTGCCAGTGCAAGAAGTTTCAAGAGAACTTCTTGCACTGGCAGTCTTT
|
TTGGAAGGATCC (SEQ ID NO: 3486)
|
DMRT1-105B-U6.4
|
TCGA
GGATCC
TTCCAAAAAGACTGCCAGTGCAAGAAGTTCTCTTGAAAC
|
TTCTTGCACTGGCAGTC (SEQ ID NO: 3487)
|
|
Target
|
Number
Position
Target Sequence
Score
ΔG
|
|
2
240 bp
GAGCCAGTTGTCAAGAAGA
3
−12.7
|
(SEQ ID NO: 3438)
|
DMRT1-240T-U6.4
|
GAGCCAGTTGTCAAGAAGATTCAAGAGATCTTCTTGACAACTGGCTCTTT
|
TTGGAAGGATCC (SEQ ID NO: 3488)
|
DMRT1-240B-U6.4
|
TCGA
GGATCC
TTCCAAAAAGAGCCAGTTGTCAAGAAGATCTCTTGAATCT
|
TCTTGACAACTGGCTC (SEQ ID NO: 3489)
|
|
Target
|
Number
Position
Target Sequence
Score
ΔG
|
|
3
465 bp
CTGTATCCTTACTATAACA
4
−11.9
|
(SEQ ID NO: 3446)
|
DMRT1-465T-U6.4
|
CTGTATCCTTACTATAACATTCAAGAGATGTTATAGTAAGGATACAGTTT
|
TTGGAAGGATCC (SEQ ID NO: 3490)
|
DMRT1-465B-U6.4
|
TCGA
GGATCC
TTCCAAAAACTGTATCCTTACTATAACATCTCTTGAATGT
|
TATAGTAAGGATACAG (SEQ ID NO: 3491)
|
|
Target
|
Number
Position
Target Sequence
Score
ΔG
|
|
4
591 bp
CTCCCAGCAACATACATGT
4
−14.0
|
(SEQ ID NO: 3452)
|
DMRT1-591T-U6.4
|
CTCCCAGCAACATACATGTTTCAAGAGAACATGTATGTTGCTGGGAGTTT
|
TTGGAAGGATCC (SEQ ID NO: 3492)
|
DMRT1-591B-U6.4
|
TCGA
GGATCC
TTCCAAAAACTCCCAGCAACATACATGTTCTCTTGAAACA
|
TGTATGTTGCTGGGAG (SEQ ID NO: 3493)
|
|
Bold = spacer sequence;
|
Italics = BamHI restriction site;
|
Underline = SalI overhang
|
Co-transfection of pEGFP-Dmrt1 and ddRNAi plasmids for EGFP-Dmrt1 fusion silencing assays was conducted in DF-1 cells grown to 80-90% confluence, in 24 well culture plates (Nunc) for flow cytometry analysis. Cells were transfected with a total of 1 μg of plasmid DNA, per well, using Lipofectamine™2000 transfection reagent (Invitrogen). EGFP expression was analysed in transfected DF-1 cells at 60 hours post-transfection using flow cytometry analysis of transfections performed in triplicate. Cells were trypsinised using 100 μl of 0.25% trypsin-EDTA, pelleted at 2000 rpm for 5 minutes, washed once in 1 ml of cold phosphate buffered saline-A (PBSA) (Oxoid), twice in 1 ml of FACS-wash solution (PBSA+1% FCS) and resuspended in 250 μl of FACS-wash solution. Flow cytometry sampling was performed using a FACScalibur (Becton Dickinson) fluorescence activated cell sorter. Data acquisition and calculation of mean fluorescence intensity (MFI) values for triplicate co-transfection samples, was performed using CELLQuest software (Becton Dickinson). The results of the gene silencing assay are shown in FIG. 1. Compared to the negative control irrelevant shRNA expressed from NSsh, the Dmrt1 specific shRNAs were observed to knockdown expression of the reporter gene to varying levels. Dmrt1 shRNA 240sh induced the greatest level of gene silencing of approximately 60%.
Example 2
In Ovo Modulation of DMRT1 Gene Expression in Chickens
An siRNA targeted to a conserved exon of the chicken DMRT1 gene was designed using the Ambion siRNA Target Finder tool (www.ambion.com). The chosen siRNA was designated DMRT1-343-siRNA (5′-GAGCCAGUUGUCAAGAAGAUU-3′) (SEQ ID NO:3431). The siRNA was synthesized and obtained from Qiagen.
