Mutations in the BRCA1 gene

Abstract

Mutations resulting in stop codons in the BRCA1 gene are described. All of these mutations result in the formation of a truncated BRCA1 protein. Methods for identifying a sequence variation in a BRCA1 polynucleotide sequence are disclosed. The identification process includes allele specific sequence-based assays of known sequence variations. The methods can be used for efficient, and accurate detection of a mutation in a test BRCA1 gene sample for diagnostic and therapeutic purposes.

Description

FIELD OF THE INVENTION

[0001] This invention relates to the cancer suppressor gene BRCA1. More specifically, this invention detects germline mutations of the BRCA1 gene that are associated with breast and ovarian cancer, and somatic cell mutations of the BRCA1 gene indicating the nature of the cancer. Methods and reagents for detecting the presence of these mutations are included.

COPYRIGHT NOTICE

[0002] A portion of the disclosure of this patent document contains material which is subject to copyright protection. The copyright owner has no objection to the facsimile reproduction by anyone of the patent document or the patent disclosure as it appears in the U.S. Patent and Trademark Office patent file or records, but otherwise reserves all copyright rights whatsoever.

BACKGROUND OF THE INVENTION

[0003] BRCA1 is a putative tumor suppressor gene located on chromosome 17. Mutations in the BRCA1 gene are thought to account for roughly 45% of inherited breast cancer and 80-90% of families with increased risk of early onset breast and ovarian cancer (Easton, 1993, et al., American Journal of Human Genetics 52; 678-701). A compilation of the known BRCA1 mutations may be found at the Breast Cancer Information Core world wide web site at http:/Hwww.nhgri.nih.gov/Intramural_research/Lab_transfer/Bic/(BIC) (Friend, S. et al., 1995, Nature Genetics 11: 238). The BRCA1 gene is approximately 100,000 base pairs of genomic DNA encoding the 1836 amino acid BRCA1 protein. The sequence is divided into 24 separate exons. Exons 1 and 4 are noncoding, in that they are not part of the final functional BRCA1 protein product. Each exon consists of 200-400 bp, except for exon 11 which contains about 3600 bp (Weber, B., Science & Medicine (1996). A consensus sequence for the coding region of the human BRCA1 gene, referred to herein as BRCA1 (omi1), was first disclosed in application Ser. No. 08/598,591 (as SEQ ID NO:1, therein), herein incorporated by reference.

[0004] Accuracy in detecting mutations in BRCA1 is extremely important, particularly in clinical settings. Direct end-to-end sequencing of the gene potentially provides the most reliable results, given that an accurate reference sequence is available. However, direct sequencing is a cumbersome technique. Detection of one of many known or unknown mutations is further complicated when the gene is large and/or has a complex structure. The human BRCA1 gene, for example, is approximately 100,000 base pairs long and contains 24 exons (Weber, B., Science and Medicine, Scientific American January-February 1996, 12-21). Furthermore, in order to be practical and available to the general population, mutation detection methods must be efficient enough to accommodate a large number of different samples.

[0005] A number of techniques that are more rapid but less comprehensive than direct sequencing have been developed for detecting nucleotide sequence variations. These techniques may be used to detect differences between normal and mutant nucleotide sequences. DNA sequence-based allelic discrimination methods include: (1) allele-specific hybridization techniques, which effect detection under high stringent hybridization conditions; (2) single strand conformation polymorphism analysis and heteroduplex analysis which exploit differences in secondary structure of nucleic acid molecules; (3) denaturing gradient gel electrophoresis and constant denaturing gel electrophoresis which detect different alleles based on differences in melting behavior of nucleic acid molecules; (4) restriction enzyme cleavage, which discriminates between alleles based on the presence of absence of corresponding restriction recognition sequences; and (5) chemical or nuclease cleavage which detect base mismatch loci. Other techniques, such as the protein truncation test (Hogervorst F. B., et al., Nat. Genet. June 1995; 10(2):208-212), detect changes in the protein transcripts. For a summary of such techniques, see Marajver & Petty, 1996, Clinics in Lab. Med. 16: 139-167, especially Table 5 at p. 152.

[0006] One limitation of all these techniques is that sequence variations are often of unknown clinical significance. Since the triplet code is degenerate, many genetic variants do not alter the amino acid sequence of the resultant protein. Even those sequence alterations that do result in amino acid changes may not have a significant impact on protein function. For example, some amino acid changes will substitute functionally similar amino acids (conservative substitutions) such as one small neutrally charged amino acid for another. Further, some regions of a protein molecule may not be important for protein function. In those regions of the molecule, even large changes the amino acid sequence may be possible. Thus, genetic variants as a whole are often of unknown clinical significance.

[0007] If an altered sequence in the coding region of a gene associated with a condition such as cancer is found however, it is important to determine the clinical significance of the variant sequence. Knowing the significance of the sequence variation can be an invaluable tool in determining an appropriate treatment or monitoring regimen. For example, if an individual carries a mutation that interferes with protein function, it may be possible to provide the individual with increased expression of the gene through gene transfer therapy. It has been demonstrated that the gene transfer of the BRCA1 coding sequence into human cancer cells inhibits their growth and reduces tumorigenesis in nude mice. The BRCA1 protein product appears to be a secreted tumor growth inhibitor, making BRCA1 an ideal gene for gene therapy studies. Transduction of only a moderate percentage of tumor cells apparently produces enough growth inhibitor to inhibit all tumor cells. Arteaga, C. L., and J. T. Holt Cancer Research 56: 1098-1103 (1996); Holt, J. T. et al., Nature Genetics 12: 298-302 (1996). The observation of Holt et al. that the BRCA1 growth inhibitor is a secreted protein, also suggests the possibility of tumor suppression by injecting the growth irhibitor into the area of the tumor.

[0008] Efforts have independently focused on increasing the efficiency of DNA sequence analyses and increasing the comprehensiveness of the sequence-based techniques. There remains a need, however, for a comprehensive method of detecting mutations in individual gene samples that is both accurate enough to provide a reliable diagnosis to an individual patient and efficient enough to be practical for application to the general population.

[0009] Until now, the art has relied upon the occasional occurrence of a previously unreported mutation to increase its base of information for mutation testing in a given gene sequence. To determine the presence or absence of a mutation in a gene from a patient sample, the vast majority of test samples would be subject to complete end-to-end sequencing of the gene. This method is time consuming, often taking six weeks to obtain a result, and is extremely expensive. To ascertain the clinical significance of a previously unidentified sequence variation, those in the art would frequently rely on epidemiological data derived from an analysis of families or populations carrying the newly identified mutation. Other methods of assessing the significance of a newly identified sequence variation would include cloning the altered gene and studying its function in vitro or in vivo or comparing the altered gene sequence with homologous genes in other organisms. Given the realities of many of these genetic diseases, such an analysis comes too late to be of use to the individual bearing the newly identified sequence variation.

[0010] There is need in the art, therefore, for improved methods to identify clinically significant mutations. Identification of mutations of the BRCA1 gene would allow better tumor cell analysis for more appropriate therapy, and more widespread diagnostic screening for hereditary cancers than is currently possible, and also permit identification of critical or biologically significant functional areas deduced from the mutational spectrum observed. While mutations occur throughout the BRCA1 gene, there is a need for an assay, test or means for detecting mutations with a high sample number (throughput), sensitivity, accuracy and cost effectiveness.

[0011] The present invention addresses these needs and more by providing mutations, molecules, and methods useful for the identification of both known and unknown mutations.

SUMMARY OF THE INVENTION

[0012] It is an object of the invention to provide a method for determining a predisposition or higher susceptibility to cancers in individuals by determining the DNA sequence of the BRCA1 genes in the DNA of a patient specimen and comparing it to a naturally occurring (wild type) BRCA1 gene sequence or any haplotype thereof resulting from polymorphisms to determine whether the sample contains a mutation.

[0013] It is a further object of the invention to provide a method of characterizing and classifying a tumor and determining an appropriate therapy dependant upon the type of BRCA1 mutation(s) present.

[0014] It is also an object of the present invention to provide a non-chromosomal mutant BRCA1 gene and expressed mutant protein for drug development, gene therapy and other uses to prevent or ameliorate the effects of or resulting from the mutant BRCA1 gene.

[0015] It is another object of the present invention to prepare oligonucleotides, or groups of these oligonucleotides, where the oligonucleotide will specifically hybridize to either the wild type BRCA1 gene or a mutant BRCA1 gene to distinguish between the two BRCA1 genes.

[0016] It is still another object of the present invention to assay for the presence of a BRCA1 gene encoding a truncated BRCA1 protein by testing for the presence of a truncated BRCA1 protein.

[0017] The present invention is based on the discovery of numerous mutations in the published BRCA1 DNA sequence that result in a truncation of the BRCA1 protein. Mutations in the BRCA1 gene that cause truncations are associated with increased susceptibility to, and developmental stage of, different cancers, particularly breast and ovarian cancer. A truncated protein is likely to be non-functional or at least have a different biological activity.

DETAILED DESCRIPTION OF THE INVENTION

[0018] The present invention stems, in part, from the realization that previously unidentified sequence variations as a whole are typically of unknown clinical significance. At this time, epidemiological studies are time-consuming and may be of little use for infrequently identified sequence variations; laboratory studies of protein function are similarly time-consuming and difficult to extrapolate to human disease. However, mutations resulting in a prematurely terminated protein have a high probability of having clinical significance. In part, this is due to the fact that a premature termination of a coding sequence may entirely delete important functional domains, such as zinc fingers, transmembrane domains, phosphorylation sites, glycosylation sites, etc. It is also due, in part, to the fact that the removal of a portion of a protein molecule can significantly disrupt a protein's structure and function, even where no obvious functional domain has been deleted. Thus, the larger the truncation, the greater the likelihood that an important part of the molecule has been removed. Regardless of the cause, a survey of known clinically significant sequence variations (mutations) of the BRCA1 gene reveals that a large percentage of identified mutations are premature truncation mutations of the BRCA1 protein product. Thus, identifying those sequence variations that are premature truncation-causing mutations offers enormous predictive value to the clinician.

[0019] The presence of tumor cells with a mutation that inactivates BRCA1 may be clinically significant in determining the tumor stage, likelihood of metastasis, appropriate therapy etc. BRCA1 mutations appear in advanced tumors, several advanced primary cancers and cell lines derived from highly aggressive cancers. Individuals with one of their alleles having a BRCA1 mutation in their germline are at increased susceptibility for cancer, particularly breast and ovarian cancer.

[0020] With DNA sequencing technology, genomic DNA has been extracted from samples of whole blood, a cell line or a tumor and the coding regions of the BRCA1 gene were amplified using the polymerase chain reaction (PCR). Each of the coding regions has been sequenced completely and is recited in GENBANK, Accession Number 159546.

[0021] A number of mutations have been described in the BRCA1 gene that cause stop codons to be formed, thereby encoding a truncated BRCA1 protein. A list of mutations published to date is in TABLE 1.

[0022] The nomenclature of the published literature is inconsistent. See, for example, Beaudet et al, Human Mutations, 2: 245-248 (1993), Antonarakis et al, Human Mutations, 4: 166 (1994), Cotton, Human Mutations, 8: 197-202 (1996), and Beutler et al., Human Mutations, 8: 203-206 (1996). Consequently, the following nomenclature is used to define the mutations and polymorphisms of the present invention. In defining the mutation, the number indicates the nucleotide number corresponding to the BRCA1 gene sequence where the mutation first occurs. For simplified identification purposes, the BRCA1 sequence of SEQ ID NO:1, of U.S. Pat. No. 5,654,155 (GENBANK Accession number 159546) is used. However, the invention is equally applicable to other BRCA1 sequences. Other BRCA1 sequences (haplotypes) that are polymorphisms or genetic variations of BRCA1 may be used, in which case, a corresponding mutation at the corresponding nucleotide number is present.

[0023] Haplotypes are distinguished based on the combination of nucleotides present at particular polymorphic loci. Polymorphic nucleotide sites 2201, 2430, 2731, 3232, 3667, 4427, and 4956 usually define a haplotype. Other polymorphic sites of a BRCA1 gene are at Exon 4 (not coding) 49, IVS8-57, 1186, 2196, and 3238. Less common polymorphisms are at 233G>A, 561-34C>T, exon 9-6delT, 710C>T, 1100A>G, 1985G>T, 2202G>A, 2687T>C, 2933A>G, 3263T>C, 4077T>C, 4145A>G, 4193G>A, 4209-141C>A, 4364A>G, 4932T>C, 5106-68G>A, 5106-92G>A, intronic exon17-G>A, 5232G>T, 5272+66A>G, intronic exon18+A>G, 5396+48 12bp insert, 5396+4712bp insert, intronic exon 22+T>C, 5651C>T, 5657G>A, and UGA+36C>G. See, Couch et al., Human Mutations, 8(1):8-18 (1996), where polymorphisms have been reported.

[0024] As the BRCA1 gene numbering does not include introns, when the mutation is located within an intron, a nucleotide in the coding sequence +or − a number of nucleotides is given. Insertion mutations are indicated by “ins” and deletion mutations are indicated by “del”. Letters after “ins” or “del” refer to the nucleotide(s) which were inserted or deleted. Alternatively, where several bases have been inserted or deleted, a number may follow “ins” or “del,” indicating the number of affected bases. When the mutation results in one nucleotide being substituted for another, the original nucleotide(s) is placed to the left of the nucleotide number and the nucleotide(s) corresponding to the substituting nucleotide is placed to the right of the nucleotide number. In some cases, the substitution is indicated by both the original and the substituted nucleotides following the nucleotide number, with an “>” between them. In such cases the original nucleotide is to the left of the “>” and the substituted nucleotide is to the right of the “>,” as for example, in the designation 5657G>A.

TABLE 1

[0025] 185delAG, 185insA, 188del11, 189insA, 189insTGTC, 192del2, 259insT, 351delA, 448insA, 448delAG, 525insA, 589delCT, 613insT, 633delC, 787insA, 788delA, 794delT, 795delT, 816delGT, 916delTT, 917delTT, 926ins11, 962del4, 1048delA, 1049delG, 1099delCA, 1100delAT, 1103insC, 1129delA, 1135insA, 1191delC, 1201del11, 1205delGA, 1206delA, 1220insC, 1240delC, 1294del40, 1323delG, 1374delG, 138del29, 1395delT, 1406insA, 1411insT, 1438delT, 1459insG, 1479delAG, 1499insA, 1505delG, 1506delA, 1509delA, 1511insC, 151insC, 1559insA, 1611delC, 1623del5, 1670delT, 1675delA, 1701del7, 1768delA, 1832delS, 1942del4, 1996ins4, 2000del4, 2012insT, 2071insA, 2072del4, 2072insG, 2080delA, 2080delA, 2080insA, 2138delA, 2140delC, 2187delA, 2190delA, 2198delCA, 2229delAA, 2274insA, 2294delG, 2295delC, 2307insG, 2312del5, 2313del5, 2314del5, 231delAA, 2325delG, 2329delC, 2329delCA, 2334insCT, 2388delG, 2401delAA, 2415delAG, 2448delT, 2473insA, 2509delAA, 2569delG, 2575delC, 2576delC, 2594delC, 2594insA, 2595delA, 2596delC, 2711delA, 2731insT, 2765delTGC, 2795del4, 2798del4, 2800delAA, 2804delAA, 2809insA, 2819delTT, 2844del4, 2846delTCAA, 2862delTC, 2867insA, 2883del4, 2911delTGGT, 2925del4, 2953delGTA>insC, 2953insCdelGTA, 2954insT, 2982del5, 3039delTT, 3109insAA, 3121 delA, 3124delA, 3135del4, 3166ins5, 3172ins5, 3345delAG, 3345delAG, 3375insGA, 339insA, 3407delAA, 3411delCT, 3449insA, 3450del4, 3476delT, 3596del4, 3599dell, 3600del11, 3604delA, 3668delAGinsT, 3731delA, 3768insA, 3818delA, 3819del5, 3825del8, 3829delT, 3874del4, 3875del4, 3879insT, 3879insT, 3883insA, 3889delAG, 3890delGG, 3896delT, 3938insG, 3960delCA, 3977del4, 3988delAA, 4035delTT, 4050del4, 4091delG, 4154delA, 4184del4, 4239delAG, 4280delTC, 4284delAG, 4601delAA, 4693delAA, 5055delG, 5061delA, 5083del19, 5085del19, 5085del19, 5124delG, 5124delG, 5145del11, 5145del11, 5149del4, 5149del4, 5154del5, 5154del5, 5245delG, 5256delG, 5296del4, 5348delAA, 5382insC, 5389del7, 5404insG, 5438insC, 5439delAA, 5502insT, 5559delG, 5598insGA, 5629delG, 5640delA, 5677insA, A1518T, A2154T, C1599T, C1648G,. C1695T, C1740T, C1806T, C2257G, C2428A, C2457T, C297T, C3042T, C3508G, C3549T, C3726T, C3726T, C3726T, C3726T, C3837T, C3904A, C3960T, C3960T, C3960T, C4086T, C4302T, C4305T, C4341T, C4377T, C4446T, C4808G, C4929T, C5370T, C5622T, C624T, G1081A, G1177A, G1235A, G1371T, G1569T, G2307T, G2508T, G2841T, G3297T, G3759T, G3780T, G3867T, G4740T, G5199T, G5273A, G5292T, G5465A, G546T, G5563A, G5625T, G5630A, IVS12-1643del3835, IVS12-1643del3835, T1411G, T2035A, T3053G, T3358A, T3376G, T3458G, T5298A.

[0026] It should be noted that polymorphisms in the coding sequence are known and listed in TABE 2. An example is A180G. In the non-coding regions, a deleted T, 2 base pairs beyond exon 19 (5312+2delT) and a 12 base pair insertion 47 bases beyond exon 20 (5396+47ins12bp) are also known polymorphisms. Other genetic changes which may be polymorphisms or mutations are listed under mutations above. While the present invention references the sequences recited above, it is recognized that polymorphisms, either these recited or others, may be equally used for the purposes of the present invention.

TABLE 2

[0027] del exon 3, del561-702, del561-789, exon 18 delA, 5312+2delT, codong 1749 Pro to Arg, IVS20ins12, dup5396+48, A1100G, G1112C, C1120T, A1186G, A1186G, A120G, M11, D369del, T1256G, G1503A, T151C, A1546G, T1575C, C1605T, G1606A, G1630A, G1639T, T172C, C1735T, A1767C, A180G, 5544delGTT, C1822T, T1831C, T189C, G1985T, exon 9-2A>C, C2053A, G2093C, C212G, C2121T, G2196A, C2198T, C2201T, G2202A, A2286G, C2299T, G233A, G233A, A243G, T2430C, T2434C, G2531C, A2577G, C2596A, C2596A, C2640T, A2646G, T2687C, C2715T, C2731T, C2731T, C2731T, A2933G, T300G, C3030A, T309G, G310A, G310A, G3143A, A3165G, G3202A, A3232G, A3233G, G3238A, G3238A, T3263C, A330G, In6(+3)A>G, A3339G, C3415T, A3446G, T3529C, A3537G, G3543C, C3547T, C3567T, C3567T, A3667G, C3706A, G3720A, G3727A, G3776C, T378G, C3832T, G3867A, T388C, T4077C, A4145G, G4155A, A4158G, G4193A, 4209-141C>A, G4303A, A433G, G4364A, C4380T, C4427T, C4446G, Exon12+6,T>C, G4603T, G4654T, G4719A, G4755A, G5396+48ins12bp, C4801T, C480T, IVS7-3delT, C49T, A4931G, T4932C, A4935G, C4942T, A4956T, A4956G, A5001G, T5002C, T5002C, C5029T, G5040A, G5075A, G5076A, G5112A, G5112A, V1688del, V1688del, G5193A, G5193A, 5194-2A>C, C5214T, G5215A, C5232T, C5242A, C5242A, T5257C, G5263A, IVS18+1G>T, 5272+66A>G, I-18 5272+66G>A, 5272+66G>A, A5277G, 5280delCAG, A5317G, G5332A, A5335G, G5396A, 5396+1 G>A, G5396+1A, 5396+47ins12bp, T5443G, G546A, T5467C, G5482T, T5530A, C5535G, T5542C, T5548G, A5575G, G5586A, 561-34 C>T, G5616A, T5628C, C5651T, 5657G>A, A655G, C676A, G690A, C710T, C710T, G731C, T855G, G876A, G930A, G930C, exon 2-3insAG , IVS20+60ins12, Intron22T>C, T>G ins59bp, exon 9-6delT, G5396+47ins12bp, in18+1G>C, in20-1(G>T), IVS11-2delGT, IVS20ins12, IVS22+5G.A, IVS1-21insAT, IVS11-2A>G, IVS13+1G>T, IVS13+2T>G, IVS13-10C>T, IVS14-2A>G, IVS15+1G>A, IVS16+3G>C, IVS16+6T>C, IVS16-20A>G, IVS16-20A>G, IVS18+6T>G, IVS18-13A>G, IVS2+1G>A, IVS2+IG>T, IVS2-1 delT, IVS2-12C>G, IVS20+60ins12, IVS20-1G>A, IVS22+8T>C, IVS23-10C>A, IVS4-IG>T, IVS5+1G>T, IVS5-11T>G, IVS5-12A>G, IVS6+7G>A, IVS6-2A>T, IVS6-2delA, IVS7-3delT, IVS8+2T>A, IVS8-17G>T, IVS9+3G>A, exon24(+36)C>G, 3′UTR C>G (+36).

[0028] It should be noted that not all of the mutations listed in TABLES 1 and 2 are prior art as some of the mutations were published very recently after applicant determined his mutations.

[0029] Mutations detected according to the present invention are nonsense and frame shift mutations. Nonsense mutations cause an in-frame stop codon, which results in expression of a to truncated protein of presumably no BRCA1 functional ability or at least a significantly altered BRCA1 biological activity. Frameshift mutations cause an out-of-frame stop codon to be created in the inserted or deleted coding sequence. This formed stop codon may be at the site of mutation or downstream from the mutation. For the example of nonsense mutations, a nucleotide in a codon is mutated to provide a stop codon having a sequence of TAA, TAG or TGA. For the example of a frame shift mutation, 1, 2, 4, 5, or any larger integer not 3 or a multiple of three, nucleotides are inserted into, or deleted from, the BRCA1 gene sequence. Such mutations result in the formation of a stop codon at the codon containing the mutation or within codons downstream from the mutation site, but before the end of the wild type BRCA1 gene.

[0030] The presence of one or more of such mutations is expected to be clinically significant as they are capable of producing a truncating BRCA1 mutation. As the effects of truncations are predictably harmful, one has a high degree of certainty that the presence of such a mutation is clinically significant.

[0031] For example, truncating mutations 5382insC, 5438insC, and 185delAG are known to be associated with cancer (Abeliovich, et al., Am. J. Hum. Genet., 60:505-514 (1997); Struewing et al., Nat. Genetics, 11:198-200 (1995); Shattuck-Eidens, et al., JAMA 273(7):535-541 (1995). Accordingly, any truncating mutation deleting the same portion or more of the coding sequence is presumed to produce an affected mutant BRCA1 protein and to be associated with cancer or an increased susceptibility to cancer. 5382insC and 5438insC cause frame shifts, producing a stop codon (TGA) at nucleotides 5604-5606. 185delAG causes a frame shift, producing a stop codon (TGA) at nucleotides 234-236. Therefore, for the purposes of the present invention, truncating mutations at this location of a lower nucleotide number are presumed to be harmful or of clinical significance.

[0032] Conversely, the clinical significance of many missense mutations is unclear. Even when isolated from a tumor cell, the exact effect on BRCA1 protein must be independently shown. Theoretically, most variations in the BRCA1 sequence will be missense variations and relatively AD fewer will be truncating mutations. At the present time, the rules are unknown for which missense mutations in BRCA1 are predictably harmful or otherwise clinically significant. The BRCA1 gene is simply not sufficiently characterized, and given the history of mutations in other genes and computer programs attempting to find predictability, missense mutations will continue to be unpredictable. Without a certainty of their clinical significance, simply knowing that a missense variation exists may have no value.

[0033] In general, truncated proteins are non-functional or of reduced function by lacking proper biological activity. The presence of a BRCA1 gene encoding a truncated protein has a high probably of being clinically significant. This is in part due to important portions of the BRCA1 protein being located in the truncated region and not present in the expressed mutant protein. Important functional domains include, for example, any part of the active site, transmembrane domains, zinc fingers, phosphorylation sites, glycosylation sites, sites interacting with metal ions, coenzymes, cofactors, other ligands or receptors, etc. Furthermore, removal of part of the protein molecule or addition of amino acids to the sequence can significantly disrupt the secondary, tertiary or quaternary (if any) structure of the protein, even when no obvious functional domain has been deleted by the truncation.

[0034] For example, the 5382insC mutation is found in breast and ovarian cancers. This single base insertion creates a stop-codon at codon 1829, truncating the protein at a cysteine residue. This truncation deletes less than 2 percent of the coding sequence of BRCA1 . Accordingly, all truncations that delete this region of the BRCA1 gene would also by inference render the truncated BRCA1 protein non-functional for its putative tumor suppressor function. Mutant BRCA1 genes with even smaller portions of the gene being truncated are also known to be associated with cancer. For example, truncating mutations causing a stop codon at nucleotides 5676-5678, truncating 0.5% of the coding sequence, are also known to be associated with breast cancer (5677insA; Shattuck-Eidens et al., JAMA, 273(7):535-541 (1995)). Please note that several mutations in the DNA result in the expression of the same mutant BRCA1 protein because each mutation causes a stop codon to be formed at nucleotide site 5676-5678. This corresponds to codon 1853 (TAC—Tyrosine). Thus, all of these mutations are very likely to cause the same effective result, for example, in terms of susceptibility to cancer or cancer typing. Therefore, detecting these mutations would be diagnostically significant and useful for assessing the susceptibility for cancer. Given these mutations, oligonucleotides complementary to the F regions of these mutations are prepared to assay for the presence of these mutations in a sample.

[0035] For the present invention, the truncating mutations are detected in the BRCA1 gene or a fragment thereof which contains a mutation which directly or indirectly causes the formation of an in-frame stop codon (TAA, TAG or TGA) prematurely at any of codons 2 to 1863 A premature in-frame stop codon is one that occurs before the natural stop codon, which does not occur in a wild-type BRCA1 gene and when expressed results in a truncated protein product.

[0036] These BRCA1 mutations of the present invention may also be defined as specifically hybridizing to an oligonucleotide probe having the sequence 5′ R1-R2-R3 3′ wherein R1 is an oligonucleotide of at least three nucleotides, R2 is complementary to TAA, TAG or TGA, and R3 is an oligonucleotide of at least three nucleotides, the probe hybridizing to a premature in-frame stop codon on the mutant BRCA1 gene.

[0037] Whenever defining an oligonucleotide probe in the present invention, probes capable of potentially hybridizing to the sense strand are referred to. It should be recognized that oligonucleotide probes having a sequence complementary to these oligonucleotide probes may also be used and may specifically hybridize to the anti-sense strand. For diagnostic purposes either may be used, as DNA from biological samples is generally double stranded and contains both the sense and anti-sense strands.

[0038] In the present invention, the following BRCA1 mutations of the present invention represent one base changes that result in the formation of an in frame TAA, TAG or TGA. These mutations of the present invention are defined by forming stop codons at specific locations in accordance with TABLE 3. Any expressed protein from BRCA1 genes with these types of mutations should be truncated accordingly. The substituted nucleotide is indicated in lower case letters.

TABLE 3
List of Nonsense Mutations
	Stop Codon Formed	Nucleotide Number	Base Change
	TaA	127	T>A
	TgA	127	T>g
	tAA	144	G>T
	tAA	147	G>T
	tAA	153	C>T
	tAG	174	C>T
	tAA	177	A>T
	TaA	184	T>A
	TgA	184	T>g
	tAG	186	G>T
	TGa	191	T>A
	TGa	200	T>A
	tAG	204	G>T
	TaG	208	T>A
	tAG	213	A>T
	tAA	216	G>T
	tAG	231	A>T
	TGa	236	T>A
	TGa	251	C>A
	tAA	252	A>T
	TGa	260	C>A
	tAA	267	A>T
	tAG	279	C>T
	tAG	282	A>T
	tAA	285	A>T
	TaA	295	C>A
	TgA	295	C>g
	tAG	297	C>T
	TGa	302	T>A
	TaA	307	T>A
	TgA	307	T>g
	TGa	311	T>A
	tAG	312	A>T
	tAA	327	A>T
	tAA	339	C>T
	tAA	342	G>T
	tGA	351	A>T
	tAA	360	C>T
	tAA	369	G>T
	tAG	372	G>T
	TaG	379	T>A
	tAA	381	A>T
	TGa	392	T>A
	tAG	399	C>T
	TaG	415	T>A
	tAG	417	G>T
	TAa	422	T>A
	TAg	422	T>G
	TAa	434	T>A
	TAg	434	T>G
	tAA	444	A>T
	tAG	447	A>T
	tAA	450	G>T
	tAA	465	G>T
	tAA	474	A>T
	tAA	480	G>T
	tAA	495	C>T
	TAa	509	C>A
	TAg	509	C>G
	tGA	510	A>T
	tAA	522	A>T
	tGA	525	A>T
	tAG	534	C>T
	tAA	540	G>T
	tAA	546	G>T
	TaG	559	T>A
	tAG	561	C>T
	tAA	564	G>T
	tAA	582	C>T
	tGA	597	G>T
	tGA	606	A>T
	tAG	621	A>T
	tAG	624	C>T
	tAA	633	C>T
	tAA	639	C>T
	tAG	642	A>T
	TAa	656	C>A
	TAg	656	C>G
	tAA	660	G>T
	TaG	664	T>A
	tGA	666	G>T
	tAA	681	G>T
	tAG	696	A>T
	TAa	707	T>A
	TAg	707	T>G
	TGa	710	C>A
	tGA	717	G>T
	tAA	723	C>T
	tAA	726	G>T
	TaG	730	T>A
	TaA	733	T>A
	TgA	733	T>g
	tAA	735	C>T
	tAA	747	C>T
	tGA	750	G>T
	tAA	762	G>T
	TaG	772	T>A
	tAA	783	A>T
	tAG	786	A>T
	TGa	797	T>A
	tAA	798	G>T
	tAG	807	G>T
	tAA	828	G>T
	tAA	837	C>T
	TaG	856	T>A
	tAG	867	G>T
	tAG	870	A>T
	tAG	882	G>T
	tAA	894	G>T
	tAG	897	A>T
	TAa	902	T>A
	TAg	902	T>G
	tAG	903	C>T
	TaA	919	C>A
	TgA	919	C>g
	TaG	925	T>A
	tAG	933	G>T
	TGa	941	T>A
	TaA	964	C>A
	TgA	964	C>g
	TaA	967	T>A
	TgA	967	T>g
	tAG	969	C>T
	tAG	975	G>T
	TaA	988	T>A
	TgA	988	T>g
	TaA	991	T>A
	TgA	991	T>g
	tAA	999	A>T
	tGA	1005	A>T
	tAA	1017	G>T
	tAG	1020	A>T
	tAA	1026	G>T
	TGa	1034	T>A
	tAA	1038	A>T
	tAA	1044	A>T
	tAG	1047	C>T
	TaA	1057	T>A
	TgA	1057	T>g
	tAA	1068	C>T
	tGA	1077	A>T
	TaG	1081	G>A
	TGa	1082	G>A
	tGA	1086	G>T
	tAG	1092	A>T
	tAA	1095	G>T
	TGa	1103	T>A
	tAA	1128	G>T
	tAA	1131	A>T
	tAG	1134	A>T
	TGa	1163	T>A
	tAG	1164	G>T
	tGA	1167	A>T
	tAA	1170	A>T
	tAA	1173	G>T
	TaG	1177	G>A
	TGa	1178	G>A
	tAG	1182	A>T
	tAG	1185	C>T
	tAA	1188	A>T
	TGa	1199	C>A
	TaA	1201	C>A
	TgA	1201	C>g
	tAG	1203	G>T
	tGA	1212	A>T
	tAA	1221	G>T
	TaG	1234	G>A
	TGa	1235	G>A
	tAG	1257	C>T
	tAA	1260	A>T
	tAG	1269	G>T
	TaG	1273	G>A
	TGa	1274	G>A
	tGA	1281	A>T
	tAA	1290	G>T
	TaA	1297	T>A
	TgA	1297	T>g
	TaA	1312	C>A
	TgA	1312	C>g
	tAG	1323	G>T
	tAA	1329	G>T
	TaA	1333	C>A
	TgA	1333	C>g
	tAA	1341	A>T
	TaG	1357	T>A
	tAG	1371	G>T
	tAA	1380	G>T
	TAa	1385	T>A
	TAg	1385	T>G
	TaA	1396	C>A
	TgA	1396	C>g
	tAG	1398	G>T
	tAA	1401	A>T
	TaA	1411	T>A
	TgA	1411	T>g
	tAG	1431	G>T
	TaA	1438	T>A
	TgA	1438	T>g
	TGa	1445	T>A
	tAA	1446	A>T
	tAA	1452	G>T
	tGA	1455	A>T
	tAA	1467	A>T
	TaA	1471	C>A
	TgA	1471	C>g
	tAG	1476	G>T
	tAA	1488	G>T
	tAA	1494	A>T
	tAA	1506	A>T
	TAa	1514	T>A
	TAg	1514	T>G
	tAG	1518	A>T
	tAG	1521	A>T
	TaA	1540	T>A
	TgA	1540	T>g
	tAA	1554	G>T
	tGA	1569	G>T
	tAG	1584	G>T
	tAG	1590	C>T
	tAA	1599	C>T
	tAG	1602	G>T
	tAA	1620	A>T
	TaA	1624	T>A
	TgA	1624	T>g
	tAG	1626	A>T
	tAA	1632	A>T
	tGA	1638	A>T
	TaA	1648	C>A
	TgA	1648	C>g
	tAG	1662	G>T
	tAG	1674	A>T
	tAA	1677	A>T
	TaG	1687	T>A
	tAA	1695	C>T
	tAG	1698	A>T
	tAA	1707	G>T
	tAG	1719	C>T
	tGA	1722	G>T
	tAA	1731	C>T
	tAG	1737	G>T
	tAG	1740	C>T
	tAA	1749	C>T
	tAG	1779	G>T
	tAA	1785	A>T
	tAA	1791	A>T
	tAG	1806	C>T
	tAG	1812	G>T
	tAA	1815	A>T
	tAA	1833	G>T
	TaA	1837	C>A
	TgA	1837	C>g
	tAA	1842	G>T
	tAA	1845	A>T
	tAA	1848	G>T
	tAA	1860	A>T
	tAA	1866	A>T
	tAA	1872	G>T
	tAA	1902	G>T
	tAA	1908	G>T
	TaA	1912	T>A
	TgA	1912	T>g
	TaA	1927	C>A
	TgA	1927	C>g
	tAA	1929	A>T
	tAA	1938	A>T
	tAG	1941	A>T
	tAG	1959	A>T
	tAA	1989	G>T
	tGA	2004	A>T
	TGa	2027	T>A
	tAA	2031	G>T
	TaG	2035	T>A
	tAA	2037	C>T
	TGa	2051	T>A
	tAA	2061	G>T
	tAG	2064	G>T
	tAG	2070	A>T
	tAA	2073	A>T
	tAA	2076	A>T
	tAG	2079	A>T
	TAa	2084	C>A
	TAg	2084	C>G
	tAA	2088	C>T
	tGA	2109	A>T
	tAA	2118	C>T
	tAA	2127	G>T
	tAA	2133	A>T
	tAA	2136	G>T
	tGA	2148	G>T
	tAG	2154	A>T
	tAG	2157	A>T
	tAG	2166	A>T
	tAA	2175	G>T
	tAG	2178	C>T
	tAA	2187	A>T
	tGA	2190	A>T
	tAG	2214	G>T
	tAG	2220	A>T
	TaA	2224	T>A
	TgA	2224	T>g
	tAG	2250	A>T
	TGa	2255	T>A
	TaA	2257	C>A
	TgA	2257	C>g
	tAA	2268	G>T
	tAA	2274	A>T
	tAA	2277	G>T
	tGA	2301	A>T
	tAA	2304	G>T
	tAA	2307	G>T
	tAA	2310	A>T
	tAA	2313	G>T
	tAG	2316	G>T
	tAA	2319	A>T
	tAA	2325	G>T
	tAA	2334	A>T
	tAA	2352	G>T
	tAA	2361	A>T
	TaA	2374	T>A
	TgA	2374	T>g
	tGA	2379	G>T
	tAA	2382	G>T
	TaG	2392	T>A
	tAA	2394	C>T
	tAA	2400	G>T
	tGA	2403	A>T
	tAG	2412	G>T
	TaA	2428	C>A
	TgA	2428	C>g
	TAa	2450	T>A
	TAg	2450	T>G
	tAG	2457	C>T
	tAA	2460	G>T
	TaG	2470	C>A
	TaA	2473	T>A
	TgA	2473	T>g
	tAA	2478	G>T
	tAG	2496	A>T
	tAA	2502	A>T
	tAA	2508	G>T
	tAA	2517	A>T
	TGa	2522	T>A
	tAG	2529	C>T
	TGa	2534	T>A
	tAA	2544	G>T
	tAG	2553	A>T
	tGA	2556	G>T
	TGa	2573	T>A
	tAA	2577	A>T
	tGA	2586	A>T
	tAA	2598	G>T
	tAG	2607	A>T
	TAa	2612	T>A
	TAg	2612	T>G
	TaG	2617	T>A
	tGA	2619	G>T
	tAA	2625	G>T
	tAA	2643	G>T
	tAA	2655	G>T
	tAA	2661	G>T
	tAA	2664	G>T
	tAA	2670	G>T
	tAG	2682	C>T
	TAa	2687	T>A
	TAg	2687	T>G
	TaG	2689	T>A
	tAG	2691	C>T
	tAG	2703	A>T
	TaA	2710	C>A
	TgA	2710	C>g
	tAG	2712	A>T
	tAG	2718	C>T
	TaA	2722	C>A
	TgA	2722	C>g
	TaA	2737	C>A
	TgA	2737	C>g
	tGA	2745	G>T
	tAA	2754	G>T
	tAG	2757	G>T
	tAA	2760	G>T
	TGa	2765	T>A
	TaA	2794	T>A
	TgA	2794	T>g
	tAG	2796	A>T
	tAA	2799	A>T
	tAA	2802	C>T
	tAA	2811	A>T
	tAA	2823	G>T
	TGa	2828	T>A
	tAA	2829	G>T
	tAA	2832	C>T
	tAG	2835	A>T
	tAA	2838	G>T
	tAA	2841	G>T
	tAA	2847	C>T
	tGA	2850	G>T
	tAG	2853	A>T
	tAG	2859	G>T
	tAG	2871	A>T
	tAG	2880	C>T
	tAG	2919	C>T
	tAA	2922	A>T
	tAG	2928	A>T
	tAA	2946	A>T
	TGa	2951	T>A
	tAA	2958	A>T
	tGA	2961	G>T
	TGa	2978	T>A
	TaA	2983	C>A
	TgA	2983	C>g
	tAG	2988	C>T
	tGA	2994	A>T
	tAA	3003	G>T
	tGA	3009	G>T
	tAA	3027	A>T
	tGA	3033	G>T
	TaA	3040	T>A
	TgA	3040	T>g
	tAA	3042	C>T
	TAa	3053	T>A
	TAg	3053	T>G
	tAG	3078	A>T
	TaA	3082	C>A
	TgA	3082	C>g
	tAA	3090	A>T
	tAA	3096	A>T
	TGa	3101	T>A
	tAG	3102	A>T
	tAA	3105	A>T
	tAG	3117	G>T
	tAA	3120	G>T
	tAG	3129	G>T
	tAA	3132	G>T
	TaA	3139	C>A
	TgA	3139	C>g
	TaA	3145	C>A
	TgA	3145	C>g
	tAA	3150	G>T
	tGA	3153	A>T
	tAA	3156	G>T
	tGA	3162	G>T
	tAG	3168	G>T
	tGA	3213	A>T
	tAA	3216	G>T
	tAA	3228	A>T
	tGA	3231	G>T
	TaA	3241	C>A
	TgA	3241	C>g
	tAA	3255	G>T
	tAA	3276	G>T
	tAA	3297	G>T
	tAA	3315	G>T
	tAA	3324	C>T
	tAA	3330	G>T
	tGA	3339	A>T
	tGA	3345	A>T
	tAA	3354	A>T
	TaG	3358	T>A
	tGA	3372	A>T
	TaA	3376	T>A
	TgA	3376	T>g
	TaG	3385	T>A
	tAA	3387	C>T
	tAG	3393	G>T
	TAa	3401	T>A
	TAg	3401	T>G
	tAA	3402	A>T
	tAA	3405	C>T
	tGA	3417	G>T
	TGa	3428	T>A
	tAG	3429	A>T
	tAA	3438	G>T
	tAA	3444	A>T
	tAG	3447	A>T
	tAA	3450	C>T
	tAA	3453	G>T
	TAa	3458	T>A
	TAg	3458	T>G
	tAA	3459	G>T
	tAA	3462	G>T
	tAG	3471	C>T
	TAa	3500	T>A
	TAg	3500	T>G
	TaA	3508	C>A
	TgA	3508	C>g
	TaA	3517	T>A
	TgA	3517	T>g
	tAA	3519	G>T
	tAG	3522	C>T
	tGA	3531	G>T
	tAG	3549	C>T
	TGa	3557	T>A
	tAG	3561	G>T
	TaA	3580	T>A
	TgA	3580	T>g
	tAA	3591	G>T
	tAG	3597	A>T
	tAA	3600	G>T
	tAA	3618	G>T
	tAG	3630	A>T
	tAA	3633	G>T
	tAA	3654	A>T
	tAG	3663	C>T
	tGA	3666	A>T
	tGA	3669	G>T
	tAG	3672	G>T
	TaG	3712	T>A
	tAG	3717	C>T
	TAa	3725	C>A
	TAg	3725	C>G
	tGA	3726	C>T
	tGA	3729	A>T
	tAG	3738	A>T
	tAA	3741	A>T
	TaA	3745	T>A
	TgA	3745	T>g
	tAG	3747	G>T
	TaA	3754	C>A
	TgA	3754	C>g
	tAA	3756	G>T
	tAG	3759	G>T
	TaA	3766	T>A
	TgA	3766	T>g
	tAG	3774	G>T
	tAA	3780	G>T
	tAG	3783	G>T
	TGa	3794	C>A
	tAA	3798	C>T
	TaG	3805	T>A
	TaA	3808	T>A
	TgA	3808	T>g
	tAA	3816	A>T
	tAG	3837	C>T
	tAG	3867	G>T
	TGa	3872	T>A
	tAG	3879	A>T
	tAG	3888	G>T
	tAG	3891	G>T
	TaA	3898	T>A
	TgA	3898	T>g
	TaA	3901	T>A
	TgA	3901	T>g
	TaA	3904	C>A
	TgA	3904	C>g
	TaG	3907	T>A
	tAG	3909	A>T
	TaA	3919	T>A
	TgA	3919	T>g
	TGa	3929	C>A
	tAG	3936	C>T
	TaG	3946	T>A
	tAG	3951	A>T
	tAG	3960	C>T
	tAA	3963	G>T
	tAG	3978	G>T
	tAA	3981	G>T
	tAA	3987	A>T
	TGa	3992	T>A
	TaG	4003	T>A
	TaA	4012	C>A
	TgA	4012	C>g
	tAG	4014	C>T
	TGa	4019	C>A
	tAA	4023	G>T
	TaG	4027	T>A
	tAA	4029	G>T
	TaG	4036	T>A
	tAG	4056	C>T
	TaG	4069	T>A
	tAA	4083	A>T
	tAA	4086	C>T
	tAG	4098	C>T
	tAA	4104	G>T
	tAG	4110	C>T
	tGA	4113	G>T
	tAG	4131	A>T
	tAA	4134	G>T
	TaG	4138	T>A
	TaA	4144	C>A
	TgA	4144	C>g
	tAA	4152	G>T
	tAA	4155	G>T
	tGA	4158	A>T
	tGA	4161	G>T
	TaG	4171	T>A
	tAA	4173	G>T
	tAA	4176	G>T
	tAA	4185	C>T
	tAA	4188	G>T
	tAG	4191	G>T
	tAA	4194	C>T
	TaA	4207	C>A
	TgA	4207	C>g
	TaA	4213	T>A
	TgA	4213	T>g
	tAA	4218	G>T
	TGa	4235	T>A
	tAG	4236	G>T
	tAA	4242	G>T
	tAA	4257	G>T
	TGa	4265	C>A
	TaA	4267	C>A
	TgA	4267	C>g
	tAG	4281	C>T
	TaA	4294	T>A
	TgA	4294	T>g
	tAG	4302	C>T
	tAG	4305	C>T
	tAA	4320	C>T
	tAG	4335	A>T
	tAG	4341	C>T
	tAG	4344	C>T
	tAA	4347	G>T
	tAA	4356	G>T
	tAA	4362	G>T
	TaA	4372	T>A
	TgA	4372	T>g
	tAA	4374	G>T
	tAG	4377	C>T
	tAG	4389	C>T
	TAa	4406	C>A
	TAg	4406	C>G
	tAG	4437	G>T
	tGA	4446	C>T
	tAA	4455	G>T
	tAA	4458	C>T
	TaA	4468	C>A
	TgA	4468	C>g
	tAA	4470	G>T
	tAA	4473	A>T
	TaA	4483	T>A
	TgA	4483	T>g
	TaA	4489	C>A
	TgA	4489	C>g
	tAG	4491	C>T
	tAA	4494	A>T
	tAA	4503	G>T
	TAa	4508	C>A
	TAg	4508	C>G
	tAG	4518	C>T
	tAA	4527	G>T
	tAG	4545	A>T
	tAG	4551	G>T
	tAA	4578	A>T
	tAA	4584	A>T
	tAA	4587	G>T
	tGA	4593	G>T
	tAA	4599	G>T
	TaA	4606	C>A
	TgA	4606	C>g
	tAA	4617	A>T
	TGa	4622	C>A
	TaA	4627	C>A
	TgA	4627	C>g
	TaA	4630	T>A
	TgA	4630	T>g
	TaG	4642	G>A
	TGa	4643	G>A
	TAa	4646	C>A
	TAg	4646	C>G
	TGa	4658	C>A
	tAG	4671	C>T
	tGA	4677	A>T
	TAa	4685	C>A
	TAg	4685	C>G
	tAA	4692	C>T
	tAG	4695	G>T
	tAG	4698	G>T
	tAG	4707	A>T
	tAG	4722	G>T
	tAG	4725	G>T
	tAA	4728	C>T
	tAG	4731	C>T
	tAA	4737	G>T
	tAG	4740	G>T
	TaG	4759	T>A
	tAA	4764	G>T
	TAa	4775	C>A
	TAg	4775	C>G
	TaG	4777	T>A
	tAA	4785	C>T
	tAG	4794	G>T
	tGA	4797	G>T
	TAa	4808	C>A
	TAg	4808	C>G
	tAA	4812	G>T
	tGA	4818	G>T
	tAA	4845	G>T
	tAA	4860	G>T
	tGA	4866	A>T
	tAG	4875	G>T
	TaA	4879	C>A
	TgA	4879	C>g
	TaA	4906	C>A
	TgA	4906	C>g
	TaG	4918	T>A
	tAA	4920	A>T
	tAA	4929	C>T
	TaG	4933	T>A
	tAA	4935	A>T
	tAA	4944	G>T
	tAG	4953	C>T
	TAa	4994	T>A
	TAg	4994	T>G
	tAA	5004	G>T
	tAA	5007	G>T
	tAG	5022	G>T
	tAG	5025	A>T
	tAA	5031	G>T
	TaG	5035	T>A
	TaA	5044	C>A
	TgA	5044	C>g
	tAA	5049	G>T
	tAA	5061	A>T
	tGA	5064	A>T
	tAA	5097	G>T
	tAA	5100	G>T
	TAa	5117	C>A
	TAg	5117	C>G
	tAG	5118	A>T
	tGA	5127	A>T
	tAA	5130	A>T
	TaA	5146	T>A
	TgA	5146	T>g
	tAA	5163	G>T
	tAG	5166	G>T
	tAA	5187	A>T
	tAG	5199	G>T
	TGa	5210	T>A
	tAA	5211	G>T
	tAA	5223	A>T
	TAa	5228	T>A
	TAg	5228	T>G
	tGA	5235	G>T
	tGA	5244	G>T
	tGA	5247	G>T
	tAA	5250	A>T
	TaG	5254	G>A
	TGa	5255	G>A
	TAa	5267	T>A
	TAg	5267	T>G
	TaG	5272	G>A
	TGa	5273	G>A
	tAG	5280	C>T
	tAA	5289	A>T
	tAA	5292	G>T
	tGA	5295	A>T
	tAA	5298	A>T
	tAG	5310	G>T
	tAA	5322	G>T
	tGA	5328	A>T
	tGA	5331	G>T
	tGA	5346	G>T
	tGA	5349	A>T
	tAA	5358	C>T
	tAG	5367	A>T
	tGA	5370	C>T
	tGA	5376	A>T
	tAA	5379	G>T
	tAG	5385	C>T
	tGA	5391	A>T
	tAG	5394	A>T
	tAA	5412	G>T
	TGa	5420	T>A
	TGa	5423	C>A
	TAa	5426	T>A
	TAg	5426	T>G
	tAA	5454	C>T
	tAA	5460	G>T
	TaG	5464	G>A
	TGa	5465	G>A
	tAG	5472	C>T
	TGa	5480	T>A
	tAG	5496	A>T
	tAG	5499	G>T
	TaA	5506	C>A
	TgA	5506	C>g
	TaA	5509	C>A
	TgA	5509	C>g
	tAG	5550	C>T
	TaG	5563	G>A
	TGa	5564	G>A
	tAG	5568	G>T
	tAG	5595	C>T
	TGa	5603	T>A
	tAG	5604	G>T
	tGA	5622	C>T
	tAG	5625	G>T
	TaG	5629	G>A
	TGa	5630	G>A
	TaG	5635	T>A
	TAa	5654	C>A
	TAg	5654	C>G
	tAG	5655	C>T
	TGa	5660	C>A
	tAG	5661	C>T
	tAG	5664	G>T
	TAa	5678	C>A
	TAg	5678	C>G
	tAG	5688	C>T
	TAa	5708	C>A
	TAg	5708	C>G
	TaA	5710	G>A

[0039] The genes of the present invention containing nonsense mutations are also defined as BRCA1 genes having the sequence 5′ R1-R2-R3 3′; where R1 is the wild type BRCA1 DNA sequence from codon 1 to X−1; R2 is TAA, TAG or TGA; R3 is the wild type BRCA1 DNA sequence from codon X+1 to 1862; and where X=2 to 1861.

[0040] The genes of the present invention containing nonsense mutations are also defined by being capable of specifically hybridizing to an oligonucleotide probe having at least 12 nucleotides in length and having the sequence 5′ R1-R2-R3 3′; where R1 contains at its 3′ end three nucleotides complementary to codon X−1 of the wild-type BRCA1 gene; R2=a sequence complementary to TAG, TGA or TAA; R3 contains at its 5′ end three nucleotides complementary to codon X+1 of the wild type BRCA1 gene; where X=2 to 1862 Other oligonucleotide probes complementary to these probes are also acceptable, hybridizing to the antisense strand. The oligonucleotide probe is unable to specifically hybridize to the wild-type BRCA1 gene with the same binding affinity as to the mutant BRCA1 gene.

[0041] The present invention also involves frame shift mutations involving insertions or deletions of 1, 2, 4, 5, 7, 8, or any other number which is not 3 or a multiple of 3, nucleotides. Single base deletions of the present invention form one or more stop codons as indicated in the following TABLE 4. The formed in-frame stop codon and the location are provided. Any expressed protein from BRCA1 genes with these types of mutations should be truncated accordingly. It should be recognized that the present invention includes deletions of 3n+1 bases, where n is an integer greater than zero and less than 1862. These larger deletions mutations have stop codons at nucleotide numbers corresponding to the listed stop codon at the nucleotide number listed. The corresponding nucleotide numbers of the stop codons will be 3n nucleotides smaller than those listed.

TABLE 4
Single Base Deletions
Codon Formed Nucleotide Number
TAG          183-185
TGA          207-209
TGA          264-266
TGA          249-251
TGA          258-260
TGA          309-311
TAA          321-323
TGA          378-380
TAA          471-473
TAG          420-422
TAA          432-434
TGA          603-605
TAA          507-509
TGA          612-614
TAA          816-818
TAA          654-656
TGA          708-710
TGA          855-857
TGA          1008-1010
TAA          918-920
TAG          1014-1016
TAG          1056-1058
TGA          1032-1034
TAG          1137-1139
TGA          1101-1103
TGA          1143-1145
TAA          1236-1238
TGA          1176-1178
TAG          1200-1202
TGA          1233-1235
TAA          1242-1244
TAG          1296-1298
TAG          1344-1346
TAA          1332-1334
TAA          1365-1367
TAG          1374-1376
TAG          1404-1406
TAG          1395-1397
TAA          1437-1439
TAG          1473-1475
TGA          1443-1445
TAG          1470-1472
TAA          1539-1541
TAA          1548-1550
TAA          1560-1562
TAG          1566-1568
TAA          1593-1595
TAA          1623-1625
TGA          1710-1712
TAG          1647-1649
TAA          1713-1715
TGA          1752-1754
TGA          1755-1757
TAG          1830-1832
TAA          1878-1880
TAA          1890-1892
TAA          1911-1913
TGA          1950-1952
TAA          1926-1928
TAG          1992-1994
TAG          1995-1997
TAA          2010-2012
TAA          2067-2069
TGA          2025-2027
TAA          2067-2069
TGA          2217-2219
TAA          2082-2084
TGA          2217-2219
TAA          2223-2225
TAG          2322-2324
TAA          2256-2258
TAG          2322-2324
TAA          2373-2375
TAG          2409-2411
TAG          2490-2492
TAG          2448-2450
TAG          2490-2492
TGA          2523-2525
TAA          2559-2561
TAG          2652-2654
TAA          2793-2795
TAA          2709-2711
TAA          2793-2795
TAA          2736-2738
TAA          2793-2795
TAG          3114-3116
TGA          2949-2951
TAG          3114-3116
TGA          3099-3101
TAG          3114-3116
TGA          3186-3188
TAA          3138-3140
TGA          3186-3188
TAG          3258-3260
TAA          3240-3242
TAG          3258-3260
TAG          3300-3302
TAG          3333-3335
TGA          3357-3359
TAG          3375-3377
TAA          3441-3443
TAA          3399-3401
TAA          3441-3443
TGA          3426-3428
TAA          3441-3443
TAG          3465-3467
TAG          3456-3458
TAG          3465-3467
TGA          3501-3503
TAG          3516-3518
TAG          3507-3509
TAG          3516-3518
TAG          3579-3581
TAA          3594-3596
TAG          3744-3746
TAA          3819-3821
TAG          3753-3755
TAA          3819-3821
TGA          3906-3908
TAA          3918-3920
TAA          3939-3941
TGA          3927-3929
TAA          3939-3941
TGA          4035-4037
TGA          4017-4019
TGA          4035-4037
TGA          4068-4070
TGA          4089-4091
TGA          4122-4124
TAG          4212-4214
TAG          4143-4145
TAG          4212-4214
TAA          4206-4208
TAG          4212-4214
TAA          4293-4295
TAG          4266-4268
TAA          4293-4295
TGA          4329-4331
TAA          4332-4334
TAG          4359-4361
TAG          4371-4373
TAA          4416-4418
TAA          4482-4484
TAG          4467-4469
TAA          4482-4484
TAA          4512-4514
TAG          4629-4631
TGA          4758-4760
TAA          4644-4646
TGA          4758-4760
TAG          4791-4793
TGA          4917-4919
TAG          4878-4880
TGA          4917-4919
TAA          4905-4907
TGA          4917-4919
TGA          4932-4934
TGA          5013-5015
TAA          4992-4994
TGA          5013-5015
TGA          5034-5036
TGA          5088-5090
TAA          5043-5045
TGA          5088-5090
TAA          5145-5147
TAA          5115-5117
TAA          5145-5147
TAA          5154-5156
TGA          5184-5186
TGA          5220-5222
TAG          5232-5234
TAG          5256-5258
TGA          5274-5276
TGA          5304-5306
TAG          5409-5411
TGA          5493-5495
TAG          5424-5426
TGA          5463-5465
TGA          5616-5618
TGA          5562-5564
TGA          5616-5618
TAG          5643-5645
TGA          5679-5681

[0042] Two base deletions of the present invention form stop codons as indicated in the following TABLE 5. The formed in-frame stop codon and its location are provided. Any expressed protein from BRCA1 genes with these types of mutations should be truncated accordingly. It should be recognized that the present invention includes deletions of 3n+2 bases, where n is an integer greater than zero and less than 1862. These larger deletions mutations have stop codons at nucleotide numbers corresponding to the listed stop codon at the nucleotide number listed. The corresponding nucleotide numbers of the stop codons will be 3n nucleotides smaller than those listed.

TABLE 5
Two Base Deletions
Codon Formed Nucleotide Number
TGA          141-143
TAA          162-164
TGA          234-236
TGA          183-185
TGA          234-236
TAA          309-311
TGA          249-251
TGA          309-311
TGA          315-317
TAG          354-356
TGA          366-368
TGA          402-404
TAA          378-380
TGA          402-404
TAA          432-434
TGA          414-416
TAA          432-434
TAA          453-455
TGA          462-464
TGA          477-479
TGA          537-539
TAG          507-509
TGA          537-539
TAA          588-590
TGA          657-659
TGA          669-671
TGA          678-680
TAA          690-692
TAA          693-695
TGA          759-761
TAG          705-707
TGA          759-761
TAG          708-710
TGA          759-761
TGA          795-797
TGA          771-773
TGA          795-797
TGA          804-806
TGA          825-827
TAA          843-845
TAA          846-848
TGA          849-851
TGA          864-866
TAA          855-857
TGA          864-866
TGA          879-881
TAG          906-908
TAA          900-902
TAG          906-908
TGA          972-974
TAA          918-920
TGA          972-974
TAA          996-998
TGA          1023-1025
TGA          1032-1034
TAA          1035-1037
TAA          1071-1073
TAA          1089-1091
TGA          1101-1103
TGA          1104-1106
TAG          1107-1109
TGA          1149-1151
TGA          1161-1163
TAA          1179-1181
TGA          1176-1178
TAA          1179-1181
TAG          1209-1211
TGA          1200-1202
TAG          1209-1211
TGA          1218-1220
TAG          1245-1247
TAA          1263-1265
TGA          1266-1268
TGA          1284-1286
TAG          1278-1280
TGA          1284-1286
TGA          1287-1289
TGA          1302-1304
TGA          1305-1307
TGA          1314-1316
TGA          1326-1328
TGA          1347-1349
TAA          1332-1334
TGA          1347-1349
TGA          1368-1370
TGA          1356-1358
TGA          1368-1370
TGA          1377-1379
TGA          1419-1421
TGA          1395-1397
TGA          1419-1421
TGA          1428-1430
TGA          1443-1445
TGA          1449-1451
TAA          1479-1481
TAA          1464-1466
TAA          1479-1481
TGA          1485-1487
TGA          1551-1553
TAG          1512-1514
TGA          1551-1553
TAG          1539-1541
TGA          1551-1553
TGA          1581-1583
TAA          1617-1619
TAA          1629-1631
TAA          1623-1625
TAA          1629-1631
TGA          1659-1661
TGA          1704-1706
TAA          1725-1727
TAA          1764-1766
TAG          1767-1769
TGA          1776-1778
TAA          1782-1784
TGA          1794-1796
TGA          1809-1811
TAA          1821-1823
TGA          1869-1871
TAA          1857-1859
TGA          1869-1871
TAA          1935-1937
TAA          1911-1913
TAA          1935-1937
TAA          1926-1928
TAA          1935-1937
TAG          1944-1946
TGA          1986-1988
TAG          2001-2003
TAA          2019-2021
TGA          2028-2030
TGA          2040-2042
TAG          2043-2045
TAG          2052-2054
TGA          2058-2060
TAA          2130-2132
TAA          2082-2084
TAA          2130-2132
TAA          2160-2162
TGA          2172-2174
TAA          2184-2186
TGA          2193-2195
TGA          2199-2201
TAA          2247-2249
TGA          2265-2267
TAA          2256-2258
TGA          2265-2267
TAA          2271-2273
TAG          2289-2291
TAA          2331-2333
TAA          2340-2342
TAA          2343-2345
TGA          2349-2351
TGA          2397-2399
TAG          2373-2375
TGA          2397-2399
TAG          2415-2417
TGA          2442-2444
TAG          2481-2483
TAG          2448-2450
TAG          2481-2483
TAA          2514-2516
TGA          2541-2543
TAA          2580-2582
TAA          2574-2576
TAA          2580-2582
TAG          2583-2585
TGA          2589-2591
TAA          2604-2606
TGA          2622-2624
TAA          2628-2630
TGA          2667-2669
TGA          2673-2675
TGA          2820-2822
TAA          2700-2702
TGA          2820-2822
TAA          2709-2711
TGA          2820-2822
TAA          2736-2738
TGA          2820-2822
TAA          2793-2795
TGA          2820-2822
TGA          2826-2828
TGA          2856-2858
TAA          2862-2864
TAA          2886-2888
TAA          2925-2927
TGA          2934-2936
TAA          2937-2939
TAG          2949-2951
TAG          2967-2969
TAA          3024-3026
TAG          2991-2993
TAA          3024-3026
TAA          3087-3089
TAG          3051-3053
TAA          3087-3089
TAA          3093-3095
TGA          3099-3101
TGA          3126-3128
TGA          3147-3149
TGA          3165-3167
TAG          3195-3197
TAA          3201-3203
TAA          3204-3206
TAG          3210-3212
TAA          3225-3227
TAA          3249-3251
TAG          3240-3242
TAA          3249-3251
TGA          3252-3254
TAA          3270-3272
TAG          3264-3266
TAA          3270-3272
TGA          3273-3275
TAA          3291-3293
TAG          3285-3287
TAA          3291-3293
TGA          3294-3296
TGA          3309-3311
TAG          3306-3308
TGA          3309-3311
TGA          3312-3314
TAG          3336-3338
TAG          3369-3371
TAA          3357-3359
TAG          3369-3371
TGA          3390-3392
TAA          3399-3401
TAA          3420-3422
TGA          3426-3428
TGA          3435-3437
TAA          3456-3458
TAA          3477-3479
TAA          3510-3512
TGA          3507-3509
TAA          3510-3512
TAG          3534-3536
TGA          3516-3518
TAG          3534-3536
TGA          3558-3560
TGA          3567-3569
TGA          3570-3572
TGA          3582-3584
TGA          3579-3581
TGA          3582-3584
TGA          3588-3590
TAG          3606-3608
TGA          3615-3617
TGA          3621-3623
TAA          3627-3629
TAG          3648-3650
TAG          3675-3677
TAG          3687-3689
TAG          3768-3770
TAG          3723-3725
TAG          3768-3770
TGA          3744-3746
TAG          3768-3770
TGA          3753-3755
TAG          3768-3770
TGA          3771-3773
TGA          3777-3779
TAA          3813-3815
TAG          3843-3845
TAG          3849-3851
TAA          3876-3878
TAG          3912-3914
TAA          3906-3908
TAG          3912-3914
TGA          3921-3923
TAA          3918-3920
TGA          3921-3923
TAA          3930-3932
TAG          3927-3929
TAA          3930-3932
TAG          3972-3974
TGA          3975-3977
TAG          3996-3998
TGA          4020-4022
TAG          4017-4019
TGA          4020-4022
TGA          4101-4103
TGA          4026-4028
TGA          4101-4103
TAA          4080-4082
TGA          4101-4103
TGA          4125-4127
TGA          4146-4148
TGA          4143-4145
TGA          4146-4148
TGA          4149-4151
TAA          4179-4181
TGA          4170-4172
TAA          4179-4181
TGA          4215-4217
TAA          4206-4208
TGA          4215-4217
TGA          4233-4235
TGA          4239-4241
TGA          4254-4256
TGA          4284-4286
TAA          4323-4325
TGA          4353-4355
TAA          4395-4397
TGA          4371-4373
TAA          4395-4397
TGA          4419-4421
TGA          4434-4436
TAG          4497-4499
TGA          4467-4469
TAG          4497-4499
TGA          4500-4502
TGA          4539-4541
TGA          4548-4550
TAG          4563-4565
TAA          4575-4577
TAA          4581-4583
TAA          4614-4616
TGA          4632-4634
TGA          4629-4631
TGA          4632-4634
TAG          4635-4637
TAG          4674-4676
TGA          4641-4643
TAG          4674-4676
TAA          4704-4706
TGA          4713-4715
TGA          4833-4835
TGA          4836-4838
TGA          4842-4844
TGA          4848-4850
TGA          4857-4859
TGA          4977-4979
TAA          4917-4919
TGA          4977-4979
TAA          4932-4934
TGA          4977-4979
TAA          4992-4994
TAA          5148-5150
TAA          5115-5117
TAA          5148-5150
TGA          5160-5162
TGA          5196-5198
TGA          5208-5210
TAG          5259-5261
TAA          5286-5288
TGA          5307-5309
TGA          5313-5315
TGA          5319-5321
TGA          5601-5603
TAG          5400-5402
TGA          5601-5603
TGA          5421-5423
TGA          5601-5603
TAG          5424-5426
TGA          5601-5603
TGA          5634-5636
TAA          5652-5654
TGA          5658-5660
TAG          5706-5708

[0043] Deletion mutations in the BRCA1 gene containing a truncating mutation may also be defined as having the sequence 5′ R1-R2 3′; where R1 is the wild type BRCA1 DNA sequence from nucleotide number 120 to X; R2 contains the wild type BRCA1 DNA sequence from nucleotide number X+Y+1 to 5571, where Y=3n+1 or 3n+2 where n is an integer of zero or greater; and where X=123 to 5707.

[0044] Alternatively, the mutations may be defined as being specifically hybridizable to an oligonucleotide probe being at least 12 nucleotides in length and having the sequence 5′ R1-R2 3′; where R1 contains at its 3′ end three nucleotides complementary to nucleotide numbers X-2, X-1 and X the wild-type BRCA1 gene; R2 contains at its 5′ end three nucleotides complementary to nucleotide numbers X+Y+1, X+Y+2, and X+Y+3 of the wild type BRCA1 DNA sequence; where Y=3n+1 or 3n+2 where n is an integer of zero or greater; and where X=122 to 5706.

[0045] Single base insertions of the present invention form stop codons as indicated in the following TABLE 6. The formed in-frame stop codon and the location are provided. Any expressed protein from BRCA1 genes with these types of mutations should be truncated accordingly. It should be recognized that the present invention includes insertions of 3n+1 bases, where n is an integer greater than zero and less than 1861. These larger insertions mutations have stop codons at nucleotide numbers corresponding to the listed stop codon at the nucleotide number listed. The corresponding nucleotide numbers of the stop codons will be 3n nucleotides larger than those listed.

