Information
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Patent Application
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20070142313
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Publication Number
20070142313
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Date Filed
December 15, 200519 years ago
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Date Published
June 21, 200717 years ago
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Inventors
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Original Assignees
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CPC
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US Classifications
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International Classifications
Abstract
Delta protein expression in a cell is reduced by introducing miR-1 into the cell, and detecting a resultant reduction of Delta protein expression in the cell.
Claims
- 1. A method of reducing Delta protein expression in a mammalian cell, comprising the steps of:
introducing into the cell miR-1 anddetecting a resultant reduction of Delta protein expression in the cell.
- 2. The method of claim 1 wherein the Delta protein is predetermined to be overexpressed or upregulated in the cell.
- 3. The method of claim 1 wherein the cell is selected from a myoblast, an endothelial cell, and a lymphoblast.
- 4. The method of claim 1 wherein the Delta protein is selected from Delta-like-1 (Dll1), Delta-like-3 (Dll3), and Delta-like-4 (Dll4).
- 5. The method of claim 1 wherein the Delta protein is Delta-like-4 (Dll4).
- 6. The method of claim 1 wherein the cell is of a cancer wherein the Delta protein is predetermined to be upregulated.
- 7. The method of claim 1 wherein the cell is of a cancer wherein the Delta protein is predetermined to be upregulated, the cancer selected from the group consisting a renal cell carcinoma, glioma and cervical cancer.
- 8. The method of claim 1 wherein the resultant reduction of Delta protein in the cell is detected directly by Western blot or ELISA.
- 9. The method of claim 1 wherein the resultant reduction of Delta protein in the cell is detected indirectly by detecting a change in Delta-Notch binding or signaling.
- 10. The method of claim 1 wherein the resultant reduction of Delta protein in the cell is detected by detecting a change in cell proliferation or differentiation pre-correlated with Delta protein expression.
- 11. The method of claim 1 wherein the resultant reduction is quantitatively detected.
- 12. The method of claim 1 wherein:
the cell is selected from a myoblast, an endothelial cell, and a lymphoblast;the Delta protein is predetermined to be overexpressed or upregulated in the cell; andthe Delta protein is selected from Delta-like-1 (Dll1), Delta-like-3 (Dll3), and Delta-like-4 (Dll4).
- 13. The method of claim 1 wherein:
the cell is of a cancer wherein the Delta protein is predetermined to be upregulated; andthe Delta protein is selected from Delta-like-1 (Dll1), Delta-like-3 (Dll3), and Delta-like-4 (Dll4).
- 14. The method of claim 13 wherein the resultant reduction of Delta protein in the cell is detected by detecting a change in cell proliferation or differentiation pre-correlated with Delta protein expression.
- 15. The method of claim 13 wherein the resultant reduction is quantitatively detected.
- 16. The method of claim 1 wherein:
the cell is of a cancer wherein the Delta protein is predetermined to be upregulated; andthe Delta protein is Delta-like-4 (Dll4).
- 17. The method of claim 16 wherein the resultant reduction of Delta protein in the cell is detected by detecting a change in cell proliferation or differentiation pre-correlated with Delta protein expression.
- 18. The method of claim 16 wherein the resultant reduction is quantitatively detected.