Claims
- 1. A method for detecting a mutation which causes or is associated with long QT syndrome in an individual suspected of having long QT syndrome, comprising amplifying the coding sequence of KCNE1 with a first and second primer, wherein the first primer hybridizes to a portion of SEQ ID NO:3 bounded by nucleotides 1 and 174, or its complement, and wherein the second primer hybridizes to a portion of SEQ ID NO:3 bounded by nucleotides 623-1703, or its complement, and analyzing the amplicon for a mutation in the coding sequence of KCNE1 that causes or is associated with long QT syndrome.
- 2. The method of claim 1, wherein the analyzing step further comprises performing the single-stranded conformation polymorphism technique on the amplified sequence.
- 3. The method of claim 1, wherein the analyzing step further comprises sequencing the amplified sequence.
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] The present application is a continuation of application Ser. No. 10/138,316 filed 6 May 2002 which is a division of application Ser. No. 09/444,295 filed 22 Nov. 1999, now U.S. Pat. No. 6,432,644, which is a division of application Ser. No. 09/135,020 filed 17 Aug. 1998, now U.S. Pat. No. 6,274,332, which is continuation-in-part of application Ser. No. 08/921,068 filed 29 Aug. 1997, now abandoned, which is a continuation-in-part of application Ser. No.08/739,383 filed 29 Oct. 1996, now abandoned. Ser. No. 09/135,020 claims priority under 35 USC §119(e) to provisional patent application Serial No. 60/094,477 filed 29 Jul. 1998. Ser. No. 08/739,383 claims priority under 35 USC §119(e) to provisional patent application Ser. No. 60/019,014 filed 22 Dec. 1995. All applications are incorporated herein by reference.
Government Interests
[0002] This application was made with Government support under Grant Nos. RO1 HL48074, and P50-HL52338-02 (SCOR), funded by the National Institutes of Health, Bethesda, Md. The federal government may have certain rights in this invention.
Provisional Applications (2)
|
Number |
Date |
Country |
|
60094477 |
Jul 1998 |
US |
|
60019014 |
Dec 1995 |
US |
Divisions (2)
|
Number |
Date |
Country |
Parent |
09444295 |
Nov 1999 |
US |
Child |
10138316 |
May 2002 |
US |
Parent |
09135020 |
Aug 1998 |
US |
Child |
09444295 |
Nov 1999 |
US |
Continuations (1)
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Number |
Date |
Country |
Parent |
10138316 |
May 2002 |
US |
Child |
10861520 |
Jun 2004 |
US |
Continuation in Parts (2)
|
Number |
Date |
Country |
Parent |
08921068 |
Aug 1997 |
US |
Child |
09135020 |
Aug 1998 |
US |
Parent |
08739383 |
Oct 1996 |
US |
Child |
08921068 |
Aug 1997 |
US |