TABLE 6
Single Base Insertions
Codon Formed Nucleotide Number
TGA          144-146
TGA          123-125
TGA          144-146
TAA          165-167
TGA          144-146
TAA          165-167
TGA          147-149
TAA          165-167
TAA          156-158
TAA          165-167
TGA          237-239
TAA          165-167
TGA          237-239
TAA          177-179
TGA          237-239
TGA          186-188
TGA          237-239
TAG          189-191
TGA          237-239
TGA          189-191
TGA          237-239
TAG          198-200
TGA          237-239
TGA          198-200
TGA          237-239
TGA          204-206
TGA          237-239
TAA          213-215
TGA          237-239
TGA          216-218
TGA          237-239
TAA          231-233
TGA          237-239
TAG          234-236
TGA          237-239
TGA          234-236
TGA          237-239
TAA          312-314
TGA          237-239
TAA          312-314
TAG          249-251
TAA          312-314
TGA          249-251
TAA          312-314
TAA          252-254
TAA          312-314
TAG          258-260
TAA          312-314
TGA          258-260
TAA          312-314
TAA          267-269
TAA          312-314
TAA          276-278
TAA          312-314
TAA          282-284
TAA          312-314
TAA          285-287
TAA          312-314
TAG          300-302
TAA          312-314
TGA          300-302
TAA          312-314
TAG          309-311
TAA          312-314
TGA          309-311
TAA          312-314
TGA          318-320
TAA          312-314
TGA          318-320
TAA          315-317
TGA          318-320
TAG          357-359
TGA          318-320
TAG          357-359
TAA          327-329
TAG          357-359
TAG          330-332
TAG          357-359
TAG          333-335
TAG          357-359
TGA          342-344
TAG          357-359
TAG          345-347
TAG          357-359
TAG          351-353
TAG          357-359
TGA          369-371
TAG          357-359
TGA          369-371
TGA          405-407
TGA          369-371
TGA          405-407
TGA          372-374
TGA          405-407
TAA          381-383
TGA          405-407
TAG          390-392
TGA          405-407
TGA          390-392
TGA          405-407
TAA          435-437
TGA          405-407
TAA          435-437
TGA          417-419
TAA          435-437
TAA          420-422
TAA          435-437
TGA          420-422
TAA          435-437
TAA          420-422
TAA          435-437
TAG          420-422
TAA          435-437
TAA          426-428
TAA          435-437
TAG          429-431
TAA          435-437
TAA          432-434
TAA          435-437
TGA          432-434
TAA          435-437
TAA          432-434
TAA          435-437
TAG          432-434
TAA          435-437
TAA          456-458
TAA          435-437
TAA          456-458
TAA          444-446
TAA          456-458
TAA          447-449
TAA          456-458
TGA          450-452
TAA          456-458
TAA          453-455
TAA          456-458
TGA          465-467
TAA          456-458
TGA          465-467
TGA          480-482
TGA          465-467
TGA          480-482
TAA          474-476
TGA          480-482
TGA          477-479
TGA          480-482
TGA          540-542
TGA          480-482
TGA          540-542
TAG          498-500
TGA          540-542
TAA          507-509
TGA          540-542
TGA          507-509
TGA          540-542
TAA          507-509
TGA          540-542
TAG          507-509
TGA          540-542
TAG          510-512
TGA          540-542
TAA          513-515
TGA          540-542
TAA          522-524
TGA          540-542
TAG          525-527
TGA          540-542
TAG          537-539
TGA          540-542
TAA          591-593
TGA          540-542
TAA          591-593
TGA          546-548
TAA          591-593
TAA          549-551
TAA          591-593
TGA          564-566
TAA          591-593
TAG          570-572
TAA          591-593
TAG          576-578
TAA          591-593
TGA          660-662
TAA          591-593
TGA          660-662
TAG          606-608
TGA          660-662
TAG          615-617
TGA          660-662
TAA          621-623
TGA          660-662
TAA          642-644
TGA          660-662
TAA          654-656
TGA          660-662
TGA          654-656
TGA          660-662
TAA          654-656
TGA          660-662
TAG          654-656
TGA          660-662
TGA          672-674
TGA          660-662
TGA          672-674
TGA          681-683
TGA          672-674
TGA          681-683
TAA          693-695
TGA          681-683
TAA          693-695
TGA          684-686
TAA          693-695
TAA          696-698
TAA          693-695
TAA          696-698
TGA          762-764
TAA          696-698
TGA          762-764
TAA          705-707
TGA          762-764
TGA          705-707
TGA          762-764
TAA          705-707
TGA          762-764
TAG          705-707
TGA          762-764
TAG          708-710
TGA          762-764
TGA          708-710
TGA          762-764
TAG          711-713
TGA          762-764
TGA          720-722
TGA          762-764
TGA          726-728
TGA          762-764
TAG          756-758
TGA          762-764
TGA          759-761
TGA          762-764
TGA          798-800
TGA          762-764
TGA          798-800
TAG          768-770
TGA          798-800
TGA          774-776
TGA          798-800
TAA          783-785
TGA          798-800
TAA          786-788
TGA          798-800
TAG          795-797
TGA          798-800
TGA          795-797
TGA          798-800
TGA          807-809
TGA          798-800
TGA          807-809
TGA          828-830
TGA          807-809
TGA          828-830
TGA          813-815
TGA          828-830
TAA          822-824
TGA          828-830
TAA          846-848
TGA          828-830
TAA          846-848
TAG          843-845
TAA          846-848
TAA          849-851
TAA          846-848
TAA          849-851
TGA          852-854
TAA          849-851
TGA          852-854
TGA          867-869
TGA          852-854
TGA          867-869
TAA          858-860
TGA          867-869
TGA          882-884
TGA          867-869
TGA          882-884
TAA          870-872
TGA          882-884
TAG          909-911
TGA          882-884
TAG          909-911
TAG          885-887
TAG          909-911
TGA          894-896
TAG          909-911
TAA          897-899
TAG          909-911
TAA          900-902
TAG          909-911
TGA          900-902
TAG          909-911
TAA          900-902
TAG          909-911
TAG          900-902
TAG          909-911
TGA          975-977
TAG          909-911
TGA          975-977
TAA          921-923
TGA          975-977
TGA          933-935
TGA          975-977
TAG          939-941
TGA          975-977
TGA          939-941
TGA          975-977
TAA          948-950
TGA          975-977
TAG          960-962
TGA          975-977
TAA          999-1001
TGA          975-977
TAA          999-1001
TAA          978-980
TAA          999-1001
TAG          981-983
TAA          999-1001
TAG          984-986
TAA          999-1001
TGA          1026-1028
TAA          999-1001
TGA          1026-1028
TGA          1002-1004
TGA          1026-1028
TAG          1005-1007
TGA          1026-1028
TAA          1011-1013
TGA          1026-1028
TGA          1017-1019
TGA          1026-1028
TAA          1020-1022
TGA          1026-1028
TAA          1035-1037
TGA          1026-1028
TAA          1035-1037
TAG          1032-1034
TAA          1035-1037
TGA          1032-1034
TAA          1035-1037
TAA          1038-1040
TAA          1035-1037
TAA          1038-1040
TAA          1074-1076
TAA          1038-1040
TAA          1074-1076
TAG          1041-1043
TAA          1074-1076
TAA          1044-1046
TAA          1074-1076
TAG          1062-1064
TAA          1074-1076
TAG          1065-1067
TAA          1074-1076
TAA          1092-1094
TAA          1074-1076
TAA          1092-1094
TAG          1077-1079
TAA          1092-1094
TAG          1080-1082
TAA          1092-1094
TGA          1080-1082
TAA          1092-1094
TAG          1089-1091
TAA          1092-1094
TAA          1104-1106
TAA          1092-1094
TAA          1104-1106
TGA          1095-1097
TAA          1104-1106
TAG          1101-1103
TAA          1104-1106
TGA          1101-1103
TAA          1104-1106
TGA          1107-1109
TAA          1104-1106
TGA          1107-1109
TAG          1110-1112
TGA          1107-1109
TAG          1110-1112
TGA          1152-1154
TAG          1110-1112
TGA          1152-1154
TAG          1122-1124
TGA          1152-1154
TGA          1128-1130
TGA          1152-1154
TAA          1131-1133
TGA          1152-1154
TAA          1134-1136
TGA          1152-1154
TGA          1140-1142
TGA          1152-1154
TAA          1146-1148
TGA          1152-1154
TGA          1164-1166
TGA          1152-1154
TGA          1164-1166
TAG          1161-1163
TGA          1164-1166
TGA          1161-1163
TGA          1164-1166
TAA          1182-1184
TGA          1164-1166
TAA          1182-1184
TAG          1167-1169
TAA          1182-1184
TAA          1170-1172
TAA          1182-1184
TGA          1173-1175
TAA          1182-1184
TAG          1176-1178
TAA          1182-1184
TGA          1176-1178
TAA          1182-1184
TAA          1179-1181
TAA          1182-1184
TAG          1212-1214
TAA          1182-1184
TAG          1212-1214
TAA          1188-1190
TAG          1212-1214
TAG          1197-1199
TAG          1212-1214
TGA          1197-1199
TAG          1212-1214
TGA          1203-1205
TAG          1212-1214
TAA          1206-1208
TAG          1212-1214
TGA          1221-1223
TAG          1212-1214
TGA          1221-1223
TGA          1215-1217
TGA          1221-1223
TAG          1248-1250
TGA          1221-1223
TAG          1248-1250
TGA          1224-1226
TAG          1248-1250
TAG          1233-1235
TAG          1248-1250
TGA          1233-1235
TAG          1248-1250
TAA          1245-1247
TAG          1248-1250
TAA          1266-1268
TAG          1248-1250
TAA          1266-1268
TAG          1251-1253
TAA          1266-1268
TAA          1260-1262
TAA          1266-1268
TGA          1269-1271
TAA          1266-1268
TGA          1269-1271
TGA          1287-1289
TGA          1269-1271
TGA          1287-1289
TAG          1272-1274
TGA          1287-1289
TGA          1272-1274
TGA          1287-1289
TAG          1281-1283
TGA          1287-1289
TAG          1284-1286
TGA          1287-1289
TGA          1290-1292
TGA          1287-1289
TGA          1290-1292
TGA          1305-1307
TGA          1290-1292
TGA          1305-1307
TGA          1308-1310
TGA          1305-1307
TGA          1308-1310
TGA          1317-1319
TGA          1308-1310
TGA          1317-1319
TGA          1329-1331
TGA          1317-1319
TGA          1329-1331
TGA          1323-1325
TGA          1329-1331
TGA          1350-1352
TGA          1329-1331
TGA          1350-1352
TAA          1335-1337
TGA          1350-1352
TAA          1341-1343
TGA          1350-1352
TGA          1371-1373
TGA          1350-1352
TGA          1371-1373
TGA          1359-1361
TGA          1371-1373
TAA          1368-1370
TGA          1371-1373
TGA          1380-1382
TGA          1371-1373
TGA          1380-1382
TGA          1377-1379
TGA          1380-1382
TGA          1422-1424
TGA          1380-1382
TGA          1422-1424
TAA          1383-1385
TGA          1422-1424
TGA          1383-1385
TGA          1422-1424
TAA          1383-1385
TGA          1422-1424
TAG          1383-1385
TGA          1422-1424
TGA          1398-1400
TGA          1422-1424
TAA          1401-1403
TGA          1422-1424
TGA          1407-1409
TGA          1422-1424
TAG          1419-1421
TGA          1422-1424
TGA          1431-1433
TGA          1422-1424
TGA          1431-1433
TAA          1446-1448
TGA          1431-1433
TAA          1446-1448
TAG          1443-1445
TAA          1446-1448
TGA          1443-1445
TAA          1446-1448
TGA          1452-1454
TAA          1446-1448
TGA          1452-1454
TAG          1449-1451
TGA          1452-1454
TAA          1482-1484
TGA          1452-1454
TAA          1482-1484
TAG          1455-1457
TAA          1482-1484
TAA          1467-1469
TAA          1482-1484
TGA          1476-1478
TAA          1482-1484
TAG          1479-1481
TAA          1482-1484
TGA          1488-1490
TAA          1482-1484
TGA          1488-1490
TGA          1554-1556
TGA          1488-1490
TGA          1554-1556
TGA          1491-1493
TGA          1554-1556
TAA          1494-1496
TGA          1554-1556
TAA          1506-1508
TGA          1554-1556
TAA          1512-1514
TGA          1554-1556
TGA          1512-1514
TGA          1554-1556
TAA          1512-1514
TGA          1554-1556
TAG          1512-1514
TGA          1554-1556
TAA          1518-1520
TGA          1554-1556
TAA          1521-1523
TGA          1554-1556
TAG          1527-1529
TGA          1554-1556
TAA          1536-1538
TGA          1554-1556
TAG          1542-1544
TGA          1554-1556
TGA          1584-1586
TGA          1554-1556
TGA          1584-1586
TAA          1557-1559
TGA          1584-1586
TAA          1620-1622
TGA          1584-1586
TAA          1620-1622
TGA          1602-1604
TAA          1620-1622
TAA          1617-1619
TAA          1620-1622
TAA          1632-1634
TAA          1620-1622
TAA          1632-1634
TAA          1626-1628
TAA          1632-1634
TGA          1662-1664
TAA          1632-1634
TGA          1662-1664
TAG          1635-1637
TGA          1662-1664
TAG          1638-1640
TGA          1662-1664
TGA          1707-1709
TGA          1662-1664
TGA          1707-1709
TGA          1665-1667
TGA          1707-1709
TAA          1674-1676
TGA          1707-1709
TAA          1677-1679
TGA          1707-1709
TGA          1683-1685
TGA          1707-1709
TAA          1698-1700
TGA          1707-1709
TAA          1728-1730
TGA          1707-1709
TAA          1728-1730
TAA          1716-1718
TAA          1728-1730
TAA          1767-1769
TAA          1728-1730
TAA          1767-1769
TGA          1737-1739
TAA          1767-1769
TAA          1743-1745
TAA          1767-1769
TAA          1758-1760
TAA          1767-1769
TAG          1770-1772
TAA          1767-1769
TAG          1770-1772
TGA          1779-1781
TAG          1770-1772
TGA          1779-1781
TAA          1785-1787
TGA          1779-1781
TAA          1785-1787
TAA          1782-1784
TAA          1785-1787
TGA          1797-1799
TAA          1785-1787
TGA          1797-1799
TAA          1791-1793
TGA          1797-1799
TGA          1812-1814
TGA          1797-1799
TGA          1812-1814
TAA          1809-1811
TGA          1812-1814
TAA          1824-1826
TGA          1812-1814
TAA          1824-1826
TAA          1815-1817
TAA          1824-1826
TAA          1818-1820
TAA          1824-1826
TGA          1872-1874
TAA          1824-1826
TGA          1872-1874
TGA          1833-1835
TGA          1872-1874
TGA          1842-1844
TGA          1872-1874
TAA          1845-1847
TGA          1872-1874
TGA          1848-1850
TGA          1872-1874
TAA          1860-1862
TGA          1872-1874
TAA          1866-1868
TGA          1872-1874
TAA          1938-1940
TGA          1872-1874
TAA          1938-1940
TAG          1881-1883
TAA          1938-1940
TAG          1884-1886
TAA          1938-1940
TAG          1887-1889
TAA          1938-1940
TAG          1893-1895
TAA          1938-1940
TAA          1896-1898
TAA          1938-1940
TGA          1902-1904
TAA          1938-1940
TGA          1908-1910
TAA          1938-1940
TAA          1914-1916
TAA          1938-1940
TAA          1923-1925
TAA          1938-1940
TAA          1929-1931
TAA          1938-1940
TAG          1947-1949
TAA          1938-1940
TAG          1947-1949
TAA          1941-1943
TAG          1947-1949
TAA          1944-1946
TAG          1947-1949
TGA          1989-1991
TAG          1947-1949
TGA          1989-1991
TAG          1953-1955
TGA          1989-1991
TAG          1956-1958
TGA          1989-1991
TAA          1959-1961
TGA          1989-1991
TAG          1971-1973
TGA          1989-1991
TAG          2004-2006
TGA          1989-1991
TAG          2004-2006
TAG          2001-2003
TAG          2004-2006
TAA          2022-2024
TAG          2004-2006
TAA          2022-2024
TAA          2007-2009
TAA          2022-2024
TAG          2013-2015
TAA          2022-2024
TGA          2031-2033
TAA          2022-2024
TGA          2031-2033
TAG          2025-2027
TGA          2031-2033
TGA          2025-2027
TGA          2031-2033
TGA          2043-2045
TGA          2031-2033
TGA          2043-2045
TAG          2046-2048
TGA          2043-2045
TAG          2046-2048
TAG          2055-2057
TAG          2046-2048
TAG          2055-2057
TAG          2049-2051
TAG          2055-2057
TGA          2049-2051
TAG          2055-2057
TGA          2061-2063
TAG          2055-2057
TGA          2061-2063
TAG          2058-2060
TGA          2061-2063
TAA          2133-2135
TGA          2061-2063
TAA          2133-2135
TGA          2064-2066
TAA          2133-2135
TAA          2070-2072
TAA          2133-2135
TAA          2073-2075
TAA          2133-2135
TAA          2076-2078
TAA          2133-2135
TAA          2079-2081
TAA          2133-2135
TAA          2082-2084
TAA          2133-2135
TGA          2082-2084
TAA          2133-2135
TAA          2082-2084
TAA          2133-2135
TAG          2082-2084
TAA          2133-2135
TAA          2085-2087
TAA          2133-2135
TAG          2100-2102
TAA          2133-2135
TAG          2106-2108
TAA          2133-2135
TAG          2109-2111
TAA          2133-2135
TAA          2112-2114
TAA          2133-2135
TGA          2127-2129
TAA          2133-2135
TAA          2163-2165
TAA          2133-2135
TAA          2163-2165
TGA          2136-2138
TAA          2163-2165
TAA          2154-2156
TAA          2163-2165
TAA          2157-2159
TAA          2163-2165
TAG          2160-2162
TAA          2163-2165
TGA          2175-2177
TAA          2163-2165
TGA          2175-2177
TAA          2166-2168
TGA          2175-2177
TAA          2172-2174
TGA          2175-2177
TAA          2187-2189
TGA          2175-2177
TAA          2187-2189
TAG          2184-2186
TAA          2187-2189
TGA          2196-2198
TAA          2187-2189
TGA          2196-2198
TAG          2190-2192
TGA          2196-2198
TGA          2202-2204
TGA          2196-2198
TGA          2202-2204
TAG          2199-2201
TGA          2202-2204
TAA          2250-2252
TGA          2202-2204
TAA          2250-2252
TGA          2214-2216
TAA          2250-2252
TAA          2220-2222
TAA          2250-2252
TAA          2229-2231
TAA          2250-2252
TGA          2268-2270
TAA          2250-2252
TGA          2268-2270
TAG          2253-2255
TGA          2268-2270
TGA          2253-2255
TGA          2268-2270
TAA          2259-2261
TGA          2268-2270
TAG          2265-2267
TGA          2268-2270
TAA          2274-2276
TGA          2268-2270
TAA          2274-2276
TAG          2292-2294
TAA          2274-2276
TAG          2292-2294
TGA          2277-2279
TAG          2292-2294
TAA          2286-2288
TAG          2292-2294
TAA          2334-2336
TAG          2292-2294
TAA          2334-2336
TAG          2301-2303
TAA          2334-2336
TGA          2304-2306
TAA          2334-2336
TGA          2307-2309
TAA          2334-2336
TAA          2310-2312
TAA          2334-2336
TGA          2313-2315
TAA          2334-2336
TGA          2316-2318
TAA          2334-2336
TAA          2319-2321
TAA          2334-2336
TGA          2325-2327
TAA          2334-2336
TAA          2343-2345
TAA          2334-2336
TAA          2343-2345
TAA          2346-2348
TAA          2343-2345
TAA          2346-2348
TGA          2352-2354
TAA          2346-2348
TGA          2352-2354
TGA          2400-2402
TGA          2352-2354
TGA          2400-2402
TGA          2355-2357
TGA          2400-2402
TAA          2361-2363
TGA          2400-2402
TGA          2364-2366
TGA          2400-2402
TAG          2376-2378
TGA          2400-2402
TGA          2382-2384
TGA          2400-2402
TAG          2385-2387
TGA          2400-2402
TAG          2418-2420
TGA          2400-2402
TAG          2418-2420
TAG          2403-2405
TAG          2418-2420
TGA          2412-2414
TAG          2418-2420
TAG          2415-2417
TAG          2418-2420
TGA          2445-2447
TAG          2418-2420
TGA          2445-2447
TAG          2421-2423
TGA          2445-2447
TAG          2484-2486
TGA          2445-2447
TAG          2484-2486
TAA          2448-2450
TAG          2484-2486
TGA          2448-2450
TAG          2484-2486
TAA          2448-2450
TAG          2484-2486
TAG          2448-2450
TAG          2484-2486
TGA          2460-2462
TAG          2484-2486
TAG          2463-2465
TAG          2484-2486
TGA          2478-2480
TAG          2484-2486
TAA          2517-2519
TAG          2484-2486
TAA          2517-2519
TAA          2496-2498
TAA          2517-2519
TAA          2502-2504
TAA          2517-2519
TGA          2508-2510
TAA          2517-2519
TAA          2514-2516
TAA          2517-2519
TGA          2544-2546
TAA          2517-2519
TGA          2544-2546
TAG          2520-2522
TGA          2544-2546
TGA          2520-2522
TGA          2544-2546
TAG          2526-2528
TGA          2544-2546
TAG          2532-2534
TGA          2544-2546
TGA          2532-2534
TGA          2544-2546
TAA          2583-2585
TGA          2544-2546
TAA          2583-2585
TAA          2547-2549
TAA          2583-2585
TAA          2553-2555
TAA          2583-2585
TAG          2571-2573
TAA          2583-2585
TGA          2571-2573
TAA          2583-2585
TAA          2577-2579
TAA          2583-2585
TGA          2580-2582
TAA          2583-2585
TAG          2586-2588
TAA          2583-2585
TAG          2586-2588
TGA          2592-2594
TAG          2586-2588
TGA          2592-2594
TAA          2589-2591
TGA          2592-2594
TAA          2607-2609
TGA          2592-2594
TAA          2607-2609
TGA          2598-2600
TAA          2607-2609
TGA          2625-2627
TAA          2607-2609
TGA          2625-2627
TAA          2610-2612
TGA          2625-2627
TGA          2610-2612
TGA          2625-2627
TAA          2610-2612
TGA          2625-2627
TAG          2610-2612
TGA          2625-2627
TAA          2631-2633
TGA          2625-2627
TAA          2631-2633
TGA          2670-2672
TAA          2631-2633
TGA          2670-2672
TAG          2637-2639
TGA          2670-2672
TGA          2643-2645
TGA          2670-2672
TAG          2649-2651
TGA          2670-2672
TGA          2655-2657
TGA          2670-2672
TGA          2661-2663
TGA          2670-2672
TGA          2664-2666
TGA          2670-2672
TAG          2667-2669
TGA          2670-2672
TGA          2676-2678
TGA          2670-2672
TGA          2676-2678
TGA          2823-2825
TGA          2676-2678
TGA          2823-2825
TAA          2685-2687
TGA          2823-2825
TGA          2685-2687
TGA          2823-2825
TAA          2685-2687
TGA          2823-2825
TAG          2685-2687
TGA          2823-2825
TAA          2694-2696
TGA          2823-2825
TAA          2703-2705
TGA          2823-2825
TAA          2712-2714
TGA          2823-2825
TAA          2739-2741
TGA          2823-2825
TAA          2748-2750
TGA          2823-2825
TGA          2754-2756
TGA          2823-2825
TGA          2757-2759
TGA          2823-2825
TGA          2760-2762
TGA          2823-2825
TAG          2763-2765
TGA          2823-2825
TGA          2763-2765
TGA          2823-2825
TAA          2796-2798
TGA          2823-2825
TAA          2799-2801
TGA          2823-2825
TAG          2805-2807
TGA          2823-2825
TAA          2811-2813
TGA          2823-2825
TGA          2829-2831
TGA          2823-2825
TGA          2829-2831
TAG          2826-2828
TGA          2829-2831
TGA          2826-2828
TGA          2829-2831
TGA          2859-2861
TGA          2829-2831
TGA          2859-2861
TAA          2835-2837
TGA          2859-2861
TGA          2838-2840
TGA          2859-2861
TGA          2841-2843
TGA          2859-2861
TAA          2844-2846
TGA          2859-2861
TAA          2853-2855
TGA          2859-2861
TAA          2856-2858
TGA          2859-2861
TAA          2865-2867
TGA          2859-2861
TAA          2865-2867
TAA          2889-2891
TAA          2865-2867
TAA          2889-2891
TAA          2871-2873
TAA          2889-2891
TAA          2928-2930
TAA          2889-2891
TAA          2928-2930
TAA          2922-2924
TAA          2928-2930
TGA          2925-2927
TAA          2928-2930
TGA          2937-2939
TAA          2928-2930
TGA          2937-2939
TAA          2940-2942
TGA          2937-2939
TAA          2940-2942
TAG          2952-2954
TAA          2940-2942
TAG          2952-2954
TAA          2946-2948
TAG          2952-2954
TAG          2949-2951
TAG          2952-2954
TGA          2949-2951
TAG          2952-2954
TAG          2970-2972
TAG          2952-2954
TAG          2970-2972
TAA          2958-2960
TAG          2970-2972
TAA          3027-3029
TAG          2970-2972
TAA          3027-3029
TAG          2976-2978
TAA          3027-3029
TGA          2976-2978
TAA          3027-3029
TAG          2994-2996
TAA          3027-3029
TAA          3000-3002
TAA          3027-3029
TGA          3003-3005
TAA          3027-3029
TAA          3024-3026
TAA          3027-3029
TAA          3090-3092
TAA          3027-3029
TAA          3090-3092
TAA          3045-3047
TAA          3090-3092
TAA          3051-3053
TAA          3090-3092
TGA          3051-3053
TAA          3090-3092
TAA          3051-3053
TAA          3090-3092
TAG          3051-3053
TAA          3090-3092
TAA          3078-3080
TAA          3090-3092
TAA          3096-3098
TAA          3090-3092
TAA          3096-3098
TAA          3102-3104
TAA          3096-3098
TAA          3102-3104
TAG          3099-3101
TAA          3102-3104
TGA          3099-3101
TAA          3102-3104
TGA          3129-3131
TAA          3102-3104
TGA          3129-3131
TAA          3105-3107
TGA          3129-3131
TAA          3108-3110
TGA          3129-3131
TGA          3117-3119
TGA          3129-3131
TGA          3120-3122
TGA          3129-3131
TAA          3123-3125
TGA          3129-3131
TGA          3150-3152
TGA          3129-3131
TGA          3150-3152
TGA          3132-3134
TGA          3150-3152
TGA          3168-3170
TGA          3150-3152
TGA          3168-3170
TAG          3153-3155
TGA          3168-3170
TGA          3156-3158
TGA          3168-3170
TAA          3165-3167
TGA          3168-3170
TAG          3198-3200
TGA          3168-3170
TAG          3198-3200
TAA          3171-3173
TAG          3198-3200
TAG          3180-3182
TAG          3198-3200
TAG          3189-3191
TAG          3198-3200
TAA          3204-3206
TAG          3198-3200
TAA          3204-3206
TAA          3207-3209
TAA          3204-3206
TAA          3207-3209
TAG          3213-3215
TAA          3207-3209
TAG          3213-3215
TAA          3228-3230
TAG          3213-3215
TAA          3228-3230
TGA          3216-3218
TAA          3228-3230
TAA          3219-3221
TAA          3228-3230
TAA          3252-3254
TAA          3228-3230
TAA          3252-3254
TAG          3237-3239
TAA          3252-3254
TAG          3243-3245
TAA          3252-3254
TAA          3246-3248
TAA          3252-3254
TGA          3255-3257
TAA          3252-3254
TGA          3255-3257
TAA          3273-3275
TGA          3255-3257
TAA          3273-3275
TAG          3267-3269
TAA          3273-3275
TGA          3276-3278
TAA          3273-3275
TGA          3276-3278
TAA          3294-3296
TGA          3276-3278
TAA          3294-3296
TAG          3288-3290
TAA          3294-3296
TGA          3297-3299
TAA          3294-3296
TGA          3297-3299
TGA          3312-3314
TGA          3297-3299
TGA          3312-3314
TAG          3309-3311
TGA          3312-3314
TGA          3315-3317
TGA          3312-3314
TGA          3315-3317
TAG          3339-3341
TGA          3315-3317
TAG          3339-3341
TAA          3318-3320
TAG          3339-3341
TGA          3330-3332
TAG          3339-3341
TAG          3372-3374
TAG          3339-3341
TAG          3372-3374
TAA          3342-3344
TAG          3372-3374
TAG          3345-3347
TAG          3372-3374
TAA          3354-3356
TAG          3372-3374
TAA          3360-3362
TAG          3372-3374
TGA          3393-3395
TAG          3372-3374
TGA          3393-3395
TAA          3402-3404
TGA          3393-3395
TAA          3402-3404
TAA          3399-3401
TAA          3402-3404
TGA          3399-3401
TAA          3402-3404
TAA          3399-3401
TAA          3402-3404
TAG          3399-3401
TAA          3402-3404
TAA          3423-3425
TAA          3402-3404
TAA          3423-3425
TAG          3408-3410
TAA          3423-3425
TAG          3420-3422
TAA          3423-3425
TAA          3429-3431
TAA          3423-3425
TAA          3429-3431
TAG          3426-3428
TAA          3429-3431
TGA          3426-3428
TAA          3429-3431
TGA          3438-3440
TAA          3429-3431
TGA          3438-3440
TGA          3459-3461
TGA          3438-3440
TGA          3459-3461
TAA          3444-3446
TGA          3459-3461
TAA          3447-3449
TGA          3459-3461
TGA          3453-3455
TGA          3459-3461
TAA          3456-3458
TGA          3459-3461
TGA          3456-3458
TGA          3459-3461
TAA          3456-3458
TGA          3459-3461
TAG          3456-3458
TGA          3459-3461
TAA          3480-3482
TGA          3459-3461
TAA          3480-3482
TGA          3462-3464
TAA          3480-3482
TAA          3513-3515
TAA          3480-3482
TAA          3513-3515
TGA          3486-3488
TAA          3513-3515
TAA          3498-3500
TAA          3513-3515
TGA          3498-3500
TAA          3513-3515
TAA          3498-3500
TAA          3513-3515
TAG          3498-3500
TAA          3513-3515
TGA          3510-3512
TAA          3513-3515
TAG          3537-3539
TAA          3513-3515
TAG          3537-3539
TGA          3519-3521
TAG          3537-3539
TAG          3534-3536
TAG          3537-3539
TGA          3561-3563
TAG          3537-3539
TGA          3561-3563
TAG          3555-3557
TGA          3561-3563
TGA          3555-3557
TGA          3561-3563
TGA          3570-3572
TGA          3561-3563
TGA          3570-3572
TGA          3573-3575
TGA          3570-3572
TGA          3573-3575
TGA          3585-3587
TGA          3573-3575
TGA          3585-3587
TGA          3582-3584
TGA          3585-3587
TGA          3591-3593
TGA          3585-3587
TGA          3591-3593
TAG          3609-3611
TGA          3591-3593
TAG          3609-3611
TAA          3597-3599
TAG          3609-3611
TGA          3600-3602
TAG          3609-3611
TGA          3603-3605
TAG          3609-3611
TGA          3618-3620
TAG          3609-3611
TGA          3618-3620
TGA          3624-3626
TGA          3618-3620
TGA          3624-3626
TAA          3621-3623
TGA          3624-3626
TAA          3630-3632
TGA          3624-3626
TAA          3630-3632
TAG          3651-3653
TAA          3630-3632
TAG          3651-3653
TGA          3633-3635
TAG          3651-3653
TAG          3636-3638
TAG          3651-3653
TAG          3678-3680
TAG          3651-3653
TAG          3678-3680
TAA          3654-3656
TAG          3678-3680
TAG          3657-3659
TAG          3678-3680
TAG          3666-3668
TAG          3678-3680
TGA          3672-3674
TAG          3678-3680
TAG          3690-3692
TAG          3678-3680
TAG          3690-3692
TAG          3681-3683
TAG          3690-3692
TAG          3684-3686
TAG          3690-3692
TAG          3771-3773
TAG          3690-3692
TAG          3771-3773
TAA          3723-3725
TAG          3771-3773
TGA          3723-3725
TAG          3771-3773
TAA          3723-3725
TAG          3771-3773
TAG          3723-3725
TAG          3771-3773
TAG          3729-3731
TAG          3771-3773
TAA          3738-3740
TAG          3771-3773
TAA          3741-3743
TAG          3771-3773
TGA          3747-3749
TAG          3771-3773
TGA          3756-3758
TAG          3771-3773
TGA          3759-3761
TAG          3771-3773
TAA          3762-3764
TAG          3771-3773
TGA          3774-3776
TAG          3771-3773
TGA          3774-3776
TGA          3780-3782
TGA          3774-3776
TGA          3780-3782
TGA          3777-3779
TGA          3780-3782
TAA          3816-3818
TGA          3780-3782
TAA          3816-3818
TGA          3783-3785
TAA          3816-3818
TAG          3792-3794
TAA          3816-3818
TGA          3792-3794
TAA          3816-3818
TAG          3846-3848
TAA          3816-3818
TAG          3846-3848
TAA          3822-3824
TAG          3846-3848
TAA          3825-3827
TAG          3846-3848
TAG          3852-3854
TAG          3846-3848
TAG          3852-3854
TAA          3879-3881
TAG          3852-3854
TAA          3879-3881
TGA          3867-3869
TAA          3879-3881
TAG          3870-3872
TAA          3879-3881
TGA          3870-3872
TAA          3879-3881
TAG          3915-3917
TAA          3879-3881
TAG          3915-3917
TAA          3882-3884
TAG          3915-3917
TGA          3888-3890
TAG          3915-3917
TGA          3891-3893
TAG          3915-3917
TAA          3894-3896
TAG          3915-3917
TAA          3909-3911
TAG          3915-3917
TAA          3912-3914
TAG          3915-3917
TGA          3924-3926
TAG          3915-3917
TGA          3924-3926
TAA          3921-3923
TGA          3924-3926
TAA          3933-3935
TGA          3924-3926
TAA          3933-3935
TAG          3927-3929
TAA          3933-3935
TGA          3927-3929
TAA          3933-3935
TAG          3930-3932
TAA          3933-3935
TAG          3975-3977
TAA          3933-3935
TAG          3975-3977
TAA          3951-3953
TAG          3975-3977
TGA          3963-3965
TAG          3975-3977
TGA          3978-3980
TAG          3975-3977
TGA          3978-3980
TAG          3999-4001
TGA          3978-3980
TAG          3999-4001
TGA          3981-3983
TAG          3999-4001
TAA          3987-3989
TAG          3999-4001
TAG          3990-3992
TAG          3999-4001
TGA          3990-3992
TAG          3999-4001
TGA          4023-4025
TAG          3999-4001
TGA          4023-4025
TAG          4017-4019
TGA          4023-4025
TGA          4017-4019
TGA          4023-4025
TAG          4020-4022
TGA          4023-4025
TGA          4104-4106
TGA          4023-4025
TGA          4104-4106
TGA          4029-4031
TGA          4104-4106
TGA          4032-4034
TGA          4104-4106
TAA          4044-4046
TGA          4104-4106
TAA          4050-4052
TGA          4104-4106
TGA          4059-4061
TGA          4104-4106
TAA          4083-4085
TGA          4104-4106
TAG          4092-4094
TGA          4104-4106
TGA          4128-4130
TGA          4104-4106
TGA          4128-4130
TAG          4107-4109
TGA          4128-4130
TAG          4125-4127
TGA          4128-4130
TGA          4149-4151
TGA          4128-4130
TGA          4149-4151
TAA          4131-4133
TGA          4149-4151
TGA          4134-4136
TGA          4149-4151
TGA          4146-4148
TGA          4149-4151
TGA          4152-4154
TGA          4149-4151
TGA          4152-4154
TAA          4182-4184
TGA          4152-4154
TAA          4182-4184
TGA          4155-4157
TAA          4182-4184
TAG          4158-4160
TAA          4182-4184
TGA          4173-4175
TAA          4182-4184
TGA          4176-4178
TAA          4182-4184
TAA          4179-4181
TAA          4182-4184
TGA          4218-4220
TAA          4182-4184
TGA          4218-4220
TGA          4188-4190
TGA          4218-4220
TGA          4191-4193
TGA          4218-4220
TAG          4197-4199
TGA          4218-4220
TGA          4203-4205
TGA          4218-4220
TAA          4209-4211
TGA          4218-4220
TGA          4236-4238
TGA          4218-4220
TGA          4236-4238
TAG          4233-4235
TGA          4236-4238
TGA          4233-4235
TGA          4236-4238
TGA          4242-4244
TGA          4236-4238
TGA          4242-4244
TAG          4239-4241
TGA          4242-4244
TGA          4257-4259
TGA          4242-4244
TGA          4257-4259
TAG          4248-4250
TGA          4257-4259
TGA          4287-4289
TGA          4257-4259
TGA          4287-4289
TGA          4260-4262
TGA          4287-4289
TAG          4263-4265
TGA          4287-4289
TGA          4263-4265
TGA          4287-4289
TAG          4284-4286
TGA          4287-4289
TAA          4326-4328
TGA          4287-4289
TAA          4326-4328
TAG          4308-4310
TAA          4326-4328
TGA          4311-4313
TAA          4326-4328
TGA          4356-4358
TAA          4326-4328
TGA          4356-4358
TAA          4335-4337
TGA          4356-4358
TGA          4347-4349
TGA          4356-4358
TAA          4398-4400
TGA          4356-4358
TAA          4398-4400
TGA          4362-4364
TAA          4398-4400
TGA          4374-4376
TAA          4398-4400
TAG          4386-4388
TAA          4398-4400
TGA          4422-4424
TAA          4398-4400
TGA          4422-4424
TAG          4401-4403
TGA          4422-4424
TAA          4404-4406
TGA          4422-4424
TGA          4404-4406
TGA          4422-4424
TAA          4404-4406
TGA          4422-4424
TAG          4404-4406
TGA          4422-4424
TAG          4419-4421
TGA          4422-4424
TGA          4437-4439
TGA          4422-4424
TGA          4437-4439
TAG          4500-4502
TGA          4437-4439
TAG          4500-4502
TGA          4440-4442
TAG          4500-4502
TAA          4449-4451
TAG          4500-4502
TGA          4455-4457
TAG          4500-4502
TAG          4461-4463
TAG          4500-4502
TGA          4470-4472
TAG          4500-4502
TAA          4473-4475
TAG          4500-4502
TAA          4494-4496
TAG          4500-4502
TAG          4497-4499
TAG          4500-4502
TGA          4503-4505
TAG          4500-4502
TGA          4503-4505
TGA          4542-4544
TGA          4503-4505
TGA          4542-4544
TAA          4506-4508
TGA          4542-4544
TGA          4506-4508
TGA          4542-4544
TAA          4506-4508
TGA          4542-4544
TAG          4506-4508
TGA          4542-4544
TAG          4515-4517
TGA          4542-4544
TAA          4521-4523
TGA          4542-4544
TGA          4527-4529
TGA          4542-4544
TGA          4551-4553
TGA          4542-4544
TGA          4551-4553
TAA          4545-4547
TGA          4551-4553
TAG          4566-4568
TGA          4551-4553
TAG          4566-4568
TGA          4563-4565
TAG          4566-4568
TAA          4578-4580
TAG          4566-4568
TAA          4578-4580
TAG          4575-4577
TAA          4578-4580
TAA          4584-4586
TAA          4578-4580
TAA          4584-4586
TAA          4581-4583
TAA          4584-4586
TAA          4617-4619
TAA          4584-4586
TAA          4617-4619
TGA          4587-4589
TAA          4617-4619
TGA          4599-4601
TAA          4617-4619
TAG          4602-4604
TAA          4617-4619
TGA          4635-4637
TAA          4617-4619
TGA          4635-4637
TAG          4620-4622
TGA          4635-4637
TGA          4620-4622
TGA          4635-4637
TGA          4632-4634
TGA          4635-4637
TAG          4638-4640
TGA          4635-4637
TAG          4638-4640
TAG          4677-4679
TAG          4638-4640
TAG          4677-4679
TAG          4641-4643
TAG          4677-4679
TGA          4641-4643
TAG          4677-4679
TAA          4644-4646
TAG          4677-4679
TGA          4644-4646
TAG          4677-4679
TAA          4644-4646
TAG          4677-4679
TAG          4644-4646
TAG          4677-4679
TAG          4653-4655
TAG          4677-4679
TAG          4656-4658
TAG          4677-4679
TGA          4656-4658
TAG          4677-4679
TAG          4665-4667
TAG          4677-4679
TAA          4674-4676
TAG          4677-4679
TAA          4707-4709
TAG          4677-4679
TAA          4707-4709
TAA          4680-4682
TAA          4707-4709
TAA          4683-4685
TAA          4707-4709
TGA          4683-4685
TAA          4707-4709
TAA          4683-4685
TAA          4707-4709
TAG          4683-4685
TAA          4707-4709
TGA          4695-4697
TAA          4707-4709
TGA          4698-4700
TAA          4707-4709
TGA          4716-4718
TAA          4707-4709
TGA          4716-4718
TGA          4836-4838
TGA          4716-4718
TGA          4836-4838
TGA          4722-4724
TGA          4836-4838
TGA          4725-4727
TGA          4836-4838
TGA          4737-4739
TGA          4836-4838
TGA          4740-4742
TGA          4836-4838
TGA          4755-4757
TGA          4836-4838
TGA          4764-4766
TGA          4836-4838
TAA          4773-4775
TGA          4836-4838
TGA          4773-4775
TGA          4836-4838
TAA          4773-4775
TGA          4836-4838
TAG          4773-4775
TGA          4836-4838
TAG          4782-4784
TGA          4836-4838
TGA          4788-4790
TGA          4836-4838
TGA          4794-4796
TGA          4836-4838
TAA          4806-4808
TGA          4836-4838
TGA          4806-4808
TGA          4836-4838
TAA          4806-4808
TGA          4836-4838
TAG          4806-4808
TGA          4836-4838
TGA          4812-4814
TGA          4836-4838
TAG          4824-4826
TGA          4836-4838
TGA          4839-4841
TGA          4836-4838
TGA          4839-4841
TGA          4845-4847
TGA          4839-4841
TGA          4845-4847
TGA          4851-4853
TGA          4845-4847
TGA          4851-4853
TGA          4860-4862
TGA          4851-4853
TGA          4860-4862
TGA          4980-4982
TGA          4860-4862
TGA          4980-4982
TGA          4863-4865
TGA          4980-4982
TAG          4866-4868
TGA          4980-4982
TGA          4875-4877
TGA          4980-4982
TAA          4893-4895
TGA          4980-4982
TAA          4920-4922
TGA          4980-4982
TAA          4935-4937
TGA          4980-4982
TGA          4944-4946
TGA          4980-4982
TAA          4995-4997
TGA          4980-4982
TAA          4995-4997
TAA          4992-4994
TAA          4995-4997
TGA          4992-4994
TAA          4995-4997
TAA          4992-4994
TAA          4995-4997
TAG          4992-4994
TAA          4995-4997
TAA          5151-5153
TAA          4995-4997
TAA          5151-5153
TGA          5004-5006
TAA          5151-5153
TGA          5007-5009
TAA          5151-5153
TAG          5010-5012
TAA          5151-5153
TAG          5016-5018
TAA          5151-5153
TAG          5019-5021
TAA          5151-5153
TGA          5022-5024
TAA          5151-5153
TAA          5025-5027
TAA          5151-5153
TGA          5031-5033
TAA          5151-5153
TGA          5049-5051
TAA          5151-5153
TAG          5052-5054
TAA          5151-5153
TAA          5058-5060
TAA          5151-5153
TAA          5061-5063
TAA          5151-5153
TAG          5064-5066
TAA          5151-5153
TGA          5097-5099
TAA          5151-5153
TGA          5100-5102
TAA          5151-5153
TAA          5115-5117
TAA          5151-5153
TGA          5115-5117
TAA          5151-5153
TAA          5115-5117
TAA          5151-5153
TAG          5115-5117
TAA          5151-5153
TAA          5118-5120
TAA          5151-5153
TAG          5127-5129
TAA          5151-5153
TAA          5130-5132
TAA          5151-5153
TGA          5163-5165
TAA          5151-5153
TGA          5163-5165
TGA          5199-5201
TGA          5163-5165
TGA          5199-5201
TGA          5166-5168
TGA          5199-5201
TAA          5187-5189
TGA          5199-5201
TGA          5193-5195
TGA          5199-5201
TGA          5211-5213
TGA          5199-5201
TGA          5211-5213
TAG          5208-5210
TGA          5211-5213
TGA          5208-5210
TGA          5211-5213
TAG          5262-5264
TGA          5211-5213
TAG          5262-5264
TAA          5223-5225
TAG          5262-5264
TAA          5226-5228
TAG          5262-5264
TGA          5226-5228
TAG          5262-5264
TAA          5226-5228
TAG          5262-5264
TAG          5226-5228
TAG          5262-5264
TAA          5250-5252
TAG          5262-5264
TAG          5253-5255
TAG          5262-5264
TGA          5253-5255
TAG          5262-5264
TAA          5289-5291
TAG          5262-5264
TAA          5289-5291
TAA          5265-5267
TAA          5289-5291
TGA          5265-5267
TAA          5289-5291
TAA          5265-5267
TAA          5289-5291
TAG          5265-5267
TAA          5289-5291
TAG          5271-5273
TAA          5289-5291
TGA          5271-5273
TAA          5289-5291
TGA          5310-5312
TAA          5289-5291
TGA          5310-5312
TGA          5292-5294
TGA          5310-5312
TAG          5295-5297
TGA          5310-5312
TAA          5298-5300
TGA          5310-5312
TAA          5307-5309
TGA          5310-5312
TGA          5316-5318
TGA          5310-5312
TGA          5316-5318
TGA          5322-5324
TGA          5316-5318
TGA          5322-5324
TGA          5604-5606
TGA          5322-5324
TGA          5604-5606
TAG          5328-5330
TGA          5604-5606
TGA          5334-5336
TGA          5604-5606
TAA          5343-5345
TGA          5604-5606
TAG          5349-5351
TGA          5604-5606
TAA          5352-5354
TGA          5604-5606
TAA          5367-5369
TGA          5604-5606
TAG          5376-5378
TGA          5604-5606
TGA          5379-5381
TGA          5604-5606
TGA          5388-5390
TGA          5604-5606
TAG          5391-5393
TGA          5604-5606
TAA          5394-5396
TGA          5604-5606
TAG          5403-5405
TGA          5604-5606
TGA          5412-5414
TGA          5604-5606
TAG          5418-5420
TGA          5604-5606
TGA          5418-5420
TGA          5604-5606
TAG          5421-5423
TGA          5604-5606
TGA          5421-5423
TGA          5604-5606
TAA          5424-5426
TGA          5604-5606
TGA          5424-5426
TGA          5604-5606
TAA          5424-5426
TGA          5604-5606
TAG          5424-5426
TGA          5604-5606
TAA          5439-5441
TGA          5604-5606
TGA          5451-5453
TGA          5604-5606
TGA          5460-5462
TGA          5604-5606
TAG          5463-5465
TGA          5604-5606
TGA          5463-5465
TGA          5604-5606
TAG          5478-5480
TGA          5604-5606
TGA          5478-5480
TGA          5604-5606
TAA          5496-5498
TGA          5604-5606
TGA          5499-5501
TGA          5604-5606
TGA          5556-5558
TGA          5604-5606
TAG          5562-5564
TGA          5604-5606
TGA          5562-5564
TGA          5604-5606
TGA          5568-5570
TGA          5604-5606
TGA          5571-5573
TGA          5604-5606
TAA          5574-5576
TGA          5604-5606
TAG          5601-5603
TGA          5604-5606
TGA          5601-5603
TGA          5604-5606
TGA          5625-5627
TGA          5628-5630
TGA          5637-5639
TAG          5640-5642
TAG          5652-5654
TGA          5658-5660
TGA          5664-5666
TGA          5670-5672
TAG          5676-5678
TAG          5700-5702
TAG          5706-5708

[0046] Two base insertions of the present invention form stop codons as indicated in the following TABLE 7. The formed in-frame stop codon and the location are provided. Any expressed protein from BRCA1 genes with these types of mutations should be truncated accordingly. It should be recognized that the present invention includes insertions of 3n+2 bases, where n is an integer greater than zero and less than 1861. These larger insertions mutations have stop codons at nucleotide numbers corresponding to the listed stop codon at the nucleotide number listed. The corresponding nucleotide numbers of the stop codons will be 3n nucleotides larger than those listed.

TABLE 7
Two Base Insertions
Codon Formed Nucleotide Number
TAG          123-125
TAA          126-128
TAG          126-128
TGA          126-128
TAA          129-131
TAG          129-131
TGA          129-131
TAG          132-134
TAG          141-143
TAG          144-146
TAG          147-149
TAG          150-152
TAA          156-158
TAG          159-161
TAA          162-164
TAA          165-167
TAG          168-170
TAA          171-173
TAA          177-179
TGA          177-179
TAA          180-182
TAA          183-185
TAG          183-185
TGA          183-185
TAG          186-188
TAA          189-191
TAG          189-191
TGA          189-191
TAA          195-197
TAA          198-200
TAG          198-200
TGA          198-200
TAG          204-206
TAA          207-209
TAG          207-209
TGA          207-209
TAA          210-212
TGA          210-212
TAA          213-215
TAG          216-218
TAG          222-224
TAA          225-227
TAG          225-227
TGA          225-227
TAA          228-230
TAA          231-233
TAA          234-236
TAG          234-236
TGA          234-236
TAG          237-239
TAA          243-245
TAA          246-248
TAG          246-248
TGA          246-248
TAA          249-251
TAG          249-251
TGA          249-251
TAA          252-254
TAA          255-257
TAG          255-257
TGA          255-257
TAA          258-260
TAG          258-260
TGA          258-260
TAA          261-263
TAA          267-269
TGA          267-269
TAA          276-278
TAA          282-284
TGA          282-284
TAA          285-287
TGA          285-287
TAG          288-290
TAA          294-296
TAG          294-296
TGA          294-296
TAA          300-302
TAG          300-302
TGA          300-302
TAA          306-308
TAG          306-308
TGA          306-308
TAA          309-311
TAG          309-311
TGA          309-311
TAA          312-314
TAA          315-317
TGA          315-317
TAG          318-320
TAA          321-323
TAA          324-326
TAA          327-329
TAA          330-332
TAA          333-335
TGA          333-335
TAG          342-344
TAA          345-347
TAA          348-350
TAA          351-353
TGA          351-353
TAA          354-356
TAG          354-356
TGA          354-356
TAA          357-359
TAG          366-368
TAG          369-371
TAG          372-374
TAA          378-380
TAG          378-380
TGA          378-380
TAA          381-383
TGA          381-383
TAA          384-386
TAA          387-389
TAA          390-392
TAG          390-392
TGA          390-392
TAG          393-395
TAA          396-398
TAG          396-398
TGA          396-398
TAG          405-407
TAA          408-410
TAG          411-413
TAA          414-416
TAG          414-416
TGA          414-416
TAG          417-419
TAA          420-422
TAG          420-422
TGA          420-422
TAG          423-425
TAA          426-428
TAA          429-431
TAA          432-434
TAG          432-434
TGA          432-434
TAA          435-437
TAA          438-440
TAG          438-440
TGA          438-440
TAG          441-443
TAA          444-446
TAA          447-449
TAG          450-452
TAA          453-455
TAA          456-458
TAA          459-461
TAG          459-461
TGA          459-461
TAG          465-467
TAA          474-476
TAG          477-479
TAG          480-482
TAG          483-485
TAA          486-488
TAG          486-488
TGA          486-488
TAA          489-491
TAA          492-494
TAA          498-500
TAA          501-503
TAG          504-506
TAA          507-509
TAG          507-509
TGA          507-509
TAA          510-512
TAA          513-515
TAG          519-521
TAA          522-524
TAA          525-527
TAA          537-539
TGA          537-539
TAG          540-542
TAG          546-548
TAA          549-551
TAA          555-557
TAG          555-557
TGA          555-557
TAA          558-560
TAG          558-560
TGA          558-560
TAG          564-566
TAA          567-569
TAA          570-572
TAA          576-578
TAG          579-581
TAA          588-590
TAG          588-590
TGA          588-590
TAA          591-593
TAG          597-599
TAA          600-602
TAG          603-605
TAA          606-608
TGA          606-608
TAA          609-611
TAA          615-617
TGA          615-617
TAA          618-620
TGA          618-620
TAA          621-623
TAA          630-632
TGA          630-632
TAA          642-644
TAA          645-647
TGA          645-647
TAA          648-650
TAG          648-650
TGA          648-650
TAG          651-653
TAA          654-656
TAG          654-656
TGA          654-656
TAA          657-659
TAG          660-662
TAA          663-665
TAG          663-665
TGA          663-665
TAG          666-668
TAA          669-671
TAG          669-671
TGA          669-671
TAG          672-674
TAA          675-677
TAG          675-677
TGA          675-677
TAA          678-680
TAG          678-680
TGA          678-680
TAG          681-683
TAG          684-686
TAA          687-689
TAG          690-692
TAA          693-695
TAA          696-698
TAG          699-701
TAA          702-704
TAA          705-707
TAG          705-707
TGA          705-707
TAA          708-710
TAG          708-710
TGA          708-710
TAA          711-713
TAG          714-716
TAG          717-719
TAG          720-722
TAG          726-728
TAA          729-731
TAG          729-731
TGA          729-731
TAA          732-734
TAG          732-734
TGA          732-734
TAA          738-740
TAA          741-743
TAG          750-752
TAA          753-755
TAA          756-758
TAG          759-761
TAG          762-764
TAA          765-767
TAA          768-770
TAA          771-773
TAG          771-773
TGA          771-773
TAG          774-776
TAA          777-779
TAG          777-779
TGA          777-779
TAG          780-782
TAA          783-785
TAA          786-788
TAG          789-791
TAG          792-794
TAA          795-797
TAG          795-797
TGA          795-797
TAG          798-800
TAA          801-803
TAG          801-803
TGA          801-803
TAA          804-806
TAG          804-806
TGA          804-806
TAG          807-809
TAA          810-812
TGA          810-812
TAG          813-815
TAG          816-818
TAA          819-821
TAA          822-824
TAA          825-827
TAG          828-830
TAA          843-845
TAA          846-848
TAA          849-851
TAG          852-854
TAA          855-857
TAG          855-857
TGA          855-857
TAA          858-860
TGA          858-860
TAA          861-863
TAA          864-866
TAG          867-869
TAA          870-872
TGA          870-872
TAG          876-878
TAG          879-881
TAG          882-884
TAA          885-887
TGA          885-887
TAG          894-896
TAA          897-899
TAA          900-902
TAG          900-902
TGA          900-902
TAG          906-908
TAA          909-911
TAA          912-914
TAG          912-914
TGA          912-914
TAG          915-917
TAA          918-920
TAG          918-920
TGA          918-920
TAA          921-923
TAA          924-926
TAG          924-926
TGA          924-926
TAG          930-932
TAG          933-935
TAA          939-941
TAG          939-941
TGA          939-941
TAG          942-944
TAA          945-947
TAA          948-950
TAA          951-953
TAG          957-959
TAA          960-962
TAA          963-965
TAG          963-965
TGA          963-965
TAA          966-968
TAG          966-968
TGA          966-968
TAG          975-977
TAA          978-980
TGA          978-980
TAA          981-983
TAA          984-986
TAA          987-989
TAG          987-989
TGA          987-989
TAA          990-992
TAG          990-992
TGA          990-992
TAA          996-998
TAA          999-1001
TAG          1002-1004
TAA          1005-1007
TAA          1008-1010
TAA          1011-1013
TGA          1011-1013
TAG          1014-1016
TAG          1017-1019
TAA          1020-1022
TAG          1023-1025
TAG          1026-1028
TAA          1029-1031
TAG          1029-1031
TGA          1029-1031
TAA          1032-1034
TAG          1032-1034
TGA          1032-1034
TAA          1035-1037
TAA          1038-1040
TAA          1041-1043
TAA          1044-1046
TAG          1053-1055
TAA          1056-1058
TAG          1056-1058
TGA          1056-1058
TAG          1059-1061
TAA          1062-1064
TAA          1065-1067
TGA          1065-1067
TAA          1074-1076
TAA          1077-1079
TAA          1080-1082
TAG          1080-1082
TGA          1080-1082
TAG          1083-1085
TAG          1086-1088
TAA          1089-1091
TAA          1092-1094
TAG          1095-1097
TAA          1098-1100
TAA          1101-1103
TAG          1101-1103
TGA          1101-1103
TAA          1104-1106
TAG          1107-1109
TAA          1110-1112
TAA          1116-1118
TGA          1116-1118
TAA          1122-1124
TAA          1125-1127
TAG          1128-1130
TAA          1131-1133
TAA          1134-1136
TAG          1137-1139
TAG          1140-1142
TAA          1146-1148
TGA          1146-1148
TAG          1149-1151
TAG          1152-1154
TAA          1161-1163
TAG          1161-1163
TGA          1161-1163
TAG          1164-1166
TAA          1167-1169
TGA          1167-1169
TAA          1170-1172
TAG          1173-1175
TAA          1176-1178
TAG          1176-1178
TGA          1176-1178
TAA          1179-1181
TGA          1179-1181
TAA          1182-1184
TAA          1188-1190
TGA          1188-1190
TAA          1197-1199
TAG          1197-1199
TGA          1197-1199
TAA          1200-1202
TAG          1200-1202
TGA          1200-1202
TAG          1203-1205
TAA          1206-1208
TGA          1206-1208
TAA          1212-1214
TAG          1215-1217
TAA          1218-1220
TAG          1221-1223
TAG          1224-1226
TAG          1227-1229
TAA          1233-1235
TAG          1233-1235
TGA          1233-1235
TAA          1236-1238
TGA          1236-1238
TAA          1239-1241
TAA          1245-1247
TAA          1248-1250
TAA          1251-1253
TAA          1254-1256
TAA          1260-1262
TGA          1260-1262
TAG          1263-1265
TAA          1266-1268
TAG          1269-1271
TAA          1272-1274
TAG          1272-1274
TGA          1272-1274
TAA          1275-1277
TAG          1275-1277
TGA          1275-1277
TAA          1278-1280
TAG          1278-1280
TGA          1278-1280
TAA          1281-1283
TAA          1284-1286
TAG          1287-1289
TAG          1290-1292
TAA          1296-1298
TAG          1296-1298
TGA          1296-1298
TAG          1299-1301
TAA          1302-1304
TAG          1302-1304
TGA          1302-1304
TAG          1305-1307
TAG          1308-1310
TAA          1311-1313
TAG          1311-1313
TGA          1311-1313
TAG          1317-1319
TAG          1320-1322
TAG          1323-1325
TAA          1326-1328
TAG          1326-1328
TGA          1326-1328
TAG          1329-1331
TAA          1332-1334
TAG          1332-1334
TGA          1332-1334
TAA          1335-1337
TAG          1338-1340
TAA          1341-1343
TAG          1344-1346
TAG          1347-1349
TAG          1350-1352
TAG          1353-1355
TAA          1356-1358
TAG          1356-1358
TGA          1356-1358
TAG          1359-1361
TAG          1362-1364
TAA          1368-1370
TAG          1371-1373
TAG          1374-1376
TAG          1377-1379
TAG          1380-1382
TAA          1383-1385
TAG          1383-1385
TGA          1383-1385
TAA          1386-1388
TAG          1386-1388
TGA          1386-1388
TAG          1389-1391
TAA          1392-1394
TAG          1392-1394
TGA          1392-1394
TAA          1395-1397
TAG          1395-1397
TGA          1395-1397
TAG          1398-1400
TAA          1401-1403
TGA          1401-1403
TAA          1404-1406
TAG          1407-1409
TAA          1410-1412
TAG          1410-1412
TGA          1410-1412
TAG          1416-1418
TAA          1419-1421
TAG          1422-1424
TAG          1431-1433
TAG          1434-1436
TAA          1437-1439
TAG          1437-1439
TGA          1437-1439
TAA          1440-1442
TAA          1443-1445
TAG          1443-1445
TGA          1443-1445
TAA          1446-1448
TAA          1449-1451
TAG          1452-1454
TAA          1455-1457
TAG          1458-1460
TAA          1464-1466
TAG          1464-1466
TGA          1464-1466
TAA          1467-1469
TAA          1470-1472
TAG          1470-1472
TGA          1470-1472
TAG          1473-1475
TAG          1476-1478
TAA          1479-1481
TGA          1479-1481
TAA          1482-1484
TAA          1485-1487
TAG          1488-1490
TAG          1491-1493
TAA          1494-1496
TAA          1497-1499
TAA          1500-1502
TAG          1500-1502
TGA          1500-1502
TAG          1503-1505
TAA          1506-1508
TGA          1506-1508
TAA          1509-1511
TAA          1512-1514
TAG          1512-1514
TGA          1512-1514
TAA          1518-1520
TGA          1518-1520
TAA          1521-1523
TGA          1521-1523
TAG          1524-1526
TAA          1527-1529
TAA          1536-1538
TAA          1539-1541
TAG          1539-1541
TGA          1539-1541
TAA          1542-1544
TAG          1548-1550
TAA          1551-1553
TAG          1554-1556
TAA          1557-1559
TAA          1563-1565
TAA          1566-1568
TAG          1569-1571
TAG          1572-1574
TAA          1575-1577
TAG          1575-1577
TGA          1575-1577
TAG          1578-1580
TAA          1581-1583
TAG          1584-1586
TAA          1593-1595
TGA          1593-1595
TAA          1596-1598
TAG          1602-1604
TAA          1614-1616
TAA          1617-1619
TAA          1620-1622
TAA          1623-1625
TAG          1623-1625
TGA          1623-1625
TAA          1626-1628
TAA          1632-1634
TAA          1635-1637
TAA          1638-1640
TGA          1638-1640
TAA          1644-1646
TAA          1647-1649
TAG          1647-1649
TGA          1647-1649
TAG          1650-1652
TAG          1662-1664
TAG          1665-1667
TAA          1668-1670
TAG          1668-1670
TGA          1668-1670
TAA          1671-1673
TAA          1674-1676
TAA          1677-1679
TGA          1677-1679
TAG          1680-1682
TAG          1683-1685
TAA          1686-1688
TAG          1686-1688
TGA          1686-1688
TAG          1689-1691
TAG          1692-1694
TAA          1698-1700
TAA          1701-1703
TGA          1701-1703
TAG          1707-1709
TAA          1710-1712
TAA          1713-1715
TGA          1713-1715
TAA          1716-1718
TAG          1722-1724
TAA          1725-1727
TAA          1728-1730
TAA          1734-1736
TAG          1737-1739
TAA          1743-1745
TGA          1743-1745
TAG          1746-1748
TAG          1752-1754
TAA          1755-1757
TGA          1755-1757
TAA          1758-1760
TGA          1758-1760
TAA          1761-1763
TAA          1764-1766
TAA          1767-1769
TAA          1770-1772
TAG          1773-1775
TAG          1779-1781
TAA          1782-1784
TGA          1782-1784
TAA          1785-1787
TAA          1788-1790
TAA          1791-1793
TAG          1794-1796
TAG          1797-1799
TAA          1800-1802
TAG          1800-1802
TGA          1800-1802
TAA          1803-1805
TAA          1809-1811
TGA          1809-1811
TAG          1812-1814
TAA          1815-1817
TGA          1815-1817
TAA          1818-1820
TAA          1824-1826
TAA          1830-1832
TAG          1833-1835
TAA          1836-1838
TAG          1836-1838
TGA          1836-1838
TAG          1842-1844
TAA          1845-1847
TAG          1848-1850
TAA          1851-1853
TAG          1851-1853
TGA          1851-1853
TAG          1854-1856
TAA          1857-1859
TAG          1857-1859
TGA          1857-1859
TAA          1860-1862
TAA          1863-1865
TAA          1866-1868
TGA          1866-1868
TAG          1869-1871
TAG          1872-1874
TAA          1878-1880
TAA          1881-1883
TAA          1884-1886
TAA          1887-1889
TAA          1890-1892
TAA          1893-1895
TAA          1896-1898
TAA          1899-1901
TAG          1902-1904
TAG          1908-1910
TAA          1911-1913
TAG          1911-1913
TGA          1911-1913
TAA          1914-1916
TAA          1917-1919
TAA          1923-1925
TAA          1926-1928
TAG          1926-1928
TGA          1926-1928
TAA          1929-1931
TAG          1932-1934
TAA          1938-1940
TAA          1941-1943
TAA          1944-1946
TGA          1944-1946
TAA          1947-1949
TGA          1953-1955
TAA          1953-1955
TGA          1953-1955
TAA          1956-1958
TGA          1956-1958
TAA          1959-1961
TGA          1959-1961
TAA          1962-1964
TAG          1962-1964
TGA          1962-1964
TAA          1965-1967
TAG          1965-1967
TGA          1965-1967
TAA          1968-1970
TAA          1971-1973
TAA          1977-1979
TAG          1983-1985
TAG          1989-1991
TAG          1995-1997
TAG          1998-2000
TAA          2001-2003
TAA          2004-2006
TAA          2007-2009
TAA          2013-2015
TAA          2022-2024
TAA          2025-2027
TAG          2025-2027
TGA          2025-2027
TAA          2028-2030
TAG          2031-2033
TAA          2034-2036
TAG          2034-2036
TGA          2034-2036
TAA          2040-2042
TAG          2043-2045
TAA          2046-2048
TAA          2049-2051
TAG          2049-2051
TGA          2049-2051
TAA          2052-2054
TAG          2052-2054
TGA          2052-2054
TAA          2055-2057
TAA          2058-2060
TAG          2061-2063
TAG          2064-2066
TAA          2067-2069
TGA          2067-2069
TAA          2070-2072
TAA          2073-2075
TGA          2073-2075
TAA          2076-2078
TAA          2079-2081
TAA          2082-2084
TAG          2082-2084
TGA          2082-2084
TAA          2085-2087
TAA          2091-2093
TAG          2097-2099
TAA          2100-2102
TAA          2106-2108
TAA          2109-2111
TAA          2112-2114
TAA          2124-2126
TAG          2127-2129
TAG          2130-2132
TAA          2133-2135
TAG          2136-2138
TAG          2142-2144
TAA          2145-2147
TAG          2148-2150
TAG          2151-2153
TAA          2154-2156
TAA          2157-2159
TGA          2157-2159
TAA          2160-2162
TGA          2160-2162
TAA          2163-2165
TAA          2166-2168
TAA          2172-2174
TAG          2175-2177
TAA          2181-2183
TGA          2181-2183
TAA          2184-2186
TAA          2187-2189
TAA          2190-2192
TAG          2196-2198
TAA          2199-2201
TAG          2202-2204
TAA          2205-2207
TAA          2208-2210
TAG          2208-2210
TGA          2208-2210
TAG          2214-2216
TAA          2220-2222
TGA          2220-2222
TAA          2223-2225
TAG          2223-2225
TGA          2223-2225
TAA          2226-2228
TAA          2229-2231
TAG          2232-2234
TAG          2238-2240
TAA          2241-2243
TAG          2241-2243
TGA          2241-2243
TAA          2244-2246
TAG          2244-2246
TGA          2244-2246
TAA          2247-2249
TAA          2250-2252
TAA          2253-2255
TAG          2253-2255
TGA          2253-2255
TAA          2256-2258
TAG          2256-2258
TGA          2256-2258
TAA          2259-2261
TAA          2262-2264
TAA          2265-2267
TAG          2268-2270
TAA          2274-2276
TAG          2277-2279
TAA          2280-2282
TAG          2280-2282
TGA          2280-2282
TAG          2283-2285
TAA          2286-2288
TAA          2292-2294
TAA          2301-2303
TAG          2304-2306
TAG          2307-2309
TAA          2310-2312
TAG          2313-2315
TAG          2316-2318
TAA          2319-2321
TGA          2319-2321
TAG          2325-2327
TAA          2328-2330
TAG          2331-2333
TAA          2334-2336
TAG          2337-2339
TAA          2340-2342
TAG          2340-2342
TGA          2340-2342
TAA          2343-2345
TAA          2346-2348
TAG          2349-2351
TAG          2352-2354
TAG          2355-2357
TAA          2361-2363
TAG          2364-2366
TAA          2370-2372
TAA          2373-2375
TAG          2373-2375
TGA          2373-2375
TAA          2376-2378
TAG          2379-2381
TAG          2382-2384
TAA          2385-2387
TAG          2388-2390
TAA          2391-2393
TAG          2391-2393
TGA          2391-2393
TAA          2397-2399
TAG          2400-2402
TAA          2403-2405
TAA          2406-2408
TAG          2406-2408
TGA          2406-2408
TAG          2409-2411
TAG          2412-2414
TAA          2415-2417
TGA          2415-2417
TAA          2418-2420
TAA          2421-2423
TAA          2424-2426
TAA          2427-2429
TAG          2427-2429
TGA          2427-2429
TAG          2433-2435
TAG          2439-2441
TAA          2442-2444
TAG          2445-2447
TAA          2448-2450
TAG          2448-2450
TGA          2448-2450
TAG          2451-2453
TAA          2454-2456
TAG          2460-2462
TAA          2463-2465
TAA          2466-2468
TAA          2469-2471
TAG          2469-2471
TGA          2469-2471
TAA          2472-2474
TAG          2472-2474
TGA          2472-2474
TAG          2478-2480
TAG          2481-2483
TAA          2484-2486
TAA          2487-2489
TAG          2493-2495
TAA          2496-2498
TGA          2496-2498
TAG          2499-2501
TAA          2502-2504
TAA          2505-2507
TAG          2508-2510
TAA          2514-2516
TAA          2517-2519
TAA          2520-2522
TAG          2520-2522
TGA          2520-2522
TAG          2523-2525
TAA          2526-2528
TGA          2526-2528
TAA          2532-2534
TAG          2532-2534
TGA          2532-2534
TAG          2535-2537
TAG          2538-2540
TAA          2541-2543
TAG          2541-2543
TGA          2541-2543
TAG          2544-2546
TAA          2547-2549
TAA          2553-2555
TAG          2556-2558
TAA          2562-2564
TAG          2568-2570
TAA          2571-2573
TAG          2571-2573
TGA          2571-2573
TAA          2574-2576
TAG          2574-2576
TGA          2574-2576
TAA          2577-2579
TAG          2580-2582
TAA          2583-2585
TAA          2586-2588
TAA          2589-2591
TAG          2592-2594
TAA          2595-2597
TAG          2598-2600
TAG          2601-2603
TAA          2604-2606
TAG          2604-2606
TGA          2604-2606
TAA          2607-2609
TAA          2610-2612
TAG          2610-2612
TGA          2610-2612
TAA          2616-2618
TAG          2616-2618
TGA          2616-2618
TAG          2619-2621
TAG          2625-2627
TAG          2628-2630
TAA          2631-2633
TAA          2637-2639
TAG          2643-2645
TAA          2646-2648
TAA          2649-2651
TAA          2652-2654
TAG          2655-2657
TAA          2658-2660
TAG          2661-2663
TAG          2664-2666
TAA          2667-2669
TAG          2670-2672
TAG          2676-2678
TAG          2679-2681
TAA          2685-2687
TAG          2685-2687
TGA          2685-2687
TAA          2688-2690
TAG          2688-2690
TGA          2688-2690
TAA          2694-2696
TGA          2694-2696
TAA          2697-2699
TAA          2700-2702
TAG          2700-2702
TGA          2700-2702
TAA          2703-2705
TAG          2706-2708
TAA          2709-2711
TAG          2709-2711
TGA          2709-2711
TAA          2712-2714
TAA          2721-2723
TAG          2721-2723
TGA          2721-2723
TAA          2724-2726
TAG          2724-2726
TGA          2724-2726
TAG          2727-2729
TAA          2733-2735
TAG          2733-2735
TGA          2733-2735
TAA          2736-2738
TAG          2736-2738
TGA          2736-2738
TAA          2739-2741
TAG          2745-2747
TAA          2748-2750
TAG          2751-2753
TAG          2754-2756
TAG          2757-2759
TAG          2760-2762
TAA          2763-2765
TAG          2763-2765
TGA          2763-2765
TAG          2766-2768
TAA          2769-2771
TAA          2772-2774
TAG          2772-2774
TGA          2772-2774
TAA          2775-2777
TAG          2775-2777
TGA          2775-2777
TAG          2778-2780
TAA          2784-2786
TAG          2784-2786
TGA          2784-2786
TAG          2787-2789
TAA          2790-2792
TAG          2790-2792
TGA          2790-2792
TAA          2793-2795
TAG          2793-2795
TGA          2793-2795
TAA          2796-2798
TAA          2799-2801
TGA          2799-2801
TAA          2805-2807
TAA          2811-2813
TAG          2814-2816
TAA          2817-2819
TAA          2820-2822
TAG          2820-2822
TGA          2820-2822
TAG          2823-2825
TAA          2826-2828
TAG          2826-2828
TGA          2826-2828
TAG          2829-2831
TAA          2835-2837
TAG          2838-2840
TAG          2841-2843
TAA          2844-2846
TAG          2850-2852
TAA          2853-2855
TAA          2856-2858
TGA          2856-2858
TAG          2859-2861
TAA          2862-2864
TAG          2862-2864
TGA          2862-2864
TAA          2865-2867
TAA          2868-2870
TAA          2871-2873
TAG          2877-2879
TAA          2883-2885
TGA          2883-2885
TAG          2886-2888
TAA          2889-2891
TAA          2892-2894
TAA          2895-2897
TAG          2898-2900
TAG          2901-2903
TAA          2904-2906
TAG          2904-2906
TGA          2904-2906
TAG          2910-2912
TAG          2913-2915
TAG          2916-2918
TAA          2922-2924
TGA          2922-2924
TAG          2925-2927
TAA          2928-2930
TAG          2934-2936
TAG          2937-2939
TAA          2940-2942
TAG          2943-2945
TAA          2946-2948
TAA          2949-2951
TAG          2949-2951
TGA          2949-2951
TAA          2952-2954
TAA          2955-2957
TAA          2958-2960
TAG          2961-2963
TAG          2964-2966
TAA          2967-2969
TAG          2967-2969
TGA          2967-2969
TAA          2970-2972
TAA          2973-2975
TAG          2973-2975
TGA          2973-2975
TAA          2976-2978
TAG          2976-2978
TGA          2976-2978
TAA          2982-2984
TAG          2982-2984
TGA          2982-2984
TAA          2985-2987
TAG          2985-2987
TGA          2985-2987
TAA          2991-2993
TAG          2991-2993
TGA          2991-2993
TAA          2994-2996
TAG          2997-2999
TAA          3000-3002
TAG          3003-3005
TAA          3006-3008
TAG          3009-3011
TAA          3015-3017
TAA          3018-3020
TAA          3024-3026
TAA          3027-3029
TAG          3033-3035
TAA          3039-3041
TAG          3039-3041
TGA          3039-3041
TAA          3045-3047
TAA          3051-3053
TAG          3051-3053
TGA          3051-3053
TAA          3057-3059
TAA          3069-3071
TAG          3069-3071
TGA          3069-3071
TAA          3075-3077
TAA          3078-3080
TAA          3081-3083
TAG          3081-3083
TGA          3081-3083
TAA          3084-3086
TAG          3084-3086
TGA          3084-3086
TAG          3087-3089
TAA          3090-3092
TAA          3093-3095
TAA          3096-3098
TAA          3099-3101
TAG          3099-3101
TGA          3099-3101
TAA          3102-3104
TAA          3105-3107
TGA          3105-3107
TAA          3108-3110
TAG          3117-3119
TAG          3120-3122
TAA          3123-3125
TAA          3126-3128
TAG          3126-3128
TGA          3126-3128
TAG          3129-3131
TAG          3132-3134
TAA          3138-3140
TAG          3138-3140
TGA          3138-3140
TAA          3141-3143
TAA          3144-3146
TAG          3144-3146
TGA          3144-3146
TAG          3150-3152
TAA          3153-3155
TAG          3156-3158
TAA          3159-3161
TAG          3162-3164
TAA          3165-3167
TAG          3168-3170
TAA          3171-3173
TGA          3171-3173
TAA          3174-3176
TAA          3180-3182
TAA          3183-3185
TAG          3186-3188
TAA          3189-3191
TGA          3189-3191
TAA          3192-3194
TAA          3195-3197
TAA          3198-3200
TAA          3204-3206
TAA          3207-3209
TAA          3210-3212
TAA          3213-3215
TAG          3216-3218
TAA          3219-3221
TAG          3222-3224
TAA          3225-3227
TAG          3225-3227
TGA          3225-3227
TAA          3228-3230
TAG          3231-3233
TAG          3234-3236
TAA          3237-3239
TAA          3240-3242
TAG          3240-3242
TGA          3240-3242
TAA          3243-3245
TAA          3246-3248
TAA          3249-3251
TAA          3252-3254
TAG          3255-3257
TAG          3258-3260
TAG          3261-3263
TAA          3264-3266
TAG          3264-3266
TGA          3264-3266
TAA          3267-3269
TAA          3270-3272
TAA          3273-3275
TAG          3276-3278
TAG          3279-3281
TAG          3282-3284
TAA          3285-3287
TAG          3285-3287
TGA          3285-3287
TAA          3288-3290
TAA          3291-3293
TAA          3294-3296
TAG          3297-3299
TAA          3300-3302
TAG          3303-3305
TAA          3306-3308
TAG          3306-3308
TGA          3306-3308
TAA          3309-3311
TAG          3312-3314
TAG          3315-3317
TAA          3318-3320
TAA          3321-3323
TAG          3327-3329
TAG          3330-3332
TAG          3336-3338
TAA          3339-3341
TAA          3342-3344
TAA          3345-3347
TAG          3348-3350
TAA          3354-3356
TAA          3357-3359
TAG          3357-3359
TGA          3357-3359
TAA          3360-3362
TGA          3360-3362
TAG          3363-3365
TAA          3366-3368
TAA          3372-3374
TAA          3375-3377
TAG          3375-3377
TGA          3375-3377
TAG          3378-3380
TAG          3381-3383
TAA          3384-3386
TAG          3384-3386
TGA          3384-3386
TAG          3393-3395
TAG          3396-3398
TAA          3399-3401
TAG          3399-3401
TGA          3399-3401
TAA          3402-3404
TAA          3408-3410
TAG          3417-3419
TAA          3420-3422
TAA          3423-3425
TAA          3426-3428
TAG          3426-3428
TGA          3426-3428
TAA          3429-3431
TAG          3438-3440
TAA          3441-3443
TAA          3444-3446
TAA          3447-3449
TAG          3453-3455
TAA          3456-3458
TAG          3456-3458
TGA          3456-3458
TAG          3459-3461
TAG          3462-3464
TAG          3465-3467
TAG          3468-3470
TAA          3474-3476
TGA          3474-3476
TAG          3477-3479
TAA          3480-3482
TAA          3483-3485
TAG          3486-3488
TAA          3489-3491
TAG          3489-3491
TGA          3489-3491
TAA          3492-3494
TAG          3492-3494
TGA          3492-3494
TAA          3498-3500
TAG          3498-3500
TGA          3498-3500
TAA          3504-3506
TGA          3504-3506
TAA          3507-3509
TAG          3507-3509
TGA          3507-3509
TAG          3510-3512
TAA          3513-3515
TAA          3516-3518
TAG          3516-3518
TGA          3516-3518
TAG          3519-3521
TAA          3528-3530
TAG          3531-3533
TAA          3534-3536
TAA          3537-3539
TAG          3543-3545
TAA          3546-3548
TAG          3546-3548
TGA          3546-3548
TAG          3552-3554
TAA          3555-3557
TAG          3555-3557
TGA          3555-3557
TAA          3558-3560
TAG          3558-3560
TGA          3558-3560
TAG          3561-3563
TAA          3564-3566
TGA          3564-3566
TAG          3570-3572
TAG          3573-3575
TAA          3579-3581
TAG          3579-3581
TGA          3579-3581
TAG          3582-3584
TAG          3585-3587
TAG          3588-3590
TAG          3591-3593
TAA          3594-3596
TAA          3597-3599
TAG          3600-3602
TAG          3603-3605
TAA          3606-3608
TAA          3609-3611
TAA          3612-3614
TAG          3612-3614
TGA          3612-3614
TAG          3615-3617
TAG          3618-3620
TAA          3621-3623
TAG          3624-3626
TAA          3627-3629
TAA          3630-3632
TAG          3633-3635
TAA          3636-3638
TAA          3639-3641
TAG          3639-3641
TGA          3639-3641
TAG          3642-3644
TAG          3645-3647
TAA          3648-3650
TAG          3648-3650
TGA          3648-3650
TAA          3651-3653
TAA          3654-3656
TAA          3657-3659
TAG          3660-3662
TAA          3666-3668
TGA          3666-3668
TAG          3669-3671
TAG          3672-3674
TAA          3678-3680
TAA          3681-3683
TAA          3684-3686
TGA          3684-3686
TAA          3690-3692
TAA          3696-3698
TAG          3696-3698
TGA          3696-3698
TAA          3699-3701
TAA          3705-3707
TAA          3711-3713
TAG          3711-3713
TGA          3711-3713
TAG          3714-3716
TAG          3720-3722
TAA          3723-3725
TAG          3723-3725
TGA          3723-3725
TAA          3729-3731
TAG          3732-3734
TAG          3735-3737
TAA          3738-3740
TAA          3741-3743
TGA          3741-3743
TAA          3744-3746
TAG          3744-3746
TGA          3744-3746
TAG          3747-3749
TAA          3750-3752
TAG          3750-3752
TGA          3750-3752
TAA          3753-3755
TAG          3753-3755
TGA          3753-3755
TAG          3756-3758
TAG          3759-3761
TAA          3762-3764
TGA          3762-3764
TAA          3765-3767
TAG          3765-3767
TGA          3765-3767
TAA          3768-3770
TAG          3768-3770
TGA          3768-3770
TAA          3771-3773
TAG          3774-3776
TAG          3777-3779
TAG          3780-3782
TAG          3783-3785
TAA          3792-3794
TAG          3792-3794
TGA          3792-3794
TAA          3795-3797
TAG          3795-3797
TGA          3795-3797
TAA          3804-3806
TAG          3804-3806
TGA          3804-3806
TAA          3807-3809
TAG          3807-3809
TGA          3807-3809
TAA          3810-3812
TAG          3810-3812
TGA          3810-3812
TAG          3813-3815
TAA          3816-3818
TAG          3819-3821
TAA          3822-3824
TAA          3825-3827
TAA          3828-3830
TAA          3834-3836
TAG          3834-3836
TGA          3834-3836
TAA          3840-3842
TAG          3840-3842
TGA          3840-3842
TAA          3843-3845
TAA          3846-3848
TAA          3852-3854
TAA          3855-3857
TAG          3858-3860
TAG          3861-3863
TAA          3864-3866
TAG          3867-3869
TAA          3870-3872
TAG          3870-3872
TGA          3870-3872
TAA          3876-3878
TAG          3876-3878
TGA          3876-3878
TAA          3879-3881
TAA          3882-3884
TGA          3882-3884
TAA          3885-3887
TAG          3888-3890
TAG          3891-3893
TAA          3894-3896
TGA          3894-3896
TAA          3897-3899
TAG          3897-3899
TGA          3897-3899
TAA          3900-3902
TAG          3900-3902
TGA          3900-3902
TAA          3903-3905
TAG          3903-3905
TGA          3903-3905
TAA          3906-3908
TAG          3906-3908
TGA          3906-3908
TAA          3909-3911
TGA          3909-3911
TAA          3912-3914
TGA          3912-3914
TAA          3915-3917
TAA          3918-3920
TAG          3918-3920
TGA          3918-3920
TAA          3921-3923
TAG          3924-3926
TAA          3927-3929
TAG          3927-3929
TGA          3927-3929
TAA          3930-3932
TAA          3933-3935
TAG          3939-3941
TAA          3942-3944
TAA          3945-3947
TAG          3945-3947
TGA          3945-3947
TAG          3948-3950
TAA          3951-3953
TAG          3954-3956
TAA          3957-3959
TAG          3957-3959
TGA          3957-3959
TAG          3963-3965
TAA          3975-3977
TAG          3978-3980
TAG          3981-3983
TAA          3984-3986
TAA          3987-3989
TAA          3990-3992
TAG          3990-3992
TGA          3990-3992
TAA          3993-3995
TAG          3993-3995
TGA          3993-3995
TAG          3996-3998
TAA          3999-4001
TAA          4002-4004
TAG          4002-4004
TGA          4002-4004
TAA          4005-4007
TAG          4005-4007
TGA          4005-4007
TAA          4008-4010
TAG          4008-4010
TGA          4008-4010
TAA          4011-4013
TAG          4011-4013
TGA          4011-4013
TAA          4017-4019
TAG          4017-4019
TGA          4017-4019
TAA          4020-4022
TAG          4023-4025
TAA          4026-4028
TAG          4026-4028
TGA          4026-4028
TAG          4029-4031
TAG          4032-4034
TAA          4035-4037
TAG          4035-4037
TGA          4035-4037
TAA          4038-4040
TGA          4038-4040
TAG          4041-4043
TAA          4044-4046
TAA          4047-4049
TAA          4050-4052
TAA          4053-4055
TAG          4059-4061
TAA          4065-4067
TAG          4065-4067
TGA          4065-4067
TAA          4068-4070
TAG          4068-4070
TGA          4068-4070
TAA          4071-4073
TGA          4071-4073
TAG          4074-4076
TAA          4077-4079
TAG          4077-4079
TGA          4077-4079
TAA          4080-4082
TAG          4080-4082
TGA          4080-4082
TAA          4083-4085
TAA          4089-4091
TAA          4092-4094
TGA          4092-4094
TAA          4101-4103
TAG          4101-4103
TGA          4101-4103
TAG          4104-4106
TAA          4107-4109
TAG          4113-4115
TAG          4116-4118
TAG          4119-4121
TAA          4125-4127
TGA          4125-4127
TAG          4128-4130
TAA          4131-4133
TAG          4134-4136
TAA          4137-4139
TAG          4137-4139
TGA          4137-4139
TAG          4140-4142
TAA          4143-4145
TAG          4143-4145
TGA          4143-4145
TAG          4146-4148
TAG          4149-4151
TAG          4152-4154
TAG          4155-4157
TAA          4158-4160
TAG          4161-4163
TAA          4164-4166
TAG          4167-4169
TAA          4170-4172
TAG          4170-4172
TGA          4170-4172
TAG          4173-4175
TAG          4176-4178
TAA          4179-4181
TAA          4182-4184
TAG          4188-4190
TAG          4191-4193
TAA          4197-4199
TAA          4200-4202
TAG          4203-4205
TAA          4206-4208
TAG          4206-4208
TGA          4206-4208
TAA          4209-4211
TAA          4212-4214
TAG          4212-4214
TGA          4212-4214
TAG          4215-4217
TAG          4218-4220
TAG          4221-4223
TAG          4224-4226
TAA          4227-4229
TAG          4227-4229
TGA          4227-4229
TAG          4230-4232
TAA          4233-4235
TAG          4233-4235
TGA          4233-4235
TAG          4236-4238
TAA          4239-4241
TGA          4239-4241
TAG          4242-4244
TAA          4245-4247
TAA          4248-4250
TAG          4251-4253
TAA          4254-4256
TAG          4254-4256
TGA          4254-4256
TAG          4257-4259
TAG          4260-4262
TAA          4263-4265
TAG          4263-4265
TGA          4263-4265
TAA          4266-4268
TAG          4266-4268
TGA          4266-4268
TAG          4269-4271
TAA          4275-4277
TAG          4275-4277
TGA          4275-4277
TAA          4278-4280
TAG          4278-4280
TGA          4278-4280
TAA          4284-4286
TGA          4284-4286
TAG          4287-4289
TAA          4290-4292
TAA          4293-4295
TAG          4293-4295
TGA          4293-4295
TAA          4296-4298
TAA          4299-4301
TAA          4308-4310
TGA          4308-4310
TAG          4311-4313
TAA          4314-4316
TAA          4317-4319
TAA          4326-4328
TAA          4332-4334
TGA          4332-4334
TAA          4335-4337
TAG          4347-4349
TAA          4350-4352
TAG          4353-4355
TAG          4356-4358
TAG          4362-4364
TAG          4365-4367
TAG          4368-4370
TAA          4371-4373
TAG          4371-4373
TGA          4371-4373
TAG          4374-4376
TAG          4383-4385
TAA          4386-4388
TGA          4386-4388
TAA          4395-4397
TAG          4395-4397
TGA          4395-4397
TAA          4398-4400
TAA          4401-4403
TAA          4404-4406
TAG          4404-4406
TGA          4404-4406
TAA          4410-4412
TAG          4410-4412
TGA          4410-4412
TAA          4413-4415
TAA          4416-4418
TAA          4419-4421
TAG          4422-4424
TAA          4425-4427
TAG          4425-4427
TGA          4425-4427
TAA          4428-4430
TAG          4428-4430
TGA          4428-4430
TAG          4431-4433
TAG          4437-4439
TAG          4440-4442
TAA          4449-4451
TAG          4455-4457
TAA          4461-4463
TAA          4464-4466
TAA          4467-4469
TAG          4467-4469
TGA          4467-4469
TAG          4470-4472
TAA          4473-4475
TAG          4476-4478
TAG          4479-4481
TAA          4482-4484
TAG          4482-4484
TGA          4482-4484
TAA          4485-4487
TAA          4488-4490
TAG          4488-4490
TGA          4488-4490
TAA          4494-4496
TGA          4494-4496
TAA          4497-4499
TAA          4500-4502
TAG          4503-4505
TAA          4506-4508
TAG          4506-4508
TGA          4506-4508
TAA          4512-4514
TAA          4515-4517
TAA          4521-4523
TGA          4521-4523
TAG          4527-4529
TAG          4530-4532
TAA          4536-4538
TAG          4536-4538
TGA          4536-4538
TAG          4539-4541
TAG          4542-4544
TAA          4545-4547
TAA          4548-4550
TAG          4548-4550
TGA          4548-4550
TAG          4551-4553
TAG          4554-4556
TAA          4557-4559
TAG          4557-4559
TGA          4557-4559
TAG          4560-4562
TAG          4563-4565
TAA          4566-4568
TAA          4569-4571
TAG          4569-4571
TGA          4569-4571
TAA          4572-4574
TAA          4575-4577
TAA          4578-4580
TAA          4581-4583
TAA          4584-4586
TAG          4587-4589
TAG          4593-4595
TAG          4596-4598
TAG          4599-4601
TAA          4602-4604
TAA          4605-4607
TAG          4605-4607
TGA          4605-4607
TAA          4608-4610
TAG          4608-4610
TGA          4608-4610
TAA          4614-4616
TAG          4614-4616
TGA          4614-4616
TAA          4617-4619
TAA          4620-4622
TAG          4620-4622
TGA          4620-4622
TAA          4626-4628
TAG          4626-4628
TGA          4626-4628
TAA          4629-4631
TAG          4629-4631
TGA          4629-4631
TAG          4632-4634
TAG          4635-4637
TAA          4638-4640
TAA          4641-4643
TAG          4641-4643
TGA          4641-4643
TAA          4644-4646
TAG          4644-4646
TGA          4644-4646
TAA          4647-4649
TAA          4653-4655
TAA          4656-4658
TAG          4656-4658
TGA          4656-4658
TAA          4659-4661
TAG          4659-4661
TGA          4659-4661
TAG          4662-4664
TAA          4665-4667
TGA          4665-4667
TAA          4674-4676
TGA          4674-4676
TAA          4677-4679
TAA          4680-4682
TAA          4683-4685
TAG          4683-4685
TGA          4683-4685
TAA          4689-4691
TAG          4689-4691
TGA          4689-4691
TAG          4695-4697
TAG          4698-4700
TAA          4704-4706
TAA          4707-4709
TAG          4710-4712
TAG          4713-4715
TAG          4716-4718
TAG          4719-4721
TAG          4722-4724
TAG          4725-4727
TAG          4737-4739
TAG          4740-4742
TAA          4743-4745
TAG          4743-4745
TGA          4743-4745
TAG          4746-4748
TAG          4755-4757
TAA          4758-4760
TAG          4758-4760
TGA          4758-4760
TAA          4761-4763
TGA          4761-4763
TAG          4764-4766
TAA          4767-4769
TAA          4770-4772
TAG          4770-4772
TGA          4770-4772
TAA          4773-4775
TAG          4773-4775
TGA          4773-4775
TAA          4776-4778
TAG          4776-4778
TGA          4776-4778
TAA          4782-4784
TAG          4788-4790
TAG          4794-4796
TAG          4797-4799
TAA          4800-4802
TAA          4806-4808
TAG          4806-4808
TGA          4806-4808
TAG          4812-4814
TAA          4815-4817
TAG          4815-4817
TGA          4815-4817
TAG          4818-4820
TAA          4821-4823
TAA          4824-4826
TAA          4830-4832
TAG          4830-4832
TGA          4830-4832
TAA          4833-4835
TAG          4833-4835
TGA          4833-4835
TAG          4836-4838
TAG          4839-4841
TAG          4845-4847
TAA          4848-4850
TAG          4848-4850
TGA          4848-4850
TAG          4851-4853
TAA          4857-4859
TAG          4857-4859
TGA          4857-4859
TAG          4860-4862
TAG          4863-4865
TAA          4866-4868
TAG          4869-4871
TAG          4875-4877
TAA          4878-4880
TAG          4878-4880
TGA          4878-4880
TAG          4881-4883
TAG          4887-4889
TAG          4890-4892
TAA          4893-4895
TAA          4896-4898
TAA          4902-4904
TAG          4902-4904
TGA          4902-4904
TAA          4905-4907
TAG          4905-4907
TGA          4905-4907
TAA          4908-4910
TAA          4911-4913
TAG          4911-4913
TGA          4911-4913
TAG          4914-4916
TAA          4917-4919
TAG          4917-4919
TGA          4917-4919
TAA          4920-4922
TGA          4920-4922
TAG          4923-4925
TAA          4932-4934
TAG          4932-4934
TGA          4932-4934
TAA          4935-4937
TGA          4935-4937
TAG          4938-4940
TAG          4941-4943
TAG          4944-4946
TAA          4947-4949
TAG          4947-4949
TGA          4947-4949
TAG          4950-4952
TAG          4956-4958
TAG          4962-4964
TAG          4965-4967
TAG          4968-4970
TAA          4974-4976
TAA          4977-4979
TAG          4980-4982
TAA          4983-4985
TAG          4986-4988
TAG          4989-4991
TAA          4992-4994
TAG          4992-4994
TGA          4992-4994
TAA          4995-4997
TAG          4998-5000
TAA          5001-5003
TAG          5004-5006
TAG          5007-5009
TAA          5010-5012
TAG          5013-5015
TAA          5016-5018
TGA          5016-5018
TAA          5019-5021
TAG          5022-5024
TAA          5025-5027
TGA          5025-5027
TAG          5031-5033
TAA          5034-5036
TAG          5034-5036
TGA          5034-5036
TAA          5037-5039
TGA          5037-5039
TAG          5040-5042
TAA          5043-5045
TAG          5043-5045
TGA          5043-5045
TAA          5046-5048
TAG          5049-5051
TAA          5052-5054
TAG          5055-5057
TAA          5058-5060
TAA          5061-5063
TAA          5064-5066
TAA          5067-5069
TAA          5070-5072
TAG          5070-5072
TGA          5070-5072
TAA          5073-5075
TAG          5076-5078
TGA          5091-5093

