Claims
- 1. A method of encapsulating a bioactive material, comprising:
a. dissolving albumin in water; b. solubilizing antisense oligonucleotides (oligomers) to NF-kB in phosphate buffered saline (PBS); c. mixing said dissolved albumin and said solubilized antisense oligonucleotides (oligomers) to NF-kB together; d. cooling the mixture formed in step c.; e. providing a solvent; f. cooling said solvent of step e.; g. maintaining said solvent of step f. at a cooled temperature to form a solvent system; h. nebulizing said dissolved albumin and said solubilized antisense oligonucleotides (oligomers) to NF-kB into said solvent while said solvent of step g. is stirred; i. evaluating said solvent system containing the microencapsulated albumin-drug microspheres of step h. for size to obtain microspheres; j. crosslinking said microspheres with glutaraldehyde while stirring and maintaining said solvent system at a cooled temperature; k. washing the microspheres of step j. with a solvent; l. sizing the microspheres of step k.; and, m. freeze drying the microspheres of step 1.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application is a continuation-in-part of co-pending application U.S. application Ser. No. ______, filed Jun. 13, 2002, entitled “MICROENCAPSULATED COMPOUNDS AND METHOD OF PREPARING SAME”, which is a continuation of application Ser. No. 08/434,542, filed May 4, 1995 (now abandoned), which is a continuation-in-part of application Ser. No. 07/977,057, filed Nov. 16, 1992 (now abandoned), all of which are commonly assigned to the assignee of the present application. The disclosures of all these applications are incorporated by reference in their entirety herein.