Claims
- 1. A method of forming a proteinaceous material in the form of a polymerized aggregated conjugate protein, which comprises:
- constructing a heterologous plasmid expression vector comprising a first nucleotide sequence coding for the coat protein of tobacco mosaic virus, a second nucleotide sequence coding for a peptide containing an epitope from a pathogen, wherein said first and second nucleotide sequences form an open reading frame, and a promoter operatively coupled to said reading frame to direct expression of a fusion protein encoded by said open reading frame,
- introducing the plasmid expression vector into E. coli,
- growing the E. coli containing the expression vector to express the fusion protein,
- separating the fusion protein from the E. coli to provide a solution of fusion protein,
- modifying the pH of the solution to a pH of 5.0 at which said fusion protein forms a polymerized aggregated conjugate protein of tobacco mosaic virus and at least one epitope, and isolating the polymerized aggregated conjugate protein.
Priority Claims (1)
Number |
Date |
Country |
Kind |
8421282 |
Aug 1984 |
GBX |
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Parent Case Info
This is a division of application Ser. No. 08/187,135 filed Jan. 27, 1994 now abandoned, which is a continuation of application Ser. No. 875,212 filed Apr. 28,1992 (now abandoned) which itself is a continuation of application Ser. No. 530,457 filed May 30, 1990 (now abandoned), which is a continuation of Ser. No. 07/134,376, filed Dec. 17, 1987 (abandoned) which is a division of Ser. No. 06/767,332 filed Aug. 29, 1985 (now abandoned).
US Referenced Citations (2)
Number |
Name |
Date |
Kind |
4722840 |
Valenzu et al. |
Feb 1988 |
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5316931 |
Donson et al. |
May 1994 |
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Foreign Referenced Citations (1)
Number |
Date |
Country |
6553 |
Dec 1982 |
EPX |
Non-Patent Literature Citations (5)
Entry |
Maramorosch et al, Methods in Virology, vol. VII, Academic Press, N.Y. N.Y., 1984. pp. 265-266. |
Collmer et al. Virology 126:449-458, 198. |
Valenzuela et al (1982) Nature 298:347-50. |
Boujes-Bocquet et al (1984) Md. Approaches Vaccine, ed. Chonoch et al Cold Spring Harbor, Abst. |
Emini et al (1984) Virology 137:74-85. |
Divisions (2)
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Number |
Date |
Country |
Parent |
187135 |
Jan 1994 |
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Parent |
767332 |
Aug 1985 |
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Continuations (3)
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Number |
Date |
Country |
Parent |
875212 |
Apr 1992 |
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Parent |
530457 |
May 1990 |
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Parent |
134376 |
Dec 1987 |
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