[0047] Insertion mutant BRCA1 genes containing a truncating mutation may also be defined as having the sequence 5′ R1-R2-R3 3′; where R1 is the wild type BRCA1 DNA sequence from nucleotide number 120 to X; R2 is 3n+1 or 3n+2 nucleotides of any sequence where n is an integer of zero or greater; R3 contains the wild type BRCA1 DNA sequence of nucleotide number X+1 to 5711, and where X=123 to 5707.

[0048] Alternatively, an insertion mutant BRCA1 gene or fragment thereof containing a truncating mutation is capable of specifically hybridizing to an oligonucleotide probe being at least 9 nucleotides in length and having the sequence 5′ R1-R2-R3 3′; where R1 contains at its 3′ end three nucleotides complementary to nucleotide numbers X-2, X-1 and X of the wild-type BRCA1 gene; R2=an oligonucleotide having Y nucleotides of any sequence; R3 contains at its 5′ end three nucleotides complementary to nucleotide numbers X+1, X+2 and X+3 of the wild type BRCA1 gene; where Y is 3n+1 or 3n+2 where n is an integer of zero to 1861, and where X=122 to 5707.

[0049] It should be recognized that for TABLES 4-7, each line indicating a stop codon represents several different mutations, each one of which creates the same truncating stop codon. For example, in TABLE 4, the line indicated by TGA stop codon at 207-209 represents twenty-four different mutations, 185delA, 186delG, 187delA, 188delG, 189delT, 190delG, 191 delT, 192delC, 193delC, 194delC, 195delA, 196delT, 197delC, 198delT, 199delG, 200delT, 201delC, 202delT, 203delG, 204delG, 205delA, 206delG, 207delT, 208delT. The corresponding mutations for each line in each table are easily determined by anyone of very modest skill in the art knowing only the BRCA1 sequence such as given SEQ ID NO:1 and TABLES 4-7.

[0050] A substantially complete listing of all of the mutations, their sites etc. of the present invention is described in Appendix A, B, C, D and E. Likewise, the choice of mutations and corresponding oligonucleotides may be chosen from and determined by the list in Appendix A-E.

[0051] An alternative method for defining the mutations of the present invention is by their nucleotide numbers. Mutant BRCA1 genes having nonsense mutations may be described as having the nucleotide sequence R4-R5-R6, where R4 is nucleotide numbers 120 to 3×of the BRCA1 gene; R5 is TAG, TAA or TGA; and R6 is nucleotide numbers 3X+4 to 5711 of the BRCA1 gene; where X is 41 to 1903.

[0052] Mutant BRCA1 genes having deletion mutations may be described as having the nucleotide sequence R4-R5, where R4 is nucleotide numbers 120 to Y of the BRCA1 gene; and R5 is nucleotide numbers Y+Z+1 to 5711 of the BRCA1 gene; where Y is 124 to 5707, and Z is 3n+1 or 3n+2 where N is an integer of zero or greater.

[0053] Mutant BRCA1 genes having insertion mutations may be described as having the nucleotide sequence R4-R5-R6, where R4 is nucleotide numbers 120 to Y of the BRCA1 gene; R5 is 3n+1 or 3n+2 nucleotides of any sequence where n is an integer of zero or greater; and R6 is nucleotides Y+1 to 5711; wherein Y is from 122 to 5707.

[0054] While the present invention encompasses genes with numerous mutations in the BRCA1 gene, applicant reserves the right to lessen the scope and number of mutations to be included in the present invention.

[0055] It should be recognized that mutations causing truncations which form a smaller protein molecule than mutations causing truncations of known mutations associated with cancer are expected to also be associated with cancer. Removing additional amino acids from a non-function protein is also believed to result in a non-functional protein.

[0056] Useful oligonucleotides according to the present invention are those which will specifically hybridize to BRCA1 sequences in the region of the mutations. The oligonucleotides of the present invention are preferably “biologically active” with respect to structural attributes, such as the capacity of a nucleic acid to hybridize to another nucleic acid molecule or to be used by a polymerase as a primer. Alternatively, such attributes may be catalytic, and thus involve the capacity of the agent to mediate a chemical reaction or response. Typically these oligonucleotides are about 13 to 27 nucleotides in length (longer for large insertions) and have the nucleotide sequence corresponding to the region of the mutations at their respective nucleotide locations on the BRCA1 sequence. Such molecules can be labeled, according to any technique known in the art, such as with radiolabels, fluorescent labels, enzymatic labels, sequence tags, biotin, other ligands, etc.

[0057] According to another aspect of the invention, the oligonucleotides contain one or more of the specific mutations constituting DNA probes. Generally it is preferred for each DNA probe to encompass only one mutation. Such molecules may be labeled and can be used as allele-specific oligonucleotide probes to detect the mutation of interest.

[0058] Alternatively, the oligonucleotide may be one primer of a PCR primer pair, which upon annealing, will amplify a product. In the situation wherein the target DNA sample does not contain a sequence complementary to the oligonucleotide, annealing does not occur, and thus amplification of a product does not occur.

[0059] Polynucleotide-containing biological samples, such as blood, can be tested to determine whether the BRCA1 gene contains one of the specific mutations listed above. To amplify the BRCA1 gene, one may use PCR using primers which hybridize to the ends of the exons or to the introns flanking the exons. To detect mutations in the introns, primers amplifying the introns, especially the regions adjacent to the exons (particularly the splice site regions), may be used. Examples of suitable primers are given in Friedman et al., Nat. Genetics, 8:399-404 (1994).

[0060] Amplification may also be performed by a number of other techniques, such as by cloning the gene or gene fragments, and linking the BRCA1 gene or fragments thereof in the sample to a vector. “Shot gun” cloning is particularly preferred. For the purposes of this to application, a vector may be any polynucleotide containing system which induces replication such as a plasmid, cosmid, virus, transposon, or portions thereof.

[0061] In one embodiment of the invention, the BRCA1 gene or A DNA fragment complementary to its coding sequence is ligated to a vector which is placed inside a suitable host cell or other system for replicating the vector. After replication, the BRCA1 gene or its fragments are then separated from the vector, e.g. by restriction endonuclease digestion, to amplify the copy number of BRCA1 in a particular preparation.

[0062] Probes are synthesized to specifically hybridize to any of the list of mutations in TABLES 3-7. On each side of the mutation, the probe overlaps at least 3 nucleotides so that the probes specifically hybridize to a DNA with the mutation. Likewise for probes specific to the mutation site with a sequence complementary for the wild type DNA sequence. By using either or both (if the sample is heterozygous) of these probes which differentially hybridize to mutant and wild-type BRCA1 sequences, one can determine the presence or absence of a mutant BRCA1 gene. Probes which hybridize to the complementary strand of the target DNA may also be used in the same manner.

[0063] A pair of isolated allele specific oligonucleotide probes are provided for the mutation 185delAG.

wild-type	5′-AAT CTT AGA GTG TCC CA-3′,	SEQ ID NO:3
mutant	5′-ATC TTA GTG TCC CAC CT-3′,	SEQ ID NO:4

[0064] SEQ ID NO:3 preferentially may be hybridized to a target BRCA1 sequence under conditions where this probe anneals with a wild type BRCA1 gene or gene fragments, whereas SEQ ID NO:4 preferentially may be hybridized to a target BRCA1 sequence under conditions where this probe anneals with BRCA1 gene or gene fragments containing the 185delAG mutation.

[0065] A pair of isolated allele specific oligonucleotide probes are provided for the mutation 1136insA.

wild-type	5′-CAG AAA AAA AGG TAG AT-3′,	SEQ ID NO:5
mutant	5′-CAG AAA AAA AAG GTA GA-3′,	SEQ ID NO:6

[0066] SEQ ID NO:5 preferentially may be hybridized to a target BRCA1 sequence under conditions where this probe anneals with a wild type BRCA1 gene or gene fragments, whereas SEQ ID NO:6 preferentially may be hybridized to a target BRCA1 sequence under conditions where this probe anneals with BRCA1 gene or gene fragments containing the 1136insA mutation.

[0067] A pair of isolated allele specific oligonucleotide probes are provided for the mutation 5382insC.

wild-type	5′-AGA GAA TCC CAG GAC AG-3′,	SEQ ID NO:7
mutant	5′-AGA GAA TCC CCA GGA CA-3′,	SEQ ID NO:8

[0068] SEQ ID NO:9 preferentially may be hybridized to a target BRCA1 sequence under conditions where this probe anneals with a wild type BRCA1 gene or gene fragments, whereas SEQ ID NO:10 preferentially may be hybridized to a target BRCA1 sequence under conditions where this probe anneals with BRCA1 gene or gene fragments containing the 5382insC mutation.

[0069] A pair of isolated allele specific oligonucleotide probes are provided for the mutation C4446T.

wild-type
5′-AGG ACC TGC GAA ATC CA-3′, SEQ ID NO:9
mutant
5′-AGG ACC TGT GAA ATC CA-3′, SEQ ID NO:10

[0070] SEQ ID NO:11 preferentially may be hybridized to a target BRCA1 sequence under conditions where this probe anneals with a wild type BRCA1 gene or gene fragments, whereas SEQ ID NO:12 preferentially may be hybridized to a target BRCA1 sequence under conditions where this probe anneals with BRCA1 gene or gene fragments containing the C4446T mutation. Comparable probes can be prepared for each mutation of the present invention.

[0071] These allele specific oligonucleotides are useful in diagnosis of a subject at risk of having cancer. The allele specific oligonucleotides hybridize with a target polynucleotide sequence containing the mutations listed in TABLES 3-7. The probes having a sequence to naturally occurring (wild-type) BRCA1 hybridize preferentially to the wild type sequence and are useful, for example, as controls. The probes complementary to the sequences containing the mutations listed in TABLES 3-7 are designed to hybridize preferentially to the sequences carrying the specified mutant sequence.

[0072] The primers of the invention embrace oligonucleotides of sufficient length and appropriate sequence so as to provide initiation of polymerization on a significant number of nucleic acids in the mutated locus. Examples of preferred sequences for the primers of the present invention are given in the references cited above.

[0073] Environmental conditions conducive to synthesis of extension products include the presence of nucleoside triphosphates, an agent for polymerization, such as DNA polymerase, and suitable conditions such as temperature, ion composition, ionic strength and pH. The primer is preferably single stranded for maximum efficiency in amplification, but may be double stranded. If double stranded, the primer is preferably first treated to separate its strands before being used to prepare extension products. The primer must be sufficiently long to specifically prime the synthesis of extension products in the presence of the inducing agent for polymerization. The exact length of primer will depend on many factors, including temperature, buffer, and nucleotide composition. The oligonucleotide primer typically contains 13-20 or more nucleotides, although it may contain fewer nucleotides.

[0074] Primers of the invention are designed to be “substantially” complementary to each strand of the genomic locus to be amplified. This means that the primers must be sufficiently complementary to hybridize with their respective strands under conditions which allow the agent for polymerization to perform a polymerase-mediated primer extension reaction. In other words, the primers should have sufficient complementarity with the 5′ and 3′ sequences flanking the mutation to hybridize therewith and permit amplification of the genomic locus. “Substantially” the same as it refers to oligonucleotide sequences which have the functional ability to hybridize or anneal with sufficiently stringent conditions to generate sufficient specificity to distinguish between the presence or absence of the mutation. This is measurable by the temperature of melting being sufficiently different to permit easy identification of whether the oligonucleotide is binding to the normal or mutant BRCA1 gene sequence. Conventional stringency conditions are described, for example, by Sambrook et al, Molecular Cloning, a laboratory Manual, 2nd Edition, Cold Spring Harbor Press, Cold Spring Harbor, N.Y. (1989), more recent editions and Haymes, et al., Nucleic Acid Hybridization, A Practical Approach, IRL Press, Washington, D.C. (1985).

[0075] Oligonucleotide primers of the invention are employed in the amplification process, which is an enzymatic chain reaction that preferably produces exponential quantities of mutated locus relative to the number of reaction steps involved. Typically, one primer is complementary to the negative (−) strand of the mutated locus and the other is complementary to the positive (+) strand. Annealing the primers to denatured nucleic acid is generally followed by extension with an enzyme, such as the large fragment of DNA polymerase I (Klenow) and nucleotides, and results in newly synthesized + and − strands containing the target mutated locus sequence. Because these newly synthesized sequences are also templates, repeated cycles of denaturing, primer annealing, and extension results in exponential production of the region (i.e., the target mutated locus sequence) defined by the primers. The product of the chain reaction is a discreet nucleic acid duplex with termini corresponding to the ends of the specific primers employed.

[0076] The oligonucleotide primers of the invention may be prepared using any suitable method, such as conventional phosphotriester and phosphodiester methods or automated embodiments thereof. In one such automated embodiment, diethylphosphoramidites are used as starting materials and may be synthesized as described by Beaucage, et al., Tetrahedron Letters, 22:1859-1862, (1981). One method for synthesizing oligonucleotides on a modified solid support is described in U.S. Pat. No. 4,458,066.

[0077] Any nucleic acid specimen, in purified or non-purified form, can be utilized as the is starting nucleic acid or acids, providing it contains, or is suspected of containing, the specific nucleic acid sequence containing the mutated locus. Thus, the process may amplify, for example, DNA or RNA, including messenger RNA, wherein DNA or RNA may be single stranded or double stranded. In the event that RNA is to be used as a template, enzymes, and/or conditions optimal for reverse transcribing the template to DNA would preferably be utilized. In addition, a DNA-RNA hybrid which contains one strand of each may be utilized. A mixture of nucleic acids may also be employed, or the nucleic acids produced in a previous amplification reaction herein, using the same or different primers may be so utilized. The specific nucleic acid sequence to be amplified, i.e., the mutated locus, may be a fraction of a larger molecule or can. be present initially as a discrete molecule, so that the specific sequence constitutes the entire nucleic acid. It is not necessary that the sequence to be amplified be present initially in a pure form; it may be a minor fraction of a complex mixture, such as contained in whole human DNA.

[0078] DNA utilized herein may be extracted from a body sample, such as blood, tissue material and the like by a variety of techniques such as that described by Maniatis, et. al. in Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, N.Y., p. 280-281, 1982). If the extracted sample is impure, it may be treated before amplification with an amount of a reagent effective to open the cells, or animal cell membranes of the sample, and to expose and/or separate the strand(s) of the nucleic acid(s). This lysing and nucleic acid denaturing step to expose and separate the strands will allow amplification to occur much more readily.

[0079] The deoxyribonucleotide triphosphates dATP, dCTP, dGTP, and dTTP are added to the synthesis mixture, either separately or together with the primers, in adequate amounts and the resulting solution is heated to about 90′-100° C. from about 1 to 10 minutes, preferably from 1 to 4 minutes. This is sufficient to denature any double strands. After this heating period, the solution is allowed to cool at a rate which is preferable for the primer hybridization. To the cooled mixture is added an appropriate agent for effecting the primer extension reaction (called herein agent for polymerization), and the reaction is allowed to occur under conditions known in the art. The agent for polymerization may also be added together with the other reagents if it is heat stable. This synthesis (or amplification) reaction may occur at room temperature up to a temperature above which the agent for polymerization no longer functions. Thus, for example, if DNA polymerase is used as the agent, the temperature is generally no greater than about 40° C. Thermostable DNA polymerases, such as Taq polymerase, may function at a higher temperature.

[0080] The agent for polymerization may bge any compound or system which will function to accomplish the synthesis of primer extension products, including enzymes. Suitable enzymes for this purpose include, for example, E. coli DNA polymerase I, Klenow fragment of E. coli DNA polymerase, polymerase muteins, reverse transcriptase, other enzymes, including heat-stable enzymes (i e., those enzymes which perform primer extension after being subjected to temperatures sufficiently elevated to cause denaturation), such as Taq polymerase. The suitable enzyme will facilitate combination of the nucleotides in the proper manner to form the primer extension products which are complementary to each nucleic acid strand. Generally, the synthesis will be initiated at the 3′ end of each primer and proceed in the 5′ direction along the template strand, until synthesis terminates, producing molecules of different lengths.

[0081] The newly synthesized strand and its complementary nucleic acid strand will form a double-stranded molecule under hybridizing conditions described above and this hybrid is used in subsequent steps of the process. In the next step, the newly synthesized double-stranded molecule is subjected to denaturing conditions using any of the procedures described above to provide single-stranded molecules.

[0082] The steps of denaturing, annealing, and extension product synthesis can be repeated as often as needed to amplify the target nucleic acid sequence to the extent necessary for detection. The amount of the specific nucleic acid sequence produced will accumulate in an exponential fashion (PCR. A Practical Approach, ILR Press, Eds. M. J. McPherson, P. Quirke, and G. R; Taylor, 1992).

[0083] The amplification products may be detected by Southern blot analysis using non-isotopic detection methods. In such a process, for example, a small sample of DNA containing a very low level of the nucleic acid sequence of the polymorphic locus is amplified, and analyzed via a Southern blotting technique or similarly, using dot blot analysis. The use of non-radioactive probes or labels is facilitated by the high level of the amplified signal. Alternatively, probes used to detect the amplified products can be directly or indirectly detectably labeled, for example, with a radioisotope, a fluorescent compound, a bioluminescent compound, a chemiluminescent compound, a metal chelator or an enzyme. Those of ordinary skill in the art will know of other suitable labels for binding to the probe, or will be able to ascertain such, using routine experimentation. In the preferred embodiment, the amplification products are determinable by separating the mixture on an agarose gel containing ethidium bromide which causes DNA to be fluorescent.

[0084] Sequences amplified by the methods of the invention can be further evaluated, detected, cloned, sequenced, and the like, either in solution or after binding to a solid support, by any method usually applied to the detection of a specific DNA sequence such as PCR, oligomer restriction (Saiki, et.al., Bio/Technology, 3:1008-1012, (1985)), allele-specific oligonucleotide (ASO) probe analysis (Conner, et. al., Proc. Natl. Acad. Sci. U.S.A., 80:278, (1983)), oligonucleotide ligation assays (OLAs) (Landgren, et. al., Science, 241:1007, (1988)), and the like. Molecular techniques for DNA analysis have been reviewed (Landgren, et. al., Science, 242:229-237, (1988)).

[0085] Preferably, the method of amplifying is by PCR, as described herein and as is commonly used by those of ordinary skill in the art. Alternative methods of amplification have been described and can also be employed as long as the BRCA1 locus amplified by PCR using primers of the invention is similarly amplified by the alternative means. Such alternative amplification systems include but are not limited to self-sustained sequence replication, which begins with a short sequence of RNA of interest and a T7 promoter. Reverse transcriptase copies the RNA into cDNA and degrades the RNA, followed by reverse transcriptase polymerizing a second strand of DNA. Another nucleic acid amplification technique is nucleic acid sequence-based amplification (NASBA) which uses reverse transcription and T7 RNA polymerase and incorporates two primers to target its cycling scheme. NASBA can begin with either DNA or RNA and finish with either, and amplifies to 108 copies within 60 to 90 minutes. Alternatively, nucleic acid can be amplified by ligation activated transcription (LAT). LAT works from a single-stranded template with a single primer that is partially single-stranded and partially double-stranded. Amplification is initiated by ligating a cDNA to the promoter oligonucleotide and within a few hours, amplification is 108 to 109 fold. The QB replicase system can be utilized by attaching an RNA sequence called MDV-1 to RNA complementary to a DNA sequence of interest. Upon mixing with a sample, the hybrid RNA finds its complement among the specimen's mRNAs and binds, activating the replicase to copy the tag-along sequence of interest. Another nucleic acid amplification technique, ligase chain reaction (LCR), works by using two differently labeled halves of a sequence of interest which are covalently bonded by ligase in the presence of the contiguous sequence in a sample, forming a new target. The repair chain reaction (RCR) nucleic acid amplification technique uses two complementary and target-specific oligonucleotide probe pairs, thermostable polymerase and ligase, and DNA. nucleotides to geometrically amplify targeted sequences. A 2-base gap separates the oligonucleotide probe pairs, and the RCR fills and joins the gap, mimicking normal DNA repair. Nucleic acid amplification by strand displacement activation (SDA) typically utilizes a short primer containing a recognition site for Hinc II with short overhang on the 5′ end which binds to target DNA. A DNA polymerase fills in the part of the primer opposite the overhang with sulfur-containing adenine analogs. Hinc II is added but only cuts the unmodified DNA strand. A DNA polymerase that lacks 5′ exonuclease activity enters at the cite of the nick and begins to polymerize, displacing the initial primer strand downstream and building a new one which serves as more primer. SDA produces greater than 107-fold amplification in 2 hours at 37° C. Unlike PCR and LCR, SDA does not require instrumented Temperature cycling. Another modification of the PCR is the TAQMAN amplification (PERKIN ELMER) where an oligonucleotide is labeled with a fluorescent and a quencher. This oligonucleotide anneals to the target between the primers so that when one primer is extended, the 5′ nuclease activity of Taq cleaves of the fluorescent label which is then qualitatively detected and quantitatively determined to correspond to the copy number of amplification. Although PCR is the preferred method of amplification in the invention, other methods such as the above can also be used to amplify the BRCA1 locus in accordance with the present invention.

[0086] To sequence the coding region of the BRCA1 gene, each exon is amplified separately using a pair of PCR primers and the resulting PCR products are sequenced in the forward and reverse directions. Any combination of the primers mentioned above which encompass the entire BRCA1 coding region may be used.

[0087] An alternative method for determining whether a truncating mutation is present is the Protein Truncation Assay (PTA). Protein truncation assay enables us to identify three types mutations in a truncated BRCA1 protein: nonsense mutation, frame shift mutation, and splice-site mutations. Nonsense mutations (see TABLE 3) result when a single base change in a codon creates a signal to terminate the production of the protein. These signals or stop codons come in three types: TGA, TAA, TAG. Frame shift mutations (see TABLES 4-7) occur when bases are added or deleted from the normal sequence. Thus, disrupting the reading frame of the protein and causing a stop codon downstream from the alteration. Splice-site mutations occurring at the intron/exon boundaries have the potential of causing the deletion of an entire exon. Examples of protein truncation assays for BRCA1 is mentioned in Furnari et al, Proceedings of the National Academy of Sciences U.S.A., 96: p. 12479-12484 (11-1997) and Tashiro et al, Cancer Research, 57: 3935-3940 (1997).

[0088] Preferably, the Polymerase Chain Reaction (PCR) is performed to amplify the BRCA1 gene copy number. The amplified BRCA1 gene is transcribed and translated in vitro. Detection of truncated proteins is made possible by the use of polyacrylamide gel electrophoresis. The migration of the mutant band on the gel allows for size targeting of the alteration; thus reducing confirmatory sequencing to a minimum.

[0089] In another embodiment of the invention, a method is provided for diagnosing a subject having a predisposition or higher susceptibility to cancer, or other pathology associated with BRCA1 mutations, comprising sequencing a target nucleic acid of a sample from a subject by dideoxy sequencing following amplification of the target nucleic acid. In such an embodiment, one does not even need to use any of the oligonucleotides, either primers or probes as described herein. The BRCA1 gene, or fragments thereof, may be directly cloned and then sequenced. (such as by dideoxy methods) to determine the presence of absence of a mutation. In such a situation, one need only compare the sequence obtained to a naturally occurring (wild type) BRCA1 gene, or a portion thereof.

[0090] In another embodiment of the invention a method is provided for diagnosing a subject having a predisposition or higher susceptibility to cancer comprising contacting a target nucleic acid of a sample from a subject with a reagent that detects the presence of one of the mutations of the present invention and detecting the mutation.

[0091] In yet another embodiment of the invention, a method is provided for determining whether either gene therapy or protein therapy (with normal BRCA1 protein) is appropriate for the prevention or treatment of cancers and other BRCA1 related syndromes. For this method, BRCA1 mutations are assayed for in a biological sample for BRCA1 mutations. When present, the use of gene therapy or protein therapy to prevent cancer in the individual is appropriate. Likewise when BRCA1 mutations are found in tumor cells from a patient, gene therapy or protein therapy is appropriate for that individual.

[0092] In another embodiment of the invention, a method and reagents are provided for repairing the gene mutation in at least some cells by applying an oligomer comprising the sequence of the wild-type probes to repair the individual's genome by triple strand hybridization. See U.S. Pat. Nos. 5,650,316 and 5,624,803 for example. This is a form of gene therapy to correct the defect in either apparently normal tissue or in an active tumor. Gene repair may also be performed on excised tumor cells which may be helpful in determining the preferred therapy to be used, particularly the reagents used for gene therapy. Other forms of gene therapy, such as providing a complete copy of a normal BRCA1 gene may also be used. Some gene therapy techniques specific to BRCA1 are discussed in Furnari et al, Proceedings of the National Academy of Sciences. U.S.A., 96: p. 12479-12484 (11-1997).

[0093] Since the method of the present invention may be applied to detect a mutant BRCA1 gene in a fetus, therapeutic or preventative measures may be possible. Screening of eggs or sperm from heterozygous individuals may permit one to selectively conceive a zygote without the mutant BRCA1 gene since only one half of the sperm or eggs will contain the mutation.

[0094] In another embodiment of the invention a method is provided for characterizing a tumor. Histologic type, morphologic grade, differences between inherited and sporadic cancer appear to be distinguished. One method comprises sequencing the target nucleic acid isolated from the tumor or other biological sample to determine if the mutation is present. Sanger, et al., J. Mol. Biol. 142:1617 (1980).

[0095] Characterizing a tumor as having originated from an inherited gene, a known or suspected cause, or a sporadic cancer gene may be clinically significant as the prevalence of bilateral breast cancer is higher in individuals with a known mutation in a tumor suppressor gene than in sporadic cases. Weber, Scientific American, January-February p. 12-21 (1996). The tumor may be classified based on tissue taken from the tumor itself or from a non-tumor site which contains DNA.

[0096] Yet another embodiment of the present invention is an isolated mutant BRCA1 DNA sequence which may be the entire sequence, an intron, an exon thereof or a fragment or combination thereof. The BRCA1 DNA may be hybridized to an oligonucleotide probe, primer or polynucleotide and still be considered “isolated”. The DNA sequence must contain at least one mutation from the list provided in TABLES 3-7. Preferably, the isolated DNA sequence contains a sequence complementary to at least one of the oligonucleotides complementary to the mutations listed in TABLES 3-7. However, the DNA sequence may contain the DNA sequence of these oligonucleotides. This sequence alone has usefulness or after cloning and expression to determine suitable treatments to prevent formation of a tumor, prevent transmission of the mutant gene to offspring or to decide other prophylactic, diagnostic and treatment protocols. The isolated DNA sequence may also be used for drug design by protein replacement, protein mimetics, screening known and unknown compounds, anti-idiotype antibodies to the BRCA1 active site, for the preparation of an immunogen or vaccine and determining appropriate gene therapy to counter the pathology associated with the mutant BRCA1 gene. For diagnostic purposes, knowing the mutant BRCA1 sequence for comparison purposes is the critical step in diagnosis.

[0097] Another method comprises contacting a target nucleic acid of a sample from a subject with a reagent that detects the presence of the mutation and detecting the mutation. A number of hybridization methods are well known to those skilled in the art. Many of them are useful in carrying out the invention.

[0098] The materials for use in the method of the invention are also ideally suited for the preparation of a diagnostic kit. Such a kit may comprise a carrier means being compartmentalized to receive in close confinement one or more container means such as vials, tubes, and the like, each of the container means comprising one or more of the separate elements to be used in the method. For example, one of the container means may comprise means for amplifying BRCA1 DNA, said means comprising the necessary enzyme(s) and oligonucleotide primers for amplifying said target DNA from the subject. Another container may contain oligonucleotide probes for detecting the presence or absence of a mutation.

[0099] The oligonucleotide primers include primers having a sequences referenced above or primer sequences substantially complementary or substantially homologous thereto. Other primers flanking the BRCA1 locus or a region containing one of the mutation sites may be used. The target flanking 5′ and 3′ polynucleotide sequence include other oligonucleotide primers for amplifying the BRCA1 locus will be known or readily ascertainable to those of skill in the art. See the GENBANK sequences mentioned above where flanking sequences are given.

[0100] Oligonucleotide probes including probes having substantially the sequence complementary to the mutations listed in TABLES 3-7 or complementary sequences are useful. Other oligonucleotide probes which hybridize to one or more of the BRCA1 mutation sites and sequences substantially complementary or homologous thereto may be used. Other oligonucleotide probes for detecting the mutations will be known or readily ascertainable to those of skill in the art.

[0101] The following definitions are provided for the purpose of understanding this invention.

[0102] The term “primer” as used herein refers to a sequence comprising two or more deoxyribonucleotides or ribonucleotides, preferably more than three, and more preferably more than eight and most preferably at least 20 nucleotides of the BRCA1 gene wherein the sequence corresponds to a sequence flanking one of the mutations or wild type sequences of BRCA1 corresponding to the mutation sites. Primers may be used to initiate DNA synthesis via the PCR. Oligonucleotides of the present invention can be used for primer hybridization and others will be known or readily ascertainable to those of skill in the art.

[0103] The term “substantially complementary to” or “substantially the sequence” refers to sequences which hybridize to the sequences provided under stringent conditions and/or sequences having sufficient homology with, such that the allele specific oligonucleotides of the invention hybridize to the sequence.

[0104] “Isolated” as used herein refers to being substantially free of other proteins, lipids, carbohydrates or other materials with which they may be associated. It also refers to being substantially free of polynucleic acids being covalently bound thereto. A DNA may be hybridized to another DNA and still be considered “isolated”, such as being hybridized to a solid phase bound or labeled oligonucleotide probe. Such association is typically either in cellular material or in a synthesis medium.

[0105] “Biological sample” refers to a polynucleotide containing sample originally from a biological source. The sample may be from a living, dead, paraffin-embedded tumor specimen or even archeological source from a variety of tissues and cells. Examples include: body fluid [blood (leukocytes), urine (epithelial cells), saliva, cervical and vaginal secretions, milk . . . ] skin, hair roots/follicle, mucus membrane (e.g. buccal or tongue cell scrapings), cervicovaginal cells (from PAP smear, etc.) internal tissue (normal or tumor), chorionic villus tissue, amniotic cells, placental cells, fetal cells, cord blood, sperm or egg.

[0106] “Coding sequence” or “DNA coding sequence” refers to those portions of a gene which, taken together, code for a peptide (protein), or for which the nucleic acid itself has function. The DNA coding sequence generally encodes the “complete” protein which is one which has the same biological activity as the naturally occurring BRCA1 protein.

[0107] A “target polynucleotide” refers to the nucleotide sequence of interest e.g., the BRCA1 encoding polynucleotide. The nucleotides may be deoxyribonucleotides, ribonucleotides, acyclic derivatives and other functional equivalents such as spacer molecules (inosine, the sugar moiety without a base, etc.) and other molecules which are incorporated by a RNA polymerase, a DNA polymerase or a reverse transcriptase.

[0108] “Consensus” means the most commonly occurring in the population.

[0109] As used herein, a nucleic acid molecule is the “complement” of another nucleic acid molecule if it exhibits complete complementarity. As used herein, molecules are said to exhibit a “complete complementarity” when every nucleotide of one of the molecules is complementary to a nucleotide of the other. Two molecules are said to be “substantially complementary” if they can hybridize to one another with sufficient stability to permit them to remain annealed to one another under at least conventional “high-stringency” conditions. Similarly, the molecules are said to be “partially complementary” if they can hybridize to one another with sufficient stability to permit them to remain annealed to one another under conventional “low-stringency” conditions. Conventional stringency conditions are described, for example, by Sambrook, J., et al., (In: Molecular Cloning, a Laboratory Manual, 2nd Edition, Cold Spring Harbor Press, Cold Spring Harbor, N.Y. (1989)), and by Haymes, B. D., et al. (In: Nucleic Acid Hybridization, A Practical Approach, IRL Press, Washington, D.C. (1985)), both herein incorporated by reference).

[0110] As used herein, an oligonucleotide is said to be capable of “specifically hybridizing” to a complementary target polynucleotide if the two molecules are capable of forming an anti-parallel, double-stranded nucleic acid structure under stringent hybridization conditions, whereas the oligonucleotide is substantially unable to form such a structure when incubated under the same conditions with a target polynucleotide to which the oligonucleotide is not substantially complementary.

[0111] “Sequence variation” as used herein refers to any difference in nucleotide sequence between two different oligonucleotide or polynucleotide sequences.

[0112] “Polymorphism” as used herein refers to a sequence variation in a gene which is not necessarily associated with pathology.

[0113] “Mutation” as used herein refers to an altered genetic sequence which results in the gene coding for a non-functioning protein or a protein with substantially reduced or altered function. Generally, a deleterious mutation is associated with pathology or the potential for pathology. The mutations in the present invention usually involve non-sense and frame shift mutations which cause a truncated (and presumably non-functional) protein to be formed. These truncations are at the terminus of the protein rather than a deletion of one or more amino acids in an internal, non-terminal region of the BRCA1 protein.

[0114] The “mutation site” is the location of the added, deleted or substituted bases in the wild-type or consensus BRCA1 DNA sequence which describes the mutant BRCA1 DNA sequence.

[0115] “Predetermined sequence variation” as used herein refers to a nucleotide sequence that is designed to be different than the corresponding sequence in a reference nucleotide sequence. A predetermined sequence variation can be a known mutation in a BRCA1 gene.

[0116] “BRCA1 gene” refers the published gene sequences, such as those appearing in the GENBANK database under Accession Number, 159546, 2489823, and Y08757. Other different sequences which include polymorphisms and genetic alterations, particularly those which don't cause an amino acid change or which are naturally occurring (wild types), which are not associated with pathology are also considered the BRCA1 gene. The corresponding nucleotides would then be used even if the nucleotide number differs. Generally, the sense strand is referred to. The BRCA1 gene may be in fragments. “Fragments” are segments of the BRCA1 gene, generally about 15 or more nucleotides in length, usually a few hundred or more nucleotides in length and potentially containing the particular mutation site of interest. The complementary strand to the sense strand of the BRCA1 gene (the so-called antisense strand) is also considered the “BRCA1 gene”. While the BRCA1 gene discussed herein is the human BRCA1 gene, the corresponding assays and reagents for the gene in other animals may also be used. The BRCA1 gene includes the coding sequences, non-coding sequences (e.g. introns) and regulatory regions affecting gene expression.

[0117] “Allele specific detection assay” as used herein refers to an assay to detect the presence or absence of a predetermined sequence variation in a test polynucleotide or oligonucleotide by annealing the test polynucleotide or oligonucleotide with a polynucleotide or oligonucleotide of predetermined sequence such that differential DNA sequence based techniques or DNA amplification methods discriminate between normal and mutant. Allele Specific Oligonucleotide hybridization is sometimes referred to ASO or the ASO method.

[0118] “Sequence variation locating assay” as used herein refers to an assay that detects a sequence variation in a test polynucleotide or oligonucleotide and localizes the position of the sequence variation to a sub-region of the test polynucleotide, without necessarily determining the precise base change or position of the sequence variation.

[0119] “Targeted confirmatory sequencing” as used herein refers to sequencing a polynucleotide in the region wherein a sequence variation has been located by a sequence variation locating assay in order to determine the precise base change and/or position of the sequence variation.

[0120] “Probe” includes any oligonucleotide which hybridizes to a BRCA1 or mutant BRCA1 sequence. The probe may be labeled (directly or indirectly) or it may act as a primer such as a PCR primer.

[0121] “Cancer”, “tumor” and “neoplasm” are used interchangeably to refer to certain abnormal cells. The terms are not meant to denote a stage of malignancy.

[0122] The invention in several of its embodiments includes:

[0123] Detection of Predetermined Sequence Variations

[0124] Stage I analysis is used to determine the presence or absence of a predetermined nucleotide sequence variation; preferably a known mutation or set of known mutations in the test gene. In accordance with the invention, such predetermined sequence variations are preferably detected by allele specific hybridization, a sequence-dependent-based technique which permits discrimination between normal and mutant alleles. An allele specific assay is dependent on the differential ability of mismatched nucleotide sequences (e.g., normal:mutant) to hybridize with each other, as compared with matching (e.g., normal:normal or mutant:mutant) sequences.

[0125] Detection of Predetermined Sequence Variations Using Allele Specific Hybridization

[0126] A variety of methods well-known in the art can be used for detection of predetermined sequence variations by allele specific hybridization. Preferably, the test gene is probed with allele specific oligonucleotides (ASOs); and each ASO contains the sequence of a known mutation. ASO analysis detects specific sequence variations in a target polynucleotide fragment by testing the ability of a specific oligonucleotide probe to hybridize to the target polynucleotide fragment. Preferably, the oligonucleotide contains the mutant sequence (or its complement). The presence of a sequence variation in the target sequence is indicated by hybridization between the oligonucleotide probe and the target fragment under conditions in which an oligonucleotide probe containing a normal sequence does not hybridize to the target fragment. A lack of hybridization between the sequence variant (e.g., mutant) oligonucleotide probe and the target polynucleotide fragment indicates the absence of the specific sequence variation (e.g., mutation) in the target fragment. In a preferred embodiment, the test samples are probed in a standard dot blot format. Each region within the test gene that contains the sequence corresponding to the ASO is individually applied to a solid surface, for example, as an individual dot on a membrane. Each individual region can be produced, for example, as a separate PCR amplification product using methods well-known in the art (see, for example, the experimental embodiment set forth in Mullis, U.S. Pat. No. 4,683,202). The use of such a dot blot format is described in detail in the Examples below, detailing the Stage I analysis of the human BRCA1 gene to detect the presence or absence of different known mutations using corresponding ASOs.

[0127] Membrane-based formats that can be used as alternatives to the dot blot format for performing ASO analysis include, but are not limited to, reverse dot blot, MAD (multiplex amplification assay), and multiplex allele-specific diagnostic assay (MASDA).

[0128] In a reverse dot blot format, oligonucleotide or polynucleotide probes having known sequence are immobilized on the solid surface, and are subsequently hybridized with the labeled test polynucleotide sample. In this situation, the primers may be labeled or the NTPs maybe labeled prior to amplification to prepare a labeled test polynucleotide sample. Alternatively, the test polynucleotide sample may be labeled subsequent to isolation and/or synthesis.

[0129] In a multiplex format, individual samples contain multiple target sequences within the test gene, instead of just a single target sequence. For example, multiple PCR products each containing at least one of the ASO target sequences are applied within the same sample dot. Multiple PCR products can be produced simultaneously in a single amplification reaction using the methods of Caskey et al., U.S. Pat. No. 5,582,989. The same blot, therefore, can be probed by each ASO whose corresponding sequence is represented in the sample dots.

[0130] A MASDA format expands the level of complexity of the multiplex format by using multiple ASOs to probe each blot (containing dots with multiple target sequences). This procedure is described in detail in U.S. Pat. No. 5,589,330 by A. P. Shuber, and in Michalowsky et al., American Journal of Human Genetics, 59(4): A272, poster 1573 (October 1996), each of which is incorporated herein by reference in its entirety. First, hybridization between the multiple ASO probe and immobilized sample is detected. This method relies on the prediction that the presence of a mutation among the multiple target sequences in a given dot is sufficiently rare that any positive hybridization signal results from a single ASO within the probe mixture hybridizing with the corresponding mutant target. The hybridizing ASO is then identified by isolating it from the site of hybridization and determining its nucleotide sequence.

[0131] Suitable materials that can be used in the dot blot, reverse dot blot, multiplex, and MASDA formats are well-known in the art and include, but are not limited to nylon and nitrocellulose membranes.

[0132] When the target sequences are produced by PCR amplification, the starting material can be chromosomal DNA in which case the DNA is directly amplified. Alternatively, the starting material can be mRNA, in which case the mRNA is preferably first reversed transcribed into cDNA and then amplified according to the well known technique of RT-PCR (see, for example, to U.S. Pat. No. 5,561,058 by Gelfand et al.).

[0133] The methods described above are suitable for moderate screening of a limited number of sequence variations. However, with the need in molecular diagnosis for rapid, cost effective large scale screening, technologies have developed that integrate the basic concept of ASO, but far exceed the capacity for mutation detection and sample number. These alternative methods to the ones described above include, but are not limited to, large scale chip array sequence-based techniques. The use of large scale arrays allows for the rapid analysis of many sequence variants. A review of the differences in the application and development of chip arrays is covered by Southern, Trends In Genetics, 12: 110-115 (March 1996) and Cheng et al., Molecular Diagnosis, 1:183-200 (Sept. 1996). Several approaches exist involving the manufacture of chip arrays. Differences include, but not restricted to: type of solid support to attach the immobilized oligonucleotides, labeling techniques for identification of variants and changes in the hybridization of the target polynucleotide to the probe.

[0134] A promising methodology for large scale analysis on “DNA chips” is described in detail in Hacia et al., Nature Genetics, 14:441-447 (1996), which is hereby incorporated by reference in its entirety. As described in Hacia et al., high density arrays of over 96,000 oligonucleotides, each 20 nucleotides in length, are immobilized to a single glass or silicon chip using light directed chemical synthesis. Contingent on the number and design of the oligonucleotide probe, potentially every base in a sequence can be interrogated for alterations. Oligonucleotides applied to the chip, therefore, can contain sequence variations that are not yet known to occur in the population, or they can be limited to mutations that are known to occur in the population.

[0135] Prior to hybridization with oligonucleotide probes on the chip, the test sample is preferably isolated, amplified and labeled (e.g. fluorescent markers) by means well known to those skilled in the art. The test polynucleotide sample is then hybridized to the immobilized oligonucleotides. The intensity of hybridization of the target polynucleotide to the immobilized probe is quantitated and compared to a reference sequence. The resulting genetic information can be used in molecular diagnosis.

[0136] A common, but not limiting, utility of the “DNA chip” in molecular diagnosis is screening for known mutations. However, this may impose a limitation to the technique by only looking at mutations that have been described in the field. The present invention allows allele specific hybridization analysis be performed with a far greater number of mutations than 1b previously available. In accordance with the present invention, DNA chips may be constructed with any number of ASO's specific for any number of mutations of the present invention. Such DNA chips may include hundreds, thousands, or more different ASO's, optionally enabling the screening for all possible mutations of the present invention in a single DNA chip. Preferably, DNA chips of the present invention contain about 10 to about 1000, about 100 to about 1,000, about 1,000 to about 10,000, about 10,000 to about 10,0000, or even greater than 100,000 allele specific oligonucleotides specific for the mutations of the present invention. Additionally, such a DNA chip may optionally contain ASO's specific for those missense mutations for which a clinical significance has been established and/or ASO's specific for wild-type BRCA1 DNA sequences. Thus, the efficiency and comprehensiveness of large scale ASO analysis will be broadened, reducing the need for cumbersome end-to-end sequence analysis, not only with known mutations but in a comprehensive manner all mutations which might occur as predicted by the principles accepted, and the cost and time associated with these cumbersome tests will be decreased.

EXAMPLE

[0137] Genomic DNA (at least about 100 ng) is isolated from white blood cells of a subject with a family history of various cancer. Genomic DNA (at least about 100 ng) is also isolated from a wide variety of fresh tumor cells from biopsy, frozen tumor tissue previously surgically removed and tumor cell lines. Dideoxy sequence analysis is performed following polymerase chain reaction amplification of the BRCA1 gene. The primers are the same as used in the references above.

[0138] Each segment of the BRCA1 gene is subjected to direct dideoxy sequence analysis by asymmetric amplification using the polymerase chain reaction (PCR) to generate a single stranded product amplified from this DNA sample. Shuldiner, et al., Handbook of Techniques in Endocrine Research, p. 457-486, DePablo, F., Scanes, C., eds., Academic Press, Inc., 1993. Fluorescent dye is attached for automated sequencing using the TAQ DYE TERMINATOR KIT (PERKIN-ELMER cat# 401628). DNA sequencing is performed in both forward and reverse directions on an APPLIED BIOSYSTEMS, INC. (ABI) automated sequencer (Model 377). The software used for analysis of the resulting data is ASEQUENCE NAVIGATOR@ purchased through ABI.

[0139] The methods of the invention, which can be used to detect sequence variations in any polynucleotide sample, are demonstrated in the Example set forth in this section, for the purpose of illustration, for one gene in particular, namely, the human BRCA1 gene. The BRCA1 coding sequence is approximately 5592 base pairs encoding the 403 amino acids BRCA1 protein.

[0140] Designing an Allele Specific Oligonucleotide (ASO) Probe

[0141] An allele specific oligonucleotide probe is a short, single stranded polynucleotide that is engineered to hybridize exactly to a target sequence under a given set of conditions. Routinely, ASO probes are designed to contain sequences identical to the normal allele and sequence variation respectively. Hybridization of the probe to the target allows for the discrimination of a variant sample. Under stringent conditions, a probe with a variation as simple as a single-base pair will not hybridize to a normal sequence due to a destabilizing effect of the normal-mutant duplex (Ikuta, S. et al, Nucleic Acids Research, 15: 797-811 (1987). For use in this invention, probes are used to discriminate between a wild-type or normal sequence from one that is mutated. Each probe pair contains a polynucleotide sequence that encompassed an area that would identify a selected mutation of the BRCA1 gene.

[0142] The design of an ASO hybridization probe must meet two basic requirements. (Current Protocols in Human Genetics, 9.4, (1995)). First, probes that are used together in the same pool should be around the same length. Although the standard length of a probe is optimally about 17 base pairs, the range can be as short as about 13 or as long as 30 or more. If the mutation contains a long insertion, a longer probe may be desirable. Second, the mismatched region should not be placed at the end of the probe, but approximately in the middle of the sequence. In addition, the placement of a mismatch, in the case of a longer probe, should not be at the end, but at a position that allows strong hybridization and stabilization of the polynucleotide strand. In order to minimize the effects of variations in base composition of the probes, tetramethylammonium chloride may be used as in the ASO hybrid's buffer (Shuber, U.S. Pat. No. 5,633,134). Conventionally, ASO probes are synthesized on a DNA synthesizer. They can be labeled with isotopic or non-isotopic detection agents using means familiar to those of skill in the art. The process outlined in this application for making and using probes can be applicable for other gene sequences.

[0143] Protein Truncation Assay

[0144] PCR Amplification

[0145] BRCA1 is first amplified by PCR from a patient sample using one or more primer sets. A single set of primers may be used to amplify the entire BRCA1 gene or multiple sets of primers may be used. Preferably one need not use a separate set of primers for each exon because the protein expression products are so small that detecting a truncation will be difficult.

[0146] Using the primer sets referenced above in a reaction containing Ex Taq Buffer 10×5.0 mL, dNTP's 2.5 mM 4.0 mL, Forward primer 10 mM 1.0 mL, Reverse primer 10 mM 1.0 mL, TaKaRa Ex Taq (Oncor RR001B) 2.5 U 1.0 mL, Template DNA 100 ng/mL 1.0 mL and OmniPure dH2O to 50 mL final volume. One DNA control (placental DNA), one positive control reaction and three no template control reactions are included for each sample batch.

[0147] PCR for the BRCA1 gene is performed using the following thermocycling conditions (4 linked programs):

	Temperature	Time	#of Cycles
	94° C.	5 min.	1
	94° C. 55° C. 72° C.	30 sec. 1 min. 3 min.	1	35
	72° C.	5 min.	1
	4° C.	hold	1

[0148] 5 m\μL of the PCR product is placed on a 2% agarose gel. On the same gel a DNA 100 BP LADDER (Gibco BRL 15628-019) and a low DNA MASS LADDER (Gibco BRL 10068-013) is placed to verify product size.

[0149] The resulting product is analyzed according to the following rules: l)Each patient sample must show a band of the correct size. If a patient sample demonstrates smearing or multiple bands, the PCR reaction needs to be repeated (no more than three times) until a clean, single band is detected. If no PCR product is visible or if only a weak band is visible, but the placental DNA sample worked well, the sample is reamplified with twice as much template. The volume of the reaction is adjusted appropriately.

[0150] All “No template” (2-3) reactions must not show amplification products of any size. If any one shows any contamination (i.e. specific amplification product), all PCR products should be thrown away and the entire PCR set-up should be repeated after appropriate PCR decontamination procedures have been taken.

[0151] The intensity of the patient sample PCR product is compared with that of the DNA 100 bp ladder. The optimum amount of PCR product on the gel should be 50-100 ng. If less than this is present or if the intensity of the patient sample is less than half the intensity of the placental control sample, repeat the PCR reaction until sufficient quantity is obtained. If no PCR product is visible or if only a weak band is visible, but the placental DNA sample worked well, the patient sample is reamplified with twice as much template DNA.

[0152] The PCR product is precipitated by adding the following to each tube: 3M sodium acetate 30 μL, 20 mg/mL glycogen 2 μL, dH2O 178 μL, and PCR product 90 μL. The reaction is mixed by inverting the tubes 4-6 times. 600 μL of 100% ethanol (200 proof) is added to each tube and the reaction can be placed at −20° C. overnight. The following day, samples are allowed to equilibrate to room temperature before proceeding. The tubes are centrifuged at 13,000 rpm for 15 minutes at room temperature in an IEC microcentrifuge. The supernatant is removed leaving a pellet. 1 mL cold 70% ethanol is added to each tube and centrifuged at 13,000 rpm for 5 minutes at room temperature in the IEC microcentrifuge. The supernatant is again removed leaving the pellet. The tubes are dried by vacuum for 10-15 minutes until no ethanol remains in the tube. Redissolve the pellet in 10 μL of dH2O.

[0153] 1 mL of the PCR product is electrophoresed on a 2% TAE agarose gel, in parallel with a DNA 100 bp ladder and a DNA mass ladder to verify product size and amount of product. The mass of the 1 μL purified PCR product is estimated using the DNA mass ladder as a reference. The band equals one-tenth of the total quantity of purified PCR product. The amount required for the lysate reaction is between 500-750 ng. For example, if the band is the intensity of the 100 ng marker, then the amount needed for the lysate reaction is 5.0-7.5 μL of purified PCR product. If there is less than 500 ng total, the PCR must be repeated.

[0154] In vitro transcription/translation

[0155] This procedure is performed using the TnT Coupled Reticulocyte Lysate system from Promega L4610. Each kit contains the reagents necessary for 80 25 μL translation reactions. This kit allows the synthesis of the specified protein from PCR product.

[0156] The TnT Rabbit Reticulocyte Lysate is thawed in gloved hands and immediately place on ice. The TnT T7 RNA Polymerase and RNase Inhibitor (Boehringer Mannheim 799025) are placed on ice at all times. Solutions are mixed in the following order in a 0.5 mL microcentrifuge tube. Rabbit Reticulocyte Lysate 12.5 μL, TnT Reaction buffer 1.0 μL, TnT T7 RNA Polymerase 0.5 μL, Amino Acid Mixture minus Met (1 mM) 0.5 μL, RNase inhibitor 0.5 μL, per 15.0 ml sample.

[0157] The following are added to a clean labeled 0.5 mL microcentrifuge tube for each reaction: master mix 15.0 μL, 35S methionine (Amersham SJ1015) (1 mCi/100 μL) 2.0 μL, DNA template (500-750 ng) per μL, sdH2O to 25 μL final volume. The reactions are placed in the 30° C. water bath for 1.5 hours.

[0158] Gel Preparation, Run and Handling

[0159] Pre-run the gel (15% Tris-glycine Ready gel (15 well) BioRad 161-0938 or (10 well) BioRad 161-0908) in a Mini Protean II Gel apparatus (BioRad 165-2941) for 15 minutes to equilibrate the gel with the SDS from the running buffer (10×Tris/Glycine/SDS, BioRad 161-0732). Load samples and check the buffer volume during the course of the run to assure that nothing has leaked out. Decreased buffer volume in the middle chamber will interfere with the current. Monitor the progress of the gel running to prevent the lower marker (blue band) from running off the gel. The average running time is 3 hours.

[0160] The gel is rinsed with dH2O after fixing the gel to remove excess salicylic acid and oriented. A mixture of 5.26 mL of beta-mercaptoethanol (BioRad 161-0710) and 94.74 mL of Laemelli sample buffer (BioRad 161-0737) was prepared. The 35S ladder is added as follows in a labeled, 0.5 mL microcentrifuge tube: 7 kD marker in 5 μL, 11 kD marker in 5 μL, 25 kD marker in 2 μL, 74 kD marker in 2 μL. A second labeled tube completes the 35S ladder with: combined lysate 14 μL, sample buffer 32 μL, 100 μM IAA 12 μL, sdH2O 22 mL with a total of 20 μL per gel.

[0161] In a 0.5 μL microcentrifuge tube, the following is added:

[0162] 2 μL lysate reaction, 8 μL sample buffer, 4 μL 100 mM IAA, 6 μL sdH2O, to a total load volume of 20 μL. The samples are heated at 95° C. for 3 minutes and then placed on ice for 2 minutes before loading gel. For every gel run, the following controls are included:

[0163] a. 10 μL of Broad Range Prestained SDS-PAGE standard (BioRad 161-0318) heated to 37° C. to dissolve any precipitated material.

[0164] b. 35S ladder

[0165] c. one negative control per sample

[0166] d. one positive control specific to the fragment under analysis

[0167] During the first 20 minutes, the samples are run at 10 mA (20 mA for two gels). This allows the protein to migrate through the resolving gel at a slower rate. After 20 minutes, the current is increased to 20 mA (40 mA for two gels) for the remainder of the gel run. The buffer volume is checked during the course of the run to assure that nothing has leaked out. Decreased buffer volume in the middle chamber will interfere with the current. The average running time is 3 hours. The lower marker on the polypeptide standard is 7.1 kD (blue band) and should not run off the gel.

[0168] After running the gel, the plastic adhesive is removed from the back of the ready gel and gently peel off the top plate. The gel will remain attached to the back plate. The gel is placed in fixative solution of Isopropanol 250 mL, sdH2O 650 mL and acetic acid 100 mL in a volume sufficient to cover gel for 15 minutes with swirling the gel occasionally by hand or on a rotating plate. The gel is then placed in a fluorogenic agent of salicylic acid 160 g and sdH2O to 1 L final volume, pH adjusted to pH 6.0, in a volume sufficient to cover gel for 15 minutes with swirling the gel occasionally by hand or on a rotating plate. The gel is then rinsed with sdH2O to remove any excess salicylic acid.

[0169] The gel is dried by sandwiching it between two sheets of cellophane and drying it in a gel dryer (BioRad 165-1771) for 1.5-2.5 hours. The dried gel is removed, placed in a film cassette (Fisher IB1502350) taped to a piece of film (Kodak BioMax MR Sigma 870-1302) over the dried gel(s), oriented with Identi-kit tape (Diversified Biotech ID-100) and exposed for 3-5 XD hours at −80° C. The film may be exposed overnight at −20° C. or over the weekend at room temperature if needed.

[0170] Patient samples are compared to the normal genomic DNA fragments. Truncated proteins are possible at any point along the sequence. Therefore, a shift in bands located in any patient sample is an indication of a mutation. There are areas in the sequence where a mutation can occur that are difficult to detect because of the small molecular weight protein formed by the truncation or because a stop signal occurs at the end of the sequence. These mutations are scanned for as follows. The base pairs at the 5′ end of BRCA1 are sequenced. This assures the ability to detect, by means of sequencing, proteins 10 kD or less and the ability to detect, by means of protein truncation, anything greater than 10 kD. A mutation causing a stop codon to occur at the end of the BRCA1 gene is identified by sequencing the final 257 base pairs.

[0171] The truncated BRCA1 protein should be of equal intensity on the gel as the normal BRCA1 protein. When a potential truncated band is identified, the protein must be sized using the 35S radiolabeled marker. For example, if a band appears to be in the area of the 50 kD marker, then the range of inspection for the mutation is between 40-50 kD. The position of the stop signal does not always indicate the position where the mutation occurred. Using the following conversion factor: 270 bp=10 kD, the molecular weight can be converted into base pairs.

[0172] Detailed Method for the Detection of Sequence Variations in Polynucleotides

[0173] Isolation of Genomic DNA

[0174] White blood cells are collected from the patients and genomic DNA is extracted from the white blood cells according to well-known methods (Sambrook, et al., Molecular Cloning, A Laboratory Manual, 2nd Ed., 1989, Cold Spring Harbor Laboratory Press, at 9.16-9.19). Genomic DNA is similarly extracted from a wide variety of fresh tumor cells from biopsy, frozen tumor tissue previously surgically removed and tumor cell lines.

[0175] PCR Amplification for Sequencing

[0176] The genomic DNA is used as a template to amplify a DNA fragment encompassing the site of the mutation to be tested. The 25 μl PCR reaction contains the following components: 1 μl template (100 ng/ ml) DNA, 2.5 μl 10×PCR Buffer (PERKIN-ELMER), 1.5 μl dNTP (2 mM each dATP, dCTP, dGTP, dTTP), 1.5 μl Forward Primer (10 mM), 1.5 μl Reverse Primer (10 mM), 0.5 μl (2.5 U total) AMPLITAQ GOLD™ TAQ DNA POLYMERASE or AMPLITAQ7 TAQ DNA POLYMERASE (PERKIN-ELMER), 1.0 to 5.0 μl (25 mM) MgCl2 (depending on the primer) and distilled water (dH2O) up to 25 μl. All reagents for each exon except the genomic DNA can be combined in a master mix and aliquoted into the reaction tubes as a pooled mixture.

[0177] For each exon analyzed, the following control PCRs are set up:

[0178] (1) “Negative” DNA control (100 ng placental DNA (SIGMA CHEMICAL CO., St. Louis, Mo.)

[0179] (2) Three “no template” controls

[0180] PCR for all exons is performed using the following thermocycling conditions:

	Temperature	Time	Number of Cycles
	90° C.	5 min. (AMPLITAQ)	1
	or	10 min. (GOLD)
	95° C. 55° C. 72° C.	30 sec. 30 sec. 1 min.	2	30 cycles
	72° C.	5 min.		1
	4° C.	hold		1

[0181] Quality Control Agarose Gel of PCR Amplification:

[0182] The quality of the PCR products is examined prior to further analysis by electrophoresing an aliquot of each PCR reaction sample on an agarose gel. 5 μl of each PCR reaction is run on an agarose gel along side a DNA 100 BP DNA LADDER (Gibco BRL cat# 15628-019). The electrophoresed PCR products are analyzed according to the following criteria:

[0183] Each patient sample must show a single band of the size corresponding the number of base pairs expected from the length of the PCR product from the forward primer to the reverse primer. If a patient sample demonstrates smearing or multiple bands, the PCR reaction must be repeated until a clean, single band is detected. If no PCR product is visible or if only a weak band is visible, but the control reactions with placental DNA template produced a robust band, the patient sample should be re-amplified with 2×as much template DNA.

[0184] All three “no template” reactions must show no amplification products. Any PCR product present in these reactions is the result of contamination. If any one of the “no template” reactions shows contamination, all PCR products should be discarded and the entire PCR set of reactions should be repeated after the appropriate PCR decontamination procedures have been taken.

[0185] The optimum amount of PCR product on the gel should be between 50 and 100 ng, which can be determined by comparing the intensity of the patient sample PCR products with that of the DNA ladder. If the patient sample PCR products contain less than 50 to 100 ng, the PCR reaction should be repeated until sufficient quantity is obtained.

[0186] DNA Sequencing

[0187] For DNA sequencing, double stranded PCR products are labeled with four different fluorescent dyes, one specific for each nucleotide, in a cycle sequencing reaction. With Dye Terminator Chemistry, when one of these nucleotides is incorporated into the elongating sequence it causes a termination at that point. Over the course of the cycle sequencing reaction, the dye-labeled nucleotides are incorporated along the length of the PCR product generating many different length fragments.

[0188] The dye-labeled PCR products will separate according to size when electrophoresed through a polyacrylamide gel. At the lower portion of the gel on an ABI automated sequencer, the fragments pass through a region where a laser beam continuously scans across the gel. The laser excites the fluorescent dyes attached to the fragments causing the emission of light at a specific wavelength for each dye. Either a photomultiplier tube (PMT) detects the fluorescent light and converts is into an electrical signal (ABI 373) or the light is collected and separated according to wavelength by a spectrograph onto a cooled, charge coupled device (CCD) camera (ABI 377). In either case the data collection software will collect the signals and store them for subsequent sequence analysis.

[0189] PCR products are first purified for sequencing using a QIAQUICK-SPIN PCR PURIFICATION KIT (QIAGEN #28104). The purified PCR products are labeled by adding primers, fluorescently tagged dNTPs and Taq Polymerase FS in an ABI Prism Dye Terminator Cycle Sequencing Kit (PERKIN ELMER/ABI catalog #02154) in a PERKIN ELMER GENEAMP 9600 thermocycler.

[0190] The amounts of each component are:

	For Samples		For Controls
	Reagent	Volume	Reagent	Volume
	Dye mix	8.0 μL	PGEM	2.0 μL
	Primer (1.6 mM)	2.0 μL	M13	2.0 μL
	PCR product	2.0 μL	Dye mix	8.0 μL
	sdH2O	8.0 μL	sdH2O	8.0 μL

[0191] The thermocycling conditions are:

	Temperature	Time	# of Cycles
	96° C. 50° C. 60° C.	15 sec. 5 sec. 4 min.	3	25
	4° C.	hold		1

[0192] The product is then loaded into a gel and placed into an ABI DNA Sequencer (Models 373A & 377) and run. The sequence obtained is analyzed by comparison to the wild type (reference) sequence using SEQUENCE NAVIGATOR software. When a sequence does not align, it indicates a possible mutation. The DNA sequence is determined in both the forward and reverse directions. All results are provided to a second reader for review.

[0193] Heterozygous/homozygous point mutations and polymorphisms must be seen in both strands. Frame shift mutations will be seen in both strands and must have clear double peaks in frame shift regions to be so identified.

[0194] PCR Amplification for ASO

[0195] The genomic DNA is used as a template to amplify a separate DNA fragment encompassing the site of the mutation to be tested. The 50 ml PCR reaction contains the following components: 1 ml template (100 ng/ ml) DNA, 5.0 ml 10×PCR Buffer (PERKIN-ELMER), 2.5 ml dNTP (2 mM each dATP, dCTP, dGTP, dTTP), 2.5 ml Forward Primer (10 mM), 2.5 ml Reverse Primer (10 mM), 0.5 ml (2.5 U total) AMPLITAQ7 TAQ DNA POLYMERASE or AMPLITAQ GOLD™ DNA POLYMERASE (PERKIN-ELMER), 1.0 to 5.0 ml (25 mM) MgCl2 (depending on the primer) and distilled water (dH2O) up to 50 ml. All reagents for each exon except the genomic DNA can be combined in a master mix and aliquoted into the reaction tubes as a pooled mixture.

[0196] For each exon analyzed, the following control PCRs are set up:

[0197] (1) “Negative” DNA control (100 ng placental DNA (SIGMA CHEMICAL CO., St. Louis, Mo.)

[0198] (2) Three “no template” controls

[0199] PCR for all exons is performed using the following thermocycling conditions:

	Temperature	Time	Number of Cycles
	95° C.	5 min. (AMPLITAQ)	1
		or 10 min. (GOLD)
	95° C. 55° C. 72° C.	30 sec. 30 sec. 1 min.	4	30 cycles
	72° C.	5 min.		1
	4° C.	hold		1

[0200] The quality control agarose gel of PCR amplification is performed as above.

[0201] Binding PCR Products to Nylon Membrane

[0202] The PCR products are denatured no more than 30 minutes prior to binding the PCR products to the nylon membrane. To denature the PCR products, the remaining PCR reaction (45 ml) and the appropriate positive control mutant gene amplification product are diluted to 200 ml final volume with PCR Diluent Solution (500 mM NaOH, 2.0 M NaCl, 25 mM EDTA) and mixed thoroughly. The mixture is heated to 95° C. for 5 minutes, and immediately placed on ice and held on ice until loaded onto dot blotter, as described below.

