Claims
- 1. A mixture comprising an isolated first polypeptide and a second polypeptide, said first polypeptide comprising at least one sequence selected from the group consisting of SEQ ID NOS:2-14, or a subsequence thereof having at least 16 consecutive amino acid residues thereof, said second polypeptide comprising at least one sequence selected from the group consisting of SEQ ID NOS:15-20, or a subsequence thereof sufficient to specifically bind said first polypeptide.
- 2. A mixture according to claim 1, the first polypeptide comprising at least one sequence selected from the group consisting of SEQ ID NOS:2-14, or a subsequence thereof having at least 64 consecutive amino acid residues thereof.
- 3. A mixture according to claim 1, the first polypeptide comprising at least one sequence selected from the group consisting of SEQ ID NOS:2-14.
- 4. A mixture according to claim 1, the first polypeptide comprising SEQ ID NO:2, or a subsequence thereof having at least 16 consecutive amino acid residues thereof.
- 5. A mixture according to claim 1, the first polypeptide comprising SEQ ID NO:2, or a subsequence thereof having at least 64 consecutive amino acid residues thereof.
- 6. A mixture according to claim 1, the first polypeptide comprising at least one sequence selected from the group consisting of SEQ ID NOS:3-6, or a subsequence thereof having at least 16 consecutive amino acid residues thereof.
- 7. A mixture according to claim 1, the first polypeptide comprising at least one sequence selected from the group consisting of SEQ ID NOS:3-6, or a subsequence thereof having at least 64 consecutive amino acid residues thereof.
- 8. A mixture according to claim 1, the first polypeptide comprising SEQ ID NO:7, or a subsequence thereof having at least 16 consecutive amino acid residues thereof.
- 9. A mixture according to claim 1, the first polypeptide comprising SEQ ID NO:7, or a subsequence thereof having at least 64 consecutive amino acid residues thereof.
- 10. A mixture according to claim 1, the first polypeptide at comprising least one sequence selected from the group consisting of SEQ ID NOS:8-9, or a subsequence thereof having at least 16 consecutive amino acid residues thereof.
- 11. A mixture according to claim 1, the first polypeptide comprising at least one sequence selected from the group consisting of SEQ ID NOS:8-9, or a subsequence thereof having at least 64 consecutive amino acid residues thereof.
- 12. A mixture according to claim 1, the first polypeptide comprising at least one sequence selected from the group consisting of SEQ ID NOS:10-11, or a subsequence thereof having at least 16 consecutive amino acid residues thereof.
- 13. A mixture according to claim 1, the first polypeptide comprising at least one sequence selected from the group consisting of SEQ ID NOS: 10-11, or a subsequence thereof having at least 64 consecutive amino acid residues thereof.
- 14. A mixture according to claim 1, the first polypeptide comprising at least one sequence selected from the group consisting of SEQ ID NOS: 12-14, or a subsequence thereof having at least 16 consecutive amino acid residues thereof.
- 15. A mixture according to claim 1, the first polypeptide comprising at least one sequence selected from the group consisting of SEQ ID NOS: 12-14, or a subsequence thereof having at least 64 consecutive amino acid residues thereof.
- 16. A mixture according to claim 1, the first polypeptide comprising at least one sequence selected from the group consisting of SEQ ID NO:2, amino acid residues 1-10; SEQ ID NO:2, amino acid residues 29-41; SEQ ID NO:2, amino acid residues 75-87; SEQ ID NO:2, amino acid residues 92-109; SEQ ID NO:2, amino acid residues 132-141; SEQ ID NO:2, amino acid residues 192-205; SEQ ID NO:2, amino acid residues 258-269; SEQ ID NO:2, amino acid residues 295-311; SEQ ID NO:2, amino acid residues 316-330; SEQ ID NO:2, amino acid residues 373-382; SEQ ID NO:2, amino acid residues 403-422; SEQ ID NO:2, amino acid residues 474-485; SEQ ID NO:2, amino acid residues 561-576; SEQ ID NO:2, amino acid residues 683-697; SEQ ID NO:2, amino acid residues 768-777; SEQ ID NO:2, amino acid residues 798-813; SEQ ID NO:2, amino acid residues 882-894; SEQ ID NO:2, amino acid residues 934-946; SEQ ID NO:2, amino acid residues 1054-1067; SEQ ID NO:2, amino acid residues 1181-1192; SEQ ID NO:2, amino acid residues 1273-1299; SEQ ID NO:2, amino acid residues 1383-1397; SEQ ID NO:2, amino acid residues 1468-1477; and SEQ ID NO:2, amino acid residues 1508-1517.
- 17. A mixture according to claim 1, comprising a cell comprising the Second polypeptide.
- 18. A mixture according to claim 3, comprising a cell comprising the Second polypeptide.
- 19. A mixture according to claim 1, comprising a candidate agent for modulating an interaction of the Robo and Slit polypeptides.
- 20. A method of identifying agents which modulate the interaction of a Second polypeptide and a Slit polypeptide, said method comprising the steps of:combining the mixture of claim 1 and a candidate agent under conditions whereby, but for the presence of the agent, the Second and first polypeptides engage in a first interaction, and determining a second interaction of the Second and first polypeptides in the presence of the agent, wherein a difference between the first and second interactions indicates that the agent modulates the interaction of the Robo and Slit polypeptides.
- 21. A method of identifying agents which modulate the interaction of a Second polypeptide and a Slit polypeptide, said method comprising the steps of:combining the mixture of claim 3 and a candidate agent under conditions whereby, but for the presence of the agent, the Second and first polypeptides engage in a first interaction, and determining a second interaction of the Second and first polypeptides in the presence of the agent, wherein a difference between the first and second interactions indicates that the agent modulates the interaction of the Robo and Slit polypeptides.
CROSS-REFERENCE TO RELATED APPLICATION
This application is a Divisional of U.S. application Ser. No. 09/191,647, filed Nov. 13, 1998 U.S. Pat. No. 6,046,015, which claims the benefit of U.S. Provisional Application No. 60/081,057 filed Apr. 07, 1998 and U.S. Provisional Application No. 60/065,544, filed Nov. 14, 1997.
Government Interests
The research carried out in the subject application was supported in part by NIH grant NS18366. The government may have rights in any patent issuing on this application.
Non-Patent Literature Citations (1)
Entry |
Wilson et al., Nature, vol. 368: pp. 32-38. (Mar. 3, 1994). |
Provisional Applications (2)
|
Number |
Date |
Country |
|
60/081057 |
Apr 1998 |
US |
|
60/065544 |
Nov 1997 |
US |