For in ovo delivery, the siRNA was formulated with lipoefectamine 2000 (Invitrogen) according to the manufacturer's instructions. The now complexed siRNA was then delivered in ovo at a dose of either 100 μmol or 200 μmol. The siRNA was injected into embryonated eggs via an intravenous (I.V.) route or directly into the amnion at embryonic day 4.5 (E4.5). For both I.V. and amnion delivery, a small opening (1 cm×1 cm) was created at the top of the blunt end of the egg so as to avoid the membrane, veins and arteries, and 100 pmol or 200 pmol in a 4 μl volume was then injected directly into a vein or into the amnionic cavity using a micro-capillary pipette. Micro-capillaries of 1 mm diameter were used for injections, and their tips were pulled to a diameter of 40 microns with bevelled tip of 22.5°. After injection, the holes in the eggs were sealed with appropriate sized parafilm squares using a heated scalpel blade.
In total, 286 embryonated eggs (E4.5) were used in this experiment;
Group 1: 48 eggs were used as controls and were not injected with the DMRT1-343-siRNA formulation;
Group 2: 51 eggs were injected I.V. with 100 pmol of siRNA;
Group 3: 53 eggs were injected I.V. with 200 pmol of siRNA;
Group 4: 81 eggs were injected into the amnion with 100 pmol of siRNA and;
Group 5: 53 eggs were injected into the amnion with 200 pmol of siRNA.
All embryos were incubated until day E10. At E10, all embryos were assessed for viability and then removed from the egg. Control Group 1 had an embryo viability of 100%; Group 2 had a viability of 76%; Group 3 had a viability of 94%; Group 4 had a viability of 40% and; Group 5 had a viability of 75%. A single limb bud from each embryo was removed and used in a sex determination PCR test to determine if the embryos were of male or female genotype. Lower limb buds from each embryo were collected into 50 μl of PCR digestion buffer (50 mM KCl; 10 mM Tris-HCl, pH8.3; 0.1 mg/ml gelatine; 0.45% Nonidet P-40; 0.45% Tween-20; 0.2 mg/ml proteinase K; stock stored at −20° C.) at room temperature and digested at 55° C. for a minimum of 1 h, then at 95° C. for 10 min to release genomic DNA.
Sexing was carried out by PCR using the method of Clinton et al. (2001). The PCR mix consisted of 1 μl of digestion mix, 10×RedTaq reaction buffer (Sigma-Aldrich), MgCl2 to 1.5 mM (Promega), 1 unit of RedTaq DNA polymerase (Sigma-Aldrich) and Milli-Q water (Millipore) to a total volume of 20 μl. Reactions were carried out in a Master cycler S (Eppendorf) PCR machine. Products were run on a 1.5% 1×Tris-borate (TBE) agarose gels.
Once the sex PCR test was complete and analysed, the embryos were definitively labelled as either being genotypically male or female. The embryos were then opened via dissection and the gonads exposed for macroscopic analysis of gonadal development. The gonadal development of all control embryos was normal as expected. Control female embryo's showed typical asymmetric development that was characterised by a large left ovary and smaller regressing right gonad. Control male embryos all had typical bilateral testes. All female embryos from the siRNA knockdown groups (Groups 2-5) had normal gonadal development. In contrast, some male embryos from the siRNA knockdown groups showed varying degrees of female-like asymmetry at the macroscopic level of the gonads. The feminisation effect of the DMRT1-343-siRNA was characterised by an average or small-sized right testis and a larger feminised left gonad (Table 7). Feminisation was observed in a number of male embryos in Groups 2, 3 and 5 and resulted in an increase in the ratio of embryos with female-like gonads in these groups.
TABLE 7
|
|
DMRT1 embryo injection results.
|
%
|
Male:Female
|
siRNA dose
No.
% Male:Female
Macroscopic
|
and injection
embryos
No. viable at
Genotype
Gonad
|
route
injected
E10
(PCR sex test)
phenotype
|
|
Group 1
48
48 (100%)
60:40
60:40
|
No injection
|
control
|
Group 2
51
39 (76%)
59:41
54:46
|
I.V.—100 pmol
|
Group 3
53
50 (94%)
54:46
46:54
|
I.V.—200 pmol
|
Group 4
81
33 (40%)
48:52
48:52
|
Amnion—100
|
pmol
|
Group 5
53
40 (75%)
33:67
28:72
|
Amnion—200
|
pmol
|
|
Gonads from both male and female embryos in each treatment groups were assessed for DMRT1 gene expression using quantitative RT-PCR analysis. Both the female and male gonads were pooled separately from each group and RNA was extracted for cDNA synthesis and qPCR analysis. The pooled gonads were added to 1 ml of Trizol and homogenised well by pipetting and vortexing at room temperature until all gonad tissue had dissolved. 200 μl of chloroform was added and mixed well by inverting the sample for 15 sec. The sample was then incubated at room temperature for 3 min and then centrifuged at 12000 g for 15 min at 4° C. The aqueous phase of the sample was then transferred to a new tube and then 500 l of isopropanol was added and mixed well by inversion. The mix was then incubated at room temperature for 10 min and then centrifuged at 12000 g for 10 min at 4° C. The supernatant was removed from the tube carefully, so as not to disturb the RNA pellet, and the pellet was then washed with 1 ml of 70% ethanol. The tube was then centrifuged at 7500 g for 5 min at 4° C. and the supernatant again was carefully removed and the RNA pellet was air dried at room temperature for 10 min. The RNA pellet was then resuspended in 25 μl of RNase-free water and the final concentration of RNA was determined using a NanoDrop ND-1000 Spectrophotometer (Thermo Scientific). RNA was reverse transcribed to complimentary DNA (cDNA) using the Promega Reverse Transcription kit (Promega). The reaction mix contained 1 μg of RNA, random hexamers (1 μl), dNTPs (2 μl), AMV reverse transcriptase (Promega) (0.5 μl) and nuclease free water added to a total reaction volume of 20 μl. The mix was incubated at 42° C. for 1 hour, followed by a 10 min incubation at 95° C. for enzyme inactivation.