[0203] The PCR products are bound to 9 cm by 13 cm nylon ZETA PROBE BLOTTING MEMBRANE (BIO-RAD, Hercules, Calif., catalog number 162-0153) using a BIO-RAD dot blotter apparatus. Forceps and gloves are used at all times throughout the ASO analysis to manipulate the membrane, with care taken never to touch the surface of the membrane with bare hands or latex gloves.

[0204] Pieces of 3 MM filter paper [WHATMAN7, Clifton, N.J.] and nylon membrane are pre-wet in 10×SSC prepared fresh from 20×SSC buffer stock. The vacuum apparatus is rinsed thoroughly with dH2O prior to assembly with the membrane. 100 ml of each denatured PCR product is added to the wells of the blotting apparatus. Each row of the blotting apparatus contains a set of reactions for a single exon to be tested, including a placental DNA (negative) control, a synthetic oligonucleotide with the desired mutation or a PCR product from a known mutant sample (positive control), and three no template DNA controls.

[0205] After applying PCR products, the nylon filter is placed DNA side up on a piece of 3 MM filter paper saturated with denaturing solution (1.5 M NaCl, 0.5 M NaOH) for 5 minutes. The membrane is transferred to a piece of 3MM filter paper saturated with neutralizing solution (1 M Tris-HCl, pH 8, 1.5 M NaCl) for 5 minutes. The neutralized membrane is then transferred to a dry 3 MM filter DNA side up, and exposed to ultraviolet light (STRALINKER, STRATAGENE, La Jolla, Calif.) for exactly 45 seconds the fix the DNA to the membrane. This UV crosslinking should be performed within 30 min. of the denaturation/neutralization steps. The nylon membrane is then cut into strips such that each strip contains a single row of blots of one set of reactions for a single exon.

[0206] Hybridizing Labeled Oligonucleotides to the Nylon Membrane

[0207] Prehybridization

[0208] The strip is prehybridized at 52° C. incubation using the HYBAID7 (SAVANT INSTRUMENTS, INC., Holbrook, N.Y.) hybridization oven. 2×SSC (15 to 20 ml) is preheated to 52° C. in a water bath. For each nylon strip, a single piece of nylon mesh cut slightly larger than the nylon membrane strip (approximately 1″×5″) is pre-wet with 2×SSC. Each single nylon membrane is removed from the prehybridization solution and placed on top of the nylon mesh. The membrane/mesh “sandwich” is then transferred onto a piece of Parafilm™. The membrane/mesh sandwich is rolled lengthwise and placed into an appropriate HYBAID7 bottle, such that the rotary action of the HYBAID7 apparatus caused the membrane to unroll. The bottle is capped and gently rolled to cause the membrane/mesh to unroll and to evenly distribute the 2×SSC, making sure that no air bubbles formed between the membrane and mesh or between the mesh and the side of the bottle. The 2×SSC is discarded and replaced with 5 ml TMAC Hybridization Solution, which contains 3 M TMAC (tetramethyl ammoniumchloride—SIGMA T-3411), 100 mM Na3PO4 (pH 6.8), 1 mM EDTA, 5× Denhardt's (1% Ficoll, 1% polyvinylpyrrolidone, 1% BSA (fraction V)), 0.6% SDS, and 100 mg/ml Herring Sperm DNA. The filter strips are prehybridized at 52° C. with medium rotation (approx. 8.5 setting on the HYBAID7 speed control) for at least one hour. Prehybridization can also be performed overnight.

[0209] Labeling Oligonucleotides

[0210] The DNA sequences of the numerous oligonucleotide probes are used to detect the BRCA1 mutation. For each mutation, a mutant and a normal oligonucleotide must be labeled. While only five pairs of oligonucleotide probes are listed below, corresponding oligonucleotides for each mutation may be prepared and used in a similar manner.

mutation 185delAG.
wild-type
5′-AAT CTT AGA GTG TCC CA-3′, SEQ ID NO:3
mutant
5′-ATC TTA GTG TCC CAC CT-3′, SEQ ID NO:4
mutation 1136insA.
wild-type
5′-CAG AAA AAA AGG TAG AT-3′, SEQ ID NO:5
mutant
5′-CAG AAA AAA AAG GTA GA-3′, SEQ ID NO:6
mutation 5383insC.
wild-type
5′-AGA GAA TCC CAG GAC AG-3′, SEQ ID NO:7
mutant
5′-AGA GAA TCC CCA GGA CA-3′, SEQ ID NO:8
mutationC4446T.
wild-type
5′-AGG ACC TGC GAA ATC CA-3′, SEQ ID NO:9
mutant
5′-AGG ACC TGT GAA ATC CA-3′, SEQ ID NO:10

[0211] Each labeling reaction contains 2 ml 5×Kinase buffer (or 1 ml of 10×Kinase buffer), 5 ml gamma-ATP 32P (not more than one week old), 1 μl T4 polynucleotide kinase, 3 μl oligonucleotide (20 mM stock), sterile H2O to 10 μl final volume if necessary. The reactions are incubated at 37° C. for 30 minutes, then at 65° C. for 10 minutes to heat inactivate the kinase. The kinase reaction is diluted with an equal volume (10 μl) of sterile dH2O (distilled water).

[0212] The oligonucleotides are purified on STE MICRO SELECT-D, G-25 spin columns (catalog no. 5303-356769), according to the manufacturer's instructions. The 20 μl synthetic oligonucleotide eluate is diluted with 80 μl dH2O (final volume=100 μl). The amount of radioactivity in the oligonucleotide sample is determined by measuring the radioactive counts per minute (cpm). The total radioactivity must be at least 2 million cpm. For any samples containing less than 2 million cpm total, the labeling reaction is repeated.

[0213] Hybridization with Mutant Oligonucleotides

[0214] Approximately 2-5 million cpm of the labeled mutant oligonucleotide probe is diluted into 5 ml of TMAC hybridization solution, containing 40 μl of 20 mM stock of unlabeled normal oligonucleotide. The probe mix is preheated to 52° C. in the hybridization oven. The pre-hybridization solution is removed from each bottle and replaced with the probe mix. The filter is hybridized for 1 hour at 52° C. with moderate agitation. Following hybridization, the probe mix is decanted into a storage tube and stored at −20° C. The filter is rinsed by adding approximately 20 ml of 2×SSC+0.1% SDS at room temperature and rolling the capped bottle gently for approximately 30 seconds and pouring off the rinse. The filter is then washed with 2×SSC+0. 1% SDS at room temperature for 20 to 30 minutes, with shaking.

[0215] The membrane is removed from the wash and placed on a dry piece of 3MM WHATMAN filter paper then wrapped in one layer of plastic wrap, placed on the autoradiography film, and exposed for about five hours depending upon a survey meter indicating the level of radioactivity. The film is developed in an automatic film processor.

[0216] Control Hybridization with Normal Oligonucleotides

[0217] The purpose of this step is to ensure that the PCR products are transferred efficiently to the nylon membrane.

[0218] Following hybridization with the mutant oligonucleotide, as described in the Examples above, each nylon membrane is washed in 2×SSC, 0.1% SDS for 20 minutes at 65° C. to melt off the mutant oligonucleotide probes. The nylon strips are then prehybridized together in 40 ml of TMAC hybridization solution for at least 1 hour at 52° C. in a shaking water bath. 2-5 million counts of each of the normal labeled oligonucleotide probes plus 40 μl of 20 mM stock of unlabeled normal oligonucleotide are added directly to the container containing the nylon membranes and the prehybridization solution. The filter and probes are hybridized at 52° C. with shaking for at least 1 hour. Hybridization can be performed overnight, if necessary. The hybridization solution is poured off, and the nylon membrane is rinsed in 2×SSC, 0. 1% SDS for 1 minute with gentle swirling by hand. The rinse is poured off and the membrane is washed in 2×SSC, 0.1% SDS at room temperature for 20 minutes with shaking.

[0219] The nylon membrane is removed placed on a dry piece of 3 MM WHATMAN filter paper. The nylon membrane is then wrapped in one layer of plastic wrap and placed on autoradiography film, and exposure is for at least 1 hour.

[0220] For each sample, adequate transfer to the membrane is indicated by a strong autoradiographic hybridization signal. For each sample, an absent or weak signal when hybridized with its normal oligonucleotide, indicates an unsuccessful transfer of PCR product, and it is a false negative. The ASO analysis must be repeated for any sample that did not successfully transfer to the nylon membrane.

[0221] Interpreting Results

[0222] After hybridizing with mutant oligonucleotides, the results for each exon are interpreted as follows:

TABLE 4A
Result	Interpretation	Action
5	6	7		8	All quality controls indicate assay is successful	Record results, dark circles are mutation positive, and all others are negative
(+	(−)	NT	NT	NT	Assay not specific,	Rewash
					mutant oligonucleotide	membrane 30
					hybridizing to normal	minutes longer
					DNA.	at appropriate
(+)	(−)	NT	NT	NT		temperature
					Mutant oligonucleotide	and re-expose.
					probe is either washed	Rehybridize
					off or did not	with remaining
					label well enough,	oligonucleo-
					or PCR product is not	signal, perform
					transferred to	tide labeled
					membrane	If still no
					efficiently.	normal
						oligonucleo-
						tide hyb. as
						per the Exam-
						ples to test
						transfer of PCR
						to membrane.
(+)	(−)	NT	NT	NT
					Positive and negative	Perform
					controls indicate assay	standard clean
					is successful, but PCR	up procedures
(+)	(−)	NT	NT	NT	is contaminated.	for PCR
						contamination.

[0223] After hybridization with normal oligonucleotides, interpret the results as follows:

TABLE 4B
						Results indicate transfer	Record results.
						of PCR products to
						membrane is successful.
(+)	(−)	NT	NT	NT
9		10		11		Results indicate transfer of patient sample #1 is inefficient. May get false negative from this sample	This sample will have to be transferred to another membrane and the assay repeated
(+)	(−)	#1	NT	NT	NT

[0224] The sample #1 should be lighter than the controls. Patient samples containing a mutation are generally heterozygous and will hybridize to both the normal and mutant oligonucleotide probes.

[0225] Data on Specific Mutations

[0226] The following specific mutations are examples of those which are presumed to be clinically significant for typing current cancer cells or a germ line mutation increasing the susceptibility to a tumor. A few of these mutations were also found by others as stated in TABLE 1 above.

TABLE 8
List of Nonsense Mutations
T127A, T127g, G144T, G147T, C153T, C174T, A177T, T184A, T184g, G186T, T191A,
T200A, G204T, T208A, A213T, G216T, A231T, T236A, C251A, A252T, C260A, A267T,
C279T, A282T, A285T, C295A, C295g, C297T, T302A, T307A, T307g, T311A, A312T,
A327T, C339T, G342T, A351T, C360T, G369T, G372T, T379A, A381T, T392A, C399T,
T415A, G417T, T422A, T422G, T434A, T434G, A444T, A447T, G450T, G465T, A474T,
G480T, C495T, C509A, C509G, A510T, A522T, A525T, C534T, G540T, G546T, T559A,
C561T, G564T, C582T, G597T, A606T, A621T, C624T, C633T, C639T, A642T, C656A,
C656G, G660T, T664A, G666T, G681T, A696T, T707A, T707G, C710A, G717T, C723T,
G726T, T730A, T733A, T733g, C735T, C747T, G750T, G762T, T772A, A783T, A786T,
T797A, G798T, G807T, G828T, C837T, T856A, G867T, A870T, G882T, G894T, A897T,
T902A, T902G, C903T, C919A, C919g, T925A, G933T, T941A, C964A, C964g, T967A,
T967g, C969T, G975T, T988A, T988g, T991A, T991g, A999T, A1005T, G1017T, A1020T,
G1026T, T1034A, A1038T, A1044T, C1047T, T1057A, T1057g, C1068T, A1077T, G1081A,
G1082A, G1086T, A1092T, G1095T, T1103A, G1128T, A1131T, A1134T, T1163A, G1164T,
A1167T, A1170T, G1173T, G1177A, G1178A, A1182T, C1185T, A1188T, C1199A, C1201A,
C1201g, G1203T, A1212T, G1221T, G1234A, G1235A, C1257T, A1260T, G1269T, G1273A,
G1274A, A1281T, G1290T, T1297A, T1297g, C1312A, C1312g, G1323T, G1329T, C1333A,
C1333g, A1341T, T1357A, G1371T, G1380T, T1385A, T1385G, C1396A, C1396g, G1398T,
A1401T, T1411A, T1411g, G1431T, T1438A, T1438g, T1445A, A1446T, G1452T, A1455T,
A1467T, C1471A, C1471g, G1476T, G1488T, A1494T, A1506T, T1514A, T1514G, A1518T,
A1521T, T1540A, T1540g, G1554T, G1569T, G1584T, C1590T, C1599T, G1602T, A1620T,
T1624A, T1624g, A1626T, A1632T, A1638T, C1648A, C1648g, G1662T, A1674T, A1677T,
T1687A, C1695T, A1698T, G1707T, C1719T, G1722T, C1731T, G1737T, C1740T, C1749T,
G1779T, A1785T, A1791T, C1806T, G1812T, A1815T, G1833T, C1837A, C1837g, G1842T,
A1845T, G1848T, A1860T, A1866T, G1872T, G1902T, G1908T, T1912A, T1912g, C1927A,
C1927g, A1929T, A1938T, A1941T, A1959T, G1989T, A2004T, T2027A, G2031T, T2035A,
C2037T, T2051A, G2061T, G2064T, A2070T, A2073T, A2076T, A2079T, C2084A, C2084G,
C2088T, A2109T, C2118T, G2127T, A2133T, G2136T, G2148T, A2154T, A2157T, A2166T,
G2175T, C2178T, A2187T, A2190T, G2214T, A2220T, T2224A, T2224g, A2250T, T2255A,
C2257A, C2257g, G2268T, A2274T, G2277T, A2301T, G2304T, G2307T, A2310T, G2313T,
G2316T, A2319T, G2325T, A2334T, G2352T, A2361T, T2374A, T2374g, G2379T, G2382T,
T2392A, C2394T, G2400T, A2403T, G2412T, C2428A, C2428g, T2450A, T2450G, C2457T,
G2460T, C2470A, T2473A, T2473g, G2478T, A2496T, A2502T, G2508T, A2517T, T2522A,
C2529T, T2534A, G2544T, A2553T, G2556T, T2573A, A2577T, A2586T, G2598T, A2607T,
T2612A, T2612G, T2617A, G2619T, G2625T, G2643T, G2655T, G2661T, G2664T, G2670T,
C2682T, T2687A, T2687G, T2689A, C2691T, A2703T, C2710A, C2710g, A2712T, C2718T,
C2722A, C2722g, C2737A, C2737g, G2745T, G2754T, G2757T, G2760T, T2765A, T2794A,
T2794g, A2796T, A2799T, C2802T, A2811T, G2823T, T2828A, G2829T, C2832T, A2835T,
G2838T, G2841T, C2847T, G2850T, A2853T, G2859T, A2871T, C2880T, C2919T, A2922T,
A2928T, A2946T, T2951A, A2958T, G2961T, T2978A, C2983A, C2983g, C2988T, A2994T,
G3003T, G3009T, A3027T, G3033T, T3040A, T3040g, C3042T, T3053A, T3053G, A3078T,
C3082A, C3082g, A3090T, A3096T, T3101A, A3102T, A3105T, G3117T, G3120T, G3129T,
G3132T, C3139A, C3139g, C3145A, C3145g, G3150T, A3153T, G3156T, G3162T, G3168T,
A3213T, G3216T, A3228T, G3231T, C3241A, C3241g, G3255T, G3276T, G3297T, G3315T,
C3324T, G3330T, A3339T, A3345T, A3354T, T3358A, A3372T, T3376A, T3376g, T3385A,
C3387T, G3393T, T3401A, T3401G, A3402T, C3405T, G3417T, T3428A, A3429T, G3438T,
A3444T, A3447T, C3450T, G3453T, T3458A, T3458G, G3459T, G3462T, C3471T, T3500A,
T3500G, C3508A, C3508g, T3517A, T3517g, G3519T, C3522T, G3531T, C3549T, T3557A,
G3561T, T3580A, T3580g, G3591T, A3597T, G3600T, G3618T, A3630T, G3633T, A3654T,
C3663T, A3666T, G3669T, G3672T, T3712A, C3717T, C3725A, C3725G, C3726T, A3729T,
A3738T, A3741T, T3745A, T3745g, G3747T, C3754A, C3754g, G3756T, G3759T, T3766A,
T3766g, G3774T, G3780T, G3783T, C3794A, C3798T, T3805A, T3808A, T3808g, A3816T,
C3837T, G3867T, T3872A, A3879T, G3888T, G3891T, T3898A, T3898g, T3901A, T3901g,
C3904A, C3904g, T3907A, A3909T, T3919A, T3919g, C3929A, C3936T, T3946A, A3951T,
C3960T, G3963T, G3978T, G3981T, A3987T, T3992A, T4003A, C4012A, C4012g, C4014T,
C4019A, G4023T, T4027A, G4029T, T4036A, C4056T, T4069A, A4083T, C4086T, C4098T,
G4104T, C4110T, G4113T, A4131T, G4134T, T4138A, C4144A, C4144g, G4152T, G4155T,
A4158T, G4161T, T4171A, G4173T, G4176T, C4185T, G4188T, G4191T, C4194T, C4207A,
C4207g, T4213A, T4213g, G4218T, T4235A, G4236T, G4242T, G4257T, C4265A, C4267A,
C4267g, C4281T, T4294A, T4294g, C4302T, C4305T, C4320T, A4335T, C4341T, C4344T,
G4347T, G4356T, G4362T, T4372A, T4372g, G4374T, C4377T, C4389T, C4406A, C4406G,
G4437T, C4446T, G4455T, C4458T, C4468A, C4468g, G4470T, A4473T, T4483A, T4483g,
C4489A, C4489g, C4491T, A4494T, G4503T, C4508A, C4508G, C4518T, G4527T, A4545T,
G4551T, A4578T, A4584T, G4587T, G4593T, G4599T, C4606A, C4606g, A4617T, C4622A,
C4627A, C4627g, T4630A, T4630g, G4642A, G4643A, C4646A, C4646G, C4658A, C4671T,
A4677T, C4685A, C4685G, C4692T, G4695T, G4698T, A4707T, G4722T, G4725T, C4728T,
C4731T, G4737T, G4740T, T4759A, G4764T, C4775A, C4775G, T4777A, C4785T, G4794T,
G4797T, C4808A, C4808G, G4812T, G4818T, G4845T, G4860T, A4866T, G4875T, C4879A,
C4879g, C4906A, C4906g, T4918A, A4920T, C4929T, T4933A, A4935T, G4944T, C4953T,
T4994A, T4994G, G5004T, G5007T, G5022T, A5025T, G5031T, T5035A, C5044A, C5044g,
G5049T, A5061T, A5064T, G5097T, G5100T, C5117A, C5117G, A5118T, A5127T, A5130T,
T5146A, T5146g, G5163T, G5166T, A5187T, G5199T, T5210A, G5211T, A5223T, T5228A,
T5228G, G5235T, G5244T, G5247T, A5250T, G5254A, G5255A, T5267A, T5267G, G5272A,
G5273A, C5280T, A5289T, G5292T, A5295T, A5298T, G5310T, G5322T, A5328T, G5331T,
G5346T, A5349T, C5358T, A5367T, C5370T, A5376T, G5379T, C5385T, A5391T, A5394T,
G5412T, T5420A, C5423A, T5426A, T5426G, C5454T, G5460T, G5464A, G5465A, C5472T,
T5480A, A5496T, G5499T, C5506A, C5506g, C5509A, C5509g, C5550T, G5563A, G5564A,
G5568T, C5595T, T5603A, G5604T, C5622T, G5625T, G5629A, G5630A, T5635A, C5654A,
C5654G, C5655T, C5660A, C5661T, G5664T, C5678A, C5678G, C5688T, C5708A, C5708G,
G5710A.

[0227]

TABLE 9
List of One Base deletions
124delA, 125delT, 126delT, 127delT, 128delA, 129delT, 130delC, 131delT, 132delG, 133delC,
134delT, 135delC, 136delT, 137delT, 138delC, 139delG, 140delC, 141delG, 142delT, 143delT,
144delG, 145delA, 146delA, 147delG, 148delA, 149delA, 150delG, 151delT, 152delA, 153delC,
154delA, 155delA, 156delA, 157delA, 158delT, 159delG, 160delT, 161delC, 162delA, 163delT,
164delT, 165delA, 166delA, 167delT, 168delG, 169delC, 170delT, 171delA, 172delT, 173delG,
174delC, 175delA, 176delG, 177delA, 178delA, 179delA, 180delA, 181delT, 182delC, 183delT,
184delT, 185delA, 186delG, 187delA, 188delG, 189delT, 190delG, 191delT, 192delC, 193delC,
194delC, 195delA, 196delT, 197delC, 198delT, 199delG, 200delT, 201delC, 202delT, 203delG,
204delG, 205delA, 206delG, 207delT, 208delT, 209delG, 210delA, 211delT, 212delC, 213delA,
214delA, 215delG, 216delG, 217delA, 218delA, 219delC, 220delC, 221delT, 222delG, 223delT,
224delC, 225delT, 226delC, 227delC, 228delA, 229delC, 230delA, 231delA, 232delA, 233delG,
234delT, 235delG, 236delT, 237delG, 238delA, 239delC, 240delC, 241delA, 242delC, 243delA,
244delT, 245delA, 246delT, 247delT, 248delT, 249delT, 250delG, 251delC, 252delA, 253delA,
254delA, 255delT, 256delT, 257delT, 258delT, 259delG, 260delC, 261delA, 262delT, 263delG,
264delC, 265delT, 266delG, 267delA, 268delA, 269delA, 270delC, 271delT, 272delT, 273delC,
274delT, 275delC, 276delA, 277delA, 278delC, 279delC, 280delA, 281delG, 282delA, 283delA,
284delG, 285delA, 286delA, 287delA, 288delG, 289delG, 290delG, 291delC, 292delC, 293delT,
294delT, 295delC, 296delA, 297delC, 298delA, 299delG, 300delT, 301delG, 302delT, 303delC,
304delC, 305delT, 306delT, 307delT, 308delA, 309delT, 310delG, 311delT, 312delA, 313delA,
314delG, 315delA, 316delA, 317delT, 318delG, 319delA, 320delT, 321delA, 322delT, 323delA,
324delA, 325delC, 326delC, 327delA, 328delA, 329delA, 330delA, 331delG, 332delG,
333delA, 334delG, 335delC, 336delC, 337delT, 338delA, 339delC, 340delA, 341delA, 342delG,
343delA, 344delA, 345delA, 346delG, 347delT, 348delA, 349delC, 350delG, 351delA, 352delG,
353delA, 354delT, 355delT, 356delT, 357delA, 358delG, 359delT, 360delC, 361delA, 362delA,
363delC, 364delT, 365delT, 366delG, 367delT, 368delT, 369delG, 370delA, 371delA, 372delG,
373delA, 374delG, 375delC, 376delT, 377delA, 378delT, 379delT, 380delG, 381delA, 382delA,
383delA, 384delA, 385delT, 386delC, 387delA, 388delT, 389delT, 390delT, 391delG, 392delT,
393delG, 394delC, 395delT, 396delT, 397delT, 398delT, 399delC, 400delA, 401delG, 402delC,
403delT, 404delT, 405delG, 406delA, 407delC, 408delA, 409delC, 410delA, 411delG, 412delG,
413delT, 414delT, 415delT, 416delG, 417delG, 418delA, 419delG, 420delT, 421delA, 422delT,
423delG, 424delC, 425delA, 426delA, 427delA, 428delC, 429delA, 430delG, 431delC, 432delT,
433delA, 434delT, 435delA, 436delA, 437delT, 438delT, 439delT, 440delT, 441delG, 442delC,
443delA, 444delA, 445delA, 446delA, 447delA, 448delA, 449delG, 450delG, 451delA,
452delA, 453delA, 454delA, 455delT, 456delA, 457delA, 458delC, 459delT, 460delC, 461delT,
462delC, 463delC, 464delT, 465delG, 466delA, 467delA, 468delC, 469delA, 470delT, 471delC,
472delT, 473delA, 474delA, 475delA, 476delA, 477delG, 478delA, 479delT, 480delG, 481delA,
482delA, 483delG, 484delT, 485delT, 486delT, 487delC, 488delT, 489delA, 490delT, 491delC,
492delA, 493delT, 494delC, 495delC, 496delA, 497delA, 498delA, 499delG, 500delT, 501delA,
502delT, 503delG, 504delG, 505delG, 506delC, 507delT, 508delA, 509delC, 510delA, 511delG,
512delA, 513delA, 514delA, 515delC, 516delC, 517delG, 518delT, 519delG, 520delC, 521delC,
522delA, 523delA, 524delA, 525delA, 526delG, 527delA, 528delC, 529delT, 530delT, 531delC,
532delT, 533delA, 534delC, 535delA, 536delG, 537delA, 538delG, 539delT, 540delG, 541delA,
542delA, 543delC, 544delC, 545delC, 546delG, 547delA, 548delA, 549delA, 550delA, 551delT,
552delC, 553delC, 554delT, 555delT, 556delC, 557delC, 558delT, 559delT, 560delG, 561delC,
562delA, 563delG, 564delG, 565delA, 566delA, 567delA, 568delC, 569delC, 570delA,
571delG, 572delT, 573delC, 574delT, 575delC, 576delA, 577delG, 578delT, 579delG, 580delT,
581delC, 582delC, 583delA, 584delA, 585delC, 586delT, 587delC, 588delT, 589delC, 590delT,
591delA, 592delA, 593delC, 594delC, 595delT, 596delT, 597delG, 598delG, 599delA, 600delA,
601delC, 602delT, 603delG, 604delT, 605delG, 606delA, 607delG, 608delA, 609delA, 610delC,
611delT, 612delC, 613delT, 614delG, 615delA, 616delG, 617delG, 618delA, 619delC, 620delA,
621delA, 622delA, 623delG, 624delC, 625delA, 626delG, 627delC, 628delG, 629delG,
630delA, 631delT, 632delA, 633delC, 634delA, 635delA, 636delC, 637delC, 638delT, 639delC,
640delA, 641delA, 642delA, 643delA, 644delG, 645delA, 646delC, 647delG, 648delT, 649delC,
650delT, 651delG, 652delT, 653delC, 654delT, 655delA, 656delC, 657delA, 658delT, 659delT,
660delG, 661delA, 662delA, 663delT, 664delT, 665delG, 666delG, 667delG, 668delA, 669delT,
670delC, 671delT, 672delG, 673delA, 674delT, 675delT, 676delC, 677delT, 678delT, 679delC,
680delT, 681delG, 682delA, 683delA, 684delG, 685delA, 686delT, 687delA, 688delC, 689delC,
690delG, 691delT, 692delT, 693delA, 694delA, 695delT, 696delA, 697delA, 698delG, 699delG,
700delC, 701delA, 702delA, 703delC, 704delT, 705delT, 706delA, 707delT, 708delT, 709delG,
710delC, 711delA, 712delG, 713delT, 714delG, 715delT, 716delG, 717delG, 718delG, 719delA,
720delG, 721delA, 722delT, 723delC, 724delA, 725delA, 726delG, 727delA, 728delA, 729delT,
730delT, 731delG, 732delT, 733delT, 734delA, 735delC, 736delA, 737delA, 738delA, 739delT,
740delC, 741delA, 742delC, 743delC, 744delC, 745delC, 746delT, 747delC, 748delA, 749delA,
750delG, 751delG, 752delA, 753delA, 754delC, 755delC, 756delA, 757delG, 758delG,
759delG, 760delA, 761delT, 762delG, 763delA, 764delA, 765delA, 766delT, 767delC, 768delA,
769delG, 770delT, 771delT, 772delT, 773delG, 774delG, 775delA, 776delT, 777delT, 778delC,
779delT, 780delG, 781delC, 782delA, 783delA, 784delA, 785delA, 786delA, 787delA, 788delG,
789delG, 790delC, 791delT, 792delG, 793delC, 794delT, 795delT, 796delG, 797delT, 798delG,
799delA, 800delA, 801delT, 802delT, 803delT, 804delT, 805delC, 806delT, 807delG, 808delA,
809delG, 810delA, 811delC, 812delG, 813delG, 814delA, 815delT, 816delG, 817delT, 818delA,
819delA, 820delC, 821delA, 822delA, 823delA, 824delT, 825delA, 826delC, 827delT, 828delG,
829delA, 830delA, 831delC, 832delA, 833delT, 834delC, 835delA, 836delT, 837delC, 838delA,
839delA, 840delC, 841delC, 842delC, 843delA, 844delG, 845delT, 846delA, 847delA, 848delT,
849delA, 850delA, 851delT, 852delG, 853delA, 854delT, 855delT, 856delT, 857delG, 858delA,
859delA, 860delC, 861delA, 862delC, 863delC, 864delA, 865delC, 866delT, 867delG, 868delA,
869delG, 870delA, 871delA, 872delG, 873delC, 874delG, 875delT, 876delG, 877delC, 878delA,
879delG, 880delC, 881delT, 882delG, 883delA, 884delG, 885delA, 886delG, 887delG, 888delC,
889delA, 890delT, 891delC, 892delC, 893delA, 894delG, 895delA, 896delA, 897delA, 898delA,
899delG, 900delT, 901delA, 902delT, 903delC, 904delA, 905delG, 906delG, 907delG, 908delT,
909delA, 910delG, 911delT, 912delT, 913delC, 914delT, 915delG, 916delT, 917delT, 918delT,
919delC, 920delA, 921delA, 922delA, 923delC, 924delT, 925delT, 926delG, 927delC, 928delA,
929delT, 930delG, 931delT, 932delG, 933delG, 934delA, 935delG, 936delC, 937delC, 938delA,
939delT, 940delG, 941delT, 942delG, 943delG, 944delC, 945delA, 946delC, 947delA, 948delA,
949delA, 950delT, 951delA, 952delC, 953delT, 954delC, 955delA, 956delT, 957delG, 958delC,
959delC, 960delA, 961delG, 962delC, 963delT, 964delC, 965delA, 966delT, 967delT, 968delA,
969delC, 970delA, 971delG, 972delC, 973delA, 974delT, 975delG, 976delA, 977delG, 978delA,
979delA, 980delC, 981delA, 982delG, 983delC, 984delA, 985delG, 986delT, 987delT, 988delT,
989delA, 990delT, 991delT, 992delA, 993delC, 994delT, 995delC, 996delA, 997delC, 998delT,
999delA, 1000delA, 1001delA, 1002delG, 1003delA, 1004delC, 1005delA, 1006delG,
1007delA, 1008delA, 1009delT, 1010delG, 1011delA, 1012delA, 1013delT, 1014delG,
1015delT, 1016delA, 1017delG, 1018delA, 1019delA, 1020delA, 1021delA, 1022delG,
1023delG, 1024delC, 1025delT, 1026delG, 1027delA, 1028delA, 1029delT, 1030delT,
1031delC, 1032delT, 1033delG, 1034delT, 1035delA, 1036delA, 1037delT, 1038delA,
1039delA, 1040delA, 1041delA, 1042delG, 1043delC, 1044delA, 1045delA, 1046delA,
1047delC, 1048delA, 1049delG, 1050delC, 1051delC, 1052delT, 1053delG, 1054delG,
1055delC, 1056delT, 1057delT, 1058delA, 1059delG, 1060delC, 1061delA, 1062delA,
1063delG, 1064delG, 1065delA, 1066delG, 1067delC, 1068delC, 1069delA, 1070delA,
1071delC, 1072delA, 1073delT, 1074delA, 1075delA, 1076delC, 1077delA, 1078delG,
1079delA, 1080delT, 1081delG, 1082delG, 1083delG, 1084delC, 1085delT, 1086delG,
1087delG, 1088delA, 1089delA, 1090delG, 1091delT, 1092delA, 1093delA, 1094delG,
1095delG, 1096delA, 1097delA, 1098delA, 1099delC, 1100delA, 1101delT, 1102delG,
1103delT, 1104delA, 1105delA, 1106delT, 1107delG, 1108delA, 1109delT, 1110delA,
1111delG, 1112delG, 1113delC, 1114delG, 1115delG, 1116delA, 1117delC, 1118delT,
1119delC, 1120delC, 1121delC, 1122delA, 1123delG, 1124delC, 1125delA, 1126delC,
1127delA, 1128delG, 1129delA, 1130delA, 1131delA, 1132delA, 1133delA, 1134delA,
1135delA, 1136delG, 1137delG, 1138delT, 1139delA, 1140delG, 1141delA, 1142delT,
1143delC, 1144delT, 1145delG, 1146delA, 1147delA, 1148delT, 1149delG, 1150delC,
1151delT, 1152delG, 1153delA, 1154delT, 1155delC, 1156delC, 1157delC, 1158delC,
1159delT, 1160delG, 1161delT, 1162delG, 1163delT, 1164delG, 1165delA, 1166delG,
1167delA, 1168delG, 1169delA, 1170delA, 1171delA, 1172delA, 1173delG, 1174delA,
1175delA, 1176delT, 1177delG, 1178delG, 1179delA, 1180delA, 1181delT, 1182delA,
1183delA, 1184delG, 1185delC, 1186delA, 1187delG, 1188delA, 1189delA, 1190delA,
1191delC, 1192delT, 1193delG, 1194delC, 1195delC, 1196delA, 1197delT, 1198delG,
1199delC, 1200delT, 1201delC, 1202delA, 1203delG, 1204delA, 1205delG, 1206delA,
1207delA, 1208delT, 1209delC, 1210delC, 1211delT, 1212delA, 1213delG, 1214delA,
1215delG, 1216delA, 1217delT, 1218delA, 1219delC, 1220delT, 1221delG, 1222delA,
1223delA, 1224delG, 1225delA, 1226delT, 1227delG, 1228delT, 1229delT, 1230delC,
1231delC, 1232delT, 1233delT, 1234delG, 1235delG, 1236delA, 1237delT, 1238delA,
1239delA, 1240delC, 1241delA, 1242delC, 1243delT, 1244delA, 1245delA, 1246delA,
1247delT, 1248delA, 1249delG, 1250delC, 1251delA, 1252delG, 1253delC, 1254delA,
1255delT, 1256delT, 1257delC, 1258delA, 1259delG, 1260delA, 1261delA, 1262delA,
1263delG, 1264delT, 1265delT, 1266delA, 1267delA, 1268delT, 1269delG, 1270delA,
1271delG, 1272delT, 1273delG, 1274delG, 1275delT, 1276delT, 1277delT, 1278delT,
1279delC, 1280delC, 1281delA, 1282delG, 1283delA, 1284delA, 1285delG, 1286delT,
1287delG, 1288delA, 1289delT, 1290delG, 1291delA, 1292delA, 1293delC, 1294delT,
1295delG, 1296delT, 1297delT, 1298delA, 1299delG, 1300delG, 1301delT, 1302delT,
1303delC, 1304delT, 1305delG, 1306delA, 1307delT, 1308delG, 1309delA, 1310delC,
1311delT, 1312delC, 1313delA, 1314delC, 1315delA, 1316delT, 1317delG, 1318delA,
1319delT, 1320delG, 1321delG, 1322delG, 1323delG, 1324delA, 1325delG, 1326delT,
1327delC, 1328delT, 1329delG, 1330delA, 1331delA, 1332delT, 1333delC, 1334delA,
1335delA, 1336delA, 1337delT, 1338delG, 1339delC, 1340delC, 1341delA, 1342delA,
1343delA, 1344delG, 1345delT, 1346delA, 1347delG, 1348delC, 1349delT, 1350delG,
1351delA, 1352delT, 1353delG, 1354delT, 1355delA, 1356delT, 1357delT, 1358delG,
1359delG, 1360delA, 1361delC, 1362delG, 1363delT, 1364delT, 1365delC, 1366delT,
1367delA, 1368delA, 1369delA, 1370delT, 1371delG, 1372delA, 1373delG, 1374delG,
1375delT, 1376delA, 1377delG, 1378delA, 1379delT, 1380delG, 1381delA, 1382delA,
1383delT, 1384delA, 1385delT, 1386delT, 1387delC, 1388delT, 1389delG, 1390delG,
1391delT, 1392delT, 1393delC, 1394delT, 1395delT, 1396delC, 1397delA, 1398delG,
1399delA, 1400delG, 1401delA, 1402delA, 1403delA, 1404delA, 1405delT, 1406delA,
1407delG, 1408delA, 1409delC, 1410delT, 1411delT, 1412delA, 1413delC, 1414delT,
1415delG, 1416delG, 1417delC, 1418delC, 1419delA, 1420delG, 1421delT, 1422delG,
1423delA, 1424delT, 1425delC, 1426delC, 1427delT, 1428delC, 1429delA, 1430delT,
1431delG, 1432delA, 1433delG, 1434delG, 1435delC, 1436delT, 1437delT, 1438delT,
1439delA, 1440delA, 1441delT, 1442delA, 1443delT, 1444delG, 1445delT, 1446delA,
1447delA, 1448delA, 1449delA, 1450delG, 1451delT, 1452delG, 1453delA, 1454delA,
1455delA, 1456delG, 1457delA, 1458delG, 1459delT, 1460delT, 1461delC, 1462delA,
1463delC, 1464delT, 1465delC, 1466delC, 1467delA, 1468delA, 1469delA, 1470delT,
1471delC, 1472delA, 1473delG, 1474delT, 1475delA, 1476delG, 1477delA, 1478delG,
1479delA, 1480delG, 1481delT, 1482delA, 1483delA, 1484delT, 1485delA, 1486delT,
1487delT, 1488delG, 1489delA, 1490delA, 1491delG, 1492delA, 1493delC, 1494delA,
1495delA, 1496delA, 1497delA, 1498delT, 1499delA, 1500delT, 1501delT, 1502delT,
1503delG, 1504delG, 1505delG, 1506delA, 1507delA, 1508delA, 1509delA, 1510delC,
1511delC, 1512delT, 1513delA, 1514delT, 1515delC, 1516delG, 1517delG, 1518delA,
1519delA, 1520delG, 1521delA, 1522delA, 1523delG, 1524delG, 1525delC, 1526delA,
1527delA, 1528delG, 1529delC, 1530delC, 1531delT, 1532delC, 1533delC, 1534delC,
1535delC, 1536delA, 1537delA, 1538delC, 1539delT, 1540delT, 1541delA, 1542delA,
1543delG, 1544delC, 1545delC, 1546delA, 1547delT, 1548delG, 1549delT, 1550delA,
1551delA, 1552delC, 1553delT, 1554delG, 1555delA, 1556delA, 1557delA, 1558delA,
1559delT, 1560delC, 1561delT, 1562delA, 1563delA, 1564delT, 1565delT, 1566delA,
1567delT, 1568delA, 1569delG, 1570delG, 1571delA, 1572delG, 1573delC, 1574delA,
1575delT, 1576delT, 1577delT, 1578delG, 1579delT, 1580delT, 1581delA, 1582delC, 1583delT,
1584delG, 1585delA, 1586delG, 1587delC, 1588delC, 1589delA, 1590delC, 1591delA,
1592delG, 1593delA, 1594delT, 1595delA, 1596delA, 1597delT, 1598delA, 1599delC,
1600delA, 1601delA, 1602delG, 1603delA, 1604delG, 1605delC, 1606delG, 1607delT,
1608delC, 1609delC, 1610delC, 1611delC, 1612delT, 1613delC, 1614delA, 1615delC,
1616delA, 1617delA, 1618delA, 1619delT, 1620delA, 1621delA, 1622delA, 1623delT,
1624delT, 1625delA, 1626delA, 1627delA, 1628delG, 1629delC, 1630delG, 1631delT,
1632delA, 1633delA, 1634delA, 1635delA, 1636delG, 1637delG, 1638delA, 1639delG,
1640delA, 1641delC, 1642delC, 1643delT, 1644delA, 1645delC, 1646delA, 1647delT,
1648delC, 1649delA, 1650delG, 1651delG, 1652delC, 1653delC, 1654delT, 1655delT,
1656delC, 1657delA, 1658delT, 1659delC, 1660delC, 1661delT, 1662delG, 1663delA,
1664delG, 1665delG, 1666delA, 1667delT, 1668delT, 1669delT, 1670delT, 1671delA,
1672delT, 1673delC, 1674delA, 1675delA, 1676delG, 1677delA, 1678delA, 1679delA,
1680delG, 1681delC, 1682delA, 1683delG, 1684delA, 1685delT, 1686delT, 1687delT,
1688delG, 1689delG, 1690delC, 1691delA, 1692delG, 1693delT, 1694delT, 1695delC,
1696delA, 1697delA, 1698delA, 1699delA, 1700delG, 1701delA, 1702delC, 1703delT,
1704delC, 1705delC, 1706delT, 1707delG, 1708delA, 1709delA, 1710delA, 1711delT,
1712delG, 1713delA, 1714delT, 1715delA, 1716delA, 1717delA, 1718delT, 1719delC,
1720delA, 1721delG, 1722delG, 1723delG, 1724delA, 1725delA, 1726delC, 1727delT,
1728delA, 1729delA, 1730delC, 1731delC, 1732delA, 1733delA, 1734delA, 1735delC,
1736delG, 1737delG, 1738delA, 1739delG, 1740delC, 1741delA, 1742delG, 1743delA,
1744delA, 1745delT, 1746delG, 1747delG, 1748delT, 1749delC, 1750delA, 1751delA,
1752delG, 1753delT, 1754delG, 1755delA, 1756delT, 1757delG, 1758delA, 1759delA,
1760delT, 1761delA, 1762delT, 1763delT, 1764delA, 1765delC, 1766delT, 1767delA,
1768delA, 1769delT, 1770delA, 1771delG, 1772delT, 1773delG, 1774delG, 1775delT,
1776delC, 1777delA, 1778delT, 1779delG, 1780delA, 1781delG, 1782delA, 1783delA,
1784delT, 1785delA, 1786delA, 1787delA, 1788delA, 1789delC, 1790delA, 1791delA,
1792delA, 1793delA, 1794delG, 1795delG, 1796delT, 1797delG, 1798delA, 1799delT,
1800delT, 1801delC, 1802delT, 1803delA, 1804delT, 1805delT, 1806delC, 1807delA,
1808delG, 1809delA, 1810delA, 1811delT, 1812delG, 1813delA, 1814delG, 1815delA,
1816delA, 1817delA, 1818delA, 1819delA, 1820delT, 1821delC, 1822delC, 1823delT,
1824delA, 1825delA, 1826delC, 1827delC, 1828delC, 1829delA, 1830delA, 1831delT,
1832delA, 1833delG, 1834delA, 1835delA, 1836delT, 1837delC, 1838delA, 1839delC,
1840delT, 1841delC, 1842delG, 1843delA, 1844delA, 1845delA, 1846delA, 1847delA,
1848delG, 1849delA, 1850delA, 1851delT, 1852delC, 1853delT, 1854delG, 1855delC,
1856delT, 1857delT, 1858delT, 1859delC, 1860delA, 1861delA, 1862delA, 1863delA,
1864delC, 1865delG, 1866delA, 1867delA, 1868delA, 1869delG, 1870delC, 1871delT,
1872delG, 1873delA, 1874delA, 1875delC, 1876delC, 1877delT, 1878delA, 1879delT,
1880delA, 1881delA, 1882delG, 1883delC, 1884delA, 1885delG, 1886delC, 1887delA,
1888delG, 1889delT, 1890delA, 1891delT, 1892delA, 1893delA, 1894delG, 1895delC,
1896delA, 1897delA, 1898delT, 1899delA, 1900delT, 1901delG, 1902delG, 1903delA,
1904delA, 1905delC, 1906delT, 1907delC, 1908delG, 1909delA, 1910delA, 1911delT,
1912delT, 1913delA, 1914delA, 1915delA, 1916delT, 1917delA, 1918delT, 1919delC,
1920delC, 1921delA, 1922delC, 1923delA, 1924delA, 1925delT, 1926delT, 1927delC,
1928delA, 1929delA, 1930delA, 1931delA, 1932delG, 1933delC, 1934delA, 1935delC,
1936delC, 1937delT, 1938delA, 1939delA, 1940delA, 1941delA, 1942delA, 1943delG,
1944delA, 1945delA, 1946delT, 1947delA, 1948delG, 1949delG, 1950delC, 1951delT,
1952delG, 1953delA, 1954delG, 1955delG, 1956delA, 1957delG, 1958delG, 1959delA,
1960delA, 1961delG, 1962delT, 1963delC, 1964delT, 1965delT, 1966delC, 1967delT,
1968delA, 1969delC, 1970delC, 1971delA, 1972delG, 1973delG, 1974delC, 1975delA,
1976delT, 1977delA, 1978delT, 1979delT, 1980delC, 1981delA, 1982delT, 1983delG,
1984delC, 1985delG, 1986delC, 1987delT, 1988delT, 1989delG, 1990delA, 1991delA,
1992delC, 1993delT, 1994delA, 1995delG, 1996delT, 1997delA, 1998delG, 1999delT,
2000delC, 2001delA, 2002delG, 2003delT, 2004delA, 2005delG, 2006delA, 2007delA,
2008delA, 2009delT, 2010delC, 2011delT, 2012delA, 2013delA, 2014delG, 2015delC,
2016delC, 2017delC, 2018delA, 2019delC, 2020delC, 2021delT, 2022delA, 2023delA,
2024delT, 2025delT, 2026delG, 2027delT, 2028delA, 2029delC, 2030delT, 2031delG,
2032delA, 2033delA, 2034delT, 2035delT, 2036delG, 2037delC, 2038delA, 2039delA,
2040delA, 2041delT, 2042delT, 2043delG, 2044delA, 2045delT, 2046delA, 2047delG,
2048delT, 2049delT, 2050delG, 2051delT, 2052delT, 2053delC, 2054delT, 2055delA,
2056delG, 2057delC, 2058delA, 2059delG, 2060delT, 2061delG, 2062delA, 2063delA,
2064delG, 2065delA, 2066delG, 2067delA, 2068delT, 2069delA, 2070delA, 2071delA,
2072delG, 2073delA, 2074delA, 2075delA, 2076delA, 2077delA, 2078delA, 2079delA,
2080delA, 2081delG, 2082delT, 2083delA, 2084delC, 2085delA, 2086delA, 2087delC,
2088delC, 2089delA, 2090delA, 2091delA, 2092delT, 2093delG, 2094delC, 2095delC,
2096delA, 2097delG, 2098delT, 2099delC, 2100delA, 2101delG, 2102delG, 2103delC,
2104delA, 2105delC, 2106delA, 2107delG, 2108delC, 2109delA, 2110delG, 2111delA,
2112delA, 2113delA, 2114delC, 2115delC, 2116delT, 2117delA, 2118delC, 2119delA,
2120delA, 2121delC, 2122delT, 2123delC, 2124delA, 2125delT, 2126delG, 2127delG,
2128delA, 2129delA, 2130delG, 2131delG, 2132delT, 2133delA, 2134delA, 2135delA,
2136delG, 2137delA, 2138delA, 2139delC, 2140delC, 2141delT, 2142delG, 2143delC,
2144delA, 2145delA, 2146delC, 2147delT, 2148delG, 2149delG, 2150delA, 2151delG,
2152delC, 2153delC, 2154delA, 2155delA, 2156delG, 2157delA, 2158delA, 2159delG,
2160delA, 2161delG, 2162delT, 2163delA, 2164delA, 2165delC, 2166delA, 2167delA,
2168delG, 2169delC, 2170delC, 2171delA, 2172delA, 2173delA, 2174delT, 2175delG,
2176delA, 2177delA, 2178delC, 2179delA, 2180delG, 2181delA, 2182delC, 2183delA,
2184delA, 2185delG, 2186delT, 2187delA, 2188delA, 2189delA, 2190delA, 2191delG,
2192delA, 2193delC, 2194delA, 2195delT, 2196delG, 2197delA, 2198delC, 2199delA,
2200delG, 2201delT, 2202delG, 2203delA, 2204delT, 2205delA, 2206delC, 2207delT,
2208delT, 2209delT, 2210delC, 2211delC, 2212delC, 2213delA, 2214delG, 2215delA,
2216delG, 2217delC, 2218delT, 2219delG, 2220delA, 2221delA, 2222delG, 2223delT,
2224delT, 2225delA, 2226delA, 2227delC, 2228delA, 2229delA, 2230delA, 2231delT,
2232delG, 2233delC, 2234delA, 2235delC, 2236delC, 2237delT, 2238delG, 2239delG,
2240delT, 2241delT, 2242delC, 2243delT, 2244delT, 2245delT, 2246delT, 2247delA, 2248delC,
2249delT, 2250delA, 2251delA, 2252delG, 2253delT, 2254delG, 2255delT, 2256delT,
2257delC, 2258delA, 2259delA, 2260delA, 2261delT, 2262delA, 2263delC, 2264delC,
2265delA, 2266delG, 2267delT, 2268delG, 2269delA, 2270delA, 2271delC, 2272delT,
2273delT, 2274delA, 2275delA, 2276delA, 2277delG, 2278delA, 2279delA, 2280delT,
2281delT, 2282delT, 2283delG, 2284delT, 2285delC, 2286delA, 2287delA, 2288delT,
2289delC, 2290delC, 2291delT, 2292delA, 2293delG, 2294delC, 2295delC, 2296delT,
2297delT, 2298delC, 2299delC, 2300delA, 2301delA, 2302delG, 2303delA, 2304delG,
2305delA, 2306delA, 2307delG, 2308delA, 2309delA, 2310delA, 2311delA, 2312delA,
2313delG, 2314delA, 2315delA, 2316delG, 2317delA, 2318delG, 2319delA, 2320delA,
2321delA, 2322delC, 2323delT, 2324delA, 2325delG, 2326delA, 2327delA, 2328delA,
2329delC, 2330delA, 2331delG, 2332delT, 2333delT, 2334delA, 2335delA, 2336delA,
2337delG, 2338delT, 2339delG, 2340delT, 2341delC, 2342delT, 2343delA, 2344delA,
2345delT, 2346delA, 2347delA, 2348delT, 2349delG, 2350delC, 2351delT, 2352delG,
2353delA, 2354delA, 2355delG, 2356delA, 2357delC, 2358delC, 2359delC, 2360delC,
2361delA, 2362delA, 2363delA, 2364delG, 2365delA, 2366delT, 2367delC, 2368delT,
2369delC, 2370delA, 2371delT, 2372delG, 2373delT, 2374delT, 2375delA, 2376delA,
2377delG, 2378delT, 2379delG, 2380delG, 2381delA, 2382delG, 2383delA, 2384delA,
2385delA, 2386delG, 2387delG, 2388delG, 2389delT, 2390delT, 2391delT, 2392delT,
2393delG, 2394delC, 2395delA, 2396delA, 2397delA, 2398delC, 2399delT, 2400delG,
2401delA, 2402delA, 2403delA, 2404delG, 2405delA, 2406delT, 2407delC, 2408delT,
2409delG, 2410delT, 2411delA, 2412delG, 2413delA, 2414delG, 2415delA, 2416delG,
2417delT, 2418delA, 2419delG, 2420delC, 2421delA, 2422delG, 2423delT, 2424delA,
2425delT, 2426delT, 2427delT, 2428delC, 2429delA, 2430delC, 2431delT, 2432delG,
2433delG, 2434delT, 2435delA, 2436delC, 2437delC, 2438delT, 2439delG, 2440delG,
2441delT, 2442delA, 2443delC, 2444delT, 2445delG, 2446delA, 2447delT, 2448delT,
2449delA, 2450delT, 2451delG, 2452delG, 2453delC, 2454delA, 2455delC, 2456delT,
2457delC, 2458delA, 2459delG, 2460delG, 2461delA, 2462delA, 2463delA, 2464delG,
2465delT, 2466delA, 2467delT, 2468delC, 2469delT, 2470delC, 2471delG, 2472delT,
2473delT, 2474delA, 2475delC, 2476delT, 2477delG, 2478delG, 2479delA, 2480delA,
2481delG, 2482delT, 2483delT, 2484delA, 2485delG, 2486delC, 2487delA, 2488delC,
2489delT, 2490delC, 2491delT, 2492delA, 2493delG, 2494delG, 2495delG, 2496delA,
2497delA, 2498delG, 2499delG, 2500delC, 2501delA, 2502delA, 2503delA, 2504delA,
2505delA, 2506delC, 2507delA, 2508delG, 2509delA, 2510delA, 2511delC, 2512delC,
2513delA, 2514delA, 2515delA, 2516delT, 2517delA, 2518delA, 2519delA, 2520delT,
2521delG, 2522delT, 2523delG, 2524delT, 2525delG, 2526delA, 2527delG, 2528delT,
2529delC, 2530delA, 2531delG, 2532delT, 2533delG, 2534delT, 2535delG, 2536delC,
2537delA, 2538delG, 2539delC, 2540delA, 2541delT, 2542delT, 2543delT, 2544delG,
2545delA, 2546delA, 2547delA, 2548delA, 2549delC, 2550delC, 2551delC, 2552delC,
2553delA, 2554delA, 2555delG, 2556delG, 2557delG, 2558delA, 2559delC, 2560delT,
2561delA, 2562delA, 2563delT, 2564delT, 2565delC, 2566delA, 2567delT, 2568delG,
2569delG, 2570delT, 2571delT, 2572delG, 2573delT, 2574delT, 2575delC, 2576delC,
2577delA, 2578delA, 2579delA, 2580delG, 2581delA, 2582delT, 2583delA, 2584delA,
2585delT, 2586delA, 2587delG, 2588delA, 2589delA, 2590delA, 2591delT, 2592delG,
2593delA, 2594delC, 2595delA, 2596delC, 2597delA, 2598delG, 2599delA, 2600delA,
2601delG, 2602delG, 2603delC, 2604delT, 2605delT, 2606delT, 2607delA, 2608delA,
2609delG, 2610delT, 2611delA, 2612delT, 2613delC, 2614delC, 2615delA, 2616delT,
2617delT, 2618delG, 2619delG, 2620delG, 2621delA, 2622delC, 2623delA, 2624delT,
2625delG, 2626delA, 2627delA, 2628delG, 2629delT, 2630delT, 2631delA, 2632delA,
2633delC, 2634delC, 2635delA, 2636delC, 2637delA, 2638delG, 2639delT, 2640delC,
2641delG, 2642delG, 2643delG, 2644delA, 2645delA, 2646delA, 2647delC, 2648delA,
2649delA, 2650delG, 2651delC, 2652delA, 2653delT, 2654delA, 2655delG, 2656delA,
2657delA, 2658delA, 2659delT, 2660delG, 2661delG, 2662delA, 2663delA, 2664delG,
2665delA, 2666delA, 2667delA, 2668delG, 2669delT, 2670delG, 2671delA, 2672delA,
2673delC, 2674delT, 2675delT, 2676delG, 2677delA, 2678delT, 2679delG, 2680delC,
2681delT, 2682delC, 2683delA, 2684delG, 2685delT, 2686delA, 2687delT, 2688delT,
2689delT, 2690delG, 2691delC, 2692delA, 2693delG, 2694delA, 2695delA, 2696delT,
2697delA, 2698delC, 2699delA, 2700delT, 2701delT, 2702delC, 2703delA, 2704delA,
2705delG, 2706delG, 2707delT, 2708delT, 2709delT, 2710delC, 2711delA, 2712delA,
2713delA, 2714delG, 2715delC, 2716delG, 2717delC, 2718delC, 2719delA, 2720delG,
2721delT, 2722delC, 2723delA, 2724delT, 2725delT, 2726delT, 2727delG, 2728delC,
2729delT, 2730delC, 2731delT, 2732delG, 2733delT, 2734delT, 2735delT, 2736delT, 2737delC,
2738delA, 2739delA, 2740delA, 2741delT, 2742delC, 2743delC, 2744delA, 2745delG,
2746delG, 2747delA, 2748delA, 2749delA, 2750delT, 2751delG, 2752delC, 2753delA,
2754delG, 2755delA, 2756delA, 2757delG, 2758delA, 2759delG, 2760delG, 2761delA,
2762delA, 2763delT, 2764delG, 2765delT, 2766delG, 2767delC, 2768delA, 2769delA,
2770delC, 2771delA, 2772delT, 2773delT, 2774delC, 2775delT, 2776delC, 2777delT,
2778delG, 2779delC, 2780delC, 2781delC, 2782delA, 2783delC, 2784delT, 2785delC,
2786delT, 2787delG, 2788delG, 2789delG, 2790delT, 2791delC, 2792delC, 2793delT,
2794delT, 2795delA, 2796delA, 2797delA, 2798delG, 2799delA, 2800delA, 2801delA,
2802delC, 2803delA, 2804delA, 2805delA, 2806delG, 2807delT, 2808delC, 2809delC,
2810delA, 2811delA, 2812delA, 2813delA, 2814delG, 2815delT, 2816delC, 2817delA,
2818delC, 2819delT, 2820delT, 2821delT, 2822delT, 2823delG, 2824delA, 2825delA,
2826delT, 2827delG, 2828delT, 2829delG, 2830delA, 2831delA, 2832delC, 2833delA,
2834delA, 2835delA, 2836delA, 2837delG, 2838delG, 2839delA, 2840delA, 2841delG,
2842delA, 2843delA, 2844delA, 2845delA, 2846delT, 2847delC, 2848delA, 2849delA,
2850delG, 2851delG, 2852delA, 2853delA, 2854delA, 2855delG, 2856delA, 2857delA,
2858delT, 2859delG, 2860delA, 2861delG, 2862delT, 2863delC, 2864delT, 2865delA,
2866delA, 2867delT, 2868delA, 2869delT, 2870delC, 2871delA, 2872delA, 2873delG,
2874delC, 2875delC, 2876delT, 2877delG, 2878delT, 2879delA, 2880delC, 2881delA,
2882delG, 2883delA, 2884delC, 2885delA, 2886delG, 2887delT, 2888delT, 2889delA,
2890delA, 2891delT, 2892delA, 2893delT, 2894delC, 2895delA, 2896delC, 2897delT,
2898delG, 2899delC, 2900delA, 2901delG, 2902delG, 2903delC, 2904delT, 2905delT,
2906delT, 2907delC, 2908delC, 2909delT, 2910delG, 2911delT, 2912delG, 2913delG,
2914delT, 2915delT, 2916delG, 2917delG, 2918delT, 2919delC, 2920delA, 2921delG,
2922delA, 2923delA, 2924delA, 2925delG, 2926delA, 2927delT, 2928delA, 2929delA,
2930delG, 2931delC, 2932delC, 2933delA, 2934delG, 2935delT, 2936delT, 2937delG,
2938delA, 2939delT, 2940delA, 2941delA, 2942delT, 2943delG, 2944delC, 2945delC,
2946delA, 2947delA, 2948delA, 2949delT, 2950delG, 2951delT, 2952delA, 2953delG,
2954delT, 2955delA, 2956delT, 2957delC, 2958delA, 2959delA, 2960delA, 2961delG,
2962delG, 2963delA, 2964delG, 2965delG, 2966delC, 2967delT, 2968delC, 2969delT,
2970delA, 2971delG, 2972delG, 2973delT, 2974delT, 2975delT, 2976delT, 2977delG,
2978delT, 2979delC, 2980delT, 2981delA, 2982delT, 2983delC, 2984delA, 2985delT,
2986delC, 2987delT, 2988delC, 2989delA, 2990delG, 2991delT, 2992delT, 2993delC,
2994delA, 2995delG, 2996delA, 2997delG, 2998delG, 2999delC, 3000delA, 3001delA,
3002delC, 3003delG, 3004delA, 3005delA, 3006delA, 3007delC, 3008delT, 3009delG,
3010delG, 3011delA, 3012delC, 3013delT, 3014delC, 3015delA, 3016delT, 3017delT,
3018delA, 3019delC, 3020delT, 3021delC, 3022delC, 3023delA, 3024delA, 3025delA,
3026delT, 3027delA, 3028delA, 3029delA, 3030delC, 3031delA, 3032delT, 3033delG,
3034delG, 3035delA, 3036delC, 3037delT, 3038delT, 3039delT, 3040delT, 3041delA,
3042delC, 3043delA, 3044delA, 3045delA, 3046delA, 3047delC, 3048delC, 3049delC,
3050delA, 3051delT, 3052delA, 3053delT, 3054delC, 3055delG, 3056delT, 3057delA,
3058delT, 3059delA, 3060delC, 3061delC, 3062delA, 3063delC, 3064delC, 3065delA,
3066delC, 3067delT, 3068delT, 3069delT, 3070delT, 3071delT, 3072delC, 3073delC, 3074delC,
3075delA, 3076delT, 3077delC, 3078delA, 3079delA, 3080delG, 3081delT, 3082delC,
3083delA, 3084delT, 3085delT, 3086delT, 3087delG, 3088delT, 3089delT, 3090delA,
3091delA, 3092delA, 3093delA, 3094delC, 3095delT, 3096delA, 3097delA, 3098delA,
3099delT, 3100delG, 3101delT, 3102delA, 3103delA, 3104delG, 3105delA, 3106delA,
3107delA, 3108delA, 3109delA, 3110delT, 3111delC, 3112delT, 3113delG, 3114delC,
3115delT, 3116delA, 3117delG, 3118delA, 3119delG, 3120delG, 3121delA, 3122delA,
3123delA, 3124delA, 3125delC, 3126delT, 3127delT, 3128delT, 3129delG, 3030delA,
3131delG, 3132delG, 3133delA, 3134delA, 3135delC, 3136delA, 3137delT, 3138delT,
3139delC, 3140delA, 3141delA, 3142delT, 3143delG, 3144delT, 3145delC, 3146delA,
3147delC, 3148delC, 3149delT, 3150delG, 3151delA, 3152delA, 3153delA, 3154delG,
3155delA, 3156delG, 3157delA, 3158delA, 3159delA, 3160delT, 3161delG, 3162delG,
3163delG, 3164delA, 3165delA, 3166delA, 3167delT, 3168delG, 3169delA, 3170delG,
3171delA, 3172delA, 3173delC, 3174delA, 3175delT, 3176delT, 3177delC, 3178delC,
3179delA, 3180delA, 3181delG, 3182delT, 3183delA, 3184delC, 3185delA, 3186delG,
3187delT, 3188delG, 3189delA, 3190delG, 3191delC, 3192delA, 3193delC, 3194delA,
3195delA, 3196delT, 3197delT, 3198delA, 3199delG, 3200delC, 3201delC, 3202delG,
3203delT, 3204delA, 3205delA, 3206delT, 3207delA, 3208delA, 3209delC, 3210delA,
3211delT, 3212delT, 3213delA, 3214delG, 3215delA, 3216delG, 3217delA, 3218delA,
3219delA, 3220delA, 3221delT, 3222delG, 3223delT, 3224delT, 3225delT, 3226delT,
3227delT, 3228delA, 3229delA, 3230delA, 3231delG, 3232delG, 3233delA, 3234delG,
3235delC, 3236delC, 3237delA, 3238delG, 3239delC, 3240delT, 3241delC, 3242delA,
3243delA, 3244delG, 3245delC, 3246delA, 3247delA, 3248delT, 3249delA, 3250delT,
3251delT, 3252delA, 3253delA, 3254delT, 3255delG, 3256delA, 3257delA, 3258delG,
3259delT, 3260delA, 3261delG, 3262delG, 3263delT, 3264delT, 3265delC, 3266delC,
3267delA, 3268delG, 3269delT, 3270delA, 3271delC, 3272delT, 3273delA, 3274delA,
3275delT, 3276delG, 3277delA, 3278delA, 3279delG, 3280delT, 3281delG, 3282delG,
3283delG, 3284delC, 3285delT, 3286delC, 3287delC, 3288delA, 3289delG, 3290delT,
3291delA, 3292delT, 3293delT, 3294delA, 3295delA, 3296delT, 3297delG, 3298delA,
3299delA, 3300delA, 3301delT, 3302delA, 3303delG, 3304delG, 3305delT, 3306delT,
3307delC, 3308delC, 3309delA, 3310delG, 3311delT, 3312delG, 3313delA, 3314delT,
3315delG, 3316delA, 3317delA, 3318delA, 3319delA, 3320delC, 3321delA, 3322delT,
3323delT, 3324delC, 3325delA, 3326delA, 3327delG, 3328delC, 3329delA, 3330delG,
3331delA, 3332delA, 3333delC, 3334delT, 3335delA, 3336delG, 3337delG, 3338delT,
3339delA, 3340delG, 3341delA, 3342delA, 3343delA, 3344delC, 3345delA, 3346delG,
3347delA, 3348delG, 3349delG, 3350delG, 3351delC, 3352delC, 3353delA, 3354delA,
3355delA, 3356delA, 3357delT, 3358delT, 3359delG, 3360delA, 3361delA, 3362delT,
3363delG, 3364delC, 3365delT, 3366delA, 3367delT, 3368delG, 3369delC, 3370delT,
3371delT, 3372delA, 3373delG, 3374delA, 3375delT, 3376delT, 3377delA, 3378delG,
3379delG, 3380delG, 3381delG, 3382delT, 3383delT, 3384delT, 3385delT, 3386delG,
3387delC, 3388delA, 3389delA, 3390delC, 3391delC, 3392delT, 3393delG, 3394delA,
3395delG, 3396delG, 3397delT, 3398delC, 3399delT, 3400delA, 3401delT, 3402delA,
3403delA, 3404delA, 3405delC, 3406delA, 3407delA, 3408delA, 3409delG, 3410delT,
3411delC, 3412delT, 3413delT, 3414delC, 3415delC, 3416delT, 3417delG, 3418delG,
3419delA, 3420delA, 3421delG, 3422delT, 3423delA, 3424delA, 3425delT, 3426delT,
3427delG, 3428delT, 3429delA, 3430delA, 3431delG, 3432delC, 3433delA, 3434delT,
3435delC, 3436delC, 3437delT, 3438delG, 3439delA, 3440delA, 3441delA, 3442delT,
3443delA, 3444delA, 3445delA, 3446delA, 3447delA, 3448delA, 3449delG, 3450delC,
3451delA, 3452delA, 3453delG, 3454delA, 3455delA, 3456delT, 3457delA, 3458delT,
3459delG, 3460delA, 3461delA, 3462delG, 3463delA, 3464delA, 3465delG, 3466delT,
3467delA, 3468delG, 3469delT, 3470delT, 3471delC, 3472delA, 3473delG, 3474delA,
3475delC, 3476delT, 3477delG, 3478delT, 3479delT, 3480delA, 3481delA, 3482delT,
3483delA, 3484delC, 3485delA, 3486delG, 3487delA, 3488delT, 3489delT, 3490delT,
3491delC, 3492delT, 3493delC, 3494delT, 3495delC, 3496delC, 3497delA, 3498delT,
3499delA, 3500delT, 3501delC, 3502delT, 3503delG, 3504delA, 3505delT, 3506delT,
3507delT, 3508delC, 3509delA, 3510delG, 3511delA, 3512delT, 3513delA, 3514delA,
3515delC, 3516delT, 3517delT, 3518delA, 3519delG, 3520delA, 3521delA, 3522delC,
3523delA, 3524delG, 3525delC, 3526delC, 3527delT, 3528delA, 3529delT, 3530delG,
3531delG, 3532delG, 3533delA, 3534delA, 3535delG, 3536delT, 3537delA, 3538delG,
3539delT, 3540delC, 3541delA, 3542delT, 3543delG, 3544delC, 3545delA, 3546delT,
3547delC, 3548delT, 3549delC, 3550delA, 3551delG, 3552delG, 3553delT, 3554delT,
3555delT, 3556delG, 3557delT, 3558delT, 3559delC, 3560delT, 3561delG, 3562delA,
3563delG, 3564delA, 3565delC, 3566delA, 3567delC, 3568delC, 3569delT, 3570delG,
3571delA, 3572delT, 3573delG, 3574delA, 3575delC, 3576delC, 3577delT, 3578delG,
3579delT, 3580delT, 3581delA, 3582delG, 3583delA, 3584delT, 3585delG, 3586delA,
3587delT, 3588delG, 3589delG, 3590delT, 3591delG, 3592delA, 3593delA, 3594delA,
3595delT, 3596delA, 3597delA, 3598delA, 3599delG, 3600delG, 3601delA, 3602delA,
3603delG, 3604delA, 3605delT, 3606delA, 3607delC, 3608delT, 3609delA, 3610delG,
3611delT, 3612delT, 3613delT, 3614delT, 3615delG, 3616delC, 3617delT, 3618delG,
3619delA, 3620delA, 3621delA, 3622delA, 3623delT, 3624delG, 3625delA, 3626delC,
3627delA, 3628delT, 3629delT, 3630delA, 3631delA, 3632delG, 3633delG, 3634delA,
3635delA, 3636delA, 3637delG, 3638delT, 3639delT, 3640delC, 3641delT, 3642delG,
3643delC, 3644delT, 3645delG, 3646delT, 3647delT, 3648delT, 3649delT, 3650delT, 3651delA,
3652delG, 3653delC, 3654delA, 3655delA, 3656delA, 3657delA, 3658delG, 3659delC,
3660delG, 3661delT, 3662delC, 3663delC, 3664delA, 3665delG, 3666delA, 3667delG,
3668delA, 3669delG, 3670delG, 3671delA, 3672delG, 3673delA, 3674delG, 3675delC
3676delT, 3677delT, 3678delA, 3679delG, 3680delC, 3681delA, 3682delG, 3683delG,
3684delA, 3685delG, 3686delT, 3687delC, 3688delC, 3689delT, 3690delA, 3691delG,
3692delC, 3693delC, 3694delC, 3695delT, 3696delT, 3697delT, 3698delC, 3699delA,
3700delC, 3701delC, 3702delC, 3703delA, 3704delT, 3705delA, 3706delC, 3707delA,
3708delC, 3709delA, 3710delT, 3711delT, 3712delT, 3713delG, 3714delG, 3715delC,
3716delT, 3717delC, 3718delA, 3719delG, 3720delG, 3721delG, 3722delT, 3723delT,
3724delA, 3725delC, 3726delC, 3727delG, 3728delA, 3729delA, 3730delG, 3731delA,
3732delG, 3733delG, 3734delG, 3735delG, 3736delC, 3737delC, 3738delA, 3739delA,
3740delG, 3741delA, 3742delA, 3743delA, 3744delT, 3745delT, 3746delA, 3747delG,
3748delA, 3749delG, 3750delT, 3751delC, 3752delC, 3753delT, 3754delC, 3755delA,
3756delG, 3757delA, 3758delA, 3759delG, 3760delA, 3761delG, 3762delA, 3763delA,
3764delC, 3765delT, 3766delT, 3767delA, 3768delT, 3769delC, 3770delT, 3771delA,
3772delG, 3773delT, 3774delG, 3775delA, 3776delG, 3777delG, 3778delA, 3779delT,
3780delG, 3781delA, 3782delA, 3783delG, 3784delA, 3785delG, 3786delC, 3787delT,
3788delT, 3789delC, 3790delC, 3791delC, 3792delT, 3793delG, 3794delC, 3795delT,
3796delT, 3797delC, 3798delC, 3799delA, 3800delA, 3801delC, 3802delA, 3803delC,
3804delT, 3805delT, 3806delG, 3807delT, 3808delT, 3809delA, 3810delT, 3811delT, 3812delT,
3813delG, 3814delG, 3815delT, 3816delA, 3817delA, 3818delA, 3819delG, 3820delT,
3821delA, 3822delA, 3823delA, 3824delC, 3825delA, 3826delA, 3827delT, 3828delA,
3829delT, 3830delA, 3831delC, 3832delC, 3833delT, 3834delT, 3835delC, 3836delT,
3837delC, 3838delA, 3839delG, 3840delT, 3841delC, 3842delT, 3843delA, 3844delC,
3845delT, 3846delA, 3847delG, 3848delG, 3849delC, 3850delA, 3851delT, 3852delA,
3853delG, 3854delC, 3855delA, 3856delC, 3857delC, 3858delG, 3859delT, 3860delT,
3861delG, 3862delC, 3863delT, 3864delA, 3865delC, 3866delC, 3867delG, 3868delA,
3869delG, 3870delT, 3871delG, 3872delT, 3873delC, 3874delT, 3875delG, 3876delT,
3877delC, 3878delT, 3879delA, 3880delA, 3881delG, 3882delA, 3883delA, 3884delC,
3885delA, 3886delC, 3887delA, 3888delG, 3889delA, 3890delG, 3891delG, 3892delA,
3893delG, 3894delA, 3895delA, 3896delT, 3897delT, 3898delT, 3899delA, 3900delT,
3901delT, 3902delA, 3903delT, 3904delC, 3905delA, 3906delT, 3907delT, 3908delG,
3909delA, 3910delA, 3911delG, 3912delA, 3913delA, 3914delT, 3915delA, 3916delG,
3917delC, 3918delT, 3919delT, 3920delA, 3921delA, 3922delA, 3923delT, 3924delG,
3925delA, 3926delC, 3927delT, 3928delG, 3929delC, 3930delA, 3931delG, 3932delT,
3933delA, 3934delA, 3935delC, 3936delC, 3937delA, 3938delG, 3939delG, 3940delT,
3941delA, 3942delA, 3943delT, 3944delA, 3945delT, 3946delT, 3947delG, 3948delG,
3949delC, 3950delA, 3951delA, 3952delA, 3953delG, 3954delG, 3955delC, 3956delA,
3957delT, 3958delC, 3959delT, 3960delC, 3961delA, 3962delG, 3963delG, 3964delA,
3965delA, 3966delC, 3967delA, 3968delT, 3969delC, 3970delA, 3971delC, 3972delC,
3973delT, 3974delT, 3975delA, 3976delG, 3977delT, 3978delG, 3979delA, 3980delG,
3981delG, 3982delA, 3983delA, 3984delA, 3985delC, 3986delA, 3987delA, 3988delA,
3989delA, 3990delT, 3991delG, 3992delT, 3993delT, 3994delC, 3995delT, 3996delG,
3997delC, 3998delT, 3999delA, 4000delG, 4001delC, 4002delT, 4003delT, 4004delG,
4005delT, 4006delT, 4007delT, 4008delT, 4009delC, 4010delT, 4011delT, 4012delC, 4013delA,
4014delC, 4015delA, 4016delG, 4017delT, 4018delG, 4019delC, 4020delA, 4021delG,
4022delT, 4023delG, 4024delA, 4025delA, 4026delT, 4027delT, 4028delG, 4029delG,
4030delA, 4031delA, 4032delG, 4033delA, 4034delC, 4035delT, 4036delT, 4037delG,
4038delA, 4039delC, 4040delT, 4041delG, 4042delC, 4043delA, 4044delA, 4045delA,
4046delT, 4047delA, 4048delC, 4049delA, 4050delA, 4051delA, 4052delC, 4053delA,
4054delC, 4055delC, 4056delC, 4057delA, 4058delG, 4059delG, 4060delA, 4061delT,
4062delC, 4063delC, 4064delT, 4065delT, 4066delT, 4067delC, 4068delT, 4069delT,
4070delG, 4071delA, 4072delT, 4073delT, 4074delG, 4075delG, 4076delT, 4077delT,
4078delC, 4079delT, 4080delT, 4081delC, 4082delC, 4083delA, 4084delA, 4085delA,
4086delC, 4087delA, 4088delA, 4089delA, 4090delT, 4091delG, 4092delA, 4093delG,
4094delG, 4095delC, 4096delA, 4097delT, 4098delC, 4099delA, 4100delG, 4101delT,
4102delC, 4103delT, 4104delG, 4105delA, 4106delA, 4107delA, 4108delG, 4109delC,
4110delC, 4111delA, 4112delG, 4113delG, 4114delG, 4115delA, 4116delG, 4117delT,
4118delT, 4119delG, 4120delG, 4121delT, 4122delC, 4123delT, 4124delG, 4125delA,
4126delG, 4127delT, 4128delG, 4129delA, 4130delC, 4131delA, 4132delA, 4133delG,
4134delG, 4135delA, 4136delA, 4137delT, 4138delT, 4139delG, 4140delG, 4141delT,
4142delT, 4143delT, 4144delC, 4145delA, 4146delG, 4147delA, 4148delT, 4149delG,
4150delA, 4151delT, 4152delG, 4153delA, 4154delA, 4155delG, 4156delA, 4157delA,
4158delA, 4159delG, 4160delA, 4161delG, 4162delG, 4163delA, 4164delA, 4165delC,
4166delG, 4167delG, 4168delG, 4169delC, 4170delT, 4171delT, 4172delG, 4173delG,
4174delA, 4175delA, 4176delG, 4177delA, 4178delA, 4179delA, 4180delA, 4181delT,
4182delA, 4183delA, 4184delT, 4185delC, 4186delA, 4187delA, 4188delG, 4189delA,
4190delA, 4191delG, 4192delA, 4193delG, 4194delC, 4195delA, 4196delA, 4197delA,
4198delG, 4199delC, 4200delA, 4201delT, 4202delG, 4203delG, 4204delA, 4205delT,
4206delT, 4207delC, 4208delA, 4209delA, 4210delA, 4211delC, 4212delT, 4213delT,
4214delA, 4215delG, 4216delG, 4217delT, 4218delG, 4219delA, 4220delA, 4221delG,
4222delC, 4223delA, 4224delG, 4225delC, 4226delA, 4227delT, 4228delC, 4229delT,
4230delG, 4231delG, 4232delG, 4233delT, 4234delG, 4235delT, 4236delG, 4237delA,
4238delG, 4239delA, 4240delG, 4241delT, 4242delG, 4243delA, 4244delA, 4245delA,
4246delC, 4247delA, 4248delA, 4249delG, 4250delC, 4251delG, 4252delT, 4253delC,
4254delT, 4255delC, 4256delT, 4257delG, 4258delA, 4259delA, 4260delG, 4261delA,
4262delC, 4263delT, 4264delG, 4265delC, 4266delT, 4267delC, 4268delA, 4269delG,
4270delG, 4271delG, 4272delC, 4273delT, 4274delA, 4275delT, 4276delC, 4277delC,
4278delT, 4279delC, 4280delT, 4281delC, 4282delA, 4283delG, 4284delA, 4285delG,
4286delT, 4287delG, 4288delA, 4289delC, 4290delA, 4291delT, 4292delT, 4293delT,
4294delT, 4295delA, 4296delA, 4297delC, 4298delC, 4299delA, 4300delC, 4301delT,
4302delC, 4303delA, 4304delG, 4305delC, 4306delA, 4307delG, 4308delA, 4309delG,
4310delG, 4311delG, 4312delA, 4313delT, 4314delA, 4315delC, 4316delC, 4317delA,
4318delT, 4319delG, 4320delC, 4321delA, 4322delA, 4323delC, 4324delA, 4325delT,
4326delA, 4327delA, 4328delC, 4329delC, 4330delT, 4331delG, 4332delA, 4333delT,
4334delA, 4335delA, 4336delA, 4337delG, 4338delC, 4339delT, 4340delC, 4341delC,
4342delA, 4343delG, 4344delC, 4345delA, 4346delG, 4347delG, 4348delA, 4349delA,
4350delA, 4351delT, 4352delG, 4353delG, 4354delC, 4355delT, 4356delG, 4357delA,
4358delA, 4359delC, 4360delT, 4361delA, 4362delG, 4363delA, 4364delA, 4365delG,
4366delC, 4367delT, 4368delG, 4369delT, 4370delG, 4371delT, 4372delT, 4373delA,
4374delG, 4375delA, 4376delA, 4377delC, 4378delA, 4379delG, 4380delC, 4381delA,
4382delT, 4383delG, 4384delG, 4385delG, 4386delA, 4387delG, 4388delC, 4389delC,
4390delA, 4391delG, 4392delC, 4393delC, 4394delT, 4395delT, 4396delC, 4397delT,
4398delA, 4399delA, 4400delC, 4401delA, 4402delG, 4403delC, 4404delT, 4405delA,
4406delC, 4407delC, 4408delC, 4409delT, 4410delT, 4411delC, 4412delC, 4413delA,
4414delT, 4415delC, 4416delA, 4417delT, 4418delA, 4419delA, 4420delG, 4421delT,
4422delG, 4423delA, 4424delC, 4425delT, 4426delC, 4427delC, 4428delT, 4429delC,
4430delT, 4431delG, 4432delC, 4433delC, 4434delC, 4435delT, 4436delT, 4437delG,
4438delA, 4439delG, 4440delG, 4441delA, 4442delC, 4443delC, 4444delT, 4445delG,
4446delC, 4447delG, 4448delA, 4449delA, 4450delA, 4451delT, 4452delC, 4453delC,
4454delA, 4455delG, 4456delA, 4457delA, 4458delC, 4459delA, 4460delA, 4461delA,
4462delG, 4463delC, 4464delA, 4465delC, 4466delA, 4467delT, 4468delC, 4469delA,
4470delG, 4471delA, 4472delA, 4473delA, 4474delA, 4475delA, 4476delG, 4477delC,
4478delA, 4479delG, 4480delT, 4481delA, 4482delT, 4483delT, 4484delA, 4485delA,
4486delC, 4487delT, 4488delT, 4489delC, 4490delA, 4491delC, 4492delA, 4493delG,
4494delA, 4495delA, 4496delA, 4497delA, 4498delG, 4499delT, 4500delA, 4501delG,
4502delT, 4503delG, 4504delA, 4505delA, 4506delT, 4507delA, 4508delC, 4509delC,
4510delC, 4511delT, 4512delA, 4513delT, 4514delA, 4515delA, 4516delG, 4517delC,
4518delC, 4519delA, 4520delG, 4521delA, 4522delA, 4523delT, 4524delC, 4525delC,
4526delA, 4527delG, 4528delA, 4529delA, 4530delG, 4531delG, 4532delC, 4533delC,
4534delT, 4535delT, 4536delT, 4537delC, 4538delT, 4539delG, 4540delC, 4541delT,
4542delG, 4543delA, 4544delC, 4545delA, 4546delA, 4547delG, 4548delT, 4549delT,
4550delT, 4551delG, 4552delA, 4553delG, 4554delG, 4555delT, 4556delG, 4557delT,
4558delC, 4559delT, 4560delG, 4561delC, 4562delA, 4563delG, 4564delA, 4565delT,
4566delA, 4567delG, 4568delT, 4569delT, 4570delC, 4571delT, 4572delA, 4573delC,
4574delC, 4575delA, 4576delG, 4577delT, 4578delA, 4579delA, 4580delA, 4581delA,
4582delA, 4583delT, 4584delA, 4585delA, 4586delA, 4587delG, 4588delA, 4589delA,
4590delC, 4591delC, 4592delA, 4593delG, 4594delG, 4595delA, 4596delG, 4597delT,
4598delG, 4599delG, 4600delA, 4601delA, 4602delA, 4603delG, 4604delG, 4605delT,
4606delC, 4607delA, 4608delT, 4609delC, 4610delC, 4611delC, 4612delC, 4613delT,
4614delT, 4615delC, 4616delT, 4617delA, 4618delA, 4619delA, 4620delT, 4621delG,
4622delC, 4623delC, 4624delC, 4625delA, 4626delT, 4627delC, 4628delA, 4629delT,
4630delT, 4631delA, 4632delG, 4633delA, 4634delT, 4635delG, 4636delA, 4637delT,
4638delA, 4639delG, 4640delG, 4641delT, 4642delG, 4643delG, 4644delT, 4645delA,
4646delC, 4647delA, 4648delT, 4649delG, 4650delC, 4651delA, 4652delC, 4653delA,
4654delG, 4655delT, 4656delT, 4657delG, 4658delC, 4659delT, 4660delC, 4661delT,
4662delG, 4663delG, 4664delG, 4665delA, 4666delG, 4667delT, 4668delC, 4669delT,
4670delT, 4671delC, 4672delA, 4673delG, 4674delA, 4675delA, 4676delT, 4677delA,
4678delG, 4679delA, 4680delA, 4681delA, 4682delC, 4683delT, 4684delA, 4685delC,
4686delC, 4687delC, 4688delA, 4689delT, 4690delC, 4691delT, 4692delC, 4693delA,
4694delA, 4695delG, 4696delA, 4697delG, 4698delG, 4699delA, 4700delG, 4701delC,
4702delT, 4703delC, 4704delA, 4705delT, 4706delT, 4707delA, 4708delA, 4709delG,
4710delG, 4711delT, 4712delT, 4713delG, 4714delT, 4715delT, 4716delG, 4717delA,
4718delT, 4719delG, 4720delT, 4721delG, 4722delG, 4723delA, 4724delG, 4725delG,
4726delA, 4727delG, 4728delC, 4729delA, 4730delA, 4731delC, 4732delA, 4733delG,
4734delC, 4735delT, 4736delG, 4737delG, 4738delA, 4739delA, 4740delG, 4741delA,
4742delG, 4743delT, 4744delC, 4745delT, 4746delG, 4747delG, 4748delG, 4749delC,
4750delC, 4751delA, 4752delC, 4753delA, 4754delC, 4755delG, 4756delA, 4757delT,
4758delT, 4759delT, 4760delG, 4761delA, 4762delC, 4763delG, 4764delG, 4765delA,
4766delA, 4767delA, 4768delC, 4769delA, 4770delT, 4771delC, 4772delT, 4773delT,
4774delA, 4775delC, 4776delT, 4777delT, 4778delG, 4779delC, 4780delC, 4781delA,
4782delA, 4783delG, 4784delG, 4785delC, 4786delA, 4787delA, 4788delG, 4789delA,
4790delT, 4791delC, 4792delT, 4793delA, 4794delG, 4795delA, 4796delG, 4797delG,
4798delG, 4799delA, 4800delA, 4801delC, 4802delC, 4803delC, 4804delC, 4805delT,
4806delT, 4807delA, 4808delC, 4809delC, 4810delT, 4811delG, 4812delG, 4813delA,
4814delA, 4815delT, 4816delC, 4817delT, 4818delG, 4819delG, 4820delA, 4821delA,
4822delT, 4823delC, 4824delA, 4825delG, 4826delC, 4827delC, 4828delT, 4829delC,
4830delT, 4831delT, 4832delC, 4833delT, 4834delC, 4835delT, 4836delG, 4837delA,
4838delT, 4839delG, 4840delA, 4841delC, 4842delC, 4843delC, 4844delT, 4845delG,
4846delA, 4847delA, 4848delT, 4849delC, 4850delT, 4851delG, 4852delA, 4853delT,
4854delC, 4855delC, 4856delT, 4857delT, 4858delC, 4859delT, 4860delG, 4861delA,
4862delA, 4863delG, 4864delA, 4865delC, 4866delA, 4867delG, 4868delA, 4869delG,
4870delC, 4871delC, 4872delC, 4873delC, 4874delA, 4875delG, 4876delA, 4877delG,
4878delT, 4879delC, 4880delA, 4881delG, 4882delC, 4883delT, 4884delC, 4885delG,
4886delT, 4887delG, 4888delT, 4889delT, 4890delG, 4891delG, 4892delC, 4893delA,
4894delA, 4895delC, 4896delA, 4897delT, 4898delA, 4899delC, 4900delC, 4901delA,
4902delT, 4903delC, 4904delT, 4905delT, 4906delC, 4907delA, 4908delA, 4909delC,
4910delC, 4911delT, 4912delC, 4913delT, 4914delG, 4915delC, 4916delA, 4917delT,
4918delT, 4919delG, 4920delA, 4921delA, 4922delA, 4923delG, 4924delT, 4925delT,
4926delC, 4927delC, 4928delC, 4929delC, 4930delA, 4931delA, 4932delT, 4933delT,
4934delG, 4935delA, 4936delA, 4937delA, 4938delG, 4939delT, 4940delT, 4941delG,
4942delC, 4943delA, 4944delG, 4945delA, 4946delA, 4947delT, 4948delC, 4949delT,
4950delG, 4951delC, 4952delC, 4953delC, 4954delA, 4955delG, 4956delG, 4957delG,
4958delT, 4959delC, 4960delC, 4961delA, 4962delG, 4963delC, 4964delT, 4965delG,
4966delC, 4967delT, 4968delG, 4969delC, 4970delT, 4971delC, 4972delA, 4973delT,
4974delA, 4975delC, 4976delT, 4977delA, 4978delC, 4979delT, 4980delG, 4981delA,
4982delT, 4983delA, 4984delC, 4985delT, 4986delG, 4987delC, 4988delT, 4989delG,
4990delG, 4991delG, 4992delT, 4993delA, 4994delT, 4995delA, 4996delA, 4997delT,
4998delG, 4999delC, 5000delA, 5001delA, 5002delT, 5003delG, 5004delG, 5005delA,
5006delA, 5007delG, 5008delA, 5009delA, 5010delA, 5011delG, 5012delT, 5013delG,
5014delT, 5015delG, 5016delA, 5017delG, 5018delC, 5019delA, 5020delG, 5021delG,
5022delG, 5023delA, 5024delG, 5025delA, 5026delA, 5027delG, 5028delC, 5029delC,
5030delA, 5031delG, 5032delA, 5033delA, 5034delT, 5035delT, 5036delG, 5037delA,
5038delC, 5039delA, 5040delG, 5041delC, 5042delT, 5043delT, 5044delC, 5045delA,
5046delA, 5047delC, 5048delA, 5049delG, 5050delA, 5051delA, 5052delA, 5053delG,
5054delG, 5055delG, 5056delT, 5057delC, 5058delA, 5059delA, 5060delC, 5061delA,
5062delA, 5063delA, 5064delA, 5065delG, 5066delA, 5067delA, 5068delT, 5069delG,
5070delT, 5071delC, 5072delC, 5073delA, 5074delT, 5075delG, 5076delG, 5077delT,
5078delG, 5079delG, 5080delT, 5081delG, 5082delT, 5083delC, 5084delT, 5085delG,
5086delG, 5087delC, 5088delC, 5089delT, 5090delG, 5091delA, 5092delC, 5093delC,
5094delC, 5095delC, 5096delA, 5097delG, 5098delA, 5099delA, 5100delG, 5101delA,
5102delA, 5103delT, 5104delT, 5105delT, 5106delA, 5107delT, 5108delG, 5109delC,
5110delT, 5111delC, 5112delG, 5113delT, 5114delG, 5115delT, 5116delA, 5117delC,
5118delA, 5119delA, 5120delG, 5121delT, 5122delT, 5123delT, 5124delG, 5125delC,
5126delC, 5127delA, 5128delG, 5129delA, 5130delA, 5131delA, 5132delA, 5133delC,
5134delA, 5135delC, 5136delC, 5137delA, 5138delC, 5139delA, 5140delT, 5141delC,
5142delA, 5143delC, 5144delT, 5145delT, 5146delT, 5147delA, 5148delA, 5149delC,
5150delT, 5151delA, 5152delA, 5153delT, 5154delC, 5155delT, 5156delA, 5157delA,
5158delT, 5159delT, 5160delA, 5161delC, 5162delT, 5163delG, 5164delA, 5165delA,
5166delG, 5167delA, 5168delG, 5169delA, 5170delC, 5171delT, 5172delA, 5173delC,
5174delT, 5175delC, 5176delA, 5177delT, 5178delG, 5179delT, 5180delT, 5181delG,
5182delT, 5183delT, 5184delA, 5185delT, 5186delG, 5187delA, 5188delA, 5189delA,
5190delA, 5191delC, 5192delA, 5193delG, 5194delA, 5195delT, 5196delG, 5197delC,
5198delT, 5199delG, 5200delA, 5201delG, 5202delT, 5203delT, 5204delT, 5205delG,
5206delT, 5207delG, 5208delT, 5209delG, 5210delT, 5211delG, 5212delA, 5213delA,
5214delC, 5215delG, 5216delG, 5217delA, 5218delC, 5219delA, 5220delC, 5221delT,
5222delG, 5223delA, 5224delA, 5225delA, 5226delT, 5227delA, 5228delT, 5229delT,
5230delT, 5231delT, 5232delC, 5233delT, 5234delA, 5235delG, 5236delG, 5237delA,
5238delA, 5239delT, 5240delT, 5241delG, 5242delC, 5243delG, 5244delG, 5245delG,
5246delA, 5247delG, 5248delG, 5249delA, 5250delA, 5251delA, 5252delA, 5253delT,
5254delG, 5255delG, 5256delG, 5257delT, 5258delA, 5259delG, 5260delT, 5261delT,
5262delA, 5263delG, 5264delC, 5265delT, 5266delA, 5267delT, 5268delT, 5269delT,
5270delC, 5271delT, 5272delG, 5273delG, 5274delG, 5275delT, 5276delG, 5277delA,
5278delC, 5279delC, 5280delC, 5281delA, 5282delG, 5283delT, 5284delC, 5285delT,
5286delA, 5287delT, 5288delT, 5289delA, 5290delA, 5291delA, 5292delG, 5293delA,
5294delA, 5295delA, 5296delG, 5297delA, 5298delA, 5299delA, 5300delA, 5301delA,
5302delT, 5303delG, 5304delC, 5305delT, 5306delG, 5307delA, 5308delA, 5309delT,
5310delG, 5311delA, 5312delG, 5313delC, 5314delA, 5315delT, 5316delG, 5317delA,
5318delT, 5319delT, 5320delT, 5321delT, 5322delG, 5323delA, 5324delA, 5325delG,
5326delT, 5327delC, 5328delA, 5329delG, 5330delA, 5331delG, 5332delG, 5333delA,
5334delG, 5335delA, 5336delT, 5337delG, 5338delT, 5339delG, 5340delG, 5341delT,
5342delC, 5343delA, 5344delA, 5345delT, 5346delG, 5347delG, 5348delA, 5349delA,
5350delG, 5351delA, 5352delA, 5353delA, 5354delC, 5355delC, 5356delA, 5357delC,
5358delC, 5359delA, 5360delA, 5361delG, 5362delG, 5363delT, 5364delC, 5365delC,
5366delA, 5367delA, 5368delA, 5369delG, 5370delC, 5371delG, 5372delA, 5373delG,
5374delC, 5375delA, 5376delA, 5377delG, 5378delA, 5379delG, 5380delA, 5381delA,
5382delT, 5383delC, 5384delC, 5385delC, 5386delA, 5387delG, 5388delG, 5389delA,
5390delC, 5391delA, 5392delG, 5393delA, 5394delA, 5395delA, 5396delG, 5397delA,
5398delT, 5399delC, 5400delT, 5401delT, 5402delC, 5403delA, 5404delG, 5405delG,
5406delG, 5407delG, 5408delG, 5409delC, 5410delT, 5411delA, 5412delG, 5413delA,
5414delA, 5415delA, 5416delT, 5417delC, 5418delT, 5419delG, 5420delT, 5421delT,
5422delG, 5423delC, 5424delT, 5425delA, 5426delT, 5427delG, 5428delG, 5429delG,
5430delC, 5431delC, 5432delC, 5433delT, 5434delT, 5435delC, 5436delA, 5437delC,
5438delC, 5439delA, 5440delA, 5441delC, 5442delA, 5443delT, 5444delG, 5445delC,
5446delC, 5447delC, 5448delA, 5449delC, 5450delA, 5451delG, 5452delA, 5453delT,
5454delC, 5455delA, 5456delA, 5457delC, 5458delT, 5459delG, 5460delG, 5461delA,
5462delA, 5463delT, 5464delG, 5465delG, 5466delA, 5467delT, 5468delG, 5469delG,
5470delT, 5471delA, 5472delC, 5473delA, 5474delG, 5475delC, 5476delT, 5477delG,
5478delT, 5479delG, 5480delT, 5481delG, 5482delG, 5483delT, 5484delG, 5485delC,
5486delT, 5487delT, 5488delC, 5489delT, 5490delG, 5491delT, 5492delG, 5493delG,
5494delT, 5495delG, 5496delA, 5497delA, 5498delG, 5499delG, 5500delA, 5501delG,
5502delC, 5503delT, 5504delT, 5505delT, 5506delC, 5507delA, 5508delT, 5509delC,
5510delA, 5511delT, 5512delT, 5513delC, 5514delA, 5515delC, 5516delC, 5517delC,
5518delT, 5519delT, 5520delG, 5521delG, 5522delC, 5523delA, 5524delC, 5525delA,
5526delG, 5527delG, 5528delT, 5529delG, 5530delT, 5531delC, 5532delC, 5533delA,
5534delC, 5535delC, 5536delC, 5537delA, 5538delA, 5539delT, 5540delT, 5541delG,
5542delT, 5543delG, 5544delG, 5545delT, 5546delT, 5547delG, 5548delT, 5549delG,
5550delC, 5551delA, 5552delG, 5553delC, 5554delC, 5555delA, 5556delG, 5557delA,
5558delT, 5559delG, 5560delC, 5561delC, 5562delT, 5563delG, 5564delG, 5565delA,
5566delC, 5567delA, 5568delG, 5569delA, 5570delG, 5571delG, 5572delA, 5573delC,
5574delA, 5575delA, 5576delT, 5577delG, 5578delG, 5579delC, 5580delT, 5581delT,
5582delC, 5583delC, 5584delA, 5585delT, 5586delG, 5587delC, 5588delA, 5589delA,
5590delT, 5591delT, 5592delG, 5593delG, 5594delG, 5595delC, 5596delA, 5597delG,
5598delA, 5599delT, 5600delG, 5601delT, 5602delG, 5603delT, 5604delG, 5605delA,
5606delG, 5607delG, 5608delC, 5609delA, 5610delC, 5611delC, 5612delT, 5613delG,
5614delT, 5615delG, 5616delG, 5617delT, 5618delG, 5619delA, 5620delC, 5621delC,
5622delC, 5623delG, 5624delA, 5625delG, 5626delA, 5627delG, 5628delT, 5629delG,
5630delG, 5631delG, 5632delT, 5633delG, 5634delT, 5635delT, 5636delG, 5637delG,
5638delA, 5639delC, 5640delA, 5641delG, 5642delT, 5643delG, 5644delT, 5645delA,
5646delG, 5647delC, 5648delA, 5649delC, 5650delT, 5651delC, 5652delT, 5653delA,
5654delC, 5655delC, 5656delA, 5657delG, 5658delT, 5659delG, 5660delC, 5661delC,
5662delA, 5663delG, 5664delG, 5665delA, 5666delG, 5667delC, 5668delT, 5669delG,
5670delG, 5671delA, 5672delC, 5673delA, 5674delC, 5675delC, 5676delT, 5677delA,
5678delC, 5679delC.

