MicroRNA1 therapies

Abstract
Delta protein expression in a cell is reduced by introducing miR-1 into the cell, and detecting a resultant reduction of Delta protein expression in the cell.
Description
Claims
  • 1. A method of reducing Delta protein expression in a mammalian cell, comprising the steps of: introducing into the cell miR-1 anddetecting a resultant reduction of Delta protein expression in the cell.
  • 2. The method of claim 1 wherein the Delta protein is predetermined to be overexpressed or upregulated in the cell.
  • 3. The method of claim 1 wherein the cell is selected from a myoblast, an endothelial cell, and a lymphoblast.
  • 4. The method of claim 1 wherein the Delta protein is selected from Delta-like-1 (Dll1), Delta-like-3 (Dll3), and Delta-like-4 (Dll4).
  • 5. The method of claim 1 wherein the Delta protein is Delta-like-4 (Dll4).
  • 6. The method of claim 1 wherein the cell is of a cancer wherein the Delta protein is predetermined to be upregulated.
  • 7. The method of claim 1 wherein the cell is of a cancer wherein the Delta protein is predetermined to be upregulated, the cancer selected from the group consisting a renal cell carcinoma, glioma and cervical cancer.
  • 8. The method of claim 1 wherein the resultant reduction of Delta protein in the cell is detected directly by Western blot or ELISA.
  • 9. The method of claim 1 wherein the resultant reduction of Delta protein in the cell is detected indirectly by detecting a change in Delta-Notch binding or signaling.
  • 10. The method of claim 1 wherein the resultant reduction of Delta protein in the cell is detected by detecting a change in cell proliferation or differentiation pre-correlated with Delta protein expression.
  • 11. The method of claim 1 wherein the resultant reduction is quantitatively detected.
  • 12. The method of claim 1 wherein: the cell is selected from a myoblast, an endothelial cell, and a lymphoblast;the Delta protein is predetermined to be overexpressed or upregulated in the cell; andthe Delta protein is selected from Delta-like-1 (Dll1), Delta-like-3 (Dll3), and Delta-like-4 (Dll4).
  • 13. The method of claim 1 wherein: the cell is of a cancer wherein the Delta protein is predetermined to be upregulated; andthe Delta protein is selected from Delta-like-1 (Dll1), Delta-like-3 (Dll3), and Delta-like-4 (Dll4).
  • 14. The method of claim 13 wherein the resultant reduction of Delta protein in the cell is detected by detecting a change in cell proliferation or differentiation pre-correlated with Delta protein expression.
  • 15. The method of claim 13 wherein the resultant reduction is quantitatively detected.
  • 16. The method of claim 1 wherein: the cell is of a cancer wherein the Delta protein is predetermined to be upregulated; andthe Delta protein is Delta-like-4 (Dll4).
  • 17. The method of claim 16 wherein the resultant reduction of Delta protein in the cell is detected by detecting a change in cell proliferation or differentiation pre-correlated with Delta protein expression.
  • 18. The method of claim 16 wherein the resultant reduction is quantitatively detected.