cDNA was then used to quantify relative DMRT1 gene expression levels in the pooled male and female gonad samples from each treatment group. qPCR primers and probes were designed using Primer Express (Applied Biosystems) software and sequences are shown in Table 8. PCR's were set up in 20 μl reaction volumes that contained 2×TaqMan qRT PCR mastermix (Applied Biosystems), 1 μl of primer/probe mix, 1 μl of cDNA sample and made up to final volume with Nuclease free water (Promega). PCR cycling was performed at 95° C. for 1 min, followed by 40 cycles of 95° C. for 15 sec; 61° C. for 30 sec and; 68° C. for 30 sec. Ct values were obtained at a standard threshold value of 0.2 for all reactions. This threshold value corresponded to the midway point of the logarithmic phase of all amplification plots. Ct values were exported to Microsoft Excel for analysing relative gene expression using the comparative Ct method.
TABLE 8
|
|
Primer and probe sequences.
|
Sequence Name
Sequence 5′-3′
|
|
DMRT1 TaqMan MGB probe
CCATCCCTTTCATCTGCC
|
(SEQ ID NO: 3494)
|
DMRT1 Forward primer
TCAAGCCAGTCAGGAAAACAGT
|
(SEQ ID NO: 3495)
|
DMRT1 Reverse primer
TCATGGCATGGCGGTTCT
|
(SEQ ID NO: 3496)
|
18S rRNA TaqMan MGB probe
TGCTGGCACCAGACTTGCCCTC
|
(SEQ ID NO: 3497)
|
18S rRNA Forward primer
CGGCTACCACATCCAAGGAA
|
(SEQ ID NO: 3498)
|
18S rRNA Reverse primer
GCTGGAATTACCGCGGCT
|
(SEQ ID NO: 3499)
|
|
Relative levels of DMRT1 mRNA were compared with the chicken house keeping 18S rRNA species across all cDNA samples (FIG. 2). Quantitative RT-PCR analysis confirmed that DMRT1 mRNA expression was specifically reduced in all pooled groups of male embryos when compare to control Group 1. Almost 40% of DMRT1 gene expression knockdown was observed for Group 3 male embryos treated with the DMRT1-343-siRNA. It is interesting to note that Group 3 was also the group that resulted in the greatest degree of observed feminisation of male gonads at the macroscopic level.
It will be appreciated by persons skilled in the art that numerous variations and/or modifications may be made to the invention as shown in the specific embodiments without departing from the spirit or scope of the invention as broadly described. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive.
All publications discussed and/or referenced herein are incorporated herein in their entirety.
The present application claims priority from U.S. 61/138,235 filed 17 Dec. 2008, the entire contents of which are incorporated by reference.
Any discussion of documents, acts, materials, devices, articles or the like which has been included in the present specification is solely for the purpose of providing a context for the present invention. It is not to be taken as an admission that any or all of these matters form part of the prior art base or were common general knowledge in the field relevant to the present invention as it existed before the priority date of each claim of this application.
REFERENCES
- Clinton et al. (2001) British Poultry Science 42:134-138
- Hori et al. (2000) Mol Biol Cell 11:3645-3660
- Needleman and Wunsch (1970) J Mol Biol 48: 443-453
- O'Neill et al. (2000) Dev Genes Evol 210:243-249
- Raymond et al. (1999) Dev Biol 215:208-220
- Smith et al. (1999) Nature 402:601-602
- Smith et al. (2000) Nature 407: 319-320.
- Taxman et al. (2006) BMC Biotechnol 6:7
- Waterhouse et al. (1998) Proc Natl Acad Sci USA 95:13959-13964