[0228]

TABLE 10
List of Two Base Deletions
4delGA, 5delAT, 6delTT, 7delTT, 8delTA, 9delAT, 10delTC, 11delCT, 12delTG, 132delGC,
133delCT, 134delTC, 135delCT, 136delTT, 137delTC, 138delCG, 139delGC, 140delCG,
141delGT, 142delTT, 143delTG, 144delGA, 145delAA, 146delAG, 147delGA, 148delAA,
149delAG, 150delGT, 151delTA, 152delAC, 153delCA, 154delAA, 155delAA, 156delAA,
157delAT, 158delTG, 159delGT, 160delTC, 161delCA, 162delAT, 163delTT, 164delTA,
165delAA, 166delAT, 167delTG, 168delGC, 169delCT, 170delTA, 171delAT, 172delTG,
173delGC, 174delCA, 175delAG, 176delGA, 177delAA, 178delAA, 179delAA, 180delAT,
181delTC, 182delCT, 183delTT, 184delTA, 185delAG, 186delGA, 187delAG, 188delGT,
189delTG, 190delGT, 191delTC, 192delCC, 193delCC, 194delCA, 195delAT, 196delTC,
197delCT, 198delTG, 199delGT, 200delTC, 201delCT, 202delTG, 203delGG, 204delGA,
205delAG, 206delGT, 207delTT, 208delTG, 209delGA, 210delAT, 211delTC, 212delCA,
213delAA, 214delAG, 215delGG, 216delGA, 217delAA, 218delAC, 219delCC, 220delCT,
221delTG, 222delGT, 223delTC, 224delCT, 225delTC, 226delCC, 227delCA, 228delAC,
229delCA, 230delAA, 231delAA, 232delAG, 233delGT, 234delTG, 235delGT, 236delTG,
237delGA, 238delAC, 239delCC, 240delCA, 241delAC, 242delCA, 243delAT, 244delTA,
245delAT, 246delTT, 247delTT, 248delTT, 249delTG, 250delGC, 251delCA, 252delAA,
253delAA, 254delAT, 255delTT, 256delTT, 257delTT, 258delTG, 259delGC, 260delCA,
261delAT, 262delTG, 263delGC, 264delCT, 265delTG, 266delGA, 267delAA, 268delAA,
269delAC, 270delCT, 271delTT, 272delTC, 273delCT, 274delTC, 275delCA, 276delAA,
277delAC, 278delCC, 279delCA, 280delAG, 281delGA, 282delAA, 283delAG, 284delGA,
285delAA, 286delAA, 287delAG, 288delGG, 289delGG, 290delGC, 291delCC, 292delCT,
293delTT, 294delTC, 295delCA, 296delAC, 297delCA, 298delAG, 299delGT, 300delTG,
301delGT, 302delTC, 303delCC, 304delCT, 305delTT, 306delTT, 307delTA, 308delAT,
309delTG, 310delGT, 311delTA, 312delAA, 313delAG, 314delGA, 315delAA, 316delAT,
317delTG, 318delGA, 319delAT, 320delTA, 321delAT, 322delTA, 323delAA, 324delAC,
325delCC, 326delCA, 327delAA, 328delAA, 329delAA, 330delAG, 331delGG, 332delGA,
333delAG, 334delGC, 335delCC, 336delCT, 337delTA, 338delAC, 339delCA, 340delAA,
341delAG, 342delGA, 343delAA, 344delAA, 345delAG, 346delGT, 347delTA, 348delAC,
349delCG, 350delGA, 351delAG, 352delGA, 353delAT, 354delTT, 355delTT, 356delTA,
357delAG, 358delGT, 359delTC, 360delCA, 361delAA, 362delAC, 363delCT, 364delTT,
365delTG, 366delGT, 367delTT, 368delTG, 369delGA, 370delAA, 371delAG, 372delGA,
373delAG, 374delGC, 375delCT, 376delTA, 377delAT, 378delTT, 379delTG, 380delGA,
381delAA, 382delAA, 383delAA, 384delAT, 385delTC, 386delCA, 387delAT, 388delTT,
389delTT, 390delTG, 391delGT, 392delTG, 393delGC, 394delCT, 395delTT, 396delTT,
397delTT, 398delTC, 399delCA, 400delAG, 401delGC, 402delCT, 403delTT, 404delTG,
405delGA, 406delAC, 407delCA, 408delAC, 409delCA, 410delAG, 411delGG, 412delGT,
413delTT, 414delTT, 415delTG, 416delGG, 417delGA, 418delAG, 419delGT, 420delTA,
421delAT, 422delTG, 423delGC, 424delCA, 425delAA, 426delAA, 427delAC, 428delCA,
429delAG, 430delGC, 431delCT, 432delTA, 433delAT, 434delTA, 435delAA, 436delAT,
437delTT, 438delTT, 439delTT, 440delTG, 441delGC, 442delCA, 443delAA, 444delAA,
445delAA, 446delAA, 447delAA, 448delAG, 449delGG, 450delGA, 451delAA, 452delAA,
453delAA, 454delAT, 455delTA, 456delAA, 457delAC, 458delCT, 459delTC, 460delCT,
461delTC, 462delCC, 463delCT, 464delTG, 465delGA, 466delAA, 467delAC, 468delCA,
469delAT, 470delTC, 471delCT, 472delTA, 473delAA, 474delAA, 475delAA, 476delAG,
477delGA, 478delAT, 479delTG, 480delGA, 481delAA, 482delAG, 483delGT, 484delTT,
485delTT, 486delTC, 487delCT, 488delTA, 489delAT, 490delTC, 491delCA, 492delAT,
493delTC, 494delCC, 495delCA, 496delAA, 497delAA, 498delAG, 499delGT, 500delTA,
501delAT, 502delTG, 503delGG, 504delGG, 505delGC, 506delCT, 507delTA, 508delAC,
509delCA, 510delAG, 511delGA, 512delAA, 513delAA, 514delAC, 515delCC, 516delCG,
517delGT, 518delTG, 519delGC, 520delCC, 521delCA, 522delAA, 523delAA, 524delAA,
525delAG, 526delGA, 527delAC, 528delCT, 529delTT, 530delTC, 531delCT, 532delTA,
533delAC, 534delCA, 535delAG, 536delGA, 537delAG, 538delGT, 539delTG, 540delGA,
541delAA, 542delAC, 543delCC, 544delCC, 545delCG, 546delGA, 547delAA, 548delAA,
549delAA, 550delAT, 551delTC, 552delCC, 553delCT, 554delTT, 555delTC, 556delCC,
557delCT, 558delTT, 559delTG, 560delGC, 561delCA, 562delAG, 563delGG, 564delGA,
565delAA, 566delAA, 567delAC, 568delCC, 569delCA, 570delAG, 571delGT, 572delTC,
573delCT, 574delTC, 575delCA, 576delAG, 577delGT, 578delTG, 579delGT, 580delTC,
581delCC, 582delCA, 583delAA, 584delAC, 585delCT, 586delTC, 587delCT, 588delTC,
589delCT, 590delTA, 591delAA, 592delAC, 593delCC, 594delCT, 595delTT, 596delTG,
597delGG, 598delGA, 599delAA, 600delAC, 601delCT, 602delTG, 603delGT, 604delTG,
605delGA, 606delAG, 607delGA, 608delAA, 609delAC, 610delCT, 611delTC, 612delCT,
613delTG, 614delGA, 615delAG, 616delGG, 617delGA, 618delAC, 619delCA, 620delAA,
621delAA, 622delAG, 623delGC, 624delCA, 625delAG, 626delGC, 627delCG, 628delGG,
629delGA, 630delAT, 631delTA, 632delAC, 633delCA, 634delAA, 635delAC, 636delCC,
637delCT, 638delTC, 639delCA, 640delAA, 641delAA, 642delAA, 643delAG, 644delGA,
645delAC, 646delCG, 647delGT, 648delTC, 649delCT, 650delTG, 651delGT, 652delTC,
653delCT, 654delTA, 655delAC, 656delCA, 657delAT, 658delTT, 659delTG, 660delGA,
661delAA, 662delAT, 663delTT, 664delTG, 665delGG, 666delGG, 667delGA, 668delAT,
669delTC, 670delCT, 671delTG, 672delGA, 673delAT, 674delTT, 675delTC, 676delCT,
677delTT, 678delTC, 679delCT, 680delTG, 681delGA, 682delAA, 683delAG, 684delGA,
685delAT, 686delTA, 687delAC, 688delCC, 689delCG, 690delGT, 691delTT, 692delTA,
693delAA, 694delAT, 695delTA, 696delAA, 697delAG, 698delGG, 699delGC, 700delCA,
701delAA, 702delAC, 703delCT, 704delTT, 705delTA, 706delAT, 707delTT, 708delTG,
709delGC, 710delCA, 711delAG, 712delGT, 713delTG, 714delGT, 715delTG, 716delGG,
717delGG, 718delGA, 719delAG, 720delGA, 721delAT, 722delTC, 723delCA, 724delAA,
725delAG, 726delGA, 727delAA, 728delAT, 729delTT, 730delTG, 731delGT, 732delTT,
733delTA, 734delAC, 735delCA, 736delAA, 737delAA, 738delAT, 739delTC, 740delCA,
741delAC, 742delCC, 743delCC, 744delCC, 745delCT, 746delTC, 747delCA, 748delAA,
749delAG, 750delGG, 751delGA, 752delAA, 753delAC, 754delCC, 755delCA, 756delAG,
757delGG, 758delGG, 759delGA, 760delAT, 761delTG, 762delGA, 763delAA, 764delAA,
765delAT, 766delTC, 767delCA, 768delAG, 769delGT, 770delTT, 771delTT, 772delTG,
773delGG, 774delGA, 775delAT, 776delTT, 777delTC, 778delCT, 779delTG, 780delGC,
781delCA, 782delAA, 783delAA, 784delAA, 785delAA, 786delAA, 787delAG, 788delGG,
789delGC, 790delCT, 791delTG, 792delGC, 793delCT, 794delTT, 795delTG, 796delGT,
797delTG, 798delGA, 799delAA, 800delAT, 801delTT, 802delTT, 803delTT, 804delTC,
805delCT, 806delTG, 807delGA, 808delAG, 809delGA, 810delAC, 811delCG, 812delGG,
813delGA, 814delAT, 815delTG, 816delGT, 817delTA, 818delAA, 819delAC, 820delCA,
821delAA, 822delAA, 823delAT, 824delTA, 825delAC, 826delCT, 827delTG, 828delGA,
829delAA, 830delAC, 831delCA, 832delAT, 833delTC, 834delCA, 835delAT, 836delTC,
837delCA, 838delAA, 839delAC, 840delCC, 841delCC, 842delCA, 843delAG, 844delGT,
845delTA, 846delAA, 847delAT, 848delTA, 849delAA, 850delAT, 851delTG, 852delGA,
853delAT, 854delTT, 855delTT, 856delTG, 857delGA, 858delAA, 859delAC, 860delCA,
861delAC, 862delCC, 863delCA, 864delAC, 865delCT, 866delTG, 867delGA, 868delAG,
869delGA, 870delAA, 871delAG, 872delGC, 873delCG, 874delGT, 875delTG, 876delGC,
877delCA, 878delAG, 879delGC, 880delCT, 881delTG, 882delGA, 883delAG, 884delGA,
885delAG, 886delGG, 887delGC, 888delCA, 889delAT, 890delTC, 891delCC, 892delCA,
893delAG, 894delGA, 895delAA, 896delAA, 897delAA, 898delAG, 899delGT, 900delTA,
901delAT, 902delTC, 903delCA, 904delAG, 905delGG, 906delGG, 907delGT, 908delTA,
909delAG, 910delGT, 911delTT, 912delTC, 913delCT, 914delTG, 915delGT, 916delTT,
917delTT, 918delTC, 919delCA, 920delAA, 921delAA, 922delAC, 923delCT, 924delTT,
925delTG, 926delGC, 927delCA, 928delAT, 929delTG, 930delGT, 931delTG, 932delGG,
933delGA, 934delAG, 935delGC, 936delCC, 937delCA, 938delAT, 939delTG, 940delGT,
941delTG, 942delGG, 943delGC, 944delCA, 945delAC, 946delCA, 947delAA, 948delAA,
949delAT, 950delTA, 951delAC, 952delCT, 953delTC, 954delCA, 955delAT, 956delTG,
957delGC, 958delCC, 959delCA, 960delAG, 961delGC, 962delCT, 963delTC, 964delCA,
965delAT, 966delTT, 967delTA, 968delAC, 969delCA, 970delAG, 971delGC, 972delCA,
973delAT, 974delTG, 975delGA, 976delAG, 977delGA, 978delAA, 979delAC, 980delCA,
981delAG, 982delGC, 983delCA, 984delAG, 985delGT, 986delTT, 987delTT, 988delTA,
989delAT, 990delTT, 991delTA, 992delAC, 993delCT, 994delTC, 995delCA, 996delAC,
997delCT, 998delTA, 999delAA, 1000delAA, 1001delAG, 1002delGA, 1003delAC,
1004delCA, 1005delAG, 1006delGA, 1007delAA, 1008delAT, 1009delTG, 1010delGA,
1011delAA, 1012delAT, 1013delTG, 1014delGT, 1015delTA, 1016delAG, 1017delGA,
1018delAA, 1019delAA, 1020delAA, 1021delAG, 1022delGG, 1023delGC, 1024delCT,
1025delTG, 1026delGA, 1027delAA, 1028delAT, 1029delTT, 1030delTC, 1031delCT,
1032delTG, 1033delGT, 1034delTA, 1035delAA, 1036delAT, 1037delTA, 1038delAA,
1039delAA, 1040delAA, 1041delAG, 1042delGC, 1043delCA, 1044delAA, 1045delAA,
1046delAC, 1047delCA, 1048delAG, 1049delGC, 1050delCC, 1051delCT, 1052delTG,
1053delGG, 1054delGC, 1055delCT, 1056delTT, 1057delTA, 1058delAG, 1059delGC,
1060delCA, 1061delAA, 1062delAG, 1063delGG, 1064delGA, 1065delAG, 1066delGC,
1067delCC, 1068delCA, 1069delAA, 1070delAC, 1071delCA, 1072delAT, 1073delTA,
1074delAA, 1075delAC, 1076delCA, 1077delAG, 1078delGA, 1079delAT, 1080delTG,
1081delGG, 1082delGG, 1083delGC, 1084delCT, 1085delTG, 1086delGG, 1087delGA,
1088delAA, 1089delAG, 1090delGT, 1091delTA, 1092delAA, 1093delAG, 1094delGG,
1095delGA, 1096delAA, 1097delAA, 1098delAC, 1099delCA, 1100delAT, 1101delTG,
1102delGT, 1103delTA, 1104delAA, 1105delAT, 1106delTG, 1107delGA, 1108delAT,
1109delTA, 1110delAG, 1111delGG, 1112delGC, 1113delCG, 1114delGG, 1115delGA,
1116delAC, 1117delCT, 1118delTC, 1119delCC, 1120delCC, 1121delCA, 1122delAG,
1123delGC, 1124delCA, 1125delAC, 1126delCA, 1127delAG, 1128delGA, 1129delAA,
1130delAA, 1131delAA, 1132delAA, 1133delAA, 1134delAA, 1135delAG, 1136delGG,
1137delGT, 1138delTA, 1139delAG, 1140delGA, 1141delAT, 1142delTC, 1143delCT,
1144delTG, 1145delGA, 1146delAA, 1147delAT, 1148delTG, 1149delGC, 1150delCT,
1151delTG, 1152delGA, 1153delAT, 1154delTC, 1155delCC, 1156delCC, 1157delCC,
1158delCT, 1159delTG, 1160delGT, 1161delTG, 1162delGT, 1163delTG, 1164delGA,
1165delAG, 1166delGA, 1167delAG, 1168delGA, 1169delAA, 1170delAA, 1171delAA,
1172delAG, 1173delGA, 1174delAA, 1175delAT, 1176delTG, 1177delGG, 1178delGA,
1179delAA, 1180delAT, 1181delTA, 1182delAA, 1183delAG, 1184delGC, 1185delCA,
1186delAG, 1187delGA, 1188delAA, 1189delAA, 1190delAC, 1191delCT, 1192delTG,
1193delGC, 1194delCC, 1195delCA, 1196delAT, 1197delTG, 1198delGC, 1199delCT,
1200delTC, 1201delCA, 1202delAG, 1203delGA, 1204delAG, 1205delGA, 1206delAA,
1207delAT, 1208delTC, 1209delCC, 1210delCT, 1211delTA, 1212delAG, 1213delGA,
1214delAG, 1215delGA, 1216delAT, 1217delTA, 1218delAC, 1219delCT, 1220delTG,
1221delGA, 1222delAA, 1223delAG, 1224delGA, 1225delAT, 1226delTG, 1227delGT,
1228delTT, 1229delTC, 1230delCC, 1331delCT, 1232delTT, 1233delTG, 1234delGG,
1235delGA, 1236delAT, 1237delTA, 1238delAA, 1239delAC, 1240delCA, 1241delAC,
1242delCT, 1243delTA, 1244delAA, 1245delAA, 1246delAT, 1247delTA, 1248delAG,
1249delGC, 1250delCA, 1251delAG, 1252delGC, 1253delCA, 1254delAT, 1255delTT,
1256delTC, 1257delCA, 1258delAG, 1259delGA, 1260delAA, 1261delAA, 1262delAG,
1263delGT, 1264delTT, 1265delTA, 1266delAA, 1267delAT, 1268delTG, 1269delGA,
1270delAG, 1271delGT, 1272delTG, 1273delGG, 1274delGT, 1275delTT, 1276delTT,
1277delTT, 1278delTC, 1279delCC, 1280delCA, 1281delAG, 1282delGA, 1283delAA,
1284delAG, 1285delGT, 1286delTG, 1287delGA, 1288delAT, 1289delTG, 1290delGA,
1291delAA, 1292delAC, 1293delCT, 1294delTG, 1295delGT, 1296delTT, 1297delTA,
1298delAG, 1299delGG, 1300delGT, 1301delTT, 1302delTC, 1303delCT, 1304delTG,
1305delGA, 1306delAT, 1307delTG, 1308delGA, 1309delAC, 1310delCT, 1311delTC,
1312delCA, 1313delAC, 1314delCA, 1315delAT, 1316delTG, 1317delGA, 1318delAT,
1319delTG, 1320delGG, 1321delGG, 1322delGG, 1323delGA, 1324delAG, 1325delGT,
1326delTC, 1327delCT, 1328delTG, 1329delGA, 1330delAA, 1331delAT, 1332delTC,
1333delCA, 1334delAA, 1335delAA, 1336delAT, 1337delTG, 1338delGC, 1339delCC,
1340delCA, 1341delAA, 1342delAA, 1343delAG, 1344delGT, 1345delTA, 1346delAG,
1347delGC, 1348delCT, 1349delTG, 1350delGA, 1351delAT, 1352delTG, 1353delGT,
1354delTA, 1355delAT, 1356delTT, 1357delTG, 1358delGG, 1359delGA, 1360delAC,
1361delCG, 1362delGT, 1363delTT, 1364delTC, 1365delCT, 1366delTA, 1367delAA,
1368delAA, 1369delAT, 1370delTG, 1371delGA, 1372delAG, 1373delGG, 1374delGT,
1375delTA, 1376delAG, 1377delGA, 1378delAT, 1379delTG, 1380delGA, 1381delAA,
1382delAT, 1383delTA, 1384delAT, 1385delTT, 1386delTC, 1387delCT, 1388delTG,
1389delGG, 1390delGT, 1391delTT, 1392delTC, 1393delCT, 1394delTT, 1395delTC,
1396delCA, 1397delAG, 1398delGA, 1399delAG, 1400delGA, 1401delAA, 1402delAA,
1403delAA, 1404delAT, 1405delTA, 1406delAG, 1407delGA, 1408delAC, 1409delCT,
1410delTT, 1411delTA, 1412delAC, 1413delCT, 1414delTG, 1415delGG, 1416delGC,
1417delCC, 1418delCA, 1419delAG, 1420delGT, 1421delTG, 1422delGA, 1423delAT,
1424delTC, 1425delCC, 1426delCT, 1427delTC, 1428delCA, 1429delAT, 1430delTG,
1431delGA, 1432delAG, 1433delGG, 1434delGC, 1435delCT, 1436delTT, 1437delTT,
1438delTA, 1439delAA, 1440delAT, 1441delTA, 1442delAT, 1443delTG, 1444delGT,
1445delTA, 1446delAA, 1447delAA, 1448delAA, 1449delAG, 1450delGT, 1451delTG,
1452delGA, 1453delAA, 1454delAA, 1455delAG, 1456delGA, 1457delAG, 1458delGT,
1459delTT, 1460delTC, 1461delCA, 1462delAC, 1463delCT, 1464delTC, 1465delCC,
1466delCA, 1467delAA, 1468delAA, 1469delAT, 1470delTC, 1471delCA, 1472delAG,
1473delGT, 1474delTA, 1475delAG, 1476delGA, 1477delAG, 1478delGA, 1479delAG,
1480delGT, 1481delTA, 1482delAA, 1483delAT, 1484delTA, 1485delAT, 1486delTT,
1487delTG, 1488delGA, 1489delAA, 1490delAG, 1491delGA, 1492delAC, 1493delCA,
1494delAA, 1495delAA, 1496delAA, 1497delAT, 1498delTA, 1499delAT, 1500delTT,
1501delTT, 1502delTG, 1503delGG, 1504delGG, 1505delGA, 1506delAA, 1507delAA,
1508delAA, 1509delAC, 1510delCC, 1511delCT, 1512delTA, 1513delAT, 1514delTC,
1515delCG, 1516delGG, 1517delGA, 1518delAA, 1519delAG, 1520delGA, 1521delAA,
1522delAG, 1523delGG, 1524delGC, 1525delCA, 1526delAA, 1527delAG, 1528delGC,
1529delCC, 1530delCT, 1531delTC, 1532delCC, 1533delCC, 1534delCC, 1535delCA,
1536delAA, 1537delAC, 1538delCT, 1539delTT, 1540delTA, 1541delAA, 1542delAG,
1543delGC, 1544delCC, 1545delCA, 1546delAT, 1547delTG, 1548delGT, 1549delTA,
1550delAA, 1551delAC, 1552delCT, 1553delTG, 1554delGA, 1555delAA, 1556delAA,
1557delAA, 1558delAT, 1559delTC, 1560delCT, 1561delTA, 1562delAA, 1563delAT,
1564delTT, 1565delTA, 1566delAT, 1567delTA, 1568delAG, 1569delGG, 1570delGA,
1571delAG, 1572delGC, 1573delCA, 1574delAT, 1575delTT, 1576delTT, 1577delTG,
1578delGT, 1579delTT, 1580delTA, 1581delAC, 1582delCT, 1583delTG, 1584delGA,
1585delAG, 1586delGC, 1587delCC, 1588delCA, 1589delAC, 1590delCA, 1591delAG,
1592delGA, 1593delAT, 1594delTA, 1595delAA, 1596delAT, 1597delTA, 1598delAC,
1599delCA, 1600delAA, 1601delAG, 1602delGA, 1603delAG, 1604delGC, 1605delCG,
1606delGT, 1607delTC, 1608delCC, 1609delCC, 1610delCC, 1611delCT, 1612delTC,
1613delCA, 1614delAC, 1615delCA, 1616delAA, 1617delAA, 1618delAT, 1619delTA,
1620delAA, 1621delAA, 1622delAT, 1623delTT, 1624delTA, 1625delAA, 1626delAA,
1627delAG, 1628delGC, 1629delCG, 1630delGT, 1631delTA, 1632delAA, 1633delAA,
1634delAA, 1635delAG, 1636delGG, 1637delGA, 1638delAG, 1639delGA, 1640delAC,
1641delCC, 1642delCT, 1643delTA, 1644delAC, 1645delCA, 1646delAT, 1647delTC,
1648delCA, 1649delAG, 1650delGG, 1651delGC, 1652delCC, 1653delCT, 1654delTT,
1655delTC, 1656delCA, 1657delAT, 1658delTC, 1659delCC, 1660delCT, 1661delTG,
1662delGA, 1663delAG, 1664delGG, 1665delGA, 1666delAT, 1667delTT, 1668delTT,
1669delTT, 1670delTA, 1671delAT, 1672delTC, 1673delCA, 1674delAA, 1675delAG,
1676delGA, 1677delAA, 1678delAA, 1679delAG, 1680delGC, 1681delCA, 1682delAG,
1683delGA, 1684delAT, 1685delTT, 1686delTT, 1687delTG, 1688delGG, 1689delGC,
1690delCA, 1691delAG, 1692delGT, 1693delTT, 1694delTC, 1695delCA, 1696delAA,
1697delAA, 1698delAA, 1699delAG, 1700delGA, 1701delAC, 1702delCT, 1703delTC,
1704delCC, 1705delCT, 1706delTG, 1707delGA, 1708delAA, 1709delAA, 1710delAT,
1711delTG, 1712delGA, 1713delAT, 1714delTA, 1715delAA, 1716delAA, 1717delAT,
1718delTC, 1719delCA, 1720delAG, 1721delGG, 1722delGG, 1723delGA, 1724delAA,
1725delAC, 1726delCT, 1727delTA, 1728delAA, 1729delAC, 1730delCC, 1731delCA,
1732delAA, 1733delAA, 1734delAC, 1735delCG, 1736delGG, 1737delGA, 1738delAG,
1739delGC, 1740delCA, 1741delAG, 1742delGA, 1743delAA, 1744delAT, 1745delTG,
1746delGG, 1747delGT, 1748delTC, 1749delCA, 1750delAA, 1751delAG, 1752delGT,
1753delTG, 1754delGA, 1755delAT, 1756delTG, 1757delGA, 1758delAA, 1759delAT,
1760delTA, 1761delAT, 1762delTT, 1763delTA, 1764delAC, 1765delCT, 1766delTA,
1767delAA, 1768delAT, 1769delTA, 1770delAG, 1771delGT, 1772delTG, 1773delGG,
1774delGT, 1775delTC, 1776delCA, 1777delAT, 1778delTG, 1779delGA, 1780delAG,
1781delGA, 1782delAA, 1783delAT, 1784delTA, 1785delAA, 1786delAA, 1787delAA,
1788delAC, 1789delCA, 1790delAA, 1791delAA, 1792delAA, 1793delAG, 1794delGG,
1795delGT, 1796delTG, 1797delGA, 1798delAT, 1799delTT, 1800delTC, 1801delCT,
1802delTA, 1803delAT, 1804delTT, 1805delTC, 1806delCA, 1807delAG, 1808delGA,
1809delAA, 1810delAT, 1811delTG, 1812delGA, 1813delAG, 1814delGA, 1815delAA,
1816delAA, 1817delAA, 1818delAA, 1819delAT, 1820delTC, 1821delCC, 1822delCT,
1823delTA, 1824delAA, 1825delAC, 1826delCC, 1827delCC, 1828delCA, 1829delAA,
1830delAT, 1831delTA, 1832delAG, 1833delGA, 1834delAA, 1835delAT, 1836delTC,
1837delCA, 1838delAC, 1839delCT, 1840delTC, 1841delCG, 1842delGA, 1843delAA,
1844delAA, 1845delAA, 1846delAA, 1847delAG, 1848delGA, 1849delAA, 1850delAT,
1851delTC, 1852delCT, 1853delTG, 1854delGC, 1855delCT, 1856delTT, 1857delTT,
1858delTC, 1859delCA, 1860delAA, 1861delAA, 1862delAA, 1863delAC, 1864delCG,
1865delGA, 1866delAA, 1867delAA, 1868delAG, 1869delGC, 1870delCT, 1871delTG,
1872delGA, 1873delAA, 1874delAC, 1875delCC, 1876delCT, 1877delTA, 1878delAT,
1879delTA, 1880delAA, 1881delAG, 1882delGC, 1883delCA, 1884delAG, 1885delGC,
1886delCA, 1887delAG, 1888delGT, 1889delTA, 1890delAT, 1891delTA, 1892delAA,
1893delAG, 1894delGC, 1895delCA, 1896delAA, 1897delAT, 1898delTA, 1899delAT,
1900delTG, 1901delGG, 1902delGA, 1903delAA, 1904delAC, 1905delCT, 1906delTC,
1907delCG, 1908delGA, 1909delAA, 1910delAT, 1911delTT, 1912delTA, 1913delAA,
1914delAA, 1915delAT, 1916delTA, 1917delAT, 1918delTC, 1919delCC, 1920delCA,
1921delAC, 1922delCA, 1923delAA, 1924delAT, 1925delTT, 1926delTC, 1927delCA,
1928delAA, 1929delAA, 1930delAA, 1931delAG, 1932delGC, 1933delCA, 1934delAC,
1935delCC, 1936delCT, 1937delTA, 1938delAA, 1939delAA, 1940delAA, 1941delAA,
1942delAG, 1943delGA, 1944delAA, 1945delAT, 1946delTA, 1947delAG, 1948delGG,
1949delGC, 1950delCT, 1951delTG, 1952delGA, 1953delAG, 1954delGG, 1955delGA,
1956delAG, 1957delGG, 1958delGA, 1959delAA, 1960delAG, 1961delGT, 1962delTC,
1963delCT, 1964delTT, 1965delTC, 1966delCT, 1967delTA, 1968delAC, 1969delCC,
1970delCA, 1971delAG, 1972delGG, 1973delGC, 1974delCA, 1975delAT, 1976delTA,
1977delAT, 1978delTT, 1979delTC, 1980delCA, 1981delAT, 1982delTG, 1983delGC,
1984delCG, 1985delGC, 1986delCT, 1987delTT, 1988delTG, 1989delGA, 1990delAA,
1991delAC, 1992delCT, 1993delTA, 1994delAG, 1995delGT, 1996delTA, 1997delAG,
1998delGT, 1999delTC, 2000delCA, 2001delAG, 2002delGT, 2003delTA, 2004delAG,
2005delGA, 2006delAA, 2007delAA, 2008delAT, 2009delTC, 2010delCT, 2011delTA,
2012delAA, 2013delAG, 2014delGC, 2015delCC, 2016delCC, 2017delCA, 2018delAC,
2019delCC, 2020delCT, 2021delTA, 2022delAA, 2023delAT, 2024delTT, 2025delTG,
2026delGT, 2027delTA, 2028delAC, 2029delCT, 2030delTG, 2031delGA, 2032delAA,
2033delAT, 2034delTT, 2035delTG, 2036delGC, 2037delCA, 2038delAA, 2039delAA,
2040delAT, 2041delTT, 2042delTG, 2043delGA, 2044delAT, 2045delTA, 2046delAG,
2047delGT, 2048delTT, 2049delTG, 2050delGT, 2051delTT, 2052delTC, 2053delCT,
2054delTA, 2055delAG, 2056delGC, 2057delCA, 2058delAG, 2059delGT, 2060delTG,
2061delGA, 2062delAA, 2063delAG, 2064delGA, 2065delAG, 2066delGA, 2067delAT,
2068delTA, 2069delAA, 2070delAA, 2071delAG, 2072delGA, 2073delAA, 2074delAA,
2075delAA, 2076delAA, 2077delAA, 2078delAA, 2079delAA, 2080delAG, 2081delGT,
2082delTA, 2083delAC, 2084delCA, 2085delAA, 2086delAC, 2087delCC, 2088delCA,
2089delAA, 2090delAA, 2091delAT, 2092delTG, 2093delGC, 2094delCC, 2095delCA,
2096delAG, 2097delGT, 2098delTC, 2099delCA, 2100delAG, 2101delGG, 2102delGC,
2103delCA, 2104delAC, 2105delCA, 2106delAG, 2107delGC, 2108delCA, 2109delAG,
2110delGA, 2111delAA, 2112delAA, 2113delAC, 2114delCC, 2115delCT, 2116delTA,
2117delAC, 2118delCA, 2119delAA, 2120delAC, 2121delCT, 2122delTC, 2123delCA,
2124delAT, 2125delTG, 2126delGG, 2127delGA, 2128delAA, 2129delAG, 2130delGG,
2131delGT, 2132delTA, 2133delAA, 2134delAA, 2135delAG, 2136delGA, 2137delAA,
2138delAC, 2139delCC, 2140delCT, 2141delTG, 2142delGC, 2143delCA, 2144delAA,
2145delAC, 2146delCT, 2147delTG, 2148delGG, 2149delGA, 2150delAG, 2151delGC,
2152delCC, 2153delCA, 2154delAA, 2155delAG, 2156delGA, 2157delAA, 2158delAG,
2159delGA, 2160delAG, 2161delGT, 2162delTA, 2163delAA, 2164delAC, 2165delCA,
2166delAA, 2167delAG, 2168delGC, 2169delCC, 2170delCA, 2171delAA, 2172delAA,
2173delAT, 2174delTG, 2175delGA, 2176delAA, 2177delAC, 2178delCA, 2179delAG,
2180delGA, 2181delAC, 2182delCA, 2183delAA, 2184delAG, 2185delGT, 2186delTA,
2187delAA, 2188delAA, 2189delAA, 2190delAG, 2191delGA, 2192delAC, 2193delCA,
2194delAT, 2195delTG, 2196delGA, 2197delAC, 2198delCA, 2199delAG, 2200delGT,
2201delTG, 2202delGA, 2203delAT, 2204delTA, 2205delAC, 2206delCT, 2207delTT,
2208delTT, 2209delTC, 2210delCC, 2211delCC, 2212delCA, 2213delAG, 2214delGA,
2215delAG, 2216delGC, 2217delCT, 2218delTG, 2219delGA, 2220delAA, 2221delAG,
2222delGT, 2223delTT, 2224delTA, 2225delAA, 2226delAC, 2227delCA, 2228delAA,
2229delAA, 2230delAT, 2231delTG, 2232delGC, 2233delCA, 2234delAC, 2235delCC,
2236delCT, 2237delTG, 2238delGG, 2239delGT, 2240delTT, 2241delTC, 2242delCT,
2243delTT, 2244delTT, 2245delTT, 2246delTA, 2247delAC, 2248delCT, 2249delTA,
2250delAA, 2251delAG, 2252delGT, 2253delTG, 2254delGT, 2255delTT, 2256delTC,
2257delCA, 2258delAA, 2259delAA, 2260delAT, 2261delTA, 2262delAC, 2263delCC,
2264delCA, 2265delAG, 2266delGT, 2267delTG, 2268delGA, 2269delAA, 2270delAC,
2271delCT, 2272delTT, 2273delTA, 2274delAA, 2275delAA, 2276delAG, 2277delGA,
2278delAA, 2279delAT, 2280delTT, 2281delTT, 2282delTG, 2283delGT, 2284delTC,
2285delCA, 2286delAA, 2287delAT, 2288delTC, 2289delCC, 2290delCT, 2291delTA,
2292delAG, 2293delGC, 2294delCC, 2295delCT, 2296delTT, 2297delTC, 2298delCC,
2299delCA, 2300delAA, 2301delAG, 2302delGA, 2303delAG, 2304delGA, 2305delAA,
2306delAG, 2307delGA, 2308delAA, 2309delAA, 2310delAA, 2311delAA, 2312delAG,
2313delGA, 2314delAA, 2315delAG, 2316delGA, 2317delAG, 2318delGA, 2319delAA,
2320delAA, 2321delAC, 2322delCT, 2323delTA, 2324delAG, 2325delGA, 2326delAA,
2327delAA, 2328delAC, 2329delCA, 2330delAG, 2331delGT, 2332delTT, 2333delTA,
2334delAA, 2335delAA, 2336delAG, 2337delGT, 2338delTG, 2339delGT, 2340delTC,
2341delCT, 2342delTA, 2343delAA, 2344delAT, 2345delTA, 2346delAA, 2347delAT,
2348delTG, 2349delGC, 2350delCT, 2351delTG, 2352delGA, 2353delAA, 2354delAG,
2355delGA, 2356delAC, 2357delCC, 2358delCC, 2359delCC, 2360delCA, 2361delAA,
2362delAA, 2363delAG, 2364delGA, 2365delAT, 2366delTC, 2367delCT, 2368delTC,
2369delCA, 2370delAT, 2371delTG, 2372delGT, 2373delTT, 2374delTA, 2375delAA,
2376delAG, 2377delGT, 2378delTG, 2379delGG, 2380delGA, 2381delAG, 2382delGA,
2383delAA, 2384delAA, 2385delAG, 2386delGG, 2387delGG, 2388delGT, 2389delTT,
2390delTT, 2391delTT, 2392delTG, 2393delGC, 2394delCA, 2395delAA, 2396delAA,
2397delAC, 2398delCT, 2399delTG, 2400delGA, 2401delAA, 2402delAA, 2403delAG,
2404delGA, 2405delAT, 2406delTC, 2407delCT, 2408delTG, 2409delGT, 2410delTA,
2411delAG, 2412delGA, 2413delAG, 2414delGA, 2415delAG, 2416delGT, 2417delTA,
2418delAG, 2419delGC, 2420delCA, 2421delAG, 2422delGT, 2423delTA, 2424delAT,
2425delTT, 2426delTT, 2427delTC, 2428delCA, 2429delAC, 2430delCT, 2431delTG,
2432delGG, 2433delGT, 2434delTA, 2435delAC, 2436delCC, 2437delCT, 2438delTG,
2439delGG, 2440delGT, 2441delTA, 2442delAC, 2443delCT, 2444delTG, 2445delGA,
2446delAT, 2447delTT, 2448delTA, 2449delAT, 2450delTG, 2451delGG, 2452delGC,
2453delCA, 2454delAC, 2455delCT, 2456delTC, 2457delCA, 2458delAG, 2459delGG,
2460delGA, 2461delAA, 2462delAA, 2463delAG, 2464delGT, 2465delTA, 2466delAT,
2467delTC, 2468delCT, 2469delTC, 2470delCG, 2471delGT, 2472delTT, 2473delTA,
2474delAC, 2475delCT, 2476delTG, 2477delGG, 2478delGA, 2479delAA, 2480delAG,
2481delGT, 2482delTT, 2483delTA, 2484delAG, 2485delGC, 2486delCA, 2487delAC,
2488delCT, 2489delTC, 2490delCT, 2491delTA, 2492delAG, 2493delGG, 2494delGG,
2495delGA, 2496delAA, 2497delAG, 2498delGG, 2499delGC, 2500delCA, 2501delAA,
2502delAA, 2503delAA, 2504delAA, 2505delAC, 2506delCA, 2507delAG, 2508delGA,
2509delAA, 2510delAC, 2511delCC, 2512delCA, 2513delAA, 2514delAA, 2515delAT,
2516delTA, 2517delAA, 2518delAA, 2519delAT, 2520delTG, 2521delGT, 2522delTG,
2523delGT, 2524delTG, 2525delGA, 2526delAG, 2527delGT, 2528delTC, 2529delCA,
2530delAG, 2531delGT, 2532delTG, 2533delGT, 2534delTG, 2535delGC, 2536delCA,
2537delAG, 2538delGC, 2539delCA, 2540delAT, 2541delTT, 2542delTT, 2543delTG,
2544delGA, 2545delAA, 2546delAA, 2547delAA, 2548delAC, 2549delCC, 2550delCC,
2551delCC, 2552delCA, 2553delAA, 2554delAG, 2555delGG, 2556delGG, 2557delGA,
2558delAC, 2559delCT, 2560delTA, 2561delAA, 2562delAT, 2563delTT, 2564delTC,
2565delCA, 2566delAT, 2567delTG, 2568delGG, 2569delGT, 2570delTT, 2571delTG,
2572delGT, 2573delTT, 2574delTC, 2575delCC, 2576delCA, 2577delAA, 2578delAA,
2579delAG, 2580delGA, 2581delAT, 2582delTA, 2583delAA, 2584delAT, 2585delTA,
2586delAG, 2587delGA, 2588delAA, 2589delAA, 2590delAT, 2591delTG, 2592delGA,
2593delAC, 2594delCA, 2595delAC, 2596delCA, 2597delAG, 2598delGA, 2599delAA,
2600delAG, 2601delGG, 2602delGC, 2603delCT, 2604delTT, 2605delTT, 2606delTA,
2607delAA, 2608delAG, 2609delGT, 2610delTA, 2611delAT, 2612delTC, 2613delCC,
2614delCA, 2615delAT, 2616delTT, 2617delTG, 2618delGG, 2619delGG, 2620delGA,
2621delAC, 2622delCA, 2623delAT, 2624delTG, 2625delGA, 2626delAA, 2627delAG,
2628delGT, 2629delTT, 2630delTA, 2631delAA, 2632delAC, 2633delCC, 2634delCA,
2635delAC, 2636delCA, 2637delAG, 2638delGT, 2639delTC, 2640delCG, 2641delGG,
2642delGG, 2643delGA, 2644delAA, 2645delAA, 2646delAC, 2647delCA, 2648delAA,
2649delAG, 2650delGC, 2651delCA, 2652delAT, 2653delTA, 2654delAG, 2655delGA,
2656delAA, 2657delAA, 2658delAT, 2659delTG, 2660delGG, 2661delGA, 2662delAA,
2663delAG, 2664delGA, 2665delAA, 2666delAA, 2667delAG, 2668delGT, 2669delTG,
2670delGA, 2671delAA, 2672delAC, 2673delCT, 2674delTT, 2675delTG, 2676delGA,
2677delAT, 2678delTG, 2679delGC, 2680delCT, 2681delTC, 2682delCA, 2683delAG,
2684delGT, 2685delTA, 2686delAT, 2687delTT, 2688delTT, 2689delTG, 2690delGC,
2691delCA, 2692delAG, 2693delGA, 2694delAA, 2695delAT, 2696delTA, 2697delAC,
2698delCA, 2699delAT, 2700delTT, 2701delTC, 2702delCA, 2703delAA, 2704delAG,
2705delGG, 2706delGT, 2707delTT, 2708delTT, 2709delTC, 2710delCA, 2711delAA,
2712delAA, 2713delAG, 2714delGC, 2715delCG, 2716delGC, 2717delCC, 2718delCA,
2719delAG, 2720delGT, 2721delTC, 2722delCA, 2723delAT, 2724delTT, 2725delTT,
2726delTG, 2727delGC, 2728delCT, 2729delTC, 2730delCT, 2731delTG, 2732delGT,
2733delTT, 2734delTT, 2735delTT, 2736delTC, 2737delCA, 2738delAA, 2739delAA,
2740delAT, 2741delTC, 2742delCC, 2743delCA, 2744delAG, 2745delGG, 2746delGA,
2747delAA, 2748delAA, 2749delAT, 2750delTG, 2751delGC, 2752delCA, 2753delAG,
2754delGA, 2755delAA, 2756delAG, 2757delGA, 2758delAG, 2759delGG, 2760delGA,
2761delAA, 2762delAT, 2763delTG, 2764delGT, 2765delTG, 2766delGC, 2767delCA,
2768delAA, 2769delAC, 2770delCA, 2771delAT, 2772delTT, 2773delTC, 2774delCT,
2775delTC, 2776delCT, 2777delTG, 2778delGC, 2779delCC, 2780delCC, 2781delCA,
2782delAC, 2783delCT, 2784delTC, 2785delCT, 2786delTG, 2787delGG, 2788delGG,
2789delGT, 2790delTC, 2791delCC, 2792delCT, 2793delTT, 2794delTA, 2795delAA,
2796delAA, 2797delAG, 2798delGA, 2799delAA, 2800delAA, 2801delAC, 2802delCA,
2803delAA, 2804delAA, 2805delAG, 2806delGT, 2807delTC, 2808delCC, 2809delCA,
2810delAA, 2811delAA, 2812delAA, 2813delAG, 2814delGT, 2815delTC, 2816delCA,
2817delAC, 2818delCT, 2819delTT, 2820delTT, 2821delTT, 2822delTG, 2823delGA,
2824delAA, 2825delAT, 2826delTG, 2827delGT, 2828delTG, 2829delGA, 2830delAA,
2831delAC, 2832delCA, 2833delAA, 2834delAA, 2835delAA, 2836delAG, 2837delGG,
2838delGA, 2839delAA, 2840delAG, 2841delGA, 2842delAA, 2843delAA, 2844delAA,
2845delAT, 2846delTC, 2847delCA, 2848delAA, 2849delAG, 2850delGG, 2851delGA,
2852delAA, 2853delAA, 2854delAG, 2855delGA, 2856delAA, 2857delAT, 2858delTG,
2859delGA, 2860delAG, 2861delGT, 2862delTC, 2863delCT, 2864delTA, 2865delAA,
2866delAT, 2867delTA, 2868delAT, 2869delTC, 2870delCA, 2871delAA, 2872delAG,
2873delGC, 2874delCC, 2875delCT, 2876delTG, 2877delGT, 2878delTA, 2879delAC,
2880delCA, 2881delAG, 2882delGA, 2883delAC, 2884delCA, 2885delAG, 2886delGT,
2887delTT, 2888delTA, 2889delAA, 2890delAT, 2891delTA, 2892delAT, 2893delTC,
2894delCA, 2895delAC, 2896delCT, 2897delTG, 2898delGC, 2899delCA, 2900delAG,
2901delGG, 2902delGC, 2903delCT, 2904delTT, 2905delTT, 2906delTC, 2907delCC,
2908delCT, 2909delTG, 2910delGT, 2911delTG, 2912delGG, 2913delGT, 2914delTT,
2915delTG, 2916delGG, 2917delGT, 2918delTC, 2919delCA, 2920delAG, 2921delGA,
2922delAA, 2923delAA, 2924delAG, 2925delGA, 2926delAT, 2927delTA, 2928delAA,
2929delAG, 2930delGC, 2931delCC, 2932delCA, 2933delAG, 2934delGT, 2935delTT,
2936delTG, 2937delGA, 2938delAT, 2939delTA, 2940delAA, 2941delAT, 2942delTG,
2943delGC, 2944delCC, 2945delCA, 2946delAA, 2947delAA, 2948delAT, 2949delTG,
2950delGT, 2951delTA, 2952delAG, 2953delGT, 2954delTA, 2955delAT, 2956delTC,
2957delCA, 2958delAA, 2959delAA, 2960delAG, 2961delGG, 2962delGA, 2963delAG,
2964delGG, 2965delGC, 2966delCT, 2967delTC, 2968delCT, 2969delTA, 2970delAG,
2971delGG, 2972delGT, 2973delTT, 2974delTT, 2975delTT, 2976delTG, 2977delGT,
2978delTC, 2979delCT, 2980delTA, 2981delAT, 2982delTC, 2983delCA, 2984delAT,
2985delTC, 2986delCT, 2987delTC, 2988delCA, 2989delAG, 2990delGT, 2991delTT,
2992delTC, 2993delCA, 2994delAG, 2995delGA, 2996delAG, 2997delGG, 2998delGC,
2999delCA, 3000delAA, 3001delAC, 3002delCG, 3003delGA, 3004delAA, 3005delAA,
3006delAC, 3007delCT, 3008delTG, 3009delGG, 3010delGA, 3011delAC, 3012delCT,
3013delTC, 3014delCA, 3015delAT, 3016delTT, 3017delTA, 3018delAC, 3019delCT,
3020delTC, 3021delCC, 3022delCA, 3023delAA, 3024delAA, 3025delAT, 3026delTA,
3027delAA, 3028delAA, 3029delAC, 3030delCA, 3031delAT, 3032delTG, 3033delGG,
3034delGA, 3035delAC, 3036delCT, 3037delTT, 3038delTT, 3039delTT, 3040delTA,
3041delAC, 3042delCA, 3043delAA, 3044delAA, 3045delAA, 3046delAC, 3047delCC,
3048delCC, 3049delCA, 3050delAT, 3051delTA, 3052delAT, 3053delTC, 3054delCG,
3055delGT, 3056delTA, 3057delAT, 3058delTA, 3059delAC, 3060delCC, 3061delCA,
3062delAC, 3063delCC, 3064delCA, 3065delAC, 3066delCT, 3067delTT, 3068delTT,
3069delTT, 3070delTT, 3071delTC, 3072delCC, 3073delCC, 3074delCA, 3075delAT,
3076delTC, 3077delCA, 3078delAA, 3079delAG, 3080delGT, 3081delTC, 3082delCA,
3083delAT, 3084delTT, 3085delTT, 3086delTG, 3087delGT, 3088delTT, 3089delTA,
3090delAA, 3091delAA, 3092delAA, 3093delAC, 3094delCT, 3095delTA, 3096delAA,
3097delAA, 3098delAT, 3099delTG, 3100delGT, 3101delTA, 3102delAA, 3103delAG,
3104delGA, 3105delAA, 3106delAA, 3107delAA, 3108delAA, 3109delAT, 3110delTC,
3111delCT, 3112delTG, 3113delGC, 3114delCT, 3115delTA, 3116delAG, 3117delGA,
3118delAG, 3119delGG, 3120delGA, 3121delAA, 3122delAA, 3123delAA, 3124delAC,
3125delCT, 3126delTT, 3127delTT, 3128delTG, 3129delGA, 3130delAG, 3131delGG,
3132delGA, 3133delAA, 3134delAC, 3135delCA, 3136delAT, 3137delTT, 3138delTC,
3139delCA, 3140delAA, 3141delAT, 3142delTG, 3143delGT, 3144delTC, 3145delCA,
3146delAC, 3147delCC, 3148delCT, 3149delTG, 3150delGA, 3151delAA, 3152delAA,
3153delAG, 3154delGA, 3155delAG, 3156delGA, 3157delAA, 3158delAA, 3159delAT,
3160delTG, 3161delGG, 3162delGG, 3163delGA, 3164delAA, 3165delAA, 3166delAT,
3167delTG, 3168delGA, 3169delAG, 3170delGA, 3171delAA, 3172delAC, 3173delCA,
3174delAT, 3175delTT, 3176delTC, 3177delCC, 3178delCA, 3179delAA, 3180delAG,
3181delGT, 3182delTA, 3183delAC, 3184delCA, 3185delAG, 3186delGT, 3187delTG,
3188delGA, 3189delAG, 3190delGC, 3191delCA, 3192delAC, 3193delCA, 3194delAA,
3195delAT, 3196delTT, 3197delTA, 3198delAG, 3199delGC, 3200delCC, 3201delCG,
3202delGT, 3203delTA, 3204delAA, 3205delAT, 3206delTA, 3207delAA, 3208delAC,
3209delCA, 3210delAT, 3211delTT, 3212delTA, 3213delAG, 3214delGA, 3215delAG,
3216delGA, 3217delAA, 3218delAA, 3219delAA, 3220delAT, 3221delTG, 3222delGT,
3223delTT, 3224delTT, 3225delTT, 3226delTT, 3227delTA, 3228delAA, 3229delAA,
3230delAG, 3231delGG, 3232delGA, 3233delAG, 3234delGC, 3235delCC, 3236delCA,
3237delAG, 3238delGC, 3239delCT, 3240delTC, 3241delCA, 3242delAA, 3243delAG,
3244delGC, 3245delCA, 3246delAA, 3247delAT, 3248delTA, 3249delAT, 3250delTT,
3251delTA, 3252delAA, 3253delAT, 3254delTG, 3255delGA, 3256delAA, 3257delAG,
3258delGT, 3259delTA, 3260delAG, 3261delGG, 3262delGT, 3263delTT, 3264delTC,
3265delCC, 3266delCA, 3267delAG, 3268delGT, 3269delTA, 3270delAC, 3271delCT,
3272delTA, 3273delAA, 3274delAT, 3275delTG, 3276delGA, 3277delAA, 3278delAG,
3279delGT, 3280delTG, 3281delGG, 3282delGG, 3283delGC, 3284delCT, 3285delTC,
3286delCC, 3287delCA, 3288delAG, 3289delGT, 3290delTA, 3291delAT, 3292delTT,
3293delTA, 3294delAA, 3295delAT, 3296delTG, 3297delGA, 3298delAA, 3299delAA,
3300delAT, 3301delTA, 3302delAG, 3303delGG, 3304delGT, 3305delTT, 3306delTC,
3307delCC, 3308delCA, 3309delAG, 3310delGT, 3311delTG, 3312delGA, 3313delAT,
3314delTG, 3315delGA, 3316delAA, 3317delAA, 3318delAA, 3319delAC, 3320delCA,
3321delAT, 3322delTT, 3323delTC, 3324delCA, 3325delAA, 3326delAG, 3327delGC,
3328delCA, 3329delAG, 3330delGA, 3331delAA, 3332delAC, 3333delCT, 3334delTA,
3335delAG, 3336delGG, 3337delGT, 3338delTA, 3339delAG, 3340delGA, 3341delAA,
3342delAA, 3343delAC, 3344delCA, 3345delAG, 3346delGA, 3347delAG, 3348delGG,
3349delGG, 3350delGC, 3351delCC, 3352delCA, 3353delAA, 3354delAA, 3355delAA,
3356delAT, 3357delTT, 3358delTG, 3359delGA, 3360delAA, 3361delAT, 3362delTG,
3363delGC, 3364delCT, 3365delTA, 3366delAT, 3367delTG, 3368delGC, 3369delCT,
3370delTT, 3371delTA, 3372delAG, 3373delGA, 3374delAT, 3375delTT, 3376delTA,
3377delAG, 3378delGG, 3379delGG, 3380delGG, 3381delGT, 3382delTT, 3383delTT,
3384delTT, 3385delTG, 3386delGC, 3387delCA, 3388delAA, 3389delAC, 3390delCC,
3391delCT, 3392delTG, 3393delGA, 3394delAG, 3395delGG, 3396delGT, 3397delTC,
3398delCT, 3399delTA, 3400delAT, 3401delTA, 3402delAA, 3403delAA, 3404delAC,
3405delCA, 3406delAA, 3407delAA, 3408delAG, 3409delGT, 3410delTC, 3411delCT,
3412delTT, 3413delTC, 3414delCC, 3415delCT, 3416delTG, 3417delGG, 3418delGA,
3419delAA, 3420delAG, 3421delGT, 3422delTA, 3423delAA, 3424delAT, 3425delTT,
3426delTG, 3427delGT, 3428delTA, 3429delAA, 3430delAG, 3431delGC, 3432delCA,
3433delAT, 3434delTC, 3435delCC, 3436delCT, 3437delTG, 3438delGA, 3439delAA,
3440delAA, 3441delAT, 3442delTA, 3443delAA, 3444delAA, 3445delAA, 3446delAA,
3447delAA, 3448delAG, 3449delGC, 3450delCA, 3451delAA, 3452delAG, 3453delGA,
3454delAA, 3455delAT, 3456delTA, 3457delAT, 3458delTG, 3459delGA, 3460delAA,
3461delAG, 3462delGA, 3463delAA, 3464delAG, 3465delGT, 3466delTA, 3467delAG,
3468delGT, 3469delTT, 3470delTC, 3471delCA, 3472delAG, 3473delGA, 3474delAC,
3475delCT, 3476delTG, 3477delGT, 3478delTT, 3479delTA, 3480delAA, 3481delAT,
3482delTA, 3483delAC, 3484delCA, 3485delAG, 3486delGA, 3487delAT, 3488delTT,
3489delTT, 3490delTC, 3491delCT, 3492delTC, 3493delCT, 3494delTC, 3495delCC,
3496delCA, 3497delAT, 3498delTA, 3499delAT, 3500delTC, 3501delCT, 3502delTG,
3503delGA, 3504delAT, 3505delTT, 3506delTT, 3507delTC, 3508delCA, 3509delAG,
3510delGA, 3511delAT, 3512delTA, 3513delAA, 3514delAC, 3515delCT, 3516delTT,
3517delTA, 3518delAG, 3519delGA, 3520delAA, 3521delAC, 3522delCA, 3523delAG,
3524delGC, 3525delCC, 3526delCT, 3527delTA, 3528delAT, 3529delTG, 3530delGG,
3531delGG, 3532delGA, 3533delAA, 3534delAG, 3535delGT, 3536delTA, 3537delAG,
3538delGT, 3539delTC, 3540delCA, 3541delAT, 3542delTG, 3543delGC, 3544delCA,
3545delAT, 3546delTC, 3547delCT, 3548delTC, 3549delCA, 3550delAG, 3551delGG,
3552delGT, 3553delTT, 3554delTT, 3555delTG, 3556delGT, 3557delTT, 3558delTC,
3559delCT, 3560delTG, 3561delGA, 3562delAG, 3563delGA, 3564delAC, 3565delCA,
3566delAC, 3567delCC, 3568delCT, 3569delTG, 3570delGA, 3571delAT, 3572delTG,
3573delGA, 3574delAC, 3575delCC, 3576delCT, 3577delTG, 3578delGT, 3579delTT,
3580delTA, 3581delAG, 3582delGA, 3583delAT, 3584delTG, 3585delGA, 3586delAT,
3587delTG, 3588delGG, 3589delGT, 3590delTG, 3591delGA, 3592delAA, 3593delAA,
3594delAT, 3595delTA, 3596delAA, 3597delAA, 3598delAG, 3599delGG, 3600delGA,
3601delAA, 3602delAG, 3603delGA, 3604delAT, 3605delTA, 3606delAC, 3607delCT,
3608delTA, 3609delAG, 3610delGT, 3611delTT, 3612delTT, 3613delTT, 3614delTG,
3615delGC, 3616delCT, 3617delTG, 3618delGA, 3619delAA, 3620delAA, 3621delAA,
3622delAT, 3623delTG, 3624delGA, 3625delAC, 3626delCA, 3627delAT, 3628delTT,
3629delTA, 3630delAA, 3631delAG, 3632delGG, 3633delGA, 3634delAA, 3635delAA,
3636delAG, 3637delGT, 3638delTT, 3639delTC, 3640delCT, 3641delTG, 3642delGC,
3643delCT, 3644delTG, 3645delGT, 3646delTT, 3647delTT, 3648delTT, 3649delTT,
3650delTA, 3651delAG, 3652delGC, 3653delCA, 3654delAA, 3655delAA, 3656delAA,
3657delAG, 3658delGC, 3659delCG, 3660delGT, 3661delTC, 3662delCC, 3663delCA,
3664delAG, 3665delGA, 3666delAG, 3667delGA, 3668delAG, 3669delGG, 3670delGA,
3671delAG, 3672delGA, 3673delAG, 3674delGC, 3675delCT, 3676delTT, 3677delTA,
3678delAG, 3679delGC, 3680delCA, 3681delAG, 3682delGG, 3683delGA, 3684delAG,
3685delGT, 3686delTC, 3687delCC, 3688delCT, 3689delTA, 3690delAG, 3691delGC,
3692delCC, 3693delCC, 3694delCT, 3695delTT, 3696delTT, 3697delTC, 3698delCA,
3699delAC, 3700delCC, 3701delCC, 3702delCA, 3703delAT, 3704delTA, 3705delAC,
3706delCA, 3707delAC, 3708delCA, 3709delAT, 3710delTT, 3711delTT, 3712delTG,
3713delGG, 3714delGC, 3715delCT, 3716delTC, 3717delCA, 3718delAG, 3719delGG,
3720delGG, 3721delGT, 3722delTT, 3723delTA, 3724delAC, 3725delCC, 3726delCG,
3727delGA, 3728delAA, 3729delAG, 3730delGA, 3731delAG, 3732delGG, 3733delGG,
3734delGG, 3735delGC, 3736delCC, 3737delCA, 3738delAA, 3739delAG, 3740delGA,
3741delAA, 3742delAA, 3743delAT, 3744delTT, 3745delTA, 3746delAG, 3747delGA,
3748delAG, 3749delGT, 3750delTC, 3751delCC, 3752delCT, 3753delTC, 3754delCA,
3755delAG, 3756delGA, 3757delAA, 3758delAG, 3759delGA, 3760delAG, 3761delGA,
3762delAA, 3763delAC, 3764delCT, 3765delTT, 3766delTA, 3767delAT, 3768delTC,
3769delCT, 3770delTA, 3771delAG, 3772delGT, 3773delTG, 3774delGA, 3775delAG,
3776delGG, 3777delGA, 3778delAT, 3779delTG, 3780delGA, 3781delAA, 3782delAG,
3783delGA, 3784delAG, 3785delGC, 3786delCT, 3787delTT, 3788delTC, 3789delCC,
3790delCC, 3791delCT, 3792delTG, 3793delGC, 3794delCT, 3795delTT, 3796delTC,
3797delCC, 3798delCA, 3799delAA, 3800delAC, 3801delCA, 3802delAC, 3803delCT,
3804delTT, 3805delTG, 3806delGT, 3807delTT, 3808delTA, 3809delAT, 3810delTT,
3811delTT, 3812delTG, 3813delGG, 3814delGT, 3815delTA, 3816delAA, 3817delAA,
3818delAG, 3819delGT, 3820delTA, 3821delAA, 3822delAA, 3823delAC, 3824delCA,
3825delAA, 3826delAT, 3827delTA, 3828delAT, 3829delTA, 3830delAC, 3831delCC,
3832delCT, 3833delTT, 3834delTC, 3835delCT, 3836delTC, 3837delCA, 3838delAG,
3839delGT, 3840delTC, 3841delCT, 3842delTA, 3843delAC, 3844delCT, 3845delTA,
3846delAG, 3847delGG, 3848delGC, 3849delCA, 3850delAT, 3851delTA, 3852delAG,
3853delGC, 3854delCA, 3855delAC, 3856delCC, 3857delCG, 3858delGT, 3859delTT,
3860delTG, 3861delGC, 3862delCT, 3863delTA, 3864delAC, 3865delCC, 3866delCG,
3867delGA, 3868delAG, 3869delGT, 3870delTG, 3871delGT, 3872delTC, 3873delCT,
3874delTG, 3875delGT, 3876delTC, 3877delCT, 3878delTA, 3879delAA, 3880delAG,
3881delGA, 3882delAA, 3883delAC, 3884delCA, 3885delAC, 3886delCA, 3887delAG,
3888delGA, 3889delAG, 3890delGG, 3891delGA, 3892delAG, 3893delGA, 3894delAA,
3895delAT, 3896delTT, 3897delTT, 3898delTA, 3899delAT, 3900delTT, 3901delTA,
3902delAT, 3903delTC, 3904delCA, 3905delAT, 3906delTT, 3907delTG, 3908delGA,
3909delAA, 3910delAG, 3911delGA, 3912delAA, 3913delAT, 3914delTA, 3915delAG,
3916delGC, 3917delCT, 3918delTT, 3919delTA, 3920delAA, 3921delAA, 3922delAT,
3923delTG, 3924delGA, 3925delAC, 3926delCT, 3927delTG, 3928delGC, 3929delCA,
3930delAG, 3931delGT, 3932delTA, 3933delAA, 3934delAC, 3935delCC, 3936delCA,
3937delAG, 3938delGG, 3939delGT, 3940delTA, 3941delAA, 3942delAT, 3943delTA,
3944delAT, 3945delTT, 3946delTG, 3947delGG, 3948delGC, 3949delCA, 3950delAA,
3951delAA, 3952delAG, 3953delGG, 3954delGC, 3955delCA, 3956delAT, 3957delTC,
3958delCT, 3959delTC, 3960delCA, 3961delAG, 3962delGG, 3963delGA, 3964delAA,
3965delAC, 3966delCA, 3967delAT, 3968delTC, 3969delCA, 3970delAC, 3971delCC,
3972delCT, 3973delTT, 3974delTA, 3975delAG, 3976delGT, 3977delTG, 3978delGA,
3979delAG, 3980delGG, 3981delGA, 3982delAA, 3983delAA, 3984delAC, 3985delCA,
3986delAA, 3987delAA, 3988delAA, 3989delAT, 3990delTG, 3991delGT, 3992delTT,
3993delTC, 3994delCT, 3995delTG, 3996delGC, 3997delCT, 3998delTA, 3999delAG,
4000delGC, 4001delCT, 4002delTT, 4003delTG, 4004delGT, 4005delTT, 4006delTT,
4007delTT, 4008delTC, 4009delCT, 4010delTT, 4011delTC, 4012delCA, 4013delAC,
4014delCA, 4015delAG, 4016delGT, 4017delTG, 4018delGC, 4019delCA, 4020delAG,
4021delGT, 4022delTG, 4023delGA, 4024delAA, 4025delAT, 4026delTT, 4027delTG,
4028delGG, 4029delGA, 4030delAA, 4031delAG, 4032delGA, 4033delAC, 4034delCT,
4035delTT, 4036delTG, 4037delGA, 4038delAC, 4039delCT, 4040delTG, 4041delGC,
4042delCA, 4043delAA, 4044delAA, 4045delAT, 4046delTA, 4047delAC, 4048delCA,
4049delAA, 4050delAA, 4051delAC, 4052delCA, 4053delAC, 4054delCC, 4055delCC,
4056delCA, 4057delAG, 4058delGG, 4059delGA, 4060delAT, 4061delTC, 4062delCC,
4063delCT, 4064delTT, 4065delTT, 4066delTC, 4067delCT, 4068delTT, 4069delTG,
4070delGA, 4071delAT, 4072delTT, 4073delTG, 4074delGG, 4075delGT, 4076delTT,
4077delTC, 4078delCT, 4079delTT, 4080delTC, 4081delCC, 4082delCA, 4083delAA,
4084delAA, 4085delAC, 4086delCA, 4087delAA, 4088delAA, 4089delAT, 4090delTG,
4091delGA, 4092delAG, 4093delGG, 4094delGC, 4095delCA, 4096delAT, 4097delTC,
4098delCA, 4099delAG, 4100delGT, 4101delTC, 4102delCT, 4103delTG, 4104delGA,
4105delAA, 4106delAA, 4107delAG, 4108delGC, 4109delCC, 4110delCA, 4111delAG,
4112delGG, 4113delGG, 4114delGA, 4115delAG, 4116delGT, 4117delTT, 4118delTG,
4119delGG, 4120delGT, 4121delTC, 4122delCT, 4123delTG, 4124delGA, 4125delAG,
4126delGT, 4127delTG, 4128delGA, 4129delAC, 4130delCA, 4131delAA, 4132delAG,
4133delGG, 4134delGA, 4135delAA, 4136delAT, 4137delTT, 4138delTG, 4139delGG,
4140delGT, 4141delTT, 4142delTT, 4143delTC, 4144delCA, 4145delAG, 4146delGA,
4147delAT, 4148delTG, 4149delGA, 4150delAT, 4151delTG, 4152delGA, 4153delAA,
4154delAG, 4155delGA, 4156delAA, 4157delAA, 4158delAG, 4159delGA, 4160delAG,
4161delGG, 4162delGA, 4163delAA, 4164delAC, 4165delCG, 4166delGG, 4167delGG,
4168delGC, 4169delCT, 4170delTT, 4171delTG, 4172delGG, 4173delGA, 4174delAA,
4175delAG, 4176delGA, 4177delAA, 4178delAA, 4179delAA, 4180delAT, 4181delTA,
4182delAA, 4183delAT, 4184delTC, 4185delCA, 4186delAA, 4187delAG, 4188delGA,
4189delAA, 4190delAG, 4191delGA, 4192delAG, 4193delGC, 4194delCA, 4195delAA,
4196delAA, 4197delAG, 4198delGC, 4199delCA, 4200delAT, 4201delTG, 4202delGG,
4203delGA, 4204delAT, 4205delTT, 4206delTC, 4207delCA, 4208delAA, 4209delAA,
4210delAC, 4211delCT, 4212delTT, 4213delTA, 4214delAG, 4215delGG, 4216delGT,
4217delTG, 4218delGA, 4219delAA, 4220delAG, 4221delGC, 4222delCA, 4223delAG,
4224delGC, 4225delCA, 4226delAT, 4227delTC, 4228delCT, 4229delTG, 4230delGG,
4231delGG, 4232delGT, 4233delTG, 4234delGT, 4235delTG, 4236delGA, 4237delAG,
4238delGA, 4239delAG, 4240delGT, 4241delTG, 4242delGA, 4243delAA, 4244delAA,
4245delAC, 4246delCA, 4247delAA, 4248delAG, 4249delGC, 4250delCG, 4251delGT,
4252delTC, 4253delCT, 4254delTC, 4255delCT, 4256delTG, 4257delGA, 4258delAA,
4259delAG, 4260delGA, 4261delAC, 4262delCT, 4263delTG, 4264delGC, 4265delCT,
4266delTC, 4267delCA, 4268delAG, 4269delGG, 4270delGG, 4271delGC, 4272delCT,
4273delTA, 4274delAT, 4275delTC, 4276delCC, 4277delCT, 4278delTC, 4279delCT,
4280delTC, 4281delCA, 4282delAG, 4283delGA, 4284delAG, 4285delGT, 4286delTG,
4287delGA, 4288delAC, 4289delCA, 4290delAT, 4291delTT, 4292delTT, 4293delTT,
4294delTA, 4295delAA, 4296delAC, 4297delCC, 4298delCA, 4299delAC, 4300delCT,
4301delTC, 4302delCA, 4303delAG, 4304delGC, 4305delCA, 4306delAG, 4307delGA,
4308delAG, 4309delGG, 4310delGG, 4311delGA, 4312delAT, 4313delTA, 4314delAC,
4315delCC, 4316delCA, 4317delAT, 4318delTG, 4319delGC, 4320delCA, 4321delAA,
4322delAC, 4323delCA, 4324delAT, 4325delTA, 4326delAA, 4327delAC, 4328delCC,
4329delCT, 4330delTG, 4331delGA, 4332delAT, 4333delTA, 4334delAA, 4335delAA,
4336delAG, 4337delGC, 4338delCT, 4339delTC, 4340delCC, 4341delCA, 4342delAG,
4343delGC, 4344delCA, 4345delAG, 4346delGG, 4347delGA, 4348delAA, 4349delAA,
4350delAT, 4351delTG, 4352delGG, 4353delGC, 4354delCT, 4355delTG, 4356delGA,
4357delAA, 4358delAC, 4359delCT, 4360delTA, 4361delAG, 4362delGA, 4363delAA,
4364delAG, 4365delGC, 4366delCT, 4367delTG, 4368delGT, 4369delTG, 4370delGT,
4371delTT, 4372delTA, 4373delAG, 4374delGA, 4375delAA, 4376delAC, 4377delCA,
4378delAG, 4379delGC, 4380delCA, 4381delAT, 4382delTG, 4383delGG, 4384delGG,
4385delGA, 4386delAG, 4387delGC, 4388delCC, 4389delCA, 4390delAG, 4391delGC,
4392delCC, 4393delCT, 4394delTT, 4395delTC, 4396delCT, 4397delTA, 4398delAA,
4399delAC, 4400delCA, 4401delAG, 4402delGC, 4403delCT, 4404delTA, 4405delAC,
4406delCC, 4407delCC, 4408delCT, 4409delTT, 4410delTC, 4411delCC, 4412delCA,
4413delAT, 4414delTC, 4415delCA, 4416delAT, 4417delTA, 4418delAA, 4419delAG,
4420delGT, 4421delTG, 4422delGA, 4423delAC, 4424delCT, 4425delTC, 4426delCC,
4427delCT, 4428delTC, 4429delCT, 4430delTG, 4431delGC, 4432delCC, 4433delCC,
4434delCT, 4435delTT, 4436delTG, 4437delGA, 4438delAG, 4439delGG, 4440delGA,
4441delAC, 4442delCC, 4443delCT, 4444delTG, 4445delGC, 4446delCG, 4447delGA,
4448delAA, 4449delAA, 4450delAT, 4451delTC, 4452delCC, 4453delCA, 4454delAG,
4455delGA, 4456delAA, 4457delAC, 4458delCA, 4459delAA, 4460delAA, 4461delAG,
4462delGC, 4463delCA, 4464delAC, 4465delCA, 4466delAT, 4467delTC, 4468delCA,
4469delAG, 4470delGA, 4471delAA, 4472delAA, 4473delAA, 4474delAA, 4475delAG,
4476delGC, 4477delCA, 4478delAG, 4479delGT, 4480delTA, 4481delAT, 4482delTT,
4483delTA, 4484delAA, 4485delAC, 4486delCT, 4487delTT, 4488delTC, 4489delCA,
4490delAC, 4491delCA, 4492delAG, 4493delGA, 4494delAA, 4495delAA, 4496delAA,
4497delAG, 4498delGT, 4499delTA, 4500delAG, 4501delGT, 4502delTG, 4503delGA,
4504delAA, 4505delAT, 4506delTA, 4507delAC, 4508delCC, 4509delCC, 4510delCT,
4511delTA, 4512delAT, 4513delTA, 4514delAA, 4515delAG, 4516delGC, 4517delCC,
4518delCA, 4519delAG, 4520delGA, 4521delAA, 4522delAT, 4523delTC, 4524delCC,
4525delCA, 4526delAG, 4527delGA, 4528delAA, 4529delAG, 4530delGG, 4531delGC,
4532delCC, 4533delCT, 4534delTT, 4535delTT, 4536delTC, 4537delCT, 4538delTG,
4539delGC, 4540delCT, 4541delTG, 4542delGA, 4543delAC, 4544delCA, 4545delAA,
4546delAG, 4547delGT, 4548delTT, 4549delTT, 4550delTG, 4551delGA, 4552delAG,
4553delGG, 4554delGT, 4555delTG, 4556delGT, 4557delTC, 4558delCT, 4559delTG,
4560delGC, 4561delCA, 4562delAG, 4563delGA, 4564delAT, 4565delTA, 4566delAG,
4567delGT, 4568delTT, 4569delTC, 4570delCT, 4571delTA, 4572delAC, 4573delCC,
4574delCA, 4575delAG, 4576delGT, 4577delTA, 4578delAA, 4579delAA, 4580delAA,
4581delAA, 4582delAT, 4583delTA, 4584delAA, 4585delAA, 4586delAG, 4587delGA,
4588delAA, 4589delAC, 4590delCC, 4591delCA, 4592delAG, 4593delGG, 4594delGA,
4595delAG, 4596delGT, 4597delTG, 4598delGG, 4599delGA, 4600delAA, 4601delAA,
4602delAG, 4603delGG, 4604delGT, 4605delTC, 4606delCA, 4607delAT, 4608delTC,
4609delCC, 4610delCC, 4611delCC, 4612delCT, 4613delTT, 4614delTC, 4615delCT,
4616delTA, 4617delAA, 4618delAA, 4619delAT, 4620delTG, 4621delGC, 4622delCC,
4623delCC, 4624delCA, 4625delAT, 4626delTC, 4627delCA, 4628delAT, 4629delTT,
4630delTA, 4631delAG, 4632delGA, 4633delAT, 4634delTG, 4635delGA, 4636delAT,
4637delTA, 4638delAG, 4639delGG, 4640delGT, 4641delTG, 4642delGG, 4643delGT,
4644delTA, 4645delAC, 4646delCA, 4647delAT, 4648delTG, 4649delGC, 4650delCA,
4651delAC, 4652delCA, 4653delAG, 4654delGT, 4655delTT, 4656delTG, 4657delGC,
4658delCT, 4659delTC, 4660delCT, 4661delTG, 4662delGG, 4663delGG, 4664delGA,
4665delAG, 4666delGT, 4667delTC, 4668delCT, 4669delTT, 4670delTC, 4671delCA,
4672delAG, 4673delGA, 4674delAA, 4675delAT, 4676delTA, 4677delAG, 4678delGA,
4679delAA, 4680delAA, 4681delAC, 4682delCT, 4683delTA, 4684delAC, 4685delCC,
4686delCC, 4687delCA, 4688delAT, 4689delTC, 4690delCT, 4691delTC, 4692delCA,
4693delAA, 4694delAG, 4695delGA, 4696delAG, 4697delGG, 4698delGA, 4699delAG,
4700delGC, 4701delCT, 4702delTC, 4703delCA, 4704delAT, 4705delTT, 4706delTA,
4707delAA, 4708delAG, 4709delGG, 4710delGT, 4711delTT, 4712delTG, 4713delGT,
4714delTT, 4715delTG, 4716delGA, 4717delAT, 4718delTG, 4719delGT, 4720delTG,
4721delGG, 4722delGA, 4723delAG, 4724delGG, 4725delGA, 4726delAG, 4727delGC,
4728delCA, 4729delAA, 4730delAC, 4731delCA, 4732delAG, 4733delGC, 4734delCT,
4735delTG, 4736delGG, 4737delGA, 4738delAA, 4739delAG, 4740delGA, 4741delAG,
4742delGT, 4743delTC, 4744delCT, 4745delTG, 4746delGG, 4747delGG, 4748delGC,
4749delCC, 4750delCA, 4751delAC, 4752delCA, 4753delAC, 4754delCG, 4755delGA,
4756delAT, 4757delTT, 4758delTT, 4759delTG, 4760delGA, 4761delAC, 4762delCG,
4763delGG, 4764delGA, 4765delAA, 4766delAA, 4767delAC, 4768delCA, 4769delAT,
4770delTC, 4771delCT, 4772delTT, 4773delTA, 4774delAC, 4775delCT, 4776delTT,
4777delTG, 4778delGC, 4779delCC, 4280delCA, 4781delAA, 4782delAG, 4783delGG,
4784delGC, 4785delCA, 4786delAA, 4787delAG, 4788delGA, 4789delAT, 4790delTC,
4791delCT, 4792delTA, 4793delAG, 4794delGA, 4795delAG, 4796delGG, 4797delGG,
4798delGA, 4799delAA, 4800delAC, 4801delCC, 4802delCC, 4803delCC, 4804delCT,
4805delTT, 4806delTA, 4807delAC, 4808delCC, 4809delCT, 4810delTG, 4811delGG,
4812delGA, 4813delAA, 4814delAT, 4815delTC, 4816delCT, 4817delTG, 4818delGG,
4819delGA, 4820delAA, 4821delAT, 4822delTC, 4823delCA, 4824delAG, 4825delGC,
4826delCC, 4827delCT, 4828delTC, 4829delCT, 4830delTT, 4831delTC, 4832delCT,
4833delTC, 4834delCT, 4835delTG, 4836delGA, 4837delAT, 4838delTG, 4839delGA,
4840delAC, 4841delCC, 4842delCC, 4843delCT, 4844delTG, 4845delGA, 4846delAA,
4847delAT, 4848delTC, 4849delCT, 4850delTG, 4851delGA, 4852delAT, 4853delTC,
4854delCC, 4855delCT, 4856delTT, 4857delTC, 4858delCT, 4859delTG, 4860delGA,
4861delAA, 4862delAG, 4863delGA, 4864delAC, 4865delCA, 4866delAG, 4867delGA,
4868delAG, 4869delGC, 4870delCC, 4871delCC, 4872delCC, 4873delCA, 4874delAG,
4875delGA, 4876delAG, 4877delGT, 4878delTC, 4879delCA, 4880delAG, 4881delGC,
4882delCT, 4883delTC, 4884delCG, 4885delGT, 4886delTG, 4887delGT, 4888delTT,
4889delTG, 4890delGG, 4891delGC, 4892delCA, 4893delAA, 4894delAC, 4895delCA,
4896delAT, 4897delTA, 4898delAC, 4899delCC, 4900delCA, 4901delAT, 4902delTC,
4903delCT, 4904delTT, 4905delTC, 4906delCA, 4907delAA, 4908delAC, 4909delCC,
4910delCT, 4911delTC, 4912delCT, 4913delTG, 4914delGC, 4915delCA, 4916delAT,
4917delTT, 4918delTG, 4919delGA, 4920delAA, 4921delAA, 4922delAG, 4923delGT,
4924delTT, 4925delTC, 4926delCC, 4927delCC, 4928delCC, 4929delCA, 4930delAA,
4931delAT, 4932delTT, 4933delTG, 4934delGA, 4935delAA, 4936delAA, 4937delAG,
4938delGT, 4939delTT, 4940delTG, 4941delGC, 4942delCA, 4943delAG, 4944delGA,
4945delAA, 4946delAT, 4947delTC, 4948delCT, 4949delTG, 4950delGC, 4951delCC,
4952delCC, 4953delCA, 4954delAG, 4955delGG, 4956delGG, 4957delGT, 4958delTC,
4959delCC, 4960delCA, 4961delAG, 4962delGC, 4963delCT, 4964delTG, 4965delGC,
4966delCT, 4967delTG, 4968delGC, 4969delCT, 4970delTC, 4971delCA, 4972delAT,
4973delTA, 4974delAC, 4975delCT, 4976delTA, 4977delAC, 4978delCT, 4979delTG,
4980delGA, 4981delAT, 4982delTA, 4983delAC, 4984delCT, 4985delTG, 4986delGC,
4987delCT, 4988delTG, 4989delGG, 4990delGG, 4991delGT, 4992delTA, 4993delAT,
4994delTA, 4995delAA, 4996delAT, 4997delTG, 4998delGC, 4999delCA, 5000delAA,
5001delAT, 5002delTG, 5003delGG, 5004delGA, 5005delAA, 5006delAG, 5007delGA,
5008delAA, 5009delAA, 5010delAG, 5011delGT, 5012delTG, 5013delGT, 5014delTG,
5015delGA, 5016delAG, 5017delGC, 5018delCA, 5019delAG, 5020delGG, 5021delGG,
5022delGA, 5023delAG, 5024delGA, 5025delAA, 5026delAG, 5027delGC, 5028delCC,
5029delCA, 5030delAG, 5031delGA, 5032delAA, 5033delAT, 5034delTT, 5035delTG,
5036delGA, 5037delAC, 5038delCA, 5039delAG, 5040delGC, 5041delCT, 5042delTT,
5043delTC, 5044delCA, 5045delAA, 5046delAC, 5047delCA, 5048delAG, 5049delGA,
5050delAA, 5051delAA, 5052delAG, 5053delGG, 5054delGG, 5055delGT, 5056delTC,
5057delCA, 5058delAA, 5059delAC, 5060delCA, 5061delAA, 5062delAA, 5063delAA,
5064delAG, 5065delGA, 5066delAA, 5067delAT, 5068delTG, 5069delGT, 5070delTC,
5071delCC, 5072delCA, 5073delAT, 5074delTG, 5075delGG, 5076delGT, 5077delTG,
5078delGG, 5079delGT, 5080delTG, 5081delGT, 5082delTC, 5083delCT, 5084delTG,
5085delGG, 5086delGC, 5087delCC, 5088delCT, 5089delTG, 5090delGA, 5091delAC,
5092delCC, 5093delCC, 5094delCC, 5095delCA, 5096delAG, 5097delGA, 5098delAA,
5099delAG, 5100delGA, 5101delAA, 5102delAT, 5103delTT, 5104delTT, 5105delTA,
5106delAT, 5107delTG, 5108delGC, 5109delCT, 5110delTC, 5111delCG, 5112delGT,
5113delTG, 5114delGT, 5115delTA, 5116delAC, 5117delCA, 5118delAA, 5119delAG,
5120delGT, 5121delTT, 5122delTT, 5123delTG, 5124delGC, 5125delCC, 5126delCA,
5127delAG, 5128delGA, 5129delAA, 5130delAA, 5131delAA, 5132delAC, 5133delCA,
5134delAC, 5135delCC, 5136delCA, 5137delAC, 5138delCA, 5139delAT, 5140delTC,
5141delCA, 5142delAC, 5143delCT, 5144delTT, 5145delTT, 5146delTA, 5147delAA,
5148delAC, 5149delCT, 5150delTA, 5151delAA, 5152delAT, 5153delTC, 5154delCT,
5155delTA, 5156delAA, 5157delAT, 5158delTT, 5159delTA, 5160delAC, 5161delCT,
5162delTG, 5163delGA, 5164delAA, 5165delAG, 5166delGA, 5167delAG, 5168delGA,
5169delAC, 5170delCT, 5171delTA, 5172delAC, 5173delCT, 5174delTC, 5175delCA,
5176delAT, 5177delTG, 5178delGT, 5179delTT, 5180delTG, 5181delGT, 5182delTT,
5183delTA, 5184delAT, 5185delTG, 5186delGA, 5187delAA, 5188delAA, 5189delAA,
5190delAC, 5191delCA, 5192delAG, 5193delGA, 5194delAT, 5195delTG, 5196delGC,
5197delCT, 5198delTG, 5199delGA, 5200delAG, 5201delGT, 5202delTT, 5203delTT,
5204delTG, 5205delGT, 5206delTG, 5207delGT, 5208delTG, 5209delGT, 5210delTG,
5211delGA, 5212delAA, 5213delAC, 5214delCG, 5215delGG, 5216delGA, 5217delAC,
5218delCA, 5219delAC, 5220delCT, 5221delTG, 5222delGA, 5223delAA, 5224delAA,
5225delAT, 5226delTA, 5227delAT, 5228delTT, 5229delTT, 5230delTT, 5231delTC,
5232delCT, 5233delTA, 5234delAG, 5235delGG, 5236delGA, 5237delAA, 5238delAT,
5239delTT, 5240delTG, 5241delGC, 5242delCG, 5243delGG, 5244delGG, 5245delGA,
5246delAG, 5247delGG, 5248delGA, 5249delAA, 5250delAA, 5251delAA, 5252delAT,
5253delTG, 5254delGG, 5255delGG, 5256delGT, 5257delTA, 5258delAG, 5259delGT,
5260delTT, 5261delTA, 5262delAG, 5263delGC, 5264delCT, 5265delTA, 5266delAT,
5267delTT, 5268delTT, 5269delTC, 5270delCT, 5271delTG, 5272delGG, 5273delGG,
5274delGT, 5275delTG, 5276delGA, 5277delAC, 5278delCC, 5279delCC, 5280delCA,
5281delAG, 5282delGT, 5283delTC, 5284delCT, 5285delTA, 5286delAT, 5287delTT,
5288delTA, 5289delAA, 5290delAA, 5291delAG, 5292delGA, 5293delAA, 5294delAA,
5295delAG, 5296delGA, 5297delAA, 5298delAA, 5299delAA, 5300delAA, 5301delAT,
5302delTG, 5303delGC, 5304delCT, 5305delTG, 5306delGA, 5307delAA, 5308delAT,
5309delTG, 5310delGA, 5311delAG, 5312delGC, 5313delCA, 5314delAT, 5315delTG,
5316delGA, 5317delAT, 5318delTT, 5319delTT, 5320delTT, 5321delTG, 5322delGA,
5323delAA, 5324delAG, 5325delGT, 5126delTC, 5327delCA, 5328delAG, 5329delGA,
5330delAG, 5331delGG, 5332delGA, 5333delAG, 5334delGA, 5335delAT, 5336delTG,
5337delGT, 5338delTG, 5339delGG, 5340delGT, 5341delTC, 5342delCA, 5343delAA,
5344delAT, 5345delTG, 5346delGG, 5347delGA, 5348delAA, 5349delAG, 5350delGA,
5351delAA, 5352delAA, 5353delAC, 5354delCC, 5355delCA, 5356delAC, 5357delCC,
5358delCA, 5359delAA, 5360delAG, 5361delGG, 5362delGT, 5363delTC, 5364delCC,
5365delCA, 5366delAA, 5367delAA, 5368delAG, 5369delGC, 5370delCG, 5371delGA,
5372delAG, 5373delGC, 5374delCA, 5375delAA, 5376delAG, 5377delGA, 5378delAG,
5379delGA, 5380delAA, 5381delAT, 5382delTC, 5383delCC, 5384delCC, 5385delCA,
5386delAG, 5387delGG, 5388delGA, 5389delAC, 5390delCA, 5391delAG, 5392delGA,
5393delAA, 5394delAA, 5395delAG, 5396delGA, 5397delAT, 5398delTC, 5399delCT,
5400delTT, 5401delTC, 5402delCA, 5403delAG, 5404delGG, 5405delGG, 5406delGG,
5407delGG, 5408delGC, 5409delCT, 5410delTA, 5411delAG, 5412delGA, 5413delAA,
5414delAA, 5415delAT, 5416delTC, 5417delCT, 5418delTG, 5419delGT, 5420delTT,
5421delTG, 5422delGC, 5423delCT, 5424delTA, 5425delAT, 5426delTG, 5427delGG,
5428delGG, 5429delGC, 5430delCC, 5431delCC, 5432delCT, 5433delTT, 5434delTC,
5435delCA, 5436delAC, 5437delCC, 5438delCA, 5439delAA, 5440delAC, 5441delCA,
5442delAT, 5443delTG, 5444delGC, 5445delCC, 5446delCC, 5447delCA, 5448delAC,
5449delCA, 5450delAG, 5451delGA, 5452delAT, 5453delTC, 5454delCA, 5455delAA,
5456delAC, 5457delCT, 5458delTG, 5459delGG, 5460delGA, 5461delAA, 5462delAT,
5463delTG, 5464delGG, 5465delGA, 5466delAT, 5467delTG, 5468delGG, 5469delGT,
5470delTA, 5471delAC, 5472delCA, 5473delAG, 5474delGC, 5475delCT, 5476delTG,
5477delGT, 5478delTG, 5479delGT, 5480delTG, 5481delGG, 5482delGT, 5483delTG,
5484delGC, 5485delCT, 5486delTT, 5487delTC, 5488delCT, 5489delTG, 5490delGT,
5491delTG, 5492delGG, 5493delGT, 5494delTG, 5495delGA, 5496delAA, 5497delAG,
5498delGG, 5499delGA, 5500delAG, 5501delGC, 5502delCT, 5503delTT, 5504delTT,
5505delTC, 5506delCA, 5507delAT, 5508delTC, 5509delCA, 5510delAT, 5511delTT,
5512delTC, 5513delCA, 5514delAC, 5515delCC, 5516delCC, 5517delCT, 5518delTT,
5519delTG, 5520delGG, 5521delGC, 5522delCA, 5523delAC, 5524delCA, 5525delAG,
5526delGG, 5527delGT, 5528delTG, 5529delGT, 5530delTC, 5531delCC, 5532delCA,
5533delAC, 5534delCC, 5535delCC, 5536delCA, 5537delAA, 5538delAT, 5539delTT,
5540delTG, 5541delGT, 5542delTG, 5543delGG, 5544delGT, 5545delTT, 5546delTG,
5547delGT, 5548delTG, 5549delGC, 5550delCA, 5551delAG, 5552delGC, 5553delCC,
5554delCA, 5555delAG, 5556delGA, 5557delAT, 5558delTG, 5559delGC, 5560delCC,
5561delCT, 5562delTG, 5563delGG, 5564delGA, 5565delAC, 5566delCA, 5567delAG,
5568delGA, 5569delAG, 5570delGG, 5571delGA, 5572delAC, 5573delCA, 5574delAA,
5575delAT, 5576delTG, 5577delGG, 5578delGC, 5579delCT, 5580delTT, 5581delTC,
5582delCC, 5583delCA, 5584delAT, 5585delTG, 5586delGC, 5587delCA, 5588delAA,
5589delAT, 5590delTT, 5591delTG, 5592delGG, 5593delGG, 5594delGC, 5595delCA,
5596delAG, 5597delGA, 5598delAT, 5599delTG, 5600delGT, 5601delTG, 5602delGT,
5603delTG, 5635delTG, 5654delCC, 5660delCC, 5708delCT.

