Claims
- 1. A method of identifying a nucleic acid molecule associated with a pathological disorder selected from the group consisting of:
a) contacting a sample comprising nucleic acid molecules with a hybridization probe comprising at least 25 contiguous nucleotides of SEQ ID NO:1 OR 3; and detecting the presence of a nucleic acid molecule in said sample that hybridizes to said probe, and b) contacting a sample comprising nucleic acid molecules with a first and a second amplification primer, said first primer comprising at least 25 contiguous nucleotides of SEQ ID NO:1 OR 3, and said second primer comprising at least 25 contiguous nucleotides from the complement of SEQ ID NO:1 OR 3,; incubating said sample under conditions that allow nucleic acid amplification; and detecting the presence of a nucleic acid molecule in said sample that is amplified, thereby identifying a nucleic acid molecule associated with a pathological disorder.
- 2. A method of identifying a polypeptide associated with a pathological disorder comprising:
a) contacting a sample comprising polypeptides with a 4460 binding substance; and b) detecting the presence of a polypeptide in said sample that binds to said 4460 binding substance, thereby identifying a polypeptide associated with a pathological disorder.
- 3. A method of identifying a subject having a pathological disorder, or at risk for developing a pathological disorder selected from the group consisting of:
a) contacting a sample obtained from said subject comprising nucleic acid molecules with a hybridization probe comprising at least 25 contiguous nucleotides of SEQ ID NO:1 OR 3; and detecting the presence of a nucleic acid molecule in said sample that hybridizes to said probe; and b) contacting a sample obtained from said subject comprising nucleic acid molecules with a first and a second amplification primer, said first primer comprising at least 25 contiguous nucleotides of SEQ ID NO:1 OR 3, and said second primer comprising at least 25 contiguous nucleotides from the complement of SEQ ID NO:1 OR 3,; incubating said sample under conditions that allow nucleic acid amplification; and detecting the presence of a nucleic acid molecule in said sample that is amplified; thereby identifying a subject having a pathological disorder, or at risk for developing a pathological disorder.
- 4. A method of identifying a subject having a pathological disorder, or at risk for developing a pathological disorder comprising:
a) contacting a sample obtained from said subject comprising polypeptides with a 4460 binding substance; and b) detecting the presence of a polypeptide in said sample that binds to said 4460 binding substance, thereby identifying a subject having a pathological disorder, or at risk for developing a pathological disorder.
- 5. A method for treating a subject having a pathological disorder or a pathological disorder characterized by aberrant 4460 polypeptide activity or aberrant 4460 nucleic acid expression comprising administering to the subject a 4460 modulator, thereby treating said subject having a pathological disorder.
- 6. The method of claim 5 wherein the modulator is selected from the group consisting of small organic molecules, peptides, polynucleotides and antibodies.
- 7. The method of any of claims 1-5 wherein the pathological disorder is a cardiovascular disorder.
- 8. A method of detecting an agent that binds a 4460 polypeptide comprising:
a) combining an agent selected from the group consisting of small organic molecules, peptides, polynucleotides and antibodies; and b) detecting or measuring the formation of a complex between the agent and the 4460 polypeptide under conditions suitable for binding of an agent to the 4460 polypeptide, to thereby identify an agent that binds to the 4460 polypeptide.
- 9. The method of claim 7 wherein the agent is a modulator of 4460 polypeptide activity.
RELATED APPLICATIONS
[0001] This application claims priority to U.S. provisional application No. 60/323,018, filed on Sep. 18, 2001, the contents of which are incorporated herein by reference.
Provisional Applications (1)
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Number |
Date |
Country |
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60323018 |
Sep 2001 |
US |