Claims
- 1. A hybrid cell line derived by hybridoma technique which produces a monoclonal antibody that binds to a common antigen comprised of a 110,000 dalton molecular weight glycoprotein on human CD4 and CD8 lymphocytes, which antigen, present on helper inducer cells but not suppressor inducer cells, enables the monoclonal antibody to distinguish between helper inducer and suppressor inducer cells in human CD4 lymphocyte populations.
- 2. The cell line according to claim 1 in which monoclonal antibody also binds to said common antigen on B cells and macrophages.
- 3. The hybrid cell line according to claim 1 which has the identifying characteristics of the cell line on deposit with the A.T.C.C., Rockville, Maryland and assigned A.T.C.C. deposit No. HB 10297.
- 4. A hybrid cell line derived by hybridoma technique which produces a monoclonal antibody which binds specifically to an antigen identified as the lF7 antigen on human CD4 helper inducer lymphocytes and which enables the monoclonal antibody to distinguish between helper inducer and suppressor inducer cells in human CD4 lymphocyte populations.
- 5. The cell line according to claim 4 which produces mouse IgGl isotype antibody to lF7 antigen.
- 6. A monoclonal antibody which specially binds to a common antigen identified as the lF7antigen comprised of a 110,000 dalton molecular weight glycoprotein on the surface of T lymphocyte cell populations inhuman blood comprised of CD4 and CD8 lymphocytes, said monoclonal antibody also binding to B cells and macrophages.
- 7. The monoclonal antibody of claim 6 which is produced by the cell line having the identifying characteristics of the cell line samples on deposit with the American Type Culture Collection and assigned A.T.C.C. No. HB 10297.
- 8. A monoclonal antibody which specifically binds to an antigen on the surface of T lymphocyte populations in human blood, said antigen being comprised of a 110,000 dalton molecular weight glycoprotein and being detectible on CD4 helpers inducer cells, but essentially undetectible on CD4 suppressor inducer cells.
- 9. A method of distinguishing between helper inducer and suppressor inducer cells in a sample of human CD4 lymphocyte populations comprising, contacting said sample with a monoclonal antibody identified as the lF7 monoclonal antibody for a time and under conditions sufficient for the formation of immunological complexes between said lF7 monoclonal antibody and CD4 helper inducer cells and then detecting said immunological complexes resulting from said contact between said monoclonal antibody and said cells in said sample, said cells complexed with said monoclonal antibody being CD4 helper inducer cells.
- 10. The method of claim 9 including the step of labelling said monoclonal antibody with a detectible compound prior to contacting said sample with said monoclonal antibody such that said complexes are labelled with said detectible compound as said complexes, if any, are formed upon contacting of said sample with said labelled monoclonal antibody.
- 11. The method of claim 9 including the step of separating the detected immunological complexes by cell sorting flow cytometric procedures.
- 12. The method of claim 10 in which said detectible compound is a fluorescent compound.
- 13. The method of claim 10 in which said detectible compound is an enzyme capable of producing a substrate reaction detectible compound.
- 14. A murine monoclonal antibody of the mouse IgGl isotype which is specific for an antigen identified as the lF7 antigen.
- 15. The monoclonal antibody of claim 14 in detectible labelled form.
- 16. The monoclonal antibody of claim 15 wherein said label is selected from the group consisting of a dye, a radioactive element and an electron dense element.
- 17. The monoclonal antibody of claim 14 attached to a compound selected from the group consisting of chemotherapeutic, photoactivated-toxic and radioactive agents.
- 18. A method of detecting and measuring lF7 antigen in liquid biological samples of subsets of CD4 inducer lymphocytes comprising:
- (a) contacting a liquid biological sample with lF7 monoclonal antibody conjugated with a detector group selected from the group consisting of a fluorescent compound, a radioactive element and an enzyme;
- (b) detecting and measuring the lF7 antigen/antibody complex formed, if any, by use of a detecting and measuring means appropriate to the detector group selected, and
- (c) determining the lf7 present in said biological sample as the result of the detection and measurement of the complex of step (b).
- 19. The method of claim 18 including the step of flow cytometic cell sorting of an CD4 inducer lymphocytes bound by said conjugated monoclonal antibody.
- 20. A hybridoma cell line which produces a monoclonal antibody that binds to the antigen identified by the monoclonal antibody produced by the hybridoma cell line having A.T.C.C. Deposit No. HB 10297.
- 21. A monoclonal antibody which binds to the antigen identified by the monoclonal antibody produced by the hybridoma cell line having A.T.C.C. Deposit No. HB 10297.
Government Interests
This invention was made with Government support in part by National Institutes of Health grant Nos. Al-12069, Al-20729, CA25369 and AR 33716.
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