[0229]

TABLE 11
List of One Base Insertions
(N = A, T, G, or C)
122insN, 123insN, 124insN, 125insN, 126insN, 127insN, 128insN, 129insN, 130insN, 131insN,
132insN, 133insN, 134insN, 135insN, 136insN, 137insN, 138insN, 139insN, 140insN, 141insN,
142insN, 143insN, 144insN, 145insN, 146insN, 147insN, 148insN, 149insN, 150insN, 151insN,
152insN, 153insN, 154insN, 155insN, 156insN, 157insN, 158insN, 159insN, 160insN, 161insN,
162insN, 163insN, 164insN, 165insN, 166insN, 167insN, 168insN, 169insN, 170insN, 171insN,
172insN, 173insN, 174insN, 175insN, 176insN, 177insN, 178insN, 179insN, 180insN, 181insN,
182insN, 183insN, 184insN, 185insN, 186insN, 187insN, 188insN, 189insN, 190insN, 191insN,
192insN, 193insN, 194insN, 195insN, 196insN, 197insN, 198insN, 199insN, 200insN, 201insN,
202insN, 203insN, 204insN, 205insN, 206insN, 207insN, 208insN, 209insN, 210insN, 211insN,
212insN, 213insN, 214insN, 215insN, 216insN, 217insN, 218insN, 219insN, 220insN, 221insN,
222insN, 223insN, 224insN, 225insN, 226insN, 227insN, 228insN, 229insN, 230insN, 231insN,
232insN, 233insN, 234insN, 235insN, 236insN, 237insN, 238insN, 239insN, 240insN, 241insN,
242insN, 243insN, 244insN, 245insN, 246insN, 247insN, 248insN, 249insN, 250insN, 251insN,
252insN, 253insN, 254insN, 255insN, 256insN, 257insN, 258insN, 259insN, 260insN, 261insN,
262insN, 263insN, 264insN, 265insN, 266insN, 267insN, 268insN, 269insN, 270insN, 271insN,
272insN, 273insN, 274insN, 275insN, 276insN, 277insN, 278insN, 279insN, 280insN, 281insN,
282insN, 283insN, 284insN, 285insN, 286insN, 287insN, 288insN, 289insN, 290insN, 291insN,
292insN, 293insN, 294insN, 295insN, 296insN, 297insN, 298insN, 299insN, 300insN, 301insN,
302insN, 303insN, 304insN, 305insN, 306insN, 307insN, 308insN, 309insN, 310insN, 311insN,
312insN, 313insN, 314insN, 315insN, 316insN, 317insN, 318insN, 319insN, 320insN, 321insN,
322insN, 323insN, 324insN, 325insN, 326insN, 327insN, 328insN, 329insN, 330insN, 331insN,
332insN, 333insN, 334insN, 335insN, 336insN, 337insN, 338insN, 339insN, 340insN, 341insN,
342insN, 343insN, 344insN, 345insN, 346insN, 347insN, 348insN, 349insN, 350insN, 351insN,
352insN, 353insN, 354insN, 355insN, 356insN, 357insN, 358insN, 359insN, 360insN, 361insN,
362insN, 363insN, 364insN, 365insN, 366insN, 367insN, 368insN, 369insN, 370insN, 371insN,
372insN, 373insN, 374insN, 375insN, 376insN, 377insN, 378insN, 379insN, 380insN, 381insN,
382insN, 383insN, 384insN, 385insN, 386insN, 387insN, 388insN, 389insN, 390insN, 391insN,
392insN, 393insN, 394insN, 395insN, 396insN, 397insN, 398insN, 399insN, 400insN, 401insN,
402insN, 403insN, 404insN, 405insN, 406insN, 407insN, 408insN, 409insN, 410insN, 411insN,
412insN, 413insN, 414insN, 415insN, 416insN, 417insN, 418insN, 419insN, 420insN, 421insN,
422insN, 423insN, 424insN, 425insN, 426insN, 427insN, 428insN, 429insN, 430insN, 431insN,
432insN, 433insN, 434insN, 435insN, 436insN, 437insN, 438insN, 439insN, 440insN, 441insN,
442insN, 443insN, 444insN, 445insN, 446insN, 447insN, 448insN, 449insN, 450insN, 451insN,
452insN, 453insN, 454insN, 455insN, 456insN, 457insN, 458insN, 459insN, 460insN, 461insN,
462insN, 463insN, 464insN, 465insN, 466insN, 467insN, 468insN, 469insN, 470insN, 471insN,
472insN, 473insN, 474insN, 475insN, 476insN, 477insN, 478insN, 479insN, 480insN, 481insN,
482insN, 483insN, 484insN, 485insN, 486insN, 487insN, 488insN, 489insN, 490insN, 491insN,
492insN, 493insN, 494insN, 495insN, 496insN, 497insN, 498insN, 499insN, 500insN, 501insN,
502insN, 503insN, 504insN, 505insN, 506insN, 507insN, 508insN, 509insN, 510insN, 511insN,
512insN, 513insN, 514insN, 515insN, 516insN, 517insN, 518insN, 519insN, 520insN, 521insN,
522insN, 523insN, 524insN, 525insN, 526insN, 527insN, 528insN, 529insN, 530insN, 531insN,
532insN, 533insN, 534insN, 535insN, 536insN, 537insN, 538insN, 539insN, 540insN, 541insN,
542insN, 543insN, 544insN, 545insN, 546insN, 547insN, 548insN, 549insN, 550insN, 551insN,
552insN, 553insN, 554insN, 555insN, 556insN, 557insN, 558insN, 559insN, 560insN, 561insN,
562insN, 563insN, 564insN, 565insN, 566insN, 567insN, 568insN, 569insN, 570insN, 571insN,
572insN, 573insN, 574insN, 575insN, 576insN, 577insN, 578insN, 579insN, 580insN, 581insN,
582insN, 583insN, 584insN, 585insN, 586insN, 587insN, 588insN, 589insN, 590insN, 591insN,
592insN, 593insN, 594insN, 595insN, 596insN, 597insN, 598insN, 599insN, 600insN, 601insN,
602insN, 603insN, 604insN, 605insN, 606insN, 607insN, 608insN, 609insN, 610insN, 611insN,
612insN, 613insN, 614insN, 615insN, 616insN, 617insN, 618insN, 619insN, 620insN, 621insN,
622insN, 623insN, 624insN, 625insN, 626insN, 627insN, 628insN, 629insN, 630insN, 631insN,
632insN, 633insN, 634insN, 635insN, 636insN, 637insN, 638insN, 639insN, 640insN, 641insN,
642insN, 643insN, 644insN, 645insN, 646insN, 647insN, 648insN, 649insN, 650insN, 651insN,
652insN, 653insN, 654insN, 655insN, 656insN, 657insN, 658insN, 659insN, 660insN, 661insN,
662insN, 663insN, 664insN, 665insN, 666insN, 667insN, 668insN, 669insN, 670insN, 671insN,
672insN, 673insN, 674insN, 675insN, 676insN, 677insN, 678insN, 679insN, 680insN, 681insN,
682insN, 683insN, 684insN, 685insN, 686insN, 687insN, 688insN, 689insN, 690insN, 691insN,
692insN, 693insN, 694insN, 695insN, 696insN, 697insN, 698insN, 699insN, 700insN, 701insN,
702insN, 703insN, 704insN, 705insN, 706insN, 707insN, 708insN, 709insN, 710insN, 711insN,
712insN, 713insN, 714insN, 715insN, 716insN, 717insN, 718insN, 719insN, 720insN, 721insN,
722insN, 723insN, 724insN, 725insN, 726insN, 727insN, 728insN, 729insN, 730insN, 731insN,
732insN, 733insN, 734insN, 735insN, 736insN, 737insN, 738insN, 739insN, 740insN, 741insN,
742insN, 743insN, 744insN, 745insN, 746insN, 747insN, 748insN, 749insN, 750insN, 751insN,
752insN, 753insN, 754insN, 755insN, 756insN, 757insN, 758insN, 759insN, 760insN, 761insN,
762insN, 763insN, 764insN, 765insN, 766insN, 767insN, 768insN, 769insN, 770insN, 771insN,
772insN, 773insN, 774insN, 775insN, 776insN, 777insN, 778insN, 779insN, 780insN, 781insN,
782insN, 783insN, 784insN, 785insN, 786insN, 787insN, 788insN, 789insN, 790insN, 791insN,
792insN, 793insN, 794insN, 795insN, 796insN, 797insN, 798insN, 799insN, 800insN, 801insN,
802insN, 803insN, 804insN, 805insN, 806insN, 807insN, 808insN, 809insN, 810insN, 811insN,
812insN, 813insN, 814insN, 815insN, 816insN, 817insN, 818insN, 819insN, 820insN, 821insN,
822insN, 823insN, 824insN, 825insN, 826insN, 827insN, 828insN, 829insN, 830insN, 831insN,
832insN, 833insN, 834insN, 835insN, 836insN, 837insN, 838insN, 839insN, 840insN, 841insN,
842insN, 843insN, 844insN, 845insN, 846insN, 847insN, 848insN, 849insN, 850insN, 851insN,
852insN, 853insN, 854insN, 855insN, 856insN, 857insN, 858insN, 859insN, 860insN, 861insN,
862insN, 863insN, 864insN, 865insN, 866insN, 867insN, 868insN, 869insN, 870insN, 871insN,
872insN, 873insN, 874insN, 875insN, 876insN, 877insN, 878insN, 879insN, 880insN, 881insN,
882insN, 883insN, 884insN, 885insN, 886insN, 887insN, 888insN, 889insN, 890insN, 891insN,
892insN, 893insN, 894insN, 895insN, 896insN, 897insN, 898insN, 899insN, 900insN, 901insN,
902insN, 903insN, 904insN, 905insN, 906insN, 907insN, 908insN, 909insN, 910insN, 911insN,
912insN, 913insN, 914insN, 915insN, 916insN, 917insN, 918insN, 919insN, 920insN, 921insN,
922insN, 923insN, 924insN, 925insN, 926insN, 927insN, 928insN, 929insN, 930insN, 931insN,
932insN, 933insN, 934insN, 935insN, 936insN, 937insN, 938insN, 939insN, 940insN, 941insN,
942insN, 943insN, 944insN, 945insN, 946insN, 947insN, 948insN, 949insN, 950insN, 951insN,
952insN, 953insN, 954insN, 955insN, 956insN, 957insN, 958insN, 959insN, 960insN, 961insN,
962insN, 963insN, 964insN, 965insN, 966insN, 967insN, 968insN, 969insN, 970insN, 971insN,
972insN, 973insN, 974insN, 975insN, 976insN, 977insN, 978insN, 979insN, 980insN, 981insN,
982insN, 983insN, 984insN, 985insN, 986insN, 987insN, 988insN, 989insN, 990insN, 991insN,
992insN, 993insN, 994insN, 995insN, 996insN, 997insN, 998insN, 999insN, 1000insN,
1001insN, 1002insN, 1003insN, 1004insN, 1005insN, 1006insN, 1007insN, 1008insN,
1009insN, 1010insN, 1011insN, 1012insN, 1013insN, 1014insN, 1015insN, 1016insN,
1017insN, 1018insN, 1019insN, 1020insN, 1021insN, 1022insN, 1023insN, 1024insN,
1025insN, 1026insN, 1027insN, 1028insN, 1029insN, 1030insN, 1031insN, 1032insN,
1033insN, 1034insN, 1035insN, 1036insN, 1037insN, 1038insN, 1039insN, 1040insN,
1041insN, 1042insN, 1043insN, 1044insN, 1045insN, 1046insN, 1047insN, 1048insN,
1049insN, 1050insN, 1051insN, 1052insN, 1053insN, 1054insN, 1055insN, 1056insN,
1057insN, 1058insN, 1059insN, 1060insN, 1061insN, 1062insN, 1063insN, 1064insN,
1065insN, 1066insN, 1067insN, 1068insN, 1069insN, 1070insN, 1071insN, 1072insN,
1073insN, 1074insN, 1075insN, 1076insN, 1077insN, 1078insN, 1079insN, 1080insN,
1081insN, 1082insN, 1083insN, 1084insN, 1085insN, 1086insN, 1087insN, 1088insN,
1089insN, 1090insN, 1091insN, 1092insN, 1093insN, 1094insN, 1095insN, 1096insN,
1097insN, 1098insN, 1099insN, 1100insN, 1101insN, 1102insN, 1103insN, 1104insN,
1105insN, 1106insN, 1107insN, 1108insN, 1109insN, 1110insN, 1111insN, 1112insN,
1113insN, 1114insN, 1115insN, 1116insN, 1117insN, 1118insN, 1119insN, 1120insN,
1121insN, 1122insN, 1123insN, 1124insN, 1125insN, 1126insN, 1127insN, 1128insN,
1129insN, 1130insN, 1131insN, 1132insN, 1133insN, 1134insN, 1135insN, 1136insN,
1137insN, 1138insN, 1139insN, 1140insN, 1141insN, 1142insN, 1143insN, 1144insN,
1145insN, 1146insN, 1147insN, 1148insN, 1149insN, 1150insN, 1151insN, 1152insN,
1153insN, 1154insN, 1155insN, 1156insN, 1157insN, 1158insN, 1159insN, 1160insN,
1161insN, 1162insN, 1163insN, 1164insN, 1165insN, 1166insN, 1167insN, 1168insN,
1169insN, 1170insN, 1171insN, 1172insN, 1173insN, 1174insN, 1175insN, 1176insN,
1177insN, 1178insN, 1179insN, 1180insN, 1181insN, 1182insN, 1183insN, 1184insN,
1185insN, 1186insN, 1187insN, 1188insN, 1189insN, 1190insN, 1191insN, 1192insN,
1193insN, 1194insN, 1195insN, 1196insN, 1197insN, 1198insN, 1199insN, 1200insN,
1201insN, 1202insN, 1203insN, 1204insN, 1205insN, 1206insN, 1207insN, 1208insN,
1209insN, 1210insN, 1211insN, 1212insN, 1213insN, 1214insN, 1215insN, 1216insN,
1217insN, 1218insN, 1219insN, 1220insN, 1221insN, 1222insN, 1223insN, 1224insN,
1225insN, 1226insN, 1227insN, 1228insN, 1229insN, 1230insN, 1231insN, 1232insN,
1233insN, 1234insN, 1235insN, 1236insN, 1237insN, 1238insN, 1239insN, 1240insN,
1241insN, 1242insN, 1243insN, 1244insN, 1245insN, 1246insN, 1247insN, 1248insN,
1249insN, 1250insN, 1251insN, 1252insN, 1253insN, 1254insN, 1255insN, 1256insN,
1257insN, 1258insN, 1259insN, 1260insN, 1261insN, 1262insN, 1263insN, 1264insN,
1265insN, 1266insN, 1267insN, 1268insN, 1269insN, 1270insN, 1271insN, 1272insN,
1273insN, 1274insN, 1275insN, 1276insN, 1277insN, 1278insN, 1279insN, 1280insN,
1281insN, 1282insN, 1283insN, 1284insN, 1285insN, 1286insN, 1287insN, 1288insN,
1289insN, 1290insN, 1291insN, 1292insN, 1293insN, 1294insN, 1295insN, 1296insN,
1297insN, 1298insN, 1299insN, 1300insN, 1301insN, 1302insN, 1303insN, 1304insN,
1305insN, 1306insN, 1307insN, 1308insN, 1309insN, 1310insN, 1311insN, 1312insN,
1313insN, 1314insN, 1315insN, 1316insN, 1317insN, 1318insN, 1319insN, 1320insN,
1321insN, 1322insN, 1323insN, 1324insN, 1325insN, 1326insN, 1327insN, 1328insN,
1329insN, 1330insN, 1331insN, 1332insN, 1333insN, 1334insN, 1335insN, 1336insN,
1337insN, 1338insN, 1339insN, 1340insN, 1341insN, 1342insN, 1343insN, 1344insN,
1345insN, 1346insN, 1347insN, 1348insN, 1349insN, 1350insN, 1351insN, 1352insN,
1353insN, 1354insN, 1355insN, 1356insN, 1357insN, 1358insN, 1359insN, 1360insN,
1361insN, 1362insN, 1363insN, 1364insN, 1365insN, 1366insN, 1367insN, 1368insN,
1369insN, 1370insN, 1371insN, 1372insN, 1373insN, 1374insN, 1375insN, 1376insN,
1377insN, 1378insN, 1379insN, 1380insN, 1381insN, 1382insN, 1383insN, 1384insN,
1385insN, 1386insN, 1387insN, 1388insN, 1389insN, 1390insN, 1391insN, 1392insN,
1393insN, 1394insN, 1395insN, 1396insN, 1397insN, 1398insN, 1399insN, 1400insN,
1401insN, 1402insN, 1403insN, 1404insN, 1405insN, 1406insN, 1407insN, 1408insN,
1409insN, 1410insN, 1411insN, 1412insN, 1413insN, 1414insN, 1415insN, 1416insN,
1417insN, 1418insN, 1419insN, 1420insN, 1421insN, 1422insN, 1423insN, 1424insN,
1425insN, 1426insN, 1427insN, 1428insN, 1429insN, 1430insN, 1431insN, 1432insN,
1433insN, 1434insN, 1435insN, 1436insN, 1437insN, 1438insN, 1439insN, 1440insN,
1441insN, 1442insN, 1443insN, 1444insN, 1445insN, 1446insN, 1447insN, 1448insN,
1449insN, 1450insN, 1451insN, 1452insN, 1453insN, 1454insN, 1455insN, 1456insN,
1457insN, 1458insN, 1459insN, 1460insN, 1461insN, 1462insN, 1463insN, 1464insN,
1465insN, 1466insN, 1467insN, 1468insN, 1469insN, 1470insN, 1471insN, 1472insN,
1473insN, 1474insN, 1475insN, 1476insN, 1477insN, 1478insN, 1479insN, 1480insN,
1481insN, 1482insN, 1483insN, 1484insN, 1485insN, 1486insN, 1487insN, 1488insN,
1489insN, 1490insN, 1491insN, 1492insN, 1493insN, 1494insN, 1495insN, 1496insN,
1497insN, 1498insN, 1499insN, 1500insN, 1501insN, 1502insN, 1503insN, 1504insN,
1505insN, 1506insN, 1507insN, 1508insN, 1509insN, 1510insN, 1511insN, 1512insN,
1513insN, 1514insN, 1515insN, 1516insN, 1517insN, 1518insN, 1519insN, 1520insN,
1521insN, 1522insN, 1523insN, 1524insN, 1525insN, 1526insN, 1527insN, 1528insN,
1529insN, 1530insN, 1531insN, 1532insN, 1533insN, 1534insN, 1535insN, 1536insN,
1537insN, 1538insN, 1539insN, 1540insN, 1541insN, 1542insN, 1543insN, 1544insN,
1545insN, 1546insN, 1547insN, 1548insN, 1549insN, 1550insN, 1551insN, 1552insN,
1553insN, 1554insN, 1555insN, 1556insN, 1557insN, 1558insN, 1559insN, 1560insN,
1561insN, 1562insN, 1563insN, 1564insN, 1565insN, 1566insN, 1567insN, 1568insN,
1569insN, 1570insN, 1571insN, 1572insN, 1573insN, 1574insN, 1575insN, 1576insN,
1577insN, 1578insN, 1579insN, 1580insN, 1581insN, 1582insN, 1583insN, 1584insN,
1585insN, 1586insN, 1587insN, 1588insN, 1589insN, 1590insN, 1591insN, 1592insN,
1593insN, 1594insN, 1595insN, 1596insN, 1597insN, 1598insN, 1599insN, 1600insN,
1601insN, 1602insN, 1603insN, 1604insN, 1605insN, 1606insN, 1607insN, 1608insN,
1609insN, 1610insN, 1611insN, 1612insN, 1613insN, 1614insN, 1615insN, 1616insN,
1617insN, 1618insN, 1619insN, 1620insN, 1621insN, 1622insN, 1623insN, 1624insN,
1625insN, 1626insN, 1627insN, 1628insN, 1629insN, 1630insN, 1631insN, 1632insN,
1633insN, 1634insN, 1635insN, 1636insN, 1637insN, 1638insN, 1639insN, 1640insN,
1641insN, 1642insN, 1643insN, 1644insN, 1645insN, 1646insN, 1647insN, 1648insN,
1649insN, 1650insN, 1651insN, 1652insN, 1653insN, 1654insN, 1655insN, 1656insN,
1657insN, 1658insN, 1659insN, 1660insN, 1661insN, 1662insN, 1663insN, 1664insN,
1665insN, 1666insN, 1667insN, 1668insN, 1669insN, 1670insN, 1671insN, 1672insN,
1673insN, 1674insN, 1675insN, 1676insN, 1677insN, 1678insN, 1679insN, 1680insN,
1681insN, 1682insN, 1683insN, 1684insN, 1685insN, 1686insN, 1687insN, 1688insN,
1689insN, 1690insN, 1691insN, 1692insN, 1693insN, 1694insN, 1695insN, 1696insN,
1697insN, 1698insN, 1699insN, 1700insN, 1701insN, 1702insN, 1703insN, 1704insN,
1705insN, 1706insN, 1707insN, 1708insN, 1709insN, 1710insN, 1711insN, 1712insN,
1713insN, 1714insN, 1715insN, 1716insN, 1717insN, 1718insN, 1719insN, 1720insN,
1721insN, 1722insN, 1723insN, 1724insN, 1725insN, 1726insN, 1727insN, 1728insN,
1729insN, 1730insN, 1731insN, 1732insN, 1733insN, 1734insN, 1735insN, 1736insN,
1737insN, 1738insN, 1739insN, 1740insN, 1741insN, 1742insN, 1743insN, 1744insN,
1745insN, 1746insN, 1747insN, 1748insN, 1749insN, 1750insN, 1751insN, 1752insN,
1753insN, 1754insN, 1755insN, 1756insN, 1757insN, 1758insN, 1759insN, 1760insN,
1761insN, 1762insN, 1763insN, 1764insN, 1765insN, 1766insN, 1767insN, 1768insN,
1769insN, 1770insN, 1771insN, 1772insN, 1773insN, 1774insN, 1775insN, 1776insN,
1777insN, 1778insN, 1779insN, 1780insN, 1781insN, 1782insN, 1783insN, 1784insN,
1785insN, 1786insN, 1787insN, 1788insN, 1789insN, 1790insN, 1791insN, 1792insN,
1793insN, 1794insN, 1795insN, 1796insN, 1797insN, 1798insN, 1799insN, 1800insN,
1801insN, 1802insN, 1803insN, 1804insN, 1805insN, 1806insN, 1807insN, 1808insN,
1809insN, 1810insN, 1811insN, 1812insN, 1813insN, 1814insN, 1815insN, 1816insN,
1817insN, 1818insN, 1819insN, 1820insN, 1821insN, 1822insN, 1823insN, 1824insN,
1825insN, 1826insN, 1827insN, 1828insN, 1829insN, 1830insN, 1831insN, 1832insN,
1833insN, 1834insN, 1835insN, 1836insN, 1837insN, 1838insN, 1839insN, 1840insN,
1841insN, 1842insN, 1843insN, 1844insN, 1845insN, 1846insN, 1847insN, 1848insN,
1849insN, 1850insN, 1851insN, 1852insN, 1853insN, 1854insN, 1855insN, 1856insN,
1857insN, 1858insN, 1859insN, 1860insN, 1861insN, 1862insN, 1863insN, 1864insN,
1865insN, 1866insN, 1867insN, 1868insN, 1869insN, 1870insN, 1871insN, 1872insN,
1873insN, 1874insN, 1875insN, 1876insN, 1877insN, 1878insN, 1879insN, 1880insN,
1881insN, 1882insN, 1883insN, 1884insN, 1885insN, 1886insN, 1887insN, 1888insN,
1889insN, 1890insN, 1891insN, 1892insN, 1893insN, 1894insN, 1895insN, 1896insN,
1897insN, 1898insN, 1899insN, 1900insN, 1901insN, 1902insN, 1903insN, 1904insN,
1905insN, 1906insN, 1907insN, 1908insN, 1909insN, 1910insN, 1911insN, 1912insN,
1913insN, 1914insN, 1915insN, 1916insN, 1917insN, 1918insN, 1919insN, 1920insN,
1921insN, 1922insN, 1923insN, 1924insN, 1925insN, 1926insN, 1927insN, 1928insN,
1929insN, 1930insN, 1931insN, 1932insN, 1933insN, 1934insN, 1935insN, 1936insN,
1937insN, 1938insN, 1939insN, 1940insN, 1941insN, 1942insN, 1943insN, 1944insN,
1945insN, 1946insN, 1947insN, 1948insN, 1949insN, 1950insN, 1951insN, 1952insN,
1953insN, 1954insN, 1955insN, 1956insN, 1957insN, 1958insN, 1959insN, 1960insN,
1961insN, 1962insN, 1963insN, 1964insN, 1965insN, 1966insN, 1967insN, 1968insN,
1969insN, 1970insN, 1971insN, 1972insN, 1973insN, 1974insN, 1975insN, 1976insN,
1977insN, 1978insN, 1979insN, 1980insN, 1981insN, 1982insN, 1983insN, 1984insN,
1985insN, 1986insN, 1987insN, 1988insN, 1989insN, 1990insN, 1991insN, 1992insN,
1993insN, 1994insN, 1995insN, 1996insN, 1997insN, 1998insN, 1999insN, 2000insN,
2001insN, 2002insN, 2003insN, 2004insN, 2005insN, 2006insN, 2007insN, 2008insN,
2009insN, 2010insN, 2011insN, 2012insN, 2013insN, 2014insN, 2015insN, 2016insN,
2017insN, 2018insN, 2019insN, 2020insN, 2021insN, 2022insN, 2023insN, 2024insN,
2025insN, 2026insN, 2027insN, 2028insN, 2029insN, 2030insN, 2031insN, 2032insN,
2033insN, 2034insN, 2035insN, 2036insN, 2037insN, 2038insN, 2039insN, 2040insN,
2041insN, 2042insN, 2043insN, 2044insN, 2045insN, 2046insN, 2047insN, 2048insN,
2049insN, 2050insN, 2051insN, 2052insN, 2053insN, 2054insN, 2055insN, 2056insN,
2057insN, 2058insN, 2059insN, 2060insN, 2061insN, 2062insN, 2063insN, 2064insN,
2065insN, 2066insN, 2067insN, 2068insN, 2069insN, 2070insN, 2071insN, 2072insN,
2073insN, 2074insN, 2075insN, 2076insN, 2077insN, 2078insN, 2079insN, 2080insN,
2081insN, 2082insN, 2083insN, 2084insN, 2085insN, 2086insN, 2087insN, 2088insN,
2089insN, 2090insN, 2091insN, 2092insN, 2093insN, 2094insN, 2095insN, 2096insN,
2097insN, 2098insN, 2099insN, 2100insN, 2101insN, 2102insN, 2103insN, 2104insN,
2105insN, 2106insN, 2107insN, 2108insN, 2109insN, 2110insN, 2111insN, 2112insN,
2113insN, 2114insN, 2115insN, 2116insN, 2117insN, 2118insN, 2119insN, 2120insN,
2121insN, 2122insN, 2123insN, 2124insN, 2125insN, 2126insN, 2127insN, 2128insN,
2129insN, 2130insN, 2131insN, 2132insN, 2133insN, 2134insN, 2135insN, 2136insN,
2137insN, 2138insN, 2139insN, 2140insN, 2141insN, 2142insN, 2143insN, 2144insN,
2145insN, 2146insN, 2147insN, 2148insN, 2149insN, 2150insN, 2151insN, 2152insN,
2153insN, 2154insN, 2155insN, 2156insN, 2157insN, 2158insN, 2159insN, 2160insN,
2161insN, 2162insN, 2163insN, 2164insN, 2165insN, 2166insN, 2167insN, 2168insN,
2169insN, 2170insN, 2171insN, 2172insN, 2173insN, 2174insN, 2175insN, 2176insN,
2177insN, 2178insN, 2179insN, 2180insN, 2181insN, 2182insN, 2183insN, 2184insN,
2185insN, 2186insN, 2187insN, 2188insN, 2189insN, 2190insN, 2191insN, 2192insN,
2193insN, 2194insN, 2195insN, 2196insN, 2197insN, 2198insN, 2199insN, 2200insN,
2201insN, 2202insN, 2203insN, 2204insN, 2205insN, 2206insN, 2207insN, 2208insN,
2209insN, 2210insN, 2211insN, 2212insN, 2213insN, 2214insN, 2215insN, 2216insN,
2217insN, 2218insN, 2219insN, 2220insN, 2221insN, 2222insN, 2223insN, 2224insN,
2225insN, 2226insN, 2227insN, 2228insN, 2229insN, 2230insN, 2231insN, 2232insN,
2233insN, 2234insN, 2235insN, 2236insN, 2237insN, 2238insN, 2239insN, 2240insN,
2241insN, 2242insN, 2243insN, 2244insN, 2245insN, 2246insN, 2247insN, 2248insN,
2249insN, 2250insN, 2251insN, 2252insN, 2253insN, 2254insN, 2255insN, 2256insN,
2257insN, 2258insN, 2259insN, 2260insN, 2261insN, 2262insN, 2263insN, 2264insN,
2265insN, 2266insN, 2267insN, 2268insN, 2269insN, 2270insN, 2271insN, 2272insN,
2273insN, 2274insN, 2275insN, 2276insN, 2277insN, 2278insN, 2279insN, 2280insN,
2281insN, 2282insN, 2283insN, 2284insN, 2285insN, 2286insN, 2287insN, 2288insN,
2289insN, 2290insN, 2291insN, 2292insN, 2293insN, 2294insN, 2295insN, 2296insN,
2297insN, 2298insN, 2299insN, 2300insN, 2301insN, 2302insN, 2303insN, 2304insN,
2305insN, 2306insN, 2307insN, 2308insN, 2309insN, 2310insN, 2311insN, 2312insN,
2313insN, 2314insN, 2315insN, 2316insN, 2317insN, 2318insN, 2319insN, 2320insN,
2321insN, 2322insN, 2323insN, 2324insN, 2325insN, 2326insN, 2327insN, 2328insN,
2329insN, 2330insN, 2331insN, 2332insN, 2333insN, 2334insN, 2335insN, 2336insN,
2337insN, 2338insN, 2339insN, 2340insN, 2341insN, 2342insN, 2343insN, 2344insN,
2345insN, 2346insN, 2347insN, 2348insN, 2349insN, 2350insN, 2351insN, 2352insN,
2353insN, 2354insN, 2355insN, 2356insN, 2357insN, 2358insN, 2359insN, 2360insN,
2361insN, 2362insN, 2363insN, 2364insN, 2365insN, 2366insN, 2367insN, 2368insN,
2369insN, 2370insN, 2371insN, 2372insN, 2373insN, 2374insN, 2375insN, 2376insN,
2377insN, 2378insN, 2379insN, 2380insN, 2381insN, 2382insN, 2383insN, 2384insN,
2385insN, 2386insN, 2387insN, 2388insN, 2389insN, 2390insN, 2391insN, 2392insN,
2393insN, 2394insN, 2395insN, 2396insN, 2397insN, 2398insN, 2399insN, 2400insN,
2401insN, 2402insN, 2403insN, 2404insN, 2405insN, 2406insN, 2407insN, 2408insN,
2409insN, 2410insN, 2411insN, 2412insN, 2413insN, 2414insN, 2415insN, 2416insN,
2417insN, 2418insN, 2419insN, 2420insN, 2421insN, 2422insN, 2423insN, 2424insN,
2425insN, 2426insN, 2427insN, 2428insN, 2429insN, 2430insN, 2431insN, 2432insN,
2433insN, 2434insN, 2435insN, 2436insN, 2437insN, 2438insN, 2439insN, 2440insN,
2441insN, 2442insN, 2443insN, 2444insN, 2445insN, 2446insN, 2447insN, 2448insN,
2449insN, 2450insN, 2451insN, 2452insN, 2453insN, 2454insN, 2455insN, 2456insN,
2457insN, 2458insN, 2459insN, 2460insN, 2461insN, 2462insN, 2463insN, 2464insN,
2465insN, 2466insN, 2467insN, 2468insN, 2469insN, 2470insN, 2471insN, 2472insN,
2473insN, 2474insN, 2475insN, 2476insN, 2477insN, 2478insN, 2479insN, 2480insN,
2481insN, 2482insN, 2483insN, 2484insN, 2485insN, 2486insN, 2487insN, 2488insN,
2489insN, 2490insN, 2491insN, 2492insN, 2493insN, 2494insN, 2495insN, 2496insN,
2497insN, 2498insN, 2499insN, 2500insN, 2501insN, 2502insN, 2503insN, 2504insN,
2505insN, 2506insN, 2507insN, 2508insN, 2509insN, 2510insN, 2511insN, 2512insN,
2513insN, 2514insN, 2515insN, 2516insN, 2517insN, 2518insN, 2519insN, 2520insN,
2521insN, 2522insN, 2523insN, 2524insN, 2525insN, 2526insN, 2527insN, 2528insN,
2529insN, 2530insN, 2531insN, 2532insN, 2533insN, 2534insN, 2535insN, 2536insN,
2537insN, 2538insN, 2539insN, 2540insN, 2541insN, 2542insN, 2543insN, 2544insN,
2545insN, 2546insN, 2547insN, 2548insN, 2549insN, 2550insN, 2551insN, 2552insN,
2553insN, 2554insN, 2555insN, 2556insN, 2557insN, 2558insN, 2559insN, 2560insN,
2561insN, 2562insN, 2563insN, 2564insN, 2565insN, 2566insN, 2567insN, 2568insN,
2569insN, 2570insN, 2571insN, 2572insN, 2573insN, 2574insN, 2575insN, 2576insN,
2577insN, 2578insN, 2579insN, 2580insN, 2581insN, 2582insN, 2583insN, 2584insN,
2585insN, 2586insN, 2587insN, 2588insN, 2589insN, 2590insN, 2591insN, 2592insN,
2593insN, 2594insN, 2595insN, 2596insN, 2597insN, 2598insN, 2599insN, 2600insN,
2601insN, 2602insN, 2603insN, 2604insN, 2605insN, 2606insN, 2607insN, 2608insN,
2609insN, 2610insN, 2611insN, 2612insN, 2613insN, 2614insN, 2615insN, 2616insN,
2617insN, 2618insN, 2619insN, 2620insN, 2621insN, 2622insN, 2623insN, 2624insN,
2625insN, 2626insN, 2627insN, 2628insN, 2629insN, 2630insN, 2631insN, 2632insN,
2633insN, 2634insN, 2635insN, 2636insN, 2637insN, 2638insN, 2639insN, 2640insN,
2641insN, 2642insN, 2643insN, 2644insN, 2645insN, 2646insN, 2647insN, 2648insN,
2649insN, 2650insN, 2651insN, 2652insN, 2653insN, 2654insN, 2655insN, 2656insN,
2657insN, 2658insN, 2659insN, 2660insN, 2661insN, 2662insN, 2663insN, 2664insN,
2665insN, 2666insN, 2667insN, 2668insN, 2669insN, 2670insN, 2671insN, 2672insN,
2673insN, 2674insN, 2675insN, 2676insN, 2677insN, 2678insN, 2679insN, 2680insN,
2681insN, 2682insN, 2683insN, 2684insN, 2685insN, 2686insN, 2687insN, 2688insN,
2689insN, 2690insN, 2691insN, 2692insN, 2693insN, 2694insN, 2695insN, 2696insN,
2697insN, 2698insN, 2699insN, 2700insN, 2701insN, 2702insN, 2703insN, 2704insN,
2705insN, 2706insN, 2707insN, 2708insN, 2709insN, 2710insN, 2711insN, 2712insN,
2713insN, 2714insN, 2715insN, 2716insN, 2717insN, 2718insN, 2719insN, 2720insN,
2721insN, 2722insN, 2723insN, 2724insN, 2725insN, 2726insN, 2727insN, 2728insN,
2729insN, 2730insN, 2731insN, 2732insN, 2733insN, 2734insN, 2735insN, 2736insN,
2737insN, 2738insN, 2739insN, 2740insN, 2741insN, 2742insN, 2743insN, 2744insN,
2745insN, 2746insN, 2747insN, 2748insN, 2749insN, 2750insN, 2751insN, 2752insN,
2753insN, 2754insN, 2755insN, 2756insN, 2757insN, 2758insN, 2759insN, 2760insN,
2761insN, 2762insN, 2763insN, 2764insN, 2765insN, 2766insN, 2767insN, 2768insN,
2769insN, 2770insN, 2771insN, 2772insN, 2773insN, 2774insN, 2775insN, 2776insN,
2777insN, 2778insN, 2779insN, 2780insN, 2781insN, 2782insN, 2783insN, 2784insN,
2785insN, 2786insN, 2787insN, 2788insN, 2789insN, 2790insN, 2791insN, 2792insN,
2793insN, 2794insN, 2795insN, 2796insN, 2797insN, 2798insN, 2799insN, 2800insN,
2801insN, 2802insN, 2803insN, 2804insN, 2805insN, 2806insN, 2807insN, 2808insN,
2809insN, 2810insN, 2811insN, 2812insN, 2813insN, 2814insN, 2815insN, 2816insN,
2817insN, 2818insN, 2819insN, 2820insN, 2821insN, 2822insN, 2823insN, 2824insN,
2825insN, 2826insN, 2827insN, 2828insN, 2829insN, 2830insN, 2831insN, 2832insN,
2833insN, 2834insN, 2835insN, 2836insN, 2837insN, 2838insN, 2839insN, 2840insN,
2841insN, 2842insN, 2843insN, 2844insN, 2845insN, 2846insN, 2847insN, 2848insN,
2849insN, 2850insN, 2851insN, 2852insN, 2853insN, 2854insN, 2855insN, 2856insN,
2857insN, 2858insN, 2859insN, 2860insN, 2861insN, 2862insN, 2863insN, 2864insN,
2865insN, 2866insN, 2867insN, 2868insN, 2869insN, 2870insN, 2871insN, 2872insN,
2873insN, 2874insN, 2875insN, 2876insN, 2877insN, 2878insN, 2879insN, 2880insN,
2881insN, 2882insN, 2883insN, 2884insN, 2885insN, 2886insN, 2887insN, 2888insN,
2889insN, 2890insN, 2891insN, 2892insN, 2893insN, 2894insN, 2895insN, 2896insN,
2897insN, 2898insN, 2899insN, 2900insN, 2901insN, 2902insN, 2903insN, 2904insN,
2905insN, 2906insN, 2907insN, 2908insN, 2909insN, 2910insN, 2911insN, 2912insN,
2913insN, 2914insN, 2915insN, 2916insN, 2917insN, 2918insN, 2919insN, 2920insN,
2921insN, 2922insN, 2923insN, 2924insN, 2925insN, 2926insN, 2927insN, 2928insN,
2929insN, 2930insN, 2931insN, 2932insN, 2933insN, 2934insN, 2935insN, 2936insN,
2937insN, 2938insN, 2939insN, 2940insN, 2941insN, 2942insN, 2943insN, 2944insN,
2945insN, 2946insN, 2947insN, 2948insN, 2949insN, 2950insN, 2951insN, 2952insN,
2953insN, 2954insN, 2955insN, 2956insN, 2957insN, 2958insN, 2959insN, 2960insN,
2961insN, 2962insN, 2963insN, 2964insN, 2965insN, 2966insN, 2967insN, 2968insN,
2969insN, 2970insN, 2971insN, 2972insN, 2973insN, 2974insN, 2975insN, 2976insN,
2977insN, 2978insN, 2979insN, 2980insN, 2981insN, 2982insN, 2983insN, 2984insN,
2985insN, 2986insN, 2987insN, 2988insN, 2989insN, 2990insN, 2991insN, 2992insN,
2993insN, 2994insN, 2995insN, 2996insN, 2997insN, 2998insN, 2999insN, 3000insN,
3001insN, 3002insN, 3003insN, 3004insN, 3005insN, 3006insN, 3007insN, 3008insN,
3009insN, 3010insN, 3011insN, 3012insN, 3013insN, 3014insN, 3015insN, 3016insN,
3017insN, 3018insN, 3019insN, 3020insN, 3021insN, 3022insN, 3023insN, 3024insN,
3025insN, 3026insN, 3027insN, 3028insN, 3029insN, 3030insN, 3031insN, 3032insN,
3033insN, 3034insN, 3035insN, 3036insN, 3037insN, 3038insN, 3039insN, 3040insN,
3041insN, 3042insN, 3043insN, 3044insN, 3045insN, 3046insN, 3047insN, 3048insN,
3049insN, 3050insN, 3051insN, 3052insN, 3053insN, 3054insN, 3055insN, 3056insN,
3057insN, 3058insN, 3059insN, 3060insN, 3061insN, 3062insN, 3063insN, 3064insN,
3065insN, 3066insN, 3067insN, 3068insN, 3069insN, 3070insN, 3071insN, 3072insN,
3073insN, 3074insN, 3075insN, 3076insN, 3077insN, 3078insN, 3079insN, 3080insN,
3081insN, 3082insN, 3083insN, 3084insN, 3085insN, 3086insN, 3087insN, 3088insN,
3089insN, 3090insN, 3091insN, 3092insN, 3093insN, 3094insN, 3095insN, 3096insN,
3097insN, 3098insN, 3099insN, 3100insN, 3101insN, 3102insN, 3103insN, 3104insN,
3105insN, 3106insN, 3107insN, 3108insN, 3109insN, 3110insN, 3111insN, 3112insN,
3113insN, 3114insN, 3115insN, 3116insN, 3117insN, 3118insN, 3119insN, 3120insN,
3121insN, 3122insN, 3123insN, 3124insN, 3125insN, 3126insN, 3127insN, 3128insN,
3129insN, 3130insN, 3131insN, 3132insN, 3133insN, 3134insN, 3135insN, 3136insN,
3137insN, 3138insN, 3139insN, 3140insN, 3141insN, 3142insN, 3143insN, 3144insN,
3145insN, 3146insN, 3147insN, 3148insN, 3149insN, 3150insN, 3151insN, 3152insN,
3153insN, 3154insN, 3155insN, 3156insN, 3157insN, 3158insN, 3159insN, 3160insN,
3161insN, 3162insN, 3163insN, 3164insN, 3165insN, 3166insN, 3167insN, 3168insN,
3169insN, 3170insN, 3171insN, 3172insN, 3173insN, 3174insN, 3175insN, 3176insN,
3177insN, 3178insN, 3179insN, 3180insN, 3181insN, 3182insN, 3183insN, 3184insN,
3185insN, 3186insN, 3187insN, 3188insN, 3189insN, 3190insN, 3191insN, 3192insN,
3193insN, 3194insN, 3195insN, 3196insN, 3197insN, 3198insN, 3199insN, 3200insN,
3201insN, 3202insN, 3203insN, 3204insN, 3205insN, 3206insN, 3207insN, 3208insN,
3209insN, 3210insN, 3211insN, 3212insN, 3213insN, 3214insN, 3215insN, 3216insN,
3217insN, 3218insN, 3219insN, 3220insN, 3221insN, 3222insN, 3223insN, 3224insN,
3225insN, 3226insN, 3227insN, 3228insN, 3229insN, 3230insN, 3231insN, 3232insN,
3233insN, 3234insN, 3235insN, 3236insN, 3237insN, 3238insN, 3239insN, 3240insN,
3241insN, 3242insN, 3243insN, 3244insN, 3245insN, 3246insN, 3247insN, 3248insN,
3249insN, 3250insN, 3251insN, 3252insN, 3253insN, 3254insN, 3255insN, 3256insN,
3257insN, 3258insN, 3259insN, 3260insN, 3261insN, 3262insN, 3263insN, 3264insN,
3265insN, 3266insN, 3267insN, 3268insN, 3269insN, 3270insN, 3271insN, 3272insN,
3273insN, 3274insN, 3275insN, 3276insN, 3277insN, 3278insN, 3279insN, 3280insN,
3281insN, 3282insN, 3283insN, 3284insN, 3285insN, 3286insN, 3287insN, 3288insN,
3289insN, 3290insN, 3291insN, 3292insN, 3293insN, 3294insN, 3295insN, 3296insN,
3297insN, 3298insN, 3299insN, 3300insN, 3301insN, 3302insN, 3303insN, 3304insN,
3305insN, 3306insN, 3307insN, 3308insN, 3309insN, 3310insN, 3311insN, 3312insN,
3313insN, 3314insN, 3315insN, 3316insN, 3317insN, 3318insN, 3319insN, 3320insN,
3321insN, 3322insN, 3323insN, 3324insN, 3325insN, 3326insN, 3327insN, 3328insN,
3329insN, 3330insN, 3331insN, 3332insN, 3333insN, 3334insN, 3335insN, 3336insN,
3337insN, 3338insN, 3339insN, 3340insN, 3341insN, 3342insN, 3343insN, 3344insN,
3345insN, 3346insN, 3347insN, 3348insN, 3349insN, 3350insN, 3351insN, 3352insN,
3353insN, 3354insN, 3355insN, 3356insN, 3357insN, 3358insN, 3359insN, 3360insN,
3361insN, 3362insN, 3363insN, 3364insN, 3365insN, 3366insN, 3367insN, 3368insN,
3369insN, 3370insN, 3371insN, 3372insN, 3373insN, 3374insN, 3375insN, 3376insN,
3377insN, 3378insN, 3379insN, 3380insN, 3381insN, 3382insN, 3383insN, 3384insN,
3385insN, 3386insN, 3387insN, 3388insN, 3389insN, 3390insN, 3391insN, 3392insN,
3393insN, 3394insN, 3395insN, 3396insN, 3397insN, 3398insN, 3399insN, 3400insN,
3401insN, 3402insN, 3403insN, 3404insN, 3405insN, 3406insN, 3407insN, 3408insN,
3409insN, 3410insN, 3411insN, 3412insN, 3413insN, 3414insN, 3415insN, 3416insN,
3417insN, 3418insN, 3419insN, 3420insN, 3421insN, 3422insN, 3423insN, 3424insN,
3425insN, 3426insN, 3427insN, 3428insN, 3429insN, 3430insN, 3431insN, 3432insN,
3433insN, 3434insN, 3435insN, 3436insN, 3437insN, 3438insN, 3439insN, 3440insN,
3441insN, 3442insN, 3443insN, 3444insN, 3445insN, 3446insN, 3447insN, 3448insN,
3449insN, 3450insN, 3451insN, 3452insN, 3453insN, 3454insN, 3455insN, 3456insN,
3457insN, 3458insN, 3459insN, 3460insN, 3461insN, 3462insN, 3463insN, 3464insN,
3465insN, 3466insN, 3467insN, 3468insN, 3469insN, 3470insN, 3471insN, 3472insN,
3473insN, 3474insN, 3475insN, 3476insN, 3477insN, 3478insN, 3479insN, 3480insN,
3481insN, 3482insN, 3483insN, 3484insN, 3485insN, 3486insN, 3487insN, 3488insN,
3489insN, 3490insN, 3491insN, 3492insN, 3493insN, 3494insN, 3495insN, 3496insN,
3497insN, 3498insN, 3499insN, 3500insN, 3501insN, 3502insN, 3503insN, 3504insN,
3505insN, 3506insN, 3507insN, 3508insN, 3509insN, 3510insN, 3511insN, 3512insN,
3513insN, 3514insN, 3515insN, 3516insN, 3517insN, 3518insN, 3519insN, 3520insN,
3521insN, 3522insN, 3523insN, 3524insN, 3525insN, 3526insN, 3527insN, 3528insN,
3529insN, 3530insN, 3531insN, 3532insN, 3533insN, 3534insN, 3535insN, 3536insN,
3537insN, 3538insN, 3539insN, 3540insN, 3541insN, 3542insN, 3543insN, 3544insN,
3545insN, 3546insN, 3547insN, 3548insN, 3549insN, 3550insN, 3551insN, 3552insN,
3553insN, 3554insN, 3555insN, 3556insN, 3557insN, 3558insN, 3559insN, 3560insN,
3561insN, 3562insN, 3563insN, 3564insN, 3565insN, 3566insN, 3567insN, 3568insN,
3569insN, 3570insN, 3571insN, 3572insN, 3573insN, 3574insN, 3575insN, 3576insN,
3577insN, 3578insN, 3579insN, 3580insN, 3581insN, 3582insN, 3583insN, 3584insN,
3585insN, 3586insN, 3587insN, 3588insN, 3589insN, 3590insN, 3591insN, 3592insN,
3593insN, 3594insN, 3595insN, 3596insN, 3597insN, 3598insN, 3599insN, 3600insN,
3601insN, 3602insN, 3603insN, 3604insN, 3605insN, 3606insN, 3607insN, 3608insN,
3609insN, 3610insN, 3611insN, 3612insN, 3613insN, 3614insN, 3615insN, 3616insN,
3617insN, 3618insN, 3619insN, 3620insN, 3621insN, 3622insN, 3623insN, 3624insN,
3625insN, 3626insN, 3627insN, 3628insN, 3629insN, 3630insN, 3631insN, 3632insN,
3633insN, 3634insN, 3635insN, 3636insN, 3637insN, 3638insN, 3639insN, 3640insN,
3641insN, 3642insN, 3643insN, 3644insN, 3645insN, 3646insN, 3647insN, 3648insN,
3649insN, 3650insN, 3651insN, 3652insN, 3653insN, 3654insN, 3655insN, 3656insN,
3657insN, 3658insN, 3659insN, 3660insN, 3661insN, 3662insN, 3663insN, 3664insN,
3665insN, 3666insN, 3667insN, 3668insN, 3669insN, 3670insN, 3671insN, 3672insN,
3673insN, 3674insN, 3675insN, 3676insN, 3677insN, 3678insN, 3679insN, 3680insN,
3681insN, 3682insN, 3683insN, 3684insN, 3685insN, 3686insN, 3687insN, 3688insN,
3689insN, 3690insN, 3691insN, 3692insN, 3693insN, 3694insN, 3695insN, 3696insN,
3697insN, 3698insN, 3699insN, 3700insN, 3701insN, 3702insN, 3703insN, 3704insN,
3705insN, 3706insN, 3707insN, 3708insN, 3709insN, 3710insN, 3711insN, 3712insN,
3713insN, 3714insN, 3715insN, 3716insN, 3717insN, 3718insN, 3719insN, 3720insN,
3721insN, 3722insN, 3723insN, 3724insN, 3725insN, 3726insN, 3727insN, 3728insN,
3729insN, 3730insN, 3731insN, 3732insN, 3733insN, 3734insN, 3735insN, 3736insN,
3737insN, 3738insN, 3739insN, 3740insN, 3741insN, 3742insN, 3743insN, 3744insN,
3745insN, 3746insN, 3747insN, 3748insN, 3749insN, 3750insN, 3751insN, 3752insN,
3753insN, 3754insN, 3755insN, 3756insN, 3757insN, 3758insN, 3759insN, 3760insN,
3761insN, 3762insN, 3763insN, 3764insN, 3765insN, 3766insN, 3767insN, 3768insN,
3769insN, 3770insN, 3771insN, 3772insN, 3773insN, 3774insN, 3775insN, 3776insN,
3777insN, 3778insN, 3779insN, 3780insN, 3781insN, 3782insN, 3783insN, 3784insN,
3785insN, 3786insN, 3787insN, 3788insN, 3789insN, 3790insN, 3791insN, 3792insN,
3793insN, 3794insN, 3795insN, 3796insN, 3797insN, 3798insN, 3799insN, 3800insN,
3801insN, 3802insN, 3803insN, 3804insN, 3805insN, 3806insN, 3807insN, 3808insN,
3809insN, 3810insN, 3811insN, 3812insN, 3813insN, 3814insN, 3815insN, 3816insN,
3817insN, 3818insN, 3819insN, 3820insN, 3821insN, 3822insN, 3823insN, 3824insN,
3825insN, 3826insN, 3827insN, 3828insN, 3829insN, 3830insN, 3831insN, 3832insN,
3833insN, 3834insN, 3835insN, 3836insN, 3837insN, 3838insN, 3839insN, 3840insN,
3841insN, 3842insN, 3843insN, 3844insN, 3845insN, 3846insN, 3847insN, 3848insN,
3849insN, 3850insN, 3851insN, 3852insN, 3853insN, 3854insN, 3855insN, 3856insN,
3857insN, 3858insN, 3859insN, 3860insN, 3861insN, 3862insN, 3863insN, 3864insN,
3865insN, 3866insN, 3867insN, 3868insN, 3869insN, 3870insN, 3871insN, 3872insN,
3873insN, 3874insN, 3875insN, 3876insN, 3877insN, 3878insN, 3879insN, 3880insN,
3881insN, 3882insN, 3883insN, 3884insN, 3885insN, 3886insN, 3887insN, 3888insN,
3889insN, 3890insN, 3891insN, 3892insN, 3893insN, 3894insN, 3895insN, 3896insN,
3897insN, 3898insN, 3899insN, 3900insN, 3901insN, 3902insN, 3903insN, 3904insN,
3905insN, 3906insN, 3907insN, 3908insN, 3909insN, 3910insN, 3911insN, 3912insN,
3913insN, 3914insN, 3915insN, 3916insN, 3917insN, 3918insN, 3919insN, 3920insN,
3921insN, 3922insN, 3923insN, 3924insN, 3925insN, 3926insN, 3927insN, 3928insN,
3929insN, 3930insN, 3931insN, 3932insN, 3933insN, 3934insN, 3935insN, 3936insN,
3937insN, 3938insN, 3939insN, 3940insN, 3941insN, 3942insN, 3943insN, 3944insN,
3945insN, 3946insN, 3947insN, 3948insN, 3949insN, 3950insN, 3951insN, 3952insN,
3953insN, 3954insN, 3955insN, 3956insN, 3957insN, 3958insN, 3959insN, 3960insN,
3961insN, 3962insN, 3963insN, 3964insN, 3965insN, 3966insN, 3967insN, 3968insN,
3969insN, 3970insN, 3971insN, 3972insN, 3973insN, 3974insN, 3975insN, 3976insN,
3977insN, 3978insN, 3979insN, 3980insN, 3981insN, 3982insN, 3983insN, 3984insN,
3985insN, 3986insN, 3987insN, 3988insN, 3989insN, 3990insN, 3991insN, 3992insN,
3993insN, 3994insN, 3995insN, 3996insN, 3997insN, 3998insN, 3999insN, 4000insN,
4001insN, 4002insN, 4003insN, 4004insN, 4005insN, 4006insN, 4007insN, 4008insN,
4009insN, 4010insN, 4011insN, 4012insN, 4013insN, 4014insN, 4015insN, 4016insN,
4017insN, 4018insN, 4019insN, 4020insN, 4021insN, 4022insN, 4023insN, 4024insN,
4025insN, 4026insN, 4027insN, 4028insN, 4029insN, 4030insN, 4031insN, 4032insN,
4033insN, 4034insN, 4035insN, 4036insN, 4037insN, 4038insN, 4039insN, 4040insN,
4041insN, 4042insN, 4043insN, 4044insN, 4045insN, 4046insN, 4047insN, 4048insN,
4049insN, 4050insN, 4051insN, 4052insN, 4053insN, 4054insN, 4055insN, 4056insN,
4057insN, 4058insN, 4059insN, 4060insN, 4061insN, 4062insN, 4063insN, 4064insN,
4065insN, 4066insN, 4067insN, 4068insN, 4069insN, 4070insN, 4071insN, 4072insN,
4073insN, 4074insN, 4075insN, 4076insN, 4077insN, 4078insN, 4079insN, 4080insN,
4081insN, 4082insN, 4083insN, 4084insN, 4085insN, 4086insN, 4087insN, 4088insN,
4089insN, 4090insN, 4091insN, 4092insN, 4093insN, 4094insN, 4095insN, 4096insN,
4097insN, 4098insN, 4099insN, 4100insN, 4101insN, 4102insN, 4103insN, 4104insN,
4105insN, 4106insN, 4107insN, 4108insN, 4109insN, 4110insN, 4111insN, 4112insN,
4113insN, 4114insN, 4115insN, 4116insN, 4117insN, 4118insN, 4119insN, 4120insN,
4121insN, 4122insN, 4123insN, 4124insN, 4125insN, 4126insN, 4127insN, 4128insN,
4129insN, 4130insN, 4131insN, 4132insN, 4133insN, 4134insN, 4135insN, 4136insN,
4137insN, 4138insN, 4139insN, 4140insN, 4141insN, 4142insN, 4143insN, 4144insN,
4145insN, 4146insN, 4147insN, 4148insN, 4149insN, 4150insN, 4151insN, 4152insN,
4153insN, 4154insN, 4155insN, 4156insN, 4157insN, 4158insN, 4159insN, 4160insN,
4161insN, 4162insN, 4163insN, 4164insN, 4165insN, 4166insN, 4167insN, 4168insN,
4169insN, 4170insN, 4171insN, 4172insN, 4173insN, 4174insN, 4175insN, 4176insN,
4177insN, 4178insN, 4179insN, 4180insN, 4181insN, 4182insN, 4183insN, 4184insN,
4185insN, 4186insN, 4187insN, 4188insN, 4189insN, 4190insN, 4191insN, 4192insN,
4193insN, 4194insN, 4195insN, 4196insN, 4197insN, 4198insN, 4199insN, 4200insN,
4201insN, 4202insN, 4203insN, 4204insN, 4205insN, 4206insN, 4207insN, 4208insN,
4209insN, 4210insN, 4211insN, 4212insN, 4213insN, 4214insN, 4215insN, 4216insN,
4217insN, 4218insN, 4219insN, 4220insN, 4221insN, 4222insN, 4223insN, 4224insN,
4225insN, 4226insN, 4227insN, 4228insN, 4229insN, 4230insN, 4231insN, 4232insN,
4233insN, 4234insN, 4235insN, 4236insN, 4237insN, 4238insN, 4239insN, 4240insN,
4241insN, 4242insN, 4243insN, 4244insN, 4245insN, 4246insN, 4247insN, 4248insN,
4249insN, 4250insN, 4251insN, 4252insN, 4253insN, 4254insN, 4255insN, 4256insN,
4257insN, 4258insN, 4259insN, 4260insN, 4261insN, 4262insN, 4263insN, 4264insN,
4265insN, 4266insN, 4267insN, 4268insN, 4269insN, 4270insN, 4271insN, 4272insN,
4273insN, 4274insN, 4275insN, 4276insN, 4277insN, 4278insN, 4279insN, 4280insN,
4281insN, 4282insN, 4283insN, 4284insN, 4285insN, 4286insN, 4287insN, 4288insN,
4289insN, 4290insN, 4291insN, 4292insN, 4293insN, 4294insN, 4295insN, 4296insN,
4297insN, 4298insN, 4299insN, 4300insN, 4301insN, 4302insN, 4303insN, 4304insN,
4305insN, 4306insN, 4307insN, 4308insN, 4309insN, 4310insN, 4311insN, 4312insN,
4313insN, 4314insN, 4315insN, 4316insN, 4317insN, 4318insN, 4319insN, 4320insN,
4321insN, 4322insN, 4323insN, 4324insN, 4325insN, 4326insN, 4327insN, 4328insN,
4329insN, 4330insN, 4331insN, 4332insN, 4333insN, 4334insN, 4335insN, 4336insN,
4337insN, 4338insN, 4339insN, 4340insN, 4341insN, 4342insN, 4343insN, 4344insN,
4345insN, 4346insN, 4347insN, 4348insN, 4349insN, 4350insN, 4351insN, 4352insN,
4353insN, 4354insN, 4355insN, 4356insN, 4357insN, 4358insN, 4359insN, 4360insN,
4361insN, 4362insN, 4363insN, 4364insN, 4365insN, 4366insN, 4367insN, 4368insN,
4369insN, 4370insN, 4371insN, 4372insN, 4373insN, 4374insN, 4375insN, 4376insN,
4377insN, 4378insN, 4379insN, 4380insN, 4381insN, 4382insN, 4383insN, 4384insN,
4385insN, 4386insN, 4387insN, 4388insN, 4389insN, 4390insN, 4391insN, 4392insN,
4393insN, 4394insN, 4395insN, 4396insN, 4397insN, 4398insN, 4399insN, 4400insN,
4401insN, 4402insN, 4403insN, 4404insN, 4405insN, 4406insN, 4407insN, 4408insN,
4409insN, 4410insN, 4411insN, 4412insN, 4413insN, 4414insN, 4415insN, 4416insN,
4417insN, 4418insN, 4419insN, 4420insN, 4421insN, 4422insN, 4423insN, 4424insN,
4425insN, 4426insN, 4427insN, 4428insN, 4429insN, 4430insN, 4431insN, 4432insN,
4433insN, 4434insN, 4435insN, 4436insN, 4437insN, 4438insN, 4439insN, 4440insN,
4441insN, 4442insN, 4443insN, 4444insN, 4445insN, 4446insN, 4447insN, 4448insN,
4449insN, 4450insN, 4451insN, 4452insN, 4453insN, 4454insN, 4455insN, 4456insN,
4457insN, 4458insN, 4459insN, 4460insN, 4461insN, 4462insN, 4463insN, 4464insN,
4465insN, 4466insN, 4467insN, 4468insN, 4469insN, 4470insN, 4471insN, 4472insN,
4473insN, 4474insN, 4475insN, 4476insN, 4477insN, 4478insN, 4479insN, 4480insN,
4481insN, 4482insN, 4483insN, 4484insN, 4485insN, 4486insN, 4487insN, 4488insN,
4489insN, 4490insN, 4491insN, 4492insN, 4493insN, 4494insN, 4495insN, 4496insN,
4497insN, 4498insN, 4499insN, 4500insN, 4501insN, 4502insN, 4503insN, 4504insN,
4505insN, 4506insN, 4507insN, 4508insN, 4509insN, 4510insN, 4511insN, 4512insN,
4513insN, 4514insN, 4515insN, 4516insN, 4517insN, 4518insN, 4519insN, 4520insN,
4521insN, 4522insN, 4523insN, 4524insN, 4525insN, 4526insN, 4527insN, 4528insN,
4529insN, 4530insN, 4531insN, 4532insN, 4533insN, 4534insN, 4535insN, 4536insN,
4537insN, 4538insN, 4539insN, 4540insN, 4541insN, 4542insN, 4543insN, 4544insN,
4545insN, 4546insN, 4547insN, 4548insN, 4549insN, 4550insN, 4551insN, 4552insN,
4553insN, 4554insN, 4555insN, 4556insN, 4557insN, 4558insN, 4559insN, 4560insN,
4561insN, 4562insN, 4563insN, 4564insN, 4565insN, 4566insN, 4567insN, 4568insN,
4569insN, 4570insN, 4571insN, 4572insN, 4573insN, 4574insN, 4575insN, 4576insN,
4577insN, 4578insN, 4579insN, 4580insN, 4581insN, 4582insN, 4583insN, 4584insN,
4585insN, 4586insN, 4587insN, 4588insN, 4589insN, 4590insN, 4591insN, 4592insN,
4593insN, 4594insN, 4595insN, 4596insN, 4597insN, 4598insN, 4599insN, 4600insN,
4601insN, 4602insN, 4603insN, 4604insN, 4605insN, 4606insN, 4607insN, 4608insN,
4609insN, 4610insN, 4611insN, 4612insN, 4613insN, 4614insN, 4615insN, 4616insN,
4617insN, 4618insN, 4619insN, 4620insN, 4621insN, 4622insN, 4623insN, 4624insN,
4625insN, 4626insN, 4627insN, 4628insN, 4629insN, 4630insN, 4631insN, 4632insN,
4633insN, 4634insN, 4635insN, 4636insN, 4637insN, 4638insN, 4639insN, 4640insN,
4641insN, 4642insN, 4643insN, 4644insN, 4645insN, 4646insN, 4647insN, 4648insN,
4649insN, 4650insN, 4651insN, 4652insN, 4653insN, 4654insN, 4655insN, 4656insN,
4657insN, 4658insN, 4659insN, 4660insN, 4661insN, 4662insN, 4663insN, 4664insN,
4665insN, 4666insN, 4667insN, 4668insN, 4669insN, 4670insN, 4671insN, 4672insN,
4673insN, 4674insN, 4675insN, 4676insN, 4677insN, 4678insN, 4679insN, 4680insN,
4681insN, 4682insN, 4683insN, 4684insN, 4685insN, 4686insN, 4687insN, 4688insN,
4689insN, 4690insN, 4691insN, 4692insN, 4693insN, 4694insN, 4695insN, 4696insN,
4697insN, 4698insN, 4699insN, 4700insN, 4701insN, 4702insN, 4703insN, 4704insN,
4705insN, 4706insN, 4707insN, 4708insN, 4709insN, 4710insN, 4711insN, 4712insN,
4713insN, 4714insN, 4715insN, 4716insN, 4717insN, 4718insN, 4719insN, 4720insN,
4721insN, 4722insN, 4723insN, 4724insN, 4725insN, 4726insN, 4727insN, 4728insN,
4729insN, 4730insN, 4731insN, 4732insN, 4733insN, 4734insN, 4735insN, 4736insN,
4737insN, 4738insN, 4739insN, 4740insN, 4741insN, 4742insN, 4743insN, 4744insN,
4745insN, 4746insN, 4747insN, 4748insN, 4749insN, 4750insN, 4751insN, 4752insN,
4753insN, 4754insN, 4755insN, 4756insN, 4757insN, 4758insN, 4759insN, 4760insN,
4761insN, 4762insN, 4763insN, 4764insN, 4765insN, 4766insN, 4767insN, 4768insN,
4769insN, 4770insN, 4771insN, 4772insN, 4773insN, 4774insN, 4775insN, 4776insN,
4777insN, 4778insN, 4779insN, 4780insN, 4781insN, 4782insN, 4783insN, 4784insN,
4785insN, 4786insN, 4787insN, 4788insN, 4789insN, 4790insN, 4791insN, 4792insN,
4793insN, 4794insN, 4795insN, 4796insN, 4797insN, 4798insN, 4799insN, 4800insN,
4801insN, 4802insN, 4803insN, 4804insN, 4805insN, 4806insN, 4807insN, 4808insN,
4809insN, 4810insN, 4811insN, 4812insN, 4813insN, 4814insN, 4815insN, 4816insN,
4817insN, 4818insN, 4819insN, 4820insN, 4821insN, 4822insN, 4823insN, 4824insN,
4825insN, 4826insN, 4827insN, 4828insN, 4829insN, 4830insN, 4831insN, 4832insN,
4833insN, 4834insN, 4835insN, 4836insN, 4837insN, 4838insN, 4839insN, 4840insN,
4841insN, 4842insN, 4843insN, 4844insN, 4845insN, 4846insN, 4847insN, 4848insN,
4849insN, 4850insN, 4851insN, 4852insN, 4853insN, 4854insN, 4855insN, 4856insN,
4857insN, 4858insN, 4859insN, 4860insN, 4861insN, 4862insN, 4863insN, 4864insN,
4865insN, 4866insN, 4867insN, 4868insN, 4869insN, 4870insN, 4871insN, 4872insN,
4873insN, 4874insN, 4875insN, 4876insN, 4877insN, 4878insN, 4879insN, 4880insN,
4881insN, 4882insN, 4883insN, 4884insN, 4885insN, 4886insN, 4887insN, 4888insN,
4889insN, 4890insN, 4891insN, 4892insN, 4893insN, 4894insN, 4895insN, 4896insN,
4897insN, 4898insN, 4899insN, 4900insN, 4901insN, 4902insN, 4903insN, 4904insN,
4905insN, 4906insN, 4907insN, 4908insN, 4909insN, 4910insN, 4911insN, 4912insN,
4913insN, 4914insN, 4915insN, 4916insN, 4917insN, 4918insN, 4919insN, 4920insN,
4921insN, 4922insN, 4923insN, 4924insN, 4925insN, 4926insN, 4927insN, 4928insN,
4929insN, 4930insN, 4931insN, 4932insN, 4933insN, 4934insN, 4935insN, 4936insN,
4937insN, 4938insN, 4939insN, 4940insN, 4941insN, 4942insN, 4943insN, 4944insN,
4945insN, 4946insN, 4947insN, 4948insN, 4949insN, 4950insN, 4951insN, 4952insN,
4953insN, 4954insN, 4955insN, 4956insN, 4957insN, 4958insN, 4959insN, 4960insN,
4961insN, 4962insN, 4963insN, 4964insN, 4965insN, 4966insN, 4967insN, 4968insN,
4969insN, 4970insN, 4971insN, 4972insN, 4973insN, 4974insN, 4975insN, 4976insN,
4977insN, 4978insN, 4979insN, 4980insN, 4981insN, 4982insN, 4983insN, 4984insN,
4985insN, 4986insN, 4987insN, 4988insN, 4989insN, 4990insN, 4991insN, 4992insN,
4993insN, 4994insN, 4995insN, 4996insN, 4997insN, 4998insN, 4999insN, 5000insN,
5001insN, 5002insN, 5003insN, 5004insN, 5005insN, 5006insN, 5007insN, 5008insN,
5009insN, 5010insN, 5011insN, 5012insN, 5013insN, 5014insN, 5015insN, 5016insN,
5017insN, 5018insN, 5019insN, 5020insN, 5021insN, 5022insN, 5023insN, 5024insN,
5025insN, 5026insN, 5027insN, 5028insN, 5029insN, 5030insN, 5031insN, 5032insN,
5033insN, 5034insN, 5035insN, 5036insN, 5037insN, 5038insN, 5039insN, 5040insN,
5041insN, 5042insN, 5043insN, 5044insN, 5045insN, 5046insN, 5047insN, 5048insN,
5049insN, 5050insN, 5051insN, 5052insN, 5053insN, 5054insN, 5055insN, 5056insN,
5057insN, 5058insN, 5059insN, 5060insN, 5061insN, 5062insN, 5063insN, 5064insN,
5065insN, 5066insN, 5067insN, 5068insN, 5069insN, 5070insN, 5071insN, 5072insN,
5073insN, 5074insN, 5075insN, 5076insN, 5077insN, 5078insN, 5079insN, 5080insN,
5081insN, 5082insN, 5083insN, 5084insN, 5085insN, 5086insN, 5087insN, 5088insN,
5089insN, 5090insN, 5091insN, 5092insN, 5093insN, 5094insN, 5095insN, 5096insN,
5097insN, 5098insN, 5099insN, 5100insN, 5101insN, 5102insN, 5103insN, 5104insN,
5105insN, 5106insN, 5107insN, 5108insN, 5109insN, 5110insN, 5111insN, 5112insN,
5113insN, 5114insN, 5115insN, 5116insN, 5117insN, 5118insN, 5119insN, 5120insN,
5121insN, 5122insN, 5123insN, 5124insN, 5125insN, 5126insN, 5127insN, 5128insN,
5129insN, 5130insN, 5131insN, 5132insN, 5133insN, 5134insN, 5135insN, 5136insN,
5137insN, 5138insN, 5139insN, 5140insN, 5141insN, 5142insN, 5143insN, 5144insN,
5145insN, 5146insN, 5147insN, 5148insN, 5149insN, 5150insN, 5151insN, 5152insN,
5153insN, 5154insN, 5155insN, 5156insN, 5157insN, 5158insN, 5159insN, 5160insN,
5161insN, 5162insN, 5163insN, 5164insN, 5165insN, 5166insN, 5167insN, 5168insN,
5169insN, 5170insN, 5171insN, 5172insN, 5173insN, 5174insN, 5175insN, 5176insN,
5177insN, 5178insN, 5179insN, 5180insN, 5181insN, 5182insN, 5183insN, 5184insN,
5185insN, 5186insN, 5187insN, 5188insN, 5189insN, 5190insN, 5191insN, 5192insN,
5193insN, 5194insN, 5195insN, 5196insN, 5197insN, 5198insN, 5199insN, 5200insN,
5201insN, 5202insN, 5203insN, 5204insN, 5205insN, 5206insN, 5207insN, 5208insN,
5209insN, 5210insN, 5211insN, 5212insN, 5213insN, 5214insN, 5215insN, 5216insN,
5217insN, 5218insN, 5219insN, 5220insN, 5221insN, 5222insN, 5223insN, 5224insN,
5225insN, 5226insN, 5227insN, 5228insN, 5229insN, 5230insN, 5231insN, 5232insN,
5233insN, 5234insN, 5235insN, 5236insN, 5237insN, 5238insN, 5239insN, 5240insN,
5241insN, 5242insN, 5243insN, 5244insN, 5245insN, 5246insN, 5247insN, 5248insN,
5249insN, 5250insN, 5251insN, 5252insN, 5253insN, 5254insN, 5255insN, 5256insN,
5257insN, 5258insN, 5259insN, 5260insN, 5261insN, 5262insN, 5263insN, 5264insN,
5265insN, 5266insN, 5267insN, 5268insN, 5269insN, 5270insN, 5271insN, 5272insN,
5273insN, 5274insN, 5275insN, 5276insN, 5277insN, 5278insN, 5279insN, 5280insN,
5281insN, 5282insN, 5283insN, 5284insN, 5285insN, 5286insN, 5287insN, 5288insN,
5289insN, 5290insN, 5291insN, 5292insN, 5293insN, 5294insN, 5295insN, 5296insN,
5297insN, 5298insN, 5299insN, 5300insN, 5301insN, 5302insN, 5303insN, 5304insN,
5305insN, 5306insN, 5307insN, 5308insN, 5309insN, 5310insN, 5311insN, 5312insN,
5313insN, 5314insN, 5315insN, 5316insN, 5317insN, 5318insN, 5319insN, 5320insN,
5321insN, 5322insN, 5323insN, 5324insN, 5325insN, 5326insN, 5327insN, 5328insN,
5329insN, 5330insN, 5331insN, 5332insN, 5333insN, 5334insN, 5335insN, 5336insN,
5337insN, 5338insN, 5339insN, 5340insN, 5341insN, 5342insN, 5343insN, 5344insN,
5345insN, 5346insN, 5347insN, 5348insN, 5349insN, 5350insN, 5351insN, 5352insN,
5353insN, 5354insN, 5355insN, 5356insN, 5357insN, 5358insN, 5359insN, 5360insN,
5361insN, 5362insN, 5363insN, 5364insN, 5365insN, 5366insN, 5367insN, 5368insN,
5369insN, 5370insN, 5371insN, 5372insN, 5373insN, 5374insN, 5375insN, 5376insN,
5377insN, 5378insN, 5379insN, 5380insN, 5381insN, 5382insN, 5383insN, 5384insN,
5385insN, 5386insN, 5387insN, 5388insN, 5389insN, 5390insN, 5391insN, 5392insN,
5393insN, 5394insN, 5395insN, 5396insN, 5397insN, 5398insN, 5399insN, 5400insN,
5401insN, 5402insN, 5403insN, 5404insN, 5405insN, 5406insN, 5407insN, 5408insN,
5409insN, 5410insN, 5411insN, 5412insN, 5413insN, 5414insN, 5415insN, 5416insN,
5417insN, 5418insN, 5419insN, 5420insN, 5421insN, 5422insN, 5423insN, 5424insN,
5425insN, 5426insN, 5427insN, 5428insN, 5429insN, 5430insN, 5431insN, 5432insN,
5433insN, 5434insN, 5435insN, 5436insN, 5437insN, 5438insN, 5439insN, 5440insN,
5441insN, 5442insN, 5443insN, 5444insN, 5445insN, 5446insN, 5447insN, 5448insN,
5449insN, 5450insN, 5451insN, 5452insN, 5453insN, 5454insN, 5455insN, 5456insN,
5457insN, 5458insN, 5459insN, 5460insN, 5461insN, 5462insN, 5463insN, 5464insN,
5465insN, 5466insN, 5467insN, 5468insN, 5469insN, 5470insN, 5471insN, 5472insN,
5473insN, 5474insN, 5475insN, 5476insN, 5477insN, 5478insN, 5479insN, 5480insN,
5481insN, 5482insN, 5483insN, 5484insN, 5485insN, 5486insN, 5487insN, 5488insN,
5489insN, 5490insN, 5491insN, 5492insN, 5493insN, 5494insN, 5495insN, 5496insN,
5497insN, 5498insN, 5499insN, 5500insN, 5501insN, 5502insN, 5503insN, 5504insN,
5505insN, 5506insN, 5507insN, 5508insN, 5509insN, 5510insN, 5511insN, 5512insN,
5513insN, 5514insN, 5515insN, 5516insN, 5517insN, 5518insN, 5519insN, 5520insN,
5521insN, 5522insN, 5523insN, 5524insN, 5525insN, 5526insN, 5527insN, 5528insN,
5529insN, 5530insN, 5531insN, 5532insN, 5533insN, 5534insN, 5535insN, 5536insN,
5537insN, 5538insN, 5539insN, 5540insN, 5541insN, 5542insN, 5543insN, 5544insN,
5545insN, 5546insN, 5547insN, 5548insN, 5549insN, 5550insN, 5551insN, 5552insN,
5553insN, 5554insN, 5555insN, 5556insN, 5557insN, 5558insN, 5559insN, 5560insN,
5561insN, 5562insN, 5563insN, 5564insN, 5565insN, 5566insN, 5567insN, 5568insN,
5569insN, 5570insN, 5571insN, 5572insN, 5573insN, 5574insN, 5575insN, 5576insN,
5577insN, 5578insN, 5579insN, 5580insN, 5581insN, 5582insN, 5583insN, 5584insN,
5585insN, 5586insN, 5587insN, 5588insN, 5589insN, 5590insN, 5591insN, 5592insN,
5593insN, 5594insN, 5595insN, 5596insN, 5597insN, 5598insN, 5599insN, 5600insN,
5601insN, 5602insN, 5628insN, 5629insN, 5652insN, 5653insN, 5658insN, 5659insN,
5676insN, 5677insN, 5706insN, 5707insN.

[0230]

TABLE 12
List of Two Base Insertions
(NN = AA, AT, AG, AC, TA, TT, TG, TC, GA, GT, GG, GC, CA, CT, CG, or CC)
122insNN, 123insNN, 124insNN, 125insNN, 126insNN, 127insNN, 128insNN, 129insNN,
130insNN, 131insNN, 132insNN, 133insNN, 134insNN, 135insNN, 136insNN, 137insNN,
138insNN, 139insNN, 140insNN, 141insNN, 142insNN, 143insNN, 144insNN, 145insNN,
146insNN, 147insNN, 148insNN, 149insNN, 150insNN, 151insNN, 152insNN, 153insNN,
154insNN, 155insNN, 156insNN, 157insNN, 158insNN, 159insNN, 160insNN, 161insNN,
162insNN, 163insNN, 164insNN, 165insNN, 166insNN, 167insNN, 168insNN, 169insNN,
170insNN, 171insNN, 172insNN, 173insNN, 174insNN, 175insNN, 176insNN, 177insNN,
178insNN, 179insNN, 180insNN, 181insNN, 182insNN, 183insNN, 184insNN, 185insNN,
186insNN, 187insNN, 188insNN, 189insNN, 190insNN, 191insNN, 192insNN, 193insNN,
194insNN, 195insNN, 196insNN, 197insNN, 198insNN, 199insNN, 200insNN, 201insNN,
202insNN, 203insNN, 204insNN, 205insNN, 206insNN, 207insNN, 208insNN, 209insNN,
210insNN, 211insNN, 212insNN, 213insNN, 214insNN, 215insNN, 216insNN, 217insNN,
218insNN, 219insNN, 220insNN, 221insNN, 222insNN, 223insNN, 224insNN, 225insNN,
226insNN, 227insNN, 228insNN, 229insNN, 230insNN, 231insNN, 232insNN, 233insNN,
234insNN, 235insNN, 236insNN, 237insNN, 238insNN, 239insNN, 240insNN, 241insNN,
242insNN, 243insNN, 244insNN, 245insNN, 246insNN, 247insNN, 248insNN, 249insNN,
250insNN, 251insNN, 252insNN, 253insNN, 254insNN, 255insNN, 256insNN, 257insNN,
258insNN, 259insNN, 260insNN, 261insNN, 262insNN, 263insNN, 264insNN, 265insNN,
266insNN, 267insNN, 268insNN, 269insNN, 270insNN, 271insNN, 272insNN, 273insNN,
274insNN, 275insNN, 276insNN, 277insNN, 278insNN, 279insNN, 280insNN, 281insNN,
282insNN, 283insNN, 284insNN, 285insNN, 286insNN, 287insNN, 288insNN, 289insNN,
290insNN, 291insNN, 292insNN, 293insNN, 294insNN, 295insNN, 296insNN, 297insNN,
298insNN, 299insNN, 300insNN, 301insNN, 302insNN, 303insNN, 304insNN, 305insNN,
306insNN, 307insNN, 308insNN, 309insNN, 310insNN, 311insNN, 312insNN, 313insNN,
314insNN, 315insNN, 316insNN, 317insNN, 318insNN, 319insNN, 320insNN, 321insNN,
322insNN, 323insNN, 324insNN, 325insNN, 326insNN, 327insNN, 328insNN, 329insNN,
330insNN, 331insNN, 332insNN, 333insNN, 334insNN, 335insNN, 336insNN, 337insNN,
338insNN, 339insNN, 340insNN, 341insNN, 342insNN, 343insNN, 344insNN, 345insNN,
346insNN, 347insNN, 348insNN, 349insNN, 350insNN, 351insNN, 352insNN, 353insNN,
354insNN, 355insNN, 356insNN, 357insNN, 358insNN, 359insNN, 360insNN, 361insNN,
362insNN, 363insNN, 364insNN, 365insNN, 366insNN, 367insNN, 368insNN, 369insNN,
370insNN, 371insNN, 372insNN, 373insNN, 374insNN, 375insNN, 376insNN, 377insNN,
378insNN, 379insNN, 380insNN, 381insNN, 382insNN, 383insNN, 384insNN, 385insNN,
386insNN, 387insNN, 388insNN, 389insNN, 390insNN, 391insNN, 392insNN, 393insNN,
394insNN, 395insNN, 396insNN, 397insNN, 398insNN, 399insNN, 400insNN, 401insNN,
402insNN, 403insNN, 404insNN, 405insNN, 406insNN, 407insNN, 408insNN, 409insNN,
410insNN, 411insNN, 412insNN, 413insNN, 414insNN, 415insNN, 416insNN, 417insNN,
418insNN, 419insNN, 420insNN, 421insNN, 422insNN, 423insNN, 424insNN, 425insNN,
426insNN, 427insNN, 428insNN, 429insNN, 430insNN, 431insNN, 432insNN, 433insNN,
434insNN, 435insNN, 436insNN, 437insNN, 438insNN, 439insNN, 440insNN, 441insNN,
442insNN, 443insNN, 444insNN, 445insNN, 446insNN, 447insNN, 448insNN, 449insNN,
450insNN, 451insNN, 452insNN, 453insNN, 454insNN, 455insNN, 456insNN, 457insNN,
458insNN, 459insNN, 460insNN, 461insNN, 462insNN, 463insNN, 464insNN, 465insNN,
466insNN, 467insNN, 468insNN, 469insNN, 470insNN, 471insNN, 472insNN, 473insNN,
474insNN, 475insNN, 476insNN, 477insNN, 478insNN, 479insNN, 480insNN, 481insNN,
482insNN, 483insNN, 484insNN, 485insNN, 486insNN, 487insNN, 488insNN, 489insNN,
490insNN, 491insNN, 492insNN, 493insNN, 494insNN, 495insNN, 496insNN, 497insNN,
498insNN, 499insNN, 500insNN, 501insNN, 502insNN, 503insNN, 504insNN, 505insNN,
506insNN, 507insNN, 508insNN, 509insNN, 510insNN, 511insNN, 512insNN, 513insNN,
514insNN, 515insNN, 516insNN, 517insNN, 518insNN, 519insNN, 520insNN, 521insNN,
522insNN, 523insNN, 524insNN, 525insNN, 526insNN, 527insNN, 528insNN, 529insNN,
530insNN, 531insNN, 532insNN, 533insNN, 534insNN, 535insNN, 536insNN, 537insNN,
538insNN, 539insNN, 540insNN, 541insNN, 542insNN, 543insNN, 544insNN, 545insNN,
546insNN, 547insNN, 548insNN, 549insNN, 550insNN, 551insNN, 552insNN, 553insNN,
554insNN, 555insNN, 556insNN, 557insNN, 558insNN, 559insNN, 560insNN, 561insNN,
562insNN, 563insNN, 564insNN, 565insNN, 566insNN, 567insNN, 568insNN, 569insNN,
570insNN, 571insNN, 572insNN, 573insNN, 574insNN, 575insNN, 576insNN, 577insNN,
578insNN, 579insNN, 580insNN, 581insNN, 582insNN, 583insNN, 584insNN, 585insNN,
586insNN, 587insNN, 588insNN, 589insNN, 590insNN, 591insNN, 592insNN, 593insNN,
594insNN, 595insNN, 596insNN, 597insNN, 598insNN, 599insNN, 600insNN, 601insNN,
602insNN, 603insNN, 604insNN, 605insNN, 606insNN, 607insNN, 608insNN, 609insNN,
610insNN, 611insNN, 612insNN, 613insNN, 614insNN, 615insNN, 616insNN, 617insNN,
618insNN, 619insNN, 620insNN, 621insNN, 622insNN, 623insNN, 624insNN, 625insNN,
626insNN, 627insNN, 628insNN, 629insNN, 630insNN, 631insNN, 632insNN, 633insNN,
634insNN, 635insNN, 636insNN, 637insNN, 638insNN, 639insNN, 640insNN, 641insNN,
642insNN, 643insNN, 644insNN, 645insNN, 646insNN, 647insNN, 648insNN, 649insNN,
650insNN, 651insNN, 652insNN, 653insNN, 654insNN, 655insNN, 656insNN, 657insNN,
658insNN, 659insNN, 660insNN, 661insNN, 662insNN, 663insNN, 664insNN, 665insNN,
666insNN, 667insNN, 668insNN, 669insNN, 670insNN, 671insNN, 672insNN, 673insNN,
674insNN, 675insNN, 676insNN, 677insNN, 678insNN, 679insNN, 680insNN, 681insNN,
682insNN, 683insNN, 684insNN, 685insNN, 686insNN, 687insNN, 688insNN, 689insNN,
690insNN, 691insNN, 692insNN, 693insNN, 694insNN, 695insNN, 696insNN, 697insNN,
698insNN, 699insNN, 700insNN, 701insNN, 702insNN, 703insNN, 704insNN, 705insNN,
706insNN, 707insNN, 708insNN, 709insNN, 710insNN, 711insNN, 712insNN, 713insNN,
714insNN, 715insNN, 716insNN, 717insNN, 718insNN, 719insNN, 720insNN, 721insNN,
722insNN, 723insNN, 724insNN, 725insNN, 726insNN, 727insNN, 728insNN, 729insNN,
730insNN, 731insNN, 732insNN, 733insNN, 734insNN, 735insNN, 736insNN, 737insNN,
738insNN, 739insNN, 740insNN, 741insNN, 742insNN, 743insNN, 744insNN, 745insNN,
746insNN, 747insNN, 748insNN, 749insNN, 750insNN, 751insNN, 752insNN, 753insNN,
754insNN, 755insNN, 756insNN, 757insNN, 758insNN, 759insNN, 760insNN, 761insNN,
762insNN, 763insNN, 764insNN, 765insNN, 766insNN, 767insNN, 768insNN, 769insNN,
770insNN, 771insNN, 772insNN, 773insNN, 774insNN, 775insNN, 776insNN, 777insNN,
778insNN, 779insNN, 780insNN, 781insNN, 782insNN, 783insNN, 784insNN, 785insNN,
786insNN, 787insNN, 788insNN, 789insNN, 790insNN, 791insNN, 792insNN, 793insNN,
794insNN, 795insNN, 796insNN, 797insNN, 798insNN, 799insNN, 800insNN, 801insNN,
802insNN, 803insNN, 804insNN, 805insNN, 806insNN, 807insNN, 808insNN, 809insNN,
810insNN, 811insNN, 812insNN, 813insNN, 814insNN, 815insNN, 816insNN, 817insNN,
818insNN, 819insNN, 820insNN, 821insNN, 822insNN, 823insNN, 824insNN, 825insNN,
826insNN, 827insNN, 828insNN, 829insNN, 830insNN, 831insNN, 832insNN, 833insNN,
834insNN, 835insNN, 836insNN, 837insNN, 838insNN, 839insNN, 840insNN, 841insNN,
842insNN, 843insNN, 844insNN, 845insNN, 846insNN, 847insNN, 848insNN, 849insNN,
850insNN, 851insNN, 852insNN, 853insNN, 854insNN, 855insNN, 856insNN, 857insNN,
858insNN, 859insNN, 860insNN, 861insNN, 862insNN, 863insNN, 864insNN, 865insNN,
866insNN, 867insNN, 868insNN, 869insNN, 870insNN, 871insNN, 872insNN, 873insNN,
874insNN, 875insNN, 876insNN, 877insNN, 878insNN, 879insNN, 880insNN, 881insNN,
882insNN, 883insNN, 884insNN, 885insNN, 886insNN, 887insNN, 888insNN, 889insNN,
890insNN, 891insNN, 892insNN, 893insNN, 894insNN, 895insNN, 896insNN, 897insNN,
898insNN, 899insNN, 900insNN, 901insNN, 902insNN, 903insNN, 904insNN, 905insNN,
906insNN, 907insNN, 908insNN, 909insNN, 910insNN, 911insNN, 912insNN, 913insNN,
914insNN, 915insNN, 916insNN, 917insNN, 918insNN, 919insNN, 920insNN, 921insNN,
922insNN, 923insNN, 924insNN, 925insNN, 926insNN, 927insNN, 928insNN, 929insNN,
930insNN, 931insNN, 932insNN, 933insNN, 934insNN, 935insNN, 936insNN, 937insNN,
938insNN, 939insNN, 940insNN, 941insNN, 942insNN, 943insNN, 944insNN, 945insNN,
946insNN, 947insNN, 948insNN, 949insNN, 950insNN, 951insNN, 952insNN, 953insNN,
954insNN, 955insNN, 956insNN, 957insNN, 958insNN, 959insNN, 960insNN, 961insNN,
962insNN, 963insNN, 964insNN, 965insNN, 966insNN, 967insNN, 968insNN, 969insNN,
970insNN, 971insNN, 972insNN, 973insNN, 974insNN, 975insNN, 976insNN, 977insNN,
978insNN, 979insNN, 980insNN, 981insNN, 982insNN, 983insNN, 984insNN, 985insNN,
986insNN, 987insNN, 988insNN, 989insNN, 990insNN, 991insNN, 992insNN, 993insNN,
994insNN, 995insNN, 996insNN, 997insNN, 998insNN, 999insNN, 1000insNN, 1001insNN,
1002insNN, 1003insNN, 1004insNN, 1005insNN, 1006insNN, 1007insNN, 1008insNN,
1009insNN, 1010insNN, 1011insNN, 1012insNN, 1013insNN, 1014insNN, 1015insNN,
1016insNN, 1017insNN, 1018insNN, 1019insNN, 1020insNN, 1021insNN, 1022insNN,
1023insNN, 1024insNN, 1025insNN, 1026insNN, 1027insNN, 1028insNN, 1029insNN,
1030insNN, 1031insNN, 1032insNN, 1033insNN, 1034insNN, 1035insNN, 1036insNN,
1037insNN, 1038insNN, 1039insNN, 1040insNN, 1041insNN, 1042insNN, 1043insNN,
1044insNN, 1045insNN, 1046insNN, 1047insNN, 1048insNN, 1049insNN, 1050insNN,
1051insNN, 1052insNN, 1053insNN, 1054insNN, 1055insNN, 1056insNN, 1057insNN,
1058insNN, 1059insNN, 1060insNN, 1061insNN, 1062insNN, 1063insNN, 1064insNN,
1065insNN, 1066insNN, 1067insNN, 1068insNN, 1069insNN, 1070insNN, 1071insNN,
1072insNN, 1073insNN, 1074insNN, 1075insNN, 1076insNN, 1077insNN, 1078insNN,
1079insNN, 1080insNN, 1081insNN, 1082insNN, 1083insNN, 1084insNN, 1085insNN,
1086insNN, 1087insNN, 1088insNN, 1089insNN, 1090insNN, 1091insNN, 1092insNN,
1093insNN, 1094insNN, 1095insNN, 1096insNN, 1097insNN, 1098insNN, 1099insNN,
1100insNN, 1101insNN, 1102insNN, 1103insNN, 1104insNN, 1105insNN, 1106insNN,
1107insNN, 1108insNN, 1109insNN, 1110insNN, 1111insNN, 1112insNN, 1113insNN,
1114insNN, 1115insNN, 1116insNN, 1117insNN, 1118insNN, 1119insNN, 1120insNN,
1121insNN, 1122insNN, 1123insNN, 1124insNN, 1125insNN, 1126insNN, 1127insNN,
1128insNN, 1129insNN, 1130insNN, 1131insNN, 1132insNN, 1133insNN, 1134insNN,
1135insNN, 1136insNN, 1137insNN, 1138insNN, 1139insNN, 1140insNN, 1141insNN,
1142insNN, 1143insNN, 1144insNN, 1145insNN, 1146insNN, 1147insNN, 1148insNN,
1149insNN, 1150insNN, 1151insNN, 1152insNN, 1153insNN, 1154insNN, 1155insNN,
1156insNN, 1157insNN, 1158insNN, 1159insNN, 1160insNN, 1161insNN, 1162insNN,
1163insNN, 1164insNN, 1165insNN, 1166insNN, 1167insNN, 1168insNN, 1169insNN,
1170insNN, 1171insNN, 1172insNN, 1173insNN, 1174insNN, 1175insNN, 1176insNN,
1177insNN, 1178insNN, 1179insNN, 1180insNN, 1181insNN, 1182insNN, 1183insNN,
1184insNN, 1185insNN, 1186insNN, 1187insNN, 1188insNN, 1189insNN, 1190insNN,
1191insNN, 1192insNN, 1193insNN, 1194insNN, 1195insNN, 1196insNN, 1197insNN,
1198insNN, 1199insNN, 1200insNN, 1201insNN, 1202insNN, 1203insNN, 1204insNN,
1205insNN, 1206insNN, 1207insNN, 1208insNN, 1209insNN, 1210insNN, 1211insNN,
1212insNN, 1213insNN, 1214insNN, 1215insNN, 1216insNN, 1217insNN, 1218insNN,
1219insNN, 1220insNN, 1221insNN, 1222insNN, 1223insNN, 1224insNN, 1225insNN,
1226insNN, 1227insNN, 1228insNN, 1229insNN, 1230insNN, 1231insNN, 1232insNN,
1233insNN, 1234insNN, 1235insNN, 1236insNN, 1237insNN, 1238insNN, 1239insNN,
1240insNN, 1241insNN, 1242insNN, 1243insNN, 1244insNN, 1245insNN, 1246insNN,
1247insNN, 1248insNN, 1249insNN, 1250insNN, 1251insNN, 1252insNN, 1253insNN,
1254insNN, 1255insNN, 1256insNN, 1257insNN, 1258insNN, 1259insNN, 1260insNN,
1261insNN, 1262insNN, 1263insNN, 1264insNN, 1265insNN, 1266insNN, 1267insNN,
1268insNN, 1269insNN, 1270insNN, 1271insNN, 1272insNN, 1273insNN, 1274insNN,
1275insNN, 1276insNN, 1277insNN, 1278insNN, 1279insNN, 1280insNN, 1281insNN,
1282insNN, 1283insNN, 1284insNN, 1285insNN, 1286insNN, 1287insNN, 1288insNN,
1289insNN, 1290insNN, 1291insNN, 1292insNN, 1293insNN, 1294insNN, 1295insNN,
1296insNN, 1297insNN, 1298insNN, 1299insNN, 1300insNN, 1301insNN, 1302insNN,
1303insNN, 1304insNN, 1305insNN, 1306insNN, 1307insNN, 1308insNN, 1309insNN,
1310insNN, 1311insNN, 1312insNN, 1313insNN, 1314insNN, 1315insNN, 1316insNN,
1317insNN, 1318insNN, 1319insNN, 1320insNN, 1321insNN, 1322insNN, 1323insNN,
1324insNN, 1325insNN, 1326insNN, 1327insNN, 1328insNN, 1329insNN, 1330insNN,
1331insNN, 1332insNN, 1333insNN, 1334insNN, 1335insNN, 1336insNN, 1337insNN,
1338insNN, 1339insNN, 1340insNN, 1341insNN, 1342insNN, 1343insNN, 1344insNN,
1345insNN, 1346insNN, 1347insNN, 1348insNN, 1349insNN, 1350insNN, 1351insNN,
1352insNN, 1353insNN, 1354insNN, 1355insNN, 1356insNN, 1357insNN, 1358insNN,
1359insNN, 1360insNN, 1361insNN, 1362insNN, 1363insNN, 1364insNN, 1365insNN,
1366insNN, 1367insNN, 1368insNN, 1369insNN, 1370insNN, 1371insNN, 1372insNN,
1373insNN, 1374insNN, 1375insNN, 1376insNN, 1377insNN, 1378insNN, 1379insNN,
1380insNN, 1381insNN, 1382insNN, 1383insNN, 1384insNN, 1385insNN, 1386insNN,
1387insNN, 1388insNN, 1389insNN, 1390insNN, 1391insNN, 1392insNN, 1393insNN,
1394insNN, 1395insNN, 1396insNN, 1397insNN, 1398insNN, 1399insNN, 1400insNN,
1401insNN, 1402insNN, 1403insNN, 1404insNN, 1405insNN, 1406insNN, 1407insNN,
1408insNN, 1409insNN, 1410insNN, 1411insNN, 1412insNN, 1413insNN, 1414insNN,
1415insNN, 1416insNN, 1417insNN, 1418insNN, 1419insNN, 1420insNN, 1421insNN,
1422insNN, 1423insNN, 1424insNN, 1425insNN, 1426insNN, 1427insNN, 1428insNN,
1429insNN, 1430insNN, 1431insNN, 1432insNN, 1433insNN, 1434insNN, 1435insNN,
1436insNN, 1437insNN, 1438insNN, 1439insNN, 1440insNN, 1441insNN, 1442insNN,
1443insNN, 1444insNN, 1445insNN, 1446insNN, 1447insNN, 1448insNN, 1449insNN,
1450insNN, 1451insNN, 1452insNN, 1453insNN, 1454insNN, 1455insNN, 1456insNN,
1457insNN, 1458insNN, 1459insNN, 1460insNN, 1461insNN, 1462insNN, 1463insNN,
1464insNN, 1465insNN, 1466insNN, 1467insNN, 1468insNN, 1469insNN, 1470insNN,
1471insNN, 1472insNN, 1473insNN, 1474insNN, 1475insNN, 1476insNN, 1477insNN,
1478insNN, 1479insNN, 1480insNN, 1481insNN, 1482insNN, 1483insNN, 1484insNN,
1485insNN, 1486insNN, 1487insNN, 1488insNN, 1489insNN, 1490insNN, 1491insNN,
1492insNN, 1493insNN, 1494insNN, 1495insNN, 1496insNN, 1497insNN, 1498insNN,
1499insNN, 1500insNN, 1501insNN, 1502insNN, 1503insNN, 1504insNN, 1505insNN,
1506insNN, 1507insNN, 1508insNN, 1509insNN, 1510insNN, 1511insNN, 1512insNN,
1513insNN, 1514insNN, 1515insNN, 1516insNN, 1517insNN, 1518insNN, 1519insNN,
1520insNN, 1521insNN, 1522insNN, 1523insNN, 1524insNN, 1525insNN, 1526insNN,
1527insNN, 1528insNN, 1529insNN, 1530insNN, 1531insNN, 1532insNN, 1533insNN,
1534insNN, 1535insNN, 1536insNN, 1537insNN, 1538insNN, 1539insNN, 1540insNN,
1541insNN, 1542insNN, 1543insNN, 1544insNN, 1545insNN, 1546insNN, 1547insNN,
1548insNN, 1549insNN, 1550insNN, 1551insNN, 1552insNN, 1553insNN, 1554insNN,
1555insNN, 1556insNN, 1557insNN, 1558insNN, 1559insNN, 1560insNN, 1561insNN,
1562insNN, 1563insNN, 1564insNN, 1565insNN, 1566insNN, 1567insNN, 1568insNN,
1569insNN, 1570insNN, 1571insNN, 1572insNN, 1573insNN, 1574insNN, 1575insNN,
1576insNN, 1577insNN, 1578insNN, 1579insNN, 1580insNN, 1581insNN, 1582insNN,
1583insNN, 1584insNN, 1585insNN, 1586insNN, 1587insNN, 1588insNN, 1589insNN,
1590insNN, 1591insNN, 1592insNN, 1593insNN, 1594insNN, 1595insNN, 1596insNN,
1597insNN, 1598insNN, 1599insNN, 1600insNN, 1601insNN, 1602insNN, 1603insNN,
1604insNN, 1605insNN, 1606insNN, 1607insNN, 1608insNN, 1609insNN, 1610insNN,
1611insNN, 1612insNN, 1613insNN, 1614insNN, 1615insNN, 1616insNN, 1617insNN,
1618insNN, 1619insNN, 1620insNN, 1621insNN, 1622insNN, 1623insNN, 1624insNN,
1625insNN, 1626insNN, 1627insNN, 1628insNN, 1629insNN, 1630insNN, 1631insNN,
1632insNN, 1633insNN, 1634insNN, 1635insNN, 1636insNN, 1637insNN, 1638insNN,
1639insNN, 1640insNN, 1641insNN, 1642insNN, 1643insNN, 1644insNN, 1645insNN,
1646insNN, 1647insNN, 1648insNN, 1649insNN, 1650insNN, 1651insNN, 1652insNN,
1653insNN, 1654insNN, 1655insNN, 1656insNN, 1657insNN, 1658insNN, 1659insNN,
1660insNN, 1661insNN, 1662insNN, 1663insNN, 1664insNN, 1665insNN, 1666insNN,
1667insNN, 1668insNN, 1669insNN, 1670insNN, 1671insNN, 1672insNN, 1673insNN,
1674insNN, 1675insNN, 1676insNN, 1677insNN, 1678insNN, 1679insNN, 1680insNN,
1681insNN, 1682insNN, 1683insNN, 1684insNN, 1685insNN, 1686insNN, 1687insNN,
1688insNN, 1689insNN, 1690insNN, 1691insNN, 1692insNN, 1693insNN, 1694insNN,
1695insNN, 1696insNN, 1697insNN, 1698insNN, 1699insNN, 1700insNN, 1701insNN,
1702insNN, 1703insNN, 1704insNN, 1705insNN, 1706insNN, 1707insNN, 1708insNN,
1709insNN, 1710insNN, 1711insNN, 1712insNN, 1713insNN, 1714insNN, 1715insNN,
1716insNN, 1717insNN, 1718insNN, 1719insNN, 1720insNN, 1721insNN, 1722insNN,
1723insNN, 1724insNN, 1725insNN, 1726insNN, 1727insNN, 1728insNN, 1729insNN,
1730insNN, 1731insNN, 1732insNN, 1733insNN, 1734insNN, 1735insNN, 1736insNN,
1737insNN, 1738insNN, 1739insNN, 1740insNN, 1741insNN, 1742insNN, 1743insNN,
1744insNN, 1745insNN, 1746insNN, 1747insNN, 1748insNN, 1749insNN, 1750insNN,
1751insNN, 1752insNN, 1753insNN, 1754insNN, 1755insNN, 1756insNN, 1757insNN,
1758insNN, 1759insNN, 1760insNN, 1761insNN, 1762insNN, 1763insNN, 1764insNN,
1765insNN, 1766insNN, 1767insNN, 1768insNN, 1769insNN, 1770insNN, 1771insNN,
1772insNN, 1773insNN, 1774insNN, 1775insNN, 1776insNN, 1777insNN, 1778insNN,
1779insNN, 1780insNN, 1781insNN, 1782insNN, 1783insNN, 1784insNN, 1785insNN,
1786insNN, 1787insNN, 1788insNN, 1789insNN, 1790insNN, 1791insNN, 1792insNN,
1793insNN, 1794insNN, 1795insNN, 1796insNN, 1797insNN, 1798insNN, 1799insNN,
1800insNN, 1801insNN, 1802insNN, 1803insNN, 1804insNN, 1805insNN, 1806insNN,
1807insNN, 1808insNN, 1809insNN, 1810insNN, 1811insNN, 1812insNN, 1813insNN,
1814insNN, 1815insNN, 1816insNN, 1817insNN, 1818insNN, 1819insNN, 1820insNN,
1821insNN, 1822insNN, 1823insNN, 1824insNN, 1825insNN, 1826insNN, 1827insNN,
1828insNN, 1829insNN, 1830insNN, 1831insNN, 1832insNN, 1833insNN, 1834insNN,
1835insNN, 1836insNN, 1837insNN, 1838insNN, 1839insNN, 1840insNN, 1841insNN,
1842insNN, 1843insNN, 1844insNN, 1845insNN, 1846insNN, 1847insNN, 1848insNN,
1849insNN, 1850insNN, 1851insNN, 1852insNN, 1853insNN, 1854insNN, 1855insNN,
1856insNN, 1857insNN, 1858insNN, 1859insNN, 1860insNN, 1861insNN, 1862insNN,
1863insNN, 1864insNN, 1865insNN, 1866insNN, 1867insNN, 1868insNN, 1869insNN,
1870insNN, 1871insNN, 1872insNN, 1873insNN, 1874insNN, 1875insNN, 1876insNN,
1877insNN, 1878insNN, 1879insNN, 1880insNN, 1881insNN, 1882insNN, 1883insNN,
1884insNN, 1885insNN, 1886insNN, 1887insNN, 1888insNN, 1889insNN, 1890insNN,
1891insNN, 1892insNN, 1893insNN, 1894insNN, 1895insNN, 1896insNN, 1897insNN,
1898insNN, 1899insNN, 1900insNN, 1901insNN, 1902insNN, 1903insNN, 1904insNN,
1905insNN, 1906insNN, 1907insNN, 1908insNN, 1909insNN, 1910insNN, 1911insNN,
1912insNN, 1913insNN, 1914insNN, 1915insNN, 1916insNN, 1917insNN, 1918insNN,
1919insNN, 1920insNN, 1921insNN, 1922insNN, 1923insNN, 1924insNN, 1925insNN,
1926insNN, 1927insNN, 1928insNN, 1929insNN, 1930insNN, 1931insNN, 1932insNN,
1933insNN, 1934insNN, 1935insNN, 1936insNN, 1937insNN, 1938insNN, 1939insNN,
1940insNN, 1941insNN, 1942insNN, 1943insNN, 1944insNN, 1945insNN, 1946insNN,
1947insNN, 1948insNN, 1949insNN, 1950insNN, 1951insNN, 1952insNN, 1953insNN,
1954insNN, 1955insNN, 1956insNN, 1957insNN, 1958insNN, 1959insNN, 1960insNN,
1961insNN, 1962insNN, 1963insNN, 1964insNN, 1965insNN, 1966insNN, 1967insNN,
1968insNN, 1969insNN, 1970insNN, 1971insNN, 1972insNN, 1973insNN, 1974insNN,
1975insNN, 1976insNN, 1977insNN, 1978insNN, 1979insNN, 1980insNN, 1981insNN,
1982insNN, 1983insNN, 1984insNN, 1985insNN, 1986insNN, 1987insNN, 1988insNN,
1989insNN, 1990insNN, 1991insNN, 1992insNN, 1993insNN, 1994insNN, 1995insNN,
1996insNN, 1997insNN, 1998insNN, 1999insNN, 2000insNN, 2001insNN, 2002insNN,
2003insNN, 2004insNN, 2005insNN, 2006insNN, 2007insNN, 2008insNN, 2009insNN,
2010insNN, 2011insNN, 2012insNN, 2013insNN, 2014insNN, 2015insNN, 2016insNN,
2017insNN, 2018insNN, 2019insNN, 2020insNN, 2021insNN, 2022insNN, 2023insNN,
2024insNN, 2025insNN, 2026insNN, 2027insNN, 2028insNN, 2029insNN, 2030insNN,
2031insNN, 2032insNN, 2033insNN, 2034insNN, 2035insNN, 2036insNN, 2037insNN,
2038insNN, 2039insNN, 2040insNN, 2041insNN, 2042insNN, 2043insNN, 2044insNN,
2045insNN, 2046insNN, 2047insNN, 2048insNN, 2049insNN, 2050insNN, 2051insNN,
2052insNN, 2053insNN, 2054insNN, 2055insNN, 2056insNN, 2057insNN, 2058insNN,
2059insNN, 2060insNN, 2061insNN, 2062insNN, 2063insNN, 2064insNN, 2065insNN,
2066insNN, 2067insNN, 2068insNN, 2069insNN, 2070insNN, 2071insNN, 2072insNN,
2073insNN, 2074insNN, 2075insNN, 2076insNN, 2077insNN, 2078insNN, 2079insNN,
2080insNN, 2081insNN, 2082insNN, 2083insNN, 2084insNN, 2085insNN, 2086insNN,
2087insNN, 2088insNN, 2089insNN, 2090insNN, 2091insNN, 2092insNN, 2093insNN,
2094insNN, 2095insNN, 2096insNN, 2097insNN, 2098insNN, 2099insNN, 2100insNN,
2101insNN, 2102insNN, 2103insNN, 2104insNN, 2105insNN, 2106insNN, 2107insNN,
2108insNN, 2109insNN, 2110insNN, 2111insNN, 2112insNN, 2113insNN, 2114insNN,
2115insNN, 2116insNN, 2117insNN, 2118insNN, 2119insNN, 2120insNN, 2121insNN,
2122insNN, 2123insNN, 2124insNN, 2125insNN, 2126insNN, 2127insNN, 2128insNN,
2129insNN, 2130insNN, 2131insNN, 2132insNN, 2133insNN, 2134insNN, 2135insNN,
2136insNN, 2137insNN, 2138insNN, 2139insNN, 2140insNN, 2141insNN, 2142insNN,
2143insNN, 2144insNN, 2145insNN, 2146insNN, 2147insNN, 2148insNN, 2149insNN,
2150insNN, 2151insNN, 2152insNN, 2153insNN, 2154insNN, 2155insNN, 2156insNN,
2157insNN, 2158insNN, 2159insNN, 2160insNN, 2161insNN, 2162insNN, 2163insNN,
2164insNN, 2165insNN, 2166insNN, 2167insNN, 2168insNN, 2169insNN, 2170insNN,
2171insNN, 2172insNN, 2173insNN, 2174insNN, 2175insNN, 2176insNN, 2177insNN,
2178insNN, 2179insNN, 2180insNN, 2181insNN, 2182insNN, 2183insNN, 2184insNN,
2185insNN, 2186insNN, 2187insNN, 2188insNN, 2189insNN, 2190insNN, 2191insNN,
2192insNN, 2193insNN, 2194insNN, 2195insNN, 2196insNN, 2197insNN, 2198insNN,
2199insNN, 2200insNN, 2201insNN, 2202insNN, 2203insNN, 2204insNN, 2205insNN,
2206insNN, 2207insNN, 2208insNN, 2209insNN, 2210insNN, 2211insNN, 2212insNN,
2213insNN, 2214insNN, 2215insNN, 2216insNN, 2217insNN, 2218insNN, 2219insNN,
2220insNN, 2221insNN, 2222insNN, 2223insNN, 2224insNN, 2225insNN, 2226insNN,
2227insNN, 2228insNN, 2229insNN, 2230insNN, 2231insNN, 2232insNN, 2233insNN,
2234insNN, 2235insNN, 2236insNN, 2237insNN, 2238insNN, 2239insNN, 2240insNN,
2241insNN, 2242insNN, 2243insNN, 2244insNN, 2245insNN, 2246insNN, 2247insNN,
2248insNN, 2249insNN, 2250insNN, 2251insNN, 2252insNN, 2253insNN, 2254insNN,
2255insNN, 2256insNN, 2257insNN, 2258insNN, 2259insNN, 2260insNN, 2261insNN,
2262insNN, 2263insNN, 2264insNN, 2265insNN, 2266insNN, 2267insNN, 2268insNN,
2269insNN, 2270insNN, 2271insNN, 2272insNN, 2273insNN, 2274insNN, 2275insNN,
2276insNN, 2277insNN, 2278insNN, 2279insNN, 2280insNN, 2281insNN, 2282insNN,
2283insNN, 2284insNN, 2285insNN, 2286insNN, 2287insNN, 2288insNN, 2289insNN,
2290insNN, 2291insNN, 2292insNN, 2293insNN, 2294insNN, 2295insNN, 2296insNN,
2297insNN, 2298insNN, 2299insNN, 2300insNN, 2301insNN, 2302insNN, 2303insNN,
2304insNN, 2305insNN, 2306insNN, 2307insNN, 2308insNN, 2309insNN, 2310insNN,
2311insNN, 2312insNN, 2313insNN, 2314insNN, 2315insNN, 2316insNN, 2317insNN,
2318insNN, 2319insNN, 2320insNN, 2321insNN, 2322insNN, 2323insNN, 2324insNN,
2325insNN, 2326insNN, 2327insNN, 2328insNN, 2329insNN, 2330insNN, 2331insNN,
2332insNN, 2333insNN, 2334insNN, 2335insNN, 2336insNN, 2337insNN, 2338insNN,
2339insNN, 2340insNN, 2341insNN, 2342insNN, 2343insNN, 2344insNN, 2345insNN,
2346insNN, 2347insNN, 2348insNN, 2349insNN, 2350insNN, 2351insNN, 2352insNN,
2353insNN, 2354insNN, 2355insNN, 2356insNN, 2357insNN, 2358insNN, 2359insNN,
2360insNN, 2361insNN, 2362insNN, 2363insNN, 2364insNN, 2365insNN, 2366insNN,
2367insNN, 2368insNN, 2369insNN, 2370insNN, 2371insNN, 2372insNN, 2373insNN,
2374insNN, 2375insNN, 2376insNN, 2377insNN, 2378insNN, 2379insNN, 2380insNN,
2381insNN, 2382insNN, 2383insNN, 2384insNN, 2385insNN, 2386insNN, 2387insNN,
2388insNN, 2389insNN, 2390insNN, 2391insNN, 2392insNN, 2393insNN, 2394insNN,
2395insNN, 2396insNN, 2397insNN, 2398insNN, 2399insNN, 2400insNN, 2401insNN,
2402insNN, 2403insNN, 2404insNN, 2405insNN, 2406insNN, 2407insNN, 2408insNN,
2409insNN, 2410insNN, 2411insNN, 2412insNN, 2413insNN, 2414insNN, 2415insNN,
2416insNN, 2417insNN, 2418insNN, 2419insNN, 2420insNN, 2421insNN, 2422insNN,
2423insNN, 2424insNN, 2425insNN, 2426insNN, 2427insNN, 2428insNN, 2429insNN,
2430insNN, 2431insNN, 2432insNN, 2433insNN, 2434insNN, 2435insNN, 2436insNN,
2437insNN, 2438insNN, 2439insNN, 2440insNN, 2441insNN, 2442insNN, 2443insNN,
2444insNN, 2445insNN, 2446insNN, 2447insNN, 2448insNN, 2449insNN, 2450insNN,
2451insNN, 2452insNN, 2453insNN, 2454insNN, 2455insNN, 2456insNN, 2457insNN,
2458insNN, 2459insNN, 2460insNN, 2461insNN, 2462insNN, 2463insNN, 2464insNN,
2465insNN, 2466insNN, 2467insNN, 2468insNN, 2469insNN, 2470insNN, 2471insNN,
2472insNN, 2473insNN, 2474insNN, 2475insNN, 2476insNN, 2477insNN, 2478insNN,
2479insNN, 2480insNN, 2481insNN, 2482insNN, 2483insNN, 2484insNN, 2485insNN,
2486insNN, 2487insNN, 2488insNN, 2489insNN, 2490insNN, 2491insNN, 2492insNN,
2493insNN, 2494insNN, 2495insNN, 2496insNN, 2497insNN, 2498insNN, 2499insNN,
2500insNN, 2501insNN, 2502insNN, 2503insNN, 2504insNN, 2505insNN, 2506insNN,
2507insNN, 2508insNN, 2509insNN, 2510insNN, 2511insNN, 2512insNN, 2513insNN,
2514insNN, 2515insNN, 2516insNN, 2517insNN, 2518insNN, 2519insNN, 2520insNN,
2521insNN, 2522insNN, 2523insNN, 2524insNN, 2525insNN, 2526insNN, 2527insNN,
2528insNN, 2529insNN, 2530insNN, 2531insNN, 2532insNN, 2533insNN, 2534insNN,
2535insNN, 2536insNN, 2537insNN, 2538insNN, 2539insNN, 2540insNN, 2541insNN,
2542insNN, 2543insNN, 2544insNN, 2545insNN, 2546insNN, 2547insNN, 2548insNN,
2549insNN, 2550insNN, 2551insNN, 2552insNN, 2553insNN, 2554insNN, 2555insNN,
2556insNN, 2557insNN, 2558insNN, 2559insNN, 2560insNN, 2561insNN, 2562insNN,
2563insNN, 2564insNN, 2565insNN, 2566insNN, 2567insNN, 2568insNN, 2569insNN,
2570insNN, 2571insNN, 2572insNN, 2573insNN, 2574insNN, 2575insNN, 2576insNN,
2577insNN, 2578insNN, 2579insNN, 2580insNN, 2581insNN, 2582insNN, 2583insNN,
2584insNN, 2585insNN, 2586insNN, 2587insNN, 2588insNN, 2589insNN, 2590insNN,
2591insNN, 2592insNN, 2593insNN, 2594insNN, 2595insNN, 2596insNN, 2597insNN,
2598insNN, 2599insNN, 2600insNN, 2601insNN, 2602insNN, 2603insNN, 2604insNN,
2605insNN, 2606insNN, 2607insNN, 2608insNN, 2609insNN, 2610insNN, 2611insNN,
2612insNN, 2613insNN, 2614insNN, 2615insNN, 2616insNN, 2617insNN, 2618insNN,
2619insNN, 2620insNN, 2621insNN, 2622insNN, 2623insNN, 2624insNN, 2625insNN,
2626insNN, 2627insNN, 2628insNN, 2629insNN, 2630insNN, 2631insNN, 2632insNN,
2633insNN, 2634insNN, 2635insNN, 2636insNN, 2637insNN, 2638insNN, 2639insNN,
2640insNN, 2641insNN, 2642insNN, 2643insNN, 2644insNN, 2645insNN, 2646insNN,
2647insNN, 2648insNN, 2649insNN, 2650insNN, 2651insNN, 2652insNN, 2653insNN,
2654insNN, 2655insNN, 2656insNN, 2657insNN, 2658insNN, 2659insNN, 2660insNN,
2661insNN, 2662insNN, 2663insNN, 2664insNN, 2665insNN, 2666insNN, 2667insNN,
2668insNN, 2669insNN, 2670insNN, 2671insNN, 2672insNN, 2673insNN, 2674insNN,
2675insNN, 2676insNN, 2677insNN, 2678insNN, 2679insNN, 2680insNN, 2681insNN,
2682insNN, 2683insNN, 2684insNN, 2685insNN, 2686insNN, 2687insNN, 2688insNN,
2689insNN, 2690insNN, 2691insNN, 2692insNN, 2693insNN, 2694insNN, 2695insNN,
2696insNN, 2697insNN, 2698insNN, 2699insNN, 2700insNN, 2701insNN, 2702insNN,
2703insNN, 2704insNN, 2705insNN, 2706insNN, 2707insNN, 2708insNN, 2709insNN,
2710insNN, 2711insNN, 2712insNN, 2713insNN, 2714insNN, 2715insNN, 2716insNN,
2717insNN, 2718insNN, 2719insNN, 2720insNN, 2721insNN, 2722insNN, 2723insNN,
2724insNN, 2725insNN, 2726insNN, 2727insNN, 2728insNN, 2729insNN, 2730insNN,
2731insNN, 2732insNN, 2733insNN, 2734insNN, 2735insNN, 2736insNN, 2737insNN,
2738insNN, 2739insNN, 2740insNN, 2741insNN, 2742insNN, 2743insNN, 2744insNN,
2745insNN, 2746insNN, 2747insNN, 2748insNN, 2749insNN, 2750insNN, 2751insNN,
2752insNN, 2753insNN, 2754insNN, 2755insNN, 2756insNN, 2757insNN, 2758insNN,
2759insNN, 2760insNN, 2761insNN, 2762insNN, 2763insNN, 2764insNN, 2765insNN,
2766insNN, 2767insNN, 2768insNN, 2769insNN, 2770insNN, 2771insNN, 2772insNN,
2773insNN, 2774insNN, 2775insNN, 2776insNN, 2777insNN, 2778insNN, 2779insNN,
2780insNN, 2781insNN, 2782insNN, 2783insNN, 2784insNN, 2785insNN, 2786insNN,
2787insNN, 2788insNN, 2789insNN, 2790insNN, 2791insNN, 2792insNN, 2793insNN,
2794insNN, 2795insNN, 2796insNN, 2797insNN, 2798insNN, 2799insNN, 2800insNN,
2801insNN, 2802insNN, 2803insNN, 2804insNN, 2805insNN, 2806insNN, 2807insNN,
2808insNN, 2809insNN, 2810insNN, 2811insNN, 2812insNN, 2813insNN, 2814insNN,
2815insNN, 2816insNN, 2817insNN, 2818insNN, 2819insNN, 2820insNN, 2821insNN,
2822insNN, 2823insNN, 2824insNN, 2825insNN, 2826insNN, 2827insNN, 2828insNN,
2829insNN, 2830insNN, 2831insNN, 2832insNN, 2833insNN, 2834insNN, 2835insNN,
2836insNN, 2837insNN, 2838insNN, 2839insNN, 2840insNN, 2841insNN, 2842insNN,
2843insNN, 2844insNN, 2845insNN, 2846insNN, 2847insNN, 2848insNN, 2849insNN,
2850insNN, 2851insNN, 2852insNN, 2853insNN, 2854insNN, 2855insNN, 2856insNN,
2857insNN, 2858insNN, 2859insNN, 2860insNN, 2861insNN, 2862insNN, 2863insNN,
2864insNN, 2865insNN, 2866insNN, 2867insNN, 2868insNN, 2869insNN, 2870insNN,
2871insNN, 2872insNN, 2873insNN, 2874insNN, 2875insNN, 2876insNN, 2877insNN,
2878insNN, 2879insNN, 2880insNN, 2881insNN, 2882insNN, 2883insNN, 2884insNN,
2885insNN, 2886insNN, 2887insNN, 2888insNN, 2889insNN, 2890insNN, 2891insNN,
2892insNN, 2893insNN, 2894insNN, 2895insNN, 2896insNN, 2897insNN, 2898insNN,
2899insNN, 2900insNN, 2901insNN, 2902insNN, 2903insNN, 2904insNN, 2905insNN,
2906insNN, 2907insNN, 2908insNN, 2909insNN, 2910insNN, 2911insNN, 2912insNN,
2913insNN, 2914insNN, 2915insNN, 2916insNN, 2917insNN, 2918insNN, 2919insNN,
2920insNN, 2921insNN, 2922insNN, 2923insNN, 2924insNN, 2925insNN, 2926insNN,
2927insNN, 2928insNN, 2929insNN, 2930insNN, 2931insNN, 2932insNN, 2933insNN,
2934insNN, 2935insNN, 2936insNN, 2937insNN, 2938insNN, 2939insNN, 2940insNN,
2941insNN, 2942insNN, 2943insNN, 2944insNN, 2945insNN, 2946insNN, 2947insNN,
2948insNN, 2949insNN, 2950insNN, 2951insNN, 2952insNN, 2953insNN, 2954insNN,
2955insNN, 2956insNN, 2957insNN, 2958insNN, 2959insNN, 2960insNN, 2961insNN,
2962insNN, 2963insNN, 2964insNN, 2965insNN, 2966insNN, 2967insNN, 2968insNN,
2969insNN, 2970insNN, 2971insNN, 2972insNN, 2973insNN, 2974insNN, 2975insNN,
2976insNN, 2977insNN, 2978insNN, 2979insNN, 2980insNN, 2981insNN, 2982insNN,
2983insNN, 2984insNN, 2985insNN, 2986insNN, 2987insNN, 2988insNN, 2989insNN,
2990insNN, 2991insNN, 2992insNN, 2993insNN, 2994insNN, 2995insNN, 2996insNN,
2997insNN, 2998insNN, 2999insNN, 3000insNN, 3001insNN, 3002insNN, 3003insNN,
3004insNN, 3005insNN, 3006insNN, 3007insNN, 3008insNN, 3009insNN, 3010insNN,
3011insNN, 3012insNN, 3013insNN, 3014insNN, 3015insNN, 3016insNN, 3017insNN,
3018insNN, 3019insNN, 3020insNN, 3021insNN, 3022insNN, 3023insNN, 3024insNN,
3025insNN, 3026insNN, 3027insNN, 3028insNN, 3029insNN, 3030insNN, 3031insNN,
3032insNN, 3033insNN, 3034insNN, 3035insNN, 3036insNN, 3037insNN, 3038insNN,
3039insNN, 3040insNN, 3041insNN, 3042insNN, 3043insNN, 3044insNN, 3045insNN,
3046insNN, 3047insNN, 3048insNN, 3049insNN, 3050insNN, 3051insNN, 3052insNN,
3053insNN, 3054insNN, 3055insNN, 3056insNN, 3057insNN, 3058insNN, 3059insNN,
3060insNN, 3061insNN, 3062insNN, 3063insNN, 3064insNN, 3065insNN, 3066insNN,
3067insNN, 3068insNN, 3069insNN, 3070insNN, 3071insNN, 3072insNN, 3073insNN,
3074insNN, 3075insNN, 3076insNN, 3077insNN, 3078insNN, 3079insNN, 3080insNN,
3081insNN, 3082insNN, 3083insNN, 3084insNN, 3085insNN, 3086insNN, 3087insNN,
3088insNN, 3089insNN, 3090insNN, 3091insNN, 3092insNN, 3093insNN, 3094insNN,
3095insNN, 3096insNN, 3097insNN, 3098insNN, 3099insNN, 3100insNN, 3101insNN,
3102insNN, 3103insNN, 3104insNN, 3105insNN, 3106insNN, 3107insNN, 3108insNN,
3109insNN, 3110insNN, 3111insNN, 3112insNN, 3113insNN, 3114insNN, 3115insNN,
3116insNN, 3117insNN, 3118insNN, 3119insNN, 3120insNN, 3121insNN, 3122insNN,
3123insNN, 3124insNN, 3125insNN, 3126insNN, 3127insNN, 3128insNN, 3129insNN,
3130insNN, 3131insNN, 3132insNN, 3133insNN, 3134insNN, 3135insNN, 3136insNN,
3137insNN, 3138insNN, 3139insNN, 3140insNN, 3141insNN, 3142insNN, 3143insNN,
3144insNN, 3145insNN, 3146insNN, 3147insNN, 3148insNN, 3149insNN, 3150insNN,
3151insNN, 3152insNN, 3153insNN, 3154insNN, 3155insNN, 3156insNN, 3157insNN,
3158insNN, 3159insNN, 3160insNN, 3161insNN, 3162insNN, 3163insNN, 3164insNN,
3165insNN, 3166insNN, 3167insNN, 3168insNN, 3169insNN, 3170insNN, 3171insNN,
3172insNN, 3173insNN, 3174insNN, 3175insNN, 3176insNN, 3177insNN, 3178insNN,
3179insNN, 3180insNN, 3181insNN, 3182insNN, 3183insNN, 3184insNN, 3185insNN,
3186insNN, 3187insNN, 3188insNN, 3189insNN, 3190insNN, 3191insNN, 3192insNN,
3193insNN, 3194insNN, 3195insNN, 3196insNN, 3197insNN, 3198insNN, 3199insNN,
3200insNN, 3201insNN, 3202insNN, 3203insNN, 3204insNN, 3205insNN, 3206insNN,
3207insNN, 3208insNN, 3209insNN, 3210insNN, 3211insNN, 3212insNN, 3213insNN,
3214insNN, 3215insNN, 3216insNN, 3217insNN, 3218insNN, 3219insNN, 3220insNN,
3221insNN, 3222insNN, 3223insNN, 3224insNN, 3225insNN, 3226insNN, 3227insNN,
3228insNN, 3229insNN, 3230insNN, 3231insNN, 3232insNN, 3233insNN, 3234insNN,
3235insNN, 3236insNN, 3237insNN, 3238insNN, 3239insNN, 3240insNN, 3241insNN,
3242insNN, 3243insNN, 3244insNN, 3245insNN, 3246insNN, 3247insNN, 3248insNN,
3249insNN, 3250insNN, 3251insNN, 3252insNN, 3253insNN, 3254insNN, 3255insNN,
3256insNN, 3257insNN, 3258insNN, 3259insNN, 3260insNN, 3261insNN, 3262insNN,
3263insNN, 3264insNN, 3265insNN, 3266insNN, 3267insNN, 3268insNN, 3269insNN,
3270insNN, 3271insNN, 3272insNN, 3273insNN, 3274insNN, 3275insNN, 3276insNN,
3277insNN, 3278insNN, 3279insNN, 3280insNN, 3281insNN, 3282insNN, 3283insNN,
3284insNN, 3285insNN, 3286insNN, 3287insNN, 3288insNN, 3289insNN, 3290insNN,
3291insNN, 3292insNN, 3293insNN, 3294insNN, 3295insNN, 3296insNN, 3297insNN,
3298insNN, 3299insNN, 3300insNN, 3301insNN, 3302insNN, 3303insNN, 3304insNN,
3305insNN, 3306insNN, 3307insNN, 3308insNN, 3309insNN, 3310insNN, 3311insNN,
3312insNN, 3313insNN, 3314insNN, 3315insNN, 3316insNN, 3317insNN, 3318insNN,
3319insNN, 3320insNN, 3321insNN, 3322insNN, 3323insNN, 3324insNN, 3325insNN,
3326insNN, 3327insNN, 3328insNN, 3329insNN, 3330insNN, 3331insNN, 3332insNN,
3333insNN, 3334insNN, 3335insNN, 3336insNN, 3337insNN, 3338insNN, 3339insNN,
3340insNN, 3341insNN, 3342insNN, 3343insNN, 3344insNN, 3345insNN, 3346insNN,
3347insNN, 3348insNN, 3349insNN, 3350insNN, 3351insNN, 3352insNN, 3353insNN,
3354insNN, 3355insNN, 3356insNN, 3357insNN, 3358insNN, 3359insNN, 3360insNN,
3361insNN, 3362insNN, 3363insNN, 3364insNN, 3365insNN, 3366insNN, 3367insNN,
3368insNN, 3369insNN, 3370insNN, 3371insNN, 3372insNN, 3373insNN, 3374insNN,
3375insNN, 3376insNN, 3377insNN, 3378insNN, 3379insNN, 3380insNN, 3381insNN,
3382insNN, 3383insNN, 3384insNN, 3385insNN, 3386insNN, 3387insNN, 3388insNN,
3389insNN, 3390insNN, 3391insNN, 3392insNN, 3393insNN, 3394insNN, 3395insNN,
3396insNN, 3397insNN, 3398insNN, 3399insNN, 3400insNN, 3401insNN, 3402insNN,
3403insNN, 3404insNN, 3405insNN, 3406insNN, 3407insNN, 3408insNN, 3409insNN,
3410insNN, 3411insNN, 3412insNN, 3413insNN, 3414insNN, 3415insNN, 3416insNN,
3417insNN, 3418insNN, 3419insNN, 3420insNN, 3421insNN, 3422insNN, 3423insNN,
3424insNN, 3425insNN, 3426insNN, 3427insNN, 3428insNN, 3429insNN, 3430insNN,
3431insNN, 3432insNN, 3433insNN, 3434insNN, 3435insNN, 3436insNN, 3437insNN,
3438insNN, 3439insNN, 3440insNN, 3441insNN, 3442insNN, 3443insNN, 3444insNN,
3445insNN, 3446insNN, 3447insNN, 3448insNN, 3449insNN, 3450insNN, 3451insNN,
3452insNN, 3453insNN, 3454insNN, 3455insNN, 3456insNN, 3457insNN, 3458insNN,
3459insNN, 3460insNN, 3461insNN, 3462insNN, 3463insNN, 3464insNN, 3465insNN,
3466insNN, 3467insNN, 3468insNN, 3469insNN, 3470insNN, 3471insNN, 3472insNN,
3473insNN, 3474insNN, 3475insNN, 3476insNN, 3477insNN, 3478insNN, 3479insNN,
3480insNN, 3481insNN, 3482insNN, 3483insNN, 3484insNN, 3485insNN, 3486insNN,
3487insNN, 3488insNN, 3489insNN, 3490insNN, 3491insNN, 3492insNN, 3493insNN,
3494insNN, 3495insNN, 3496insNN, 3497insNN, 3498insNN, 3499insNN, 3500insNN,
3501insNN, 3502insNN, 3503insNN, 3504insNN, 3505insNN, 3506insNN, 3507insNN,
3508insNN, 3509insNN, 3510insNN, 3511insNN, 3512insNN, 3513insNN, 3514insNN,
3515insNN, 3516insNN, 3517insNN, 3518insNN, 3519insNN, 3520insNN, 3521insNN,
3522insNN, 3523insNN, 3524insNN, 3525insNN, 3526insNN, 3527insNN, 3528insNN,
3529insNN, 3530insNN, 3531insNN, 3532insNN, 3533insNN, 3534insNN, 3535insNN,
3536insNN, 3537insNN, 3538insNN, 3539insNN, 3540insNN, 3541insNN, 3542insNN,
3543insNN, 3544insNN, 3545insNN, 3546insNN, 3547insNN, 3548insNN, 3549insNN,
3550insNN, 3551insNN, 3552insNN, 3553insNN, 3554insNN, 3555insNN, 3556insNN,
3557insNN, 3558insNN, 3559insNN, 3560insNN, 3561insNN, 3562insNN, 3563insNN,
3564insNN, 3565insNN, 3566insNN, 3567insNN, 3568insNN, 3569insNN, 3570insNN,
3571insNN, 3572insNN, 3573insNN, 3574insNN, 3575insNN, 3576insNN, 3577insNN,
3578insNN, 3579insNN, 3580insNN, 3581insNN, 3582insNN, 3583insNN, 3584insNN,
3585insNN, 3586insNN, 3587insNN, 3588insNN, 3589insNN, 3590insNN, 3591insNN,
3592insNN, 3593insNN, 3594insNN, 3595insNN, 3596insNN, 3597insNN, 3598insNN,
3599insNN, 3600insNN, 3601insNN, 3602insNN, 3603insNN, 3604insNN, 3605insNN,
3606insNN, 3607insNN, 3608insNN, 3609insNN, 3610insNN, 3611insNN, 3612insNN,
3613insNN, 3614insNN, 3615insNN, 3616insNN, 3617insNN, 3618insNN, 3619insNN,
3620insNN, 3621insNN, 3622insNN, 3623insNN, 3624insNN, 3625insNN, 3626insNN,
3627insNN, 3628insNN, 3629insNN, 3630insNN, 3631insNN, 3632insNN, 3633insNN,
3634insNN, 3635insNN, 3636insNN, 3637insNN, 3638insNN, 3639insNN, 3640insNN,
3641insNN, 3642insNN, 3643insNN, 3644insNN, 3645insNN, 3646insNN, 3647insNN,
3648insNN, 3649insNN, 3650insNN, 3651insNN, 3652insNN, 3653insNN, 3654insNN,
3655insNN, 3656insNN, 3657insNN, 3658insNN, 3659insNN, 3660insNN, 3661insNN,
3662insNN, 3663insNN, 3664insNN, 3665insNN, 3666insNN, 3667insNN, 3668insNN,
3669insNN, 3670insNN, 3671insNN, 3672insNN, 3673insNN, 3674insNN, 3675insNN,
3676insNN, 3677insNN, 3678insNN, 3679insNN, 3680insNN, 3681insNN, 3682insNN,
3683insNN, 3684insNN, 3685insNN, 3686insNN, 3687insNN, 3688insNN, 3689insNN,
3690insNN, 3691insNN, 3692insNN, 3693insNN, 3694insNN, 3695insNN, 3696insNN,
3697insNN, 3698insNN, 3699insNN, 3700insNN, 3701insNN, 3702insNN, 3703insNN,
3704insNN, 3705insNN, 3706insNN, 3707insNN, 3708insNN, 3709insNN, 3710insNN,
3711insNN, 3712insNN, 3713insNN, 3714insNN, 3715insNN, 3716insNN, 3717insNN,
3718insNN, 3719insNN, 3720insNN, 3721insNN, 3722insNN, 3723insNN, 3724insNN,
3725insNN, 3726insNN, 3727insNN, 3728insNN, 3729insNN, 3730insNN, 3731insNN,
3732insNN, 3733insNN, 3734insNN, 3735insNN, 3736insNN, 3737insNN, 3738insNN,
3739insNN, 3740insNN, 3741insNN, 3742insNN, 3743insNN, 3744insNN, 3745insNN,
3746insNN, 3747insNN, 3748insNN, 3749insNN, 3750insNN, 3751insNN, 3752insNN,
3753insNN, 3754insNN, 3755insNN, 3756insNN, 3757insNN, 3758insNN, 3759insNN,
3760insNN, 3761insNN, 3762insNN, 3763insNN, 3764insNN, 3765insNN, 3766insNN,
3767insNN, 3768insNN, 3769insNN, 3770insNN, 3771insNN, 3772insNN, 3773insNN,
3774insNN, 3775insNN, 3776insNN, 3777insNN, 3778insNN, 3779insNN, 3780insNN,
3781insNN, 3782insNN, 3783insNN, 3784insNN, 3785insNN, 3786insNN, 3787insNN,
3788insNN, 3789insNN, 3790insNN, 3791insNN, 3792insNN, 3793insNN, 3794insNN,
3795insNN, 3796insNN, 3797insNN, 3798insNN, 3799insNN, 3800insNN, 3801insNN,
3802insNN, 3803insNN, 3804insNN, 3805insNN, 3806insNN, 3807insNN, 3808insNN,
3809insNN, 3810insNN, 3811insNN, 3812insNN, 3813insNN, 3814insNN, 3815insNN,
3816insNN, 3817insNN, 3818insNN, 3819insNN, 3820insNN, 3821insNN, 3822insNN,
3823insNN, 3824insNN, 3825insNN, 3826insNN, 3827insNN, 3828insNN, 3829insNN,
3830insNN, 3831insNN, 3832insNN, 3833insNN, 3834insNN, 3835insNN, 3836insNN,
3837insNN, 3838insNN, 3839insNN, 3840insNN, 3841insNN, 3842insNN, 3843insNN,
3844insNN, 3845insNN, 3846insNN, 3847insNN, 3848insNN, 3849insNN, 3850insNN,
3851insNN, 3852insNN, 3853insNN, 3854insNN, 3855insNN, 3856insNN, 3857insNN,
3858insNN, 3859insNN, 3860insNN, 3861insNN, 3862insNN, 3863insNN, 3864insNN,
3865insNN, 3866insNN, 3867insNN, 3868insNN, 3869insNN, 3870insNN, 3871insNN,
3872insNN, 3873insNN, 3874insNN, 3875insNN, 3876insNN, 3877insNN, 3878insNN,
3879insNN, 3880insNN, 3881insNN, 3882insNN, 3883insNN, 3884insNN, 3885insNN,
3886insNN, 3887insNN, 3888insNN, 3889insNN, 3890insNN, 3891insNN, 3892insNN,
3893insNN, 3894insNN, 3895insNN, 3896insNN, 3897insNN, 3898insNN, 3899insNN,
3900insNN, 3901insNN, 3902insNN, 3903insNN, 3904insNN, 3905insNN, 3906insNN,
3907insNN, 3908insNN, 3909insNN, 3910insNN, 3911insNN, 3912insNN, 3913insNN,
3914insNN, 3915insNN, 3916insNN, 3917insNN, 3918insNN, 3919insNN, 3920insNN,
3921insNN, 3922insNN, 3923insNN, 3924insNN, 3925insNN, 3926insNN, 3927insNN,
3928insNN, 3929insNN, 3930insNN, 3931insNN, 3932insNN, 3933insNN, 3934insNN,
3935insNN, 3936insNN, 3937insNN, 3938insNN, 3939insNN, 3940insNN, 3941insNN,
3942insNN, 3943insNN, 3944insNN, 3945insNN, 3946insNN, 3947insNN, 3948insNN,
3949insNN, 3950insNN, 3951insNN, 3952insNN, 3953insNN, 3954insNN, 3955insNN,
3956insNN, 3957insNN, 3958insNN, 3959insNN, 3960insNN, 3961insNN, 3962insNN,
3963insNN, 3964insNN, 3965insNN, 3966insNN, 3967insNN, 3968insNN, 3969insNN,
3970insNN, 3971insNN, 3972insNN, 3973insNN, 3974insNN, 3975insNN, 3976insNN,
3977insNN, 3978insNN, 3979insNN, 3980insNN, 3981insNN, 3982insNN, 3983insNN,
3984insNN, 3985insNN, 3986insNN, 3987insNN, 3988insNN, 3989insNN, 3990insNN,
3991insNN, 3992insNN, 3993insNN, 3994insNN, 3995insNN, 3996insNN, 3997insNN,
3998insNN, 3999insNN, 4000insNN, 4001insNN, 4002insNN, 4003insNN, 4004insNN,
4005insNN, 4006insNN, 4007insNN, 4008insNN, 4009insNN, 4010insNN, 4011insNN,
4012insNN, 4013insNN, 4014insNN, 4015insNN, 4016insNN, 4017insNN, 4018insNN,
4019insNN, 4020insNN, 4021insNN, 4022insNN, 4023insNN, 4024insNN, 4025insNN,
4026insNN, 4027insNN, 4028insNN, 4029insNN, 4030insNN, 4031insNN, 4032insNN,
4033insNN, 4034insNN, 4035insNN, 4036insNN, 4037insNN, 4038insNN, 4039insNN,
4040insNN, 4041insNN, 4042insNN, 4043insNN, 4044insNN, 4045insNN, 4046insNN,
4047insNN, 4048insNN, 4049insNN, 4050insNN, 4051insNN, 4052insNN, 4053insNN,
4054insNN, 4055insNN, 4056insNN, 4057insNN, 4058insNN, 4059insNN, 4060insNN,
4061insNN, 4062insNN, 4063insNN, 4064insNN, 4065insNN, 4066insNN, 4067insNN,
4068insNN, 4069insNN, 4070insNN, 4071insNN, 4072insNN, 4073insNN, 4074insNN,
4075insNN, 4076insNN, 4077insNN, 4078insNN, 4079insNN, 4080insNN, 4081insNN,
4082insNN, 4083insNN, 4084insNN, 4085insNN, 4086insNN, 4087insNN, 4088insNN,
4089insNN, 4090insNN, 4091insNN, 4092insNN, 4093insNN, 4094insNN, 4095insNN,
4096insNN, 4097insNN, 4098insNN, 4099insNN, 4100insNN, 4101insNN, 4102insNN,
4103insNN, 4104insNN, 4105insNN, 4106insNN, 4107insNN, 4108insNN, 4109insNN,
4110insNN, 4111insNN, 4112insNN, 4113insNN, 4114insNN, 4115insNN, 4116insNN,
4117insNN, 4118insNN, 4119insNN, 4120insNN, 4121insNN, 4122insNN, 4123insNN,
4124insNN, 4125insNN, 4126insNN, 4127insNN, 4128insNN, 4129insNN, 4130insNN,
4131insNN, 4132insNN, 4133insNN, 4134insNN, 4135insNN, 4136insNN, 4137insNN,
4138insNN, 4139insNN, 4140insNN, 4141insNN, 4142insNN, 4143insNN, 4144insNN,
4145insNN, 4146insNN, 4147insNN, 4148insNN, 4149insNN, 4150insNN, 4151insNN,
4152insNN, 4153insNN, 4154insNN, 4155insNN, 4156insNN, 4157insNN, 4158insNN,
4159insNN, 4160insNN, 4161insNN, 4162insNN, 4163insNN, 4164insNN, 4165insNN,
4166insNN, 4167insNN, 4168insNN, 4169insNN, 4170insNN, 4171insNN, 4172insNN,
4173insNN, 4174insNN, 4175insNN, 4176insNN, 4177insNN, 4178insNN, 4179insNN,
4180insNN, 4181insNN, 4182insNN, 4183insNN, 4184insNN, 4185insNN, 4186insNN,
4187insNN, 4188insNN, 4189insNN, 4190insNN, 4191insNN, 4192insNN, 4193insNN,
4194insNN, 4195insNN, 4196insNN, 4197insNN, 4198insNN, 4199insNN, 4200insNN,
4201insNN, 4202insNN, 4203insNN, 4204insNN, 4205insNN, 4206insNN, 4207insNN,
4208insNN, 4209insNN, 4210insNN, 4211insNN, 4212insNN, 4213insNN, 4214insNN,
4215insNN, 4216insNN, 4217insNN, 4218insNN, 4219insNN, 4220insNN, 4221insNN,
4222insNN, 4223insNN, 4224insNN, 4225insNN, 4226insNN, 4227insNN, 4228insNN,
4229insNN, 4230insNN, 4231insNN, 4232insNN, 4233insNN, 4234insNN, 4235insNN,
4236insNN, 4237insNN, 4238insNN, 4239insNN, 4240insNN, 4241insNN, 4242insNN,
4243insNN, 4244insNN, 4245insNN, 4246insNN, 4247insNN, 4248insNN, 4249insNN,
4250insNN, 4251insNN, 4252insNN, 4253insNN, 4254insNN, 4255insNN, 4256insNN,
4257insNN, 4258insNN, 4259insNN, 4260insNN, 4261insNN, 4262insNN, 4263insNN,
4264insNN, 4265insNN, 4266insNN, 4267insNN, 4268insNN, 4269insNN, 4270insNN,
4271insNN, 4272insNN, 4273insNN, 4274insNN, 4275insNN, 4276insNN, 4277insNN,
4278insNN, 4279insNN, 4280insNN, 4281insNN, 4282insNN, 4283insNN, 4284insNN,
4285insNN, 4286insNN, 4287insNN, 4288insNN, 4289insNN, 4290insNN, 4291insNN,
4292insNN, 4293insNN, 4294insNN, 4295insNN, 4296insNN, 4297insNN, 4298insNN,
4299insNN, 4300insNN, 4301insNN, 4302insNN, 4303insNN, 4304insNN, 4305insNN,
4306insNN, 4307insNN, 4308insNN, 4309insNN, 4310insNN, 4311insNN, 4312insNN,
4313insNN, 4314insNN, 4315insNN, 4316insNN, 4317insNN, 4318insNN, 4319insNN,
4320insNN, 4321insNN, 4322insNN, 4323insNN, 4324insNN, 4325insNN, 4326insNN,
4327insNN, 4328insNN, 4329insNN, 4330insNN, 4331insNN, 4332insNN, 4333insNN,
4334insNN, 4335insNN, 4336insNN, 4337insNN, 4338insNN, 4339insNN, 4340insNN,
4341insNN, 4342insNN, 4343insNN, 4344insNN, 4345insNN, 4346insNN, 4347insNN,
4348insNN, 4349insNN, 4350insNN, 4351insNN, 4352insNN, 4353insNN, 4354insNN,
4355insNN, 4356insNN, 4357insNN, 4358insNN, 4359insNN, 4360insNN, 4361insNN,
4362insNN, 4363insNN, 4364insNN, 4365insNN, 4366insNN, 4367insNN, 4368insNN,
4369insNN, 4370insNN, 4371insNN, 4372insNN, 4373insNN, 4374insNN, 4375insNN,
4376insNN, 4377insNN, 4378insNN, 4379insNN, 4380insNN, 4381insNN, 4382insNN,
4383insNN, 4384insNN, 4385insNN, 4386insNN, 4387insNN, 4388insNN, 4389insNN,
4390insNN, 4391insNN, 4392insNN, 4393insNN, 4394insNN, 4395insNN, 4396insNN,
4397insNN, 4398insNN, 4399insNN, 4400insNN, 4401insNN, 4402insNN, 4403insNN,
4404insNN, 4405insNN, 4406insNN, 4407insNN, 4408insNN, 4409insNN, 4410insNN,
4411insNN, 4412insNN, 4413insNN, 4414insNN, 4415insNN, 4416insNN, 4417insNN,
4418insNN, 4419insNN, 4420insNN, 4421insNN, 4422insNN, 4423insNN, 4424insNN,
4425insNN, 4426insNN, 4427insNN, 4428insNN, 4429insNN, 4430insNN, 4431insNN,
4432insNN, 4433insNN, 4434insNN, 4435insNN, 4436insNN, 4437insNN, 4438insNN,
4439insNN, 4440insNN, 4441insNN, 4442insNN, 4443insNN, 4444insNN, 4445insNN,
4446insNN, 4447insNN, 4448insNN, 4449insNN, 4450insNN, 4451insNN, 4452insNN,
4453insNN, 4454insNN, 4455insNN, 4456insNN, 4457insNN, 4458insNN, 4459insNN,
4460insNN, 4461insNN, 4462insNN, 4463insNN, 4464insNN, 4465insNN, 4466insNN,
4467insNN, 4468insNN, 4469insNN, 4470insNN, 4471insNN, 4472insNN, 4473insNN,
4474insNN, 4475insNN, 4476insNN, 4477insNN, 4478insNN, 4479insNN, 4480insNN,
4481insNN, 4482insNN, 4483insNN, 4484insNN, 4485insNN, 4486insNN, 4487insNN,
4488insNN, 4489insNN, 4490insNN, 4491insNN, 4492insNN, 4493insNN, 4494insNN,
4495insNN, 4496insNN, 4497insNN, 4498insNN, 4499insNN, 4500insNN, 4501insNN,
4502insNN, 4503insNN, 4504insNN, 4505insNN, 4506insNN, 4507insNN, 4508insNN,
4509insNN, 4510insNN, 4511insNN, 4512insNN, 4513insNN, 4514insNN, 4515insNN,
4516insNN, 4517insNN, 4518insNN, 4519insNN, 4520insNN, 4521insNN, 4522insNN,
4523insNN, 4524insNN, 4525insNN, 4526insNN, 4527insNN, 4528insNN, 4529insNN,
4530insNN, 4531insNN, 4532insNN, 4533insNN, 4534insNN, 4535insNN, 4536insNN,
4537insNN, 4538insNN, 4539insNN, 4540insNN, 4541insNN, 4542insNN, 4543insNN,
4544insNN, 4545insNN, 4546insNN, 4547insNN, 4548insNN, 4549insNN, 4550insNN,
4551insNN, 4552insNN, 4553insNN, 4554insNN, 4555insNN, 4556insNN, 4557insNN,
4558insNN, 4559insNN, 4560insNN, 4561insNN, 4562insNN, 4563insNN, 4564insNN,
4565insNN, 4566insNN, 4567insNN, 4568insNN, 4569insNN, 4570insNN, 4571insNN,
4572insNN, 4573insNN, 4574insNN, 4575insNN, 4576insNN, 4577insNN, 4578insNN,
4579insNN, 4580insNN, 4581insNN, 4582insNN, 4583insNN, 4584insNN, 4585insNN,
4586insNN, 4587insNN, 4588insNN, 4589insNN, 4590insNN, 4591insNN, 4592insNN,
4593insNN, 4594insNN, 4595insNN, 4596insNN, 4597insNN, 4598insNN, 4599insNN,
4600insNN, 4601insNN, 4602insNN, 4603insNN, 4604insNN, 4605insNN, 4606insNN,
4607insNN, 4608insNN, 4609insNN, 4610insNN, 4611insNN, 4612insNN, 4613insNN,
4614insNN, 4615insNN, 4616insNN, 4617insNN, 4618insNN, 4619insNN, 4620insNN,
4621insNN, 4622insNN, 4623insNN, 4624insNN, 4625insNN, 4626insNN, 4627insNN,
4628insNN, 4629insNN, 4630insNN, 4631insNN, 4632insNN, 4633insNN, 4634insNN,
4635insNN, 4636insNN, 4637insNN, 4638insNN, 4639insNN, 4640insNN, 4641insNN,
4642insNN, 4643insNN, 4644insNN, 4645insNN, 4646insNN, 4647insNN, 4648insNN,
4649insNN, 4650insNN, 4651insNN, 4652insNN, 4653insNN, 4654insNN, 4655insNN,
4656insNN, 4657insNN, 4658insNN, 4659insNN, 4660insNN, 4661insNN, 4662insNN,
4663insNN, 4664insNN, 4665insNN, 4666insNN, 4667insNN, 4668insNN, 4669insNN,
4670insNN, 4671insNN, 4672insNN, 4673insNN, 4674insNN, 4675insNN, 4676insNN,
4677insNN, 4678insNN, 4679insNN, 4680insNN, 4681insNN, 4682insNN, 4683insNN,
4684insNN, 4685insNN, 4686insNN, 4687insNN, 4688insNN, 4689insNN, 4690insNN,
4691insNN, 4692insNN, 4693insNN, 4694insNN, 4695insNN, 4696insNN, 4697insNN,
4698insNN, 4699insNN, 4700insNN, 4701insNN, 4702insNN, 4703insNN, 4704insNN,
4705insNN, 4706insNN, 4707insNN, 4708insNN, 4709insNN, 4710insNN, 4711insNN,
4712insNN, 4713insNN, 4714insNN, 4715insNN, 4716insNN, 4717insNN, 4718insNN,
4719insNN, 4720insNN, 4721insNN, 4722insNN, 4723insNN, 4724insNN, 4725insNN,
4726insNN, 4727insNN, 4728insNN, 4729insNN, 4730insNN, 4731insNN, 4732insNN,
4733insNN, 4734insNN, 4735insNN, 4736insNN, 4737insNN, 4738insNN, 4739insNN,
4740insNN, 4741insNN, 4742insNN, 4743insNN, 4744insNN, 4745insNN, 4746insNN,
4747insNN, 4748insNN, 4749insNN, 4750insNN, 4751insNN, 4752insNN, 4753insNN,
4754insNN, 4755insNN, 4756insNN, 4757insNN, 4758insNN, 4759insNN, 4760insNN,
4761insNN, 4762insNN, 4763insNN, 4764insNN, 4765insNN, 4766insNN, 4767insNN,
4768insNN, 4769insNN, 4770insNN, 4771insNN, 4772insNN, 4773insNN, 4774insNN,
4775insNN, 4776insNN, 4777insNN, 4778insNN, 4779insNN, 4780insNN, 4781insNN,
4782insNN, 4783insNN, 4784insNN, 4785insNN, 4786insNN, 4787insNN, 4788insNN,
4789insNN, 4790insNN, 4791insNN, 4792insNN, 4793insNN, 4794insNN, 4795insNN,
4796insNN, 4797insNN, 4798insNN, 4799insNN, 4800insNN, 4801insNN, 4802insNN,
4803insNN, 4804insNN, 4805insNN, 4806insNN, 4807insNN, 4808insNN, 4809insNN,
4810insNN, 4811insNN, 4812insNN, 4813insNN, 4814insNN, 4815insNN, 4816insNN,
4817insNN, 4818insNN, 4819insNN, 4820insNN, 4821insNN, 4822insNN, 4823insNN,
4824insNN, 4825insNN, 4826insNN, 4827insNN, 4828insNN, 4829insNN, 4830insNN,
4831insNN, 4832insNN, 4833insNN, 4834insNN, 4835insNN, 4836insNN, 4837insNN,
4838insNN, 4839insNN, 4840insNN, 4841insNN, 4842insNN, 4843insNN, 4844insNN,
4845insNN, 4846insNN, 4847insNN, 4848insNN, 4849insNN, 4850insNN, 4851insNN,
4852insNN, 4853insNN, 4854insNN, 4855insNN, 4856insNN, 4857insNN, 4858insNN,
4859insNN, 4860insNN, 4861insNN, 4862insNN, 4863insNN, 4864insNN, 4865insNN,
4866insNN, 4867insNN, 4868insNN, 4869insNN, 4870insNN, 4871insNN, 4872insNN,
4873insNN, 4874insNN, 4875insNN, 4876insNN, 4877insNN, 4878insNN, 4879insNN,
4880insNN, 4881insNN, 4882insNN, 4883insNN, 4884insNN, 4885insNN, 4886insNN,
4887insNN, 4888insNN, 4889insNN, 4890insNN, 4891insNN, 4892insNN, 4893insNN,
4894insNN, 4895insNN, 4896insNN, 4897insNN, 4898insNN, 4899insNN, 4900insNN,
4901insNN, 4902insNN, 4903insNN, 4904insNN, 4905insNN, 4906insNN, 4907insNN,
4908insNN, 4909insNN, 4910insNN, 4911insNN, 4912insNN, 4913insNN, 4914insNN,
4915insNN, 4916insNN, 4917insNN, 4918insNN, 4919insNN, 4920insNN, 4921insNN,
4922insNN, 4923insNN, 4924insNN, 4925insNN, 4926insNN, 4927insNN, 4928insNN,
4929insNN, 4930insNN, 4931insNN, 4932insNN, 4933insNN, 4934insNN, 4935insNN,
4936insNN, 4937insNN, 4938insNN, 4939insNN, 4940insNN, 4941insNN, 4942insNN,
4943insNN, 4944insNN, 4945insNN, 4946insNN, 4947insNN, 4948insNN, 4949insNN,
4950insNN, 4951insNN, 4952insNN, 4953insNN, 4954insNN, 4955insNN, 4956insNN,
4957insNN, 4958insNN, 4959insNN, 4960insNN, 4961insNN, 4962insNN, 4963insNN,
4964insNN, 4965insNN, 4966insNN, 4967insNN, 4968insNN, 4969insNN, 4970insNN,
4971insNN, 4972insNN, 4973insNN, 4974insNN, 4975insNN, 4976insNN, 4977insNN,
4978insNN, 4979insNN, 4980insNN, 4981insNN, 4982insNN, 4983insNN, 4984insNN,
4985insNN, 4986insNN, 4987insNN, 4988insNN, 4989insNN, 4990insNN, 4991insNN,
4992insNN, 4993insNN, 4994insNN, 4995insNN, 4996insNN, 4997insNN, 4998insNN,
4999insNN, 5000insNN, 5001insNN, 5002insNN, 5003insNN, 5004insNN, 5005insNN,
5006insNN, 5007insNN, 5008insNN, 5009insNN, 5010insNN, 5011insNN, 5012insNN,
5013insNN, 5014insNN, 5015insNN, 5016insNN, 5017insNN, 5018insNN, 5019insNN,
5020insNN, 5021insNN, 5022insNN, 5023insNN, 5024insNN, 5025insNN, 5026insNN,
5027insNN, 5028insNN, 5029insNN, 5030insNN, 5031insNN, 5032insNN, 5033insNN,
5034insNN, 5035insNN, 5036insNN, 5037insNN, 5038insNN, 5039insNN, 5040insNN,
5041insNN, 5042insNN, 5043insNN, 5044insNN, 5045insNN, 5046insNN, 5047insNN,
5048insNN, 5049insNN, 5050insNN, 5051insNN, 5052insNN, 5053insNN, 5054insNN,
5055insNN, 5056insNN, 5057insNN, 5058insNN, 5059insNN, 5060insNN, 5061insNN,
5062insNN, 5063insNN, 5064insNN, 5065insNN, 5066insNN, 5067insNN, 5068insNN,
5069insNN, 5070insNN, 5071insNN, 5072insNN, 5073insNN, 5074insNN, 5075insNN,
5076insNN, 5077insNN.

[0231] The present invention is not to be limited in scope by the specific embodiments described herein, which are intended as single illustrations of individual aspects of the invention, and functionally equivalent methods and components are within the scope of the invention. Indeed, various modifications of the invention, in addition to those shown and described herein will become apparent to those skilled in the art from the foregoing description and accompanying drawings. Such modifications are intended to fall within the scope of the appended claims.

[0232] All references mentioned herein are incorporated by reference.

Claims

1. An isolated mutant BRCA1 gene or polynucleotide fragment thereof containing a mutation site, or a polynucleotide complementary to said gene or said fragment, having an in-frame stop codon before codon 1863, with the proviso that the mutation site not be one defined by TABLE 1.

2. An isolated mutant BRCA1 gene, a polynucleotide fragment of said mutant BRCA1 gene, or a complementary polynucleotide to said mutant BRCA1 gene or said fragment, according to claim 1, containing a truncating mutation and forming a stop codon as defined in TABLES 3-7, wherein the mutant BRCA1 gene codes for a truncated BRCA1 protein which lacks full biological activity of wild-type BRCA1 protein.

3. An isolated mutant BRCA1 gene, a polynucleotide fragment of said mutant BRCA1 gene or a complementary polynucleotide to said mutant BRCA1 gene or said fragment according to claim 1, containing a truncating mutation and having the sequence 5′ R1-R2-R3 3′; where R1 is a wild type BRCA1 DNA sequence from nucleotide number 120 to X, R2 is TAA, TAG or TGA, and R3 is a wild type BRCA1 DNA sequence from nucleotide number X+4 to 5711 and where X=123 to 5710; or R1 is a wild type BRCA1 DNA sequence from nucleotide number 120 to X, R2 is zero nucleotides, and R3 is the wild type BRCA1 DNA sequence from nucleotides X+Y+1 to 5711, where Y is an integer of 3n+1 or 3n+2 where n=0 to 1861 and where X=123 to 5707; or R1 is a wild type BRCA1 DNA sequence from nucleotide number 120 to X, R2 is Y nucleotides of any sequence, and R3 contains the wild type BRCA1 DNA sequence of nucleotide number X+1 to 5711, where Y is 3n+1 or 3n+2 where n is an integer of zero or greater, and where X=123 to 5707; wherein the BRCA1 gene codes for a truncated BRCA1 protein which lacks full biological activity of wild-type BRCA1 protein, with the proviso that the mutation not be one defined by TABLE 1.

4. An isolated mutant BRCA1 gene or a polynucleotide fragment thereof, or a polynucleotide complementary to said mutant BRCA1 gene or said fragment according to claim 1, containing a truncating mutation site and capable of specifically hybridizing to an oligonucleotide probe being at least 12 nucleotides in length and having the sequence 5′ R1-R2-R3 3′; where either R1 contains at its 3′ end three nucleotides complementary to codon X-1 of the wild-type BRCA1 gene; R2 is complementary to TAG, TAA or TGA, and R3 contains at its 5′ end three nucleotides complementary to codon X+1 of the wild type BRCA1 gene, where X=2 to 1862; or R1 contains at its 3′ end three nucleotides complementary to nucleotide numbers X-2 to X of the wild-type BRCA1 gene, R2 is an oligonucleotide having Y nucleotides of any sequence, R3 contains at its 5′ end three nucleotides complementary to nucleotide numbers X+1 to X+3 of the wild type BRCA1 gene, where Y is an integer greater than zero and is not 3 or a multiple of 3, where X=122 to 5707; or R1 contains at its 3′ end three nucleotides complementary to nucleotide numbers X-2 to X of the wild-type BRCA1 gene, R2=zero, R3 contains at its 5′ end three nucleotides complementary to nucleotide numbers X+1+Y of the wild type BRCA1 DNA sequence, where Y=1 to 5582 but is not 3 or a multiple of 3 and where X=122 to 5706; wherein the oligonucleotide probe is unable to specifically hybridize to the wild-type BRCA1 gene; and with the proviso that the mutation not be one defined by TABLE 1.

5. A mutant BRCA1 gene or a polynucleotide fragment thereof, or a polynucleotide complementary to said mutant BRCA1 gene or said fragment according to claim 1, containing a premature stop codon and incapable of expressing a complete BRCA1 protein; wherein said mutant BRCA1 gene contains a mutation resulting from a substituted nucleotide in the naturally occurring (wild-type) sequence so that an in-frame stop codon is formed at any of codon numbers 2-1863; or inserted or deleted 3n+1 or 3n+2 nucleotides, where n is an integer of 0 or greater, causing a frame shift mutation in the naturally occurring (wild-type) sequence so that an in-frame stop codon is formed at any of codon numbers 2-1863; wherein a mutant BRCA1 protein expressed from said mutant BRCA1 gene lacks full biological activity of naturally occurring (wild type) BRCA1 protein; and with the proviso that the mutation is not one of the mutations listed in TABLE 1.

6. A method for detecting a mutation in a sample containing a mutant BRCA1 gene according to claim 1, comprising: a) amplifying at least a fragment of sample BRCA1 gene; b) dermining the sequence of said at least a fragment of the sample BRCA1 gene; and c) comparing the sequence obtained with a wild-type BRCA1 sequence; wherein the presence of the sequence of said mutation in said sample BRCA1 gene indicates the presence of a mutation in the sample.

7. A method for detecting a mutation in a sample containing a BRCA1 gene comprising: a) amplifying at least a fragment of sample BRCA1 gene; b) determining the sequence of said at least a fragment of sample BRCA1 gene; and c) comparing the sequence obtained with one or more sequences of mutant BRCA1 genes according to claim 1;wherein the presence of a sequence of said mutant BRCA1 gene indicates the presence of a mutation in the sample.

8. A method for detecting a mutation in a BRCA1 gene comprising: a) obtaining a BRCA1 protein expressed by a BRCA1 gene according to claim 1; and b) determining the relative molecular weight of said BRCA1 protein compared to wild type BRCA1 protein; c) wherein the presence of said BRCA1 protein having a molecular weight less than that of wild-type BRCA1 protein indicates the presence of a mutation in the BRCA1 gene.

9. An oligonucleotide capable of specifically hybridizing to either: a) a DNA containing a mutant BRCA1 as defined in claim 1, or a DNA having a sequence complementary thereto; or b) a DNA containing a wild-type BRCA1 sequence at a mutation site other than defined by TABLE 1, or a DNA having a sequence complementary thereto; but not both a) and b).

10. A plurality of oligonucleotides comprising at least one oligonucleotide according to claim 9 and at least one additional oligonucleotide capable of specifically hybridizing to the wild-type BRCA1 gene or its complement.

11. A chip array having “n” elements for performing allele specific sequence-based techniques using the oligonucleotide probes comprising, a solid phase chip and a plurality of oligonucleotides of claim 10 having “n” different nucleotide sequences, wherein “n” is an integer greater than one; wherein said oligonucleotides are bound to said solid phase chip in a manner which permits said oligonucleotides to effectively hybridize to complementary oligonucleotides or polynucleotides; wherein oligonucleotides having different nucleotide sequences are bound to said solid phase chip at different locations so that a particular location on said solid phase chip exclusively binds oligonucleotides having a specific nucleotide sequence; and wherein at least one oligonucleotide is capable of specifically hybridizing to a mutant BRCA1 gene having a truncating mutation as defined by TABLES 3-7 or a DNA complementary thereto, with the proviso that the mutation not be one defined by TABLE 1, wherein the BRCA1 gene codes for a truncated BRCA1 protein which lacks full biological activity of wild-type BRCA1 protein.

12. A method for detecting the presence or absence of a BRCA1 gene mutation in a sample DNA comprising, specifically hybridizing sample DNA with an oligonucleotide according to claim 9 under stringent conditions and determining whether said oligonucleotide specifically hybridizes to said sample DNA.

13. A method for determining therapy for an individual having a tumor comprising: a) obtaining a DNA containing biological sample from the individual having a tumor; b) determining whether the DNA contains a mutant BRCA1 gene according to claim 1, or a DNA complementary thereto; and c) determining appropriate therapy based on the presence or absence of said mutant BRCA1 gene; wherein the BRCA1 gene codes for a truncated BRCA1 protein which lacks full biological activity of wild-type BRCA1 protein.

14. A method for determining whether to begin diagnostic or prophylactic treatment for an individual comprising: a) obtaining a DNA containing biological sample from the individual; b) determining whether the DNA contains a mutant BRCA1 gene according to claim 1; and c) determining appropriate diagnostic or prophylactic treatment based on the presence or absence of said mutant BRCA1 gene; wherein the mutant BRCA1 gene codes for a truncated BRCA1 protein which lacks full biological activity of wild-type BRCA1 protein.

15. A method for treating a condition associated with a mutant BRCA1 gene comprising administering biologically active BRCA1 protein to a patient with a condition associated with a mutant BRCA1 gene wherein said patient contains cells having a mutant BRCA1 gene according to claim 1, wherein the BRCA1 gene codes for a truncated BRCA1 protein which lacks full biological activity of wild-type BRCA1 protein.

16. A method for preventing a condition associated with a mutant BRCA1 gene comprising administering biologically active BRCA1 protein to a patient with a cancer wherein said patient contains cells having a truncating BRCA1 gene mutation according to claim 1, wherein the BRCA1 gene codes for a truncated BRCA1 protein which lacks full biological activity of wild-type BRCA1 protein.

17. A method for determining appropriate gene therapy for an individual comprising detecting the presence of a mutant BRCA1 gene according to claim 1, in cells from the individual and administering a DNA containing biologically active BRCA1 gene to the individual, wherein the mutant BRCA1 gene codes for a truncated BRCA1 protein which lacks full biological activity of wild-type BRCA1 protein.

18. A method for detecting a mutation in a sample containing a mutant BRCA1 gene according to claim 2, comprising: a) amplifying at least a fragment of sample BRCA1 gene; b) determining the sequence of said at least a fragment of sample BRCA1 gene; and c) comparing the sequence obtained with a wild-type BRCA1 sequence; wherein the presence of the sequence of said mutant BRCA1 gene indicates the presence of a mutation in the sample.

19. A method for detecting a mutation in a sample containing a BRCA1 gene comprising: a) amplifying at least a fragment of sample BRCA1 gene; b) determining the sequence of said at least a fragment of sample BRCA1 gene; and c) comparing the sequence obtained with one or more of mutant BRCA1 genes according to claim 2;wherein the presence of a sequence of said mutant BRCA1 gene indicates the presence of a mutation in the sample.

20. A method for detecting a mutation in a BRCA1 gene comprising: a) obtaining a BRCA1 protein expressed by a BRCA1 gene according to claim 2; and b) determining the relative molecular weight of said BRCA1 protein compared to wild type BRCA1 protein; wherein the presence of said BRCA1 protein having a molecular weight less than that of wild-type BRCA1 protein indicates the presence of a mutation in the BRCA1 gene.

21. An oligonucleotide capable of specifically hybridizing to either: a) a DNA containing a mutant BRCA1 as defined in claim 2, or a DNA having a sequence complementary thereto; or b) a DNA containing a wild-type BRCA1 sequence at a mutation site other than defined by TABLE 1, or a DNA having a sequence complementary thereto; but not both a) and b).

22. A plurality of oligonucleotides comprising at least one oligonucleotide according to claim 21 and at least one additional oligonucleotide capable of specifically hybridizing to the wild-type BRCA1 gene or its complement.

23. A chip array having “n” elements for performing allele specific sequence-based techniques using the oligonucleotide probes comprising a solid phase chip and a plurality of oligonucleotides of claim 22 having “n” different nucleotide sequences, wherein “n” is an integer greater than two; wherein said oligonucleotides are bound to said solid phase chip in a manner which permits said oligonucleotides to effectively hybridize to complementary oligonucleotides or polynucleotides; wherein oligonucleotides having different nucleotide sequences are bound to said solid phase chip at different locations so that a particular location on said solid phase chip exclusively binds oligonucleotides having a specific nucleotide sequence; and wherein at least one oligonucleotide is capable of specifically hybridizing to a mutant BRCA1 gene having a truncating mutation as defined by TABLES 3-7 or a DNA complementary thereto, with the proviso that the mutation not be one defined by TABLE 1, wherein the BRCA1 gene codes for a truncated BRCA1 protein which lacks full biological activity of wild-type BRCA1 protein.

24. A method for detecting the presence or absence of a BRCA1 gene mutation in a sample DNA comprising specifically hybridizing sample DNA with an oligonucleotide according to claim 21 under stringent conditions and determining whether said oligonucleotide specifically hybridizes to said sample DNA.

25. A method for determining therapy for an individual having a tumor comprising obtaining a DNA containing biological sample from the individual having a tumor, determining whether the DNA contains a mutant BRCA1 gene according to claim 2, or a DNA complementary thereto, and determining appropriate therapy based on the presence or absence of said mutant BRCA1 gene, wherein the BRCA1 gene codes for a truncated BRCA1 protein which lacks full biological activity of wild-type BRCA1 protein.

26. A method for determining whether to begin diagnostic or prophylactic treatment for an individual comprising: a) taking a DNA containing biological sample from the individual; b) determining whether the DNA contains a mutant BRCA1 gene according to claim 2; and c) determining appropriate diagnostic or prophylactic treatment based on the presence or absence of said mutant BRCA1 gene; wherein the mutant BRCA1 gene codes for a truncated BRCA1 protein which lacks full biological activity of wild-type BRCA1 protein.

27. A method for treating a condition associated with a mutant BRCA1 gene comprising, administering biologically active BRCA1 protein to a patient with a condition associated with a mutant BRCA1 gene wherein said patient contains cells having a mutant BRCA1 gene according to claim 2, wherein the BRCA1 gene codes for a truncated BRCA1 protein which lacks full biological activity of wild-type BRCA1 protein.

28. A method for preventing a condition associated with a mutant BRCA1 gene comprising, administering biologically active BRCA1 protein to a patient with a cancer wherein said patient contains cells having a truncating BRCA1 gene mutation according to claim 2, wherein the BRCA1 gene codes for a truncated BRCA1 protein which lacks full biological activity of wild-type BRCA1 protein.

29. A method for determining appropriate gene therapy for an individual comprising, detecting the presence of a mutant BRCA1 gene according to claim 2, in cells from the individual and administering a DNA containing biologically active BRCA1 gene to the individual, wherein the mutant BRCA1 gene codes for a truncated BRCA1 protein which lacks full biological activity of wild-type BRCA1 protein.

30. A method for detecting a mutation in a sample containing a mutant BRCA1 gene according to claim 3, comprising: a) amplifying at least a fragment of sample BRCA1 gene; b) determining the sequence of said at least a fragment of sample BRCA1 gene; and c) comparing the sequence obtained with a wild-type BRCA1 sequence;  wherein the presence of the sequence of said mutant BRCA1 gene indicates the presence of a mutation in the sample.

31. A method for detecting a mutation in a sample containing a BRCA1 gene comprising: a) amplifying at least a fragment of sample BRCA1 gene; b) determining the sequence of said at least a fragment of sample BRCA1 gene; and c) comparing the sequence obtained with one or more of mutant BRCA1 genes according to claim 3;wherein the presence of a sequence of said mutant BRCA1 gene indicates the presence of a mutation in the sample.

32. A method for detecting a mutation in a BRCA1 gene comprising, obtaining a BRCA1 protein expressed by a BRCA1 gene according to claim 3, and determining the relative molecular weight of said BRCA1 protein compared to wild type BRCA1 protein, wherein the presence of said BRCA1 protein having a molecular weight less than that of wild-type BRCA1 protein indicates the presence of a mutation in the BRCA1 gene.

33. An oligonucleotide capable of specifically hybridizing to either: a) a DNA containing a mutant BRCA1 as defined in claim 3, or a DNA having a sequence complementary thereto; or b) a DNA containing a wild-type BRCA1 sequence at a mutation site other than defined by TABLE 1, or a DNA having a sequence complementary thereto; but not both a) and b).

34. A plurality of oligonucleotides comprising at least one oligonucleotide according to claim 33 and at least one additional oligonucleotide capable of specifically hybridizing to the wild-type BRCA1 gene or its complement.

35. A chip array having “n” elements for performing allele specific sequence-based techniques using oligonucleotide probes comprising, a solid phase chip and a plurality of oligonucleotides of claim 34 having “n” different nucleotide sequences, wherein “n” is an integer greater than two; wherein said oligonucleotides are bound to said solid phase chip in a manner which permits said oligonucleotides to effectively hybridize to complementary oligonucleotides or polynucleotides; wherein oligonucleotides having different nucleotide sequences are bound to said solid phase chip at different locations so that a particular location on said solid phase chip exclusively binds oligonucleotides having a specific nucleotide sequence; and wherein at least one oligonucleotide is capable of specifically hybridizing to a mutant BRCA1 gene having a truncating mutation as defined by TABLES 3-7 or a DNA complementary thereto, with the proviso that the mutation not be one defined by TABLE 1, wherein the BRCA1 gene codes for a truncated BRCA1 protein which lacks full biological activity of wild-type BRCA1 protein.

36. A method for detecting the presence or absence of a BRCA1 gene mutation in a sample DNA comprising, specifically hybridizing sample DNA with an oligonucleotide according to claim 33 under stringent conditions, and determining whether said oligonucleotide specifically hybridizes to said sample DNA.

37. A method for determining therapy for an individual having a tumor comprising; a) obtaining a DNA containing biological sample from the individual having a tumor; b) determining whether the DNA contains a mutant BRCA1 gene according to claim 3, or a DNA complementary thereto; and c) determining appropriate therapy based on the presence or absence of said mutant BRCA1 gene; wherein the BRCA1 gene codes for a truncated BRCA1 protein which lacks fill biological activity of wild-type BRCA1 protein.

38. A method for determining whether to begin diagnostic or prophylactic treatment for an individual comprising: a) obtaining a DNA containing biological sample from the individual; b) determining whether the DNA contains a mutant BRCA1 gene according to claim 3; and c) determining appropriate diagnostic or prophylactic treatment based on the presence or absence of said mutant BRCA1 gene; wherein the mutant BRCA1 gene codes for a truncated BRCA1 protein which lacks fill biological activity of wild-type BRCA1 protein.

39. A method for treating a condition associated with a mutant BRCA1 gene comprising,, administering biologically active BRCA1 protein to a patient with a condition associated with a mutant BRCA1 gene wherein said patient contains cells having a mutant BRCA1 gene according to claim 3, wherein the BRCA1 gene codes for a truncated BRCA1 protein which lacks full biological activity of wild-type BRCA1 protein.

40. A method for preventing a condition associated with a mutant BRCA1 gene comprising, administering biologically active BRCA1 protein to a patient with a cancer wherein said patient contains cells having a truncating BRCA1 gene mutation according to claim 3, wherein the BRCA1 gene codes for a truncated BRCA1 protein which lacks full biological activity of wild-type BRCA1 protein.

41. A method for determining appropriate gene therapy for an individual comprising, detecting the presence of a mutant BRCA1 gene according to claim 3 in cells from the individual and administering a DNA containing biologically active BRCA1 gene to the individual, wherein the mutant BRCA1 gene codes for a truncated BRCA1 protein which lacks full biological activity of wild-type BRCA1 protein.

42. A method for detecting a mutation in a sample containing a mutant BRCA1 gene according to claim 4, comprising: a) amplifying at least a fragment of sample BRCA1 gene; b) determining the sequence of said at least a fragment of sample BRCA1 gene; and c) comparing the sequence obtained with a wild-type BRCA1 sequence; wherein the presence of the sequence of said mutant BRCA1 gene indicates the presence of a mutation in the sample.

43. A method for detecting a mutation in a sample containing a BRCA1 gene comprising: a) amplifying at least a fragment of sample BRCA1 gene; b) determining the sequence of said at least a fragment of sample BRCA1 gene; and c) comparing the sequence obtained with one or more of mutant BRCA1 genes according to claim 4;wherein the presence of a sequence of said mutant BRCA1 gene indicates the presence of a mutation in the sample.

44. A method for detecting a mutation in a BRCA1 gene comprising, obtaining a BRCA1 protein expressed by a BRCA1 gene according to claim 4, and determining the relative molecular weight of said BRCA1 protein compared to wild type BRCA1 protein, wherein the presence of said BRCA1 protein having a molecular weight less than that of wild-type BRCA1 protein indicates the presence of a mutation in the BRCA1 gene.

45. An oligonucleotide capable of specifically hybridizing to either: a) a DNA containing a mutant BRCA1 as defined in claim 4, or a DNA having a sequence complementary thereto; or b) a DNA containing a wild-type BRCA1 sequence at a mutation site other than defined by TABLE 1, or a DNA having a sequence complementary thereto; but not both a) and b).

46. A plurality of oligonucleotides comprising at least one oligonucleotide according to claim 45 and at least one additional oligonucleotide capable of specifically hybridizing to the wild-type BRCA1 gene or its complement.

47. A chip array having “n” elements for performing allele specific sequence-based techniques using the oligonucleotide probes comprising, a solid phase chip and a plurality of oligonucleotides of claim 46 having “n” different nucleotide sequences, wherein “n” is an integer greater than two; wherein said oligonucleotides are bound to said solid phase chip in a manner which permits said oligonucleotides to effectively hybridize to complementary oligonucleotides or polynucleotides; wherein oligonucleotides having different nucleotide sequences are bound to said solid phase chip at different locations so that a particular location on said solid phase chip exclusively binds oligonucleotides having a specific nucleotide sequence; and wherein at least one oligonucleotide is capable of specifically hybridizing to a mutant BRCA1 gene having a truncating mutation as defined by TABLES 3-7 or a DNA complementary thereto, with the proviso that the mutation not be one defined by TABLE 1, wherein the BRCA1 gene codes for a truncated BRCA1 protein which lacks full biological activity of wild-type BRCA1 protein.

48. A method for detecting the presence or absence of a BRCA1 gene mutation in a sample DNA comprising, specifically hybridizing sample DNA with an oligonucleotide according to claim 45 under stringent conditions, determining whether said oligonucleotide specifically hybridizes to said sample DNA.

49. A method for determining therapy for an individual having a tumor comprising; a) obtaining a DNA containing biological sample from the individual having a tumor; b) determining whether the DNA contains a mutant BRCA1 gene according to claim 4, or a DNA complementary thereto; and c) determining appropriate therapy based on the presence or absence of said mutant BRCA1 gene; wherein the BRCA1 gene codes for a truncated BRCA1 protein which lacks full biological activity of wild-type BRCA1 protein.

50. A method for determining whether to begin diagnostic or prophylactic treatment for an individual comprising: a) taking a DNA containing biological sample from the individual; b) determining whether the DNA contains a mutant BRCA1 gene according to claim 4; and c) determining appropriate diagnostic or prophylactic treatment based on the presence or absence of said mutant BRCA1 gene; wherein the mutant BRCA1 gene codes for a truncated BRCA1 protein which lacks full biological activity of wild-type BRCA1 protein.

51. A method for treating a condition associated with a mutant BRCA1 gene comprising, administering biologically active BRCA1 protein to a patient with a condition associated with a mutant BRCA1 gene wherein said patient contains cells having a mutant BRCA1 gene according to claim 4, wherein the BRCA1 gene codes for a truncated BRCA1 protein which lacks full biological activity of wild-type BRCA1 protein.

52. A method for preventing a condition associated with a mutant BRCA1 gene comprising, administering biologically active BRCA1 protein to a patient with a cancer, wherein said patient contains cells having a truncating BRCA1 gene mutation according to claim 4, wherein the BRCA1 gene codes for a truncated BRCA1 protein which lacks full biological activity of wild-type BRCA1 protein.

53. A method for determining appropriate gene therapy for an individual comprising, detecting the presence of a mutant BRCA1 gene according to claim 4 in cells from the individual, and administering a DNA containing biologically active BRCA1 gene to the individual, wherein the mutant BRCA1 gene codes for a truncated BRCA1 protein which lacks full biological activity of wild-type BRCA1 protein.

54. A method for detecting a mutation in a sample containing a mutant BRCA1 gene according to claim 5, comprising, amplifying at least a fragment of said BRCA1 gene, determining the sequence of said at least a fragment of said BRCA1 gene, and comparing the sequence obtained with a wild-type BRCA1 sequence, wherein the presence of the sequence of said mutant BRCA1 gene indicates the presence of a mutation in the sample.

55. A method for detecting a mutation in a sample containing a BRCA1 gene comprising, amplifying at least a fragment of said BRCA1 gene, determining the sequence of said at least a fragment of said BRCA1 gene, and comparing the sequence obtained with one or more of mutant BRCA1 genes according to claim 5, wherein the presence of a sequence of said mutant BRCA1 gene indicates the presence of a mutation in the sample.

56. A method for detecting a mutation in a BRCA1 gene comprising, obtaining a BRCA1 protein expressed by a BRCA1 gene according to claim 5, and determining the relative molecular weight of said BRCA1 protein compared to wild type BRCA1 protein, wherein the presence of said BRCA1 protein having a molecular weight less than that of wild-type BRCA1 protein indicates the presence of a mutation in the BRCA1 gene.

57. An oligonucleotide capable of specifically hybridizing to either: a) a DNA containing a mutant BRCA1 as defined in claim 5, or a DNA having a sequence complementary thereto; or b) a DNA containing a wild-type BRCA1 sequence at a mutation site other than defined by TABLE 1, or a DNA having a sequence complementary thereto; but not both a) and b).

58. A plurality of oligonucleotides comprising at least one oligonucleotide according to claim 57 and at least one additional oligonucleotide capable of specifically hybridizing to the wild-type BRCA1 gene or its complement.

59. A chip array having “n” elements for performing allele specific sequence-based techniques using the oligonucleotide probes comprising, a solid phase chip and a plurality of oligonucleotides of claim 58 having “n” different nucleotide sequences, wherein “n” is an integer greater than two; wherein said oligonucleotides are bound to said solid phase chip in a manner which permits said oligonucleotides to effectively hybridize to complementary oligonucleotides or polynucleotides; wherein oligonucleotides having different nucleotide sequences are bound to said solid phase chip at different locations so that a particular location on said solid phase chip exclusively binds oligonucleotides having a specific nucleotide sequence; and wherein at least one oligonucleotide is capable of specifically hybridizing to a mutant BRCA1 gene having a truncating mutation as defined by TABLES 3-7 or a DNA complementary thereto, with the proviso that the mutation not be one defined by TABLE 1, wherein the BRCA1 gene codes for a truncated BRCA1 protein which lacks full biological activity of wild-type BRCA1 protein.

60. A method for detecting the presence or absence of a BRCA1 gene mutation in a sample DNA comprising, specifically hybridizing sample DNA with an oligonucleotide according to claim 57 under stringent conditions, and determining whether said oligonucleotide specifically hybridizes to said sample DNA.

61. A method for determining therapy for an individual having a tumor comprising; a) obtianing a DNA containing biological sample from the individual having a tumor; b) determining whether the DNA contains a mutant BRCA1 gene according to claim 5, or a DNA complementary thereto; and c) determining appropriate therapy based on the presence or absence of said mutant BRCA1 gene; wherein the BRCA1 gene codes for a truncated BRCA1 protein which lacks full biological activity of wild-type BRCA1 protein.

62. A method for determining whether to begin diagnostic or prophylactic treatment for an individual comprising, taking a DNA containing biological sample from the individual, determining whether the DNA contains a mutant BRCA1 gene according to claim 5, and determining appropriate diagnostic or prophylactic treatment based on the presence or absence of said mutant BRCA1 gene, wherein the mutant BRCA1 gene codes for a truncated BRCA1 protein which lacks full biological activity of wild-type BRCA1 protein.

63. A method for treating a condition associated with a mutant BRCA1 gene comprising, administering biologically active BRCA1 protein to a patient with a condition associated with a mutant BRCA1 gene, wherein said patient contains cells having a mutant BRCA1 gene according to claim 5, wherein the BRCA1 gene codes for a truncated BRCA1 protein which lacks full biological activity of wild-type BRCA1 protein.

64. A method for preventing a condition associated with a mutant BRCA1 gene comprising, administering biologically active BRCA1 protein to a patient with a cancer, wherein said patient contains cells having a truncating BRCA1 gene mutation according to claim 5, wherein the BRCA1 gene codes for a truncated BRCA1 protein which lacks full biological activity of wild-type BRCA1 protein.

65. A method for determining appropriate gene therapy for an individual comprising, detecting the presence of a mutant BRCA1 gene according to claim 5 in cells from the individual, and administering a DNA containing biologically active BRCA1 gene to the individual, wherein the mutant BRCA1 gene codes for a truncated BRCA1 protein which lacks full biological activity of wild-type BRCA1 protein.

66. An isolated mutant BRCA1 gene according to claim 1, capable of expressing a truncated BRCA1 protein of less than 1854 amino acids.